MAP3K6 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	MAP3K6
Entrez Gene ID	614598
Full Name	mitogen-activated protein kinase kinase kinase 6
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 6 Names mitogen-activated protein kinase kinase kinase 6
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001193236.3	NP_001180165.3	mitogen-activated protein kinase kinase kinase 6
NM_001193236.1	NP_001180165.1	mitogen-activated protein kinase kinase kinase 6
XM_005203237.3	XP_005203294.1	mitogen-activated protein kinase kinase kinase 6 isoform X1
XM_005203237.4	XP_005203294.2	mitogen-activated protein kinase kinase kinase 6 isoform X1
XM_010802602.3	XP_010800904.2	mitogen-activated protein kinase kinase kinase 6 isoform X2
XM_010802602.2	XP_010800904.1	mitogen-activated protein kinase kinase kinase 6 isoform X2
NM_001193236.2	NP_001180165.2	mitogen-activated protein kinase kinase kinase 6

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MAP3K6	XP_005617983.1
Mus musculus (house mouse)	Map3k6	NP_057902.5
Homo sapiens (human)	MAP3K6	NP_004663.3
Gallus gallus (chicken)	MAP3K6	XP_003642613.2
Rattus norvegicus (Norway rat)	Map3k6	NP_001101379.1
Pan troglodytes (chimpanzee)	MAP3K6	XP_513244.2
	MAP3K6	XP_001111161.1
Bos taurus (cattle)	MAP3K6	NP_001180165.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAP3K6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa227552	XM_005203237.4 Latest version!	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa227553	XM_010802602.3 Latest version!	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa216284	NM_001193236.2 Latest version!	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa40511	NM_001193236.3 Latest version!	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa16997	XM_005203237.3 Latest version!	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa55461	XM_010802602.2 Latest version!	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OBa40511	NM_001193236.1	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K6 ( XM_005203237.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203294.2 Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa227552
Clone ID Related Accession (Same CDS sequence)	XM_005203237.4
Accession Version	XM_005203237.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203237.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG TGAAGTCGCC GGACGTTCCC TCCAGCTGGA CCTTCCAGGT GGCCTCCATC 
AGCGGCGTCA GCGTCTCCAA GCGGGACGAG CGCTGCTGCT TCCTCTACGC GCCTCCTCCG 
GCTCAGGACG TCCAGCTGTG CTTCCCCAGC GCCGGGCACT GCCAGTGGTT CTGCGGCCTG 
ATCCAGGCCT TGGTGAAGAG TCCAGACTCC GGGGCGCCCG CAGAGGAGAC GGAGGGCGCT 
GGGGAGGTGT TGGAGTTTGA TTACGAGTAC ACGGAGTCGG GTGAGCGGCT GGTGCTGGGC 
AAGGGCACGT ACGGGGTGGT GTACGCTGGC CGCGATCGAC ACACTCGCGT GCGCATCGCC 
ATCAAGGAGA TCCCGGAGCG GGACAGCAGG TTCTCTCAGC CGCTGCATGA AGAGATCGCT 
CTGCACAAAC GCCTACGCCA CAAGAACATT GTGCGCTATC TGGGCTCCGT CAGCCAGGGC 
GGCTACCTCA AGATTTTCAT GGAGGAAGTG CCTGGAGGCA GCCTGTCTTC CTTGCTGCGA 
TCGGTGTGGG GACCCCTGCA GGACAACGAG AGCACCATCA GTTTCTACAC CCGCCAGATC 
CTGCAGGGAC TCAGCTACCT GCACGACAAC CACATTGTGC ATCGAGATAT CAAGGGGGAC 
AACGTGCTGA TCAACACCTT CAGCGGGTTG CTCAAGATTT CTGACTTTGG CACCTCCAAG 
CGACTAGCAG GCATCACACC CTGCACTGAG ACCTTCACAG GGACCCTACA ATATATGGCC 
CCAGAAATCA TTGACCAGGG CCCACGAGGG TATGGGAAGG CAGCAGACAT CTGGTCACTG 
GGCTGCACAG TCATCGAGAT GGCCACAGGT CACCCACCCT TCCACGAGCT AGGGAGCCCG 
CAGGCTGCCA TGTTTCAGGT GGGCATGTAC AAGGTGCATC CGCCAATGCC CGGTTCTCTG 
TCAGCTGAGG CTCAAGCCTT CCTCCTCCGA ACTTTTGAGC CCGACCCCCG TCTCCGAGCC 
AGTGCTCAAG CACTGCTGGC AGACCCCTTC CTGCAGCCCG GGAAGAGGAG CCGCAGCCCG 
GGCTCCCCCA GACAGGCTCT AAGGTCCTCA GAGGCGCCAT CCGCAAGCCT TGCTCCTTCA 
GCAGACTCCA CCTCCCAGTC CCAGACATTC CCGCGCCCTC AGGCACCCTC TCAGCACCCT 
CCCAGTCCCC CCAAGCGCTG CCTCAGTTAT GGGGACACCA GCCAGCTCCG GGTGCCCGAG 
GAGCCTGGGG CTGACGAGCC CGCGTCCCCT GAGGAGAGTT CGGGGCTGAG CCTCTTGCAT 
CAAGAGAGTA GGCGCCGGGC CACGCTGGCC ACGGTTCTGG AGCAGGAGCT GCCGGCGCTG 
GCGGAGAGCC TGCGCCTGGA GCAGGAACAG GGTCCCCGTC TGGAGAGGAG TCACGTGGAA 
CAGCTACTGC TCTGCCTCAG GGCGCACATC CATACTCCCA ACCGTCGGCA GCTGGCCCAG 
GAGCTGCGGG GACTCCAAGG GCAGCTTCGG GCCCAGGGCC TTGAGCCCGA GCTTCTGCAA 
GGACCGCTCT TCGCCTTCCC GGATGAGGTG AAGCAGGTCC TCCGCAGGCG CCAGATCCGC 
CCACACTGGA TGTTCGTGCT GGACTCGCTG CTCAGCCGTG CTGTGCGGGC AGCCCTGGCA 
GTGCTGGCCC CAGAGGTGGA GGAGGAGGCG GTCCTACCGA AGTCGGAAGA GGCCAGTAAT 
GCAGAGGAGT CCGAGCCCAA GCAGCAGGAG ACCCCAGCCG AGCCGAGCCC TCTCCTGGGG 
GAACCGGAGC AGGGTACCCC TTCTCTGATG GTGCAGCTGG GCCTCTTGCG AGCAGAGACC 
GATAGGCTTC GAGATGTTCT GGCTGAGAAG GAAAGGGAAT GCCAGGCCTT GGTGCAACTA 
GCCCTCCAGC GGGTCAGTGG GGAGGCCAGG ACCTGTGCCC TGGCTTCAGA GCCCCCAGCG 
GCTCTCGCGG TGGACCAGGG CCTGGTGCAG TGGTTACAGG AACTGAATGT GGATTCAGGC 
ACCATCCAGA CGCTACTGAA CCACAGCTTC ACCCTCCATG CCCTACTGAC CTGTGCCACT 
AGAGATGACC TCATCTACAC AGGCATCAGG AGGCTGAATG AGGACATCCG AGGCAGGAGG 
CCAGACCCAA GGATGGATGA ATGGAGGAAA CACAAGAGGC TTCTGACATA CCTGCCCCAG 
GACCCAGCAG CAACTGGAAG AGGCCAGGTG GGAGGGCGTC AGGGTAGGGG CCAGGCCTCT 
AGCCCCAGTG GGGAGAACAA ATCACAGATG TGCCTCAAGC TCATGAGGGC CACAGGCAAA 
GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203294.2
CDS	1678..5706
Translation	MAGPSPRSGALERAGSCWQDPLAEALSRGRPLAASPGRGCARSRPLSVVYVLTREPLPEVEPETGAEAEPLPLRCLREACAQLPGPRPRPQLHSLPFGTLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDIFQKNSDCIGSYTLIPYVVTATGRVLCGDAGLLRGLADGLLQAGVGTEALLTPLVGRLAHLLEATPTDSCGYFRETIRQDIRRARERFSGLQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIDLVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVQVEGPVAPDLYCMCGRIYKDMFFSSRFQDAGHREQAYHWYRKAFDIEPSLHSGINAAVLLIADGQRFEDSEELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDLTQVALAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTLELPGEAPHGAHFWLHFLLQSCQPLKTACPQGDQCLVLVLEMNKVLLPARLEVQGTNPVSAVTLSLLEPEKQVKSPDVPSSWTFQVASISGVSVSKRDERCCFLYAPPPAQDVQLCFPSAGHCQWFCGLIQALVKSPDSGAPAEETEGAGEVLEFDYEYTESGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSVSQGGYLKIFMEEVPGGSLSSLLRSVWGPLQDNESTISFYTRQILQGLSYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGHPPFHELGSPQAAMFQVGMYKVHPPMPGSLSAEAQAFLLRTFEPDPRLRASAQALLADPFLQPGKRSRSPGSPRQALRSSEAPSASLAPSADSTSQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPGADEPASPEESSGLSLLHQESRRRATLATVLEQELPALAESLRLEQEQGPRLERSHVEQLLLCLRAHIHTPNRRQLAQELRGLQGQLRAQGLEPELLQGPLFAFPDEVKQVLRRRQIRPHWMFVLDSLLSRAVRAALAVLAPEVEEEAVLPKSEEASNAEESEPKQQETPAEPSPLLGEPEQGTPSLMVQLGLLRAETDRLRDVLAEKERECQALVQLALQRVSGEARTCALASEPPAALAVDQGLVQWLQELNVDSGTIQTLLNHSFTLHALLTCATRDDLIYTGIRRLNEDIRGRRPDPRMDEWRKHKRLLTYLPQDPAATGRGQVGGRQGRGQASSPSGENKSQMCLKLMRATGKDY

Target ORF information:

RefSeq Version	XM_005203237.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203237.4

ORF Insert Sequence:

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG TGAAGTCGCC GGACGTTCCC TCCAGCTGGA CCTTCCAGGT GGCCTCCATC 
AGCGGCGTCA GCGTCTCCAA GCGGGACGAG CGCTGCTGCT TCCTCTACGC GCCTCCTCCG 
GCTCAGGACG TCCAGCTGTG CTTCCCCAGC GCCGGGCACT GCCAGTGGTT CTGCGGCCTG 
ATCCAGGCCT TGGTGAAGAG TCCAGACTCC GGGGCGCCCG CAGAGGAGAC GGAGGGCGCT 
GGGGAGGTGT TGGAGTTTGA TTACGAGTAC ACGGAGTCGG GTGAGCGGCT GGTGCTGGGC 
AAGGGCACGT ACGGGGTGGT GTACGCTGGC CGCGATCGAC ACACTCGCGT GCGCATCGCC 
ATCAAGGAGA TCCCGGAGCG GGACAGCAGG TTCTCTCAGC CGCTGCATGA AGAGATCGCT 
CTGCACAAAC GCCTACGCCA CAAGAACATT GTGCGCTATC TGGGCTCCGT CAGCCAGGGC 
GGCTACCTCA AGATTTTCAT GGAGGAAGTG CCTGGAGGCA GCCTGTCTTC CTTGCTGCGA 
TCGGTGTGGG GACCCCTGCA GGACAACGAG AGCACCATCA GTTTCTACAC CCGCCAGATC 
CTGCAGGGAC TCAGCTACCT GCACGACAAC CACATTGTGC ATCGAGATAT CAAGGGGGAC 
AACGTGCTGA TCAACACCTT CAGCGGGTTG CTCAAGATTT CTGACTTTGG CACCTCCAAG 
CGACTAGCAG GCATCACACC CTGCACTGAG ACCTTCACAG GGACCCTACA ATATATGGCC 
CCAGAAATCA TTGACCAGGG CCCACGAGGG TATGGGAAGG CAGCAGACAT CTGGTCACTG 
GGCTGCACAG TCATCGAGAT GGCCACAGGT CACCCACCCT TCCACGAGCT AGGGAGCCCG 
CAGGCTGCCA TGTTTCAGGT GGGCATGTAC AAGGTGCATC CGCCAATGCC CGGTTCTCTG 
TCAGCTGAGG CTCAAGCCTT CCTCCTCCGA ACTTTTGAGC CCGACCCCCG TCTCCGAGCC 
AGTGCTCAAG CACTGCTGGC AGACCCCTTC CTGCAGCCCG GGAAGAGGAG CCGCAGCCCG 
GGCTCCCCCA GACAGGCTCT AAGGTCCTCA GAGGCGCCAT CCGCAAGCCT TGCTCCTTCA 
GCAGACTCCA CCTCCCAGTC CCAGACATTC CCGCGCCCTC AGGCACCCTC TCAGCACCCT 
CCCAGTCCCC CCAAGCGCTG CCTCAGTTAT GGGGACACCA GCCAGCTCCG GGTGCCCGAG 
GAGCCTGGGG CTGACGAGCC CGCGTCCCCT GAGGAGAGTT CGGGGCTGAG CCTCTTGCAT 
CAAGAGAGTA GGCGCCGGGC CACGCTGGCC ACGGTTCTGG AGCAGGAGCT GCCGGCGCTG 
GCGGAGAGCC TGCGCCTGGA GCAGGAACAG GGTCCCCGTC TGGAGAGGAG TCACGTGGAA 
CAGCTACTGC TCTGCCTCAG GGCGCACATC CATACTCCCA ACCGTCGGCA GCTGGCCCAG 
GAGCTGCGGG GACTCCAAGG GCAGCTTCGG GCCCAGGGCC TTGAGCCCGA GCTTCTGCAA 
GGACCGCTCT TCGCCTTCCC GGATGAGGTG AAGCAGGTCC TCCGCAGGCG CCAGATCCGC 
CCACACTGGA TGTTCGTGCT GGACTCGCTG CTCAGCCGTG CTGTGCGGGC AGCCCTGGCA 
GTGCTGGCCC CAGAGGTGGA GGAGGAGGCG GTCCTACCGA AGTCGGAAGA GGCCAGTAAT 
GCAGAGGAGT CCGAGCCCAA GCAGCAGGAG ACCCCAGCCG AGCCGAGCCC TCTCCTGGGG 
GAACCGGAGC AGGGTACCCC TTCTCTGATG GTGCAGCTGG GCCTCTTGCG AGCAGAGACC 
GATAGGCTTC GAGATGTTCT GGCTGAGAAG GAAAGGGAAT GCCAGGCCTT GGTGCAACTA 
GCCCTCCAGC GGGTCAGTGG GGAGGCCAGG ACCTGTGCCC TGGCTTCAGA GCCCCCAGCG 
GCTCTCGCGG TGGACCAGGG CCTGGTGCAG TGGTTACAGG AACTGAATGT GGATTCAGGC 
ACCATCCAGA CGCTACTGAA CCACAGCTTC ACCCTCCATG CCCTACTGAC CTGTGCCACT 
AGAGATGACC TCATCTACAC AGGCATCAGG AGGCTGAATG AGGACATCCG AGGCAGGAGG 
CCAGACCCAA GGATGGATGA ATGGAGGAAA CACAAGAGGC TTCTGACATA CCTGCCCCAG 
GACCCAGCAG CAACTGGAAG AGGCCAGGTG GGAGGGCGTC AGGGTAGGGG CCAGGCCTCT 
AGCCCCAGTG GGGAGAACAA ATCACAGATG TGCCTCAAGC TCATGAGGGC CACAGGCAAA 
GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K6 ( XM_010802602.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800904.2 Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa227553
Clone ID Related Accession (Same CDS sequence)	XM_010802602.3
Accession Version	XM_010802602.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802602.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG TGAAGTCGCC GGACGTTCCC TCCAGCTGGA CCTTCCAGGT GGCCTCCATC 
AGCGGCGTCA GCGTCTCCAA GCGGGACGAG CGCTGCTGCT TCCTCTACGC GCCTCCTCCG 
GCTCAGGACG TCCAGCTGTG CTTCCCCAGC GCCGGGCACT GCCAGTGGTT CTGCGGCCTG 
ATCCAGGCCT TGGTGAAGAG TCCAGACTCC GGGGCGCCCG CAGAGGAGAC GGAGGGCGCT 
GGGGAGGTGT TGGAGTTTGA TTACGAGTAC ACGGAGTCGG GTGAGCGGCT GGTGCTGGGC 
AAGGGCACGT ACGGGGTGGT GTACGCTGGC CGCGATCGAC ACACTCGCGT GCGCATCGCC 
ATCAAGGAGA TCCCGGAGCG GGACAGCAGG TTCTCTCAGC CGCTGCATGA AGAGATCGCT 
CTGCACAAAC GCCTACGCCA CAAGAACATT GTGCGCTATC TGGGCTCCGT CAGCCAGGGC 
GGCTACCTCA AGATTTTCAT GGAGGAAGTG CCTGGAGGCA GCCTGTCTTC CTTGCTGCGA 
TCGGTGTGGG GACCCCTGCA GGACAACGAG AGCACCATCA GTTTCTACAC CCGCCAGATC 
CTGCAGGGAC TCAGCTACCT GCACGACAAC CACATTGTGC ATCGAGATAT CAAGGGGGAC 
AACGTGCTGA TCAACACCTT CAGCGGGTTG CTCAAGATTT CTGACTTTGG CACCTCCAAG 
CGACTAGCAG GCATCACACC CTGCACTGAG ACCTTCACAG GGACCCTACA ATATATGGCC 
CCAGAAATCA TTGACCAGGG CCCACGAGGG TATGGGAAGG CAGCAGACAT CTGGTCACTG 
GGCTGCACAG TCATCGAGAT GGCCACAGGT CACCCACCCT TCCACGAGCT AGGGAGCCCG 
CAGGCTGCCA TGTTTCAGGT GGGCATGTAC AAGGTGCATC CGCCAATGCC CGGTTCTCTG 
TCAGCTGAGG CTCAAGCCTT CCTCCTCCGA ACTTTTGAGC CCGACCCCCG TCTCCGAGCC 
AGTGCTCAAG CACTGCTGGC AGACCCCTTC CTGCAGCCCG GGAAGAGGAG CCGCAGCCCG 
GGCTCCCCCA GACAGGCTCT AAGGTCCTCA GAGGCGCCAT CCGCAAGCCT TGCTCCTTCA 
GCAGACTCCA CCTCCCAGTC CCAGACATTC CCGCGCCCTC AGGCACCCTC TCAGCACCCT 
CCCAGTCCCC CCAAGCGCTG CCTCAGTTAT GGGGACACCA GCCAGCTCCG GGTGCCCGAG 
GAGCCTGGGG CTGACGAGCC CGCGTCCCCT GAGGAGAGTT CGGGGCTGAG CCTCTTGCAT 
CAAGAGAGTA GGCGCCGGGC CACGCTGGCC ACGGTTCTGG AGCAGGAGCT GCCGGCGCTG 
GCGGAGAGCC TGCGCCTGGA GCAGGAACAG GGTCCCCGTC TGGAGAGGAG TCACGTGGAA 
CAGCTACTGC TCTGCCTCAG GGCGCACATC CATACTCCCA ACCGTCGGCA GCTGGCCCAG 
GAGCTGCGGG GACTCCAAGG GCAGCTTCGG GCCCAGGGCC TTGAGCCCGA GCTTCTGCAA 
GGACCGCTCT TCGCCTTCCC GGATGAGGTG AAGCAGGTCC TCCGCAGGCG CCAGATCCGC 
CCACACTGGA TGTTCGTGCT GGACTCGCTG CTCAGCCGTG CTGTGCGGGC AGCCCTGGCA 
GTGCTGGCCC CAGAGGTGGA GGAGGAGGCG GTCCTACCGA AGTCGGAAGA GGCCAGTAAT 
GCAGAGGAGT CCGAGCCCAA GCAGCAGGAG ACCCCAGCCG AGCCGAGCCC TCTCCTGGGG 
GAACCGGAGC AGGGTACCCC TTCTCTGATG GTGCAGCTGG GCCTCTTGCG AGCAGAGACC 
GATAGGCTTC GAGATGTTCT GGCTGAGAAG GAAAGGGAAT GCCAGGCCTT GGTGCAACTA 
GCCCTCCAGC GGGTCAGTGG GGAGGCCAGG ACCTGTGCCC TGGCTTCAGA GCCCCCAGCG 
GCTCTCGCGG TGGACCAGGG CCTGGTGCAG TGGTTACAGG AACTGAATGT GGATTCAGGC 
ACCATCCAGA CGCTACTGAA CCACAGCTTC ACCCTCCATG CCCTACTGAC CTGTGCCACT 
AGAGATGACC TCATCTACAC AGGCATCAGG GGGGGGATGA TATGCCGCAT CTGGAGAGCC 
ATCTTGGCAC AGCGAACAAG ATCCACGCCA GTTACCCCTG GCCCCCAGGA GGCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800904.2
CDS	1677..5576
Translation	MAGPSPRSGALERAGSCWQDPLAEALSRGRPLAASPGRGCARSRPLSVVYVLTREPLPEVEPETGAEAEPLPLRCLREACAQLPGPRPRPQLHSLPFGTLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDIFQKNSDCIGSYTLIPYVVTATGRVLCGDAGLLRGLADGLLQAGVGTEALLTPLVGRLAHLLEATPTDSCGYFRETIRQDIRRARERFSGLQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIDLVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVQVEGPVAPDLYCMCGRIYKDMFFSSRFQDAGHREQAYHWYRKAFDIEPSLHSGINAAVLLIADGQRFEDSEELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDLTQVALAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTLELPGEAPHGAHFWLHFLLQSCQPLKTACPQGDQCLVLVLEMNKVLLPARLEVQGTNPVSAVTLSLLEPEKQVKSPDVPSSWTFQVASISGVSVSKRDERCCFLYAPPPAQDVQLCFPSAGHCQWFCGLIQALVKSPDSGAPAEETEGAGEVLEFDYEYTESGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSVSQGGYLKIFMEEVPGGSLSSLLRSVWGPLQDNESTISFYTRQILQGLSYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGHPPFHELGSPQAAMFQVGMYKVHPPMPGSLSAEAQAFLLRTFEPDPRLRASAQALLADPFLQPGKRSRSPGSPRQALRSSEAPSASLAPSADSTSQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPGADEPASPEESSGLSLLHQESRRRATLATVLEQELPALAESLRLEQEQGPRLERSHVEQLLLCLRAHIHTPNRRQLAQELRGLQGQLRAQGLEPELLQGPLFAFPDEVKQVLRRRQIRPHWMFVLDSLLSRAVRAALAVLAPEVEEEAVLPKSEEASNAEESEPKQQETPAEPSPLLGEPEQGTPSLMVQLGLLRAETDRLRDVLAEKERECQALVQLALQRVSGEARTCALASEPPAALAVDQGLVQWLQELNVDSGTIQTLLNHSFTLHALLTCATRDDLIYTGIRGGMICRIWRAILAQRTRSTPVTPGPQEAE

Target ORF information:

RefSeq Version	XM_010802602.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802602.3

ORF Insert Sequence:

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG TGAAGTCGCC GGACGTTCCC TCCAGCTGGA CCTTCCAGGT GGCCTCCATC 
AGCGGCGTCA GCGTCTCCAA GCGGGACGAG CGCTGCTGCT TCCTCTACGC GCCTCCTCCG 
GCTCAGGACG TCCAGCTGTG CTTCCCCAGC GCCGGGCACT GCCAGTGGTT CTGCGGCCTG 
ATCCAGGCCT TGGTGAAGAG TCCAGACTCC GGGGCGCCCG CAGAGGAGAC GGAGGGCGCT 
GGGGAGGTGT TGGAGTTTGA TTACGAGTAC ACGGAGTCGG GTGAGCGGCT GGTGCTGGGC 
AAGGGCACGT ACGGGGTGGT GTACGCTGGC CGCGATCGAC ACACTCGCGT GCGCATCGCC 
ATCAAGGAGA TCCCGGAGCG GGACAGCAGG TTCTCTCAGC CGCTGCATGA AGAGATCGCT 
CTGCACAAAC GCCTACGCCA CAAGAACATT GTGCGCTATC TGGGCTCCGT CAGCCAGGGC 
GGCTACCTCA AGATTTTCAT GGAGGAAGTG CCTGGAGGCA GCCTGTCTTC CTTGCTGCGA 
TCGGTGTGGG GACCCCTGCA GGACAACGAG AGCACCATCA GTTTCTACAC CCGCCAGATC 
CTGCAGGGAC TCAGCTACCT GCACGACAAC CACATTGTGC ATCGAGATAT CAAGGGGGAC 
AACGTGCTGA TCAACACCTT CAGCGGGTTG CTCAAGATTT CTGACTTTGG CACCTCCAAG 
CGACTAGCAG GCATCACACC CTGCACTGAG ACCTTCACAG GGACCCTACA ATATATGGCC 
CCAGAAATCA TTGACCAGGG CCCACGAGGG TATGGGAAGG CAGCAGACAT CTGGTCACTG 
GGCTGCACAG TCATCGAGAT GGCCACAGGT CACCCACCCT TCCACGAGCT AGGGAGCCCG 
CAGGCTGCCA TGTTTCAGGT GGGCATGTAC AAGGTGCATC CGCCAATGCC CGGTTCTCTG 
TCAGCTGAGG CTCAAGCCTT CCTCCTCCGA ACTTTTGAGC CCGACCCCCG TCTCCGAGCC 
AGTGCTCAAG CACTGCTGGC AGACCCCTTC CTGCAGCCCG GGAAGAGGAG CCGCAGCCCG 
GGCTCCCCCA GACAGGCTCT AAGGTCCTCA GAGGCGCCAT CCGCAAGCCT TGCTCCTTCA 
GCAGACTCCA CCTCCCAGTC CCAGACATTC CCGCGCCCTC AGGCACCCTC TCAGCACCCT 
CCCAGTCCCC CCAAGCGCTG CCTCAGTTAT GGGGACACCA GCCAGCTCCG GGTGCCCGAG 
GAGCCTGGGG CTGACGAGCC CGCGTCCCCT GAGGAGAGTT CGGGGCTGAG CCTCTTGCAT 
CAAGAGAGTA GGCGCCGGGC CACGCTGGCC ACGGTTCTGG AGCAGGAGCT GCCGGCGCTG 
GCGGAGAGCC TGCGCCTGGA GCAGGAACAG GGTCCCCGTC TGGAGAGGAG TCACGTGGAA 
CAGCTACTGC TCTGCCTCAG GGCGCACATC CATACTCCCA ACCGTCGGCA GCTGGCCCAG 
GAGCTGCGGG GACTCCAAGG GCAGCTTCGG GCCCAGGGCC TTGAGCCCGA GCTTCTGCAA 
GGACCGCTCT TCGCCTTCCC GGATGAGGTG AAGCAGGTCC TCCGCAGGCG CCAGATCCGC 
CCACACTGGA TGTTCGTGCT GGACTCGCTG CTCAGCCGTG CTGTGCGGGC AGCCCTGGCA 
GTGCTGGCCC CAGAGGTGGA GGAGGAGGCG GTCCTACCGA AGTCGGAAGA GGCCAGTAAT 
GCAGAGGAGT CCGAGCCCAA GCAGCAGGAG ACCCCAGCCG AGCCGAGCCC TCTCCTGGGG 
GAACCGGAGC AGGGTACCCC TTCTCTGATG GTGCAGCTGG GCCTCTTGCG AGCAGAGACC 
GATAGGCTTC GAGATGTTCT GGCTGAGAAG GAAAGGGAAT GCCAGGCCTT GGTGCAACTA 
GCCCTCCAGC GGGTCAGTGG GGAGGCCAGG ACCTGTGCCC TGGCTTCAGA GCCCCCAGCG 
GCTCTCGCGG TGGACCAGGG CCTGGTGCAG TGGTTACAGG AACTGAATGT GGATTCAGGC 
ACCATCCAGA CGCTACTGAA CCACAGCTTC ACCCTCCATG CCCTACTGAC CTGTGCCACT 
AGAGATGACC TCATCTACAC AGGCATCAGG GGGGGGATGA TATGCCGCAT CTGGAGAGCC 
ATCTTGGCAC AGCGAACAAG ATCCACGCCA GTTACCCCTG GCCCCCAGGA GGCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K6 ( NM_001193236.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180165.2 Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), mRNA.

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CloneID	OBa216284
Clone ID Related Accession (Same CDS sequence)	NM_001193236.2
Accession Version	NM_001193236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02006350.1. On Jun 30, 2017 this sequence version replaced NM_001193236.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02822103, SAMN04019642 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG ACGTTCCCTC CAGCTGGACC TTCCAGGTGG CCTCCATCAG CGGCGTCAGC 
GTCTCCAAGC GGGACGAGCG CTGCTGCTTC CTCTACGCGC CTCCTCCGGC TCAGGACGTC 
CAGCTGTGCT TCCCCAGCGC CGGGCACTGC CAGTGGTTCT GCGGCCTGAT CCAGGCCTTG 
GTGAAGAGTC CAGACTCCGG GGCGCCCGCA GAGGAGACGG AGGGCGCTGG GGAGGTGTTG 
GAGTTTGATT ACGAGTACAC GGAGTCGGGT GAGCGGCTGG TGCTGGGCAA GGGCACGTAC 
GGGGTGGTGT ACGCTGGCCG CGATCGACAC ACTCGCGTGC GCATCGCCAT CAAGGAGATC 
CCGGAGCGGG ACAGCAGGTT CTCTCAGCCG CTGCATGAAG AGATCGCTCT GCACAAACGC 
CTACGCCACA AGAACATTGT GCGCTATCTG GGCTCCGTCA GCCAGGGCGG CTACCTCAAG 
ATTTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCTTCCT TGCTGCGATC GGTGTGGGGA 
CCCCTGCAGG ACAACGAGAG CACCATCAGT TTCTACACCC GCCAGATCCT GCAGGGACTC 
AGCTACCTGC ACGACAACCA CATTGTGCAT CGAGATATCA AGGGGGACAA CGTGCTGATC 
AACACCTTCA GCGGGTTGCT CAAGATTTCT GACTTTGGCA CCTCCAAGCG ACTAGCAGGC 
ATCACACCCT GCACTGAGAC CTTCACAGGG ACCCTACAAT ATATGGCCCC AGAAATCATT 
GACCAGGGCC CACGAGGGTA TGGGAAGGCA GCAGACATCT GGTCACTGGG CTGCACAGTC 
ATCGAGATGG CCACAGGTCA CCCACCCTTC CACGAGCTAG GGAGCCCGCA GGCTGCCATG 
TTTCAGGTGG GCATGTACAA GGTGCATCCG CCAATGCCCG GTTCTCTGTC AGCTGAGGCT 
CAAGCCTTCC TCCTCCGAAC TTTTGAGCCC GACCCCCGTC TCCGAGCCAG TGCTCAAGCA 
CTGCTGGCAG ACCCCTTCCT GCAGCCCGGG AAGAGGAGCC GCAGCCCGGG CTCCCCCAGA 
CAGGCTCTAA GGTCCTCAGA GGCGCCATCC GCAAGCCTTG CTCCTTCAGC AGACTCCACC 
TCCCAGTCCC AGACATTCCC GCGCCCTCAG GCACCCTCTC AGCACCCTCC CAGTCCCCCC 
AAGCGCTGCC TCAGTTATGG GGACACCAGC CAGCTCCGGG TGCCCGAGGA GCCTGGGGCT 
GACGAGCCCG CGTCCCCTGA GGAGAGTTCG GGGCTGAGCC TCTTGCATCA AGAGAGTAGG 
CGCCGGGCCA CGCTGGCCAC GGTTCTGGAG CAGGAGCTGC CGGCGCTGGC GGAGAGCCTG 
CGCCTGGAGC AGGAACAGGA CCCTTCTTTT CCTTCCCAGG GTCCCCGTCT GGAGAGGAGT 
CACGTGGAAC AGCTACTGCT CTGCCTCAGG GCGCACATCC ATACTCCCAA CCGTCGGCAG 
CTGGCCCAGG AGCTGCGGGG ACTCCAAGGG CAGCTTCGGG CCCAGGGCCT TGAGCCCGAG 
CTTCTGCAAG GACCGCTCTT CGCCTTCCCG GATGAGGTGA AGCAGGTCCT CCGCAGGCGC 
CAGATCCGCC CACACTGGAT GTTCGTGCTG GACTCGCTGC TCAGCCGTGC TGTGCGGGCA 
GCCCTGGCAG TGCTGGCCCC AGAGGTGGAG GAGGAGGCGG TCCTACCGAA GTCGGAAGAG 
GCCAGTAATG CAGAGGAGTC CGAGCCCAAG CAGCAGGAGA CCCCAGCCGA GCCGAGCCCT 
CTCCTGGGGG AACCGGAGCA GGGTACCCCT TCTCTGATGG TGCAGCTGGG CCTCTTGCGA 
GCAGAGACCG ATAGGCTTCG AGATGTTCTG GCTGAGAAGG AAAGGGAATG CCAGGCCTTG 
GTGCAACTAG CCCTCCAGCG GGTCAGTGGG GAGGCCAGGA CCTGTGCCCT GGCTTCAGAG 
CCCCCAGCGG CTCTCGCGGT GGACCAGGGC CTGGTGCAGT GGTTACAGGA ACTGAATGTG 
GATTCAGGCA CCATCCAGAC GCTACTGAAC CACAGAAAAA ACCTCCATGC CCTACTGACC 
TGTGCCACTA GAGATGACCT CATCTACACA GGCATCAGGG GGGGGATGAT ATGCCGCATC 
TGGAGAGCCA TCTTGGCACA GCGAACAAGA TCCACGCCAG TTACCCCTGG CCCCCAGGAG 
GCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180165.2
CDS	1..3909
Translation	MAGPSPRSGALERAGSCWQDPLAEALSRGRPLAASPGRGCARSRPLSVVYVLTREPLPEVEPETGAEAEPLPLRCLREACAQLPGPRPRPQLHSLPFGTLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDIFQKNSDCIGSYTLIPYVVTATGRVLCGDAGLLRGLADGLLQAGVGTEALLTPLVGRLAHLLEATPTDSCGYFRETIRQDIRRARERFSGLQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIDLVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVQVEGPVAPDLYCMCGRIYKDMFFSSRFQDAGHREQAYHWYRKAFDIEPSLHSGINAAVLLIADGQRFEDSEELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDLTQVALAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTLELPGEAPHGAHFWLHFLLQSCQPLKTACPQGDQCLVLVLEMNKVLLPARLEVQGTNPVSAVTLSLLEPEKQDVPSSWTFQVASISGVSVSKRDERCCFLYAPPPAQDVQLCFPSAGHCQWFCGLIQALVKSPDSGAPAEETEGAGEVLEFDYEYTESGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSVSQGGYLKIFMEEVPGGSLSSLLRSVWGPLQDNESTISFYTRQILQGLSYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGHPPFHELGSPQAAMFQVGMYKVHPPMPGSLSAEAQAFLLRTFEPDPRLRASAQALLADPFLQPGKRSRSPGSPRQALRSSEAPSASLAPSADSTSQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPGADEPASPEESSGLSLLHQESRRRATLATVLEQELPALAESLRLEQEQDPSFPSQGPRLERSHVEQLLLCLRAHIHTPNRRQLAQELRGLQGQLRAQGLEPELLQGPLFAFPDEVKQVLRRRQIRPHWMFVLDSLLSRAVRAALAVLAPEVEEEAVLPKSEEASNAEESEPKQQETPAEPSPLLGEPEQGTPSLMVQLGLLRAETDRLRDVLAEKERECQALVQLALQRVSGEARTCALASEPPAALAVDQGLVQWLQELNVDSGTIQTLLNHRKNLHALLTCATRDDLIYTGIRGGMICRIWRAILAQRTRSTPVTPGPQEAE

Target ORF information:

RefSeq Version	NM_001193236.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193236.2

ORF Insert Sequence:

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG ACGTTCCCTC CAGCTGGACC TTCCAGGTGG CCTCCATCAG CGGCGTCAGC 
GTCTCCAAGC GGGACGAGCG CTGCTGCTTC CTCTACGCGC CTCCTCCGGC TCAGGACGTC 
CAGCTGTGCT TCCCCAGCGC CGGGCACTGC CAGTGGTTCT GCGGCCTGAT CCAGGCCTTG 
GTGAAGAGTC CAGACTCCGG GGCGCCCGCA GAGGAGACGG AGGGCGCTGG GGAGGTGTTG 
GAGTTTGATT ACGAGTACAC GGAGTCGGGT GAGCGGCTGG TGCTGGGCAA GGGCACGTAC 
GGGGTGGTGT ACGCTGGCCG CGATCGACAC ACTCGCGTGC GCATCGCCAT CAAGGAGATC 
CCGGAGCGGG ACAGCAGGTT CTCTCAGCCG CTGCATGAAG AGATCGCTCT GCACAAACGC 
CTACGCCACA AGAACATTGT GCGCTATCTG GGCTCCGTCA GCCAGGGCGG CTACCTCAAG 
ATTTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCTTCCT TGCTGCGATC GGTGTGGGGA 
CCCCTGCAGG ACAACGAGAG CACCATCAGT TTCTACACCC GCCAGATCCT GCAGGGACTC 
AGCTACCTGC ACGACAACCA CATTGTGCAT CGAGATATCA AGGGGGACAA CGTGCTGATC 
AACACCTTCA GCGGGTTGCT CAAGATTTCT GACTTTGGCA CCTCCAAGCG ACTAGCAGGC 
ATCACACCCT GCACTGAGAC CTTCACAGGG ACCCTACAAT ATATGGCCCC AGAAATCATT 
GACCAGGGCC CACGAGGGTA TGGGAAGGCA GCAGACATCT GGTCACTGGG CTGCACAGTC 
ATCGAGATGG CCACAGGTCA CCCACCCTTC CACGAGCTAG GGAGCCCGCA GGCTGCCATG 
TTTCAGGTGG GCATGTACAA GGTGCATCCG CCAATGCCCG GTTCTCTGTC AGCTGAGGCT 
CAAGCCTTCC TCCTCCGAAC TTTTGAGCCC GACCCCCGTC TCCGAGCCAG TGCTCAAGCA 
CTGCTGGCAG ACCCCTTCCT GCAGCCCGGG AAGAGGAGCC GCAGCCCGGG CTCCCCCAGA 
CAGGCTCTAA GGTCCTCAGA GGCGCCATCC GCAAGCCTTG CTCCTTCAGC AGACTCCACC 
TCCCAGTCCC AGACATTCCC GCGCCCTCAG GCACCCTCTC AGCACCCTCC CAGTCCCCCC 
AAGCGCTGCC TCAGTTATGG GGACACCAGC CAGCTCCGGG TGCCCGAGGA GCCTGGGGCT 
GACGAGCCCG CGTCCCCTGA GGAGAGTTCG GGGCTGAGCC TCTTGCATCA AGAGAGTAGG 
CGCCGGGCCA CGCTGGCCAC GGTTCTGGAG CAGGAGCTGC CGGCGCTGGC GGAGAGCCTG 
CGCCTGGAGC AGGAACAGGA CCCTTCTTTT CCTTCCCAGG GTCCCCGTCT GGAGAGGAGT 
CACGTGGAAC AGCTACTGCT CTGCCTCAGG GCGCACATCC ATACTCCCAA CCGTCGGCAG 
CTGGCCCAGG AGCTGCGGGG ACTCCAAGGG CAGCTTCGGG CCCAGGGCCT TGAGCCCGAG 
CTTCTGCAAG GACCGCTCTT CGCCTTCCCG GATGAGGTGA AGCAGGTCCT CCGCAGGCGC 
CAGATCCGCC CACACTGGAT GTTCGTGCTG GACTCGCTGC TCAGCCGTGC TGTGCGGGCA 
GCCCTGGCAG TGCTGGCCCC AGAGGTGGAG GAGGAGGCGG TCCTACCGAA GTCGGAAGAG 
GCCAGTAATG CAGAGGAGTC CGAGCCCAAG CAGCAGGAGA CCCCAGCCGA GCCGAGCCCT 
CTCCTGGGGG AACCGGAGCA GGGTACCCCT TCTCTGATGG TGCAGCTGGG CCTCTTGCGA 
GCAGAGACCG ATAGGCTTCG AGATGTTCTG GCTGAGAAGG AAAGGGAATG CCAGGCCTTG 
GTGCAACTAG CCCTCCAGCG GGTCAGTGGG GAGGCCAGGA CCTGTGCCCT GGCTTCAGAG 
CCCCCAGCGG CTCTCGCGGT GGACCAGGGC CTGGTGCAGT GGTTACAGGA ACTGAATGTG 
GATTCAGGCA CCATCCAGAC GCTACTGAAC CACAGAAAAA ACCTCCATGC CCTACTGACC 
TGTGCCACTA GAGATGACCT CATCTACACA GGCATCAGGG GGGGGATGAT ATGCCGCATC 
TGGAGAGCCA TCTTGGCACA GCGAACAAGA TCCACGCCAG TTACCCCTGG CCCCCAGGAG 
GCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K6 ( NM_001193236.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180165.3 Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), mRNA.

Price & Availability↑

CloneID	OBa40511
Clone ID Related Accession (Same CDS sequence)	NM_001193236.3 , NM_001193236.1
Accession Version	NM_001193236.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On May 29, 2018 this sequence version replaced NM_001193236.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145433, SAMN03145439 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG ACGTTCCCTC CAGCTGGACC TTCCAGGTGG CCTCCATCAG CGGCGTCAGC 
GTCTCCAAGC GGGACGAGCG CTGCTGCTTC CTCTACGCGC CTCCTCCGGC TCAGGACGTC 
CAGCTGTGCT TCCCCAGCGC CGGGCACTGC CAGTGGTTCT GCGGCCTGAT CCAGGCCTTG 
GTGAAGAGTC CAGACTCCGG GGCGCCCGCA GAGGAGACGG AGGGCGCTGG GGAGGTGTTG 
GAGTTTGATT ACGAGTACAC GGAGTCGGGT GAGCGGCTGG TGCTGGGCAA GGGCACGTAC 
GGGGTGGTGT ACGCTGGCCG CGATCGACAC ACTCGCGTGC GCATCGCCAT CAAGGAGATC 
CCGGAGCGGG ACAGCAGGTT CTCTCAGCCG CTGCATGAAG AGATCGCTCT GCACAAACGC 
CTACGCCACA AGAACATTGT GCGCTATCTG GGCTCCGTCA GCCAGGGCGG CTACCTCAAG 
ATTTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCTTCCT TGCTGCGATC GGTGTGGGGA 
CCCCTGCAGG ACAACGAGAG CACCATCAGT TTCTACACCC GCCAGATCCT GCAGGGACTC 
AGCTACCTGC ACGACAACCA CATTGTGCAT CGAGATATCA AGGGGGACAA CGTGCTGATC 
AACACCTTCA GCGGGTTGCT CAAGATTTCT GACTTTGGCA CCTCCAAGCG ACTAGCAGGC 
ATCACACCCT GCACTGAGAC CTTCACAGGG ACCCTACAAT ATATGGCCCC AGAAATCATT 
GACCAGGGCC CACGAGGGTA TGGGAAGGCA GCAGACATCT GGTCACTGGG CTGCACAGTC 
ATCGAGATGG CCACAGGTCA CCCACCCTTC CACGAGCTAG GGAGCCCGCA GGCTGCCATG 
TTTCAGGTGG GCATGTACAA GGTGCATCCG CCAATGCCCG GTTCTCTGTC AGCTGAGGCT 
CAAGCCTTCC TCCTCCGAAC TTTTGAGCCC GACCCCCGTC TCCGAGCCAG TGCTCAAGCA 
CTGCTGGCAG ACCCCTTCCT GCAGCCCGGG AAGAGGAGCC GCAGCCCGGG CTCCCCCAGA 
CAGGCTCTAA GGTCCTCAGA GGCGCCATCC GCAAGCCTTG CTCCTTCAGC AGACTCCACC 
TCCCAGTCCC AGACATTCCC GCGCCCTCAG GCACCCTCTC AGCACCCTCC CAGTCCCCCC 
AAGCGCTGCC TCAGTTATGG GGACACCAGC CAGCTCCGGG TGCCCGAGGA GCCTGGGGCT 
GACGAGCCCG CGTCCCCTGA GGAGAGTTCG GGGCTGAGCC TCTTGCATCA AGAGAGTAGG 
CGCCGGGCCA CGCTGGCCAC GGTTCTGGAG CAGGAGCTGC CGGCGCTGGC GGAGAGCCTG 
CGCCTGGAGC AGGAACAGGA CCCTTCTTTT CCTTCCCAGG GTCCCCGTCT GGAGAGGAGT 
CACGTGGAAC AGCTACTGCT CTGCCTCAGG GCGCACATCC ATACTCCCAA CCGTCGGCAG 
CTGGCCCAGG AGCTGCGGGG ACTCCAAGGG CAGCTTCGGG CCCAGGGCCT TGAGCCCGAG 
CTTCTGCAAG GACCGCTCTT CGCCTTCCCG GATGAGGTGA AGCAGGTCCT CCGCAGGCGC 
CAGATCCGCC CACACTGGAT GTTCGTGCTG GACTCGCTGC TCAGCCGTGC TGTGCGGGCA 
GCCCTGGCAG TGCTGGCCCC AGAGGTGGAG GAGGAGGCGG TCCTACCGAA GTCGGAAGAG 
GCCAGTAATG CAGAGGAGTC CGAGCCCAAG CAGCAGGAGA CCCCAGCCGA GCCGAGCCCT 
CTCCTGGGGG AACCGGAGCA GGGTACCCCT TCTCTGATGG TGCAGCTGGG CCTCTTGCGA 
GCAGAGACCG ATAGGCTTCG AGATGTTCTG GCTGAGAAGG AAAGGGAATG CCAGGCCTTG 
GTGCAACTAG CCCTCCAGCG GGTCAGTGGG GAGGCCAGGA CCTGTGCCCT GGCTTCAGAG 
CCCCCAGCGG CTCTCGCGGT GGACCAGGGC CTGGTGCAGT GGTTACAGGA ACTGAATGTG 
GATTCAGGCA CCATCCAGAC GCTACTGAAC CACAGCTTCA CCCTCCATGC CCTACTGACC 
TGTGCCACTA GAGATGACCT CATCTACACA GGCATCAGGG GGGGGATGAT ATGCCGCATC 
TGGAGAGCCA TCTTGGCACA GCGAACAAGA TCCACGCCAG TTACCCCTGG CCCCCAGGAG 
GCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180165.3
CDS	1..3909
Translation	MAGPSPRSGALERAGSCWQDPLAEALSRGRPLAASPGRGCARSRPLSVVYVLTREPLPEVEPETGAEAEPLPLRCLREACAQLPGPRPRPQLHSLPFGTLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDIFQKNSDCIGSYTLIPYVVTATGRVLCGDAGLLRGLADGLLQAGVGTEALLTPLVGRLAHLLEATPTDSCGYFRETIRQDIRRARERFSGLQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIDLVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVQVEGPVAPDLYCMCGRIYKDMFFSSRFQDAGHREQAYHWYRKAFDIEPSLHSGINAAVLLIADGQRFEDSEELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDLTQVALAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTLELPGEAPHGAHFWLHFLLQSCQPLKTACPQGDQCLVLVLEMNKVLLPARLEVQGTNPVSAVTLSLLEPEKQDVPSSWTFQVASISGVSVSKRDERCCFLYAPPPAQDVQLCFPSAGHCQWFCGLIQALVKSPDSGAPAEETEGAGEVLEFDYEYTESGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSVSQGGYLKIFMEEVPGGSLSSLLRSVWGPLQDNESTISFYTRQILQGLSYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGHPPFHELGSPQAAMFQVGMYKVHPPMPGSLSAEAQAFLLRTFEPDPRLRASAQALLADPFLQPGKRSRSPGSPRQALRSSEAPSASLAPSADSTSQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPGADEPASPEESSGLSLLHQESRRRATLATVLEQELPALAESLRLEQEQDPSFPSQGPRLERSHVEQLLLCLRAHIHTPNRRQLAQELRGLQGQLRAQGLEPELLQGPLFAFPDEVKQVLRRRQIRPHWMFVLDSLLSRAVRAALAVLAPEVEEEAVLPKSEEASNAEESEPKQQETPAEPSPLLGEPEQGTPSLMVQLGLLRAETDRLRDVLAEKERECQALVQLALQRVSGEARTCALASEPPAALAVDQGLVQWLQELNVDSGTIQTLLNHSFTLHALLTCATRDDLIYTGIRGGMICRIWRAILAQRTRSTPVTPGPQEAE

Target ORF information:

RefSeq Version	NM_001193236.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193236.3

ORF Insert Sequence:

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG ACGTTCCCTC CAGCTGGACC TTCCAGGTGG CCTCCATCAG CGGCGTCAGC 
GTCTCCAAGC GGGACGAGCG CTGCTGCTTC CTCTACGCGC CTCCTCCGGC TCAGGACGTC 
CAGCTGTGCT TCCCCAGCGC CGGGCACTGC CAGTGGTTCT GCGGCCTGAT CCAGGCCTTG 
GTGAAGAGTC CAGACTCCGG GGCGCCCGCA GAGGAGACGG AGGGCGCTGG GGAGGTGTTG 
GAGTTTGATT ACGAGTACAC GGAGTCGGGT GAGCGGCTGG TGCTGGGCAA GGGCACGTAC 
GGGGTGGTGT ACGCTGGCCG CGATCGACAC ACTCGCGTGC GCATCGCCAT CAAGGAGATC 
CCGGAGCGGG ACAGCAGGTT CTCTCAGCCG CTGCATGAAG AGATCGCTCT GCACAAACGC 
CTACGCCACA AGAACATTGT GCGCTATCTG GGCTCCGTCA GCCAGGGCGG CTACCTCAAG 
ATTTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCTTCCT TGCTGCGATC GGTGTGGGGA 
CCCCTGCAGG ACAACGAGAG CACCATCAGT TTCTACACCC GCCAGATCCT GCAGGGACTC 
AGCTACCTGC ACGACAACCA CATTGTGCAT CGAGATATCA AGGGGGACAA CGTGCTGATC 
AACACCTTCA GCGGGTTGCT CAAGATTTCT GACTTTGGCA CCTCCAAGCG ACTAGCAGGC 
ATCACACCCT GCACTGAGAC CTTCACAGGG ACCCTACAAT ATATGGCCCC AGAAATCATT 
GACCAGGGCC CACGAGGGTA TGGGAAGGCA GCAGACATCT GGTCACTGGG CTGCACAGTC 
ATCGAGATGG CCACAGGTCA CCCACCCTTC CACGAGCTAG GGAGCCCGCA GGCTGCCATG 
TTTCAGGTGG GCATGTACAA GGTGCATCCG CCAATGCCCG GTTCTCTGTC AGCTGAGGCT 
CAAGCCTTCC TCCTCCGAAC TTTTGAGCCC GACCCCCGTC TCCGAGCCAG TGCTCAAGCA 
CTGCTGGCAG ACCCCTTCCT GCAGCCCGGG AAGAGGAGCC GCAGCCCGGG CTCCCCCAGA 
CAGGCTCTAA GGTCCTCAGA GGCGCCATCC GCAAGCCTTG CTCCTTCAGC AGACTCCACC 
TCCCAGTCCC AGACATTCCC GCGCCCTCAG GCACCCTCTC AGCACCCTCC CAGTCCCCCC 
AAGCGCTGCC TCAGTTATGG GGACACCAGC CAGCTCCGGG TGCCCGAGGA GCCTGGGGCT 
GACGAGCCCG CGTCCCCTGA GGAGAGTTCG GGGCTGAGCC TCTTGCATCA AGAGAGTAGG 
CGCCGGGCCA CGCTGGCCAC GGTTCTGGAG CAGGAGCTGC CGGCGCTGGC GGAGAGCCTG 
CGCCTGGAGC AGGAACAGGA CCCTTCTTTT CCTTCCCAGG GTCCCCGTCT GGAGAGGAGT 
CACGTGGAAC AGCTACTGCT CTGCCTCAGG GCGCACATCC ATACTCCCAA CCGTCGGCAG 
CTGGCCCAGG AGCTGCGGGG ACTCCAAGGG CAGCTTCGGG CCCAGGGCCT TGAGCCCGAG 
CTTCTGCAAG GACCGCTCTT CGCCTTCCCG GATGAGGTGA AGCAGGTCCT CCGCAGGCGC 
CAGATCCGCC CACACTGGAT GTTCGTGCTG GACTCGCTGC TCAGCCGTGC TGTGCGGGCA 
GCCCTGGCAG TGCTGGCCCC AGAGGTGGAG GAGGAGGCGG TCCTACCGAA GTCGGAAGAG 
GCCAGTAATG CAGAGGAGTC CGAGCCCAAG CAGCAGGAGA CCCCAGCCGA GCCGAGCCCT 
CTCCTGGGGG AACCGGAGCA GGGTACCCCT TCTCTGATGG TGCAGCTGGG CCTCTTGCGA 
GCAGAGACCG ATAGGCTTCG AGATGTTCTG GCTGAGAAGG AAAGGGAATG CCAGGCCTTG 
GTGCAACTAG CCCTCCAGCG GGTCAGTGGG GAGGCCAGGA CCTGTGCCCT GGCTTCAGAG 
CCCCCAGCGG CTCTCGCGGT GGACCAGGGC CTGGTGCAGT GGTTACAGGA ACTGAATGTG 
GATTCAGGCA CCATCCAGAC GCTACTGAAC CACAGCTTCA CCCTCCATGC CCTACTGACC 
TGTGCCACTA GAGATGACCT CATCTACACA GGCATCAGGG GGGGGATGAT ATGCCGCATC 
TGGAGAGCCA TCTTGGCACA GCGAACAAGA TCCACGCCAG TTACCCCTGG CCCCCAGGAG 
GCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K6 ( NM_001193236.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180165.1 Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), mRNA.

Price & Availability↑

CloneID	OBa40511
Clone ID Related Accession (Same CDS sequence)	NM_001193236.3 , NM_001193236.1
Accession Version	NM_001193236.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	mitogen-activated protein kinase kinase kinase 6
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03025488.1 and AAFC03025484.1. On Jul 17, 2010 this sequence version replaced XM_877401.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01093751, SAMN02404551 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG ACGTTCCCTC CAGCTGGACC TTCCAGGTGG CCTCCATCAG CGGCGTCAGC 
GTCTCCAAGC GGGACGAGCG CTGCTGCTTC CTCTACGCGC CTCCTCCGGC TCAGGACGTC 
CAGCTGTGCT TCCCCAGCGC CGGGCACTGC CAGTGGTTCT GCGGCCTGAT CCAGGCCTTG 
GTGAAGAGTC CAGACTCCGG GGCGCCCGCA GAGGAGACGG AGGGCGCTGG GGAGGTGTTG 
GAGTTTGATT ACGAGTACAC GGAGTCGGGT GAGCGGCTGG TGCTGGGCAA GGGCACGTAC 
GGGGTGGTGT ACGCTGGCCG CGATCGACAC ACTCGCGTGC GCATCGCCAT CAAGGAGATC 
CCGGAGCGGG ACAGCAGGTT CTCTCAGCCG CTGCATGAAG AGATCGCTCT GCACAAACGC 
CTACGCCACA AGAACATTGT GCGCTATCTG GGCTCCGTCA GCCAGGGCGG CTACCTCAAG 
ATTTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCTTCCT TGCTGCGATC GGTGTGGGGA 
CCCCTGCAGG ACAACGAGAG CACCATCAGT TTCTACACCC GCCAGATCCT GCAGGGACTC 
AGCTACCTGC ACGACAACCA CATTGTGCAT CGAGATATCA AGGGGGACAA CGTGCTGATC 
AACACCTTCA GCGGGTTGCT CAAGATTTCT GACTTTGGCA CCTCCAAGCG ACTAGCAGGC 
ATCACACCCT GCACTGAGAC CTTCACAGGG ACCCTACAAT ATATGGCCCC AGAAATCATT 
GACCAGGGCC CACGAGGGTA TGGGAAGGCA GCAGACATCT GGTCACTGGG CTGCACAGTC 
ATCGAGATGG CCACAGGTCA CCCACCCTTC CACGAGCTAG GGAGCCCGCA GGCTGCCATG 
TTTCAGGTGG GCATGTACAA GGTGCATCCG CCAATGCCCG GTTCTCTGTC AGCTGAGGCT 
CAAGCCTTCC TCCTCCGAAC TTTTGAGCCC GACCCCCGTC TCCGAGCCAG TGCTCAAGCA 
CTGCTGGCAG ACCCCTTCCT GCAGCCCGGG AAGAGGAGCC GCAGCCCGGG CTCCCCCAGA 
CAGGCTCTAA GGTCCTCAGA GGCGCCATCC GCAAGCCTTG CTCCTTCAGC AGACTCCACC 
TCCCAGTCCC AGACATTCCC GCGCCCTCAG GCACCCTCTC AGCACCCTCC CAGTCCCCCC 
AAGCGCTGCC TCAGTTATGG GGACACCAGC CAGCTCCGGG TGCCCGAGGA GCCTGGGGCT 
GACGAGCCCG CGTCCCCTGA GGAGAGTTCG GGGCTGAGCC TCTTGCATCA AGAGAGTAGG 
CGCCGGGCCA CGCTGGCCAC GGTTCTGGAG CAGGAGCTGC CGGCGCTGGC GGAGAGCCTG 
CGCCTGGAGC AGGAACAGGA CCCTTCTTTT CCTTCCCAGG GTCCCCGTCT GGAGAGGAGT 
CACGTGGAAC AGCTACTGCT CTGCCTCAGG GCGCACATCC ATACTCCCAA CCGTCGGCAG 
CTGGCCCAGG AGCTGCGGGG ACTCCAAGGG CAGCTTCGGG CCCAGGGCCT TGAGCCCGAG 
CTTCTGCAAG GACCGCTCTT CGCCTTCCCG GATGAGGTGA AGCAGGTCCT CCGCAGGCGC 
CAGATCCGCC CACACTGGAT GTTCGTGCTG GACTCGCTGC TCAGCCGTGC TGTGCGGGCA 
GCCCTGGCAG TGCTGGCCCC AGAGGTGGAG GAGGAGGCGG TCCTACCGAA GTCGGAAGAG 
GCCAGTAATG CAGAGGAGTC CGAGCCCAAG CAGCAGGAGA CCCCAGCCGA GCCGAGCCCT 
CTCCTGGGGG AACCGGAGCA GGGTACCCCT TCTCTGATGG TGCAGCTGGG CCTCTTGCGA 
GCAGAGACCG ATAGGCTTCG AGATGTTCTG GCTGAGAAGG AAAGGGAATG CCAGGCCTTG 
GTGCAACTAG CCCTCCAGCG GGTCAGTGGG GAGGCCAGGA CCTGTGCCCT GGCTTCAGAG 
CCCCCAGCGG CTCTCGCGGT GGACCAGGGC CTGGTGCAGT GGTTACAGGA ACTGAATGTG 
GATTCAGGCA CCATCCAGAC GCTACTGAAC CACAGCTTCA CCCTCCATGC CCTACTGACC 
TGTGCCACTA GAGATGACCT CATCTACACA GGCATCAGGG GGGGGATGAT ATGCCGCATC 
TGGAGAGCCA TCTTGGCACA GCGAACAAGA TCCACGCCAG TTACCCCTGG CCCCCAGGAG 
GCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180165.1
CDS	1..3909
Translation	MAGPSPRSGALERAGSCWQDPLAEALSRGRPLAASPGRGCARSRPLSVVYVLTREPLPEVEPETGAEAEPLPLRCLREACAQLPGPRPRPQLHSLPFGTLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDIFQKNSDCIGSYTLIPYVVTATGRVLCGDAGLLRGLADGLLQAGVGTEALLTPLVGRLAHLLEATPTDSCGYFRETIRQDIRRARERFSGLQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIDLVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVQVEGPVAPDLYCMCGRIYKDMFFSSRFQDAGHREQAYHWYRKAFDIEPSLHSGINAAVLLIADGQRFEDSEELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDLTQVALAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTLELPGEAPHGAHFWLHFLLQSCQPLKTACPQGDQCLVLVLEMNKVLLPARLEVQGTNPVSAVTLSLLEPEKQDVPSSWTFQVASISGVSVSKRDERCCFLYAPPPAQDVQLCFPSAGHCQWFCGLIQALVKSPDSGAPAEETEGAGEVLEFDYEYTESGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSVSQGGYLKIFMEEVPGGSLSSLLRSVWGPLQDNESTISFYTRQILQGLSYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGHPPFHELGSPQAAMFQVGMYKVHPPMPGSLSAEAQAFLLRTFEPDPRLRASAQALLADPFLQPGKRSRSPGSPRQALRSSEAPSASLAPSADSTSQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPGADEPASPEESSGLSLLHQESRRRATLATVLEQELPALAESLRLEQEQDPSFPSQGPRLERSHVEQLLLCLRAHIHTPNRRQLAQELRGLQGQLRAQGLEPELLQGPLFAFPDEVKQVLRRRQIRPHWMFVLDSLLSRAVRAALAVLAPEVEEEAVLPKSEEASNAEESEPKQQETPAEPSPLLGEPEQGTPSLMVQLGLLRAETDRLRDVLAEKERECQALVQLALQRVSGEARTCALASEPPAALAVDQGLVQWLQELNVDSGTIQTLLNHSFTLHALLTCATRDDLIYTGIRGGMICRIWRAILAQRTRSTPVTPGPQEAE

Target ORF information:

RefSeq Version	NM_001193236.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193236.1

ORF Insert Sequence:

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG ACGTTCCCTC CAGCTGGACC TTCCAGGTGG CCTCCATCAG CGGCGTCAGC 
GTCTCCAAGC GGGACGAGCG CTGCTGCTTC CTCTACGCGC CTCCTCCGGC TCAGGACGTC 
CAGCTGTGCT TCCCCAGCGC CGGGCACTGC CAGTGGTTCT GCGGCCTGAT CCAGGCCTTG 
GTGAAGAGTC CAGACTCCGG GGCGCCCGCA GAGGAGACGG AGGGCGCTGG GGAGGTGTTG 
GAGTTTGATT ACGAGTACAC GGAGTCGGGT GAGCGGCTGG TGCTGGGCAA GGGCACGTAC 
GGGGTGGTGT ACGCTGGCCG CGATCGACAC ACTCGCGTGC GCATCGCCAT CAAGGAGATC 
CCGGAGCGGG ACAGCAGGTT CTCTCAGCCG CTGCATGAAG AGATCGCTCT GCACAAACGC 
CTACGCCACA AGAACATTGT GCGCTATCTG GGCTCCGTCA GCCAGGGCGG CTACCTCAAG 
ATTTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCTTCCT TGCTGCGATC GGTGTGGGGA 
CCCCTGCAGG ACAACGAGAG CACCATCAGT TTCTACACCC GCCAGATCCT GCAGGGACTC 
AGCTACCTGC ACGACAACCA CATTGTGCAT CGAGATATCA AGGGGGACAA CGTGCTGATC 
AACACCTTCA GCGGGTTGCT CAAGATTTCT GACTTTGGCA CCTCCAAGCG ACTAGCAGGC 
ATCACACCCT GCACTGAGAC CTTCACAGGG ACCCTACAAT ATATGGCCCC AGAAATCATT 
GACCAGGGCC CACGAGGGTA TGGGAAGGCA GCAGACATCT GGTCACTGGG CTGCACAGTC 
ATCGAGATGG CCACAGGTCA CCCACCCTTC CACGAGCTAG GGAGCCCGCA GGCTGCCATG 
TTTCAGGTGG GCATGTACAA GGTGCATCCG CCAATGCCCG GTTCTCTGTC AGCTGAGGCT 
CAAGCCTTCC TCCTCCGAAC TTTTGAGCCC GACCCCCGTC TCCGAGCCAG TGCTCAAGCA 
CTGCTGGCAG ACCCCTTCCT GCAGCCCGGG AAGAGGAGCC GCAGCCCGGG CTCCCCCAGA 
CAGGCTCTAA GGTCCTCAGA GGCGCCATCC GCAAGCCTTG CTCCTTCAGC AGACTCCACC 
TCCCAGTCCC AGACATTCCC GCGCCCTCAG GCACCCTCTC AGCACCCTCC CAGTCCCCCC 
AAGCGCTGCC TCAGTTATGG GGACACCAGC CAGCTCCGGG TGCCCGAGGA GCCTGGGGCT 
GACGAGCCCG CGTCCCCTGA GGAGAGTTCG GGGCTGAGCC TCTTGCATCA AGAGAGTAGG 
CGCCGGGCCA CGCTGGCCAC GGTTCTGGAG CAGGAGCTGC CGGCGCTGGC GGAGAGCCTG 
CGCCTGGAGC AGGAACAGGA CCCTTCTTTT CCTTCCCAGG GTCCCCGTCT GGAGAGGAGT 
CACGTGGAAC AGCTACTGCT CTGCCTCAGG GCGCACATCC ATACTCCCAA CCGTCGGCAG 
CTGGCCCAGG AGCTGCGGGG ACTCCAAGGG CAGCTTCGGG CCCAGGGCCT TGAGCCCGAG 
CTTCTGCAAG GACCGCTCTT CGCCTTCCCG GATGAGGTGA AGCAGGTCCT CCGCAGGCGC 
CAGATCCGCC CACACTGGAT GTTCGTGCTG GACTCGCTGC TCAGCCGTGC TGTGCGGGCA 
GCCCTGGCAG TGCTGGCCCC AGAGGTGGAG GAGGAGGCGG TCCTACCGAA GTCGGAAGAG 
GCCAGTAATG CAGAGGAGTC CGAGCCCAAG CAGCAGGAGA CCCCAGCCGA GCCGAGCCCT 
CTCCTGGGGG AACCGGAGCA GGGTACCCCT TCTCTGATGG TGCAGCTGGG CCTCTTGCGA 
GCAGAGACCG ATAGGCTTCG AGATGTTCTG GCTGAGAAGG AAAGGGAATG CCAGGCCTTG 
GTGCAACTAG CCCTCCAGCG GGTCAGTGGG GAGGCCAGGA CCTGTGCCCT GGCTTCAGAG 
CCCCCAGCGG CTCTCGCGGT GGACCAGGGC CTGGTGCAGT GGTTACAGGA ACTGAATGTG 
GATTCAGGCA CCATCCAGAC GCTACTGAAC CACAGCTTCA CCCTCCATGC CCTACTGACC 
TGTGCCACTA GAGATGACCT CATCTACACA GGCATCAGGG GGGGGATGAT ATGCCGCATC 
TGGAGAGCCA TCTTGGCACA GCGAACAAGA TCCACGCCAG TTACCCCTGG CCCCCAGGAG 
GCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K6 ( XM_005203237.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203294.1 Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X1, mRNA.

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CloneID	OBa16997
Clone ID Related Accession (Same CDS sequence)	XM_005203237.3
Accession Version	XM_005203237.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG TGAAGTCGCC GGACGTTCCC TCCAGCTGGA CCTTCCAGGT GGCCTCCATC 
AGCGGCGTCA GCGTCTCCAA GCGGGACGAG CGCTGCTGCT TCCTCTACGC GCCTCCTCCG 
GCTCAGGACG TCCAGCTGTG CTTCCCCAGC GCCGGGCACT GCCAGTGGTT CTGCGGCCTG 
ATCCAGGCCT TGGTGAAGAG TCCAGACTCC GGGGCGCCCG CAGAGGAGAC GGAGGGCGCT 
GGGGAGGTGT TGGAGTTTGA TTACGAGTAC ACGGAGTCGG GTGAGCGGCT GGTGCTGGGC 
AAGGGCACGT ACGGGGTGGT GTACGCTGGC CGCGATCGAC ACACTCGCGT GCGCATCGCC 
ATCAAGGAGA TCCCGGAGCG GGACAGCAGG TTCTCTCAGC CGCTGCATGA AGAGATCGCT 
CTGCACAAAC GCCTACGCCA CAAGAACATT GTGCGCTATC TGGGCTCCGT CAGCCAGGGC 
GGCTACCTCA AGATTTTCAT GGAGGAAGTG CCTGGAGGCA GCCTGTCTTC CTTGCTGCGA 
TCGGTGTGGG GACCCCTGCA GGACAACGAG AGCACCATCA GTTTCTACAC CCGCCAGATC 
CTGCAGGGAC TCAGCTACCT GCACGACAAC CACATTGTGC ATCGAGATAT CAAGGGGGAC 
AACGTGCTGA TCAACACCTT CAGCGGGTTG CTCAAGATTT CTGACTTTGG CACCTCCAAG 
CGACTAGCAG GCATCACACC CTGCACTGAG ACCTTCACAG GGACCCTACA ATATATGGCC 
CCAGAAATCA TTGACCAGGG CCCACGAGGG TATGGGAAGG CAGCAGACAT CTGGTCACTG 
GGCTGCACAG TCATCGAGAT GGCCACAGGT CACCCACCCT TCCACGAGCT AGGGAGCCCG 
CAGGCTGCCA TGTTTCAGGT GGGCATGTAC AAGGTGCATC CGCCAATGCC CGGTTCTCTG 
TCAGCTGAGG CTCAAGCCTT CCTCCTCCGA ACTTTTGAGC CCGACCCCCG TCTCCGAGCC 
AGTGCTCAAG CACTGCTGGC AGACCCCTTC CTGCAGCCCG GGAAGAGGAG CCGCAGCCCG 
GGCTCCCCCA GACAGGCTCT AAGGTCCTCA GAGGCGCCAT CCGCAAGCCT TGCTCCTTCA 
GCAGACTCCA CCTCCCAGTC CCAGACATTC CCGCGCCCTC AGGCACCCTC TCAGCACCCT 
CCCAGTCCCC CCAAGCGCTG CCTCAGTTAT GGGGACACCA GCCAGCTCCG GGTGCCCGAG 
GAGCCTGGGG CTGACGAGCC CGCGTCCCCT GAGGAGAGTT CGGGGCTGAG CCTCTTGCAT 
CAAGAGAGTA GGCGCCGGGC CACGCTGGCC ACGGTTCTGG AGCAGGAGCT GCCGGCGCTG 
GCGGAGAGCC TGCGCCTGGA GCAGGAACAG GGTCCCCGTC TGGAGAGGAG TCACGTGGAA 
CAGCTACTGC TCTGCCTCAG GGCGCACATC CATACTCCCA ACCGTCGGCA GCTGGCCCAG 
GAGCTGCGGG GACTCCAAGG GCAGCTTCGG GCCCAGGGCC TTGAGCCCGA GCTTCTGCAA 
GGACCGCTCT TCGCCTTCCC GGATGAGGTG AAGCAGGTCC TCCGCAGGCG CCAGATCCGC 
CCACACTGGA TGTTCGTGCT GGACTCGCTG CTCAGCCGTG CTGTGCGGGC AGCCCTGGCA 
GTGCTGGCCC CAGAGGTGGA GGAGGAGGCG GTCCTACCGA AGTCGGAAGA GGCCAGTAAT 
GCAGAGGAGT CCGAGCCCAA GCAGCAGGAG ACCCCAGCCG AGCCGAGCCC TCTCCTGGGG 
GAACCGGAGC AGGGTACCCC TTCTCTGATG GTGCAGCTGG GCCTCTTGCG AGCAGAGACC 
GATAGGCTTC GAGATGTTCT GGCTGAGAAG GAAAGGGAAT GCCAGGCCTT GGTGCAACTA 
GCCCTCCAGC GGGTCAGTGG GGAGGCCAGG ACCTGTGCCC TGGCTTCAGA GCCCCCAGCG 
GCTCTCGCGG TGGACCAGGG CCTGGTGCAG TGGTTACAGG AACTGAATGT GGATTCAGGC 
ACCATCCAGA CGCTACTGAA CCACAGAAAA AACCTCCATG CCCTACTGAC CTGTGCCACT 
AGAGATGACC TCATCTACAC AGGCATCAGG AGGCTGAATG AGGACATCCG AGGCAGGAGG 
CCAGACCCAA GGATGGATGA ATGGAGGAAA CACAAGAGGC TTCTGACATA CCTGCCCCAG 
GACCCAGCAG CAACTGGAAG AGGCCAGGTG GGAGGGCGTC AGGGTAGGGG CCAGGCCTCT 
AGCCCCAGTG GGGAGAACAA ATCACAGATG TGCCTCAAGC TCATGAGGGC CACAGGCAAA 
GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203294.1
CDS	1675..5703
Translation	MAGPSPRSGALERAGSCWQDPLAEALSRGRPLAASPGRGCARSRPLSVVYVLTREPLPEVEPETGAEAEPLPLRCLREACAQLPGPRPRPQLHSLPFGTLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDIFQKNSDCIGSYTLIPYVVTATGRVLCGDAGLLRGLADGLLQAGVGTEALLTPLVGRLAHLLEATPTDSCGYFRETIRQDIRRARERFSGLQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIDLVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVQVEGPVAPDLYCMCGRIYKDMFFSSRFQDAGHREQAYHWYRKAFDIEPSLHSGINAAVLLIADGQRFEDSEELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDLTQVALAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTLELPGEAPHGAHFWLHFLLQSCQPLKTACPQGDQCLVLVLEMNKVLLPARLEVQGTNPVSAVTLSLLEPEKQVKSPDVPSSWTFQVASISGVSVSKRDERCCFLYAPPPAQDVQLCFPSAGHCQWFCGLIQALVKSPDSGAPAEETEGAGEVLEFDYEYTESGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSVSQGGYLKIFMEEVPGGSLSSLLRSVWGPLQDNESTISFYTRQILQGLSYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGHPPFHELGSPQAAMFQVGMYKVHPPMPGSLSAEAQAFLLRTFEPDPRLRASAQALLADPFLQPGKRSRSPGSPRQALRSSEAPSASLAPSADSTSQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPGADEPASPEESSGLSLLHQESRRRATLATVLEQELPALAESLRLEQEQGPRLERSHVEQLLLCLRAHIHTPNRRQLAQELRGLQGQLRAQGLEPELLQGPLFAFPDEVKQVLRRRQIRPHWMFVLDSLLSRAVRAALAVLAPEVEEEAVLPKSEEASNAEESEPKQQETPAEPSPLLGEPEQGTPSLMVQLGLLRAETDRLRDVLAEKERECQALVQLALQRVSGEARTCALASEPPAALAVDQGLVQWLQELNVDSGTIQTLLNHRKNLHALLTCATRDDLIYTGIRRLNEDIRGRRPDPRMDEWRKHKRLLTYLPQDPAATGRGQVGGRQGRGQASSPSGENKSQMCLKLMRATGKDY

Target ORF information:

RefSeq Version	XM_005203237.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203237.3

ORF Insert Sequence:

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG TGAAGTCGCC GGACGTTCCC TCCAGCTGGA CCTTCCAGGT GGCCTCCATC 
AGCGGCGTCA GCGTCTCCAA GCGGGACGAG CGCTGCTGCT TCCTCTACGC GCCTCCTCCG 
GCTCAGGACG TCCAGCTGTG CTTCCCCAGC GCCGGGCACT GCCAGTGGTT CTGCGGCCTG 
ATCCAGGCCT TGGTGAAGAG TCCAGACTCC GGGGCGCCCG CAGAGGAGAC GGAGGGCGCT 
GGGGAGGTGT TGGAGTTTGA TTACGAGTAC ACGGAGTCGG GTGAGCGGCT GGTGCTGGGC 
AAGGGCACGT ACGGGGTGGT GTACGCTGGC CGCGATCGAC ACACTCGCGT GCGCATCGCC 
ATCAAGGAGA TCCCGGAGCG GGACAGCAGG TTCTCTCAGC CGCTGCATGA AGAGATCGCT 
CTGCACAAAC GCCTACGCCA CAAGAACATT GTGCGCTATC TGGGCTCCGT CAGCCAGGGC 
GGCTACCTCA AGATTTTCAT GGAGGAAGTG CCTGGAGGCA GCCTGTCTTC CTTGCTGCGA 
TCGGTGTGGG GACCCCTGCA GGACAACGAG AGCACCATCA GTTTCTACAC CCGCCAGATC 
CTGCAGGGAC TCAGCTACCT GCACGACAAC CACATTGTGC ATCGAGATAT CAAGGGGGAC 
AACGTGCTGA TCAACACCTT CAGCGGGTTG CTCAAGATTT CTGACTTTGG CACCTCCAAG 
CGACTAGCAG GCATCACACC CTGCACTGAG ACCTTCACAG GGACCCTACA ATATATGGCC 
CCAGAAATCA TTGACCAGGG CCCACGAGGG TATGGGAAGG CAGCAGACAT CTGGTCACTG 
GGCTGCACAG TCATCGAGAT GGCCACAGGT CACCCACCCT TCCACGAGCT AGGGAGCCCG 
CAGGCTGCCA TGTTTCAGGT GGGCATGTAC AAGGTGCATC CGCCAATGCC CGGTTCTCTG 
TCAGCTGAGG CTCAAGCCTT CCTCCTCCGA ACTTTTGAGC CCGACCCCCG TCTCCGAGCC 
AGTGCTCAAG CACTGCTGGC AGACCCCTTC CTGCAGCCCG GGAAGAGGAG CCGCAGCCCG 
GGCTCCCCCA GACAGGCTCT AAGGTCCTCA GAGGCGCCAT CCGCAAGCCT TGCTCCTTCA 
GCAGACTCCA CCTCCCAGTC CCAGACATTC CCGCGCCCTC AGGCACCCTC TCAGCACCCT 
CCCAGTCCCC CCAAGCGCTG CCTCAGTTAT GGGGACACCA GCCAGCTCCG GGTGCCCGAG 
GAGCCTGGGG CTGACGAGCC CGCGTCCCCT GAGGAGAGTT CGGGGCTGAG CCTCTTGCAT 
CAAGAGAGTA GGCGCCGGGC CACGCTGGCC ACGGTTCTGG AGCAGGAGCT GCCGGCGCTG 
GCGGAGAGCC TGCGCCTGGA GCAGGAACAG GGTCCCCGTC TGGAGAGGAG TCACGTGGAA 
CAGCTACTGC TCTGCCTCAG GGCGCACATC CATACTCCCA ACCGTCGGCA GCTGGCCCAG 
GAGCTGCGGG GACTCCAAGG GCAGCTTCGG GCCCAGGGCC TTGAGCCCGA GCTTCTGCAA 
GGACCGCTCT TCGCCTTCCC GGATGAGGTG AAGCAGGTCC TCCGCAGGCG CCAGATCCGC 
CCACACTGGA TGTTCGTGCT GGACTCGCTG CTCAGCCGTG CTGTGCGGGC AGCCCTGGCA 
GTGCTGGCCC CAGAGGTGGA GGAGGAGGCG GTCCTACCGA AGTCGGAAGA GGCCAGTAAT 
GCAGAGGAGT CCGAGCCCAA GCAGCAGGAG ACCCCAGCCG AGCCGAGCCC TCTCCTGGGG 
GAACCGGAGC AGGGTACCCC TTCTCTGATG GTGCAGCTGG GCCTCTTGCG AGCAGAGACC 
GATAGGCTTC GAGATGTTCT GGCTGAGAAG GAAAGGGAAT GCCAGGCCTT GGTGCAACTA 
GCCCTCCAGC GGGTCAGTGG GGAGGCCAGG ACCTGTGCCC TGGCTTCAGA GCCCCCAGCG 
GCTCTCGCGG TGGACCAGGG CCTGGTGCAG TGGTTACAGG AACTGAATGT GGATTCAGGC 
ACCATCCAGA CGCTACTGAA CCACAGAAAA AACCTCCATG CCCTACTGAC CTGTGCCACT 
AGAGATGACC TCATCTACAC AGGCATCAGG AGGCTGAATG AGGACATCCG AGGCAGGAGG 
CCAGACCCAA GGATGGATGA ATGGAGGAAA CACAAGAGGC TTCTGACATA CCTGCCCCAG 
GACCCAGCAG CAACTGGAAG AGGCCAGGTG GGAGGGCGTC AGGGTAGGGG CCAGGCCTCT 
AGCCCCAGTG GGGAGAACAA ATCACAGATG TGCCTCAAGC TCATGAGGGC CACAGGCAAA 
GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K6 ( XM_010802602.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800904.1 Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X2, mRNA.

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CloneID	OBa55461
Clone ID Related Accession (Same CDS sequence)	XM_010802602.2
Accession Version	XM_010802602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG TGAAGTCGCC GGACGTTCCC TCCAGCTGGA CCTTCCAGGT GGCCTCCATC 
AGCGGCGTCA GCGTCTCCAA GCGGGACGAG CGCTGCTGCT TCCTCTACGC GCCTCCTCCG 
GCTCAGGACG TCCAGCTGTG CTTCCCCAGC GCCGGGCACT GCCAGTGGTT CTGCGGCCTG 
ATCCAGGCCT TGGTGAAGAG TCCAGACTCC GGGGCGCCCG CAGAGGAGAC GGAGGGCGCT 
GGGGAGGTGT TGGAGTTTGA TTACGAGTAC ACGGAGTCGG GTGAGCGGCT GGTGCTGGGC 
AAGGGCACGT ACGGGGTGGT GTACGCTGGC CGCGATCGAC ACACTCGCGT GCGCATCGCC 
ATCAAGGAGA TCCCGGAGCG GGACAGCAGG TTCTCTCAGC CGCTGCATGA AGAGATCGCT 
CTGCACAAAC GCCTACGCCA CAAGAACATT GTGCGCTATC TGGGCTCCGT CAGCCAGGGC 
GGCTACCTCA AGATTTTCAT GGAGGAAGTG CCTGGAGGCA GCCTGTCTTC CTTGCTGCGA 
TCGGTGTGGG GACCCCTGCA GGACAACGAG AGCACCATCA GTTTCTACAC CCGCCAGATC 
CTGCAGGGAC TCAGCTACCT GCACGACAAC CACATTGTGC ATCGAGATAT CAAGGGGGAC 
AACGTGCTGA TCAACACCTT CAGCGGGTTG CTCAAGATTT CTGACTTTGG CACCTCCAAG 
CGACTAGCAG GCATCACACC CTGCACTGAG ACCTTCACAG GGACCCTACA ATATATGGCC 
CCAGAAATCA TTGACCAGGG CCCACGAGGG TATGGGAAGG CAGCAGACAT CTGGTCACTG 
GGCTGCACAG TCATCGAGAT GGCCACAGGT CACCCACCCT TCCACGAGCT AGGGAGCCCG 
CAGGCTGCCA TGTTTCAGGT GGGCATGTAC AAGGTGCATC CGCCAATGCC CGGTTCTCTG 
TCAGCTGAGG CTCAAGCCTT CCTCCTCCGA ACTTTTGAGC CCGACCCCCG TCTCCGAGCC 
AGTGCTCAAG CACTGCTGGC AGACCCCTTC CTGCAGCCCG GGAAGAGGAG CCGCAGCCCG 
GGCTCCCCCA GACAGGCTCT AAGGTCCTCA GAGGCGCCAT CCGCAAGCCT TGCTCCTTCA 
GCAGACTCCA CCTCCCAGTC CCAGACATTC CCGCGCCCTC AGGCACCCTC TCAGCACCCT 
CCCAGTCCCC CCAAGCGCTG CCTCAGTTAT GGGGACACCA GCCAGCTCCG GGTGCCCGAG 
GAGCCTGGGG CTGACGAGCC CGCGTCCCCT GAGGAGAGTT CGGGGCTGAG CCTCTTGCAT 
CAAGAGAGTA GGCGCCGGGC CACGCTGGCC ACGGTTCTGG AGCAGGAGCT GCCGGCGCTG 
GCGGAGAGCC TGCGCCTGGA GCAGGAACAG GGTCCCCGTC TGGAGAGGAG TCACGTGGAA 
CAGCTACTGC TCTGCCTCAG GGCGCACATC CATACTCCCA ACCGTCGGCA GCTGGCCCAG 
GAGCTGCGGG GACTCCAAGG GCAGCTTCGG GCCCAGGGCC TTGAGCCCGA GCTTCTGCAA 
GGACCGCTCT TCGCCTTCCC GGATGAGGTG AAGCAGGTCC TCCGCAGGCG CCAGATCCGC 
CCACACTGGA TGTTCGTGCT GGACTCGCTG CTCAGCCGTG CTGTGCGGGC AGCCCTGGCA 
GTGCTGGCCC CAGAGGTGGA GGAGGAGGCG GTCCTACCGA AGTCGGAAGA GGCCAGTAAT 
GCAGAGGAGT CCGAGCCCAA GCAGCAGGAG ACCCCAGCCG AGCCGAGCCC TCTCCTGGGG 
GAACCGGAGC AGGGTACCCC TTCTCTGATG GTGCAGCTGG GCCTCTTGCG AGCAGAGACC 
GATAGGCTTC GAGATGTTCT GGCTGAGAAG GAAAGGGAAT GCCAGGCCTT GGTGCAACTA 
GCCCTCCAGC GGGTCAGTGG GGAGGCCAGG ACCTGTGCCC TGGCTTCAGA GCCCCCAGCG 
GCTCTCGCGG TGGACCAGGG CCTGGTGCAG TGGTTACAGG AACTGAATGT GGATTCAGGC 
ACCATCCAGA CGCTACTGAA CCACAGAAAA AACCTCCATG CCCTACTGAC CTGTGCCACT 
AGAGATGACC TCATCTACAC AGGCATCAGG GGGGGGATGA TATGCCGCAT CTGGAGAGCC 
ATCTTGGCAC AGCGAACAAG ATCCACGCCA GTTACCCCTG GCCCCCAGGA GGCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800904.1
CDS	1674..5573
Translation	MAGPSPRSGALERAGSCWQDPLAEALSRGRPLAASPGRGCARSRPLSVVYVLTREPLPEVEPETGAEAEPLPLRCLREACAQLPGPRPRPQLHSLPFGTLALGDTAALDSFYNADVVVLEVSSSLAQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDIFQKNSDCIGSYTLIPYVVTATGRVLCGDAGLLRGLADGLLQAGVGTEALLTPLVGRLAHLLEATPTDSCGYFRETIRQDIRRARERFSGLQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIDLVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVQVEGPVAPDLYCMCGRIYKDMFFSSRFQDAGHREQAYHWYRKAFDIEPSLHSGINAAVLLIADGQRFEDSEELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDLTQVALAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTLELPGEAPHGAHFWLHFLLQSCQPLKTACPQGDQCLVLVLEMNKVLLPARLEVQGTNPVSAVTLSLLEPEKQVKSPDVPSSWTFQVASISGVSVSKRDERCCFLYAPPPAQDVQLCFPSAGHCQWFCGLIQALVKSPDSGAPAEETEGAGEVLEFDYEYTESGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKRLRHKNIVRYLGSVSQGGYLKIFMEEVPGGSLSSLLRSVWGPLQDNESTISFYTRQILQGLSYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGHPPFHELGSPQAAMFQVGMYKVHPPMPGSLSAEAQAFLLRTFEPDPRLRASAQALLADPFLQPGKRSRSPGSPRQALRSSEAPSASLAPSADSTSQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEEPGADEPASPEESSGLSLLHQESRRRATLATVLEQELPALAESLRLEQEQGPRLERSHVEQLLLCLRAHIHTPNRRQLAQELRGLQGQLRAQGLEPELLQGPLFAFPDEVKQVLRRRQIRPHWMFVLDSLLSRAVRAALAVLAPEVEEEAVLPKSEEASNAEESEPKQQETPAEPSPLLGEPEQGTPSLMVQLGLLRAETDRLRDVLAEKERECQALVQLALQRVSGEARTCALASEPPAALAVDQGLVQWLQELNVDSGTIQTLLNHRKNLHALLTCATRDDLIYTGIRGGMICRIWRAILAQRTRSTPVTPGPQEAE

Target ORF information:

RefSeq Version	XM_010802602.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802602.2

ORF Insert Sequence:

ATGGCAGGGC CGAGCCCGCG GTCCGGAGCT CTGGAGCGCG CCGGCAGCTG CTGGCAAGAC 
CCGCTGGCCG AGGCGCTGAG CCGGGGCCGG CCGCTCGCGG CGTCCCCGGG TCGGGGCTGT 
GCGCGAAGCC GGCCGCTCAG CGTGGTCTAC GTGCTGACCC GGGAGCCGCT GCCAGAGGTA 
GAACCCGAGA CGGGAGCCGA GGCGGAGCCG CTGCCTCTGC GCTGCCTGCG TGAGGCCTGC 
GCGCAGCTCC CCGGACCGCG GCCGCGACCT CAGTTGCACA GCCTGCCTTT TGGAACCCTG 
GCGCTGGGAG ACACCGCAGC TCTCGATTCG TTCTACAACG CGGATGTGGT GGTGCTTGAG 
GTGAGCAGCT CCCTGGCACA GCCCTCCCTG TTCTACCACC TGGGTGTGAG GGAGAGCTTC 
AGCATGACCA ACAACGTGCT CCTCTGCTCC CAGGCCGACC TCCCTGACCT GCAGGCCCTG 
CGGGAGGACA TTTTCCAGAA GAACTCGGAT TGCATTGGCA GCTACACGCT GATCCCCTAC 
GTGGTGACAG CCACCGGTCG GGTGCTATGT GGTGATGCGG GCCTCCTGAG GGGCCTGGCT 
GACGGGCTGC TCCAGGCTGG GGTGGGCACA GAGGCCCTGC TCACACCCCT GGTGGGCCGG 
CTGGCCCACC TGCTGGAAGC CACACCCACG GACTCTTGTG GCTACTTCCG GGAGACCATT 
CGGCAGGACA TCCGGAGGGC TCGGGAGCGG TTCAGTGGGC TACAGCTGCG GCAGGAGCTG 
GCTCGCCTGC AGCGGAGACT GGACAGTGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC 
CTGCTGCTCT CCTACCGGGA CGTGCAGGAC TATTCAGCCA TCATTGACCT GGTGGAGACC 
CTGCAGGCCT TGCCCACCTG TGACGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACC 
TTTGCCCTCA ACCGGAGGAA CAGGCCTGGG GACCGGGAGA AGGCACTGGC CGTGCTGCTG 
CCACTGGTAC AGGTAGAGGG CCCCGTGGCA CCTGATCTGT ACTGCATGTG TGGCCGTATC 
TACAAGGACA TGTTCTTCAG CTCTCGCTTC CAGGATGCTG GGCACCGGGA GCAGGCATAT 
CACTGGTATC GCAAGGCTTT TGACATAGAG CCCAGCCTTC ACTCGGGCAT CAATGCCGCT 
GTGCTCCTCA TTGCTGACGG GCAGCGCTTT GAGGACTCCG AGGAGCTCCG GCTCATAGGC 
ATGAAGCTGG GCTGCCTGCT GGCCCGAAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
GATGTAGGCT TCTACCTGGG AGCTCAGATC CTTGCCAATG ACCTCACTCA GGTGGCACTG 
GCCGCAGAGC AGCTGTACAA GCTCAACGCC CCCATATGGT ACCTGGTGTC CGTGATGGAA 
ACCTTCCTGC TGTATCAGCA CTTCAGACCC ACACTAGAGC TTCCTGGAGA AGCCCCCCAC 
GGCGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AGCCACTCAA GACAGCTTGT 
CCCCAAGGGG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ATAAGGTGCT GCTGCCCGCC 
AGGCTGGAGG TTCAGGGTAC AAACCCGGTG AGCGCGGTGA CCCTGAGCCT GCTGGAGCCG 
GAGAAGCAGG TGAAGTCGCC GGACGTTCCC TCCAGCTGGA CCTTCCAGGT GGCCTCCATC 
AGCGGCGTCA GCGTCTCCAA GCGGGACGAG CGCTGCTGCT TCCTCTACGC GCCTCCTCCG 
GCTCAGGACG TCCAGCTGTG CTTCCCCAGC GCCGGGCACT GCCAGTGGTT CTGCGGCCTG 
ATCCAGGCCT TGGTGAAGAG TCCAGACTCC GGGGCGCCCG CAGAGGAGAC GGAGGGCGCT 
GGGGAGGTGT TGGAGTTTGA TTACGAGTAC ACGGAGTCGG GTGAGCGGCT GGTGCTGGGC 
AAGGGCACGT ACGGGGTGGT GTACGCTGGC CGCGATCGAC ACACTCGCGT GCGCATCGCC 
ATCAAGGAGA TCCCGGAGCG GGACAGCAGG TTCTCTCAGC CGCTGCATGA AGAGATCGCT 
CTGCACAAAC GCCTACGCCA CAAGAACATT GTGCGCTATC TGGGCTCCGT CAGCCAGGGC 
GGCTACCTCA AGATTTTCAT GGAGGAAGTG CCTGGAGGCA GCCTGTCTTC CTTGCTGCGA 
TCGGTGTGGG GACCCCTGCA GGACAACGAG AGCACCATCA GTTTCTACAC CCGCCAGATC 
CTGCAGGGAC TCAGCTACCT GCACGACAAC CACATTGTGC ATCGAGATAT CAAGGGGGAC 
AACGTGCTGA TCAACACCTT CAGCGGGTTG CTCAAGATTT CTGACTTTGG CACCTCCAAG 
CGACTAGCAG GCATCACACC CTGCACTGAG ACCTTCACAG GGACCCTACA ATATATGGCC 
CCAGAAATCA TTGACCAGGG CCCACGAGGG TATGGGAAGG CAGCAGACAT CTGGTCACTG 
GGCTGCACAG TCATCGAGAT GGCCACAGGT CACCCACCCT TCCACGAGCT AGGGAGCCCG 
CAGGCTGCCA TGTTTCAGGT GGGCATGTAC AAGGTGCATC CGCCAATGCC CGGTTCTCTG 
TCAGCTGAGG CTCAAGCCTT CCTCCTCCGA ACTTTTGAGC CCGACCCCCG TCTCCGAGCC 
AGTGCTCAAG CACTGCTGGC AGACCCCTTC CTGCAGCCCG GGAAGAGGAG CCGCAGCCCG 
GGCTCCCCCA GACAGGCTCT AAGGTCCTCA GAGGCGCCAT CCGCAAGCCT TGCTCCTTCA 
GCAGACTCCA CCTCCCAGTC CCAGACATTC CCGCGCCCTC AGGCACCCTC TCAGCACCCT 
CCCAGTCCCC CCAAGCGCTG CCTCAGTTAT GGGGACACCA GCCAGCTCCG GGTGCCCGAG 
GAGCCTGGGG CTGACGAGCC CGCGTCCCCT GAGGAGAGTT CGGGGCTGAG CCTCTTGCAT 
CAAGAGAGTA GGCGCCGGGC CACGCTGGCC ACGGTTCTGG AGCAGGAGCT GCCGGCGCTG 
GCGGAGAGCC TGCGCCTGGA GCAGGAACAG GGTCCCCGTC TGGAGAGGAG TCACGTGGAA 
CAGCTACTGC TCTGCCTCAG GGCGCACATC CATACTCCCA ACCGTCGGCA GCTGGCCCAG 
GAGCTGCGGG GACTCCAAGG GCAGCTTCGG GCCCAGGGCC TTGAGCCCGA GCTTCTGCAA 
GGACCGCTCT TCGCCTTCCC GGATGAGGTG AAGCAGGTCC TCCGCAGGCG CCAGATCCGC 
CCACACTGGA TGTTCGTGCT GGACTCGCTG CTCAGCCGTG CTGTGCGGGC AGCCCTGGCA 
GTGCTGGCCC CAGAGGTGGA GGAGGAGGCG GTCCTACCGA AGTCGGAAGA GGCCAGTAAT 
GCAGAGGAGT CCGAGCCCAA GCAGCAGGAG ACCCCAGCCG AGCCGAGCCC TCTCCTGGGG 
GAACCGGAGC AGGGTACCCC TTCTCTGATG GTGCAGCTGG GCCTCTTGCG AGCAGAGACC 
GATAGGCTTC GAGATGTTCT GGCTGAGAAG GAAAGGGAAT GCCAGGCCTT GGTGCAACTA 
GCCCTCCAGC GGGTCAGTGG GGAGGCCAGG ACCTGTGCCC TGGCTTCAGA GCCCCCAGCG 
GCTCTCGCGG TGGACCAGGG CCTGGTGCAG TGGTTACAGG AACTGAATGT GGATTCAGGC 
ACCATCCAGA CGCTACTGAA CCACAGAAAA AACCTCCATG CCCTACTGAC CTGTGCCACT 
AGAGATGACC TCATCTACAC AGGCATCAGG GGGGGGATGA TATGCCGCAT CTGGAGAGCC 
ATCTTGGCAC AGCGAACAAG ATCCACGCCA GTTACCCCTG GCCCCCAGGA GGCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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1-877-436-7274

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MAP3K6 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

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