ADGRB3 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Gene Symbol	ADGRB3
Entrez Gene ID	481870
Full Name	adhesion G protein-coupled receptor B3
Synonyms	BAI3
General protein information	Preferred Names adhesion G protein-coupled receptor B3 Names adhesion G protein-coupled receptor B3 brain-specific angiogenesis inhibitor 3
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005627521.3	XP_005627578.1	adhesion G protein-coupled receptor B3 isoform X3
XM_005627521.2	XP_005627578.1	brain-specific angiogenesis inhibitor 3 isoform X3
XM_538991.5	XP_538991.2	brain-specific angiogenesis inhibitor 3 isoform X1
XM_538991.6	XP_538991.2	adhesion G protein-coupled receptor B3 isoform X1
XM_861971.5	XP_867064.1	adhesion G protein-coupled receptor B3 isoform X1
XM_014118101.2	XP_013973576.1	adhesion G protein-coupled receptor B3 isoform X2
XM_861971.4	XP_867064.1	brain-specific angiogenesis inhibitor 3 isoform X1
XM_014118101.1	XP_013973576.1	brain-specific angiogenesis inhibitor 3 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BAI3	XP_867064.1
Bos taurus (cattle)	BAI3	XP_002690045.3
	BAI3	XP_002803849.1
Mus musculus (house mouse)	Bai3	NP_783573.4
Rattus norvegicus (Norway rat)	Bai3	NP_001100368.1
Danio rerio (zebrafish)	bai3	XP_003199786.1
Homo sapiens (human)	BAI3	NP_001695.1
Pan troglodytes (chimpanzee)	BAI3	XP_003311446.1
Gallus gallus (chicken)	BAI3	XP_419892.3
Xenopus tropicalis (tropical clawed frog)	bai3	XP_002936367.2

Gene Ontology

The following ADGRB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe39372	XM_014118101.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe15460	XM_861971.5 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe15460	XM_538991.6 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe15461	XM_005627521.3 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe15461	XM_005627521.2	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe15460	XM_538991.5	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe15460	XM_861971.4	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe39372	XM_014118101.1	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADGRB3 ( XM_014118101.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013973576.1 Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe39372
Clone ID Related Accession (Same CDS sequence)	XM_014118101.2 , XM_014118101.1
Accession Version	XM_014118101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAAGGTCAT GCATACAAGG 
AAGAGGCATA TGGAACTGTT TCAGGAGCTG AATCAGAAGT TTCAAACTTT GGACAGATTT 
CGGGATATAC CAAATACAAG TAGTATGGAA AACCCAGCAC CAAACAAGAA TCCATGGGAC 
ACTTTCAAAA ACCCCAGTGA ATACCAACAT TATACCACAA TCAATGTCTT AGATACAGAG 
GCAAAGGATG CTTTGGAACT GAGGCCAGCA GAGTGGGAGA AGTGTCTGAA TTTGCCTCTG 
GATGTGCAAG AGGGTGACTT TCAAACAGAA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973576.1
CDS	841..5376
Translation	MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCDSKNAFVFLQYDKNFIQIRRVFPTDFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLVLLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSPWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRMRTCQGAAVTGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSITEHLAKGQRVLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRANYTPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGIGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSSKELDESLVFVLGAVLYKNLDLILPTLRNYTVVNSKVIVVTIRPEPKTSDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHSGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGMHMPMSMNELGNQCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNTQPSMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLDQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSRSETGSTISMSSLEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYQHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV

Target ORF information:

RefSeq Version	XM_014118101.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014118101.2

ORF Insert Sequence:

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAAGGTCAT GCATACAAGG 
AAGAGGCATA TGGAACTGTT TCAGGAGCTG AATCAGAAGT TTCAAACTTT GGACAGATTT 
CGGGATATAC CAAATACAAG TAGTATGGAA AACCCAGCAC CAAACAAGAA TCCATGGGAC 
ACTTTCAAAA ACCCCAGTGA ATACCAACAT TATACCACAA TCAATGTCTT AGATACAGAG 
GCAAAGGATG CTTTGGAACT GAGGCCAGCA GAGTGGGAGA AGTGTCTGAA TTTGCCTCTG 
GATGTGCAAG AGGGTGACTT TCAAACAGAA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB3 ( XM_014118101.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013973576.1 Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe39372
Clone ID Related Accession (Same CDS sequence)	XM_014118101.2 , XM_014118101.1
Accession Version	XM_014118101.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	brain-specific angiogenesis inhibitor 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAAGGTCAT GCATACAAGG 
AAGAGGCATA TGGAACTGTT TCAGGAGCTG AATCAGAAGT TTCAAACTTT GGACAGATTT 
CGGGATATAC CAAATACAAG TAGTATGGAA AACCCAGCAC CAAACAAGAA TCCATGGGAC 
ACTTTCAAAA ACCCCAGTGA ATACCAACAT TATACCACAA TCAATGTCTT AGATACAGAG 
GCAAAGGATG CTTTGGAACT GAGGCCAGCA GAGTGGGAGA AGTGTCTGAA TTTGCCTCTG 
GATGTGCAAG AGGGTGACTT TCAAACAGAA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973576.1
CDS	825..5360
Translation	MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCDSKNAFVFLQYDKNFIQIRRVFPTDFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLVLLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSPWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRMRTCQGAAVTGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSITEHLAKGQRVLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRANYTPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGIGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSSKELDESLVFVLGAVLYKNLDLILPTLRNYTVVNSKVIVVTIRPEPKTSDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHSGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGMHMPMSMNELGNQCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNTQPSMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLDQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSRSETGSTISMSSLEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYQHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV

Target ORF information:

RefSeq Version	XM_014118101.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014118101.1

ORF Insert Sequence:

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAAGGTCAT GCATACAAGG 
AAGAGGCATA TGGAACTGTT TCAGGAGCTG AATCAGAAGT TTCAAACTTT GGACAGATTT 
CGGGATATAC CAAATACAAG TAGTATGGAA AACCCAGCAC CAAACAAGAA TCCATGGGAC 
ACTTTCAAAA ACCCCAGTGA ATACCAACAT TATACCACAA TCAATGTCTT AGATACAGAG 
GCAAAGGATG CTTTGGAACT GAGGCCAGCA GAGTGGGAGA AGTGTCTGAA TTTGCCTCTG 
GATGTGCAAG AGGGTGACTT TCAAACAGAA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB3 ( XM_538991.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_538991.2 Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X1, mRNA.

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CloneID	OCe15460
Clone ID Related Accession (Same CDS sequence)	XM_538991.5 , XM_538991.6 , XM_861971.5 , XM_861971.4
Accession Version	XM_538991.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	brain-specific angiogenesis inhibitor 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_538991.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAGAAGAAA ATCACGATAT 
TCAGACCTTG ACTTTGAGAA GGTCATGCAT ACAAGGAAGA GGCATATGGA ACTGTTTCAG 
GAGCTGAATC AGAAGTTTCA AACTTTGGAC AGATTTCGGG ATATACCAAA TACAAGTAGT 
ATGGAAAACC CAGCACCAAA CAAGAATCCA TGGGACACTT TCAAAAACCC CAGTGAATAC 
CAACATTATA CCACAATCAA TGTCTTAGAT ACAGAGGCAA AGGATGCTTT GGAACTGAGG 
CCAGCAGAGT GGGAGAAGTG TCTGAATTTG CCTCTGGATG TGCAAGAGGG TGACTTTCAA 
ACAGAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_538991.2
CDS	824..5395
Translation	MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCDSKNAFVFLQYDKNFIQIRRVFPTDFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLVLLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSPWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRMRTCQGAAVTGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSITEHLAKGQRVLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRANYTPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGIGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSSKELDESLVFVLGAVLYKNLDLILPTLRNYTVVNSKVIVVTIRPEPKTSDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHSGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGMHMPMSMNELGNQCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNTQPSMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLDQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSRSETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYQHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV

Target ORF information:

RefSeq Version	XM_538991.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_538991.5

ORF Insert Sequence:

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAGAAGAAA ATCACGATAT 
TCAGACCTTG ACTTTGAGAA GGTCATGCAT ACAAGGAAGA GGCATATGGA ACTGTTTCAG 
GAGCTGAATC AGAAGTTTCA AACTTTGGAC AGATTTCGGG ATATACCAAA TACAAGTAGT 
ATGGAAAACC CAGCACCAAA CAAGAATCCA TGGGACACTT TCAAAAACCC CAGTGAATAC 
CAACATTATA CCACAATCAA TGTCTTAGAT ACAGAGGCAA AGGATGCTTT GGAACTGAGG 
CCAGCAGAGT GGGAGAAGTG TCTGAATTTG CCTCTGGATG TGCAAGAGGG TGACTTTCAA 
ACAGAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB3 ( XM_538991.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_538991.2 Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X1, mRNA.

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CloneID	OCe15460
Clone ID Related Accession (Same CDS sequence)	XM_538991.5 , XM_538991.6 , XM_861971.5 , XM_861971.4
Accession Version	XM_538991.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_538991.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAGAAGAAA ATCACGATAT 
TCAGACCTTG ACTTTGAGAA GGTCATGCAT ACAAGGAAGA GGCATATGGA ACTGTTTCAG 
GAGCTGAATC AGAAGTTTCA AACTTTGGAC AGATTTCGGG ATATACCAAA TACAAGTAGT 
ATGGAAAACC CAGCACCAAA CAAGAATCCA TGGGACACTT TCAAAAACCC CAGTGAATAC 
CAACATTATA CCACAATCAA TGTCTTAGAT ACAGAGGCAA AGGATGCTTT GGAACTGAGG 
CCAGCAGAGT GGGAGAAGTG TCTGAATTTG CCTCTGGATG TGCAAGAGGG TGACTTTCAA 
ACAGAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_538991.2
CDS	841..5412
Translation	MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCDSKNAFVFLQYDKNFIQIRRVFPTDFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLVLLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSPWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRMRTCQGAAVTGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSITEHLAKGQRVLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRANYTPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGIGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSSKELDESLVFVLGAVLYKNLDLILPTLRNYTVVNSKVIVVTIRPEPKTSDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHSGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGMHMPMSMNELGNQCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNTQPSMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLDQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSRSETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYQHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV

Target ORF information:

RefSeq Version	XM_538991.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_538991.6

ORF Insert Sequence:

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAGAAGAAA ATCACGATAT 
TCAGACCTTG ACTTTGAGAA GGTCATGCAT ACAAGGAAGA GGCATATGGA ACTGTTTCAG 
GAGCTGAATC AGAAGTTTCA AACTTTGGAC AGATTTCGGG ATATACCAAA TACAAGTAGT 
ATGGAAAACC CAGCACCAAA CAAGAATCCA TGGGACACTT TCAAAAACCC CAGTGAATAC 
CAACATTATA CCACAATCAA TGTCTTAGAT ACAGAGGCAA AGGATGCTTT GGAACTGAGG 
CCAGCAGAGT GGGAGAAGTG TCTGAATTTG CCTCTGGATG TGCAAGAGGG TGACTTTCAA 
ACAGAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB3 ( XM_861971.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_867064.1 Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X2, mRNA.

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CloneID	OCe15460
Clone ID Related Accession (Same CDS sequence)	XM_538991.5 , XM_538991.6 , XM_861971.5 , XM_861971.4
Accession Version	XM_861971.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_861971.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAGAAGAAA ATCACGATAT 
TCAGACCTTG ACTTTGAGAA GGTCATGCAT ACAAGGAAGA GGCATATGGA ACTGTTTCAG 
GAGCTGAATC AGAAGTTTCA AACTTTGGAC AGATTTCGGG ATATACCAAA TACAAGTAGT 
ATGGAAAACC CAGCACCAAA CAAGAATCCA TGGGACACTT TCAAAAACCC CAGTGAATAC 
CAACATTATA CCACAATCAA TGTCTTAGAT ACAGAGGCAA AGGATGCTTT GGAACTGAGG 
CCAGCAGAGT GGGAGAAGTG TCTGAATTTG CCTCTGGATG TGCAAGAGGG TGACTTTCAA 
ACAGAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_867064.1
CDS	676..5247
Translation	MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCDSKNAFVFLQYDKNFIQIRRVFPTDFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLVLLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSPWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRMRTCQGAAVTGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSITEHLAKGQRVLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRANYTPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGIGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSSKELDESLVFVLGAVLYKNLDLILPTLRNYTVVNSKVIVVTIRPEPKTSDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHSGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGMHMPMSMNELGNQCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNTQPSMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLDQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSRSETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYQHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV

Target ORF information:

RefSeq Version	XM_861971.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_861971.5

ORF Insert Sequence:

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAGAAGAAA ATCACGATAT 
TCAGACCTTG ACTTTGAGAA GGTCATGCAT ACAAGGAAGA GGCATATGGA ACTGTTTCAG 
GAGCTGAATC AGAAGTTTCA AACTTTGGAC AGATTTCGGG ATATACCAAA TACAAGTAGT 
ATGGAAAACC CAGCACCAAA CAAGAATCCA TGGGACACTT TCAAAAACCC CAGTGAATAC 
CAACATTATA CCACAATCAA TGTCTTAGAT ACAGAGGCAA AGGATGCTTT GGAACTGAGG 
CCAGCAGAGT GGGAGAAGTG TCTGAATTTG CCTCTGGATG TGCAAGAGGG TGACTTTCAA 
ACAGAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB3 ( XM_861971.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_867064.1 Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe15460
Clone ID Related Accession (Same CDS sequence)	XM_538991.5 , XM_538991.6 , XM_861971.5 , XM_861971.4
Accession Version	XM_861971.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	brain-specific angiogenesis inhibitor 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_861971.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAGAAGAAA ATCACGATAT 
TCAGACCTTG ACTTTGAGAA GGTCATGCAT ACAAGGAAGA GGCATATGGA ACTGTTTCAG 
GAGCTGAATC AGAAGTTTCA AACTTTGGAC AGATTTCGGG ATATACCAAA TACAAGTAGT 
ATGGAAAACC CAGCACCAAA CAAGAATCCA TGGGACACTT TCAAAAACCC CAGTGAATAC 
CAACATTATA CCACAATCAA TGTCTTAGAT ACAGAGGCAA AGGATGCTTT GGAACTGAGG 
CCAGCAGAGT GGGAGAAGTG TCTGAATTTG CCTCTGGATG TGCAAGAGGG TGACTTTCAA 
ACAGAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_867064.1
CDS	660..5231
Translation	MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCDSKNAFVFLQYDKNFIQIRRVFPTDFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLVLLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSPWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRMRTCQGAAVTGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSITEHLAKGQRVLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRANYTPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGIGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSSKELDESLVFVLGAVLYKNLDLILPTLRNYTVVNSKVIVVTIRPEPKTSDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHSGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGMHMPMSMNELGNQCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNTQPSMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLDQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSRSETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYQHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV

Target ORF information:

RefSeq Version	XM_861971.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_861971.4

ORF Insert Sequence:

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GTCAGATGAG TGAGCCTCAT AGCGGTTTGA CGCTCAAATG TGCCAAGTGT 
GGAGTAGTTT CAACAACAGC TTTGTCAGCC ACCACCGCCA GTAACGCCAT GGCGTCTCTG 
TGGAGTTCCT GTGTGGTGTT GCCCCTTCTG GCTCTGACGT GGATGTCTGC AGTTCTGGCC 
ATGACAGACA AACGCTCCAT ATTGTTTCAA ATACTTTTTG CTGTGTTTGA TTCATTGCAA 
GGCTTTGTGA TAGTCATGGT CCACTGCATT CTTCGGCGAG AGGTTCAGGA TGCATTTAGA 
TGCCGATTGA GAAACTGTCA GGATCCCATC AATGCTGATT CTTCAAGTTC TTTTCCTAAT 
GGGCATGCTC AAATAATGAC AGACTTTGAA AAGGATGTAG ACATTGCCTG TCGATCAGTT 
CTTCATAAGG ATATTGGTCC TTGCCGAGCA GCAACAATAA CAGGAACACT CTCTAGGATT 
TCTCTAAATG ATGATGAAGA AGAAAAGGGA ACAAATCCTG AAGGGCTTAG CTATTCCACA 
CTGCCTGGAA ACGTCATTTC CAAAGTCATT ATCCAGCAAC CCACGGGTAT GCATATGCCC 
ATGAGTATGA ATGAGCTGGG CAATCAGTGT TTGAAAAAAG AAAACAGTGA ATTGCGAAGA 
ACTGTGTACT TATGTACAGA TGACAATTTG AGAGGAGCTG ATATGGACAT AGTCCATCCT 
CAAGAAAGAA TGATGGAAAG TGACTATATC GTGATGCCCA GAAGTTCTGT AAATACCCAG 
CCATCAATGA AAGAGGAAAG CAAAATGAAT ATTGGTATGG AAACCTTGCC ACATGAAAGG 
CTATTGCACT ACAAAGTGAA TCCGGAATTC AACATGAATC CACCTGTCAT GGACCAGTTC 
AATATGAACT TAGATCAACA CCTTGCACCC CAGGAACATA TGCAGAATTT GCCCTTTGAG 
CCTCGCACAG CTGTGAAGAA TTTCATGGCC TCTGAGTTGG ATGATAATGC AGGACTATCA 
AGAAGTGAAA CTGGGTCAAC GATATCAATG AGTTCATTAG AGAGAAGAAA ATCACGATAT 
TCAGACCTTG ACTTTGAGAA GGTCATGCAT ACAAGGAAGA GGCATATGGA ACTGTTTCAG 
GAGCTGAATC AGAAGTTTCA AACTTTGGAC AGATTTCGGG ATATACCAAA TACAAGTAGT 
ATGGAAAACC CAGCACCAAA CAAGAATCCA TGGGACACTT TCAAAAACCC CAGTGAATAC 
CAACATTATA CCACAATCAA TGTCTTAGAT ACAGAGGCAA AGGATGCTTT GGAACTGAGG 
CCAGCAGAGT GGGAGAAGTG TCTGAATTTG CCTCTGGATG TGCAAGAGGG TGACTTTCAA 
ACAGAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB3 ( XM_005627521.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005627578.1 Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe15461
Clone ID Related Accession (Same CDS sequence)	XM_005627521.3 , XM_005627521.2
Accession Version	XM_005627521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GGGCGTCTCT GTGGAGTTCC TGTGTGGTGT TGCCCCTTCT GGCTCTGACG 
TGGATGTCTG CAGTTCTGGC CATGACAGAC AAACGCTCCA TATTGTTTCA AATACTTTTT 
GCTGTGTTTG ATTCATTGCA AGGCTTTGTG ATAGTCATGG TCCACTGCAT TCTTCGGCGA 
GAGGTTCAGG ATGCATTTAG ATGCCGATTG AGAAACTGTC AGGATCCCAT CAATGCTGAT 
TCTTCAAGTT CTTTTCCTAA TGGGCATGCT CAAATAATGA CAGACTTTGA AAAGGATGTA 
GACATTGCCT GTCGATCAGT TCTTCATAAG GATATTGGTC CTTGCCGAGC AGCAACAATA 
ACAGGAACAC TCTCTAGGAT TTCTCTAAAT GATGATGAAG AAGAAAAGGG AACAAATCCT 
GAAGGGCTTA GCTATTCCAC ACTGCCTGGA AACGTCATTT CCAAAGTCAT TATCCAGCAA 
CCCACGGGTA TGCATATGCC CATGAGTATG AATGAGCTGG GCAATCAGTG TTTGAAAAAA 
GAAAACAGTG AATTGCGAAG AACTGTGTAC TTATGTACAG ATGACAATTT GAGAGGAGCT 
GATATGGACA TAGTCCATCC TCAAGAAAGA ATGATGGAAA GTGACTATAT CGTGATGCCC 
AGAAGTTCTG TAAATACCCA GCCATCAATG AAAGAGGAAA GCAAAATGAA TATTGGTATG 
GAAACCTTGC CACATGAAAG GCTATTGCAC TACAAAGTGA ATCCGGAATT CAACATGAAT 
CCACCTGTCA TGGACCAGTT CAATATGAAC TTAGATCAAC ACCTTGCACC CCAGGAACAT 
ATGCAGAATT TGCCCTTTGA GCCTCGCACA GCTGTGAAGA ATTTCATGGC CTCTGAGTTG 
GATGATAATG CAGGACTATC AAGAAGTGAA ACTGGGTCAA CGATATCAAT GAGTTCATTA 
GAGAGAAGAA AATCACGATA TTCAGACCTT GACTTTGAGA AGGTCATGCA TACAAGGAAG 
AGGCATATGG AACTGTTTCA GGAGCTGAAT CAGAAGTTTC AAACTTTGGA CAGATTTCGG 
GATATACCAA ATACAAGTAG TATGGAAAAC CCAGCACCAA ACAAGAATCC ATGGGACACT 
TTCAAAAACC CCAGTGAATA CCAACATTAT ACCACAATCA ATGTCTTAGA TACAGAGGCA 
AAGGATGCTT TGGAACTGAG GCCAGCAGAG TGGGAGAAGT GTCTGAATTT GCCTCTGGAT 
GTGCAAGAGG GTGACTTTCA AACAGAAGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627578.1
CDS	841..5313
Translation	MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCDSKNAFVFLQYDKNFIQIRRVFPTDFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLVLLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSPWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRMRTCQGAAVTGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSITEHLAKGQRVLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRANYTPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGIGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSSKELDESLVFVLGAVLYKNLDLILPTLRNYTVVNSKVIVVTIRPEPKTSDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGMHMPMSMNELGNQCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNTQPSMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLDQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSRSETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYQHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV

Target ORF information:

RefSeq Version	XM_005627521.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627521.3

ORF Insert Sequence:

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GGGCGTCTCT GTGGAGTTCC TGTGTGGTGT TGCCCCTTCT GGCTCTGACG 
TGGATGTCTG CAGTTCTGGC CATGACAGAC AAACGCTCCA TATTGTTTCA AATACTTTTT 
GCTGTGTTTG ATTCATTGCA AGGCTTTGTG ATAGTCATGG TCCACTGCAT TCTTCGGCGA 
GAGGTTCAGG ATGCATTTAG ATGCCGATTG AGAAACTGTC AGGATCCCAT CAATGCTGAT 
TCTTCAAGTT CTTTTCCTAA TGGGCATGCT CAAATAATGA CAGACTTTGA AAAGGATGTA 
GACATTGCCT GTCGATCAGT TCTTCATAAG GATATTGGTC CTTGCCGAGC AGCAACAATA 
ACAGGAACAC TCTCTAGGAT TTCTCTAAAT GATGATGAAG AAGAAAAGGG AACAAATCCT 
GAAGGGCTTA GCTATTCCAC ACTGCCTGGA AACGTCATTT CCAAAGTCAT TATCCAGCAA 
CCCACGGGTA TGCATATGCC CATGAGTATG AATGAGCTGG GCAATCAGTG TTTGAAAAAA 
GAAAACAGTG AATTGCGAAG AACTGTGTAC TTATGTACAG ATGACAATTT GAGAGGAGCT 
GATATGGACA TAGTCCATCC TCAAGAAAGA ATGATGGAAA GTGACTATAT CGTGATGCCC 
AGAAGTTCTG TAAATACCCA GCCATCAATG AAAGAGGAAA GCAAAATGAA TATTGGTATG 
GAAACCTTGC CACATGAAAG GCTATTGCAC TACAAAGTGA ATCCGGAATT CAACATGAAT 
CCACCTGTCA TGGACCAGTT CAATATGAAC TTAGATCAAC ACCTTGCACC CCAGGAACAT 
ATGCAGAATT TGCCCTTTGA GCCTCGCACA GCTGTGAAGA ATTTCATGGC CTCTGAGTTG 
GATGATAATG CAGGACTATC AAGAAGTGAA ACTGGGTCAA CGATATCAAT GAGTTCATTA 
GAGAGAAGAA AATCACGATA TTCAGACCTT GACTTTGAGA AGGTCATGCA TACAAGGAAG 
AGGCATATGG AACTGTTTCA GGAGCTGAAT CAGAAGTTTC AAACTTTGGA CAGATTTCGG 
GATATACCAA ATACAAGTAG TATGGAAAAC CCAGCACCAA ACAAGAATCC ATGGGACACT 
TTCAAAAACC CCAGTGAATA CCAACATTAT ACCACAATCA ATGTCTTAGA TACAGAGGCA 
AAGGATGCTT TGGAACTGAG GCCAGCAGAG TGGGAGAAGT GTCTGAATTT GCCTCTGGAT 
GTGCAAGAGG GTGACTTTCA AACAGAAGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB3 ( XM_005627521.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005627578.1 Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X4, mRNA.

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CloneID	OCe15461
Clone ID Related Accession (Same CDS sequence)	XM_005627521.3 , XM_005627521.2
Accession Version	XM_005627521.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	brain-specific angiogenesis inhibitor 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GGGCGTCTCT GTGGAGTTCC TGTGTGGTGT TGCCCCTTCT GGCTCTGACG 
TGGATGTCTG CAGTTCTGGC CATGACAGAC AAACGCTCCA TATTGTTTCA AATACTTTTT 
GCTGTGTTTG ATTCATTGCA AGGCTTTGTG ATAGTCATGG TCCACTGCAT TCTTCGGCGA 
GAGGTTCAGG ATGCATTTAG ATGCCGATTG AGAAACTGTC AGGATCCCAT CAATGCTGAT 
TCTTCAAGTT CTTTTCCTAA TGGGCATGCT CAAATAATGA CAGACTTTGA AAAGGATGTA 
GACATTGCCT GTCGATCAGT TCTTCATAAG GATATTGGTC CTTGCCGAGC AGCAACAATA 
ACAGGAACAC TCTCTAGGAT TTCTCTAAAT GATGATGAAG AAGAAAAGGG AACAAATCCT 
GAAGGGCTTA GCTATTCCAC ACTGCCTGGA AACGTCATTT CCAAAGTCAT TATCCAGCAA 
CCCACGGGTA TGCATATGCC CATGAGTATG AATGAGCTGG GCAATCAGTG TTTGAAAAAA 
GAAAACAGTG AATTGCGAAG AACTGTGTAC TTATGTACAG ATGACAATTT GAGAGGAGCT 
GATATGGACA TAGTCCATCC TCAAGAAAGA ATGATGGAAA GTGACTATAT CGTGATGCCC 
AGAAGTTCTG TAAATACCCA GCCATCAATG AAAGAGGAAA GCAAAATGAA TATTGGTATG 
GAAACCTTGC CACATGAAAG GCTATTGCAC TACAAAGTGA ATCCGGAATT CAACATGAAT 
CCACCTGTCA TGGACCAGTT CAATATGAAC TTAGATCAAC ACCTTGCACC CCAGGAACAT 
ATGCAGAATT TGCCCTTTGA GCCTCGCACA GCTGTGAAGA ATTTCATGGC CTCTGAGTTG 
GATGATAATG CAGGACTATC AAGAAGTGAA ACTGGGTCAA CGATATCAAT GAGTTCATTA 
GAGAGAAGAA AATCACGATA TTCAGACCTT GACTTTGAGA AGGTCATGCA TACAAGGAAG 
AGGCATATGG AACTGTTTCA GGAGCTGAAT CAGAAGTTTC AAACTTTGGA CAGATTTCGG 
GATATACCAA ATACAAGTAG TATGGAAAAC CCAGCACCAA ACAAGAATCC ATGGGACACT 
TTCAAAAACC CCAGTGAATA CCAACATTAT ACCACAATCA ATGTCTTAGA TACAGAGGCA 
AAGGATGCTT TGGAACTGAG GCCAGCAGAG TGGGAGAAGT GTCTGAATTT GCCTCTGGAT 
GTGCAAGAGG GTGACTTTCA AACAGAAGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627578.1
CDS	825..5297
Translation	MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCDSKNAFVFLQYDKNFIQIRRVFPTDFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLVLLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSPWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRMRTCQGAAVTGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSITEHLAKGQRVLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRANYTPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGIGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSSKELDESLVFVLGAVLYKNLDLILPTLRNYTVVNSKVIVVTIRPEPKTSDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGMHMPMSMNELGNQCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNTQPSMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLDQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSRSETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYQHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV

Target ORF information:

RefSeq Version	XM_005627521.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B3 (ADGRB3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627521.2

ORF Insert Sequence:

ATGAAGGCTG TTCGTAACCT GCTGATTTAT ATATTTTCCA CCTATCTCCT GGTTATGTTT 
GGATTTAATG CTGCCCAAGA CTTCTGGTGT TCAACTTTGG TGAAGGGAGT CATTTATGGA 
TCGTATTCTG TAAGTGAAAT GTTTCCCAAA AACTTTACAA ACTGCACTTG GACGCTGGAA 
AATCCAGATC CAACCAAATA TAGCATTTAC CTGAAATTTT CCAAAAAGGA CCTTAGCTGC 
TCTAACTTTT CCCTCTTGGC TTATCAGTTT GATCATTTTT CCCATGAAAA AATAAAGGAT 
CTTTTAAGAA AGAATCATTC TATAATGCAA CTCTGCGATT CCAAGAATGC TTTCGTTTTT 
CTACAATATG ATAAAAATTT TATTCAAATA CGTCGGGTGT TTCCAACTGA TTTCCCAGGA 
TTACAGAAAA AAGGGGAAGA AGATCAGAAA TCTTTTTTTG AGTTTTTGGT ATTGAACAAG 
GTGAGCCCAA GCCAGTTTGG TTGCCATGTA TTATGCACTT GGTTGGAGAG CTGCTTAAAA 
TCAGAAAATG GGAGAACAGA ATCATGTGGG ATCATGTATA CAAAATGCAC CTGCCCTCAG 
CATTTGGGAG AGTGGGGGAT CGACGACCAG TCGCTGGTTT TGTTAAATAA CGTGGTGTTA 
CCCCTGAATG AGCAGACCGA GGGCTGTCTG ACCCAGGAGC TGCAAACCAC CCAGGTCTGC 
AATCTTACCA GGGAGGCCAA GCGACCGCCC AAAGAAGAAT TTGGAATGAT GGGAGATCAT 
ACAATTAAAA GTCAGCGACC TCGATCTGTT CATGAAAAAA GGGTCCCTCA GGAGCAAGCT 
GATGCTGCTA AATTTATGGC ACAAACTGGT GAATCTGGTG TGGAAGAGTG GTCCCAGTGG 
AGCACATGCT CAGTTACTTG TGGTCAAGGG TCGCAGGTGC GAACCAGAAC TTGTGTATCA 
CCTTACGGGA CACACTGCAG CGGCCCATTA AGAGAATCAA GGGTTTGCAA TAACACTGCC 
CTCTGTCCAG TACATGGAGT ATGGGAGGAG TGGTCACCAT GGAGTTTATG TTCATTTACA 
TGTGGTCGAG GCCAAAGAAC AAGAACAAGG TCGTGCACAC CTCCTCAGTA TGGAGGAAGG 
CCCTGTGAAG GACCTGAAAC ACATCATAAG CCTTGTAATA TTGCTCTGTG CCCAGTTGAT 
GGACAGTGGC AAGAGTGGAG TCCATGGAGT CAGTGCTCAG TGACATGCTC GAATGGGACC 
CAGCAGAGAA GCAGGCAGTG TACTGCAGCA GCTCATGGGG GCTCTGAATG CAGAGGGCCA 
TGGGCAGAAA GCAGAGAGTG CTATAACCCT GAGTGTACAG CAAATGGTCA GTGGAATCAG 
TGGGGTCATT GGAGCGGATG TTCCAAATCC TGTGATGGCG GCTGGGAAAG GCGAATGAGG 
ACTTGTCAAG GTGCTGCAGT AACAGGGCAG CAATGTGAAG GCACAGGTGA AGAAGTGAGA 
AGATGCAGTG AGCAACGTTG TCCTGCACCT TATGAAATAT GCCCAGAGGA TTATCTGATG 
TCGATGGTGT GGAAAAGGAC CCCGGCAGGT GACTTGGCAT TCAATCAGTG CCCGCTGAAT 
GCCACAGGCA CCACTAGCAG ACGCTGCTCT CTCAGCCTTC ATGGAGTGGC CTTCTGGGAA 
CAGCCGAGCT TTGCAAGATG CATATCAAAT GAGTACAGAC ACTTGCAGCA TTCAATCACA 
GAACACCTTG CTAAGGGGCA ACGAGTATTA GCAGGTGATG GAATGTCCCA GGTGACCAAG 
ACACTGTTGG ATTTAACTCA GAGGAAAAAT TTCTATGCAG GTGATCTTCT GATGTCTGTG 
GAAATTCTCA GAAATGTAAC AGACACATTT AAAAGGGCAA ATTACACCCC TGCGTCTGAT 
GGTGTCCAGA ACTTCTTTCA AATAGTTAGC AACCTTCTAG ATGAAGAAAA CAAGGAAAAA 
TGGGAGGATG CACAACAGAT TTATCCTGGC TCAATAGAGT TAATGCAGGT GATTGAAGAT 
TTTATACACA TTGTTGGAAT AGGGATGATG GACTTTCAGA ATTCATACTT AATGACTGGA 
AATGTAGTGG CTAGCATTCA GAAGCTTCCT GCAGCCTCTG TTCTAACAGA CATCAACTTC 
CCAATGAAAG GACGAAAGGG AATGGTTGAC TGGGCAAGAA ACTCAGAAGA CAGGGTAGTC 
ATTCCAAAAA GCATTTTTAC TCCTGTGTCA TCATCAAAAG AATTAGATGA ATCATTGGTA 
TTTGTTCTTG GAGCAGTCCT GTACAAAAAC TTAGATCTAA TTTTGCCCAC TTTGAGAAAT 
TACACTGTCG TTAATTCCAA AGTCATCGTG GTCACAATAA GGCCTGAACC CAAAACATCT 
GATTCGTTCC TGGAAATAGA ACTAGCTCAT TTGGCTAATG GTACTTTGAA TCCCTATTGT 
GTGTTATGGG ATGACTCAAA AACGAACGAG TCTTTGGGAA CGTGGTCCAC CCAGGGATGT 
AAAACTGTGC TTACCGATGC ATCCCATACG AAATGCTTAT GTGATCGTCT CTCTACCTTC 
GCCATTTTGG CTCAGCAACC TAGAGAAATA ATCATGGAAT CCTCTGGTAC ACCTTCAGTT 
ACCCTAATAG TAGGCAGTGG CCTTTCTTGC TTGGCCTTGA TCACCCTCGC AGTTGTCTAT 
GCAGCATTAT GGAGGTACAT ACGCTCTGAA AGATCTATAA TTCTAATTAA CTTCTGCCTG 
TCTATCATCT CATCCAACAT CCTCATACTG GTTGGACAGA CTCAGACACA TAATAAGAGC 
ATCTGTACAA CCACAACTGC ATTTTTGCAC TTTTTCTTCC TGGCTTCATT CTGTTGGGTT 
TTGACTGAGG CGTGGCAGTC ATATATGGCT GTAACTGGAA AAATTAGGAC ACGGCTTATA 
AGGAAACGTT TTTTGTGCCT TGGATGGGGT TTACCAGCAT TAGTAGTGGC CACATCAGTA 
GGCTTCACAA GGACGAAAGG ATATGGCACT GATCACTACT GCTGGCTCTC TCTTGAAGGA 
GGACTACTCT ACGCTTTTGT TGGACCTGCA GCCGCTGTTG TCCTGGTCAA CATGGTGATT 
GGCATTTTGG TATTTAATAA ACTTGTTTCC AGAGATGGGA TCCTAGATAA AAAGCTCAAA 
CACAGAGCCG GGGCGTCTCT GTGGAGTTCC TGTGTGGTGT TGCCCCTTCT GGCTCTGACG 
TGGATGTCTG CAGTTCTGGC CATGACAGAC AAACGCTCCA TATTGTTTCA AATACTTTTT 
GCTGTGTTTG ATTCATTGCA AGGCTTTGTG ATAGTCATGG TCCACTGCAT TCTTCGGCGA 
GAGGTTCAGG ATGCATTTAG ATGCCGATTG AGAAACTGTC AGGATCCCAT CAATGCTGAT 
TCTTCAAGTT CTTTTCCTAA TGGGCATGCT CAAATAATGA CAGACTTTGA AAAGGATGTA 
GACATTGCCT GTCGATCAGT TCTTCATAAG GATATTGGTC CTTGCCGAGC AGCAACAATA 
ACAGGAACAC TCTCTAGGAT TTCTCTAAAT GATGATGAAG AAGAAAAGGG AACAAATCCT 
GAAGGGCTTA GCTATTCCAC ACTGCCTGGA AACGTCATTT CCAAAGTCAT TATCCAGCAA 
CCCACGGGTA TGCATATGCC CATGAGTATG AATGAGCTGG GCAATCAGTG TTTGAAAAAA 
GAAAACAGTG AATTGCGAAG AACTGTGTAC TTATGTACAG ATGACAATTT GAGAGGAGCT 
GATATGGACA TAGTCCATCC TCAAGAAAGA ATGATGGAAA GTGACTATAT CGTGATGCCC 
AGAAGTTCTG TAAATACCCA GCCATCAATG AAAGAGGAAA GCAAAATGAA TATTGGTATG 
GAAACCTTGC CACATGAAAG GCTATTGCAC TACAAAGTGA ATCCGGAATT CAACATGAAT 
CCACCTGTCA TGGACCAGTT CAATATGAAC TTAGATCAAC ACCTTGCACC CCAGGAACAT 
ATGCAGAATT TGCCCTTTGA GCCTCGCACA GCTGTGAAGA ATTTCATGGC CTCTGAGTTG 
GATGATAATG CAGGACTATC AAGAAGTGAA ACTGGGTCAA CGATATCAAT GAGTTCATTA 
GAGAGAAGAA AATCACGATA TTCAGACCTT GACTTTGAGA AGGTCATGCA TACAAGGAAG 
AGGCATATGG AACTGTTTCA GGAGCTGAAT CAGAAGTTTC AAACTTTGGA CAGATTTCGG 
GATATACCAA ATACAAGTAG TATGGAAAAC CCAGCACCAA ACAAGAATCC ATGGGACACT 
TTCAAAAACC CCAGTGAATA CCAACATTAT ACCACAATCA ATGTCTTAGA TACAGAGGCA 
AAGGATGCTT TGGAACTGAG GCCAGCAGAG TGGGAGAAGT GTCTGAATTT GCCTCTGGAT 
GTGCAAGAGG GTGACTTTCA AACAGAAGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ADGRB3 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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