The GenCRISPR gRNA Design Tools makes guide RNA and HDR template design easy – Simply select your experiment type to get started.

Start Your Project

Note: Selecting HDR knock-in will enable HDR template design options

Easily design compatible HDR templates & sgRNAs

Easily design compatible
HDR templates & sgRNAs

Automatically optimize
homology arm design

Select from ssDNA or dsDNA formats

Select from ssDNA
or dsDNA formats

Create an SNP or insert
any sequence up to 10 kb

Streamline your HDR template & sgRNA orders

Streamline your HDR
template & sgRNA orders

sgRNA/template pairs are
ranked by algorithm scores

Interactive Design Map

Interactive Design Map

Paired sgRNA + HDR Templates

Paired sgRNA + HDR Templates

How to Design CRISPR Knock-in sgRNA + HDR Templates

Start Your Project

Note: Selecting HDR knock-in will enable HDR template design options

Other CRISPR gRNA Design Tool And Database

Broad gRNA Design Tool for Any Sequence

  • sgRNA designed for any DNA sequence you input
  • Algorithm developed and validated by Feng Zhang lab, Broad Institute

Broad sgRNA Database

  • Genome-wide gRNA databases for CRISPR editing and transcription activation
  • Pre-validated gRNA sequences design by the Broad Institute Ready for delivery; Full-Genome Coverage

GenCRISPR Services

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