mlck-1 cDNA ORF clone, Caenorhabditis elegans

The following mlck-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mlck-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OCa350110 NM_077288.6
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Caenorhabditis elegans Protein kinase domain-containing protein (mlck-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
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OCa350110 NM_077288.4 Caenorhabditis elegans Myosin Light Chain Kinase related (mlck-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa350110
    Clone ID Related Accession (Same CDS sequence) NM_077288.4 , NM_077288.6
    Accession Version NM_077288.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Protein kinase domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_077288.5.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGACCG AACCCAGTGG AAGTGGAAAA ATCAAAGTTG ATGAACACTA TCCATCAGAA 
    CTAGATGAGC CTCCAGTGTT CGATGTGAAG AAAATTGAGA ACATTCGCGC AAATGTAAAG
    TTTGACACCC TTTATCAAGT AACCAAACTA TTAGGAGATG GAAAGTTTGG AAAAGTCTAT
    TGCGTGATAG AAAAAGAGAC CGGCAAAGAG TTTGCTGCAA AATTTATCAA AATCAGAAAA
    GAAGCCGATC GTGCGGAGGT GGAGAGAGAA GTGTCGATTC TCACGCAACT TCGCCATCCG
    AGAATCGCCC AAATTTACGA TGCATTTTAC ACCACAACCA ACGATGTGGT TTTAATTATG
    GAAATAGTTC GCGGTGGAGA GCTATTTGAT CGCGTCGCGG AAGAATCGTA TGTTTTGTCT
    GAACTTGCTG TCGTTATGAT CATCTGCCAA TTGTGTGAAG CAATTGACTA CATTCACAAG
    CAAAACATTC TTCATCTGGA TGTAAAGCCT GAAAACATTA TGTGTGTTTC TCTTACTGGT
    AATCGCATCA AACTCATTGA TTTTGGATTG GCTAGGCATT ACGATGGTAC ACAGGAGTTA
    AAATACATGG CGGGTACACC GGAATTCGCT GCTCCGGAAG TCATTAAATT TGAGAAATTG
    GATTATCACA CTGATATGTG GAGCATCGGA GTTATCACTT ATATTTTGCT TTCTGGATAC
    TCACCATTTC TTGGTGACAA CTTAGGAGAA ACATATTGCA ACGTTGAAAA GGGAGTCTGG
    GAGTTCACAG AAGAATTTGA CACTGTCACA GAAGAAGCGA AAGATTTTGT GACAAAGTTG
    TTAGTGTACG ATCAAAGCAA ACGTATGCTC CCACATGAAT GTCTTCAACA CCCATGGATA
    GCTAAACACC GTCAAAAAGC GGCGTGCAAC ACAATTCTTG AAAAACCTCT CAACGCTCCA
    ACACTTGACA ACAAGCAGAT TATGCGATAT AATGCACGAA GGAAATTCCG GCGTATGATA
    ATTTATGTAA AGTTCTTGAT TGAAATGAAT CGACTTCGGA ATTCCTTGAA AACTCGCATG
    AGTGCCAATG GACACAAATT CTTTGATCCA CTTTTGAAAA TGGCTGAGAA AAAAGAACAA
    AAGATTTCTA ACGCGATTGG CACAGCAGGT TGTCCTGCAA GTGGATTGGG ATCGCTTGTT
    AAAATGGCGG TGGACAGTCA AAAGAAGAAC GGAGACGTTC CAGTGACTCA AGACGCAAAT
    ATGTCCACAG AACCAGAGAA AGAAGAAAAG AAAAAGAAAA AGAAAATTGC CAATGCCGAT
    GGTTCTACAA AGACGGAGAA GACCTCTAGT GTGAAAAAGA AGAAATTAGT GGAGACAGAT
    GGTGATGCTG GACTTCGACC CCCACCGGAA CTAATGACAA AAAGAGCGTC AACTGGAGGG
    GAAAGTCTTT TGGCTGCAGT GAAGGTTCTT AAGAACTTGG AAGTGGTTGA AAATGGCAAT
    TCTCCGAAAA GGAAAAGTCT ATGTTCCGTT AAAGAAGAGA AAGAAATTCC AGCTAAAAAG
    CAAACAGAAG GCTATAAAAC ACTGGAAACT ACAGAAAATG GAGATATTAG GAGAAAGAGT
    GCACCAGTCA AACAAGACAC ATTGACGTGT CAAGACAACT CAAAAGTCAC TCCACAGTTG
    TTAGAAACTG CTCCCAATGG AGAGATCAGA AGGAAAAGCG CTTCGGCAAA AGAAGAAAAA
    TCCGTCAAGC CAGAAGAAGT TATTCGTAAG AAAAGTTCCG CGTCTGTCAA AGAAACAAAA
    ACAGTGGAAC TTTCTGTAGT TCCCGTAAAA CTGGAAAAGT TAGCATCAAT TGATGAAAAA
    GGAGAAAAAC TTGTGAAAAA AAAGAAGAAA ACTACCACAG TTACGGCGGA GATAGAAAAA
    CCAAAATCAG TAATAGAGTC AAAAGAAGAA AAACTTGTGG AAAAACCAAA GCTGTTGAAT
    GGAACTTTAG TTTCTGATGC TCTTGAAAAA CGTAGAAGTT TGGGCTCAAA GCGGGCAAGC
    GAAACTCAAG ACGTCGGATT AAGACCTCTT CCGGTAGTCG ACTTCTCTCC GAAAATCGTA
    CTCACTGATA AGAAAGAGCC GAAGGAGTCA AAAACCAAAC CTGCCGAACC ATTAAAAGAA
    ATTGTTCCCG AAACAAAAAA ACCAGCAAGA CAGGGCGCTC TTCGTAAACG AGACAAGTCA
    CTAGATGATA TCCTCAATGC CGCTCCGTCC CCAACCACCT TGTCAAAATC AACTGTGACA
    TCTGTAAAGC ATTCAGTTGT GGAAACAATT CATCCGAGCG GGGAAATAAT CAAATCGGAA
    AAGGTTCTCA CAAAGAAAAA GAGTTTGAAA AAAGATAAAA GTATCGATGA TCGTCTTCCT
    GTGAAGATTA TCGAGGACAA AGCTAGTCTT CCGAAGTTGA AAACGGATGT GGTGACTTTC
    GAATCGAAGC CAGTCAAGAA AACTTCAAAG ATCGTCAAGC TCATTCCAGA AGATGCAAAT
    TTAATAAAAG ACGAGAAGAA ACCAACAAAG ATTGTGATGA CGTCAGAAAA GCAGGCGACA
    ACAACTGTTC AAACTACAAC AAGTGTCAAA CGGACAACAA AGAGTTCTGA AAAGAAAGTG
    GAACATACGG CTGATGGAAA GAGTGTAGAG TCGTCCCAGA AGAAAAATTC ACAAAAAGAT
    GACGTCAAAG TTAGCCAGGT AACCACTAAA AAGGAAGAAG ACAGTACTCA GCCAGCACCT
    ACACTTACCG TTAAGAAAAA TGTTGTGAAA CAAACTGCTG AAAAGTCGAC ATCTGAGAAA
    GAGCACAAAA CAACAGAAAA CAAAGAGCAA GAGAGGATAA TCAAAAAGAG CAGTACTCTA
    AAAGACAAAG ATGTTGCACA AGTTTCACTT CAACAAAGTA TCAAGGTTAA TGGTGAAAAG
    CACAAGGAGT CAAAAGTTAT TGGCACTAAA ACTGTTATAA ATAAGTTAGA ATTGAAAGGT
    GAAGAAACCA TTAATGCCAA TCCCAAGAAA TCATCACTCA AAAAGGCAAC AGCCAAAGTC
    ACAGGAGAGA CAACAAAACA CCTCGAAATT TCTACGGATG GGAAACTAGG TGTTCTCAAA
    ACAAAAGACA CAACTTCGAT GAAATTATCT CCGGACTCAA GCAGCGATAC ATCGTCAAGC
    AAAAATGTGC GGATCCTTTT AACAGATGAA CAGTCAGTTA AGTCGAGTGT AAGATCTCAT
    CCTAGTGTTA GCACCAGAGA TAGTTCAGAG GAAAGGAAGA AGGTTCGCTT TGCCGGTGAT
    GTTGGGACAC CGACGAAACT AAATCCCCCT CCACTTTTTG GGAATGGTCT TCAGAAAATG
    AGATCTGAAA GTAGTTTACA CGAAAAAATC AACGGACTCA CTACCAAAAA GAGTCATGAT
    GACGTGTCGC TCGCTAGAAA AACATCACTG TTCACAGATA TTGAATACCA ACCACGCGAA
    GATTTTTCGT TTGAAGCATT GAAAGAAAAA TTGACACGAC GGGTCAGCTA TGGATATGAC
    GATGATGGCA AACGTGGAAA ACAAATGGTA CTAATTCCAC CTACAAATTC AGTTAAAGAC
    AAGCTCCGAC ATTTTGAGAA GAAGTTTGCC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_509689.1
    CDS126..3761
    Translation

    Target ORF information:

    RefSeq Version NM_077288.6
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Protein kinase domain-containing protein (mlck-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_077288.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGACCG AACCCAGTGG AAGTGGAAAA ATCAAAGTTG ATGAACACTA TCCATCAGAA 
    CTAGATGAGC CTCCAGTGTT CGATGTGAAG AAAATTGAGA ACATTCGCGC AAATGTAAAG
    TTTGACACCC TTTATCAAGT AACCAAACTA TTAGGAGATG GAAAGTTTGG AAAAGTCTAT
    TGCGTGATAG AAAAAGAGAC CGGCAAAGAG TTTGCTGCAA AATTTATCAA AATCAGAAAA
    GAAGCCGATC GTGCGGAGGT GGAGAGAGAA GTGTCGATTC TCACGCAACT TCGCCATCCG
    AGAATCGCCC AAATTTACGA TGCATTTTAC ACCACAACCA ACGATGTGGT TTTAATTATG
    GAAATAGTTC GCGGTGGAGA GCTATTTGAT CGCGTCGCGG AAGAATCGTA TGTTTTGTCT
    GAACTTGCTG TCGTTATGAT CATCTGCCAA TTGTGTGAAG CAATTGACTA CATTCACAAG
    CAAAACATTC TTCATCTGGA TGTAAAGCCT GAAAACATTA TGTGTGTTTC TCTTACTGGT
    AATCGCATCA AACTCATTGA TTTTGGATTG GCTAGGCATT ACGATGGTAC ACAGGAGTTA
    AAATACATGG CGGGTACACC GGAATTCGCT GCTCCGGAAG TCATTAAATT TGAGAAATTG
    GATTATCACA CTGATATGTG GAGCATCGGA GTTATCACTT ATATTTTGCT TTCTGGATAC
    TCACCATTTC TTGGTGACAA CTTAGGAGAA ACATATTGCA ACGTTGAAAA GGGAGTCTGG
    GAGTTCACAG AAGAATTTGA CACTGTCACA GAAGAAGCGA AAGATTTTGT GACAAAGTTG
    TTAGTGTACG ATCAAAGCAA ACGTATGCTC CCACATGAAT GTCTTCAACA CCCATGGATA
    GCTAAACACC GTCAAAAAGC GGCGTGCAAC ACAATTCTTG AAAAACCTCT CAACGCTCCA
    ACACTTGACA ACAAGCAGAT TATGCGATAT AATGCACGAA GGAAATTCCG GCGTATGATA
    ATTTATGTAA AGTTCTTGAT TGAAATGAAT CGACTTCGGA ATTCCTTGAA AACTCGCATG
    AGTGCCAATG GACACAAATT CTTTGATCCA CTTTTGAAAA TGGCTGAGAA AAAAGAACAA
    AAGATTTCTA ACGCGATTGG CACAGCAGGT TGTCCTGCAA GTGGATTGGG ATCGCTTGTT
    AAAATGGCGG TGGACAGTCA AAAGAAGAAC GGAGACGTTC CAGTGACTCA AGACGCAAAT
    ATGTCCACAG AACCAGAGAA AGAAGAAAAG AAAAAGAAAA AGAAAATTGC CAATGCCGAT
    GGTTCTACAA AGACGGAGAA GACCTCTAGT GTGAAAAAGA AGAAATTAGT GGAGACAGAT
    GGTGATGCTG GACTTCGACC CCCACCGGAA CTAATGACAA AAAGAGCGTC AACTGGAGGG
    GAAAGTCTTT TGGCTGCAGT GAAGGTTCTT AAGAACTTGG AAGTGGTTGA AAATGGCAAT
    TCTCCGAAAA GGAAAAGTCT ATGTTCCGTT AAAGAAGAGA AAGAAATTCC AGCTAAAAAG
    CAAACAGAAG GCTATAAAAC ACTGGAAACT ACAGAAAATG GAGATATTAG GAGAAAGAGT
    GCACCAGTCA AACAAGACAC ATTGACGTGT CAAGACAACT CAAAAGTCAC TCCACAGTTG
    TTAGAAACTG CTCCCAATGG AGAGATCAGA AGGAAAAGCG CTTCGGCAAA AGAAGAAAAA
    TCCGTCAAGC CAGAAGAAGT TATTCGTAAG AAAAGTTCCG CGTCTGTCAA AGAAACAAAA
    ACAGTGGAAC TTTCTGTAGT TCCCGTAAAA CTGGAAAAGT TAGCATCAAT TGATGAAAAA
    GGAGAAAAAC TTGTGAAAAA AAAGAAGAAA ACTACCACAG TTACGGCGGA GATAGAAAAA
    CCAAAATCAG TAATAGAGTC AAAAGAAGAA AAACTTGTGG AAAAACCAAA GCTGTTGAAT
    GGAACTTTAG TTTCTGATGC TCTTGAAAAA CGTAGAAGTT TGGGCTCAAA GCGGGCAAGC
    GAAACTCAAG ACGTCGGATT AAGACCTCTT CCGGTAGTCG ACTTCTCTCC GAAAATCGTA
    CTCACTGATA AGAAAGAGCC GAAGGAGTCA AAAACCAAAC CTGCCGAACC ATTAAAAGAA
    ATTGTTCCCG AAACAAAAAA ACCAGCAAGA CAGGGCGCTC TTCGTAAACG AGACAAGTCA
    CTAGATGATA TCCTCAATGC CGCTCCGTCC CCAACCACCT TGTCAAAATC AACTGTGACA
    TCTGTAAAGC ATTCAGTTGT GGAAACAATT CATCCGAGCG GGGAAATAAT CAAATCGGAA
    AAGGTTCTCA CAAAGAAAAA GAGTTTGAAA AAAGATAAAA GTATCGATGA TCGTCTTCCT
    GTGAAGATTA TCGAGGACAA AGCTAGTCTT CCGAAGTTGA AAACGGATGT GGTGACTTTC
    GAATCGAAGC CAGTCAAGAA AACTTCAAAG ATCGTCAAGC TCATTCCAGA AGATGCAAAT
    TTAATAAAAG ACGAGAAGAA ACCAACAAAG ATTGTGATGA CGTCAGAAAA GCAGGCGACA
    ACAACTGTTC AAACTACAAC AAGTGTCAAA CGGACAACAA AGAGTTCTGA AAAGAAAGTG
    GAACATACGG CTGATGGAAA GAGTGTAGAG TCGTCCCAGA AGAAAAATTC ACAAAAAGAT
    GACGTCAAAG TTAGCCAGGT AACCACTAAA AAGGAAGAAG ACAGTACTCA GCCAGCACCT
    ACACTTACCG TTAAGAAAAA TGTTGTGAAA CAAACTGCTG AAAAGTCGAC ATCTGAGAAA
    GAGCACAAAA CAACAGAAAA CAAAGAGCAA GAGAGGATAA TCAAAAAGAG CAGTACTCTA
    AAAGACAAAG ATGTTGCACA AGTTTCACTT CAACAAAGTA TCAAGGTTAA TGGTGAAAAG
    CACAAGGAGT CAAAAGTTAT TGGCACTAAA ACTGTTATAA ATAAGTTAGA ATTGAAAGGT
    GAAGAAACCA TTAATGCCAA TCCCAAGAAA TCATCACTCA AAAAGGCAAC AGCCAAAGTC
    ACAGGAGAGA CAACAAAACA CCTCGAAATT TCTACGGATG GGAAACTAGG TGTTCTCAAA
    ACAAAAGACA CAACTTCGAT GAAATTATCT CCGGACTCAA GCAGCGATAC ATCGTCAAGC
    AAAAATGTGC GGATCCTTTT AACAGATGAA CAGTCAGTTA AGTCGAGTGT AAGATCTCAT
    CCTAGTGTTA GCACCAGAGA TAGTTCAGAG GAAAGGAAGA AGGTTCGCTT TGCCGGTGAT
    GTTGGGACAC CGACGAAACT AAATCCCCCT CCACTTTTTG GGAATGGTCT TCAGAAAATG
    AGATCTGAAA GTAGTTTACA CGAAAAAATC AACGGACTCA CTACCAAAAA GAGTCATGAT
    GACGTGTCGC TCGCTAGAAA AACATCACTG TTCACAGATA TTGAATACCA ACCACGCGAA
    GATTTTTCGT TTGAAGCATT GAAAGAAAAA TTGACACGAC GGGTCAGCTA TGGATATGAC
    GATGATGGCA AACGTGGAAA ACAAATGGTA CTAATTCCAC CTACAAATTC AGTTAAAGAC
    AAGCTCCGAC ATTTTGAGAA GAAGTTTGCC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa350110
    Clone ID Related Accession (Same CDS sequence) NM_077288.4 , NM_077288.6
    Accession Version NM_077288.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Myosin Light Chain Kinase related
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_077288.3. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGACCG AACCCAGTGG AAGTGGAAAA ATCAAAGTTG ATGAACACTA TCCATCAGAA 
    CTAGATGAGC CTCCAGTGTT CGATGTGAAG AAAATTGAGA ACATTCGCGC AAATGTAAAG
    TTTGACACCC TTTATCAAGT AACCAAACTA TTAGGAGATG GAAAGTTTGG AAAAGTCTAT
    TGCGTGATAG AAAAAGAGAC CGGCAAAGAG TTTGCTGCAA AATTTATCAA AATCAGAAAA
    GAAGCCGATC GTGCGGAGGT GGAGAGAGAA GTGTCGATTC TCACGCAACT TCGCCATCCG
    AGAATCGCCC AAATTTACGA TGCATTTTAC ACCACAACCA ACGATGTGGT TTTAATTATG
    GAAATAGTTC GCGGTGGAGA GCTATTTGAT CGCGTCGCGG AAGAATCGTA TGTTTTGTCT
    GAACTTGCTG TCGTTATGAT CATCTGCCAA TTGTGTGAAG CAATTGACTA CATTCACAAG
    CAAAACATTC TTCATCTGGA TGTAAAGCCT GAAAACATTA TGTGTGTTTC TCTTACTGGT
    AATCGCATCA AACTCATTGA TTTTGGATTG GCTAGGCATT ACGATGGTAC ACAGGAGTTA
    AAATACATGG CGGGTACACC GGAATTCGCT GCTCCGGAAG TCATTAAATT TGAGAAATTG
    GATTATCACA CTGATATGTG GAGCATCGGA GTTATCACTT ATATTTTGCT TTCTGGATAC
    TCACCATTTC TTGGTGACAA CTTAGGAGAA ACATATTGCA ACGTTGAAAA GGGAGTCTGG
    GAGTTCACAG AAGAATTTGA CACTGTCACA GAAGAAGCGA AAGATTTTGT GACAAAGTTG
    TTAGTGTACG ATCAAAGCAA ACGTATGCTC CCACATGAAT GTCTTCAACA CCCATGGATA
    GCTAAACACC GTCAAAAAGC GGCGTGCAAC ACAATTCTTG AAAAACCTCT CAACGCTCCA
    ACACTTGACA ACAAGCAGAT TATGCGATAT AATGCACGAA GGAAATTCCG GCGTATGATA
    ATTTATGTAA AGTTCTTGAT TGAAATGAAT CGACTTCGGA ATTCCTTGAA AACTCGCATG
    AGTGCCAATG GACACAAATT CTTTGATCCA CTTTTGAAAA TGGCTGAGAA AAAAGAACAA
    AAGATTTCTA ACGCGATTGG CACAGCAGGT TGTCCTGCAA GTGGATTGGG ATCGCTTGTT
    AAAATGGCGG TGGACAGTCA AAAGAAGAAC GGAGACGTTC CAGTGACTCA AGACGCAAAT
    ATGTCCACAG AACCAGAGAA AGAAGAAAAG AAAAAGAAAA AGAAAATTGC CAATGCCGAT
    GGTTCTACAA AGACGGAGAA GACCTCTAGT GTGAAAAAGA AGAAATTAGT GGAGACAGAT
    GGTGATGCTG GACTTCGACC CCCACCGGAA CTAATGACAA AAAGAGCGTC AACTGGAGGG
    GAAAGTCTTT TGGCTGCAGT GAAGGTTCTT AAGAACTTGG AAGTGGTTGA AAATGGCAAT
    TCTCCGAAAA GGAAAAGTCT ATGTTCCGTT AAAGAAGAGA AAGAAATTCC AGCTAAAAAG
    CAAACAGAAG GCTATAAAAC ACTGGAAACT ACAGAAAATG GAGATATTAG GAGAAAGAGT
    GCACCAGTCA AACAAGACAC ATTGACGTGT CAAGACAACT CAAAAGTCAC TCCACAGTTG
    TTAGAAACTG CTCCCAATGG AGAGATCAGA AGGAAAAGCG CTTCGGCAAA AGAAGAAAAA
    TCCGTCAAGC CAGAAGAAGT TATTCGTAAG AAAAGTTCCG CGTCTGTCAA AGAAACAAAA
    ACAGTGGAAC TTTCTGTAGT TCCCGTAAAA CTGGAAAAGT TAGCATCAAT TGATGAAAAA
    GGAGAAAAAC TTGTGAAAAA AAAGAAGAAA ACTACCACAG TTACGGCGGA GATAGAAAAA
    CCAAAATCAG TAATAGAGTC AAAAGAAGAA AAACTTGTGG AAAAACCAAA GCTGTTGAAT
    GGAACTTTAG TTTCTGATGC TCTTGAAAAA CGTAGAAGTT TGGGCTCAAA GCGGGCAAGC
    GAAACTCAAG ACGTCGGATT AAGACCTCTT CCGGTAGTCG ACTTCTCTCC GAAAATCGTA
    CTCACTGATA AGAAAGAGCC GAAGGAGTCA AAAACCAAAC CTGCCGAACC ATTAAAAGAA
    ATTGTTCCCG AAACAAAAAA ACCAGCAAGA CAGGGCGCTC TTCGTAAACG AGACAAGTCA
    CTAGATGATA TCCTCAATGC CGCTCCGTCC CCAACCACCT TGTCAAAATC AACTGTGACA
    TCTGTAAAGC ATTCAGTTGT GGAAACAATT CATCCGAGCG GGGAAATAAT CAAATCGGAA
    AAGGTTCTCA CAAAGAAAAA GAGTTTGAAA AAAGATAAAA GTATCGATGA TCGTCTTCCT
    GTGAAGATTA TCGAGGACAA AGCTAGTCTT CCGAAGTTGA AAACGGATGT GGTGACTTTC
    GAATCGAAGC CAGTCAAGAA AACTTCAAAG ATCGTCAAGC TCATTCCAGA AGATGCAAAT
    TTAATAAAAG ACGAGAAGAA ACCAACAAAG ATTGTGATGA CGTCAGAAAA GCAGGCGACA
    ACAACTGTTC AAACTACAAC AAGTGTCAAA CGGACAACAA AGAGTTCTGA AAAGAAAGTG
    GAACATACGG CTGATGGAAA GAGTGTAGAG TCGTCCCAGA AGAAAAATTC ACAAAAAGAT
    GACGTCAAAG TTAGCCAGGT AACCACTAAA AAGGAAGAAG ACAGTACTCA GCCAGCACCT
    ACACTTACCG TTAAGAAAAA TGTTGTGAAA CAAACTGCTG AAAAGTCGAC ATCTGAGAAA
    GAGCACAAAA CAACAGAAAA CAAAGAGCAA GAGAGGATAA TCAAAAAGAG CAGTACTCTA
    AAAGACAAAG ATGTTGCACA AGTTTCACTT CAACAAAGTA TCAAGGTTAA TGGTGAAAAG
    CACAAGGAGT CAAAAGTTAT TGGCACTAAA ACTGTTATAA ATAAGTTAGA ATTGAAAGGT
    GAAGAAACCA TTAATGCCAA TCCCAAGAAA TCATCACTCA AAAAGGCAAC AGCCAAAGTC
    ACAGGAGAGA CAACAAAACA CCTCGAAATT TCTACGGATG GGAAACTAGG TGTTCTCAAA
    ACAAAAGACA CAACTTCGAT GAAATTATCT CCGGACTCAA GCAGCGATAC ATCGTCAAGC
    AAAAATGTGC GGATCCTTTT AACAGATGAA CAGTCAGTTA AGTCGAGTGT AAGATCTCAT
    CCTAGTGTTA GCACCAGAGA TAGTTCAGAG GAAAGGAAGA AGGTTCGCTT TGCCGGTGAT
    GTTGGGACAC CGACGAAACT AAATCCCCCT CCACTTTTTG GGAATGGTCT TCAGAAAATG
    AGATCTGAAA GTAGTTTACA CGAAAAAATC AACGGACTCA CTACCAAAAA GAGTCATGAT
    GACGTGTCGC TCGCTAGAAA AACATCACTG TTCACAGATA TTGAATACCA ACCACGCGAA
    GATTTTTCGT TTGAAGCATT GAAAGAAAAA TTGACACGAC GGGTCAGCTA TGGATATGAC
    GATGATGGCA AACGTGGAAA ACAAATGGTA CTAATTCCAC CTACAAATTC AGTTAAAGAC
    AAGCTCCGAC ATTTTGAGAA GAAGTTTGCC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_509689.1
    CDS1..3636
    Translation

    Target ORF information:

    RefSeq Version NM_077288.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Myosin Light Chain Kinase related (mlck-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_077288.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGACCG AACCCAGTGG AAGTGGAAAA ATCAAAGTTG ATGAACACTA TCCATCAGAA 
    CTAGATGAGC CTCCAGTGTT CGATGTGAAG AAAATTGAGA ACATTCGCGC AAATGTAAAG
    TTTGACACCC TTTATCAAGT AACCAAACTA TTAGGAGATG GAAAGTTTGG AAAAGTCTAT
    TGCGTGATAG AAAAAGAGAC CGGCAAAGAG TTTGCTGCAA AATTTATCAA AATCAGAAAA
    GAAGCCGATC GTGCGGAGGT GGAGAGAGAA GTGTCGATTC TCACGCAACT TCGCCATCCG
    AGAATCGCCC AAATTTACGA TGCATTTTAC ACCACAACCA ACGATGTGGT TTTAATTATG
    GAAATAGTTC GCGGTGGAGA GCTATTTGAT CGCGTCGCGG AAGAATCGTA TGTTTTGTCT
    GAACTTGCTG TCGTTATGAT CATCTGCCAA TTGTGTGAAG CAATTGACTA CATTCACAAG
    CAAAACATTC TTCATCTGGA TGTAAAGCCT GAAAACATTA TGTGTGTTTC TCTTACTGGT
    AATCGCATCA AACTCATTGA TTTTGGATTG GCTAGGCATT ACGATGGTAC ACAGGAGTTA
    AAATACATGG CGGGTACACC GGAATTCGCT GCTCCGGAAG TCATTAAATT TGAGAAATTG
    GATTATCACA CTGATATGTG GAGCATCGGA GTTATCACTT ATATTTTGCT TTCTGGATAC
    TCACCATTTC TTGGTGACAA CTTAGGAGAA ACATATTGCA ACGTTGAAAA GGGAGTCTGG
    GAGTTCACAG AAGAATTTGA CACTGTCACA GAAGAAGCGA AAGATTTTGT GACAAAGTTG
    TTAGTGTACG ATCAAAGCAA ACGTATGCTC CCACATGAAT GTCTTCAACA CCCATGGATA
    GCTAAACACC GTCAAAAAGC GGCGTGCAAC ACAATTCTTG AAAAACCTCT CAACGCTCCA
    ACACTTGACA ACAAGCAGAT TATGCGATAT AATGCACGAA GGAAATTCCG GCGTATGATA
    ATTTATGTAA AGTTCTTGAT TGAAATGAAT CGACTTCGGA ATTCCTTGAA AACTCGCATG
    AGTGCCAATG GACACAAATT CTTTGATCCA CTTTTGAAAA TGGCTGAGAA AAAAGAACAA
    AAGATTTCTA ACGCGATTGG CACAGCAGGT TGTCCTGCAA GTGGATTGGG ATCGCTTGTT
    AAAATGGCGG TGGACAGTCA AAAGAAGAAC GGAGACGTTC CAGTGACTCA AGACGCAAAT
    ATGTCCACAG AACCAGAGAA AGAAGAAAAG AAAAAGAAAA AGAAAATTGC CAATGCCGAT
    GGTTCTACAA AGACGGAGAA GACCTCTAGT GTGAAAAAGA AGAAATTAGT GGAGACAGAT
    GGTGATGCTG GACTTCGACC CCCACCGGAA CTAATGACAA AAAGAGCGTC AACTGGAGGG
    GAAAGTCTTT TGGCTGCAGT GAAGGTTCTT AAGAACTTGG AAGTGGTTGA AAATGGCAAT
    TCTCCGAAAA GGAAAAGTCT ATGTTCCGTT AAAGAAGAGA AAGAAATTCC AGCTAAAAAG
    CAAACAGAAG GCTATAAAAC ACTGGAAACT ACAGAAAATG GAGATATTAG GAGAAAGAGT
    GCACCAGTCA AACAAGACAC ATTGACGTGT CAAGACAACT CAAAAGTCAC TCCACAGTTG
    TTAGAAACTG CTCCCAATGG AGAGATCAGA AGGAAAAGCG CTTCGGCAAA AGAAGAAAAA
    TCCGTCAAGC CAGAAGAAGT TATTCGTAAG AAAAGTTCCG CGTCTGTCAA AGAAACAAAA
    ACAGTGGAAC TTTCTGTAGT TCCCGTAAAA CTGGAAAAGT TAGCATCAAT TGATGAAAAA
    GGAGAAAAAC TTGTGAAAAA AAAGAAGAAA ACTACCACAG TTACGGCGGA GATAGAAAAA
    CCAAAATCAG TAATAGAGTC AAAAGAAGAA AAACTTGTGG AAAAACCAAA GCTGTTGAAT
    GGAACTTTAG TTTCTGATGC TCTTGAAAAA CGTAGAAGTT TGGGCTCAAA GCGGGCAAGC
    GAAACTCAAG ACGTCGGATT AAGACCTCTT CCGGTAGTCG ACTTCTCTCC GAAAATCGTA
    CTCACTGATA AGAAAGAGCC GAAGGAGTCA AAAACCAAAC CTGCCGAACC ATTAAAAGAA
    ATTGTTCCCG AAACAAAAAA ACCAGCAAGA CAGGGCGCTC TTCGTAAACG AGACAAGTCA
    CTAGATGATA TCCTCAATGC CGCTCCGTCC CCAACCACCT TGTCAAAATC AACTGTGACA
    TCTGTAAAGC ATTCAGTTGT GGAAACAATT CATCCGAGCG GGGAAATAAT CAAATCGGAA
    AAGGTTCTCA CAAAGAAAAA GAGTTTGAAA AAAGATAAAA GTATCGATGA TCGTCTTCCT
    GTGAAGATTA TCGAGGACAA AGCTAGTCTT CCGAAGTTGA AAACGGATGT GGTGACTTTC
    GAATCGAAGC CAGTCAAGAA AACTTCAAAG ATCGTCAAGC TCATTCCAGA AGATGCAAAT
    TTAATAAAAG ACGAGAAGAA ACCAACAAAG ATTGTGATGA CGTCAGAAAA GCAGGCGACA
    ACAACTGTTC AAACTACAAC AAGTGTCAAA CGGACAACAA AGAGTTCTGA AAAGAAAGTG
    GAACATACGG CTGATGGAAA GAGTGTAGAG TCGTCCCAGA AGAAAAATTC ACAAAAAGAT
    GACGTCAAAG TTAGCCAGGT AACCACTAAA AAGGAAGAAG ACAGTACTCA GCCAGCACCT
    ACACTTACCG TTAAGAAAAA TGTTGTGAAA CAAACTGCTG AAAAGTCGAC ATCTGAGAAA
    GAGCACAAAA CAACAGAAAA CAAAGAGCAA GAGAGGATAA TCAAAAAGAG CAGTACTCTA
    AAAGACAAAG ATGTTGCACA AGTTTCACTT CAACAAAGTA TCAAGGTTAA TGGTGAAAAG
    CACAAGGAGT CAAAAGTTAT TGGCACTAAA ACTGTTATAA ATAAGTTAGA ATTGAAAGGT
    GAAGAAACCA TTAATGCCAA TCCCAAGAAA TCATCACTCA AAAAGGCAAC AGCCAAAGTC
    ACAGGAGAGA CAACAAAACA CCTCGAAATT TCTACGGATG GGAAACTAGG TGTTCTCAAA
    ACAAAAGACA CAACTTCGAT GAAATTATCT CCGGACTCAA GCAGCGATAC ATCGTCAAGC
    AAAAATGTGC GGATCCTTTT AACAGATGAA CAGTCAGTTA AGTCGAGTGT AAGATCTCAT
    CCTAGTGTTA GCACCAGAGA TAGTTCAGAG GAAAGGAAGA AGGTTCGCTT TGCCGGTGAT
    GTTGGGACAC CGACGAAACT AAATCCCCCT CCACTTTTTG GGAATGGTCT TCAGAAAATG
    AGATCTGAAA GTAGTTTACA CGAAAAAATC AACGGACTCA CTACCAAAAA GAGTCATGAT
    GACGTGTCGC TCGCTAGAAA AACATCACTG TTCACAGATA TTGAATACCA ACCACGCGAA
    GATTTTTCGT TTGAAGCATT GAAAGAAAAA TTGACACGAC GGGTCAGCTA TGGATATGAC
    GATGATGGCA AACGTGGAAA ACAAATGGTA CTAATTCCAC CTACAAATTC AGTTAAAGAC
    AAGCTCCGAC ATTTTGAGAA GAAGTTTGCC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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