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NECAB2 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol NECAB2
Entrez Gene ID 515049
Full Name N-terminal EF-hand calcium binding protein 2
General protein information
Preferred Names

N-terminal EF-hand calcium binding protein 2

Names

N-terminal EF-hand calcium-binding protein 2
neuronal calcium-binding protein 2
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_010814355.2 XP_010812657.1 N-terminal EF-hand calcium-binding protein 2 isoform X1
XM_010814356.1 XP_010812658.1 N-terminal EF-hand calcium-binding protein 2 isoform X2
XM_010814357.2 XP_010812659.1 N-terminal EF-hand calcium-binding protein 2 isoform X2
XM_010814360.2 XP_010812662.2 N-terminal EF-hand calcium-binding protein 2 isoform X1
XM_010814356.2 XP_010812658.1 N-terminal EF-hand calcium-binding protein 2 isoform X4
XM_015475686.1 XP_015331172.1 N-terminal EF-hand calcium-binding protein 2 isoform X2
XM_024978207.1 XP_024833975.1 N-terminal EF-hand calcium-binding protein 2 isoform X2
XM_010814357.3 XP_010812659.1 N-terminal EF-hand calcium-binding protein 2 isoform X4
NM_001098977.1 NP_001092447.1 N-terminal EF-hand calcium-binding protein 2
XM_024978208.1 XP_024833976.1 N-terminal EF-hand calcium-binding protein 2 isoform X4
XM_010814355.3 XP_010812657.1 N-terminal EF-hand calcium-binding protein 2 isoform X3
XM_010814360.1 XP_010812662.1 N-terminal EF-hand calcium-binding protein 2 isoform X3

Pathways from BioSystems


Homologies


Homo sapiens (human) NECAB2 NP_061938.2
Bos taurus (cattle) NECAB2 NP_001092447.1
Danio rerio (zebrafish) necab2 NP_001025366.1
Gallus gallus (chicken) NECAB2 XP_414072.1
Xenopus tropicalis (tropical clawed frog) LOC101731915 XP_004913648.1
Pan troglodytes (chimpanzee) NECAB2 XP_001150555.1
LOC718169 XP_001110489.2
Mus musculus (house mouse) Necab2 NP_473436.1
Rattus norvegicus (Norway rat) Necab2 NP_596906.1
Canis lupus familiaris (dog) NECAB2 XP_005620942.1

Gene Ontology


Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NECAB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NECAB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa36032 NM_001098977.1
Latest version!		 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OBa36032 XM_010814360.1
Latest version!		 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OBa49175 XM_010814355.3
Latest version!		 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OBa49176 XM_010814357.3
Latest version!		 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OBa49176 XM_024978208.1
Latest version!		 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OBa49176 XM_015475686.1
Latest version!		 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OBa49176 XM_010814356.2
Latest version!		 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OBa224983 XM_010814360.2
Latest version!		 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OBa224984 XM_024978207.1
Latest version!		 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OBa49176 XM_010814356.1 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OBa49176 XM_010814357.2 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OBa49175 XM_010814355.2 Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa36032
Clone ID Related Accession (Same CDS sequence) NM_001098977.1 , XM_010814360.1
Accession Version NM_001098977.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 783bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-13
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC146196.1. On Jun 26, 2007 this sequence version replaced XM_592995.3. ##Evidence-Data-START## Transcript exon combination :: BC146196.1, DV869309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145419 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGACT ATGAGGACGT CCTGGCTTCC CTGGAAACCT TGAACCACTC CGTGCTGAAG 
GCCATGGGCT ACACCAAGAA GGTGTACGAG GGCGGGAGCA ACGTGGACCA GTTTGTGACG 
CGCTTCCTCC TGAAGGAGAC GGCCAATCAG ATCCAGTCCC TGCTGAGCTC AGTGGAGAGT 
GCGGTGGAGG CCATCGAAGA GCAGACCAGC CAGATCCGAC AGAACCACAG CCGGCCCAGC 
CATGGCGCGG TGGAGACCTG GTCCGGGAGT CCCACGCCCC CCTACGCCCC CAACCACAAG 
CTCATGGCTG CGGAGCAAGG AAAGAGCTTC CCACCTGGCA CCTCGGACCC CAAGGAGGAC 
GGCCTGGACG CCCAGATCAG CCGGCTGGCG GGGCTCATTG GACGGCTGGA GAACAAGACC 
CTTTGGTTTG ACCTTCAGCA GCATCTCTCG GACGAAGAAG GCACCAACAT GCACCTGCAG 
CTGGTCCGGC AGGAGATGGC CGTGTGCCCC GAGCAGCTGG GTGACTTCCT GGACTCTCTG 
CGCCAGTATG TGCGGGGCAC CGCTGGGACC AGGAACTGCT TCCACATCGC TGCCGTGAGG 
CTGGCGGACG GCGTCACCTT CGTGGTCTAT GAGTTCTGGG AGACGGAGGA GGAGTGGAAG 
CGGCACCTAC AGAGCCCTGT GTGCAAGGCA TTCCGGCACG TCAAGGTGGA CACGCTGAGC 
CAGCCTGAGG CCTTCTCCCG GATCTCCGTG CCAGCTGCTT GGTGCACCCT GGGCCGTGAC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001092447.1
CDS114..896
Translation

Target ORF information:

RefSeq Version NM_001098977.1
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001098977.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGACT ATGAGGACGT CCTGGCTTCC CTGGAAACCT TGAACCACTC CGTGCTGAAG 
GCCATGGGCT ACACCAAGAA GGTGTACGAG GGCGGGAGCA ACGTGGACCA GTTTGTGACG 
CGCTTCCTCC TGAAGGAGAC GGCCAATCAG ATCCAGTCCC TGCTGAGCTC AGTGGAGAGT 
GCGGTGGAGG CCATCGAAGA GCAGACCAGC CAGATCCGAC AGAACCACAG CCGGCCCAGC 
CATGGCGCGG TGGAGACCTG GTCCGGGAGT CCCACGCCCC CCTACGCCCC CAACCACAAG 
CTCATGGCTG CGGAGCAAGG AAAGAGCTTC CCACCTGGCA CCTCGGACCC CAAGGAGGAC 
GGCCTGGACG CCCAGATCAG CCGGCTGGCG GGGCTCATTG GACGGCTGGA GAACAAGACC 
CTTTGGTTTG ACCTTCAGCA GCATCTCTCG GACGAAGAAG GCACCAACAT GCACCTGCAG 
CTGGTCCGGC AGGAGATGGC CGTGTGCCCC GAGCAGCTGG GTGACTTCCT GGACTCTCTG 
CGCCAGTATG TGCGGGGCAC CGCTGGGACC AGGAACTGCT TCCACATCGC TGCCGTGAGG 
CTGGCGGACG GCGTCACCTT CGTGGTCTAT GAGTTCTGGG AGACGGAGGA GGAGTGGAAG 
CGGCACCTAC AGAGCCCTGT GTGCAAGGCA TTCCGGCACG TCAAGGTGGA CACGCTGAGC 
CAGCCTGAGG CCTTCTCCCG GATCTCCGTG CCAGCTGCTT GGTGCACCCT GGGCCGTGAC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa36032
Clone ID Related Accession (Same CDS sequence) NM_001098977.1 , XM_010814360.1
Accession Version XM_010814360.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 783bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGACT ATGAGGACGT CCTGGCTTCC CTGGAAACCT TGAACCACTC CGTGCTGAAG 
GCCATGGGCT ACACCAAGAA GGTGTACGAG GGCGGGAGCA ACGTGGACCA GTTTGTGACG 
CGCTTCCTCC TGAAGGAGAC GGCCAATCAG ATCCAGTCCC TGCTGAGCTC AGTGGAGAGT 
GCGGTGGAGG CCATCGAAGA GCAGACCAGC CAGATCCGAC AGAACCACAG CCGGCCCAGC 
CATGGCGCGG TGGAGACCTG GTCCGGGAGT CCCACGCCCC CCTACGCCCC CAACCACAAG 
CTCATGGCTG CGGAGCAAGG AAAGAGCTTC CCACCTGGCA CCTCGGACCC CAAGGAGGAC 
GGCCTGGACG CCCAGATCAG CCGGCTGGCG GGGCTCATTG GACGGCTGGA GAACAAGACC 
CTTTGGTTTG ACCTTCAGCA GCATCTCTCG GACGAAGAAG GCACCAACAT GCACCTGCAG 
CTGGTCCGGC AGGAGATGGC CGTGTGCCCC GAGCAGCTGG GTGACTTCCT GGACTCTCTG 
CGCCAGTATG TGCGGGGCAC CGCTGGGACC AGGAACTGCT TCCACATCGC TGCCGTGAGG 
CTGGCGGACG GCGTCACCTT CGTGGTCTAT GAGTTCTGGG AGACGGAGGA GGAGTGGAAG 
CGGCACCTAC AGAGCCCTGT GTGCAAGGCA TTCCGGCACG TCAAGGTGGA CACGCTGAGC 
CAGCCTGAGG CCTTCTCCCG GATCTCCGTG CCAGCTGCTT GGTGCACCCT GGGCCGTGAC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010812662.1
CDS190..972
Translation

Target ORF information:

RefSeq Version XM_010814360.1
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010814360.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGACT ATGAGGACGT CCTGGCTTCC CTGGAAACCT TGAACCACTC CGTGCTGAAG 
GCCATGGGCT ACACCAAGAA GGTGTACGAG GGCGGGAGCA ACGTGGACCA GTTTGTGACG 
CGCTTCCTCC TGAAGGAGAC GGCCAATCAG ATCCAGTCCC TGCTGAGCTC AGTGGAGAGT 
GCGGTGGAGG CCATCGAAGA GCAGACCAGC CAGATCCGAC AGAACCACAG CCGGCCCAGC 
CATGGCGCGG TGGAGACCTG GTCCGGGAGT CCCACGCCCC CCTACGCCCC CAACCACAAG 
CTCATGGCTG CGGAGCAAGG AAAGAGCTTC CCACCTGGCA CCTCGGACCC CAAGGAGGAC 
GGCCTGGACG CCCAGATCAG CCGGCTGGCG GGGCTCATTG GACGGCTGGA GAACAAGACC 
CTTTGGTTTG ACCTTCAGCA GCATCTCTCG GACGAAGAAG GCACCAACAT GCACCTGCAG 
CTGGTCCGGC AGGAGATGGC CGTGTGCCCC GAGCAGCTGG GTGACTTCCT GGACTCTCTG 
CGCCAGTATG TGCGGGGCAC CGCTGGGACC AGGAACTGCT TCCACATCGC TGCCGTGAGG 
CTGGCGGACG GCGTCACCTT CGTGGTCTAT GAGTTCTGGG AGACGGAGGA GGAGTGGAAG 
CGGCACCTAC AGAGCCCTGT GTGCAAGGCA TTCCGGCACG TCAAGGTGGA CACGCTGAGC 
CAGCCTGAGG CCTTCTCCCG GATCTCCGTG CCAGCTGCTT GGTGCACCCT GGGCCGTGAC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa49175
Clone ID Related Accession (Same CDS sequence) XM_010814355.2 , XM_010814355.3
Accession Version XM_010814355.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCCTCTCC GTGGCCTTAA GATTTTCCGC CGTGCGGACA AAAACGATGA CGGGAAGTTG 
TCCTTGGAGG AGTTCCAGCT CTTCTTTGCA GATGGCGTCC TCAACGAGAG GGAACTGGAG 
GATCTCTTTC ACACCATCGA CTCTGACAAC ACCAACCATG TGGACACCAA GGAGCTTTGT 
GACTACTTTG TGGACCACAT GGGGGACTAT GAGGACGTCC TGGCTTCCCT GGAAACCTTG 
AACCACTCCG TGCTGAAGGC CATGGGCTAC ACCAAGAAGG TGTACGAGGG CGGGAGCAAC 
GTGGACCAGT TTGTGACGCG CTTCCTCCTG AAGGAGACGG CCAATCAGAT CCAGTCCCTG 
CTGAGCTCAG TGGAGAGTGC GGTGGAGGCC ATCGAAGAGC AGACCAGCCA GATCCGACAG 
AACCACAGCC GGCCCAGCCA TGGCGCGGTG GAGACCTGGT CCGGGAGTCC CACGCCCCCC 
TACGCCCCCA ACCACAAGCT CATGGCTGCG GAGCAAGGAA AGAGCTTCCC ACCTGGCACC 
TCGGACCCCA AGGAGGACGG CCTGGACGCC CAGATCAGCC GGCTGGCGGG GCTCATTGGA 
CGGCTGGAGA ACAAGACCCT TTGGTTTGAC CTTCAGCAGC ATCTCTCGGA CGAAGAAGGC 
ACCAACATGC ACCTGCAGCT GGTCCGGCAG GAGATGGCCG TGTGCCCCGA GCAGCTGGGT 
GACTTCCTGG ACTCTCTGCG CCAGTATGTG CGGGGCACCG CTGGGACCAG GAACTGCTTC 
CACATCGCTG CCGTGAGGCT GGCGGACGGC GTCACCTTCG TGGTCTATGA GTTCTGGGAG 
ACGGAGGAGG AGTGGAAGCG GCACCTACAG AGCCCTGTGT GCAAGGCATT CCGGCACGTC 
AAGGTGGACA CGCTGAGCCA GCCTGAGGCC TTCTCCCGGA TCTCCGTGCC AGCTGCTTGG 
TGCACCCTGG GCCGTGACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010812657.1
CDS248..1228
Translation

Target ORF information:

RefSeq Version XM_010814355.2
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010814355.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCCTCTCC GTGGCCTTAA GATTTTCCGC CGTGCGGACA AAAACGATGA CGGGAAGTTG 
TCCTTGGAGG AGTTCCAGCT CTTCTTTGCA GATGGCGTCC TCAACGAGAG GGAACTGGAG 
GATCTCTTTC ACACCATCGA CTCTGACAAC ACCAACCATG TGGACACCAA GGAGCTTTGT 
GACTACTTTG TGGACCACAT GGGGGACTAT GAGGACGTCC TGGCTTCCCT GGAAACCTTG 
AACCACTCCG TGCTGAAGGC CATGGGCTAC ACCAAGAAGG TGTACGAGGG CGGGAGCAAC 
GTGGACCAGT TTGTGACGCG CTTCCTCCTG AAGGAGACGG CCAATCAGAT CCAGTCCCTG 
CTGAGCTCAG TGGAGAGTGC GGTGGAGGCC ATCGAAGAGC AGACCAGCCA GATCCGACAG 
AACCACAGCC GGCCCAGCCA TGGCGCGGTG GAGACCTGGT CCGGGAGTCC CACGCCCCCC 
TACGCCCCCA ACCACAAGCT CATGGCTGCG GAGCAAGGAA AGAGCTTCCC ACCTGGCACC 
TCGGACCCCA AGGAGGACGG CCTGGACGCC CAGATCAGCC GGCTGGCGGG GCTCATTGGA 
CGGCTGGAGA ACAAGACCCT TTGGTTTGAC CTTCAGCAGC ATCTCTCGGA CGAAGAAGGC 
ACCAACATGC ACCTGCAGCT GGTCCGGCAG GAGATGGCCG TGTGCCCCGA GCAGCTGGGT 
GACTTCCTGG ACTCTCTGCG CCAGTATGTG CGGGGCACCG CTGGGACCAG GAACTGCTTC 
CACATCGCTG CCGTGAGGCT GGCGGACGGC GTCACCTTCG TGGTCTATGA GTTCTGGGAG 
ACGGAGGAGG AGTGGAAGCG GCACCTACAG AGCCCTGTGT GCAAGGCATT CCGGCACGTC 
AAGGTGGACA CGCTGAGCCA GCCTGAGGCC TTCTCCCGGA TCTCCGTGCC AGCTGCTTGG 
TGCACCCTGG GCCGTGACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa49175
Clone ID Related Accession (Same CDS sequence) XM_010814355.2 , XM_010814355.3
Accession Version XM_010814355.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCCTCTCC GTGGCCTTAA GATTTTCCGC CGTGCGGACA AAAACGATGA CGGGAAGTTG 
TCCTTGGAGG AGTTCCAGCT CTTCTTTGCA GATGGCGTCC TCAACGAGAG GGAACTGGAG 
GATCTCTTTC ACACCATCGA CTCTGACAAC ACCAACCATG TGGACACCAA GGAGCTTTGT 
GACTACTTTG TGGACCACAT GGGGGACTAT GAGGACGTCC TGGCTTCCCT GGAAACCTTG 
AACCACTCCG TGCTGAAGGC CATGGGCTAC ACCAAGAAGG TGTACGAGGG CGGGAGCAAC 
GTGGACCAGT TTGTGACGCG CTTCCTCCTG AAGGAGACGG CCAATCAGAT CCAGTCCCTG 
CTGAGCTCAG TGGAGAGTGC GGTGGAGGCC ATCGAAGAGC AGACCAGCCA GATCCGACAG 
AACCACAGCC GGCCCAGCCA TGGCGCGGTG GAGACCTGGT CCGGGAGTCC CACGCCCCCC 
TACGCCCCCA ACCACAAGCT CATGGCTGCG GAGCAAGGAA AGAGCTTCCC ACCTGGCACC 
TCGGACCCCA AGGAGGACGG CCTGGACGCC CAGATCAGCC GGCTGGCGGG GCTCATTGGA 
CGGCTGGAGA ACAAGACCCT TTGGTTTGAC CTTCAGCAGC ATCTCTCGGA CGAAGAAGGC 
ACCAACATGC ACCTGCAGCT GGTCCGGCAG GAGATGGCCG TGTGCCCCGA GCAGCTGGGT 
GACTTCCTGG ACTCTCTGCG CCAGTATGTG CGGGGCACCG CTGGGACCAG GAACTGCTTC 
CACATCGCTG CCGTGAGGCT GGCGGACGGC GTCACCTTCG TGGTCTATGA GTTCTGGGAG 
ACGGAGGAGG AGTGGAAGCG GCACCTACAG AGCCCTGTGT GCAAGGCATT CCGGCACGTC 
AAGGTGGACA CGCTGAGCCA GCCTGAGGCC TTCTCCCGGA TCTCCGTGCC AGCTGCTTGG 
TGCACCCTGG GCCGTGACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010812657.1
CDS61..1041
Translation

Target ORF information:

RefSeq Version XM_010814355.3
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010814355.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCCTCTCC GTGGCCTTAA GATTTTCCGC CGTGCGGACA AAAACGATGA CGGGAAGTTG 
TCCTTGGAGG AGTTCCAGCT CTTCTTTGCA GATGGCGTCC TCAACGAGAG GGAACTGGAG 
GATCTCTTTC ACACCATCGA CTCTGACAAC ACCAACCATG TGGACACCAA GGAGCTTTGT 
GACTACTTTG TGGACCACAT GGGGGACTAT GAGGACGTCC TGGCTTCCCT GGAAACCTTG 
AACCACTCCG TGCTGAAGGC CATGGGCTAC ACCAAGAAGG TGTACGAGGG CGGGAGCAAC 
GTGGACCAGT TTGTGACGCG CTTCCTCCTG AAGGAGACGG CCAATCAGAT CCAGTCCCTG 
CTGAGCTCAG TGGAGAGTGC GGTGGAGGCC ATCGAAGAGC AGACCAGCCA GATCCGACAG 
AACCACAGCC GGCCCAGCCA TGGCGCGGTG GAGACCTGGT CCGGGAGTCC CACGCCCCCC 
TACGCCCCCA ACCACAAGCT CATGGCTGCG GAGCAAGGAA AGAGCTTCCC ACCTGGCACC 
TCGGACCCCA AGGAGGACGG CCTGGACGCC CAGATCAGCC GGCTGGCGGG GCTCATTGGA 
CGGCTGGAGA ACAAGACCCT TTGGTTTGAC CTTCAGCAGC ATCTCTCGGA CGAAGAAGGC 
ACCAACATGC ACCTGCAGCT GGTCCGGCAG GAGATGGCCG TGTGCCCCGA GCAGCTGGGT 
GACTTCCTGG ACTCTCTGCG CCAGTATGTG CGGGGCACCG CTGGGACCAG GAACTGCTTC 
CACATCGCTG CCGTGAGGCT GGCGGACGGC GTCACCTTCG TGGTCTATGA GTTCTGGGAG 
ACGGAGGAGG AGTGGAAGCG GCACCTACAG AGCCCTGTGT GCAAGGCATT CCGGCACGTC 
AAGGTGGACA CGCTGAGCCA GCCTGAGGCC TTCTCCCGGA TCTCCGTGCC AGCTGCTTGG 
TGCACCCTGG GCCGTGACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa49176
Clone ID Related Accession (Same CDS sequence) XM_010814356.1 , XM_010814357.2 , XM_010814356.2 , XM_015475686.1 , XM_010814357.3 , XM_024978208.1
Accession Version XM_010814356.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 909bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010812658.1
CDS38..946
Translation

Target ORF information:

RefSeq Version XM_010814356.1
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010814356.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa49176
Clone ID Related Accession (Same CDS sequence) XM_010814356.1 , XM_010814357.2 , XM_010814356.2 , XM_015475686.1 , XM_010814357.3 , XM_024978208.1
Accession Version XM_010814357.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 909bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010812659.1
CDS362..1270
Translation

Target ORF information:

RefSeq Version XM_010814357.2
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010814357.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa49176
Clone ID Related Accession (Same CDS sequence) XM_010814356.1 , XM_010814357.2 , XM_010814356.2 , XM_015475686.1 , XM_010814357.3 , XM_024978208.1
Accession Version XM_010814356.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 909bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010812658.1
CDS99..1007
Translation

Target ORF information:

RefSeq Version XM_010814356.2
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010814356.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa49176
Clone ID Related Accession (Same CDS sequence) XM_010814356.1 , XM_010814357.2 , XM_010814356.2 , XM_015475686.1 , XM_010814357.3 , XM_024978208.1
Accession Version XM_015475686.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 909bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015331172.1
CDS113..1021
Translation

Target ORF information:

RefSeq Version XM_015475686.1
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475686.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa49176
Clone ID Related Accession (Same CDS sequence) XM_010814356.1 , XM_010814357.2 , XM_010814356.2 , XM_015475686.1 , XM_010814357.3 , XM_024978208.1
Accession Version XM_010814357.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 909bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814357.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010812659.1
CDS343..1251
Translation

Target ORF information:

RefSeq Version XM_010814357.3
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010814357.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa49176
Clone ID Related Accession (Same CDS sequence) XM_010814356.1 , XM_010814357.2 , XM_010814356.2 , XM_015475686.1 , XM_010814357.3 , XM_024978208.1
Accession Version XM_024978208.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 909bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024833976.1
CDS100..1008
Translation

Target ORF information:

RefSeq Version XM_024978208.1
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024978208.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACGGGAA GTTGTCCTTG GAGGAGTTCC AGCTCTTCTT TGCAGATGGC GTCCTCAACG 
AGAGGGAACT GGAGGATCTC TTTCACACCA TCGACTCTGA CAACACCAAA CTACTTTGTG 
GACCACATGG GGGACTATGA GGACGTCCTG GCTTCCCTGG AAACCTTGAA CCACTCCGTG 
CTGAAGGCCA TGGGCTACAC CAAGAAGGTG TACGAGGGCG GGAGCAACGT GGACCAGTTT 
GTGACGCGCT TCCTCCTGAA GGAGACGGCC AATCAGATCC AGTCCCTGCT GAGCTCAGTG 
GAGAGTGCGG TGGAGGCCAT CGAAGAGCAG ACCAGCCAGA TCCGACAGAA CCACAGCCGG 
CCCAGCCATG GCGCGGTGGA GACCTGGTCC GGGAGTCCCA CGCCCCCCTA CGCCCCCAAC 
CACAAGCTCA TGGCTGCGGA GCAAGGAAAG AGCTTCCCAC CTGGCACCTC GGACCCCAAG 
GAGGACGGCC TGGACGCCCA GATCAGCCGG CTGGCGGGGC TCATTGGACG GCTGGAGAAC 
AAGACCCTTT GGTTTGACCT TCAGCAGCAT CTCTCGGACG AAGAAGGCAC CAACATGCAC 
CTGCAGCTGG TCCGGCAGGA GATGGCCGTG TGCCCCGAGC AGCTGGGTGA CTTCCTGGAC 
TCTCTGCGCC AGTATGTGCG GGGCACCGCT GGGACCAGGA ACTGCTTCCA CATCGCTGCC 
GTGAGGCTGG CGGACGGCGT CACCTTCGTG GTCTATGAGT TCTGGGAGAC GGAGGAGGAG 
TGGAAGCGGC ACCTACAGAG CCCTGTGTGC AAGGCATTCC GGCACGTCAA GGTGGACACG 
CTGAGCCAGC CTGAGGCCTT CTCCCGGATC TCCGTGCCAG CTGCTTGGTG CACCCTGGGC 
CGTGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa224983
Clone ID Related Accession (Same CDS sequence) XM_010814360.2
Accession Version XM_010814360.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTGCGAGC GGGCGGCGCG CCTGTGCAGG GCCGGCGCGC ACAGGCTGCT CCGGGAGCCG 
CCGCCGCAGG GCCGGGCGCT GGGCGGGCTG CTGCGCTGGG TGGGCGCCAG GATGGGCGAG 
CCCCGGGCGC CGCTGGCCCC CGACGCCCCC GACACCCCCG ACACCCCCGT CGCGGACCCC 
GGCCCCTACC CGGACCCCGC CTCGCCGCGC GGGGGCACCG CCGTCATCCT GGACATTTTC 
CGCCGTGCGG ACAAAAACGA TGACGGGAAG TTGTCCTTGG AGGAGTTCCA GCTCTTCTTT 
GCAGATGGCG TCCTCAACGA GAGGGAACTG GAGGATCTCT TTCACACCAT CGACTCTGAC 
AACACCAACC ATGTGGACAC CAAGGAGCTT TGTGACTACT TTGTGGACCA CATGGGGGAC 
TATGAGGACG TCCTGGCTTC CCTGGAAACC TTGAACCACT CCGTGCTGAA GGCCATGGGC 
TACACCAAGA AGGTGTACGA GGGCGGGAGC AACGTGGACC AGTTTGTGAC GCGCTTCCTC 
CTGAAGGAGA CGGCCAATCA GATCCAGTCC CTGCTGAGCT CAGTGGAGAG TGCGGTGGAG 
GCCATCGAAG AGCAGACCAG CCAGATCCGA CAGAACCACA GCCGGCCCAG CCATGGCGCG 
GTGGAGACCT GGTCCGGGAG TCCCACGCCC CCCTACGCCC CCAACCACAA GCTCATGGCT 
GCGGAGCAAG GAAAGAGCTT CCCACCTGGC ACCTCGGACC CCAAGGAGGA CGGCCTGGAC 
GCCCAGATCA GCCGGCTGGC GGGGCTCATT GGACGGCTGG AGAACAAGAC CCTTTGGTTT 
GACCTTCAGC AGCATCTCTC GGACGAAGAA GGCACCAACA TGCACCTGCA GCTGGTCCGG 
CAGGAGATGG CCGTGTGCCC CGAGCAGCTG GGTGACTTCC TGGACTCTCT GCGCCAGTAT 
GTGCGGGGCA CCGCTGGGAC CAGGAACTGC TTCCACATCG CTGCCGTGAG GCTGGCGGAC 
GGCGTCACCT TCGTGGTCTA TGAGTTCTGG GAGACGGAGG AGGAGTGGAA GCGGCACCTA 
CAGAGCCCTG TGTGCAAGGC ATTCCGGCAC GTCAAGGTGG ACACGCTGAG CCAGCCTGAG 
GCCTTCTCCC GGATCTCCGT GCCAGCTGCT TGGTGCACCC TGGGCCGTGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010812662.2
CDS1..1194
Translation

Target ORF information:

RefSeq Version XM_010814360.2
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010814360.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTGCGAGC GGGCGGCGCG CCTGTGCAGG GCCGGCGCGC ACAGGCTGCT CCGGGAGCCG 
CCGCCGCAGG GCCGGGCGCT GGGCGGGCTG CTGCGCTGGG TGGGCGCCAG GATGGGCGAG 
CCCCGGGCGC CGCTGGCCCC CGACGCCCCC GACACCCCCG ACACCCCCGT CGCGGACCCC 
GGCCCCTACC CGGACCCCGC CTCGCCGCGC GGGGGCACCG CCGTCATCCT GGACATTTTC 
CGCCGTGCGG ACAAAAACGA TGACGGGAAG TTGTCCTTGG AGGAGTTCCA GCTCTTCTTT 
GCAGATGGCG TCCTCAACGA GAGGGAACTG GAGGATCTCT TTCACACCAT CGACTCTGAC 
AACACCAACC ATGTGGACAC CAAGGAGCTT TGTGACTACT TTGTGGACCA CATGGGGGAC 
TATGAGGACG TCCTGGCTTC CCTGGAAACC TTGAACCACT CCGTGCTGAA GGCCATGGGC 
TACACCAAGA AGGTGTACGA GGGCGGGAGC AACGTGGACC AGTTTGTGAC GCGCTTCCTC 
CTGAAGGAGA CGGCCAATCA GATCCAGTCC CTGCTGAGCT CAGTGGAGAG TGCGGTGGAG 
GCCATCGAAG AGCAGACCAG CCAGATCCGA CAGAACCACA GCCGGCCCAG CCATGGCGCG 
GTGGAGACCT GGTCCGGGAG TCCCACGCCC CCCTACGCCC CCAACCACAA GCTCATGGCT 
GCGGAGCAAG GAAAGAGCTT CCCACCTGGC ACCTCGGACC CCAAGGAGGA CGGCCTGGAC 
GCCCAGATCA GCCGGCTGGC GGGGCTCATT GGACGGCTGG AGAACAAGAC CCTTTGGTTT 
GACCTTCAGC AGCATCTCTC GGACGAAGAA GGCACCAACA TGCACCTGCA GCTGGTCCGG 
CAGGAGATGG CCGTGTGCCC CGAGCAGCTG GGTGACTTCC TGGACTCTCT GCGCCAGTAT 
GTGCGGGGCA CCGCTGGGAC CAGGAACTGC TTCCACATCG CTGCCGTGAG GCTGGCGGAC 
GGCGTCACCT TCGTGGTCTA TGAGTTCTGG GAGACGGAGG AGGAGTGGAA GCGGCACCTA 
CAGAGCCCTG TGTGCAAGGC ATTCCGGCAC GTCAAGGTGG ACACGCTGAG CCAGCCTGAG 
GCCTTCTCCC GGATCTCCGT GCCAGCTGCT TGGTGCACCC TGGGCCGTGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa224984
Clone ID Related Accession (Same CDS sequence) XM_024978207.1
Accession Version XM_024978207.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1026bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product N-terminal EF-hand calcium-binding protein 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCCCAC GCAACAGGCC TCTCAGATGC AGAGTGGGAA ACCCAGACTG CCCGGACTCA 
GACCAGGGTG GAAACAGTAG CCACAGGACA CGATTTTCCG CCGTGCGGAC AAAAACGATG 
ACGGGAAGTT GTCCTTGGAG GAGTTCCAGC TCTTCTTTGC AGATGGCGTC CTCAACGAGA 
GGGAACTGGA GGATCTCTTT CACACCATCG ACTCTGACAA CACCAAACTA CTTTGTGGAC 
CACATGGGGG ACTATGAGGA CGTCCTGGCT TCCCTGGAAA CCTTGAACCA CTCCGTGCTG 
AAGGCCATGG GCTACACCAA GAAGGTGTAC GAGGGCGGGA GCAACGTGGA CCAGTTTGTG 
ACGCGCTTCC TCCTGAAGGA GACGGCCAAT CAGATCCAGT CCCTGCTGAG CTCAGTGGAG 
AGTGCGGTGG AGGCCATCGA AGAGCAGACC AGCCAGATCC GACAGAACCA CAGCCGGCCC 
AGCCATGGCG CGGTGGAGAC CTGGTCCGGG AGTCCCACGC CCCCCTACGC CCCCAACCAC 
AAGCTCATGG CTGCGGAGCA AGGAAAGAGC TTCCCACCTG GCACCTCGGA CCCCAAGGAG 
GACGGCCTGG ACGCCCAGAT CAGCCGGCTG GCGGGGCTCA TTGGACGGCT GGAGAACAAG 
ACCCTTTGGT TTGACCTTCA GCAGCATCTC TCGGACGAAG AAGGCACCAA CATGCACCTG 
CAGCTGGTCC GGCAGGAGAT GGCCGTGTGC CCCGAGCAGC TGGGTGACTT CCTGGACTCT 
CTGCGCCAGT ATGTGCGGGG CACCGCTGGG ACCAGGAACT GCTTCCACAT CGCTGCCGTG 
AGGCTGGCGG ACGGCGTCAC CTTCGTGGTC TATGAGTTCT GGGAGACGGA GGAGGAGTGG 
AAGCGGCACC TACAGAGCCC TGTGTGCAAG GCATTCCGGC ACGTCAAGGT GGACACGCTG 
AGCCAGCCTG AGGCCTTCTC CCGGATCTCC GTGCCAGCTG CTTGGTGCAC CCTGGGCCGT 
GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024833975.1
CDS34..1059
Translation

Target ORF information:

RefSeq Version XM_024978207.1
Organism Bos taurus(cattle)
Definition Bos taurus N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024978207.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCCCAC GCAACAGGCC TCTCAGATGC AGAGTGGGAA ACCCAGACTG CCCGGACTCA 
GACCAGGGTG GAAACAGTAG CCACAGGACA CGATTTTCCG CCGTGCGGAC AAAAACGATG 
ACGGGAAGTT GTCCTTGGAG GAGTTCCAGC TCTTCTTTGC AGATGGCGTC CTCAACGAGA 
GGGAACTGGA GGATCTCTTT CACACCATCG ACTCTGACAA CACCAAACTA CTTTGTGGAC 
CACATGGGGG ACTATGAGGA CGTCCTGGCT TCCCTGGAAA CCTTGAACCA CTCCGTGCTG 
AAGGCCATGG GCTACACCAA GAAGGTGTAC GAGGGCGGGA GCAACGTGGA CCAGTTTGTG 
ACGCGCTTCC TCCTGAAGGA GACGGCCAAT CAGATCCAGT CCCTGCTGAG CTCAGTGGAG 
AGTGCGGTGG AGGCCATCGA AGAGCAGACC AGCCAGATCC GACAGAACCA CAGCCGGCCC 
AGCCATGGCG CGGTGGAGAC CTGGTCCGGG AGTCCCACGC CCCCCTACGC CCCCAACCAC 
AAGCTCATGG CTGCGGAGCA AGGAAAGAGC TTCCCACCTG GCACCTCGGA CCCCAAGGAG 
GACGGCCTGG ACGCCCAGAT CAGCCGGCTG GCGGGGCTCA TTGGACGGCT GGAGAACAAG 
ACCCTTTGGT TTGACCTTCA GCAGCATCTC TCGGACGAAG AAGGCACCAA CATGCACCTG 
CAGCTGGTCC GGCAGGAGAT GGCCGTGTGC CCCGAGCAGC TGGGTGACTT CCTGGACTCT 
CTGCGCCAGT ATGTGCGGGG CACCGCTGGG ACCAGGAACT GCTTCCACAT CGCTGCCGTG 
AGGCTGGCGG ACGGCGTCAC CTTCGTGGTC TATGAGTTCT GGGAGACGGA GGAGGAGTGG 
AAGCGGCACC TACAGAGCCC TGTGTGCAAG GCATTCCGGC ACGTCAAGGT GGACACGCTG 
AGCCAGCCTG AGGCCTTCTC CCGGATCTCC GTGCCAGCTG CTTGGTGCAC CCTGGGCCGT 
GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL