CDC42BPA gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024976359.1	XP_024832127.1	serine/threonine-protein kinase MRCK alpha isoform X5
XM_024976360.1	XP_024832128.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_024976361.1	XP_024832129.1	serine/threonine-protein kinase MRCK alpha isoform X7
XM_024976354.1	XP_024832122.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_024976358.1	XP_024832126.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_024976363.1	XP_024832131.1	serine/threonine-protein kinase MRCK alpha isoform X9
XM_024976357.1	XP_024832125.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_024976355.1	XP_024832123.1	serine/threonine-protein kinase MRCK alpha isoform X2
NM_001192937.1	NP_001179866.1	serine/threonine-protein kinase MRCK alpha
XM_024976362.1	XP_024832130.1	serine/threonine-protein kinase MRCK alpha isoform X8
NM_001192937.2	NP_001179866.2	serine/threonine-protein kinase MRCK alpha

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa239064	NM_001192937.2 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223662	XM_024976354.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223663	XM_024976355.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223664	XM_024976357.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223665	XM_024976358.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223666	XM_024976359.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223667	XM_024976360.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223668	XM_024976361.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223669	XM_024976362.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223670	XM_024976363.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa37050	NM_001192937.1 Latest version!	Bos taurus CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

CDC42BPA ( NM_001192937.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179866.2 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), mRNA.

Price & Availability↑

CloneID	OBa239064
Clone ID Related Accession (Same CDS sequence)	NM_001192937.2
Accession Version	NM_001192937.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On May 30, 2018 this sequence version replaced NM_001192937.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCCGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCAGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCTTCGGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCA CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC TAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCAGTGTCG 
CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179866.2
CDS	263..5422
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001192937.2
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192937.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCCGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCAGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCTTCGGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCA CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC TAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCAGTGTCG 
CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_024976354.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832122.1 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

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CloneID	OBa223662
Clone ID Related Accession (Same CDS sequence)	XM_024976354.1
Accession Version	XM_024976354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG 
TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT 
ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG TTCAACTGGC 
TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAAG 
TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG 
TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT 
GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG 
GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG 
CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT 
GAAAATGAGA AGAGTAAGTG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT 
GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA 
CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGAAAAGT GCGTCATGGA 
GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CATACAACGT CCAGTGGATG 
GCAATCTTCA GTGAGCAACT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG 
AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT 
GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG 
TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC 
AGTGAAAATG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG 
ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA 
ACATCAGATA ATAGTCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT 
TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA 
ATGAGAAATA AGTTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCGG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCCGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG CATTCAGTGG CTCCGTCAGT ATTCCGTCTA TCACCAAATC CCGCCCTGAA 
CCCGGTCGCT CCATGAGCGC CAGCAGTGGC CTATCAGCAA GGTCATCTGC ACAGAATGGC 
AGTGCGTTAA AGAGAGAATT CTCTGGAGGC AGCTACAGTG CAAAGCGGCA GCCCATGCCC 
TCCCCATCGG AGGGCTCGCT GTCTTCGGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCA 
CGGGACTATG ACGGAGAGGA CTCTGATTCT CCGCGGCATT CCACGGCTTC TAACAGTTCC 
AACCTGAGCA GTCCTCCCAG CCCAGTGTCG CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG 
AGCACAGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832122.1
CDS	1282..6771
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_024976354.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976354.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG 
TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT 
ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG TTCAACTGGC 
TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAAG 
TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG 
TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT 
GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG 
GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG 
CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT 
GAAAATGAGA AGAGTAAGTG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT 
GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA 
CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGAAAAGT GCGTCATGGA 
GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CATACAACGT CCAGTGGATG 
GCAATCTTCA GTGAGCAACT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG 
AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT 
GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG 
TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC 
AGTGAAAATG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG 
ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA 
ACATCAGATA ATAGTCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT 
TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA 
ATGAGAAATA AGTTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCGG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCCGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG CATTCAGTGG CTCCGTCAGT ATTCCGTCTA TCACCAAATC CCGCCCTGAA 
CCCGGTCGCT CCATGAGCGC CAGCAGTGGC CTATCAGCAA GGTCATCTGC ACAGAATGGC 
AGTGCGTTAA AGAGAGAATT CTCTGGAGGC AGCTACAGTG CAAAGCGGCA GCCCATGCCC 
TCCCCATCGG AGGGCTCGCT GTCTTCGGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCA 
CGGGACTATG ACGGAGAGGA CTCTGATTCT CCGCGGCATT CCACGGCTTC TAACAGTTCC 
AACCTGAGCA GTCCTCCCAG CCCAGTGTCG CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG 
AGCACAGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_024976355.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832123.1 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

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CloneID	OBa223663
Clone ID Related Accession (Same CDS sequence)	XM_024976355.1
Accession Version	XM_024976355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GTTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAA GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAGT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGAAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCATACAACG TCCAGTGGAT GGCAATCTTC AGTGAGCAAC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAT GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCAGAT AATAGTCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAGTTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCCGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCCGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCAGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCTTCGGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC ACGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CTAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCAGTGTC GCCCCGGAAG 
ACCAAGAGCC TATCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832123.1
CDS	1282..6732
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_024976355.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976355.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GTTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAA GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAGT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGAAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCATACAACG TCCAGTGGAT GGCAATCTTC AGTGAGCAAC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAT GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCAGAT AATAGTCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAGTTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCCGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCCGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCAGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCTTCGGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC ACGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CTAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCAGTGTC GCCCCGGAAG 
ACCAAGAGCC TATCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_024976357.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832125.1 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

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CloneID	OBa223664
Clone ID Related Accession (Same CDS sequence)	XM_024976357.1
Accession Version	XM_024976357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCTACTCCA CTTCCAGTTC ATACACCAAC CTTAAGGAAA 
AAGGGATGCC CTGGTTCAAC TGGCTTTCCA TCGAAGCGCA AGATTCATCA GTTTTTTGTC 
AAATCTTTTA CTACTCCCAC CAAGTGTCAT CAGTGTACAT CTTTGATGGT GGGTTTGATA 
AGACAAGGCT GTTCTTGTGA AGTGTGTGGA TTTTCATGTC ACATAACTTG TGTAAACAAA 
GCTCCAACTG CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCTCTGGG TATAGATCCA 
CAGAAAGGAA TAGGAACAGC ATATGAAGGG CATGTCAGGA TCCCTAAGCC AGCTGGAGTG 
AAGAAAGGAT GGCAAAGAGC ACTGGCTGTA GTCTGTGACT TCAAGCTCTT TCTGTACGAT 
ATTGCCGAAG GAAAGGCATC TCAGCCCAGT GTTGTAGTCA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA 
GATATACCCT GTATATTTAG AGTCACAGCT TCCCAGCTCT CAGCATCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCTGA TAGTGAAAAT GAGAAGAGTA AGTGGGTGGG AGTACTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT 
CATGAAAGAA TAGCTTTGGG GAATGAAGAA GGGTTATTTG TTGTGCATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TAGAACTCAT TCCAAATGAT 
CAACTTGTTG CCGTGATCTC AGGACGAAAT CGACACGTCC GACTTTTTCC TATGTCAGCT 
CTGGACGGGC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGCTG TCAAACAATA 
ACTTCTGGAA AAGTGCGTCA TGGAGCTCTT ACATGCTTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACGCGTCACA GAAAATTTAA GGAAATTCAA 
GTCCCATACA ACGTCCAGTG GATGGCAATC TTCAGTGAGC AACTCTGTGT GGGATTCCAG 
TCTGGATTTC TAAGATATCC CCTGAATGGA GAAGGAAGTC CATACAGTAT GCTCCATTCA 
AATGACAATA CACTCTCATT TATTGCACAT CAACCAATGG ATGCCATCTG TGCGGTTGAA 
ATCTCCAGTA AAGAATACCT GCTGTGTTTT AACAGCATTG GAATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAACTGATG TGGCCAGCGA ATCCTTCCTC TTGTTGTTAC 
AATGCACCGT ATCTCTCAGT GTACAGTGAA AATGCAGTTG ACATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGCC CCTTAAACAG TGAAGGATCA 
TTAAATCTTT TAGGCTTGGA GACGATTAGA TTGATATATT TCAAAAATAA AATGGCAGAA 
GGGGATGAAC TGGTAGTTCC TGAAACATCA GATAATAGTC GGAAGCAAAT GGTTAGAAAT 
ATTAACAATA AGAGGCGTTA CTCTTTCAGA GTTCCGGAAG AGGAAAGAAT GCAACAGAGG 
AGGGAGATGT TACGAGATCC AGAAATGAGA AATAAGTTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACATATGGG TCCAGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCGGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCCG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGCATTCA GTGGCTCCGT CAGTATTCCG 
TCTATCACCA AATCCCGCCC TGAACCCGGT CGCTCCATGA GCGCCAGCAG TGGCCTATCA 
GCAAGGTCAT CTGCACAGAA TGGCAGTGCG TTAAAGAGAG AATTCTCTGG AGGCAGCTAC 
AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA TCGGAGGGCT CGCTGTCTTC GGGAGGCATG 
GACCAGGGAA GTGACGCCCC AGCACGGGAC TATGACGGAG AGGACTCTGA TTCTCCGCGG 
CATTCCACGG CTTCTAACAG TTCCAACCTG AGCAGTCCTC CCAGCCCAGT GTCGCCCCGG 
AAGACCAAGA GCCTATCTCT GGAGAGCACA GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832125.1
CDS	1282..6675
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_024976357.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976357.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCTACTCCA CTTCCAGTTC ATACACCAAC CTTAAGGAAA 
AAGGGATGCC CTGGTTCAAC TGGCTTTCCA TCGAAGCGCA AGATTCATCA GTTTTTTGTC 
AAATCTTTTA CTACTCCCAC CAAGTGTCAT CAGTGTACAT CTTTGATGGT GGGTTTGATA 
AGACAAGGCT GTTCTTGTGA AGTGTGTGGA TTTTCATGTC ACATAACTTG TGTAAACAAA 
GCTCCAACTG CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCTCTGGG TATAGATCCA 
CAGAAAGGAA TAGGAACAGC ATATGAAGGG CATGTCAGGA TCCCTAAGCC AGCTGGAGTG 
AAGAAAGGAT GGCAAAGAGC ACTGGCTGTA GTCTGTGACT TCAAGCTCTT TCTGTACGAT 
ATTGCCGAAG GAAAGGCATC TCAGCCCAGT GTTGTAGTCA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA 
GATATACCCT GTATATTTAG AGTCACAGCT TCCCAGCTCT CAGCATCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCTGA TAGTGAAAAT GAGAAGAGTA AGTGGGTGGG AGTACTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT 
CATGAAAGAA TAGCTTTGGG GAATGAAGAA GGGTTATTTG TTGTGCATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TAGAACTCAT TCCAAATGAT 
CAACTTGTTG CCGTGATCTC AGGACGAAAT CGACACGTCC GACTTTTTCC TATGTCAGCT 
CTGGACGGGC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGCTG TCAAACAATA 
ACTTCTGGAA AAGTGCGTCA TGGAGCTCTT ACATGCTTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACGCGTCACA GAAAATTTAA GGAAATTCAA 
GTCCCATACA ACGTCCAGTG GATGGCAATC TTCAGTGAGC AACTCTGTGT GGGATTCCAG 
TCTGGATTTC TAAGATATCC CCTGAATGGA GAAGGAAGTC CATACAGTAT GCTCCATTCA 
AATGACAATA CACTCTCATT TATTGCACAT CAACCAATGG ATGCCATCTG TGCGGTTGAA 
ATCTCCAGTA AAGAATACCT GCTGTGTTTT AACAGCATTG GAATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAACTGATG TGGCCAGCGA ATCCTTCCTC TTGTTGTTAC 
AATGCACCGT ATCTCTCAGT GTACAGTGAA AATGCAGTTG ACATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGCC CCTTAAACAG TGAAGGATCA 
TTAAATCTTT TAGGCTTGGA GACGATTAGA TTGATATATT TCAAAAATAA AATGGCAGAA 
GGGGATGAAC TGGTAGTTCC TGAAACATCA GATAATAGTC GGAAGCAAAT GGTTAGAAAT 
ATTAACAATA AGAGGCGTTA CTCTTTCAGA GTTCCGGAAG AGGAAAGAAT GCAACAGAGG 
AGGGAGATGT TACGAGATCC AGAAATGAGA AATAAGTTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACATATGGG TCCAGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCGGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCCG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGCATTCA GTGGCTCCGT CAGTATTCCG 
TCTATCACCA AATCCCGCCC TGAACCCGGT CGCTCCATGA GCGCCAGCAG TGGCCTATCA 
GCAAGGTCAT CTGCACAGAA TGGCAGTGCG TTAAAGAGAG AATTCTCTGG AGGCAGCTAC 
AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA TCGGAGGGCT CGCTGTCTTC GGGAGGCATG 
GACCAGGGAA GTGACGCCCC AGCACGGGAC TATGACGGAG AGGACTCTGA TTCTCCGCGG 
CATTCCACGG CTTCTAACAG TTCCAACCTG AGCAGTCCTC CCAGCCCAGT GTCGCCCCGG 
AAGACCAAGA GCCTATCTCT GGAGAGCACA GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_024976358.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832126.1 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

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CloneID	OBa223665
Clone ID Related Accession (Same CDS sequence)	XM_024976358.1
Accession Version	XM_024976358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGTTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAAGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAGTGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGA 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCATAC 
AACGTCCAGT GGATGGCAAT CTTCAGTGAG CAACTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAATGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC AGATAATAGT CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAGTTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCC GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGCATTC AGTGGCTCCG TCAGTATTCC GTCTATCACC 
AAATCCCGCC CTGAACCCGG TCGCTCCATG AGCGCCAGCA GTGGCCTATC AGCAAGGTCA 
TCTGCACAGA ATGGCAGTGC GTTAAAGAGA GAATTCTCTG GAGGCAGCTA CAGTGCAAAG 
CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC TCGCTGTCTT CGGGAGGCAT GGACCAGGGA 
AGTGACGCCC CAGCACGGGA CTATGACGGA GAGGACTCTG ATTCTCCGCG GCATTCCACG 
GCTTCTAACA GTTCCAACCT GAGCAGTCCT CCCAGCCCAG TGTCGCCCCG GAAGACCAAG 
AGCCTATCTC TGGAGAGCAC AGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832126.1
CDS	1282..6666
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_024976358.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976358.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGTTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAAGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAGTGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGA 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCATAC 
AACGTCCAGT GGATGGCAAT CTTCAGTGAG CAACTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAATGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC AGATAATAGT CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAGTTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCC GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGCATTC AGTGGCTCCG TCAGTATTCC GTCTATCACC 
AAATCCCGCC CTGAACCCGG TCGCTCCATG AGCGCCAGCA GTGGCCTATC AGCAAGGTCA 
TCTGCACAGA ATGGCAGTGC GTTAAAGAGA GAATTCTCTG GAGGCAGCTA CAGTGCAAAG 
CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC TCGCTGTCTT CGGGAGGCAT GGACCAGGGA 
AGTGACGCCC CAGCACGGGA CTATGACGGA GAGGACTCTG ATTCTCCGCG GCATTCCACG 
GCTTCTAACA GTTCCAACCT GAGCAGTCCT CCCAGCCCAG TGTCGCCCCG GAAGACCAAG 
AGCCTATCTC TGGAGAGCAC AGACCGCGGG AGCTGGGACC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_024976359.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832127.1 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

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CloneID	OBa223666
Clone ID Related Accession (Same CDS sequence)	XM_024976359.1
Accession Version	XM_024976359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCC 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CAGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCT 
TCGGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCACGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCTAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCA 
GTGTCGCCCC GGAAGACCAA GAGCCTATCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832127.1
CDS	1282..6627
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_024976359.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976359.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCC 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CAGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCT 
TCGGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCACGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCTAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCA 
GTGTCGCCCC GGAAGACCAA GAGCCTATCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_024976360.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832128.1 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

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CloneID	OBa223667
Clone ID Related Accession (Same CDS sequence)	XM_024976360.1
Accession Version	XM_024976360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG 
TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT 
ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG TTCAACTGGC 
TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAAG 
TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG 
TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT 
GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG 
GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG 
CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT 
GAAAATGAGA AGAGTAAGTG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT 
GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA 
CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGAAAAGT GCGTCATGGA 
GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CATACAACGT CCAGTGGATG 
GCAATCTTCA GTGAGCAACT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG 
AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT 
GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG 
TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC 
AGTGAAAATG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG 
ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA 
ACATCAGATA ATAGTCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT 
TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA 
ATGAGAAATA AGTTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGAACC CTCGGCCTCA GGAAAGTCGG 
ACAGCATTCA GTGGCTCCGT CAGTATTCCG TCTATCACCA AATCCCGCCC TGAACCCGGT 
CGCTCCATGA GCGCCAGCAG TGGCCTATCA GCAAGGTCAT CTGCACAGAA TGGCAGTGCG 
TTAAAGAGAG AATTCTCTGG AGGCAGCTAC AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA 
TCGGAGGGCT CGCTGTCTTC GGGAGGCATG GACCAGGGAA GTGACGCCCC AGCACGGGAC 
TATGACGGAG AGGACTCTGA TTCTCCGCGG CATTCCACGG CTTCTAACAG TTCCAACCTG 
AGCAGTCCTC CCAGCCCAGT GTCGCCCCGG AAGACCAAGA GCCTATCTCT GGAGAGCACA 
GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832128.1
CDS	1282..6585
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_024976360.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976360.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG 
TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT 
ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG TTCAACTGGC 
TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAAG 
TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG 
TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT 
GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG 
GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG 
CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT 
GAAAATGAGA AGAGTAAGTG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT 
GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA 
CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGAAAAGT GCGTCATGGA 
GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CATACAACGT CCAGTGGATG 
GCAATCTTCA GTGAGCAACT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG 
AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT 
GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG 
TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC 
AGTGAAAATG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG 
ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA 
ACATCAGATA ATAGTCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT 
TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA 
ATGAGAAATA AGTTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGAACC CTCGGCCTCA GGAAAGTCGG 
ACAGCATTCA GTGGCTCCGT CAGTATTCCG TCTATCACCA AATCCCGCCC TGAACCCGGT 
CGCTCCATGA GCGCCAGCAG TGGCCTATCA GCAAGGTCAT CTGCACAGAA TGGCAGTGCG 
TTAAAGAGAG AATTCTCTGG AGGCAGCTAC AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA 
TCGGAGGGCT CGCTGTCTTC GGGAGGCATG GACCAGGGAA GTGACGCCCC AGCACGGGAC 
TATGACGGAG AGGACTCTGA TTCTCCGCGG CATTCCACGG CTTCTAACAG TTCCAACCTG 
AGCAGTCCTC CCAGCCCAGT GTCGCCCCGG AAGACCAAGA GCCTATCTCT GGAGAGCACA 
GACCGCGGGA GCTGGGACCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_024976361.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832129.1 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

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CloneID	OBa223668
Clone ID Related Accession (Same CDS sequence)	XM_024976361.1
Accession Version	XM_024976361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAACAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA TTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA ACGCTCTCCA TCCTGTACTC CAGCTAGCAA AGGCAGACGT 
ACTGATCCCG TTGAGAACAC ATATGTACGG AATCCGAACG TCAAGTTTTA CATCCAGTCA 
AGGTCCACCT CTCCTTCCAC ATCTAGTGAA GCTGAGCCAG TTAAGACTGT AGACTCTACT 
CCACTTCCAG TTCATACACC AACCTTAAGG AAAAAGGGAT GCCCTGGTTC AACTGGCTTT 
CCATCGAAGC GCAAGATTCA TCAGTTTTTT GTCAAATCTT TTACTACTCC CACCAAGTGT 
CATCAGTGTA CATCTTTGAT GGTGGGTTTG ATAAGACAAG GCTGTTCTTG TGAAGTGTGT 
GGATTTTCAT GTCACATAAC TTGTGTAAAC AAAGCTCCAA CTGCTTGTCC AGTTCCTCCT 
GAACAGACAA AAGGTCCTCT GGGTATAGAT CCACAGAAAG GAATAGGAAC AGCATATGAA 
GGGCATGTCA GGATCCCTAA GCCAGCTGGA GTGAAGAAAG GATGGCAAAG AGCACTGGCT 
GTAGTCTGTG ACTTCAAGCT CTTTCTGTAC GATATTGCCG AAGGAAAGGC ATCTCAGCCC 
AGTGTTGTAG TCAGTCAAGT GATTGACATG AGGGATGAAG AATTTTCTGT GAGTTCAGTC 
TTGGCTTCTG ATGTTATTCA TGCAAGTCGA AAAGATATAC CCTGTATATT TAGAGTCACA 
GCTTCCCAGC TCTCAGCATC TAGTAACAAA TGTTCCATCC TGATGCTAGC TGATAGTGAA 
AATGAGAAGA GTAAGTGGGT GGGAGTACTG AGTGAATTGC ACAAGATTTT GAAGAAAAAC 
AAATTCAGAG ACCGCTCAGT CTATGTTCCC AAAGAGGCTT ATGACAGCAC TCTGCCCCTC 
ATTAAAACAA CCCAGGCAGC TGCAATCATA GATCATGAAA GAATAGCTTT GGGGAATGAA 
GAAGGGTTAT TTGTTGTGCA TGTCACCAAA GATGAAATTA TTAGAGTTGG TGACAATAAG 
AAGATTCATC AGATAGAACT CATTCCAAAT GATCAACTTG TTGCCGTGAT CTCAGGACGA 
AATCGACACG TCCGACTTTT TCCTATGTCA GCTCTGGACG GGCGAGAGAC TGATTTTTAT 
AAGCTGGCAG AAACTAAAGG CTGTCAAACA ATAACTTCTG GAAAAGTGCG TCATGGAGCT 
CTTACATGCT TGTGTGTGGC TATGAAAAGG CAGGTCCTCT GTTATGAACT ATTTCAGAGC 
AAGACGCGTC ACAGAAAATT TAAGGAAATT CAAGTCCCAT ACAACGTCCA GTGGATGGCA 
ATCTTCAGTG AGCAACTCTG TGTGGGATTC CAGTCTGGAT TTCTAAGATA TCCCCTGAAT 
GGAGAAGGAA GTCCATACAG TATGCTCCAT TCAAATGACA ATACACTCTC ATTTATTGCA 
CATCAACCAA TGGATGCCAT CTGTGCGGTT GAAATCTCCA GTAAAGAATA CCTGCTGTGT 
TTTAACAGCA TTGGAATATA CACTGACTGC CAGGGCCGAA GATCTAGACA ACAGGAACTG 
ATGTGGCCAG CGAATCCTTC CTCTTGTTGT TACAATGCAC CGTATCTCTC AGTGTACAGT 
GAAAATGCAG TTGACATCTT TGATGTGAAC TCCATGGAAT GGATTCAGAC TCTCCCACTC 
AAAAAGGTTC GCCCCTTAAA CAGTGAAGGA TCATTAAATC TTTTAGGCTT GGAGACGATT 
AGATTGATAT ATTTCAAAAA TAAAATGGCA GAAGGGGATG AACTGGTAGT TCCTGAAACA 
TCAGATAATA GTCGGAAGCA AATGGTTAGA AATATTAACA ATAAGAGGCG TTACTCTTTC 
AGAGTTCCGG AAGAGGAAAG AATGCAACAG AGGAGGGAGA TGTTACGAGA TCCAGAAATG 
AGAAATAAGT TAATTTCTAA CCCAACTAAT TTTAACCACA TAGCACATAT GGGTCCAGGA 
GATGGAATAC AGATCCTAAA AGACCTGCCC ATGCCGGGTT TCCCTTATCC ATCCCCTCAT 
CATCACTCCG GTCTGATCTC CTCTCCGATC AACTTTGAGC ACATCTATCA CATGACTGTT 
AATTCTGCTG AAAAATTTCT CTCTCCTGAT TCCATAAACC CTGAATATTC CCCGTCTCTG 
CGCTCTGTCC CCGGTACACC AAGCTTTATG ACTCTGAGGA ACCCTCGGCC TCAGGAAAGT 
CGGACAGCAT TCAGTGGCTC CGTCAGTATT CCGTCTATCA CCAAATCCCG CCCTGAACCC 
GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA TCAGCAAGGT CATCTGCACA GAATGGCAGT 
GCGTTAAAGA GAGAATTCTC TGGAGGCAGC TACAGTGCAA AGCGGCAGCC CATGCCCTCC 
CCATCGGAGG GCTCGCTGTC TTCGGGAGGC ATGGACCAGG GAAGTGACGC CCCAGCACGG 
GACTATGACG GAGAGGACTC TGATTCTCCG CGGCATTCCA CGGCTTCTAA CAGTTCCAAC 
CTGAGCAGTC CTCCCAGCCC AGTGTCGCCC CGGAAGACCA AGAGCCTATC TCTGGAGAGC 
ACAGACCGCG GGAGCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832129.1
CDS	1282..6528
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKLEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_024976361.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976361.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAACAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA TTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA ACGCTCTCCA TCCTGTACTC CAGCTAGCAA AGGCAGACGT 
ACTGATCCCG TTGAGAACAC ATATGTACGG AATCCGAACG TCAAGTTTTA CATCCAGTCA 
AGGTCCACCT CTCCTTCCAC ATCTAGTGAA GCTGAGCCAG TTAAGACTGT AGACTCTACT 
CCACTTCCAG TTCATACACC AACCTTAAGG AAAAAGGGAT GCCCTGGTTC AACTGGCTTT 
CCATCGAAGC GCAAGATTCA TCAGTTTTTT GTCAAATCTT TTACTACTCC CACCAAGTGT 
CATCAGTGTA CATCTTTGAT GGTGGGTTTG ATAAGACAAG GCTGTTCTTG TGAAGTGTGT 
GGATTTTCAT GTCACATAAC TTGTGTAAAC AAAGCTCCAA CTGCTTGTCC AGTTCCTCCT 
GAACAGACAA AAGGTCCTCT GGGTATAGAT CCACAGAAAG GAATAGGAAC AGCATATGAA 
GGGCATGTCA GGATCCCTAA GCCAGCTGGA GTGAAGAAAG GATGGCAAAG AGCACTGGCT 
GTAGTCTGTG ACTTCAAGCT CTTTCTGTAC GATATTGCCG AAGGAAAGGC ATCTCAGCCC 
AGTGTTGTAG TCAGTCAAGT GATTGACATG AGGGATGAAG AATTTTCTGT GAGTTCAGTC 
TTGGCTTCTG ATGTTATTCA TGCAAGTCGA AAAGATATAC CCTGTATATT TAGAGTCACA 
GCTTCCCAGC TCTCAGCATC TAGTAACAAA TGTTCCATCC TGATGCTAGC TGATAGTGAA 
AATGAGAAGA GTAAGTGGGT GGGAGTACTG AGTGAATTGC ACAAGATTTT GAAGAAAAAC 
AAATTCAGAG ACCGCTCAGT CTATGTTCCC AAAGAGGCTT ATGACAGCAC TCTGCCCCTC 
ATTAAAACAA CCCAGGCAGC TGCAATCATA GATCATGAAA GAATAGCTTT GGGGAATGAA 
GAAGGGTTAT TTGTTGTGCA TGTCACCAAA GATGAAATTA TTAGAGTTGG TGACAATAAG 
AAGATTCATC AGATAGAACT CATTCCAAAT GATCAACTTG TTGCCGTGAT CTCAGGACGA 
AATCGACACG TCCGACTTTT TCCTATGTCA GCTCTGGACG GGCGAGAGAC TGATTTTTAT 
AAGCTGGCAG AAACTAAAGG CTGTCAAACA ATAACTTCTG GAAAAGTGCG TCATGGAGCT 
CTTACATGCT TGTGTGTGGC TATGAAAAGG CAGGTCCTCT GTTATGAACT ATTTCAGAGC 
AAGACGCGTC ACAGAAAATT TAAGGAAATT CAAGTCCCAT ACAACGTCCA GTGGATGGCA 
ATCTTCAGTG AGCAACTCTG TGTGGGATTC CAGTCTGGAT TTCTAAGATA TCCCCTGAAT 
GGAGAAGGAA GTCCATACAG TATGCTCCAT TCAAATGACA ATACACTCTC ATTTATTGCA 
CATCAACCAA TGGATGCCAT CTGTGCGGTT GAAATCTCCA GTAAAGAATA CCTGCTGTGT 
TTTAACAGCA TTGGAATATA CACTGACTGC CAGGGCCGAA GATCTAGACA ACAGGAACTG 
ATGTGGCCAG CGAATCCTTC CTCTTGTTGT TACAATGCAC CGTATCTCTC AGTGTACAGT 
GAAAATGCAG TTGACATCTT TGATGTGAAC TCCATGGAAT GGATTCAGAC TCTCCCACTC 
AAAAAGGTTC GCCCCTTAAA CAGTGAAGGA TCATTAAATC TTTTAGGCTT GGAGACGATT 
AGATTGATAT ATTTCAAAAA TAAAATGGCA GAAGGGGATG AACTGGTAGT TCCTGAAACA 
TCAGATAATA GTCGGAAGCA AATGGTTAGA AATATTAACA ATAAGAGGCG TTACTCTTTC 
AGAGTTCCGG AAGAGGAAAG AATGCAACAG AGGAGGGAGA TGTTACGAGA TCCAGAAATG 
AGAAATAAGT TAATTTCTAA CCCAACTAAT TTTAACCACA TAGCACATAT GGGTCCAGGA 
GATGGAATAC AGATCCTAAA AGACCTGCCC ATGCCGGGTT TCCCTTATCC ATCCCCTCAT 
CATCACTCCG GTCTGATCTC CTCTCCGATC AACTTTGAGC ACATCTATCA CATGACTGTT 
AATTCTGCTG AAAAATTTCT CTCTCCTGAT TCCATAAACC CTGAATATTC CCCGTCTCTG 
CGCTCTGTCC CCGGTACACC AAGCTTTATG ACTCTGAGGA ACCCTCGGCC TCAGGAAAGT 
CGGACAGCAT TCAGTGGCTC CGTCAGTATT CCGTCTATCA CCAAATCCCG CCCTGAACCC 
GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA TCAGCAAGGT CATCTGCACA GAATGGCAGT 
GCGTTAAAGA GAGAATTCTC TGGAGGCAGC TACAGTGCAA AGCGGCAGCC CATGCCCTCC 
CCATCGGAGG GCTCGCTGTC TTCGGGAGGC ATGGACCAGG GAAGTGACGC CCCAGCACGG 
GACTATGACG GAGAGGACTC TGATTCTCCG CGGCATTCCA CGGCTTCTAA CAGTTCCAAC 
CTGAGCAGTC CTCCCAGCCC AGTGTCGCCC CGGAAGACCA AGAGCCTATC TCTGGAGAGC 
ACAGACCGCG GGAGCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_024976362.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832130.1 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

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CloneID	OBa223669
Clone ID Related Accession (Same CDS sequence)	XM_024976362.1
Accession Version	XM_024976362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGTTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAAGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAGTGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGA 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCATAC 
AACGTCCAGT GGATGGCAAT CTTCAGTGAG CAACTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAATGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC AGATAATAGT CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAGTTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGC ATTCAGTGGC TCCGTCAGTA TTCCGTCTAT CACCAAATCC 
CGCCCTGAAC CCGGTCGCTC CATGAGCGCC AGCAGTGGCC TATCAGCAAG GTCATCTGCA 
CAGAATGGCA GTGCGTTAAA GAGAGAATTC TCTGGAGGCA GCTACAGTGC AAAGCGGCAG 
CCCATGCCCT CCCCATCGGA GGGCTCGCTG TCTTCGGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCAC GGGACTATGA CGGAGAGGAC TCTGATTCTC CGCGGCATTC CACGGCTTCT 
AACAGTTCCA ACCTGAGCAG TCCTCCCAGC CCAGTGTCGC CCCGGAAGAC CAAGAGCCTA 
TCTCTGGAGA GCACAGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832130.1
CDS	1282..6480
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_024976362.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976362.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGTTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAAGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAGTGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGA 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCATAC 
AACGTCCAGT GGATGGCAAT CTTCAGTGAG CAACTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAATGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC AGATAATAGT CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAGTTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGC ATTCAGTGGC TCCGTCAGTA TTCCGTCTAT CACCAAATCC 
CGCCCTGAAC CCGGTCGCTC CATGAGCGCC AGCAGTGGCC TATCAGCAAG GTCATCTGCA 
CAGAATGGCA GTGCGTTAAA GAGAGAATTC TCTGGAGGCA GCTACAGTGC AAAGCGGCAG 
CCCATGCCCT CCCCATCGGA GGGCTCGCTG TCTTCGGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCAC GGGACTATGA CGGAGAGGAC TCTGATTCTC CGCGGCATTC CACGGCTTCT 
AACAGTTCCA ACCTGAGCAG TCCTCCCAGC CCAGTGTCGC CCCGGAAGAC CAAGAGCCTA 
TCTCTGGAGA GCACAGACCG CGGGAGCTGG GACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_024976363.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832131.1 Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

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CloneID	OBa223670
Clone ID Related Accession (Same CDS sequence)	XM_024976363.1
Accession Version	XM_024976363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAACAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA TTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCTACTCCAC TTCCAGTTCA TACACCAACC 
TTAAGGAAAA AGGGATGCCC TGGTTCAACT GGCTTTCCAT CGAAGCGCAA GATTCATCAG 
TTTTTTGTCA AATCTTTTAC TACTCCCACC AAGTGTCATC AGTGTACATC TTTGATGGTG 
GGTTTGATAA GACAAGGCTG TTCTTGTGAA GTGTGTGGAT TTTCATGTCA CATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCTCTGGGT 
ATAGATCCAC AGAAAGGAAT AGGAACAGCA TATGAAGGGC ATGTCAGGAT CCCTAAGCCA 
GCTGGAGTGA AGAAAGGATG GCAAAGAGCA CTGGCTGTAG TCTGTGACTT CAAGCTCTTT 
CTGTACGATA TTGCCGAAGG AAAGGCATCT CAGCCCAGTG TTGTAGTCAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTTAGA GTCACAGCTT CCCAGCTCTC AGCATCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCTGAT AGTGAAAATG AGAAGAGTAA GTGGGTGGGA 
GTACTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ATGAAAGAAT AGCTTTGGGG AATGAAGAAG GGTTATTTGT TGTGCATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT AGAACTCATT 
CCAAATGATC AACTTGTTGC CGTGATCTCA GGACGAAATC GACACGTCCG ACTTTTTCCT 
ATGTCAGCTC TGGACGGGCG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGCTGT 
CAAACAATAA CTTCTGGAAA AGTGCGTCAT GGAGCTCTTA CATGCTTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CGCGTCACAG AAAATTTAAG 
GAAATTCAAG TCCCATACAA CGTCCAGTGG ATGGCAATCT TCAGTGAGCA ACTCTGTGTG 
GGATTCCAGT CTGGATTTCT AAGATATCCC CTGAATGGAG AAGGAAGTCC ATACAGTATG 
CTCCATTCAA ATGACAATAC ACTCTCATTT ATTGCACATC AACCAATGGA TGCCATCTGT 
GCGGTTGAAA TCTCCAGTAA AGAATACCTG CTGTGTTTTA ACAGCATTGG AATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAACTGATGT GGCCAGCGAA TCCTTCCTCT 
TGTTGTTACA ATGCACCGTA TCTCTCAGTG TACAGTGAAA ATGCAGTTGA CATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGCCC CTTAAACAGT 
GAAGGATCAT TAAATCTTTT AGGCTTGGAG ACGATTAGAT TGATATATTT CAAAAATAAA 
ATGGCAGAAG GGGATGAACT GGTAGTTCCT GAAACATCAG ATAATAGTCG GAAGCAAATG 
GTTAGAAATA TTAACAATAA GAGGCGTTAC TCTTTCAGAG TTCCGGAAGA GGAAAGAATG 
CAACAGAGGA GGGAGATGTT ACGAGATCCA GAAATGAGAA ATAAGTTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACATATGGGT CCAGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGCAT TCAGTGGCTC CGTCAGTATT 
CCGTCTATCA CCAAATCCCG CCCTGAACCC GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA 
TCAGCAAGGT CATCTGCACA GAATGGCAGT GCGTTAAAGA GAGAATTCTC TGGAGGCAGC 
TACAGTGCAA AGCGGCAGCC CATGCCCTCC CCATCGGAGG GCTCGCTGTC TTCGGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCACGG GACTATGACG GAGAGGACTC TGATTCTCCG 
CGGCATTCCA CGGCTTCTAA CAGTTCCAAC CTGAGCAGTC CTCCCAGCCC AGTGTCGCCC 
CGGAAGACCA AGAGCCTATC TCTGGAGAGC ACAGACCGCG GGAGCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832131.1
CDS	1282..6198
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKLEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_024976363.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976363.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAACAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA TTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCTACTCCAC TTCCAGTTCA TACACCAACC 
TTAAGGAAAA AGGGATGCCC TGGTTCAACT GGCTTTCCAT CGAAGCGCAA GATTCATCAG 
TTTTTTGTCA AATCTTTTAC TACTCCCACC AAGTGTCATC AGTGTACATC TTTGATGGTG 
GGTTTGATAA GACAAGGCTG TTCTTGTGAA GTGTGTGGAT TTTCATGTCA CATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCTCTGGGT 
ATAGATCCAC AGAAAGGAAT AGGAACAGCA TATGAAGGGC ATGTCAGGAT CCCTAAGCCA 
GCTGGAGTGA AGAAAGGATG GCAAAGAGCA CTGGCTGTAG TCTGTGACTT CAAGCTCTTT 
CTGTACGATA TTGCCGAAGG AAAGGCATCT CAGCCCAGTG TTGTAGTCAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTTAGA GTCACAGCTT CCCAGCTCTC AGCATCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCTGAT AGTGAAAATG AGAAGAGTAA GTGGGTGGGA 
GTACTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ATGAAAGAAT AGCTTTGGGG AATGAAGAAG GGTTATTTGT TGTGCATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT AGAACTCATT 
CCAAATGATC AACTTGTTGC CGTGATCTCA GGACGAAATC GACACGTCCG ACTTTTTCCT 
ATGTCAGCTC TGGACGGGCG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGCTGT 
CAAACAATAA CTTCTGGAAA AGTGCGTCAT GGAGCTCTTA CATGCTTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CGCGTCACAG AAAATTTAAG 
GAAATTCAAG TCCCATACAA CGTCCAGTGG ATGGCAATCT TCAGTGAGCA ACTCTGTGTG 
GGATTCCAGT CTGGATTTCT AAGATATCCC CTGAATGGAG AAGGAAGTCC ATACAGTATG 
CTCCATTCAA ATGACAATAC ACTCTCATTT ATTGCACATC AACCAATGGA TGCCATCTGT 
GCGGTTGAAA TCTCCAGTAA AGAATACCTG CTGTGTTTTA ACAGCATTGG AATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAACTGATGT GGCCAGCGAA TCCTTCCTCT 
TGTTGTTACA ATGCACCGTA TCTCTCAGTG TACAGTGAAA ATGCAGTTGA CATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGCCC CTTAAACAGT 
GAAGGATCAT TAAATCTTTT AGGCTTGGAG ACGATTAGAT TGATATATTT CAAAAATAAA 
ATGGCAGAAG GGGATGAACT GGTAGTTCCT GAAACATCAG ATAATAGTCG GAAGCAAATG 
GTTAGAAATA TTAACAATAA GAGGCGTTAC TCTTTCAGAG TTCCGGAAGA GGAAAGAATG 
CAACAGAGGA GGGAGATGTT ACGAGATCCA GAAATGAGAA ATAAGTTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACATATGGGT CCAGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGCAT TCAGTGGCTC CGTCAGTATT 
CCGTCTATCA CCAAATCCCG CCCTGAACCC GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA 
TCAGCAAGGT CATCTGCACA GAATGGCAGT GCGTTAAAGA GAGAATTCTC TGGAGGCAGC 
TACAGTGCAA AGCGGCAGCC CATGCCCTCC CCATCGGAGG GCTCGCTGTC TTCGGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCACGG GACTATGACG GAGAGGACTC TGATTCTCCG 
CGGCATTCCA CGGCTTCTAA CAGTTCCAAC CTGAGCAGTC CTCCCAGCCC AGTGTCGCCC 
CGGAAGACCA AGAGCCTATC TCTGGAGAGC ACAGACCGCG GGAGCTGGGA CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( NM_001192937.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179866.1 Bos taurus CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), mRNA.

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CloneID	OBa37050
Clone ID Related Accession (Same CDS sequence)	NM_001192937.1
Accession Version	NM_001192937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase MRCK alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03087251.1, AAFC03087250.1, AAFC03044739.1, AAFC03017398.1, AAFC03044738.1, AAFC03017401.1, AAFC02012075.1, AAFC03093465.1, AAFC03093464.1 and AAFC03093466.1. On Jul 17, 2010 this sequence version replaced XM_618596.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG AGTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCCGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCAGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCTTCGGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCA CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC TAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCAGTGTCG 
CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179866.1
CDS	263..5422
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRQELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKSDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	NM_001192937.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192937.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAAC AAGAAATAAC CAAACTAAAG AGTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCCGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCAGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCTTCGGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCA CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC TAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCAGTGTCG 
CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CDC42BPA
Entrez Gene ID	538392
Full Name	CDC42 binding protein kinase alpha
General protein information	Preferred Names CDC42 binding protein kinase alpha Names serine/threonine-protein kinase MRCK alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CDC42BPA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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