KDM3B cDNA ORF clone, Bos taurus(cattle)

The following KDM3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa05491 XM_003586301.5
Latest version!
Bos taurus lysine demethylase 3B (KDM3B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa05491 XM_003582422.4
Latest version!
Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa05492 XM_005209425.4
Latest version!
Bos taurus lysine demethylase 3B (KDM3B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa05492 XM_005199056.3
Latest version!
Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa60541 XM_010807343.3
Latest version!
Bos taurus lysine demethylase 3B (KDM3B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa60541 XM_010798452.2
Latest version!
Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa60542 XM_010798453.2
Latest version!
Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa60542 XM_010807344.3
Latest version!
Bos taurus lysine demethylase 3B (KDM3B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OBa60541 XM_010807343.2 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa05492 XM_005209425.3 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa60542 XM_010807344.2 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa05491 XM_003586301.4 Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa05491
    Clone ID Related Accession (Same CDS sequence) XM_003586301.4 , XM_003582422.4 , XM_003586301.5
    Accession Version XM_003586301.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104034.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586301.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586349.1
    CDS20..5299
    Translation

    Target ORF information:

    RefSeq Version XM_003586301.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586301.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05491
    Clone ID Related Accession (Same CDS sequence) XM_003586301.4 , XM_003582422.4 , XM_003586301.5
    Accession Version XM_003582422.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582422.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582470.1
    CDS20..5299
    Translation

    Target ORF information:

    RefSeq Version XM_003582422.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003582422.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05491
    Clone ID Related Accession (Same CDS sequence) XM_003586301.4 , XM_003582422.4 , XM_003586301.5
    Accession Version XM_003586301.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586301.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586349.1
    CDS57..5336
    Translation

    Target ORF information:

    RefSeq Version XM_003586301.5
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586301.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05492
    Clone ID Related Accession (Same CDS sequence) XM_005209425.3 , XM_005209425.4 , XM_005199056.3
    Accession Version XM_005209425.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104034.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209425.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209482.1
    CDS18..4697
    Translation

    Target ORF information:

    RefSeq Version XM_005209425.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209425.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05492
    Clone ID Related Accession (Same CDS sequence) XM_005209425.3 , XM_005209425.4 , XM_005199056.3
    Accession Version XM_005209425.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209425.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209482.1
    CDS16..4695
    Translation

    Target ORF information:

    RefSeq Version XM_005209425.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 3B (KDM3B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209425.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05492
    Clone ID Related Accession (Same CDS sequence) XM_005209425.3 , XM_005209425.4 , XM_005199056.3
    Accession Version XM_005199056.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199113.1
    CDS18..4697
    Translation

    Target ORF information:

    RefSeq Version XM_005199056.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199056.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGAGACAGA AGAGATGGGT GATGAAGAGG TTTTCTCCTG GTTGAAGTGT
    GCAAAGGGGC AGTCCCATGA GCCAGAGAAT CTCATGCCCA CACAGATAAT CCCTGGCACA
    GCTCTTTACA ATATTGGAGA CATGGTACAT GCTGCCCGGG GCAAGTGGGG AATTAAAGCA
    AACTGCCCTT GTATTAGTCG GCAGAACAAA TCTGTGTTGA GACCTGCTGT CACTAATGGG
    ATGTCACAGC TTCCTAGCAT AAACCCTAGT GCCTCTTCTG GAAATGAAAC CACCTTCTCA
    AGCGGAGGAG GAACTGCACC GGGAGCACAA CCAGAGACAG ACCATGTTCC CAAAGCCGAC
    AGTACTGACA GCAGATCAGA TGAGCCTCTG AAAGCAGACA GTTCGACATC AAATAGCAAT
    AGTGAGCTAA AAGCCATCAG GCCCCCTTGC CCTGACACAG CCCCACCCTC CTCTGCCCTG
    CACTGGTTGG CGGATTTAGC AACTCAGAAG GCTAAAGAAG AAACAAAAGA AGCAGGGTCC
    CTGAGGTCGG TGCTCAATAA AGAGTCTCAT TCACCCTTTG GGCTGGATTC GTTCAACTCC
    ACTGCAAAGG TCTCTCCGTT GACTCCAAAG CTTTTTAACA GTCTGTTACT GGGTCCCACT
    GCCTCCAACA ACAAAACTGA AGGCTCTAGC CTTCGAGACC TCCTCCACTC CGGGCCAGGA
    AAGCTTCCTC AAACCCCCTT GGACACAGGC ATACCCTTTC CCCCGGTCTT CTCTACATCC
    TCAGCAGGAG TGAAGAACAA GGCCAGCCTA CCCAACTTCC TTGACCACAT CATTGCCTCA
    GTGGTAGAAA ATAAGAAAAC CTCAGATGCT GCAAAGCGGG CCTGTAACTT GACTGATACC
    CAAAAGGAAG TGAAGGAGAT GGTGATGGGG TTAAATGTGC TGGACCCCCA TACCTCTCAT
    TCCTGGCTCT GTGATGGAAG ACTTTTGTGT CTTCATGACC CCAGCAACAA AAACAATTGG
    AAGATCTTCC GGGAGTGTTG GAAGCAAGGC CAGCCAGTGC TGGTTTCAGG AGTACATAAA
    AAGCTCAAGT CTGAACTCTG GAAGCCAGAA GCCTTTAGCC AGGAATTTGG AGACCAGGAT
    GTGGACTTGG TGAACTGCAG GAACTGTGCT ATAATTTCCG ATGTGAAAGT TCGGGATTTC
    TGGGATGGCT TCGAGATCAT ATGCAAACGA TTAAGGTCAG AAGATGGGCA GCCAATGGTG
    CTCAAACTCA AGGACTGGCC TCCTGGGGAA GATTTTCGGG ACATGATGCC AACAAGGTTT
    GAAGATCTGA TGGAGAACCT TCCTCTGCCA GAATATACCA AGCGAGATGG CAGGCTCAAT
    CTGGCCTCCA GGCTACCCAG CTATTTTGTA AGGCCCGATC TGGGCCCCAA GATGTACAAT
    GCCTACGGCT TGATAACAGC AGAAGACAGA AGAGTTGGCA CAACAAATCT TCACTTAGAT
    GTGTCTGATG CAGTTAATGT GATGGTGTAT GTTGGGATCC CCATCGGGGA GGGTGCTCAT
    GATGAAGAGG TTCTCAAAAC AATTGACGAG GGAGATGCTG ATGAGGTGAC AAAGCAAAGG
    ATTCATGATG GAAAGGAGAA ACCCGGTGCT TTGTGGCATA TCTATGCAGC CAAGGATGCA
    GAGAAGATCC GGGAGCTGCT CCGAAAGGTT GGAGAAGAGC AAGGCCAAGA GAATCCCCCT
    GATCATGATC CAATTCATGA CCAAAGTTGG TACTTGGACC AGACCCTACG TAAGCGGCTC
    TATGAGGAAT ATGGTGTGCA AGGCTGGGCC ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC
    ATACCTGCTG GAGCCCCACA CCAGGTGCAC AACCTATATA GTTGCATCAA AGTCGCAGAA
    GACTTCGTGT CTCCAGAACA CGTAAAGCAC TGTTTTCGCC TGACGCAGGA ATTCAGGCAT
    CTCTCTAACA CTCACACAAA CCATGAGGAT AAACTGCAGG TGAAGAACAT CATTTATCAC
    GCAGTGAAAG ATGCTGTTGG CACCCTCAAG GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60541
    Clone ID Related Accession (Same CDS sequence) XM_010807343.2 , XM_010807343.3 , XM_010798452.2
    Accession Version XM_010807343.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104034.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805645.1
    CDS20..5329
    Translation

    Target ORF information:

    RefSeq Version XM_010807343.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807343.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60541
    Clone ID Related Accession (Same CDS sequence) XM_010807343.2 , XM_010807343.3 , XM_010798452.2
    Accession Version XM_010807343.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807343.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805645.1
    CDS16..5325
    Translation

    Target ORF information:

    RefSeq Version XM_010807343.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807343.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60541
    Clone ID Related Accession (Same CDS sequence) XM_010807343.2 , XM_010807343.3 , XM_010798452.2
    Accession Version XM_010798452.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796754.1
    CDS20..5329
    Translation

    Target ORF information:

    RefSeq Version XM_010798452.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798452.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGGGGG GTACGTTAAA AGCAGTAAAA TCTTCCAAAG GAAAGAAGAA GAGAGAGAGC
    ATAGAGGGGA AAGATGGCCG GAGGAGGAAA AGTGCTTCGG ACTCTGGGTG TGACCCTGCA
    TCAAAGAAAT TGAAAGGAGA CAGGGGTGAA GTAGACAGTA ATGGGAGCGA TGGAGGCGAG
    GCAAGCCGAG GGCCCTGGAA AGGAGGGAAT GCCAGTGGAG AGCCAGGGCT GGATCAGAGA
    ACCAAGCAGC CACCGACTAC ATTTGTCCCC CAGATTAACC GCAGCATTCG CTTTGCCACT
    TACACCAAAG AAAACGGCAG GACTCTGGTG GTGCAGGATG AGCCTGTGGG TGGGGACACA
    CCTCTACCTT TCACTCCGTA TTCTGCAGCC ACAGGTCAGA CACCTTTGGC CCCAGAGGTG
    GGTGGAGCCG AAAATAAAGA GGCAGGAAAA ACACTGGAAC AAGTTGGCCA GTGTATGGTG
    GCTTCAGCAG CTGTGGTCAC TACCGCCAGC TCCACCCCAA CCACGGTGAG GATCTCAGAC
    ACTGGCCTTG CAACAGGGAC TGGGCCGGAA AAACAGAAAG GCAGCTGGTC GCAGGCCCCA
    GGAGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60542
    Clone ID Related Accession (Same CDS sequence) XM_010807344.3 , XM_010807344.2 , XM_010798453.2
    Accession Version XM_010807344.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807344.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805646.1
    CDS16..4725
    Translation

    Target ORF information:

    RefSeq Version XM_010807344.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 3B (KDM3B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807344.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60542
    Clone ID Related Accession (Same CDS sequence) XM_010807344.3 , XM_010807344.2 , XM_010798453.2
    Accession Version XM_010807344.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104034.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805646.1
    CDS19..4728
    Translation

    Target ORF information:

    RefSeq Version XM_010807344.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807344.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60542
    Clone ID Related Accession (Same CDS sequence) XM_010807344.3 , XM_010807344.2 , XM_010798453.2
    Accession Version XM_010798453.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 3B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796755.1
    CDS19..4728
    Translation

    Target ORF information:

    RefSeq Version XM_010798453.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 3B (KDM3B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGACG CGGCAGCCTC CCCGGTGGGC AAGCGGCTGC TGCTTCTGTT CGCGGACACC 
    GCGGGCTCGT CCTCGGCCTC GGTCCCCGCG GCGGCGGCGG CGGCGGCGGG CGGAGATCCG
    GGGCCTGCGC TGCGCACTCG GGCCTGGCGG GCCGGCACGG TGCGGGCCAT GAGCGGGGCG
    GTGCCCCAGG ACCTAGCGAT CTTTGTAGAA TTTGATGGCT GTAACTGGAA GCAGCACTCC
    TGGGTTAAAG TTCACGCTGA AGAAGTTATT GTACTTTTGC TGGAAGGGTC TCTAGTATGG
    GCACCTCGGA AGGACCCAGT CCTCATCCAG GGCACTCGAG TCTCAATTGC ACAGTGGCCA
    GCCCTGACTT TCACTCCCCT GGTAGATAAA CTCGGTTTGG GTTCTGTGGT TCCAGTTGAA
    TACCTTCTGG ATCGAGAGCT TCGTTTCCTG TCAGATGCTA ATGGGTTGCA TTTGTTCCAG
    ATGGGAACAG ATGGCCAAAA CCAGATTCTT TTGGAACATG CTGCTCTGAG AGACACAGTT
    AATGCTTTGA TCAGTGACCA AAAGCTACAA GAGATATTCA GCCGAGGTCC CTACAGTGTT
    CAAGGTCACA GGGTCAAAGT GTACCAGCCA GAGGGAGAAG AAAGTTGGCT CTGTGGTGTT
    GTAAGCCATC AAGATTCCAT CACCCGTCTT ATGGAAGTGT CTGTAACTGA GAGTGGTGAG
    ATCAAGTCCG TAGATCCCAG ACTGATCCAT GTGATGCTCG TGGATAATTC GGCGCCTCAG
    AGCGAGAATT CAAGGAATTC TGTCCTGGCC TCTTCTGGAT TTGGAGCATC ACTCCCGGGT
    TCATCACAAG CTTTGACTTT TGGAAGTGGA AGGAGCCAGT CCAATGGGGT TCTTGCCACA
    GAGAACAAAC CTTTGGGCTT CTCTTTTGGC TGTAGCCCAG CACCAGAGTC TCAGAAAGAC
    TCTGATCTCT CCAAAAACTT GTTTTTTCAA TGCATGTCCC AAACTTTACC CACCAGTAAC
    TACTTCACTA CCGTTTCAGA GAATTTGGCT GATGATTCCT CCAGTCGAGA CTCATTTAAA
    CAAAGCCTTG AGAGCCTCTG TAAAGGAAGA TCCACTCTTG GAGCAGACAC TAAATCAGGC
    TCTAAGGCTG GCAGCTCTGT GGATCGGAAA GTGCCTGCAG AGTCCATGCC CACCCTCACG
    CCAGCCTTCC CACGGAGCCT CCTAAATGCC CGCACCCCAG ACAGTCATGA AAATCTATTT
    TTACAGCCCC CTAAACTGTC CCGAGAAGAG CCTTCTAACC CTTTCTTGGC ATTTGTGGAG
    AAAGTTGAAC ACAGTCCTTT CAGCAGCTTT GCATCTCAGG CATCAGGTAG CTCTTCCTCT
    GCTACCACTG TCACCTCCAA GGCAGCACCC AGCTGGCCCG AGTCTCACTC CTCTACAGAT
    TCAGCATCTT TAGCAAAGAA GAAAACCCTC TTCATCACAA CTGACTCCTC CAAACTGGTG
    TCTGGTGTTC TGGGCTCAGC TCTTACCACT GGGGGGCCAA GCCTCTCTGC CATGGGGAAT
    GGCCGCTCCA GTTCACCCAC CAGCGGCCTC ACCCAGCCCA TTGAGATGCC TACTCTCTCC
    TCCAGCCCCA CAGAGGAGAG GCCAACTGTG GGGCCTGGGC AGCAGGACAA TCCTCTCCTT
    AAAACCTTTA GTAACGTCTT TGGCAGGCAC TCAGGCAGCT TTCTGTCCTC CCCAGCAGAT
    TTTTCACAGG AGAACAAAGC TCCTTTTGAA GCTGTGAAAA GGTTCTCACT GGATGAGCGA
    AGCTTGGCTT GCAGACAGGA CTCAGACTCC AGTACCAATA GTGACCTGTC AGATTTAAGC
    GACTCTGAGG AGCAGCTCCA AGCCAAGACC GGTCTAAAGG GGATTCCGGA ACACCTGATG
    GGGAAGCTGG GCCCCAATGG GGAACGCAGT GCTGAGCTGT TGCTGGGAAA AGGCAAAGGG
    AAGCAGGCCC CCAAGGGCCG GCCTCGGACT GCTCCCTTGA AAGTGGGCCA GTCAGTGCTG
    AAAGATGTGA GCAAAGTAAA GAAGCTGAAG CAGTCCGGAG AGCCCTTCCT GCAGGATGGG
    TCTTGCATCA ATGTAGCCCC TCACCTGCAC AAGTGTCGTG AGTGCCGCCT GGAGCGGTAT
    CGGAAGTTTA AGGAGCAGGA GCAAGATGAT TCCACTGTGG CCTGCCGCTT CTTTCACTTC
    CGGAGGTTGA TCTTCACTCG AAAAGGGGTA CTCCGAGTGG AAGGGTTTCT GAGTCCCCAG
    CAAAGTGACC CTGATGCCAT GAACCTGTGG ATTCCCTCTT CCTCCCTAGC AGAAGGAATA
    GACCTAGAAA CCTCAAAATA CATTCTGGCC AATGTTGGAG ACCAGTTCTG CCAACTTGTG
    ATGTCTGAGA AGGAGGCCAT GATGATGGTG GAGCCACACC AGAAAGTGGC ATGGAAGCGA
    GCGGTCCGAG GTGTACGGGA GATGTGTGAT GTATGTGAAA CAACCCTCTT CAACATCCAC
    TGGGTTTGTC GCAAATGCGG ATTTGGGGTC TGCCTTGACT GTTACAGGCT CAGGAAAAGC
    CGACCGCGCA GTGGTGAGCA AATGTTGTCC TATACGGCTG AAGAGACAGA AGAGATGGGT
    GATGAAGAGG TTTTCTCCTG GTTGAAGTGT GCAAAGGGGC AGTCCCATGA GCCAGAGAAT
    CTCATGCCCA CACAGATAAT CCCTGGCACA GCTCTTTACA ATATTGGAGA CATGGTACAT
    GCTGCCCGGG GCAAGTGGGG AATTAAAGCA AACTGCCCTT GTATTAGTCG GCAGAACAAA
    TCTGTGTTGA GACCTGCTGT CACTAATGGG ATGTCACAGC TTCCTAGCAT AAACCCTAGT
    GCCTCTTCTG GAAATGAAAC CACCTTCTCA AGCGGAGGAG GAACTGCACC GGGAGCACAA
    CCAGAGACAG ACCATGTTCC CAAAGCCGAC AGTACTGACA GCAGATCAGA TGAGCCTCTG
    AAAGCAGACA GTTCGACATC AAATAGCAAT AGTGAGCTAA AAGCCATCAG GCCCCCTTGC
    CCTGACACAG CCCCACCCTC CTCTGCCCTG CACTGGTTGG CGGATTTAGC AACTCAGAAG
    GCTAAAGAAG AAACAAAAGA AGCAGGGTCC CTGAGGTCGG TGCTCAATAA AGAGTCTCAT
    TCACCCTTTG GGCTGGATTC GTTCAACTCC ACTGCAAAGG TCTCTCCGTT GACTCCAAAG
    CTTTTTAACA GTCTGTTACT GGGTCCCACT GCCTCCAACA ACAAAACTGA AGGCTCTAGC
    CTTCGAGACC TCCTCCACTC CGGGCCAGGA AAGCTTCCTC AAACCCCCTT GGACACAGGC
    ATACCCTTTC CCCCGGTCTT CTCTACATCC TCAGCAGGAG TGAAGAACAA GGCCAGCCTA
    CCCAACTTCC TTGACCACAT CATTGCCTCA GTGGTAGAAA ATAAGAAAAC CTCAGATGCT
    GCAAAGCGGG CCTGTAACTT GACTGATACC CAAAAGGAAG TGAAGGAGAT GGTGATGGGG
    TTAAATGTGC TGGACCCCCA TACCTCTCAT TCCTGGCTCT GTGATGGAAG ACTTTTGTGT
    CTTCATGACC CCAGCAACAA AAACAATTGG AAGATCTTCC GGGAGTGTTG GAAGCAAGGC
    CAGCCAGTGC TGGTTTCAGG AGTACATAAA AAGCTCAAGT CTGAACTCTG GAAGCCAGAA
    GCCTTTAGCC AGGAATTTGG AGACCAGGAT GTGGACTTGG TGAACTGCAG GAACTGTGCT
    ATAATTTCCG ATGTGAAAGT TCGGGATTTC TGGGATGGCT TCGAGATCAT ATGCAAACGA
    TTAAGGTCAG AAGATGGGCA GCCAATGGTG CTCAAACTCA AGGACTGGCC TCCTGGGGAA
    GATTTTCGGG ACATGATGCC AACAAGGTTT GAAGATCTGA TGGAGAACCT TCCTCTGCCA
    GAATATACCA AGCGAGATGG CAGGCTCAAT CTGGCCTCCA GGCTACCCAG CTATTTTGTA
    AGGCCCGATC TGGGCCCCAA GATGTACAAT GCCTACGGCT TGATAACAGC AGAAGACAGA
    AGAGTTGGCA CAACAAATCT TCACTTAGAT GTGTCTGATG CAGTTAATGT GATGGTGTAT
    GTTGGGATCC CCATCGGGGA GGGTGCTCAT GATGAAGAGG TTCTCAAAAC AATTGACGAG
    GGAGATGCTG ATGAGGTGAC AAAGCAAAGG ATTCATGATG GAAAGGAGAA ACCCGGTGCT
    TTGTGGCATA TCTATGCAGC CAAGGATGCA GAGAAGATCC GGGAGCTGCT CCGAAAGGTT
    GGAGAAGAGC AAGGCCAAGA GAATCCCCCT GATCATGATC CAATTCATGA CCAAAGTTGG
    TACTTGGACC AGACCCTACG TAAGCGGCTC TATGAGGAAT ATGGTGTGCA AGGCTGGGCC
    ATTGTTCAGT TCCTGGGAGA TGCTGTCTTC ATACCTGCTG GAGCCCCACA CCAGGTGCAC
    AACCTATATA GTTGCATCAA AGTCGCAGAA GACTTCGTGT CTCCAGAACA CGTAAAGCAC
    TGTTTTCGCC TGACGCAGGA ATTCAGGCAT CTCTCTAACA CTCACACAAA CCATGAGGAT
    AAACTGCAGG TGAAGAACAT CATTTATCAC GCAGTGAAAG ATGCTGTTGG CACCCTCAAG
    GCTCATGAGT CCAAGCTGGC AAGATCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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