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Reach your Highest Cloning Efficiency
GenScript's GenBuilder™ DNA Assembly technology takes recombination-based cloning to the next level. Pushing the bounds of innovation, the GenBuilder™ technology allows for seamless fusion of one to twelve gene fragments with the highest efficiency. Whether using fragments longer than 5 kb, unpurified PCR products, single stranded DNA inserts, or have only fifteen minutes to complete the cloning reaction- the GenBuilder™ DNA Assembly technology helps you build your constructs quickly and accurately.
GenBuilder™ perfectly pairs with GenScript's GenPart™ DNA Fragments service. This service enables synthesis of your gene fragments (100-2,000 bp) with delivery in as few as 5 days. The DNA fragments thus generated can be cloned directly into any vector of your choice using the GenBuilder™ DNA Assembly.
DNA Assembly works with unpurified PCR fragments
Incubation in the enzyme mix for only 15 minutes
Rigorously tested among 8 DNA assembly kits
Quality control by assembling 12 fragments with >90% positive rate
Performance verified by >1,000 reactions per day in production line
See competitive performance of GenBuilder™ and GenBuilder™ Plus Cloning kits
GenBuilder™ Cloning kit makes synthetic biology easy
For a 1 kb fragment, 400 ng GenPart fragment is generally sufficient for about six GenBuilder reactions.
All GenPart fragments are gel-purified before shipping, so no additional purification is required before using them with the GenBuilder cloning kti.
A 40 bp overlap is optimum for obtaining the maximum cloning efficiency. For cloning one or two fragments into a construct, a 15 bp overlap may be sufficient. For larger number of fragments, we recommend using a 40 bp overlap to increase cloning efficiency. Please see experiment 4.1 (pages 5-6) to see data for cloning together 2 fragments with different over>lap lengths.
We have tested 20 bp ssDNA inserts without issues. For longer ssDNA fragments, the acceptable insert length may have to be determined experimentally.
1. Prepare the DNA fragments with overlaps and the linearized vector for assembly.
2. Add DNA fragments and linearized vectors in the GenBuilder™ master mix. Mix well and incubate at 50° C for 15 minutes.
3. Transform assembly product into competent cells and plate on selective medium. Obtain colonies containing the assembled DNA.
Please contact us if you need immediate assistance.
Please contact us (phone: 1-877-436-7274 or email: firstname.lastname@example.org),if you need immediate assistance.