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Reach your Highest Cloning Efficiency
GenScript's GenBuilder™ DNA Assembly technology takes recombination-based cloning to the next level. Pushing the bounds of innovation, the GenBuilder™ technology allows for seamless fusion of one to twelve gene fragments with the highest efficiency. Whether using fragments longer than 5 kb, unpurified PCR products, single stranded DNA inserts, or have only fifteen minutes to complete the cloning reaction- the GenBuilder™ DNA Assembly technology helps you build your constructs quickly and accurately.
GenBuilder™ perfectly pairs with GenScript's GenPart™ DNA Fragments service. This service enables synthesis of your gene fragments (100-2,000 bp) with delivery in as few as 5 days. The DNA fragments thus generated can be cloned directly into any vector of your choice using the GenBuilder™ DNA Assembly.
DNA Assembly works with unpurified PCR fragments
Incubation in the enzyme mix for only 15 minutes
Rigorously tested among 8 DNA assembly kits
Quality control by assembling 12 fragments with >90% positive rate
Performance verified by >1,000 reactions per day in production line
1. Prepare the DNA fragments with overlaps and the linearized vector for assembly.
2. Add DNA fragments and linearized vectors in the GenBuilder™ master mix. Mix well and incubate at 50° C for 15 minutes.
3. Transform assembly product into competent cells and plate on selective medium. Obtain colonies containing the assembled DNA.
See competitive performance of GenBuilder™ and GenBuilder™ Plus Cloning kits
GenBuilder™ Cloning kit makes synthetic biology easy
Please contact us if you need immediate assistance.
Please contact us (phone: 1-877-436-7274 or email: firstname.lastname@example.org),if you need immediate assistance.