CThe GenCRISPR gRNA Design Tools makes guide RNA and HDR template design easy – Simply select your experiment type to get started.

Start Your Project

Note: Selecting HDR knock-in will enable HDR template design options

Easily design compatible
HDR templates & sgRNAs

Automatically optimize
homology arm design

Select from ssDNA
or dsDNA formats

Create an SNP or insert
any sequence up to 10 kb

Streamline your HDR
template & sgRNA orders

sgRNA/template pairs are
ranked by algorithm scores

Interactive Design Map

Paired sgRNA + HDR Templates

How to Design CRISPR Knock-in sgRNA + HDR Templates

Start Your Project

Note: Selecting HDR knock-in will enable HDR template design options

Other CRISPR gRNA Design Tool And Database

Broad gRNA Design Tool for Any Sequence

  • sgRNA designed for any DNA sequence you input
  • Algorithm developed and validated by Feng Zhang lab, Broad Institute

Broad sgRNA Database

  • Genome-wide gRNA databases for CRISPR editing and transcription activation
  • Pre-validated gRNA sequences design by the Broad Institute Ready for delivery; Full-Genome Coverage

GenCRISPR Services

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