DROSHA cDNA ORF clone, Bos taurus(cattle)

The following DROSHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DROSHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa10628 XM_015468377.1
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa10628 XM_591998.9
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa10628 XM_002696390.6
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa10628 XM_015459113.2
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa10629 XM_005196187.3
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa10629 XM_005221631.4
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa10627 XM_005196186.3
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa10627 XM_010824764.2
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa10627 XM_005221630.4
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa10627 XM_010816832.3
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa10627 XM_024981526.1
Latest version!
Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa10627 XM_010816832.2 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa10628 XM_015459113.1 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa10627 XM_005221630.3 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa10628 XM_002696390.5 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa10629 XM_005221631.3 Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa10628
    Clone ID Related Accession (Same CDS sequence) XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
    Accession Version XM_002696390.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696390.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696436.1
    CDS179..4171
    Translation

    Target ORF information:

    RefSeq Version XM_002696390.5
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696390.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10628
    Clone ID Related Accession (Same CDS sequence) XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
    Accession Version XM_015459113.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314599.1
    CDS325..4317
    Translation

    Target ORF information:

    RefSeq Version XM_015459113.2
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459113.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10628
    Clone ID Related Accession (Same CDS sequence) XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
    Accession Version XM_591998.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_591998.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_591998.4
    CDS179..4171
    Translation

    Target ORF information:

    RefSeq Version XM_591998.9
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_591998.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10628
    Clone ID Related Accession (Same CDS sequence) XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
    Accession Version XM_015468377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323863.1
    CDS307..4299
    Translation

    Target ORF information:

    RefSeq Version XM_015468377.1
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10628
    Clone ID Related Accession (Same CDS sequence) XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
    Accession Version XM_015459113.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314599.1
    CDS307..4299
    Translation

    Target ORF information:

    RefSeq Version XM_015459113.1
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459113.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10628
    Clone ID Related Accession (Same CDS sequence) XM_002696390.5 , XM_015459113.2 , XM_591998.9 , XM_015468377.1 , XM_015459113.1 , XM_002696390.6
    Accession Version XM_002696390.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696390.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696436.1
    CDS197..4189
    Translation

    Target ORF information:

    RefSeq Version XM_002696390.6
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696390.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGTTCC ACCCAGGAGG ACCCAGGTGT CCCCGAGGGC GAGGGGGACA TGGAGCCAGA 
    CCCTCCTCCG CACCAGCCTT CAGGCCCCAA AATCTGAGAC TGCTTCACCC TCAGCAGCCT
    CCTGTGCAAT ACCAATACGA ACCTCCCAGC GCCCCTTCCA CCACGTTCTC CAACTCTCCG
    GCCCCCAATT TTCTGCCTCC AAGACCAGAC TTTGTACCCT TCCCTCCGCC CATGCCTCCT
    TCAGCGCAAG GCCCCCTACC CCCCTGCCCG ATCCGGCCCC CGTTCCCCAA CCACCAGATG
    AGGCCCCCCT TCCCCGTGCC CCCCTGTTTC CCTCCCATGC CGCCTCCGCT ACCCTGTCCC
    AATAACCCCC CAGTCCCCGG AGCGCCTCCT GGCCAAGGCG CCTTCCCCTT CATGATGCCG
    CCGCCATCCC TGCCGCATCC GCCGCCGCCT CCCGTCGTTC CGCAGCAGGT CAATTACCAG
    TACCCACCCG GGTACTCGCA CCACAGTTTC CCACCCCCCA ACTTCAATAG CTTCCAGAAT
    AGCCCCAGTT CCTTCCCGCC CAGTGCTAGT AACAGCAGTA GTCCTCACTT CAGGCACCTC
    CCTCCGTACC CGCTCCCCAA GGCTCCCAGT GAGAGGCGGT CCCCAGAAAG GCTAAAGCAT
    TACGACGACC ACAGGCACCG GGACCACAGT CACGGGAGGG GCGAACGGCA TCGGTCCCTG
    GAGCGCCGGG AGCGAGGCCG GAGCCCCGAC CGGAGAAGGC AGGACAGCCG CTACAGGTCC
    GATCACGAGC GGGGGCGCAC GCCCTCCCGC CACCGGAGCT ACGAGCGGAG CAGAGAGCGG
    GAACGGGAGA GACACAGGCA CCGGGACAGC CGAAGATCAC CATCTCTGGA AAGGTCCTAC
    AAAAAAGAGT ATAAGAGATC TGGAAGTCGC TCCCCAAGCA GGGAGAAGAA GAGAGGTCGC
    TGGGAGGAAG AAAAAGACAG ATGGAGTGAC ATCCCGGGCT CTGGCAAAGA GAAGAATTAT
    ACCTCAATCA AGGAAAAGGA GCCAGAGGAG GGGCTGCCTG ACAAAAATGA GGAGGAGGAA
    GAAGAACTCC TTAAGCCTGT GTGGATTCGC TGCACCCACT CAGAAAACTA CTACTCCAGT
    GACCCCATGG ATCAGGTGGG AGATTCTACT GTAGTTGGAA CAAGTAGGCT CCGTGACTTA
    TATGACAAAT TTGAGGAAGA GTTGGGGAGC AGGCAAGAGA AGGCCAAAGC TGCTAGACCC
    CCATGGGAGC CTCCCAAGAC GAAGCTAGAT GAAGATTTAG AGAGTTCCAG TGAATCCGAA
    TGCGAGTCCG ATGAGGACAG CACCTGCTCT AGCAGCTCAG ACTCTGAAGT TTTCGACGTC
    ATTGCAGAAA TCAAACGTAA AAAGGCCCAT CCTGACCGAC TTCATGATGA ACTTTGGTAC
    AACGACCCAG GCCAGATGAA TGATGGCCCA CTCTGCAAAT GCAGCGCAAA AGCGAGACGC
    ACGGGCATCA GGCACAGCAT TTATCCCGGA GAAGAGGCCA TCAAGCCCTG TCGTCCTATG
    ACCAACAATG CTGGCAGGCT TTTCCACTAT CGAATTACAG TCTCACCTCC CACAAACTTT
    TTAACTGACA GGCCAACTGT TATTGAATAT GATGATCACG AGTATATCTT TGAAGGATTT
    TCTATGTTTG CACACGCCCC CCTGACCAAT ATTCCACTGT GTAAAGTAAT TAGATTCAAC
    ATAGACTACA CGATTCATTT CATAGAAGAG ATGATGCCTG AGAATTTTTG TGTGAAAGGA
    CTTGAACTCT TTTCATTATT CCTATTCAGA GATATTTTGG AATTGTATGA CTGGAATCTC
    AAAGGTCCTT TGTTTGAAGA CAGTCCTCCC TGCTGTCCAA GATTTCATTT TATGCCACGT
    TTTGTAAGAT TTCTTCCAGA TGGAGGGAAA GAAGTACTGT CTATGCACCA GATCCTCCTC
    TACTTACTAC GGTGTAGCAA AGCCCTTGTT CCCGAAGAGG AGATTGCCAA TATGCTTCAG
    TGGGAGGAAC TTGAGTGGCA GAAATACGCG GAAGAGTGCA AAGGCATGAT TGTCACCAAC
    CCTGGAGCGA AACCAAGCTC TGTCCGCATC GATCAACTGG ATCGTGAACA GTTCAACCCT
    GATGTGATTA CTTTTCCGAT TATCGTTCAC TTTGGGATAC GCCCTGCACA GTTGAGTTAT
    GCTGGAGACC CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10629
    Clone ID Related Accession (Same CDS sequence) XM_005196187.3 , XM_005221631.3 , XM_005221631.4
    Accession Version XM_005196187.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196187.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005196244.1
    CDS221..4213
    Translation

    Target ORF information:

    RefSeq Version XM_005196187.3
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005196187.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10629
    Clone ID Related Accession (Same CDS sequence) XM_005196187.3 , XM_005221631.3 , XM_005221631.4
    Accession Version XM_005221631.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221631.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221688.1
    CDS221..4213
    Translation

    Target ORF information:

    RefSeq Version XM_005221631.3
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221631.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10629
    Clone ID Related Accession (Same CDS sequence) XM_005196187.3 , XM_005221631.3 , XM_005221631.4
    Accession Version XM_005221631.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221631.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221688.1
    CDS240..4232
    Translation

    Target ORF information:

    RefSeq Version XM_005221631.4
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221631.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTACCA GAAACTGTGG AAGAGTTATG TTAAGCTTCG CCACCTCCTA
    GCAAATAGTC CAAAAGTCAA ACAGACTGAC AAGCAGAAGC TGGCACAGAG GGAGGAAGCG
    CTCCAAAAAA TACGACAGAA GAATACCATG AGGCGAGAAG TAACGGTGGA GCTGAGTAGC
    CAAGGCTTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC AGCATGCCAT GATGCTGCCA
    GTTCTGACCC ATCATATTCG CTACCACCAG TGCCTAATGC ATCTGGACAA GTTGATAGGA
    TATACTTTCC AAGATCGTTG TCTGTTACAG CTGGCCATGA CCCACCCAAG TCATCACTTA
    AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCTTTGT CTAACTGTGG AATTCGGCAG
    CCCAAATATG GAGATAGAAA AGTTCATCAC ATGCACATGC GGAAAAAAGG AATTAACACC
    TTAATAAATA TTATGTCACG CCTTGGCCAA GATGACCCAA CTCCCTCAAG GATTAATCAC
    AACGAACGGT TGGAGTTCCT GGGTGATGCT GTCGTTGAAT TTCTGACCAG TGTCCATTTG
    TACTATCTGT TTCCCAGTCT GGAAGAGGGG GGATTAGCAA CCTATCGGAC TGCCATCGTT
    CAGAACCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC TGGATCGGTT TATGCTTTAT
    GCTCATGGAC CTGACCTTTG TAGAGAATCA GACCTTCGGC ATGCAATGGC TAATTGTTTT
    GAAGCATTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG AGGAAGCCAA GCAGTTATTT
    GGACGCTTAC TCTTTAATGA TCCGGACCTG CGCGAAGTCT GGCTCAATTA TCCTCTCCAC
    CCACTCCAGC TTCAAGAGCC AAATACTGAT CGACAACTTA TTGAGACTTC TCCAGTTCTA
    CAGAAACTTA CTGAATTTGA AGATGCAATT GGAGTAATTT TTACACATGT TCGACTTTTG
    GCAAGAGCAT TCACATTGAG AACTGTGGGA TTTAACCATC TGACCCTAGG CCACAATCAG
    AGGATGGAAT TCCTTGGTGA CTCCATAATG CAGCTGGTAG CAACGGAGTA CTTATTCATT
    CATTTTCCAG ATCATCACGA AGGACACTTA ACTCTGTTGC GAAGCTCTTT GGTGAATAAC
    AGAACTCAGG CCAAGGTGGC GGAGGAATTG GGCATGCAGG AATACGCCAT CACCAATGAC
    AAGACCAAAA GACCTGTCGC CCTGAGAACC AAGACCTTGG CTGACCTTTT GGAATCATTT
    ATTGCAGCAC TGTACATTGA TAAAGACTTG GAATACGTTC ATACTTTCAT GAATGTTTGT
    TTCTTTCCAC GATTAAAAGA ATTCATTTTG AATCAGGATT GGAACGACCC CAAGTCCCAG
    CTTCAGCAGT GCTGCCTGAC TCTTAGGACA GAAGGAAAAG AACCAGACAT TCCGCTATAC
    AAGACTCTGC AGACAGTGGG GCCATCCCAC GCGAGGACCT ACACTGTGGC TGTCTACTTC
    AAGGGAGAAA GAATTGGCTG TGGGAAAGGA CCAAGTATTC AGCAAGCAGA AATGGGAGCA
    GCAATGGATG CACTTGAAAA ATATAACTTT CCCCAGATGG CCCATCAGAA ACGGTTCATT
    GAACGGAAAT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG AAAGAGAGCA TCAAGAGAAG
    GAGCCAGATG AGACCGAAGA CATAAAGAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10627
    Clone ID Related Accession (Same CDS sequence) XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
    Accession Version XM_010816832.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815134.1
    CDS349..4365
    Translation

    Target ORF information:

    RefSeq Version XM_010816832.2
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816832.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10627
    Clone ID Related Accession (Same CDS sequence) XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
    Accession Version XM_024981526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837294.1
    CDS77..4093
    Translation

    Target ORF information:

    RefSeq Version XM_024981526.1
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10627
    Clone ID Related Accession (Same CDS sequence) XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
    Accession Version XM_005196186.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196186.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005196243.1
    CDS221..4237
    Translation

    Target ORF information:

    RefSeq Version XM_005196186.3
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005196186.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10627
    Clone ID Related Accession (Same CDS sequence) XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
    Accession Version XM_010816832.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815134.1
    CDS367..4383
    Translation

    Target ORF information:

    RefSeq Version XM_010816832.3
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816832.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10627
    Clone ID Related Accession (Same CDS sequence) XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
    Accession Version XM_005221630.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221630.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221687.1
    CDS221..4237
    Translation

    Target ORF information:

    RefSeq Version XM_005221630.3
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221630.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10627
    Clone ID Related Accession (Same CDS sequence) XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
    Accession Version XM_010824764.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010823066.1
    CDS349..4365
    Translation

    Target ORF information:

    RefSeq Version XM_010824764.2
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824764.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10627
    Clone ID Related Accession (Same CDS sequence) XM_010816832.2 , XM_024981526.1 , XM_005196186.3 , XM_010816832.3 , XM_005221630.3 , XM_010824764.2 , XM_005221630.4
    Accession Version XM_005221630.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ribonuclease 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221630.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221687.1
    CDS239..4255
    Translation

    Target ORF information:

    RefSeq Version XM_005221630.4
    Organism Bos taurus(cattle)
    Definition Bos taurus drosha ribonuclease III (DROSHA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221630.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAAGGCA GTGCATGTCA CAGAATGTCG TTCCACCCAG GAGGACCCAG GTGTCCCCGA 
    GGGCGAGGGG GACATGGAGC CAGACCCTCC TCCGCACCAG CCTTCAGGCC CCAAAATCTG
    AGACTGCTTC ACCCTCAGCA GCCTCCTGTG CAATACCAAT ACGAACCTCC CAGCGCCCCT
    TCCACCACGT TCTCCAACTC TCCGGCCCCC AATTTTCTGC CTCCAAGACC AGACTTTGTA
    CCCTTCCCTC CGCCCATGCC TCCTTCAGCG CAAGGCCCCC TACCCCCCTG CCCGATCCGG
    CCCCCGTTCC CCAACCACCA GATGAGGCCC CCCTTCCCCG TGCCCCCCTG TTTCCCTCCC
    ATGCCGCCTC CGCTACCCTG TCCCAATAAC CCCCCAGTCC CCGGAGCGCC TCCTGGCCAA
    GGCGCCTTCC CCTTCATGAT GCCGCCGCCA TCCCTGCCGC ATCCGCCGCC GCCTCCCGTC
    GTTCCGCAGC AGGTCAATTA CCAGTACCCA CCCGGGTACT CGCACCACAG TTTCCCACCC
    CCCAACTTCA ATAGCTTCCA GAATAGCCCC AGTTCCTTCC CGCCCAGTGC TAGTAACAGC
    AGTAGTCCTC ACTTCAGGCA CCTCCCTCCG TACCCGCTCC CCAAGGCTCC CAGTGAGAGG
    CGGTCCCCAG AAAGGCTAAA GCATTACGAC GACCACAGGC ACCGGGACCA CAGTCACGGG
    AGGGGCGAAC GGCATCGGTC CCTGGAGCGC CGGGAGCGAG GCCGGAGCCC CGACCGGAGA
    AGGCAGGACA GCCGCTACAG GTCCGATCAC GAGCGGGGGC GCACGCCCTC CCGCCACCGG
    AGCTACGAGC GGAGCAGAGA GCGGGAACGG GAGAGACACA GGCACCGGGA CAGCCGAAGA
    TCACCATCTC TGGAAAGGTC CTACAAAAAA GAGTATAAGA GATCTGGAAG TCGCTCCCCA
    AGCAGGGAGA AGAAGAGAGG TCGCTGGGAG GAAGAAAAAG ACAGATGGAG TGACATCCCG
    GGCTCTGGCA AAGAGAAGAA TTATACCTCA ATCAAGGAAA AGGAGCCAGA GGAGGGGCTG
    CCTGACAAAA ATGAGGAGGA GGAAGAAGAA CTCCTTAAGC CTGTGTGGAT TCGCTGCACC
    CACTCAGAAA ACTACTACTC CAGTGACCCC ATGGATCAGG TGGGAGATTC TACTGTAGTT
    GGAACAAGTA GGCTCCGTGA CTTATATGAC AAATTTGAGG AAGAGTTGGG GAGCAGGCAA
    GAGAAGGCCA AAGCTGCTAG ACCCCCATGG GAGCCTCCCA AGACGAAGCT AGATGAAGAT
    TTAGAGAGTT CCAGTGAATC CGAATGCGAG TCCGATGAGG ACAGCACCTG CTCTAGCAGC
    TCAGACTCTG AAGTTTTCGA CGTCATTGCA GAAATCAAAC GTAAAAAGGC CCATCCTGAC
    CGACTTCATG ATGAACTTTG GTACAACGAC CCAGGCCAGA TGAATGATGG CCCACTCTGC
    AAATGCAGCG CAAAAGCGAG ACGCACGGGC ATCAGGCACA GCATTTATCC CGGAGAAGAG
    GCCATCAAGC CCTGTCGTCC TATGACCAAC AATGCTGGCA GGCTTTTCCA CTATCGAATT
    ACAGTCTCAC CTCCCACAAA CTTTTTAACT GACAGGCCAA CTGTTATTGA ATATGATGAT
    CACGAGTATA TCTTTGAAGG ATTTTCTATG TTTGCACACG CCCCCCTGAC CAATATTCCA
    CTGTGTAAAG TAATTAGATT CAACATAGAC TACACGATTC ATTTCATAGA AGAGATGATG
    CCTGAGAATT TTTGTGTGAA AGGACTTGAA CTCTTTTCAT TATTCCTATT CAGAGATATT
    TTGGAATTGT ATGACTGGAA TCTCAAAGGT CCTTTGTTTG AAGACAGTCC TCCCTGCTGT
    CCAAGATTTC ATTTTATGCC ACGTTTTGTA AGATTTCTTC CAGATGGAGG GAAAGAAGTA
    CTGTCTATGC ACCAGATCCT CCTCTACTTA CTACGGTGTA GCAAAGCCCT TGTTCCCGAA
    GAGGAGATTG CCAATATGCT TCAGTGGGAG GAACTTGAGT GGCAGAAATA CGCGGAAGAG
    TGCAAAGGCA TGATTGTCAC CAACCCTGGA GCGAAACCAA GCTCTGTCCG CATCGATCAA
    CTGGATCGTG AACAGTTCAA CCCTGATGTG ATTACTTTTC CGATTATCGT TCACTTTGGG
    ATACGCCCTG CACAGTTGAG TTATGCTGGA GACCCACAGT ACCAGAAACT GTGGAAGAGT
    TATGTTAAGC TTCGCCACCT CCTAGCAAAT AGTCCAAAAG TCAAACAGAC TGACAAGCAG
    AAGCTGGCAC AGAGGGAGGA AGCGCTCCAA AAAATACGAC AGAAGAATAC CATGAGGCGA
    GAAGTAACGG TGGAGCTGAG TAGCCAAGGC TTCTGGAAAA CTGGCATCCG TTCTGATGTC
    TGTCAGCATG CCATGATGCT GCCAGTTCTG ACCCATCATA TTCGCTACCA CCAGTGCCTA
    ATGCATCTGG ACAAGTTGAT AGGATATACT TTCCAAGATC GTTGTCTGTT ACAGCTGGCC
    ATGACCCACC CAAGTCATCA CTTAAATTTT GGAATGAATC CTGATCATGC CAGGAATTCT
    TTGTCTAACT GTGGAATTCG GCAGCCCAAA TATGGAGATA GAAAAGTTCA TCACATGCAC
    ATGCGGAAAA AAGGAATTAA CACCTTAATA AATATTATGT CACGCCTTGG CCAAGATGAC
    CCAACTCCCT CAAGGATTAA TCACAACGAA CGGTTGGAGT TCCTGGGTGA TGCTGTCGTT
    GAATTTCTGA CCAGTGTCCA TTTGTACTAT CTGTTTCCCA GTCTGGAAGA GGGGGGATTA
    GCAACCTATC GGACTGCCAT CGTTCAGAAC CAGCACCTTG CCATGCTAGC AAAGAAACTT
    GAACTGGATC GGTTTATGCT TTATGCTCAT GGACCTGACC TTTGTAGAGA ATCAGACCTT
    CGGCATGCAA TGGCTAATTG TTTTGAAGCA TTAATAGGAG CTGTTTACTT GGAGGGAAGC
    CTGGAGGAAG CCAAGCAGTT ATTTGGACGC TTACTCTTTA ATGATCCGGA CCTGCGCGAA
    GTCTGGCTCA ATTATCCTCT CCACCCACTC CAGCTTCAAG AGCCAAATAC TGATCGACAA
    CTTATTGAGA CTTCTCCAGT TCTACAGAAA CTTACTGAAT TTGAAGATGC AATTGGAGTA
    ATTTTTACAC ATGTTCGACT TTTGGCAAGA GCATTCACAT TGAGAACTGT GGGATTTAAC
    CATCTGACCC TAGGCCACAA TCAGAGGATG GAATTCCTTG GTGACTCCAT AATGCAGCTG
    GTAGCAACGG AGTACTTATT CATTCATTTT CCAGATCATC ACGAAGGACA CTTAACTCTG
    TTGCGAAGCT CTTTGGTGAA TAACAGAACT CAGGCCAAGG TGGCGGAGGA ATTGGGCATG
    CAGGAATACG CCATCACCAA TGACAAGACC AAAAGACCTG TCGCCCTGAG AACCAAGACC
    TTGGCTGACC TTTTGGAATC ATTTATTGCA GCACTGTACA TTGATAAAGA CTTGGAATAC
    GTTCATACTT TCATGAATGT TTGTTTCTTT CCACGATTAA AAGAATTCAT TTTGAATCAG
    GATTGGAACG ACCCCAAGTC CCAGCTTCAG CAGTGCTGCC TGACTCTTAG GACAGAAGGA
    AAAGAACCAG ACATTCCGCT ATACAAGACT CTGCAGACAG TGGGGCCATC CCACGCGAGG
    ACCTACACTG TGGCTGTCTA CTTCAAGGGA GAAAGAATTG GCTGTGGGAA AGGACCAAGT
    ATTCAGCAAG CAGAAATGGG AGCAGCAATG GATGCACTTG AAAAATATAA CTTTCCCCAG
    ATGGCCCATC AGAAACGGTT CATTGAACGG AAATACAGAC AAGAGTTAAA AGAAATGAGG
    TGGGAAAGAG AGCATCAAGA GAAGGAGCCA GATGAGACCG AAGACATAAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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