WNK1 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following WNK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa234910 XM_024992710.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234911 XM_024992711.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234912 XM_024992712.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234913 XM_024992713.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234914 XM_024992714.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234915 XM_024992715.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234916 XM_024992717.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234917 XM_024992718.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234918 XM_024992719.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234919 XM_024992720.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234920 XM_024992721.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234921 XM_024992722.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa234922 XM_024992723.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234923 XM_024992724.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234924 XM_024992725.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234925 XM_024992727.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234926 XM_024992728.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234927 XM_024992729.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234928 XM_024992730.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234929 XM_024992731.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234930 XM_024992732.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa89985 XM_015471356.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa89985 XM_015463540.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa89988 XM_015471359.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa89988 XM_015463543.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa19898 XM_005207280.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa19898 XM_015463526.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa19899 XM_005207281.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa19899 XM_015463527.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa19911 XM_015463544.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa19911 XM_005207292.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa19912 XM_015463546.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa19912 XM_005207293.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa19913 XM_005207294.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa19913 XM_015463547.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa19914 XM_015463548.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa19914 XM_005207295.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa46553 XM_010805674.2
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa46553 XM_015463539.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa46554 XM_015463545.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa46554 XM_010805675.2
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa46555 XM_015463549.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa46555 XM_010805676.2
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa46556 XM_015463550.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa46556 XM_010805677.2
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa46557 XM_010805678.2
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa46557 XM_015463551.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa46558 XM_015463552.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa46558 XM_010805679.2
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa46559 XM_015463553.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa46559 XM_010805680.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa46560 XM_010805681.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa46560 XM_015463554.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa46561 XM_010805682.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa46561 XM_015463555.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa89974 XM_015471345.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89974 XM_015463528.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89975 XM_015471346.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89975 XM_015463529.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89976 XM_015471347.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89976 XM_015463530.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89977 XM_015463531.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89977 XM_015471348.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89978 XM_015471349.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89978 XM_015463532.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89979 XM_015463533.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89979 XM_015471350.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89980 XM_015471351.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89980 XM_015463534.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89981 XM_015463535.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89981 XM_015471352.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89982 XM_015471353.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89982 XM_015463536.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89983 XM_015471354.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89983 XM_015463537.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89984 XM_015471355.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89984 XM_015463538.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89986 XM_015471357.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa89986 XM_015463541.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa89987 XM_015471358.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa89987 XM_015463542.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
View Old Accession Versions >>
OBa46559 XM_010805680.2 Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa46560 XM_010805681.2 Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa46561 XM_010805682.2 Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa234910
    Clone ID Related Accession (Same CDS sequence) XM_024992710.1
    Accession Version XM_024992710.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGCT GTGCCAAGTT TAACTGTGCG
    TCCGAGCAGG TGACGTTCAA AGCTGGCGGC AGGAGGACGC GATTTCTGAG TACGCCCTGC
    TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGCCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACCAACG CCGTTGGGGG AGCCGTGAGC AGCCAGGCCG CCCCGGCTCC ACCTCCTGCC
    GTGACGTCCA GCAGGAAGGG CATGTTCACG GACGACCTGC ACAAGCTGGT GGACAACTGG
    GCCCGGGACG CCATGAGTCT CTCAGGCAGG CGGGGAAGCA AAGGACACGT GAGCTATGAG
    GGCCCTGGCA TGGCAAGGAA GTTCTCTGCG CCCGGTCAGC TGTGCGTCTC CATGACCTCA
    AACCTGGGCG GCTCTGCCCC CGGCTCTGCA GCGTCAGCTA CCTCTCTAGG GCACTTCCCC
    AAGTCCATGT GCCCCCCTCA GCAGTACGGT TTCCCTACCC CCCCGTTTGG CACGCAGTGG
    AGCGGGACGG GTGGCCCGGC ACCCCAGCCG CTTAGCCAGT TCCAGCCTGT GGGAACAGCC
    TCCTTACAGA ATTTCAACAT CAGCAACTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848478.1
    CDS133..7410
    Translation

    Target ORF information:

    RefSeq Version XM_024992710.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGCT GTGCCAAGTT TAACTGTGCG
    TCCGAGCAGG TGACGTTCAA AGCTGGCGGC AGGAGGACGC GATTTCTGAG TACGCCCTGC
    TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGCCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACCAACG CCGTTGGGGG AGCCGTGAGC AGCCAGGCCG CCCCGGCTCC ACCTCCTGCC
    GTGACGTCCA GCAGGAAGGG CATGTTCACG GACGACCTGC ACAAGCTGGT GGACAACTGG
    GCCCGGGACG CCATGAGTCT CTCAGGCAGG CGGGGAAGCA AAGGACACGT GAGCTATGAG
    GGCCCTGGCA TGGCAAGGAA GTTCTCTGCG CCCGGTCAGC TGTGCGTCTC CATGACCTCA
    AACCTGGGCG GCTCTGCCCC CGGCTCTGCA GCGTCAGCTA CCTCTCTAGG GCACTTCCCC
    AAGTCCATGT GCCCCCCTCA GCAGTACGGT TTCCCTACCC CCCCGTTTGG CACGCAGTGG
    AGCGGGACGG GTGGCCCGGC ACCCCAGCCG CTTAGCCAGT TCCAGCCTGT GGGAACAGCC
    TCCTTACAGA ATTTCAACAT CAGCAACTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234911
    Clone ID Related Accession (Same CDS sequence) XM_024992711.1
    Accession Version XM_024992711.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGC CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACCAACGCCG TTGGGGGAGC CGTGAGCAGC CAGGCCGCCC CGGCTCCACC TCCTGCCGTG
    ACGTCCAGCA GGAAGGGCAT GTTCACGGAC GACCTGCACA AGCTGGTGGA CAACTGGGCC
    CGGGACGCCA TGAGTCTCTC AGGCAGGCGG GGAAGCAAAG GACACGTGAG CTATGAGGGC
    CCTGGCATGG CAAGGAAGTT CTCTGCGCCC GGTCAGCTGT GCGTCTCCAT GACCTCAAAC
    CTGGGCGGCT CTGCCCCCGG CTCTGCAGCG TCAGCTACCT CTCTAGGGCA CTTCCCCAAG
    TCCATGTGCC CCCCTCAGCA GTACGGTTTC CCTACCCCCC CGTTTGGCAC GCAGTGGAGC
    GGGACGGGTG GCCCGGCACC CCAGCCGCTT AGCCAGTTCC AGCCTGTGGG AACAGCCTCC
    TTACAGAATT TCAACATCAG CAACTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848479.1
    CDS133..7407
    Translation

    Target ORF information:

    RefSeq Version XM_024992711.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992711.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGC CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACCAACGCCG TTGGGGGAGC CGTGAGCAGC CAGGCCGCCC CGGCTCCACC TCCTGCCGTG
    ACGTCCAGCA GGAAGGGCAT GTTCACGGAC GACCTGCACA AGCTGGTGGA CAACTGGGCC
    CGGGACGCCA TGAGTCTCTC AGGCAGGCGG GGAAGCAAAG GACACGTGAG CTATGAGGGC
    CCTGGCATGG CAAGGAAGTT CTCTGCGCCC GGTCAGCTGT GCGTCTCCAT GACCTCAAAC
    CTGGGCGGCT CTGCCCCCGG CTCTGCAGCG TCAGCTACCT CTCTAGGGCA CTTCCCCAAG
    TCCATGTGCC CCCCTCAGCA GTACGGTTTC CCTACCCCCC CGTTTGGCAC GCAGTGGAGC
    GGGACGGGTG GCCCGGCACC CCAGCCGCTT AGCCAGTTCC AGCCTGTGGG AACAGCCTCC
    TTACAGAATT TCAACATCAG CAACTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234912
    Clone ID Related Accession (Same CDS sequence) XM_024992712.1
    Accession Version XM_024992712.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAAGGAGTG CAGCCAACAG CCCCCTCTCA GCAGGCGGTG
    CAGTATCCGC TCCCTCAGAC ATCGGCCCCC AGCGAGGCCA CGATTGCACA GCCTGTGAGT
    CAGCCTCCAC CTCCACAGGC CTTGCCACAC GTGTCCGCGG GAAAACAGCT TCCAGTTTCC
    CAGCCAGTAC CAACTCTCCA AGGCGAACCT CAGATCCCAG TTGCAACACA GCCCTCAGTC
    GTTCCCGTCC ATGCTGGTGC TCACTTCCTT CCTGTGGGAC AGCCACTCCC ACCCTCTTTA
    CTGCCCCAGT ACCCCGTCTC TCAGCTGCCC CCCATCTCTC AGCTCCCCTC TGCTCCTCCT
    CACGTGTCTG TGGCTCAGCC AGGCTTCCCA CCCCTGCCGG TCACGATGGC AGCTGGCGTT
    AGTCAGCCTC TGCTCACCTT GGCTTCATCT GCTGCGGCGG CGGCCGTCCC CGGGGCACCC
    ACTGTGGTCC CTAGTCAGCT TCCTGCCCTC CTGCAGCCTG TGACCCAGCT GCCGAGTCAG
    GCTCACCCGC AGCTCCTGCA GCCAGCAGTT CAGTCCATGG GGATCCCCGC CAGCCTTGGA
    CAGACTGCCG AGGTGCCACT TCCCCCTGGA GATATTCTCT ACCAGGGCTT CCCACCTCGA
    CTGCCATCGC AGTACCCAGG AGACTCAAAC ATTGCCCCCT CCTCCAGCGC AGCTTCTGTG
    TGCCTGCCTT CTGCAGTCCT GTCCCCTCCC ATGCCGACAG AGGCGCTGGC TGCCCCGGGT
    TACTTTCCTG CAATGGTGCA GCCCTATGTG GAATCCAGTC TCTTGGTTCC TGTGGGCAGT
    GTAGGAGGAC AGGTCCAGGT GTCCCAGCCG GCAGTGAGCT TAGCACAAGC CCCCACCACA
    TCCTCCCAGC AAGCAGCTCT GGAGAGCACT CAGGTCGTCT CTCAGGTGGC TCCTCCAGAG
    CCAGCTCCCG GGGCTCAGCC ACAGCCTGCC CAGCCAGCCG CGGTGGTCTC TTCTATAGAC
    AGCGCACATT CAGATGTGGC TTCAGGCCTG AGTGATGGCA ACGAGAATGT CCCCTCATCC
    AGCGGGAGGC ACGAGGGCAG GACCACGAAG CGGCTGCATC GGAAGTCTAT AAGGAGCCGC
    TCTCGTCACG AGAAGCCTTC CCGCCCCAAA CTGAGAATTC TCAATGTTTC AAATAAGGGA
    GATCGGGTGG TAGAATGTCA GTTAGAGACT CACAATCGGA AGATGGTTAC ATTCAAATTT
    GATTTAGATG GTGACAACCC TGAGGAGATA GCCACAATTA TGGTGAACAA TGACTTTATT
    CTGGCACTGG AGAGAGATGC GTTTGTGGAG CAAGTGCGGG AGATCATTGA GAAAGCTGAC
    GAGATGCTCA GTGAGGATGT CAGCGTGGAG CCAGAGGGTG ACCAGAGCTT GGAGAGTCTC
    CATGGCAAGG ATGACTATGG CTTCTCAGGC ACCCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CATCTTCCTT GCCACAACAG ATAGCAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGC CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACCAACGCCG TTGGGGGAGC CGTGAGCAGC CAGGCCGCCC CGGCTCCACC TCCTGCCGTG
    ACGTCCAGCA GGAAGGGCAT GTTCACGGAC GACCTGCACA AGCTGGTGGA CAACTGGGCC
    CGGGACGCCA TGAGTCTCTC AGGCAGGCGG GGAAGCAAAG GACACGTGAG CTATGAGGGC
    CCTGGCATGG CAAGGAAGTT CTCTGCGCCC GGTCAGCTGT GCGTCTCCAT GACCTCAAAC
    CTGGGCGGCT CTGCCCCCGG CTCTGCAGCG TCAGCTACCT CTCTAGGGCA CTTCCCCAAG
    TCCATGTGCC CCCCTCAGCA GTACGGTTTC CCTACCCCCC CGTTTGGCAC GCAGTGGAGC
    GGGACGGGTG GCCCGGCACC CCAGCCGCTT AGCCAGTTCC AGCCTGTGGG AACAGCCTCC
    TTACAGAATT TCAACATCAG CAACTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848480.1
    CDS134..7408
    Translation

    Target ORF information:

    RefSeq Version XM_024992712.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992712.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAAGGAGTG CAGCCAACAG CCCCCTCTCA GCAGGCGGTG
    CAGTATCCGC TCCCTCAGAC ATCGGCCCCC AGCGAGGCCA CGATTGCACA GCCTGTGAGT
    CAGCCTCCAC CTCCACAGGC CTTGCCACAC GTGTCCGCGG GAAAACAGCT TCCAGTTTCC
    CAGCCAGTAC CAACTCTCCA AGGCGAACCT CAGATCCCAG TTGCAACACA GCCCTCAGTC
    GTTCCCGTCC ATGCTGGTGC TCACTTCCTT CCTGTGGGAC AGCCACTCCC ACCCTCTTTA
    CTGCCCCAGT ACCCCGTCTC TCAGCTGCCC CCCATCTCTC AGCTCCCCTC TGCTCCTCCT
    CACGTGTCTG TGGCTCAGCC AGGCTTCCCA CCCCTGCCGG TCACGATGGC AGCTGGCGTT
    AGTCAGCCTC TGCTCACCTT GGCTTCATCT GCTGCGGCGG CGGCCGTCCC CGGGGCACCC
    ACTGTGGTCC CTAGTCAGCT TCCTGCCCTC CTGCAGCCTG TGACCCAGCT GCCGAGTCAG
    GCTCACCCGC AGCTCCTGCA GCCAGCAGTT CAGTCCATGG GGATCCCCGC CAGCCTTGGA
    CAGACTGCCG AGGTGCCACT TCCCCCTGGA GATATTCTCT ACCAGGGCTT CCCACCTCGA
    CTGCCATCGC AGTACCCAGG AGACTCAAAC ATTGCCCCCT CCTCCAGCGC AGCTTCTGTG
    TGCCTGCCTT CTGCAGTCCT GTCCCCTCCC ATGCCGACAG AGGCGCTGGC TGCCCCGGGT
    TACTTTCCTG CAATGGTGCA GCCCTATGTG GAATCCAGTC TCTTGGTTCC TGTGGGCAGT
    GTAGGAGGAC AGGTCCAGGT GTCCCAGCCG GCAGTGAGCT TAGCACAAGC CCCCACCACA
    TCCTCCCAGC AAGCAGCTCT GGAGAGCACT CAGGTCGTCT CTCAGGTGGC TCCTCCAGAG
    CCAGCTCCCG GGGCTCAGCC ACAGCCTGCC CAGCCAGCCG CGGTGGTCTC TTCTATAGAC
    AGCGCACATT CAGATGTGGC TTCAGGCCTG AGTGATGGCA ACGAGAATGT CCCCTCATCC
    AGCGGGAGGC ACGAGGGCAG GACCACGAAG CGGCTGCATC GGAAGTCTAT AAGGAGCCGC
    TCTCGTCACG AGAAGCCTTC CCGCCCCAAA CTGAGAATTC TCAATGTTTC AAATAAGGGA
    GATCGGGTGG TAGAATGTCA GTTAGAGACT CACAATCGGA AGATGGTTAC ATTCAAATTT
    GATTTAGATG GTGACAACCC TGAGGAGATA GCCACAATTA TGGTGAACAA TGACTTTATT
    CTGGCACTGG AGAGAGATGC GTTTGTGGAG CAAGTGCGGG AGATCATTGA GAAAGCTGAC
    GAGATGCTCA GTGAGGATGT CAGCGTGGAG CCAGAGGGTG ACCAGAGCTT GGAGAGTCTC
    CATGGCAAGG ATGACTATGG CTTCTCAGGC ACCCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CATCTTCCTT GCCACAACAG ATAGCAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGC CCTTGCAACC AGCTCTGTAG GACCAGCAGC
    ACCAACGCCG TTGGGGGAGC CGTGAGCAGC CAGGCCGCCC CGGCTCCACC TCCTGCCGTG
    ACGTCCAGCA GGAAGGGCAT GTTCACGGAC GACCTGCACA AGCTGGTGGA CAACTGGGCC
    CGGGACGCCA TGAGTCTCTC AGGCAGGCGG GGAAGCAAAG GACACGTGAG CTATGAGGGC
    CCTGGCATGG CAAGGAAGTT CTCTGCGCCC GGTCAGCTGT GCGTCTCCAT GACCTCAAAC
    CTGGGCGGCT CTGCCCCCGG CTCTGCAGCG TCAGCTACCT CTCTAGGGCA CTTCCCCAAG
    TCCATGTGCC CCCCTCAGCA GTACGGTTTC CCTACCCCCC CGTTTGGCAC GCAGTGGAGC
    GGGACGGGTG GCCCGGCACC CCAGCCGCTT AGCCAGTTCC AGCCTGTGGG AACAGCCTCC
    TTACAGAATT TCAACATCAG CAACTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234913
    Clone ID Related Accession (Same CDS sequence) XM_024992713.1
    Accession Version XM_024992713.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGCT GTGCCAAGTT TAACTGTGCG
    TCCGAGCAGG TGACGTTCAA AGCTGGCGGC AGGAGGACGC GATTTCTGAG GAAGATGGTG
    AAAAAAGTCT GCCCTTGCAA CCAGCTCTGT AGGACCAGCA GCACCAACGC CGTTGGGGGA
    GCCGTGAGCA GCCAGGCCGC CCCGGCTCCA CCTCCTGCCG TGACGTCCAG CAGGAAGGGC
    ATGTTCACGG ACGACCTGCA CAAGCTGGTG GACAACTGGG CCCGGGACGC CATGAGTCTC
    TCAGGCAGGC GGGGAAGCAA AGGACACGTG AGCTATGAGG GCCCTGGCAT GGCAAGGAAG
    TTCTCTGCGC CCGGTCAGCT GTGCGTCTCC ATGACCTCAA ACCTGGGCGG CTCTGCCCCC
    GGCTCTGCAG CGTCAGCTAC CTCTCTAGGG CACTTCCCCA AGTCCATGTG CCCCCCTCAG
    CAGTACGGTT TCCCTACCCC CCCGTTTGGC ACGCAGTGGA GCGGGACGGG TGGCCCGGCA
    CCCCAGCCGC TTAGCCAGTT CCAGCCTGTG GGAACAGCCT CCTTACAGAA TTTCAACATC
    AGCAACTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848481.1
    CDS133..7389
    Translation

    Target ORF information:

    RefSeq Version XM_024992713.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992713.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGCT GTGCCAAGTT TAACTGTGCG
    TCCGAGCAGG TGACGTTCAA AGCTGGCGGC AGGAGGACGC GATTTCTGAG GAAGATGGTG
    AAAAAAGTCT GCCCTTGCAA CCAGCTCTGT AGGACCAGCA GCACCAACGC CGTTGGGGGA
    GCCGTGAGCA GCCAGGCCGC CCCGGCTCCA CCTCCTGCCG TGACGTCCAG CAGGAAGGGC
    ATGTTCACGG ACGACCTGCA CAAGCTGGTG GACAACTGGG CCCGGGACGC CATGAGTCTC
    TCAGGCAGGC GGGGAAGCAA AGGACACGTG AGCTATGAGG GCCCTGGCAT GGCAAGGAAG
    TTCTCTGCGC CCGGTCAGCT GTGCGTCTCC ATGACCTCAA ACCTGGGCGG CTCTGCCCCC
    GGCTCTGCAG CGTCAGCTAC CTCTCTAGGG CACTTCCCCA AGTCCATGTG CCCCCCTCAG
    CAGTACGGTT TCCCTACCCC CCCGTTTGGC ACGCAGTGGA GCGGGACGGG TGGCCCGGCA
    CCCCAGCCGC TTAGCCAGTT CCAGCCTGTG GGAACAGCCT CCTTACAGAA TTTCAACATC
    AGCAACTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234914
    Clone ID Related Accession (Same CDS sequence) XM_024992714.1
    Accession Version XM_024992714.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGGAA GATGGTGAAA
    AAAGTCTGCC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CCAACGCCGT TGGGGGAGCC
    GTGAGCAGCC AGGCCGCCCC GGCTCCACCT CCTGCCGTGA CGTCCAGCAG GAAGGGCATG
    TTCACGGACG ACCTGCACAA GCTGGTGGAC AACTGGGCCC GGGACGCCAT GAGTCTCTCA
    GGCAGGCGGG GAAGCAAAGG ACACGTGAGC TATGAGGGCC CTGGCATGGC AAGGAAGTTC
    TCTGCGCCCG GTCAGCTGTG CGTCTCCATG ACCTCAAACC TGGGCGGCTC TGCCCCCGGC
    TCTGCAGCGT CAGCTACCTC TCTAGGGCAC TTCCCCAAGT CCATGTGCCC CCCTCAGCAG
    TACGGTTTCC CTACCCCCCC GTTTGGCACG CAGTGGAGCG GGACGGGTGG CCCGGCACCC
    CAGCCGCTTA GCCAGTTCCA GCCTGTGGGA ACAGCCTCCT TACAGAATTT CAACATCAGC
    AACTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848482.1
    CDS133..7386
    Translation

    Target ORF information:

    RefSeq Version XM_024992714.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992714.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGGAA GATGGTGAAA
    AAAGTCTGCC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CCAACGCCGT TGGGGGAGCC
    GTGAGCAGCC AGGCCGCCCC GGCTCCACCT CCTGCCGTGA CGTCCAGCAG GAAGGGCATG
    TTCACGGACG ACCTGCACAA GCTGGTGGAC AACTGGGCCC GGGACGCCAT GAGTCTCTCA
    GGCAGGCGGG GAAGCAAAGG ACACGTGAGC TATGAGGGCC CTGGCATGGC AAGGAAGTTC
    TCTGCGCCCG GTCAGCTGTG CGTCTCCATG ACCTCAAACC TGGGCGGCTC TGCCCCCGGC
    TCTGCAGCGT CAGCTACCTC TCTAGGGCAC TTCCCCAAGT CCATGTGCCC CCCTCAGCAG
    TACGGTTTCC CTACCCCCCC GTTTGGCACG CAGTGGAGCG GGACGGGTGG CCCGGCACCC
    CAGCCGCTTA GCCAGTTCCA GCCTGTGGGA ACAGCCTCCT TACAGAATTT CAACATCAGC
    AACTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234915
    Clone ID Related Accession (Same CDS sequence) XM_024992715.1
    Accession Version XM_024992715.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGCT GTGCCAAGTT TAACTGTGCG
    TCCGAGCAGG TGACGTTCAA AGCTGGCGGC AGGAGGACGC GATTTCTGAG GACCAGCAGC
    ACCAACGCCG TTGGGGGAGC CGTGAGCAGC CAGGCCGCCC CGGCTCCACC TCCTGCCGTG
    ACGTCCAGCA GGAAGGGCAT GTTCACGGAC GACCTGCACA AGCTGGTGGA CAACTGGGCC
    CGGGACGCCA TGAGTCTCTC AGGCAGGCGG GGAAGCAAAG GACACGTGAG CTATGAGGGC
    CCTGGCATGG CAAGGAAGTT CTCTGCGCCC GGTCAGCTGT GCGTCTCCAT GACCTCAAAC
    CTGGGCGGCT CTGCCCCCGG CTCTGCAGCG TCAGCTACCT CTCTAGGGCA CTTCCCCAAG
    TCCATGTGCC CCCCTCAGCA GTACGGTTTC CCTACCCCCC CGTTTGGCAC GCAGTGGAGC
    GGGACGGGTG GCCCGGCACC CCAGCCGCTT AGCCAGTTCC AGCCTGTGGG AACAGCCTCC
    TTACAGAATT TCAACATCAG CAACTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848483.1
    CDS133..7347
    Translation

    Target ORF information:

    RefSeq Version XM_024992715.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992715.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGCT GTGCCAAGTT TAACTGTGCG
    TCCGAGCAGG TGACGTTCAA AGCTGGCGGC AGGAGGACGC GATTTCTGAG GACCAGCAGC
    ACCAACGCCG TTGGGGGAGC CGTGAGCAGC CAGGCCGCCC CGGCTCCACC TCCTGCCGTG
    ACGTCCAGCA GGAAGGGCAT GTTCACGGAC GACCTGCACA AGCTGGTGGA CAACTGGGCC
    CGGGACGCCA TGAGTCTCTC AGGCAGGCGG GGAAGCAAAG GACACGTGAG CTATGAGGGC
    CCTGGCATGG CAAGGAAGTT CTCTGCGCCC GGTCAGCTGT GCGTCTCCAT GACCTCAAAC
    CTGGGCGGCT CTGCCCCCGG CTCTGCAGCG TCAGCTACCT CTCTAGGGCA CTTCCCCAAG
    TCCATGTGCC CCCCTCAGCA GTACGGTTTC CCTACCCCCC CGTTTGGCAC GCAGTGGAGC
    GGGACGGGTG GCCCGGCACC CCAGCCGCTT AGCCAGTTCC AGCCTGTGGG AACAGCCTCC
    TTACAGAATT TCAACATCAG CAACTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC
    CTGCGGACCA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234916
    Clone ID Related Accession (Same CDS sequence) XM_024992717.1
    Accession Version XM_024992717.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCAG
    CAAGGAGTGC AGCCAACAGC CCCCTCTCAG CAGGCGGTGC AGTATCCGCT CCCTCAGACA
    TCGGCCCCCA GCGAGGCCAC GATTGCACAG CCTGTGAGTC AGCCTCCACC TCCACAGGCC
    TTGCCACACG TGTCCGCGGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTCTCCAA
    GGCGAACCTC AGATCCCAGT TGCAACACAG CCCTCAGTCG TTCCCGTCCA TGCTGGTGCT
    CACTTCCTTC CTGTGGGACA GCCACTCCCA CCCTCTTTAC TGCCCCAGTA CCCCGTCTCT
    CAGCTGCCCC CCATCTCTCA GCTCCCCTCT GCTCCTCCTC ACGTGTCTGT GGCTCAGCCA
    GGCTTCCCAC CCCTGCCGGT CACGATGGCA GCTGGCGTTA GTCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTGCGGCGGC GGCCGTCCCC GGGGCACCCA CTGTGGTCCC TAGTCAGCTT
    CCTGCCCTCC TGCAGCCTGT GACCCAGCTG CCGAGTCAGG CTCACCCGCA GCTCCTGCAG
    CCAGCAGTTC AGTCCATGGG GATCCCCGCC AGCCTTGGAC AGACTGCCGA GGTGCCACTT
    CCCCCTGGAG ATATTCTCTA CCAGGGCTTC CCACCTCGAC TGCCATCGCA GTACCCAGGA
    GACTCAAACA TTGCCCCCTC CTCCAGCGCA GCTTCTGTGT GCCTGCCTTC TGCAGTCCTG
    TCCCCTCCCA TGCCGACAGA GGCGCTGGCT GCCCCGGGTT ACTTTCCTGC AATGGTGCAG
    CCCTATGTGG AATCCAGTCT CTTGGTTCCT GTGGGCAGTG TAGGAGGACA GGTCCAGGTG
    TCCCAGCCGG CAGTGAGCTT AGCACAAGCC CCCACCACAT CCTCCCAGCA AGCAGCTCTG
    GAGAGCACTC AGGTCGTCTC TCAGGTGGCT CCTCCAGAGC CAGCTCCCGG GGCTCAGCCA
    CAGCCTGCCC AGCCAGCCGC GGTGGTCTCT TCTATAGACA GCGCACATTC AGATGTGGCT
    TCAGGCCTGA GTGATGGCAA CGAGAATGTC CCCTCATCCA GCGGGAGGCA CGAGGGCAGG
    ACCACGAAGC GGCTGCATCG GAAGTCTATA AGGAGCCGCT CTCGTCACGA GAAGCCTTCC
    CGCCCCAAAC TGAGAATTCT CAATGTTTCA AATAAGGGAG ATCGGGTGGT AGAATGTCAG
    TTAGAGACTC ACAATCGGAA GATGGTTACA TTCAAATTTG ATTTAGATGG TGACAACCCT
    GAGGAGATAG CCACAATTAT GGTGAACAAT GACTTTATTC TGGCACTGGA GAGAGATGCG
    TTTGTGGAGC AAGTGCGGGA GATCATTGAG AAAGCTGACG AGATGCTCAG TGAGGATGTC
    AGCGTGGAGC CAGAGGGTGA CCAGAGCTTG GAGAGTCTCC ATGGCAAGGA TGACTATGGC
    TTCTCAGGCA CCCAGAAATT GGAAGGAGAG TTCAAACAAC CAATTCCTGC ATCTTCCTTG
    CCACAACAGA TAGCAGGCAT TCCTGCCAGT TCTTTAACTC AAGTTGTTCA TTCTGCTGGA
    AGACGGTTTA TAGTAAGTCC TGTGCCAGAA AGTCGATTAC GAGAATCAAA ACTTTTCACT
    GAAATTTCAG ATACGGTTGC TGCTTCCACA TCTGCTGGCC CTGGAATGAA CTTGTCTCAC
    TCTGCCTCAT CCCTTAGTCT GCAGCAGGCC TTTTCTGAAC TTAGACATGC TCGGATGGCA
    GAAGGGCCGA GTACGGCCCC TCCCCACTTT GGTCAGACAG GCCCCGTGTT TCCAGCAGTG
    CCTCCCACCC TGAGCAGTGC CCCTGGAGCC CCAGCCGCAG CAGCCGCAGC CTCGGTGTCA
    GCCCCTGCCC CAAGCCACCC TCTTAGTGAC GTGACCACAT CGGTCATTCA GACTGAGGTG
    ACAGTGGCCC CTGGAACAGG TGTCATGCCT CCCAGTGGTC TCCCTGTACC CCCTGTGTCT
    GAGTCCCTGG CACCTTCCAG CGTGGCCCCC AGCGTCACCA TCCCTGCAGT TCTCTCGGCA
    CTACCAGCGC CCCAGGCGGC ACAGGCTCCC ACCCCTGGCA GCGGGGCCTC CAGTGCAGGC
    ACTTTCCCCT CTGCACCCGC ATCCCTGACA CCAGCCTCTG CTGTGGGCAG CGCTGTTGCC
    TCTGCTGCTC AGCTTCCGCC TGTGCCGTCT CAGCCCGCGG TAGGCAGCTC GACCGGGGTG
    GCCACGCTGA CAGCCGTGCC CGCTGCCGCT CTGGGCCCTA GCCTCACGGC ACTGCCGTCC
    CTTGCTCTCA GCAGCAGTAC CTCTGCCCCA GCTCTGGCAG AGACAGTGCT GGTCAGTGCC
    CACGCACTGG ATAAGACGGC CCATAGTAGC ACCACTGGCC TGGCTCTGTC CCTTCCTGCC
    ACCTCGTCCT CTCCCGCCCC TGGAGCAGGA GTGTCCAGTG CTGTTTCTCA GCCTACTGCT
    GCGGCTCACC CTTTGGTCCT CCCATCAGTC GTAACCTCTA CTCCCGTACT CTCCCAGGCA
    GGACCCATGT CAGCACCTCT ACTGCCCCAG GTACCCGGTA TTCCACCCCT GGTACAACCT
    GTTGTCAGCG TGCCTGCTGT GCAACAGACC CTGGTTCATA GTCAGCCTCA GCCCGCTCCA
    CTCCCCAACC AGCCCCACAC TCACTGTCCC GAGATAGACG CCGACACTCA ACCCAAAGCT
    CCCGGGATCG ACGACATCAA GACTTTGGAG GAAAAGCTGC GATCTCTCTT TAGTGAGCAC
    AGTTCTTCTG GAGCCCAACA CGCCCCCGTC TCTCTGGAGA CCTCGCTCAC TGTAGAGACC
    GCTGTCACAG CAGGCCTCCC AACCACTGCT GTTGCACCAA GCAAGCCTCT GACTTCCACT
    GCCAGTACTT GCTTGCCACC AACCAGTCTG CCCTTAGGAG CAGCTGGCTT GTCAGTTATA
    CCAATGGTCA CTCCTGGTCA AGTTTCCACC CCAGTCAGCT CTGCGCCAGG AGTGAAACTT
    GAAACTGCTC CAGCAAAGCC ACCTTTAACT AAGCCTCCAG TGCTGCCCAT GGGCACTGAA
    CTTCCAGCTG GTACTCCACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCACTAACT
    CAGCCCCCGC AGCCCCTGGG AGACCTGGAC GCTCAGCTGA GAAGAACCCT CAGTCCCGAG
    AGTGGCATGC TGACGTCTGC CGTTGGACCC GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG
    GAGACCGAAG CCCAACTTCC AAAGGATGTT CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT
    GTCCTTGCGC CCACTTCCAC AGCTGGTGTT CTGAAGATGG GGCGATTTCA GGTCTCAGTT
    GCAATGGATG ACCCCCAGAA GGAGGGTAAA AGTAAGTCGG AGGATGCCAA GTCTGTCCAC
    TTCGCATCCA GCACGTCAGA GTCCTCGGTG CTGTCAAGCA GCAGCCCCGA GAGCACCGTG
    GTGAAAGCAG AGCCCAACGG GCTGGCCGTC CGGGGCGCCT CTGCAGACAT GCCTGACAGC
    GCCCACCAGC CACCTGCCTC AGAAGCTGGG CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG
    ACCACCACAG CGAGCAGAGT GGGTCGCTTC TCTGTGTCCA GAACTGAAGA CAAGATGGCT
    GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT CCTTTCATGG ACTCAGAACA CGACATTCCT
    CCCACCGTGA CGCCAAAGAA AGAGAAACCC GAACTGTCTG AGCCTTCGCA TCTGAACGGG
    CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC AGCCGGGACG TGGATGCTGG CTCAGGGAGC
    CCTCACTCCC CTCCGCAGCT GAGCTCCAAG AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC
    CTCAGCAATT CCTTCAACTC TTCCTACATG AGCAGCGACA ATGAGTCAGA CATTGAAGAC
    GAGGACTTAA AGTTAGAGCT TCGCCGACTA CGGGAAAAAC ATCTTAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA CGAAATTGAG TCTTTATACA CCAAACTGGG CAAGGTTCCT
    CCTGCTGTCA TCATTCCCCC AGCAGCCCCC CTTGCAGGGA GAAGGAGGCG ACCCACCAAA
    AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC TCTTTGGGGA ATAAAAGCCC TGGACCAGGT
    ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC TTGCACCCTC AGCAGACCCT CCCCGCTCCT
    GGCAGCGTCC CGGAGACAGG GCCCAACCAG CTGTTACAGC CTGTGAAGCC ATCTCCTTCC
    AGTGACAACC TGTACTCGGC CTTTACCAGC GATGGTGCCA TTTCAGTACC AAGCCTTTCT
    GCGCCAGGTC AAGGCTGTGC CAAGTTTAAC TGTGCGTCCG AGCAGGTGAC GTTCAAAGCT
    GGCGGCAGGA GGACGCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA
    AAAGTCTGCC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CCAACGCCGT TGGGGGAGCC
    GTGAGCAGCC AGGCCGCCCC GGCTCCACCT CCTGCCGTGA CGTCCAGCAG GAAGGGCATG
    TTCACGGACG ACCTGCACAA GCTGGTGGAC AACTGGGCCC GGGACGCCAT GAGTCTCTCA
    GGCAGGCGGG GAAGCAAAGG ACACGTGAGC TATGAGGGCC CTGGCATGGC AAGGAAGTTC
    TCTGCGCCCG GTCAGCTGTG CGTCTCCATG ACCTCAAACC TGGGCGGCTC TGCCCCCGGC
    TCTGCAGCGT CAGCTACCTC TCTAGGGCAC TTCCCCAAGT CCATGTGCCC CCCTCAGCAG
    TACGGTTTCC CTACCCCCCC GTTTGGCACG CAGTGGAGCG GGACGGGTGG CCCGGCACCC
    CAGCCGCTTA GCCAGTTCCA GCCTGTGGGA ACAGCCTCCT TACAGAATTT CAACATCAGC
    AACTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848485.1
    CDS133..7326
    Translation

    Target ORF information:

    RefSeq Version XM_024992717.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992717.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCAG
    CAAGGAGTGC AGCCAACAGC CCCCTCTCAG CAGGCGGTGC AGTATCCGCT CCCTCAGACA
    TCGGCCCCCA GCGAGGCCAC GATTGCACAG CCTGTGAGTC AGCCTCCACC TCCACAGGCC
    TTGCCACACG TGTCCGCGGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTCTCCAA
    GGCGAACCTC AGATCCCAGT TGCAACACAG CCCTCAGTCG TTCCCGTCCA TGCTGGTGCT
    CACTTCCTTC CTGTGGGACA GCCACTCCCA CCCTCTTTAC TGCCCCAGTA CCCCGTCTCT
    CAGCTGCCCC CCATCTCTCA GCTCCCCTCT GCTCCTCCTC ACGTGTCTGT GGCTCAGCCA
    GGCTTCCCAC CCCTGCCGGT CACGATGGCA GCTGGCGTTA GTCAGCCTCT GCTCACCTTG
    GCTTCATCTG CTGCGGCGGC GGCCGTCCCC GGGGCACCCA CTGTGGTCCC TAGTCAGCTT
    CCTGCCCTCC TGCAGCCTGT GACCCAGCTG CCGAGTCAGG CTCACCCGCA GCTCCTGCAG
    CCAGCAGTTC AGTCCATGGG GATCCCCGCC AGCCTTGGAC AGACTGCCGA GGTGCCACTT
    CCCCCTGGAG ATATTCTCTA CCAGGGCTTC CCACCTCGAC TGCCATCGCA GTACCCAGGA
    GACTCAAACA TTGCCCCCTC CTCCAGCGCA GCTTCTGTGT GCCTGCCTTC TGCAGTCCTG
    TCCCCTCCCA TGCCGACAGA GGCGCTGGCT GCCCCGGGTT ACTTTCCTGC AATGGTGCAG
    CCCTATGTGG AATCCAGTCT CTTGGTTCCT GTGGGCAGTG TAGGAGGACA GGTCCAGGTG
    TCCCAGCCGG CAGTGAGCTT AGCACAAGCC CCCACCACAT CCTCCCAGCA AGCAGCTCTG
    GAGAGCACTC AGGTCGTCTC TCAGGTGGCT CCTCCAGAGC CAGCTCCCGG GGCTCAGCCA
    CAGCCTGCCC AGCCAGCCGC GGTGGTCTCT TCTATAGACA GCGCACATTC AGATGTGGCT
    TCAGGCCTGA GTGATGGCAA CGAGAATGTC CCCTCATCCA GCGGGAGGCA CGAGGGCAGG
    ACCACGAAGC GGCTGCATCG GAAGTCTATA AGGAGCCGCT CTCGTCACGA GAAGCCTTCC
    CGCCCCAAAC TGAGAATTCT CAATGTTTCA AATAAGGGAG ATCGGGTGGT AGAATGTCAG
    TTAGAGACTC ACAATCGGAA GATGGTTACA TTCAAATTTG ATTTAGATGG TGACAACCCT
    GAGGAGATAG CCACAATTAT GGTGAACAAT GACTTTATTC TGGCACTGGA GAGAGATGCG
    TTTGTGGAGC AAGTGCGGGA GATCATTGAG AAAGCTGACG AGATGCTCAG TGAGGATGTC
    AGCGTGGAGC CAGAGGGTGA CCAGAGCTTG GAGAGTCTCC ATGGCAAGGA TGACTATGGC
    TTCTCAGGCA CCCAGAAATT GGAAGGAGAG TTCAAACAAC CAATTCCTGC ATCTTCCTTG
    CCACAACAGA TAGCAGGCAT TCCTGCCAGT TCTTTAACTC AAGTTGTTCA TTCTGCTGGA
    AGACGGTTTA TAGTAAGTCC TGTGCCAGAA AGTCGATTAC GAGAATCAAA ACTTTTCACT
    GAAATTTCAG ATACGGTTGC TGCTTCCACA TCTGCTGGCC CTGGAATGAA CTTGTCTCAC
    TCTGCCTCAT CCCTTAGTCT GCAGCAGGCC TTTTCTGAAC TTAGACATGC TCGGATGGCA
    GAAGGGCCGA GTACGGCCCC TCCCCACTTT GGTCAGACAG GCCCCGTGTT TCCAGCAGTG
    CCTCCCACCC TGAGCAGTGC CCCTGGAGCC CCAGCCGCAG CAGCCGCAGC CTCGGTGTCA
    GCCCCTGCCC CAAGCCACCC TCTTAGTGAC GTGACCACAT CGGTCATTCA GACTGAGGTG
    ACAGTGGCCC CTGGAACAGG TGTCATGCCT CCCAGTGGTC TCCCTGTACC CCCTGTGTCT
    GAGTCCCTGG CACCTTCCAG CGTGGCCCCC AGCGTCACCA TCCCTGCAGT TCTCTCGGCA
    CTACCAGCGC CCCAGGCGGC ACAGGCTCCC ACCCCTGGCA GCGGGGCCTC CAGTGCAGGC
    ACTTTCCCCT CTGCACCCGC ATCCCTGACA CCAGCCTCTG CTGTGGGCAG CGCTGTTGCC
    TCTGCTGCTC AGCTTCCGCC TGTGCCGTCT CAGCCCGCGG TAGGCAGCTC GACCGGGGTG
    GCCACGCTGA CAGCCGTGCC CGCTGCCGCT CTGGGCCCTA GCCTCACGGC ACTGCCGTCC
    CTTGCTCTCA GCAGCAGTAC CTCTGCCCCA GCTCTGGCAG AGACAGTGCT GGTCAGTGCC
    CACGCACTGG ATAAGACGGC CCATAGTAGC ACCACTGGCC TGGCTCTGTC CCTTCCTGCC
    ACCTCGTCCT CTCCCGCCCC TGGAGCAGGA GTGTCCAGTG CTGTTTCTCA GCCTACTGCT
    GCGGCTCACC CTTTGGTCCT CCCATCAGTC GTAACCTCTA CTCCCGTACT CTCCCAGGCA
    GGACCCATGT CAGCACCTCT ACTGCCCCAG GTACCCGGTA TTCCACCCCT GGTACAACCT
    GTTGTCAGCG TGCCTGCTGT GCAACAGACC CTGGTTCATA GTCAGCCTCA GCCCGCTCCA
    CTCCCCAACC AGCCCCACAC TCACTGTCCC GAGATAGACG CCGACACTCA ACCCAAAGCT
    CCCGGGATCG ACGACATCAA GACTTTGGAG GAAAAGCTGC GATCTCTCTT TAGTGAGCAC
    AGTTCTTCTG GAGCCCAACA CGCCCCCGTC TCTCTGGAGA CCTCGCTCAC TGTAGAGACC
    GCTGTCACAG CAGGCCTCCC AACCACTGCT GTTGCACCAA GCAAGCCTCT GACTTCCACT
    GCCAGTACTT GCTTGCCACC AACCAGTCTG CCCTTAGGAG CAGCTGGCTT GTCAGTTATA
    CCAATGGTCA CTCCTGGTCA AGTTTCCACC CCAGTCAGCT CTGCGCCAGG AGTGAAACTT
    GAAACTGCTC CAGCAAAGCC ACCTTTAACT AAGCCTCCAG TGCTGCCCAT GGGCACTGAA
    CTTCCAGCTG GTACTCCACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCACTAACT
    CAGCCCCCGC AGCCCCTGGG AGACCTGGAC GCTCAGCTGA GAAGAACCCT CAGTCCCGAG
    AGTGGCATGC TGACGTCTGC CGTTGGACCC GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG
    GAGACCGAAG CCCAACTTCC AAAGGATGTT CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT
    GTCCTTGCGC CCACTTCCAC AGCTGGTGTT CTGAAGATGG GGCGATTTCA GGTCTCAGTT
    GCAATGGATG ACCCCCAGAA GGAGGGTAAA AGTAAGTCGG AGGATGCCAA GTCTGTCCAC
    TTCGCATCCA GCACGTCAGA GTCCTCGGTG CTGTCAAGCA GCAGCCCCGA GAGCACCGTG
    GTGAAAGCAG AGCCCAACGG GCTGGCCGTC CGGGGCGCCT CTGCAGACAT GCCTGACAGC
    GCCCACCAGC CACCTGCCTC AGAAGCTGGG CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG
    ACCACCACAG CGAGCAGAGT GGGTCGCTTC TCTGTGTCCA GAACTGAAGA CAAGATGGCT
    GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT CCTTTCATGG ACTCAGAACA CGACATTCCT
    CCCACCGTGA CGCCAAAGAA AGAGAAACCC GAACTGTCTG AGCCTTCGCA TCTGAACGGG
    CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC AGCCGGGACG TGGATGCTGG CTCAGGGAGC
    CCTCACTCCC CTCCGCAGCT GAGCTCCAAG AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC
    CTCAGCAATT CCTTCAACTC TTCCTACATG AGCAGCGACA ATGAGTCAGA CATTGAAGAC
    GAGGACTTAA AGTTAGAGCT TCGCCGACTA CGGGAAAAAC ATCTTAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA CGAAATTGAG TCTTTATACA CCAAACTGGG CAAGGTTCCT
    CCTGCTGTCA TCATTCCCCC AGCAGCCCCC CTTGCAGGGA GAAGGAGGCG ACCCACCAAA
    AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC TCTTTGGGGA ATAAAAGCCC TGGACCAGGT
    ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC TTGCACCCTC AGCAGACCCT CCCCGCTCCT
    GGCAGCGTCC CGGAGACAGG GCCCAACCAG CTGTTACAGC CTGTGAAGCC ATCTCCTTCC
    AGTGACAACC TGTACTCGGC CTTTACCAGC GATGGTGCCA TTTCAGTACC AAGCCTTTCT
    GCGCCAGGTC AAGGCTGTGC CAAGTTTAAC TGTGCGTCCG AGCAGGTGAC GTTCAAAGCT
    GGCGGCAGGA GGACGCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA
    AAAGTCTGCC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CCAACGCCGT TGGGGGAGCC
    GTGAGCAGCC AGGCCGCCCC GGCTCCACCT CCTGCCGTGA CGTCCAGCAG GAAGGGCATG
    TTCACGGACG ACCTGCACAA GCTGGTGGAC AACTGGGCCC GGGACGCCAT GAGTCTCTCA
    GGCAGGCGGG GAAGCAAAGG ACACGTGAGC TATGAGGGCC CTGGCATGGC AAGGAAGTTC
    TCTGCGCCCG GTCAGCTGTG CGTCTCCATG ACCTCAAACC TGGGCGGCTC TGCCCCCGGC
    TCTGCAGCGT CAGCTACCTC TCTAGGGCAC TTCCCCAAGT CCATGTGCCC CCCTCAGCAG
    TACGGTTTCC CTACCCCCCC GTTTGGCACG CAGTGGAGCG GGACGGGTGG CCCGGCACCC
    CAGCCGCTTA GCCAGTTCCA GCCTGTGGGA ACAGCCTCCT TACAGAATTT CAACATCAGC
    AACTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234917
    Clone ID Related Accession (Same CDS sequence) XM_024992718.1
    Accession Version XM_024992718.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCACCCCCGC AGCCCCTGGG AGACCTGGAC GCTCAGCTGA GAAGAACCCT CAGTCCCGAG
    AGTGGCATGC TGACGTCTGC CGTTGGACCC GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG
    GAGACCGAAG CCCAACTTCC AAAGGATGTT CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT
    GTCCTTGCGC CCACTTCCAC AGCTGGTGTT CTGAAGATGG GGCGATTTCA GGTCTCAGTT
    GCAATGGATG ACCCCCAGAA GGAGGGTAAA AGTAAGTCGG AGGATGCCAA GTCTGTCCAC
    TTCGCATCCA GCACGTCAGA GTCCTCGGTG CTGTCAAGCA GCAGCCCCGA GAGCACCGTG
    GTGAAAGCAG AGCCCAACGG GCTGGCCGTC CGGGGCGCCT CTGCAGACAT GCCTGACAGC
    GCCCACCAGC CACCTGCCTC AGAAGCTGGG CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG
    ACCACCACAG CGAGCAGAGT GGGTCGCTTC TCTGTGTCCA GAACTGAAGA CAAGATGGCT
    GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT CCTTTCATGG ACTCAGAACA CGACATTCCT
    CCCACCGTGA CGCCAAAGAA AGAGAAACCC GAACTGTCTG AGCCTTCGCA TCTGAACGGG
    CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC AGCCGGGACG TGGATGCTGG CTCAGGGAGC
    CCTCACTCCC CTCCGCAGCT GAGCTCCAAG AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC
    CTCAGCAATT CCTTCAACTC TTCCTACATG AGCAGCGACA ATGAGTCAGA CATTGAAGAC
    GAGGACTTAA AGTTAGAGCT TCGCCGACTA CGGGAAAAAC ATCTTAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA CGAAATTGAG TCTTTATACA CCAAACTGGG CAAGGTTCCT
    CCTGCTGTCA TCATTCCCCC AGCAGCCCCC CTTGCAGGGA GAAGGAGGCG ACCCACCAAA
    AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC TCTTTGGGGA ATAAAAGCCC TGGACCAGGT
    ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC TTGCACCCTC AGCAGACCCT CCCCGCTCCT
    GGCAGCGTCC CGGAGACAGG GCCCAACCAG CTGTTACAGC CTGTGAAGCC ATCTCCTTCC
    AGTGACAACC TGTACTCGGC CTTTACCAGC GATGGTGCCA TTTCAGTACC AAGCCTTTCT
    GCGCCAGGTC AAGGCTGTGC CAAGTTTAAC TGTGCGTCCG AGCAGGTGAC GTTCAAAGCT
    GGCGGCAGGA GGACGCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA
    AAAGTCTGCC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CCAACGCCGT TGGGGGAGCC
    GTGAGCAGCC AGGCCGCCCC GGCTCCACCT CCTGCCGTGA CGTCCAGCAG GAAGGGCATG
    TTCACGGACG ACCTGCACAA GCTGGTGGAC AACTGGGCCC GGGACGCCAT GAGTCTCTCA
    GGCAGGCGGG GAAGCAAAGG ACACGTGAGC TATGAGGGCC CTGGCATGGC AAGGAAGTTC
    TCTGCGCCCG GTCAGCTGTG CGTCTCCATG ACCTCAAACC TGGGCGGCTC TGCCCCCGGC
    TCTGCAGCGT CAGCTACCTC TCTAGGGCAC TTCCCCAAGT CCATGTGCCC CCCTCAGCAG
    TACGGTTTCC CTACCCCCCC GTTTGGCACG CAGTGGAGCG GGACGGGTGG CCCGGCACCC
    CAGCCGCTTA GCCAGTTCCA GCCTGTGGGA ACAGCCTCCT TACAGAATTT CAACATCAGC
    AACTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848486.1
    CDS134..7327
    Translation

    Target ORF information:

    RefSeq Version XM_024992718.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992718.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCACCCCCGC AGCCCCTGGG AGACCTGGAC GCTCAGCTGA GAAGAACCCT CAGTCCCGAG
    AGTGGCATGC TGACGTCTGC CGTTGGACCC GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG
    GAGACCGAAG CCCAACTTCC AAAGGATGTT CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT
    GTCCTTGCGC CCACTTCCAC AGCTGGTGTT CTGAAGATGG GGCGATTTCA GGTCTCAGTT
    GCAATGGATG ACCCCCAGAA GGAGGGTAAA AGTAAGTCGG AGGATGCCAA GTCTGTCCAC
    TTCGCATCCA GCACGTCAGA GTCCTCGGTG CTGTCAAGCA GCAGCCCCGA GAGCACCGTG
    GTGAAAGCAG AGCCCAACGG GCTGGCCGTC CGGGGCGCCT CTGCAGACAT GCCTGACAGC
    GCCCACCAGC CACCTGCCTC AGAAGCTGGG CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG
    ACCACCACAG CGAGCAGAGT GGGTCGCTTC TCTGTGTCCA GAACTGAAGA CAAGATGGCT
    GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT CCTTTCATGG ACTCAGAACA CGACATTCCT
    CCCACCGTGA CGCCAAAGAA AGAGAAACCC GAACTGTCTG AGCCTTCGCA TCTGAACGGG
    CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC AGCCGGGACG TGGATGCTGG CTCAGGGAGC
    CCTCACTCCC CTCCGCAGCT GAGCTCCAAG AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC
    CTCAGCAATT CCTTCAACTC TTCCTACATG AGCAGCGACA ATGAGTCAGA CATTGAAGAC
    GAGGACTTAA AGTTAGAGCT TCGCCGACTA CGGGAAAAAC ATCTTAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA CGAAATTGAG TCTTTATACA CCAAACTGGG CAAGGTTCCT
    CCTGCTGTCA TCATTCCCCC AGCAGCCCCC CTTGCAGGGA GAAGGAGGCG ACCCACCAAA
    AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC TCTTTGGGGA ATAAAAGCCC TGGACCAGGT
    ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC TTGCACCCTC AGCAGACCCT CCCCGCTCCT
    GGCAGCGTCC CGGAGACAGG GCCCAACCAG CTGTTACAGC CTGTGAAGCC ATCTCCTTCC
    AGTGACAACC TGTACTCGGC CTTTACCAGC GATGGTGCCA TTTCAGTACC AAGCCTTTCT
    GCGCCAGGTC AAGGCTGTGC CAAGTTTAAC TGTGCGTCCG AGCAGGTGAC GTTCAAAGCT
    GGCGGCAGGA GGACGCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA
    AAAGTCTGCC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CCAACGCCGT TGGGGGAGCC
    GTGAGCAGCC AGGCCGCCCC GGCTCCACCT CCTGCCGTGA CGTCCAGCAG GAAGGGCATG
    TTCACGGACG ACCTGCACAA GCTGGTGGAC AACTGGGCCC GGGACGCCAT GAGTCTCTCA
    GGCAGGCGGG GAAGCAAAGG ACACGTGAGC TATGAGGGCC CTGGCATGGC AAGGAAGTTC
    TCTGCGCCCG GTCAGCTGTG CGTCTCCATG ACCTCAAACC TGGGCGGCTC TGCCCCCGGC
    TCTGCAGCGT CAGCTACCTC TCTAGGGCAC TTCCCCAAGT CCATGTGCCC CCCTCAGCAG
    TACGGTTTCC CTACCCCCCC GTTTGGCACG CAGTGGAGCG GGACGGGTGG CCCGGCACCC
    CAGCCGCTTA GCCAGTTCCA GCCTGTGGGA ACAGCCTCCT TACAGAATTT CAACATCAGC
    AACTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234918
    Clone ID Related Accession (Same CDS sequence) XM_024992719.1
    Accession Version XM_024992719.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCAA
    GGAGTGCAGC CAACAGCCCC CTCTCAGCAG GCGGTGCAGT ATCCGCTCCC TCAGACATCG
    GCCCCCAGCG AGGCCACGAT TGCACAGCCT GTGAGTCAGC CTCCACCTCC ACAGGCCTTG
    CCACACGTGT CCGCGGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TCTCCAAGGC
    GAACCTCAGA TCCCAGTTGC AACACAGCCC TCAGTCGTTC CCGTCCATGC TGGTGCTCAC
    TTCCTTCCTG TGGGACAGCC ACTCCCACCC TCTTTACTGC CCCAGTACCC CGTCTCTCAG
    CTGCCCCCCA TCTCTCAGCT CCCCTCTGCT CCTCCTCACG TGTCTGTGGC TCAGCCAGGC
    TTCCCACCCC TGCCGGTCAC GATGGCAGCT GGCGTTAGTC AGCCTCTGCT CACCTTGGCT
    TCATCTGCTG CGGCGGCGGC CGTCCCCGGG GCACCCACTG TGGTCCCTAG TCAGCTTCCT
    GCCCTCCTGC AGCCTGTGAC CCAGCTGCCG AGTCAGGCTC ACCCGCAGCT CCTGCAGCCA
    GCAGTTCAGT CCATGGGGAT CCCCGCCAGC CTTGGACAGA CTGCCGAGGT GCCACTTCCC
    CCTGGAGATA TTCTCTACCA GGGCTTCCCA CCTCGACTGC CATCGCAGTA CCCAGGAGAC
    TCAAACATTG CCCCCTCCTC CAGCGCAGCT TCTGTGTGCC TGCCTTCTGC AGTCCTGTCC
    CCTCCCATGC CGACAGAGGC GCTGGCTGCC CCGGGTTACT TTCCTGCAAT GGTGCAGCCC
    TATGTGGAAT CCAGTCTCTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT CCAGGTGTCC
    CAGCCGGCAG TGAGCTTAGC ACAAGCCCCC ACCACATCCT CCCAGCAAGC AGCTCTGGAG
    AGCACTCAGG TCGTCTCTCA GGTGGCTCCT CCAGAGCCAG CTCCCGGGGC TCAGCCACAG
    CCTGCCCAGC CAGCCGCGGT GGTCTCTTCT ATAGACAGCG CACATTCAGA TGTGGCTTCA
    GGCCTGAGTG ATGGCAACGA GAATGTCCCC TCATCCAGCG GGAGGCACGA GGGCAGGACC
    ACGAAGCGGC TGCATCGGAA GTCTATAAGG AGCCGCTCTC GTCACGAGAA GCCTTCCCGC
    CCCAAACTGA GAATTCTCAA TGTTTCAAAT AAGGGAGATC GGGTGGTAGA ATGTCAGTTA
    GAGACTCACA ATCGGAAGAT GGTTACATTC AAATTTGATT TAGATGGTGA CAACCCTGAG
    GAGATAGCCA CAATTATGGT GAACAATGAC TTTATTCTGG CACTGGAGAG AGATGCGTTT
    GTGGAGCAAG TGCGGGAGAT CATTGAGAAA GCTGACGAGA TGCTCAGTGA GGATGTCAGC
    GTGGAGCCAG AGGGTGACCA GAGCTTGGAG AGTCTCCATG GCAAGGATGA CTATGGCTTC
    TCAGGCACCC AGAAATTGGA AGGAGAGTTC AAACAACCAA TTCCTGCATC TTCCTTGCCA
    CAACAGATAG CAGGCATTCC TGCCAGTTCT TTAACTCAAG TTGTTCATTC TGCTGGAAGA
    CGGTTTATAG TAAGTCCTGT GCCAGAAAGT CGATTACGAG AATCAAAACT TTTCACTGAA
    ATTTCAGATA CGGTTGCTGC TTCCACATCT GCTGGCCCTG GAATGAACTT GTCTCACTCT
    GCCTCATCCC TTAGTCTGCA GCAGGCCTTT TCTGAACTTA GACATGCTCG GATGGCAGAA
    GGGCCGAGTA CGGCCCCTCC CCACTTTGGT CAGACAGGCC CCGTGTTTCC AGCAGTGCCT
    CCCACCCTGA GCAGTGCCCC TGGAGCCCCA GCCGCAGCAG CCGCAGCCTC GGTGTCAGCC
    CCTGCCCCAA GCCACCCTCT TAGTGACGTG ACCACATCGG TCATTCAGAC TGAGGTGACA
    GTGGCCCCTG GAACAGGTGT CATGCCTCCC AGTGGTCTCC CTGTACCCCC TGTGTCTGAG
    TCCCTGGCAC CTTCCAGCGT GGCCCCCAGC GTCACCATCC CTGCAGTTCT CTCGGCACTA
    CCAGCGCCCC AGGCGGCACA GGCTCCCACC CCTGGCAGCG GGGCCTCCAG TGCAGGCACT
    TTCCCCTCTG CACCCGCATC CCTGACACCA GCCTCTGCTG TGGGCAGCGC TGTTGCCTCT
    GCTGCTCAGC TTCCGCCTGT GCCGTCTCAG CCCGCGGTAG GCAGCTCGAC CGGGGTGGCC
    ACGCTGACAG CCGTGCCCGC TGCCGCTCTG GGCCCTAGCC TCACGGCACT GCCGTCCCTT
    GCTCTCAGCA GCAGTACCTC TGCCCCAGCT CTGGCAGAGA CAGTGCTGGT CAGTGCCCAC
    GCACTGGATA AGACGGCCCA TAGTAGCACC ACTGGCCTGG CTCTGTCCCT TCCTGCCACC
    TCGTCCTCTC CCGCCCCTGG AGCAGGAGTG TCCAGTGCTG TTTCTCAGCC TACTGCTGCG
    GCTCACCCTT TGGTCCTCCC ATCAGTCGTA ACCTCTACTC CCGTACTCTC CCAGGCAGGA
    CCCATGTCAG CACCTCTACT GCCCCAGGTA CCCGGTATTC CACCCCTGGT ACAACCTGTT
    GTCAGCGTGC CTGCTGTGCA ACAGACCCTG GTTCATAGTC AGCCTCAGCC CGCTCCACTC
    CCCAACCAGC CCCACACTCA CTGTCCCGAG ATAGACGCCG ACACTCAACC CAAAGCTCCC
    GGGATCGACG ACATCAAGAC TTTGGAGGAA AAGCTGCGAT CTCTCTTTAG TGAGCACAGT
    TCTTCTGGAG CCCAACACGC CCCCGTCTCT CTGGAGACCT CGCTCACTGT AGAGACCGCT
    GTCACAGCAG GCCTCCCAAC CACTGCTGTT GCACCAAGCA AGCCTCTGAC TTCCACTGCC
    AGTACTTGCT TGCCACCAAC CAGTCTGCCC TTAGGAGCAG CTGGCTTGTC AGTTATACCA
    ATGGTCACTC CTGGTCAAGT TTCCACCCCA GTCAGCTCTG CGCCAGGAGT GAAACTTGAA
    ACTGCTCCAG CAAAGCCACC TTTAACTAAG CCTCCAGTGC TGCCCATGGG CACTGAACTT
    CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG
    CCCCCGCAGC CCCTGGGAGA CCTGGACGCT CAGCTGAGAA GAACCCTCAG TCCCGAGAGT
    GGCATGCTGA CGTCTGCCGT TGGACCCGTG TCTGTGGTGG CTCCAACAGC AGTTGTGGAG
    ACCGAAGCCC AACTTCCAAA GGATGTTCTT TCAGTCTCTC AAGTCACAGA AGCTCCTGTC
    CTTGCGCCCA CTTCCACAGC TGGTGTTCTG AAGATGGGGC GATTTCAGGT CTCAGTTGCA
    ATGGATGACC CCCAGAAGGA GGGTAAAAGT AAGTCGGAGG ATGCCAAGTC TGTCCACTTC
    GCATCCAGCA CGTCAGAGTC CTCGGTGCTG TCAAGCAGCA GCCCCGAGAG CACCGTGGTG
    AAAGCAGAGC CCAACGGGCT GGCCGTCCGG GGCGCCTCTG CAGACATGCC TGACAGCGCC
    CACCAGCCAC CTGCCTCAGA AGCTGGGCAG CCTAGCAAGG TGGGCCGTTT TCAGGTGACC
    ACCACAGCGA GCAGAGTGGG TCGCTTCTCT GTGTCCAGAA CTGAAGACAA GATGGCTGAG
    GTGAAGGGAG AGGAGCCAGT GGTCTCTCCT TTCATGGACT CAGAACACGA CATTCCTCCC
    ACCGTGACGC CAAAGAAAGA GAAACCCGAA CTGTCTGAGC CTTCGCATCT GAACGGGCCG
    TCTTCCGACC TGGAGGCTGC CTTCCTCAGC CGGGACGTGG ATGCTGGCTC AGGGAGCCCT
    CACTCCCCTC CGCAGCTGAG CTCCAAGAGC CTTCCTGCCC AGAGCCTCAG TCAGAGCCTC
    AGCAATTCCT TCAACTCTTC CTACATGAGC AGCGACAATG AGTCAGACAT TGAAGACGAG
    GACTTAAAGT TAGAGCTTCG CCGACTACGG GAAAAACATC TTAAAGAGAT TCAGGACCTG
    CAGAGTCGCC AGAAGCACGA AATTGAGTCT TTATACACCA AACTGGGCAA GGTTCCTCCT
    GCTGTCATCA TTCCCCCAGC AGCCCCCCTT GCAGGGAGAA GGAGGCGACC CACCAAAAGC
    AAAGGGAGCA AGTCCAGTCG CAGCAGCTCT TTGGGGAATA AAAGCCCTGG ACCAGGTACC
    CTGTCTGGCC AGAGTGCAGC TTCAGTCTTG CACCCTCAGC AGACCCTCCC CGCTCCTGGC
    AGCGTCCCGG AGACAGGGCC CAACCAGCTG TTACAGCCTG TGAAGCCATC TCCTTCCAGT
    GACAACCTGT ACTCGGCCTT TACCAGCGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCG
    CCAGGTCAAG GCTGTGCCAA GTTTAACTGT GCGTCCGAGC AGGTGACGTT CAAAGCTGGC
    GGCAGGAGGA CGCGATTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA
    GTCTGCCCTT GCAACCAGCT CTGTAGGACC AGCAGCACCA ACGCCGTTGG GGGAGCCGTG
    AGCAGCCAGG CCGCCCCGGC TCCACCTCCT GCCGTGACGT CCAGCAGGAA GGGCATGTTC
    ACGGACGACC TGCACAAGCT GGTGGACAAC TGGGCCCGGG ACGCCATGAG TCTCTCAGGC
    AGGCGGGGAA GCAAAGGACA CGTGAGCTAT GAGGGCCCTG GCATGGCAAG GAAGTTCTCT
    GCGCCCGGTC AGCTGTGCGT CTCCATGACC TCAAACCTGG GCGGCTCTGC CCCCGGCTCT
    GCAGCGTCAG CTACCTCTCT AGGGCACTTC CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC
    GGTTTCCCTA CCCCCCCGTT TGGCACGCAG TGGAGCGGGA CGGGTGGCCC GGCACCCCAG
    CCGCTTAGCC AGTTCCAGCC TGTGGGAACA GCCTCCTTAC AGAATTTCAA CATCAGCAAC
    TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848487.1
    CDS133..7323
    Translation

    Target ORF information:

    RefSeq Version XM_024992719.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992719.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCAA
    GGAGTGCAGC CAACAGCCCC CTCTCAGCAG GCGGTGCAGT ATCCGCTCCC TCAGACATCG
    GCCCCCAGCG AGGCCACGAT TGCACAGCCT GTGAGTCAGC CTCCACCTCC ACAGGCCTTG
    CCACACGTGT CCGCGGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TCTCCAAGGC
    GAACCTCAGA TCCCAGTTGC AACACAGCCC TCAGTCGTTC CCGTCCATGC TGGTGCTCAC
    TTCCTTCCTG TGGGACAGCC ACTCCCACCC TCTTTACTGC CCCAGTACCC CGTCTCTCAG
    CTGCCCCCCA TCTCTCAGCT CCCCTCTGCT CCTCCTCACG TGTCTGTGGC TCAGCCAGGC
    TTCCCACCCC TGCCGGTCAC GATGGCAGCT GGCGTTAGTC AGCCTCTGCT CACCTTGGCT
    TCATCTGCTG CGGCGGCGGC CGTCCCCGGG GCACCCACTG TGGTCCCTAG TCAGCTTCCT
    GCCCTCCTGC AGCCTGTGAC CCAGCTGCCG AGTCAGGCTC ACCCGCAGCT CCTGCAGCCA
    GCAGTTCAGT CCATGGGGAT CCCCGCCAGC CTTGGACAGA CTGCCGAGGT GCCACTTCCC
    CCTGGAGATA TTCTCTACCA GGGCTTCCCA CCTCGACTGC CATCGCAGTA CCCAGGAGAC
    TCAAACATTG CCCCCTCCTC CAGCGCAGCT TCTGTGTGCC TGCCTTCTGC AGTCCTGTCC
    CCTCCCATGC CGACAGAGGC GCTGGCTGCC CCGGGTTACT TTCCTGCAAT GGTGCAGCCC
    TATGTGGAAT CCAGTCTCTT GGTTCCTGTG GGCAGTGTAG GAGGACAGGT CCAGGTGTCC
    CAGCCGGCAG TGAGCTTAGC ACAAGCCCCC ACCACATCCT CCCAGCAAGC AGCTCTGGAG
    AGCACTCAGG TCGTCTCTCA GGTGGCTCCT CCAGAGCCAG CTCCCGGGGC TCAGCCACAG
    CCTGCCCAGC CAGCCGCGGT GGTCTCTTCT ATAGACAGCG CACATTCAGA TGTGGCTTCA
    GGCCTGAGTG ATGGCAACGA GAATGTCCCC TCATCCAGCG GGAGGCACGA GGGCAGGACC
    ACGAAGCGGC TGCATCGGAA GTCTATAAGG AGCCGCTCTC GTCACGAGAA GCCTTCCCGC
    CCCAAACTGA GAATTCTCAA TGTTTCAAAT AAGGGAGATC GGGTGGTAGA ATGTCAGTTA
    GAGACTCACA ATCGGAAGAT GGTTACATTC AAATTTGATT TAGATGGTGA CAACCCTGAG
    GAGATAGCCA CAATTATGGT GAACAATGAC TTTATTCTGG CACTGGAGAG AGATGCGTTT
    GTGGAGCAAG TGCGGGAGAT CATTGAGAAA GCTGACGAGA TGCTCAGTGA GGATGTCAGC
    GTGGAGCCAG AGGGTGACCA GAGCTTGGAG AGTCTCCATG GCAAGGATGA CTATGGCTTC
    TCAGGCACCC AGAAATTGGA AGGAGAGTTC AAACAACCAA TTCCTGCATC TTCCTTGCCA
    CAACAGATAG CAGGCATTCC TGCCAGTTCT TTAACTCAAG TTGTTCATTC TGCTGGAAGA
    CGGTTTATAG TAAGTCCTGT GCCAGAAAGT CGATTACGAG AATCAAAACT TTTCACTGAA
    ATTTCAGATA CGGTTGCTGC TTCCACATCT GCTGGCCCTG GAATGAACTT GTCTCACTCT
    GCCTCATCCC TTAGTCTGCA GCAGGCCTTT TCTGAACTTA GACATGCTCG GATGGCAGAA
    GGGCCGAGTA CGGCCCCTCC CCACTTTGGT CAGACAGGCC CCGTGTTTCC AGCAGTGCCT
    CCCACCCTGA GCAGTGCCCC TGGAGCCCCA GCCGCAGCAG CCGCAGCCTC GGTGTCAGCC
    CCTGCCCCAA GCCACCCTCT TAGTGACGTG ACCACATCGG TCATTCAGAC TGAGGTGACA
    GTGGCCCCTG GAACAGGTGT CATGCCTCCC AGTGGTCTCC CTGTACCCCC TGTGTCTGAG
    TCCCTGGCAC CTTCCAGCGT GGCCCCCAGC GTCACCATCC CTGCAGTTCT CTCGGCACTA
    CCAGCGCCCC AGGCGGCACA GGCTCCCACC CCTGGCAGCG GGGCCTCCAG TGCAGGCACT
    TTCCCCTCTG CACCCGCATC CCTGACACCA GCCTCTGCTG TGGGCAGCGC TGTTGCCTCT
    GCTGCTCAGC TTCCGCCTGT GCCGTCTCAG CCCGCGGTAG GCAGCTCGAC CGGGGTGGCC
    ACGCTGACAG CCGTGCCCGC TGCCGCTCTG GGCCCTAGCC TCACGGCACT GCCGTCCCTT
    GCTCTCAGCA GCAGTACCTC TGCCCCAGCT CTGGCAGAGA CAGTGCTGGT CAGTGCCCAC
    GCACTGGATA AGACGGCCCA TAGTAGCACC ACTGGCCTGG CTCTGTCCCT TCCTGCCACC
    TCGTCCTCTC CCGCCCCTGG AGCAGGAGTG TCCAGTGCTG TTTCTCAGCC TACTGCTGCG
    GCTCACCCTT TGGTCCTCCC ATCAGTCGTA ACCTCTACTC CCGTACTCTC CCAGGCAGGA
    CCCATGTCAG CACCTCTACT GCCCCAGGTA CCCGGTATTC CACCCCTGGT ACAACCTGTT
    GTCAGCGTGC CTGCTGTGCA ACAGACCCTG GTTCATAGTC AGCCTCAGCC CGCTCCACTC
    CCCAACCAGC CCCACACTCA CTGTCCCGAG ATAGACGCCG ACACTCAACC CAAAGCTCCC
    GGGATCGACG ACATCAAGAC TTTGGAGGAA AAGCTGCGAT CTCTCTTTAG TGAGCACAGT
    TCTTCTGGAG CCCAACACGC CCCCGTCTCT CTGGAGACCT CGCTCACTGT AGAGACCGCT
    GTCACAGCAG GCCTCCCAAC CACTGCTGTT GCACCAAGCA AGCCTCTGAC TTCCACTGCC
    AGTACTTGCT TGCCACCAAC CAGTCTGCCC TTAGGAGCAG CTGGCTTGTC AGTTATACCA
    ATGGTCACTC CTGGTCAAGT TTCCACCCCA GTCAGCTCTG CGCCAGGAGT GAAACTTGAA
    ACTGCTCCAG CAAAGCCACC TTTAACTAAG CCTCCAGTGC TGCCCATGGG CACTGAACTT
    CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG
    CCCCCGCAGC CCCTGGGAGA CCTGGACGCT CAGCTGAGAA GAACCCTCAG TCCCGAGAGT
    GGCATGCTGA CGTCTGCCGT TGGACCCGTG TCTGTGGTGG CTCCAACAGC AGTTGTGGAG
    ACCGAAGCCC AACTTCCAAA GGATGTTCTT TCAGTCTCTC AAGTCACAGA AGCTCCTGTC
    CTTGCGCCCA CTTCCACAGC TGGTGTTCTG AAGATGGGGC GATTTCAGGT CTCAGTTGCA
    ATGGATGACC CCCAGAAGGA GGGTAAAAGT AAGTCGGAGG ATGCCAAGTC TGTCCACTTC
    GCATCCAGCA CGTCAGAGTC CTCGGTGCTG TCAAGCAGCA GCCCCGAGAG CACCGTGGTG
    AAAGCAGAGC CCAACGGGCT GGCCGTCCGG GGCGCCTCTG CAGACATGCC TGACAGCGCC
    CACCAGCCAC CTGCCTCAGA AGCTGGGCAG CCTAGCAAGG TGGGCCGTTT TCAGGTGACC
    ACCACAGCGA GCAGAGTGGG TCGCTTCTCT GTGTCCAGAA CTGAAGACAA GATGGCTGAG
    GTGAAGGGAG AGGAGCCAGT GGTCTCTCCT TTCATGGACT CAGAACACGA CATTCCTCCC
    ACCGTGACGC CAAAGAAAGA GAAACCCGAA CTGTCTGAGC CTTCGCATCT GAACGGGCCG
    TCTTCCGACC TGGAGGCTGC CTTCCTCAGC CGGGACGTGG ATGCTGGCTC AGGGAGCCCT
    CACTCCCCTC CGCAGCTGAG CTCCAAGAGC CTTCCTGCCC AGAGCCTCAG TCAGAGCCTC
    AGCAATTCCT TCAACTCTTC CTACATGAGC AGCGACAATG AGTCAGACAT TGAAGACGAG
    GACTTAAAGT TAGAGCTTCG CCGACTACGG GAAAAACATC TTAAAGAGAT TCAGGACCTG
    CAGAGTCGCC AGAAGCACGA AATTGAGTCT TTATACACCA AACTGGGCAA GGTTCCTCCT
    GCTGTCATCA TTCCCCCAGC AGCCCCCCTT GCAGGGAGAA GGAGGCGACC CACCAAAAGC
    AAAGGGAGCA AGTCCAGTCG CAGCAGCTCT TTGGGGAATA AAAGCCCTGG ACCAGGTACC
    CTGTCTGGCC AGAGTGCAGC TTCAGTCTTG CACCCTCAGC AGACCCTCCC CGCTCCTGGC
    AGCGTCCCGG AGACAGGGCC CAACCAGCTG TTACAGCCTG TGAAGCCATC TCCTTCCAGT
    GACAACCTGT ACTCGGCCTT TACCAGCGAT GGTGCCATTT CAGTACCAAG CCTTTCTGCG
    CCAGGTCAAG GCTGTGCCAA GTTTAACTGT GCGTCCGAGC AGGTGACGTT CAAAGCTGGC
    GGCAGGAGGA CGCGATTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA
    GTCTGCCCTT GCAACCAGCT CTGTAGGACC AGCAGCACCA ACGCCGTTGG GGGAGCCGTG
    AGCAGCCAGG CCGCCCCGGC TCCACCTCCT GCCGTGACGT CCAGCAGGAA GGGCATGTTC
    ACGGACGACC TGCACAAGCT GGTGGACAAC TGGGCCCGGG ACGCCATGAG TCTCTCAGGC
    AGGCGGGGAA GCAAAGGACA CGTGAGCTAT GAGGGCCCTG GCATGGCAAG GAAGTTCTCT
    GCGCCCGGTC AGCTGTGCGT CTCCATGACC TCAAACCTGG GCGGCTCTGC CCCCGGCTCT
    GCAGCGTCAG CTACCTCTCT AGGGCACTTC CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC
    GGTTTCCCTA CCCCCCCGTT TGGCACGCAG TGGAGCGGGA CGGGTGGCCC GGCACCCCAG
    CCGCTTAGCC AGTTCCAGCC TGTGGGAACA GCCTCCTTAC AGAATTTCAA CATCAGCAAC
    TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234919
    Clone ID Related Accession (Same CDS sequence) XM_024992720.1
    Accession Version XM_024992720.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7185bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGGA AGATGGTGAA AAAAGTCTGC
    CCTTGCAACC AGCTCTGTAG GACCAGCAGC ACCAACGCCG TTGGGGGAGC CGTGAGCAGC
    CAGGCCGCCC CGGCTCCACC TCCTGCCGTG ACGTCCAGCA GGAAGGGCAT GTTCACGGAC
    GACCTGCACA AGCTGGTGGA CAACTGGGCC CGGGACGCCA TGAGTCTCTC AGGCAGGCGG
    GGAAGCAAAG GACACGTGAG CTATGAGGGC CCTGGCATGG CAAGGAAGTT CTCTGCGCCC
    GGTCAGCTGT GCGTCTCCAT GACCTCAAAC CTGGGCGGCT CTGCCCCCGG CTCTGCAGCG
    TCAGCTACCT CTCTAGGGCA CTTCCCCAAG TCCATGTGCC CCCCTCAGCA GTACGGTTTC
    CCTACCCCCC CGTTTGGCAC GCAGTGGAGC GGGACGGGTG GCCCGGCACC CCAGCCGCTT
    AGCCAGTTCC AGCCTGTGGG AACAGCCTCC TTACAGAATT TCAACATCAG CAACTTGCAG
    AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848488.1
    CDS133..7317
    Translation

    Target ORF information:

    RefSeq Version XM_024992720.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992720.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGGA AGATGGTGAA AAAAGTCTGC
    CCTTGCAACC AGCTCTGTAG GACCAGCAGC ACCAACGCCG TTGGGGGAGC CGTGAGCAGC
    CAGGCCGCCC CGGCTCCACC TCCTGCCGTG ACGTCCAGCA GGAAGGGCAT GTTCACGGAC
    GACCTGCACA AGCTGGTGGA CAACTGGGCC CGGGACGCCA TGAGTCTCTC AGGCAGGCGG
    GGAAGCAAAG GACACGTGAG CTATGAGGGC CCTGGCATGG CAAGGAAGTT CTCTGCGCCC
    GGTCAGCTGT GCGTCTCCAT GACCTCAAAC CTGGGCGGCT CTGCCCCCGG CTCTGCAGCG
    TCAGCTACCT CTCTAGGGCA CTTCCCCAAG TCCATGTGCC CCCCTCAGCA GTACGGTTTC
    CCTACCCCCC CGTTTGGCAC GCAGTGGAGC GGGACGGGTG GCCCGGCACC CCAGCCGCTT
    AGCCAGTTCC AGCCTGTGGG AACAGCCTCC TTACAGAATT TCAACATCAG CAACTTGCAG
    AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234920
    Clone ID Related Accession (Same CDS sequence) XM_024992721.1
    Accession Version XM_024992721.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGGA CCAGCAGCAC CAACGCCGTT
    GGGGGAGCCG TGAGCAGCCA GGCCGCCCCG GCTCCACCTC CTGCCGTGAC GTCCAGCAGG
    AAGGGCATGT TCACGGACGA CCTGCACAAG CTGGTGGACA ACTGGGCCCG GGACGCCATG
    AGTCTCTCAG GCAGGCGGGG AAGCAAAGGA CACGTGAGCT ATGAGGGCCC TGGCATGGCA
    AGGAAGTTCT CTGCGCCCGG TCAGCTGTGC GTCTCCATGA CCTCAAACCT GGGCGGCTCT
    GCCCCCGGCT CTGCAGCGTC AGCTACCTCT CTAGGGCACT TCCCCAAGTC CATGTGCCCC
    CCTCAGCAGT ACGGTTTCCC TACCCCCCCG TTTGGCACGC AGTGGAGCGG GACGGGTGGC
    CCGGCACCCC AGCCGCTTAG CCAGTTCCAG CCTGTGGGAA CAGCCTCCTT ACAGAATTTC
    AACATCAGCA ACTTGCAGAA ATCCATCAGC AACCCCCCAG GTTCCAACCT GCGGACCACG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848489.1
    CDS132..7274
    Translation

    Target ORF information:

    RefSeq Version XM_024992721.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992721.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGGA CCAGCAGCAC CAACGCCGTT
    GGGGGAGCCG TGAGCAGCCA GGCCGCCCCG GCTCCACCTC CTGCCGTGAC GTCCAGCAGG
    AAGGGCATGT TCACGGACGA CCTGCACAAG CTGGTGGACA ACTGGGCCCG GGACGCCATG
    AGTCTCTCAG GCAGGCGGGG AAGCAAAGGA CACGTGAGCT ATGAGGGCCC TGGCATGGCA
    AGGAAGTTCT CTGCGCCCGG TCAGCTGTGC GTCTCCATGA CCTCAAACCT GGGCGGCTCT
    GCCCCCGGCT CTGCAGCGTC AGCTACCTCT CTAGGGCACT TCCCCAAGTC CATGTGCCCC
    CCTCAGCAGT ACGGTTTCCC TACCCCCCCG TTTGGCACGC AGTGGAGCGG GACGGGTGGC
    CCGGCACCCC AGCCGCTTAG CCAGTTCCAG CCTGTGGGAA CAGCCTCCTT ACAGAATTTC
    AACATCAGCA ACTTGCAGAA ATCCATCAGC AACCCCCCAG GTTCCAACCT GCGGACCACG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234921
    Clone ID Related Accession (Same CDS sequence) XM_024992722.1
    Accession Version XM_024992722.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCACCCCCGC AGCCCCTGGG AGACCTGGAC GCTCAGCTGA GAAGAACCCT CAGTCCCGAG
    AGTGGCATGC TGACGTCTGC CGTTGGACCC GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG
    GAGACCGAAG CCCAACTTCC AAAGGATGTT CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT
    GTCCTTGCGC CCACTTCCAC AGCTGGTGTT CTGAAGATGG GGCGATTTCA GGTCTCAGTT
    GCAATGGATG ACCCCCAGAA GGAGGGTAAA AGTAAGTCGG AGGATGCCAA GTCTGTCCAC
    TTCGCATCCA GCACGTCAGA GTCCTCGGTG CTGTCAAGCA GCAGCCCCGA GAGCACCGTG
    GTGAAAGCAG AGCCCAACGG GCTGGCCGTC CGGGGCGCCT CTGCAGACAT GCCTGACAGC
    GCCCACCAGC CACCTGCCTC AGAAGCTGGG CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG
    ACCACCACAG CGAGCAGAGT GGGTCGCTTC TCTGTGTCCA GAACTGAAGA CAAGATGGCT
    GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT CCTTTCATGG ACTCAGAACA CGACATTCCT
    CCCACCGTGA CGCCAAAGAA AGAGAAACCC GAACTGTCTG AGCCTTCGCA TCTGAACGGG
    CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC AGCCGGGACG TGGATGCTGG CTCAGGGAGC
    CCTCACTCCC CTCCGCAGCT GAGCTCCAAG AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC
    CTCAGCAATT CCTTCAACTC TTCCTACATG AGCAGCGACA ATGAGTCAGA CATTGAAGAC
    GAGGACTTAA AGTTAGAGCT TCGCCGACTA CGGGAAAAAC ATCTTAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA CGAAATTGAG TCTTTATACA CCAAACTGGG CAAGGTTCCT
    CCTGCTGTCA TCATTCCCCC AGCAGCCCCC CTTGCAGGGA GAAGGAGGCG ACCCACCAAA
    AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC TCTTTGGGGA ATAAAAGCCC TGGACCAGGT
    ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC TTGCACCCTC AGCAGACCCT CCCCGCTCCT
    GGCAGCGTCC CGGAGACAGG GCCCAACCAG CTGTTACAGC CTGTGAAGCC ATCTCCTTCC
    AGTGACAACC TGTACTCGGC CTTTACCAGC GATGGTGCCA TTTCAGTACC AAGCCTTTCT
    GCGCCAGGTC AAGGGACCAG CAGCACCAAC GCCGTTGGGG GAGCCGTGAG CAGCCAGGCC
    GCCCCGGCTC CACCTCCTGC CGTGACGTCC AGCAGGAAGG GCATGTTCAC GGACGACCTG
    CACAAGCTGG TGGACAACTG GGCCCGGGAC GCCATGAGTC TCTCAGGCAG GCGGGGAAGC
    AAAGGACACG TGAGCTATGA GGGCCCTGGC ATGGCAAGGA AGTTCTCTGC GCCCGGTCAG
    CTGTGCGTCT CCATGACCTC AAACCTGGGC GGCTCTGCCC CCGGCTCTGC AGCGTCAGCT
    ACCTCTCTAG GGCACTTCCC CAAGTCCATG TGCCCCCCTC AGCAGTACGG TTTCCCTACC
    CCCCCGTTTG GCACGCAGTG GAGCGGGACG GGTGGCCCGG CACCCCAGCC GCTTAGCCAG
    TTCCAGCCTG TGGGAACAGC CTCCTTACAG AATTTCAACA TCAGCAACTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848490.1
    CDS132..7190
    Translation

    Target ORF information:

    RefSeq Version XM_024992722.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992722.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGGG CTTCCCACCT
    CGACTGCCAT CGCAGTACCC AGGAGACTCA AACATTGCCC CCTCCTCCAG CGCAGCTTCT
    GTGTGCCTGC CTTCTGCAGT CCTGTCCCCT CCCATGCCGA CAGAGGCGCT GGCTGCCCCG
    GGTTACTTTC CTGCAATGGT GCAGCCCTAT GTGGAATCCA GTCTCTTGGT TCCTGTGGGC
    AGTGTAGGAG GACAGGTCCA GGTGTCCCAG CCGGCAGTGA GCTTAGCACA AGCCCCCACC
    ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGTCG TCTCTCAGGT GGCTCCTCCA
    GAGCCAGCTC CCGGGGCTCA GCCACAGCCT GCCCAGCCAG CCGCGGTGGT CTCTTCTATA
    GACAGCGCAC ATTCAGATGT GGCTTCAGGC CTGAGTGATG GCAACGAGAA TGTCCCCTCA
    TCCAGCGGGA GGCACGAGGG CAGGACCACG AAGCGGCTGC ATCGGAAGTC TATAAGGAGC
    CGCTCTCGTC ACGAGAAGCC TTCCCGCCCC AAACTGAGAA TTCTCAATGT TTCAAATAAG
    GGAGATCGGG TGGTAGAATG TCAGTTAGAG ACTCACAATC GGAAGATGGT TACATTCAAA
    TTTGATTTAG ATGGTGACAA CCCTGAGGAG ATAGCCACAA TTATGGTGAA CAATGACTTT
    ATTCTGGCAC TGGAGAGAGA TGCGTTTGTG GAGCAAGTGC GGGAGATCAT TGAGAAAGCT
    GACGAGATGC TCAGTGAGGA TGTCAGCGTG GAGCCAGAGG GTGACCAGAG CTTGGAGAGT
    CTCCATGGCA AGGATGACTA TGGCTTCTCA GGCACCCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCATCTTC CTTGCCACAA CAGATAGCAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCACCCCCGC AGCCCCTGGG AGACCTGGAC GCTCAGCTGA GAAGAACCCT CAGTCCCGAG
    AGTGGCATGC TGACGTCTGC CGTTGGACCC GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG
    GAGACCGAAG CCCAACTTCC AAAGGATGTT CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT
    GTCCTTGCGC CCACTTCCAC AGCTGGTGTT CTGAAGATGG GGCGATTTCA GGTCTCAGTT
    GCAATGGATG ACCCCCAGAA GGAGGGTAAA AGTAAGTCGG AGGATGCCAA GTCTGTCCAC
    TTCGCATCCA GCACGTCAGA GTCCTCGGTG CTGTCAAGCA GCAGCCCCGA GAGCACCGTG
    GTGAAAGCAG AGCCCAACGG GCTGGCCGTC CGGGGCGCCT CTGCAGACAT GCCTGACAGC
    GCCCACCAGC CACCTGCCTC AGAAGCTGGG CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG
    ACCACCACAG CGAGCAGAGT GGGTCGCTTC TCTGTGTCCA GAACTGAAGA CAAGATGGCT
    GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT CCTTTCATGG ACTCAGAACA CGACATTCCT
    CCCACCGTGA CGCCAAAGAA AGAGAAACCC GAACTGTCTG AGCCTTCGCA TCTGAACGGG
    CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC AGCCGGGACG TGGATGCTGG CTCAGGGAGC
    CCTCACTCCC CTCCGCAGCT GAGCTCCAAG AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC
    CTCAGCAATT CCTTCAACTC TTCCTACATG AGCAGCGACA ATGAGTCAGA CATTGAAGAC
    GAGGACTTAA AGTTAGAGCT TCGCCGACTA CGGGAAAAAC ATCTTAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA CGAAATTGAG TCTTTATACA CCAAACTGGG CAAGGTTCCT
    CCTGCTGTCA TCATTCCCCC AGCAGCCCCC CTTGCAGGGA GAAGGAGGCG ACCCACCAAA
    AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC TCTTTGGGGA ATAAAAGCCC TGGACCAGGT
    ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC TTGCACCCTC AGCAGACCCT CCCCGCTCCT
    GGCAGCGTCC CGGAGACAGG GCCCAACCAG CTGTTACAGC CTGTGAAGCC ATCTCCTTCC
    AGTGACAACC TGTACTCGGC CTTTACCAGC GATGGTGCCA TTTCAGTACC AAGCCTTTCT
    GCGCCAGGTC AAGGGACCAG CAGCACCAAC GCCGTTGGGG GAGCCGTGAG CAGCCAGGCC
    GCCCCGGCTC CACCTCCTGC CGTGACGTCC AGCAGGAAGG GCATGTTCAC GGACGACCTG
    CACAAGCTGG TGGACAACTG GGCCCGGGAC GCCATGAGTC TCTCAGGCAG GCGGGGAAGC
    AAAGGACACG TGAGCTATGA GGGCCCTGGC ATGGCAAGGA AGTTCTCTGC GCCCGGTCAG
    CTGTGCGTCT CCATGACCTC AAACCTGGGC GGCTCTGCCC CCGGCTCTGC AGCGTCAGCT
    ACCTCTCTAG GGCACTTCCC CAAGTCCATG TGCCCCCCTC AGCAGTACGG TTTCCCTACC
    CCCCCGTTTG GCACGCAGTG GAGCGGGACG GGTGGCCCGG CACCCCAGCC GCTTAGCCAG
    TTCCAGCCTG TGGGAACAGC CTCCTTACAG AATTTCAACA TCAGCAACTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234922
    Clone ID Related Accession (Same CDS sequence) XM_024992723.1
    Accession Version XM_024992723.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGAG CACTCAGGTC
    GTCTCTCAGG TGGCTCCTCC AGAGCCAGCT CCCGGGGCTC AGCCACAGCC TGCCCAGCCA
    GCCGCGGTGG TCTCTTCTAT AGACAGCGCA CATTCAGATG TGGCTTCAGG CCTGAGTGAT
    GGCAACGAGA ATGTCCCCTC ATCCAGCGGG AGGCACGAGG GCAGGACCAC GAAGCGGCTG
    CATCGGAAGT CTATAAGGAG CCGCTCTCGT CACGAGAAGC CTTCCCGCCC CAAACTGAGA
    ATTCTCAATG TTTCAAATAA GGGAGATCGG GTGGTAGAAT GTCAGTTAGA GACTCACAAT
    CGGAAGATGG TTACATTCAA ATTTGATTTA GATGGTGACA ACCCTGAGGA GATAGCCACA
    ATTATGGTGA ACAATGACTT TATTCTGGCA CTGGAGAGAG ATGCGTTTGT GGAGCAAGTG
    CGGGAGATCA TTGAGAAAGC TGACGAGATG CTCAGTGAGG ATGTCAGCGT GGAGCCAGAG
    GGTGACCAGA GCTTGGAGAG TCTCCATGGC AAGGATGACT ATGGCTTCTC AGGCACCCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCATCTT CCTTGCCACA ACAGATAGCA
    GGCATTCCTG CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTA
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAACTTT TCACTGAAAT TTCAGATACG
    GTTGCTGCTT CCACATCTGC TGGCCCTGGA ATGAACTTGT CTCACTCTGC CTCATCCCTT
    AGTCTGCAGC AGGCCTTTTC TGAACTTAGA CATGCTCGGA TGGCAGAAGG GCCGAGTACG
    GCCCCTCCCC ACTTTGGTCA GACAGGCCCC GTGTTTCCAG CAGTGCCTCC CACCCTGAGC
    AGTGCCCCTG GAGCCCCAGC CGCAGCAGCC GCAGCCTCGG TGTCAGCCCC TGCCCCAAGC
    CACCCTCTTA GTGACGTGAC CACATCGGTC ATTCAGACTG AGGTGACAGT GGCCCCTGGA
    ACAGGTGTCA TGCCTCCCAG TGGTCTCCCT GTACCCCCTG TGTCTGAGTC CCTGGCACCT
    TCCAGCGTGG CCCCCAGCGT CACCATCCCT GCAGTTCTCT CGGCACTACC AGCGCCCCAG
    GCGGCACAGG CTCCCACCCC TGGCAGCGGG GCCTCCAGTG CAGGCACTTT CCCCTCTGCA
    CCCGCATCCC TGACACCAGC CTCTGCTGTG GGCAGCGCTG TTGCCTCTGC TGCTCAGCTT
    CCGCCTGTGC CGTCTCAGCC CGCGGTAGGC AGCTCGACCG GGGTGGCCAC GCTGACAGCC
    GTGCCCGCTG CCGCTCTGGG CCCTAGCCTC ACGGCACTGC CGTCCCTTGC TCTCAGCAGC
    AGTACCTCTG CCCCAGCTCT GGCAGAGACA GTGCTGGTCA GTGCCCACGC ACTGGATAAG
    ACGGCCCATA GTAGCACCAC TGGCCTGGCT CTGTCCCTTC CTGCCACCTC GTCCTCTCCC
    GCCCCTGGAG CAGGAGTGTC CAGTGCTGTT TCTCAGCCTA CTGCTGCGGC TCACCCTTTG
    GTCCTCCCAT CAGTCGTAAC CTCTACTCCC GTACTCTCCC AGGCAGGACC CATGTCAGCA
    CCTCTACTGC CCCAGGTACC CGGTATTCCA CCCCTGGTAC AACCTGTTGT CAGCGTGCCT
    GCTGTGCAAC AGACCCTGGT TCATAGTCAG CCTCAGCCCG CTCCACTCCC CAACCAGCCC
    CACACTCACT GTCCCGAGAT AGACGCCGAC ACTCAACCCA AAGCTCCCGG GATCGACGAC
    ATCAAGACTT TGGAGGAAAA GCTGCGATCT CTCTTTAGTG AGCACAGTTC TTCTGGAGCC
    CAACACGCCC CCGTCTCTCT GGAGACCTCG CTCACTGTAG AGACCGCTGT CACAGCAGGC
    CTCCCAACCA CTGCTGTTGC ACCAAGCAAG CCTCTGACTT CCACTGCCAG TACTTGCTTG
    CCACCAACCA GTCTGCCCTT AGGAGCAGCT GGCTTGTCAG TTATACCAAT GGTCACTCCT
    GGTCAAGTTT CCACCCCAGT CAGCTCTGCG CCAGGAGTGA AACTTGAAAC TGCTCCAGCA
    AAGCCACCTT TAACTAAGCC TCCAGTGCTG CCCATGGGCA CTGAACTTCC AGCTGGTACT
    CCACCCAGCG AGCAGCTGCC ACCTTTTCCA GGACCTTCAC TAACTCAGCC CCCGCAGCCC
    CTGGGAGACC TGGACGCTCA GCTGAGAAGA ACCCTCAGTC CCGAGAGTGG CATGCTGACG
    TCTGCCGTTG GACCCGTGTC TGTGGTGGCT CCAACAGCAG TTGTGGAGAC CGAAGCCCAA
    CTTCCAAAGG ATGTTCTTTC AGTCTCTCAA GTCACAGAAG CTCCTGTCCT TGCGCCCACT
    TCCACAGCTG GTGTTCTGAA GATGGGGCGA TTTCAGGTCT CAGTTGCAAT GGATGACCCC
    CAGAAGGAGG GTAAAAGTAA GTCGGAGGAT GCCAAGTCTG TCCACTTCGC ATCCAGCACG
    TCAGAGTCCT CGGTGCTGTC AAGCAGCAGC CCCGAGAGCA CCGTGGTGAA AGCAGAGCCC
    AACGGGCTGG CCGTCCGGGG CGCCTCTGCA GACATGCCTG ACAGCGCCCA CCAGCCACCT
    GCCTCAGAAG CTGGGCAGCC TAGCAAGGTG GGCCGTTTTC AGGTGACCAC CACAGCGAGC
    AGAGTGGGTC GCTTCTCTGT GTCCAGAACT GAAGACAAGA TGGCTGAGGT GAAGGGAGAG
    GAGCCAGTGG TCTCTCCTTT CATGGACTCA GAACACGACA TTCCTCCCAC CGTGACGCCA
    AAGAAAGAGA AACCCGAACT GTCTGAGCCT TCGCATCTGA ACGGGCCGTC TTCCGACCTG
    GAGGCTGCCT TCCTCAGCCG GGACGTGGAT GCTGGCTCAG GGAGCCCTCA CTCCCCTCCG
    CAGCTGAGCT CCAAGAGCCT TCCTGCCCAG AGCCTCAGTC AGAGCCTCAG CAATTCCTTC
    AACTCTTCCT ACATGAGCAG CGACAATGAG TCAGACATTG AAGACGAGGA CTTAAAGTTA
    GAGCTTCGCC GACTACGGGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCACGAAA TTGAGTCTTT ATACACCAAA CTGGGCAAGG TTCCTCCTGC TGTCATCATT
    CCCCCAGCAG CCCCCCTTGC AGGGAGAAGG AGGCGACCCA CCAAAAGCAA AGGGAGCAAG
    TCCAGTCGCA GCAGCTCTTT GGGGAATAAA AGCCCTGGAC CAGGTACCCT GTCTGGCCAG
    AGTGCAGCTT CAGTCTTGCA CCCTCAGCAG ACCCTCCCCG CTCCTGGCAG CGTCCCGGAG
    ACAGGGCCCA ACCAGCTGTT ACAGCCTGTG AAGCCATCTC CTTCCAGTGA CAACCTGTAC
    TCGGCCTTTA CCAGCGATGG TGCCATTTCA GTACCAAGCC TTTCTGCGCC AGGTCAAGGC
    TGTGCCAAGT TTAACTGTGC GTCCGAGCAG GTGACGTTCA AAGCTGGCGG CAGGAGGACG
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCTTGC
    AACCAGCTCT GTAGGACCAG CAGCACCAAC GCCGTTGGGG GAGCCGTGAG CAGCCAGGCC
    GCCCCGGCTC CACCTCCTGC CGTGACGTCC AGCAGGAAGG GCATGTTCAC GGACGACCTG
    CACAAGCTGG TGGACAACTG GGCCCGGGAC GCCATGAGTC TCTCAGGCAG GCGGGGAAGC
    AAAGGACACG TGAGCTATGA GGGCCCTGGC ATGGCAAGGA AGTTCTCTGC GCCCGGTCAG
    CTGTGCGTCT CCATGACCTC AAACCTGGGC GGCTCTGCCC CCGGCTCTGC AGCGTCAGCT
    ACCTCTCTAG GGCACTTCCC CAAGTCCATG TGCCCCCCTC AGCAGTACGG TTTCCCTACC
    CCCCCGTTTG GCACGCAGTG GAGCGGGACG GGTGGCCCGG CACCCCAGCC GCTTAGCCAG
    TTCCAGCCTG TGGGAACAGC CTCCTTACAG AATTTCAACA TCAGCAACTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848491.1
    CDS132..7130
    Translation

    Target ORF information:

    RefSeq Version XM_024992723.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992723.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGAG CACTCAGGTC
    GTCTCTCAGG TGGCTCCTCC AGAGCCAGCT CCCGGGGCTC AGCCACAGCC TGCCCAGCCA
    GCCGCGGTGG TCTCTTCTAT AGACAGCGCA CATTCAGATG TGGCTTCAGG CCTGAGTGAT
    GGCAACGAGA ATGTCCCCTC ATCCAGCGGG AGGCACGAGG GCAGGACCAC GAAGCGGCTG
    CATCGGAAGT CTATAAGGAG CCGCTCTCGT CACGAGAAGC CTTCCCGCCC CAAACTGAGA
    ATTCTCAATG TTTCAAATAA GGGAGATCGG GTGGTAGAAT GTCAGTTAGA GACTCACAAT
    CGGAAGATGG TTACATTCAA ATTTGATTTA GATGGTGACA ACCCTGAGGA GATAGCCACA
    ATTATGGTGA ACAATGACTT TATTCTGGCA CTGGAGAGAG ATGCGTTTGT GGAGCAAGTG
    CGGGAGATCA TTGAGAAAGC TGACGAGATG CTCAGTGAGG ATGTCAGCGT GGAGCCAGAG
    GGTGACCAGA GCTTGGAGAG TCTCCATGGC AAGGATGACT ATGGCTTCTC AGGCACCCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCATCTT CCTTGCCACA ACAGATAGCA
    GGCATTCCTG CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTA
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAACTTT TCACTGAAAT TTCAGATACG
    GTTGCTGCTT CCACATCTGC TGGCCCTGGA ATGAACTTGT CTCACTCTGC CTCATCCCTT
    AGTCTGCAGC AGGCCTTTTC TGAACTTAGA CATGCTCGGA TGGCAGAAGG GCCGAGTACG
    GCCCCTCCCC ACTTTGGTCA GACAGGCCCC GTGTTTCCAG CAGTGCCTCC CACCCTGAGC
    AGTGCCCCTG GAGCCCCAGC CGCAGCAGCC GCAGCCTCGG TGTCAGCCCC TGCCCCAAGC
    CACCCTCTTA GTGACGTGAC CACATCGGTC ATTCAGACTG AGGTGACAGT GGCCCCTGGA
    ACAGGTGTCA TGCCTCCCAG TGGTCTCCCT GTACCCCCTG TGTCTGAGTC CCTGGCACCT
    TCCAGCGTGG CCCCCAGCGT CACCATCCCT GCAGTTCTCT CGGCACTACC AGCGCCCCAG
    GCGGCACAGG CTCCCACCCC TGGCAGCGGG GCCTCCAGTG CAGGCACTTT CCCCTCTGCA
    CCCGCATCCC TGACACCAGC CTCTGCTGTG GGCAGCGCTG TTGCCTCTGC TGCTCAGCTT
    CCGCCTGTGC CGTCTCAGCC CGCGGTAGGC AGCTCGACCG GGGTGGCCAC GCTGACAGCC
    GTGCCCGCTG CCGCTCTGGG CCCTAGCCTC ACGGCACTGC CGTCCCTTGC TCTCAGCAGC
    AGTACCTCTG CCCCAGCTCT GGCAGAGACA GTGCTGGTCA GTGCCCACGC ACTGGATAAG
    ACGGCCCATA GTAGCACCAC TGGCCTGGCT CTGTCCCTTC CTGCCACCTC GTCCTCTCCC
    GCCCCTGGAG CAGGAGTGTC CAGTGCTGTT TCTCAGCCTA CTGCTGCGGC TCACCCTTTG
    GTCCTCCCAT CAGTCGTAAC CTCTACTCCC GTACTCTCCC AGGCAGGACC CATGTCAGCA
    CCTCTACTGC CCCAGGTACC CGGTATTCCA CCCCTGGTAC AACCTGTTGT CAGCGTGCCT
    GCTGTGCAAC AGACCCTGGT TCATAGTCAG CCTCAGCCCG CTCCACTCCC CAACCAGCCC
    CACACTCACT GTCCCGAGAT AGACGCCGAC ACTCAACCCA AAGCTCCCGG GATCGACGAC
    ATCAAGACTT TGGAGGAAAA GCTGCGATCT CTCTTTAGTG AGCACAGTTC TTCTGGAGCC
    CAACACGCCC CCGTCTCTCT GGAGACCTCG CTCACTGTAG AGACCGCTGT CACAGCAGGC
    CTCCCAACCA CTGCTGTTGC ACCAAGCAAG CCTCTGACTT CCACTGCCAG TACTTGCTTG
    CCACCAACCA GTCTGCCCTT AGGAGCAGCT GGCTTGTCAG TTATACCAAT GGTCACTCCT
    GGTCAAGTTT CCACCCCAGT CAGCTCTGCG CCAGGAGTGA AACTTGAAAC TGCTCCAGCA
    AAGCCACCTT TAACTAAGCC TCCAGTGCTG CCCATGGGCA CTGAACTTCC AGCTGGTACT
    CCACCCAGCG AGCAGCTGCC ACCTTTTCCA GGACCTTCAC TAACTCAGCC CCCGCAGCCC
    CTGGGAGACC TGGACGCTCA GCTGAGAAGA ACCCTCAGTC CCGAGAGTGG CATGCTGACG
    TCTGCCGTTG GACCCGTGTC TGTGGTGGCT CCAACAGCAG TTGTGGAGAC CGAAGCCCAA
    CTTCCAAAGG ATGTTCTTTC AGTCTCTCAA GTCACAGAAG CTCCTGTCCT TGCGCCCACT
    TCCACAGCTG GTGTTCTGAA GATGGGGCGA TTTCAGGTCT CAGTTGCAAT GGATGACCCC
    CAGAAGGAGG GTAAAAGTAA GTCGGAGGAT GCCAAGTCTG TCCACTTCGC ATCCAGCACG
    TCAGAGTCCT CGGTGCTGTC AAGCAGCAGC CCCGAGAGCA CCGTGGTGAA AGCAGAGCCC
    AACGGGCTGG CCGTCCGGGG CGCCTCTGCA GACATGCCTG ACAGCGCCCA CCAGCCACCT
    GCCTCAGAAG CTGGGCAGCC TAGCAAGGTG GGCCGTTTTC AGGTGACCAC CACAGCGAGC
    AGAGTGGGTC GCTTCTCTGT GTCCAGAACT GAAGACAAGA TGGCTGAGGT GAAGGGAGAG
    GAGCCAGTGG TCTCTCCTTT CATGGACTCA GAACACGACA TTCCTCCCAC CGTGACGCCA
    AAGAAAGAGA AACCCGAACT GTCTGAGCCT TCGCATCTGA ACGGGCCGTC TTCCGACCTG
    GAGGCTGCCT TCCTCAGCCG GGACGTGGAT GCTGGCTCAG GGAGCCCTCA CTCCCCTCCG
    CAGCTGAGCT CCAAGAGCCT TCCTGCCCAG AGCCTCAGTC AGAGCCTCAG CAATTCCTTC
    AACTCTTCCT ACATGAGCAG CGACAATGAG TCAGACATTG AAGACGAGGA CTTAAAGTTA
    GAGCTTCGCC GACTACGGGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCACGAAA TTGAGTCTTT ATACACCAAA CTGGGCAAGG TTCCTCCTGC TGTCATCATT
    CCCCCAGCAG CCCCCCTTGC AGGGAGAAGG AGGCGACCCA CCAAAAGCAA AGGGAGCAAG
    TCCAGTCGCA GCAGCTCTTT GGGGAATAAA AGCCCTGGAC CAGGTACCCT GTCTGGCCAG
    AGTGCAGCTT CAGTCTTGCA CCCTCAGCAG ACCCTCCCCG CTCCTGGCAG CGTCCCGGAG
    ACAGGGCCCA ACCAGCTGTT ACAGCCTGTG AAGCCATCTC CTTCCAGTGA CAACCTGTAC
    TCGGCCTTTA CCAGCGATGG TGCCATTTCA GTACCAAGCC TTTCTGCGCC AGGTCAAGGC
    TGTGCCAAGT TTAACTGTGC GTCCGAGCAG GTGACGTTCA AAGCTGGCGG CAGGAGGACG
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCTTGC
    AACCAGCTCT GTAGGACCAG CAGCACCAAC GCCGTTGGGG GAGCCGTGAG CAGCCAGGCC
    GCCCCGGCTC CACCTCCTGC CGTGACGTCC AGCAGGAAGG GCATGTTCAC GGACGACCTG
    CACAAGCTGG TGGACAACTG GGCCCGGGAC GCCATGAGTC TCTCAGGCAG GCGGGGAAGC
    AAAGGACACG TGAGCTATGA GGGCCCTGGC ATGGCAAGGA AGTTCTCTGC GCCCGGTCAG
    CTGTGCGTCT CCATGACCTC AAACCTGGGC GGCTCTGCCC CCGGCTCTGC AGCGTCAGCT
    ACCTCTCTAG GGCACTTCCC CAAGTCCATG TGCCCCCCTC AGCAGTACGG TTTCCCTACC
    CCCCCGTTTG GCACGCAGTG GAGCGGGACG GGTGGCCCGG CACCCCAGCC GCTTAGCCAG
    TTCCAGCCTG TGGGAACAGC CTCCTTACAG AATTTCAACA TCAGCAACTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234923
    Clone ID Related Accession (Same CDS sequence) XM_024992724.1
    Accession Version XM_024992724.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGAG CACTCAGGTC
    GTCTCTCAGG TGGCTCCTCC AGAGCCAGCT CCCGGGGCTC AGCCACAGCC TGCCCAGCCA
    GCCGCGGTGG TCTCTTCTAT AGACAGCGCA CATTCAGATG TGGCTTCAGG CCTGAGTGAT
    GGCAACGAGA ATGTCCCCTC ATCCAGCGGG AGGCACGAGG GCAGGACCAC GAAGCGGCTG
    CATCGGAAGT CTATAAGGAG CCGCTCTCGT CACGAGAAGC CTTCCCGCCC CAAACTGAGA
    ATTCTCAATG TTTCAAATAA GGGAGATCGG GTGGTAGAAT GTCAGTTAGA GACTCACAAT
    CGGAAGATGG TTACATTCAA ATTTGATTTA GATGGTGACA ACCCTGAGGA GATAGCCACA
    ATTATGGTGA ACAATGACTT TATTCTGGCA CTGGAGAGAG ATGCGTTTGT GGAGCAAGTG
    CGGGAGATCA TTGAGAAAGC TGACGAGATG CTCAGTGAGG ATGTCAGCGT GGAGCCAGAG
    GGTGACCAGA GCTTGGAGAG TCTCCATGGC AAGGATGACT ATGGCTTCTC AGGCACCCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCATCTT CCTTGCCACA ACAGATAGGC
    ATTCCTGCCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTAAGT
    CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAACTTTTCA CTGAAATTTC AGATACGGTT
    GCTGCTTCCA CATCTGCTGG CCCTGGAATG AACTTGTCTC ACTCTGCCTC ATCCCTTAGT
    CTGCAGCAGG CCTTTTCTGA ACTTAGACAT GCTCGGATGG CAGAAGGGCC GAGTACGGCC
    CCTCCCCACT TTGGTCAGAC AGGCCCCGTG TTTCCAGCAG TGCCTCCCAC CCTGAGCAGT
    GCCCCTGGAG CCCCAGCCGC AGCAGCCGCA GCCTCGGTGT CAGCCCCTGC CCCAAGCCAC
    CCTCTTAGTG ACGTGACCAC ATCGGTCATT CAGACTGAGG TGACAGTGGC CCCTGGAACA
    GGTGTCATGC CTCCCAGTGG TCTCCCTGTA CCCCCTGTGT CTGAGTCCCT GGCACCTTCC
    AGCGTGGCCC CCAGCGTCAC CATCCCTGCA GTTCTCTCGG CACTACCAGC GCCCCAGGCG
    GCACAGGCTC CCACCCCTGG CAGCGGGGCC TCCAGTGCAG GCACTTTCCC CTCTGCACCC
    GCATCCCTGA CACCAGCCTC TGCTGTGGGC AGCGCTGTTG CCTCTGCTGC TCAGCTTCCG
    CCTGTGCCGT CTCAGCCCGC GGTAGGCAGC TCGACCGGGG TGGCCACGCT GACAGCCGTG
    CCCGCTGCCG CTCTGGGCCC TAGCCTCACG GCACTGCCGT CCCTTGCTCT CAGCAGCAGT
    ACCTCTGCCC CAGCTCTGGC AGAGACAGTG CTGGTCAGTG CCCACGCACT GGATAAGACG
    GCCCATAGTA GCACCACTGG CCTGGCTCTG TCCCTTCCTG CCACCTCGTC CTCTCCCGCC
    CCTGGAGCAG GAGTGTCCAG TGCTGTTTCT CAGCCTACTG CTGCGGCTCA CCCTTTGGTC
    CTCCCATCAG TCGTAACCTC TACTCCCGTA CTCTCCCAGG CAGGACCCAT GTCAGCACCT
    CTACTGCCCC AGGTACCCGG TATTCCACCC CTGGTACAAC CTGTTGTCAG CGTGCCTGCT
    GTGCAACAGA CCCTGGTTCA TAGTCAGCCT CAGCCCGCTC CACTCCCCAA CCAGCCCCAC
    ACTCACTGTC CCGAGATAGA CGCCGACACT CAACCCAAAG CTCCCGGGAT CGACGACATC
    AAGACTTTGG AGGAAAAGCT GCGATCTCTC TTTAGTGAGC ACAGTTCTTC TGGAGCCCAA
    CACGCCCCCG TCTCTCTGGA GACCTCGCTC ACTGTAGAGA CCGCTGTCAC AGCAGGCCTC
    CCAACCACTG CTGTTGCACC AAGCAAGCCT CTGACTTCCA CTGCCAGTAC TTGCTTGCCA
    CCAACCAGTC TGCCCTTAGG AGCAGCTGGC TTGTCAGTTA TACCAATGGT CACTCCTGGT
    CAAGTTTCCA CCCCAGTCAG CTCTGCGCCA GGAGTGAAAC TTGAAACTGC TCCAGCAAAG
    CCACCTTTAA CTAAGCCTCC AGTGCTGCCC ATGGGCACTG AACTTCCAGC TGGTACTCCA
    CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCACTAA CTCAGCCCCC GCAGCCCCTG
    GGAGACCTGG ACGCTCAGCT GAGAAGAACC CTCAGTCCCG AGAGTGGCAT GCTGACGTCT
    GCCGTTGGAC CCGTGTCTGT GGTGGCTCCA ACAGCAGTTG TGGAGACCGA AGCCCAACTT
    CCAAAGGATG TTCTTTCAGT CTCTCAAGTC ACAGAAGCTC CTGTCCTTGC GCCCACTTCC
    ACAGCTGGTG TTCTGAAGAT GGGGCGATTT CAGGTCTCAG TTGCAATGGA TGACCCCCAG
    AAGGAGGGTA AAAGTAAGTC GGAGGATGCC AAGTCTGTCC ACTTCGCATC CAGCACGTCA
    GAGTCCTCGG TGCTGTCAAG CAGCAGCCCC GAGAGCACCG TGGTGAAAGC AGAGCCCAAC
    GGGCTGGCCG TCCGGGGCGC CTCTGCAGAC ATGCCTGACA GCGCCCACCA GCCACCTGCC
    TCAGAAGCTG GGCAGCCTAG CAAGGTGGGC CGTTTTCAGG TGACCACCAC AGCGAGCAGA
    GTGGGTCGCT TCTCTGTGTC CAGAACTGAA GACAAGATGG CTGAGGTGAA GGGAGAGGAG
    CCAGTGGTCT CTCCTTTCAT GGACTCAGAA CACGACATTC CTCCCACCGT GACGCCAAAG
    AAAGAGAAAC CCGAACTGTC TGAGCCTTCG CATCTGAACG GGCCGTCTTC CGACCTGGAG
    GCTGCCTTCC TCAGCCGGGA CGTGGATGCT GGCTCAGGGA GCCCTCACTC CCCTCCGCAG
    CTGAGCTCCA AGAGCCTTCC TGCCCAGAGC CTCAGTCAGA GCCTCAGCAA TTCCTTCAAC
    TCTTCCTACA TGAGCAGCGA CAATGAGTCA GACATTGAAG ACGAGGACTT AAAGTTAGAG
    CTTCGCCGAC TACGGGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CACGAAATTG AGTCTTTATA CACCAAACTG GGCAAGGTTC CTCCTGCTGT CATCATTCCC
    CCAGCAGCCC CCCTTGCAGG GAGAAGGAGG CGACCCACCA AAAGCAAAGG GAGCAAGTCC
    AGTCGCAGCA GCTCTTTGGG GAATAAAAGC CCTGGACCAG GTACCCTGTC TGGCCAGAGT
    GCAGCTTCAG TCTTGCACCC TCAGCAGACC CTCCCCGCTC CTGGCAGCGT CCCGGAGACA
    GGGCCCAACC AGCTGTTACA GCCTGTGAAG CCATCTCCTT CCAGTGACAA CCTGTACTCG
    GCCTTTACCA GCGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCGCCAGG TCAAGGGACC
    AGCAGCACCA ACGCCGTTGG GGGAGCCGTG AGCAGCCAGG CCGCCCCGGC TCCACCTCCT
    GCCGTGACGT CCAGCAGGAA GGGCATGTTC ACGGACGACC TGCACAAGCT GGTGGACAAC
    TGGGCCCGGG ACGCCATGAG TCTCTCAGGC AGGCGGGGAA GCAAAGGACA CGTGAGCTAT
    GAGGGCCCTG GCATGGCAAG GAAGTTCTCT GCGCCCGGTC AGCTGTGCGT CTCCATGACC
    TCAAACCTGG GCGGCTCTGC CCCCGGCTCT GCAGCGTCAG CTACCTCTCT AGGGCACTTC
    CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC GGTTTCCCTA CCCCCCCGTT TGGCACGCAG
    TGGAGCGGGA CGGGTGGCCC GGCACCCCAG CCGCTTAGCC AGTTCCAGCC TGTGGGAACA
    GCCTCCTTAC AGAATTTCAA CATCAGCAAC TTGCAGAAAT CCATCAGCAA CCCCCCAGGT
    TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848492.1
    CDS130..6990
    Translation

    Target ORF information:

    RefSeq Version XM_024992724.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992724.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTCT CCAAGGCGAA CCTCAGATCC CAGTTGCAAC ACAGCCCTCA
    GTCGTTCCCG TCCATGCTGG TGCTCACTTC CTTCCTGTGG GACAGCCACT CCCACCCTCT
    TTACTGCCCC AGTACCCCGT CTCTCAGCTG CCCCCCATCT CTCAGCTCCC CTCTGCTCCT
    CCTCACGTGT CTGTGGCTCA GCCAGGCTTC CCACCCCTGC CGGTCACGAT GGCAGCTGGC
    GTTAGTCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTGCGG CGGCGGCCGT CCCCGGGGCA
    CCCACTGTGG TCCCTAGTCA GCTTCCTGCC CTCCTGCAGC CTGTGACCCA GCTGCCGAGT
    CAGGCTCACC CGCAGCTCCT GCAGCCAGCA GTTCAGTCCA TGGGGATCCC CGCCAGCCTT
    GGACAGACTG CCGAGGTGCC ACTTCCCCCT GGAGATATTC TCTACCAGAG CACTCAGGTC
    GTCTCTCAGG TGGCTCCTCC AGAGCCAGCT CCCGGGGCTC AGCCACAGCC TGCCCAGCCA
    GCCGCGGTGG TCTCTTCTAT AGACAGCGCA CATTCAGATG TGGCTTCAGG CCTGAGTGAT
    GGCAACGAGA ATGTCCCCTC ATCCAGCGGG AGGCACGAGG GCAGGACCAC GAAGCGGCTG
    CATCGGAAGT CTATAAGGAG CCGCTCTCGT CACGAGAAGC CTTCCCGCCC CAAACTGAGA
    ATTCTCAATG TTTCAAATAA GGGAGATCGG GTGGTAGAAT GTCAGTTAGA GACTCACAAT
    CGGAAGATGG TTACATTCAA ATTTGATTTA GATGGTGACA ACCCTGAGGA GATAGCCACA
    ATTATGGTGA ACAATGACTT TATTCTGGCA CTGGAGAGAG ATGCGTTTGT GGAGCAAGTG
    CGGGAGATCA TTGAGAAAGC TGACGAGATG CTCAGTGAGG ATGTCAGCGT GGAGCCAGAG
    GGTGACCAGA GCTTGGAGAG TCTCCATGGC AAGGATGACT ATGGCTTCTC AGGCACCCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCATCTT CCTTGCCACA ACAGATAGGC
    ATTCCTGCCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTAAGT
    CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAACTTTTCA CTGAAATTTC AGATACGGTT
    GCTGCTTCCA CATCTGCTGG CCCTGGAATG AACTTGTCTC ACTCTGCCTC ATCCCTTAGT
    CTGCAGCAGG CCTTTTCTGA ACTTAGACAT GCTCGGATGG CAGAAGGGCC GAGTACGGCC
    CCTCCCCACT TTGGTCAGAC AGGCCCCGTG TTTCCAGCAG TGCCTCCCAC CCTGAGCAGT
    GCCCCTGGAG CCCCAGCCGC AGCAGCCGCA GCCTCGGTGT CAGCCCCTGC CCCAAGCCAC
    CCTCTTAGTG ACGTGACCAC ATCGGTCATT CAGACTGAGG TGACAGTGGC CCCTGGAACA
    GGTGTCATGC CTCCCAGTGG TCTCCCTGTA CCCCCTGTGT CTGAGTCCCT GGCACCTTCC
    AGCGTGGCCC CCAGCGTCAC CATCCCTGCA GTTCTCTCGG CACTACCAGC GCCCCAGGCG
    GCACAGGCTC CCACCCCTGG CAGCGGGGCC TCCAGTGCAG GCACTTTCCC CTCTGCACCC
    GCATCCCTGA CACCAGCCTC TGCTGTGGGC AGCGCTGTTG CCTCTGCTGC TCAGCTTCCG
    CCTGTGCCGT CTCAGCCCGC GGTAGGCAGC TCGACCGGGG TGGCCACGCT GACAGCCGTG
    CCCGCTGCCG CTCTGGGCCC TAGCCTCACG GCACTGCCGT CCCTTGCTCT CAGCAGCAGT
    ACCTCTGCCC CAGCTCTGGC AGAGACAGTG CTGGTCAGTG CCCACGCACT GGATAAGACG
    GCCCATAGTA GCACCACTGG CCTGGCTCTG TCCCTTCCTG CCACCTCGTC CTCTCCCGCC
    CCTGGAGCAG GAGTGTCCAG TGCTGTTTCT CAGCCTACTG CTGCGGCTCA CCCTTTGGTC
    CTCCCATCAG TCGTAACCTC TACTCCCGTA CTCTCCCAGG CAGGACCCAT GTCAGCACCT
    CTACTGCCCC AGGTACCCGG TATTCCACCC CTGGTACAAC CTGTTGTCAG CGTGCCTGCT
    GTGCAACAGA CCCTGGTTCA TAGTCAGCCT CAGCCCGCTC CACTCCCCAA CCAGCCCCAC
    ACTCACTGTC CCGAGATAGA CGCCGACACT CAACCCAAAG CTCCCGGGAT CGACGACATC
    AAGACTTTGG AGGAAAAGCT GCGATCTCTC TTTAGTGAGC ACAGTTCTTC TGGAGCCCAA
    CACGCCCCCG TCTCTCTGGA GACCTCGCTC ACTGTAGAGA CCGCTGTCAC AGCAGGCCTC
    CCAACCACTG CTGTTGCACC AAGCAAGCCT CTGACTTCCA CTGCCAGTAC TTGCTTGCCA
    CCAACCAGTC TGCCCTTAGG AGCAGCTGGC TTGTCAGTTA TACCAATGGT CACTCCTGGT
    CAAGTTTCCA CCCCAGTCAG CTCTGCGCCA GGAGTGAAAC TTGAAACTGC TCCAGCAAAG
    CCACCTTTAA CTAAGCCTCC AGTGCTGCCC ATGGGCACTG AACTTCCAGC TGGTACTCCA
    CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCACTAA CTCAGCCCCC GCAGCCCCTG
    GGAGACCTGG ACGCTCAGCT GAGAAGAACC CTCAGTCCCG AGAGTGGCAT GCTGACGTCT
    GCCGTTGGAC CCGTGTCTGT GGTGGCTCCA ACAGCAGTTG TGGAGACCGA AGCCCAACTT
    CCAAAGGATG TTCTTTCAGT CTCTCAAGTC ACAGAAGCTC CTGTCCTTGC GCCCACTTCC
    ACAGCTGGTG TTCTGAAGAT GGGGCGATTT CAGGTCTCAG TTGCAATGGA TGACCCCCAG
    AAGGAGGGTA AAAGTAAGTC GGAGGATGCC AAGTCTGTCC ACTTCGCATC CAGCACGTCA
    GAGTCCTCGG TGCTGTCAAG CAGCAGCCCC GAGAGCACCG TGGTGAAAGC AGAGCCCAAC
    GGGCTGGCCG TCCGGGGCGC CTCTGCAGAC ATGCCTGACA GCGCCCACCA GCCACCTGCC
    TCAGAAGCTG GGCAGCCTAG CAAGGTGGGC CGTTTTCAGG TGACCACCAC AGCGAGCAGA
    GTGGGTCGCT TCTCTGTGTC CAGAACTGAA GACAAGATGG CTGAGGTGAA GGGAGAGGAG
    CCAGTGGTCT CTCCTTTCAT GGACTCAGAA CACGACATTC CTCCCACCGT GACGCCAAAG
    AAAGAGAAAC CCGAACTGTC TGAGCCTTCG CATCTGAACG GGCCGTCTTC CGACCTGGAG
    GCTGCCTTCC TCAGCCGGGA CGTGGATGCT GGCTCAGGGA GCCCTCACTC CCCTCCGCAG
    CTGAGCTCCA AGAGCCTTCC TGCCCAGAGC CTCAGTCAGA GCCTCAGCAA TTCCTTCAAC
    TCTTCCTACA TGAGCAGCGA CAATGAGTCA GACATTGAAG ACGAGGACTT AAAGTTAGAG
    CTTCGCCGAC TACGGGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CACGAAATTG AGTCTTTATA CACCAAACTG GGCAAGGTTC CTCCTGCTGT CATCATTCCC
    CCAGCAGCCC CCCTTGCAGG GAGAAGGAGG CGACCCACCA AAAGCAAAGG GAGCAAGTCC
    AGTCGCAGCA GCTCTTTGGG GAATAAAAGC CCTGGACCAG GTACCCTGTC TGGCCAGAGT
    GCAGCTTCAG TCTTGCACCC TCAGCAGACC CTCCCCGCTC CTGGCAGCGT CCCGGAGACA
    GGGCCCAACC AGCTGTTACA GCCTGTGAAG CCATCTCCTT CCAGTGACAA CCTGTACTCG
    GCCTTTACCA GCGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCGCCAGG TCAAGGGACC
    AGCAGCACCA ACGCCGTTGG GGGAGCCGTG AGCAGCCAGG CCGCCCCGGC TCCACCTCCT
    GCCGTGACGT CCAGCAGGAA GGGCATGTTC ACGGACGACC TGCACAAGCT GGTGGACAAC
    TGGGCCCGGG ACGCCATGAG TCTCTCAGGC AGGCGGGGAA GCAAAGGACA CGTGAGCTAT
    GAGGGCCCTG GCATGGCAAG GAAGTTCTCT GCGCCCGGTC AGCTGTGCGT CTCCATGACC
    TCAAACCTGG GCGGCTCTGC CCCCGGCTCT GCAGCGTCAG CTACCTCTCT AGGGCACTTC
    CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC GGTTTCCCTA CCCCCCCGTT TGGCACGCAG
    TGGAGCGGGA CGGGTGGCCC GGCACCCCAG CCGCTTAGCC AGTTCCAGCC TGTGGGAACA
    GCCTCCTTAC AGAATTTCAA CATCAGCAAC TTGCAGAAAT CCATCAGCAA CCCCCCAGGT
    TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234924
    Clone ID Related Accession (Same CDS sequence) XM_024992725.1
    Accession Version XM_024992725.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GGGCTTCCCA
    CCTCGACTGC CATCGCAGTA CCCAGGAGAC TCAAACATTG CCCCCTCCTC CAGCGCAGCT
    TCTGTGTGCC TGCCTTCTGC AGTCCTGTCC CCTCCCATGC CGACAGAGGC GCTGGCTGCC
    CCGGGTTACT TTCCTGCAAT GGTGCAGCCC TATGTGGAAT CCAGTCTCTT GGTTCCTGTG
    GGCAGTGTAG GAGGACAGGT CCAGGTGTCC CAGCCGGCAG TGAGCTTAGC ACAAGCCCCC
    ACCACATCCT CCCAGCAAGC AGCTCTGGAG AGCACTCAGG TCGTCTCTCA GGTGGCTCCT
    CCAGAGCCAG CTCCCGGGGC TCAGCCACAG CCTGCCCAGC CAGCCGCGGT GGTCTCTTCT
    ATAGACAGCG CACATTCAGA TGTGGCTTCA GGCCTGAGTG ATGGCAACGA GAATGTCCCC
    TCATCCAGCG GGAGGCACGA GGGCAGGACC ACGAAGCGGC TGCATCGGAA GTCTATAAGG
    AGCCGCTCTC GTCACGAGAA GCCTTCCCGC CCCAAACTGA GAATTCTCAA TGTTTCAAAT
    AAGGGAGATC GGGTGGTAGA ATGTCAGTTA GAGACTCACA ATCGGAAGAT GGTTACATTC
    AAATTTGATT TAGATGGTGA CAACCCTGAG GAGATAGCCA CAATTATGGT GAACAATGAC
    TTTATTCTGG CACTGGAGAG AGATGCGTTT GTGGAGCAAG TGCGGGAGAT CATTGAGAAA
    GCTGACGAGA TGCTCAGTGA GGATGTCAGC GTGGAGCCAG AGGGTGACCA GAGCTTGGAG
    AGTCTCCATG GCAAGGATGA CTATGGCTTC TCAGGCACCC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCATC TTCCTTGCCA CAACAGATAG CAGGCATTCC TGCCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TAAGTCCTGT GCCAGAAAGT
    CGATTACGAG AATCAAAACT TTTCACTGAA ATTTCAGATA CGGTTGCTGC TTCCACATCT
    GCTGGCCCTG GAATGAACTT GTCTCACTCT GCCTCATCCC TTAGTCTGCA GCAGGCCTTT
    TCTGAACTTA GACATGCTCG GATGGCAGAA GGGCCGAGTA CGGCCCCTCC CCACTTTGGT
    CAGACAGGCC CCGTGTTTCC AGCAGTGCCT CCCACCCTGA GCAGTGCCCC TGGAGCCCCA
    GCCGCAGCAG CCGCAGCCTC GGTGTCAGCC CCTGCCCCAA GCCACCCTCT TAGTGACGTG
    ACCACATCGG TCATTCAGAC TGAGGTGACA GTGGCCCCTG GAACAGGTGT CATGCCTCCC
    AGTGGTCTCC CTGTACCCCC TGTGTCTGAG TCCCTGGCAC CTTCCAGCGT GGCCCCCAGC
    GTCACCATCC CTGCAGTTCT CTCGGCACTA CCAGCGCCCC AGGCGGCACA GGCTCCCACC
    CCTGGCAGCG GGGCCTCCAG TGCAGGCACT TTCCCCTCTG CACCCGCATC CCTGACACCA
    GCCTCTGCTG TGGGCAGCGC TGTTGCCTCT GCTGCTCAGC TTCCGCCTGT GCCGTCTCAG
    CCCGCGGTAG GCAGCTCGAC CGGGGTGGCC ACGCTGACAG CCGTGCCCGC TGCCGCTCTG
    GGCCCTAGCC TCACGGCACT GCCGTCCCTT GCTCTCAGCA GCAGTACCTC TGCCCCAGCT
    CTGGCAGAGA CAGTGCTGGT CAGTGCCCAC GCACTGGATA AGACGGCCCA TAGTAGCACC
    ACTGGCCTGG CTCTGTCCCT TCCTGCCACC TCGTCCTCTC CCGCCCCTGG AGCAGGAGTG
    TCCAGTGCTG TTTCTCAGCC TACTGCTGCG GCTCACCCTT TGGTCCTCCC ATCAGTCGTA
    ACCTCTACTC CCGTACTCTC CCAGGCAGGA CCCATGTCAG CACCTCTACT GCCCCAGGTA
    CCCGGTATTC CACCCCTGGT ACAACCTGTT GTCAGCGTGC CTGCTGTGCA ACAGACCCTG
    GTTCATAGTC AGCCTCAGCC CGCTCCACTC CCCAACCAGC CCCACACTCA CTGTCCCGAG
    ATAGACGCCG ACACTCAACC CAAAGCTCCC GGGATCGACG ACATCAAGAC TTTGGAGGAA
    AAGCTGCGAT CTCTCTTTAG TGAGCACAGT TCTTCTGGAG CCCAACACGC CCCCGTCTCT
    CTGGAGACCT CGCTCACTGT AGAGACCGCT GTCACAGCAG GCCTCCCAAC CACTGCTGTT
    GCACCAAGCA AGCCTCTGAC TTCCACTGCC AGTACTTGCT TGCCACCAAC CAGTCTGCCC
    TTAGGAGCAG CTGGCTTGTC AGTTATACCA ATGGTCACTC CTGGTCAAGT TTCCACCCCA
    GTCAGCTCTG CGCCAGGAGT GAAACTTGAA ACTGCTCCAG CAAAGCCACC TTTAACTAAG
    CCTCCAGTGC TGCCCATGGG CACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG
    CCACCTTTTC CAGGACCTTC ACTAACTCAG CCCCCGCAGC CCCTGGGAGA CCTGGACGCT
    CAGCTGAGAA GAACCCTCAG TCCCGAGAGT GGCATGCTGA CGTCTGCCGT TGGACCCGTG
    TCTGTGGTGG CTCCAACAGC AGTTGTGGAG ACCGAAGCCC AACTTCCAAA GGATGTTCTT
    TCAGTCTCTC AAGTCACAGA AGCTCCTGTC CTTGCGCCCA CTTCCACAGC TGGTGTTCTG
    AAGATGGGGC GATTTCAGGT CTCAGTTGCA ATGGATGACC CCCAGAAGGA GGGTAAAAGT
    AAGTCGGAGG ATGCCAAGTC TGTCCACTTC GCATCCAGCA CGTCAGAGTC CTCGGTGCTG
    TCAAGCAGCA GCCCCGAGAG CACCGTGGTG AAAGCAGAGC CCAACGGGCT GGCCGTCCGG
    GGCGCCTCTG CAGACATGCC TGACAGCGCC CACCAGCCAC CTGCCTCAGA AGCTGGGCAG
    CCTAGCAAGG TGGGCCGTTT TCAGGTGACC ACCACAGCGA GCAGAGTGGG TCGCTTCTCT
    GTGTCCAGAA CTGAAGACAA GATGGCTGAG GTGAAGGGAG AGGAGCCAGT GGTCTCTCCT
    TTCATGGACT CAGAACACGA CATTCCTCCC ACCGTGACGC CAAAGAAAGA GAAACCCGAA
    CTGTCTGAGC CTTCGCATCT GAACGGGCCG TCTTCCGACC TGGAGGCTGC CTTCCTCAGC
    CGGGACGTGG ATGCTGGCTC AGGGAGCCCT CACTCCCCTC CGCAGCTGAG CTCCAAGAGC
    CTTCCTGCCC AGAGCCTCAG TCAGAGCCTC AGCAATTCCT TCAACTCTTC CTACATGAGC
    AGCGACAATG AGTCAGACAT TGAAGACGAG GACTTAAAGT TAGAGCTTCG CCGACTACGG
    GAAAAACATC TTAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCACGA AATTGAGTCT
    TTATACACCA AACTGGGCAA GGTTCCTCCT GCTGTCATCA TTCCCCCAGC AGCCCCCCTT
    GCAGGGAGAA GGAGGCGACC CACCAAAAGC AAAGGGAGCA AGTCCAGTCG CAGCAGCTCT
    TTGGGGAATA AAAGCCCTGG ACCAGGTACC CTGTCTGGCC AGAGTGCAGC TTCAGTCTTG
    CACCCTCAGC AGACCCTCCC CGCTCCTGGC AGCGTCCCGG AGACAGGGCC CAACCAGCTG
    TTACAGCCTG TGAAGCCATC TCCTTCCAGT GACAACCTGT ACTCGGCCTT TACCAGCGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCG CCAGGTCAAG GCTGTGCCAA GTTTAACTGT
    GCGTCCGAGC AGGTGACGTT CAAAGCTGGC GGCAGGAGGA CGCGATTTCT GAGTACGCCC
    TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA GTCTGCCCTT GCAACCAGCT CTGTAGGACC
    AGCAGCACCA ACGCCGTTGG GGGAGCCGTG AGCAGCCAGG CCGCCCCGGC TCCACCTCCT
    GCCGTGACGT CCAGCAGGAA GGGCATGTTC ACGGACGACC TGCACAAGCT GGTGGACAAC
    TGGGCCCGGG ACGCCATGAG TCTCTCAGGC AGGCGGGGAA GCAAAGGACA CGTGAGCTAT
    GAGGGCCCTG GCATGGCAAG GAAGTTCTCT GCGCCCGGTC AGCTGTGCGT CTCCATGACC
    TCAAACCTGG GCGGCTCTGC CCCCGGCTCT GCAGCGTCAG CTACCTCTCT AGGGCACTTC
    CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC GGTTTCCCTA CCCCCCCGTT TGGCACGCAG
    TGGAGCGGGA CGGGTGGCCC GGCACCCCAG CCGCTTAGCC AGTTCCAGCC TGTGGGAACA
    GCCTCCTTAC AGAATTTCAA CATCAGCAAC TTGCAGAAAT CCATCAGCAA CCCCCCAGGT
    TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848493.1
    CDS131..6931
    Translation

    Target ORF information:

    RefSeq Version XM_024992725.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GGGCTTCCCA
    CCTCGACTGC CATCGCAGTA CCCAGGAGAC TCAAACATTG CCCCCTCCTC CAGCGCAGCT
    TCTGTGTGCC TGCCTTCTGC AGTCCTGTCC CCTCCCATGC CGACAGAGGC GCTGGCTGCC
    CCGGGTTACT TTCCTGCAAT GGTGCAGCCC TATGTGGAAT CCAGTCTCTT GGTTCCTGTG
    GGCAGTGTAG GAGGACAGGT CCAGGTGTCC CAGCCGGCAG TGAGCTTAGC ACAAGCCCCC
    ACCACATCCT CCCAGCAAGC AGCTCTGGAG AGCACTCAGG TCGTCTCTCA GGTGGCTCCT
    CCAGAGCCAG CTCCCGGGGC TCAGCCACAG CCTGCCCAGC CAGCCGCGGT GGTCTCTTCT
    ATAGACAGCG CACATTCAGA TGTGGCTTCA GGCCTGAGTG ATGGCAACGA GAATGTCCCC
    TCATCCAGCG GGAGGCACGA GGGCAGGACC ACGAAGCGGC TGCATCGGAA GTCTATAAGG
    AGCCGCTCTC GTCACGAGAA GCCTTCCCGC CCCAAACTGA GAATTCTCAA TGTTTCAAAT
    AAGGGAGATC GGGTGGTAGA ATGTCAGTTA GAGACTCACA ATCGGAAGAT GGTTACATTC
    AAATTTGATT TAGATGGTGA CAACCCTGAG GAGATAGCCA CAATTATGGT GAACAATGAC
    TTTATTCTGG CACTGGAGAG AGATGCGTTT GTGGAGCAAG TGCGGGAGAT CATTGAGAAA
    GCTGACGAGA TGCTCAGTGA GGATGTCAGC GTGGAGCCAG AGGGTGACCA GAGCTTGGAG
    AGTCTCCATG GCAAGGATGA CTATGGCTTC TCAGGCACCC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCATC TTCCTTGCCA CAACAGATAG CAGGCATTCC TGCCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TAAGTCCTGT GCCAGAAAGT
    CGATTACGAG AATCAAAACT TTTCACTGAA ATTTCAGATA CGGTTGCTGC TTCCACATCT
    GCTGGCCCTG GAATGAACTT GTCTCACTCT GCCTCATCCC TTAGTCTGCA GCAGGCCTTT
    TCTGAACTTA GACATGCTCG GATGGCAGAA GGGCCGAGTA CGGCCCCTCC CCACTTTGGT
    CAGACAGGCC CCGTGTTTCC AGCAGTGCCT CCCACCCTGA GCAGTGCCCC TGGAGCCCCA
    GCCGCAGCAG CCGCAGCCTC GGTGTCAGCC CCTGCCCCAA GCCACCCTCT TAGTGACGTG
    ACCACATCGG TCATTCAGAC TGAGGTGACA GTGGCCCCTG GAACAGGTGT CATGCCTCCC
    AGTGGTCTCC CTGTACCCCC TGTGTCTGAG TCCCTGGCAC CTTCCAGCGT GGCCCCCAGC
    GTCACCATCC CTGCAGTTCT CTCGGCACTA CCAGCGCCCC AGGCGGCACA GGCTCCCACC
    CCTGGCAGCG GGGCCTCCAG TGCAGGCACT TTCCCCTCTG CACCCGCATC CCTGACACCA
    GCCTCTGCTG TGGGCAGCGC TGTTGCCTCT GCTGCTCAGC TTCCGCCTGT GCCGTCTCAG
    CCCGCGGTAG GCAGCTCGAC CGGGGTGGCC ACGCTGACAG CCGTGCCCGC TGCCGCTCTG
    GGCCCTAGCC TCACGGCACT GCCGTCCCTT GCTCTCAGCA GCAGTACCTC TGCCCCAGCT
    CTGGCAGAGA CAGTGCTGGT CAGTGCCCAC GCACTGGATA AGACGGCCCA TAGTAGCACC
    ACTGGCCTGG CTCTGTCCCT TCCTGCCACC TCGTCCTCTC CCGCCCCTGG AGCAGGAGTG
    TCCAGTGCTG TTTCTCAGCC TACTGCTGCG GCTCACCCTT TGGTCCTCCC ATCAGTCGTA
    ACCTCTACTC CCGTACTCTC CCAGGCAGGA CCCATGTCAG CACCTCTACT GCCCCAGGTA
    CCCGGTATTC CACCCCTGGT ACAACCTGTT GTCAGCGTGC CTGCTGTGCA ACAGACCCTG
    GTTCATAGTC AGCCTCAGCC CGCTCCACTC CCCAACCAGC CCCACACTCA CTGTCCCGAG
    ATAGACGCCG ACACTCAACC CAAAGCTCCC GGGATCGACG ACATCAAGAC TTTGGAGGAA
    AAGCTGCGAT CTCTCTTTAG TGAGCACAGT TCTTCTGGAG CCCAACACGC CCCCGTCTCT
    CTGGAGACCT CGCTCACTGT AGAGACCGCT GTCACAGCAG GCCTCCCAAC CACTGCTGTT
    GCACCAAGCA AGCCTCTGAC TTCCACTGCC AGTACTTGCT TGCCACCAAC CAGTCTGCCC
    TTAGGAGCAG CTGGCTTGTC AGTTATACCA ATGGTCACTC CTGGTCAAGT TTCCACCCCA
    GTCAGCTCTG CGCCAGGAGT GAAACTTGAA ACTGCTCCAG CAAAGCCACC TTTAACTAAG
    CCTCCAGTGC TGCCCATGGG CACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG
    CCACCTTTTC CAGGACCTTC ACTAACTCAG CCCCCGCAGC CCCTGGGAGA CCTGGACGCT
    CAGCTGAGAA GAACCCTCAG TCCCGAGAGT GGCATGCTGA CGTCTGCCGT TGGACCCGTG
    TCTGTGGTGG CTCCAACAGC AGTTGTGGAG ACCGAAGCCC AACTTCCAAA GGATGTTCTT
    TCAGTCTCTC AAGTCACAGA AGCTCCTGTC CTTGCGCCCA CTTCCACAGC TGGTGTTCTG
    AAGATGGGGC GATTTCAGGT CTCAGTTGCA ATGGATGACC CCCAGAAGGA GGGTAAAAGT
    AAGTCGGAGG ATGCCAAGTC TGTCCACTTC GCATCCAGCA CGTCAGAGTC CTCGGTGCTG
    TCAAGCAGCA GCCCCGAGAG CACCGTGGTG AAAGCAGAGC CCAACGGGCT GGCCGTCCGG
    GGCGCCTCTG CAGACATGCC TGACAGCGCC CACCAGCCAC CTGCCTCAGA AGCTGGGCAG
    CCTAGCAAGG TGGGCCGTTT TCAGGTGACC ACCACAGCGA GCAGAGTGGG TCGCTTCTCT
    GTGTCCAGAA CTGAAGACAA GATGGCTGAG GTGAAGGGAG AGGAGCCAGT GGTCTCTCCT
    TTCATGGACT CAGAACACGA CATTCCTCCC ACCGTGACGC CAAAGAAAGA GAAACCCGAA
    CTGTCTGAGC CTTCGCATCT GAACGGGCCG TCTTCCGACC TGGAGGCTGC CTTCCTCAGC
    CGGGACGTGG ATGCTGGCTC AGGGAGCCCT CACTCCCCTC CGCAGCTGAG CTCCAAGAGC
    CTTCCTGCCC AGAGCCTCAG TCAGAGCCTC AGCAATTCCT TCAACTCTTC CTACATGAGC
    AGCGACAATG AGTCAGACAT TGAAGACGAG GACTTAAAGT TAGAGCTTCG CCGACTACGG
    GAAAAACATC TTAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCACGA AATTGAGTCT
    TTATACACCA AACTGGGCAA GGTTCCTCCT GCTGTCATCA TTCCCCCAGC AGCCCCCCTT
    GCAGGGAGAA GGAGGCGACC CACCAAAAGC AAAGGGAGCA AGTCCAGTCG CAGCAGCTCT
    TTGGGGAATA AAAGCCCTGG ACCAGGTACC CTGTCTGGCC AGAGTGCAGC TTCAGTCTTG
    CACCCTCAGC AGACCCTCCC CGCTCCTGGC AGCGTCCCGG AGACAGGGCC CAACCAGCTG
    TTACAGCCTG TGAAGCCATC TCCTTCCAGT GACAACCTGT ACTCGGCCTT TACCAGCGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCG CCAGGTCAAG GCTGTGCCAA GTTTAACTGT
    GCGTCCGAGC AGGTGACGTT CAAAGCTGGC GGCAGGAGGA CGCGATTTCT GAGTACGCCC
    TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA GTCTGCCCTT GCAACCAGCT CTGTAGGACC
    AGCAGCACCA ACGCCGTTGG GGGAGCCGTG AGCAGCCAGG CCGCCCCGGC TCCACCTCCT
    GCCGTGACGT CCAGCAGGAA GGGCATGTTC ACGGACGACC TGCACAAGCT GGTGGACAAC
    TGGGCCCGGG ACGCCATGAG TCTCTCAGGC AGGCGGGGAA GCAAAGGACA CGTGAGCTAT
    GAGGGCCCTG GCATGGCAAG GAAGTTCTCT GCGCCCGGTC AGCTGTGCGT CTCCATGACC
    TCAAACCTGG GCGGCTCTGC CCCCGGCTCT GCAGCGTCAG CTACCTCTCT AGGGCACTTC
    CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC GGTTTCCCTA CCCCCCCGTT TGGCACGCAG
    TGGAGCGGGA CGGGTGGCCC GGCACCCCAG CCGCTTAGCC AGTTCCAGCC TGTGGGAACA
    GCCTCCTTAC AGAATTTCAA CATCAGCAAC TTGCAGAAAT CCATCAGCAA CCCCCCAGGT
    TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234925
    Clone ID Related Accession (Same CDS sequence) XM_024992727.1
    Accession Version XM_024992727.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GGGCTTCCCA
    CCTCGACTGC CATCGCAGTA CCCAGGAGAC TCAAACATTG CCCCCTCCTC CAGCGCAGCT
    TCTGTGTGCC TGCCTTCTGC AGTCCTGTCC CCTCCCATGC CGACAGAGGC GCTGGCTGCC
    CCGGGTTACT TTCCTGCAAT GGTGCAGCCC TATGTGGAAT CCAGTCTCTT GGTTCCTGTG
    GGCAGTGTAG GAGGACAGGT CCAGGTGTCC CAGCCGGCAG TGAGCTTAGC ACAAGCCCCC
    ACCACATCCT CCCAGCAAGC AGCTCTGGAG AGCACTCAGG TCGTCTCTCA GGTGGCTCCT
    CCAGAGCCAG CTCCCGGGGC TCAGCCACAG CCTGCCCAGC CAGCCGCGGT GGTCTCTTCT
    ATAGACAGCG CACATTCAGA TGTGGCTTCA GGCCTGAGTG ATGGCAACGA GAATGTCCCC
    TCATCCAGCG GGAGGCACGA GGGCAGGACC ACGAAGCGGC TGCATCGGAA GTCTATAAGG
    AGCCGCTCTC GTCACGAGAA GCCTTCCCGC CCCAAACTGA GAATTCTCAA TGTTTCAAAT
    AAGGGAGATC GGGTGGTAGA ATGTCAGTTA GAGACTCACA ATCGGAAGAT GGTTACATTC
    AAATTTGATT TAGATGGTGA CAACCCTGAG GAGATAGCCA CAATTATGGT GAACAATGAC
    TTTATTCTGG CACTGGAGAG AGATGCGTTT GTGGAGCAAG TGCGGGAGAT CATTGAGAAA
    GCTGACGAGA TGCTCAGTGA GGATGTCAGC GTGGAGCCAG AGGGTGACCA GAGCTTGGAG
    AGTCTCCATG GCAAGGATGA CTATGGCTTC TCAGGCACCC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCATC TTCCTTGCCA CAACAGATAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGCT GTGCCAAGTT TAACTGTGCG
    TCCGAGCAGG TGACGTTCAA AGCTGGCGGC AGGAGGACGC GATTTCTGAG TACGCCCTGC
    TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGCCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACCAACG CCGTTGGGGG AGCCGTGAGC AGCCAGGCCG CCCCGGCTCC ACCTCCTGCC
    GTGACGTCCA GCAGGAAGGG CATGTTCACG GACGACCTGC ACAAGCTGGT GGACAACTGG
    GCCCGGGACG CCATGAGTCT CTCAGGCAGG CGGGGAAGCA AAGGACACGT GAGCTATGAG
    GGCCCTGGCA TGGCAAGGAA GTTCTCTGCG CCCGGTCAGC TGTGCGTCTC CATGACCTCA
    AACCTGGGCG GCTCTGCCCC CGGCTCTGCA GCGTCAGCTA CCTCTCTAGG GCACTTCCCC
    AAGTCCATGT GCCCCCCTCA GCAGTACGGT TTCCCTACCC CCCCGTTTGG CACGCAGTGG
    AGCGGGACGG GTGGCCCGGC ACCCCAGCCG CTTAGCCAGT TCCAGCCTGT GGGAACAGCC
    TCCTTACAGA ATTTCAACAT CAGCAACTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848495.1
    CDS130..6927
    Translation

    Target ORF information:

    RefSeq Version XM_024992727.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GGGCTTCCCA
    CCTCGACTGC CATCGCAGTA CCCAGGAGAC TCAAACATTG CCCCCTCCTC CAGCGCAGCT
    TCTGTGTGCC TGCCTTCTGC AGTCCTGTCC CCTCCCATGC CGACAGAGGC GCTGGCTGCC
    CCGGGTTACT TTCCTGCAAT GGTGCAGCCC TATGTGGAAT CCAGTCTCTT GGTTCCTGTG
    GGCAGTGTAG GAGGACAGGT CCAGGTGTCC CAGCCGGCAG TGAGCTTAGC ACAAGCCCCC
    ACCACATCCT CCCAGCAAGC AGCTCTGGAG AGCACTCAGG TCGTCTCTCA GGTGGCTCCT
    CCAGAGCCAG CTCCCGGGGC TCAGCCACAG CCTGCCCAGC CAGCCGCGGT GGTCTCTTCT
    ATAGACAGCG CACATTCAGA TGTGGCTTCA GGCCTGAGTG ATGGCAACGA GAATGTCCCC
    TCATCCAGCG GGAGGCACGA GGGCAGGACC ACGAAGCGGC TGCATCGGAA GTCTATAAGG
    AGCCGCTCTC GTCACGAGAA GCCTTCCCGC CCCAAACTGA GAATTCTCAA TGTTTCAAAT
    AAGGGAGATC GGGTGGTAGA ATGTCAGTTA GAGACTCACA ATCGGAAGAT GGTTACATTC
    AAATTTGATT TAGATGGTGA CAACCCTGAG GAGATAGCCA CAATTATGGT GAACAATGAC
    TTTATTCTGG CACTGGAGAG AGATGCGTTT GTGGAGCAAG TGCGGGAGAT CATTGAGAAA
    GCTGACGAGA TGCTCAGTGA GGATGTCAGC GTGGAGCCAG AGGGTGACCA GAGCTTGGAG
    AGTCTCCATG GCAAGGATGA CTATGGCTTC TCAGGCACCC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCATC TTCCTTGCCA CAACAGATAG GCATTCCTGC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTAA GTCCTGTGCC AGAAAGTCGA
    TTACGAGAAT CAAAACTTTT CACTGAAATT TCAGATACGG TTGCTGCTTC CACATCTGCT
    GGCCCTGGAA TGAACTTGTC TCACTCTGCC TCATCCCTTA GTCTGCAGCA GGCCTTTTCT
    GAACTTAGAC ATGCTCGGAT GGCAGAAGGG CCGAGTACGG CCCCTCCCCA CTTTGGTCAG
    ACAGGCCCCG TGTTTCCAGC AGTGCCTCCC ACCCTGAGCA GTGCCCCTGG AGCCCCAGCC
    GCAGCAGCCG CAGCCTCGGT GTCAGCCCCT GCCCCAAGCC ACCCTCTTAG TGACGTGACC
    ACATCGGTCA TTCAGACTGA GGTGACAGTG GCCCCTGGAA CAGGTGTCAT GCCTCCCAGT
    GGTCTCCCTG TACCCCCTGT GTCTGAGTCC CTGGCACCTT CCAGCGTGGC CCCCAGCGTC
    ACCATCCCTG CAGTTCTCTC GGCACTACCA GCGCCCCAGG CGGCACAGGC TCCCACCCCT
    GGCAGCGGGG CCTCCAGTGC AGGCACTTTC CCCTCTGCAC CCGCATCCCT GACACCAGCC
    TCTGCTGTGG GCAGCGCTGT TGCCTCTGCT GCTCAGCTTC CGCCTGTGCC GTCTCAGCCC
    GCGGTAGGCA GCTCGACCGG GGTGGCCACG CTGACAGCCG TGCCCGCTGC CGCTCTGGGC
    CCTAGCCTCA CGGCACTGCC GTCCCTTGCT CTCAGCAGCA GTACCTCTGC CCCAGCTCTG
    GCAGAGACAG TGCTGGTCAG TGCCCACGCA CTGGATAAGA CGGCCCATAG TAGCACCACT
    GGCCTGGCTC TGTCCCTTCC TGCCACCTCG TCCTCTCCCG CCCCTGGAGC AGGAGTGTCC
    AGTGCTGTTT CTCAGCCTAC TGCTGCGGCT CACCCTTTGG TCCTCCCATC AGTCGTAACC
    TCTACTCCCG TACTCTCCCA GGCAGGACCC ATGTCAGCAC CTCTACTGCC CCAGGTACCC
    GGTATTCCAC CCCTGGTACA ACCTGTTGTC AGCGTGCCTG CTGTGCAACA GACCCTGGTT
    CATAGTCAGC CTCAGCCCGC TCCACTCCCC AACCAGCCCC ACACTCACTG TCCCGAGATA
    GACGCCGACA CTCAACCCAA AGCTCCCGGG ATCGACGACA TCAAGACTTT GGAGGAAAAG
    CTGCGATCTC TCTTTAGTGA GCACAGTTCT TCTGGAGCCC AACACGCCCC CGTCTCTCTG
    GAGACCTCGC TCACTGTAGA GACCGCTGTC ACAGCAGGCC TCCCAACCAC TGCTGTTGCA
    CCAAGCAAGC CTCTGACTTC CACTGCCAGT ACTTGCTTGC CACCAACCAG TCTGCCCTTA
    GGAGCAGCTG GCTTGTCAGT TATACCAATG GTCACTCCTG GTCAAGTTTC CACCCCAGTC
    AGCTCTGCGC CAGGAGTGAA ACTTGAAACT GCTCCAGCAA AGCCACCTTT AACTAAGCCT
    CCAGTGCTGC CCATGGGCAC TGAACTTCCA GCTGGTACTC CACCCAGCGA GCAGCTGCCA
    CCTTTTCCAG GACCTTCACT AACTCAGCCC CCGCAGCCCC TGGGAGACCT GGACGCTCAG
    CTGAGAAGAA CCCTCAGTCC CGAGAGTGGC ATGCTGACGT CTGCCGTTGG ACCCGTGTCT
    GTGGTGGCTC CAACAGCAGT TGTGGAGACC GAAGCCCAAC TTCCAAAGGA TGTTCTTTCA
    GTCTCTCAAG TCACAGAAGC TCCTGTCCTT GCGCCCACTT CCACAGCTGG TGTTCTGAAG
    ATGGGGCGAT TTCAGGTCTC AGTTGCAATG GATGACCCCC AGAAGGAGGG TAAAAGTAAG
    TCGGAGGATG CCAAGTCTGT CCACTTCGCA TCCAGCACGT CAGAGTCCTC GGTGCTGTCA
    AGCAGCAGCC CCGAGAGCAC CGTGGTGAAA GCAGAGCCCA ACGGGCTGGC CGTCCGGGGC
    GCCTCTGCAG ACATGCCTGA CAGCGCCCAC CAGCCACCTG CCTCAGAAGC TGGGCAGCCT
    AGCAAGGTGG GCCGTTTTCA GGTGACCACC ACAGCGAGCA GAGTGGGTCG CTTCTCTGTG
    TCCAGAACTG AAGACAAGAT GGCTGAGGTG AAGGGAGAGG AGCCAGTGGT CTCTCCTTTC
    ATGGACTCAG AACACGACAT TCCTCCCACC GTGACGCCAA AGAAAGAGAA ACCCGAACTG
    TCTGAGCCTT CGCATCTGAA CGGGCCGTCT TCCGACCTGG AGGCTGCCTT CCTCAGCCGG
    GACGTGGATG CTGGCTCAGG GAGCCCTCAC TCCCCTCCGC AGCTGAGCTC CAAGAGCCTT
    CCTGCCCAGA GCCTCAGTCA GAGCCTCAGC AATTCCTTCA ACTCTTCCTA CATGAGCAGC
    GACAATGAGT CAGACATTGA AGACGAGGAC TTAAAGTTAG AGCTTCGCCG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCACGAAAT TGAGTCTTTA
    TACACCAAAC TGGGCAAGGT TCCTCCTGCT GTCATCATTC CCCCAGCAGC CCCCCTTGCA
    GGGAGAAGGA GGCGACCCAC CAAAAGCAAA GGGAGCAAGT CCAGTCGCAG CAGCTCTTTG
    GGGAATAAAA GCCCTGGACC AGGTACCCTG TCTGGCCAGA GTGCAGCTTC AGTCTTGCAC
    CCTCAGCAGA CCCTCCCCGC TCCTGGCAGC GTCCCGGAGA CAGGGCCCAA CCAGCTGTTA
    CAGCCTGTGA AGCCATCTCC TTCCAGTGAC AACCTGTACT CGGCCTTTAC CAGCGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCGCCA GGTCAAGGCT GTGCCAAGTT TAACTGTGCG
    TCCGAGCAGG TGACGTTCAA AGCTGGCGGC AGGAGGACGC GATTTCTGAG TACGCCCTGC
    TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGCCCTTGCA ACCAGCTCTG TAGGACCAGC
    AGCACCAACG CCGTTGGGGG AGCCGTGAGC AGCCAGGCCG CCCCGGCTCC ACCTCCTGCC
    GTGACGTCCA GCAGGAAGGG CATGTTCACG GACGACCTGC ACAAGCTGGT GGACAACTGG
    GCCCGGGACG CCATGAGTCT CTCAGGCAGG CGGGGAAGCA AAGGACACGT GAGCTATGAG
    GGCCCTGGCA TGGCAAGGAA GTTCTCTGCG CCCGGTCAGC TGTGCGTCTC CATGACCTCA
    AACCTGGGCG GCTCTGCCCC CGGCTCTGCA GCGTCAGCTA CCTCTCTAGG GCACTTCCCC
    AAGTCCATGT GCCCCCCTCA GCAGTACGGT TTCCCTACCC CCCCGTTTGG CACGCAGTGG
    AGCGGGACGG GTGGCCCGGC ACCCCAGCCG CTTAGCCAGT TCCAGCCTGT GGGAACAGCC
    TCCTTACAGA ATTTCAACAT CAGCAACTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC
    AACCTGCGGA CCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234926
    Clone ID Related Accession (Same CDS sequence) XM_024992728.1
    Accession Version XM_024992728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGGACTCT GCGACTGGTT TAGGCCAGTC GTGATTCCTT TCCTTGGACT GGAGGTAGGA 
    CCTGCTTCTC TTCTGTCGCA AGGTGGATGC TTGAACCAAG ATCGAAAGTT AACAAAGTCT
    GAGAGGCAGC GATTTAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAGCA TCCCAATATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAGGGAA AGAAGTGCAT TGTGTTGGTG
    ACTGAACTGA TGACATCTGG AACTCTTAAA ACGTACCTGA AAAGGTTTAA GGTGATGAAG
    ATCAAAGTTT TGAGAAGCTG GTGCCGTCAG ATTCTTAAAG GACTTCAGTT TCTTCATACT
    AGAACTCCAC CTATTATTCA CCGGGATCTT AAGTGTGACA ACATCTTTAT CACTGGCCCC
    ACAGGCTCAG TCAAGATTGG AGACCTAGGT CTGGCCACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAAGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTCG AGATGGCCAC GTCTGAATAC
    CCGTACTCAG AGTGTCAGAA CGCTGCACAG ATCTACCGTC GTGTGACCAG CGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGGCAAAACA AAGATGAAAG ATACTCTATC AAAGACCTTT TGAACCATGC CTTCTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCCATT
    AAATTATGGC TACGTATTGA AGATATTAAG AAACTAAAGG GGAAATACAA AGACAATGAA
    GCTATCGAGT TTTCCTTTGA CTTGGAGAAA GATGTGCCAG AAGACGTTGC TCAAGAAATG
    GTAGATTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCAAAAGC CATCAAAGAT
    AGAGTGTCGT TAATTAAGAG AAAACGAGAA CAGCGTCAAT TAGTGCGGGA GGAGCAAGAA
    AAAAGAAAGC AGGAGGAGAG CAGCTTCAAG CAGCAGGTGG AGCAGCAGCC CAGCACTGCC
    CAGGCCGGAG TCCAGCAGCC CCCTGCCACC AGCACTGGTG GACCCGCTGC CTCTGCTGCG
    TCTGCGTCCG TCTCCACGCA GGTAGAGCCA GAAGAGCCTG AGGCCGACCA GCACCAGCAG
    CTGCAGTACC AGCAGCCTGG TGTGTCTGTG ATATCTGACG GGACGGTTGA CAGTGGTCAG
    GGGTCTTCTG TCTTCACGGA ATCTCGAGTC AGCAGCCAAC AGACAGTTTC GTACGGGTCC
    CAGCATGAGC AGGCACCTTC CACTGGCGCC CTCCCAGGGC ACACAGCATC TGTTGTCCAA
    ACGCAGGCTC AGCCCCATGC GGTATATCCA CCCTCAAGTT TGGCACAGGG GCAGAGCCAG
    GGTCAGCCAT CTTCAAGTAG TTTAACAGGG GTTCCATCCT CCCAACCCAT GCAGCATTCT
    CAGCAGCAGC AAGGAGTGCA GCCAACAGCC CCCTCTCAGC AGGCGGTGCA GTATCCGCTC
    CCTCAGACAT CGGCCCCCAG CGAGGCCACG ATTGCACAGC CTGTGAGTCA GCCTCCACCT
    CCACAGGCCT TGCCACACGT GTCCGCGGGA AAACAGCTTC CAGTTTCCCA GCCAGTACCA
    ACTCTCCAAG GCGAACCTCA GATCCCAGTT GCAACACAGC CCTCAGTCGT TCCCGTCCAT
    GCTGGTGCTC ACTTCCTTCC TGTGGGACAG CCACTCCCAC CCTCTTTACT GCCCCAGTAC
    CCCGTCTCTC AGCTGCCCCC CATCTCTCAG CTCCCCTCTG CTCCTCCTCA CGTGTCTGTG
    GCTCAGCCAG GCTTCCCACC CCTGCCGGTC ACGATGGCAG CTGGCGTTAG TCAGCCTCTG
    CTCACCTTGG CTTCATCTGC TGCGGCGGCG GCCGTCCCCG GGGCACCCAC TGTGGTCCCT
    AGTCAGCTTC CTGCCCTCCT GCAGCCTGTG ACCCAGCTGC CGAGTCAGGC TCACCCGCAG
    CTCCTGCAGC CAGCAGTTCA GTCCATGGGG ATCCCCGCCA GCCTTGGACA GACTGCCGAG
    GTGCCACTTC CCCCTGGAGA TATTCTCTAC CAGGGCTTCC CACCTCGACT GCCATCGCAG
    TACCCAGGAG ACTCAAACAT TGCCCCCTCC TCCAGCGCAG CTTCTGTGTG CCTGCCTTCT
    GCAGTCCTGT CCCCTCCCAT GCCGACAGAG GCGCTGGCTG CCCCGGGTTA CTTTCCTGCA
    ATGGTGCAGC CCTATGTGGA ATCCAGTCTC TTGGTTCCTG TGGGCAGTGT AGGAGGACAG
    GTCCAGGTGT CCCAGCCGGC AGTGAGCTTA GCACAAGCCC CCACCACATC CTCCCAGCAA
    GCAGCTCTGG AGAGCACTCA GGTCGTCTCT CAGGTGGCTC CTCCAGAGCC AGCTCCCGGG
    GCTCAGCCAC AGCCTGCCCA GCCAGCCGCG GTGGTCTCTT CTATAGACAG CGCACATTCA
    GATGTGGCTT CAGGCCTGAG TGATGGCAAC GAGAATGTCC CCTCATCCAG CGGGAGGCAC
    GAGGGCAGGA CCACGAAGCG GCTGCATCGG AAGTCTATAA GGAGCCGCTC TCGTCACGAG
    AAGCCTTCCC GCCCCAAACT GAGAATTCTC AATGTTTCAA ATAAGGGAGA TCGGGTGGTA
    GAATGTCAGT TAGAGACTCA CAATCGGAAG ATGGTTACAT TCAAATTTGA TTTAGATGGT
    GACAACCCTG AGGAGATAGC CACAATTATG GTGAACAATG ACTTTATTCT GGCACTGGAG
    AGAGATGCGT TTGTGGAGCA AGTGCGGGAG ATCATTGAGA AAGCTGACGA GATGCTCAGT
    GAGGATGTCA GCGTGGAGCC AGAGGGTGAC CAGAGCTTGG AGAGTCTCCA TGGCAAGGAT
    GACTATGGCT TCTCAGGCAC CCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCA
    TCTTCCTTGC CACAACAGAT AGCAGGCATT CCTGCCAGTT CTTTAACTCA AGTTGTTCAT
    TCTGCTGGAA GACGGTTTAT AGTAAGTCCT GTGCCAGAAA GTCGATTACG AGAATCAAAA
    CTTTTCACTG AAATTTCAGA TACGGTTGCT GCTTCCACAT CTGCTGGCCC TGGAATGAAC
    TTGTCTCACT CTGCCTCATC CCTTAGTCTG CAGCAGGCCT TTTCTGAACT TAGACATGCT
    CGGATGGCAG AAGGGCCGAG TACGGCCCCT CCCCACTTTG GTCAGACAGG CCCCGTGTTT
    CCAGCAGTGC CTCCCACCCT GAGCAGTGCC CCTGGAGCCC CAGCCGCAGC AGCCGCAGCC
    TCGGTGTCAG CCCCTGCCCC AAGCCACCCT CTTAGTGACG TGACCACATC GGTCATTCAG
    ACTGAGGTGA CAGTGGCCCC TGGAACAGGT GTCATGCCTC CCAGTGGTCT CCCTGTACCC
    CCTGTGTCTG AGTCCCTGGC ACCTTCCAGC GTGGCCCCCA GCGTCACCAT CCCTGCAGTT
    CTCTCGGCAC TACCAGCGCC CCAGGCGGCA CAGGCTCCCA CCCCTGGCAG CGGGGCCTCC
    AGTGCAGGCA CTTTCCCCTC TGCACCCGCA TCCCTGACAC CAGCCTCTGC TGTGGGCAGC
    GCTGTTGCCT CTGCTGCTCA GCTTCCGCCT GTGCCGTCTC AGCCCGCGGT AGGCAGCTCG
    ACCGGGGTGG CCACGCTGAC AGCCGTGCCC GCTGCCGCTC TGGGCCCTAG CCTCACGGCA
    CTGCCGTCCC TTGCTCTCAG CAGCAGTACC TCTGCCCCAG CTCTGGCAGA GACAGTGCTG
    GTCAGTGCCC ACGCACTGGA TAAGACGGCC CATAGTAGCA CCACTGGCCT GGCTCTGTCC
    CTTCCTGCCA CCTCGTCCTC TCCCGCCCCT GGAGCAGGAG TGTCCAGTGC TGTTTCTCAG
    CCTACTGCTG CGGCTCACCC TTTGGTCCTC CCATCAGTCG TAACCTCTAC TCCCGTACTC
    TCCCAGGCAG GACCCATGTC AGCACCTCTA CTGCCCCAGG TACCCGGTAT TCCACCCCTG
    GTACAACCTG TTGTCAGCGT GCCTGCTGTG CAACAGACCC TGGTTCATAG TCAGCCTCAG
    CCCGCTCCAC TCCCCAACCA GCCCCACACT CACTGTCCCG AGATAGACGC CGACACTCAA
    CCCAAAGCTC CCGGGATCGA CGACATCAAG ACTTTGGAGG AAAAGCTGCG ATCTCTCTTT
    AGTGAGCACA GTTCTTCTGG AGCCCAACAC GCCCCCGTCT CTCTGGAGAC CTCGCTCACT
    GTAGAGACCG CTGTCACAGC AGGCCTCCCA ACCACTGCTG TTGCACCAAG CAAGCCTCTG
    ACTTCCACTG CCAGTACTTG CTTGCCACCA ACCAGTCTGC CCTTAGGAGC AGCTGGCTTG
    TCAGTTATAC CAATGGTCAC TCCTGGTCAA GTTTCCACCC CAGTCAGCTC TGCGCCAGGA
    GTGAAACTTG AAACTGCTCC AGCAAAGCCA CCTTTAACTA AGCCTCCAGT GCTGCCCATG
    GGCACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT
    TCACTAACTC AGCCCCCGCA GCCCCTGGGA GACCTGGACG CTCAGCTGAG AAGAACCCTC
    AGTCCCGAGA GTGGCATGCT GACGTCTGCC GTTGGACCCG TGTCTGTGGT GGCTCCAACA
    GCAGTTGTGG AGACCGAAGC CCAACTTCCA AAGGATGTTC TTTCAGTCTC TCAAGTCACA
    GAAGCTCCTG TCCTTGCGCC CACTTCCACA GCTGGTGTTC TGAAGATGGG GCGATTTCAG
    GTCTCAGTTG CAATGGATGA CCCCCAGAAG GAGGGTAAAA GTAAGTCGGA GGATGCCAAG
    TCTGTCCACT TCGCATCCAG CACGTCAGAG TCCTCGGTGC TGTCAAGCAG CAGCCCCGAG
    AGCACCGTGG TGAAAGCAGA GCCCAACGGG CTGGCCGTCC GGGGCGCCTC TGCAGACATG
    CCTGACAGCG CCCACCAGCC ACCTGCCTCA GAAGCTGGGC AGCCTAGCAA GGTGGGCCGT
    TTTCAGGTGA CCACCACAGC GAGCAGAGTG GGTCGCTTCT CTGTGTCCAG AACTGAAGAC
    AAGATGGCTG AGGTGAAGGG AGAGGAGCCA GTGGTCTCTC CTTTCATGGA CTCAGAACAC
    GACATTCCTC CCACCGTGAC GCCAAAGAAA GAGAAACCCG AACTGTCTGA GCCTTCGCAT
    CTGAACGGGC CGTCTTCCGA CCTGGAGGCT GCCTTCCTCA GCCGGGACGT GGATGCTGGC
    TCAGGGAGCC CTCACTCCCC TCCGCAGCTG AGCTCCAAGA GCCTTCCTGC CCAGAGCCTC
    AGTCAGAGCC TCAGCAATTC CTTCAACTCT TCCTACATGA GCAGCGACAA TGAGTCAGAC
    ATTGAAGACG AGGACTTAAA GTTAGAGCTT CGCCGACTAC GGGAAAAACA TCTTAAAGAG
    ATTCAGGACC TGCAGAGTCG CCAGAAGCAC GAAATTGAGT CTTTATACAC CAAACTGGGC
    AAGGTTCCTC CTGCTGTCAT CATTCCCCCA GCAGCCCCCC TTGCAGGGAG AAGGAGGCGA
    CCCACCAAAA GCAAAGGGAG CAAGTCCAGT CGCAGCAGCT CTTTGGGGAA TAAAAGCCCT
    GGACCAGGTA CCCTGTCTGG CCAGAGTGCA GCTTCAGTCT TGCACCCTCA GCAGACCCTC
    CCCGCTCCTG GCAGCGTCCC GGAGACAGGG CCCAACCAGC TGTTACAGCC TGTGAAGCCA
    TCTCCTTCCA GTGACAACCT GTACTCGGCC TTTACCAGCG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CGCCAGGTCA AGGCTGTGCC AAGTTTAACT GTGCGTCCGA GCAGGTGACG
    TTCAAAGCTG GCGGCAGGAG GACGCGATTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG
    ATGGTGAAAA AAGTCTGCCC TTGCAACCAG CTCTGTAGGA CCAGCAGCAC CAACGCCGTT
    GGGGGAGCCG TGAGCAGCCA GGCCGCCCCG GCTCCACCTC CTGCCGTGAC GTCCAGCAGG
    AAGGGCATGT TCACGGACGA CCTGCACAAG CTGGTGGACA ACTGGGCCCG GGACGCCATG
    AGTCTCTCAG GCAGGCGGGG AAGCAAAGGA CACGTGAGCT ATGAGGGCCC TGGCATGGCA
    AGGAAGTTCT CTGCGCCCGG TCAGCTGTGC GTCTCCATGA CCTCAAACCT GGGCGGCTCT
    GCCCCCGGCT CTGCAGCGTC AGCTACCTCT CTAGGGCACT TCCCCAAGTC CATGTGCCCC
    CCTCAGCAGT ACGGTTTCCC TACCCCCCCG TTTGGCACGC AGTGGAGCGG GACGGGTGGC
    CCGGCACCCC AGCCGCTTAG CCAGTTCCAG CCTGTGGGAA CAGCCTCCTT ACAGAATTTC
    AACATCAGCA ACTTGCAGAA ATCCATCAGC AACCCCCCAG GTTCCAACCT GCGGACCACG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848496.1
    CDS16..6618
    Translation

    Target ORF information:

    RefSeq Version XM_024992728.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGGACTCT GCGACTGGTT TAGGCCAGTC GTGATTCCTT TCCTTGGACT GGAGGTAGGA 
    CCTGCTTCTC TTCTGTCGCA AGGTGGATGC TTGAACCAAG ATCGAAAGTT AACAAAGTCT
    GAGAGGCAGC GATTTAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAGCA TCCCAATATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAGGGAA AGAAGTGCAT TGTGTTGGTG
    ACTGAACTGA TGACATCTGG AACTCTTAAA ACGTACCTGA AAAGGTTTAA GGTGATGAAG
    ATCAAAGTTT TGAGAAGCTG GTGCCGTCAG ATTCTTAAAG GACTTCAGTT TCTTCATACT
    AGAACTCCAC CTATTATTCA CCGGGATCTT AAGTGTGACA ACATCTTTAT CACTGGCCCC
    ACAGGCTCAG TCAAGATTGG AGACCTAGGT CTGGCCACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAAGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTCG AGATGGCCAC GTCTGAATAC
    CCGTACTCAG AGTGTCAGAA CGCTGCACAG ATCTACCGTC GTGTGACCAG CGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGGCAAAACA AAGATGAAAG ATACTCTATC AAAGACCTTT TGAACCATGC CTTCTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCCATT
    AAATTATGGC TACGTATTGA AGATATTAAG AAACTAAAGG GGAAATACAA AGACAATGAA
    GCTATCGAGT TTTCCTTTGA CTTGGAGAAA GATGTGCCAG AAGACGTTGC TCAAGAAATG
    GTAGATTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCAAAAGC CATCAAAGAT
    AGAGTGTCGT TAATTAAGAG AAAACGAGAA CAGCGTCAAT TAGTGCGGGA GGAGCAAGAA
    AAAAGAAAGC AGGAGGAGAG CAGCTTCAAG CAGCAGGTGG AGCAGCAGCC CAGCACTGCC
    CAGGCCGGAG TCCAGCAGCC CCCTGCCACC AGCACTGGTG GACCCGCTGC CTCTGCTGCG
    TCTGCGTCCG TCTCCACGCA GGTAGAGCCA GAAGAGCCTG AGGCCGACCA GCACCAGCAG
    CTGCAGTACC AGCAGCCTGG TGTGTCTGTG ATATCTGACG GGACGGTTGA CAGTGGTCAG
    GGGTCTTCTG TCTTCACGGA ATCTCGAGTC AGCAGCCAAC AGACAGTTTC GTACGGGTCC
    CAGCATGAGC AGGCACCTTC CACTGGCGCC CTCCCAGGGC ACACAGCATC TGTTGTCCAA
    ACGCAGGCTC AGCCCCATGC GGTATATCCA CCCTCAAGTT TGGCACAGGG GCAGAGCCAG
    GGTCAGCCAT CTTCAAGTAG TTTAACAGGG GTTCCATCCT CCCAACCCAT GCAGCATTCT
    CAGCAGCAGC AAGGAGTGCA GCCAACAGCC CCCTCTCAGC AGGCGGTGCA GTATCCGCTC
    CCTCAGACAT CGGCCCCCAG CGAGGCCACG ATTGCACAGC CTGTGAGTCA GCCTCCACCT
    CCACAGGCCT TGCCACACGT GTCCGCGGGA AAACAGCTTC CAGTTTCCCA GCCAGTACCA
    ACTCTCCAAG GCGAACCTCA GATCCCAGTT GCAACACAGC CCTCAGTCGT TCCCGTCCAT
    GCTGGTGCTC ACTTCCTTCC TGTGGGACAG CCACTCCCAC CCTCTTTACT GCCCCAGTAC
    CCCGTCTCTC AGCTGCCCCC CATCTCTCAG CTCCCCTCTG CTCCTCCTCA CGTGTCTGTG
    GCTCAGCCAG GCTTCCCACC CCTGCCGGTC ACGATGGCAG CTGGCGTTAG TCAGCCTCTG
    CTCACCTTGG CTTCATCTGC TGCGGCGGCG GCCGTCCCCG GGGCACCCAC TGTGGTCCCT
    AGTCAGCTTC CTGCCCTCCT GCAGCCTGTG ACCCAGCTGC CGAGTCAGGC TCACCCGCAG
    CTCCTGCAGC CAGCAGTTCA GTCCATGGGG ATCCCCGCCA GCCTTGGACA GACTGCCGAG
    GTGCCACTTC CCCCTGGAGA TATTCTCTAC CAGGGCTTCC CACCTCGACT GCCATCGCAG
    TACCCAGGAG ACTCAAACAT TGCCCCCTCC TCCAGCGCAG CTTCTGTGTG CCTGCCTTCT
    GCAGTCCTGT CCCCTCCCAT GCCGACAGAG GCGCTGGCTG CCCCGGGTTA CTTTCCTGCA
    ATGGTGCAGC CCTATGTGGA ATCCAGTCTC TTGGTTCCTG TGGGCAGTGT AGGAGGACAG
    GTCCAGGTGT CCCAGCCGGC AGTGAGCTTA GCACAAGCCC CCACCACATC CTCCCAGCAA
    GCAGCTCTGG AGAGCACTCA GGTCGTCTCT CAGGTGGCTC CTCCAGAGCC AGCTCCCGGG
    GCTCAGCCAC AGCCTGCCCA GCCAGCCGCG GTGGTCTCTT CTATAGACAG CGCACATTCA
    GATGTGGCTT CAGGCCTGAG TGATGGCAAC GAGAATGTCC CCTCATCCAG CGGGAGGCAC
    GAGGGCAGGA CCACGAAGCG GCTGCATCGG AAGTCTATAA GGAGCCGCTC TCGTCACGAG
    AAGCCTTCCC GCCCCAAACT GAGAATTCTC AATGTTTCAA ATAAGGGAGA TCGGGTGGTA
    GAATGTCAGT TAGAGACTCA CAATCGGAAG ATGGTTACAT TCAAATTTGA TTTAGATGGT
    GACAACCCTG AGGAGATAGC CACAATTATG GTGAACAATG ACTTTATTCT GGCACTGGAG
    AGAGATGCGT TTGTGGAGCA AGTGCGGGAG ATCATTGAGA AAGCTGACGA GATGCTCAGT
    GAGGATGTCA GCGTGGAGCC AGAGGGTGAC CAGAGCTTGG AGAGTCTCCA TGGCAAGGAT
    GACTATGGCT TCTCAGGCAC CCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCA
    TCTTCCTTGC CACAACAGAT AGCAGGCATT CCTGCCAGTT CTTTAACTCA AGTTGTTCAT
    TCTGCTGGAA GACGGTTTAT AGTAAGTCCT GTGCCAGAAA GTCGATTACG AGAATCAAAA
    CTTTTCACTG AAATTTCAGA TACGGTTGCT GCTTCCACAT CTGCTGGCCC TGGAATGAAC
    TTGTCTCACT CTGCCTCATC CCTTAGTCTG CAGCAGGCCT TTTCTGAACT TAGACATGCT
    CGGATGGCAG AAGGGCCGAG TACGGCCCCT CCCCACTTTG GTCAGACAGG CCCCGTGTTT
    CCAGCAGTGC CTCCCACCCT GAGCAGTGCC CCTGGAGCCC CAGCCGCAGC AGCCGCAGCC
    TCGGTGTCAG CCCCTGCCCC AAGCCACCCT CTTAGTGACG TGACCACATC GGTCATTCAG
    ACTGAGGTGA CAGTGGCCCC TGGAACAGGT GTCATGCCTC CCAGTGGTCT CCCTGTACCC
    CCTGTGTCTG AGTCCCTGGC ACCTTCCAGC GTGGCCCCCA GCGTCACCAT CCCTGCAGTT
    CTCTCGGCAC TACCAGCGCC CCAGGCGGCA CAGGCTCCCA CCCCTGGCAG CGGGGCCTCC
    AGTGCAGGCA CTTTCCCCTC TGCACCCGCA TCCCTGACAC CAGCCTCTGC TGTGGGCAGC
    GCTGTTGCCT CTGCTGCTCA GCTTCCGCCT GTGCCGTCTC AGCCCGCGGT AGGCAGCTCG
    ACCGGGGTGG CCACGCTGAC AGCCGTGCCC GCTGCCGCTC TGGGCCCTAG CCTCACGGCA
    CTGCCGTCCC TTGCTCTCAG CAGCAGTACC TCTGCCCCAG CTCTGGCAGA GACAGTGCTG
    GTCAGTGCCC ACGCACTGGA TAAGACGGCC CATAGTAGCA CCACTGGCCT GGCTCTGTCC
    CTTCCTGCCA CCTCGTCCTC TCCCGCCCCT GGAGCAGGAG TGTCCAGTGC TGTTTCTCAG
    CCTACTGCTG CGGCTCACCC TTTGGTCCTC CCATCAGTCG TAACCTCTAC TCCCGTACTC
    TCCCAGGCAG GACCCATGTC AGCACCTCTA CTGCCCCAGG TACCCGGTAT TCCACCCCTG
    GTACAACCTG TTGTCAGCGT GCCTGCTGTG CAACAGACCC TGGTTCATAG TCAGCCTCAG
    CCCGCTCCAC TCCCCAACCA GCCCCACACT CACTGTCCCG AGATAGACGC CGACACTCAA
    CCCAAAGCTC CCGGGATCGA CGACATCAAG ACTTTGGAGG AAAAGCTGCG ATCTCTCTTT
    AGTGAGCACA GTTCTTCTGG AGCCCAACAC GCCCCCGTCT CTCTGGAGAC CTCGCTCACT
    GTAGAGACCG CTGTCACAGC AGGCCTCCCA ACCACTGCTG TTGCACCAAG CAAGCCTCTG
    ACTTCCACTG CCAGTACTTG CTTGCCACCA ACCAGTCTGC CCTTAGGAGC AGCTGGCTTG
    TCAGTTATAC CAATGGTCAC TCCTGGTCAA GTTTCCACCC CAGTCAGCTC TGCGCCAGGA
    GTGAAACTTG AAACTGCTCC AGCAAAGCCA CCTTTAACTA AGCCTCCAGT GCTGCCCATG
    GGCACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT
    TCACTAACTC AGCCCCCGCA GCCCCTGGGA GACCTGGACG CTCAGCTGAG AAGAACCCTC
    AGTCCCGAGA GTGGCATGCT GACGTCTGCC GTTGGACCCG TGTCTGTGGT GGCTCCAACA
    GCAGTTGTGG AGACCGAAGC CCAACTTCCA AAGGATGTTC TTTCAGTCTC TCAAGTCACA
    GAAGCTCCTG TCCTTGCGCC CACTTCCACA GCTGGTGTTC TGAAGATGGG GCGATTTCAG
    GTCTCAGTTG CAATGGATGA CCCCCAGAAG GAGGGTAAAA GTAAGTCGGA GGATGCCAAG
    TCTGTCCACT TCGCATCCAG CACGTCAGAG TCCTCGGTGC TGTCAAGCAG CAGCCCCGAG
    AGCACCGTGG TGAAAGCAGA GCCCAACGGG CTGGCCGTCC GGGGCGCCTC TGCAGACATG
    CCTGACAGCG CCCACCAGCC ACCTGCCTCA GAAGCTGGGC AGCCTAGCAA GGTGGGCCGT
    TTTCAGGTGA CCACCACAGC GAGCAGAGTG GGTCGCTTCT CTGTGTCCAG AACTGAAGAC
    AAGATGGCTG AGGTGAAGGG AGAGGAGCCA GTGGTCTCTC CTTTCATGGA CTCAGAACAC
    GACATTCCTC CCACCGTGAC GCCAAAGAAA GAGAAACCCG AACTGTCTGA GCCTTCGCAT
    CTGAACGGGC CGTCTTCCGA CCTGGAGGCT GCCTTCCTCA GCCGGGACGT GGATGCTGGC
    TCAGGGAGCC CTCACTCCCC TCCGCAGCTG AGCTCCAAGA GCCTTCCTGC CCAGAGCCTC
    AGTCAGAGCC TCAGCAATTC CTTCAACTCT TCCTACATGA GCAGCGACAA TGAGTCAGAC
    ATTGAAGACG AGGACTTAAA GTTAGAGCTT CGCCGACTAC GGGAAAAACA TCTTAAAGAG
    ATTCAGGACC TGCAGAGTCG CCAGAAGCAC GAAATTGAGT CTTTATACAC CAAACTGGGC
    AAGGTTCCTC CTGCTGTCAT CATTCCCCCA GCAGCCCCCC TTGCAGGGAG AAGGAGGCGA
    CCCACCAAAA GCAAAGGGAG CAAGTCCAGT CGCAGCAGCT CTTTGGGGAA TAAAAGCCCT
    GGACCAGGTA CCCTGTCTGG CCAGAGTGCA GCTTCAGTCT TGCACCCTCA GCAGACCCTC
    CCCGCTCCTG GCAGCGTCCC GGAGACAGGG CCCAACCAGC TGTTACAGCC TGTGAAGCCA
    TCTCCTTCCA GTGACAACCT GTACTCGGCC TTTACCAGCG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CGCCAGGTCA AGGCTGTGCC AAGTTTAACT GTGCGTCCGA GCAGGTGACG
    TTCAAAGCTG GCGGCAGGAG GACGCGATTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG
    ATGGTGAAAA AAGTCTGCCC TTGCAACCAG CTCTGTAGGA CCAGCAGCAC CAACGCCGTT
    GGGGGAGCCG TGAGCAGCCA GGCCGCCCCG GCTCCACCTC CTGCCGTGAC GTCCAGCAGG
    AAGGGCATGT TCACGGACGA CCTGCACAAG CTGGTGGACA ACTGGGCCCG GGACGCCATG
    AGTCTCTCAG GCAGGCGGGG AAGCAAAGGA CACGTGAGCT ATGAGGGCCC TGGCATGGCA
    AGGAAGTTCT CTGCGCCCGG TCAGCTGTGC GTCTCCATGA CCTCAAACCT GGGCGGCTCT
    GCCCCCGGCT CTGCAGCGTC AGCTACCTCT CTAGGGCACT TCCCCAAGTC CATGTGCCCC
    CCTCAGCAGT ACGGTTTCCC TACCCCCCCG TTTGGCACGC AGTGGAGCGG GACGGGTGGC
    CCGGCACCCC AGCCGCTTAG CCAGTTCCAG CCTGTGGGAA CAGCCTCCTT ACAGAATTTC
    AACATCAGCA ACTTGCAGAA ATCCATCAGC AACCCCCCAG GTTCCAACCT GCGGACCACG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234927
    Clone ID Related Accession (Same CDS sequence) XM_024992729.1
    Accession Version XM_024992729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GAGCACTCAG
    GTCGTCTCTC AGGTGGCTCC TCCAGAGCCA GCTCCCGGGG CTCAGCCACA GCCTGCCCAG
    CCAGCCGCGG TGGTCTCTTC TATAGACAGC GCACATTCAG ATGTGGCTTC AGGCCTGAGT
    GATGGCAACG AGAATGTCCC CTCATCCAGC GGGAGGCACG AGGGCAGGAC CACGAAGCGG
    CTGCATCGGA AGTCTATAAG GAGCCGCTCT CGTCACGAGA AGCCTTCCCG CCCCAAACTG
    AGAATTCTCA ATGTTTCAAA TAAGGGAGAT CGGGTGGTAG AATGTCAGTT AGAGACTCAC
    AATCGGAAGA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAACCCTGA GGAGATAGCC
    ACAATTATGG TGAACAATGA CTTTATTCTG GCACTGGAGA GAGATGCGTT TGTGGAGCAA
    GTGCGGGAGA TCATTGAGAA AGCTGACGAG ATGCTCAGTG AGGATGTCAG CGTGGAGCCA
    GAGGGTGACC AGAGCTTGGA GAGTCTCCAT GGCAAGGATG ACTATGGCTT CTCAGGCACC
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCAT CTTCCTTGCC ACAACAGATA
    GCAGGCATTC CTGCCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTAAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAC TTTTCACTGA AATTTCAGAT
    ACGGTTGCTG CTTCCACATC TGCTGGCCCT GGAATGAACT TGTCTCACTC TGCCTCATCC
    CTTAGTCTGC AGCAGGCCTT TTCTGAACTT AGACATGCTC GGATGGCAGA AGGGCCGAGT
    ACGGCCCCTC CCCACTTTGG TCAGACAGGC CCCGTGTTTC CAGCAGTGCC TCCCACCCTG
    AGCAGTGCCC CTGGAGCCCC AGCCGCAGCA GCCGCAGCCT CGGTGTCAGC CCCTGCCCCA
    AGCCACCCTC TTAGTGACGT GACCACATCG GTCATTCAGA CTGAGGTGAC AGTGGCCCCT
    GGAACAGGTG TCATGCCTCC CAGTGGTCTC CCTGTACCCC CTGTGTCTGA GTCCCTGGCA
    CCTTCCAGCG TGGCCCCCAG CGTCACCATC CCTGCAGTTC TCTCGGCACT ACCAGCGCCC
    CAGGCGGCAC AGGCTCCCAC CCCTGGCAGC GGGGCCTCCA GTGCAGGCAC TTTCCCCTCT
    GCACCCGCAT CCCTGACACC AGCCTCTGCT GTGGGCAGCG CTGTTGCCTC TGCTGCTCAG
    CTTCCGCCTG TGCCGTCTCA GCCCGCGGTA GGCAGCTCGA CCGGGGTGGC CACGCTGACA
    GCCGTGCCCG CTGCCGCTCT GGGCCCTAGC CTCACGGCAC TGCCGTCCCT TGCTCTCAGC
    AGCAGTACCT CTGCCCCAGC TCTGGCAGAG ACAGTGCTGG TCAGTGCCCA CGCACTGGAT
    AAGACGGCCC ATAGTAGCAC CACTGGCCTG GCTCTGTCCC TTCCTGCCAC CTCGTCCTCT
    CCCGCCCCTG GAGCAGGAGT GTCCAGTGCT GTTTCTCAGC CTACTGCTGC GGCTCACCCT
    TTGGTCCTCC CATCAGTCGT AACCTCTACT CCCGTACTCT CCCAGGCAGG ACCCATGTCA
    GCACCTCTAC TGCCCCAGGT ACCCGGTATT CCACCCCTGG TACAACCTGT TGTCAGCGTG
    CCTGCTGTGC AACAGACCCT GGTTCATAGT CAGCCTCAGC CCGCTCCACT CCCCAACCAG
    CCCCACACTC ACTGTCCCGA GATAGACGCC GACACTCAAC CCAAAGCTCC CGGGATCGAC
    GACATCAAGA CTTTGGAGGA AAAGCTGCGA TCTCTCTTTA GTGAGCACAG TTCTTCTGGA
    GCCCAACACG CCCCCGTCTC TCTGGAGACC TCGCTCACTG TAGAGACCGC TGTCACAGCA
    GGCCTCCCAA CCACTGCTGT TGCACCAAGC AAGCCTCTGA CTTCCACTGC CAGTACTTGC
    TTGCCACCAA CCAGTCTGCC CTTAGGAGCA GCTGGCTTGT CAGTTATACC AATGGTCACT
    CCTGGTCAAG TTTCCACCCC AGTCAGCTCT GCGCCAGGAG TGAAACTTGA AACTGCTCCA
    GCAAAGCCAC CTTTAACTAA GCCTCCAGTG CTGCCCATGG GCACTGAACT TCCAGCTGGT
    ACTCCACCCA GCGAGCAGCT GCCACCTTTT CCAGGACCTT CACTAACTCA GCCCCCGCAG
    CCCCTGGGAG ACCTGGACGC TCAGCTGAGA AGAACCCTCA GTCCCGAGAG TGGCATGCTG
    ACGTCTGCCG TTGGACCCGT GTCTGTGGTG GCTCCAACAG CAGTTGTGGA GACCGAAGCC
    CAACTTCCAA AGGATGTTCT TTCAGTCTCT CAAGTCACAG AAGCTCCTGT CCTTGCGCCC
    ACTTCCACAG CTGGTGTTCT GAAGATGGGG CGATTTCAGG TCTCAGTTGC AATGGATGAC
    CCCCAGAAGG AGGGTAAAAG TAAGTCGGAG GATGCCAAGT CTGTCCACTT CGCATCCAGC
    ACGTCAGAGT CCTCGGTGCT GTCAAGCAGC AGCCCCGAGA GCACCGTGGT GAAAGCAGAG
    CCCAACGGGC TGGCCGTCCG GGGCGCCTCT GCAGACATGC CTGACAGCGC CCACCAGCCA
    CCTGCCTCAG AAGCTGGGCA GCCTAGCAAG GTGGGCCGTT TTCAGGTGAC CACCACAGCG
    AGCAGAGTGG GTCGCTTCTC TGTGTCCAGA ACTGAAGACA AGATGGCTGA GGTGAAGGGA
    GAGGAGCCAG TGGTCTCTCC TTTCATGGAC TCAGAACACG ACATTCCTCC CACCGTGACG
    CCAAAGAAAG AGAAACCCGA ACTGTCTGAG CCTTCGCATC TGAACGGGCC GTCTTCCGAC
    CTGGAGGCTG CCTTCCTCAG CCGGGACGTG GATGCTGGCT CAGGGAGCCC TCACTCCCCT
    CCGCAGCTGA GCTCCAAGAG CCTTCCTGCC CAGAGCCTCA GTCAGAGCCT CAGCAATTCC
    TTCAACTCTT CCTACATGAG CAGCGACAAT GAGTCAGACA TTGAAGACGA GGACTTAAAG
    TTAGAGCTTC GCCGACTACG GGAAAAACAT CTTAAAGAGA TTCAGGACCT GCAGAGTCGC
    CAGAAGCACG AAATTGAGTC TTTATACACC AAACTGGGCA AGGTTCCTCC TGCTGTCATC
    ATTCCCCCAG CAGCCCCCCT TGCAGGGAGA AGGAGGCGAC CCACCAAAAG CAAAGGGAGC
    AAGTCCAGTC GCAGCAGCTC TTTGGGGAAT AAAAGCCCTG GACCAGGTAC CCTGTCTGGC
    CAGAGTGCAG CTTCAGTCTT GCACCCTCAG CAGACCCTCC CCGCTCCTGG CAGCGTCCCG
    GAGACAGGGC CCAACCAGCT GTTACAGCCT GTGAAGCCAT CTCCTTCCAG TGACAACCTG
    TACTCGGCCT TTACCAGCGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC GCCAGGTCAA
    GGCTGTGCCA AGTTTAACTG TGCGTCCGAG CAGGTGACGT TCAAAGCTGG CGGCAGGAGG
    ACGCGATTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGCCCT
    TGCAACCAGC TCTGTAGGAC CAGCAGCACC AACGCCGTTG GGGGAGCCGT GAGCAGCCAG
    GCCGCCCCGG CTCCACCTCC TGCCGTGACG TCCAGCAGGA AGGGCATGTT CACGGACGAC
    CTGCACAAGC TGGTGGACAA CTGGGCCCGG GACGCCATGA GTCTCTCAGG CAGGCGGGGA
    AGCAAAGGAC ACGTGAGCTA TGAGGGCCCT GGCATGGCAA GGAAGTTCTC TGCGCCCGGT
    CAGCTGTGCG TCTCCATGAC CTCAAACCTG GGCGGCTCTG CCCCCGGCTC TGCAGCGTCA
    GCTACCTCTC TAGGGCACTT CCCCAAGTCC ATGTGCCCCC CTCAGCAGTA CGGTTTCCCT
    ACCCCCCCGT TTGGCACGCA GTGGAGCGGG ACGGGTGGCC CGGCACCCCA GCCGCTTAGC
    CAGTTCCAGC CTGTGGGAAC AGCCTCCTTA CAGAATTTCA ACATCAGCAA CTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848497.1
    CDS129..6650
    Translation

    Target ORF information:

    RefSeq Version XM_024992729.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GAGCACTCAG
    GTCGTCTCTC AGGTGGCTCC TCCAGAGCCA GCTCCCGGGG CTCAGCCACA GCCTGCCCAG
    CCAGCCGCGG TGGTCTCTTC TATAGACAGC GCACATTCAG ATGTGGCTTC AGGCCTGAGT
    GATGGCAACG AGAATGTCCC CTCATCCAGC GGGAGGCACG AGGGCAGGAC CACGAAGCGG
    CTGCATCGGA AGTCTATAAG GAGCCGCTCT CGTCACGAGA AGCCTTCCCG CCCCAAACTG
    AGAATTCTCA ATGTTTCAAA TAAGGGAGAT CGGGTGGTAG AATGTCAGTT AGAGACTCAC
    AATCGGAAGA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAACCCTGA GGAGATAGCC
    ACAATTATGG TGAACAATGA CTTTATTCTG GCACTGGAGA GAGATGCGTT TGTGGAGCAA
    GTGCGGGAGA TCATTGAGAA AGCTGACGAG ATGCTCAGTG AGGATGTCAG CGTGGAGCCA
    GAGGGTGACC AGAGCTTGGA GAGTCTCCAT GGCAAGGATG ACTATGGCTT CTCAGGCACC
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCAT CTTCCTTGCC ACAACAGATA
    GCAGGCATTC CTGCCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTAAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAC TTTTCACTGA AATTTCAGAT
    ACGGTTGCTG CTTCCACATC TGCTGGCCCT GGAATGAACT TGTCTCACTC TGCCTCATCC
    CTTAGTCTGC AGCAGGCCTT TTCTGAACTT AGACATGCTC GGATGGCAGA AGGGCCGAGT
    ACGGCCCCTC CCCACTTTGG TCAGACAGGC CCCGTGTTTC CAGCAGTGCC TCCCACCCTG
    AGCAGTGCCC CTGGAGCCCC AGCCGCAGCA GCCGCAGCCT CGGTGTCAGC CCCTGCCCCA
    AGCCACCCTC TTAGTGACGT GACCACATCG GTCATTCAGA CTGAGGTGAC AGTGGCCCCT
    GGAACAGGTG TCATGCCTCC CAGTGGTCTC CCTGTACCCC CTGTGTCTGA GTCCCTGGCA
    CCTTCCAGCG TGGCCCCCAG CGTCACCATC CCTGCAGTTC TCTCGGCACT ACCAGCGCCC
    CAGGCGGCAC AGGCTCCCAC CCCTGGCAGC GGGGCCTCCA GTGCAGGCAC TTTCCCCTCT
    GCACCCGCAT CCCTGACACC AGCCTCTGCT GTGGGCAGCG CTGTTGCCTC TGCTGCTCAG
    CTTCCGCCTG TGCCGTCTCA GCCCGCGGTA GGCAGCTCGA CCGGGGTGGC CACGCTGACA
    GCCGTGCCCG CTGCCGCTCT GGGCCCTAGC CTCACGGCAC TGCCGTCCCT TGCTCTCAGC
    AGCAGTACCT CTGCCCCAGC TCTGGCAGAG ACAGTGCTGG TCAGTGCCCA CGCACTGGAT
    AAGACGGCCC ATAGTAGCAC CACTGGCCTG GCTCTGTCCC TTCCTGCCAC CTCGTCCTCT
    CCCGCCCCTG GAGCAGGAGT GTCCAGTGCT GTTTCTCAGC CTACTGCTGC GGCTCACCCT
    TTGGTCCTCC CATCAGTCGT AACCTCTACT CCCGTACTCT CCCAGGCAGG ACCCATGTCA
    GCACCTCTAC TGCCCCAGGT ACCCGGTATT CCACCCCTGG TACAACCTGT TGTCAGCGTG
    CCTGCTGTGC AACAGACCCT GGTTCATAGT CAGCCTCAGC CCGCTCCACT CCCCAACCAG
    CCCCACACTC ACTGTCCCGA GATAGACGCC GACACTCAAC CCAAAGCTCC CGGGATCGAC
    GACATCAAGA CTTTGGAGGA AAAGCTGCGA TCTCTCTTTA GTGAGCACAG TTCTTCTGGA
    GCCCAACACG CCCCCGTCTC TCTGGAGACC TCGCTCACTG TAGAGACCGC TGTCACAGCA
    GGCCTCCCAA CCACTGCTGT TGCACCAAGC AAGCCTCTGA CTTCCACTGC CAGTACTTGC
    TTGCCACCAA CCAGTCTGCC CTTAGGAGCA GCTGGCTTGT CAGTTATACC AATGGTCACT
    CCTGGTCAAG TTTCCACCCC AGTCAGCTCT GCGCCAGGAG TGAAACTTGA AACTGCTCCA
    GCAAAGCCAC CTTTAACTAA GCCTCCAGTG CTGCCCATGG GCACTGAACT TCCAGCTGGT
    ACTCCACCCA GCGAGCAGCT GCCACCTTTT CCAGGACCTT CACTAACTCA GCCCCCGCAG
    CCCCTGGGAG ACCTGGACGC TCAGCTGAGA AGAACCCTCA GTCCCGAGAG TGGCATGCTG
    ACGTCTGCCG TTGGACCCGT GTCTGTGGTG GCTCCAACAG CAGTTGTGGA GACCGAAGCC
    CAACTTCCAA AGGATGTTCT TTCAGTCTCT CAAGTCACAG AAGCTCCTGT CCTTGCGCCC
    ACTTCCACAG CTGGTGTTCT GAAGATGGGG CGATTTCAGG TCTCAGTTGC AATGGATGAC
    CCCCAGAAGG AGGGTAAAAG TAAGTCGGAG GATGCCAAGT CTGTCCACTT CGCATCCAGC
    ACGTCAGAGT CCTCGGTGCT GTCAAGCAGC AGCCCCGAGA GCACCGTGGT GAAAGCAGAG
    CCCAACGGGC TGGCCGTCCG GGGCGCCTCT GCAGACATGC CTGACAGCGC CCACCAGCCA
    CCTGCCTCAG AAGCTGGGCA GCCTAGCAAG GTGGGCCGTT TTCAGGTGAC CACCACAGCG
    AGCAGAGTGG GTCGCTTCTC TGTGTCCAGA ACTGAAGACA AGATGGCTGA GGTGAAGGGA
    GAGGAGCCAG TGGTCTCTCC TTTCATGGAC TCAGAACACG ACATTCCTCC CACCGTGACG
    CCAAAGAAAG AGAAACCCGA ACTGTCTGAG CCTTCGCATC TGAACGGGCC GTCTTCCGAC
    CTGGAGGCTG CCTTCCTCAG CCGGGACGTG GATGCTGGCT CAGGGAGCCC TCACTCCCCT
    CCGCAGCTGA GCTCCAAGAG CCTTCCTGCC CAGAGCCTCA GTCAGAGCCT CAGCAATTCC
    TTCAACTCTT CCTACATGAG CAGCGACAAT GAGTCAGACA TTGAAGACGA GGACTTAAAG
    TTAGAGCTTC GCCGACTACG GGAAAAACAT CTTAAAGAGA TTCAGGACCT GCAGAGTCGC
    CAGAAGCACG AAATTGAGTC TTTATACACC AAACTGGGCA AGGTTCCTCC TGCTGTCATC
    ATTCCCCCAG CAGCCCCCCT TGCAGGGAGA AGGAGGCGAC CCACCAAAAG CAAAGGGAGC
    AAGTCCAGTC GCAGCAGCTC TTTGGGGAAT AAAAGCCCTG GACCAGGTAC CCTGTCTGGC
    CAGAGTGCAG CTTCAGTCTT GCACCCTCAG CAGACCCTCC CCGCTCCTGG CAGCGTCCCG
    GAGACAGGGC CCAACCAGCT GTTACAGCCT GTGAAGCCAT CTCCTTCCAG TGACAACCTG
    TACTCGGCCT TTACCAGCGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC GCCAGGTCAA
    GGCTGTGCCA AGTTTAACTG TGCGTCCGAG CAGGTGACGT TCAAAGCTGG CGGCAGGAGG
    ACGCGATTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGCCCT
    TGCAACCAGC TCTGTAGGAC CAGCAGCACC AACGCCGTTG GGGGAGCCGT GAGCAGCCAG
    GCCGCCCCGG CTCCACCTCC TGCCGTGACG TCCAGCAGGA AGGGCATGTT CACGGACGAC
    CTGCACAAGC TGGTGGACAA CTGGGCCCGG GACGCCATGA GTCTCTCAGG CAGGCGGGGA
    AGCAAAGGAC ACGTGAGCTA TGAGGGCCCT GGCATGGCAA GGAAGTTCTC TGCGCCCGGT
    CAGCTGTGCG TCTCCATGAC CTCAAACCTG GGCGGCTCTG CCCCCGGCTC TGCAGCGTCA
    GCTACCTCTC TAGGGCACTT CCCCAAGTCC ATGTGCCCCC CTCAGCAGTA CGGTTTCCCT
    ACCCCCCCGT TTGGCACGCA GTGGAGCGGG ACGGGTGGCC CGGCACCCCA GCCGCTTAGC
    CAGTTCCAGC CTGTGGGAAC AGCCTCCTTA CAGAATTTCA ACATCAGCAA CTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234928
    Clone ID Related Accession (Same CDS sequence) XM_024992730.1
    Accession Version XM_024992730.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GAGCACTCAG
    GTCGTCTCTC AGGTGGCTCC TCCAGAGCCA GCTCCCGGGG CTCAGCCACA GCCTGCCCAG
    CCAGCCGCGG TGGTCTCTTC TATAGACAGC GCACATTCAG ATGTGGCTTC AGGCCTGAGT
    GATGGCAACG AGAATGTCCC CTCATCCAGC GGGAGGCACG AGGGCAGGAC CACGAAGCGG
    CTGCATCGGA AGTCTATAAG GAGCCGCTCT CGTCACGAGA AGCCTTCCCG CCCCAAACTG
    AGAATTCTCA ATGTTTCAAA TAAGGGAGAT CGGGTGGTAG AATGTCAGTT AGAGACTCAC
    AATCGGAAGA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAACCCTGA GGAGATAGCC
    ACAATTATGG TGAACAATGA CTTTATTCTG GCACTGGAGA GAGATGCGTT TGTGGAGCAA
    GTGCGGGAGA TCATTGAGAA AGCTGACGAG ATGCTCAGTG AGGATGTCAG CGTGGAGCCA
    GAGGGTGACC AGAGCTTGGA GAGTCTCCAT GGCAAGGATG ACTATGGCTT CTCAGGCACC
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCAT CTTCCTTGCC ACAACAGATA
    GGCATTCCTG CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTA
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAACTTT TCACTGAAAT TTCAGATACG
    GTTGCTGCTT CCACATCTGC TGGCCCTGGA ATGAACTTGT CTCACTCTGC CTCATCCCTT
    AGTCTGCAGC AGGCCTTTTC TGAACTTAGA CATGCTCGGA TGGCAGAAGG GCCGAGTACG
    GCCCCTCCCC ACTTTGGTCA GACAGGCCCC GTGTTTCCAG CAGTGCCTCC CACCCTGAGC
    AGTGCCCCTG GAGCCCCAGC CGCAGCAGCC GCAGCCTCGG TGTCAGCCCC TGCCCCAAGC
    CACCCTCTTA GTGACGTGAC CACATCGGTC ATTCAGACTG AGGTGACAGT GGCCCCTGGA
    ACAGGTGTCA TGCCTCCCAG TGGTCTCCCT GTACCCCCTG TGTCTGAGTC CCTGGCACCT
    TCCAGCGTGG CCCCCAGCGT CACCATCCCT GCAGTTCTCT CGGCACTACC AGCGCCCCAG
    GCGGCACAGG CTCCCACCCC TGGCAGCGGG GCCTCCAGTG CAGGCACTTT CCCCTCTGCA
    CCCGCATCCC TGACACCAGC CTCTGCTGTG GGCAGCGCTG TTGCCTCTGC TGCTCAGCTT
    CCGCCTGTGC CGTCTCAGCC CGCGGTAGGC AGCTCGACCG GGGTGGCCAC GCTGACAGCC
    GTGCCCGCTG CCGCTCTGGG CCCTAGCCTC ACGGCACTGC CGTCCCTTGC TCTCAGCAGC
    AGTACCTCTG CCCCAGCTCT GGCAGAGACA GTGCTGGTCA GTGCCCACGC ACTGGATAAG
    ACGGCCCATA GTAGCACCAC TGGCCTGGCT CTGTCCCTTC CTGCCACCTC GTCCTCTCCC
    GCCCCTGGAG CAGGAGTGTC CAGTGCTGTT TCTCAGCCTA CTGCTGCGGC TCACCCTTTG
    GTCCTCCCAT CAGTCGTAAC CTCTACTCCC GTACTCTCCC AGGCAGGACC CATGTCAGCA
    CCTCTACTGC CCCAGGTACC CGGTATTCCA CCCCTGGTAC AACCTGTTGT CAGCGTGCCT
    GCTGTGCAAC AGACCCTGGT TCATAGTCAG CCTCAGCCCG CTCCACTCCC CAACCAGCCC
    CACACTCACT GTCCCGAGAT AGACGCCGAC ACTCAACCCA AAGCTCCCGG GATCGACGAC
    ATCAAGACTT TGGAGGAAAA GCTGCGATCT CTCTTTAGTG AGCACAGTTC TTCTGGAGCC
    CAACACGCCC CCGTCTCTCT GGAGACCTCG CTCACTGTAG AGACCGCTGT CACAGCAGGC
    CTCCCAACCA CTGCTGTTGC ACCAAGCAAG CCTCTGACTT CCACTGCCAG TACTTGCTTG
    CCACCAACCA GTCTGCCCTT AGGAGCAGCT GGCTTGTCAG TTATACCAAT GGTCACTCCT
    GGTCAAGTTT CCACCCCAGT CAGCTCTGCG CCAGGAGTGA AACTTGAAAC TGCTCCAGCA
    AAGCCACCTT TAACTAAGCC TCCAGTGCTG CCCATGGGCA CTGAACTTCC AGCTGGTACT
    CCACCCAGCG AGCAGCTGCC ACCTTTTCCA GGACCTTCAC TAACTCAGCC CCCGCAGCCC
    CTGGGAGACC TGGACGCTCA GCTGAGAAGA ACCCTCAGTC CCGAGAGTGG CATGCTGACG
    TCTGCCGTTG GACCCGTGTC TGTGGTGGCT CCAACAGCAG TTGTGGAGAC CGAAGCCCAA
    CTTCCAAAGG ATGTTCTTTC AGTCTCTCAA GTCACAGAAG CTCCTGTCCT TGCGCCCACT
    TCCACAGCTG GTGTTCTGAA GATGGGGCGA TTTCAGGTCT CAGTTGCAAT GGATGACCCC
    CAGAAGGAGG GTAAAAGTAA GTCGGAGGAT GCCAAGTCTG TCCACTTCGC ATCCAGCACG
    TCAGAGTCCT CGGTGCTGTC AAGCAGCAGC CCCGAGAGCA CCGTGGTGAA AGCAGAGCCC
    AACGGGCTGG CCGTCCGGGG CGCCTCTGCA GACATGCCTG ACAGCGCCCA CCAGCCACCT
    GCCTCAGAAG CTGGGCAGCC TAGCAAGGTG GGCCGTTTTC AGGTGACCAC CACAGCGAGC
    AGAGTGGGTC GCTTCTCTGT GTCCAGAACT GAAGACAAGA TGGCTGAGGT GAAGGGAGAG
    GAGCCAGTGG TCTCTCCTTT CATGGACTCA GAACACGACA TTCCTCCCAC CGTGACGCCA
    AAGAAAGAGA AACCCGAACT GTCTGAGCCT TCGCATCTGA ACGGGCCGTC TTCCGACCTG
    GAGGCTGCCT TCCTCAGCCG GGACGTGGAT GCTGGCTCAG GGAGCCCTCA CTCCCCTCCG
    CAGCTGAGCT CCAAGAGCCT TCCTGCCCAG AGCCTCAGTC AGAGCCTCAG CAATTCCTTC
    AACTCTTCCT ACATGAGCAG CGACAATGAG TCAGACATTG AAGACGAGGA CTTAAAGTTA
    GAGCTTCGCC GACTACGGGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCACGAAA TTGAGTCTTT ATACACCAAA CTGGGCAAGG TTCCTCCTGC TGTCATCATT
    CCCCCAGCAG CCCCCCTTGC AGGGAGAAGG AGGCGACCCA CCAAAAGCAA AGGGAGCAAG
    TCCAGTCGCA GCAGCTCTTT GGGGAATAAA AGCCCTGGAC CAGGTACCCT GTCTGGCCAG
    AGTGCAGCTT CAGTCTTGCA CCCTCAGCAG ACCCTCCCCG CTCCTGGCAG CGTCCCGGAG
    ACAGGGCCCA ACCAGCTGTT ACAGCCTGTG AAGCCATCTC CTTCCAGTGA CAACCTGTAC
    TCGGCCTTTA CCAGCGATGG TGCCATTTCA GTACCAAGCC TTTCTGCGCC AGGTCAAGGC
    TGTGCCAAGT TTAACTGTGC GTCCGAGCAG GTGACGTTCA AAGCTGGCGG CAGGAGGACG
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCTTGC
    AACCAGCTCT GTAGGACCAG CAGCACCAAC GCCGTTGGGG GAGCCGTGAG CAGCCAGGCC
    GCCCCGGCTC CACCTCCTGC CGTGACGTCC AGCAGGAAGG GCATGTTCAC GGACGACCTG
    CACAAGCTGG TGGACAACTG GGCCCGGGAC GCCATGAGTC TCTCAGGCAG GCGGGGAAGC
    AAAGGACACG TGAGCTATGA GGGCCCTGGC ATGGCAAGGA AGTTCTCTGC GCCCGGTCAG
    CTGTGCGTCT CCATGACCTC AAACCTGGGC GGCTCTGCCC CCGGCTCTGC AGCGTCAGCT
    ACCTCTCTAG GGCACTTCCC CAAGTCCATG TGCCCCCCTC AGCAGTACGG TTTCCCTACC
    CCCCCGTTTG GCACGCAGTG GAGCGGGACG GGTGGCCCGG CACCCCAGCC GCTTAGCCAG
    TTCCAGCCTG TGGGAACAGC CTCCTTACAG AATTTCAACA TCAGCAACTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848498.1
    CDS129..6647
    Translation

    Target ORF information:

    RefSeq Version XM_024992730.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAGCAAGGA GTGCAGCCAA CAGCCCCCTC TCAGCAGGCG
    GTGCAGTATC CGCTCCCTCA GACATCGGCC CCCAGCGAGG CCACGATTGC ACAGCCTGTG
    AGTCAGCCTC CACCTCCACA GGCCTTGCCA CACGTGTCCG CGGGAAAACA GAGCACTCAG
    GTCGTCTCTC AGGTGGCTCC TCCAGAGCCA GCTCCCGGGG CTCAGCCACA GCCTGCCCAG
    CCAGCCGCGG TGGTCTCTTC TATAGACAGC GCACATTCAG ATGTGGCTTC AGGCCTGAGT
    GATGGCAACG AGAATGTCCC CTCATCCAGC GGGAGGCACG AGGGCAGGAC CACGAAGCGG
    CTGCATCGGA AGTCTATAAG GAGCCGCTCT CGTCACGAGA AGCCTTCCCG CCCCAAACTG
    AGAATTCTCA ATGTTTCAAA TAAGGGAGAT CGGGTGGTAG AATGTCAGTT AGAGACTCAC
    AATCGGAAGA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAACCCTGA GGAGATAGCC
    ACAATTATGG TGAACAATGA CTTTATTCTG GCACTGGAGA GAGATGCGTT TGTGGAGCAA
    GTGCGGGAGA TCATTGAGAA AGCTGACGAG ATGCTCAGTG AGGATGTCAG CGTGGAGCCA
    GAGGGTGACC AGAGCTTGGA GAGTCTCCAT GGCAAGGATG ACTATGGCTT CTCAGGCACC
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCAT CTTCCTTGCC ACAACAGATA
    GGCATTCCTG CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTA
    AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAACTTT TCACTGAAAT TTCAGATACG
    GTTGCTGCTT CCACATCTGC TGGCCCTGGA ATGAACTTGT CTCACTCTGC CTCATCCCTT
    AGTCTGCAGC AGGCCTTTTC TGAACTTAGA CATGCTCGGA TGGCAGAAGG GCCGAGTACG
    GCCCCTCCCC ACTTTGGTCA GACAGGCCCC GTGTTTCCAG CAGTGCCTCC CACCCTGAGC
    AGTGCCCCTG GAGCCCCAGC CGCAGCAGCC GCAGCCTCGG TGTCAGCCCC TGCCCCAAGC
    CACCCTCTTA GTGACGTGAC CACATCGGTC ATTCAGACTG AGGTGACAGT GGCCCCTGGA
    ACAGGTGTCA TGCCTCCCAG TGGTCTCCCT GTACCCCCTG TGTCTGAGTC CCTGGCACCT
    TCCAGCGTGG CCCCCAGCGT CACCATCCCT GCAGTTCTCT CGGCACTACC AGCGCCCCAG
    GCGGCACAGG CTCCCACCCC TGGCAGCGGG GCCTCCAGTG CAGGCACTTT CCCCTCTGCA
    CCCGCATCCC TGACACCAGC CTCTGCTGTG GGCAGCGCTG TTGCCTCTGC TGCTCAGCTT
    CCGCCTGTGC CGTCTCAGCC CGCGGTAGGC AGCTCGACCG GGGTGGCCAC GCTGACAGCC
    GTGCCCGCTG CCGCTCTGGG CCCTAGCCTC ACGGCACTGC CGTCCCTTGC TCTCAGCAGC
    AGTACCTCTG CCCCAGCTCT GGCAGAGACA GTGCTGGTCA GTGCCCACGC ACTGGATAAG
    ACGGCCCATA GTAGCACCAC TGGCCTGGCT CTGTCCCTTC CTGCCACCTC GTCCTCTCCC
    GCCCCTGGAG CAGGAGTGTC CAGTGCTGTT TCTCAGCCTA CTGCTGCGGC TCACCCTTTG
    GTCCTCCCAT CAGTCGTAAC CTCTACTCCC GTACTCTCCC AGGCAGGACC CATGTCAGCA
    CCTCTACTGC CCCAGGTACC CGGTATTCCA CCCCTGGTAC AACCTGTTGT CAGCGTGCCT
    GCTGTGCAAC AGACCCTGGT TCATAGTCAG CCTCAGCCCG CTCCACTCCC CAACCAGCCC
    CACACTCACT GTCCCGAGAT AGACGCCGAC ACTCAACCCA AAGCTCCCGG GATCGACGAC
    ATCAAGACTT TGGAGGAAAA GCTGCGATCT CTCTTTAGTG AGCACAGTTC TTCTGGAGCC
    CAACACGCCC CCGTCTCTCT GGAGACCTCG CTCACTGTAG AGACCGCTGT CACAGCAGGC
    CTCCCAACCA CTGCTGTTGC ACCAAGCAAG CCTCTGACTT CCACTGCCAG TACTTGCTTG
    CCACCAACCA GTCTGCCCTT AGGAGCAGCT GGCTTGTCAG TTATACCAAT GGTCACTCCT
    GGTCAAGTTT CCACCCCAGT CAGCTCTGCG CCAGGAGTGA AACTTGAAAC TGCTCCAGCA
    AAGCCACCTT TAACTAAGCC TCCAGTGCTG CCCATGGGCA CTGAACTTCC AGCTGGTACT
    CCACCCAGCG AGCAGCTGCC ACCTTTTCCA GGACCTTCAC TAACTCAGCC CCCGCAGCCC
    CTGGGAGACC TGGACGCTCA GCTGAGAAGA ACCCTCAGTC CCGAGAGTGG CATGCTGACG
    TCTGCCGTTG GACCCGTGTC TGTGGTGGCT CCAACAGCAG TTGTGGAGAC CGAAGCCCAA
    CTTCCAAAGG ATGTTCTTTC AGTCTCTCAA GTCACAGAAG CTCCTGTCCT TGCGCCCACT
    TCCACAGCTG GTGTTCTGAA GATGGGGCGA TTTCAGGTCT CAGTTGCAAT GGATGACCCC
    CAGAAGGAGG GTAAAAGTAA GTCGGAGGAT GCCAAGTCTG TCCACTTCGC ATCCAGCACG
    TCAGAGTCCT CGGTGCTGTC AAGCAGCAGC CCCGAGAGCA CCGTGGTGAA AGCAGAGCCC
    AACGGGCTGG CCGTCCGGGG CGCCTCTGCA GACATGCCTG ACAGCGCCCA CCAGCCACCT
    GCCTCAGAAG CTGGGCAGCC TAGCAAGGTG GGCCGTTTTC AGGTGACCAC CACAGCGAGC
    AGAGTGGGTC GCTTCTCTGT GTCCAGAACT GAAGACAAGA TGGCTGAGGT GAAGGGAGAG
    GAGCCAGTGG TCTCTCCTTT CATGGACTCA GAACACGACA TTCCTCCCAC CGTGACGCCA
    AAGAAAGAGA AACCCGAACT GTCTGAGCCT TCGCATCTGA ACGGGCCGTC TTCCGACCTG
    GAGGCTGCCT TCCTCAGCCG GGACGTGGAT GCTGGCTCAG GGAGCCCTCA CTCCCCTCCG
    CAGCTGAGCT CCAAGAGCCT TCCTGCCCAG AGCCTCAGTC AGAGCCTCAG CAATTCCTTC
    AACTCTTCCT ACATGAGCAG CGACAATGAG TCAGACATTG AAGACGAGGA CTTAAAGTTA
    GAGCTTCGCC GACTACGGGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCACGAAA TTGAGTCTTT ATACACCAAA CTGGGCAAGG TTCCTCCTGC TGTCATCATT
    CCCCCAGCAG CCCCCCTTGC AGGGAGAAGG AGGCGACCCA CCAAAAGCAA AGGGAGCAAG
    TCCAGTCGCA GCAGCTCTTT GGGGAATAAA AGCCCTGGAC CAGGTACCCT GTCTGGCCAG
    AGTGCAGCTT CAGTCTTGCA CCCTCAGCAG ACCCTCCCCG CTCCTGGCAG CGTCCCGGAG
    ACAGGGCCCA ACCAGCTGTT ACAGCCTGTG AAGCCATCTC CTTCCAGTGA CAACCTGTAC
    TCGGCCTTTA CCAGCGATGG TGCCATTTCA GTACCAAGCC TTTCTGCGCC AGGTCAAGGC
    TGTGCCAAGT TTAACTGTGC GTCCGAGCAG GTGACGTTCA AAGCTGGCGG CAGGAGGACG
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCTTGC
    AACCAGCTCT GTAGGACCAG CAGCACCAAC GCCGTTGGGG GAGCCGTGAG CAGCCAGGCC
    GCCCCGGCTC CACCTCCTGC CGTGACGTCC AGCAGGAAGG GCATGTTCAC GGACGACCTG
    CACAAGCTGG TGGACAACTG GGCCCGGGAC GCCATGAGTC TCTCAGGCAG GCGGGGAAGC
    AAAGGACACG TGAGCTATGA GGGCCCTGGC ATGGCAAGGA AGTTCTCTGC GCCCGGTCAG
    CTGTGCGTCT CCATGACCTC AAACCTGGGC GGCTCTGCCC CCGGCTCTGC AGCGTCAGCT
    ACCTCTCTAG GGCACTTCCC CAAGTCCATG TGCCCCCCTC AGCAGTACGG TTTCCCTACC
    CCCCCGTTTG GCACGCAGTG GAGCGGGACG GGTGGCCCGG CACCCCAGCC GCTTAGCCAG
    TTCCAGCCTG TGGGAACAGC CTCCTTACAG AATTTCAACA TCAGCAACTT GCAGAAATCC
    ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234929
    Clone ID Related Accession (Same CDS sequence) XM_024992731.1
    Accession Version XM_024992731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAAGGAGTG CAGCCAACAG CCCCCTCTCA GCAGGCGGTG
    CAGTATCCGC TCCCTCAGAC ATCGGCCCCC AGCGAGGCCA CGATTGCACA GCCTGTGAGT
    CAGCCTCCAC CTCCACAGGC CTTGCCACAC GTGTCCGCGG GAAAACAGAG CACTCAGGTC
    GTCTCTCAGG TGGCTCCTCC AGAGCCAGCT CCCGGGGCTC AGCCACAGCC TGCCCAGCCA
    GCCGCGGTGG TCTCTTCTAT AGACAGCGCA CATTCAGATG TGGCTTCAGG CCTGAGTGAT
    GGCAACGAGA ATGTCCCCTC ATCCAGCGGG AGGCACGAGG GCAGGACCAC GAAGCGGCTG
    CATCGGAAGT CTATAAGGAG CCGCTCTCGT CACGAGAAGC CTTCCCGCCC CAAACTGAGA
    ATTCTCAATG TTTCAAATAA GGGAGATCGG GTGGTAGAAT GTCAGTTAGA GACTCACAAT
    CGGAAGATGG TTACATTCAA ATTTGATTTA GATGGTGACA ACCCTGAGGA GATAGCCACA
    ATTATGGTGA ACAATGACTT TATTCTGGCA CTGGAGAGAG ATGCGTTTGT GGAGCAAGTG
    CGGGAGATCA TTGAGAAAGC TGACGAGATG CTCAGTGAGG ATGTCAGCGT GGAGCCAGAG
    GGTGACCAGA GCTTGGAGAG TCTCCATGGC AAGGATGACT ATGGCTTCTC AGGCACCCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCATCTT CCTTGCCACA ACAGATAGGC
    ATTCCTGCCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTAAGT
    CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAACTTTTCA CTGAAATTTC AGATACGGTT
    GCTGCTTCCA CATCTGCTGG CCCTGGAATG AACTTGTCTC ACTCTGCCTC ATCCCTTAGT
    CTGCAGCAGG CCTTTTCTGA ACTTAGACAT GCTCGGATGG CAGAAGGGCC GAGTACGGCC
    CCTCCCCACT TTGGTCAGAC AGGCCCCGTG TTTCCAGCAG TGCCTCCCAC CCTGAGCAGT
    GCCCCTGGAG CCCCAGCCGC AGCAGCCGCA GCCTCGGTGT CAGCCCCTGC CCCAAGCCAC
    CCTCTTAGTG ACGTGACCAC ATCGGTCATT CAGACTGAGG TGACAGTGGC CCCTGGAACA
    GGTGTCATGC CTCCCAGTGG TCTCCCTGTA CCCCCTGTGT CTGAGTCCCT GGCACCTTCC
    AGCGTGGCCC CCAGCGTCAC CATCCCTGCA GTTCTCTCGG CACTACCAGC GCCCCAGGCG
    GCACAGGCTC CCACCCCTGG CAGCGGGGCC TCCAGTGCAG GCACTTTCCC CTCTGCACCC
    GCATCCCTGA CACCAGCCTC TGCTGTGGGC AGCGCTGTTG CCTCTGCTGC TCAGCTTCCG
    CCTGTGCCGT CTCAGCCCGC GGTAGGCAGC TCGACCGGGG TGGCCACGCT GACAGCCGTG
    CCCGCTGCCG CTCTGGGCCC TAGCCTCACG GCACTGCCGT CCCTTGCTCT CAGCAGCAGT
    ACCTCTGCCC CAGCTCTGGC AGAGACAGTG CTGGTCAGTG CCCACGCACT GGATAAGACG
    GCCCATAGTA GCACCACTGG CCTGGCTCTG TCCCTTCCTG CCACCTCGTC CTCTCCCGCC
    CCTGGAGCAG GAGTGTCCAG TGCTGTTTCT CAGCCTACTG CTGCGGCTCA CCCTTTGGTC
    CTCCCATCAG TCGTAACCTC TACTCCCGTA CTCTCCCAGG CAGGACCCAT GTCAGCACCT
    CTACTGCCCC AGGTACCCGG TATTCCACCC CTGGTACAAC CTGTTGTCAG CGTGCCTGCT
    GTGCAACAGA CCCTGGTTCA TAGTCAGCCT CAGCCCGCTC CACTCCCCAA CCAGCCCCAC
    ACTCACTGTC CCGAGATAGA CGCCGACACT CAACCCAAAG CTCCCGGGAT CGACGACATC
    AAGACTTTGG AGGAAAAGCT GCGATCTCTC TTTAGTGAGC ACAGTTCTTC TGGAGCCCAA
    CACGCCCCCG TCTCTCTGGA GACCTCGCTC ACTGTAGAGA CCGCTGTCAC AGCAGGCCTC
    CCAACCACTG CTGTTGCACC AAGCAAGCCT CTGACTTCCA CTGCCAGTAC TTGCTTGCCA
    CCAACCAGTC TGCCCTTAGG AGCAGCTGGC TTGTCAGTTA TACCAATGGT CACTCCTGGT
    CAAGTTTCCA CCCCAGTCAG CTCTGCGCCA GGAGTGAAAC TTGAAACTGC TCCAGCAAAG
    CCACCTTTAA CTAAGCCTCC AGTGCTGCCC ATGGGCACTG AACTTCCAGC TGGTACTCCA
    CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCACTAA CTCAGCCCCC GCAGCCCCTG
    GGAGACCTGG ACGCTCAGCT GAGAAGAACC CTCAGTCCCG AGAGTGGCAT GCTGACGTCT
    GCCGTTGGAC CCGTGTCTGT GGTGGCTCCA ACAGCAGTTG TGGAGACCGA AGCCCAACTT
    CCAAAGGATG TTCTTTCAGT CTCTCAAGTC ACAGAAGCTC CTGTCCTTGC GCCCACTTCC
    ACAGCTGGTG TTCTGAAGAT GGGGCGATTT CAGGTCTCAG TTGCAATGGA TGACCCCCAG
    AAGGAGGGTA AAAGTAAGTC GGAGGATGCC AAGTCTGTCC ACTTCGCATC CAGCACGTCA
    GAGTCCTCGG TGCTGTCAAG CAGCAGCCCC GAGAGCACCG TGGTGAAAGC AGAGCCCAAC
    GGGCTGGCCG TCCGGGGCGC CTCTGCAGAC ATGCCTGACA GCGCCCACCA GCCACCTGCC
    TCAGAAGCTG GGCAGCCTAG CAAGGTGGGC CGTTTTCAGG TGACCACCAC AGCGAGCAGA
    GTGGGTCGCT TCTCTGTGTC CAGAACTGAA GACAAGATGG CTGAGGTGAA GGGAGAGGAG
    CCAGTGGTCT CTCCTTTCAT GGACTCAGAA CACGACATTC CTCCCACCGT GACGCCAAAG
    AAAGAGAAAC CCGAACTGTC TGAGCCTTCG CATCTGAACG GGCCGTCTTC CGACCTGGAG
    GCTGCCTTCC TCAGCCGGGA CGTGGATGCT GGCTCAGGGA GCCCTCACTC CCCTCCGCAG
    CTGAGCTCCA AGAGCCTTCC TGCCCAGAGC CTCAGTCAGA GCCTCAGCAA TTCCTTCAAC
    TCTTCCTACA TGAGCAGCGA CAATGAGTCA GACATTGAAG ACGAGGACTT AAAGTTAGAG
    CTTCGCCGAC TACGGGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CACGAAATTG AGTCTTTATA CACCAAACTG GGCAAGGTTC CTCCTGCTGT CATCATTCCC
    CCAGCAGCCC CCCTTGCAGG GAGAAGGAGG CGACCCACCA AAAGCAAAGG GAGCAAGTCC
    AGTCGCAGCA GCTCTTTGGG GAATAAAAGC CCTGGACCAG GTACCCTGTC TGGCCAGAGT
    GCAGCTTCAG TCTTGCACCC TCAGCAGACC CTCCCCGCTC CTGGCAGCGT CCCGGAGACA
    GGGCCCAACC AGCTGTTACA GCCTGTGAAG CCATCTCCTT CCAGTGACAA CCTGTACTCG
    GCCTTTACCA GCGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCGCCAGG TCAAGGCTGT
    GCCAAGTTTA ACTGTGCGTC CGAGCAGGTG ACGTTCAAAG CTGGCGGCAG GAGGACGCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG CCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACCAACGCC GTTGGGGGAG CCGTGAGCAG CCAGGCCGCC
    CCGGCTCCAC CTCCTGCCGT GACGTCCAGC AGGAAGGGCA TGTTCACGGA CGACCTGCAC
    AAGCTGGTGG ACAACTGGGC CCGGGACGCC ATGAGTCTCT CAGGCAGGCG GGGAAGCAAA
    GGACACGTGA GCTATGAGGG CCCTGGCATG GCAAGGAAGT TCTCTGCGCC CGGTCAGCTG
    TGCGTCTCCA TGACCTCAAA CCTGGGCGGC TCTGCCCCCG GCTCTGCAGC GTCAGCTACC
    TCTCTAGGGC ACTTCCCCAA GTCCATGTGC CCCCCTCAGC AGTACGGTTT CCCTACCCCC
    CCGTTTGGCA CGCAGTGGAG CGGGACGGGT GGCCCGGCAC CCCAGCCGCT TAGCCAGTTC
    CAGCCTGTGG GAACAGCCTC CTTACAGAAT TTCAACATCA GCAACTTGCA GAAATCCATC
    AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848499.1
    CDS129..6644
    Translation

    Target ORF information:

    RefSeq Version XM_024992731.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCTTCA AGTAGTTTAA CAGGGGTTCC ATCCTCCCAA
    CCCATGCAGC ATTCTCAGCA GCAAGGAGTG CAGCCAACAG CCCCCTCTCA GCAGGCGGTG
    CAGTATCCGC TCCCTCAGAC ATCGGCCCCC AGCGAGGCCA CGATTGCACA GCCTGTGAGT
    CAGCCTCCAC CTCCACAGGC CTTGCCACAC GTGTCCGCGG GAAAACAGAG CACTCAGGTC
    GTCTCTCAGG TGGCTCCTCC AGAGCCAGCT CCCGGGGCTC AGCCACAGCC TGCCCAGCCA
    GCCGCGGTGG TCTCTTCTAT AGACAGCGCA CATTCAGATG TGGCTTCAGG CCTGAGTGAT
    GGCAACGAGA ATGTCCCCTC ATCCAGCGGG AGGCACGAGG GCAGGACCAC GAAGCGGCTG
    CATCGGAAGT CTATAAGGAG CCGCTCTCGT CACGAGAAGC CTTCCCGCCC CAAACTGAGA
    ATTCTCAATG TTTCAAATAA GGGAGATCGG GTGGTAGAAT GTCAGTTAGA GACTCACAAT
    CGGAAGATGG TTACATTCAA ATTTGATTTA GATGGTGACA ACCCTGAGGA GATAGCCACA
    ATTATGGTGA ACAATGACTT TATTCTGGCA CTGGAGAGAG ATGCGTTTGT GGAGCAAGTG
    CGGGAGATCA TTGAGAAAGC TGACGAGATG CTCAGTGAGG ATGTCAGCGT GGAGCCAGAG
    GGTGACCAGA GCTTGGAGAG TCTCCATGGC AAGGATGACT ATGGCTTCTC AGGCACCCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCATCTT CCTTGCCACA ACAGATAGGC
    ATTCCTGCCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTAAGT
    CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAACTTTTCA CTGAAATTTC AGATACGGTT
    GCTGCTTCCA CATCTGCTGG CCCTGGAATG AACTTGTCTC ACTCTGCCTC ATCCCTTAGT
    CTGCAGCAGG CCTTTTCTGA ACTTAGACAT GCTCGGATGG CAGAAGGGCC GAGTACGGCC
    CCTCCCCACT TTGGTCAGAC AGGCCCCGTG TTTCCAGCAG TGCCTCCCAC CCTGAGCAGT
    GCCCCTGGAG CCCCAGCCGC AGCAGCCGCA GCCTCGGTGT CAGCCCCTGC CCCAAGCCAC
    CCTCTTAGTG ACGTGACCAC ATCGGTCATT CAGACTGAGG TGACAGTGGC CCCTGGAACA
    GGTGTCATGC CTCCCAGTGG TCTCCCTGTA CCCCCTGTGT CTGAGTCCCT GGCACCTTCC
    AGCGTGGCCC CCAGCGTCAC CATCCCTGCA GTTCTCTCGG CACTACCAGC GCCCCAGGCG
    GCACAGGCTC CCACCCCTGG CAGCGGGGCC TCCAGTGCAG GCACTTTCCC CTCTGCACCC
    GCATCCCTGA CACCAGCCTC TGCTGTGGGC AGCGCTGTTG CCTCTGCTGC TCAGCTTCCG
    CCTGTGCCGT CTCAGCCCGC GGTAGGCAGC TCGACCGGGG TGGCCACGCT GACAGCCGTG
    CCCGCTGCCG CTCTGGGCCC TAGCCTCACG GCACTGCCGT CCCTTGCTCT CAGCAGCAGT
    ACCTCTGCCC CAGCTCTGGC AGAGACAGTG CTGGTCAGTG CCCACGCACT GGATAAGACG
    GCCCATAGTA GCACCACTGG CCTGGCTCTG TCCCTTCCTG CCACCTCGTC CTCTCCCGCC
    CCTGGAGCAG GAGTGTCCAG TGCTGTTTCT CAGCCTACTG CTGCGGCTCA CCCTTTGGTC
    CTCCCATCAG TCGTAACCTC TACTCCCGTA CTCTCCCAGG CAGGACCCAT GTCAGCACCT
    CTACTGCCCC AGGTACCCGG TATTCCACCC CTGGTACAAC CTGTTGTCAG CGTGCCTGCT
    GTGCAACAGA CCCTGGTTCA TAGTCAGCCT CAGCCCGCTC CACTCCCCAA CCAGCCCCAC
    ACTCACTGTC CCGAGATAGA CGCCGACACT CAACCCAAAG CTCCCGGGAT CGACGACATC
    AAGACTTTGG AGGAAAAGCT GCGATCTCTC TTTAGTGAGC ACAGTTCTTC TGGAGCCCAA
    CACGCCCCCG TCTCTCTGGA GACCTCGCTC ACTGTAGAGA CCGCTGTCAC AGCAGGCCTC
    CCAACCACTG CTGTTGCACC AAGCAAGCCT CTGACTTCCA CTGCCAGTAC TTGCTTGCCA
    CCAACCAGTC TGCCCTTAGG AGCAGCTGGC TTGTCAGTTA TACCAATGGT CACTCCTGGT
    CAAGTTTCCA CCCCAGTCAG CTCTGCGCCA GGAGTGAAAC TTGAAACTGC TCCAGCAAAG
    CCACCTTTAA CTAAGCCTCC AGTGCTGCCC ATGGGCACTG AACTTCCAGC TGGTACTCCA
    CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCACTAA CTCAGCCCCC GCAGCCCCTG
    GGAGACCTGG ACGCTCAGCT GAGAAGAACC CTCAGTCCCG AGAGTGGCAT GCTGACGTCT
    GCCGTTGGAC CCGTGTCTGT GGTGGCTCCA ACAGCAGTTG TGGAGACCGA AGCCCAACTT
    CCAAAGGATG TTCTTTCAGT CTCTCAAGTC ACAGAAGCTC CTGTCCTTGC GCCCACTTCC
    ACAGCTGGTG TTCTGAAGAT GGGGCGATTT CAGGTCTCAG TTGCAATGGA TGACCCCCAG
    AAGGAGGGTA AAAGTAAGTC GGAGGATGCC AAGTCTGTCC ACTTCGCATC CAGCACGTCA
    GAGTCCTCGG TGCTGTCAAG CAGCAGCCCC GAGAGCACCG TGGTGAAAGC AGAGCCCAAC
    GGGCTGGCCG TCCGGGGCGC CTCTGCAGAC ATGCCTGACA GCGCCCACCA GCCACCTGCC
    TCAGAAGCTG GGCAGCCTAG CAAGGTGGGC CGTTTTCAGG TGACCACCAC AGCGAGCAGA
    GTGGGTCGCT TCTCTGTGTC CAGAACTGAA GACAAGATGG CTGAGGTGAA GGGAGAGGAG
    CCAGTGGTCT CTCCTTTCAT GGACTCAGAA CACGACATTC CTCCCACCGT GACGCCAAAG
    AAAGAGAAAC CCGAACTGTC TGAGCCTTCG CATCTGAACG GGCCGTCTTC CGACCTGGAG
    GCTGCCTTCC TCAGCCGGGA CGTGGATGCT GGCTCAGGGA GCCCTCACTC CCCTCCGCAG
    CTGAGCTCCA AGAGCCTTCC TGCCCAGAGC CTCAGTCAGA GCCTCAGCAA TTCCTTCAAC
    TCTTCCTACA TGAGCAGCGA CAATGAGTCA GACATTGAAG ACGAGGACTT AAAGTTAGAG
    CTTCGCCGAC TACGGGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CACGAAATTG AGTCTTTATA CACCAAACTG GGCAAGGTTC CTCCTGCTGT CATCATTCCC
    CCAGCAGCCC CCCTTGCAGG GAGAAGGAGG CGACCCACCA AAAGCAAAGG GAGCAAGTCC
    AGTCGCAGCA GCTCTTTGGG GAATAAAAGC CCTGGACCAG GTACCCTGTC TGGCCAGAGT
    GCAGCTTCAG TCTTGCACCC TCAGCAGACC CTCCCCGCTC CTGGCAGCGT CCCGGAGACA
    GGGCCCAACC AGCTGTTACA GCCTGTGAAG CCATCTCCTT CCAGTGACAA CCTGTACTCG
    GCCTTTACCA GCGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCGCCAGG TCAAGGCTGT
    GCCAAGTTTA ACTGTGCGTC CGAGCAGGTG ACGTTCAAAG CTGGCGGCAG GAGGACGCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG CCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACCAACGCC GTTGGGGGAG CCGTGAGCAG CCAGGCCGCC
    CCGGCTCCAC CTCCTGCCGT GACGTCCAGC AGGAAGGGCA TGTTCACGGA CGACCTGCAC
    AAGCTGGTGG ACAACTGGGC CCGGGACGCC ATGAGTCTCT CAGGCAGGCG GGGAAGCAAA
    GGACACGTGA GCTATGAGGG CCCTGGCATG GCAAGGAAGT TCTCTGCGCC CGGTCAGCTG
    TGCGTCTCCA TGACCTCAAA CCTGGGCGGC TCTGCCCCCG GCTCTGCAGC GTCAGCTACC
    TCTCTAGGGC ACTTCCCCAA GTCCATGTGC CCCCCTCAGC AGTACGGTTT CCCTACCCCC
    CCGTTTGGCA CGCAGTGGAG CGGGACGGGT GGCCCGGCAC CCCAGCCGCT TAGCCAGTTC
    CAGCCTGTGG GAACAGCCTC CTTACAGAAT TTCAACATCA GCAACTTGCA GAAATCCATC
    AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234930
    Clone ID Related Accession (Same CDS sequence) XM_024992732.1
    Accession Version XM_024992732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCAA
    GGAGTGCAGC CAACAGCCCC CTCTCAGCAG GCGGTGCAGT ATCCGCTCCC TCAGACATCG
    GCCCCCAGCG AGGCCACGAT TGCACAGCCT GTGAGTCAGC CTCCACCTCC ACAGGCCTTG
    CCACACGTGT CCGCGGGAAA ACAGAGCACT CAGGTCGTCT CTCAGGTGGC TCCTCCAGAG
    CCAGCTCCCG GGGCTCAGCC ACAGCCTGCC CAGCCAGCCG CGGTGGTCTC TTCTATAGAC
    AGCGCACATT CAGATGTGGC TTCAGGCCTG AGTGATGGCA ACGAGAATGT CCCCTCATCC
    AGCGGGAGGC ACGAGGGCAG GACCACGAAG CGGCTGCATC GGAAGTCTAT AAGGAGCCGC
    TCTCGTCACG AGAAGCCTTC CCGCCCCAAA CTGAGAATTC TCAATGTTTC AAATAAGGGA
    GATCGGGTGG TAGAATGTCA GTTAGAGACT CACAATCGGA AGATGGTTAC ATTCAAATTT
    GATTTAGATG GTGACAACCC TGAGGAGATA GCCACAATTA TGGTGAACAA TGACTTTATT
    CTGGCACTGG AGAGAGATGC GTTTGTGGAG CAAGTGCGGG AGATCATTGA GAAAGCTGAC
    GAGATGCTCA GTGAGGATGT CAGCGTGGAG CCAGAGGGTG ACCAGAGCTT GGAGAGTCTC
    CATGGCAAGG ATGACTATGG CTTCTCAGGC ACCCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CATCTTCCTT GCCACAACAG ATAGGCATTC CTGCCAGTTC TTTAACTCAA
    GTTGTTCATT CTGCTGGAAG ACGGTTTATA GTAAGTCCTG TGCCAGAAAG TCGATTACGA
    GAATCAAAAC TTTTCACTGA AATTTCAGAT ACGGTTGCTG CTTCCACATC TGCTGGCCCT
    GGAATGAACT TGTCTCACTC TGCCTCATCC CTTAGTCTGC AGCAGGCCTT TTCTGAACTT
    AGACATGCTC GGATGGCAGA AGGGCCGAGT ACGGCCCCTC CCCACTTTGG TCAGACAGGC
    CCCGTGTTTC CAGCAGTGCC TCCCACCCTG AGCAGTGCCC CTGGAGCCCC AGCCGCAGCA
    GCCGCAGCCT CGGTGTCAGC CCCTGCCCCA AGCCACCCTC TTAGTGACGT GACCACATCG
    GTCATTCAGA CTGAGGTGAC AGTGGCCCCT GGAACAGGTG TCATGCCTCC CAGTGGTCTC
    CCTGTACCCC CTGTGTCTGA GTCCCTGGCA CCTTCCAGCG TGGCCCCCAG CGTCACCATC
    CCTGCAGTTC TCTCGGCACT ACCAGCGCCC CAGGCGGCAC AGGCTCCCAC CCCTGGCAGC
    GGGGCCTCCA GTGCAGGCAC TTTCCCCTCT GCACCCGCAT CCCTGACACC AGCCTCTGCT
    GTGGGCAGCG CTGTTGCCTC TGCTGCTCAG CTTCCGCCTG TGCCGTCTCA GCCCGCGGTA
    GGCAGCTCGA CCGGGGTGGC CACGCTGACA GCCGTGCCCG CTGCCGCTCT GGGCCCTAGC
    CTCACGGCAC TGCCGTCCCT TGCTCTCAGC AGCAGTACCT CTGCCCCAGC TCTGGCAGAG
    ACAGTGCTGG TCAGTGCCCA CGCACTGGAT AAGACGGCCC ATAGTAGCAC CACTGGCCTG
    GCTCTGTCCC TTCCTGCCAC CTCGTCCTCT CCCGCCCCTG GAGCAGGAGT GTCCAGTGCT
    GTTTCTCAGC CTACTGCTGC GGCTCACCCT TTGGTCCTCC CATCAGTCGT AACCTCTACT
    CCCGTACTCT CCCAGGCAGG ACCCATGTCA GCACCTCTAC TGCCCCAGGT ACCCGGTATT
    CCACCCCTGG TACAACCTGT TGTCAGCGTG CCTGCTGTGC AACAGACCCT GGTTCATAGT
    CAGCCTCAGC CCGCTCCACT CCCCAACCAG CCCCACACTC ACTGTCCCGA GATAGACGCC
    GACACTCAAC CCAAAGCTCC CGGGATCGAC GACATCAAGA CTTTGGAGGA AAAGCTGCGA
    TCTCTCTTTA GTGAGCACAG TTCTTCTGGA GCCCAACACG CCCCCGTCTC TCTGGAGACC
    TCGCTCACTG TAGAGACCGC TGTCACAGCA GGCCTCCCAA CCACTGCTGT TGCACCAAGC
    AAGCCTCTGA CTTCCACTGC CAGTACTTGC TTGCCACCAA CCAGTCTGCC CTTAGGAGCA
    GCTGGCTTGT CAGTTATACC AATGGTCACT CCTGGTCAAG TTTCCACCCC AGTCAGCTCT
    GCGCCAGGAG TGAAACTTGA AACTGCTCCA GCAAAGCCAC CTTTAACTAA GCCTCCAGTG
    CTGCCCATGG GCACTGAACT TCCAGCTGGT ACTCCACCCA GCGAGCAGCT GCCACCTTTT
    CCAGGACCTT CACTAACTCA GCCCCCGCAG CCCCTGGGAG ACCTGGACGC TCAGCTGAGA
    AGAACCCTCA GTCCCGAGAG TGGCATGCTG ACGTCTGCCG TTGGACCCGT GTCTGTGGTG
    GCTCCAACAG CAGTTGTGGA GACCGAAGCC CAACTTCCAA AGGATGTTCT TTCAGTCTCT
    CAAGTCACAG AAGCTCCTGT CCTTGCGCCC ACTTCCACAG CTGGTGTTCT GAAGATGGGG
    CGATTTCAGG TCTCAGTTGC AATGGATGAC CCCCAGAAGG AGGGTAAAAG TAAGTCGGAG
    GATGCCAAGT CTGTCCACTT CGCATCCAGC ACGTCAGAGT CCTCGGTGCT GTCAAGCAGC
    AGCCCCGAGA GCACCGTGGT GAAAGCAGAG CCCAACGGGC TGGCCGTCCG GGGCGCCTCT
    GCAGACATGC CTGACAGCGC CCACCAGCCA CCTGCCTCAG AAGCTGGGCA GCCTAGCAAG
    GTGGGCCGTT TTCAGGTGAC CACCACAGCG AGCAGAGTGG GTCGCTTCTC TGTGTCCAGA
    ACTGAAGACA AGATGGCTGA GGTGAAGGGA GAGGAGCCAG TGGTCTCTCC TTTCATGGAC
    TCAGAACACG ACATTCCTCC CACCGTGACG CCAAAGAAAG AGAAACCCGA ACTGTCTGAG
    CCTTCGCATC TGAACGGGCC GTCTTCCGAC CTGGAGGCTG CCTTCCTCAG CCGGGACGTG
    GATGCTGGCT CAGGGAGCCC TCACTCCCCT CCGCAGCTGA GCTCCAAGAG CCTTCCTGCC
    CAGAGCCTCA GTCAGAGCCT CAGCAATTCC TTCAACTCTT CCTACATGAG CAGCGACAAT
    GAGTCAGACA TTGAAGACGA GGACTTAAAG TTAGAGCTTC GCCGACTACG GGAAAAACAT
    CTTAAAGAGA TTCAGGACCT GCAGAGTCGC CAGAAGCACG AAATTGAGTC TTTATACACC
    AAACTGGGCA AGGTTCCTCC TGCTGTCATC ATTCCCCCAG CAGCCCCCCT TGCAGGGAGA
    AGGAGGCGAC CCACCAAAAG CAAAGGGAGC AAGTCCAGTC GCAGCAGCTC TTTGGGGAAT
    AAAAGCCCTG GACCAGGTAC CCTGTCTGGC CAGAGTGCAG CTTCAGTCTT GCACCCTCAG
    CAGACCCTCC CCGCTCCTGG CAGCGTCCCG GAGACAGGGC CCAACCAGCT GTTACAGCCT
    GTGAAGCCAT CTCCTTCCAG TGACAACCTG TACTCGGCCT TTACCAGCGA TGGTGCCATT
    TCAGTACCAA GCCTTTCTGC GCCAGGTCAA GGGACCAGCA GCACCAACGC CGTTGGGGGA
    GCCGTGAGCA GCCAGGCCGC CCCGGCTCCA CCTCCTGCCG TGACGTCCAG CAGGAAGGGC
    ATGTTCACGG ACGACCTGCA CAAGCTGGTG GACAACTGGG CCCGGGACGC CATGAGTCTC
    TCAGGCAGGC GGGGAAGCAA AGGACACGTG AGCTATGAGG GCCCTGGCAT GGCAAGGAAG
    TTCTCTGCGC CCGGTCAGCT GTGCGTCTCC ATGACCTCAA ACCTGGGCGG CTCTGCCCCC
    GGCTCTGCAG CGTCAGCTAC CTCTCTAGGG CACTTCCCCA AGTCCATGTG CCCCCCTCAG
    CAGTACGGTT TCCCTACCCC CCCGTTTGGC ACGCAGTGGA GCGGGACGGG TGGCCCGGCA
    CCCCAGCCGC TTAGCCAGTT CCAGCCTGTG GGAACAGCCT CCTTACAGAA TTTCAACATC
    AGCAACTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848500.1
    CDS127..6423
    Translation

    Target ORF information:

    RefSeq Version XM_024992732.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCAA
    GGAGTGCAGC CAACAGCCCC CTCTCAGCAG GCGGTGCAGT ATCCGCTCCC TCAGACATCG
    GCCCCCAGCG AGGCCACGAT TGCACAGCCT GTGAGTCAGC CTCCACCTCC ACAGGCCTTG
    CCACACGTGT CCGCGGGAAA ACAGAGCACT CAGGTCGTCT CTCAGGTGGC TCCTCCAGAG
    CCAGCTCCCG GGGCTCAGCC ACAGCCTGCC CAGCCAGCCG CGGTGGTCTC TTCTATAGAC
    AGCGCACATT CAGATGTGGC TTCAGGCCTG AGTGATGGCA ACGAGAATGT CCCCTCATCC
    AGCGGGAGGC ACGAGGGCAG GACCACGAAG CGGCTGCATC GGAAGTCTAT AAGGAGCCGC
    TCTCGTCACG AGAAGCCTTC CCGCCCCAAA CTGAGAATTC TCAATGTTTC AAATAAGGGA
    GATCGGGTGG TAGAATGTCA GTTAGAGACT CACAATCGGA AGATGGTTAC ATTCAAATTT
    GATTTAGATG GTGACAACCC TGAGGAGATA GCCACAATTA TGGTGAACAA TGACTTTATT
    CTGGCACTGG AGAGAGATGC GTTTGTGGAG CAAGTGCGGG AGATCATTGA GAAAGCTGAC
    GAGATGCTCA GTGAGGATGT CAGCGTGGAG CCAGAGGGTG ACCAGAGCTT GGAGAGTCTC
    CATGGCAAGG ATGACTATGG CTTCTCAGGC ACCCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CATCTTCCTT GCCACAACAG ATAGGCATTC CTGCCAGTTC TTTAACTCAA
    GTTGTTCATT CTGCTGGAAG ACGGTTTATA GTAAGTCCTG TGCCAGAAAG TCGATTACGA
    GAATCAAAAC TTTTCACTGA AATTTCAGAT ACGGTTGCTG CTTCCACATC TGCTGGCCCT
    GGAATGAACT TGTCTCACTC TGCCTCATCC CTTAGTCTGC AGCAGGCCTT TTCTGAACTT
    AGACATGCTC GGATGGCAGA AGGGCCGAGT ACGGCCCCTC CCCACTTTGG TCAGACAGGC
    CCCGTGTTTC CAGCAGTGCC TCCCACCCTG AGCAGTGCCC CTGGAGCCCC AGCCGCAGCA
    GCCGCAGCCT CGGTGTCAGC CCCTGCCCCA AGCCACCCTC TTAGTGACGT GACCACATCG
    GTCATTCAGA CTGAGGTGAC AGTGGCCCCT GGAACAGGTG TCATGCCTCC CAGTGGTCTC
    CCTGTACCCC CTGTGTCTGA GTCCCTGGCA CCTTCCAGCG TGGCCCCCAG CGTCACCATC
    CCTGCAGTTC TCTCGGCACT ACCAGCGCCC CAGGCGGCAC AGGCTCCCAC CCCTGGCAGC
    GGGGCCTCCA GTGCAGGCAC TTTCCCCTCT GCACCCGCAT CCCTGACACC AGCCTCTGCT
    GTGGGCAGCG CTGTTGCCTC TGCTGCTCAG CTTCCGCCTG TGCCGTCTCA GCCCGCGGTA
    GGCAGCTCGA CCGGGGTGGC CACGCTGACA GCCGTGCCCG CTGCCGCTCT GGGCCCTAGC
    CTCACGGCAC TGCCGTCCCT TGCTCTCAGC AGCAGTACCT CTGCCCCAGC TCTGGCAGAG
    ACAGTGCTGG TCAGTGCCCA CGCACTGGAT AAGACGGCCC ATAGTAGCAC CACTGGCCTG
    GCTCTGTCCC TTCCTGCCAC CTCGTCCTCT CCCGCCCCTG GAGCAGGAGT GTCCAGTGCT
    GTTTCTCAGC CTACTGCTGC GGCTCACCCT TTGGTCCTCC CATCAGTCGT AACCTCTACT
    CCCGTACTCT CCCAGGCAGG ACCCATGTCA GCACCTCTAC TGCCCCAGGT ACCCGGTATT
    CCACCCCTGG TACAACCTGT TGTCAGCGTG CCTGCTGTGC AACAGACCCT GGTTCATAGT
    CAGCCTCAGC CCGCTCCACT CCCCAACCAG CCCCACACTC ACTGTCCCGA GATAGACGCC
    GACACTCAAC CCAAAGCTCC CGGGATCGAC GACATCAAGA CTTTGGAGGA AAAGCTGCGA
    TCTCTCTTTA GTGAGCACAG TTCTTCTGGA GCCCAACACG CCCCCGTCTC TCTGGAGACC
    TCGCTCACTG TAGAGACCGC TGTCACAGCA GGCCTCCCAA CCACTGCTGT TGCACCAAGC
    AAGCCTCTGA CTTCCACTGC CAGTACTTGC TTGCCACCAA CCAGTCTGCC CTTAGGAGCA
    GCTGGCTTGT CAGTTATACC AATGGTCACT CCTGGTCAAG TTTCCACCCC AGTCAGCTCT
    GCGCCAGGAG TGAAACTTGA AACTGCTCCA GCAAAGCCAC CTTTAACTAA GCCTCCAGTG
    CTGCCCATGG GCACTGAACT TCCAGCTGGT ACTCCACCCA GCGAGCAGCT GCCACCTTTT
    CCAGGACCTT CACTAACTCA GCCCCCGCAG CCCCTGGGAG ACCTGGACGC TCAGCTGAGA
    AGAACCCTCA GTCCCGAGAG TGGCATGCTG ACGTCTGCCG TTGGACCCGT GTCTGTGGTG
    GCTCCAACAG CAGTTGTGGA GACCGAAGCC CAACTTCCAA AGGATGTTCT TTCAGTCTCT
    CAAGTCACAG AAGCTCCTGT CCTTGCGCCC ACTTCCACAG CTGGTGTTCT GAAGATGGGG
    CGATTTCAGG TCTCAGTTGC AATGGATGAC CCCCAGAAGG AGGGTAAAAG TAAGTCGGAG
    GATGCCAAGT CTGTCCACTT CGCATCCAGC ACGTCAGAGT CCTCGGTGCT GTCAAGCAGC
    AGCCCCGAGA GCACCGTGGT GAAAGCAGAG CCCAACGGGC TGGCCGTCCG GGGCGCCTCT
    GCAGACATGC CTGACAGCGC CCACCAGCCA CCTGCCTCAG AAGCTGGGCA GCCTAGCAAG
    GTGGGCCGTT TTCAGGTGAC CACCACAGCG AGCAGAGTGG GTCGCTTCTC TGTGTCCAGA
    ACTGAAGACA AGATGGCTGA GGTGAAGGGA GAGGAGCCAG TGGTCTCTCC TTTCATGGAC
    TCAGAACACG ACATTCCTCC CACCGTGACG CCAAAGAAAG AGAAACCCGA ACTGTCTGAG
    CCTTCGCATC TGAACGGGCC GTCTTCCGAC CTGGAGGCTG CCTTCCTCAG CCGGGACGTG
    GATGCTGGCT CAGGGAGCCC TCACTCCCCT CCGCAGCTGA GCTCCAAGAG CCTTCCTGCC
    CAGAGCCTCA GTCAGAGCCT CAGCAATTCC TTCAACTCTT CCTACATGAG CAGCGACAAT
    GAGTCAGACA TTGAAGACGA GGACTTAAAG TTAGAGCTTC GCCGACTACG GGAAAAACAT
    CTTAAAGAGA TTCAGGACCT GCAGAGTCGC CAGAAGCACG AAATTGAGTC TTTATACACC
    AAACTGGGCA AGGTTCCTCC TGCTGTCATC ATTCCCCCAG CAGCCCCCCT TGCAGGGAGA
    AGGAGGCGAC CCACCAAAAG CAAAGGGAGC AAGTCCAGTC GCAGCAGCTC TTTGGGGAAT
    AAAAGCCCTG GACCAGGTAC CCTGTCTGGC CAGAGTGCAG CTTCAGTCTT GCACCCTCAG
    CAGACCCTCC CCGCTCCTGG CAGCGTCCCG GAGACAGGGC CCAACCAGCT GTTACAGCCT
    GTGAAGCCAT CTCCTTCCAG TGACAACCTG TACTCGGCCT TTACCAGCGA TGGTGCCATT
    TCAGTACCAA GCCTTTCTGC GCCAGGTCAA GGGACCAGCA GCACCAACGC CGTTGGGGGA
    GCCGTGAGCA GCCAGGCCGC CCCGGCTCCA CCTCCTGCCG TGACGTCCAG CAGGAAGGGC
    ATGTTCACGG ACGACCTGCA CAAGCTGGTG GACAACTGGG CCCGGGACGC CATGAGTCTC
    TCAGGCAGGC GGGGAAGCAA AGGACACGTG AGCTATGAGG GCCCTGGCAT GGCAAGGAAG
    TTCTCTGCGC CCGGTCAGCT GTGCGTCTCC ATGACCTCAA ACCTGGGCGG CTCTGCCCCC
    GGCTCTGCAG CGTCAGCTAC CTCTCTAGGG CACTTCCCCA AGTCCATGTG CCCCCCTCAG
    CAGTACGGTT TCCCTACCCC CCCGTTTGGC ACGCAGTGGA GCGGGACGGG TGGCCCGGCA
    CCCCAGCCGC TTAGCCAGTT CCAGCCTGTG GGAACAGCCT CCTTACAGAA TTTCAACATC
    AGCAACTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89985
    Clone ID Related Accession (Same CDS sequence) XM_015471356.1 , XM_015463540.1
    Accession Version XM_015471356.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTGCCCAT GCCTAATTCA AGTCTTGGGA ATGGGACTCT GCGACTGGTT TAGGCCAGTC 
    GTGATTCCTT TCCTTGGACT GGAGGTAGGA CCTGCTTCTC TTCTGTCGCA AGGTGGATGC
    TTGAACCAAG ATCGAAAGTT AACAAAGTCT GAGAGGCAGC GATTTAAAGA AGAGGCTGAA
    ATGTTAAAGG GTCTTCAGCA TCCCAATATT GTTCGATTCT ATGATTCCTG GGAATCCACA
    GTAAAGGGAA AGAAGTGCAT TGTGTTGGTG ACTGAACTGA TGACATCTGG AACTCTTAAA
    ACGTACCTGA AAAGGTTTAA GGTGATGAAG ATCAAAGTTT TGAGAAGCTG GTGCCGTCAG
    ATTCTTAAAG GACTTCAGTT TCTTCATACT AGAACTCCAC CTATTATTCA CCGGGATCTT
    AAGTGTGACA ACATCTTTAT CACTGGCCCC ACAGGCTCAG TCAAGATTGG AGACCTAGGT
    CTGGCCACCC TGAAGCGGGC TTCTTTTGCC AAGAGTGTGA TAGGTACCCC AGAGTTCATG
    GCCCCTGAGA TGTATGAAGA GAAGTACGAT GAATCCGTTG ATGTTTATGC TTTTGGGATG
    TGCATGCTCG AGATGGCCAC GTCTGAATAC CCGTACTCAG AGTGTCAGAA CGCTGCACAG
    ATCTACCGTC GTGTGACCAG CGGGGTGAAG CCAGCCAGTT TTGACAAAGT AGCAATTCCT
    GAAGTGAAGG AAATTATTGA AGGATGCATA CGGCAAAACA AAGATGAAAG ATACTCTATC
    AAAGACCTTT TGAACCATGC CTTCTTCCAG GAGGAAACAG GGGTACGGGT AGAATTAGCA
    GAAGAAGATG ATGGAGAAAA GATAGCCATT AAATTATGGC TACGTATTGA AGATATTAAG
    AAACTAAAGG GGAAATACAA AGACAATGAA GCTATCGAGT TTTCCTTTGA CTTGGAGAAA
    GATGTGCCAG AAGACGTTGC TCAAGAAATG GTAGATTCTG GGTATGTCTG TGAAGGTGAT
    CACAAGACCA TGGCAAAAGC CATCAAAGAT AGAGTGTCGT TAATTAAGAG AAAACGAGAA
    CAGCGTCAAT TAGTGCGGGA GGAGCAAGAA AAAAGAAAGC AGGAGGAGAG CAGCTTCAAG
    CAGCAGGTGG AGCAGCAGCC CAGCACTGCC CAGGCCGGAG TCCAGCAGCC CCCTGCCACC
    AGCACTGGTG GACCCGCTGC CTCTGCTGCG TCTGCGTCCG TCTCCACGCA GGTAGAGCCA
    GAAGAGCCTG AGGCCGACCA GCACCAGCAG CTGCAGTACC AGCAGCCTGG TGTGTCTGTG
    ATATCTGACG GGACGGTTGA CAGTGGTCAG GGGTCTTCTG TCTTCACGGA ATCTCGAGTC
    AGCAGCCAAC AGACAGTTTC GTACGGGTCC CAGCATGAGC AGGCACCTTC CACTGGCGCC
    CTCCCAGGGC ACACAGCATC TGTTGTCCAA ACGCAGGCTC AGCCCCATGC GGTATATCCA
    CCCTCAAGTT TGCCTCAGTC CATGGCGCAT CCGCGTGGGG GGACCCCAAC ATACCCAGAA
    TCACAGATAT TTTTCCCAAC TATTCATGAA CGTCCAGTTT CTTTTTCACC ACCTCCCACC
    TGTCCACCGA AGGTGGCCAT TGCCCAGCGG CGTAAGAGCA CCTCCTTCTT GGAAGCCCAA
    ACTCACCAGT TCCAACCCCT GCTGAGGACT GTCGGGCAGA GTCTTCTTCC ACCTGGTGGC
    AGCCCACCTA ACTGGACTCC AGAGACTGTA GTTATGTGGG GCACTGCAGC CAGTAGAGTT
    GCTGGAGAGC TGTGTGAGGT GCAGGCCCAT CCTGTGTTCG AGCCAGCTCC AGTTTACAGT
    GACTACAGAG CTGGACTGAT GCTTCCAGAA GAAGCTCACT ACTTCCTCCC TCCAGAAGCA
    GTGTATCTAG CCGGGGTACA TTACCAGGCC CAGGTGGCAG AACAGTTTGA GGGTTTTCCA
    TACAGCTCAC CAGTCCTGTC AAGTCCTATG AAGCAGACAC CTGAGCAGAA GCCAGTGCAC
    GGGGGCCCCA CCTCCAGTTC TGTCTTTGAA TTTCCATCGG GACAGGCTTT CCTGGTAGGA
    CACCTCCAGA ATGTAAGATT AGATTCGGGA CTGAGCCCAG GCTCTCCCCA GTCTAGCGTT
    TCTGCACCTA TCAGTACAGA TGCCACACGC TTGAAGTTTC ACCCTGTCTT TGTTCCTCAT
    TCTGCACCTG CTGTGTTAAC TCATAACAAC GAGAGCAGAA GCAGTTGTGT GTTTGAGTTT
    CACGTTCATA CTCCAAGCTC CTCTTCAGGA GAAGGAGGGG GAGGAGTTTT ACCTCAGCGC
    ATTTACCGAA ATCGGCAGGT TGCTGTGGAC TCGAACCAGG AAGAACCACC TCTTCAACCT
    GCAGGACTAC ACAGCCGCCT GCAGCCTGTG ACTGAAGAAC AGCAGATGCC CCAGGCCCCA
    GAGTTGACCA TCTCTGTGGT GGAGCCCACC GGACAGACCT GGCCCATAGG AAGCCCAGAG
    TACTCCAGTG ATTCCTCCCA GGTCACTTCT TCAGACCCCA GTGAATTTCA GTCACCTCCC
    CCTACAGGGG GAGCAGCTGC TCCTTTTGGC TCTGACGTCT CATTACCCTT TATCCGCGTG
    CCTCAGACAG TGTTACAAGA ATCCCCACTC TTCTTCTGTT TCCCCCAAGG AGCCACCTCT
    CCGCAGGTTT TATCCGCCTC ATTTTCTTCA GGAGGATCTG CACTCCATCC ACAGGCACAG
    GGGCAGAGCC AGGGTCAGCC ATCTTCAAGT AGTTTAACAG GGGTTCCATC CTCCCAACCC
    ATGCAGCATT CTCAGCAGCA GCAAGGAGTG CAGCCAACAG CCCCCTCTCA GCAGGCGGTG
    CAGTATCCGC TCCCTCAGAC ATCGGCCCCC AGCGAGGCCA CGATTGCACA GCCTGTGAGT
    CAGCCTCCAC CTCCACAGGC CTTGCCACAC GTGTCCGCGG GAAAACAGCT TCCAGTTTCC
    CAGCCAGTAC CAACTCTCCA AGGCGAACCT CAGATCCCAG TTGCAACACA GCCCTCAGTC
    GTTCCCGTCC ATGCTGGTGC TCACTTCCTT CCTGTGGGAC AGCCACTCCC ACCCTCTTTA
    CTGCCCCAGT ACCCCGTCTC TCAGCTGCCC CCCATCTCTC AGCTCCCCTC TGCTCCTCCT
    CACGTGTCTG TGGCTCAGCC AGGCTTCCCA CCCCTGCCGG TCACGATGGC AGCTGGCGTT
    AGTCAGCCTC TGCTCACCTT GGCTTCATCT GCTGCGGCGG CGGCCGTCCC CGGGGCACCC
    ACTGTGGTCC CTAGTCAGCT TCCTGCCCTC CTGCAGCCTG TGACCCAGCT GCCGAGTCAG
    GCTCACCCGC AGCTCCTGCA GCCAGCAGTT CAGTCCATGG GGATCCCCGC CAGCCTTGGA
    CAGACTGCCG AGGTGCCACT TCCCCCTGGA GATATTCTCT ACCAGGGCTT CCCACCTCGA
    CTGCCATCGC AGTACCCAGG AGACTCAAAC ATTGCCCCCT CCTCCAGCGC AGCTTCTGTG
    TGCCTGCCTT CTGCAGTCCT GTCCCCTCCC ATGCCGACAG AGGCGCTGGC TGCCCCGGGT
    TACTTTCCTG CAATGGTGCA GCCCTATGTG GAATCCAGTC TCTTGGTTCC TGTGGGCAGT
    GTAGGAGGAC AGGTCCAGGT GTCCCAGCCG GCAGTGAGCT TAGCACAAGC CCCCACCACA
    TCCTCCCAGC AAGCAGCTCT GGAGAGCACT CAGGTCGTCT CTCAGGTGGC TCCTCCAGAG
    CCAGCTCCCG GGGCTCAGCC ACAGCCTGCC CAGCCAGCCG CGGTGGTCTC TTCTATAGAC
    AGCGCACATT CAGATGTGGC TTCAGGCCTG AGTGATGGCA ACGAGAATGT CCCCTCATCC
    AGCGGGAGGC ACGAGGGCAG GACCACGAAG CGGCTGCATC GGAAGTCTAT AAGGAGCCGC
    TCTCGTCACG AGAAGCCTTC CCGCCCCAAA CTGAGAATTC TCAATGTTTC AAATAAGGGA
    GATCGGGTGG TAGAATGTCA GTTAGAGACT CACAATCGGA AGATGGTTAC ATTCAAATTT
    GATTTAGATG GTGACAACCC TGAGGAGATA GCCACAATTA TGGTGAACAA TGACTTTATT
    CTGGCACTGG AGAGAGATGC GTTTGTGGAG CAAGTGCGGG AGATCATTGA GAAAGCTGAC
    GAGATGCTCA GTGAGGATGT CAGCGTGGAG CCAGAGGGTG ACCAGAGCTT GGAGAGTCTC
    CATGGCAAGG ATGACTATGG CTTCTCAGGC ACCCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CATCTTCCTT GCCACAACAG ATAGCAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGC CCTTGCAACC AGCTCTGTAT GTGCCCTCCA
    GCAGAGCCGA AATCAGGGAC CAGCAGCACC AACGCCGTTG GGGGAGCCGT GAGCAGCCAG
    GCCGCCCCGG CTCCACCTCC TGCCGTGACG TCCAGCAGGA AGGGCATGTT CACGGACGAC
    CTGCACAAGC TGGTGGACAA CTGGGCCCGG GACGCCATGA GTCTCTCAGG CAGGCGGGGA
    AGCAAAGGAC ACGTGAGCTA TGAGGGCCCT GGCATGGCAA GGAAGTTCTC TGCGCCCGGT
    CAGCTGTGCG TCTCCATGAC CTCAAACCTG GGCGGCTCTG CCCCCGGCTC TGCAGCGTCA
    GCTACCTCTC TAGGGCACTT CCCCAAGTCC ATGTGCCCCC CTCAGCAGTA CGGTTTCCCT
    ACCCCCCCGT TTGGCACGCA GTGGAGCGGG ACGGGTGGCC CGGCACCCCA GCCGCTTAGC
    CAGTTCCAGC CTGTGGGAAC AGCCTCCTTA CAGAATTTCA ACATCAGCAA CTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326842.1
    CDS6..7907
    Translation

    Target ORF information:

    RefSeq Version XM_015471356.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471356.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTGCCCAT GCCTAATTCA AGTCTTGGGA ATGGGACTCT GCGACTGGTT TAGGCCAGTC 
    GTGATTCCTT TCCTTGGACT GGAGGTAGGA CCTGCTTCTC TTCTGTCGCA AGGTGGATGC
    TTGAACCAAG ATCGAAAGTT AACAAAGTCT GAGAGGCAGC GATTTAAAGA AGAGGCTGAA
    ATGTTAAAGG GTCTTCAGCA TCCCAATATT GTTCGATTCT ATGATTCCTG GGAATCCACA
    GTAAAGGGAA AGAAGTGCAT TGTGTTGGTG ACTGAACTGA TGACATCTGG AACTCTTAAA
    ACGTACCTGA AAAGGTTTAA GGTGATGAAG ATCAAAGTTT TGAGAAGCTG GTGCCGTCAG
    ATTCTTAAAG GACTTCAGTT TCTTCATACT AGAACTCCAC CTATTATTCA CCGGGATCTT
    AAGTGTGACA ACATCTTTAT CACTGGCCCC ACAGGCTCAG TCAAGATTGG AGACCTAGGT
    CTGGCCACCC TGAAGCGGGC TTCTTTTGCC AAGAGTGTGA TAGGTACCCC AGAGTTCATG
    GCCCCTGAGA TGTATGAAGA GAAGTACGAT GAATCCGTTG ATGTTTATGC TTTTGGGATG
    TGCATGCTCG AGATGGCCAC GTCTGAATAC CCGTACTCAG AGTGTCAGAA CGCTGCACAG
    ATCTACCGTC GTGTGACCAG CGGGGTGAAG CCAGCCAGTT TTGACAAAGT AGCAATTCCT
    GAAGTGAAGG AAATTATTGA AGGATGCATA CGGCAAAACA AAGATGAAAG ATACTCTATC
    AAAGACCTTT TGAACCATGC CTTCTTCCAG GAGGAAACAG GGGTACGGGT AGAATTAGCA
    GAAGAAGATG ATGGAGAAAA GATAGCCATT AAATTATGGC TACGTATTGA AGATATTAAG
    AAACTAAAGG GGAAATACAA AGACAATGAA GCTATCGAGT TTTCCTTTGA CTTGGAGAAA
    GATGTGCCAG AAGACGTTGC TCAAGAAATG GTAGATTCTG GGTATGTCTG TGAAGGTGAT
    CACAAGACCA TGGCAAAAGC CATCAAAGAT AGAGTGTCGT TAATTAAGAG AAAACGAGAA
    CAGCGTCAAT TAGTGCGGGA GGAGCAAGAA AAAAGAAAGC AGGAGGAGAG CAGCTTCAAG
    CAGCAGGTGG AGCAGCAGCC CAGCACTGCC CAGGCCGGAG TCCAGCAGCC CCCTGCCACC
    AGCACTGGTG GACCCGCTGC CTCTGCTGCG TCTGCGTCCG TCTCCACGCA GGTAGAGCCA
    GAAGAGCCTG AGGCCGACCA GCACCAGCAG CTGCAGTACC AGCAGCCTGG TGTGTCTGTG
    ATATCTGACG GGACGGTTGA CAGTGGTCAG GGGTCTTCTG TCTTCACGGA ATCTCGAGTC
    AGCAGCCAAC AGACAGTTTC GTACGGGTCC CAGCATGAGC AGGCACCTTC CACTGGCGCC
    CTCCCAGGGC ACACAGCATC TGTTGTCCAA ACGCAGGCTC AGCCCCATGC GGTATATCCA
    CCCTCAAGTT TGCCTCAGTC CATGGCGCAT CCGCGTGGGG GGACCCCAAC ATACCCAGAA
    TCACAGATAT TTTTCCCAAC TATTCATGAA CGTCCAGTTT CTTTTTCACC ACCTCCCACC
    TGTCCACCGA AGGTGGCCAT TGCCCAGCGG CGTAAGAGCA CCTCCTTCTT GGAAGCCCAA
    ACTCACCAGT TCCAACCCCT GCTGAGGACT GTCGGGCAGA GTCTTCTTCC ACCTGGTGGC
    AGCCCACCTA ACTGGACTCC AGAGACTGTA GTTATGTGGG GCACTGCAGC CAGTAGAGTT
    GCTGGAGAGC TGTGTGAGGT GCAGGCCCAT CCTGTGTTCG AGCCAGCTCC AGTTTACAGT
    GACTACAGAG CTGGACTGAT GCTTCCAGAA GAAGCTCACT ACTTCCTCCC TCCAGAAGCA
    GTGTATCTAG CCGGGGTACA TTACCAGGCC CAGGTGGCAG AACAGTTTGA GGGTTTTCCA
    TACAGCTCAC CAGTCCTGTC AAGTCCTATG AAGCAGACAC CTGAGCAGAA GCCAGTGCAC
    GGGGGCCCCA CCTCCAGTTC TGTCTTTGAA TTTCCATCGG GACAGGCTTT CCTGGTAGGA
    CACCTCCAGA ATGTAAGATT AGATTCGGGA CTGAGCCCAG GCTCTCCCCA GTCTAGCGTT
    TCTGCACCTA TCAGTACAGA TGCCACACGC TTGAAGTTTC ACCCTGTCTT TGTTCCTCAT
    TCTGCACCTG CTGTGTTAAC TCATAACAAC GAGAGCAGAA GCAGTTGTGT GTTTGAGTTT
    CACGTTCATA CTCCAAGCTC CTCTTCAGGA GAAGGAGGGG GAGGAGTTTT ACCTCAGCGC
    ATTTACCGAA ATCGGCAGGT TGCTGTGGAC TCGAACCAGG AAGAACCACC TCTTCAACCT
    GCAGGACTAC ACAGCCGCCT GCAGCCTGTG ACTGAAGAAC AGCAGATGCC CCAGGCCCCA
    GAGTTGACCA TCTCTGTGGT GGAGCCCACC GGACAGACCT GGCCCATAGG AAGCCCAGAG
    TACTCCAGTG ATTCCTCCCA GGTCACTTCT TCAGACCCCA GTGAATTTCA GTCACCTCCC
    CCTACAGGGG GAGCAGCTGC TCCTTTTGGC TCTGACGTCT CATTACCCTT TATCCGCGTG
    CCTCAGACAG TGTTACAAGA ATCCCCACTC TTCTTCTGTT TCCCCCAAGG AGCCACCTCT
    CCGCAGGTTT TATCCGCCTC ATTTTCTTCA GGAGGATCTG CACTCCATCC ACAGGCACAG
    GGGCAGAGCC AGGGTCAGCC ATCTTCAAGT AGTTTAACAG GGGTTCCATC CTCCCAACCC
    ATGCAGCATT CTCAGCAGCA GCAAGGAGTG CAGCCAACAG CCCCCTCTCA GCAGGCGGTG
    CAGTATCCGC TCCCTCAGAC ATCGGCCCCC AGCGAGGCCA CGATTGCACA GCCTGTGAGT
    CAGCCTCCAC CTCCACAGGC CTTGCCACAC GTGTCCGCGG GAAAACAGCT TCCAGTTTCC
    CAGCCAGTAC CAACTCTCCA AGGCGAACCT CAGATCCCAG TTGCAACACA GCCCTCAGTC
    GTTCCCGTCC ATGCTGGTGC TCACTTCCTT CCTGTGGGAC AGCCACTCCC ACCCTCTTTA
    CTGCCCCAGT ACCCCGTCTC TCAGCTGCCC CCCATCTCTC AGCTCCCCTC TGCTCCTCCT
    CACGTGTCTG TGGCTCAGCC AGGCTTCCCA CCCCTGCCGG TCACGATGGC AGCTGGCGTT
    AGTCAGCCTC TGCTCACCTT GGCTTCATCT GCTGCGGCGG CGGCCGTCCC CGGGGCACCC
    ACTGTGGTCC CTAGTCAGCT TCCTGCCCTC CTGCAGCCTG TGACCCAGCT GCCGAGTCAG
    GCTCACCCGC AGCTCCTGCA GCCAGCAGTT CAGTCCATGG GGATCCCCGC CAGCCTTGGA
    CAGACTGCCG AGGTGCCACT TCCCCCTGGA GATATTCTCT ACCAGGGCTT CCCACCTCGA
    CTGCCATCGC AGTACCCAGG AGACTCAAAC ATTGCCCCCT CCTCCAGCGC AGCTTCTGTG
    TGCCTGCCTT CTGCAGTCCT GTCCCCTCCC ATGCCGACAG AGGCGCTGGC TGCCCCGGGT
    TACTTTCCTG CAATGGTGCA GCCCTATGTG GAATCCAGTC TCTTGGTTCC TGTGGGCAGT
    GTAGGAGGAC AGGTCCAGGT GTCCCAGCCG GCAGTGAGCT TAGCACAAGC CCCCACCACA
    TCCTCCCAGC AAGCAGCTCT GGAGAGCACT CAGGTCGTCT CTCAGGTGGC TCCTCCAGAG
    CCAGCTCCCG GGGCTCAGCC ACAGCCTGCC CAGCCAGCCG CGGTGGTCTC TTCTATAGAC
    AGCGCACATT CAGATGTGGC TTCAGGCCTG AGTGATGGCA ACGAGAATGT CCCCTCATCC
    AGCGGGAGGC ACGAGGGCAG GACCACGAAG CGGCTGCATC GGAAGTCTAT AAGGAGCCGC
    TCTCGTCACG AGAAGCCTTC CCGCCCCAAA CTGAGAATTC TCAATGTTTC AAATAAGGGA
    GATCGGGTGG TAGAATGTCA GTTAGAGACT CACAATCGGA AGATGGTTAC ATTCAAATTT
    GATTTAGATG GTGACAACCC TGAGGAGATA GCCACAATTA TGGTGAACAA TGACTTTATT
    CTGGCACTGG AGAGAGATGC GTTTGTGGAG CAAGTGCGGG AGATCATTGA GAAAGCTGAC
    GAGATGCTCA GTGAGGATGT CAGCGTGGAG CCAGAGGGTG ACCAGAGCTT GGAGAGTCTC
    CATGGCAAGG ATGACTATGG CTTCTCAGGC ACCCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CATCTTCCTT GCCACAACAG ATAGCAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGC CCTTGCAACC AGCTCTGTAT GTGCCCTCCA
    GCAGAGCCGA AATCAGGGAC CAGCAGCACC AACGCCGTTG GGGGAGCCGT GAGCAGCCAG
    GCCGCCCCGG CTCCACCTCC TGCCGTGACG TCCAGCAGGA AGGGCATGTT CACGGACGAC
    CTGCACAAGC TGGTGGACAA CTGGGCCCGG GACGCCATGA GTCTCTCAGG CAGGCGGGGA
    AGCAAAGGAC ACGTGAGCTA TGAGGGCCCT GGCATGGCAA GGAAGTTCTC TGCGCCCGGT
    CAGCTGTGCG TCTCCATGAC CTCAAACCTG GGCGGCTCTG CCCCCGGCTC TGCAGCGTCA
    GCTACCTCTC TAGGGCACTT CCCCAAGTCC ATGTGCCCCC CTCAGCAGTA CGGTTTCCCT
    ACCCCCCCGT TTGGCACGCA GTGGAGCGGG ACGGGTGGCC CGGCACCCCA GCCGCTTAGC
    CAGTTCCAGC CTGTGGGAAC AGCCTCCTTA CAGAATTTCA ACATCAGCAA CTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89985
    Clone ID Related Accession (Same CDS sequence) XM_015471356.1 , XM_015463540.1
    Accession Version XM_015463540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTGCCCAT GCCTAATTCA AGTCTTGGGA ATGGGACTCT GCGACTGGTT TAGGCCAGTC 
    GTGATTCCTT TCCTTGGACT GGAGGTAGGA CCTGCTTCTC TTCTGTCGCA AGGTGGATGC
    TTGAACCAAG ATCGAAAGTT AACAAAGTCT GAGAGGCAGC GATTTAAAGA AGAGGCTGAA
    ATGTTAAAGG GTCTTCAGCA TCCCAATATT GTTCGATTCT ATGATTCCTG GGAATCCACA
    GTAAAGGGAA AGAAGTGCAT TGTGTTGGTG ACTGAACTGA TGACATCTGG AACTCTTAAA
    ACGTACCTGA AAAGGTTTAA GGTGATGAAG ATCAAAGTTT TGAGAAGCTG GTGCCGTCAG
    ATTCTTAAAG GACTTCAGTT TCTTCATACT AGAACTCCAC CTATTATTCA CCGGGATCTT
    AAGTGTGACA ACATCTTTAT CACTGGCCCC ACAGGCTCAG TCAAGATTGG AGACCTAGGT
    CTGGCCACCC TGAAGCGGGC TTCTTTTGCC AAGAGTGTGA TAGGTACCCC AGAGTTCATG
    GCCCCTGAGA TGTATGAAGA GAAGTACGAT GAATCCGTTG ATGTTTATGC TTTTGGGATG
    TGCATGCTCG AGATGGCCAC GTCTGAATAC CCGTACTCAG AGTGTCAGAA CGCTGCACAG
    ATCTACCGTC GTGTGACCAG CGGGGTGAAG CCAGCCAGTT TTGACAAAGT AGCAATTCCT
    GAAGTGAAGG AAATTATTGA AGGATGCATA CGGCAAAACA AAGATGAAAG ATACTCTATC
    AAAGACCTTT TGAACCATGC CTTCTTCCAG GAGGAAACAG GGGTACGGGT AGAATTAGCA
    GAAGAAGATG ATGGAGAAAA GATAGCCATT AAATTATGGC TACGTATTGA AGATATTAAG
    AAACTAAAGG GGAAATACAA AGACAATGAA GCTATCGAGT TTTCCTTTGA CTTGGAGAAA
    GATGTGCCAG AAGACGTTGC TCAAGAAATG GTAGATTCTG GGTATGTCTG TGAAGGTGAT
    CACAAGACCA TGGCAAAAGC CATCAAAGAT AGAGTGTCGT TAATTAAGAG AAAACGAGAA
    CAGCGTCAAT TAGTGCGGGA GGAGCAAGAA AAAAGAAAGC AGGAGGAGAG CAGCTTCAAG
    CAGCAGGTGG AGCAGCAGCC CAGCACTGCC CAGGCCGGAG TCCAGCAGCC CCCTGCCACC
    AGCACTGGTG GACCCGCTGC CTCTGCTGCG TCTGCGTCCG TCTCCACGCA GGTAGAGCCA
    GAAGAGCCTG AGGCCGACCA GCACCAGCAG CTGCAGTACC AGCAGCCTGG TGTGTCTGTG
    ATATCTGACG GGACGGTTGA CAGTGGTCAG GGGTCTTCTG TCTTCACGGA ATCTCGAGTC
    AGCAGCCAAC AGACAGTTTC GTACGGGTCC CAGCATGAGC AGGCACCTTC CACTGGCGCC
    CTCCCAGGGC ACACAGCATC TGTTGTCCAA ACGCAGGCTC AGCCCCATGC GGTATATCCA
    CCCTCAAGTT TGCCTCAGTC CATGGCGCAT CCGCGTGGGG GGACCCCAAC ATACCCAGAA
    TCACAGATAT TTTTCCCAAC TATTCATGAA CGTCCAGTTT CTTTTTCACC ACCTCCCACC
    TGTCCACCGA AGGTGGCCAT TGCCCAGCGG CGTAAGAGCA CCTCCTTCTT GGAAGCCCAA
    ACTCACCAGT TCCAACCCCT GCTGAGGACT GTCGGGCAGA GTCTTCTTCC ACCTGGTGGC
    AGCCCACCTA ACTGGACTCC AGAGACTGTA GTTATGTGGG GCACTGCAGC CAGTAGAGTT
    GCTGGAGAGC TGTGTGAGGT GCAGGCCCAT CCTGTGTTCG AGCCAGCTCC AGTTTACAGT
    GACTACAGAG CTGGACTGAT GCTTCCAGAA GAAGCTCACT ACTTCCTCCC TCCAGAAGCA
    GTGTATCTAG CCGGGGTACA TTACCAGGCC CAGGTGGCAG AACAGTTTGA GGGTTTTCCA
    TACAGCTCAC CAGTCCTGTC AAGTCCTATG AAGCAGACAC CTGAGCAGAA GCCAGTGCAC
    GGGGGCCCCA CCTCCAGTTC TGTCTTTGAA TTTCCATCGG GACAGGCTTT CCTGGTAGGA
    CACCTCCAGA ATGTAAGATT AGATTCGGGA CTGAGCCCAG GCTCTCCCCA GTCTAGCGTT
    TCTGCACCTA TCAGTACAGA TGCCACACGC TTGAAGTTTC ACCCTGTCTT TGTTCCTCAT
    TCTGCACCTG CTGTGTTAAC TCATAACAAC GAGAGCAGAA GCAGTTGTGT GTTTGAGTTT
    CACGTTCATA CTCCAAGCTC CTCTTCAGGA GAAGGAGGGG GAGGAGTTTT ACCTCAGCGC
    ATTTACCGAA ATCGGCAGGT TGCTGTGGAC TCGAACCAGG AAGAACCACC TCTTCAACCT
    GCAGGACTAC ACAGCCGCCT GCAGCCTGTG ACTGAAGAAC AGCAGATGCC CCAGGCCCCA
    GAGTTGACCA TCTCTGTGGT GGAGCCCACC GGACAGACCT GGCCCATAGG AAGCCCAGAG
    TACTCCAGTG ATTCCTCCCA GGTCACTTCT TCAGACCCCA GTGAATTTCA GTCACCTCCC
    CCTACAGGGG GAGCAGCTGC TCCTTTTGGC TCTGACGTCT CATTACCCTT TATCCGCGTG
    CCTCAGACAG TGTTACAAGA ATCCCCACTC TTCTTCTGTT TCCCCCAAGG AGCCACCTCT
    CCGCAGGTTT TATCCGCCTC ATTTTCTTCA GGAGGATCTG CACTCCATCC ACAGGCACAG
    GGGCAGAGCC AGGGTCAGCC ATCTTCAAGT AGTTTAACAG GGGTTCCATC CTCCCAACCC
    ATGCAGCATT CTCAGCAGCA GCAAGGAGTG CAGCCAACAG CCCCCTCTCA GCAGGCGGTG
    CAGTATCCGC TCCCTCAGAC ATCGGCCCCC AGCGAGGCCA CGATTGCACA GCCTGTGAGT
    CAGCCTCCAC CTCCACAGGC CTTGCCACAC GTGTCCGCGG GAAAACAGCT TCCAGTTTCC
    CAGCCAGTAC CAACTCTCCA AGGCGAACCT CAGATCCCAG TTGCAACACA GCCCTCAGTC
    GTTCCCGTCC ATGCTGGTGC TCACTTCCTT CCTGTGGGAC AGCCACTCCC ACCCTCTTTA
    CTGCCCCAGT ACCCCGTCTC TCAGCTGCCC CCCATCTCTC AGCTCCCCTC TGCTCCTCCT
    CACGTGTCTG TGGCTCAGCC AGGCTTCCCA CCCCTGCCGG TCACGATGGC AGCTGGCGTT
    AGTCAGCCTC TGCTCACCTT GGCTTCATCT GCTGCGGCGG CGGCCGTCCC CGGGGCACCC
    ACTGTGGTCC CTAGTCAGCT TCCTGCCCTC CTGCAGCCTG TGACCCAGCT GCCGAGTCAG
    GCTCACCCGC AGCTCCTGCA GCCAGCAGTT CAGTCCATGG GGATCCCCGC CAGCCTTGGA
    CAGACTGCCG AGGTGCCACT TCCCCCTGGA GATATTCTCT ACCAGGGCTT CCCACCTCGA
    CTGCCATCGC AGTACCCAGG AGACTCAAAC ATTGCCCCCT CCTCCAGCGC AGCTTCTGTG
    TGCCTGCCTT CTGCAGTCCT GTCCCCTCCC ATGCCGACAG AGGCGCTGGC TGCCCCGGGT
    TACTTTCCTG CAATGGTGCA GCCCTATGTG GAATCCAGTC TCTTGGTTCC TGTGGGCAGT
    GTAGGAGGAC AGGTCCAGGT GTCCCAGCCG GCAGTGAGCT TAGCACAAGC CCCCACCACA
    TCCTCCCAGC AAGCAGCTCT GGAGAGCACT CAGGTCGTCT CTCAGGTGGC TCCTCCAGAG
    CCAGCTCCCG GGGCTCAGCC ACAGCCTGCC CAGCCAGCCG CGGTGGTCTC TTCTATAGAC
    AGCGCACATT CAGATGTGGC TTCAGGCCTG AGTGATGGCA ACGAGAATGT CCCCTCATCC
    AGCGGGAGGC ACGAGGGCAG GACCACGAAG CGGCTGCATC GGAAGTCTAT AAGGAGCCGC
    TCTCGTCACG AGAAGCCTTC CCGCCCCAAA CTGAGAATTC TCAATGTTTC AAATAAGGGA
    GATCGGGTGG TAGAATGTCA GTTAGAGACT CACAATCGGA AGATGGTTAC ATTCAAATTT
    GATTTAGATG GTGACAACCC TGAGGAGATA GCCACAATTA TGGTGAACAA TGACTTTATT
    CTGGCACTGG AGAGAGATGC GTTTGTGGAG CAAGTGCGGG AGATCATTGA GAAAGCTGAC
    GAGATGCTCA GTGAGGATGT CAGCGTGGAG CCAGAGGGTG ACCAGAGCTT GGAGAGTCTC
    CATGGCAAGG ATGACTATGG CTTCTCAGGC ACCCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CATCTTCCTT GCCACAACAG ATAGCAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGC CCTTGCAACC AGCTCTGTAT GTGCCCTCCA
    GCAGAGCCGA AATCAGGGAC CAGCAGCACC AACGCCGTTG GGGGAGCCGT GAGCAGCCAG
    GCCGCCCCGG CTCCACCTCC TGCCGTGACG TCCAGCAGGA AGGGCATGTT CACGGACGAC
    CTGCACAAGC TGGTGGACAA CTGGGCCCGG GACGCCATGA GTCTCTCAGG CAGGCGGGGA
    AGCAAAGGAC ACGTGAGCTA TGAGGGCCCT GGCATGGCAA GGAAGTTCTC TGCGCCCGGT
    CAGCTGTGCG TCTCCATGAC CTCAAACCTG GGCGGCTCTG CCCCCGGCTC TGCAGCGTCA
    GCTACCTCTC TAGGGCACTT CCCCAAGTCC ATGTGCCCCC CTCAGCAGTA CGGTTTCCCT
    ACCCCCCCGT TTGGCACGCA GTGGAGCGGG ACGGGTGGCC CGGCACCCCA GCCGCTTAGC
    CAGTTCCAGC CTGTGGGAAC AGCCTCCTTA CAGAATTTCA ACATCAGCAA CTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319026.1
    CDS6..7907
    Translation

    Target ORF information:

    RefSeq Version XM_015463540.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTGCCCAT GCCTAATTCA AGTCTTGGGA ATGGGACTCT GCGACTGGTT TAGGCCAGTC 
    GTGATTCCTT TCCTTGGACT GGAGGTAGGA CCTGCTTCTC TTCTGTCGCA AGGTGGATGC
    TTGAACCAAG ATCGAAAGTT AACAAAGTCT GAGAGGCAGC GATTTAAAGA AGAGGCTGAA
    ATGTTAAAGG GTCTTCAGCA TCCCAATATT GTTCGATTCT ATGATTCCTG GGAATCCACA
    GTAAAGGGAA AGAAGTGCAT TGTGTTGGTG ACTGAACTGA TGACATCTGG AACTCTTAAA
    ACGTACCTGA AAAGGTTTAA GGTGATGAAG ATCAAAGTTT TGAGAAGCTG GTGCCGTCAG
    ATTCTTAAAG GACTTCAGTT TCTTCATACT AGAACTCCAC CTATTATTCA CCGGGATCTT
    AAGTGTGACA ACATCTTTAT CACTGGCCCC ACAGGCTCAG TCAAGATTGG AGACCTAGGT
    CTGGCCACCC TGAAGCGGGC TTCTTTTGCC AAGAGTGTGA TAGGTACCCC AGAGTTCATG
    GCCCCTGAGA TGTATGAAGA GAAGTACGAT GAATCCGTTG ATGTTTATGC TTTTGGGATG
    TGCATGCTCG AGATGGCCAC GTCTGAATAC CCGTACTCAG AGTGTCAGAA CGCTGCACAG
    ATCTACCGTC GTGTGACCAG CGGGGTGAAG CCAGCCAGTT TTGACAAAGT AGCAATTCCT
    GAAGTGAAGG AAATTATTGA AGGATGCATA CGGCAAAACA AAGATGAAAG ATACTCTATC
    AAAGACCTTT TGAACCATGC CTTCTTCCAG GAGGAAACAG GGGTACGGGT AGAATTAGCA
    GAAGAAGATG ATGGAGAAAA GATAGCCATT AAATTATGGC TACGTATTGA AGATATTAAG
    AAACTAAAGG GGAAATACAA AGACAATGAA GCTATCGAGT TTTCCTTTGA CTTGGAGAAA
    GATGTGCCAG AAGACGTTGC TCAAGAAATG GTAGATTCTG GGTATGTCTG TGAAGGTGAT
    CACAAGACCA TGGCAAAAGC CATCAAAGAT AGAGTGTCGT TAATTAAGAG AAAACGAGAA
    CAGCGTCAAT TAGTGCGGGA GGAGCAAGAA AAAAGAAAGC AGGAGGAGAG CAGCTTCAAG
    CAGCAGGTGG AGCAGCAGCC CAGCACTGCC CAGGCCGGAG TCCAGCAGCC CCCTGCCACC
    AGCACTGGTG GACCCGCTGC CTCTGCTGCG TCTGCGTCCG TCTCCACGCA GGTAGAGCCA
    GAAGAGCCTG AGGCCGACCA GCACCAGCAG CTGCAGTACC AGCAGCCTGG TGTGTCTGTG
    ATATCTGACG GGACGGTTGA CAGTGGTCAG GGGTCTTCTG TCTTCACGGA ATCTCGAGTC
    AGCAGCCAAC AGACAGTTTC GTACGGGTCC CAGCATGAGC AGGCACCTTC CACTGGCGCC
    CTCCCAGGGC ACACAGCATC TGTTGTCCAA ACGCAGGCTC AGCCCCATGC GGTATATCCA
    CCCTCAAGTT TGCCTCAGTC CATGGCGCAT CCGCGTGGGG GGACCCCAAC ATACCCAGAA
    TCACAGATAT TTTTCCCAAC TATTCATGAA CGTCCAGTTT CTTTTTCACC ACCTCCCACC
    TGTCCACCGA AGGTGGCCAT TGCCCAGCGG CGTAAGAGCA CCTCCTTCTT GGAAGCCCAA
    ACTCACCAGT TCCAACCCCT GCTGAGGACT GTCGGGCAGA GTCTTCTTCC ACCTGGTGGC
    AGCCCACCTA ACTGGACTCC AGAGACTGTA GTTATGTGGG GCACTGCAGC CAGTAGAGTT
    GCTGGAGAGC TGTGTGAGGT GCAGGCCCAT CCTGTGTTCG AGCCAGCTCC AGTTTACAGT
    GACTACAGAG CTGGACTGAT GCTTCCAGAA GAAGCTCACT ACTTCCTCCC TCCAGAAGCA
    GTGTATCTAG CCGGGGTACA TTACCAGGCC CAGGTGGCAG AACAGTTTGA GGGTTTTCCA
    TACAGCTCAC CAGTCCTGTC AAGTCCTATG AAGCAGACAC CTGAGCAGAA GCCAGTGCAC
    GGGGGCCCCA CCTCCAGTTC TGTCTTTGAA TTTCCATCGG GACAGGCTTT CCTGGTAGGA
    CACCTCCAGA ATGTAAGATT AGATTCGGGA CTGAGCCCAG GCTCTCCCCA GTCTAGCGTT
    TCTGCACCTA TCAGTACAGA TGCCACACGC TTGAAGTTTC ACCCTGTCTT TGTTCCTCAT
    TCTGCACCTG CTGTGTTAAC TCATAACAAC GAGAGCAGAA GCAGTTGTGT GTTTGAGTTT
    CACGTTCATA CTCCAAGCTC CTCTTCAGGA GAAGGAGGGG GAGGAGTTTT ACCTCAGCGC
    ATTTACCGAA ATCGGCAGGT TGCTGTGGAC TCGAACCAGG AAGAACCACC TCTTCAACCT
    GCAGGACTAC ACAGCCGCCT GCAGCCTGTG ACTGAAGAAC AGCAGATGCC CCAGGCCCCA
    GAGTTGACCA TCTCTGTGGT GGAGCCCACC GGACAGACCT GGCCCATAGG AAGCCCAGAG
    TACTCCAGTG ATTCCTCCCA GGTCACTTCT TCAGACCCCA GTGAATTTCA GTCACCTCCC
    CCTACAGGGG GAGCAGCTGC TCCTTTTGGC TCTGACGTCT CATTACCCTT TATCCGCGTG
    CCTCAGACAG TGTTACAAGA ATCCCCACTC TTCTTCTGTT TCCCCCAAGG AGCCACCTCT
    CCGCAGGTTT TATCCGCCTC ATTTTCTTCA GGAGGATCTG CACTCCATCC ACAGGCACAG
    GGGCAGAGCC AGGGTCAGCC ATCTTCAAGT AGTTTAACAG GGGTTCCATC CTCCCAACCC
    ATGCAGCATT CTCAGCAGCA GCAAGGAGTG CAGCCAACAG CCCCCTCTCA GCAGGCGGTG
    CAGTATCCGC TCCCTCAGAC ATCGGCCCCC AGCGAGGCCA CGATTGCACA GCCTGTGAGT
    CAGCCTCCAC CTCCACAGGC CTTGCCACAC GTGTCCGCGG GAAAACAGCT TCCAGTTTCC
    CAGCCAGTAC CAACTCTCCA AGGCGAACCT CAGATCCCAG TTGCAACACA GCCCTCAGTC
    GTTCCCGTCC ATGCTGGTGC TCACTTCCTT CCTGTGGGAC AGCCACTCCC ACCCTCTTTA
    CTGCCCCAGT ACCCCGTCTC TCAGCTGCCC CCCATCTCTC AGCTCCCCTC TGCTCCTCCT
    CACGTGTCTG TGGCTCAGCC AGGCTTCCCA CCCCTGCCGG TCACGATGGC AGCTGGCGTT
    AGTCAGCCTC TGCTCACCTT GGCTTCATCT GCTGCGGCGG CGGCCGTCCC CGGGGCACCC
    ACTGTGGTCC CTAGTCAGCT TCCTGCCCTC CTGCAGCCTG TGACCCAGCT GCCGAGTCAG
    GCTCACCCGC AGCTCCTGCA GCCAGCAGTT CAGTCCATGG GGATCCCCGC CAGCCTTGGA
    CAGACTGCCG AGGTGCCACT TCCCCCTGGA GATATTCTCT ACCAGGGCTT CCCACCTCGA
    CTGCCATCGC AGTACCCAGG AGACTCAAAC ATTGCCCCCT CCTCCAGCGC AGCTTCTGTG
    TGCCTGCCTT CTGCAGTCCT GTCCCCTCCC ATGCCGACAG AGGCGCTGGC TGCCCCGGGT
    TACTTTCCTG CAATGGTGCA GCCCTATGTG GAATCCAGTC TCTTGGTTCC TGTGGGCAGT
    GTAGGAGGAC AGGTCCAGGT GTCCCAGCCG GCAGTGAGCT TAGCACAAGC CCCCACCACA
    TCCTCCCAGC AAGCAGCTCT GGAGAGCACT CAGGTCGTCT CTCAGGTGGC TCCTCCAGAG
    CCAGCTCCCG GGGCTCAGCC ACAGCCTGCC CAGCCAGCCG CGGTGGTCTC TTCTATAGAC
    AGCGCACATT CAGATGTGGC TTCAGGCCTG AGTGATGGCA ACGAGAATGT CCCCTCATCC
    AGCGGGAGGC ACGAGGGCAG GACCACGAAG CGGCTGCATC GGAAGTCTAT AAGGAGCCGC
    TCTCGTCACG AGAAGCCTTC CCGCCCCAAA CTGAGAATTC TCAATGTTTC AAATAAGGGA
    GATCGGGTGG TAGAATGTCA GTTAGAGACT CACAATCGGA AGATGGTTAC ATTCAAATTT
    GATTTAGATG GTGACAACCC TGAGGAGATA GCCACAATTA TGGTGAACAA TGACTTTATT
    CTGGCACTGG AGAGAGATGC GTTTGTGGAG CAAGTGCGGG AGATCATTGA GAAAGCTGAC
    GAGATGCTCA GTGAGGATGT CAGCGTGGAG CCAGAGGGTG ACCAGAGCTT GGAGAGTCTC
    CATGGCAAGG ATGACTATGG CTTCTCAGGC ACCCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CATCTTCCTT GCCACAACAG ATAGCAGGCA TTCCTGCCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCTGG AAGACGGTTT ATAGTAAGTC CTGTGCCAGA AAGTCGATTA
    CGAGAATCAA AACTTTTCAC TGAAATTTCA GATACGGTTG CTGCTTCCAC ATCTGCTGGC
    CCTGGAATGA ACTTGTCTCA CTCTGCCTCA TCCCTTAGTC TGCAGCAGGC CTTTTCTGAA
    CTTAGACATG CTCGGATGGC AGAAGGGCCG AGTACGGCCC CTCCCCACTT TGGTCAGACA
    GGCCCCGTGT TTCCAGCAGT GCCTCCCACC CTGAGCAGTG CCCCTGGAGC CCCAGCCGCA
    GCAGCCGCAG CCTCGGTGTC AGCCCCTGCC CCAAGCCACC CTCTTAGTGA CGTGACCACA
    TCGGTCATTC AGACTGAGGT GACAGTGGCC CCTGGAACAG GTGTCATGCC TCCCAGTGGT
    CTCCCTGTAC CCCCTGTGTC TGAGTCCCTG GCACCTTCCA GCGTGGCCCC CAGCGTCACC
    ATCCCTGCAG TTCTCTCGGC ACTACCAGCG CCCCAGGCGG CACAGGCTCC CACCCCTGGC
    AGCGGGGCCT CCAGTGCAGG CACTTTCCCC TCTGCACCCG CATCCCTGAC ACCAGCCTCT
    GCTGTGGGCA GCGCTGTTGC CTCTGCTGCT CAGCTTCCGC CTGTGCCGTC TCAGCCCGCG
    GTAGGCAGCT CGACCGGGGT GGCCACGCTG ACAGCCGTGC CCGCTGCCGC TCTGGGCCCT
    AGCCTCACGG CACTGCCGTC CCTTGCTCTC AGCAGCAGTA CCTCTGCCCC AGCTCTGGCA
    GAGACAGTGC TGGTCAGTGC CCACGCACTG GATAAGACGG CCCATAGTAG CACCACTGGC
    CTGGCTCTGT CCCTTCCTGC CACCTCGTCC TCTCCCGCCC CTGGAGCAGG AGTGTCCAGT
    GCTGTTTCTC AGCCTACTGC TGCGGCTCAC CCTTTGGTCC TCCCATCAGT CGTAACCTCT
    ACTCCCGTAC TCTCCCAGGC AGGACCCATG TCAGCACCTC TACTGCCCCA GGTACCCGGT
    ATTCCACCCC TGGTACAACC TGTTGTCAGC GTGCCTGCTG TGCAACAGAC CCTGGTTCAT
    AGTCAGCCTC AGCCCGCTCC ACTCCCCAAC CAGCCCCACA CTCACTGTCC CGAGATAGAC
    GCCGACACTC AACCCAAAGC TCCCGGGATC GACGACATCA AGACTTTGGA GGAAAAGCTG
    CGATCTCTCT TTAGTGAGCA CAGTTCTTCT GGAGCCCAAC ACGCCCCCGT CTCTCTGGAG
    ACCTCGCTCA CTGTAGAGAC CGCTGTCACA GCAGGCCTCC CAACCACTGC TGTTGCACCA
    AGCAAGCCTC TGACTTCCAC TGCCAGTACT TGCTTGCCAC CAACCAGTCT GCCCTTAGGA
    GCAGCTGGCT TGTCAGTTAT ACCAATGGTC ACTCCTGGTC AAGTTTCCAC CCCAGTCAGC
    TCTGCGCCAG GAGTGAAACT TGAAACTGCT CCAGCAAAGC CACCTTTAAC TAAGCCTCCA
    GTGCTGCCCA TGGGCACTGA ACTTCCAGCT GGTACTCCAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCACTAAC TCAGCCCCCG CAGCCCCTGG GAGACCTGGA CGCTCAGCTG
    AGAAGAACCC TCAGTCCCGA GAGTGGCATG CTGACGTCTG CCGTTGGACC CGTGTCTGTG
    GTGGCTCCAA CAGCAGTTGT GGAGACCGAA GCCCAACTTC CAAAGGATGT TCTTTCAGTC
    TCTCAAGTCA CAGAAGCTCC TGTCCTTGCG CCCACTTCCA CAGCTGGTGT TCTGAAGATG
    GGGCGATTTC AGGTCTCAGT TGCAATGGAT GACCCCCAGA AGGAGGGTAA AAGTAAGTCG
    GAGGATGCCA AGTCTGTCCA CTTCGCATCC AGCACGTCAG AGTCCTCGGT GCTGTCAAGC
    AGCAGCCCCG AGAGCACCGT GGTGAAAGCA GAGCCCAACG GGCTGGCCGT CCGGGGCGCC
    TCTGCAGACA TGCCTGACAG CGCCCACCAG CCACCTGCCT CAGAAGCTGG GCAGCCTAGC
    AAGGTGGGCC GTTTTCAGGT GACCACCACA GCGAGCAGAG TGGGTCGCTT CTCTGTGTCC
    AGAACTGAAG ACAAGATGGC TGAGGTGAAG GGAGAGGAGC CAGTGGTCTC TCCTTTCATG
    GACTCAGAAC ACGACATTCC TCCCACCGTG ACGCCAAAGA AAGAGAAACC CGAACTGTCT
    GAGCCTTCGC ATCTGAACGG GCCGTCTTCC GACCTGGAGG CTGCCTTCCT CAGCCGGGAC
    GTGGATGCTG GCTCAGGGAG CCCTCACTCC CCTCCGCAGC TGAGCTCCAA GAGCCTTCCT
    GCCCAGAGCC TCAGTCAGAG CCTCAGCAAT TCCTTCAACT CTTCCTACAT GAGCAGCGAC
    AATGAGTCAG ACATTGAAGA CGAGGACTTA AAGTTAGAGC TTCGCCGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ACGAAATTGA GTCTTTATAC
    ACCAAACTGG GCAAGGTTCC TCCTGCTGTC ATCATTCCCC CAGCAGCCCC CCTTGCAGGG
    AGAAGGAGGC GACCCACCAA AAGCAAAGGG AGCAAGTCCA GTCGCAGCAG CTCTTTGGGG
    AATAAAAGCC CTGGACCAGG TACCCTGTCT GGCCAGAGTG CAGCTTCAGT CTTGCACCCT
    CAGCAGACCC TCCCCGCTCC TGGCAGCGTC CCGGAGACAG GGCCCAACCA GCTGTTACAG
    CCTGTGAAGC CATCTCCTTC CAGTGACAAC CTGTACTCGG CCTTTACCAG CGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCGCCAGGT CAAGGCTGTG CCAAGTTTAA CTGTGCGTCC
    GAGCAGGTGA CGTTCAAAGC TGGCGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGC CCTTGCAACC AGCTCTGTAT GTGCCCTCCA
    GCAGAGCCGA AATCAGGGAC CAGCAGCACC AACGCCGTTG GGGGAGCCGT GAGCAGCCAG
    GCCGCCCCGG CTCCACCTCC TGCCGTGACG TCCAGCAGGA AGGGCATGTT CACGGACGAC
    CTGCACAAGC TGGTGGACAA CTGGGCCCGG GACGCCATGA GTCTCTCAGG CAGGCGGGGA
    AGCAAAGGAC ACGTGAGCTA TGAGGGCCCT GGCATGGCAA GGAAGTTCTC TGCGCCCGGT
    CAGCTGTGCG TCTCCATGAC CTCAAACCTG GGCGGCTCTG CCCCCGGCTC TGCAGCGTCA
    GCTACCTCTC TAGGGCACTT CCCCAAGTCC ATGTGCCCCC CTCAGCAGTA CGGTTTCCCT
    ACCCCCCCGT TTGGCACGCA GTGGAGCGGG ACGGGTGGCC CGGCACCCCA GCCGCTTAGC
    CAGTTCCAGC CTGTGGGAAC AGCCTCCTTA CAGAATTTCA ACATCAGCAA CTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89988
    Clone ID Related Accession (Same CDS sequence) XM_015463543.1 , XM_015471359.1
    Accession Version XM_015463543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGATTTTA CAAAGAGAGA TTTTTGCTCA GTGTTTGTCT TCGTAAATTC TCATTGCTGC 
    TGCTGCTCTC AAAAGGATTG TATAAATGAG GGGGTGAAGC CAGCCAGTTT TGACAAAGTA
    GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAC GGCAAAACAA AGATGAAAGA
    TACTCTATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTACGGGTA
    GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AATTATGGCT ACGTATTGAA
    GATATTAAGA AACTAAAGGG GAAATACAAA GACAATGAAG CTATCGAGTT TTCCTTTGAC
    TTGGAGAAAG ATGTGCCAGA AGACGTTGCT CAAGAAATGG TAGATTCTGG GTATGTCTGT
    GAAGGTGATC ACAAGACCAT GGCAAAAGCC ATCAAAGATA GAGTGTCGTT AATTAAGAGA
    AAACGAGAAC AGCGTCAATT AGTGCGGGAG GAGCAAGAAA AAAGAAAGCA GGAGGAGAGC
    AGCTTCAAGC AGCAGGTGGA GCAGCAGCCC AGCACTGCCC AGGCCGGAGT CCAGCAGCCC
    CCTGCCACCA GCACTGGTGG ACCCGCTGCC TCTGCTGCGT CTGCGTCCGT CTCCACGCAG
    GTAGAGCCAG AAGAGCCTGA GGCCGACCAG CACCAGCAGC TGCAGTACCA GCAGCCTGGT
    GTGTCTGTGA TATCTGACGG GACGGTTGAC AGTGGTCAGG GGTCTTCTGT CTTCACGGAA
    TCTCGAGTCA GCAGCCAACA GACAGTTTCG TACGGGTCCC AGCATGAGCA GGCACCTTCC
    ACTGGCGCCC TCCCAGGGCA CACAGCATCT GTTGTCCAAA CGCAGGCTCA GCCCCATGCG
    GTATATCCAC CCTCAAGTTT GCCTCAGTCC ATGGCGCATC CGCGTGGGGG GACCCCAACA
    TACCCAGAAT CACAGATATT TTTCCCAACT ATTCATGAAC GTCCAGTTTC TTTTTCACCA
    CCTCCCACCT GTCCACCGAA GGTGGCCATT GCCCAGCGGC GTAAGAGCAC CTCCTTCTTG
    GAAGCCCAAA CTCACCAGTT CCAACCCCTG CTGAGGACTG TCGGGCAGAG TCTTCTTCCA
    CCTGGTGGCA GCCCACCTAA CTGGACTCCA GAGACTGTAG TTATGTGGGG CACTGCAGCC
    AGTAGAGTTG CTGGAGAGCT GTGTGAGGTG CAGGCCCATC CTGTGTTCGA GCCAGCTCCA
    GTTTACAGTG ACTACAGAGC TGGACTGATG CTTCCAGAAG AAGCTCACTA CTTCCTCCCT
    CCAGAAGCAG TGTATCTAGC CGGGGTACAT TACCAGGCCC AGGTGGCAGA ACAGTTTGAG
    GGTTTTCCAT ACAGCTCACC AGTCCTGTCA AGTCCTATGA AGCAGACACC TGAGCAGAAG
    CCAGTGCACG GGGGCCCCAC CTCCAGTTCT GTCTTTGAAT TTCCATCGGG ACAGGCTTTC
    CTGGTAGGAC ACCTCCAGAA TGTAAGATTA GATTCGGGAC TGAGCCCAGG CTCTCCCCAG
    TCTAGCGTTT CTGCACCTAT CAGTACAGAT GCCACACGCT TGAAGTTTCA CCCTGTCTTT
    GTTCCTCATT CTGCACCTGC TGTGTTAACT CATAACAACG AGAGCAGAAG CAGTTGTGTG
    TTTGAGTTTC ACGTTCATAC TCCAAGCTCC TCTTCAGGAG AAGGAGGGGG AGGAGTTTTA
    CCTCAGCGCA TTTACCGAAA TCGGCAGGTT GCTGTGGACT CGAACCAGGA AGAACCACCT
    CTTCAACCTG CAGGACTACA CAGCCGCCTG CAGCCTGTGA CTGAAGAACA GCAGATGCCC
    CAGGCCCCAG AGTTGACCAT CTCTGTGGTG GAGCCCACCG GACAGACCTG GCCCATAGGA
    AGCCCAGAGT ACTCCAGTGA TTCCTCCCAG GTCACTTCTT CAGACCCCAG TGAATTTCAG
    TCACCTCCCC CTACAGGGGG AGCAGCTGCT CCTTTTGGCT CTGACGTCTC ATTACCCTTT
    ATCCGCGTGC CTCAGACAGT GTTACAAGAA TCCCCACTCT TCTTCTGTTT CCCCCAAGGA
    GCCACCTCTC CGCAGGTTTT ATCCGCCTCA TTTTCTTCAG GAGGATCTGC ACTCCATCCA
    CAGGCACAGG GGCAGAGCCA GGGTCAGCCA TCTTCAAGTA GTTTAACAGG GGTTCCATCC
    TCCCAACCCA TGCAGCATTC TCAGCAGCAG CAAGGAGTGC AGCCAACAGC CCCCTCTCAG
    CAGGCGGTGC AGTATCCGCT CCCTCAGACA TCGGCCCCCA GCGAGGCCAC GATTGCACAG
    CCTGTGAGTC AGCCTCCACC TCCACAGGCC TTGCCACACG TGTCCGCGGG AAAACAGCTT
    CCAGTTTCCC AGCCAGTACC AACTCTCCAA GGCGAACCTC AGATCCCAGT TGCAACACAG
    CCCTCAGTCG TTCCCGTCCA TGCTGGTGCT CACTTCCTTC CTGTGGGACA GCCACTCCCA
    CCCTCTTTAC TGCCCCAGTA CCCCGTCTCT CAGCTGCCCC CCATCTCTCA GCTCCCCTCT
    GCTCCTCCTC ACGTGTCTGT GGCTCAGCCA GGCTTCCCAC CCCTGCCGGT CACGATGGCA
    GCTGGCGTTA GTCAGCCTCT GCTCACCTTG GCTTCATCTG CTGCGGCGGC GGCCGTCCCC
    GGGGCACCCA CTGTGGTCCC TAGTCAGCTT CCTGCCCTCC TGCAGCCTGT GACCCAGCTG
    CCGAGTCAGG CTCACCCGCA GCTCCTGCAG CCAGCAGTTC AGTCCATGGG GATCCCCGCC
    AGCCTTGGAC AGACTGCCGA GGTGCCACTT CCCCCTGGAG ATATTCTCTA CCAGGGCTTC
    CCACCTCGAC TGCCATCGCA GTACCCAGGA GACTCAAACA TTGCCCCCTC CTCCAGCGCA
    GCTTCTGTGT GCCTGCCTTC TGCAGTCCTG TCCCCTCCCA TGCCGACAGA GGCGCTGGCT
    GCCCCGGGTT ACTTTCCTGC AATGGTGCAG CCCTATGTGG AATCCAGTCT CTTGGTTCCT
    GTGGGCAGTG TAGGAGGACA GGTCCAGGTG TCCCAGCCGG CAGTGAGCTT AGCACAAGCC
    CCCACCACAT CCTCCCAGCA AGCAGCTCTG GAGAGCACTC AGGTCGTCTC TCAGGTGGCT
    CCTCCAGAGC CAGCTCCCGG GGCTCAGCCA CAGCCTGCCC AGCCAGCCGC GGTGGTCTCT
    TCTATAGACA GCGCACATTC AGATGTGGCT TCAGGCCTGA GTGATGGCAA CGAGAATGTC
    CCCTCATCCA GCGGGAGGCA CGAGGGCAGG ACCACGAAGC GGCTGCATCG GAAGTCTATA
    AGGAGCCGCT CTCGTCACGA GAAGCCTTCC CGCCCCAAAC TGAGAATTCT CAATGTTTCA
    AATAAGGGAG ATCGGGTGGT AGAATGTCAG TTAGAGACTC ACAATCGGAA GATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAACCCT GAGGAGATAG CCACAATTAT GGTGAACAAT
    GACTTTATTC TGGCACTGGA GAGAGATGCG TTTGTGGAGC AAGTGCGGGA GATCATTGAG
    AAAGCTGACG AGATGCTCAG TGAGGATGTC AGCGTGGAGC CAGAGGGTGA CCAGAGCTTG
    GAGAGTCTCC ATGGCAAGGA TGACTATGGC TTCTCAGGCA CCCAGAAATT GGAAGGAGAG
    TTCAAACAAC CAATTCCTGC ATCTTCCTTG CCACAACAGA TAGCAGGCAT TCCTGCCAGT
    TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTAAGTCC TGTGCCAGAA
    AGTCGATTAC GAGAATCAAA ACTTTTCACT GAAATTTCAG ATACGGTTGC TGCTTCCACA
    TCTGCTGGCC CTGGAATGAA CTTGTCTCAC TCTGCCTCAT CCCTTAGTCT GCAGCAGGCC
    TTTTCTGAAC TTAGACATGC TCGGATGGCA GAAGGGCCGA GTACGGCCCC TCCCCACTTT
    GGTCAGACAG GCCCCGTGTT TCCAGCAGTG CCTCCCACCC TGAGCAGTGC CCCTGGAGCC
    CCAGCCGCAG CAGCCGCAGC CTCGGTGTCA GCCCCTGCCC CAAGCCACCC TCTTAGTGAC
    GTGACCACAT CGGTCATTCA GACTGAGGTG ACAGTGGCCC CTGGAACAGG TGTCATGCCT
    CCCAGTGGTC TCCCTGTACC CCCTGTGTCT GAGTCCCTGG CACCTTCCAG CGTGGCCCCC
    AGCGTCACCA TCCCTGCAGT TCTCTCGGCA CTACCAGCGC CCCAGGCGGC ACAGGCTCCC
    ACCCCTGGCA GCGGGGCCTC CAGTGCAGGC ACTTTCCCCT CTGCACCCGC ATCCCTGACA
    CCAGCCTCTG CTGTGGGCAG CGCTGTTGCC TCTGCTGCTC AGCTTCCGCC TGTGCCGTCT
    CAGCCCGCGG TAGGCAGCTC GACCGGGGTG GCCACGCTGA CAGCCGTGCC CGCTGCCGCT
    CTGGGCCCTA GCCTCACGGC ACTGCCGTCC CTTGCTCTCA GCAGCAGTAC CTCTGCCCCA
    GCTCTGGCAG AGACAGTGCT GGTCAGTGCC CACGCACTGG ATAAGACGGC CCATAGTAGC
    ACCACTGGCC TGGCTCTGTC CCTTCCTGCC ACCTCGTCCT CTCCCGCCCC TGGAGCAGGA
    GTGTCCAGTG CTGTTTCTCA GCCTACTGCT GCGGCTCACC CTTTGGTCCT CCCATCAGTC
    GTAACCTCTA CTCCCGTACT CTCCCAGGCA GGACCCATGT CAGCACCTCT ACTGCCCCAG
    GTACCCGGTA TTCCACCCCT GGTACAACCT GTTGTCAGCG TGCCTGCTGT GCAACAGACC
    CTGGTTCATA GTCAGCCTCA GCCCGCTCCA CTCCCCAACC AGCCCCACAC TCACTGTCCC
    GAGATAGACG CCGACACTCA ACCCAAAGCT CCCGGGATCG ACGACATCAA GACTTTGGAG
    GAAAAGCTGC GATCTCTCTT TAGTGAGCAC AGTTCTTCTG GAGCCCAACA CGCCCCCGTC
    TCTCTGGAGA CCTCGCTCAC TGTAGAGACC GCTGTCACAG CAGGCCTCCC AACCACTGCT
    GTTGCACCAA GCAAGCCTCT GACTTCCACT GCCAGTACTT GCTTGCCACC AACCAGTCTG
    CCCTTAGGAG CAGCTGGCTT GTCAGTTATA CCAATGGTCA CTCCTGGTCA AGTTTCCACC
    CCAGTCAGCT CTGCGCCAGG AGTGAAACTT GAAACTGCTC CAGCAAAGCC ACCTTTAACT
    AAGCCTCCAG TGCTGCCCAT GGGCACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGCCCCCGC AGCCCCTGGG AGACCTGGAC
    GCTCAGCTGA GAAGAACCCT CAGTCCCGAG AGTGGCATGC TGACGTCTGC CGTTGGACCC
    GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG GAGACCGAAG CCCAACTTCC AAAGGATGTT
    CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT GTCCTTGCGC CCACTTCCAC AGCTGGTGTT
    CTGAAGATGG GGCGATTTCA GGTCTCAGTT GCAATGGATG ACCCCCAGAA GGAGGGTAAA
    AGTAAGTCGG AGGATGCCAA GTCTGTCCAC TTCGCATCCA GCACGTCAGA GTCCTCGGTG
    CTGTCAAGCA GCAGCCCCGA GAGCACCGTG GTGAAAGCAG AGCCCAACGG GCTGGCCGTC
    CGGGGCGCCT CTGCAGACAT GCCTGACAGC GCCCACCAGC CACCTGCCTC AGAAGCTGGG
    CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG ACCACCACAG CGAGCAGAGT GGGTCGCTTC
    TCTGTGTCCA GAACTGAAGA CAAGATGGCT GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT
    CCTTTCATGG ACTCAGAACA CGACATTCCT CCCACCGTGA CGCCAAAGAA AGAGAAACCC
    GAACTGTCTG AGCCTTCGCA TCTGAACGGG CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC
    AGCCGGGACG TGGATGCTGG CTCAGGGAGC CCTCACTCCC CTCCGCAGCT GAGCTCCAAG
    AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC CTCAGCAATT CCTTCAACTC TTCCTACATG
    AGCAGCGACA ATGAGTCAGA CATTGAAGAC GAGGACTTAA AGTTAGAGCT TCGCCGACTA
    CGGGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA CGAAATTGAG
    TCTTTATACA CCAAACTGGG CAAGGTTCCT CCTGCTGTCA TCATTCCCCC AGCAGCCCCC
    CTTGCAGGGA GAAGGAGGCG ACCCACCAAA AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC
    TCTTTGGGGA ATAAAAGCCC TGGACCAGGT ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC
    TTGCACCCTC AGCAGACCCT CCCCGCTCCT GGCAGCGTCC CGGAGACAGG GCCCAACCAG
    CTGTTACAGC CTGTGAAGCC ATCTCCTTCC AGTGACAACC TGTACTCGGC CTTTACCAGC
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCGCCAGGTC AAGGCTGTGC CAAGTTTAAC
    TGTGCGTCCG AGCAGGTGAC GTTCAAAGCT GGCGGCAGGA GGACGCGATT TCTGAGTACG
    CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CTTGCAACCA GCTCTGTATG
    TGCCCTCCAG CAGAGCCGAA ATCAGGGACC AGCAGCACCA ACGCCGTTGG GGGAGCCGTG
    AGCAGCCAGG CCGCCCCGGC TCCACCTCCT GCCGTGACGT CCAGCAGGAA GGGCATGTTC
    ACGGACGACC TGCACAAGCT GGTGGACAAC TGGGCCCGGG ACGCCATGAG TCTCTCAGGC
    AGGCGGGGAA GCAAAGGACA CGTGAGCTAT GAGGGCCCTG GCATGGCAAG GAAGTTCTCT
    GCGCCCGGTC AGCTGTGCGT CTCCATGACC TCAAACCTGG GCGGCTCTGC CCCCGGCTCT
    GCAGCGTCAG CTACCTCTCT AGGGCACTTC CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC
    GGTTTCCCTA CCCCCCCGTT TGGCACGCAG TGGAGCGGGA CGGGTGGCCC GGCACCCCAG
    CCGCTTAGCC AGTTCCAGCC TGTGGGAACA GCCTCCTTAC AGAATTTCAA CATCAGCAAC
    TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319029.1
    CDS311..7621
    Translation

    Target ORF information:

    RefSeq Version XM_015463543.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGATTTTA CAAAGAGAGA TTTTTGCTCA GTGTTTGTCT TCGTAAATTC TCATTGCTGC 
    TGCTGCTCTC AAAAGGATTG TATAAATGAG GGGGTGAAGC CAGCCAGTTT TGACAAAGTA
    GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAC GGCAAAACAA AGATGAAAGA
    TACTCTATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTACGGGTA
    GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AATTATGGCT ACGTATTGAA
    GATATTAAGA AACTAAAGGG GAAATACAAA GACAATGAAG CTATCGAGTT TTCCTTTGAC
    TTGGAGAAAG ATGTGCCAGA AGACGTTGCT CAAGAAATGG TAGATTCTGG GTATGTCTGT
    GAAGGTGATC ACAAGACCAT GGCAAAAGCC ATCAAAGATA GAGTGTCGTT AATTAAGAGA
    AAACGAGAAC AGCGTCAATT AGTGCGGGAG GAGCAAGAAA AAAGAAAGCA GGAGGAGAGC
    AGCTTCAAGC AGCAGGTGGA GCAGCAGCCC AGCACTGCCC AGGCCGGAGT CCAGCAGCCC
    CCTGCCACCA GCACTGGTGG ACCCGCTGCC TCTGCTGCGT CTGCGTCCGT CTCCACGCAG
    GTAGAGCCAG AAGAGCCTGA GGCCGACCAG CACCAGCAGC TGCAGTACCA GCAGCCTGGT
    GTGTCTGTGA TATCTGACGG GACGGTTGAC AGTGGTCAGG GGTCTTCTGT CTTCACGGAA
    TCTCGAGTCA GCAGCCAACA GACAGTTTCG TACGGGTCCC AGCATGAGCA GGCACCTTCC
    ACTGGCGCCC TCCCAGGGCA CACAGCATCT GTTGTCCAAA CGCAGGCTCA GCCCCATGCG
    GTATATCCAC CCTCAAGTTT GCCTCAGTCC ATGGCGCATC CGCGTGGGGG GACCCCAACA
    TACCCAGAAT CACAGATATT TTTCCCAACT ATTCATGAAC GTCCAGTTTC TTTTTCACCA
    CCTCCCACCT GTCCACCGAA GGTGGCCATT GCCCAGCGGC GTAAGAGCAC CTCCTTCTTG
    GAAGCCCAAA CTCACCAGTT CCAACCCCTG CTGAGGACTG TCGGGCAGAG TCTTCTTCCA
    CCTGGTGGCA GCCCACCTAA CTGGACTCCA GAGACTGTAG TTATGTGGGG CACTGCAGCC
    AGTAGAGTTG CTGGAGAGCT GTGTGAGGTG CAGGCCCATC CTGTGTTCGA GCCAGCTCCA
    GTTTACAGTG ACTACAGAGC TGGACTGATG CTTCCAGAAG AAGCTCACTA CTTCCTCCCT
    CCAGAAGCAG TGTATCTAGC CGGGGTACAT TACCAGGCCC AGGTGGCAGA ACAGTTTGAG
    GGTTTTCCAT ACAGCTCACC AGTCCTGTCA AGTCCTATGA AGCAGACACC TGAGCAGAAG
    CCAGTGCACG GGGGCCCCAC CTCCAGTTCT GTCTTTGAAT TTCCATCGGG ACAGGCTTTC
    CTGGTAGGAC ACCTCCAGAA TGTAAGATTA GATTCGGGAC TGAGCCCAGG CTCTCCCCAG
    TCTAGCGTTT CTGCACCTAT CAGTACAGAT GCCACACGCT TGAAGTTTCA CCCTGTCTTT
    GTTCCTCATT CTGCACCTGC TGTGTTAACT CATAACAACG AGAGCAGAAG CAGTTGTGTG
    TTTGAGTTTC ACGTTCATAC TCCAAGCTCC TCTTCAGGAG AAGGAGGGGG AGGAGTTTTA
    CCTCAGCGCA TTTACCGAAA TCGGCAGGTT GCTGTGGACT CGAACCAGGA AGAACCACCT
    CTTCAACCTG CAGGACTACA CAGCCGCCTG CAGCCTGTGA CTGAAGAACA GCAGATGCCC
    CAGGCCCCAG AGTTGACCAT CTCTGTGGTG GAGCCCACCG GACAGACCTG GCCCATAGGA
    AGCCCAGAGT ACTCCAGTGA TTCCTCCCAG GTCACTTCTT CAGACCCCAG TGAATTTCAG
    TCACCTCCCC CTACAGGGGG AGCAGCTGCT CCTTTTGGCT CTGACGTCTC ATTACCCTTT
    ATCCGCGTGC CTCAGACAGT GTTACAAGAA TCCCCACTCT TCTTCTGTTT CCCCCAAGGA
    GCCACCTCTC CGCAGGTTTT ATCCGCCTCA TTTTCTTCAG GAGGATCTGC ACTCCATCCA
    CAGGCACAGG GGCAGAGCCA GGGTCAGCCA TCTTCAAGTA GTTTAACAGG GGTTCCATCC
    TCCCAACCCA TGCAGCATTC TCAGCAGCAG CAAGGAGTGC AGCCAACAGC CCCCTCTCAG
    CAGGCGGTGC AGTATCCGCT CCCTCAGACA TCGGCCCCCA GCGAGGCCAC GATTGCACAG
    CCTGTGAGTC AGCCTCCACC TCCACAGGCC TTGCCACACG TGTCCGCGGG AAAACAGCTT
    CCAGTTTCCC AGCCAGTACC AACTCTCCAA GGCGAACCTC AGATCCCAGT TGCAACACAG
    CCCTCAGTCG TTCCCGTCCA TGCTGGTGCT CACTTCCTTC CTGTGGGACA GCCACTCCCA
    CCCTCTTTAC TGCCCCAGTA CCCCGTCTCT CAGCTGCCCC CCATCTCTCA GCTCCCCTCT
    GCTCCTCCTC ACGTGTCTGT GGCTCAGCCA GGCTTCCCAC CCCTGCCGGT CACGATGGCA
    GCTGGCGTTA GTCAGCCTCT GCTCACCTTG GCTTCATCTG CTGCGGCGGC GGCCGTCCCC
    GGGGCACCCA CTGTGGTCCC TAGTCAGCTT CCTGCCCTCC TGCAGCCTGT GACCCAGCTG
    CCGAGTCAGG CTCACCCGCA GCTCCTGCAG CCAGCAGTTC AGTCCATGGG GATCCCCGCC
    AGCCTTGGAC AGACTGCCGA GGTGCCACTT CCCCCTGGAG ATATTCTCTA CCAGGGCTTC
    CCACCTCGAC TGCCATCGCA GTACCCAGGA GACTCAAACA TTGCCCCCTC CTCCAGCGCA
    GCTTCTGTGT GCCTGCCTTC TGCAGTCCTG TCCCCTCCCA TGCCGACAGA GGCGCTGGCT
    GCCCCGGGTT ACTTTCCTGC AATGGTGCAG CCCTATGTGG AATCCAGTCT CTTGGTTCCT
    GTGGGCAGTG TAGGAGGACA GGTCCAGGTG TCCCAGCCGG CAGTGAGCTT AGCACAAGCC
    CCCACCACAT CCTCCCAGCA AGCAGCTCTG GAGAGCACTC AGGTCGTCTC TCAGGTGGCT
    CCTCCAGAGC CAGCTCCCGG GGCTCAGCCA CAGCCTGCCC AGCCAGCCGC GGTGGTCTCT
    TCTATAGACA GCGCACATTC AGATGTGGCT TCAGGCCTGA GTGATGGCAA CGAGAATGTC
    CCCTCATCCA GCGGGAGGCA CGAGGGCAGG ACCACGAAGC GGCTGCATCG GAAGTCTATA
    AGGAGCCGCT CTCGTCACGA GAAGCCTTCC CGCCCCAAAC TGAGAATTCT CAATGTTTCA
    AATAAGGGAG ATCGGGTGGT AGAATGTCAG TTAGAGACTC ACAATCGGAA GATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAACCCT GAGGAGATAG CCACAATTAT GGTGAACAAT
    GACTTTATTC TGGCACTGGA GAGAGATGCG TTTGTGGAGC AAGTGCGGGA GATCATTGAG
    AAAGCTGACG AGATGCTCAG TGAGGATGTC AGCGTGGAGC CAGAGGGTGA CCAGAGCTTG
    GAGAGTCTCC ATGGCAAGGA TGACTATGGC TTCTCAGGCA CCCAGAAATT GGAAGGAGAG
    TTCAAACAAC CAATTCCTGC ATCTTCCTTG CCACAACAGA TAGCAGGCAT TCCTGCCAGT
    TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTAAGTCC TGTGCCAGAA
    AGTCGATTAC GAGAATCAAA ACTTTTCACT GAAATTTCAG ATACGGTTGC TGCTTCCACA
    TCTGCTGGCC CTGGAATGAA CTTGTCTCAC TCTGCCTCAT CCCTTAGTCT GCAGCAGGCC
    TTTTCTGAAC TTAGACATGC TCGGATGGCA GAAGGGCCGA GTACGGCCCC TCCCCACTTT
    GGTCAGACAG GCCCCGTGTT TCCAGCAGTG CCTCCCACCC TGAGCAGTGC CCCTGGAGCC
    CCAGCCGCAG CAGCCGCAGC CTCGGTGTCA GCCCCTGCCC CAAGCCACCC TCTTAGTGAC
    GTGACCACAT CGGTCATTCA GACTGAGGTG ACAGTGGCCC CTGGAACAGG TGTCATGCCT
    CCCAGTGGTC TCCCTGTACC CCCTGTGTCT GAGTCCCTGG CACCTTCCAG CGTGGCCCCC
    AGCGTCACCA TCCCTGCAGT TCTCTCGGCA CTACCAGCGC CCCAGGCGGC ACAGGCTCCC
    ACCCCTGGCA GCGGGGCCTC CAGTGCAGGC ACTTTCCCCT CTGCACCCGC ATCCCTGACA
    CCAGCCTCTG CTGTGGGCAG CGCTGTTGCC TCTGCTGCTC AGCTTCCGCC TGTGCCGTCT
    CAGCCCGCGG TAGGCAGCTC GACCGGGGTG GCCACGCTGA CAGCCGTGCC CGCTGCCGCT
    CTGGGCCCTA GCCTCACGGC ACTGCCGTCC CTTGCTCTCA GCAGCAGTAC CTCTGCCCCA
    GCTCTGGCAG AGACAGTGCT GGTCAGTGCC CACGCACTGG ATAAGACGGC CCATAGTAGC
    ACCACTGGCC TGGCTCTGTC CCTTCCTGCC ACCTCGTCCT CTCCCGCCCC TGGAGCAGGA
    GTGTCCAGTG CTGTTTCTCA GCCTACTGCT GCGGCTCACC CTTTGGTCCT CCCATCAGTC
    GTAACCTCTA CTCCCGTACT CTCCCAGGCA GGACCCATGT CAGCACCTCT ACTGCCCCAG
    GTACCCGGTA TTCCACCCCT GGTACAACCT GTTGTCAGCG TGCCTGCTGT GCAACAGACC
    CTGGTTCATA GTCAGCCTCA GCCCGCTCCA CTCCCCAACC AGCCCCACAC TCACTGTCCC
    GAGATAGACG CCGACACTCA ACCCAAAGCT CCCGGGATCG ACGACATCAA GACTTTGGAG
    GAAAAGCTGC GATCTCTCTT TAGTGAGCAC AGTTCTTCTG GAGCCCAACA CGCCCCCGTC
    TCTCTGGAGA CCTCGCTCAC TGTAGAGACC GCTGTCACAG CAGGCCTCCC AACCACTGCT
    GTTGCACCAA GCAAGCCTCT GACTTCCACT GCCAGTACTT GCTTGCCACC AACCAGTCTG
    CCCTTAGGAG CAGCTGGCTT GTCAGTTATA CCAATGGTCA CTCCTGGTCA AGTTTCCACC
    CCAGTCAGCT CTGCGCCAGG AGTGAAACTT GAAACTGCTC CAGCAAAGCC ACCTTTAACT
    AAGCCTCCAG TGCTGCCCAT GGGCACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGCCCCCGC AGCCCCTGGG AGACCTGGAC
    GCTCAGCTGA GAAGAACCCT CAGTCCCGAG AGTGGCATGC TGACGTCTGC CGTTGGACCC
    GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG GAGACCGAAG CCCAACTTCC AAAGGATGTT
    CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT GTCCTTGCGC CCACTTCCAC AGCTGGTGTT
    CTGAAGATGG GGCGATTTCA GGTCTCAGTT GCAATGGATG ACCCCCAGAA GGAGGGTAAA
    AGTAAGTCGG AGGATGCCAA GTCTGTCCAC TTCGCATCCA GCACGTCAGA GTCCTCGGTG
    CTGTCAAGCA GCAGCCCCGA GAGCACCGTG GTGAAAGCAG AGCCCAACGG GCTGGCCGTC
    CGGGGCGCCT CTGCAGACAT GCCTGACAGC GCCCACCAGC CACCTGCCTC AGAAGCTGGG
    CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG ACCACCACAG CGAGCAGAGT GGGTCGCTTC
    TCTGTGTCCA GAACTGAAGA CAAGATGGCT GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT
    CCTTTCATGG ACTCAGAACA CGACATTCCT CCCACCGTGA CGCCAAAGAA AGAGAAACCC
    GAACTGTCTG AGCCTTCGCA TCTGAACGGG CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC
    AGCCGGGACG TGGATGCTGG CTCAGGGAGC CCTCACTCCC CTCCGCAGCT GAGCTCCAAG
    AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC CTCAGCAATT CCTTCAACTC TTCCTACATG
    AGCAGCGACA ATGAGTCAGA CATTGAAGAC GAGGACTTAA AGTTAGAGCT TCGCCGACTA
    CGGGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA CGAAATTGAG
    TCTTTATACA CCAAACTGGG CAAGGTTCCT CCTGCTGTCA TCATTCCCCC AGCAGCCCCC
    CTTGCAGGGA GAAGGAGGCG ACCCACCAAA AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC
    TCTTTGGGGA ATAAAAGCCC TGGACCAGGT ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC
    TTGCACCCTC AGCAGACCCT CCCCGCTCCT GGCAGCGTCC CGGAGACAGG GCCCAACCAG
    CTGTTACAGC CTGTGAAGCC ATCTCCTTCC AGTGACAACC TGTACTCGGC CTTTACCAGC
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCGCCAGGTC AAGGCTGTGC CAAGTTTAAC
    TGTGCGTCCG AGCAGGTGAC GTTCAAAGCT GGCGGCAGGA GGACGCGATT TCTGAGTACG
    CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CTTGCAACCA GCTCTGTATG
    TGCCCTCCAG CAGAGCCGAA ATCAGGGACC AGCAGCACCA ACGCCGTTGG GGGAGCCGTG
    AGCAGCCAGG CCGCCCCGGC TCCACCTCCT GCCGTGACGT CCAGCAGGAA GGGCATGTTC
    ACGGACGACC TGCACAAGCT GGTGGACAAC TGGGCCCGGG ACGCCATGAG TCTCTCAGGC
    AGGCGGGGAA GCAAAGGACA CGTGAGCTAT GAGGGCCCTG GCATGGCAAG GAAGTTCTCT
    GCGCCCGGTC AGCTGTGCGT CTCCATGACC TCAAACCTGG GCGGCTCTGC CCCCGGCTCT
    GCAGCGTCAG CTACCTCTCT AGGGCACTTC CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC
    GGTTTCCCTA CCCCCCCGTT TGGCACGCAG TGGAGCGGGA CGGGTGGCCC GGCACCCCAG
    CCGCTTAGCC AGTTCCAGCC TGTGGGAACA GCCTCCTTAC AGAATTTCAA CATCAGCAAC
    TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89988
    Clone ID Related Accession (Same CDS sequence) XM_015463543.1 , XM_015471359.1
    Accession Version XM_015471359.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGATTTTA CAAAGAGAGA TTTTTGCTCA GTGTTTGTCT TCGTAAATTC TCATTGCTGC 
    TGCTGCTCTC AAAAGGATTG TATAAATGAG GGGGTGAAGC CAGCCAGTTT TGACAAAGTA
    GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAC GGCAAAACAA AGATGAAAGA
    TACTCTATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTACGGGTA
    GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AATTATGGCT ACGTATTGAA
    GATATTAAGA AACTAAAGGG GAAATACAAA GACAATGAAG CTATCGAGTT TTCCTTTGAC
    TTGGAGAAAG ATGTGCCAGA AGACGTTGCT CAAGAAATGG TAGATTCTGG GTATGTCTGT
    GAAGGTGATC ACAAGACCAT GGCAAAAGCC ATCAAAGATA GAGTGTCGTT AATTAAGAGA
    AAACGAGAAC AGCGTCAATT AGTGCGGGAG GAGCAAGAAA AAAGAAAGCA GGAGGAGAGC
    AGCTTCAAGC AGCAGGTGGA GCAGCAGCCC AGCACTGCCC AGGCCGGAGT CCAGCAGCCC
    CCTGCCACCA GCACTGGTGG ACCCGCTGCC TCTGCTGCGT CTGCGTCCGT CTCCACGCAG
    GTAGAGCCAG AAGAGCCTGA GGCCGACCAG CACCAGCAGC TGCAGTACCA GCAGCCTGGT
    GTGTCTGTGA TATCTGACGG GACGGTTGAC AGTGGTCAGG GGTCTTCTGT CTTCACGGAA
    TCTCGAGTCA GCAGCCAACA GACAGTTTCG TACGGGTCCC AGCATGAGCA GGCACCTTCC
    ACTGGCGCCC TCCCAGGGCA CACAGCATCT GTTGTCCAAA CGCAGGCTCA GCCCCATGCG
    GTATATCCAC CCTCAAGTTT GCCTCAGTCC ATGGCGCATC CGCGTGGGGG GACCCCAACA
    TACCCAGAAT CACAGATATT TTTCCCAACT ATTCATGAAC GTCCAGTTTC TTTTTCACCA
    CCTCCCACCT GTCCACCGAA GGTGGCCATT GCCCAGCGGC GTAAGAGCAC CTCCTTCTTG
    GAAGCCCAAA CTCACCAGTT CCAACCCCTG CTGAGGACTG TCGGGCAGAG TCTTCTTCCA
    CCTGGTGGCA GCCCACCTAA CTGGACTCCA GAGACTGTAG TTATGTGGGG CACTGCAGCC
    AGTAGAGTTG CTGGAGAGCT GTGTGAGGTG CAGGCCCATC CTGTGTTCGA GCCAGCTCCA
    GTTTACAGTG ACTACAGAGC TGGACTGATG CTTCCAGAAG AAGCTCACTA CTTCCTCCCT
    CCAGAAGCAG TGTATCTAGC CGGGGTACAT TACCAGGCCC AGGTGGCAGA ACAGTTTGAG
    GGTTTTCCAT ACAGCTCACC AGTCCTGTCA AGTCCTATGA AGCAGACACC TGAGCAGAAG
    CCAGTGCACG GGGGCCCCAC CTCCAGTTCT GTCTTTGAAT TTCCATCGGG ACAGGCTTTC
    CTGGTAGGAC ACCTCCAGAA TGTAAGATTA GATTCGGGAC TGAGCCCAGG CTCTCCCCAG
    TCTAGCGTTT CTGCACCTAT CAGTACAGAT GCCACACGCT TGAAGTTTCA CCCTGTCTTT
    GTTCCTCATT CTGCACCTGC TGTGTTAACT CATAACAACG AGAGCAGAAG CAGTTGTGTG
    TTTGAGTTTC ACGTTCATAC TCCAAGCTCC TCTTCAGGAG AAGGAGGGGG AGGAGTTTTA
    CCTCAGCGCA TTTACCGAAA TCGGCAGGTT GCTGTGGACT CGAACCAGGA AGAACCACCT
    CTTCAACCTG CAGGACTACA CAGCCGCCTG CAGCCTGTGA CTGAAGAACA GCAGATGCCC
    CAGGCCCCAG AGTTGACCAT CTCTGTGGTG GAGCCCACCG GACAGACCTG GCCCATAGGA
    AGCCCAGAGT ACTCCAGTGA TTCCTCCCAG GTCACTTCTT CAGACCCCAG TGAATTTCAG
    TCACCTCCCC CTACAGGGGG AGCAGCTGCT CCTTTTGGCT CTGACGTCTC ATTACCCTTT
    ATCCGCGTGC CTCAGACAGT GTTACAAGAA TCCCCACTCT TCTTCTGTTT CCCCCAAGGA
    GCCACCTCTC CGCAGGTTTT ATCCGCCTCA TTTTCTTCAG GAGGATCTGC ACTCCATCCA
    CAGGCACAGG GGCAGAGCCA GGGTCAGCCA TCTTCAAGTA GTTTAACAGG GGTTCCATCC
    TCCCAACCCA TGCAGCATTC TCAGCAGCAG CAAGGAGTGC AGCCAACAGC CCCCTCTCAG
    CAGGCGGTGC AGTATCCGCT CCCTCAGACA TCGGCCCCCA GCGAGGCCAC GATTGCACAG
    CCTGTGAGTC AGCCTCCACC TCCACAGGCC TTGCCACACG TGTCCGCGGG AAAACAGCTT
    CCAGTTTCCC AGCCAGTACC AACTCTCCAA GGCGAACCTC AGATCCCAGT TGCAACACAG
    CCCTCAGTCG TTCCCGTCCA TGCTGGTGCT CACTTCCTTC CTGTGGGACA GCCACTCCCA
    CCCTCTTTAC TGCCCCAGTA CCCCGTCTCT CAGCTGCCCC CCATCTCTCA GCTCCCCTCT
    GCTCCTCCTC ACGTGTCTGT GGCTCAGCCA GGCTTCCCAC CCCTGCCGGT CACGATGGCA
    GCTGGCGTTA GTCAGCCTCT GCTCACCTTG GCTTCATCTG CTGCGGCGGC GGCCGTCCCC
    GGGGCACCCA CTGTGGTCCC TAGTCAGCTT CCTGCCCTCC TGCAGCCTGT GACCCAGCTG
    CCGAGTCAGG CTCACCCGCA GCTCCTGCAG CCAGCAGTTC AGTCCATGGG GATCCCCGCC
    AGCCTTGGAC AGACTGCCGA GGTGCCACTT CCCCCTGGAG ATATTCTCTA CCAGGGCTTC
    CCACCTCGAC TGCCATCGCA GTACCCAGGA GACTCAAACA TTGCCCCCTC CTCCAGCGCA
    GCTTCTGTGT GCCTGCCTTC TGCAGTCCTG TCCCCTCCCA TGCCGACAGA GGCGCTGGCT
    GCCCCGGGTT ACTTTCCTGC AATGGTGCAG CCCTATGTGG AATCCAGTCT CTTGGTTCCT
    GTGGGCAGTG TAGGAGGACA GGTCCAGGTG TCCCAGCCGG CAGTGAGCTT AGCACAAGCC
    CCCACCACAT CCTCCCAGCA AGCAGCTCTG GAGAGCACTC AGGTCGTCTC TCAGGTGGCT
    CCTCCAGAGC CAGCTCCCGG GGCTCAGCCA CAGCCTGCCC AGCCAGCCGC GGTGGTCTCT
    TCTATAGACA GCGCACATTC AGATGTGGCT TCAGGCCTGA GTGATGGCAA CGAGAATGTC
    CCCTCATCCA GCGGGAGGCA CGAGGGCAGG ACCACGAAGC GGCTGCATCG GAAGTCTATA
    AGGAGCCGCT CTCGTCACGA GAAGCCTTCC CGCCCCAAAC TGAGAATTCT CAATGTTTCA
    AATAAGGGAG ATCGGGTGGT AGAATGTCAG TTAGAGACTC ACAATCGGAA GATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAACCCT GAGGAGATAG CCACAATTAT GGTGAACAAT
    GACTTTATTC TGGCACTGGA GAGAGATGCG TTTGTGGAGC AAGTGCGGGA GATCATTGAG
    AAAGCTGACG AGATGCTCAG TGAGGATGTC AGCGTGGAGC CAGAGGGTGA CCAGAGCTTG
    GAGAGTCTCC ATGGCAAGGA TGACTATGGC TTCTCAGGCA CCCAGAAATT GGAAGGAGAG
    TTCAAACAAC CAATTCCTGC ATCTTCCTTG CCACAACAGA TAGCAGGCAT TCCTGCCAGT
    TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTAAGTCC TGTGCCAGAA
    AGTCGATTAC GAGAATCAAA ACTTTTCACT GAAATTTCAG ATACGGTTGC TGCTTCCACA
    TCTGCTGGCC CTGGAATGAA CTTGTCTCAC TCTGCCTCAT CCCTTAGTCT GCAGCAGGCC
    TTTTCTGAAC TTAGACATGC TCGGATGGCA GAAGGGCCGA GTACGGCCCC TCCCCACTTT
    GGTCAGACAG GCCCCGTGTT TCCAGCAGTG CCTCCCACCC TGAGCAGTGC CCCTGGAGCC
    CCAGCCGCAG CAGCCGCAGC CTCGGTGTCA GCCCCTGCCC CAAGCCACCC TCTTAGTGAC
    GTGACCACAT CGGTCATTCA GACTGAGGTG ACAGTGGCCC CTGGAACAGG TGTCATGCCT
    CCCAGTGGTC TCCCTGTACC CCCTGTGTCT GAGTCCCTGG CACCTTCCAG CGTGGCCCCC
    AGCGTCACCA TCCCTGCAGT TCTCTCGGCA CTACCAGCGC CCCAGGCGGC ACAGGCTCCC
    ACCCCTGGCA GCGGGGCCTC CAGTGCAGGC ACTTTCCCCT CTGCACCCGC ATCCCTGACA
    CCAGCCTCTG CTGTGGGCAG CGCTGTTGCC TCTGCTGCTC AGCTTCCGCC TGTGCCGTCT
    CAGCCCGCGG TAGGCAGCTC GACCGGGGTG GCCACGCTGA CAGCCGTGCC CGCTGCCGCT
    CTGGGCCCTA GCCTCACGGC ACTGCCGTCC CTTGCTCTCA GCAGCAGTAC CTCTGCCCCA
    GCTCTGGCAG AGACAGTGCT GGTCAGTGCC CACGCACTGG ATAAGACGGC CCATAGTAGC
    ACCACTGGCC TGGCTCTGTC CCTTCCTGCC ACCTCGTCCT CTCCCGCCCC TGGAGCAGGA
    GTGTCCAGTG CTGTTTCTCA GCCTACTGCT GCGGCTCACC CTTTGGTCCT CCCATCAGTC
    GTAACCTCTA CTCCCGTACT CTCCCAGGCA GGACCCATGT CAGCACCTCT ACTGCCCCAG
    GTACCCGGTA TTCCACCCCT GGTACAACCT GTTGTCAGCG TGCCTGCTGT GCAACAGACC
    CTGGTTCATA GTCAGCCTCA GCCCGCTCCA CTCCCCAACC AGCCCCACAC TCACTGTCCC
    GAGATAGACG CCGACACTCA ACCCAAAGCT CCCGGGATCG ACGACATCAA GACTTTGGAG
    GAAAAGCTGC GATCTCTCTT TAGTGAGCAC AGTTCTTCTG GAGCCCAACA CGCCCCCGTC
    TCTCTGGAGA CCTCGCTCAC TGTAGAGACC GCTGTCACAG CAGGCCTCCC AACCACTGCT
    GTTGCACCAA GCAAGCCTCT GACTTCCACT GCCAGTACTT GCTTGCCACC AACCAGTCTG
    CCCTTAGGAG CAGCTGGCTT GTCAGTTATA CCAATGGTCA CTCCTGGTCA AGTTTCCACC
    CCAGTCAGCT CTGCGCCAGG AGTGAAACTT GAAACTGCTC CAGCAAAGCC ACCTTTAACT
    AAGCCTCCAG TGCTGCCCAT GGGCACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGCCCCCGC AGCCCCTGGG AGACCTGGAC
    GCTCAGCTGA GAAGAACCCT CAGTCCCGAG AGTGGCATGC TGACGTCTGC CGTTGGACCC
    GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG GAGACCGAAG CCCAACTTCC AAAGGATGTT
    CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT GTCCTTGCGC CCACTTCCAC AGCTGGTGTT
    CTGAAGATGG GGCGATTTCA GGTCTCAGTT GCAATGGATG ACCCCCAGAA GGAGGGTAAA
    AGTAAGTCGG AGGATGCCAA GTCTGTCCAC TTCGCATCCA GCACGTCAGA GTCCTCGGTG
    CTGTCAAGCA GCAGCCCCGA GAGCACCGTG GTGAAAGCAG AGCCCAACGG GCTGGCCGTC
    CGGGGCGCCT CTGCAGACAT GCCTGACAGC GCCCACCAGC CACCTGCCTC AGAAGCTGGG
    CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG ACCACCACAG CGAGCAGAGT GGGTCGCTTC
    TCTGTGTCCA GAACTGAAGA CAAGATGGCT GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT
    CCTTTCATGG ACTCAGAACA CGACATTCCT CCCACCGTGA CGCCAAAGAA AGAGAAACCC
    GAACTGTCTG AGCCTTCGCA TCTGAACGGG CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC
    AGCCGGGACG TGGATGCTGG CTCAGGGAGC CCTCACTCCC CTCCGCAGCT GAGCTCCAAG
    AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC CTCAGCAATT CCTTCAACTC TTCCTACATG
    AGCAGCGACA ATGAGTCAGA CATTGAAGAC GAGGACTTAA AGTTAGAGCT TCGCCGACTA
    CGGGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA CGAAATTGAG
    TCTTTATACA CCAAACTGGG CAAGGTTCCT CCTGCTGTCA TCATTCCCCC AGCAGCCCCC
    CTTGCAGGGA GAAGGAGGCG ACCCACCAAA AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC
    TCTTTGGGGA ATAAAAGCCC TGGACCAGGT ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC
    TTGCACCCTC AGCAGACCCT CCCCGCTCCT GGCAGCGTCC CGGAGACAGG GCCCAACCAG
    CTGTTACAGC CTGTGAAGCC ATCTCCTTCC AGTGACAACC TGTACTCGGC CTTTACCAGC
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCGCCAGGTC AAGGCTGTGC CAAGTTTAAC
    TGTGCGTCCG AGCAGGTGAC GTTCAAAGCT GGCGGCAGGA GGACGCGATT TCTGAGTACG
    CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CTTGCAACCA GCTCTGTATG
    TGCCCTCCAG CAGAGCCGAA ATCAGGGACC AGCAGCACCA ACGCCGTTGG GGGAGCCGTG
    AGCAGCCAGG CCGCCCCGGC TCCACCTCCT GCCGTGACGT CCAGCAGGAA GGGCATGTTC
    ACGGACGACC TGCACAAGCT GGTGGACAAC TGGGCCCGGG ACGCCATGAG TCTCTCAGGC
    AGGCGGGGAA GCAAAGGACA CGTGAGCTAT GAGGGCCCTG GCATGGCAAG GAAGTTCTCT
    GCGCCCGGTC AGCTGTGCGT CTCCATGACC TCAAACCTGG GCGGCTCTGC CCCCGGCTCT
    GCAGCGTCAG CTACCTCTCT AGGGCACTTC CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC
    GGTTTCCCTA CCCCCCCGTT TGGCACGCAG TGGAGCGGGA CGGGTGGCCC GGCACCCCAG
    CCGCTTAGCC AGTTCCAGCC TGTGGGAACA GCCTCCTTAC AGAATTTCAA CATCAGCAAC
    TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326845.1
    CDS311..7621
    Translation

    Target ORF information:

    RefSeq Version XM_015471359.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGATTTTA CAAAGAGAGA TTTTTGCTCA GTGTTTGTCT TCGTAAATTC TCATTGCTGC 
    TGCTGCTCTC AAAAGGATTG TATAAATGAG GGGGTGAAGC CAGCCAGTTT TGACAAAGTA
    GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAC GGCAAAACAA AGATGAAAGA
    TACTCTATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTACGGGTA
    GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AATTATGGCT ACGTATTGAA
    GATATTAAGA AACTAAAGGG GAAATACAAA GACAATGAAG CTATCGAGTT TTCCTTTGAC
    TTGGAGAAAG ATGTGCCAGA AGACGTTGCT CAAGAAATGG TAGATTCTGG GTATGTCTGT
    GAAGGTGATC ACAAGACCAT GGCAAAAGCC ATCAAAGATA GAGTGTCGTT AATTAAGAGA
    AAACGAGAAC AGCGTCAATT AGTGCGGGAG GAGCAAGAAA AAAGAAAGCA GGAGGAGAGC
    AGCTTCAAGC AGCAGGTGGA GCAGCAGCCC AGCACTGCCC AGGCCGGAGT CCAGCAGCCC
    CCTGCCACCA GCACTGGTGG ACCCGCTGCC TCTGCTGCGT CTGCGTCCGT CTCCACGCAG
    GTAGAGCCAG AAGAGCCTGA GGCCGACCAG CACCAGCAGC TGCAGTACCA GCAGCCTGGT
    GTGTCTGTGA TATCTGACGG GACGGTTGAC AGTGGTCAGG GGTCTTCTGT CTTCACGGAA
    TCTCGAGTCA GCAGCCAACA GACAGTTTCG TACGGGTCCC AGCATGAGCA GGCACCTTCC
    ACTGGCGCCC TCCCAGGGCA CACAGCATCT GTTGTCCAAA CGCAGGCTCA GCCCCATGCG
    GTATATCCAC CCTCAAGTTT GCCTCAGTCC ATGGCGCATC CGCGTGGGGG GACCCCAACA
    TACCCAGAAT CACAGATATT TTTCCCAACT ATTCATGAAC GTCCAGTTTC TTTTTCACCA
    CCTCCCACCT GTCCACCGAA GGTGGCCATT GCCCAGCGGC GTAAGAGCAC CTCCTTCTTG
    GAAGCCCAAA CTCACCAGTT CCAACCCCTG CTGAGGACTG TCGGGCAGAG TCTTCTTCCA
    CCTGGTGGCA GCCCACCTAA CTGGACTCCA GAGACTGTAG TTATGTGGGG CACTGCAGCC
    AGTAGAGTTG CTGGAGAGCT GTGTGAGGTG CAGGCCCATC CTGTGTTCGA GCCAGCTCCA
    GTTTACAGTG ACTACAGAGC TGGACTGATG CTTCCAGAAG AAGCTCACTA CTTCCTCCCT
    CCAGAAGCAG TGTATCTAGC CGGGGTACAT TACCAGGCCC AGGTGGCAGA ACAGTTTGAG
    GGTTTTCCAT ACAGCTCACC AGTCCTGTCA AGTCCTATGA AGCAGACACC TGAGCAGAAG
    CCAGTGCACG GGGGCCCCAC CTCCAGTTCT GTCTTTGAAT TTCCATCGGG ACAGGCTTTC
    CTGGTAGGAC ACCTCCAGAA TGTAAGATTA GATTCGGGAC TGAGCCCAGG CTCTCCCCAG
    TCTAGCGTTT CTGCACCTAT CAGTACAGAT GCCACACGCT TGAAGTTTCA CCCTGTCTTT
    GTTCCTCATT CTGCACCTGC TGTGTTAACT CATAACAACG AGAGCAGAAG CAGTTGTGTG
    TTTGAGTTTC ACGTTCATAC TCCAAGCTCC TCTTCAGGAG AAGGAGGGGG AGGAGTTTTA
    CCTCAGCGCA TTTACCGAAA TCGGCAGGTT GCTGTGGACT CGAACCAGGA AGAACCACCT
    CTTCAACCTG CAGGACTACA CAGCCGCCTG CAGCCTGTGA CTGAAGAACA GCAGATGCCC
    CAGGCCCCAG AGTTGACCAT CTCTGTGGTG GAGCCCACCG GACAGACCTG GCCCATAGGA
    AGCCCAGAGT ACTCCAGTGA TTCCTCCCAG GTCACTTCTT CAGACCCCAG TGAATTTCAG
    TCACCTCCCC CTACAGGGGG AGCAGCTGCT CCTTTTGGCT CTGACGTCTC ATTACCCTTT
    ATCCGCGTGC CTCAGACAGT GTTACAAGAA TCCCCACTCT TCTTCTGTTT CCCCCAAGGA
    GCCACCTCTC CGCAGGTTTT ATCCGCCTCA TTTTCTTCAG GAGGATCTGC ACTCCATCCA
    CAGGCACAGG GGCAGAGCCA GGGTCAGCCA TCTTCAAGTA GTTTAACAGG GGTTCCATCC
    TCCCAACCCA TGCAGCATTC TCAGCAGCAG CAAGGAGTGC AGCCAACAGC CCCCTCTCAG
    CAGGCGGTGC AGTATCCGCT CCCTCAGACA TCGGCCCCCA GCGAGGCCAC GATTGCACAG
    CCTGTGAGTC AGCCTCCACC TCCACAGGCC TTGCCACACG TGTCCGCGGG AAAACAGCTT
    CCAGTTTCCC AGCCAGTACC AACTCTCCAA GGCGAACCTC AGATCCCAGT TGCAACACAG
    CCCTCAGTCG TTCCCGTCCA TGCTGGTGCT CACTTCCTTC CTGTGGGACA GCCACTCCCA
    CCCTCTTTAC TGCCCCAGTA CCCCGTCTCT CAGCTGCCCC CCATCTCTCA GCTCCCCTCT
    GCTCCTCCTC ACGTGTCTGT GGCTCAGCCA GGCTTCCCAC CCCTGCCGGT CACGATGGCA
    GCTGGCGTTA GTCAGCCTCT GCTCACCTTG GCTTCATCTG CTGCGGCGGC GGCCGTCCCC
    GGGGCACCCA CTGTGGTCCC TAGTCAGCTT CCTGCCCTCC TGCAGCCTGT GACCCAGCTG
    CCGAGTCAGG CTCACCCGCA GCTCCTGCAG CCAGCAGTTC AGTCCATGGG GATCCCCGCC
    AGCCTTGGAC AGACTGCCGA GGTGCCACTT CCCCCTGGAG ATATTCTCTA CCAGGGCTTC
    CCACCTCGAC TGCCATCGCA GTACCCAGGA GACTCAAACA TTGCCCCCTC CTCCAGCGCA
    GCTTCTGTGT GCCTGCCTTC TGCAGTCCTG TCCCCTCCCA TGCCGACAGA GGCGCTGGCT
    GCCCCGGGTT ACTTTCCTGC AATGGTGCAG CCCTATGTGG AATCCAGTCT CTTGGTTCCT
    GTGGGCAGTG TAGGAGGACA GGTCCAGGTG TCCCAGCCGG CAGTGAGCTT AGCACAAGCC
    CCCACCACAT CCTCCCAGCA AGCAGCTCTG GAGAGCACTC AGGTCGTCTC TCAGGTGGCT
    CCTCCAGAGC CAGCTCCCGG GGCTCAGCCA CAGCCTGCCC AGCCAGCCGC GGTGGTCTCT
    TCTATAGACA GCGCACATTC AGATGTGGCT TCAGGCCTGA GTGATGGCAA CGAGAATGTC
    CCCTCATCCA GCGGGAGGCA CGAGGGCAGG ACCACGAAGC GGCTGCATCG GAAGTCTATA
    AGGAGCCGCT CTCGTCACGA GAAGCCTTCC CGCCCCAAAC TGAGAATTCT CAATGTTTCA
    AATAAGGGAG ATCGGGTGGT AGAATGTCAG TTAGAGACTC ACAATCGGAA GATGGTTACA
    TTCAAATTTG ATTTAGATGG TGACAACCCT GAGGAGATAG CCACAATTAT GGTGAACAAT
    GACTTTATTC TGGCACTGGA GAGAGATGCG TTTGTGGAGC AAGTGCGGGA GATCATTGAG
    AAAGCTGACG AGATGCTCAG TGAGGATGTC AGCGTGGAGC CAGAGGGTGA CCAGAGCTTG
    GAGAGTCTCC ATGGCAAGGA TGACTATGGC TTCTCAGGCA CCCAGAAATT GGAAGGAGAG
    TTCAAACAAC CAATTCCTGC ATCTTCCTTG CCACAACAGA TAGCAGGCAT TCCTGCCAGT
    TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTAAGTCC TGTGCCAGAA
    AGTCGATTAC GAGAATCAAA ACTTTTCACT GAAATTTCAG ATACGGTTGC TGCTTCCACA
    TCTGCTGGCC CTGGAATGAA CTTGTCTCAC TCTGCCTCAT CCCTTAGTCT GCAGCAGGCC
    TTTTCTGAAC TTAGACATGC TCGGATGGCA GAAGGGCCGA GTACGGCCCC TCCCCACTTT
    GGTCAGACAG GCCCCGTGTT TCCAGCAGTG CCTCCCACCC TGAGCAGTGC CCCTGGAGCC
    CCAGCCGCAG CAGCCGCAGC CTCGGTGTCA GCCCCTGCCC CAAGCCACCC TCTTAGTGAC
    GTGACCACAT CGGTCATTCA GACTGAGGTG ACAGTGGCCC CTGGAACAGG TGTCATGCCT
    CCCAGTGGTC TCCCTGTACC CCCTGTGTCT GAGTCCCTGG CACCTTCCAG CGTGGCCCCC
    AGCGTCACCA TCCCTGCAGT TCTCTCGGCA CTACCAGCGC CCCAGGCGGC ACAGGCTCCC
    ACCCCTGGCA GCGGGGCCTC CAGTGCAGGC ACTTTCCCCT CTGCACCCGC ATCCCTGACA
    CCAGCCTCTG CTGTGGGCAG CGCTGTTGCC TCTGCTGCTC AGCTTCCGCC TGTGCCGTCT
    CAGCCCGCGG TAGGCAGCTC GACCGGGGTG GCCACGCTGA CAGCCGTGCC CGCTGCCGCT
    CTGGGCCCTA GCCTCACGGC ACTGCCGTCC CTTGCTCTCA GCAGCAGTAC CTCTGCCCCA
    GCTCTGGCAG AGACAGTGCT GGTCAGTGCC CACGCACTGG ATAAGACGGC CCATAGTAGC
    ACCACTGGCC TGGCTCTGTC CCTTCCTGCC ACCTCGTCCT CTCCCGCCCC TGGAGCAGGA
    GTGTCCAGTG CTGTTTCTCA GCCTACTGCT GCGGCTCACC CTTTGGTCCT CCCATCAGTC
    GTAACCTCTA CTCCCGTACT CTCCCAGGCA GGACCCATGT CAGCACCTCT ACTGCCCCAG
    GTACCCGGTA TTCCACCCCT GGTACAACCT GTTGTCAGCG TGCCTGCTGT GCAACAGACC
    CTGGTTCATA GTCAGCCTCA GCCCGCTCCA CTCCCCAACC AGCCCCACAC TCACTGTCCC
    GAGATAGACG CCGACACTCA ACCCAAAGCT CCCGGGATCG ACGACATCAA GACTTTGGAG
    GAAAAGCTGC GATCTCTCTT TAGTGAGCAC AGTTCTTCTG GAGCCCAACA CGCCCCCGTC
    TCTCTGGAGA CCTCGCTCAC TGTAGAGACC GCTGTCACAG CAGGCCTCCC AACCACTGCT
    GTTGCACCAA GCAAGCCTCT GACTTCCACT GCCAGTACTT GCTTGCCACC AACCAGTCTG
    CCCTTAGGAG CAGCTGGCTT GTCAGTTATA CCAATGGTCA CTCCTGGTCA AGTTTCCACC
    CCAGTCAGCT CTGCGCCAGG AGTGAAACTT GAAACTGCTC CAGCAAAGCC ACCTTTAACT
    AAGCCTCCAG TGCTGCCCAT GGGCACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG
    CTGCCACCTT TTCCAGGACC TTCACTAACT CAGCCCCCGC AGCCCCTGGG AGACCTGGAC
    GCTCAGCTGA GAAGAACCCT CAGTCCCGAG AGTGGCATGC TGACGTCTGC CGTTGGACCC
    GTGTCTGTGG TGGCTCCAAC AGCAGTTGTG GAGACCGAAG CCCAACTTCC AAAGGATGTT
    CTTTCAGTCT CTCAAGTCAC AGAAGCTCCT GTCCTTGCGC CCACTTCCAC AGCTGGTGTT
    CTGAAGATGG GGCGATTTCA GGTCTCAGTT GCAATGGATG ACCCCCAGAA GGAGGGTAAA
    AGTAAGTCGG AGGATGCCAA GTCTGTCCAC TTCGCATCCA GCACGTCAGA GTCCTCGGTG
    CTGTCAAGCA GCAGCCCCGA GAGCACCGTG GTGAAAGCAG AGCCCAACGG GCTGGCCGTC
    CGGGGCGCCT CTGCAGACAT GCCTGACAGC GCCCACCAGC CACCTGCCTC AGAAGCTGGG
    CAGCCTAGCA AGGTGGGCCG TTTTCAGGTG ACCACCACAG CGAGCAGAGT GGGTCGCTTC
    TCTGTGTCCA GAACTGAAGA CAAGATGGCT GAGGTGAAGG GAGAGGAGCC AGTGGTCTCT
    CCTTTCATGG ACTCAGAACA CGACATTCCT CCCACCGTGA CGCCAAAGAA AGAGAAACCC
    GAACTGTCTG AGCCTTCGCA TCTGAACGGG CCGTCTTCCG ACCTGGAGGC TGCCTTCCTC
    AGCCGGGACG TGGATGCTGG CTCAGGGAGC CCTCACTCCC CTCCGCAGCT GAGCTCCAAG
    AGCCTTCCTG CCCAGAGCCT CAGTCAGAGC CTCAGCAATT CCTTCAACTC TTCCTACATG
    AGCAGCGACA ATGAGTCAGA CATTGAAGAC GAGGACTTAA AGTTAGAGCT TCGCCGACTA
    CGGGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA CGAAATTGAG
    TCTTTATACA CCAAACTGGG CAAGGTTCCT CCTGCTGTCA TCATTCCCCC AGCAGCCCCC
    CTTGCAGGGA GAAGGAGGCG ACCCACCAAA AGCAAAGGGA GCAAGTCCAG TCGCAGCAGC
    TCTTTGGGGA ATAAAAGCCC TGGACCAGGT ACCCTGTCTG GCCAGAGTGC AGCTTCAGTC
    TTGCACCCTC AGCAGACCCT CCCCGCTCCT GGCAGCGTCC CGGAGACAGG GCCCAACCAG
    CTGTTACAGC CTGTGAAGCC ATCTCCTTCC AGTGACAACC TGTACTCGGC CTTTACCAGC
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCGCCAGGTC AAGGCTGTGC CAAGTTTAAC
    TGTGCGTCCG AGCAGGTGAC GTTCAAAGCT GGCGGCAGGA GGACGCGATT TCTGAGTACG
    CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CTTGCAACCA GCTCTGTATG
    TGCCCTCCAG CAGAGCCGAA ATCAGGGACC AGCAGCACCA ACGCCGTTGG GGGAGCCGTG
    AGCAGCCAGG CCGCCCCGGC TCCACCTCCT GCCGTGACGT CCAGCAGGAA GGGCATGTTC
    ACGGACGACC TGCACAAGCT GGTGGACAAC TGGGCCCGGG ACGCCATGAG TCTCTCAGGC
    AGGCGGGGAA GCAAAGGACA CGTGAGCTAT GAGGGCCCTG GCATGGCAAG GAAGTTCTCT
    GCGCCCGGTC AGCTGTGCGT CTCCATGACC TCAAACCTGG GCGGCTCTGC CCCCGGCTCT
    GCAGCGTCAG CTACCTCTCT AGGGCACTTC CCCAAGTCCA TGTGCCCCCC TCAGCAGTAC
    GGTTTCCCTA CCCCCCCGTT TGGCACGCAG TGGAGCGGGA CGGGTGGCCC GGCACCCCAG
    CCGCTTAGCC AGTTCCAGCC TGTGGGAACA GCCTCCTTAC AGAATTTCAA CATCAGCAAC
    TTGCAGAAAT CCATCAGCAA CCCCCCAGGT TCCAACCTGC GGACCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19898
    Clone ID Related Accession (Same CDS sequence) XM_005207280.3 , XM_015463526.1
    Accession Version XM_005207280.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCCT
    CAGTCCATGG CGCATCCGCG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC
    CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG
    GCCATTGCCC AGCGGCGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCA CCAGTTCCAA
    CCCCTGCTGA GGACTGTCGG GCAGAGTCTT CTTCCACCTG GTGGCAGCCC ACCTAACTGG
    ACTCCAGAGA CTGTAGTTAT GTGGGGCACT GCAGCCAGTA GAGTTGCTGG AGAGCTGTGT
    GAGGTGCAGG CCCATCCTGT GTTCGAGCCA GCTCCAGTTT ACAGTGACTA CAGAGCTGGA
    CTGATGCTTC CAGAAGAAGC TCACTACTTC CTCCCTCCAG AAGCAGTGTA TCTAGCCGGG
    GTACATTACC AGGCCCAGGT GGCAGAACAG TTTGAGGGTT TTCCATACAG CTCACCAGTC
    CTGTCAAGTC CTATGAAGCA GACACCTGAG CAGAAGCCAG TGCACGGGGG CCCCACCTCC
    AGTTCTGTCT TTGAATTTCC ATCGGGACAG GCTTTCCTGG TAGGACACCT CCAGAATGTA
    AGATTAGATT CGGGACTGAG CCCAGGCTCT CCCCAGTCTA GCGTTTCTGC ACCTATCAGT
    ACAGATGCCA CACGCTTGAA GTTTCACCCT GTCTTTGTTC CTCATTCTGC ACCTGCTGTG
    TTAACTCATA ACAACGAGAG CAGAAGCAGT TGTGTGTTTG AGTTTCACGT TCATACTCCA
    AGCTCCTCTT CAGGAGAAGG AGGGGGAGGA GTTTTACCTC AGCGCATTTA CCGAAATCGG
    CAGGTTGCTG TGGACTCGAA CCAGGAAGAA CCACCTCTTC AACCTGCAGG ACTACACAGC
    CGCCTGCAGC CTGTGACTGA AGAACAGCAG ATGCCCCAGG CCCCAGAGTT GACCATCTCT
    GTGGTGGAGC CCACCGGACA GACCTGGCCC ATAGGAAGCC CAGAGTACTC CAGTGATTCC
    TCCCAGGTCA CTTCTTCAGA CCCCAGTGAA TTTCAGTCAC CTCCCCCTAC AGGGGGAGCA
    GCTGCTCCTT TTGGCTCTGA CGTCTCATTA CCCTTTATCC GCGTGCCTCA GACAGTGTTA
    CAAGAATCCC CACTCTTCTT CTGTTTCCCC CAAGGAGCCA CCTCTCCGCA GGTTTTATCC
    GCCTCATTTT CTTCAGGAGG ATCTGCACTC CATCCACAGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCTT CAAGTAGTTT AACAGGGGTT CCATCCTCCC AACCCATGCA GCATTCTCAG
    CAGCAGCAAG GAGTGCAGCC AACAGCCCCC TCTCAGCAGG CGGTGCAGTA TCCGCTCCCT
    CAGACATCGG CCCCCAGCGA GGCCACGATT GCACAGCCTG TGAGTCAGCC TCCACCTCCA
    CAGGCCTTGC CACACGTGTC CGCGGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT
    CTCCAAGGCG AACCTCAGAT CCCAGTTGCA ACACAGCCCT CAGTCGTTCC CGTCCATGCT
    GGTGCTCACT TCCTTCCTGT GGGACAGCCA CTCCCACCCT CTTTACTGCC CCAGTACCCC
    GTCTCTCAGC TGCCCCCCAT CTCTCAGCTC CCCTCTGCTC CTCCTCACGT GTCTGTGGCT
    CAGCCAGGCT TCCCACCCCT GCCGGTCACG ATGGCAGCTG GCGTTAGTCA GCCTCTGCTC
    ACCTTGGCTT CATCTGCTGC GGCGGCGGCC GTCCCCGGGG CACCCACTGT GGTCCCTAGT
    CAGCTTCCTG CCCTCCTGCA GCCTGTGACC CAGCTGCCGA GTCAGGCTCA CCCGCAGCTC
    CTGCAGCCAG CAGTTCAGTC CATGGGGATC CCCGCCAGCC TTGGACAGAC TGCCGAGGTG
    CCACTTCCCC CTGGAGATAT TCTCTACCAG GGCTTCCCAC CTCGACTGCC ATCGCAGTAC
    CCAGGAGACT CAAACATTGC CCCCTCCTCC AGCGCAGCTT CTGTGTGCCT GCCTTCTGCA
    GTCCTGTCCC CTCCCATGCC GACAGAGGCG CTGGCTGCCC CGGGTTACTT TCCTGCAATG
    GTGCAGCCCT ATGTGGAATC CAGTCTCTTG GTTCCTGTGG GCAGTGTAGG AGGACAGGTC
    CAGGTGTCCC AGCCGGCAGT GAGCTTAGCA CAAGCCCCCA CCACATCCTC CCAGCAAGCA
    GCTCTGGAGA GCACTCAGGT CGTCTCTCAG GTGGCTCCTC CAGAGCCAGC TCCCGGGGCT
    CAGCCACAGC CTGCCCAGCC AGCCGCGGTG GTCTCTTCTA TAGACAGCGC ACATTCAGAT
    GTGGCTTCAG GCCTGAGTGA TGGCAACGAG AATGTCCCCT CATCCAGCGG GAGGCACGAG
    GGCAGGACCA CGAAGCGGCT GCATCGGAAG TCTATAAGGA GCCGCTCTCG TCACGAGAAG
    CCTTCCCGCC CCAAACTGAG AATTCTCAAT GTTTCAAATA AGGGAGATCG GGTGGTAGAA
    TGTCAGTTAG AGACTCACAA TCGGAAGATG GTTACATTCA AATTTGATTT AGATGGTGAC
    AACCCTGAGG AGATAGCCAC AATTATGGTG AACAATGACT TTATTCTGGC ACTGGAGAGA
    GATGCGTTTG TGGAGCAAGT GCGGGAGATC ATTGAGAAAG CTGACGAGAT GCTCAGTGAG
    GATGTCAGCG TGGAGCCAGA GGGTGACCAG AGCTTGGAGA GTCTCCATGG CAAGGATGAC
    TATGGCTTCT CAGGCACCCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCATCT
    TCCTTGCCAC AACAGATAGC AGGCATTCCT GCCAGTTCTT TAACTCAAGT TGTTCATTCT
    GCTGGAAGAC GGTTTATAGT AAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAACTT
    TTCACTGAAA TTTCAGATAC GGTTGCTGCT TCCACATCTG CTGGCCCTGG AATGAACTTG
    TCTCACTCTG CCTCATCCCT TAGTCTGCAG CAGGCCTTTT CTGAACTTAG ACATGCTCGG
    ATGGCAGAAG GGCCGAGTAC GGCCCCTCCC CACTTTGGTC AGACAGGCCC CGTGTTTCCA
    GCAGTGCCTC CCACCCTGAG CAGTGCCCCT GGAGCCCCAG CCGCAGCAGC CGCAGCCTCG
    GTGTCAGCCC CTGCCCCAAG CCACCCTCTT AGTGACGTGA CCACATCGGT CATTCAGACT
    GAGGTGACAG TGGCCCCTGG AACAGGTGTC ATGCCTCCCA GTGGTCTCCC TGTACCCCCT
    GTGTCTGAGT CCCTGGCACC TTCCAGCGTG GCCCCCAGCG TCACCATCCC TGCAGTTCTC
    TCGGCACTAC CAGCGCCCCA GGCGGCACAG GCTCCCACCC CTGGCAGCGG GGCCTCCAGT
    GCAGGCACTT TCCCCTCTGC ACCCGCATCC CTGACACCAG CCTCTGCTGT GGGCAGCGCT
    GTTGCCTCTG CTGCTCAGCT TCCGCCTGTG CCGTCTCAGC CCGCGGTAGG CAGCTCGACC
    GGGGTGGCCA CGCTGACAGC CGTGCCCGCT GCCGCTCTGG GCCCTAGCCT CACGGCACTG
    CCGTCCCTTG CTCTCAGCAG CAGTACCTCT GCCCCAGCTC TGGCAGAGAC AGTGCTGGTC
    AGTGCCCACG CACTGGATAA GACGGCCCAT AGTAGCACCA CTGGCCTGGC TCTGTCCCTT
    CCTGCCACCT CGTCCTCTCC CGCCCCTGGA GCAGGAGTGT CCAGTGCTGT TTCTCAGCCT
    ACTGCTGCGG CTCACCCTTT GGTCCTCCCA TCAGTCGTAA CCTCTACTCC CGTACTCTCC
    CAGGCAGGAC CCATGTCAGC ACCTCTACTG CCCCAGGTAC CCGGTATTCC ACCCCTGGTA
    CAACCTGTTG TCAGCGTGCC TGCTGTGCAA CAGACCCTGG TTCATAGTCA GCCTCAGCCC
    GCTCCACTCC CCAACCAGCC CCACACTCAC TGTCCCGAGA TAGACGCCGA CACTCAACCC
    AAAGCTCCCG GGATCGACGA CATCAAGACT TTGGAGGAAA AGCTGCGATC TCTCTTTAGT
    GAGCACAGTT CTTCTGGAGC CCAACACGCC CCCGTCTCTC TGGAGACCTC GCTCACTGTA
    GAGACCGCTG TCACAGCAGG CCTCCCAACC ACTGCTGTTG CACCAAGCAA GCCTCTGACT
    TCCACTGCCA GTACTTGCTT GCCACCAACC AGTCTGCCCT TAGGAGCAGC TGGCTTGTCA
    GTTATACCAA TGGTCACTCC TGGTCAAGTT TCCACCCCAG TCAGCTCTGC GCCAGGAGTG
    AAACTTGAAA CTGCTCCAGC AAAGCCACCT TTAACTAAGC CTCCAGTGCT GCCCATGGGC
    ACTGAACTTC CAGCTGGTAC TCCACCCAGC GAGCAGCTGC CACCTTTTCC AGGACCTTCA
    CTAACTCAGC CCCCGCAGCC CCTGGGAGAC CTGGACGCTC AGCTGAGAAG AACCCTCAGT
    CCCGAGAGTG GCATGCTGAC GTCTGCCGTT GGACCCGTGT CTGTGGTGGC TCCAACAGCA
    GTTGTGGAGA CCGAAGCCCA ACTTCCAAAG GATGTTCTTT CAGTCTCTCA AGTCACAGAA
    GCTCCTGTCC TTGCGCCCAC TTCCACAGCT GGTGTTCTGA AGATGGGGCG ATTTCAGGTC
    TCAGTTGCAA TGGATGACCC CCAGAAGGAG GGTAAAAGTA AGTCGGAGGA TGCCAAGTCT
    GTCCACTTCG CATCCAGCAC GTCAGAGTCC TCGGTGCTGT CAAGCAGCAG CCCCGAGAGC
    ACCGTGGTGA AAGCAGAGCC CAACGGGCTG GCCGTCCGGG GCGCCTCTGC AGACATGCCT
    GACAGCGCCC ACCAGCCACC TGCCTCAGAA GCTGGGCAGC CTAGCAAGGT GGGCCGTTTT
    CAGGTGACCA CCACAGCGAG CAGAGTGGGT CGCTTCTCTG TGTCCAGAAC TGAAGACAAG
    ATGGCTGAGG TGAAGGGAGA GGAGCCAGTG GTCTCTCCTT TCATGGACTC AGAACACGAC
    ATTCCTCCCA CCGTGACGCC AAAGAAAGAG AAACCCGAAC TGTCTGAGCC TTCGCATCTG
    AACGGGCCGT CTTCCGACCT GGAGGCTGCC TTCCTCAGCC GGGACGTGGA TGCTGGCTCA
    GGGAGCCCTC ACTCCCCTCC GCAGCTGAGC TCCAAGAGCC TTCCTGCCCA GAGCCTCAGT
    CAGAGCCTCA GCAATTCCTT CAACTCTTCC TACATGAGCA GCGACAATGA GTCAGACATT
    GAAGACGAGG ACTTAAAGTT AGAGCTTCGC CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCACGAA ATTGAGTCTT TATACACCAA ACTGGGCAAG
    GTTCCTCCTG CTGTCATCAT TCCCCCAGCA GCCCCCCTTG CAGGGAGAAG GAGGCGACCC
    ACCAAAAGCA AAGGGAGCAA GTCCAGTCGC AGCAGCTCTT TGGGGAATAA AAGCCCTGGA
    CCAGGTACCC TGTCTGGCCA GAGTGCAGCT TCAGTCTTGC ACCCTCAGCA GACCCTCCCC
    GCTCCTGGCA GCGTCCCGGA GACAGGGCCC AACCAGCTGT TACAGCCTGT GAAGCCATCT
    CCTTCCAGTG ACAACCTGTA CTCGGCCTTT ACCAGCGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCGC CAGGTCAAGG CTGTGCCAAG TTTAACTGTG CGTCCGAGCA GGTGACGTTC
    AAAGCTGGCG GCAGGAGGAC GCGATTTCTG AGTACGCCCT GCTTGGCTCT TTGGAAGATG
    GTGAAAAAAG TCTGCCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCAGA GCCGAAATCA
    GGGACCAGCA GCACCAACGC CGTTGGGGGA GCCGTGAGCA GCCAGGCCGC CCCGGCTCCA
    CCTCCTGCCG TGACGTCCAG CAGGAAGGGC ATGTTCACGG ACGACCTGCA CAAGCTGGTG
    GACAACTGGG CCCGGGACGC CATGAGTCTC TCAGGCAGGC GGGGAAGCAA AGGACACGTG
    AGCTATGAGG GCCCTGGCAT GGCAAGGAAG TTCTCTGCGC CCGGTCAGCT GTGCGTCTCC
    ATGACCTCAA ACCTGGGCGG CTCTGCCCCC GGCTCTGCAG CGTCAGCTAC CTCTCTAGGG
    CACTTCCCCA AGTCCATGTG CCCCCCTCAG CAGTACGGTT TCCCTACCCC CCCGTTTGGC
    ACGCAGTGGA GCGGGACGGG TGGCCCGGCA CCCCAGCCGC TTAGCCAGTT CCAGCCTGTG
    GGAACAGCCT CCTTACAGAA TTTCAACATC AGCAACTTGC AGAAATCCAT CAGCAACCCC
    CCAGGTTCCA ACCTGCGGAC CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207337.1
    CDS88..8634
    Translation

    Target ORF information:

    RefSeq Version XM_005207280.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207280.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCCT
    CAGTCCATGG CGCATCCGCG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC
    CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG
    GCCATTGCCC AGCGGCGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCA CCAGTTCCAA
    CCCCTGCTGA GGACTGTCGG GCAGAGTCTT CTTCCACCTG GTGGCAGCCC ACCTAACTGG
    ACTCCAGAGA CTGTAGTTAT GTGGGGCACT GCAGCCAGTA GAGTTGCTGG AGAGCTGTGT
    GAGGTGCAGG CCCATCCTGT GTTCGAGCCA GCTCCAGTTT ACAGTGACTA CAGAGCTGGA
    CTGATGCTTC CAGAAGAAGC TCACTACTTC CTCCCTCCAG AAGCAGTGTA TCTAGCCGGG
    GTACATTACC AGGCCCAGGT GGCAGAACAG TTTGAGGGTT TTCCATACAG CTCACCAGTC
    CTGTCAAGTC CTATGAAGCA GACACCTGAG CAGAAGCCAG TGCACGGGGG CCCCACCTCC
    AGTTCTGTCT TTGAATTTCC ATCGGGACAG GCTTTCCTGG TAGGACACCT CCAGAATGTA
    AGATTAGATT CGGGACTGAG CCCAGGCTCT CCCCAGTCTA GCGTTTCTGC ACCTATCAGT
    ACAGATGCCA CACGCTTGAA GTTTCACCCT GTCTTTGTTC CTCATTCTGC ACCTGCTGTG
    TTAACTCATA ACAACGAGAG CAGAAGCAGT TGTGTGTTTG AGTTTCACGT TCATACTCCA
    AGCTCCTCTT CAGGAGAAGG AGGGGGAGGA GTTTTACCTC AGCGCATTTA CCGAAATCGG
    CAGGTTGCTG TGGACTCGAA CCAGGAAGAA CCACCTCTTC AACCTGCAGG ACTACACAGC
    CGCCTGCAGC CTGTGACTGA AGAACAGCAG ATGCCCCAGG CCCCAGAGTT GACCATCTCT
    GTGGTGGAGC CCACCGGACA GACCTGGCCC ATAGGAAGCC CAGAGTACTC CAGTGATTCC
    TCCCAGGTCA CTTCTTCAGA CCCCAGTGAA TTTCAGTCAC CTCCCCCTAC AGGGGGAGCA
    GCTGCTCCTT TTGGCTCTGA CGTCTCATTA CCCTTTATCC GCGTGCCTCA GACAGTGTTA
    CAAGAATCCC CACTCTTCTT CTGTTTCCCC CAAGGAGCCA CCTCTCCGCA GGTTTTATCC
    GCCTCATTTT CTTCAGGAGG ATCTGCACTC CATCCACAGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCTT CAAGTAGTTT AACAGGGGTT CCATCCTCCC AACCCATGCA GCATTCTCAG
    CAGCAGCAAG GAGTGCAGCC AACAGCCCCC TCTCAGCAGG CGGTGCAGTA TCCGCTCCCT
    CAGACATCGG CCCCCAGCGA GGCCACGATT GCACAGCCTG TGAGTCAGCC TCCACCTCCA
    CAGGCCTTGC CACACGTGTC CGCGGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT
    CTCCAAGGCG AACCTCAGAT CCCAGTTGCA ACACAGCCCT CAGTCGTTCC CGTCCATGCT
    GGTGCTCACT TCCTTCCTGT GGGACAGCCA CTCCCACCCT CTTTACTGCC CCAGTACCCC
    GTCTCTCAGC TGCCCCCCAT CTCTCAGCTC CCCTCTGCTC CTCCTCACGT GTCTGTGGCT
    CAGCCAGGCT TCCCACCCCT GCCGGTCACG ATGGCAGCTG GCGTTAGTCA GCCTCTGCTC
    ACCTTGGCTT CATCTGCTGC GGCGGCGGCC GTCCCCGGGG CACCCACTGT GGTCCCTAGT
    CAGCTTCCTG CCCTCCTGCA GCCTGTGACC CAGCTGCCGA GTCAGGCTCA CCCGCAGCTC
    CTGCAGCCAG CAGTTCAGTC CATGGGGATC CCCGCCAGCC TTGGACAGAC TGCCGAGGTG
    CCACTTCCCC CTGGAGATAT TCTCTACCAG GGCTTCCCAC CTCGACTGCC ATCGCAGTAC
    CCAGGAGACT CAAACATTGC CCCCTCCTCC AGCGCAGCTT CTGTGTGCCT GCCTTCTGCA
    GTCCTGTCCC CTCCCATGCC GACAGAGGCG CTGGCTGCCC CGGGTTACTT TCCTGCAATG
    GTGCAGCCCT ATGTGGAATC CAGTCTCTTG GTTCCTGTGG GCAGTGTAGG AGGACAGGTC
    CAGGTGTCCC AGCCGGCAGT GAGCTTAGCA CAAGCCCCCA CCACATCCTC CCAGCAAGCA
    GCTCTGGAGA GCACTCAGGT CGTCTCTCAG GTGGCTCCTC CAGAGCCAGC TCCCGGGGCT
    CAGCCACAGC CTGCCCAGCC AGCCGCGGTG GTCTCTTCTA TAGACAGCGC ACATTCAGAT
    GTGGCTTCAG GCCTGAGTGA TGGCAACGAG AATGTCCCCT CATCCAGCGG GAGGCACGAG
    GGCAGGACCA CGAAGCGGCT GCATCGGAAG TCTATAAGGA GCCGCTCTCG TCACGAGAAG
    CCTTCCCGCC CCAAACTGAG AATTCTCAAT GTTTCAAATA AGGGAGATCG GGTGGTAGAA
    TGTCAGTTAG AGACTCACAA TCGGAAGATG GTTACATTCA AATTTGATTT AGATGGTGAC
    AACCCTGAGG AGATAGCCAC AATTATGGTG AACAATGACT TTATTCTGGC ACTGGAGAGA
    GATGCGTTTG TGGAGCAAGT GCGGGAGATC ATTGAGAAAG CTGACGAGAT GCTCAGTGAG
    GATGTCAGCG TGGAGCCAGA GGGTGACCAG AGCTTGGAGA GTCTCCATGG CAAGGATGAC
    TATGGCTTCT CAGGCACCCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCATCT
    TCCTTGCCAC AACAGATAGC AGGCATTCCT GCCAGTTCTT TAACTCAAGT TGTTCATTCT
    GCTGGAAGAC GGTTTATAGT AAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAACTT
    TTCACTGAAA TTTCAGATAC GGTTGCTGCT TCCACATCTG CTGGCCCTGG AATGAACTTG
    TCTCACTCTG CCTCATCCCT TAGTCTGCAG CAGGCCTTTT CTGAACTTAG ACATGCTCGG
    ATGGCAGAAG GGCCGAGTAC GGCCCCTCCC CACTTTGGTC AGACAGGCCC CGTGTTTCCA
    GCAGTGCCTC CCACCCTGAG CAGTGCCCCT GGAGCCCCAG CCGCAGCAGC CGCAGCCTCG
    GTGTCAGCCC CTGCCCCAAG CCACCCTCTT AGTGACGTGA CCACATCGGT CATTCAGACT
    GAGGTGACAG TGGCCCCTGG AACAGGTGTC ATGCCTCCCA GTGGTCTCCC TGTACCCCCT
    GTGTCTGAGT CCCTGGCACC TTCCAGCGTG GCCCCCAGCG TCACCATCCC TGCAGTTCTC
    TCGGCACTAC CAGCGCCCCA GGCGGCACAG GCTCCCACCC CTGGCAGCGG GGCCTCCAGT
    GCAGGCACTT TCCCCTCTGC ACCCGCATCC CTGACACCAG CCTCTGCTGT GGGCAGCGCT
    GTTGCCTCTG CTGCTCAGCT TCCGCCTGTG CCGTCTCAGC CCGCGGTAGG CAGCTCGACC
    GGGGTGGCCA CGCTGACAGC CGTGCCCGCT GCCGCTCTGG GCCCTAGCCT CACGGCACTG
    CCGTCCCTTG CTCTCAGCAG CAGTACCTCT GCCCCAGCTC TGGCAGAGAC AGTGCTGGTC
    AGTGCCCACG CACTGGATAA GACGGCCCAT AGTAGCACCA CTGGCCTGGC TCTGTCCCTT
    CCTGCCACCT CGTCCTCTCC CGCCCCTGGA GCAGGAGTGT CCAGTGCTGT TTCTCAGCCT
    ACTGCTGCGG CTCACCCTTT GGTCCTCCCA TCAGTCGTAA CCTCTACTCC CGTACTCTCC
    CAGGCAGGAC CCATGTCAGC ACCTCTACTG CCCCAGGTAC CCGGTATTCC ACCCCTGGTA
    CAACCTGTTG TCAGCGTGCC TGCTGTGCAA CAGACCCTGG TTCATAGTCA GCCTCAGCCC
    GCTCCACTCC CCAACCAGCC CCACACTCAC TGTCCCGAGA TAGACGCCGA CACTCAACCC
    AAAGCTCCCG GGATCGACGA CATCAAGACT TTGGAGGAAA AGCTGCGATC TCTCTTTAGT
    GAGCACAGTT CTTCTGGAGC CCAACACGCC CCCGTCTCTC TGGAGACCTC GCTCACTGTA
    GAGACCGCTG TCACAGCAGG CCTCCCAACC ACTGCTGTTG CACCAAGCAA GCCTCTGACT
    TCCACTGCCA GTACTTGCTT GCCACCAACC AGTCTGCCCT TAGGAGCAGC TGGCTTGTCA
    GTTATACCAA TGGTCACTCC TGGTCAAGTT TCCACCCCAG TCAGCTCTGC GCCAGGAGTG
    AAACTTGAAA CTGCTCCAGC AAAGCCACCT TTAACTAAGC CTCCAGTGCT GCCCATGGGC
    ACTGAACTTC CAGCTGGTAC TCCACCCAGC GAGCAGCTGC CACCTTTTCC AGGACCTTCA
    CTAACTCAGC CCCCGCAGCC CCTGGGAGAC CTGGACGCTC AGCTGAGAAG AACCCTCAGT
    CCCGAGAGTG GCATGCTGAC GTCTGCCGTT GGACCCGTGT CTGTGGTGGC TCCAACAGCA
    GTTGTGGAGA CCGAAGCCCA ACTTCCAAAG GATGTTCTTT CAGTCTCTCA AGTCACAGAA
    GCTCCTGTCC TTGCGCCCAC TTCCACAGCT GGTGTTCTGA AGATGGGGCG ATTTCAGGTC
    TCAGTTGCAA TGGATGACCC CCAGAAGGAG GGTAAAAGTA AGTCGGAGGA TGCCAAGTCT
    GTCCACTTCG CATCCAGCAC GTCAGAGTCC TCGGTGCTGT CAAGCAGCAG CCCCGAGAGC
    ACCGTGGTGA AAGCAGAGCC CAACGGGCTG GCCGTCCGGG GCGCCTCTGC AGACATGCCT
    GACAGCGCCC ACCAGCCACC TGCCTCAGAA GCTGGGCAGC CTAGCAAGGT GGGCCGTTTT
    CAGGTGACCA CCACAGCGAG CAGAGTGGGT CGCTTCTCTG TGTCCAGAAC TGAAGACAAG
    ATGGCTGAGG TGAAGGGAGA GGAGCCAGTG GTCTCTCCTT TCATGGACTC AGAACACGAC
    ATTCCTCCCA CCGTGACGCC AAAGAAAGAG AAACCCGAAC TGTCTGAGCC TTCGCATCTG
    AACGGGCCGT CTTCCGACCT GGAGGCTGCC TTCCTCAGCC GGGACGTGGA TGCTGGCTCA
    GGGAGCCCTC ACTCCCCTCC GCAGCTGAGC TCCAAGAGCC TTCCTGCCCA GAGCCTCAGT
    CAGAGCCTCA GCAATTCCTT CAACTCTTCC TACATGAGCA GCGACAATGA GTCAGACATT
    GAAGACGAGG ACTTAAAGTT AGAGCTTCGC CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCACGAA ATTGAGTCTT TATACACCAA ACTGGGCAAG
    GTTCCTCCTG CTGTCATCAT TCCCCCAGCA GCCCCCCTTG CAGGGAGAAG GAGGCGACCC
    ACCAAAAGCA AAGGGAGCAA GTCCAGTCGC AGCAGCTCTT TGGGGAATAA AAGCCCTGGA
    CCAGGTACCC TGTCTGGCCA GAGTGCAGCT TCAGTCTTGC ACCCTCAGCA GACCCTCCCC
    GCTCCTGGCA GCGTCCCGGA GACAGGGCCC AACCAGCTGT TACAGCCTGT GAAGCCATCT
    CCTTCCAGTG ACAACCTGTA CTCGGCCTTT ACCAGCGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCGC CAGGTCAAGG CTGTGCCAAG TTTAACTGTG CGTCCGAGCA GGTGACGTTC
    AAAGCTGGCG GCAGGAGGAC GCGATTTCTG AGTACGCCCT GCTTGGCTCT TTGGAAGATG
    GTGAAAAAAG TCTGCCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCAGA GCCGAAATCA
    GGGACCAGCA GCACCAACGC CGTTGGGGGA GCCGTGAGCA GCCAGGCCGC CCCGGCTCCA
    CCTCCTGCCG TGACGTCCAG CAGGAAGGGC ATGTTCACGG ACGACCTGCA CAAGCTGGTG
    GACAACTGGG CCCGGGACGC CATGAGTCTC TCAGGCAGGC GGGGAAGCAA AGGACACGTG
    AGCTATGAGG GCCCTGGCAT GGCAAGGAAG TTCTCTGCGC CCGGTCAGCT GTGCGTCTCC
    ATGACCTCAA ACCTGGGCGG CTCTGCCCCC GGCTCTGCAG CGTCAGCTAC CTCTCTAGGG
    CACTTCCCCA AGTCCATGTG CCCCCCTCAG CAGTACGGTT TCCCTACCCC CCCGTTTGGC
    ACGCAGTGGA GCGGGACGGG TGGCCCGGCA CCCCAGCCGC TTAGCCAGTT CCAGCCTGTG
    GGAACAGCCT CCTTACAGAA TTTCAACATC AGCAACTTGC AGAAATCCAT CAGCAACCCC
    CCAGGTTCCA ACCTGCGGAC CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19898
    Clone ID Related Accession (Same CDS sequence) XM_005207280.3 , XM_015463526.1
    Accession Version XM_015463526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCCT
    CAGTCCATGG CGCATCCGCG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC
    CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG
    GCCATTGCCC AGCGGCGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCA CCAGTTCCAA
    CCCCTGCTGA GGACTGTCGG GCAGAGTCTT CTTCCACCTG GTGGCAGCCC ACCTAACTGG
    ACTCCAGAGA CTGTAGTTAT GTGGGGCACT GCAGCCAGTA GAGTTGCTGG AGAGCTGTGT
    GAGGTGCAGG CCCATCCTGT GTTCGAGCCA GCTCCAGTTT ACAGTGACTA CAGAGCTGGA
    CTGATGCTTC CAGAAGAAGC TCACTACTTC CTCCCTCCAG AAGCAGTGTA TCTAGCCGGG
    GTACATTACC AGGCCCAGGT GGCAGAACAG TTTGAGGGTT TTCCATACAG CTCACCAGTC
    CTGTCAAGTC CTATGAAGCA GACACCTGAG CAGAAGCCAG TGCACGGGGG CCCCACCTCC
    AGTTCTGTCT TTGAATTTCC ATCGGGACAG GCTTTCCTGG TAGGACACCT CCAGAATGTA
    AGATTAGATT CGGGACTGAG CCCAGGCTCT CCCCAGTCTA GCGTTTCTGC ACCTATCAGT
    ACAGATGCCA CACGCTTGAA GTTTCACCCT GTCTTTGTTC CTCATTCTGC ACCTGCTGTG
    TTAACTCATA ACAACGAGAG CAGAAGCAGT TGTGTGTTTG AGTTTCACGT TCATACTCCA
    AGCTCCTCTT CAGGAGAAGG AGGGGGAGGA GTTTTACCTC AGCGCATTTA CCGAAATCGG
    CAGGTTGCTG TGGACTCGAA CCAGGAAGAA CCACCTCTTC AACCTGCAGG ACTACACAGC
    CGCCTGCAGC CTGTGACTGA AGAACAGCAG ATGCCCCAGG CCCCAGAGTT GACCATCTCT
    GTGGTGGAGC CCACCGGACA GACCTGGCCC ATAGGAAGCC CAGAGTACTC CAGTGATTCC
    TCCCAGGTCA CTTCTTCAGA CCCCAGTGAA TTTCAGTCAC CTCCCCCTAC AGGGGGAGCA
    GCTGCTCCTT TTGGCTCTGA CGTCTCATTA CCCTTTATCC GCGTGCCTCA GACAGTGTTA
    CAAGAATCCC CACTCTTCTT CTGTTTCCCC CAAGGAGCCA CCTCTCCGCA GGTTTTATCC
    GCCTCATTTT CTTCAGGAGG ATCTGCACTC CATCCACAGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCTT CAAGTAGTTT AACAGGGGTT CCATCCTCCC AACCCATGCA GCATTCTCAG
    CAGCAGCAAG GAGTGCAGCC AACAGCCCCC TCTCAGCAGG CGGTGCAGTA TCCGCTCCCT
    CAGACATCGG CCCCCAGCGA GGCCACGATT GCACAGCCTG TGAGTCAGCC TCCACCTCCA
    CAGGCCTTGC CACACGTGTC CGCGGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT
    CTCCAAGGCG AACCTCAGAT CCCAGTTGCA ACACAGCCCT CAGTCGTTCC CGTCCATGCT
    GGTGCTCACT TCCTTCCTGT GGGACAGCCA CTCCCACCCT CTTTACTGCC CCAGTACCCC
    GTCTCTCAGC TGCCCCCCAT CTCTCAGCTC CCCTCTGCTC CTCCTCACGT GTCTGTGGCT
    CAGCCAGGCT TCCCACCCCT GCCGGTCACG ATGGCAGCTG GCGTTAGTCA GCCTCTGCTC
    ACCTTGGCTT CATCTGCTGC GGCGGCGGCC GTCCCCGGGG CACCCACTGT GGTCCCTAGT
    CAGCTTCCTG CCCTCCTGCA GCCTGTGACC CAGCTGCCGA GTCAGGCTCA CCCGCAGCTC
    CTGCAGCCAG CAGTTCAGTC CATGGGGATC CCCGCCAGCC TTGGACAGAC TGCCGAGGTG
    CCACTTCCCC CTGGAGATAT TCTCTACCAG GGCTTCCCAC CTCGACTGCC ATCGCAGTAC
    CCAGGAGACT CAAACATTGC CCCCTCCTCC AGCGCAGCTT CTGTGTGCCT GCCTTCTGCA
    GTCCTGTCCC CTCCCATGCC GACAGAGGCG CTGGCTGCCC CGGGTTACTT TCCTGCAATG
    GTGCAGCCCT ATGTGGAATC CAGTCTCTTG GTTCCTGTGG GCAGTGTAGG AGGACAGGTC
    CAGGTGTCCC AGCCGGCAGT GAGCTTAGCA CAAGCCCCCA CCACATCCTC CCAGCAAGCA
    GCTCTGGAGA GCACTCAGGT CGTCTCTCAG GTGGCTCCTC CAGAGCCAGC TCCCGGGGCT
    CAGCCACAGC CTGCCCAGCC AGCCGCGGTG GTCTCTTCTA TAGACAGCGC ACATTCAGAT
    GTGGCTTCAG GCCTGAGTGA TGGCAACGAG AATGTCCCCT CATCCAGCGG GAGGCACGAG
    GGCAGGACCA CGAAGCGGCT GCATCGGAAG TCTATAAGGA GCCGCTCTCG TCACGAGAAG
    CCTTCCCGCC CCAAACTGAG AATTCTCAAT GTTTCAAATA AGGGAGATCG GGTGGTAGAA
    TGTCAGTTAG AGACTCACAA TCGGAAGATG GTTACATTCA AATTTGATTT AGATGGTGAC
    AACCCTGAGG AGATAGCCAC AATTATGGTG AACAATGACT TTATTCTGGC ACTGGAGAGA
    GATGCGTTTG TGGAGCAAGT GCGGGAGATC ATTGAGAAAG CTGACGAGAT GCTCAGTGAG
    GATGTCAGCG TGGAGCCAGA GGGTGACCAG AGCTTGGAGA GTCTCCATGG CAAGGATGAC
    TATGGCTTCT CAGGCACCCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCATCT
    TCCTTGCCAC AACAGATAGC AGGCATTCCT GCCAGTTCTT TAACTCAAGT TGTTCATTCT
    GCTGGAAGAC GGTTTATAGT AAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAACTT
    TTCACTGAAA TTTCAGATAC GGTTGCTGCT TCCACATCTG CTGGCCCTGG AATGAACTTG
    TCTCACTCTG CCTCATCCCT TAGTCTGCAG CAGGCCTTTT CTGAACTTAG ACATGCTCGG
    ATGGCAGAAG GGCCGAGTAC GGCCCCTCCC CACTTTGGTC AGACAGGCCC CGTGTTTCCA
    GCAGTGCCTC CCACCCTGAG CAGTGCCCCT GGAGCCCCAG CCGCAGCAGC CGCAGCCTCG
    GTGTCAGCCC CTGCCCCAAG CCACCCTCTT AGTGACGTGA CCACATCGGT CATTCAGACT
    GAGGTGACAG TGGCCCCTGG AACAGGTGTC ATGCCTCCCA GTGGTCTCCC TGTACCCCCT
    GTGTCTGAGT CCCTGGCACC TTCCAGCGTG GCCCCCAGCG TCACCATCCC TGCAGTTCTC
    TCGGCACTAC CAGCGCCCCA GGCGGCACAG GCTCCCACCC CTGGCAGCGG GGCCTCCAGT
    GCAGGCACTT TCCCCTCTGC ACCCGCATCC CTGACACCAG CCTCTGCTGT GGGCAGCGCT
    GTTGCCTCTG CTGCTCAGCT TCCGCCTGTG CCGTCTCAGC CCGCGGTAGG CAGCTCGACC
    GGGGTGGCCA CGCTGACAGC CGTGCCCGCT GCCGCTCTGG GCCCTAGCCT CACGGCACTG
    CCGTCCCTTG CTCTCAGCAG CAGTACCTCT GCCCCAGCTC TGGCAGAGAC AGTGCTGGTC
    AGTGCCCACG CACTGGATAA GACGGCCCAT AGTAGCACCA CTGGCCTGGC TCTGTCCCTT
    CCTGCCACCT CGTCCTCTCC CGCCCCTGGA GCAGGAGTGT CCAGTGCTGT TTCTCAGCCT
    ACTGCTGCGG CTCACCCTTT GGTCCTCCCA TCAGTCGTAA CCTCTACTCC CGTACTCTCC
    CAGGCAGGAC CCATGTCAGC ACCTCTACTG CCCCAGGTAC CCGGTATTCC ACCCCTGGTA
    CAACCTGTTG TCAGCGTGCC TGCTGTGCAA CAGACCCTGG TTCATAGTCA GCCTCAGCCC
    GCTCCACTCC CCAACCAGCC CCACACTCAC TGTCCCGAGA TAGACGCCGA CACTCAACCC
    AAAGCTCCCG GGATCGACGA CATCAAGACT TTGGAGGAAA AGCTGCGATC TCTCTTTAGT
    GAGCACAGTT CTTCTGGAGC CCAACACGCC CCCGTCTCTC TGGAGACCTC GCTCACTGTA
    GAGACCGCTG TCACAGCAGG CCTCCCAACC ACTGCTGTTG CACCAAGCAA GCCTCTGACT
    TCCACTGCCA GTACTTGCTT GCCACCAACC AGTCTGCCCT TAGGAGCAGC TGGCTTGTCA
    GTTATACCAA TGGTCACTCC TGGTCAAGTT TCCACCCCAG TCAGCTCTGC GCCAGGAGTG
    AAACTTGAAA CTGCTCCAGC AAAGCCACCT TTAACTAAGC CTCCAGTGCT GCCCATGGGC
    ACTGAACTTC CAGCTGGTAC TCCACCCAGC GAGCAGCTGC CACCTTTTCC AGGACCTTCA
    CTAACTCAGC CCCCGCAGCC CCTGGGAGAC CTGGACGCTC AGCTGAGAAG AACCCTCAGT
    CCCGAGAGTG GCATGCTGAC GTCTGCCGTT GGACCCGTGT CTGTGGTGGC TCCAACAGCA
    GTTGTGGAGA CCGAAGCCCA ACTTCCAAAG GATGTTCTTT CAGTCTCTCA AGTCACAGAA
    GCTCCTGTCC TTGCGCCCAC TTCCACAGCT GGTGTTCTGA AGATGGGGCG ATTTCAGGTC
    TCAGTTGCAA TGGATGACCC CCAGAAGGAG GGTAAAAGTA AGTCGGAGGA TGCCAAGTCT
    GTCCACTTCG CATCCAGCAC GTCAGAGTCC TCGGTGCTGT CAAGCAGCAG CCCCGAGAGC
    ACCGTGGTGA AAGCAGAGCC CAACGGGCTG GCCGTCCGGG GCGCCTCTGC AGACATGCCT
    GACAGCGCCC ACCAGCCACC TGCCTCAGAA GCTGGGCAGC CTAGCAAGGT GGGCCGTTTT
    CAGGTGACCA CCACAGCGAG CAGAGTGGGT CGCTTCTCTG TGTCCAGAAC TGAAGACAAG
    ATGGCTGAGG TGAAGGGAGA GGAGCCAGTG GTCTCTCCTT TCATGGACTC AGAACACGAC
    ATTCCTCCCA CCGTGACGCC AAAGAAAGAG AAACCCGAAC TGTCTGAGCC TTCGCATCTG
    AACGGGCCGT CTTCCGACCT GGAGGCTGCC TTCCTCAGCC GGGACGTGGA TGCTGGCTCA
    GGGAGCCCTC ACTCCCCTCC GCAGCTGAGC TCCAAGAGCC TTCCTGCCCA GAGCCTCAGT
    CAGAGCCTCA GCAATTCCTT CAACTCTTCC TACATGAGCA GCGACAATGA GTCAGACATT
    GAAGACGAGG ACTTAAAGTT AGAGCTTCGC CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCACGAA ATTGAGTCTT TATACACCAA ACTGGGCAAG
    GTTCCTCCTG CTGTCATCAT TCCCCCAGCA GCCCCCCTTG CAGGGAGAAG GAGGCGACCC
    ACCAAAAGCA AAGGGAGCAA GTCCAGTCGC AGCAGCTCTT TGGGGAATAA AAGCCCTGGA
    CCAGGTACCC TGTCTGGCCA GAGTGCAGCT TCAGTCTTGC ACCCTCAGCA GACCCTCCCC
    GCTCCTGGCA GCGTCCCGGA GACAGGGCCC AACCAGCTGT TACAGCCTGT GAAGCCATCT
    CCTTCCAGTG ACAACCTGTA CTCGGCCTTT ACCAGCGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCGC CAGGTCAAGG CTGTGCCAAG TTTAACTGTG CGTCCGAGCA GGTGACGTTC
    AAAGCTGGCG GCAGGAGGAC GCGATTTCTG AGTACGCCCT GCTTGGCTCT TTGGAAGATG
    GTGAAAAAAG TCTGCCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCAGA GCCGAAATCA
    GGGACCAGCA GCACCAACGC CGTTGGGGGA GCCGTGAGCA GCCAGGCCGC CCCGGCTCCA
    CCTCCTGCCG TGACGTCCAG CAGGAAGGGC ATGTTCACGG ACGACCTGCA CAAGCTGGTG
    GACAACTGGG CCCGGGACGC CATGAGTCTC TCAGGCAGGC GGGGAAGCAA AGGACACGTG
    AGCTATGAGG GCCCTGGCAT GGCAAGGAAG TTCTCTGCGC CCGGTCAGCT GTGCGTCTCC
    ATGACCTCAA ACCTGGGCGG CTCTGCCCCC GGCTCTGCAG CGTCAGCTAC CTCTCTAGGG
    CACTTCCCCA AGTCCATGTG CCCCCCTCAG CAGTACGGTT TCCCTACCCC CCCGTTTGGC
    ACGCAGTGGA GCGGGACGGG TGGCCCGGCA CCCCAGCCGC TTAGCCAGTT CCAGCCTGTG
    GGAACAGCCT CCTTACAGAA TTTCAACATC AGCAACTTGC AGAAATCCAT CAGCAACCCC
    CCAGGTTCCA ACCTGCGGAC CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319012.1
    CDS88..8634
    Translation

    Target ORF information:

    RefSeq Version XM_015463526.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGTCTGGCG GCGCCGCCGA CCAGCAGAGC AGCGCGCAGG GCTCGCTGTT CCTCTCGCCC 
    CCGGCGCCTG CCCCCAAGAA TGGCTCCAGC TCGGACTCCT CGGTGGGGGA GAAACTGGGC
    GCCGCAGCGG CCGACGCCGC AGCCGGCCGG ACGGAGGAGT ACCGGCGTCG CCGCCATACC
    ATGGACAAGG ACAGCCGGGG GGCGGCCGCG ACCACCACCA CAGAGCACCG CTTCTTCCGC
    CGCAGCGTCA TCTGCGACTC CAACGCTACG GCCCTGGAGC TGCCCGGCCT CCCTCTGCCG
    CTGCCTCAGC CCGGCGCGGC TGCCGTGGTC CCGCAGCGGA GCCCCCCGGA ACCCCCGCGC
    GAGGAGACCC TGATCCCCGC CGCTGCCGCC CAGGTGGCCC AGCAGCCGCC CGCCGCCGCC
    GCCGCCGCCG CCGCCGCCGC CCCTGAGGAG CCGGTCGCCG CGAGCCCGGC CACCTCTTCG
    GCCCCGAGCA GCGCGGGCAG AGACCGCCAG GCTGTCCAGC CGGGCCCCGC GGGGAGCCGA
    GAGGAGCCTC CGACGTCGAG AAGTGGCAGC GGCGGCGGCA GCGCCAAGGA GCCCCAGGAG
    GAACGGAGCC AGCAGCAGGA TGACATCGAG GAGTTGGAGA CCAAGGCCGT GGGCATGTCC
    AACGACGGCC GCTTTCTCAA GTTTGACATC GAGATTGGCA GGGGCTCCTT TAAGACGGTC
    TACAAGGGTT TGGACACCGA GACCACCGTG GAGGTCGCCT GGTGTGAGCT GCAGGATCGA
    AAGTTAACAA AGTCTGAGAG GCAGCGATTT AAAGAAGAGG CTGAAATGTT AAAGGGTCTT
    CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAAT CCACAGTAAA GGGAAAGAAG
    TGCATTGTGT TGGTGACTGA ACTGATGACA TCTGGAACTC TTAAAACGTA CCTGAAAAGG
    TTTAAGGTGA TGAAGATCAA AGTTTTGAGA AGCTGGTGCC GTCAGATTCT TAAAGGACTT
    CAGTTTCTTC ATACTAGAAC TCCACCTATT ATTCACCGGG ATCTTAAGTG TGACAACATC
    TTTATCACTG GCCCCACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC CACCCTGAAG
    CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT
    GAAGAGAAGT ACGATGAATC CGTTGATGTT TATGCTTTTG GGATGTGCAT GCTCGAGATG
    GCCACGTCTG AATACCCGTA CTCAGAGTGT CAGAACGCTG CACAGATCTA CCGTCGTGTG
    ACCAGCGGGG TGAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT
    ATTGAAGGAT GCATACGGCA AAACAAAGAT GAAAGATACT CTATCAAAGA CCTTTTGAAC
    CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA
    GAAAAGATAG CCATTAAATT ATGGCTACGT ATTGAAGATA TTAAGAAACT AAAGGGGAAA
    TACAAAGACA ATGAAGCTAT CGAGTTTTCC TTTGACTTGG AGAAAGATGT GCCAGAAGAC
    GTTGCTCAAG AAATGGTAGA TTCTGGGTAT GTCTGTGAAG GTGATCACAA GACCATGGCA
    AAAGCCATCA AAGATAGAGT GTCGTTAATT AAGAGAAAAC GAGAACAGCG TCAATTAGTG
    CGGGAGGAGC AAGAAAAAAG AAAGCAGGAG GAGAGCAGCT TCAAGCAGCA GGTGGAGCAG
    CAGCCCAGCA CTGCCCAGGC CGGAGTCCAG CAGCCCCCTG CCACCAGCAC TGGTGGACCC
    GCTGCCTCTG CTGCGTCTGC GTCCGTCTCC ACGCAGGTAG AGCCAGAAGA GCCTGAGGCC
    GACCAGCACC AGCAGCTGCA GTACCAGCAG CCTGGTGTGT CTGTGATATC TGACGGGACG
    GTTGACAGTG GTCAGGGGTC TTCTGTCTTC ACGGAATCTC GAGTCAGCAG CCAACAGACA
    GTTTCGTACG GGTCCCAGCA TGAGCAGGCA CCTTCCACTG GCGCCCTCCC AGGGCACACA
    GCATCTGTTG TCCAAACGCA GGCTCAGCCC CATGCGGTAT ATCCACCCTC AAGTTTGCCT
    CAGTCCATGG CGCATCCGCG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC
    CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG
    GCCATTGCCC AGCGGCGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCA CCAGTTCCAA
    CCCCTGCTGA GGACTGTCGG GCAGAGTCTT CTTCCACCTG GTGGCAGCCC ACCTAACTGG
    ACTCCAGAGA CTGTAGTTAT GTGGGGCACT GCAGCCAGTA GAGTTGCTGG AGAGCTGTGT
    GAGGTGCAGG CCCATCCTGT GTTCGAGCCA GCTCCAGTTT ACAGTGACTA CAGAGCTGGA
    CTGATGCTTC CAGAAGAAGC TCACTACTTC CTCCCTCCAG AAGCAGTGTA TCTAGCCGGG
    GTACATTACC AGGCCCAGGT GGCAGAACAG TTTGAGGGTT TTCCATACAG CTCACCAGTC
    CTGTCAAGTC CTATGAAGCA GACACCTGAG CAGAAGCCAG TGCACGGGGG CCCCACCTCC
    AGTTCTGTCT TTGAATTTCC ATCGGGACAG GCTTTCCTGG TAGGACACCT CCAGAATGTA
    AGATTAGATT CGGGACTGAG CCCAGGCTCT CCCCAGTCTA GCGTTTCTGC ACCTATCAGT
    ACAGATGCCA CACGCTTGAA GTTTCACCCT GTCTTTGTTC CTCATTCTGC ACCTGCTGTG
    TTAACTCATA ACAACGAGAG CAGAAGCAGT TGTGTGTTTG AGTTTCACGT TCATACTCCA
    AGCTCCTCTT CAGGAGAAGG AGGGGGAGGA GTTTTACCTC AGCGCATTTA CCGAAATCGG
    CAGGTTGCTG TGGACTCGAA CCAGGAAGAA CCACCTCTTC AACCTGCAGG ACTACACAGC
    CGCCTGCAGC CTGTGACTGA AGAACAGCAG ATGCCCCAGG CCCCAGAGTT GACCATCTCT
    GTGGTGGAGC CCACCGGACA GACCTGGCCC ATAGGAAGCC CAGAGTACTC CAGTGATTCC
    TCCCAGGTCA CTTCTTCAGA CCCCAGTGAA TTTCAGTCAC CTCCCCCTAC AGGGGGAGCA
    GCTGCTCCTT TTGGCTCTGA CGTCTCATTA CCCTTTATCC GCGTGCCTCA GACAGTGTTA
    CAAGAATCCC CACTCTTCTT CTGTTTCCCC CAAGGAGCCA CCTCTCCGCA GGTTTTATCC
    GCCTCATTTT CTTCAGGAGG ATCTGCACTC CATCCACAGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCTT CAAGTAGTTT AACAGGGGTT CCATCCTCCC AACCCATGCA GCATTCTCAG
    CAGCAGCAAG GAGTGCAGCC AACAGCCCCC TCTCAGCAGG CGGTGCAGTA TCCGCTCCCT
    CAGACATCGG CCCCCAGCGA GGCCACGATT GCACAGCCTG TGAGTCAGCC TCCACCTCCA
    CAGGCCTTGC CACACGTGTC CGCGGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT
    CTCCAAGGCG AACCTCAGAT CCCAGTTGCA ACACAGCCCT CAGTCGTTCC CGTCCATGCT
    GGTGCTCACT TCCTTCCTGT GGGACAGCCA CTCCCACCCT CTTTACTGCC CCAGTACCCC
    GTCTCTCAGC TGCCCCCCAT CTCTCAGCTC CCCTCTGCTC CTCCTCACGT GTCTGTGGCT
    CAGCCAGGCT TCCCACCCCT GCCGGTCACG ATGGCAGCTG GCGTTAGTCA GCCTCTGCTC
    ACCTTGGCTT CATCTGCTGC GGCGGCGGCC GTCCCCGGGG CACCCACTGT GGTCCCTAGT
    CAGCTTCCTG CCCTCCTGCA GCCTGTGACC CAGCTGCCGA GTCAGGCTCA CCCGCAGCTC
    CTGCAGCCAG CAGTTCAGTC CATGGGGATC CCCGCCAGCC TTGGACAGAC TGCCGAGGTG
    CCACTTCCCC CTGGAGATAT TCTCTACCAG GGCTTCCCAC CTCGACTGCC ATCGCAGTAC
    CCAGGAGACT CAAACATTGC CCCCTCCTCC AGCGCAGCTT CTGTGTGCCT GCCTTCTGCA
    GTCCTGTCCC CTCCCATGCC GACAGAGGCG CTGGCTGCCC CGGGTTACTT TCCTGCAATG
    GTGCAGCCCT ATGTGGAATC CAGTCTCTTG GTTCCTGTGG GCAGTGTAGG AGGACAGGTC
    CAGGTGTCCC AGCCGGCAGT GAGCTTAGCA CAAGCCCCCA CCACATCCTC CCAGCAAGCA
    GCTCTGGAGA GCACTCAGGT CGTCTCTCAG GTGGCTCCTC CAGAGCCAGC TCCCGGGGCT
    CAGCCACAGC CTGCCCAGCC AGCCGCGGTG GTCTCTTCTA TAGACAGCGC ACATTCAGAT
    GTGGCTTCAG GCCTGAGTGA TGGCAACGAG AATGTCCCCT CATCCAGCGG GAGGCACGAG
    GGCAGGACCA CGAAGCGGCT GCATCGGAAG TCTATAAGGA GCCGCTCTCG TCACGAGAAG
    CCTTCCCGCC CCAAACTGAG AATTCTCAAT GTTTCAAATA AGGGAGATCG GGTGGTAGAA
    TGTCAGTTAG AGACTCACAA TCGGAAGATG GTTACATTCA AATTTGATTT AGATGGTGAC
    AACCCTGAGG AGATAGCCAC AATTATGGTG AACAATGACT TTATTCTGGC ACTGGAGAGA
    GATGCGTTTG TGGAGCAAGT GCGGGAGATC ATTGAGAAAG CTGACGAGAT GCTCAGTGAG
    GATGTCAGCG TGGAGCCAGA GGGTGACCAG AGCTTGGAGA GTCTCCATGG CAAGGATGAC
    TATGGCTTCT CAGGCACCCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCATCT
    TCCTTGCCAC AACAGATAGC AGGCATTCCT GCCAGTTCTT TAACTCAAGT TGTTCATTCT
    GCTGGAAGAC GGTTTATAGT AAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAACTT
    TTCACTGAAA TTTCAGATAC GGTTGCTGCT TCCACATCTG CTGGCCCTGG AATGAACTTG
    TCTCACTCTG CCTCATCCCT TAGTCTGCAG CAGGCCTTTT CTGAACTTAG ACATGCTCGG
    ATGGCAGAAG GGCCGAGTAC GGCCCCTCCC CACTTTGGTC AGACAGGCCC CGTGTTTCCA
    GCAGTGCCTC CCACCCTGAG CAGTGCCCCT GGAGCCCCAG CCGCAGCAGC CGCAGCCTCG
    GTGTCAGCCC CTGCCCCAAG CCACCCTCTT AGTGACGTGA CCACATCGGT CATTCAGACT
    GAGGTGACAG TGGCCCCTGG AACAGGTGTC ATGCCTCCCA GTGGTCTCCC TGTACCCCCT
    GTGTCTGAGT CCCTGGCACC TTCCAGCGTG GCCCCCAGCG TCACCATCCC TGCAGTTCTC
    TCGGCACTAC CAGCGCCCCA GGCGGCACAG GCTCCCACCC CTGGCAGCGG GGCCTCCAGT
    GCAGGCACTT TCCCCTCTGC ACCCGCATCC CTGACACCAG CCTCTGCTGT GGGCAGCGCT
    GTTGCCTCTG CTGCTCAGCT TCCGCCTGTG CCGTCTCAGC CCGCGGTAGG CAGCTCGACC
    GGGGTGGCCA CGCTGACAGC CGTGCCCGCT GCCGCTCTGG GCCCTAGCCT CACGGCACTG
    CCGTCCCTTG CTCTCAGCAG CAGTACCTCT GCCCCAGCTC TGGCAGAGAC AGTGCTGGTC
    AGTGCCCACG CACTGGATAA GACGGCCCAT AGTAGCACCA CTGGCCTGGC TCTGTCCCTT
    CCTGCCACCT CGTCCTCTCC CGCCCCTGGA GCAGGAGTGT CCAGTGCTGT TTCTCAGCCT
    ACTGCTGCGG CTCACCCTTT GGTCCTCCCA TCAGTCGTAA CCTCTACTCC CGTACTCTCC
    CAGGCAGGAC CCATGTCAGC ACCTCTACTG CCCCAGGTAC CCGGTATTCC ACCCCTGGTA
    CAACCTGTTG TCAGCGTGCC TGCTGTGCAA CAGACCCTGG TTCATAGTCA GCCTCAGCCC
    GCTCCACTCC CCAACCAGCC CCACACTCAC TGTCCCGAGA TAGACGCCGA CACTCAACCC
    AAAGCTCCCG GGATCGACGA CATCAAGACT TTGGAGGAAA AGCTGCGATC TCTCTTTAGT
    GAGCACAGTT CTTCTGGAGC CCAACACGCC CCCGTCTCTC TGGAGACCTC GCTCACTGTA
    GAGACCGCTG TCACAGCAGG CCTCCCAACC ACTGCTGTTG CACCAAGCAA GCCTCTGACT
    TCCACTGCCA GTACTTGCTT GCCACCAACC AGTCTGCCCT TAGGAGCAGC TGGCTTGTCA
    GTTATACCAA TGGTCACTCC TGGTCAAGTT TCCACCCCAG TCAGCTCTGC GCCAGGAGTG
    AAACTTGAAA CTGCTCCAGC AAAGCCACCT TTAACTAAGC CTCCAGTGCT GCCCATGGGC
    ACTGAACTTC CAGCTGGTAC TCCACCCAGC GAGCAGCTGC CACCTTTTCC AGGACCTTCA
    CTAACTCAGC CCCCGCAGCC CCTGGGAGAC CTGGACGCTC AGCTGAGAAG AACCCTCAGT
    CCCGAGAGTG GCATGCTGAC GTCTGCCGTT GGACCCGTGT CTGTGGTGGC TCCAACAGCA
    GTTGTGGAGA CCGAAGCCCA ACTTCCAAAG GATGTTCTTT CAGTCTCTCA AGTCACAGAA
    GCTCCTGTCC TTGCGCCCAC TTCCACAGCT GGTGTTCTGA AGATGGGGCG ATTTCAGGTC
    TCAGTTGCAA TGGATGACCC CCAGAAGGAG GGTAAAAGTA AGTCGGAGGA TGCCAAGTCT
    GTCCACTTCG CATCCAGCAC GTCAGAGTCC TCGGTGCTGT CAAGCAGCAG CCCCGAGAGC
    ACCGTGGTGA AAGCAGAGCC CAACGGGCTG GCCGTCCGGG GCGCCTCTGC AGACATGCCT
    GACAGCGCCC ACCAGCCACC TGCCTCAGAA GCTGGGCAGC CTAGCAAGGT GGGCCGTTTT
    CAGGTGACCA CCACAGCGAG CAGAGTGGGT CGCTTCTCTG TGTCCAGAAC TGAAGACAAG
    ATGGCTGAGG TGAAGGGAGA GGAGCCAGTG GTCTCTCCTT TCATGGACTC AGAACACGAC
    ATTCCTCCCA CCGTGACGCC AAAGAAAGAG AAACCCGAAC TGTCTGAGCC TTCGCATCTG
    AACGGGCCGT CTTCCGACCT GGAGGCTGCC TTCCTCAGCC GGGACGTGGA TGCTGGCTCA
    GGGAGCCCTC ACTCCCCTCC GCAGCTGAGC TCCAAGAGCC TTCCTGCCCA GAGCCTCAGT
    CAGAGCCTCA GCAATTCCTT CAACTCTTCC TACATGAGCA GCGACAATGA GTCAGACATT
    GAAGACGAGG ACTTAAAGTT AGAGCTTCGC CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCACGAA ATTGAGTCTT TATACACCAA ACTGGGCAAG
    GTTCCTCCTG CTGTCATCAT TCCCCCAGCA GCCCCCCTTG CAGGGAGAAG GAGGCGACCC
    ACCAAAAGCA AAGGGAGCAA GTCCAGTCGC AGCAGCTCTT TGGGGAATAA AAGCCCTGGA
    CCAGGTACCC TGTCTGGCCA GAGTGCAGCT TCAGTCTTGC ACCCTCAGCA GACCCTCCCC
    GCTCCTGGCA GCGTCCCGGA GACAGGGCCC AACCAGCTGT TACAGCCTGT GAAGCCATCT
    CCTTCCAGTG ACAACCTGTA CTCGGCCTTT ACCAGCGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCGC CAGGTCAAGG CTGTGCCAAG TTTAACTGTG CGTCCGAGCA GGTGACGTTC
    AAAGCTGGCG GCAGGAGGAC GCGATTTCTG AGTACGCCCT GCTTGGCTCT TTGGAAGATG
    GTGAAAAAAG TCTGCCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCAGA GCCGAAATCA
    GGGACCAGCA GCACCAACGC CGTTGGGGGA GCCGTGAGCA GCCAGGCCGC CCCGGCTCCA
    CCTCCTGCCG TGACGTCCAG CAGGAAGGGC ATGTTCACGG ACGACCTGCA CAAGCTGGTG
    GACAACTGGG CCCGGGACGC CATGAGTCTC TCAGGCAGGC GGGGAAGCAA AGGACACGTG
    AGCTATGAGG GCCCTGGCAT GGCAAGGAAG TTCTCTGCGC CCGGTCAGCT GTGCGTCTCC
    ATGACCTCAA ACCTGGGCGG CTCTGCCCCC GGCTCTGCAG CGTCAGCTAC CTCTCTAGGG
    CACTTCCCCA AGTCCATGTG CCCCCCTCAG CAGTACGGTT TCCCTACCCC CCCGTTTGGC
    ACGCAGTGGA GCGGGACGGG TGGCCCGGCA CCCCAGCCGC TTAGCCAGTT CCAGCCTGTG
    GGAACAGCCT CCTTACAGAA TTTCAACATC AGCAACTTGC AGAAATCCAT CAGCAACCCC
    CCAGGTTCCA ACCTGCGGAC CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.