EVC2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	EVC2
Entrez Gene ID	280834
Full Name	EvC ciliary complex subunit 2
Synonyms	LBN
General protein information	Preferred Names EvC ciliary complex subunit 2 Names limbin Ellis van Creveld syndrome 2 (limbin)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024992973.1	XP_024848741.1	limbin isoform X2
XM_024992974.1	XP_024848742.1	limbin isoform X2
XM_024992972.1	XP_024848740.1	limbin isoform X1
XM_024992975.1	XP_024848743.1	limbin isoform X2
NM_173927.1	NP_776352.1	limbin

Pathways from BioSystems

Homologies

Homo sapiens (human)	EVC2	NP_667338.3
Pan troglodytes (chimpanzee)	EVC2	XP_001155684.1
Canis lupus familiaris (dog)	EVC2	XP_005618640.1
Bos taurus (cattle)	EVC2	NP_776352.1
Xenopus tropicalis (tropical clawed frog)	evc2	XP_002939100.2
Mus musculus (house mouse)	Evc2	NP_666032.1
Rattus norvegicus (Norway rat)	Evc2	NP_001099482.1
Gallus gallus (chicken)	EVC2	NP_001073217.1
	EVC2	XP_001118862.2

Gene Ontology
Bibliography

Related articles in PubMed

Deletion in the EVC2 gene causes chondrodysplastic dwarfism in Tyrolean Grey cattle.
Murgiano L, Jagannathan V, Benazzi C, Bolcato M, Brunetti B, Muscatello LV, Dittmer K, Piffer C, Gentile A, Dr?gem?ller C
PloS one9(4)e94861(2014)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Positional cloning of the gene LIMBIN responsible for bovine chondrodysplastic dwarfism.
Takeda H, Takami M, Oguni T, Tsuji T, Yoneda K, Sato H, Ihara N, Itoh T, Kata SR, Mishina Y, Womack JE, Moritomo Y, Sugimoto Y, Kunieda T
Proceedings of the National Academy of Sciences of the United States of America99(16)10549-54(2002 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The presence of a 2 bp deletion in exon 19 (c.2993_2994ACdel) led to a premature stop codon in the coding sequence of bovine EVC2, and was concordant with the recessive pattern of inheritance in affected and carrier animals.
Title: Deletion in the EVC2 gene causes chondrodysplastic dwarfism in Tyrolean Grey cattle.

The following EVC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EVC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41620	NM_173927.1 Latest version!	Bos taurus EvC ciliary complex subunit 2 (EVC2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa235097	XM_024992972.1 Latest version!	Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa235098	XM_024992973.1 Latest version!	Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa235098	XM_024992975.1 Latest version!	Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa235098	XM_024992974.1 Latest version!	Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EVC2 ( NM_173927.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776352.1 Bos taurus EvC ciliary complex subunit 2 (EVC2), mRNA.

Price & Availability↑

CloneID	OBa41620
Clone ID Related Accession (Same CDS sequence)	NM_173927.1
Accession Version	NM_173927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	limbin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB083065.1. On Mar 29, 2005 this sequence version replaced XM_589441.1. ##Evidence-Data-START## Transcript exon combination :: AB083065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGAAATTCA GTAAGGAAAT TGAAGTCTTT AACCCACCCC TGGCTTCTTC AGCCTCTAGT 
GGGCCATGGG TTCATTCTGT CTTTGCTTTT ACACACTCAT GGCCTAGGAA GACCTTGTTT 
AAAAGAGACT CTGCTGTAAC ACACCGCCTG TATGGAGACA TTTCAAGAGA CTTTCAAGGG 
ACTTCTGAGA ATGGAGTAAT TTTTCAGAAA TGTGCAGTGG TATCTGTGCA GAGTGAGTGG 
CCATCGGCCC ACGTGCGACT GTTTGTGAAC AACACCAGGA CACCCACGGC CGCCAACCTC 
TCTGACCTGT TCTTACTCGA CAACATCACA GGCCTCACGA TCAGGGAGTC GGCTGGAAAC 
CAGACCTCGA GAGGCTTCCA GGCTTTCAGG AAGAAGTTTC TGCAAGTTGG AGACTCCTTC 
TCCGTCAGCT ACACAGCCTC GCTGGAGGCC AGAGACGTCG GGAGTGGAGA CATCCTGCTG 
CTTCCTGCCC AGCTSTCCTT CCAGAGTTCG TCACCGAACA GAACCCAGCT AAAAGCCCCC 
TTCACCATAA CAGCAGAAGA AAAGATCACG GTTCTGCCAA ACCACGGCCT CCACGCAGCA 
GGGTTCTGCG TGGCCTTCAT CCTCTCCCTG GTGCTGACCT GGGCTGTGCT TTTTTTCATG 
GTTCGATACC AGTGTGTGAA GGGAAGCTCG CTTACCAGAC ATCAGGTTCA GCATCACGAA 
AACAAGCTGG AGCACTCCCA GTTCACCTCA GCTGATGGTG TGAATGAAGA TCTCGCTCTC 
AATGACCAAA TGATAGACAT TCTATCTTCC GAGGACCCCG GGAGCATGCT TCAAGCCTTA 
GAAGAGTTGG AAATTGCAAC CCTAAATCGG GCAGACTCAG ATCTGGAAGC TTGTCGAACT 
CAAATCAGCA AAGATATCAT TGCTCTTCTG CTGAAAAATC TGACCAGCAG TGGCCAGCTC 
TCCCCTCAGG TGGAGAGGAG GATGGGTGCT GTGTTCAAAA AGCAGTTTCT GTTGCTGGAG 
AAAGAAATTC AAGAGGAGTA TGATCGGAAG ATGGTGGCTC TGACAGCTGA GTGTGACCTG 
GAAACAAGAA AGAAGACAGA AAGCCAGTAC CAGAGGGAGA TGGCAGCCAT GGAGGAAGCA 
GAAGAGGTGC TGAAACGTGT TAGTGAGAGG TCCGCCGTCG AGTGCAGCAG CCTCCTGCGG 
ACCCTGCACG GCCTGGAGCA GGAACACCTG AGGAGGTCGC TCGCTCTACA GCAGGAGGAG 
GACCTTGCCA AGGCCCACAG GCAGCTGGCC ATTTTCCAGA GGAACGAACT GCACAATATC 
TTTTTTACCC AGATAAAAAG TGCTATTTTC AAAGGCGAGC TGAAGCCAGA GGCAGCAAAA 
ATGCTGCTGC AAGATTACTC TAAAATACAG GAGAGTGTCG AAGAGTTAAT GGACTTTTTC 
CAGGCTAGTA AGAGATACCA TCTCAGTAAA AGATTCGGCC ACAGAGAGTA CCTGGTCCAG 
AATATCCAGT CATCAGAGAC CCGTATGCAA GGCCTGCTGA GCACTGCGTC TGCCCAGCTG 
ACCCTCCTCA TCCAGAAACA CGAGAGAGCA GGCTACTTGG ATGAAGATCA GATGCAAGTA 
CTCTTGGAGC GGGCTCAAAC AGAAGTTTTT TCTATAAAAC AGAAGTTGGA CAATGACTTG 
AAGCAGGAAA AGAAAAAACT CCACCAAAAA CTAATAATTA AGAGAAGACG GGAGATGCTG 
CAAAAGCACA AGGAACAGCG GAGGGAGCAG CTATCCATCG CTGAAGCCTC TGGGGCTGCT 
GAGGATGCCG GCCAGTACCT GGGCCAGTGG CGGGGCCTGA TGGCTGAGCA CAGCGCCGCC 
CTGGAGGAGC TGCAGGAGCG CCTGGACCAG GCTGCCCTGG ACGAGCTCAG GGCCCTGACC 
CTCTCGCTGT CCGAGAAAGC CACGGAGGAG CTGCGGCGCC TGCAGAACTC AGGAATGACC 
CAGGAGCTAC TCAAGCGTGG GGTGCCTTGG CTCTTCCTGC AGCAGATCCT GGAGGAGCAC 
AGCAGGGACC TGGCAGCCAG GGCCGAGAGG CTGGAGGGCG AGGAGAGGGA CCGGGGCCAG 
GAGGGCGTCC AGAGCGTGAG GCAGAGACTG AAGGACGATG CGCTGGAGGC CTCCACGGAG 
GAGCAGGCGG AGCTGAGACA CTGGGAGCAC TTGATCTTCA CGAAGCTCTG CTCTTCTGCC 
TTCTCCCTGT CCGAAGAGGA GCTGCTTGGG ATGAGGCAGG AAGTTCACGG CTGCTTCGCT 
CAGATGGACA GGAGCTTGGC CCTCCCCAAA ATCCGGGCCC GAGTCCTGCT GCAACGATTC 
CAGACAGCTT GGCGAGAAGC AGAGTTCCTC AAACTGGACC AGGCCATGAC TGCCCCTGAG 
CTGCAGCCAC AGTCCAAGGC CAGAAAGCCA CGCTCCAAGA GCAGAAGCAA GATAGACCTT 
TTGAAGAAGT GCACTGAAGA TAAAATTCAG CTGTTCAAGG AACAGGCTCC CGAAGACCTG 
GTTGAAAAGG TCCGAGGAGA ATTGCTTCGG GAGAGAGTGC ARCAGCTGGA GGCCCAGGAG 
GGACTCTTTG CCGAGTCCCT TGTCTCCCTG CAGTTCCAGA AGGCAGCGAG GATGGCCAGA 
ACCCTATGGG CGTACACCGC CCTCCTGAGC ATCCAGGACC TGCTTCTGGA GGAGCTGAAC 
TCATCAGAGA CCCTGACCAA GTCAGCCTGC ATGCAGATCC TGGAGTCTCA CAGCCCGGAG 
CTCCAGGAGC TGGAGAGAAA GCTGGAGGAC CAGCTGGCAC ACCAGGAGGC GGCCCAGCTG 
CAGCGGGCAC TGGACAGTTG GCAGCAATGG GCAGGTGAAG GACCTGCGCT TCTGCAGGAA 
CCAGAGGAGA CAGATTCTGA AAGGCACGTC TCCGCTGTCC TGCAGCGAGC CCTTAGCAAG 
GGTCAGAAGC TCCTGGAGTA TCACCAGCAG AGTTTGAGAG AGGAGCAAGA AGACAGTGTC 
GTGCTAGAAG ATTTGCTGGA GAATATGGAG ACTGACACCT TTGTGACCCT GTACGGCCAG 
GAGCTGAGAC TGGCGTCGTA CCTGTCGAAG CTGACGATGC TGCCGGGGGG CACGCTGCGC 
CGGCTCCTGA CCGTGGCTCT GCCCGCAGCC TCGCAGGCCG AGCTGCTGGC GGTGCTGGAC 
TCCGTGGGCC AGAAGCATCA AGACCACTCG GTGGAGAACG ATGGCAGCAG GGTGCAGGCT 
GACCTGGGCA GGAGGGGGAA ACACCAGGGT TGGTGGCAAG CCTTAGAGAG CAAACTTCGT 
GGAGAACTGA TAAACAGAGG ATTAGAGAAG ATGCTCTGGG CTCAGAAGAG GAAGGAGAGC 
ATATTAAAGA AGACGTGTCC CCCTCTCAGA GAGAGGGTGA TATTCTCTGG AAAAAGAAGT 
TGGCCGCACC TGTCCCTGGA GTCCACTGAT GAACTGACCC CAGTGCCCAT TGTAGGGGCA 
GAAGCTGTTG ACTTATTAAA CACGGGTGAA AAGCTCTTTA TCTTCAGAAA TCCAAAGGAG 
CCTGAGATCT CACTGCACGT TCCTCCCAGG AAGAAGAAGA AGAACTTTCT GAATGCCAAA 
AAGGCCGCGT GGGCCTTGGG CTTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776352.1
CDS	1..3630
Translation	MKFSKEIEVFNPPLASSASSGPWVHSVFAFTHSWPRKTLFKRDSAVTHRLYGDISRDFQGTSENGVIFQKCAVVSVQSEWPSAHVRLFVNNTRTPTAANLSDLFLLDNITGLTIRESAGNQTSRGFQAFRKKFLQVGDSFSVSYTASLEARDVGSGDILLLPAQnullSFQSSSPNRTQLKAPFTITAEEKITVLPNHGLHAAGFCVAFILSLVLTWAVLFFMVRYQCVKGSSLTRHQVQHHENKLEHSQFTSADGVNEDLALNDQMIDILSSEDPGSMLQALEELEIATLNRADSDLEACRTQISKDIIALLLKNLTSSGQLSPQVERRMGAVFKKQFLLLEKEIQEEYDRKMVALTAECDLETRKKTESQYQREMAAMEEAEEVLKRVSERSAVECSSLLRTLHGLEQEHLRRSLALQQEEDLAKAHRQLAIFQRNELHNIFFTQIKSAIFKGELKPEAAKMLLQDYSKIQESVEELMDFFQASKRYHLSKRFGHREYLVQNIQSSETRMQGLLSTASAQLTLLIQKHERAGYLDEDQMQVLLERAQTEVFSIKQKLDNDLKQEKKKLHQKLIIKRRREMLQKHKEQRREQLSIAEASGAAEDAGQYLGQWRGLMAEHSAALEELQERLDQAALDELRALTLSLSEKATEELRRLQNSGMTQELLKRGVPWLFLQQILEEHSRDLAARAERLEGEERDRGQEGVQSVRQRLKDDALEASTEEQAELRHWEHLIFTKLCSSAFSLSEEELLGMRQEVHGCFAQMDRSLALPKIRARVLLQRFQTAWREAEFLKLDQAMTAPELQPQSKARKPRSKSRSKIDLLKKCTEDKIQLFKEQAPEDLVEKVRGELLRERVnullQLEAQEGLFAESLVSLQFQKAARMARTLWAYTALLSIQDLLLEELNSSETLTKSACMQILESHSPELQELERKLEDQLAHQEAAQLQRALDSWQQWAGEGPALLQEPEETDSERHVSAVLQRALSKGQKLLEYHQQSLREEQEDSVVLEDLLENMETDTFVTLYGQELRLASYLSKLTMLPGGTLRRLLTVALPAASQAELLAVLDSVGQKHQDHSVENDGSRVQADLGRRGKHQGWWQALESKLRGELINRGLEKMLWAQKRKESILKKTCPPLRERVIFSGKRSWPHLSLESTDELTPVPIVGAEAVDLLNTGEKLFIFRNPKEPEISLHVPPRKKKKNFLNAKKAAWALGLN

Target ORF information:

RefSeq Version	NM_173927.1
Organism	Bos taurus(cattle)
Definition	Bos taurus EvC ciliary complex subunit 2 (EVC2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173927.1

ORF Insert Sequence:

ATGAAATTCA GTAAGGAAAT TGAAGTCTTT AACCCACCCC TGGCTTCTTC AGCCTCTAGT 
GGGCCATGGG TTCATTCTGT CTTTGCTTTT ACACACTCAT GGCCTAGGAA GACCTTGTTT 
AAAAGAGACT CTGCTGTAAC ACACCGCCTG TATGGAGACA TTTCAAGAGA CTTTCAAGGG 
ACTTCTGAGA ATGGAGTAAT TTTTCAGAAA TGTGCAGTGG TATCTGTGCA GAGTGAGTGG 
CCATCGGCCC ACGTGCGACT GTTTGTGAAC AACACCAGGA CACCCACGGC CGCCAACCTC 
TCTGACCTGT TCTTACTCGA CAACATCACA GGCCTCACGA TCAGGGAGTC GGCTGGAAAC 
CAGACCTCGA GAGGCTTCCA GGCTTTCAGG AAGAAGTTTC TGCAAGTTGG AGACTCCTTC 
TCCGTCAGCT ACACAGCCTC GCTGGAGGCC AGAGACGTCG GGAGTGGAGA CATCCTGCTG 
CTTCCTGCCC AGCTSTCCTT CCAGAGTTCG TCACCGAACA GAACCCAGCT AAAAGCCCCC 
TTCACCATAA CAGCAGAAGA AAAGATCACG GTTCTGCCAA ACCACGGCCT CCACGCAGCA 
GGGTTCTGCG TGGCCTTCAT CCTCTCCCTG GTGCTGACCT GGGCTGTGCT TTTTTTCATG 
GTTCGATACC AGTGTGTGAA GGGAAGCTCG CTTACCAGAC ATCAGGTTCA GCATCACGAA 
AACAAGCTGG AGCACTCCCA GTTCACCTCA GCTGATGGTG TGAATGAAGA TCTCGCTCTC 
AATGACCAAA TGATAGACAT TCTATCTTCC GAGGACCCCG GGAGCATGCT TCAAGCCTTA 
GAAGAGTTGG AAATTGCAAC CCTAAATCGG GCAGACTCAG ATCTGGAAGC TTGTCGAACT 
CAAATCAGCA AAGATATCAT TGCTCTTCTG CTGAAAAATC TGACCAGCAG TGGCCAGCTC 
TCCCCTCAGG TGGAGAGGAG GATGGGTGCT GTGTTCAAAA AGCAGTTTCT GTTGCTGGAG 
AAAGAAATTC AAGAGGAGTA TGATCGGAAG ATGGTGGCTC TGACAGCTGA GTGTGACCTG 
GAAACAAGAA AGAAGACAGA AAGCCAGTAC CAGAGGGAGA TGGCAGCCAT GGAGGAAGCA 
GAAGAGGTGC TGAAACGTGT TAGTGAGAGG TCCGCCGTCG AGTGCAGCAG CCTCCTGCGG 
ACCCTGCACG GCCTGGAGCA GGAACACCTG AGGAGGTCGC TCGCTCTACA GCAGGAGGAG 
GACCTTGCCA AGGCCCACAG GCAGCTGGCC ATTTTCCAGA GGAACGAACT GCACAATATC 
TTTTTTACCC AGATAAAAAG TGCTATTTTC AAAGGCGAGC TGAAGCCAGA GGCAGCAAAA 
ATGCTGCTGC AAGATTACTC TAAAATACAG GAGAGTGTCG AAGAGTTAAT GGACTTTTTC 
CAGGCTAGTA AGAGATACCA TCTCAGTAAA AGATTCGGCC ACAGAGAGTA CCTGGTCCAG 
AATATCCAGT CATCAGAGAC CCGTATGCAA GGCCTGCTGA GCACTGCGTC TGCCCAGCTG 
ACCCTCCTCA TCCAGAAACA CGAGAGAGCA GGCTACTTGG ATGAAGATCA GATGCAAGTA 
CTCTTGGAGC GGGCTCAAAC AGAAGTTTTT TCTATAAAAC AGAAGTTGGA CAATGACTTG 
AAGCAGGAAA AGAAAAAACT CCACCAAAAA CTAATAATTA AGAGAAGACG GGAGATGCTG 
CAAAAGCACA AGGAACAGCG GAGGGAGCAG CTATCCATCG CTGAAGCCTC TGGGGCTGCT 
GAGGATGCCG GCCAGTACCT GGGCCAGTGG CGGGGCCTGA TGGCTGAGCA CAGCGCCGCC 
CTGGAGGAGC TGCAGGAGCG CCTGGACCAG GCTGCCCTGG ACGAGCTCAG GGCCCTGACC 
CTCTCGCTGT CCGAGAAAGC CACGGAGGAG CTGCGGCGCC TGCAGAACTC AGGAATGACC 
CAGGAGCTAC TCAAGCGTGG GGTGCCTTGG CTCTTCCTGC AGCAGATCCT GGAGGAGCAC 
AGCAGGGACC TGGCAGCCAG GGCCGAGAGG CTGGAGGGCG AGGAGAGGGA CCGGGGCCAG 
GAGGGCGTCC AGAGCGTGAG GCAGAGACTG AAGGACGATG CGCTGGAGGC CTCCACGGAG 
GAGCAGGCGG AGCTGAGACA CTGGGAGCAC TTGATCTTCA CGAAGCTCTG CTCTTCTGCC 
TTCTCCCTGT CCGAAGAGGA GCTGCTTGGG ATGAGGCAGG AAGTTCACGG CTGCTTCGCT 
CAGATGGACA GGAGCTTGGC CCTCCCCAAA ATCCGGGCCC GAGTCCTGCT GCAACGATTC 
CAGACAGCTT GGCGAGAAGC AGAGTTCCTC AAACTGGACC AGGCCATGAC TGCCCCTGAG 
CTGCAGCCAC AGTCCAAGGC CAGAAAGCCA CGCTCCAAGA GCAGAAGCAA GATAGACCTT 
TTGAAGAAGT GCACTGAAGA TAAAATTCAG CTGTTCAAGG AACAGGCTCC CGAAGACCTG 
GTTGAAAAGG TCCGAGGAGA ATTGCTTCGG GAGAGAGTGC ARCAGCTGGA GGCCCAGGAG 
GGACTCTTTG CCGAGTCCCT TGTCTCCCTG CAGTTCCAGA AGGCAGCGAG GATGGCCAGA 
ACCCTATGGG CGTACACCGC CCTCCTGAGC ATCCAGGACC TGCTTCTGGA GGAGCTGAAC 
TCATCAGAGA CCCTGACCAA GTCAGCCTGC ATGCAGATCC TGGAGTCTCA CAGCCCGGAG 
CTCCAGGAGC TGGAGAGAAA GCTGGAGGAC CAGCTGGCAC ACCAGGAGGC GGCCCAGCTG 
CAGCGGGCAC TGGACAGTTG GCAGCAATGG GCAGGTGAAG GACCTGCGCT TCTGCAGGAA 
CCAGAGGAGA CAGATTCTGA AAGGCACGTC TCCGCTGTCC TGCAGCGAGC CCTTAGCAAG 
GGTCAGAAGC TCCTGGAGTA TCACCAGCAG AGTTTGAGAG AGGAGCAAGA AGACAGTGTC 
GTGCTAGAAG ATTTGCTGGA GAATATGGAG ACTGACACCT TTGTGACCCT GTACGGCCAG 
GAGCTGAGAC TGGCGTCGTA CCTGTCGAAG CTGACGATGC TGCCGGGGGG CACGCTGCGC 
CGGCTCCTGA CCGTGGCTCT GCCCGCAGCC TCGCAGGCCG AGCTGCTGGC GGTGCTGGAC 
TCCGTGGGCC AGAAGCATCA AGACCACTCG GTGGAGAACG ATGGCAGCAG GGTGCAGGCT 
GACCTGGGCA GGAGGGGGAA ACACCAGGGT TGGTGGCAAG CCTTAGAGAG CAAACTTCGT 
GGAGAACTGA TAAACAGAGG ATTAGAGAAG ATGCTCTGGG CTCAGAAGAG GAAGGAGAGC 
ATATTAAAGA AGACGTGTCC CCCTCTCAGA GAGAGGGTGA TATTCTCTGG AAAAAGAAGT 
TGGCCGCACC TGTCCCTGGA GTCCACTGAT GAACTGACCC CAGTGCCCAT TGTAGGGGCA 
GAAGCTGTTG ACTTATTAAA CACGGGTGAA AAGCTCTTTA TCTTCAGAAA TCCAAAGGAG 
CCTGAGATCT CACTGCACGT TCCTCCCAGG AAGAAGAAGA AGAACTTTCT GAATGCCAAA 
AAGGCCGCGT GGGCCTTGGG CTTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_024992972.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848740.1 Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235097
Clone ID Related Accession (Same CDS sequence)	XM_024992972.1
Accession Version	XM_024992972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	limbin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCC CGAGGGGCCC GGGGCGGGCC ACGTGGGTGC TGGCGGGGAG CCTGCTGGCC 
GCTGCACTGG CGCTGCCCGC GGAGTCCCGA AACCTCGGCC AGCTCCCCGC GGGGCAGGTT 
CCCCTCCAGC ACCCCCTGGG CCCACAGCCT CTCCGGGACC CCCACACGGT TCCCGGGGCG 
GGGCTTGAGC GGACCGCCCA GGACTTACCC CTTGGGATTT GGCCCAGTGT GGAAAACTCT 
CACGTTACAA CTCCAGAGGA GGCACCATTC GGAATGAAAT TCAGTAAGGA AATTGAAGTC 
TTTAACCCAC CCCTGGCTTC TTCAGCCTCT AGTGGGCCAT GGGTTCATTC CGTCTTTGCT 
TTTACACACT CATGGCCTAG GAAGACCTTG TTTAAAAGAG ACTCTGCTGT AACACACCGC 
CTGTATGGAG ACATTTCAAG AGACTTTCAA GGGACTTCTG AGAATGGAGT AATTTTTCAG 
AAATGTGCAG TGGTATCTGT GCAGAGTGAG TGGCCATCGG CCCACGTGCG ACTGTTTGTG 
AACAACACCA GGACACCCAC GGCCGCCAAC CTCTCCGACC TGTTCTTACT CGACAACATC 
ACAGGCCTCA CGATCAGGGA GTCGGCTGGA AACCAGACCT CGAGAGGCTT CCAGGCTTTC 
AGGAAGAAGT TTCTGCAAGT TGGAGACTCC TTCTCCGTCA GCTACACAGC CTCGCTGGAG 
GCCAGAGACG TCGGGAGTGG AGACATCCTG CTGCTTCCTG CCCAGCTCTC CTTCCAGAGT 
TCGTCACCGA ACAGAACCCA GCTAAAAGCC CCCTTCACCA TAACAGCAGA AGAAAAGATC 
ACGGTTCTGC CAAACCACGG CCTCCACGCA GCAGGGTTCT GCGTGGCCTT CATCCTCTCC 
CTGGTGCTGA CCTGGGCTGT GCTTTTTTTC ATGGTTCGAT ACCAGTGTGT GAAGGGAAGC 
TCGCTTACCA GACATCAGGT TCAGCATCAC GAAAACAAGC TGGAGCACTC CCAGTTCACC 
TCAGCTGATG GTGTGAATGA AGATCTCGCT CTCAATGACC AAATGATAGA CATTCTATCT 
TCTGAGGACC CCGGGAGCAT GCTTCAAGCC TTAGAAGAGT TGGAAATTGC AACCCTAAAT 
CGGGCAGACT CAGATCTGGA AGCTTGTCGA ACTCAAATCA GCAAAGATAT CATTGCTCTT 
CTGCTGAAAA ATCTGACCAG CAGTGGCCAG CTCTCCCCTC AGGTGGAGAG GAGGATGGGT 
GCTGTGTTCA AAAAGCAGTT TCTGTTGCTG GAGAAAGAAA TTCAAGAGGA GTATGATCGG 
AAGATGGTGG CTCTGACAGC TGAGTGTGAC CTGGAAACAA GAAAGAAGAC AGAAAGCCAG 
TACCAGAGGG AGATGGCAGC CATGGAGGAA GCAGAAGAGG TGCTGAAACG TGTTAGTGAG 
AGGTCCGCCG TCGAGTGCAG CAGCCTCCTG CGGACCCTGC ACGGCCTGGA GCAGGAACAC 
CTGAGGAGGT CGCTCGCTCT ACAGCAGGAG GAGGACCTTG CCAAGGCCCA CAGGCAGCTG 
GCCATTTTCC AGAGGAACGA ACTGCACAAT ATCTTTTTTA CCCAGATAAA AAGTGCTATT 
TTCAAAGGCG AGCTGAAGCC AGAGGCAGCA AAAATGCTGC TGCAAGATTA CTCTAAAATA 
CAGGAGAGTG TCGAAGAGTT AATGGACTTT TTCCAGGCTA GTAAGAGATA CCATCTCAGT 
AAAAGATTCG GCCACAGAGA GTACCTGGTC CAGAATATCC AGTCATCAGA GACCCGTATG 
CAAGGCCTGC TGAGCACTGC GTCTGCCCAG CTGACCCTCC TCATCCAGAA ACACGAGAGA 
GCAGGCTACT TGGATGAAGA TCAGATGCAA GTACTCTTGG AGCGGGCTCA AACAGAAGTT 
TTTTCTATAA AACAGAAGTT GGACAATGAC TTGAAGCAGG AAAAGAAAAA ACTCCACCAA 
AAACTAATAA TTAAGAGAAG ACGGGAGATG CTGCAAAAGC ACAAGGAACA GCGGAGGGAG 
CAGCTATCCA TCGCTGAAGC CTCTGGGGCT GCTGAGGATG CCGGCCAGTA CCTGGGCCAG 
TGGCGGGGCC TGATGGCTGA GCACAGCGCC GCCCTGGAGG AGCTGCAGGA GCGCCTGGAC 
CAGGCTGCCC TGGACGAGCT CAGGGCCCTG ACCCTCTCGC TGTCCGAGAA AGCCACGGAG 
GAGCTGCGGC GCCTGCAGAA CTCAGGAATG ACCCAGGAGC TACTCAAGCG TGGGGTGCCT 
TGGCTCTTCC TGCAGCAGAT CCTGGAGGAG CACAGCAGGG ACCTGGCAGC CAGGGCCGAG 
AGGCTGGAGG GCGAGGAGAG GGACCGGGGC CAGGAGGGCG TCCAGAGCGT GAGGCAGAGA 
CTGAAGGACG ATGCGCTGGA GGCCTCCACG GAGGAGCAGG CGGAGCTGAG ACACTGGGAG 
CACTTGATCT TCACGAAGCT CTGCTCTTCT GCCTTCTCCC TGTCCGAAGA GGAGCTGCTT 
GGGATGAGGC AGGAAGTTCA CGGCTGCTTC GCTCAGATGG ACAGGAGCTT GGCCCTCCCC 
AAAATCCGGG CCCGAGTCCT GCTGCAACGA TTCCAGACAG CTTGGCGAGA AGCAGAGTTC 
CTCAAACTGG ACCAGGCCAT GACTGCCCCT GAGCTGCAGC CACAGTCCAA GGCCAGAAAG 
CCACGCTCCA AGAGCAGAAG CAAGATAGAC CTTTTGAAGA AGTGCACTGA AGATAAAATT 
CAGCTGTTCA AGGAACAGGC TCCCGAAGAC CTGGTTGAAA AGGTCCGAGG AGAATTGCTT 
CGGGAGAGAG TGCAACAGCT GGAGGCCCAG GAGGGACTCT TTGCCGAGTC CCTTGTCTCC 
CTGCAGTTCC AGAAGGCAGC GAGGATGGCC AGAACCCTAT GGGCGTACAC CGCCCTCCTG 
AGCATCCAGG ACCTGCTTCT GGAGGAGCTG AACTCATCAG AGACCCTGAC CAAGTCAGCC 
TGCATGCAGA TCCTGGAGTC TCACAGCCCG GAGCTCCAGG AGCTGGAGAG AAAGCTGGAG 
GACCAGCTGG CACACCAGGA GGCGGCCCAG CTGCAGCGGG CACTGGACAG TTGGCAGCAA 
TGGGCAGGTG AAGGACCTGC GCTTCTGCAG GAACCAGAGG AGACAGATTC TGAAAGGCAC 
GTCTCCGCTG TCCTGCAGCG AGCCCTTAGC AAGGGTCAGA AGCTCCTGGA GTATCACCAG 
CAGAGTTTGA GAGAGGAGCA AGAAGACAGT GTCGTGCTAG AAGATTTGCT GGAGAATATG 
GAGACTGACA CCTTTGTGAC CCTGTACGGC CAGGAGCTGA GACTGGCGTC GTACCTGTCG 
AAGCTGACGA TGCTGCCGGG GGGCACGCTG CGCCGGCTCC TGACCGTGGC TCTGCCCGCA 
GCCTCGCAGG CCGAGCTGCT GGCGGTGCTG GACTCCGTGG GCCAGAAGCA TCAAGACCAC 
TCGGTGGAGA ACGATGGCAG CAGGGTGCAG GCTGACCTGG GCAGGAGGGG GAAACACCAG 
GGTTGGTGGC AAGCCTTAGA GAGCAAACTT CGTGGAGAAC TGATAAACAG AGGATTAGAG 
AAGATGCTCT GGGCTCAGAA GAGGAAGGAG AGCATATTAA AGAAGACGTG TCCCCCTCTC 
AGAGAGAGGG TGATATTCTC TGGAAAAAGA AGTTGGCCGC ACCTGTCCCT GGAGTCCACT 
GATGAACTGA CCCCAGTGCC CATTGTAGGG GCAGAAGCTG TTGACTTATT AAACACGGGT 
GAAAAGCTCT TTATCTTCAG AAATCCAAAG GAGCCTGAGA TCTCACTGCA CGTTCCTCCC 
AGGAAGAAGA AGAAGAACTT TCTGAATGCC AAAAAGGCCG CGTGGGCCTT GGGCTTGAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848740.1
CDS	94..3996
Translation	MDTPRGPGRATWVLAGSLLAAALALPAESRNLGQLPAGQVPLQHPLGPQPLRDPHTVPGAGLERTAQDLPLGIWPSVENSHVTTPEEAPFGMKFSKEIEVFNPPLASSASSGPWVHSVFAFTHSWPRKTLFKRDSAVTHRLYGDISRDFQGTSENGVIFQKCAVVSVQSEWPSAHVRLFVNNTRTPTAANLSDLFLLDNITGLTIRESAGNQTSRGFQAFRKKFLQVGDSFSVSYTASLEARDVGSGDILLLPAQLSFQSSSPNRTQLKAPFTITAEEKITVLPNHGLHAAGFCVAFILSLVLTWAVLFFMVRYQCVKGSSLTRHQVQHHENKLEHSQFTSADGVNEDLALNDQMIDILSSEDPGSMLQALEELEIATLNRADSDLEACRTQISKDIIALLLKNLTSSGQLSPQVERRMGAVFKKQFLLLEKEIQEEYDRKMVALTAECDLETRKKTESQYQREMAAMEEAEEVLKRVSERSAVECSSLLRTLHGLEQEHLRRSLALQQEEDLAKAHRQLAIFQRNELHNIFFTQIKSAIFKGELKPEAAKMLLQDYSKIQESVEELMDFFQASKRYHLSKRFGHREYLVQNIQSSETRMQGLLSTASAQLTLLIQKHERAGYLDEDQMQVLLERAQTEVFSIKQKLDNDLKQEKKKLHQKLIIKRRREMLQKHKEQRREQLSIAEASGAAEDAGQYLGQWRGLMAEHSAALEELQERLDQAALDELRALTLSLSEKATEELRRLQNSGMTQELLKRGVPWLFLQQILEEHSRDLAARAERLEGEERDRGQEGVQSVRQRLKDDALEASTEEQAELRHWEHLIFTKLCSSAFSLSEEELLGMRQEVHGCFAQMDRSLALPKIRARVLLQRFQTAWREAEFLKLDQAMTAPELQPQSKARKPRSKSRSKIDLLKKCTEDKIQLFKEQAPEDLVEKVRGELLRERVQQLEAQEGLFAESLVSLQFQKAARMARTLWAYTALLSIQDLLLEELNSSETLTKSACMQILESHSPELQELERKLEDQLAHQEAAQLQRALDSWQQWAGEGPALLQEPEETDSERHVSAVLQRALSKGQKLLEYHQQSLREEQEDSVVLEDLLENMETDTFVTLYGQELRLASYLSKLTMLPGGTLRRLLTVALPAASQAELLAVLDSVGQKHQDHSVENDGSRVQADLGRRGKHQGWWQALESKLRGELINRGLEKMLWAQKRKESILKKTCPPLRERVIFSGKRSWPHLSLESTDELTPVPIVGAEAVDLLNTGEKLFIFRNPKEPEISLHVPPRKKKKNFLNAKKAAWALGLN

Target ORF information:

RefSeq Version	XM_024992972.1
Organism	Bos taurus(cattle)
Definition	Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992972.1

ORF Insert Sequence:

ATGGATACCC CGAGGGGCCC GGGGCGGGCC ACGTGGGTGC TGGCGGGGAG CCTGCTGGCC 
GCTGCACTGG CGCTGCCCGC GGAGTCCCGA AACCTCGGCC AGCTCCCCGC GGGGCAGGTT 
CCCCTCCAGC ACCCCCTGGG CCCACAGCCT CTCCGGGACC CCCACACGGT TCCCGGGGCG 
GGGCTTGAGC GGACCGCCCA GGACTTACCC CTTGGGATTT GGCCCAGTGT GGAAAACTCT 
CACGTTACAA CTCCAGAGGA GGCACCATTC GGAATGAAAT TCAGTAAGGA AATTGAAGTC 
TTTAACCCAC CCCTGGCTTC TTCAGCCTCT AGTGGGCCAT GGGTTCATTC CGTCTTTGCT 
TTTACACACT CATGGCCTAG GAAGACCTTG TTTAAAAGAG ACTCTGCTGT AACACACCGC 
CTGTATGGAG ACATTTCAAG AGACTTTCAA GGGACTTCTG AGAATGGAGT AATTTTTCAG 
AAATGTGCAG TGGTATCTGT GCAGAGTGAG TGGCCATCGG CCCACGTGCG ACTGTTTGTG 
AACAACACCA GGACACCCAC GGCCGCCAAC CTCTCCGACC TGTTCTTACT CGACAACATC 
ACAGGCCTCA CGATCAGGGA GTCGGCTGGA AACCAGACCT CGAGAGGCTT CCAGGCTTTC 
AGGAAGAAGT TTCTGCAAGT TGGAGACTCC TTCTCCGTCA GCTACACAGC CTCGCTGGAG 
GCCAGAGACG TCGGGAGTGG AGACATCCTG CTGCTTCCTG CCCAGCTCTC CTTCCAGAGT 
TCGTCACCGA ACAGAACCCA GCTAAAAGCC CCCTTCACCA TAACAGCAGA AGAAAAGATC 
ACGGTTCTGC CAAACCACGG CCTCCACGCA GCAGGGTTCT GCGTGGCCTT CATCCTCTCC 
CTGGTGCTGA CCTGGGCTGT GCTTTTTTTC ATGGTTCGAT ACCAGTGTGT GAAGGGAAGC 
TCGCTTACCA GACATCAGGT TCAGCATCAC GAAAACAAGC TGGAGCACTC CCAGTTCACC 
TCAGCTGATG GTGTGAATGA AGATCTCGCT CTCAATGACC AAATGATAGA CATTCTATCT 
TCTGAGGACC CCGGGAGCAT GCTTCAAGCC TTAGAAGAGT TGGAAATTGC AACCCTAAAT 
CGGGCAGACT CAGATCTGGA AGCTTGTCGA ACTCAAATCA GCAAAGATAT CATTGCTCTT 
CTGCTGAAAA ATCTGACCAG CAGTGGCCAG CTCTCCCCTC AGGTGGAGAG GAGGATGGGT 
GCTGTGTTCA AAAAGCAGTT TCTGTTGCTG GAGAAAGAAA TTCAAGAGGA GTATGATCGG 
AAGATGGTGG CTCTGACAGC TGAGTGTGAC CTGGAAACAA GAAAGAAGAC AGAAAGCCAG 
TACCAGAGGG AGATGGCAGC CATGGAGGAA GCAGAAGAGG TGCTGAAACG TGTTAGTGAG 
AGGTCCGCCG TCGAGTGCAG CAGCCTCCTG CGGACCCTGC ACGGCCTGGA GCAGGAACAC 
CTGAGGAGGT CGCTCGCTCT ACAGCAGGAG GAGGACCTTG CCAAGGCCCA CAGGCAGCTG 
GCCATTTTCC AGAGGAACGA ACTGCACAAT ATCTTTTTTA CCCAGATAAA AAGTGCTATT 
TTCAAAGGCG AGCTGAAGCC AGAGGCAGCA AAAATGCTGC TGCAAGATTA CTCTAAAATA 
CAGGAGAGTG TCGAAGAGTT AATGGACTTT TTCCAGGCTA GTAAGAGATA CCATCTCAGT 
AAAAGATTCG GCCACAGAGA GTACCTGGTC CAGAATATCC AGTCATCAGA GACCCGTATG 
CAAGGCCTGC TGAGCACTGC GTCTGCCCAG CTGACCCTCC TCATCCAGAA ACACGAGAGA 
GCAGGCTACT TGGATGAAGA TCAGATGCAA GTACTCTTGG AGCGGGCTCA AACAGAAGTT 
TTTTCTATAA AACAGAAGTT GGACAATGAC TTGAAGCAGG AAAAGAAAAA ACTCCACCAA 
AAACTAATAA TTAAGAGAAG ACGGGAGATG CTGCAAAAGC ACAAGGAACA GCGGAGGGAG 
CAGCTATCCA TCGCTGAAGC CTCTGGGGCT GCTGAGGATG CCGGCCAGTA CCTGGGCCAG 
TGGCGGGGCC TGATGGCTGA GCACAGCGCC GCCCTGGAGG AGCTGCAGGA GCGCCTGGAC 
CAGGCTGCCC TGGACGAGCT CAGGGCCCTG ACCCTCTCGC TGTCCGAGAA AGCCACGGAG 
GAGCTGCGGC GCCTGCAGAA CTCAGGAATG ACCCAGGAGC TACTCAAGCG TGGGGTGCCT 
TGGCTCTTCC TGCAGCAGAT CCTGGAGGAG CACAGCAGGG ACCTGGCAGC CAGGGCCGAG 
AGGCTGGAGG GCGAGGAGAG GGACCGGGGC CAGGAGGGCG TCCAGAGCGT GAGGCAGAGA 
CTGAAGGACG ATGCGCTGGA GGCCTCCACG GAGGAGCAGG CGGAGCTGAG ACACTGGGAG 
CACTTGATCT TCACGAAGCT CTGCTCTTCT GCCTTCTCCC TGTCCGAAGA GGAGCTGCTT 
GGGATGAGGC AGGAAGTTCA CGGCTGCTTC GCTCAGATGG ACAGGAGCTT GGCCCTCCCC 
AAAATCCGGG CCCGAGTCCT GCTGCAACGA TTCCAGACAG CTTGGCGAGA AGCAGAGTTC 
CTCAAACTGG ACCAGGCCAT GACTGCCCCT GAGCTGCAGC CACAGTCCAA GGCCAGAAAG 
CCACGCTCCA AGAGCAGAAG CAAGATAGAC CTTTTGAAGA AGTGCACTGA AGATAAAATT 
CAGCTGTTCA AGGAACAGGC TCCCGAAGAC CTGGTTGAAA AGGTCCGAGG AGAATTGCTT 
CGGGAGAGAG TGCAACAGCT GGAGGCCCAG GAGGGACTCT TTGCCGAGTC CCTTGTCTCC 
CTGCAGTTCC AGAAGGCAGC GAGGATGGCC AGAACCCTAT GGGCGTACAC CGCCCTCCTG 
AGCATCCAGG ACCTGCTTCT GGAGGAGCTG AACTCATCAG AGACCCTGAC CAAGTCAGCC 
TGCATGCAGA TCCTGGAGTC TCACAGCCCG GAGCTCCAGG AGCTGGAGAG AAAGCTGGAG 
GACCAGCTGG CACACCAGGA GGCGGCCCAG CTGCAGCGGG CACTGGACAG TTGGCAGCAA 
TGGGCAGGTG AAGGACCTGC GCTTCTGCAG GAACCAGAGG AGACAGATTC TGAAAGGCAC 
GTCTCCGCTG TCCTGCAGCG AGCCCTTAGC AAGGGTCAGA AGCTCCTGGA GTATCACCAG 
CAGAGTTTGA GAGAGGAGCA AGAAGACAGT GTCGTGCTAG AAGATTTGCT GGAGAATATG 
GAGACTGACA CCTTTGTGAC CCTGTACGGC CAGGAGCTGA GACTGGCGTC GTACCTGTCG 
AAGCTGACGA TGCTGCCGGG GGGCACGCTG CGCCGGCTCC TGACCGTGGC TCTGCCCGCA 
GCCTCGCAGG CCGAGCTGCT GGCGGTGCTG GACTCCGTGG GCCAGAAGCA TCAAGACCAC 
TCGGTGGAGA ACGATGGCAG CAGGGTGCAG GCTGACCTGG GCAGGAGGGG GAAACACCAG 
GGTTGGTGGC AAGCCTTAGA GAGCAAACTT CGTGGAGAAC TGATAAACAG AGGATTAGAG 
AAGATGCTCT GGGCTCAGAA GAGGAAGGAG AGCATATTAA AGAAGACGTG TCCCCCTCTC 
AGAGAGAGGG TGATATTCTC TGGAAAAAGA AGTTGGCCGC ACCTGTCCCT GGAGTCCACT 
GATGAACTGA CCCCAGTGCC CATTGTAGGG GCAGAAGCTG TTGACTTATT AAACACGGGT 
GAAAAGCTCT TTATCTTCAG AAATCCAAAG GAGCCTGAGA TCTCACTGCA CGTTCCTCCC 
AGGAAGAAGA AGAAGAACTT TCTGAATGCC AAAAAGGCCG CGTGGGCCTT GGGCTTGAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_024992973.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848741.1 Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X2, mRNA.

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CloneID	OBa235098
Clone ID Related Accession (Same CDS sequence)	XM_024992973.1 , XM_024992974.1 , XM_024992975.1
Accession Version	XM_024992973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	limbin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATTCA GTAAGGAAAT TGAAGTCTTT AACCCACCCC TGGCTTCTTC AGCCTCTAGT 
GGGCCATGGG TTCATTCCGT CTTTGCTTTT ACACACTCAT GGCCTAGGAA GACCTTGTTT 
AAAAGAGACT CTGCTGTAAC ACACCGCCTG TATGGAGACA TTTCAAGAGA CTTTCAAGGG 
ACTTCTGAGA ATGGAGTAAT TTTTCAGAAA TGTGCAGTGG TATCTGTGCA GAGTGAGTGG 
CCATCGGCCC ACGTGCGACT GTTTGTGAAC AACACCAGGA CACCCACGGC CGCCAACCTC 
TCCGACCTGT TCTTACTCGA CAACATCACA GGCCTCACGA TCAGGGAGTC GGCTGGAAAC 
CAGACCTCGA GAGGCTTCCA GGCTTTCAGG AAGAAGTTTC TGCAAGTTGG AGACTCCTTC 
TCCGTCAGCT ACACAGCCTC GCTGGAGGCC AGAGACGTCG GGAGTGGAGA CATCCTGCTG 
CTTCCTGCCC AGCTCTCCTT CCAGAGTTCG TCACCGAACA GAACCCAGCT AAAAGCCCCC 
TTCACCATAA CAGCAGAAGA AAAGATCACG GTTCTGCCAA ACCACGGCCT CCACGCAGCA 
GGGTTCTGCG TGGCCTTCAT CCTCTCCCTG GTGCTGACCT GGGCTGTGCT TTTTTTCATG 
GTTCGATACC AGTGTGTGAA GGGAAGCTCG CTTACCAGAC ATCAGGTTCA GCATCACGAA 
AACAAGCTGG AGCACTCCCA GTTCACCTCA GCTGATGGTG TGAATGAAGA TCTCGCTCTC 
AATGACCAAA TGATAGACAT TCTATCTTCT GAGGACCCCG GGAGCATGCT TCAAGCCTTA 
GAAGAGTTGG AAATTGCAAC CCTAAATCGG GCAGACTCAG ATCTGGAAGC TTGTCGAACT 
CAAATCAGCA AAGATATCAT TGCTCTTCTG CTGAAAAATC TGACCAGCAG TGGCCAGCTC 
TCCCCTCAGG TGGAGAGGAG GATGGGTGCT GTGTTCAAAA AGCAGTTTCT GTTGCTGGAG 
AAAGAAATTC AAGAGGAGTA TGATCGGAAG ATGGTGGCTC TGACAGCTGA GTGTGACCTG 
GAAACAAGAA AGAAGACAGA AAGCCAGTAC CAGAGGGAGA TGGCAGCCAT GGAGGAAGCA 
GAAGAGGTGC TGAAACGTGT TAGTGAGAGG TCCGCCGTCG AGTGCAGCAG CCTCCTGCGG 
ACCCTGCACG GCCTGGAGCA GGAACACCTG AGGAGGTCGC TCGCTCTACA GCAGGAGGAG 
GACCTTGCCA AGGCCCACAG GCAGCTGGCC ATTTTCCAGA GGAACGAACT GCACAATATC 
TTTTTTACCC AGATAAAAAG TGCTATTTTC AAAGGCGAGC TGAAGCCAGA GGCAGCAAAA 
ATGCTGCTGC AAGATTACTC TAAAATACAG GAGAGTGTCG AAGAGTTAAT GGACTTTTTC 
CAGGCTAGTA AGAGATACCA TCTCAGTAAA AGATTCGGCC ACAGAGAGTA CCTGGTCCAG 
AATATCCAGT CATCAGAGAC CCGTATGCAA GGCCTGCTGA GCACTGCGTC TGCCCAGCTG 
ACCCTCCTCA TCCAGAAACA CGAGAGAGCA GGCTACTTGG ATGAAGATCA GATGCAAGTA 
CTCTTGGAGC GGGCTCAAAC AGAAGTTTTT TCTATAAAAC AGAAGTTGGA CAATGACTTG 
AAGCAGGAAA AGAAAAAACT CCACCAAAAA CTAATAATTA AGAGAAGACG GGAGATGCTG 
CAAAAGCACA AGGAACAGCG GAGGGAGCAG CTATCCATCG CTGAAGCCTC TGGGGCTGCT 
GAGGATGCCG GCCAGTACCT GGGCCAGTGG CGGGGCCTGA TGGCTGAGCA CAGCGCCGCC 
CTGGAGGAGC TGCAGGAGCG CCTGGACCAG GCTGCCCTGG ACGAGCTCAG GGCCCTGACC 
CTCTCGCTGT CCGAGAAAGC CACGGAGGAG CTGCGGCGCC TGCAGAACTC AGGAATGACC 
CAGGAGCTAC TCAAGCGTGG GGTGCCTTGG CTCTTCCTGC AGCAGATCCT GGAGGAGCAC 
AGCAGGGACC TGGCAGCCAG GGCCGAGAGG CTGGAGGGCG AGGAGAGGGA CCGGGGCCAG 
GAGGGCGTCC AGAGCGTGAG GCAGAGACTG AAGGACGATG CGCTGGAGGC CTCCACGGAG 
GAGCAGGCGG AGCTGAGACA CTGGGAGCAC TTGATCTTCA CGAAGCTCTG CTCTTCTGCC 
TTCTCCCTGT CCGAAGAGGA GCTGCTTGGG ATGAGGCAGG AAGTTCACGG CTGCTTCGCT 
CAGATGGACA GGAGCTTGGC CCTCCCCAAA ATCCGGGCCC GAGTCCTGCT GCAACGATTC 
CAGACAGCTT GGCGAGAAGC AGAGTTCCTC AAACTGGACC AGGCCATGAC TGCCCCTGAG 
CTGCAGCCAC AGTCCAAGGC CAGAAAGCCA CGCTCCAAGA GCAGAAGCAA GATAGACCTT 
TTGAAGAAGT GCACTGAAGA TAAAATTCAG CTGTTCAAGG AACAGGCTCC CGAAGACCTG 
GTTGAAAAGG TCCGAGGAGA ATTGCTTCGG GAGAGAGTGC AACAGCTGGA GGCCCAGGAG 
GGACTCTTTG CCGAGTCCCT TGTCTCCCTG CAGTTCCAGA AGGCAGCGAG GATGGCCAGA 
ACCCTATGGG CGTACACCGC CCTCCTGAGC ATCCAGGACC TGCTTCTGGA GGAGCTGAAC 
TCATCAGAGA CCCTGACCAA GTCAGCCTGC ATGCAGATCC TGGAGTCTCA CAGCCCGGAG 
CTCCAGGAGC TGGAGAGAAA GCTGGAGGAC CAGCTGGCAC ACCAGGAGGC GGCCCAGCTG 
CAGCGGGCAC TGGACAGTTG GCAGCAATGG GCAGGTGAAG GACCTGCGCT TCTGCAGGAA 
CCAGAGGAGA CAGATTCTGA AAGGCACGTC TCCGCTGTCC TGCAGCGAGC CCTTAGCAAG 
GGTCAGAAGC TCCTGGAGTA TCACCAGCAG AGTTTGAGAG AGGAGCAAGA AGACAGTGTC 
GTGCTAGAAG ATTTGCTGGA GAATATGGAG ACTGACACCT TTGTGACCCT GTACGGCCAG 
GAGCTGAGAC TGGCGTCGTA CCTGTCGAAG CTGACGATGC TGCCGGGGGG CACGCTGCGC 
CGGCTCCTGA CCGTGGCTCT GCCCGCAGCC TCGCAGGCCG AGCTGCTGGC GGTGCTGGAC 
TCCGTGGGCC AGAAGCATCA AGACCACTCG GTGGAGAACG ATGGCAGCAG GGTGCAGGCT 
GACCTGGGCA GGAGGGGGAA ACACCAGGGT TGGTGGCAAG CCTTAGAGAG CAAACTTCGT 
GGAGAACTGA TAAACAGAGG ATTAGAGAAG ATGCTCTGGG CTCAGAAGAG GAAGGAGAGC 
ATATTAAAGA AGACGTGTCC CCCTCTCAGA GAGAGGGTGA TATTCTCTGG AAAAAGAAGT 
TGGCCGCACC TGTCCCTGGA GTCCACTGAT GAACTGACCC CAGTGCCCAT TGTAGGGGCA 
GAAGCTGTTG ACTTATTAAA CACGGGTGAA AAGCTCTTTA TCTTCAGAAA TCCAAAGGAG 
CCTGAGATCT CACTGCACGT TCCTCCCAGG AAGAAGAAGA AGAACTTTCT GAATGCCAAA 
AAGGCCGCGT GGGCCTTGGG CTTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848741.1
CDS	201..3830
Translation	MKFSKEIEVFNPPLASSASSGPWVHSVFAFTHSWPRKTLFKRDSAVTHRLYGDISRDFQGTSENGVIFQKCAVVSVQSEWPSAHVRLFVNNTRTPTAANLSDLFLLDNITGLTIRESAGNQTSRGFQAFRKKFLQVGDSFSVSYTASLEARDVGSGDILLLPAQLSFQSSSPNRTQLKAPFTITAEEKITVLPNHGLHAAGFCVAFILSLVLTWAVLFFMVRYQCVKGSSLTRHQVQHHENKLEHSQFTSADGVNEDLALNDQMIDILSSEDPGSMLQALEELEIATLNRADSDLEACRTQISKDIIALLLKNLTSSGQLSPQVERRMGAVFKKQFLLLEKEIQEEYDRKMVALTAECDLETRKKTESQYQREMAAMEEAEEVLKRVSERSAVECSSLLRTLHGLEQEHLRRSLALQQEEDLAKAHRQLAIFQRNELHNIFFTQIKSAIFKGELKPEAAKMLLQDYSKIQESVEELMDFFQASKRYHLSKRFGHREYLVQNIQSSETRMQGLLSTASAQLTLLIQKHERAGYLDEDQMQVLLERAQTEVFSIKQKLDNDLKQEKKKLHQKLIIKRRREMLQKHKEQRREQLSIAEASGAAEDAGQYLGQWRGLMAEHSAALEELQERLDQAALDELRALTLSLSEKATEELRRLQNSGMTQELLKRGVPWLFLQQILEEHSRDLAARAERLEGEERDRGQEGVQSVRQRLKDDALEASTEEQAELRHWEHLIFTKLCSSAFSLSEEELLGMRQEVHGCFAQMDRSLALPKIRARVLLQRFQTAWREAEFLKLDQAMTAPELQPQSKARKPRSKSRSKIDLLKKCTEDKIQLFKEQAPEDLVEKVRGELLRERVQQLEAQEGLFAESLVSLQFQKAARMARTLWAYTALLSIQDLLLEELNSSETLTKSACMQILESHSPELQELERKLEDQLAHQEAAQLQRALDSWQQWAGEGPALLQEPEETDSERHVSAVLQRALSKGQKLLEYHQQSLREEQEDSVVLEDLLENMETDTFVTLYGQELRLASYLSKLTMLPGGTLRRLLTVALPAASQAELLAVLDSVGQKHQDHSVENDGSRVQADLGRRGKHQGWWQALESKLRGELINRGLEKMLWAQKRKESILKKTCPPLRERVIFSGKRSWPHLSLESTDELTPVPIVGAEAVDLLNTGEKLFIFRNPKEPEISLHVPPRKKKKNFLNAKKAAWALGLN

Target ORF information:

RefSeq Version	XM_024992973.1
Organism	Bos taurus(cattle)
Definition	Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992973.1

ORF Insert Sequence:

ATGAAATTCA GTAAGGAAAT TGAAGTCTTT AACCCACCCC TGGCTTCTTC AGCCTCTAGT 
GGGCCATGGG TTCATTCCGT CTTTGCTTTT ACACACTCAT GGCCTAGGAA GACCTTGTTT 
AAAAGAGACT CTGCTGTAAC ACACCGCCTG TATGGAGACA TTTCAAGAGA CTTTCAAGGG 
ACTTCTGAGA ATGGAGTAAT TTTTCAGAAA TGTGCAGTGG TATCTGTGCA GAGTGAGTGG 
CCATCGGCCC ACGTGCGACT GTTTGTGAAC AACACCAGGA CACCCACGGC CGCCAACCTC 
TCCGACCTGT TCTTACTCGA CAACATCACA GGCCTCACGA TCAGGGAGTC GGCTGGAAAC 
CAGACCTCGA GAGGCTTCCA GGCTTTCAGG AAGAAGTTTC TGCAAGTTGG AGACTCCTTC 
TCCGTCAGCT ACACAGCCTC GCTGGAGGCC AGAGACGTCG GGAGTGGAGA CATCCTGCTG 
CTTCCTGCCC AGCTCTCCTT CCAGAGTTCG TCACCGAACA GAACCCAGCT AAAAGCCCCC 
TTCACCATAA CAGCAGAAGA AAAGATCACG GTTCTGCCAA ACCACGGCCT CCACGCAGCA 
GGGTTCTGCG TGGCCTTCAT CCTCTCCCTG GTGCTGACCT GGGCTGTGCT TTTTTTCATG 
GTTCGATACC AGTGTGTGAA GGGAAGCTCG CTTACCAGAC ATCAGGTTCA GCATCACGAA 
AACAAGCTGG AGCACTCCCA GTTCACCTCA GCTGATGGTG TGAATGAAGA TCTCGCTCTC 
AATGACCAAA TGATAGACAT TCTATCTTCT GAGGACCCCG GGAGCATGCT TCAAGCCTTA 
GAAGAGTTGG AAATTGCAAC CCTAAATCGG GCAGACTCAG ATCTGGAAGC TTGTCGAACT 
CAAATCAGCA AAGATATCAT TGCTCTTCTG CTGAAAAATC TGACCAGCAG TGGCCAGCTC 
TCCCCTCAGG TGGAGAGGAG GATGGGTGCT GTGTTCAAAA AGCAGTTTCT GTTGCTGGAG 
AAAGAAATTC AAGAGGAGTA TGATCGGAAG ATGGTGGCTC TGACAGCTGA GTGTGACCTG 
GAAACAAGAA AGAAGACAGA AAGCCAGTAC CAGAGGGAGA TGGCAGCCAT GGAGGAAGCA 
GAAGAGGTGC TGAAACGTGT TAGTGAGAGG TCCGCCGTCG AGTGCAGCAG CCTCCTGCGG 
ACCCTGCACG GCCTGGAGCA GGAACACCTG AGGAGGTCGC TCGCTCTACA GCAGGAGGAG 
GACCTTGCCA AGGCCCACAG GCAGCTGGCC ATTTTCCAGA GGAACGAACT GCACAATATC 
TTTTTTACCC AGATAAAAAG TGCTATTTTC AAAGGCGAGC TGAAGCCAGA GGCAGCAAAA 
ATGCTGCTGC AAGATTACTC TAAAATACAG GAGAGTGTCG AAGAGTTAAT GGACTTTTTC 
CAGGCTAGTA AGAGATACCA TCTCAGTAAA AGATTCGGCC ACAGAGAGTA CCTGGTCCAG 
AATATCCAGT CATCAGAGAC CCGTATGCAA GGCCTGCTGA GCACTGCGTC TGCCCAGCTG 
ACCCTCCTCA TCCAGAAACA CGAGAGAGCA GGCTACTTGG ATGAAGATCA GATGCAAGTA 
CTCTTGGAGC GGGCTCAAAC AGAAGTTTTT TCTATAAAAC AGAAGTTGGA CAATGACTTG 
AAGCAGGAAA AGAAAAAACT CCACCAAAAA CTAATAATTA AGAGAAGACG GGAGATGCTG 
CAAAAGCACA AGGAACAGCG GAGGGAGCAG CTATCCATCG CTGAAGCCTC TGGGGCTGCT 
GAGGATGCCG GCCAGTACCT GGGCCAGTGG CGGGGCCTGA TGGCTGAGCA CAGCGCCGCC 
CTGGAGGAGC TGCAGGAGCG CCTGGACCAG GCTGCCCTGG ACGAGCTCAG GGCCCTGACC 
CTCTCGCTGT CCGAGAAAGC CACGGAGGAG CTGCGGCGCC TGCAGAACTC AGGAATGACC 
CAGGAGCTAC TCAAGCGTGG GGTGCCTTGG CTCTTCCTGC AGCAGATCCT GGAGGAGCAC 
AGCAGGGACC TGGCAGCCAG GGCCGAGAGG CTGGAGGGCG AGGAGAGGGA CCGGGGCCAG 
GAGGGCGTCC AGAGCGTGAG GCAGAGACTG AAGGACGATG CGCTGGAGGC CTCCACGGAG 
GAGCAGGCGG AGCTGAGACA CTGGGAGCAC TTGATCTTCA CGAAGCTCTG CTCTTCTGCC 
TTCTCCCTGT CCGAAGAGGA GCTGCTTGGG ATGAGGCAGG AAGTTCACGG CTGCTTCGCT 
CAGATGGACA GGAGCTTGGC CCTCCCCAAA ATCCGGGCCC GAGTCCTGCT GCAACGATTC 
CAGACAGCTT GGCGAGAAGC AGAGTTCCTC AAACTGGACC AGGCCATGAC TGCCCCTGAG 
CTGCAGCCAC AGTCCAAGGC CAGAAAGCCA CGCTCCAAGA GCAGAAGCAA GATAGACCTT 
TTGAAGAAGT GCACTGAAGA TAAAATTCAG CTGTTCAAGG AACAGGCTCC CGAAGACCTG 
GTTGAAAAGG TCCGAGGAGA ATTGCTTCGG GAGAGAGTGC AACAGCTGGA GGCCCAGGAG 
GGACTCTTTG CCGAGTCCCT TGTCTCCCTG CAGTTCCAGA AGGCAGCGAG GATGGCCAGA 
ACCCTATGGG CGTACACCGC CCTCCTGAGC ATCCAGGACC TGCTTCTGGA GGAGCTGAAC 
TCATCAGAGA CCCTGACCAA GTCAGCCTGC ATGCAGATCC TGGAGTCTCA CAGCCCGGAG 
CTCCAGGAGC TGGAGAGAAA GCTGGAGGAC CAGCTGGCAC ACCAGGAGGC GGCCCAGCTG 
CAGCGGGCAC TGGACAGTTG GCAGCAATGG GCAGGTGAAG GACCTGCGCT TCTGCAGGAA 
CCAGAGGAGA CAGATTCTGA AAGGCACGTC TCCGCTGTCC TGCAGCGAGC CCTTAGCAAG 
GGTCAGAAGC TCCTGGAGTA TCACCAGCAG AGTTTGAGAG AGGAGCAAGA AGACAGTGTC 
GTGCTAGAAG ATTTGCTGGA GAATATGGAG ACTGACACCT TTGTGACCCT GTACGGCCAG 
GAGCTGAGAC TGGCGTCGTA CCTGTCGAAG CTGACGATGC TGCCGGGGGG CACGCTGCGC 
CGGCTCCTGA CCGTGGCTCT GCCCGCAGCC TCGCAGGCCG AGCTGCTGGC GGTGCTGGAC 
TCCGTGGGCC AGAAGCATCA AGACCACTCG GTGGAGAACG ATGGCAGCAG GGTGCAGGCT 
GACCTGGGCA GGAGGGGGAA ACACCAGGGT TGGTGGCAAG CCTTAGAGAG CAAACTTCGT 
GGAGAACTGA TAAACAGAGG ATTAGAGAAG ATGCTCTGGG CTCAGAAGAG GAAGGAGAGC 
ATATTAAAGA AGACGTGTCC CCCTCTCAGA GAGAGGGTGA TATTCTCTGG AAAAAGAAGT 
TGGCCGCACC TGTCCCTGGA GTCCACTGAT GAACTGACCC CAGTGCCCAT TGTAGGGGCA 
GAAGCTGTTG ACTTATTAAA CACGGGTGAA AAGCTCTTTA TCTTCAGAAA TCCAAAGGAG 
CCTGAGATCT CACTGCACGT TCCTCCCAGG AAGAAGAAGA AGAACTTTCT GAATGCCAAA 
AAGGCCGCGT GGGCCTTGGG CTTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_024992974.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848742.1 Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa235098
Clone ID Related Accession (Same CDS sequence)	XM_024992973.1 , XM_024992974.1 , XM_024992975.1
Accession Version	XM_024992974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	limbin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATTCA GTAAGGAAAT TGAAGTCTTT AACCCACCCC TGGCTTCTTC AGCCTCTAGT 
GGGCCATGGG TTCATTCCGT CTTTGCTTTT ACACACTCAT GGCCTAGGAA GACCTTGTTT 
AAAAGAGACT CTGCTGTAAC ACACCGCCTG TATGGAGACA TTTCAAGAGA CTTTCAAGGG 
ACTTCTGAGA ATGGAGTAAT TTTTCAGAAA TGTGCAGTGG TATCTGTGCA GAGTGAGTGG 
CCATCGGCCC ACGTGCGACT GTTTGTGAAC AACACCAGGA CACCCACGGC CGCCAACCTC 
TCCGACCTGT TCTTACTCGA CAACATCACA GGCCTCACGA TCAGGGAGTC GGCTGGAAAC 
CAGACCTCGA GAGGCTTCCA GGCTTTCAGG AAGAAGTTTC TGCAAGTTGG AGACTCCTTC 
TCCGTCAGCT ACACAGCCTC GCTGGAGGCC AGAGACGTCG GGAGTGGAGA CATCCTGCTG 
CTTCCTGCCC AGCTCTCCTT CCAGAGTTCG TCACCGAACA GAACCCAGCT AAAAGCCCCC 
TTCACCATAA CAGCAGAAGA AAAGATCACG GTTCTGCCAA ACCACGGCCT CCACGCAGCA 
GGGTTCTGCG TGGCCTTCAT CCTCTCCCTG GTGCTGACCT GGGCTGTGCT TTTTTTCATG 
GTTCGATACC AGTGTGTGAA GGGAAGCTCG CTTACCAGAC ATCAGGTTCA GCATCACGAA 
AACAAGCTGG AGCACTCCCA GTTCACCTCA GCTGATGGTG TGAATGAAGA TCTCGCTCTC 
AATGACCAAA TGATAGACAT TCTATCTTCT GAGGACCCCG GGAGCATGCT TCAAGCCTTA 
GAAGAGTTGG AAATTGCAAC CCTAAATCGG GCAGACTCAG ATCTGGAAGC TTGTCGAACT 
CAAATCAGCA AAGATATCAT TGCTCTTCTG CTGAAAAATC TGACCAGCAG TGGCCAGCTC 
TCCCCTCAGG TGGAGAGGAG GATGGGTGCT GTGTTCAAAA AGCAGTTTCT GTTGCTGGAG 
AAAGAAATTC AAGAGGAGTA TGATCGGAAG ATGGTGGCTC TGACAGCTGA GTGTGACCTG 
GAAACAAGAA AGAAGACAGA AAGCCAGTAC CAGAGGGAGA TGGCAGCCAT GGAGGAAGCA 
GAAGAGGTGC TGAAACGTGT TAGTGAGAGG TCCGCCGTCG AGTGCAGCAG CCTCCTGCGG 
ACCCTGCACG GCCTGGAGCA GGAACACCTG AGGAGGTCGC TCGCTCTACA GCAGGAGGAG 
GACCTTGCCA AGGCCCACAG GCAGCTGGCC ATTTTCCAGA GGAACGAACT GCACAATATC 
TTTTTTACCC AGATAAAAAG TGCTATTTTC AAAGGCGAGC TGAAGCCAGA GGCAGCAAAA 
ATGCTGCTGC AAGATTACTC TAAAATACAG GAGAGTGTCG AAGAGTTAAT GGACTTTTTC 
CAGGCTAGTA AGAGATACCA TCTCAGTAAA AGATTCGGCC ACAGAGAGTA CCTGGTCCAG 
AATATCCAGT CATCAGAGAC CCGTATGCAA GGCCTGCTGA GCACTGCGTC TGCCCAGCTG 
ACCCTCCTCA TCCAGAAACA CGAGAGAGCA GGCTACTTGG ATGAAGATCA GATGCAAGTA 
CTCTTGGAGC GGGCTCAAAC AGAAGTTTTT TCTATAAAAC AGAAGTTGGA CAATGACTTG 
AAGCAGGAAA AGAAAAAACT CCACCAAAAA CTAATAATTA AGAGAAGACG GGAGATGCTG 
CAAAAGCACA AGGAACAGCG GAGGGAGCAG CTATCCATCG CTGAAGCCTC TGGGGCTGCT 
GAGGATGCCG GCCAGTACCT GGGCCAGTGG CGGGGCCTGA TGGCTGAGCA CAGCGCCGCC 
CTGGAGGAGC TGCAGGAGCG CCTGGACCAG GCTGCCCTGG ACGAGCTCAG GGCCCTGACC 
CTCTCGCTGT CCGAGAAAGC CACGGAGGAG CTGCGGCGCC TGCAGAACTC AGGAATGACC 
CAGGAGCTAC TCAAGCGTGG GGTGCCTTGG CTCTTCCTGC AGCAGATCCT GGAGGAGCAC 
AGCAGGGACC TGGCAGCCAG GGCCGAGAGG CTGGAGGGCG AGGAGAGGGA CCGGGGCCAG 
GAGGGCGTCC AGAGCGTGAG GCAGAGACTG AAGGACGATG CGCTGGAGGC CTCCACGGAG 
GAGCAGGCGG AGCTGAGACA CTGGGAGCAC TTGATCTTCA CGAAGCTCTG CTCTTCTGCC 
TTCTCCCTGT CCGAAGAGGA GCTGCTTGGG ATGAGGCAGG AAGTTCACGG CTGCTTCGCT 
CAGATGGACA GGAGCTTGGC CCTCCCCAAA ATCCGGGCCC GAGTCCTGCT GCAACGATTC 
CAGACAGCTT GGCGAGAAGC AGAGTTCCTC AAACTGGACC AGGCCATGAC TGCCCCTGAG 
CTGCAGCCAC AGTCCAAGGC CAGAAAGCCA CGCTCCAAGA GCAGAAGCAA GATAGACCTT 
TTGAAGAAGT GCACTGAAGA TAAAATTCAG CTGTTCAAGG AACAGGCTCC CGAAGACCTG 
GTTGAAAAGG TCCGAGGAGA ATTGCTTCGG GAGAGAGTGC AACAGCTGGA GGCCCAGGAG 
GGACTCTTTG CCGAGTCCCT TGTCTCCCTG CAGTTCCAGA AGGCAGCGAG GATGGCCAGA 
ACCCTATGGG CGTACACCGC CCTCCTGAGC ATCCAGGACC TGCTTCTGGA GGAGCTGAAC 
TCATCAGAGA CCCTGACCAA GTCAGCCTGC ATGCAGATCC TGGAGTCTCA CAGCCCGGAG 
CTCCAGGAGC TGGAGAGAAA GCTGGAGGAC CAGCTGGCAC ACCAGGAGGC GGCCCAGCTG 
CAGCGGGCAC TGGACAGTTG GCAGCAATGG GCAGGTGAAG GACCTGCGCT TCTGCAGGAA 
CCAGAGGAGA CAGATTCTGA AAGGCACGTC TCCGCTGTCC TGCAGCGAGC CCTTAGCAAG 
GGTCAGAAGC TCCTGGAGTA TCACCAGCAG AGTTTGAGAG AGGAGCAAGA AGACAGTGTC 
GTGCTAGAAG ATTTGCTGGA GAATATGGAG ACTGACACCT TTGTGACCCT GTACGGCCAG 
GAGCTGAGAC TGGCGTCGTA CCTGTCGAAG CTGACGATGC TGCCGGGGGG CACGCTGCGC 
CGGCTCCTGA CCGTGGCTCT GCCCGCAGCC TCGCAGGCCG AGCTGCTGGC GGTGCTGGAC 
TCCGTGGGCC AGAAGCATCA AGACCACTCG GTGGAGAACG ATGGCAGCAG GGTGCAGGCT 
GACCTGGGCA GGAGGGGGAA ACACCAGGGT TGGTGGCAAG CCTTAGAGAG CAAACTTCGT 
GGAGAACTGA TAAACAGAGG ATTAGAGAAG ATGCTCTGGG CTCAGAAGAG GAAGGAGAGC 
ATATTAAAGA AGACGTGTCC CCCTCTCAGA GAGAGGGTGA TATTCTCTGG AAAAAGAAGT 
TGGCCGCACC TGTCCCTGGA GTCCACTGAT GAACTGACCC CAGTGCCCAT TGTAGGGGCA 
GAAGCTGTTG ACTTATTAAA CACGGGTGAA AAGCTCTTTA TCTTCAGAAA TCCAAAGGAG 
CCTGAGATCT CACTGCACGT TCCTCCCAGG AAGAAGAAGA AGAACTTTCT GAATGCCAAA 
AAGGCCGCGT GGGCCTTGGG CTTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848742.1
CDS	93..3722
Translation	MKFSKEIEVFNPPLASSASSGPWVHSVFAFTHSWPRKTLFKRDSAVTHRLYGDISRDFQGTSENGVIFQKCAVVSVQSEWPSAHVRLFVNNTRTPTAANLSDLFLLDNITGLTIRESAGNQTSRGFQAFRKKFLQVGDSFSVSYTASLEARDVGSGDILLLPAQLSFQSSSPNRTQLKAPFTITAEEKITVLPNHGLHAAGFCVAFILSLVLTWAVLFFMVRYQCVKGSSLTRHQVQHHENKLEHSQFTSADGVNEDLALNDQMIDILSSEDPGSMLQALEELEIATLNRADSDLEACRTQISKDIIALLLKNLTSSGQLSPQVERRMGAVFKKQFLLLEKEIQEEYDRKMVALTAECDLETRKKTESQYQREMAAMEEAEEVLKRVSERSAVECSSLLRTLHGLEQEHLRRSLALQQEEDLAKAHRQLAIFQRNELHNIFFTQIKSAIFKGELKPEAAKMLLQDYSKIQESVEELMDFFQASKRYHLSKRFGHREYLVQNIQSSETRMQGLLSTASAQLTLLIQKHERAGYLDEDQMQVLLERAQTEVFSIKQKLDNDLKQEKKKLHQKLIIKRRREMLQKHKEQRREQLSIAEASGAAEDAGQYLGQWRGLMAEHSAALEELQERLDQAALDELRALTLSLSEKATEELRRLQNSGMTQELLKRGVPWLFLQQILEEHSRDLAARAERLEGEERDRGQEGVQSVRQRLKDDALEASTEEQAELRHWEHLIFTKLCSSAFSLSEEELLGMRQEVHGCFAQMDRSLALPKIRARVLLQRFQTAWREAEFLKLDQAMTAPELQPQSKARKPRSKSRSKIDLLKKCTEDKIQLFKEQAPEDLVEKVRGELLRERVQQLEAQEGLFAESLVSLQFQKAARMARTLWAYTALLSIQDLLLEELNSSETLTKSACMQILESHSPELQELERKLEDQLAHQEAAQLQRALDSWQQWAGEGPALLQEPEETDSERHVSAVLQRALSKGQKLLEYHQQSLREEQEDSVVLEDLLENMETDTFVTLYGQELRLASYLSKLTMLPGGTLRRLLTVALPAASQAELLAVLDSVGQKHQDHSVENDGSRVQADLGRRGKHQGWWQALESKLRGELINRGLEKMLWAQKRKESILKKTCPPLRERVIFSGKRSWPHLSLESTDELTPVPIVGAEAVDLLNTGEKLFIFRNPKEPEISLHVPPRKKKKNFLNAKKAAWALGLN

Target ORF information:

RefSeq Version	XM_024992974.1
Organism	Bos taurus(cattle)
Definition	Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992974.1

ORF Insert Sequence:

ATGAAATTCA GTAAGGAAAT TGAAGTCTTT AACCCACCCC TGGCTTCTTC AGCCTCTAGT 
GGGCCATGGG TTCATTCCGT CTTTGCTTTT ACACACTCAT GGCCTAGGAA GACCTTGTTT 
AAAAGAGACT CTGCTGTAAC ACACCGCCTG TATGGAGACA TTTCAAGAGA CTTTCAAGGG 
ACTTCTGAGA ATGGAGTAAT TTTTCAGAAA TGTGCAGTGG TATCTGTGCA GAGTGAGTGG 
CCATCGGCCC ACGTGCGACT GTTTGTGAAC AACACCAGGA CACCCACGGC CGCCAACCTC 
TCCGACCTGT TCTTACTCGA CAACATCACA GGCCTCACGA TCAGGGAGTC GGCTGGAAAC 
CAGACCTCGA GAGGCTTCCA GGCTTTCAGG AAGAAGTTTC TGCAAGTTGG AGACTCCTTC 
TCCGTCAGCT ACACAGCCTC GCTGGAGGCC AGAGACGTCG GGAGTGGAGA CATCCTGCTG 
CTTCCTGCCC AGCTCTCCTT CCAGAGTTCG TCACCGAACA GAACCCAGCT AAAAGCCCCC 
TTCACCATAA CAGCAGAAGA AAAGATCACG GTTCTGCCAA ACCACGGCCT CCACGCAGCA 
GGGTTCTGCG TGGCCTTCAT CCTCTCCCTG GTGCTGACCT GGGCTGTGCT TTTTTTCATG 
GTTCGATACC AGTGTGTGAA GGGAAGCTCG CTTACCAGAC ATCAGGTTCA GCATCACGAA 
AACAAGCTGG AGCACTCCCA GTTCACCTCA GCTGATGGTG TGAATGAAGA TCTCGCTCTC 
AATGACCAAA TGATAGACAT TCTATCTTCT GAGGACCCCG GGAGCATGCT TCAAGCCTTA 
GAAGAGTTGG AAATTGCAAC CCTAAATCGG GCAGACTCAG ATCTGGAAGC TTGTCGAACT 
CAAATCAGCA AAGATATCAT TGCTCTTCTG CTGAAAAATC TGACCAGCAG TGGCCAGCTC 
TCCCCTCAGG TGGAGAGGAG GATGGGTGCT GTGTTCAAAA AGCAGTTTCT GTTGCTGGAG 
AAAGAAATTC AAGAGGAGTA TGATCGGAAG ATGGTGGCTC TGACAGCTGA GTGTGACCTG 
GAAACAAGAA AGAAGACAGA AAGCCAGTAC CAGAGGGAGA TGGCAGCCAT GGAGGAAGCA 
GAAGAGGTGC TGAAACGTGT TAGTGAGAGG TCCGCCGTCG AGTGCAGCAG CCTCCTGCGG 
ACCCTGCACG GCCTGGAGCA GGAACACCTG AGGAGGTCGC TCGCTCTACA GCAGGAGGAG 
GACCTTGCCA AGGCCCACAG GCAGCTGGCC ATTTTCCAGA GGAACGAACT GCACAATATC 
TTTTTTACCC AGATAAAAAG TGCTATTTTC AAAGGCGAGC TGAAGCCAGA GGCAGCAAAA 
ATGCTGCTGC AAGATTACTC TAAAATACAG GAGAGTGTCG AAGAGTTAAT GGACTTTTTC 
CAGGCTAGTA AGAGATACCA TCTCAGTAAA AGATTCGGCC ACAGAGAGTA CCTGGTCCAG 
AATATCCAGT CATCAGAGAC CCGTATGCAA GGCCTGCTGA GCACTGCGTC TGCCCAGCTG 
ACCCTCCTCA TCCAGAAACA CGAGAGAGCA GGCTACTTGG ATGAAGATCA GATGCAAGTA 
CTCTTGGAGC GGGCTCAAAC AGAAGTTTTT TCTATAAAAC AGAAGTTGGA CAATGACTTG 
AAGCAGGAAA AGAAAAAACT CCACCAAAAA CTAATAATTA AGAGAAGACG GGAGATGCTG 
CAAAAGCACA AGGAACAGCG GAGGGAGCAG CTATCCATCG CTGAAGCCTC TGGGGCTGCT 
GAGGATGCCG GCCAGTACCT GGGCCAGTGG CGGGGCCTGA TGGCTGAGCA CAGCGCCGCC 
CTGGAGGAGC TGCAGGAGCG CCTGGACCAG GCTGCCCTGG ACGAGCTCAG GGCCCTGACC 
CTCTCGCTGT CCGAGAAAGC CACGGAGGAG CTGCGGCGCC TGCAGAACTC AGGAATGACC 
CAGGAGCTAC TCAAGCGTGG GGTGCCTTGG CTCTTCCTGC AGCAGATCCT GGAGGAGCAC 
AGCAGGGACC TGGCAGCCAG GGCCGAGAGG CTGGAGGGCG AGGAGAGGGA CCGGGGCCAG 
GAGGGCGTCC AGAGCGTGAG GCAGAGACTG AAGGACGATG CGCTGGAGGC CTCCACGGAG 
GAGCAGGCGG AGCTGAGACA CTGGGAGCAC TTGATCTTCA CGAAGCTCTG CTCTTCTGCC 
TTCTCCCTGT CCGAAGAGGA GCTGCTTGGG ATGAGGCAGG AAGTTCACGG CTGCTTCGCT 
CAGATGGACA GGAGCTTGGC CCTCCCCAAA ATCCGGGCCC GAGTCCTGCT GCAACGATTC 
CAGACAGCTT GGCGAGAAGC AGAGTTCCTC AAACTGGACC AGGCCATGAC TGCCCCTGAG 
CTGCAGCCAC AGTCCAAGGC CAGAAAGCCA CGCTCCAAGA GCAGAAGCAA GATAGACCTT 
TTGAAGAAGT GCACTGAAGA TAAAATTCAG CTGTTCAAGG AACAGGCTCC CGAAGACCTG 
GTTGAAAAGG TCCGAGGAGA ATTGCTTCGG GAGAGAGTGC AACAGCTGGA GGCCCAGGAG 
GGACTCTTTG CCGAGTCCCT TGTCTCCCTG CAGTTCCAGA AGGCAGCGAG GATGGCCAGA 
ACCCTATGGG CGTACACCGC CCTCCTGAGC ATCCAGGACC TGCTTCTGGA GGAGCTGAAC 
TCATCAGAGA CCCTGACCAA GTCAGCCTGC ATGCAGATCC TGGAGTCTCA CAGCCCGGAG 
CTCCAGGAGC TGGAGAGAAA GCTGGAGGAC CAGCTGGCAC ACCAGGAGGC GGCCCAGCTG 
CAGCGGGCAC TGGACAGTTG GCAGCAATGG GCAGGTGAAG GACCTGCGCT TCTGCAGGAA 
CCAGAGGAGA CAGATTCTGA AAGGCACGTC TCCGCTGTCC TGCAGCGAGC CCTTAGCAAG 
GGTCAGAAGC TCCTGGAGTA TCACCAGCAG AGTTTGAGAG AGGAGCAAGA AGACAGTGTC 
GTGCTAGAAG ATTTGCTGGA GAATATGGAG ACTGACACCT TTGTGACCCT GTACGGCCAG 
GAGCTGAGAC TGGCGTCGTA CCTGTCGAAG CTGACGATGC TGCCGGGGGG CACGCTGCGC 
CGGCTCCTGA CCGTGGCTCT GCCCGCAGCC TCGCAGGCCG AGCTGCTGGC GGTGCTGGAC 
TCCGTGGGCC AGAAGCATCA AGACCACTCG GTGGAGAACG ATGGCAGCAG GGTGCAGGCT 
GACCTGGGCA GGAGGGGGAA ACACCAGGGT TGGTGGCAAG CCTTAGAGAG CAAACTTCGT 
GGAGAACTGA TAAACAGAGG ATTAGAGAAG ATGCTCTGGG CTCAGAAGAG GAAGGAGAGC 
ATATTAAAGA AGACGTGTCC CCCTCTCAGA GAGAGGGTGA TATTCTCTGG AAAAAGAAGT 
TGGCCGCACC TGTCCCTGGA GTCCACTGAT GAACTGACCC CAGTGCCCAT TGTAGGGGCA 
GAAGCTGTTG ACTTATTAAA CACGGGTGAA AAGCTCTTTA TCTTCAGAAA TCCAAAGGAG 
CCTGAGATCT CACTGCACGT TCCTCCCAGG AAGAAGAAGA AGAACTTTCT GAATGCCAAA 
AAGGCCGCGT GGGCCTTGGG CTTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_024992975.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848743.1 Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa235098
Clone ID Related Accession (Same CDS sequence)	XM_024992973.1 , XM_024992974.1 , XM_024992975.1
Accession Version	XM_024992975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	limbin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATTCA GTAAGGAAAT TGAAGTCTTT AACCCACCCC TGGCTTCTTC AGCCTCTAGT 
GGGCCATGGG TTCATTCCGT CTTTGCTTTT ACACACTCAT GGCCTAGGAA GACCTTGTTT 
AAAAGAGACT CTGCTGTAAC ACACCGCCTG TATGGAGACA TTTCAAGAGA CTTTCAAGGG 
ACTTCTGAGA ATGGAGTAAT TTTTCAGAAA TGTGCAGTGG TATCTGTGCA GAGTGAGTGG 
CCATCGGCCC ACGTGCGACT GTTTGTGAAC AACACCAGGA CACCCACGGC CGCCAACCTC 
TCCGACCTGT TCTTACTCGA CAACATCACA GGCCTCACGA TCAGGGAGTC GGCTGGAAAC 
CAGACCTCGA GAGGCTTCCA GGCTTTCAGG AAGAAGTTTC TGCAAGTTGG AGACTCCTTC 
TCCGTCAGCT ACACAGCCTC GCTGGAGGCC AGAGACGTCG GGAGTGGAGA CATCCTGCTG 
CTTCCTGCCC AGCTCTCCTT CCAGAGTTCG TCACCGAACA GAACCCAGCT AAAAGCCCCC 
TTCACCATAA CAGCAGAAGA AAAGATCACG GTTCTGCCAA ACCACGGCCT CCACGCAGCA 
GGGTTCTGCG TGGCCTTCAT CCTCTCCCTG GTGCTGACCT GGGCTGTGCT TTTTTTCATG 
GTTCGATACC AGTGTGTGAA GGGAAGCTCG CTTACCAGAC ATCAGGTTCA GCATCACGAA 
AACAAGCTGG AGCACTCCCA GTTCACCTCA GCTGATGGTG TGAATGAAGA TCTCGCTCTC 
AATGACCAAA TGATAGACAT TCTATCTTCT GAGGACCCCG GGAGCATGCT TCAAGCCTTA 
GAAGAGTTGG AAATTGCAAC CCTAAATCGG GCAGACTCAG ATCTGGAAGC TTGTCGAACT 
CAAATCAGCA AAGATATCAT TGCTCTTCTG CTGAAAAATC TGACCAGCAG TGGCCAGCTC 
TCCCCTCAGG TGGAGAGGAG GATGGGTGCT GTGTTCAAAA AGCAGTTTCT GTTGCTGGAG 
AAAGAAATTC AAGAGGAGTA TGATCGGAAG ATGGTGGCTC TGACAGCTGA GTGTGACCTG 
GAAACAAGAA AGAAGACAGA AAGCCAGTAC CAGAGGGAGA TGGCAGCCAT GGAGGAAGCA 
GAAGAGGTGC TGAAACGTGT TAGTGAGAGG TCCGCCGTCG AGTGCAGCAG CCTCCTGCGG 
ACCCTGCACG GCCTGGAGCA GGAACACCTG AGGAGGTCGC TCGCTCTACA GCAGGAGGAG 
GACCTTGCCA AGGCCCACAG GCAGCTGGCC ATTTTCCAGA GGAACGAACT GCACAATATC 
TTTTTTACCC AGATAAAAAG TGCTATTTTC AAAGGCGAGC TGAAGCCAGA GGCAGCAAAA 
ATGCTGCTGC AAGATTACTC TAAAATACAG GAGAGTGTCG AAGAGTTAAT GGACTTTTTC 
CAGGCTAGTA AGAGATACCA TCTCAGTAAA AGATTCGGCC ACAGAGAGTA CCTGGTCCAG 
AATATCCAGT CATCAGAGAC CCGTATGCAA GGCCTGCTGA GCACTGCGTC TGCCCAGCTG 
ACCCTCCTCA TCCAGAAACA CGAGAGAGCA GGCTACTTGG ATGAAGATCA GATGCAAGTA 
CTCTTGGAGC GGGCTCAAAC AGAAGTTTTT TCTATAAAAC AGAAGTTGGA CAATGACTTG 
AAGCAGGAAA AGAAAAAACT CCACCAAAAA CTAATAATTA AGAGAAGACG GGAGATGCTG 
CAAAAGCACA AGGAACAGCG GAGGGAGCAG CTATCCATCG CTGAAGCCTC TGGGGCTGCT 
GAGGATGCCG GCCAGTACCT GGGCCAGTGG CGGGGCCTGA TGGCTGAGCA CAGCGCCGCC 
CTGGAGGAGC TGCAGGAGCG CCTGGACCAG GCTGCCCTGG ACGAGCTCAG GGCCCTGACC 
CTCTCGCTGT CCGAGAAAGC CACGGAGGAG CTGCGGCGCC TGCAGAACTC AGGAATGACC 
CAGGAGCTAC TCAAGCGTGG GGTGCCTTGG CTCTTCCTGC AGCAGATCCT GGAGGAGCAC 
AGCAGGGACC TGGCAGCCAG GGCCGAGAGG CTGGAGGGCG AGGAGAGGGA CCGGGGCCAG 
GAGGGCGTCC AGAGCGTGAG GCAGAGACTG AAGGACGATG CGCTGGAGGC CTCCACGGAG 
GAGCAGGCGG AGCTGAGACA CTGGGAGCAC TTGATCTTCA CGAAGCTCTG CTCTTCTGCC 
TTCTCCCTGT CCGAAGAGGA GCTGCTTGGG ATGAGGCAGG AAGTTCACGG CTGCTTCGCT 
CAGATGGACA GGAGCTTGGC CCTCCCCAAA ATCCGGGCCC GAGTCCTGCT GCAACGATTC 
CAGACAGCTT GGCGAGAAGC AGAGTTCCTC AAACTGGACC AGGCCATGAC TGCCCCTGAG 
CTGCAGCCAC AGTCCAAGGC CAGAAAGCCA CGCTCCAAGA GCAGAAGCAA GATAGACCTT 
TTGAAGAAGT GCACTGAAGA TAAAATTCAG CTGTTCAAGG AACAGGCTCC CGAAGACCTG 
GTTGAAAAGG TCCGAGGAGA ATTGCTTCGG GAGAGAGTGC AACAGCTGGA GGCCCAGGAG 
GGACTCTTTG CCGAGTCCCT TGTCTCCCTG CAGTTCCAGA AGGCAGCGAG GATGGCCAGA 
ACCCTATGGG CGTACACCGC CCTCCTGAGC ATCCAGGACC TGCTTCTGGA GGAGCTGAAC 
TCATCAGAGA CCCTGACCAA GTCAGCCTGC ATGCAGATCC TGGAGTCTCA CAGCCCGGAG 
CTCCAGGAGC TGGAGAGAAA GCTGGAGGAC CAGCTGGCAC ACCAGGAGGC GGCCCAGCTG 
CAGCGGGCAC TGGACAGTTG GCAGCAATGG GCAGGTGAAG GACCTGCGCT TCTGCAGGAA 
CCAGAGGAGA CAGATTCTGA AAGGCACGTC TCCGCTGTCC TGCAGCGAGC CCTTAGCAAG 
GGTCAGAAGC TCCTGGAGTA TCACCAGCAG AGTTTGAGAG AGGAGCAAGA AGACAGTGTC 
GTGCTAGAAG ATTTGCTGGA GAATATGGAG ACTGACACCT TTGTGACCCT GTACGGCCAG 
GAGCTGAGAC TGGCGTCGTA CCTGTCGAAG CTGACGATGC TGCCGGGGGG CACGCTGCGC 
CGGCTCCTGA CCGTGGCTCT GCCCGCAGCC TCGCAGGCCG AGCTGCTGGC GGTGCTGGAC 
TCCGTGGGCC AGAAGCATCA AGACCACTCG GTGGAGAACG ATGGCAGCAG GGTGCAGGCT 
GACCTGGGCA GGAGGGGGAA ACACCAGGGT TGGTGGCAAG CCTTAGAGAG CAAACTTCGT 
GGAGAACTGA TAAACAGAGG ATTAGAGAAG ATGCTCTGGG CTCAGAAGAG GAAGGAGAGC 
ATATTAAAGA AGACGTGTCC CCCTCTCAGA GAGAGGGTGA TATTCTCTGG AAAAAGAAGT 
TGGCCGCACC TGTCCCTGGA GTCCACTGAT GAACTGACCC CAGTGCCCAT TGTAGGGGCA 
GAAGCTGTTG ACTTATTAAA CACGGGTGAA AAGCTCTTTA TCTTCAGAAA TCCAAAGGAG 
CCTGAGATCT CACTGCACGT TCCTCCCAGG AAGAAGAAGA AGAACTTTCT GAATGCCAAA 
AAGGCCGCGT GGGCCTTGGG CTTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848743.1
CDS	458..4087
Translation	MKFSKEIEVFNPPLASSASSGPWVHSVFAFTHSWPRKTLFKRDSAVTHRLYGDISRDFQGTSENGVIFQKCAVVSVQSEWPSAHVRLFVNNTRTPTAANLSDLFLLDNITGLTIRESAGNQTSRGFQAFRKKFLQVGDSFSVSYTASLEARDVGSGDILLLPAQLSFQSSSPNRTQLKAPFTITAEEKITVLPNHGLHAAGFCVAFILSLVLTWAVLFFMVRYQCVKGSSLTRHQVQHHENKLEHSQFTSADGVNEDLALNDQMIDILSSEDPGSMLQALEELEIATLNRADSDLEACRTQISKDIIALLLKNLTSSGQLSPQVERRMGAVFKKQFLLLEKEIQEEYDRKMVALTAECDLETRKKTESQYQREMAAMEEAEEVLKRVSERSAVECSSLLRTLHGLEQEHLRRSLALQQEEDLAKAHRQLAIFQRNELHNIFFTQIKSAIFKGELKPEAAKMLLQDYSKIQESVEELMDFFQASKRYHLSKRFGHREYLVQNIQSSETRMQGLLSTASAQLTLLIQKHERAGYLDEDQMQVLLERAQTEVFSIKQKLDNDLKQEKKKLHQKLIIKRRREMLQKHKEQRREQLSIAEASGAAEDAGQYLGQWRGLMAEHSAALEELQERLDQAALDELRALTLSLSEKATEELRRLQNSGMTQELLKRGVPWLFLQQILEEHSRDLAARAERLEGEERDRGQEGVQSVRQRLKDDALEASTEEQAELRHWEHLIFTKLCSSAFSLSEEELLGMRQEVHGCFAQMDRSLALPKIRARVLLQRFQTAWREAEFLKLDQAMTAPELQPQSKARKPRSKSRSKIDLLKKCTEDKIQLFKEQAPEDLVEKVRGELLRERVQQLEAQEGLFAESLVSLQFQKAARMARTLWAYTALLSIQDLLLEELNSSETLTKSACMQILESHSPELQELERKLEDQLAHQEAAQLQRALDSWQQWAGEGPALLQEPEETDSERHVSAVLQRALSKGQKLLEYHQQSLREEQEDSVVLEDLLENMETDTFVTLYGQELRLASYLSKLTMLPGGTLRRLLTVALPAASQAELLAVLDSVGQKHQDHSVENDGSRVQADLGRRGKHQGWWQALESKLRGELINRGLEKMLWAQKRKESILKKTCPPLRERVIFSGKRSWPHLSLESTDELTPVPIVGAEAVDLLNTGEKLFIFRNPKEPEISLHVPPRKKKKNFLNAKKAAWALGLN

Target ORF information:

RefSeq Version	XM_024992975.1
Organism	Bos taurus(cattle)
Definition	Bos taurus EvC ciliary complex subunit 2 (EVC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992975.1

ORF Insert Sequence:

ATGAAATTCA GTAAGGAAAT TGAAGTCTTT AACCCACCCC TGGCTTCTTC AGCCTCTAGT 
GGGCCATGGG TTCATTCCGT CTTTGCTTTT ACACACTCAT GGCCTAGGAA GACCTTGTTT 
AAAAGAGACT CTGCTGTAAC ACACCGCCTG TATGGAGACA TTTCAAGAGA CTTTCAAGGG 
ACTTCTGAGA ATGGAGTAAT TTTTCAGAAA TGTGCAGTGG TATCTGTGCA GAGTGAGTGG 
CCATCGGCCC ACGTGCGACT GTTTGTGAAC AACACCAGGA CACCCACGGC CGCCAACCTC 
TCCGACCTGT TCTTACTCGA CAACATCACA GGCCTCACGA TCAGGGAGTC GGCTGGAAAC 
CAGACCTCGA GAGGCTTCCA GGCTTTCAGG AAGAAGTTTC TGCAAGTTGG AGACTCCTTC 
TCCGTCAGCT ACACAGCCTC GCTGGAGGCC AGAGACGTCG GGAGTGGAGA CATCCTGCTG 
CTTCCTGCCC AGCTCTCCTT CCAGAGTTCG TCACCGAACA GAACCCAGCT AAAAGCCCCC 
TTCACCATAA CAGCAGAAGA AAAGATCACG GTTCTGCCAA ACCACGGCCT CCACGCAGCA 
GGGTTCTGCG TGGCCTTCAT CCTCTCCCTG GTGCTGACCT GGGCTGTGCT TTTTTTCATG 
GTTCGATACC AGTGTGTGAA GGGAAGCTCG CTTACCAGAC ATCAGGTTCA GCATCACGAA 
AACAAGCTGG AGCACTCCCA GTTCACCTCA GCTGATGGTG TGAATGAAGA TCTCGCTCTC 
AATGACCAAA TGATAGACAT TCTATCTTCT GAGGACCCCG GGAGCATGCT TCAAGCCTTA 
GAAGAGTTGG AAATTGCAAC CCTAAATCGG GCAGACTCAG ATCTGGAAGC TTGTCGAACT 
CAAATCAGCA AAGATATCAT TGCTCTTCTG CTGAAAAATC TGACCAGCAG TGGCCAGCTC 
TCCCCTCAGG TGGAGAGGAG GATGGGTGCT GTGTTCAAAA AGCAGTTTCT GTTGCTGGAG 
AAAGAAATTC AAGAGGAGTA TGATCGGAAG ATGGTGGCTC TGACAGCTGA GTGTGACCTG 
GAAACAAGAA AGAAGACAGA AAGCCAGTAC CAGAGGGAGA TGGCAGCCAT GGAGGAAGCA 
GAAGAGGTGC TGAAACGTGT TAGTGAGAGG TCCGCCGTCG AGTGCAGCAG CCTCCTGCGG 
ACCCTGCACG GCCTGGAGCA GGAACACCTG AGGAGGTCGC TCGCTCTACA GCAGGAGGAG 
GACCTTGCCA AGGCCCACAG GCAGCTGGCC ATTTTCCAGA GGAACGAACT GCACAATATC 
TTTTTTACCC AGATAAAAAG TGCTATTTTC AAAGGCGAGC TGAAGCCAGA GGCAGCAAAA 
ATGCTGCTGC AAGATTACTC TAAAATACAG GAGAGTGTCG AAGAGTTAAT GGACTTTTTC 
CAGGCTAGTA AGAGATACCA TCTCAGTAAA AGATTCGGCC ACAGAGAGTA CCTGGTCCAG 
AATATCCAGT CATCAGAGAC CCGTATGCAA GGCCTGCTGA GCACTGCGTC TGCCCAGCTG 
ACCCTCCTCA TCCAGAAACA CGAGAGAGCA GGCTACTTGG ATGAAGATCA GATGCAAGTA 
CTCTTGGAGC GGGCTCAAAC AGAAGTTTTT TCTATAAAAC AGAAGTTGGA CAATGACTTG 
AAGCAGGAAA AGAAAAAACT CCACCAAAAA CTAATAATTA AGAGAAGACG GGAGATGCTG 
CAAAAGCACA AGGAACAGCG GAGGGAGCAG CTATCCATCG CTGAAGCCTC TGGGGCTGCT 
GAGGATGCCG GCCAGTACCT GGGCCAGTGG CGGGGCCTGA TGGCTGAGCA CAGCGCCGCC 
CTGGAGGAGC TGCAGGAGCG CCTGGACCAG GCTGCCCTGG ACGAGCTCAG GGCCCTGACC 
CTCTCGCTGT CCGAGAAAGC CACGGAGGAG CTGCGGCGCC TGCAGAACTC AGGAATGACC 
CAGGAGCTAC TCAAGCGTGG GGTGCCTTGG CTCTTCCTGC AGCAGATCCT GGAGGAGCAC 
AGCAGGGACC TGGCAGCCAG GGCCGAGAGG CTGGAGGGCG AGGAGAGGGA CCGGGGCCAG 
GAGGGCGTCC AGAGCGTGAG GCAGAGACTG AAGGACGATG CGCTGGAGGC CTCCACGGAG 
GAGCAGGCGG AGCTGAGACA CTGGGAGCAC TTGATCTTCA CGAAGCTCTG CTCTTCTGCC 
TTCTCCCTGT CCGAAGAGGA GCTGCTTGGG ATGAGGCAGG AAGTTCACGG CTGCTTCGCT 
CAGATGGACA GGAGCTTGGC CCTCCCCAAA ATCCGGGCCC GAGTCCTGCT GCAACGATTC 
CAGACAGCTT GGCGAGAAGC AGAGTTCCTC AAACTGGACC AGGCCATGAC TGCCCCTGAG 
CTGCAGCCAC AGTCCAAGGC CAGAAAGCCA CGCTCCAAGA GCAGAAGCAA GATAGACCTT 
TTGAAGAAGT GCACTGAAGA TAAAATTCAG CTGTTCAAGG AACAGGCTCC CGAAGACCTG 
GTTGAAAAGG TCCGAGGAGA ATTGCTTCGG GAGAGAGTGC AACAGCTGGA GGCCCAGGAG 
GGACTCTTTG CCGAGTCCCT TGTCTCCCTG CAGTTCCAGA AGGCAGCGAG GATGGCCAGA 
ACCCTATGGG CGTACACCGC CCTCCTGAGC ATCCAGGACC TGCTTCTGGA GGAGCTGAAC 
TCATCAGAGA CCCTGACCAA GTCAGCCTGC ATGCAGATCC TGGAGTCTCA CAGCCCGGAG 
CTCCAGGAGC TGGAGAGAAA GCTGGAGGAC CAGCTGGCAC ACCAGGAGGC GGCCCAGCTG 
CAGCGGGCAC TGGACAGTTG GCAGCAATGG GCAGGTGAAG GACCTGCGCT TCTGCAGGAA 
CCAGAGGAGA CAGATTCTGA AAGGCACGTC TCCGCTGTCC TGCAGCGAGC CCTTAGCAAG 
GGTCAGAAGC TCCTGGAGTA TCACCAGCAG AGTTTGAGAG AGGAGCAAGA AGACAGTGTC 
GTGCTAGAAG ATTTGCTGGA GAATATGGAG ACTGACACCT TTGTGACCCT GTACGGCCAG 
GAGCTGAGAC TGGCGTCGTA CCTGTCGAAG CTGACGATGC TGCCGGGGGG CACGCTGCGC 
CGGCTCCTGA CCGTGGCTCT GCCCGCAGCC TCGCAGGCCG AGCTGCTGGC GGTGCTGGAC 
TCCGTGGGCC AGAAGCATCA AGACCACTCG GTGGAGAACG ATGGCAGCAG GGTGCAGGCT 
GACCTGGGCA GGAGGGGGAA ACACCAGGGT TGGTGGCAAG CCTTAGAGAG CAAACTTCGT 
GGAGAACTGA TAAACAGAGG ATTAGAGAAG ATGCTCTGGG CTCAGAAGAG GAAGGAGAGC 
ATATTAAAGA AGACGTGTCC CCCTCTCAGA GAGAGGGTGA TATTCTCTGG AAAAAGAAGT 
TGGCCGCACC TGTCCCTGGA GTCCACTGAT GAACTGACCC CAGTGCCCAT TGTAGGGGCA 
GAAGCTGTTG ACTTATTAAA CACGGGTGAA AAGCTCTTTA TCTTCAGAAA TCCAAAGGAG 
CCTGAGATCT CACTGCACGT TCCTCCCAGG AAGAAGAAGA AGAACTTTCT GAATGCCAAA 
AAGGCCGCGT GGGCCTTGGG CTTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion in the EVC2 gene causes chondrodysplastic dwarfism in Tyrolean Grey cattle.
PloS one9(4)e94861(2014)
Murgiano L,Jagannathan V,Benazzi C,Bolcato M,Brunetti B,Muscatello LV,Dittmer K,Piffer C,Gentile A,Dr?gem?ller C

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Positional cloning of the gene LIMBIN responsible for bovine chondrodysplastic dwarfism.
Proceedings of the National Academy of Sciences of the United States of America99(16)10549-54(2002 Aug)
Takeda H,Takami M,Oguni T,Tsuji T,Yoneda K,Sato H,Ihara N,Itoh T,Kata SR,Mishina Y,Womack JE,Moritomo Y,Sugimoto Y,Kunieda T

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EVC2 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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