GNAT1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	GNAT1
Entrez Gene ID	281794
Full Name	G protein subunit alpha transducin 1
General protein information	Preferred Names G protein subunit alpha transducin 1 Names guanine nucleotide-binding protein G(t) subunit alpha-1 guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1 transducin alpha-1 chain
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010817664.3	XP_010815966.2	guanine nucleotide-binding protein G(t) subunit alpha-1 isoform X1
NM_181022.2	NP_851365.1	guanine nucleotide-binding protein G(t) subunit alpha-1
XM_010817664.2	XP_010815966.1	guanine nucleotide-binding protein G(t) subunit alpha-1 isoform X1
XM_024982389.1	XP_024838157.1	guanine nucleotide-binding protein G(t) subunit alpha-1 isoform X2
XM_024982388.1	XP_024838156.1	guanine nucleotide-binding protein G(t) subunit alpha-1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAT1	XP_001167971.1
Canis lupus familiaris (dog)	GNAT1	NP_001003068.1
Mus musculus (house mouse)	Gnat1	NP_032166.1
Rattus norvegicus (Norway rat)	Gnat1	NP_001102250.2
Gallus gallus (chicken)	GNAT1	NP_990022.1
Xenopus tropicalis (tropical clawed frog)	gnat1	NP_001096278.1
Homo sapiens (human)	GNAT1	NP_653082.1
	GNAT1	XP_001103202.1
Bos taurus (cattle)	GNAT1	NP_851365.1
Danio rerio (zebrafish)	gnat1	NP_571943.1

Gene Ontology
Bibliography

Related articles in PubMed

Isolation and structure-function characterization of a signaling-active rhodopsin-G protein complex.
Gao Y, Westfield G, Erickson JW, Cerione RA, Skiniotis G, Ramachandran S
The Journal of biological chemistry292(34)14280-14289(2017 08)

Rod visual pigment optimizes active state to achieve efficient G protein activation as compared with cone visual pigments.
Kojima K, Imamoto Y, Maeda R, Yamashita T, Shichida Y
The Journal of biological chemistry289(8)5061-73(2014 Feb)

Insights into congenital stationary night blindness based on the structure of G90D rhodopsin.
Singhal A, Ostermaier MK, Vishnivetskiy SA, Panneels V, Homan KT, Tesmer JJ, Veprintsev D, Deupi X, Gurevich VV, Schertler GF, Standfuss J
EMBO reports14(6)520-6(2013 Jun)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Subunit dissociation and diffusion determine the subcellular localization of rod and cone transducins.
Rosenzweig DH, Nair KS, Wei J, Wang Q, Garwin G, Saari JC, Chen CK, Smrcka AV, Swaroop A, Lem J, Hurley JB, Slepak VZ
The Journal of neuroscience : the official journal of the Society for Neuroscience27(20)5484-94(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that a hetero-multimer complex forms between light-activated rhodopsin and light-activated heterotrimeric transducin (T-alpha-1, Gnb1, Gngt1); the stoichiometry is 1:1 rhodopsin:transducin. The complex appears to form on native rod outer segment membranes upon light activation.
Title: Isolation and structure-function characterization of a signaling-active rhodopsin-G protein complex.

These results indicated that efficient Gt activation by rhodopsin, resulting from an optimized active state of rhodopsin, is one of the causes of the high amplification efficiency of rods.
Title: Rod visual pigment optimizes active state to achieve efficient G protein activation as compared with cone visual pigments.

The following GNAT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01451	NM_181022.2 Latest version!	Bos taurus G protein subunit alpha transducin 1 (GNAT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa45237	XM_010817664.2 Latest version!	Bos taurus guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1 (GNAT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa45237	XM_024982389.1 Latest version!	Bos taurus G protein subunit alpha transducin 1 (GNAT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa228728	XM_024982388.1 Latest version!	Bos taurus G protein subunit alpha transducin 1 (GNAT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa228728	XM_010817664.3 Latest version!	Bos taurus G protein subunit alpha transducin 1 (GNAT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNAT1 ( NM_181022.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_851365.1 Bos taurus G protein subunit alpha transducin 1 (GNAT1), mRNA.

Price & Availability↑

CloneID	OBa01451
Clone ID Related Accession (Same CDS sequence)	NM_181022.2
Accession Version	NM_181022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(t) subunit alpha-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X02440.1. On Jun 3, 2003 this sequence version replaced NM_181022.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X02440.1, K03253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGCTG GGGCCAGCGC TGAGGAGAAG CACTCAAGGG AGCTGGAAAA GAAGCTGAAA 
GAAGATGCTG AGAAAGATGC TCGAACCGTG AAACTGCTGC TTCTGGGTGC CGGTGAATCC 
GGGAAGAGTA CCATTGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCACTGGAA 
GAGTGTCTTG AGTTCATTGC CATCATCTAT GGCAACACGC TACAGTCCAT CCTGGCCATT 
GTGCGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCGCGCCA GGACGACGCC 
CGAAAGCTGA TGCACATGGC AGACACCATC GAGGAGGGCA CGATGCCCAA GGAGATGTCA 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTCGA CCGAGCCTCA 
GAGTACCAGC TCAACGACTC TGCTGGCTAC TATCTCTCAG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACTGA ACAGGATGTG CTGCGCTCCC GTGTCAAGAC CACGGGTATC 
ATTGAGACGC AGTTCTCCTT CAAGGACCTC AACTTTCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCAGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGGG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTG GTGGAAGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGTATCTGC AACCACCGCT ACTTCGCCAC CACGTCCATC 
GTGCTCTTTC TCAACAAGAA GGACGTCTTC TCGGAGAAGA TCAAAAAGGC GCACCTTAGT 
ATCTGCTTTC CGGACTACAA CGGGCCCAAC ACGTATGAGG ACGCCGGCAA TTACATCAAG 
GTGCAATTCC TTGAGCTCAA CATGCGACGC GACGTGAAGG AGATCTATTC CCACATGACA 
TGCGCCACCG ACACGCAGAA CGTCAAGTTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG CGGGCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851365.1
CDS	138..1190
Translation	MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFDRASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	NM_181022.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein subunit alpha transducin 1 (GNAT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181022.2

ORF Insert Sequence:

ATGGGGGCTG GGGCCAGCGC TGAGGAGAAG CACTCAAGGG AGCTGGAAAA GAAGCTGAAA 
GAAGATGCTG AGAAAGATGC TCGAACCGTG AAACTGCTGC TTCTGGGTGC CGGTGAATCC 
GGGAAGAGTA CCATTGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCACTGGAA 
GAGTGTCTTG AGTTCATTGC CATCATCTAT GGCAACACGC TACAGTCCAT CCTGGCCATT 
GTGCGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCGCGCCA GGACGACGCC 
CGAAAGCTGA TGCACATGGC AGACACCATC GAGGAGGGCA CGATGCCCAA GGAGATGTCA 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTCGA CCGAGCCTCA 
GAGTACCAGC TCAACGACTC TGCTGGCTAC TATCTCTCAG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACTGA ACAGGATGTG CTGCGCTCCC GTGTCAAGAC CACGGGTATC 
ATTGAGACGC AGTTCTCCTT CAAGGACCTC AACTTTCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCAGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGGG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTG GTGGAAGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGTATCTGC AACCACCGCT ACTTCGCCAC CACGTCCATC 
GTGCTCTTTC TCAACAAGAA GGACGTCTTC TCGGAGAAGA TCAAAAAGGC GCACCTTAGT 
ATCTGCTTTC CGGACTACAA CGGGCCCAAC ACGTATGAGG ACGCCGGCAA TTACATCAAG 
GTGCAATTCC TTGAGCTCAA CATGCGACGC GACGTGAAGG AGATCTATTC CCACATGACA 
TGCGCCACCG ACACGCAGAA CGTCAAGTTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG CGGGCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT1 ( XM_010817664.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815966.1 Bos taurus guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1 (GNAT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45237
Clone ID Related Accession (Same CDS sequence)	XM_010817664.2 , XM_024982389.1
Accession Version	XM_010817664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(t) subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCTG GGGCCAGCGC TGAGGAGAAG CACTCAAGGG AGCTGGAAAA GAAGCTGAAA 
GAAGATGCTG AGAAAGATGC TCGAACCGTG AAACTGCTGC TTCTGGGTGC CGGTGAATCT 
GGGAAGAGTA CCATTGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCACTGGAA 
GAGTGTCTTG AGTTCATTGC CATCATCTAT GGCAACACGC TACAGTCCAT CCTGGCCATT 
GTGCGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCGCGCCA GGACGACGCC 
CGAAAGCTGA TGCACATGGC AGACACCATC GAGGAGGGCA CGATGCCCAA GGAGATGTCA 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTCGA CCGAGCCTCA 
GAGTACCAGC TCAACGACTC TGCTGGCTAC TATCTCTCAG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACTGA ACAGGATGTG CTGCGCTCCC GTGTCAAGAC CACGGGTATC 
ATTGAGACGC AGTTCTCCTT CAAGGACCTC AACTTTCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCAGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGGG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTG GTGGAAGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGTATCTGC AACCACCGCT ACTTCGCCAC CACGTCCATC 
GTGCTCTTTC TCAACAAGAA GGACGTCTTC TCGGAGAAGA TCAAAAAGGC GCACCTTAGC 
ATCTGCTTTC CGGACTACAA CGGGCCCAAC ACGTATGAGG ACGCCGGCAA TTACATCAAG 
GTGCAATTCC TTGAGCTCAA CATGCGACGC GACGTGAAGG AGATCTATTC CCACATGACA 
TGCGCCACCG ACACGCAGAA CGTCAAGTTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG CGGGCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815966.1
CDS	157..1209
Translation	MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFDRASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	XM_010817664.2
Organism	Bos taurus(cattle)
Definition	Bos taurus guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1 (GNAT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817664.2

ORF Insert Sequence:

ATGGGGGCTG GGGCCAGCGC TGAGGAGAAG CACTCAAGGG AGCTGGAAAA GAAGCTGAAA 
GAAGATGCTG AGAAAGATGC TCGAACCGTG AAACTGCTGC TTCTGGGTGC CGGTGAATCT 
GGGAAGAGTA CCATTGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCACTGGAA 
GAGTGTCTTG AGTTCATTGC CATCATCTAT GGCAACACGC TACAGTCCAT CCTGGCCATT 
GTGCGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCGCGCCA GGACGACGCC 
CGAAAGCTGA TGCACATGGC AGACACCATC GAGGAGGGCA CGATGCCCAA GGAGATGTCA 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTCGA CCGAGCCTCA 
GAGTACCAGC TCAACGACTC TGCTGGCTAC TATCTCTCAG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACTGA ACAGGATGTG CTGCGCTCCC GTGTCAAGAC CACGGGTATC 
ATTGAGACGC AGTTCTCCTT CAAGGACCTC AACTTTCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCAGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGGG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTG GTGGAAGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGTATCTGC AACCACCGCT ACTTCGCCAC CACGTCCATC 
GTGCTCTTTC TCAACAAGAA GGACGTCTTC TCGGAGAAGA TCAAAAAGGC GCACCTTAGC 
ATCTGCTTTC CGGACTACAA CGGGCCCAAC ACGTATGAGG ACGCCGGCAA TTACATCAAG 
GTGCAATTCC TTGAGCTCAA CATGCGACGC GACGTGAAGG AGATCTATTC CCACATGACA 
TGCGCCACCG ACACGCAGAA CGTCAAGTTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG CGGGCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT1 ( XM_024982389.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838157.1 Bos taurus G protein subunit alpha transducin 1 (GNAT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa45237
Clone ID Related Accession (Same CDS sequence)	XM_010817664.2 , XM_024982389.1
Accession Version	XM_024982389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(t) subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCTG GGGCCAGCGC TGAGGAGAAG CACTCAAGGG AGCTGGAAAA GAAGCTGAAA 
GAAGATGCTG AGAAAGATGC TCGAACCGTG AAACTGCTGC TTCTGGGTGC CGGTGAATCT 
GGGAAGAGTA CCATTGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCACTGGAA 
GAGTGTCTTG AGTTCATTGC CATCATCTAT GGCAACACGC TACAGTCCAT CCTGGCCATT 
GTGCGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCGCGCCA GGACGACGCC 
CGAAAGCTGA TGCACATGGC AGACACCATC GAGGAGGGCA CGATGCCCAA GGAGATGTCA 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTCGA CCGAGCCTCA 
GAGTACCAGC TCAACGACTC TGCTGGCTAC TATCTCTCAG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACTGA ACAGGATGTG CTGCGCTCCC GTGTCAAGAC CACGGGTATC 
ATTGAGACGC AGTTCTCCTT CAAGGACCTC AACTTTCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCAGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGGG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTG GTGGAAGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGTATCTGC AACCACCGCT ACTTCGCCAC CACGTCCATC 
GTGCTCTTTC TCAACAAGAA GGACGTCTTC TCGGAGAAGA TCAAAAAGGC GCACCTTAGC 
ATCTGCTTTC CGGACTACAA CGGGCCCAAC ACGTATGAGG ACGCCGGCAA TTACATCAAG 
GTGCAATTCC TTGAGCTCAA CATGCGACGC GACGTGAAGG AGATCTATTC CCACATGACA 
TGCGCCACCG ACACGCAGAA CGTCAAGTTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG CGGGCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838157.1
CDS	1317..2369
Translation	MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFDRASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	XM_024982389.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein subunit alpha transducin 1 (GNAT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982389.1

ORF Insert Sequence:

ATGGGGGCTG GGGCCAGCGC TGAGGAGAAG CACTCAAGGG AGCTGGAAAA GAAGCTGAAA 
GAAGATGCTG AGAAAGATGC TCGAACCGTG AAACTGCTGC TTCTGGGTGC CGGTGAATCT 
GGGAAGAGTA CCATTGTCAA GCAGATGAAG ATTATCCACC AGGACGGGTA CTCACTGGAA 
GAGTGTCTTG AGTTCATTGC CATCATCTAT GGCAACACGC TACAGTCCAT CCTGGCCATT 
GTGCGCGCCA TGACCACACT CAACATCCAG TACGGAGACT CTGCGCGCCA GGACGACGCC 
CGAAAGCTGA TGCACATGGC AGACACCATC GAGGAGGGCA CGATGCCCAA GGAGATGTCA 
GACATCATCC AGCGGCTGTG GAAGGACTCC GGTATCCAGG CCTGTTTCGA CCGAGCCTCA 
GAGTACCAGC TCAACGACTC TGCTGGCTAC TATCTCTCAG ACCTGGAGCG CCTGGTAACC 
CCGGGCTACG TGCCCACTGA ACAGGATGTG CTGCGCTCCC GTGTCAAGAC CACGGGTATC 
ATTGAGACGC AGTTCTCCTT CAAGGACCTC AACTTTCGGA TGTTCGATGT GGGCGGGCAG 
CGCTCAGAGC GCAAGAAGTG GATCCACTGC TTCGAGGGGG TGACCTGCAT CATCTTCATC 
GCGGCGCTGA GCGCCTACGA CATGGTGCTG GTGGAAGACG ACGAAGTGAA CCGCATGCAC 
GAGAGCCTGC ACCTGTTCAA CAGTATCTGC AACCACCGCT ACTTCGCCAC CACGTCCATC 
GTGCTCTTTC TCAACAAGAA GGACGTCTTC TCGGAGAAGA TCAAAAAGGC GCACCTTAGC 
ATCTGCTTTC CGGACTACAA CGGGCCCAAC ACGTATGAGG ACGCCGGCAA TTACATCAAG 
GTGCAATTCC TTGAGCTCAA CATGCGACGC GACGTGAAGG AGATCTATTC CCACATGACA 
TGCGCCACCG ACACGCAGAA CGTCAAGTTT GTCTTCGACG CTGTCACCGA CATCATCATC 
AAGGAGAACC TCAAAGACTG CGGGCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT1 ( XM_010817664.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815966.2 Bos taurus G protein subunit alpha transducin 1 (GNAT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa228728
Clone ID Related Accession (Same CDS sequence)	XM_010817664.3 , XM_024982388.1
Accession Version	XM_010817664.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(t) subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817664.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGC CACCAGCTCC CAGCTCCCAG AACCAGTGTC TACTTCCTAC ATGTTTAGGG 
ACCATGGGGG CTGGGGCCAG CGCTGAGGAG AAGCACTCAA GGGAGCTGGA AAAGAAGCTG 
AAAGAAGATG CTGAGAAAGA TGCTCGAACC GTGAAACTGC TGCTTCTGGG TGCCGGTGAA 
TCTGGGAAGA GTACCATTGT CAAGCAGATG AAGATTATCC ACCAGGACGG GTACTCACTG 
GAAGAGTGTC TTGAGTTCAT TGCCATCATC TATGGCAACA CGCTACAGTC CATCCTGGCC 
ATTGTGCGCG CCATGACCAC ACTCAACATC CAGTACGGAG ACTCTGCGCG CCAGGACGAC 
GCCCGAAAGC TGATGCACAT GGCAGACACC ATCGAGGAGG GCACGATGCC CAAGGAGATG 
TCAGACATCA TCCAGCGGCT GTGGAAGGAC TCCGGTATCC AGGCCTGTTT CGACCGAGCC 
TCAGAGTACC AGCTCAACGA CTCTGCTGGC TACTATCTCT CAGACCTGGA GCGCCTGGTA 
ACCCCGGGCT ACGTGCCCAC TGAACAGGAT GTGCTGCGCT CCCGTGTCAA GACCACGGGT 
ATCATTGAGA CGCAGTTCTC CTTCAAGGAC CTCAACTTTC GGATGTTCGA TGTGGGCGGG 
CAGCGCTCAG AGCGCAAGAA GTGGATCCAC TGCTTCGAGG GGGTGACCTG CATCATCTTC 
ATCGCGGCGC TGAGCGCCTA CGACATGGTG CTGGTGGAAG ACGACGAAGT GAACCGCATG 
CACGAGAGCC TGCACCTGTT CAACAGTATC TGCAACCACC GCTACTTCGC CACCACGTCC 
ATCGTGCTCT TTCTCAACAA GAAGGACGTC TTCTCGGAGA AGATCAAAAA GGCGCACCTT 
AGCATCTGCT TTCCGGACTA CAACGGGCCC AACACGTATG AGGACGCCGG CAATTACATC 
AAGGTGCAAT TCCTTGAGCT CAACATGCGA CGCGACGTGA AGGAGATCTA TTCCCACATG 
ACATGCGCCA CCGACACGCA GAACGTCAAG TTTGTCTTCG ACGCTGTCAC CGACATCATC 
ATCAAGGAGA ACCTCAAAGA CTGCGGGCTC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815966.2
CDS	402..1517
Translation	MLKPPAPSSQNQCLLPTCLGTMGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFDRASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	XM_010817664.3
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein subunit alpha transducin 1 (GNAT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817664.3

ORF Insert Sequence:

ATGTTGAAGC CACCAGCTCC CAGCTCCCAG AACCAGTGTC TACTTCCTAC ATGTTTAGGG 
ACCATGGGGG CTGGGGCCAG CGCTGAGGAG AAGCACTCAA GGGAGCTGGA AAAGAAGCTG 
AAAGAAGATG CTGAGAAAGA TGCTCGAACC GTGAAACTGC TGCTTCTGGG TGCCGGTGAA 
TCTGGGAAGA GTACCATTGT CAAGCAGATG AAGATTATCC ACCAGGACGG GTACTCACTG 
GAAGAGTGTC TTGAGTTCAT TGCCATCATC TATGGCAACA CGCTACAGTC CATCCTGGCC 
ATTGTGCGCG CCATGACCAC ACTCAACATC CAGTACGGAG ACTCTGCGCG CCAGGACGAC 
GCCCGAAAGC TGATGCACAT GGCAGACACC ATCGAGGAGG GCACGATGCC CAAGGAGATG 
TCAGACATCA TCCAGCGGCT GTGGAAGGAC TCCGGTATCC AGGCCTGTTT CGACCGAGCC 
TCAGAGTACC AGCTCAACGA CTCTGCTGGC TACTATCTCT CAGACCTGGA GCGCCTGGTA 
ACCCCGGGCT ACGTGCCCAC TGAACAGGAT GTGCTGCGCT CCCGTGTCAA GACCACGGGT 
ATCATTGAGA CGCAGTTCTC CTTCAAGGAC CTCAACTTTC GGATGTTCGA TGTGGGCGGG 
CAGCGCTCAG AGCGCAAGAA GTGGATCCAC TGCTTCGAGG GGGTGACCTG CATCATCTTC 
ATCGCGGCGC TGAGCGCCTA CGACATGGTG CTGGTGGAAG ACGACGAAGT GAACCGCATG 
CACGAGAGCC TGCACCTGTT CAACAGTATC TGCAACCACC GCTACTTCGC CACCACGTCC 
ATCGTGCTCT TTCTCAACAA GAAGGACGTC TTCTCGGAGA AGATCAAAAA GGCGCACCTT 
AGCATCTGCT TTCCGGACTA CAACGGGCCC AACACGTATG AGGACGCCGG CAATTACATC 
AAGGTGCAAT TCCTTGAGCT CAACATGCGA CGCGACGTGA AGGAGATCTA TTCCCACATG 
ACATGCGCCA CCGACACGCA GAACGTCAAG TTTGTCTTCG ACGCTGTCAC CGACATCATC 
ATCAAGGAGA ACCTCAAAGA CTGCGGGCTC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAT1 ( XM_024982388.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838156.1 Bos taurus G protein subunit alpha transducin 1 (GNAT1), transcript variant X1, mRNA.

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CloneID	OBa228728
Clone ID Related Accession (Same CDS sequence)	XM_010817664.3 , XM_024982388.1
Accession Version	XM_024982388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(t) subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGC CACCAGCTCC CAGCTCCCAG AACCAGTGTC TACTTCCTAC ATGTTTAGGG 
ACCATGGGGG CTGGGGCCAG CGCTGAGGAG AAGCACTCAA GGGAGCTGGA AAAGAAGCTG 
AAAGAAGATG CTGAGAAAGA TGCTCGAACC GTGAAACTGC TGCTTCTGGG TGCCGGTGAA 
TCTGGGAAGA GTACCATTGT CAAGCAGATG AAGATTATCC ACCAGGACGG GTACTCACTG 
GAAGAGTGTC TTGAGTTCAT TGCCATCATC TATGGCAACA CGCTACAGTC CATCCTGGCC 
ATTGTGCGCG CCATGACCAC ACTCAACATC CAGTACGGAG ACTCTGCGCG CCAGGACGAC 
GCCCGAAAGC TGATGCACAT GGCAGACACC ATCGAGGAGG GCACGATGCC CAAGGAGATG 
TCAGACATCA TCCAGCGGCT GTGGAAGGAC TCCGGTATCC AGGCCTGTTT CGACCGAGCC 
TCAGAGTACC AGCTCAACGA CTCTGCTGGC TACTATCTCT CAGACCTGGA GCGCCTGGTA 
ACCCCGGGCT ACGTGCCCAC TGAACAGGAT GTGCTGCGCT CCCGTGTCAA GACCACGGGT 
ATCATTGAGA CGCAGTTCTC CTTCAAGGAC CTCAACTTTC GGATGTTCGA TGTGGGCGGG 
CAGCGCTCAG AGCGCAAGAA GTGGATCCAC TGCTTCGAGG GGGTGACCTG CATCATCTTC 
ATCGCGGCGC TGAGCGCCTA CGACATGGTG CTGGTGGAAG ACGACGAAGT GAACCGCATG 
CACGAGAGCC TGCACCTGTT CAACAGTATC TGCAACCACC GCTACTTCGC CACCACGTCC 
ATCGTGCTCT TTCTCAACAA GAAGGACGTC TTCTCGGAGA AGATCAAAAA GGCGCACCTT 
AGCATCTGCT TTCCGGACTA CAACGGGCCC AACACGTATG AGGACGCCGG CAATTACATC 
AAGGTGCAAT TCCTTGAGCT CAACATGCGA CGCGACGTGA AGGAGATCTA TTCCCACATG 
ACATGCGCCA CCGACACGCA GAACGTCAAG TTTGTCTTCG ACGCTGTCAC CGACATCATC 
ATCAAGGAGA ACCTCAAAGA CTGCGGGCTC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838156.1
CDS	402..1517
Translation	MLKPPAPSSQNQCLLPTCLGTMGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFDRASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Target ORF information:

RefSeq Version	XM_024982388.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein subunit alpha transducin 1 (GNAT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982388.1

ORF Insert Sequence:

ATGTTGAAGC CACCAGCTCC CAGCTCCCAG AACCAGTGTC TACTTCCTAC ATGTTTAGGG 
ACCATGGGGG CTGGGGCCAG CGCTGAGGAG AAGCACTCAA GGGAGCTGGA AAAGAAGCTG 
AAAGAAGATG CTGAGAAAGA TGCTCGAACC GTGAAACTGC TGCTTCTGGG TGCCGGTGAA 
TCTGGGAAGA GTACCATTGT CAAGCAGATG AAGATTATCC ACCAGGACGG GTACTCACTG 
GAAGAGTGTC TTGAGTTCAT TGCCATCATC TATGGCAACA CGCTACAGTC CATCCTGGCC 
ATTGTGCGCG CCATGACCAC ACTCAACATC CAGTACGGAG ACTCTGCGCG CCAGGACGAC 
GCCCGAAAGC TGATGCACAT GGCAGACACC ATCGAGGAGG GCACGATGCC CAAGGAGATG 
TCAGACATCA TCCAGCGGCT GTGGAAGGAC TCCGGTATCC AGGCCTGTTT CGACCGAGCC 
TCAGAGTACC AGCTCAACGA CTCTGCTGGC TACTATCTCT CAGACCTGGA GCGCCTGGTA 
ACCCCGGGCT ACGTGCCCAC TGAACAGGAT GTGCTGCGCT CCCGTGTCAA GACCACGGGT 
ATCATTGAGA CGCAGTTCTC CTTCAAGGAC CTCAACTTTC GGATGTTCGA TGTGGGCGGG 
CAGCGCTCAG AGCGCAAGAA GTGGATCCAC TGCTTCGAGG GGGTGACCTG CATCATCTTC 
ATCGCGGCGC TGAGCGCCTA CGACATGGTG CTGGTGGAAG ACGACGAAGT GAACCGCATG 
CACGAGAGCC TGCACCTGTT CAACAGTATC TGCAACCACC GCTACTTCGC CACCACGTCC 
ATCGTGCTCT TTCTCAACAA GAAGGACGTC TTCTCGGAGA AGATCAAAAA GGCGCACCTT 
AGCATCTGCT TTCCGGACTA CAACGGGCCC AACACGTATG AGGACGCCGG CAATTACATC 
AAGGTGCAAT TCCTTGAGCT CAACATGCGA CGCGACGTGA AGGAGATCTA TTCCCACATG 
ACATGCGCCA CCGACACGCA GAACGTCAAG TTTGTCTTCG ACGCTGTCAC CGACATCATC 
ATCAAGGAGA ACCTCAAAGA CTGCGGGCTC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Isolation and structure-function characterization of a signaling-active rhodopsin-G protein complex.
The Journal of biological chemistry292(34)14280-14289(2017 08)
Gao Y,Westfield G,Erickson JW,Cerione RA,Skiniotis G,Ramachandran S

Rod visual pigment optimizes active state to achieve efficient G protein activation as compared with cone visual pigments.
The Journal of biological chemistry289(8)5061-73(2014 Feb)
Kojima K,Imamoto Y,Maeda R,Yamashita T,Shichida Y

Insights into congenital stationary night blindness based on the structure of G90D rhodopsin.
EMBO reports14(6)520-6(2013 Jun)
Singhal A,Ostermaier MK,Vishnivetskiy SA,Panneels V,Homan KT,Tesmer JJ,Veprintsev D,Deupi X,Gurevich VV,Schertler GF,Standfuss J

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Subunit dissociation and diffusion determine the subcellular localization of rod and cone transducins.
The Journal of neuroscience : the official journal of the Society for Neuroscience27(20)5484-94(2007 May)
Rosenzweig DH,Nair KS,Wei J,Wang Q,Garwin G,Saari JC,Chen CK,Smrcka AV,Swaroop A,Lem J,Hurley JB,Slepak VZ

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GNAT1 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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