Overview

GenCircle dsDNA is a novel small circular double stranded DNA vector with a 429bp vector backbone for use as an efficient knock-in template, viral packaging plasmid, or non-viral vector. Both the antibiotic resistance gene and bacterial origin sequence have been removed, and the plasmid will not replicate outside of the engineered production host strain.

Standard plasmid vectors contain >1.5 kb backbones and antibiotic resistance genes that risk conferring drug resistance when used for in vivo applications. Smaller vector backbones generate lower cytotoxicity and immunogenicity, as well as offer better stability and higher transfection efficiency. See how GenCircle dsDNA containing your target gene can improve the safety and efficiency of your gene & cell therapy study.

Applications Field

Cell Therapy

Transposons vector
CRISPR KI template

Gene Therapy

AAV/LV packaging plasmid
non-viral GOI vector
mRNA preparation template

DNA Vaccine

Antigen expression vector

Disease Model
Gene Function Study

Transposons vector
CRISPR KI template

Advantages

Safer! No Antibiotic Resistance Gene

Safer! No Antibiotic Resistance Gene


  • No antibiotic resistance gene & antibiotic residual
  • Prevent plasmid replication outside of the engineered production host strain
Simpler! Lower Cytotoxicity & Immunogenicity

Simpler! Lower Cytotoxicity & Immunogenicity


  • Eliminate CpG islands and other bacterial origin sequences
  • Increase stability for longer persistence
Smaller! Proprietary high fidelity 400bp+ backbone  

Smaller! High fidelity 429bp backbone  


  • Higher gene expression from equivalent plasmid quantity
  • Achieve higher transfection efficiency
More Efficient! Increase KI/viral packaging/GOI expression efficiency

More Efficient! Increase KI/viral packaging/GOI expression efficiency


  • Increase knock-in efficiency by up to 30%
  • Improve viral titer up to 3 folds
  • Increase transcription levels up to 4 folds, and protein expression levels up to 39%

Service Details

Service Grade GOI Length* Quantity* Format TAT Application
GenCircle dsDNA Industrial Grade 1-50 kb 100 μg - g Freeze-dried powder/ Solution liquid (Buffer is optional) From 11 Days
  • Transposons/Transposase vector
  • Viral packaging plasmid
  • GOI transcription & expression
Pre-clinical Grade
  • CRISPR KI template

* For other GOI length & quantity request, please email [email protected] or call 1-877-436-7274.

Service options include:
1. GenScript provides GOI gene synthesis service, and then provides GenCircle dsDNA preparation service.
2. Customer provides GOI in plasmid, then GenScript provides GenCircle dsDNA preparation service.

QC Standards

QC Item QC Standards Industrial Grade Pre-clinical Grade
Sanger Sequencing Correct sequence
Restriction Analysis Conforming to reference pattern
Spectrophotometer Delivery quantity guarantee
Nanodrop UV Value A260/280 = 1.8-2.0
Electrophoresis Non-detectable RNA/genome DNA residual
Homogeneity ≥90% Supercoiled
TAL Endotoxin Quantify <0.01 EU/μg
≤ 0.005 EU/μg
Bioburden Testing No growth on agar plate after 48 hours
pH 8.0±0.5 (in TE buffer)
Residual E. coli DNA Quantitative PCR ≤ 5%
Residual Host Protein HCP ELISA ≤ 1%

Case Studies

Successful Delivered Case

GenScript has successfully delivered multiple orders with a wide range of GOI lengths, including multiple complex or difficult-to-synthesize projects.

Get in Touch
with GenScript CRISPR Experts

Get in Touch with GenScript CRISPR Experts
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