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EEF2K cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol EEF2K
Entrez Gene ID 521730
Full Name eukaryotic elongation factor 2 kinase
General protein information
Preferred Names

eukaryotic elongation factor 2 kinase

Names

eukaryotic elongation factor 2 kinase
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_010819305.1 XP_010817607.1 eukaryotic elongation factor 2 kinase isoform X3
NM_001192542.1 NP_001179471.1 eukaryotic elongation factor 2 kinase
XM_005224739.3 XP_005224796.1 eukaryotic elongation factor 2 kinase isoform X1
XM_005224740.3 XP_005224797.1 eukaryotic elongation factor 2 kinase isoform X2
XM_005224740.4 XP_005224797.1 eukaryotic elongation factor 2 kinase isoform X2
XM_010819305.2 XP_010817607.1 eukaryotic elongation factor 2 kinase isoform X3
XM_005224739.4 XP_005224796.1 eukaryotic elongation factor 2 kinase isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) EEF2K NP_037434.1
Bos taurus (cattle) EEF2K NP_001179471.1
EEF2K XP_001092565.1
Canis lupus familiaris (dog) EEF2K XP_005621491.1
Pan troglodytes (chimpanzee) EEF2K XP_001161085.2
Rattus norvegicus (Norway rat) Eef2k NP_037079.1
Danio rerio (zebrafish) eef2k NP_001002740.1
Caenorhabditis elegans (roundworm) efk-1 NP_001022572.1
Xenopus tropicalis (tropical clawed frog) eef2k NP_001120146.1
Mus musculus (house mouse) Eef2k NP_001254640.1
Gallus gallus (chicken) EEF2K XP_004945358.1

Gene Ontology


Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EEF2K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa50453 XM_010819305.2
Latest version!		 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OBa32973 NM_001192542.1
Latest version!		 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OBa32973 XM_005224739.4
Latest version!		 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OBa32974 XM_005224740.4
Latest version!		 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OBa32974 XM_005224740.3 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OBa50453 XM_010819305.1 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OBa32973 XM_005224739.3 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa50453
Clone ID Related Accession (Same CDS sequence) XM_010819305.1 , XM_010819305.2
Accession Version XM_010819305.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product eukaryotic elongation factor 2 kinase isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCTGACC CCTGGGCTGA GTTCCACCTG GAAGACGTTG CCACGGAATG TGCCACACGC 
CACAGGTACA ACGCTGTTAC CGGCGAGTGG GTGGAGGATG AAGTCCTAAT CAAAATGGCA 
TCCCAGCCCT TCGGCCGGGG AGCAATGAGG GAGTGCTTCA GAACGAAGAA GCTCTCCAAC 
TTCCTGCACA CCCAGCAGTG GAAGGGGGCC TCCAACTACG TGGCAAAGCG CTACATCGAG 
TCCGTGGACA GGGACGTGTA CTTCGAGGAC GTGCGTCTAC AGATGGAGGC CAAGCTCTGG 
GGAGAGGAGT ATAATCGGCA CAAGCCCCCC AAGCAGGTGG ACATCATGCA GATGTGCATC 
ATAGAGCTGA AGGAGAGACC GGGCCGGCCG CTATTCCACC TGGAACACTA CATCGAGGGC 
AAGTACATCA AATATAACTC CAACTCAGGC TTCGTCCGTG ATGACAACGT GCGCCTGACG 
CCACAGGCCT TCAGCCACTT CACGTTTGAG CGTTCCGGCC ACCAGCTGAT TGTGGTGGAC 
ATCCAGGGCG TGGGTGACCT CTATACCGAC CCACAGATCC ACACTGAGAG GGGCACTGAC 
TTTGGAGATG GAAACCTAGG TGTCCGCGGG ATGGCTCTCT TCTTCCACTC ACATGCCTGC 
AACCGGATTT GCAAGAGCAT GGGCCTCACT CCCTTTGACC TGTCCCCGAG AGAGAGGGAT 
GCAGTGAATC AGAACACCAA GCTGCTGCAG TCAGCCAAGA CCATCTTAAG GGGGACAGAG 
GAAAAGTGCG GGAGCCCCCG GGTGAGGACC CTCTCAGGGA GCCGGCCCCC ACTGCTGCTT 
CGCCTCTCAG AGAACTCCGG AGATGACAAC ATGAGCGACG TGACCTTTGA CTCTCTCCCT 
TCCTCTCCGT CTTCAGCCAC ACCACATAGC CAGAAGCTGG AGCACCTCCA TTGGCCTGTG 
TTCAGCGACC TTGATAACAT GGCACCTCGA GACCAGGACC CTCTGGACAA CCACCGGGAC 
TCTGAGAACG GTGGGGACAG TGGATACCCC AGTGAGAAGC GGGGTGACCT GGATGACCCA 
GAGCCCAGAG AACATGGCCA CTCAAACGGT AACCGGAGGT ACGAGTCGGA TGAAGACAGC 
CTGGGCAGCT CCGGACGGGT CTGTGTGGAG AAGTGGAATC TCTTCAACTC CTCCCGCCTC 
CACCTGCCGA GACCTTCGGC CGTGGCCCTG GAAGTGCAGA GGCTCAACGC TCTGGACCTC 
GAGAAGAAAA TCGGGAAGTC CATTTTGGGG AAGGTCCATC TGGCCATGGT GCGCTACCAC 
GAGGCTGGCC GCTTCTGCGA GAAGGACGAG GAGTGGGACC GGGAGTCGGC GGTCTTCCAC 
CTGGAGCACG CAGCTGACCT GGGCGAGCTG GAGGCCATCG TGGGCCTGGG ACTCATGTAC 
TCGCAGCTGC CCCACCACAT CCTGGCGGAT GTCTCTCTGA AGGAGACAGA AGAGAGCAAA 
ACCAAAGGAT TTGATTACTT ACTGAAGGCA GCTGAAGCTG GGGACAGACA GTGCATGATT 
CTGGTGGCAC GAGCCTTTGA CACTGGCCAG AACCTCAGCC CTGACAGGTC CCAGAGCTGG 
CCAGAGGCTC TCCACTGGTA CAACACTGCC CTGGAGACAA CTGACTGTGA TGAGGGTGGG 
GAGTATGATG GGATGCAAGA CGAACCCCGG TACATGCTGC TGGCCAGGGA GGCGGAGATG 
CTGTTCACAG GCGGCTGCGG GCTGGAGAAG GACCCACAGA GATCAGGCGA TTTGTACACC 
CAGGCAGCCG AGGCAGCGAT GGAAGCCATG AAGGGCCGGC TGGCCAACCA GTACTACGAG 
AAAGCAGAGG AGGCCTGGGC CCAGATGGAG GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010817607.1
CDS338..2233
Translation

Target ORF information:

RefSeq Version XM_010819305.1
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010819305.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCTGACC CCTGGGCTGA GTTCCACCTG GAAGACGTTG CCACGGAATG TGCCACACGC 
CACAGGTACA ACGCTGTTAC CGGCGAGTGG GTGGAGGATG AAGTCCTAAT CAAAATGGCA 
TCCCAGCCCT TCGGCCGGGG AGCAATGAGG GAGTGCTTCA GAACGAAGAA GCTCTCCAAC 
TTCCTGCACA CCCAGCAGTG GAAGGGGGCC TCCAACTACG TGGCAAAGCG CTACATCGAG 
TCCGTGGACA GGGACGTGTA CTTCGAGGAC GTGCGTCTAC AGATGGAGGC CAAGCTCTGG 
GGAGAGGAGT ATAATCGGCA CAAGCCCCCC AAGCAGGTGG ACATCATGCA GATGTGCATC 
ATAGAGCTGA AGGAGAGACC GGGCCGGCCG CTATTCCACC TGGAACACTA CATCGAGGGC 
AAGTACATCA AATATAACTC CAACTCAGGC TTCGTCCGTG ATGACAACGT GCGCCTGACG 
CCACAGGCCT TCAGCCACTT CACGTTTGAG CGTTCCGGCC ACCAGCTGAT TGTGGTGGAC 
ATCCAGGGCG TGGGTGACCT CTATACCGAC CCACAGATCC ACACTGAGAG GGGCACTGAC 
TTTGGAGATG GAAACCTAGG TGTCCGCGGG ATGGCTCTCT TCTTCCACTC ACATGCCTGC 
AACCGGATTT GCAAGAGCAT GGGCCTCACT CCCTTTGACC TGTCCCCGAG AGAGAGGGAT 
GCAGTGAATC AGAACACCAA GCTGCTGCAG TCAGCCAAGA CCATCTTAAG GGGGACAGAG 
GAAAAGTGCG GGAGCCCCCG GGTGAGGACC CTCTCAGGGA GCCGGCCCCC ACTGCTGCTT 
CGCCTCTCAG AGAACTCCGG AGATGACAAC ATGAGCGACG TGACCTTTGA CTCTCTCCCT 
TCCTCTCCGT CTTCAGCCAC ACCACATAGC CAGAAGCTGG AGCACCTCCA TTGGCCTGTG 
TTCAGCGACC TTGATAACAT GGCACCTCGA GACCAGGACC CTCTGGACAA CCACCGGGAC 
TCTGAGAACG GTGGGGACAG TGGATACCCC AGTGAGAAGC GGGGTGACCT GGATGACCCA 
GAGCCCAGAG AACATGGCCA CTCAAACGGT AACCGGAGGT ACGAGTCGGA TGAAGACAGC 
CTGGGCAGCT CCGGACGGGT CTGTGTGGAG AAGTGGAATC TCTTCAACTC CTCCCGCCTC 
CACCTGCCGA GACCTTCGGC CGTGGCCCTG GAAGTGCAGA GGCTCAACGC TCTGGACCTC 
GAGAAGAAAA TCGGGAAGTC CATTTTGGGG AAGGTCCATC TGGCCATGGT GCGCTACCAC 
GAGGCTGGCC GCTTCTGCGA GAAGGACGAG GAGTGGGACC GGGAGTCGGC GGTCTTCCAC 
CTGGAGCACG CAGCTGACCT GGGCGAGCTG GAGGCCATCG TGGGCCTGGG ACTCATGTAC 
TCGCAGCTGC CCCACCACAT CCTGGCGGAT GTCTCTCTGA AGGAGACAGA AGAGAGCAAA 
ACCAAAGGAT TTGATTACTT ACTGAAGGCA GCTGAAGCTG GGGACAGACA GTGCATGATT 
CTGGTGGCAC GAGCCTTTGA CACTGGCCAG AACCTCAGCC CTGACAGGTC CCAGAGCTGG 
CCAGAGGCTC TCCACTGGTA CAACACTGCC CTGGAGACAA CTGACTGTGA TGAGGGTGGG 
GAGTATGATG GGATGCAAGA CGAACCCCGG TACATGCTGC TGGCCAGGGA GGCGGAGATG 
CTGTTCACAG GCGGCTGCGG GCTGGAGAAG GACCCACAGA GATCAGGCGA TTTGTACACC 
CAGGCAGCCG AGGCAGCGAT GGAAGCCATG AAGGGCCGGC TGGCCAACCA GTACTACGAG 
AAAGCAGAGG AGGCCTGGGC CCAGATGGAG GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa50453
Clone ID Related Accession (Same CDS sequence) XM_010819305.1 , XM_010819305.2
Accession Version XM_010819305.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product eukaryotic elongation factor 2 kinase isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819305.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCTGACC CCTGGGCTGA GTTCCACCTG GAAGACGTTG CCACGGAATG TGCCACACGC 
CACAGGTACA ACGCTGTTAC CGGCGAGTGG GTGGAGGATG AAGTCCTAAT CAAAATGGCA 
TCCCAGCCCT TCGGCCGGGG AGCAATGAGG GAGTGCTTCA GAACGAAGAA GCTCTCCAAC 
TTCCTGCACA CCCAGCAGTG GAAGGGGGCC TCCAACTACG TGGCAAAGCG CTACATCGAG 
TCCGTGGACA GGGACGTGTA CTTCGAGGAC GTGCGTCTAC AGATGGAGGC CAAGCTCTGG 
GGAGAGGAGT ATAATCGGCA CAAGCCCCCC AAGCAGGTGG ACATCATGCA GATGTGCATC 
ATAGAGCTGA AGGAGAGACC GGGCCGGCCG CTATTCCACC TGGAACACTA CATCGAGGGC 
AAGTACATCA AATATAACTC CAACTCAGGC TTCGTCCGTG ATGACAACGT GCGCCTGACG 
CCACAGGCCT TCAGCCACTT CACGTTTGAG CGTTCCGGCC ACCAGCTGAT TGTGGTGGAC 
ATCCAGGGCG TGGGTGACCT CTATACCGAC CCACAGATCC ACACTGAGAG GGGCACTGAC 
TTTGGAGATG GAAACCTAGG TGTCCGCGGG ATGGCTCTCT TCTTCCACTC ACATGCCTGC 
AACCGGATTT GCAAGAGCAT GGGCCTCACT CCCTTTGACC TGTCCCCGAG AGAGAGGGAT 
GCAGTGAATC AGAACACCAA GCTGCTGCAG TCAGCCAAGA CCATCTTAAG GGGGACAGAG 
GAAAAGTGCG GGAGCCCCCG GGTGAGGACC CTCTCAGGGA GCCGGCCCCC ACTGCTGCTT 
CGCCTCTCAG AGAACTCCGG AGATGACAAC ATGAGCGACG TGACCTTTGA CTCTCTCCCT 
TCCTCTCCGT CTTCAGCCAC ACCACATAGC CAGAAGCTGG AGCACCTCCA TTGGCCTGTG 
TTCAGCGACC TTGATAACAT GGCACCTCGA GACCAGGACC CTCTGGACAA CCACCGGGAC 
TCTGAGAACG GTGGGGACAG TGGATACCCC AGTGAGAAGC GGGGTGACCT GGATGACCCA 
GAGCCCAGAG AACATGGCCA CTCAAACGGT AACCGGAGGT ACGAGTCGGA TGAAGACAGC 
CTGGGCAGCT CCGGACGGGT CTGTGTGGAG AAGTGGAATC TCTTCAACTC CTCCCGCCTC 
CACCTGCCGA GACCTTCGGC CGTGGCCCTG GAAGTGCAGA GGCTCAACGC TCTGGACCTC 
GAGAAGAAAA TCGGGAAGTC CATTTTGGGG AAGGTCCATC TGGCCATGGT GCGCTACCAC 
GAGGCTGGCC GCTTCTGCGA GAAGGACGAG GAGTGGGACC GGGAGTCGGC GGTCTTCCAC 
CTGGAGCACG CAGCTGACCT GGGCGAGCTG GAGGCCATCG TGGGCCTGGG ACTCATGTAC 
TCGCAGCTGC CCCACCACAT CCTGGCGGAT GTCTCTCTGA AGGAGACAGA AGAGAGCAAA 
ACCAAAGGAT TTGATTACTT ACTGAAGGCA GCTGAAGCTG GGGACAGACA GTGCATGATT 
CTGGTGGCAC GAGCCTTTGA CACTGGCCAG AACCTCAGCC CTGACAGGTC CCAGAGCTGG 
CCAGAGGCTC TCCACTGGTA CAACACTGCC CTGGAGACAA CTGACTGTGA TGAGGGTGGG 
GAGTATGATG GGATGCAAGA CGAACCCCGG TACATGCTGC TGGCCAGGGA GGCGGAGATG 
CTGTTCACAG GCGGCTGCGG GCTGGAGAAG GACCCACAGA GATCAGGCGA TTTGTACACC 
CAGGCAGCCG AGGCAGCGAT GGAAGCCATG AAGGGCCGGC TGGCCAACCA GTACTACGAG 
AAAGCAGAGG AGGCCTGGGC CCAGATGGAG GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010817607.1
CDS359..2254
Translation

Target ORF information:

RefSeq Version XM_010819305.2
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010819305.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCTGACC CCTGGGCTGA GTTCCACCTG GAAGACGTTG CCACGGAATG TGCCACACGC 
CACAGGTACA ACGCTGTTAC CGGCGAGTGG GTGGAGGATG AAGTCCTAAT CAAAATGGCA 
TCCCAGCCCT TCGGCCGGGG AGCAATGAGG GAGTGCTTCA GAACGAAGAA GCTCTCCAAC 
TTCCTGCACA CCCAGCAGTG GAAGGGGGCC TCCAACTACG TGGCAAAGCG CTACATCGAG 
TCCGTGGACA GGGACGTGTA CTTCGAGGAC GTGCGTCTAC AGATGGAGGC CAAGCTCTGG 
GGAGAGGAGT ATAATCGGCA CAAGCCCCCC AAGCAGGTGG ACATCATGCA GATGTGCATC 
ATAGAGCTGA AGGAGAGACC GGGCCGGCCG CTATTCCACC TGGAACACTA CATCGAGGGC 
AAGTACATCA AATATAACTC CAACTCAGGC TTCGTCCGTG ATGACAACGT GCGCCTGACG 
CCACAGGCCT TCAGCCACTT CACGTTTGAG CGTTCCGGCC ACCAGCTGAT TGTGGTGGAC 
ATCCAGGGCG TGGGTGACCT CTATACCGAC CCACAGATCC ACACTGAGAG GGGCACTGAC 
TTTGGAGATG GAAACCTAGG TGTCCGCGGG ATGGCTCTCT TCTTCCACTC ACATGCCTGC 
AACCGGATTT GCAAGAGCAT GGGCCTCACT CCCTTTGACC TGTCCCCGAG AGAGAGGGAT 
GCAGTGAATC AGAACACCAA GCTGCTGCAG TCAGCCAAGA CCATCTTAAG GGGGACAGAG 
GAAAAGTGCG GGAGCCCCCG GGTGAGGACC CTCTCAGGGA GCCGGCCCCC ACTGCTGCTT 
CGCCTCTCAG AGAACTCCGG AGATGACAAC ATGAGCGACG TGACCTTTGA CTCTCTCCCT 
TCCTCTCCGT CTTCAGCCAC ACCACATAGC CAGAAGCTGG AGCACCTCCA TTGGCCTGTG 
TTCAGCGACC TTGATAACAT GGCACCTCGA GACCAGGACC CTCTGGACAA CCACCGGGAC 
TCTGAGAACG GTGGGGACAG TGGATACCCC AGTGAGAAGC GGGGTGACCT GGATGACCCA 
GAGCCCAGAG AACATGGCCA CTCAAACGGT AACCGGAGGT ACGAGTCGGA TGAAGACAGC 
CTGGGCAGCT CCGGACGGGT CTGTGTGGAG AAGTGGAATC TCTTCAACTC CTCCCGCCTC 
CACCTGCCGA GACCTTCGGC CGTGGCCCTG GAAGTGCAGA GGCTCAACGC TCTGGACCTC 
GAGAAGAAAA TCGGGAAGTC CATTTTGGGG AAGGTCCATC TGGCCATGGT GCGCTACCAC 
GAGGCTGGCC GCTTCTGCGA GAAGGACGAG GAGTGGGACC GGGAGTCGGC GGTCTTCCAC 
CTGGAGCACG CAGCTGACCT GGGCGAGCTG GAGGCCATCG TGGGCCTGGG ACTCATGTAC 
TCGCAGCTGC CCCACCACAT CCTGGCGGAT GTCTCTCTGA AGGAGACAGA AGAGAGCAAA 
ACCAAAGGAT TTGATTACTT ACTGAAGGCA GCTGAAGCTG GGGACAGACA GTGCATGATT 
CTGGTGGCAC GAGCCTTTGA CACTGGCCAG AACCTCAGCC CTGACAGGTC CCAGAGCTGG 
CCAGAGGCTC TCCACTGGTA CAACACTGCC CTGGAGACAA CTGACTGTGA TGAGGGTGGG 
GAGTATGATG GGATGCAAGA CGAACCCCGG TACATGCTGC TGGCCAGGGA GGCGGAGATG 
CTGTTCACAG GCGGCTGCGG GCTGGAGAAG GACCCACAGA GATCAGGCGA TTTGTACACC 
CAGGCAGCCG AGGCAGCGAT GGAAGCCATG AAGGGCCGGC TGGCCAACCA GTACTACGAG 
AAAGCAGAGG AGGCCTGGGC CCAGATGGAG GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa32973
Clone ID Related Accession (Same CDS sequence) NM_001192542.1 , XM_005224739.3 , XM_005224739.4
Accession Version NM_001192542.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-28
Organism Bos taurus(cattle)
Product eukaryotic elongation factor 2 kinase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On Jul 17, 2010 this sequence version replaced XM_599997.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.3876.1, SRR5721697.2246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG 
AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT 
GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA 
CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA 
CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA 
TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA 
AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT 
GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG 
GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT 
TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG 
GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC 
GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG 
GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG 
AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT 
GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC 
ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA 
CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG 
GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA 
GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001179471.1
CDS149..2320
Translation

Target ORF information:

RefSeq Version NM_001192542.1
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001192542.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG 
AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT 
GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA 
CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA 
CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA 
TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA 
AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT 
GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG 
GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT 
TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG 
GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC 
GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG 
GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG 
AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT 
GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC 
ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA 
CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG 
GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA 
GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa32973
Clone ID Related Accession (Same CDS sequence) NM_001192542.1 , XM_005224739.3 , XM_005224739.4
Accession Version XM_005224739.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product eukaryotic elongation factor 2 kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224739.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG 
AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT 
GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA 
CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA 
CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA 
TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA 
AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT 
GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG 
GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT 
TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG 
GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC 
GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG 
GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG 
AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT 
GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC 
ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA 
CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG 
GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA 
GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005224796.1
CDS178..2349
Translation

Target ORF information:

RefSeq Version XM_005224739.3
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005224739.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG 
AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT 
GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA 
CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA 
CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA 
TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA 
AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT 
GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG 
GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT 
TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG 
GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC 
GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG 
GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG 
AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT 
GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC 
ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA 
CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG 
GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA 
GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa32973
Clone ID Related Accession (Same CDS sequence) NM_001192542.1 , XM_005224739.3 , XM_005224739.4
Accession Version XM_005224739.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product eukaryotic elongation factor 2 kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224739.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG 
AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT 
GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA 
CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA 
CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA 
TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA 
AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT 
GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG 
GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT 
TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG 
GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC 
GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG 
GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG 
AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT 
GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC 
ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA 
CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG 
GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA 
GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005224796.1
CDS481..2652
Translation

Target ORF information:

RefSeq Version XM_005224739.4
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005224739.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG 
AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT 
GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA 
CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA 
CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA 
TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA 
AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT 
GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG 
GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT 
TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG 
GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC 
GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG 
GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG 
AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT 
GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC 
ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA 
CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG 
GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA 
GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa32974
Clone ID Related Accession (Same CDS sequence) XM_005224740.3 , XM_005224740.4
Accession Version XM_005224740.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product eukaryotic elongation factor 2 kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGTCAGCCA AGACCATCTT AAGGGGGACA GAGGAAAAGT GCGGGAGCCC CCGGGTGAGG 
ACCCTCTCAG GGAGCCGGCC CCCACTGCTG CTTCGCCTCT CAGAGAACTC CGGAGATGAC 
AACATGAGCG ACGTGACCTT TGACTCTCTC CCTTCCTCTC CGTCTTCAGC CACACCACAT 
AGCCAGAAGC TGGAGCACCT CCATTGGCCT GTGTTCAGCG ACCTTGATAA CATGGCACCT 
CGAGACCAGG ACCCTCTGGA CAACCACCGG GACTCTGAGA ACGGTGGGGA CAGTGGATAC 
CCCAGTGAGA AGCGGGGTGA CCTGGATGAC CCAGAGCCCA GAGAACATGG CCACTCAAAC 
GGTAACCGGA GGTACGAGTC GGATGAAGAC AGCCTGGGCA GCTCCGGACG GGTCTGTGTG 
GAGAAGTGGA ATCTCTTCAA CTCCTCCCGC CTCCACCTGC CGAGACCTTC GGCCGTGGCC 
CTGGAAGTGC AGAGGCTCAA CGCTCTGGAC CTCGAGAAGA AAATCGGGAA GTCCATTTTG 
GGGAAGGTCC ATCTGGCCAT GGTGCGCTAC CACGAGGCTG GCCGCTTCTG CGAGAAGGAC 
GAGGAGTGGG ACCGGGAGTC GGCGGTCTTC CACCTGGAGC ACGCAGCTGA CCTGGGCGAG 
CTGGAGGCCA TCGTGGGCCT GGGACTCATG TACTCGCAGC TGCCCCACCA CATCCTGGCG 
GATGTCTCTC TGAAGGAGAC AGAAGAGAGC AAAACCAAAG GATTTGATTA CTTACTGAAG 
GCAGCTGAAG CTGGGGACAG ACAGTGCATG ATTCTGGTGG CACGAGCCTT TGACACTGGC 
CAGAACCTCA GCCCTGACAG GTCCCAGAGC TGGCCAGAGG CTCTCCACTG GTACAACACT 
GCCCTGGAGA CAACTGACTG TGATGAGGGT GGGGAGTATG ATGGGATGCA AGACGAACCC 
CGGTACATGC TGCTGGCCAG GGAGGCGGAG ATGCTGTTCA CAGGCGGCTG CGGGCTGGAG 
AAGGACCCAC AGAGATCAGG CGATTTGTAC ACCCAGGCAG CCGAGGCAGC GATGGAAGCC 
ATGAAGGGCC GGCTGGCCAA CCAGTACTAC GAGAAAGCAG AGGAGGCCTG GGCCCAGATG 
GAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005224797.1
CDS180..2348
Translation

Target ORF information:

RefSeq Version XM_005224740.3
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005224740.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGTCAGCCA AGACCATCTT AAGGGGGACA GAGGAAAAGT GCGGGAGCCC CCGGGTGAGG 
ACCCTCTCAG GGAGCCGGCC CCCACTGCTG CTTCGCCTCT CAGAGAACTC CGGAGATGAC 
AACATGAGCG ACGTGACCTT TGACTCTCTC CCTTCCTCTC CGTCTTCAGC CACACCACAT 
AGCCAGAAGC TGGAGCACCT CCATTGGCCT GTGTTCAGCG ACCTTGATAA CATGGCACCT 
CGAGACCAGG ACCCTCTGGA CAACCACCGG GACTCTGAGA ACGGTGGGGA CAGTGGATAC 
CCCAGTGAGA AGCGGGGTGA CCTGGATGAC CCAGAGCCCA GAGAACATGG CCACTCAAAC 
GGTAACCGGA GGTACGAGTC GGATGAAGAC AGCCTGGGCA GCTCCGGACG GGTCTGTGTG 
GAGAAGTGGA ATCTCTTCAA CTCCTCCCGC CTCCACCTGC CGAGACCTTC GGCCGTGGCC 
CTGGAAGTGC AGAGGCTCAA CGCTCTGGAC CTCGAGAAGA AAATCGGGAA GTCCATTTTG 
GGGAAGGTCC ATCTGGCCAT GGTGCGCTAC CACGAGGCTG GCCGCTTCTG CGAGAAGGAC 
GAGGAGTGGG ACCGGGAGTC GGCGGTCTTC CACCTGGAGC ACGCAGCTGA CCTGGGCGAG 
CTGGAGGCCA TCGTGGGCCT GGGACTCATG TACTCGCAGC TGCCCCACCA CATCCTGGCG 
GATGTCTCTC TGAAGGAGAC AGAAGAGAGC AAAACCAAAG GATTTGATTA CTTACTGAAG 
GCAGCTGAAG CTGGGGACAG ACAGTGCATG ATTCTGGTGG CACGAGCCTT TGACACTGGC 
CAGAACCTCA GCCCTGACAG GTCCCAGAGC TGGCCAGAGG CTCTCCACTG GTACAACACT 
GCCCTGGAGA CAACTGACTG TGATGAGGGT GGGGAGTATG ATGGGATGCA AGACGAACCC 
CGGTACATGC TGCTGGCCAG GGAGGCGGAG ATGCTGTTCA CAGGCGGCTG CGGGCTGGAG 
AAGGACCCAC AGAGATCAGG CGATTTGTAC ACCCAGGCAG CCGAGGCAGC GATGGAAGCC 
ATGAAGGGCC GGCTGGCCAA CCAGTACTAC GAGAAAGCAG AGGAGGCCTG GGCCCAGATG 
GAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa32974
Clone ID Related Accession (Same CDS sequence) XM_005224740.3 , XM_005224740.4
Accession Version XM_005224740.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product eukaryotic elongation factor 2 kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224740.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGTCAGCCA AGACCATCTT AAGGGGGACA GAGGAAAAGT GCGGGAGCCC CCGGGTGAGG 
ACCCTCTCAG GGAGCCGGCC CCCACTGCTG CTTCGCCTCT CAGAGAACTC CGGAGATGAC 
AACATGAGCG ACGTGACCTT TGACTCTCTC CCTTCCTCTC CGTCTTCAGC CACACCACAT 
AGCCAGAAGC TGGAGCACCT CCATTGGCCT GTGTTCAGCG ACCTTGATAA CATGGCACCT 
CGAGACCAGG ACCCTCTGGA CAACCACCGG GACTCTGAGA ACGGTGGGGA CAGTGGATAC 
CCCAGTGAGA AGCGGGGTGA CCTGGATGAC CCAGAGCCCA GAGAACATGG CCACTCAAAC 
GGTAACCGGA GGTACGAGTC GGATGAAGAC AGCCTGGGCA GCTCCGGACG GGTCTGTGTG 
GAGAAGTGGA ATCTCTTCAA CTCCTCCCGC CTCCACCTGC CGAGACCTTC GGCCGTGGCC 
CTGGAAGTGC AGAGGCTCAA CGCTCTGGAC CTCGAGAAGA AAATCGGGAA GTCCATTTTG 
GGGAAGGTCC ATCTGGCCAT GGTGCGCTAC CACGAGGCTG GCCGCTTCTG CGAGAAGGAC 
GAGGAGTGGG ACCGGGAGTC GGCGGTCTTC CACCTGGAGC ACGCAGCTGA CCTGGGCGAG 
CTGGAGGCCA TCGTGGGCCT GGGACTCATG TACTCGCAGC TGCCCCACCA CATCCTGGCG 
GATGTCTCTC TGAAGGAGAC AGAAGAGAGC AAAACCAAAG GATTTGATTA CTTACTGAAG 
GCAGCTGAAG CTGGGGACAG ACAGTGCATG ATTCTGGTGG CACGAGCCTT TGACACTGGC 
CAGAACCTCA GCCCTGACAG GTCCCAGAGC TGGCCAGAGG CTCTCCACTG GTACAACACT 
GCCCTGGAGA CAACTGACTG TGATGAGGGT GGGGAGTATG ATGGGATGCA AGACGAACCC 
CGGTACATGC TGCTGGCCAG GGAGGCGGAG ATGCTGTTCA CAGGCGGCTG CGGGCTGGAG 
AAGGACCCAC AGAGATCAGG CGATTTGTAC ACCCAGGCAG CCGAGGCAGC GATGGAAGCC 
ATGAAGGGCC GGCTGGCCAA CCAGTACTAC GAGAAAGCAG AGGAGGCCTG GGCCCAGATG 
GAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005224797.1
CDS400..2568
Translation

Target ORF information:

RefSeq Version XM_005224740.4
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005224740.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC 
ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC 
CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG 
GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC 
CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC 
GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA 
ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG 
GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC 
GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG 
CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC 
CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC 
TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG 
TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT 
ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC 
CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC 
CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG 
CTGTCAGCCA AGACCATCTT AAGGGGGACA GAGGAAAAGT GCGGGAGCCC CCGGGTGAGG 
ACCCTCTCAG GGAGCCGGCC CCCACTGCTG CTTCGCCTCT CAGAGAACTC CGGAGATGAC 
AACATGAGCG ACGTGACCTT TGACTCTCTC CCTTCCTCTC CGTCTTCAGC CACACCACAT 
AGCCAGAAGC TGGAGCACCT CCATTGGCCT GTGTTCAGCG ACCTTGATAA CATGGCACCT 
CGAGACCAGG ACCCTCTGGA CAACCACCGG GACTCTGAGA ACGGTGGGGA CAGTGGATAC 
CCCAGTGAGA AGCGGGGTGA CCTGGATGAC CCAGAGCCCA GAGAACATGG CCACTCAAAC 
GGTAACCGGA GGTACGAGTC GGATGAAGAC AGCCTGGGCA GCTCCGGACG GGTCTGTGTG 
GAGAAGTGGA ATCTCTTCAA CTCCTCCCGC CTCCACCTGC CGAGACCTTC GGCCGTGGCC 
CTGGAAGTGC AGAGGCTCAA CGCTCTGGAC CTCGAGAAGA AAATCGGGAA GTCCATTTTG 
GGGAAGGTCC ATCTGGCCAT GGTGCGCTAC CACGAGGCTG GCCGCTTCTG CGAGAAGGAC 
GAGGAGTGGG ACCGGGAGTC GGCGGTCTTC CACCTGGAGC ACGCAGCTGA CCTGGGCGAG 
CTGGAGGCCA TCGTGGGCCT GGGACTCATG TACTCGCAGC TGCCCCACCA CATCCTGGCG 
GATGTCTCTC TGAAGGAGAC AGAAGAGAGC AAAACCAAAG GATTTGATTA CTTACTGAAG 
GCAGCTGAAG CTGGGGACAG ACAGTGCATG ATTCTGGTGG CACGAGCCTT TGACACTGGC 
CAGAACCTCA GCCCTGACAG GTCCCAGAGC TGGCCAGAGG CTCTCCACTG GTACAACACT 
GCCCTGGAGA CAACTGACTG TGATGAGGGT GGGGAGTATG ATGGGATGCA AGACGAACCC 
CGGTACATGC TGCTGGCCAG GGAGGCGGAG ATGCTGTTCA CAGGCGGCTG CGGGCTGGAG 
AAGGACCCAC AGAGATCAGG CGATTTGTAC ACCCAGGCAG CCGAGGCAGC GATGGAAGCC 
ATGAAGGGCC GGCTGGCCAA CCAGTACTAC GAGAAAGCAG AGGAGGCCTG GGCCCAGATG 
GAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL