grd-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	grd-1
Entrez Gene ID	181300
Full Name	GRounDhog (hedgehog-like family)
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001029672.3	NP_001024843.1	GRounDhog (hedgehog-like family)
NM_001029672.2	NP_001024843.1	GRounDhog (hedgehog-like family)
NM_001029673.2	NP_001024844.1	GRounDhog (hedgehog-like family)

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	grd-1	NP_001024843.1
Caenorhabditis elegans (roundworm)	grd-11	NP_507923.2
Caenorhabditis elegans (roundworm)	qua-1	NP_495725.2
Caenorhabditis elegans (roundworm)	wrt-6	NP_508597.2
Caenorhabditis elegans (roundworm)	wrt-8	NP_506805.1
Caenorhabditis elegans (roundworm)	wrt-1	NP_495579.3
Caenorhabditis elegans (roundworm)	wrt-7	NP_506803.2
Caenorhabditis elegans (roundworm)	wrt-4	NP_510593.1
Caenorhabditis elegans (roundworm)	hog-1	NP_001257028.1
Caenorhabditis elegans (roundworm)	grd-2	NP_507977.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following grd-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grd-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa348527	NM_001029672.3 Latest version!	Caenorhabditis elegans GRounDhog (hedgehog-like family) (grd-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa350164	NM_001029673.2 Latest version!	Caenorhabditis elegans GRounDhog (hedgehog-like family) (grd-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OCa348527	NM_001029672.2	Caenorhabditis elegans GRounDhog (hedgehog-like family) (grd-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grd-1 ( NM_001029672.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024843.1 Caenorhabditis elegans GRounDhog (hedgehog-like family) (grd-1), mRNA.

Price & Availability↑

CloneID	OCa348527
Clone ID Related Accession (Same CDS sequence)	NM_001029672.3 , NM_001029672.2
Accession Version	NM_001029672.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	GRounDhog (hedgehog-like family)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001029672.2.

ATGAACTTGC TGATATTCTT ATTATGCTAT TTTCTTGGTT CACCTCATTA TTCAGAGGCA 
AATAAAATAA AACTGAAAAG TTCTGGCCGC TCTGCTGATA TAAACCACGA TTCTCCATGT 
ATTTGGAACA AAACTCAATC ATGGGTGCAT CCGTATCAAA GTATAACGGT AGAATGTTGC 
GATGAAGAAC TTTCCAAGCT TATCCATAAA ACTATTCTTG ACGCAGGCAA TAATGCAAAA 
CTGGGAAATC TTGCCAAGTT TATTCAACGG AGATCTCAAC TTTTCTATCA CATGAGTTTC 
GAAACTATCG TTTCAAGAGA AAACTTTGCT ATTTCAACTC ACTATCATGG AACTCATTCG 
TGTCGAGTTC ACGACAACAA TCTATATTAT TTGGTATACG AAACACCAGT TCAATATGAT 
CCTTTTAACA TGAAAACTGA AGATTATCTG GCCTCCATTG ACTCCGCTGA TCCATTGGGA 
AGTACCAAAC CCGCAAACTT ACGTGGAGAT TTTCCTGATG TACGTGAAGA TTCCACAGCT 
GGAGAAGATC TTTCTGTTCA ACCATCTATT GGCAATATTC TTCAATATCC CAACAAATTT 
GCTTGGCCAG ATCCAGATGA AGAAGTTTCC AAAAAAATCA TGCTTGCGGA ACAAGATGCA 
ATTCGTTCAA TAAATGAACT TATGGAATCA GTGACAGAGA TATCCGTTAT GAACTCTGGT 
GCAGTTGTTA ATTCAACTGA TTCTCTCAAT ATCACGGAGA TTGAATTGCC AAATCCCACT 
CCGATTCCGC GAAACTTTGC TAATTTGCGC GAAAAAGATC GACTACCCGA GAACACTCAT 
TGCGATAAAG AACGGAAAGA CGGAAATAGA TGCTGTGATG GAAGACTTGC TTCGACAATG 
AGGGATGCAA TGAGACAAAT GGCTACATCG CCAGATTTTG GACAGGGTAA AGAAGGCATT 
ATTGCTGCAG AACTTCAACA AAAAGTTCAA CAGCGCTTCC AAAAATCTTA CGAAATCATT 
GTATCCCAGT CAGACTTTGT TATTTCTACA TATACTGCTG GAGATAACTT CTGTAAATTT 
GATAACAAAG GCTTCTATAT TCTTGCTTAT GTATCACCCA AGCAATATGA TATTGATGAA 
AAAGAAGATG AGATGAAGCT TGCTGCAACT TCAAATAAAG ATCCGCTAGG AGCTAACACT 
ACGATGTTTG AAAACGAAGC ACCTTGGCAT GTCGCTTTAA AGTTGGACAC CTATGGAGAT 
AGAGCCGGAT ATCCTGTTGG GTCACACTGC ACTCAAGCAA GAACGGGGTC CAAATGTTGT 
AGTTTGATTC TATTCAATGC TATGAAGTCT GGATATGATT CTCATGTGGC AACATCAAAC 
TTTGATCCAT ATGATATTAG AAACATTTCG AAAGCGGTTC AATGGAGTGT TGAAGAAATA 
TTGCAGCACT CCGCTGAAGT GATTGTTTCG CTGGATGATT TCGCTTACGC TACATATAAC 
AATAATAGCT ACACCTGCAA ATATCGTGTT GATAAATACC ATATTCTTGC CTACACTACA 
CCGAATCATG ATCTTGATAA CTACGATGAA ATGGCAACAT CCATAATTGA TAGTGAACCA 
GAAGCCCAAA TTGTGTACCC GGCAGTCGAT AGAACAGCAA ACCAAATTCC GTTCCAAAAT 
ACACCTTCAC CATATTGGAC AACTCCAGTT CCCATCCCAA CTCAGGTTTA CATGCCAATG 
CAATACACCC AACAACCGAT GCAACCAATG TATAACCAAC AACCATTTTT TAATCAACAG 
CCCATGTTCA ATCAGCAGCC AATGTACTTG CAACCAGCTT TCAATCAAGC CCCGCTTCCA 
TATCAATACC CCGCACAACC ATCCCAGTTT AATCCATTTT TAGCTAGCTT TCGTAGATTC 
AAAAGACAGA TAGGAAGAAC ACCGTATCCA ATTCATCAAA ATGTGTACAA TGATATTGGA 
AGTGCAAAAC CTTTCAATTG TCCAGCAGAT CTGAGTGGGC TATCAGGAAT GGCTTGTTGT 
GATGGAGGCC TACAATTTGA GGCAAACAAG GTCATTGACC AAGCTAAACA AGAACCTGAT 
TTTGACAAGC ACAATACGAG AAATTTGGCA AAGTTGATGA CTCGAGCCGT GCAGAAGAGA 
TTTGGAACTA CGTTCGAGTC TGTAGTTGCC GAAGCTGATT TCTCTTGGGG AACAAACAAA 
TTCAATGGAA GAACATGCAA AATTGATTCA CAAGGGTATA ATGCTCTGAC ATATCAGTCA 
AGTTCTAAAC CACCACCACC CTCTGATTTT ATTGATATTC CAAACGATCC AACTCTTGGT 
GGTCCAACAG GCTCTAGTGG CGGTGGCGGA GGTGGAAGTA ATAGTAATTC TGGTGGTGGA 
GGTGGAAATG GTGGAGGCGG AAATGGCGGA GGAGGTAATG GTAATGGAGG AGGTGCTGGA 
GACGGAAATG GTGGAGCCGG TGCCGGTAAT GGAAACGGTG CTGGAGCTGG AAACGGAAAC 
GGAGCTGGAG CTGGAAATGG AAACGGCGCT GGCGCTGGTA ATGGAAATGG AGCAGGTGCA 
GGAAATGGAA ACGGAGCAGG AGCTGGAGAT GCGTCCGCAG CGGCCGCGGC AGCCCAAGCA 
CAGGCTGCCG CAGCAGCTCA AGCCCAGGCA GCCGCAGCAG CTGCTGCTCA ACAAGCTGCC 
GCCGCAGCAG CAGCTGCTAA TGCTCAACAA GCTGCTGCCG CCGCTGCAGC CGCAGCAAAC 
CCACTTTCTG CGTTGGTCGC TGCTACAGGA GCATGTTTCT CACTGGACAC CTGGGTCACA 
ACACCCACTG GTAAAAAGAG AATGGATCAA ATTGACATTG GCGATTATGT TCTGACAGCC 
GATCTAGAAA AGACCTATTT CACACCCATT ACTCTTTGGA TTCACCGCGA GCCGGAAAAA 
GTCCAAGAAT TCCTTACAAT TATGACGGAA TATGGGAAAA CTCTCCGAAT CACCTCGCGT 
CACTTTATGT ATCGGAACAA ATGTGGAAAA TCATATCCGC AATATATCAA AATGCTCCCT 
CATGACGGAG AAGCAATATT CGCATCAGAC CTTGAAGTTG GAGACTGTGT TGTTGTACTG 
TACAAGGGAA AATATCGCCA ACAGAAAATT GAAACTATCA CCAGATCTGT GAGAACGGGA 
ATTTATTCAC CGCTGACAAA CAATGGGAGA ATAATTGTAA ACGATATGCT AGCCTCTTGT 
TATAGTGAGA TACAGCAAAA CACACTGCAA ACAACTTTCT TCTGGGCATA TGACAAACTT 
CGTAGTGTTC TCGTTGAATT CTTTGGAGAT CTGTACAACA ACAAGATCGA ACTCCCAACC 
GGAACTACAC TCTCGAGAGA TATCATCAGT CTGATTGTTC CAATTCAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024843.1
CDS	187..3600
Translation	MNLLIFLLCYFLGSPHYSEANKIKLKSSGRSADINHDSPCIWNKTQSWVHPYQSITVECCDEELSKLIHKTILDAGNNAKLGNLAKFIQRRSQLFYHMSFETIVSRENFAISTHYHGTHSCRVHDNNLYYLVYETPVQYDPFNMKTEDYLASIDSADPLGSTKPANLRGDFPDVREDSTAGEDLSVQPSIGNILQYPNKFAWPDPDEEVSKKIMLAEQDAIRSINELMESVTEISVMNSGAVVNSTDSLNITEIELPNPTPIPRNFANLREKDRLPENTHCDKERKDGNRCCDGRLASTMRDAMRQMATSPDFGQGKEGIIAAELQQKVQQRFQKSYEIIVSQSDFVISTYTAGDNFCKFDNKGFYILAYVSPKQYDIDEKEDEMKLAATSNKDPLGANTTMFENEAPWHVALKLDTYGDRAGYPVGSHCTQARTGSKCCSLILFNAMKSGYDSHVATSNFDPYDIRNISKAVQWSVEEILQHSAEVIVSLDDFAYATYNNNSYTCKYRVDKYHILAYTTPNHDLDNYDEMATSIIDSEPEAQIVYPAVDRTANQIPFQNTPSPYWTTPVPIPTQVYMPMQYTQQPMQPMYNQQPFFNQQPMFNQQPMYLQPAFNQAPLPYQYPAQPSQFNPFLASFRRFKRQIGRTPYPIHQNVYNDIGSAKPFNCPADLSGLSGMACCDGGLQFEANKVIDQAKQEPDFDKHNTRNLAKLMTRAVQKRFGTTFESVVAEADFSWGTNKFNGRTCKIDSQGYNALTYQSSSKPPPPSDFIDIPNDPTLGGPTGSSGGGGGGSNSNSGGGGGNGGGGNGGGGNGNGGGAGDGNGGAGAGNGNGAGAGNGNGAGAGNGNGAGAGNGNGAGAGNGNGAGAGDASAAAAAAQAQAAAAAQAQAAAAAAAQQAAAAAAAANAQQAAAAAAAAANPLSALVAATGACFSLDTWVTTPTGKKRMDQIDIGDYVLTADLEKTYFTPITLWIHREPEKVQEFLTIMTEYGKTLRITSRHFMYRNKCGKSYPQYIKMLPHDGEAIFASDLEVGDCVVVLYKGKYRQQKIETITRSVRTGIYSPLTNNGRIIVNDMLASCYSEIQQNTLQTTFFWAYDKLRSVLVEFFGDLYNNKIELPTGTTLSRDIISLIVPIQK

Target ORF information:

RefSeq Version	NM_001029672.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans GRounDhog (hedgehog-like family) (grd-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029672.3

ORF Insert Sequence:

ATGAACTTGC TGATATTCTT ATTATGCTAT TTTCTTGGTT CACCTCATTA TTCAGAGGCA 
AATAAAATAA AACTGAAAAG TTCTGGCCGC TCTGCTGATA TAAACCACGA TTCTCCATGT 
ATTTGGAACA AAACTCAATC ATGGGTGCAT CCGTATCAAA GTATAACGGT AGAATGTTGC 
GATGAAGAAC TTTCCAAGCT TATCCATAAA ACTATTCTTG ACGCAGGCAA TAATGCAAAA 
CTGGGAAATC TTGCCAAGTT TATTCAACGG AGATCTCAAC TTTTCTATCA CATGAGTTTC 
GAAACTATCG TTTCAAGAGA AAACTTTGCT ATTTCAACTC ACTATCATGG AACTCATTCG 
TGTCGAGTTC ACGACAACAA TCTATATTAT TTGGTATACG AAACACCAGT TCAATATGAT 
CCTTTTAACA TGAAAACTGA AGATTATCTG GCCTCCATTG ACTCCGCTGA TCCATTGGGA 
AGTACCAAAC CCGCAAACTT ACGTGGAGAT TTTCCTGATG TACGTGAAGA TTCCACAGCT 
GGAGAAGATC TTTCTGTTCA ACCATCTATT GGCAATATTC TTCAATATCC CAACAAATTT 
GCTTGGCCAG ATCCAGATGA AGAAGTTTCC AAAAAAATCA TGCTTGCGGA ACAAGATGCA 
ATTCGTTCAA TAAATGAACT TATGGAATCA GTGACAGAGA TATCCGTTAT GAACTCTGGT 
GCAGTTGTTA ATTCAACTGA TTCTCTCAAT ATCACGGAGA TTGAATTGCC AAATCCCACT 
CCGATTCCGC GAAACTTTGC TAATTTGCGC GAAAAAGATC GACTACCCGA GAACACTCAT 
TGCGATAAAG AACGGAAAGA CGGAAATAGA TGCTGTGATG GAAGACTTGC TTCGACAATG 
AGGGATGCAA TGAGACAAAT GGCTACATCG CCAGATTTTG GACAGGGTAA AGAAGGCATT 
ATTGCTGCAG AACTTCAACA AAAAGTTCAA CAGCGCTTCC AAAAATCTTA CGAAATCATT 
GTATCCCAGT CAGACTTTGT TATTTCTACA TATACTGCTG GAGATAACTT CTGTAAATTT 
GATAACAAAG GCTTCTATAT TCTTGCTTAT GTATCACCCA AGCAATATGA TATTGATGAA 
AAAGAAGATG AGATGAAGCT TGCTGCAACT TCAAATAAAG ATCCGCTAGG AGCTAACACT 
ACGATGTTTG AAAACGAAGC ACCTTGGCAT GTCGCTTTAA AGTTGGACAC CTATGGAGAT 
AGAGCCGGAT ATCCTGTTGG GTCACACTGC ACTCAAGCAA GAACGGGGTC CAAATGTTGT 
AGTTTGATTC TATTCAATGC TATGAAGTCT GGATATGATT CTCATGTGGC AACATCAAAC 
TTTGATCCAT ATGATATTAG AAACATTTCG AAAGCGGTTC AATGGAGTGT TGAAGAAATA 
TTGCAGCACT CCGCTGAAGT GATTGTTTCG CTGGATGATT TCGCTTACGC TACATATAAC 
AATAATAGCT ACACCTGCAA ATATCGTGTT GATAAATACC ATATTCTTGC CTACACTACA 
CCGAATCATG ATCTTGATAA CTACGATGAA ATGGCAACAT CCATAATTGA TAGTGAACCA 
GAAGCCCAAA TTGTGTACCC GGCAGTCGAT AGAACAGCAA ACCAAATTCC GTTCCAAAAT 
ACACCTTCAC CATATTGGAC AACTCCAGTT CCCATCCCAA CTCAGGTTTA CATGCCAATG 
CAATACACCC AACAACCGAT GCAACCAATG TATAACCAAC AACCATTTTT TAATCAACAG 
CCCATGTTCA ATCAGCAGCC AATGTACTTG CAACCAGCTT TCAATCAAGC CCCGCTTCCA 
TATCAATACC CCGCACAACC ATCCCAGTTT AATCCATTTT TAGCTAGCTT TCGTAGATTC 
AAAAGACAGA TAGGAAGAAC ACCGTATCCA ATTCATCAAA ATGTGTACAA TGATATTGGA 
AGTGCAAAAC CTTTCAATTG TCCAGCAGAT CTGAGTGGGC TATCAGGAAT GGCTTGTTGT 
GATGGAGGCC TACAATTTGA GGCAAACAAG GTCATTGACC AAGCTAAACA AGAACCTGAT 
TTTGACAAGC ACAATACGAG AAATTTGGCA AAGTTGATGA CTCGAGCCGT GCAGAAGAGA 
TTTGGAACTA CGTTCGAGTC TGTAGTTGCC GAAGCTGATT TCTCTTGGGG AACAAACAAA 
TTCAATGGAA GAACATGCAA AATTGATTCA CAAGGGTATA ATGCTCTGAC ATATCAGTCA 
AGTTCTAAAC CACCACCACC CTCTGATTTT ATTGATATTC CAAACGATCC AACTCTTGGT 
GGTCCAACAG GCTCTAGTGG CGGTGGCGGA GGTGGAAGTA ATAGTAATTC TGGTGGTGGA 
GGTGGAAATG GTGGAGGCGG AAATGGCGGA GGAGGTAATG GTAATGGAGG AGGTGCTGGA 
GACGGAAATG GTGGAGCCGG TGCCGGTAAT GGAAACGGTG CTGGAGCTGG AAACGGAAAC 
GGAGCTGGAG CTGGAAATGG AAACGGCGCT GGCGCTGGTA ATGGAAATGG AGCAGGTGCA 
GGAAATGGAA ACGGAGCAGG AGCTGGAGAT GCGTCCGCAG CGGCCGCGGC AGCCCAAGCA 
CAGGCTGCCG CAGCAGCTCA AGCCCAGGCA GCCGCAGCAG CTGCTGCTCA ACAAGCTGCC 
GCCGCAGCAG CAGCTGCTAA TGCTCAACAA GCTGCTGCCG CCGCTGCAGC CGCAGCAAAC 
CCACTTTCTG CGTTGGTCGC TGCTACAGGA GCATGTTTCT CACTGGACAC CTGGGTCACA 
ACACCCACTG GTAAAAAGAG AATGGATCAA ATTGACATTG GCGATTATGT TCTGACAGCC 
GATCTAGAAA AGACCTATTT CACACCCATT ACTCTTTGGA TTCACCGCGA GCCGGAAAAA 
GTCCAAGAAT TCCTTACAAT TATGACGGAA TATGGGAAAA CTCTCCGAAT CACCTCGCGT 
CACTTTATGT ATCGGAACAA ATGTGGAAAA TCATATCCGC AATATATCAA AATGCTCCCT 
CATGACGGAG AAGCAATATT CGCATCAGAC CTTGAAGTTG GAGACTGTGT TGTTGTACTG 
TACAAGGGAA AATATCGCCA ACAGAAAATT GAAACTATCA CCAGATCTGT GAGAACGGGA 
ATTTATTCAC CGCTGACAAA CAATGGGAGA ATAATTGTAA ACGATATGCT AGCCTCTTGT 
TATAGTGAGA TACAGCAAAA CACACTGCAA ACAACTTTCT TCTGGGCATA TGACAAACTT 
CGTAGTGTTC TCGTTGAATT CTTTGGAGAT CTGTACAACA ACAAGATCGA ACTCCCAACC 
GGAACTACAC TCTCGAGAGA TATCATCAGT CTGATTGTTC CAATTCAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grd-1 ( NM_001029672.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024843.1 Caenorhabditis elegans GRounDhog (hedgehog-like family) (grd-1), partial mRNA.

Price & Availability↑

CloneID	OCa348527
Clone ID Related Accession (Same CDS sequence)	NM_001029672.3 , NM_001029672.2
Accession Version	NM_001029672.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	GRounDhog (hedgehog-like family)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 27, 2008 this sequence version replaced NM_001029672.1. COMPLETENESS: incomplete on both ends.

ATGAACTTGC TGATATTCTT ATTATGCTAT TTTCTTGGTT CACCTCATTA TTCAGAGGCA 
AATAAAATAA AACTGAAAAG TTCTGGCCGC TCTGCTGATA TAAACCACGA TTCTCCATGT 
ATTTGGAACA AAACTCAATC ATGGGTGCAT CCGTATCAAA GTATAACGGT AGAATGTTGC 
GATGAAGAAC TTTCCAAGCT TATCCATAAA ACTATTCTTG ACGCAGGCAA TAATGCAAAA 
CTGGGAAATC TTGCCAAGTT TATTCAACGG AGATCTCAAC TTTTCTATCA CATGAGTTTC 
GAAACTATCG TTTCAAGAGA AAACTTTGCT ATTTCAACTC ACTATCATGG AACTCATTCG 
TGTCGAGTTC ACGACAACAA TCTATATTAT TTGGTATACG AAACACCAGT TCAATATGAT 
CCTTTTAACA TGAAAACTGA AGATTATCTG GCCTCCATTG ACTCCGCTGA TCCATTGGGA 
AGTACCAAAC CCGCAAACTT ACGTGGAGAT TTTCCTGATG TACGTGAAGA TTCCACAGCT 
GGAGAAGATC TTTCTGTTCA ACCATCTATT GGCAATATTC TTCAATATCC CAACAAATTT 
GCTTGGCCAG ATCCAGATGA AGAAGTTTCC AAAAAAATCA TGCTTGCGGA ACAAGATGCA 
ATTCGTTCAA TAAATGAACT TATGGAATCA GTGACAGAGA TATCCGTTAT GAACTCTGGT 
GCAGTTGTTA ATTCAACTGA TTCTCTCAAT ATCACGGAGA TTGAATTGCC AAATCCCACT 
CCGATTCCGC GAAACTTTGC TAATTTGCGC GAAAAAGATC GACTACCCGA GAACACTCAT 
TGCGATAAAG AACGGAAAGA CGGAAATAGA TGCTGTGATG GAAGACTTGC TTCGACAATG 
AGGGATGCAA TGAGACAAAT GGCTACATCG CCAGATTTTG GACAGGGTAA AGAAGGCATT 
ATTGCTGCAG AACTTCAACA AAAAGTTCAA CAGCGCTTCC AAAAATCTTA CGAAATCATT 
GTATCCCAGT CAGACTTTGT TATTTCTACA TATACTGCTG GAGATAACTT CTGTAAATTT 
GATAACAAAG GCTTCTATAT TCTTGCTTAT GTATCACCCA AGCAATATGA TATTGATGAA 
AAAGAAGATG AGATGAAGCT TGCTGCAACT TCAAATAAAG ATCCGCTAGG AGCTAACACT 
ACGATGTTTG AAAACGAAGC ACCTTGGCAT GTCGCTTTAA AGTTGGACAC CTATGGAGAT 
AGAGCCGGAT ATCCTGTTGG GTCACACTGC ACTCAAGCAA GAACGGGGTC CAAATGTTGT 
AGTTTGATTC TATTCAATGC TATGAAGTCT GGATATGATT CTCATGTGGC AACATCAAAC 
TTTGATCCAT ATGATATTAG AAACATTTCG AAAGCGGTTC AATGGAGTGT TGAAGAAATA 
TTGCAGCACT CCGCTGAAGT GATTGTTTCG CTGGATGATT TCGCTTACGC TACATATAAC 
AATAATAGCT ACACCTGCAA ATATCGTGTT GATAAATACC ATATTCTTGC CTACACTACA 
CCGAATCATG ATCTTGATAA CTACGATGAA ATGGCAACAT CCATAATTGA TAGTGAACCA 
GAAGCCCAAA TTGTGTACCC GGCAGTCGAT AGAACAGCAA ACCAAATTCC GTTCCAAAAT 
ACACCTTCAC CATATTGGAC AACTCCAGTT CCCATCCCAA CTCAGGTTTA CATGCCAATG 
CAATACACCC AACAACCGAT GCAACCAATG TATAACCAAC AACCATTTTT TAATCAACAG 
CCCATGTTCA ATCAGCAGCC AATGTACTTG CAACCAGCTT TCAATCAAGC CCCGCTTCCA 
TATCAATACC CCGCACAACC ATCCCAGTTT AATCCATTTT TAGCTAGCTT TCGTAGATTC 
AAAAGACAGA TAGGAAGAAC ACCGTATCCA ATTCATCAAA ATGTGTACAA TGATATTGGA 
AGTGCAAAAC CTTTCAATTG TCCAGCAGAT CTGAGTGGGC TATCAGGAAT GGCTTGTTGT 
GATGGAGGCC TACAATTTGA GGCAAACAAG GTCATTGACC AAGCTAAACA AGAACCTGAT 
TTTGACAAGC ACAATACGAG AAATTTGGCA AAGTTGATGA CTCGAGCCGT GCAGAAGAGA 
TTTGGAACTA CGTTCGAGTC TGTAGTTGCC GAAGCTGATT TCTCTTGGGG AACAAACAAA 
TTCAATGGAA GAACATGCAA AATTGATTCA CAAGGGTATA ATGCTCTGAC ATATCAGTCA 
AGTTCTAAAC CACCACCACC CTCTGATTTT ATTGATATTC CAAACGATCC AACTCTTGGT 
GGTCCAACAG GCTCTAGTGG CGGTGGCGGA GGTGGAAGTA ATAGTAATTC TGGTGGTGGA 
GGTGGAAATG GTGGAGGCGG AAATGGCGGA GGAGGTAATG GTAATGGAGG AGGTGCTGGA 
GACGGAAATG GTGGAGCCGG TGCCGGTAAT GGAAACGGTG CTGGAGCTGG AAACGGAAAC 
GGAGCTGGAG CTGGAAATGG AAACGGCGCT GGCGCTGGTA ATGGAAATGG AGCAGGTGCA 
GGAAATGGAA ACGGAGCAGG AGCTGGAGAT GCGTCCGCAG CGGCCGCGGC AGCCCAAGCA 
CAGGCTGCCG CAGCAGCTCA AGCCCAGGCA GCCGCAGCAG CTGCTGCTCA ACAAGCTGCC 
GCCGCAGCAG CAGCTGCTAA TGCTCAACAA GCTGCTGCCG CCGCTGCAGC CGCAGCAAAC 
CCACTTTCTG CGTTGGTCGC TGCTACAGGA GCATGTTTCT CACTGGACAC CTGGGTCACA 
ACACCCACTG GTAAAAAGAG AATGGATCAA ATTGACATTG GCGATTATGT TCTGACAGCC 
GATCTAGAAA AGACCTATTT CACACCCATT ACTCTTTGGA TTCACCGCGA GCCGGAAAAA 
GTCCAAGAAT TCCTTACAAT TATGACGGAA TATGGGAAAA CTCTCCGAAT CACCTCGCGT 
CACTTTATGT ATCGGAACAA ATGTGGAAAA TCATATCCGC AATATATCAA AATGCTCCCT 
CATGACGGAG AAGCAATATT CGCATCAGAC CTTGAAGTTG GAGACTGTGT TGTTGTACTG 
TACAAGGGAA AATATCGCCA ACAGAAAATT GAAACTATCA CCAGATCTGT GAGAACGGGA 
ATTTATTCAC CGCTGACAAA CAATGGGAGA ATAATTGTAA ACGATATGCT AGCCTCTTGT 
TATAGTGAGA TACAGCAAAA CACACTGCAA ACAACTTTCT TCTGGGCATA TGACAAACTT 
CGTAGTGTTC TCGTTGAATT CTTTGGAGAT CTGTACAACA ACAAGATCGA ACTCCCAACC 
GGAACTACAC TCTCGAGAGA TATCATCAGT CTGATTGTTC CAATTCAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024843.1
CDS	1..3414
Translation	MNLLIFLLCYFLGSPHYSEANKIKLKSSGRSADINHDSPCIWNKTQSWVHPYQSITVECCDEELSKLIHKTILDAGNNAKLGNLAKFIQRRSQLFYHMSFETIVSRENFAISTHYHGTHSCRVHDNNLYYLVYETPVQYDPFNMKTEDYLASIDSADPLGSTKPANLRGDFPDVREDSTAGEDLSVQPSIGNILQYPNKFAWPDPDEEVSKKIMLAEQDAIRSINELMESVTEISVMNSGAVVNSTDSLNITEIELPNPTPIPRNFANLREKDRLPENTHCDKERKDGNRCCDGRLASTMRDAMRQMATSPDFGQGKEGIIAAELQQKVQQRFQKSYEIIVSQSDFVISTYTAGDNFCKFDNKGFYILAYVSPKQYDIDEKEDEMKLAATSNKDPLGANTTMFENEAPWHVALKLDTYGDRAGYPVGSHCTQARTGSKCCSLILFNAMKSGYDSHVATSNFDPYDIRNISKAVQWSVEEILQHSAEVIVSLDDFAYATYNNNSYTCKYRVDKYHILAYTTPNHDLDNYDEMATSIIDSEPEAQIVYPAVDRTANQIPFQNTPSPYWTTPVPIPTQVYMPMQYTQQPMQPMYNQQPFFNQQPMFNQQPMYLQPAFNQAPLPYQYPAQPSQFNPFLASFRRFKRQIGRTPYPIHQNVYNDIGSAKPFNCPADLSGLSGMACCDGGLQFEANKVIDQAKQEPDFDKHNTRNLAKLMTRAVQKRFGTTFESVVAEADFSWGTNKFNGRTCKIDSQGYNALTYQSSSKPPPPSDFIDIPNDPTLGGPTGSSGGGGGGSNSNSGGGGGNGGGGNGGGGNGNGGGAGDGNGGAGAGNGNGAGAGNGNGAGAGNGNGAGAGNGNGAGAGNGNGAGAGDASAAAAAAQAQAAAAAQAQAAAAAAAQQAAAAAAAANAQQAAAAAAAAANPLSALVAATGACFSLDTWVTTPTGKKRMDQIDIGDYVLTADLEKTYFTPITLWIHREPEKVQEFLTIMTEYGKTLRITSRHFMYRNKCGKSYPQYIKMLPHDGEAIFASDLEVGDCVVVLYKGKYRQQKIETITRSVRTGIYSPLTNNGRIIVNDMLASCYSEIQQNTLQTTFFWAYDKLRSVLVEFFGDLYNNKIELPTGTTLSRDIISLIVPIQK

Target ORF information:

RefSeq Version	NM_001029672.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans GRounDhog (hedgehog-like family) (grd-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029672.2

ORF Insert Sequence:

ATGAACTTGC TGATATTCTT ATTATGCTAT TTTCTTGGTT CACCTCATTA TTCAGAGGCA 
AATAAAATAA AACTGAAAAG TTCTGGCCGC TCTGCTGATA TAAACCACGA TTCTCCATGT 
ATTTGGAACA AAACTCAATC ATGGGTGCAT CCGTATCAAA GTATAACGGT AGAATGTTGC 
GATGAAGAAC TTTCCAAGCT TATCCATAAA ACTATTCTTG ACGCAGGCAA TAATGCAAAA 
CTGGGAAATC TTGCCAAGTT TATTCAACGG AGATCTCAAC TTTTCTATCA CATGAGTTTC 
GAAACTATCG TTTCAAGAGA AAACTTTGCT ATTTCAACTC ACTATCATGG AACTCATTCG 
TGTCGAGTTC ACGACAACAA TCTATATTAT TTGGTATACG AAACACCAGT TCAATATGAT 
CCTTTTAACA TGAAAACTGA AGATTATCTG GCCTCCATTG ACTCCGCTGA TCCATTGGGA 
AGTACCAAAC CCGCAAACTT ACGTGGAGAT TTTCCTGATG TACGTGAAGA TTCCACAGCT 
GGAGAAGATC TTTCTGTTCA ACCATCTATT GGCAATATTC TTCAATATCC CAACAAATTT 
GCTTGGCCAG ATCCAGATGA AGAAGTTTCC AAAAAAATCA TGCTTGCGGA ACAAGATGCA 
ATTCGTTCAA TAAATGAACT TATGGAATCA GTGACAGAGA TATCCGTTAT GAACTCTGGT 
GCAGTTGTTA ATTCAACTGA TTCTCTCAAT ATCACGGAGA TTGAATTGCC AAATCCCACT 
CCGATTCCGC GAAACTTTGC TAATTTGCGC GAAAAAGATC GACTACCCGA GAACACTCAT 
TGCGATAAAG AACGGAAAGA CGGAAATAGA TGCTGTGATG GAAGACTTGC TTCGACAATG 
AGGGATGCAA TGAGACAAAT GGCTACATCG CCAGATTTTG GACAGGGTAA AGAAGGCATT 
ATTGCTGCAG AACTTCAACA AAAAGTTCAA CAGCGCTTCC AAAAATCTTA CGAAATCATT 
GTATCCCAGT CAGACTTTGT TATTTCTACA TATACTGCTG GAGATAACTT CTGTAAATTT 
GATAACAAAG GCTTCTATAT TCTTGCTTAT GTATCACCCA AGCAATATGA TATTGATGAA 
AAAGAAGATG AGATGAAGCT TGCTGCAACT TCAAATAAAG ATCCGCTAGG AGCTAACACT 
ACGATGTTTG AAAACGAAGC ACCTTGGCAT GTCGCTTTAA AGTTGGACAC CTATGGAGAT 
AGAGCCGGAT ATCCTGTTGG GTCACACTGC ACTCAAGCAA GAACGGGGTC CAAATGTTGT 
AGTTTGATTC TATTCAATGC TATGAAGTCT GGATATGATT CTCATGTGGC AACATCAAAC 
TTTGATCCAT ATGATATTAG AAACATTTCG AAAGCGGTTC AATGGAGTGT TGAAGAAATA 
TTGCAGCACT CCGCTGAAGT GATTGTTTCG CTGGATGATT TCGCTTACGC TACATATAAC 
AATAATAGCT ACACCTGCAA ATATCGTGTT GATAAATACC ATATTCTTGC CTACACTACA 
CCGAATCATG ATCTTGATAA CTACGATGAA ATGGCAACAT CCATAATTGA TAGTGAACCA 
GAAGCCCAAA TTGTGTACCC GGCAGTCGAT AGAACAGCAA ACCAAATTCC GTTCCAAAAT 
ACACCTTCAC CATATTGGAC AACTCCAGTT CCCATCCCAA CTCAGGTTTA CATGCCAATG 
CAATACACCC AACAACCGAT GCAACCAATG TATAACCAAC AACCATTTTT TAATCAACAG 
CCCATGTTCA ATCAGCAGCC AATGTACTTG CAACCAGCTT TCAATCAAGC CCCGCTTCCA 
TATCAATACC CCGCACAACC ATCCCAGTTT AATCCATTTT TAGCTAGCTT TCGTAGATTC 
AAAAGACAGA TAGGAAGAAC ACCGTATCCA ATTCATCAAA ATGTGTACAA TGATATTGGA 
AGTGCAAAAC CTTTCAATTG TCCAGCAGAT CTGAGTGGGC TATCAGGAAT GGCTTGTTGT 
GATGGAGGCC TACAATTTGA GGCAAACAAG GTCATTGACC AAGCTAAACA AGAACCTGAT 
TTTGACAAGC ACAATACGAG AAATTTGGCA AAGTTGATGA CTCGAGCCGT GCAGAAGAGA 
TTTGGAACTA CGTTCGAGTC TGTAGTTGCC GAAGCTGATT TCTCTTGGGG AACAAACAAA 
TTCAATGGAA GAACATGCAA AATTGATTCA CAAGGGTATA ATGCTCTGAC ATATCAGTCA 
AGTTCTAAAC CACCACCACC CTCTGATTTT ATTGATATTC CAAACGATCC AACTCTTGGT 
GGTCCAACAG GCTCTAGTGG CGGTGGCGGA GGTGGAAGTA ATAGTAATTC TGGTGGTGGA 
GGTGGAAATG GTGGAGGCGG AAATGGCGGA GGAGGTAATG GTAATGGAGG AGGTGCTGGA 
GACGGAAATG GTGGAGCCGG TGCCGGTAAT GGAAACGGTG CTGGAGCTGG AAACGGAAAC 
GGAGCTGGAG CTGGAAATGG AAACGGCGCT GGCGCTGGTA ATGGAAATGG AGCAGGTGCA 
GGAAATGGAA ACGGAGCAGG AGCTGGAGAT GCGTCCGCAG CGGCCGCGGC AGCCCAAGCA 
CAGGCTGCCG CAGCAGCTCA AGCCCAGGCA GCCGCAGCAG CTGCTGCTCA ACAAGCTGCC 
GCCGCAGCAG CAGCTGCTAA TGCTCAACAA GCTGCTGCCG CCGCTGCAGC CGCAGCAAAC 
CCACTTTCTG CGTTGGTCGC TGCTACAGGA GCATGTTTCT CACTGGACAC CTGGGTCACA 
ACACCCACTG GTAAAAAGAG AATGGATCAA ATTGACATTG GCGATTATGT TCTGACAGCC 
GATCTAGAAA AGACCTATTT CACACCCATT ACTCTTTGGA TTCACCGCGA GCCGGAAAAA 
GTCCAAGAAT TCCTTACAAT TATGACGGAA TATGGGAAAA CTCTCCGAAT CACCTCGCGT 
CACTTTATGT ATCGGAACAA ATGTGGAAAA TCATATCCGC AATATATCAA AATGCTCCCT 
CATGACGGAG AAGCAATATT CGCATCAGAC CTTGAAGTTG GAGACTGTGT TGTTGTACTG 
TACAAGGGAA AATATCGCCA ACAGAAAATT GAAACTATCA CCAGATCTGT GAGAACGGGA 
ATTTATTCAC CGCTGACAAA CAATGGGAGA ATAATTGTAA ACGATATGCT AGCCTCTTGT 
TATAGTGAGA TACAGCAAAA CACACTGCAA ACAACTTTCT TCTGGGCATA TGACAAACTT 
CGTAGTGTTC TCGTTGAATT CTTTGGAGAT CTGTACAACA ACAAGATCGA ACTCCCAACC 
GGAACTACAC TCTCGAGAGA TATCATCAGT CTGATTGTTC CAATTCAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grd-1 ( NM_001029673.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024844.1 Caenorhabditis elegans GRounDhog (hedgehog-like family) (grd-1), partial mRNA.

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CloneID	OCa350164
Clone ID Related Accession (Same CDS sequence)	NM_001029673.2
Accession Version	NM_001029673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	GRounDhog (hedgehog-like family)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001029673.1. COMPLETENESS: incomplete on both ends.

ATGAACTTGC TGATATTCTT ATTATGCTAT TTTCTTGGTT CACCTCATTA TTCAGAGGCA 
AATAAAATAA AACTGAAAAG TTCTGGCCGC TCTGCTGATA TAAACCACGA TTCTCCATGT 
ATTTGGAACA AAACTCAATC ATGGGTGCAT CCGTATCAAA GTATAACGGT AGAATGTTGC 
GATGAAGAAC TTTCCAAGCT TATCCATAAA ACTATTCTTG ACGCAGGCAA TAATGCAAAA 
CTGGGAAATC TTGCCAAGTT TATTCAACGG AGATCTCAAC TTTTCTATCA CATGAGTTTC 
GAAACTATCG TTTCAAGAGA AAACTTTGCT ATTTCAACTC ACTATCATGG AACTCATTCG 
TGTCGAGTTC ACGACAACAA TCTATATTAT TTGGTATACG AAACACCAGT TCAATATGAT 
CCTTTTAACA TGAAAACTGA AGATTATCTG GCCTCCATTG ACTCCGCTGA TCCATTGGGA 
AGTACCAAAC CCGCAAACTT ACGTGGAGAT TTTCCTGATG TACGTGAAGA TTCCACAGCT 
GGAGAAGATC TTTCTGTTCA ACCATCTATT GGCAATATTC TTCAATATCC CAACAAATTT 
GCTTGGCCAG ATCCAGATGA AGAAGTTTCC AAAAAAATCA TGCTTGCGGA ACAAGATGCA 
ATTCGTTCAA TAAATGAACT TATGGAATCA GTGACAGAGA TATCCGTTAT GAACTCTGGT 
GCAGTTGTTA ATTCAACTGA TTCTCTCAAT ATCACGGAGA TTGAATTGCC AAATCCCACT 
CCGATTCCGC GAAACTTTGC TAATTTGCGC GAAAAAGATC GACTACCCGA GAACACTCAT 
TGCGATAAAG AACGGAAAGA CGGAAATAGA TGCTGTGATG GAAGACTTGC TTCGACAATG 
AGGGATGCAA TGAGACAAAT GGCTACATCG CCAGATTTTG GACAGGGTAA AGAAGGCATT 
ATTGCTGCAG AACTTCAACA AAAAGTTCAA CAGCGCTTCC AAAAATCTTA CGAAATCATT 
GTATCCCAGT CAGACTTTGT TATTTCTACA TATACTGCTG GAGATAACTT CTGTAAATTT 
GATAACAAAG GCTTCTATAT TCTTGCTTAT GTATCACCCA AGCAATATGA TATTGATGAA 
AAAGAAGATG AGATGAAGCT TGCTGCAACT TCAAATAAAG ATCCGCTAGG AGCTAACACT 
ACGATGTTTG AAAACGAAGC ACCTTGGCAT GTCGCTTTAA AGTTGGACAC CTATGGAGAT 
AGAGCCGGAT ATCCTGTTGG GTCACACTGC ACTCAAGCAA GAACGGGGTC CAAATGTTGT 
AGTTTGATTC TATTCAATGC TATGAAGTCT GGATATGATT CTCATGTGGC AACATCAAAC 
TTTGATCCAT ATGATATTAG AAACATTTCG AAAGCGGTTC AATGGAGTGT TGAAGAAATA 
TTGCAGCACT CCGCTGAAGT GATTGTTTCG CTGGATGATT TCGCTTACGC TACATATAAC 
AATAATAGCT ACACCTGCAA ATATCGTGTT GATAAATACC ATATTCTTGC CTACACTACA 
CCGAATCATG ATCTTGATAA CTACGATGAA ATGGCAACAT CCATAATTGA TAGTGAACCA 
GAAGCCCAAA TTGTGTACCC GGCAGTCGAT AGAACAGCAA ACCAAATTCC GTTCCAAAAT 
ACACCTTCAC CATATTGGAC AACTCCAGTT CCCATCCCAA CTCAGGTTTA CATGCCAATG 
CAATACACCC AACAACCGAT GCAACCAATG TATAACCAAC AACCATTTTT TAATCAACAG 
CCCATGTTCA ATCAGCAGCC AATGTACTTG CAACCAGCTT TCAATCAAGC CCCGCTTCCA 
TATCAATACC CCGCACAACC ATCCCAGTTT AATCCATTTT TAGCTAGCTT TCGTAGATTC 
AAAAGACAGA TAGGAAGAAC ACCGTATCCA ATTCATCAAA ATGTGTACAA TGATATTGGA 
AGTGCAAAAC CTTTCAATTG TCCAGCAGAT CTGAGTGGGC TATCAGGAAT GGCTTGTTGT 
GATGGAGGCC TACAATTTGA GGCAAACAAG GTCATTGACC AAGCTAAACA AGAACCTGAT 
TTTGACAAGC ACAATACGAG AAATTTGGCA AAGTTGATGA CTCGAGCCGT GCAGAAGAGA 
TTTGGAACTA CGTTCGAGTC TGTAGTTGCC GAAGCTGATT TCTCTTGGGG AACAAACAAA 
TTCAATGGAA GAACATGCAA AATTGATTCA CAAGGGTATA ATGCTCTGAC ATATCAGTCA 
AGTTCTAAAC CACCACCACC CTCTGATTTT ATTGATATTC CAAACGATCC AACTCTTGGT 
GGTCCAACAG GCTCTAGTGG CGGTGGCGGA GGAGGAGGAA ATGGTGGAAG TGGTGGAGGC 
GGAGGAGGCG GCGGCGGAGG TTCAGGAGGA AGTGGCGGAG GTGGAAGTAA TAGTAATTCT 
GGTGGTGGAG GTGGAAATGG TGGAGGCGGA AATGGCGGAG GAGGTAATGG TAATGGAGGA 
GGTGCTGGAG ACGGAAATGG TGGAGCCGGT GCCGGTAATG GAAACGGTGC TGGAGCTGGA 
AACGGAAACG GAGCTGGAGC TGGAAATGGA AACGGCGCTG GCGCTGGTAA TGGAAATGGA 
GCAGGTGCAG GAAATGGAAA CGGAGCAGGA GCTGGAGATG CGTCCGCAGC GGCCGCGGCA 
GCCCAAGCAC AGGCTGCCGC AGCAGCTCAA GCCCAGGCAG CCGCAGCAGC TGCTGCTCAA 
CAAGCTGCCG CCGCAGCAGC AGCTGCTAAT GCTCAACAAG CTGCTGCCGC CGCTGCAGCC 
GCAGCAAACC CACTTTCTGC GTTGGTCGCT GCTACAGGAG CATGTTTCTC ACTGGACACC 
TGGGTCACAA CACCCACTGG TAAAAAGAGA ATGGATCAAA TTGACATTGG CGATTATGTT 
CTGACAGCCG ATCTAGAAAA GACCTATTTC ACACCCATTA CTCTTTGGAT TCACCGCGAG 
CCGGAAAAAG TCCAAGAATT CCTTACAATT ATGACGGAAT ATGGGAAAAC TCTCCGAATC 
ACCTCGCGTC ACTTTATGTA TCGGAACAAA TGTGGAAAAT CATATCCGCA ATATATCAAA 
ATGCTCCCTC ATGACGGAGA AGCAATATTC GCATCAGACC TTGAAGTTGG AGACTGTGTT 
GTTGTACTGT ACAAGGGAAA ATATCGCCAA CAGAAAATTG AAACTATCAC CAGATCTGTG 
AGAACGGGAA TTTATTCACC GCTGACAAAC AATGGGAGAA TAATTGTAAA CGATATGCTA 
GCCTCTTGTT ATAGTGAGAT ACAGCAAAAC ACACTGCAAA CAACTTTCTT CTGGGCATAT 
GACAAACTTC GTAGTGTTCT CGTTGAATTC TTTGGAGATC TGTACAACAA CAAGATCGAA 
CTCCCAACCG GAACTACACT CTCGAGAGAT ATCATCAGTC TGATTGTTCC AATTCAAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024844.1
CDS	1..3483
Translation	MNLLIFLLCYFLGSPHYSEANKIKLKSSGRSADINHDSPCIWNKTQSWVHPYQSITVECCDEELSKLIHKTILDAGNNAKLGNLAKFIQRRSQLFYHMSFETIVSRENFAISTHYHGTHSCRVHDNNLYYLVYETPVQYDPFNMKTEDYLASIDSADPLGSTKPANLRGDFPDVREDSTAGEDLSVQPSIGNILQYPNKFAWPDPDEEVSKKIMLAEQDAIRSINELMESVTEISVMNSGAVVNSTDSLNITEIELPNPTPIPRNFANLREKDRLPENTHCDKERKDGNRCCDGRLASTMRDAMRQMATSPDFGQGKEGIIAAELQQKVQQRFQKSYEIIVSQSDFVISTYTAGDNFCKFDNKGFYILAYVSPKQYDIDEKEDEMKLAATSNKDPLGANTTMFENEAPWHVALKLDTYGDRAGYPVGSHCTQARTGSKCCSLILFNAMKSGYDSHVATSNFDPYDIRNISKAVQWSVEEILQHSAEVIVSLDDFAYATYNNNSYTCKYRVDKYHILAYTTPNHDLDNYDEMATSIIDSEPEAQIVYPAVDRTANQIPFQNTPSPYWTTPVPIPTQVYMPMQYTQQPMQPMYNQQPFFNQQPMFNQQPMYLQPAFNQAPLPYQYPAQPSQFNPFLASFRRFKRQIGRTPYPIHQNVYNDIGSAKPFNCPADLSGLSGMACCDGGLQFEANKVIDQAKQEPDFDKHNTRNLAKLMTRAVQKRFGTTFESVVAEADFSWGTNKFNGRTCKIDSQGYNALTYQSSSKPPPPSDFIDIPNDPTLGGPTGSSGGGGGGGNGGSGGGGGGGGGGSGGSGGGGSNSNSGGGGGNGGGGNGGGGNGNGGGAGDGNGGAGAGNGNGAGAGNGNGAGAGNGNGAGAGNGNGAGAGNGNGAGAGDASAAAAAAQAQAAAAAQAQAAAAAAAQQAAAAAAAANAQQAAAAAAAAANPLSALVAATGACFSLDTWVTTPTGKKRMDQIDIGDYVLTADLEKTYFTPITLWIHREPEKVQEFLTIMTEYGKTLRITSRHFMYRNKCGKSYPQYIKMLPHDGEAIFASDLEVGDCVVVLYKGKYRQQKIETITRSVRTGIYSPLTNNGRIIVNDMLASCYSEIQQNTLQTTFFWAYDKLRSVLVEFFGDLYNNKIELPTGTTLSRDIISLIVPIQK

Target ORF information:

RefSeq Version	NM_001029673.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans GRounDhog (hedgehog-like family) (grd-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029673.2

ORF Insert Sequence:

ATGAACTTGC TGATATTCTT ATTATGCTAT TTTCTTGGTT CACCTCATTA TTCAGAGGCA 
AATAAAATAA AACTGAAAAG TTCTGGCCGC TCTGCTGATA TAAACCACGA TTCTCCATGT 
ATTTGGAACA AAACTCAATC ATGGGTGCAT CCGTATCAAA GTATAACGGT AGAATGTTGC 
GATGAAGAAC TTTCCAAGCT TATCCATAAA ACTATTCTTG ACGCAGGCAA TAATGCAAAA 
CTGGGAAATC TTGCCAAGTT TATTCAACGG AGATCTCAAC TTTTCTATCA CATGAGTTTC 
GAAACTATCG TTTCAAGAGA AAACTTTGCT ATTTCAACTC ACTATCATGG AACTCATTCG 
TGTCGAGTTC ACGACAACAA TCTATATTAT TTGGTATACG AAACACCAGT TCAATATGAT 
CCTTTTAACA TGAAAACTGA AGATTATCTG GCCTCCATTG ACTCCGCTGA TCCATTGGGA 
AGTACCAAAC CCGCAAACTT ACGTGGAGAT TTTCCTGATG TACGTGAAGA TTCCACAGCT 
GGAGAAGATC TTTCTGTTCA ACCATCTATT GGCAATATTC TTCAATATCC CAACAAATTT 
GCTTGGCCAG ATCCAGATGA AGAAGTTTCC AAAAAAATCA TGCTTGCGGA ACAAGATGCA 
ATTCGTTCAA TAAATGAACT TATGGAATCA GTGACAGAGA TATCCGTTAT GAACTCTGGT 
GCAGTTGTTA ATTCAACTGA TTCTCTCAAT ATCACGGAGA TTGAATTGCC AAATCCCACT 
CCGATTCCGC GAAACTTTGC TAATTTGCGC GAAAAAGATC GACTACCCGA GAACACTCAT 
TGCGATAAAG AACGGAAAGA CGGAAATAGA TGCTGTGATG GAAGACTTGC TTCGACAATG 
AGGGATGCAA TGAGACAAAT GGCTACATCG CCAGATTTTG GACAGGGTAA AGAAGGCATT 
ATTGCTGCAG AACTTCAACA AAAAGTTCAA CAGCGCTTCC AAAAATCTTA CGAAATCATT 
GTATCCCAGT CAGACTTTGT TATTTCTACA TATACTGCTG GAGATAACTT CTGTAAATTT 
GATAACAAAG GCTTCTATAT TCTTGCTTAT GTATCACCCA AGCAATATGA TATTGATGAA 
AAAGAAGATG AGATGAAGCT TGCTGCAACT TCAAATAAAG ATCCGCTAGG AGCTAACACT 
ACGATGTTTG AAAACGAAGC ACCTTGGCAT GTCGCTTTAA AGTTGGACAC CTATGGAGAT 
AGAGCCGGAT ATCCTGTTGG GTCACACTGC ACTCAAGCAA GAACGGGGTC CAAATGTTGT 
AGTTTGATTC TATTCAATGC TATGAAGTCT GGATATGATT CTCATGTGGC AACATCAAAC 
TTTGATCCAT ATGATATTAG AAACATTTCG AAAGCGGTTC AATGGAGTGT TGAAGAAATA 
TTGCAGCACT CCGCTGAAGT GATTGTTTCG CTGGATGATT TCGCTTACGC TACATATAAC 
AATAATAGCT ACACCTGCAA ATATCGTGTT GATAAATACC ATATTCTTGC CTACACTACA 
CCGAATCATG ATCTTGATAA CTACGATGAA ATGGCAACAT CCATAATTGA TAGTGAACCA 
GAAGCCCAAA TTGTGTACCC GGCAGTCGAT AGAACAGCAA ACCAAATTCC GTTCCAAAAT 
ACACCTTCAC CATATTGGAC AACTCCAGTT CCCATCCCAA CTCAGGTTTA CATGCCAATG 
CAATACACCC AACAACCGAT GCAACCAATG TATAACCAAC AACCATTTTT TAATCAACAG 
CCCATGTTCA ATCAGCAGCC AATGTACTTG CAACCAGCTT TCAATCAAGC CCCGCTTCCA 
TATCAATACC CCGCACAACC ATCCCAGTTT AATCCATTTT TAGCTAGCTT TCGTAGATTC 
AAAAGACAGA TAGGAAGAAC ACCGTATCCA ATTCATCAAA ATGTGTACAA TGATATTGGA 
AGTGCAAAAC CTTTCAATTG TCCAGCAGAT CTGAGTGGGC TATCAGGAAT GGCTTGTTGT 
GATGGAGGCC TACAATTTGA GGCAAACAAG GTCATTGACC AAGCTAAACA AGAACCTGAT 
TTTGACAAGC ACAATACGAG AAATTTGGCA AAGTTGATGA CTCGAGCCGT GCAGAAGAGA 
TTTGGAACTA CGTTCGAGTC TGTAGTTGCC GAAGCTGATT TCTCTTGGGG AACAAACAAA 
TTCAATGGAA GAACATGCAA AATTGATTCA CAAGGGTATA ATGCTCTGAC ATATCAGTCA 
AGTTCTAAAC CACCACCACC CTCTGATTTT ATTGATATTC CAAACGATCC AACTCTTGGT 
GGTCCAACAG GCTCTAGTGG CGGTGGCGGA GGAGGAGGAA ATGGTGGAAG TGGTGGAGGC 
GGAGGAGGCG GCGGCGGAGG TTCAGGAGGA AGTGGCGGAG GTGGAAGTAA TAGTAATTCT 
GGTGGTGGAG GTGGAAATGG TGGAGGCGGA AATGGCGGAG GAGGTAATGG TAATGGAGGA 
GGTGCTGGAG ACGGAAATGG TGGAGCCGGT GCCGGTAATG GAAACGGTGC TGGAGCTGGA 
AACGGAAACG GAGCTGGAGC TGGAAATGGA AACGGCGCTG GCGCTGGTAA TGGAAATGGA 
GCAGGTGCAG GAAATGGAAA CGGAGCAGGA GCTGGAGATG CGTCCGCAGC GGCCGCGGCA 
GCCCAAGCAC AGGCTGCCGC AGCAGCTCAA GCCCAGGCAG CCGCAGCAGC TGCTGCTCAA 
CAAGCTGCCG CCGCAGCAGC AGCTGCTAAT GCTCAACAAG CTGCTGCCGC CGCTGCAGCC 
GCAGCAAACC CACTTTCTGC GTTGGTCGCT GCTACAGGAG CATGTTTCTC ACTGGACACC 
TGGGTCACAA CACCCACTGG TAAAAAGAGA ATGGATCAAA TTGACATTGG CGATTATGTT 
CTGACAGCCG ATCTAGAAAA GACCTATTTC ACACCCATTA CTCTTTGGAT TCACCGCGAG 
CCGGAAAAAG TCCAAGAATT CCTTACAATT ATGACGGAAT ATGGGAAAAC TCTCCGAATC 
ACCTCGCGTC ACTTTATGTA TCGGAACAAA TGTGGAAAAT CATATCCGCA ATATATCAAA 
ATGCTCCCTC ATGACGGAGA AGCAATATTC GCATCAGACC TTGAAGTTGG AGACTGTGTT 
GTTGTACTGT ACAAGGGAAA ATATCGCCAA CAGAAAATTG AAACTATCAC CAGATCTGTG 
AGAACGGGAA TTTATTCACC GCTGACAAAC AATGGGAGAA TAATTGTAAA CGATATGCTA 
GCCTCTTGTT ATAGTGAGAT ACAGCAAAAC ACACTGCAAA CAACTTTCTT CTGGGCATAT 
GACAAACTTC GTAGTGTTCT CGTTGAATTC TTTGGAGATC TGTACAACAA CAAGATCGAA 
CTCCCAACCG GAACTACACT CTCGAGAGAT ATCATCAGTC TGATTGTTCC AATTCAAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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grd-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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