KIAA0430 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	KIAA0430
Entrez Gene ID	106884788
Full Name	meiosis regulator and mRNA stability factor 1
Synonyms	KIAA0430
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014936373.1	XP_014791859.1	meiosis arrest female protein 1 isoform X1
XM_014936375.1	XP_014791861.1	meiosis arrest female protein 1 isoform X1
XM_014936374.1	XP_014791860.1	meiosis arrest female protein 1 isoform X1
XM_014936377.1	XP_014791863.1	meiosis arrest female protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following KIAA0430 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIAA0430 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa60899	XM_014936373.1 Latest version!	Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa60899	XM_014936375.1 Latest version!	Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa60899	XM_014936374.1 Latest version!	Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa60900	XM_014936377.1 Latest version!	Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIAA0430 ( XM_014936373.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014791859.1 Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa60899
Clone ID Related Accession (Same CDS sequence)	XM_014936373.1 , XM_014936375.1 , XM_014936374.1
Accession Version	XM_014936373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	meiosis arrest female protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090794.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG GGCGCAGGAC AGAGAACCTC TGCAATAGAT CGTTCGGATG GCTTCAGCAA 
CAAGAGAATG ATGCTAAACC ATGGCTCTGG AAACTTTCTA ATTGCTTTTC TGCTGCTGAG 
AAGTCTTTGC CTTGCAGTGC GGAAACGGAA GATTACATGG AGAACAAGAA AGCTGCTGCA 
GAATTGAAGG ATGCTTCATC TCCTCATAAT GCTGGCTCTA AACTGTTCCC AGCAGTACCG 
CTTCCTGATG TTCATCCTCT TCAGCAACAG CAAATACAGC TTTCACCTGG CCCGAAAGTA 
AGCTGCTGTG CTCATGGCTC TGACTCTTCT TGTACTCCAC AGATGCATTG TGGTGGTGGT 
GGTGGTAACT TGATTCACCC TGGCACAATA TTAGACTCAA AAAGCACTGG GACGATTACT 
TGTCAAGTAG GGTCAGGATT TGCTTATCAG TCTGCATCTT CACTGAAGAA CCCTCCAGCT 
AGAAGCAATT TGGCAGGCAT TGCGAGTGAG TTCCCTGGCA TGTGCGTAGA AAACAGTGTA 
TCTTCCTGTC AACATCTGCC CTGTTGTGGA AAACTCCATT TCCAGTCCTG TCATGGTAAT 
GTCCACAAAC TGCATCAGTT TCCAGCCCTT CAGAGCTGCC CATCTTCTGG CTATTTCCCT 
TGTTCTGAAT TCCCAAGTGG GGCCACAGGC CATTTGGATG AGCATATTGC ACAGTCGGAG 
CTAACATCGC GTGTGTGCGC CAACCCTTTG CACCTAAACA TGGCACCTTC AGTTTGTTTA 
AAGGGCTCTC ACTACTGCAG TGACTGCTTG AGCAAGCCAA CCAGAAACAG CCTAGTTGAT 
GCTGCTAAAA TCTGGCCAAA TATTCCTCCT CCAAGTGCAC AGACTGCACC TGTTCCTATC 
CCAATATGTA ATGGCTGTGG AACCAAAGGA ACAGGAAATG AGAAGAGTTT GCTACTGGCG 
AGCAGCCTTG GCAAATCACC GCAGAAATAT GGATCTCCGG AAGTTGCAAT AACAGGCCAA 
GTTCTGGAAA ACTTGCCCCC CATTGGAGTC TTCTGGGACA TTGAAAACTG TTCAGTTCCC 
ACTGGCCGTT CAGCTATAGC CGTTGTACAG AGGATACGTG AAAAGTTTTT TAAAGGTCAC 
AGAGAGGCAG AATTCATCTG TGTGTGTGAC ATTAGTAAAG AAAATAAAGA AGTTATTCAG 
GAGCTAAACA ACTGCCAGGT GACTGTTGCA CACATCAATG CTACAGCAAA GAATGCTGCT 
GATGACAAAC TCAGACAGAG TCTTAGGAGA TTTGCGGATA CCCATGCTGC ACCTGCCACT 
GTGGTTCTTG TGTCAACGGA TGTAAACTTT GCTTTGGAAC TCAGTGACCT GAGACATCGA 
CATGGTTTTC GGATCATTTT GGTACATAAA AACCAAGCTT CAGAAGCACT CTTACATCAT 
GCTCATGAGC TTATTTGTTT TGAAGAATTT ATTTCAGACT TGCCACCAAG GTTACCACTG 
AAAATGCCGG CATGCCATAC ACTGTTATAT GTTTATAACC TACCAACAAA TAGAGACAGC 
AAAAGTATCA GCAATAGACT CAGGCGTTTG TCGGACAACT GTGGAGGGAA GGTGCTGAGC 
ATTTCTGGAA GCAGTGCAAT TCTCCGTTTT TTAAATCAAG AAAGTGCTGA ACGCGCTCGG 
AAGCGAATGG AAAATGAAGA TGTTTTTGGT AACAGGATTG TAGTGTCTTT TACTCCCAAA 
ACCAAGGAAC TTAGTGAGAC AAAAAGCTCC AGCTTTGTGG GAACAGAAAA GGTGAAGTCT 
CCCAAAAAAA TTAACAAGAA CACAAAGCTG TGCCTCCTCA ACAAAGACTC AAGTGATCAG 
TCTTGTGGTA CCAAAGGCTC TGCTGGAAGA GGATTCCAGA TTCATGGATC TATCATCAAA 
CCCACAAATG TTAAAAGTTT ACAGGAGCTG TGCCGTCTTG AATCAAAGAC CATCAGTAGA 
AATACTGAAA ACCAGCAAGA ACTTTTAAGA GAACAAATTC CTTCTCCTCA GAGTAACTCT 
AATGCAGCAA TTCCTGTGTC TCTGGCAATG AAAAAAACTG GAATGGGAGA ATCATCCTGT 
AAAAGTAGTC AGAAAAGAGA GACTTCTGCT TCCAGGAGTG TTACCAATTC CCCTGTAGAT 
AAAAAAGATA AAGATGAAAC TGTGTTTCAG GTCAGCTATC CCTCTGCTTT CAGTAAGTTG 
ACGGCCTCAA GACAACTCAG TCCTTTACTC ATGTCTCAGA GTTGCTGGTC ATCTCGGAGT 
ATGTCTCCAA ACCTCTCAAA CAGATCATCT CCACTCACAT TTAATGTAGT AAATCATACC 
AGTGGTACAG ACTGCCCTGA TCCTTTTGCA AATGGTGCAG ACATTCACAT CAGCAATATA 
GATTACAGAT TGTCCAGAAA AGAGTTGCAG CAAAATTTGC ACGAAATTTT CTCAAGACAT 
GGCAAGGTAA AAAGTGTAGA GCTCAGTCCC CATACAGACT ACCAGTTAAA GGCTACAATT 
CAGATGGAAA ATCTGCAAGA AGCAATCAGT GCTGTTAACA GTCTTCACAG ATACAAAATT 
GGCAGTAAAA GGATCCAGGT CTCATTAGCA ACGGGAGCTG CTAATAAATC ACTCTCTCTC 
CTTAGCTCAG AAACAATGTC CATTCTGCAG GACGCACCTG CTTGTTGTCT GCCTGTGTTC 
AAATTCACAG AAATCTATGA AAAAAAATTT GGACGTAAGT TGATTCTATC GGATTTATAT 
AAATTAACGG ATACTGTGGC AATCCGTGAC CAAGGCAGCG GGCGGCTGGT GTGCCTCTTA 
TCCAGCAGCC AAGCCAGGCA GAGTCCATTG GGATCTTCAC AGTCACATGA TGGCTCCTCA 
GCAAACTGTA GTCCTATAAT ATTTGAAGAG TTGGAATATC ACGAGCCTGT TTGTAAGCAG 
CATTGCCTGA ATAAAGACTT TATTGAGCAT GAGTTTGATC CAGATTCTTA TAGAATTCCA 
TTTGTGATTT TGTCTCTGAA GACATTTGCT CCCCAAGTTC ATAGTCTTCT ACAGACACAT 
GAGGGTACTG TGCCTTTACT AAGTTTTCCT GATTGTTATA TGTCAGAGTT CAACGATCTT 
GAAATAGTGC CAGAAGGCCA GGGTGGTGTT GCTTTAGAAC ATCTAATTAC CTGTGTTCCT 
GGAGTTAACA TTGCCACTGC CCAGAACGGC ATTAAAGTTA TTAAATGGAT ACATAACAAA 
CCACCACCTC CTACTACAGA TCCTTGGCTT CTACGTTCTA AGAGCCCTGT GGGTAACCCA 
CAACTTATTC AGTTCAGTAG AGAAGTTATA GATCTACTTA AAAGCCAACC GTCCTGCGTC 
ATACCTGTCA GTAAATTCAT CCCAACGTAC CACCATCACT TCGCAAAGCA ATGCCGGGTG 
TCTGACTACG GGTATTCTAA ATTAATGGAG CTGCTGGAAG CAGTGCCTCA TGTGCTGCAA 
ATCCTTGGTA TGGGTTCCAA GCGCTTGTTG ACTCTAACAC ACAGAGCTCA AGTGAAGCGG 
TTTACTCAAG ACTTACTGAA GCTTCTCAAG TCCCAGGCCA GCAAGCAAGT TATTGTGAGG 
GAGTTCTTAC AGGCTTACCA CTGGTGTTTC TCTAAGGACT GGGATGTTAC TGAGTATGGA 
GTGTGTGAAC TGGCTGATAT TATATCAGAA ATTCCAGATA CAACTATCTG TTTGTCACAG 
CAAGACAATG AAATGGTAAT TTGTATCCCC AAAAGAGAAC GTACACAAGA AGAAATTGAA 
AGAACCAAAC AATTTTCTAA AGAGGTAGTA GACTTACTGC GCCACCAACC CCATTTTCGA 
ATGCCCTTCA ATAAATTTAT TCCTTCATAT CACCACCACT TTGGTCGTCA GTGCAAACTT 
GCTTACTATG GTTTTACAAA ACTACTTGAA CTCTTTGAAG CCATACCAGA TGTCTTACAT 
GTATTGGAAT GTGGGGAAGA GAAAATTCTC ACGCTCACAG AGGTGGAACA AGTCAAAGCA 
GTTGCTGCCC AGTTTGTTAA ACTGCTGCGG TCTCAGAAAG ACAACTGCCT TATGATGGCT 
GATGTGCTGA CAGAGTACAG CAAAACCTTT GGATACACGC TGCGCCTTCA TGACTACGAT 
GTTAGCTCAG TTCCAGCTTT AATGCAAAAA CTCTGCCACG TTGTAAAGGT TGTTGACACA 
GAGTCTGGCA AGCAAATTCA GCTGATAAAT AGAAAATCTC TGCGGACTCT GACTGCCCAA 
TTGCTAGTCT TGTTGATGTC CTGGGATGGA ACATCCTTTC TCTCTGTTGA ACAGCTTAAA 
CAGCATTATG AAACAACCCA TAGTACTTCC CTTAATCCTT GTGAATATGG ATTTATGACC 
TTAACTGAAC TCCTCAAGAG TCTGCCTTAC TTGGTTGAGG TTTTTACCAA TGGTGGAGCA 
GAAGAATATG TGAAGCTTAC AAATCTGTAT ATGTTTGCAA AGAATGTAAG GTCCTTACTT 
CACACCTACC ATTATCAGCA GATTTTTCTT CATGAGTTCC CAGTAGCATA TAGCAAATAC 
ACAGGAGAAG TGCTGCAACC TAAAGTATAT GGATGCAATA ATTTAGAAGA GCTTTTGGGA 
GCAATTCCAC AGGTGGTCTG GATTAAAGGA CATGGCCATA AAAGAATAGT GGTACTAAAG 
AATGATATGA AAACTCGTTT TAGCTCACCT AGTTTTCCCC CTGCTGATCA TGTGGATGAT 
CATGGAAATC AACTTGCTGA CAATAATGGA CATATTATGG AGACCCCAGG ATCTACTTCA 
TCAATGGAAC TAAGTCTAGG AACACCGAGC AATGTTTCTA ACCCAACTGA GCAAGAACTA 
CTTTGCCTCA CAAACACATC TCCCATTGAC CTCCTGTGTG AGCCAGTTCC TTCCTGCCTA 
CCGTCCCCAC AGCTGAGACC TGATCCGGTG GTTCTTGAGT CTGCAGACCT CATTCAGTTT 
GAGGAACGCC CTGCACCTCT CTCTGAGATA ATGATTTTAA CAGAAGAAGA AAAACAAAGA 
ATTGGCACCA CAGCTCAAGA AAAATTGACC TCTGGTTCCG TGGTATCTAA CACCACGGAG 
AATGCTTCAG TGCCTCCCTG CCAGTCCTCT GAAACCCAAA CCCACAAGGA AGCAATGGAC 
AGCCCAGCCA AAAAGCAACA CAAAAACAAG GTGAAATTGG CAGCAAACTT TTCACTTGCA 
CCTGTAACCA AGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014791859.1
CDS	149..5386
Translation	MMEGRRTENLCNRSFGWLQQQENDAKPWLWKLSNCFSAAEKSLPCSAETEDYMENKKAAAELKDASSPHNAGSKLFPAVPLPDVHPLQQQQIQLSPGPKVSCCAHGSDSSCTPQMHCGGGGGNLIHPGTILDSKSTGTITCQVGSGFAYQSASSLKNPPARSNLAGIASEFPGMCVENSVSSCQHLPCCGKLHFQSCHGNVHKLHQFPALQSCPSSGYFPCSEFPSGATGHLDEHIAQSELTSRVCANPLHLNMAPSVCLKGSHYCSDCLSKPTRNSLVDAAKIWPNIPPPSAQTAPVPIPICNGCGTKGTGNEKSLLLASSLGKSPQKYGSPEVAITGQVLENLPPIGVFWDIENCSVPTGRSAIAVVQRIREKFFKGHREAEFICVCDISKENKEVIQELNNCQVTVAHINATAKNAADDKLRQSLRRFADTHAAPATVVLVSTDVNFALELSDLRHRHGFRIILVHKNQASEALLHHAHELICFEEFISDLPPRLPLKMPACHTLLYVYNLPTNRDSKSISNRLRRLSDNCGGKVLSISGSSAILRFLNQESAERARKRMENEDVFGNRIVVSFTPKTKELSETKSSSFVGTEKVKSPKKINKNTKLCLLNKDSSDQSCGTKGSAGRGFQIHGSIIKPTNVKSLQELCRLESKTISRNTENQQELLREQIPSPQSNSNAAIPVSLAMKKTGMGESSCKSSQKRETSASRSVTNSPVDKKDKDETVFQVSYPSAFSKLTASRQLSPLLMSQSCWSSRSMSPNLSNRSSPLTFNVVNHTSGTDCPDPFANGADIHISNIDYRLSRKELQQNLHEIFSRHGKVKSVELSPHTDYQLKATIQMENLQEAISAVNSLHRYKIGSKRIQVSLATGAANKSLSLLSSETMSILQDAPACCLPVFKFTEIYEKKFGRKLILSDLYKLTDTVAIRDQGSGRLVCLLSSSQARQSPLGSSQSHDGSSANCSPIIFEELEYHEPVCKQHCLNKDFIEHEFDPDSYRIPFVILSLKTFAPQVHSLLQTHEGTVPLLSFPDCYMSEFNDLEIVPEGQGGVALEHLITCVPGVNIATAQNGIKVIKWIHNKPPPPTTDPWLLRSKSPVGNPQLIQFSREVIDLLKSQPSCVIPVSKFIPTYHHHFAKQCRVSDYGYSKLMELLEAVPHVLQILGMGSKRLLTLTHRAQVKRFTQDLLKLLKSQASKQVIVREFLQAYHWCFSKDWDVTEYGVCELADIISEIPDTTICLSQQDNEMVICIPKRERTQEEIERTKQFSKEVVDLLRHQPHFRMPFNKFIPSYHHHFGRQCKLAYYGFTKLLELFEAIPDVLHVLECGEEKILTLTEVEQVKAVAAQFVKLLRSQKDNCLMMADVLTEYSKTFGYTLRLHDYDVSSVPALMQKLCHVVKVVDTESGKQIQLINRKSLRTLTAQLLVLLMSWDGTSFLSVEQLKQHYETTHSTSLNPCEYGFMTLTELLKSLPYLVEVFTNGGAEEYVKLTNLYMFAKNVRSLLHTYHYQQIFLHEFPVAYSKYTGEVLQPKVYGCNNLEELLGAIPQVVWIKGHGHKRIVVLKNDMKTRFSSPSFPPADHVDDHGNQLADNNGHIMETPGSTSSMELSLGTPSNVSNPTEQELLCLTNTSPIDLLCEPVPSCLPSPQLRPDPVVLESADLIQFEERPAPLSEIMILTEEEKQRIGTTAQEKLTSGSVVSNTTENASVPPCQSSETQTHKEAMDSPAKKQHKNKVKLAANFSLAPVTKL

Target ORF information:

RefSeq Version	XM_014936373.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936373.1

ORF Insert Sequence:

ATGATGGAAG GGCGCAGGAC AGAGAACCTC TGCAATAGAT CGTTCGGATG GCTTCAGCAA 
CAAGAGAATG ATGCTAAACC ATGGCTCTGG AAACTTTCTA ATTGCTTTTC TGCTGCTGAG 
AAGTCTTTGC CTTGCAGTGC GGAAACGGAA GATTACATGG AGAACAAGAA AGCTGCTGCA 
GAATTGAAGG ATGCTTCATC TCCTCATAAT GCTGGCTCTA AACTGTTCCC AGCAGTACCG 
CTTCCTGATG TTCATCCTCT TCAGCAACAG CAAATACAGC TTTCACCTGG CCCGAAAGTA 
AGCTGCTGTG CTCATGGCTC TGACTCTTCT TGTACTCCAC AGATGCATTG TGGTGGTGGT 
GGTGGTAACT TGATTCACCC TGGCACAATA TTAGACTCAA AAAGCACTGG GACGATTACT 
TGTCAAGTAG GGTCAGGATT TGCTTATCAG TCTGCATCTT CACTGAAGAA CCCTCCAGCT 
AGAAGCAATT TGGCAGGCAT TGCGAGTGAG TTCCCTGGCA TGTGCGTAGA AAACAGTGTA 
TCTTCCTGTC AACATCTGCC CTGTTGTGGA AAACTCCATT TCCAGTCCTG TCATGGTAAT 
GTCCACAAAC TGCATCAGTT TCCAGCCCTT CAGAGCTGCC CATCTTCTGG CTATTTCCCT 
TGTTCTGAAT TCCCAAGTGG GGCCACAGGC CATTTGGATG AGCATATTGC ACAGTCGGAG 
CTAACATCGC GTGTGTGCGC CAACCCTTTG CACCTAAACA TGGCACCTTC AGTTTGTTTA 
AAGGGCTCTC ACTACTGCAG TGACTGCTTG AGCAAGCCAA CCAGAAACAG CCTAGTTGAT 
GCTGCTAAAA TCTGGCCAAA TATTCCTCCT CCAAGTGCAC AGACTGCACC TGTTCCTATC 
CCAATATGTA ATGGCTGTGG AACCAAAGGA ACAGGAAATG AGAAGAGTTT GCTACTGGCG 
AGCAGCCTTG GCAAATCACC GCAGAAATAT GGATCTCCGG AAGTTGCAAT AACAGGCCAA 
GTTCTGGAAA ACTTGCCCCC CATTGGAGTC TTCTGGGACA TTGAAAACTG TTCAGTTCCC 
ACTGGCCGTT CAGCTATAGC CGTTGTACAG AGGATACGTG AAAAGTTTTT TAAAGGTCAC 
AGAGAGGCAG AATTCATCTG TGTGTGTGAC ATTAGTAAAG AAAATAAAGA AGTTATTCAG 
GAGCTAAACA ACTGCCAGGT GACTGTTGCA CACATCAATG CTACAGCAAA GAATGCTGCT 
GATGACAAAC TCAGACAGAG TCTTAGGAGA TTTGCGGATA CCCATGCTGC ACCTGCCACT 
GTGGTTCTTG TGTCAACGGA TGTAAACTTT GCTTTGGAAC TCAGTGACCT GAGACATCGA 
CATGGTTTTC GGATCATTTT GGTACATAAA AACCAAGCTT CAGAAGCACT CTTACATCAT 
GCTCATGAGC TTATTTGTTT TGAAGAATTT ATTTCAGACT TGCCACCAAG GTTACCACTG 
AAAATGCCGG CATGCCATAC ACTGTTATAT GTTTATAACC TACCAACAAA TAGAGACAGC 
AAAAGTATCA GCAATAGACT CAGGCGTTTG TCGGACAACT GTGGAGGGAA GGTGCTGAGC 
ATTTCTGGAA GCAGTGCAAT TCTCCGTTTT TTAAATCAAG AAAGTGCTGA ACGCGCTCGG 
AAGCGAATGG AAAATGAAGA TGTTTTTGGT AACAGGATTG TAGTGTCTTT TACTCCCAAA 
ACCAAGGAAC TTAGTGAGAC AAAAAGCTCC AGCTTTGTGG GAACAGAAAA GGTGAAGTCT 
CCCAAAAAAA TTAACAAGAA CACAAAGCTG TGCCTCCTCA ACAAAGACTC AAGTGATCAG 
TCTTGTGGTA CCAAAGGCTC TGCTGGAAGA GGATTCCAGA TTCATGGATC TATCATCAAA 
CCCACAAATG TTAAAAGTTT ACAGGAGCTG TGCCGTCTTG AATCAAAGAC CATCAGTAGA 
AATACTGAAA ACCAGCAAGA ACTTTTAAGA GAACAAATTC CTTCTCCTCA GAGTAACTCT 
AATGCAGCAA TTCCTGTGTC TCTGGCAATG AAAAAAACTG GAATGGGAGA ATCATCCTGT 
AAAAGTAGTC AGAAAAGAGA GACTTCTGCT TCCAGGAGTG TTACCAATTC CCCTGTAGAT 
AAAAAAGATA AAGATGAAAC TGTGTTTCAG GTCAGCTATC CCTCTGCTTT CAGTAAGTTG 
ACGGCCTCAA GACAACTCAG TCCTTTACTC ATGTCTCAGA GTTGCTGGTC ATCTCGGAGT 
ATGTCTCCAA ACCTCTCAAA CAGATCATCT CCACTCACAT TTAATGTAGT AAATCATACC 
AGTGGTACAG ACTGCCCTGA TCCTTTTGCA AATGGTGCAG ACATTCACAT CAGCAATATA 
GATTACAGAT TGTCCAGAAA AGAGTTGCAG CAAAATTTGC ACGAAATTTT CTCAAGACAT 
GGCAAGGTAA AAAGTGTAGA GCTCAGTCCC CATACAGACT ACCAGTTAAA GGCTACAATT 
CAGATGGAAA ATCTGCAAGA AGCAATCAGT GCTGTTAACA GTCTTCACAG ATACAAAATT 
GGCAGTAAAA GGATCCAGGT CTCATTAGCA ACGGGAGCTG CTAATAAATC ACTCTCTCTC 
CTTAGCTCAG AAACAATGTC CATTCTGCAG GACGCACCTG CTTGTTGTCT GCCTGTGTTC 
AAATTCACAG AAATCTATGA AAAAAAATTT GGACGTAAGT TGATTCTATC GGATTTATAT 
AAATTAACGG ATACTGTGGC AATCCGTGAC CAAGGCAGCG GGCGGCTGGT GTGCCTCTTA 
TCCAGCAGCC AAGCCAGGCA GAGTCCATTG GGATCTTCAC AGTCACATGA TGGCTCCTCA 
GCAAACTGTA GTCCTATAAT ATTTGAAGAG TTGGAATATC ACGAGCCTGT TTGTAAGCAG 
CATTGCCTGA ATAAAGACTT TATTGAGCAT GAGTTTGATC CAGATTCTTA TAGAATTCCA 
TTTGTGATTT TGTCTCTGAA GACATTTGCT CCCCAAGTTC ATAGTCTTCT ACAGACACAT 
GAGGGTACTG TGCCTTTACT AAGTTTTCCT GATTGTTATA TGTCAGAGTT CAACGATCTT 
GAAATAGTGC CAGAAGGCCA GGGTGGTGTT GCTTTAGAAC ATCTAATTAC CTGTGTTCCT 
GGAGTTAACA TTGCCACTGC CCAGAACGGC ATTAAAGTTA TTAAATGGAT ACATAACAAA 
CCACCACCTC CTACTACAGA TCCTTGGCTT CTACGTTCTA AGAGCCCTGT GGGTAACCCA 
CAACTTATTC AGTTCAGTAG AGAAGTTATA GATCTACTTA AAAGCCAACC GTCCTGCGTC 
ATACCTGTCA GTAAATTCAT CCCAACGTAC CACCATCACT TCGCAAAGCA ATGCCGGGTG 
TCTGACTACG GGTATTCTAA ATTAATGGAG CTGCTGGAAG CAGTGCCTCA TGTGCTGCAA 
ATCCTTGGTA TGGGTTCCAA GCGCTTGTTG ACTCTAACAC ACAGAGCTCA AGTGAAGCGG 
TTTACTCAAG ACTTACTGAA GCTTCTCAAG TCCCAGGCCA GCAAGCAAGT TATTGTGAGG 
GAGTTCTTAC AGGCTTACCA CTGGTGTTTC TCTAAGGACT GGGATGTTAC TGAGTATGGA 
GTGTGTGAAC TGGCTGATAT TATATCAGAA ATTCCAGATA CAACTATCTG TTTGTCACAG 
CAAGACAATG AAATGGTAAT TTGTATCCCC AAAAGAGAAC GTACACAAGA AGAAATTGAA 
AGAACCAAAC AATTTTCTAA AGAGGTAGTA GACTTACTGC GCCACCAACC CCATTTTCGA 
ATGCCCTTCA ATAAATTTAT TCCTTCATAT CACCACCACT TTGGTCGTCA GTGCAAACTT 
GCTTACTATG GTTTTACAAA ACTACTTGAA CTCTTTGAAG CCATACCAGA TGTCTTACAT 
GTATTGGAAT GTGGGGAAGA GAAAATTCTC ACGCTCACAG AGGTGGAACA AGTCAAAGCA 
GTTGCTGCCC AGTTTGTTAA ACTGCTGCGG TCTCAGAAAG ACAACTGCCT TATGATGGCT 
GATGTGCTGA CAGAGTACAG CAAAACCTTT GGATACACGC TGCGCCTTCA TGACTACGAT 
GTTAGCTCAG TTCCAGCTTT AATGCAAAAA CTCTGCCACG TTGTAAAGGT TGTTGACACA 
GAGTCTGGCA AGCAAATTCA GCTGATAAAT AGAAAATCTC TGCGGACTCT GACTGCCCAA 
TTGCTAGTCT TGTTGATGTC CTGGGATGGA ACATCCTTTC TCTCTGTTGA ACAGCTTAAA 
CAGCATTATG AAACAACCCA TAGTACTTCC CTTAATCCTT GTGAATATGG ATTTATGACC 
TTAACTGAAC TCCTCAAGAG TCTGCCTTAC TTGGTTGAGG TTTTTACCAA TGGTGGAGCA 
GAAGAATATG TGAAGCTTAC AAATCTGTAT ATGTTTGCAA AGAATGTAAG GTCCTTACTT 
CACACCTACC ATTATCAGCA GATTTTTCTT CATGAGTTCC CAGTAGCATA TAGCAAATAC 
ACAGGAGAAG TGCTGCAACC TAAAGTATAT GGATGCAATA ATTTAGAAGA GCTTTTGGGA 
GCAATTCCAC AGGTGGTCTG GATTAAAGGA CATGGCCATA AAAGAATAGT GGTACTAAAG 
AATGATATGA AAACTCGTTT TAGCTCACCT AGTTTTCCCC CTGCTGATCA TGTGGATGAT 
CATGGAAATC AACTTGCTGA CAATAATGGA CATATTATGG AGACCCCAGG ATCTACTTCA 
TCAATGGAAC TAAGTCTAGG AACACCGAGC AATGTTTCTA ACCCAACTGA GCAAGAACTA 
CTTTGCCTCA CAAACACATC TCCCATTGAC CTCCTGTGTG AGCCAGTTCC TTCCTGCCTA 
CCGTCCCCAC AGCTGAGACC TGATCCGGTG GTTCTTGAGT CTGCAGACCT CATTCAGTTT 
GAGGAACGCC CTGCACCTCT CTCTGAGATA ATGATTTTAA CAGAAGAAGA AAAACAAAGA 
ATTGGCACCA CAGCTCAAGA AAAATTGACC TCTGGTTCCG TGGTATCTAA CACCACGGAG 
AATGCTTCAG TGCCTCCCTG CCAGTCCTCT GAAACCCAAA CCCACAAGGA AGCAATGGAC 
AGCCCAGCCA AAAAGCAACA CAAAAACAAG GTGAAATTGG CAGCAAACTT TTCACTTGCA 
CCTGTAACCA AGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA0430 ( XM_014936375.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014791861.1 Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCa60899
Clone ID Related Accession (Same CDS sequence)	XM_014936373.1 , XM_014936375.1 , XM_014936374.1
Accession Version	XM_014936375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	meiosis arrest female protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090794.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG GGCGCAGGAC AGAGAACCTC TGCAATAGAT CGTTCGGATG GCTTCAGCAA 
CAAGAGAATG ATGCTAAACC ATGGCTCTGG AAACTTTCTA ATTGCTTTTC TGCTGCTGAG 
AAGTCTTTGC CTTGCAGTGC GGAAACGGAA GATTACATGG AGAACAAGAA AGCTGCTGCA 
GAATTGAAGG ATGCTTCATC TCCTCATAAT GCTGGCTCTA AACTGTTCCC AGCAGTACCG 
CTTCCTGATG TTCATCCTCT TCAGCAACAG CAAATACAGC TTTCACCTGG CCCGAAAGTA 
AGCTGCTGTG CTCATGGCTC TGACTCTTCT TGTACTCCAC AGATGCATTG TGGTGGTGGT 
GGTGGTAACT TGATTCACCC TGGCACAATA TTAGACTCAA AAAGCACTGG GACGATTACT 
TGTCAAGTAG GGTCAGGATT TGCTTATCAG TCTGCATCTT CACTGAAGAA CCCTCCAGCT 
AGAAGCAATT TGGCAGGCAT TGCGAGTGAG TTCCCTGGCA TGTGCGTAGA AAACAGTGTA 
TCTTCCTGTC AACATCTGCC CTGTTGTGGA AAACTCCATT TCCAGTCCTG TCATGGTAAT 
GTCCACAAAC TGCATCAGTT TCCAGCCCTT CAGAGCTGCC CATCTTCTGG CTATTTCCCT 
TGTTCTGAAT TCCCAAGTGG GGCCACAGGC CATTTGGATG AGCATATTGC ACAGTCGGAG 
CTAACATCGC GTGTGTGCGC CAACCCTTTG CACCTAAACA TGGCACCTTC AGTTTGTTTA 
AAGGGCTCTC ACTACTGCAG TGACTGCTTG AGCAAGCCAA CCAGAAACAG CCTAGTTGAT 
GCTGCTAAAA TCTGGCCAAA TATTCCTCCT CCAAGTGCAC AGACTGCACC TGTTCCTATC 
CCAATATGTA ATGGCTGTGG AACCAAAGGA ACAGGAAATG AGAAGAGTTT GCTACTGGCG 
AGCAGCCTTG GCAAATCACC GCAGAAATAT GGATCTCCGG AAGTTGCAAT AACAGGCCAA 
GTTCTGGAAA ACTTGCCCCC CATTGGAGTC TTCTGGGACA TTGAAAACTG TTCAGTTCCC 
ACTGGCCGTT CAGCTATAGC CGTTGTACAG AGGATACGTG AAAAGTTTTT TAAAGGTCAC 
AGAGAGGCAG AATTCATCTG TGTGTGTGAC ATTAGTAAAG AAAATAAAGA AGTTATTCAG 
GAGCTAAACA ACTGCCAGGT GACTGTTGCA CACATCAATG CTACAGCAAA GAATGCTGCT 
GATGACAAAC TCAGACAGAG TCTTAGGAGA TTTGCGGATA CCCATGCTGC ACCTGCCACT 
GTGGTTCTTG TGTCAACGGA TGTAAACTTT GCTTTGGAAC TCAGTGACCT GAGACATCGA 
CATGGTTTTC GGATCATTTT GGTACATAAA AACCAAGCTT CAGAAGCACT CTTACATCAT 
GCTCATGAGC TTATTTGTTT TGAAGAATTT ATTTCAGACT TGCCACCAAG GTTACCACTG 
AAAATGCCGG CATGCCATAC ACTGTTATAT GTTTATAACC TACCAACAAA TAGAGACAGC 
AAAAGTATCA GCAATAGACT CAGGCGTTTG TCGGACAACT GTGGAGGGAA GGTGCTGAGC 
ATTTCTGGAA GCAGTGCAAT TCTCCGTTTT TTAAATCAAG AAAGTGCTGA ACGCGCTCGG 
AAGCGAATGG AAAATGAAGA TGTTTTTGGT AACAGGATTG TAGTGTCTTT TACTCCCAAA 
ACCAAGGAAC TTAGTGAGAC AAAAAGCTCC AGCTTTGTGG GAACAGAAAA GGTGAAGTCT 
CCCAAAAAAA TTAACAAGAA CACAAAGCTG TGCCTCCTCA ACAAAGACTC AAGTGATCAG 
TCTTGTGGTA CCAAAGGCTC TGCTGGAAGA GGATTCCAGA TTCATGGATC TATCATCAAA 
CCCACAAATG TTAAAAGTTT ACAGGAGCTG TGCCGTCTTG AATCAAAGAC CATCAGTAGA 
AATACTGAAA ACCAGCAAGA ACTTTTAAGA GAACAAATTC CTTCTCCTCA GAGTAACTCT 
AATGCAGCAA TTCCTGTGTC TCTGGCAATG AAAAAAACTG GAATGGGAGA ATCATCCTGT 
AAAAGTAGTC AGAAAAGAGA GACTTCTGCT TCCAGGAGTG TTACCAATTC CCCTGTAGAT 
AAAAAAGATA AAGATGAAAC TGTGTTTCAG GTCAGCTATC CCTCTGCTTT CAGTAAGTTG 
ACGGCCTCAA GACAACTCAG TCCTTTACTC ATGTCTCAGA GTTGCTGGTC ATCTCGGAGT 
ATGTCTCCAA ACCTCTCAAA CAGATCATCT CCACTCACAT TTAATGTAGT AAATCATACC 
AGTGGTACAG ACTGCCCTGA TCCTTTTGCA AATGGTGCAG ACATTCACAT CAGCAATATA 
GATTACAGAT TGTCCAGAAA AGAGTTGCAG CAAAATTTGC ACGAAATTTT CTCAAGACAT 
GGCAAGGTAA AAAGTGTAGA GCTCAGTCCC CATACAGACT ACCAGTTAAA GGCTACAATT 
CAGATGGAAA ATCTGCAAGA AGCAATCAGT GCTGTTAACA GTCTTCACAG ATACAAAATT 
GGCAGTAAAA GGATCCAGGT CTCATTAGCA ACGGGAGCTG CTAATAAATC ACTCTCTCTC 
CTTAGCTCAG AAACAATGTC CATTCTGCAG GACGCACCTG CTTGTTGTCT GCCTGTGTTC 
AAATTCACAG AAATCTATGA AAAAAAATTT GGACGTAAGT TGATTCTATC GGATTTATAT 
AAATTAACGG ATACTGTGGC AATCCGTGAC CAAGGCAGCG GGCGGCTGGT GTGCCTCTTA 
TCCAGCAGCC AAGCCAGGCA GAGTCCATTG GGATCTTCAC AGTCACATGA TGGCTCCTCA 
GCAAACTGTA GTCCTATAAT ATTTGAAGAG TTGGAATATC ACGAGCCTGT TTGTAAGCAG 
CATTGCCTGA ATAAAGACTT TATTGAGCAT GAGTTTGATC CAGATTCTTA TAGAATTCCA 
TTTGTGATTT TGTCTCTGAA GACATTTGCT CCCCAAGTTC ATAGTCTTCT ACAGACACAT 
GAGGGTACTG TGCCTTTACT AAGTTTTCCT GATTGTTATA TGTCAGAGTT CAACGATCTT 
GAAATAGTGC CAGAAGGCCA GGGTGGTGTT GCTTTAGAAC ATCTAATTAC CTGTGTTCCT 
GGAGTTAACA TTGCCACTGC CCAGAACGGC ATTAAAGTTA TTAAATGGAT ACATAACAAA 
CCACCACCTC CTACTACAGA TCCTTGGCTT CTACGTTCTA AGAGCCCTGT GGGTAACCCA 
CAACTTATTC AGTTCAGTAG AGAAGTTATA GATCTACTTA AAAGCCAACC GTCCTGCGTC 
ATACCTGTCA GTAAATTCAT CCCAACGTAC CACCATCACT TCGCAAAGCA ATGCCGGGTG 
TCTGACTACG GGTATTCTAA ATTAATGGAG CTGCTGGAAG CAGTGCCTCA TGTGCTGCAA 
ATCCTTGGTA TGGGTTCCAA GCGCTTGTTG ACTCTAACAC ACAGAGCTCA AGTGAAGCGG 
TTTACTCAAG ACTTACTGAA GCTTCTCAAG TCCCAGGCCA GCAAGCAAGT TATTGTGAGG 
GAGTTCTTAC AGGCTTACCA CTGGTGTTTC TCTAAGGACT GGGATGTTAC TGAGTATGGA 
GTGTGTGAAC TGGCTGATAT TATATCAGAA ATTCCAGATA CAACTATCTG TTTGTCACAG 
CAAGACAATG AAATGGTAAT TTGTATCCCC AAAAGAGAAC GTACACAAGA AGAAATTGAA 
AGAACCAAAC AATTTTCTAA AGAGGTAGTA GACTTACTGC GCCACCAACC CCATTTTCGA 
ATGCCCTTCA ATAAATTTAT TCCTTCATAT CACCACCACT TTGGTCGTCA GTGCAAACTT 
GCTTACTATG GTTTTACAAA ACTACTTGAA CTCTTTGAAG CCATACCAGA TGTCTTACAT 
GTATTGGAAT GTGGGGAAGA GAAAATTCTC ACGCTCACAG AGGTGGAACA AGTCAAAGCA 
GTTGCTGCCC AGTTTGTTAA ACTGCTGCGG TCTCAGAAAG ACAACTGCCT TATGATGGCT 
GATGTGCTGA CAGAGTACAG CAAAACCTTT GGATACACGC TGCGCCTTCA TGACTACGAT 
GTTAGCTCAG TTCCAGCTTT AATGCAAAAA CTCTGCCACG TTGTAAAGGT TGTTGACACA 
GAGTCTGGCA AGCAAATTCA GCTGATAAAT AGAAAATCTC TGCGGACTCT GACTGCCCAA 
TTGCTAGTCT TGTTGATGTC CTGGGATGGA ACATCCTTTC TCTCTGTTGA ACAGCTTAAA 
CAGCATTATG AAACAACCCA TAGTACTTCC CTTAATCCTT GTGAATATGG ATTTATGACC 
TTAACTGAAC TCCTCAAGAG TCTGCCTTAC TTGGTTGAGG TTTTTACCAA TGGTGGAGCA 
GAAGAATATG TGAAGCTTAC AAATCTGTAT ATGTTTGCAA AGAATGTAAG GTCCTTACTT 
CACACCTACC ATTATCAGCA GATTTTTCTT CATGAGTTCC CAGTAGCATA TAGCAAATAC 
ACAGGAGAAG TGCTGCAACC TAAAGTATAT GGATGCAATA ATTTAGAAGA GCTTTTGGGA 
GCAATTCCAC AGGTGGTCTG GATTAAAGGA CATGGCCATA AAAGAATAGT GGTACTAAAG 
AATGATATGA AAACTCGTTT TAGCTCACCT AGTTTTCCCC CTGCTGATCA TGTGGATGAT 
CATGGAAATC AACTTGCTGA CAATAATGGA CATATTATGG AGACCCCAGG ATCTACTTCA 
TCAATGGAAC TAAGTCTAGG AACACCGAGC AATGTTTCTA ACCCAACTGA GCAAGAACTA 
CTTTGCCTCA CAAACACATC TCCCATTGAC CTCCTGTGTG AGCCAGTTCC TTCCTGCCTA 
CCGTCCCCAC AGCTGAGACC TGATCCGGTG GTTCTTGAGT CTGCAGACCT CATTCAGTTT 
GAGGAACGCC CTGCACCTCT CTCTGAGATA ATGATTTTAA CAGAAGAAGA AAAACAAAGA 
ATTGGCACCA CAGCTCAAGA AAAATTGACC TCTGGTTCCG TGGTATCTAA CACCACGGAG 
AATGCTTCAG TGCCTCCCTG CCAGTCCTCT GAAACCCAAA CCCACAAGGA AGCAATGGAC 
AGCCCAGCCA AAAAGCAACA CAAAAACAAG GTGAAATTGG CAGCAAACTT TTCACTTGCA 
CCTGTAACCA AGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014791861.1
CDS	1..5238
Translation	MMEGRRTENLCNRSFGWLQQQENDAKPWLWKLSNCFSAAEKSLPCSAETEDYMENKKAAAELKDASSPHNAGSKLFPAVPLPDVHPLQQQQIQLSPGPKVSCCAHGSDSSCTPQMHCGGGGGNLIHPGTILDSKSTGTITCQVGSGFAYQSASSLKNPPARSNLAGIASEFPGMCVENSVSSCQHLPCCGKLHFQSCHGNVHKLHQFPALQSCPSSGYFPCSEFPSGATGHLDEHIAQSELTSRVCANPLHLNMAPSVCLKGSHYCSDCLSKPTRNSLVDAAKIWPNIPPPSAQTAPVPIPICNGCGTKGTGNEKSLLLASSLGKSPQKYGSPEVAITGQVLENLPPIGVFWDIENCSVPTGRSAIAVVQRIREKFFKGHREAEFICVCDISKENKEVIQELNNCQVTVAHINATAKNAADDKLRQSLRRFADTHAAPATVVLVSTDVNFALELSDLRHRHGFRIILVHKNQASEALLHHAHELICFEEFISDLPPRLPLKMPACHTLLYVYNLPTNRDSKSISNRLRRLSDNCGGKVLSISGSSAILRFLNQESAERARKRMENEDVFGNRIVVSFTPKTKELSETKSSSFVGTEKVKSPKKINKNTKLCLLNKDSSDQSCGTKGSAGRGFQIHGSIIKPTNVKSLQELCRLESKTISRNTENQQELLREQIPSPQSNSNAAIPVSLAMKKTGMGESSCKSSQKRETSASRSVTNSPVDKKDKDETVFQVSYPSAFSKLTASRQLSPLLMSQSCWSSRSMSPNLSNRSSPLTFNVVNHTSGTDCPDPFANGADIHISNIDYRLSRKELQQNLHEIFSRHGKVKSVELSPHTDYQLKATIQMENLQEAISAVNSLHRYKIGSKRIQVSLATGAANKSLSLLSSETMSILQDAPACCLPVFKFTEIYEKKFGRKLILSDLYKLTDTVAIRDQGSGRLVCLLSSSQARQSPLGSSQSHDGSSANCSPIIFEELEYHEPVCKQHCLNKDFIEHEFDPDSYRIPFVILSLKTFAPQVHSLLQTHEGTVPLLSFPDCYMSEFNDLEIVPEGQGGVALEHLITCVPGVNIATAQNGIKVIKWIHNKPPPPTTDPWLLRSKSPVGNPQLIQFSREVIDLLKSQPSCVIPVSKFIPTYHHHFAKQCRVSDYGYSKLMELLEAVPHVLQILGMGSKRLLTLTHRAQVKRFTQDLLKLLKSQASKQVIVREFLQAYHWCFSKDWDVTEYGVCELADIISEIPDTTICLSQQDNEMVICIPKRERTQEEIERTKQFSKEVVDLLRHQPHFRMPFNKFIPSYHHHFGRQCKLAYYGFTKLLELFEAIPDVLHVLECGEEKILTLTEVEQVKAVAAQFVKLLRSQKDNCLMMADVLTEYSKTFGYTLRLHDYDVSSVPALMQKLCHVVKVVDTESGKQIQLINRKSLRTLTAQLLVLLMSWDGTSFLSVEQLKQHYETTHSTSLNPCEYGFMTLTELLKSLPYLVEVFTNGGAEEYVKLTNLYMFAKNVRSLLHTYHYQQIFLHEFPVAYSKYTGEVLQPKVYGCNNLEELLGAIPQVVWIKGHGHKRIVVLKNDMKTRFSSPSFPPADHVDDHGNQLADNNGHIMETPGSTSSMELSLGTPSNVSNPTEQELLCLTNTSPIDLLCEPVPSCLPSPQLRPDPVVLESADLIQFEERPAPLSEIMILTEEEKQRIGTTAQEKLTSGSVVSNTTENASVPPCQSSETQTHKEAMDSPAKKQHKNKVKLAANFSLAPVTKL

Target ORF information:

RefSeq Version	XM_014936375.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936375.1

ORF Insert Sequence:

ATGATGGAAG GGCGCAGGAC AGAGAACCTC TGCAATAGAT CGTTCGGATG GCTTCAGCAA 
CAAGAGAATG ATGCTAAACC ATGGCTCTGG AAACTTTCTA ATTGCTTTTC TGCTGCTGAG 
AAGTCTTTGC CTTGCAGTGC GGAAACGGAA GATTACATGG AGAACAAGAA AGCTGCTGCA 
GAATTGAAGG ATGCTTCATC TCCTCATAAT GCTGGCTCTA AACTGTTCCC AGCAGTACCG 
CTTCCTGATG TTCATCCTCT TCAGCAACAG CAAATACAGC TTTCACCTGG CCCGAAAGTA 
AGCTGCTGTG CTCATGGCTC TGACTCTTCT TGTACTCCAC AGATGCATTG TGGTGGTGGT 
GGTGGTAACT TGATTCACCC TGGCACAATA TTAGACTCAA AAAGCACTGG GACGATTACT 
TGTCAAGTAG GGTCAGGATT TGCTTATCAG TCTGCATCTT CACTGAAGAA CCCTCCAGCT 
AGAAGCAATT TGGCAGGCAT TGCGAGTGAG TTCCCTGGCA TGTGCGTAGA AAACAGTGTA 
TCTTCCTGTC AACATCTGCC CTGTTGTGGA AAACTCCATT TCCAGTCCTG TCATGGTAAT 
GTCCACAAAC TGCATCAGTT TCCAGCCCTT CAGAGCTGCC CATCTTCTGG CTATTTCCCT 
TGTTCTGAAT TCCCAAGTGG GGCCACAGGC CATTTGGATG AGCATATTGC ACAGTCGGAG 
CTAACATCGC GTGTGTGCGC CAACCCTTTG CACCTAAACA TGGCACCTTC AGTTTGTTTA 
AAGGGCTCTC ACTACTGCAG TGACTGCTTG AGCAAGCCAA CCAGAAACAG CCTAGTTGAT 
GCTGCTAAAA TCTGGCCAAA TATTCCTCCT CCAAGTGCAC AGACTGCACC TGTTCCTATC 
CCAATATGTA ATGGCTGTGG AACCAAAGGA ACAGGAAATG AGAAGAGTTT GCTACTGGCG 
AGCAGCCTTG GCAAATCACC GCAGAAATAT GGATCTCCGG AAGTTGCAAT AACAGGCCAA 
GTTCTGGAAA ACTTGCCCCC CATTGGAGTC TTCTGGGACA TTGAAAACTG TTCAGTTCCC 
ACTGGCCGTT CAGCTATAGC CGTTGTACAG AGGATACGTG AAAAGTTTTT TAAAGGTCAC 
AGAGAGGCAG AATTCATCTG TGTGTGTGAC ATTAGTAAAG AAAATAAAGA AGTTATTCAG 
GAGCTAAACA ACTGCCAGGT GACTGTTGCA CACATCAATG CTACAGCAAA GAATGCTGCT 
GATGACAAAC TCAGACAGAG TCTTAGGAGA TTTGCGGATA CCCATGCTGC ACCTGCCACT 
GTGGTTCTTG TGTCAACGGA TGTAAACTTT GCTTTGGAAC TCAGTGACCT GAGACATCGA 
CATGGTTTTC GGATCATTTT GGTACATAAA AACCAAGCTT CAGAAGCACT CTTACATCAT 
GCTCATGAGC TTATTTGTTT TGAAGAATTT ATTTCAGACT TGCCACCAAG GTTACCACTG 
AAAATGCCGG CATGCCATAC ACTGTTATAT GTTTATAACC TACCAACAAA TAGAGACAGC 
AAAAGTATCA GCAATAGACT CAGGCGTTTG TCGGACAACT GTGGAGGGAA GGTGCTGAGC 
ATTTCTGGAA GCAGTGCAAT TCTCCGTTTT TTAAATCAAG AAAGTGCTGA ACGCGCTCGG 
AAGCGAATGG AAAATGAAGA TGTTTTTGGT AACAGGATTG TAGTGTCTTT TACTCCCAAA 
ACCAAGGAAC TTAGTGAGAC AAAAAGCTCC AGCTTTGTGG GAACAGAAAA GGTGAAGTCT 
CCCAAAAAAA TTAACAAGAA CACAAAGCTG TGCCTCCTCA ACAAAGACTC AAGTGATCAG 
TCTTGTGGTA CCAAAGGCTC TGCTGGAAGA GGATTCCAGA TTCATGGATC TATCATCAAA 
CCCACAAATG TTAAAAGTTT ACAGGAGCTG TGCCGTCTTG AATCAAAGAC CATCAGTAGA 
AATACTGAAA ACCAGCAAGA ACTTTTAAGA GAACAAATTC CTTCTCCTCA GAGTAACTCT 
AATGCAGCAA TTCCTGTGTC TCTGGCAATG AAAAAAACTG GAATGGGAGA ATCATCCTGT 
AAAAGTAGTC AGAAAAGAGA GACTTCTGCT TCCAGGAGTG TTACCAATTC CCCTGTAGAT 
AAAAAAGATA AAGATGAAAC TGTGTTTCAG GTCAGCTATC CCTCTGCTTT CAGTAAGTTG 
ACGGCCTCAA GACAACTCAG TCCTTTACTC ATGTCTCAGA GTTGCTGGTC ATCTCGGAGT 
ATGTCTCCAA ACCTCTCAAA CAGATCATCT CCACTCACAT TTAATGTAGT AAATCATACC 
AGTGGTACAG ACTGCCCTGA TCCTTTTGCA AATGGTGCAG ACATTCACAT CAGCAATATA 
GATTACAGAT TGTCCAGAAA AGAGTTGCAG CAAAATTTGC ACGAAATTTT CTCAAGACAT 
GGCAAGGTAA AAAGTGTAGA GCTCAGTCCC CATACAGACT ACCAGTTAAA GGCTACAATT 
CAGATGGAAA ATCTGCAAGA AGCAATCAGT GCTGTTAACA GTCTTCACAG ATACAAAATT 
GGCAGTAAAA GGATCCAGGT CTCATTAGCA ACGGGAGCTG CTAATAAATC ACTCTCTCTC 
CTTAGCTCAG AAACAATGTC CATTCTGCAG GACGCACCTG CTTGTTGTCT GCCTGTGTTC 
AAATTCACAG AAATCTATGA AAAAAAATTT GGACGTAAGT TGATTCTATC GGATTTATAT 
AAATTAACGG ATACTGTGGC AATCCGTGAC CAAGGCAGCG GGCGGCTGGT GTGCCTCTTA 
TCCAGCAGCC AAGCCAGGCA GAGTCCATTG GGATCTTCAC AGTCACATGA TGGCTCCTCA 
GCAAACTGTA GTCCTATAAT ATTTGAAGAG TTGGAATATC ACGAGCCTGT TTGTAAGCAG 
CATTGCCTGA ATAAAGACTT TATTGAGCAT GAGTTTGATC CAGATTCTTA TAGAATTCCA 
TTTGTGATTT TGTCTCTGAA GACATTTGCT CCCCAAGTTC ATAGTCTTCT ACAGACACAT 
GAGGGTACTG TGCCTTTACT AAGTTTTCCT GATTGTTATA TGTCAGAGTT CAACGATCTT 
GAAATAGTGC CAGAAGGCCA GGGTGGTGTT GCTTTAGAAC ATCTAATTAC CTGTGTTCCT 
GGAGTTAACA TTGCCACTGC CCAGAACGGC ATTAAAGTTA TTAAATGGAT ACATAACAAA 
CCACCACCTC CTACTACAGA TCCTTGGCTT CTACGTTCTA AGAGCCCTGT GGGTAACCCA 
CAACTTATTC AGTTCAGTAG AGAAGTTATA GATCTACTTA AAAGCCAACC GTCCTGCGTC 
ATACCTGTCA GTAAATTCAT CCCAACGTAC CACCATCACT TCGCAAAGCA ATGCCGGGTG 
TCTGACTACG GGTATTCTAA ATTAATGGAG CTGCTGGAAG CAGTGCCTCA TGTGCTGCAA 
ATCCTTGGTA TGGGTTCCAA GCGCTTGTTG ACTCTAACAC ACAGAGCTCA AGTGAAGCGG 
TTTACTCAAG ACTTACTGAA GCTTCTCAAG TCCCAGGCCA GCAAGCAAGT TATTGTGAGG 
GAGTTCTTAC AGGCTTACCA CTGGTGTTTC TCTAAGGACT GGGATGTTAC TGAGTATGGA 
GTGTGTGAAC TGGCTGATAT TATATCAGAA ATTCCAGATA CAACTATCTG TTTGTCACAG 
CAAGACAATG AAATGGTAAT TTGTATCCCC AAAAGAGAAC GTACACAAGA AGAAATTGAA 
AGAACCAAAC AATTTTCTAA AGAGGTAGTA GACTTACTGC GCCACCAACC CCATTTTCGA 
ATGCCCTTCA ATAAATTTAT TCCTTCATAT CACCACCACT TTGGTCGTCA GTGCAAACTT 
GCTTACTATG GTTTTACAAA ACTACTTGAA CTCTTTGAAG CCATACCAGA TGTCTTACAT 
GTATTGGAAT GTGGGGAAGA GAAAATTCTC ACGCTCACAG AGGTGGAACA AGTCAAAGCA 
GTTGCTGCCC AGTTTGTTAA ACTGCTGCGG TCTCAGAAAG ACAACTGCCT TATGATGGCT 
GATGTGCTGA CAGAGTACAG CAAAACCTTT GGATACACGC TGCGCCTTCA TGACTACGAT 
GTTAGCTCAG TTCCAGCTTT AATGCAAAAA CTCTGCCACG TTGTAAAGGT TGTTGACACA 
GAGTCTGGCA AGCAAATTCA GCTGATAAAT AGAAAATCTC TGCGGACTCT GACTGCCCAA 
TTGCTAGTCT TGTTGATGTC CTGGGATGGA ACATCCTTTC TCTCTGTTGA ACAGCTTAAA 
CAGCATTATG AAACAACCCA TAGTACTTCC CTTAATCCTT GTGAATATGG ATTTATGACC 
TTAACTGAAC TCCTCAAGAG TCTGCCTTAC TTGGTTGAGG TTTTTACCAA TGGTGGAGCA 
GAAGAATATG TGAAGCTTAC AAATCTGTAT ATGTTTGCAA AGAATGTAAG GTCCTTACTT 
CACACCTACC ATTATCAGCA GATTTTTCTT CATGAGTTCC CAGTAGCATA TAGCAAATAC 
ACAGGAGAAG TGCTGCAACC TAAAGTATAT GGATGCAATA ATTTAGAAGA GCTTTTGGGA 
GCAATTCCAC AGGTGGTCTG GATTAAAGGA CATGGCCATA AAAGAATAGT GGTACTAAAG 
AATGATATGA AAACTCGTTT TAGCTCACCT AGTTTTCCCC CTGCTGATCA TGTGGATGAT 
CATGGAAATC AACTTGCTGA CAATAATGGA CATATTATGG AGACCCCAGG ATCTACTTCA 
TCAATGGAAC TAAGTCTAGG AACACCGAGC AATGTTTCTA ACCCAACTGA GCAAGAACTA 
CTTTGCCTCA CAAACACATC TCCCATTGAC CTCCTGTGTG AGCCAGTTCC TTCCTGCCTA 
CCGTCCCCAC AGCTGAGACC TGATCCGGTG GTTCTTGAGT CTGCAGACCT CATTCAGTTT 
GAGGAACGCC CTGCACCTCT CTCTGAGATA ATGATTTTAA CAGAAGAAGA AAAACAAAGA 
ATTGGCACCA CAGCTCAAGA AAAATTGACC TCTGGTTCCG TGGTATCTAA CACCACGGAG 
AATGCTTCAG TGCCTCCCTG CCAGTCCTCT GAAACCCAAA CCCACAAGGA AGCAATGGAC 
AGCCCAGCCA AAAAGCAACA CAAAAACAAG GTGAAATTGG CAGCAAACTT TTCACTTGCA 
CCTGTAACCA AGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA0430 ( XM_014936374.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014791860.1 Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X2, mRNA.

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CloneID	OCa60899
Clone ID Related Accession (Same CDS sequence)	XM_014936373.1 , XM_014936375.1 , XM_014936374.1
Accession Version	XM_014936374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	meiosis arrest female protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090794.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG GGCGCAGGAC AGAGAACCTC TGCAATAGAT CGTTCGGATG GCTTCAGCAA 
CAAGAGAATG ATGCTAAACC ATGGCTCTGG AAACTTTCTA ATTGCTTTTC TGCTGCTGAG 
AAGTCTTTGC CTTGCAGTGC GGAAACGGAA GATTACATGG AGAACAAGAA AGCTGCTGCA 
GAATTGAAGG ATGCTTCATC TCCTCATAAT GCTGGCTCTA AACTGTTCCC AGCAGTACCG 
CTTCCTGATG TTCATCCTCT TCAGCAACAG CAAATACAGC TTTCACCTGG CCCGAAAGTA 
AGCTGCTGTG CTCATGGCTC TGACTCTTCT TGTACTCCAC AGATGCATTG TGGTGGTGGT 
GGTGGTAACT TGATTCACCC TGGCACAATA TTAGACTCAA AAAGCACTGG GACGATTACT 
TGTCAAGTAG GGTCAGGATT TGCTTATCAG TCTGCATCTT CACTGAAGAA CCCTCCAGCT 
AGAAGCAATT TGGCAGGCAT TGCGAGTGAG TTCCCTGGCA TGTGCGTAGA AAACAGTGTA 
TCTTCCTGTC AACATCTGCC CTGTTGTGGA AAACTCCATT TCCAGTCCTG TCATGGTAAT 
GTCCACAAAC TGCATCAGTT TCCAGCCCTT CAGAGCTGCC CATCTTCTGG CTATTTCCCT 
TGTTCTGAAT TCCCAAGTGG GGCCACAGGC CATTTGGATG AGCATATTGC ACAGTCGGAG 
CTAACATCGC GTGTGTGCGC CAACCCTTTG CACCTAAACA TGGCACCTTC AGTTTGTTTA 
AAGGGCTCTC ACTACTGCAG TGACTGCTTG AGCAAGCCAA CCAGAAACAG CCTAGTTGAT 
GCTGCTAAAA TCTGGCCAAA TATTCCTCCT CCAAGTGCAC AGACTGCACC TGTTCCTATC 
CCAATATGTA ATGGCTGTGG AACCAAAGGA ACAGGAAATG AGAAGAGTTT GCTACTGGCG 
AGCAGCCTTG GCAAATCACC GCAGAAATAT GGATCTCCGG AAGTTGCAAT AACAGGCCAA 
GTTCTGGAAA ACTTGCCCCC CATTGGAGTC TTCTGGGACA TTGAAAACTG TTCAGTTCCC 
ACTGGCCGTT CAGCTATAGC CGTTGTACAG AGGATACGTG AAAAGTTTTT TAAAGGTCAC 
AGAGAGGCAG AATTCATCTG TGTGTGTGAC ATTAGTAAAG AAAATAAAGA AGTTATTCAG 
GAGCTAAACA ACTGCCAGGT GACTGTTGCA CACATCAATG CTACAGCAAA GAATGCTGCT 
GATGACAAAC TCAGACAGAG TCTTAGGAGA TTTGCGGATA CCCATGCTGC ACCTGCCACT 
GTGGTTCTTG TGTCAACGGA TGTAAACTTT GCTTTGGAAC TCAGTGACCT GAGACATCGA 
CATGGTTTTC GGATCATTTT GGTACATAAA AACCAAGCTT CAGAAGCACT CTTACATCAT 
GCTCATGAGC TTATTTGTTT TGAAGAATTT ATTTCAGACT TGCCACCAAG GTTACCACTG 
AAAATGCCGG CATGCCATAC ACTGTTATAT GTTTATAACC TACCAACAAA TAGAGACAGC 
AAAAGTATCA GCAATAGACT CAGGCGTTTG TCGGACAACT GTGGAGGGAA GGTGCTGAGC 
ATTTCTGGAA GCAGTGCAAT TCTCCGTTTT TTAAATCAAG AAAGTGCTGA ACGCGCTCGG 
AAGCGAATGG AAAATGAAGA TGTTTTTGGT AACAGGATTG TAGTGTCTTT TACTCCCAAA 
ACCAAGGAAC TTAGTGAGAC AAAAAGCTCC AGCTTTGTGG GAACAGAAAA GGTGAAGTCT 
CCCAAAAAAA TTAACAAGAA CACAAAGCTG TGCCTCCTCA ACAAAGACTC AAGTGATCAG 
TCTTGTGGTA CCAAAGGCTC TGCTGGAAGA GGATTCCAGA TTCATGGATC TATCATCAAA 
CCCACAAATG TTAAAAGTTT ACAGGAGCTG TGCCGTCTTG AATCAAAGAC CATCAGTAGA 
AATACTGAAA ACCAGCAAGA ACTTTTAAGA GAACAAATTC CTTCTCCTCA GAGTAACTCT 
AATGCAGCAA TTCCTGTGTC TCTGGCAATG AAAAAAACTG GAATGGGAGA ATCATCCTGT 
AAAAGTAGTC AGAAAAGAGA GACTTCTGCT TCCAGGAGTG TTACCAATTC CCCTGTAGAT 
AAAAAAGATA AAGATGAAAC TGTGTTTCAG GTCAGCTATC CCTCTGCTTT CAGTAAGTTG 
ACGGCCTCAA GACAACTCAG TCCTTTACTC ATGTCTCAGA GTTGCTGGTC ATCTCGGAGT 
ATGTCTCCAA ACCTCTCAAA CAGATCATCT CCACTCACAT TTAATGTAGT AAATCATACC 
AGTGGTACAG ACTGCCCTGA TCCTTTTGCA AATGGTGCAG ACATTCACAT CAGCAATATA 
GATTACAGAT TGTCCAGAAA AGAGTTGCAG CAAAATTTGC ACGAAATTTT CTCAAGACAT 
GGCAAGGTAA AAAGTGTAGA GCTCAGTCCC CATACAGACT ACCAGTTAAA GGCTACAATT 
CAGATGGAAA ATCTGCAAGA AGCAATCAGT GCTGTTAACA GTCTTCACAG ATACAAAATT 
GGCAGTAAAA GGATCCAGGT CTCATTAGCA ACGGGAGCTG CTAATAAATC ACTCTCTCTC 
CTTAGCTCAG AAACAATGTC CATTCTGCAG GACGCACCTG CTTGTTGTCT GCCTGTGTTC 
AAATTCACAG AAATCTATGA AAAAAAATTT GGACGTAAGT TGATTCTATC GGATTTATAT 
AAATTAACGG ATACTGTGGC AATCCGTGAC CAAGGCAGCG GGCGGCTGGT GTGCCTCTTA 
TCCAGCAGCC AAGCCAGGCA GAGTCCATTG GGATCTTCAC AGTCACATGA TGGCTCCTCA 
GCAAACTGTA GTCCTATAAT ATTTGAAGAG TTGGAATATC ACGAGCCTGT TTGTAAGCAG 
CATTGCCTGA ATAAAGACTT TATTGAGCAT GAGTTTGATC CAGATTCTTA TAGAATTCCA 
TTTGTGATTT TGTCTCTGAA GACATTTGCT CCCCAAGTTC ATAGTCTTCT ACAGACACAT 
GAGGGTACTG TGCCTTTACT AAGTTTTCCT GATTGTTATA TGTCAGAGTT CAACGATCTT 
GAAATAGTGC CAGAAGGCCA GGGTGGTGTT GCTTTAGAAC ATCTAATTAC CTGTGTTCCT 
GGAGTTAACA TTGCCACTGC CCAGAACGGC ATTAAAGTTA TTAAATGGAT ACATAACAAA 
CCACCACCTC CTACTACAGA TCCTTGGCTT CTACGTTCTA AGAGCCCTGT GGGTAACCCA 
CAACTTATTC AGTTCAGTAG AGAAGTTATA GATCTACTTA AAAGCCAACC GTCCTGCGTC 
ATACCTGTCA GTAAATTCAT CCCAACGTAC CACCATCACT TCGCAAAGCA ATGCCGGGTG 
TCTGACTACG GGTATTCTAA ATTAATGGAG CTGCTGGAAG CAGTGCCTCA TGTGCTGCAA 
ATCCTTGGTA TGGGTTCCAA GCGCTTGTTG ACTCTAACAC ACAGAGCTCA AGTGAAGCGG 
TTTACTCAAG ACTTACTGAA GCTTCTCAAG TCCCAGGCCA GCAAGCAAGT TATTGTGAGG 
GAGTTCTTAC AGGCTTACCA CTGGTGTTTC TCTAAGGACT GGGATGTTAC TGAGTATGGA 
GTGTGTGAAC TGGCTGATAT TATATCAGAA ATTCCAGATA CAACTATCTG TTTGTCACAG 
CAAGACAATG AAATGGTAAT TTGTATCCCC AAAAGAGAAC GTACACAAGA AGAAATTGAA 
AGAACCAAAC AATTTTCTAA AGAGGTAGTA GACTTACTGC GCCACCAACC CCATTTTCGA 
ATGCCCTTCA ATAAATTTAT TCCTTCATAT CACCACCACT TTGGTCGTCA GTGCAAACTT 
GCTTACTATG GTTTTACAAA ACTACTTGAA CTCTTTGAAG CCATACCAGA TGTCTTACAT 
GTATTGGAAT GTGGGGAAGA GAAAATTCTC ACGCTCACAG AGGTGGAACA AGTCAAAGCA 
GTTGCTGCCC AGTTTGTTAA ACTGCTGCGG TCTCAGAAAG ACAACTGCCT TATGATGGCT 
GATGTGCTGA CAGAGTACAG CAAAACCTTT GGATACACGC TGCGCCTTCA TGACTACGAT 
GTTAGCTCAG TTCCAGCTTT AATGCAAAAA CTCTGCCACG TTGTAAAGGT TGTTGACACA 
GAGTCTGGCA AGCAAATTCA GCTGATAAAT AGAAAATCTC TGCGGACTCT GACTGCCCAA 
TTGCTAGTCT TGTTGATGTC CTGGGATGGA ACATCCTTTC TCTCTGTTGA ACAGCTTAAA 
CAGCATTATG AAACAACCCA TAGTACTTCC CTTAATCCTT GTGAATATGG ATTTATGACC 
TTAACTGAAC TCCTCAAGAG TCTGCCTTAC TTGGTTGAGG TTTTTACCAA TGGTGGAGCA 
GAAGAATATG TGAAGCTTAC AAATCTGTAT ATGTTTGCAA AGAATGTAAG GTCCTTACTT 
CACACCTACC ATTATCAGCA GATTTTTCTT CATGAGTTCC CAGTAGCATA TAGCAAATAC 
ACAGGAGAAG TGCTGCAACC TAAAGTATAT GGATGCAATA ATTTAGAAGA GCTTTTGGGA 
GCAATTCCAC AGGTGGTCTG GATTAAAGGA CATGGCCATA AAAGAATAGT GGTACTAAAG 
AATGATATGA AAACTCGTTT TAGCTCACCT AGTTTTCCCC CTGCTGATCA TGTGGATGAT 
CATGGAAATC AACTTGCTGA CAATAATGGA CATATTATGG AGACCCCAGG ATCTACTTCA 
TCAATGGAAC TAAGTCTAGG AACACCGAGC AATGTTTCTA ACCCAACTGA GCAAGAACTA 
CTTTGCCTCA CAAACACATC TCCCATTGAC CTCCTGTGTG AGCCAGTTCC TTCCTGCCTA 
CCGTCCCCAC AGCTGAGACC TGATCCGGTG GTTCTTGAGT CTGCAGACCT CATTCAGTTT 
GAGGAACGCC CTGCACCTCT CTCTGAGATA ATGATTTTAA CAGAAGAAGA AAAACAAAGA 
ATTGGCACCA CAGCTCAAGA AAAATTGACC TCTGGTTCCG TGGTATCTAA CACCACGGAG 
AATGCTTCAG TGCCTCCCTG CCAGTCCTCT GAAACCCAAA CCCACAAGGA AGCAATGGAC 
AGCCCAGCCA AAAAGCAACA CAAAAACAAG GTGAAATTGG CAGCAAACTT TTCACTTGCA 
CCTGTAACCA AGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014791860.1
CDS	159..5396
Translation	MMEGRRTENLCNRSFGWLQQQENDAKPWLWKLSNCFSAAEKSLPCSAETEDYMENKKAAAELKDASSPHNAGSKLFPAVPLPDVHPLQQQQIQLSPGPKVSCCAHGSDSSCTPQMHCGGGGGNLIHPGTILDSKSTGTITCQVGSGFAYQSASSLKNPPARSNLAGIASEFPGMCVENSVSSCQHLPCCGKLHFQSCHGNVHKLHQFPALQSCPSSGYFPCSEFPSGATGHLDEHIAQSELTSRVCANPLHLNMAPSVCLKGSHYCSDCLSKPTRNSLVDAAKIWPNIPPPSAQTAPVPIPICNGCGTKGTGNEKSLLLASSLGKSPQKYGSPEVAITGQVLENLPPIGVFWDIENCSVPTGRSAIAVVQRIREKFFKGHREAEFICVCDISKENKEVIQELNNCQVTVAHINATAKNAADDKLRQSLRRFADTHAAPATVVLVSTDVNFALELSDLRHRHGFRIILVHKNQASEALLHHAHELICFEEFISDLPPRLPLKMPACHTLLYVYNLPTNRDSKSISNRLRRLSDNCGGKVLSISGSSAILRFLNQESAERARKRMENEDVFGNRIVVSFTPKTKELSETKSSSFVGTEKVKSPKKINKNTKLCLLNKDSSDQSCGTKGSAGRGFQIHGSIIKPTNVKSLQELCRLESKTISRNTENQQELLREQIPSPQSNSNAAIPVSLAMKKTGMGESSCKSSQKRETSASRSVTNSPVDKKDKDETVFQVSYPSAFSKLTASRQLSPLLMSQSCWSSRSMSPNLSNRSSPLTFNVVNHTSGTDCPDPFANGADIHISNIDYRLSRKELQQNLHEIFSRHGKVKSVELSPHTDYQLKATIQMENLQEAISAVNSLHRYKIGSKRIQVSLATGAANKSLSLLSSETMSILQDAPACCLPVFKFTEIYEKKFGRKLILSDLYKLTDTVAIRDQGSGRLVCLLSSSQARQSPLGSSQSHDGSSANCSPIIFEELEYHEPVCKQHCLNKDFIEHEFDPDSYRIPFVILSLKTFAPQVHSLLQTHEGTVPLLSFPDCYMSEFNDLEIVPEGQGGVALEHLITCVPGVNIATAQNGIKVIKWIHNKPPPPTTDPWLLRSKSPVGNPQLIQFSREVIDLLKSQPSCVIPVSKFIPTYHHHFAKQCRVSDYGYSKLMELLEAVPHVLQILGMGSKRLLTLTHRAQVKRFTQDLLKLLKSQASKQVIVREFLQAYHWCFSKDWDVTEYGVCELADIISEIPDTTICLSQQDNEMVICIPKRERTQEEIERTKQFSKEVVDLLRHQPHFRMPFNKFIPSYHHHFGRQCKLAYYGFTKLLELFEAIPDVLHVLECGEEKILTLTEVEQVKAVAAQFVKLLRSQKDNCLMMADVLTEYSKTFGYTLRLHDYDVSSVPALMQKLCHVVKVVDTESGKQIQLINRKSLRTLTAQLLVLLMSWDGTSFLSVEQLKQHYETTHSTSLNPCEYGFMTLTELLKSLPYLVEVFTNGGAEEYVKLTNLYMFAKNVRSLLHTYHYQQIFLHEFPVAYSKYTGEVLQPKVYGCNNLEELLGAIPQVVWIKGHGHKRIVVLKNDMKTRFSSPSFPPADHVDDHGNQLADNNGHIMETPGSTSSMELSLGTPSNVSNPTEQELLCLTNTSPIDLLCEPVPSCLPSPQLRPDPVVLESADLIQFEERPAPLSEIMILTEEEKQRIGTTAQEKLTSGSVVSNTTENASVPPCQSSETQTHKEAMDSPAKKQHKNKVKLAANFSLAPVTKL

Target ORF information:

RefSeq Version	XM_014936374.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936374.1

ORF Insert Sequence:

ATGATGGAAG GGCGCAGGAC AGAGAACCTC TGCAATAGAT CGTTCGGATG GCTTCAGCAA 
CAAGAGAATG ATGCTAAACC ATGGCTCTGG AAACTTTCTA ATTGCTTTTC TGCTGCTGAG 
AAGTCTTTGC CTTGCAGTGC GGAAACGGAA GATTACATGG AGAACAAGAA AGCTGCTGCA 
GAATTGAAGG ATGCTTCATC TCCTCATAAT GCTGGCTCTA AACTGTTCCC AGCAGTACCG 
CTTCCTGATG TTCATCCTCT TCAGCAACAG CAAATACAGC TTTCACCTGG CCCGAAAGTA 
AGCTGCTGTG CTCATGGCTC TGACTCTTCT TGTACTCCAC AGATGCATTG TGGTGGTGGT 
GGTGGTAACT TGATTCACCC TGGCACAATA TTAGACTCAA AAAGCACTGG GACGATTACT 
TGTCAAGTAG GGTCAGGATT TGCTTATCAG TCTGCATCTT CACTGAAGAA CCCTCCAGCT 
AGAAGCAATT TGGCAGGCAT TGCGAGTGAG TTCCCTGGCA TGTGCGTAGA AAACAGTGTA 
TCTTCCTGTC AACATCTGCC CTGTTGTGGA AAACTCCATT TCCAGTCCTG TCATGGTAAT 
GTCCACAAAC TGCATCAGTT TCCAGCCCTT CAGAGCTGCC CATCTTCTGG CTATTTCCCT 
TGTTCTGAAT TCCCAAGTGG GGCCACAGGC CATTTGGATG AGCATATTGC ACAGTCGGAG 
CTAACATCGC GTGTGTGCGC CAACCCTTTG CACCTAAACA TGGCACCTTC AGTTTGTTTA 
AAGGGCTCTC ACTACTGCAG TGACTGCTTG AGCAAGCCAA CCAGAAACAG CCTAGTTGAT 
GCTGCTAAAA TCTGGCCAAA TATTCCTCCT CCAAGTGCAC AGACTGCACC TGTTCCTATC 
CCAATATGTA ATGGCTGTGG AACCAAAGGA ACAGGAAATG AGAAGAGTTT GCTACTGGCG 
AGCAGCCTTG GCAAATCACC GCAGAAATAT GGATCTCCGG AAGTTGCAAT AACAGGCCAA 
GTTCTGGAAA ACTTGCCCCC CATTGGAGTC TTCTGGGACA TTGAAAACTG TTCAGTTCCC 
ACTGGCCGTT CAGCTATAGC CGTTGTACAG AGGATACGTG AAAAGTTTTT TAAAGGTCAC 
AGAGAGGCAG AATTCATCTG TGTGTGTGAC ATTAGTAAAG AAAATAAAGA AGTTATTCAG 
GAGCTAAACA ACTGCCAGGT GACTGTTGCA CACATCAATG CTACAGCAAA GAATGCTGCT 
GATGACAAAC TCAGACAGAG TCTTAGGAGA TTTGCGGATA CCCATGCTGC ACCTGCCACT 
GTGGTTCTTG TGTCAACGGA TGTAAACTTT GCTTTGGAAC TCAGTGACCT GAGACATCGA 
CATGGTTTTC GGATCATTTT GGTACATAAA AACCAAGCTT CAGAAGCACT CTTACATCAT 
GCTCATGAGC TTATTTGTTT TGAAGAATTT ATTTCAGACT TGCCACCAAG GTTACCACTG 
AAAATGCCGG CATGCCATAC ACTGTTATAT GTTTATAACC TACCAACAAA TAGAGACAGC 
AAAAGTATCA GCAATAGACT CAGGCGTTTG TCGGACAACT GTGGAGGGAA GGTGCTGAGC 
ATTTCTGGAA GCAGTGCAAT TCTCCGTTTT TTAAATCAAG AAAGTGCTGA ACGCGCTCGG 
AAGCGAATGG AAAATGAAGA TGTTTTTGGT AACAGGATTG TAGTGTCTTT TACTCCCAAA 
ACCAAGGAAC TTAGTGAGAC AAAAAGCTCC AGCTTTGTGG GAACAGAAAA GGTGAAGTCT 
CCCAAAAAAA TTAACAAGAA CACAAAGCTG TGCCTCCTCA ACAAAGACTC AAGTGATCAG 
TCTTGTGGTA CCAAAGGCTC TGCTGGAAGA GGATTCCAGA TTCATGGATC TATCATCAAA 
CCCACAAATG TTAAAAGTTT ACAGGAGCTG TGCCGTCTTG AATCAAAGAC CATCAGTAGA 
AATACTGAAA ACCAGCAAGA ACTTTTAAGA GAACAAATTC CTTCTCCTCA GAGTAACTCT 
AATGCAGCAA TTCCTGTGTC TCTGGCAATG AAAAAAACTG GAATGGGAGA ATCATCCTGT 
AAAAGTAGTC AGAAAAGAGA GACTTCTGCT TCCAGGAGTG TTACCAATTC CCCTGTAGAT 
AAAAAAGATA AAGATGAAAC TGTGTTTCAG GTCAGCTATC CCTCTGCTTT CAGTAAGTTG 
ACGGCCTCAA GACAACTCAG TCCTTTACTC ATGTCTCAGA GTTGCTGGTC ATCTCGGAGT 
ATGTCTCCAA ACCTCTCAAA CAGATCATCT CCACTCACAT TTAATGTAGT AAATCATACC 
AGTGGTACAG ACTGCCCTGA TCCTTTTGCA AATGGTGCAG ACATTCACAT CAGCAATATA 
GATTACAGAT TGTCCAGAAA AGAGTTGCAG CAAAATTTGC ACGAAATTTT CTCAAGACAT 
GGCAAGGTAA AAAGTGTAGA GCTCAGTCCC CATACAGACT ACCAGTTAAA GGCTACAATT 
CAGATGGAAA ATCTGCAAGA AGCAATCAGT GCTGTTAACA GTCTTCACAG ATACAAAATT 
GGCAGTAAAA GGATCCAGGT CTCATTAGCA ACGGGAGCTG CTAATAAATC ACTCTCTCTC 
CTTAGCTCAG AAACAATGTC CATTCTGCAG GACGCACCTG CTTGTTGTCT GCCTGTGTTC 
AAATTCACAG AAATCTATGA AAAAAAATTT GGACGTAAGT TGATTCTATC GGATTTATAT 
AAATTAACGG ATACTGTGGC AATCCGTGAC CAAGGCAGCG GGCGGCTGGT GTGCCTCTTA 
TCCAGCAGCC AAGCCAGGCA GAGTCCATTG GGATCTTCAC AGTCACATGA TGGCTCCTCA 
GCAAACTGTA GTCCTATAAT ATTTGAAGAG TTGGAATATC ACGAGCCTGT TTGTAAGCAG 
CATTGCCTGA ATAAAGACTT TATTGAGCAT GAGTTTGATC CAGATTCTTA TAGAATTCCA 
TTTGTGATTT TGTCTCTGAA GACATTTGCT CCCCAAGTTC ATAGTCTTCT ACAGACACAT 
GAGGGTACTG TGCCTTTACT AAGTTTTCCT GATTGTTATA TGTCAGAGTT CAACGATCTT 
GAAATAGTGC CAGAAGGCCA GGGTGGTGTT GCTTTAGAAC ATCTAATTAC CTGTGTTCCT 
GGAGTTAACA TTGCCACTGC CCAGAACGGC ATTAAAGTTA TTAAATGGAT ACATAACAAA 
CCACCACCTC CTACTACAGA TCCTTGGCTT CTACGTTCTA AGAGCCCTGT GGGTAACCCA 
CAACTTATTC AGTTCAGTAG AGAAGTTATA GATCTACTTA AAAGCCAACC GTCCTGCGTC 
ATACCTGTCA GTAAATTCAT CCCAACGTAC CACCATCACT TCGCAAAGCA ATGCCGGGTG 
TCTGACTACG GGTATTCTAA ATTAATGGAG CTGCTGGAAG CAGTGCCTCA TGTGCTGCAA 
ATCCTTGGTA TGGGTTCCAA GCGCTTGTTG ACTCTAACAC ACAGAGCTCA AGTGAAGCGG 
TTTACTCAAG ACTTACTGAA GCTTCTCAAG TCCCAGGCCA GCAAGCAAGT TATTGTGAGG 
GAGTTCTTAC AGGCTTACCA CTGGTGTTTC TCTAAGGACT GGGATGTTAC TGAGTATGGA 
GTGTGTGAAC TGGCTGATAT TATATCAGAA ATTCCAGATA CAACTATCTG TTTGTCACAG 
CAAGACAATG AAATGGTAAT TTGTATCCCC AAAAGAGAAC GTACACAAGA AGAAATTGAA 
AGAACCAAAC AATTTTCTAA AGAGGTAGTA GACTTACTGC GCCACCAACC CCATTTTCGA 
ATGCCCTTCA ATAAATTTAT TCCTTCATAT CACCACCACT TTGGTCGTCA GTGCAAACTT 
GCTTACTATG GTTTTACAAA ACTACTTGAA CTCTTTGAAG CCATACCAGA TGTCTTACAT 
GTATTGGAAT GTGGGGAAGA GAAAATTCTC ACGCTCACAG AGGTGGAACA AGTCAAAGCA 
GTTGCTGCCC AGTTTGTTAA ACTGCTGCGG TCTCAGAAAG ACAACTGCCT TATGATGGCT 
GATGTGCTGA CAGAGTACAG CAAAACCTTT GGATACACGC TGCGCCTTCA TGACTACGAT 
GTTAGCTCAG TTCCAGCTTT AATGCAAAAA CTCTGCCACG TTGTAAAGGT TGTTGACACA 
GAGTCTGGCA AGCAAATTCA GCTGATAAAT AGAAAATCTC TGCGGACTCT GACTGCCCAA 
TTGCTAGTCT TGTTGATGTC CTGGGATGGA ACATCCTTTC TCTCTGTTGA ACAGCTTAAA 
CAGCATTATG AAACAACCCA TAGTACTTCC CTTAATCCTT GTGAATATGG ATTTATGACC 
TTAACTGAAC TCCTCAAGAG TCTGCCTTAC TTGGTTGAGG TTTTTACCAA TGGTGGAGCA 
GAAGAATATG TGAAGCTTAC AAATCTGTAT ATGTTTGCAA AGAATGTAAG GTCCTTACTT 
CACACCTACC ATTATCAGCA GATTTTTCTT CATGAGTTCC CAGTAGCATA TAGCAAATAC 
ACAGGAGAAG TGCTGCAACC TAAAGTATAT GGATGCAATA ATTTAGAAGA GCTTTTGGGA 
GCAATTCCAC AGGTGGTCTG GATTAAAGGA CATGGCCATA AAAGAATAGT GGTACTAAAG 
AATGATATGA AAACTCGTTT TAGCTCACCT AGTTTTCCCC CTGCTGATCA TGTGGATGAT 
CATGGAAATC AACTTGCTGA CAATAATGGA CATATTATGG AGACCCCAGG ATCTACTTCA 
TCAATGGAAC TAAGTCTAGG AACACCGAGC AATGTTTCTA ACCCAACTGA GCAAGAACTA 
CTTTGCCTCA CAAACACATC TCCCATTGAC CTCCTGTGTG AGCCAGTTCC TTCCTGCCTA 
CCGTCCCCAC AGCTGAGACC TGATCCGGTG GTTCTTGAGT CTGCAGACCT CATTCAGTTT 
GAGGAACGCC CTGCACCTCT CTCTGAGATA ATGATTTTAA CAGAAGAAGA AAAACAAAGA 
ATTGGCACCA CAGCTCAAGA AAAATTGACC TCTGGTTCCG TGGTATCTAA CACCACGGAG 
AATGCTTCAG TGCCTCCCTG CCAGTCCTCT GAAACCCAAA CCCACAAGGA AGCAATGGAC 
AGCCCAGCCA AAAAGCAACA CAAAAACAAG GTGAAATTGG CAGCAAACTT TTCACTTGCA 
CCTGTAACCA AGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA0430 ( XM_014936377.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014791863.1 Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X4, mRNA.

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CloneID	OCa60900
Clone ID Related Accession (Same CDS sequence)	XM_014936377.1
Accession Version	XM_014936377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	meiosis arrest female protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090794.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG GGCGCAGGAC AGAGAACCTC TGCAATAGAT CGTTCGGATG GCTTCAGCAA 
CAAGAGAATG ATGCTAAACC ATGGCTCTGG AAACTTTCTA ATTGCTTTTC TGCTGCTGAG 
AAGTCTTTGC CTTGCAGTGC GGAAACGGAA GATTACATGG AGAACAAGAA AGCTGCTGCA 
GAATTGAAGG ATGCTTCATC TCCTCATAAT GCTGGCTCTA AACTGTTCCC AGCAGTACCG 
CTTCCTGATG TTCATCCTCT TCAGCAACAG CAAATACAGC TTTCACCTGG CCCGAAACCA 
ACCAGAAACA GCCTAGTTGA TGCTGCTAAA ATCTGGCCAA ATATTCCTCC TCCAAGTGCA 
CAGACTGCAC CTGTTCCTAT CCCAATATGT AATGGCTGTG GAACCAAAGG AACAGGAAAT 
GAGAAGAGTT TGCTACTGGC GAGCAGCCTT GGCAAATCAC CGCAGAAATA TGGATCTCCG 
GAAGTTGCAA TAACAGGCCA AGTTCTGGAA AACTTGCCCC CCATTGGAGT CTTCTGGGAC 
ATTGAAAACT GTTCAGTTCC CACTGGCCGT TCAGCTATAG CCGTTGTACA GAGGATACGT 
GAAAAGTTTT TTAAAGGTCA CAGAGAGGCA GAATTCATCT GTGTGTGTGA CATTAGTAAA 
GAAAATAAAG AAGTTATTCA GGAGCTAAAC AACTGCCAGG TGACTGTTGC ACACATCAAT 
GCTACAGCAA AGAATGCTGC TGATGACAAA CTCAGACAGA GTCTTAGGAG ATTTGCGGAT 
ACCCATGCTG CACCTGCCAC TGTGGTTCTT GTGTCAACGG ATGTAAACTT TGCTTTGGAA 
CTCAGTGACC TGAGACATCG ACATGGTTTT CGGATCATTT TGGTACATAA AAACCAAGCT 
TCAGAAGCAC TCTTACATCA TGCTCATGAG CTTATTTGTT TTGAAGAATT TATTTCAGAC 
TTGCCACCAA GGTTACCACT GAAAATGCCG GCATGCCATA CACTGTTATA TGTTTATAAC 
CTACCAACAA ATAGAGACAG CAAAAGTATC AGCAATAGAC TCAGGCGTTT GTCGGACAAC 
TGTGGAGGGA AGGTGCTGAG CATTTCTGGA AGCAGTGCAA TTCTCCGTTT TTTAAATCAA 
GAAAGTGCTG AACGCGCTCG GAAGCGAATG GAAAATGAAG ATGTTTTTGG TAACAGGATT 
GTAGTGTCTT TTACTCCCAA AACCAAGGAA CTTAGTGAGA CAAAAAGCTC CAGCTTTGTG 
GGAACAGAAA AGGTGAAGTC TCCCAAAAAA ATTAACAAGA ACACAAAGCT GTGCCTCCTC 
AACAAAGACT CAAGTGATCA GTCTTGTGGT ACCAAAGGCT CTGCTGGAAG AGGATTCCAG 
ATTCATGGAT CTATCATCAA ACCCACAAAT GTTAAAAGTT TACAGGAGCT GTGCCGTCTT 
GAATCAAAGA CCATCAGTAG AAATACTGAA AACCAGCAAG AACTTTTAAG AGAACAAATT 
CCTTCTCCTC AGAGTAACTC TAATGCAGCA ATTCCTGTGT CTCTGGCAAT GAAAAAAACT 
GGAATGGGAG AATCATCCTG TAAAAGTAGT CAGAAAAGAG AGACTTCTGC TTCCAGGAGT 
GTTACCAATT CCCCTGTAGA TAAAAAAGAT AAAGATGAAA CTGTGTTTCA GGTCAGCTAT 
CCCTCTGCTT TCAGTAAGTT GACGGCCTCA AGACAACTCA GTCCTTTACT CATGTCTCAG 
AGTTGCTGGT CATCTCGGAG TATGTCTCCA AACCTCTCAA ACAGATCATC TCCACTCACA 
TTTAATGTAG TAAATCATAC CAGTGGTACA GACTGCCCTG ATCCTTTTGC AAATGGTGCA 
GACATTCACA TCAGCAATAT AGATTACAGA TTGTCCAGAA AAGAGTTGCA GCAAAATTTG 
CACGAAATTT TCTCAAGACA TGGCAAGGTA AAAAGTGTAG AGCTCAGTCC CCATACAGAC 
TACCAGTTAA AGGCTACAAT TCAGATGGAA AATCTGCAAG AAGCAATCAG TGCTGTTAAC 
AGTCTTCACA GATACAAAAT TGGCAGTAAA AGGATCCAGG TCTCATTAGC AACGGGAGCT 
GCTAATAAAT CACTCTCTCT CCTTAGCTCA GAAACAATGT CCATTCTGCA GGACGCACCT 
GCTTGTTGTC TGCCTGTGTT CAAATTCACA GAAATCTATG AAAAAAAATT TGGACGTAAG 
TTGATTCTAT CGGATTTATA TAAATTAACG GATACTGTGG CAATCCGTGA CCAAGGCAGC 
GGGCGGCTGG TGTGCCTCTT ATCCAGCAGC CAAGCCAGGC AGAGTCCATT GGGATCTTCA 
CAGTCACATG ATGGCTCCTC AGCAAACTGT AGTCCTATAA TATTTGAAGA GTTGGAATAT 
CACGAGCCTG TTTGTAAGCA GCATTGCCTG AATAAAGACT TTATTGAGCA TGAGTTTGAT 
CCAGATTCTT ATAGAATTCC ATTTGTGATT TTGTCTCTGA AGACATTTGC TCCCCAAGTT 
CATAGTCTTC TACAGACACA TGAGGGTACT GTGCCTTTAC TAAGTTTTCC TGATTGTTAT 
ATGTCAGAGT TCAACGATCT TGAAATAGTG CCAGAAGGCC AGGGTGGTGT TGCTTTAGAA 
CATCTAATTA CCTGTGTTCC TGGAGTTAAC ATTGCCACTG CCCAGAACGG CATTAAAGTT 
ATTAAATGGA TACATAACAA ACCACCACCT CCTACTACAG ATCCTTGGCT TCTACGTTCT 
AAGAGCCCTG TGGGTAACCC ACAACTTATT CAGTTCAGTA GAGAAGTTAT AGATCTACTT 
AAAAGCCAAC CGTCCTGCGT CATACCTGTC AGTAAATTCA TCCCAACGTA CCACCATCAC 
TTCGCAAAGC AATGCCGGGT GTCTGACTAC GGGTATTCTA AATTAATGGA GCTGCTGGAA 
GCAGTGCCTC ATGTGCTGCA AATCCTTGGT ATGGGTTCCA AGCGCTTGTT GACTCTAACA 
CACAGAGCTC AAGTGAAGCG GTTTACTCAA GACTTACTGA AGCTTCTCAA GTCCCAGGCC 
AGCAAGCAAG TTATTGTGAG GGAGTTCTTA CAGGCTTACC ACTGGTGTTT CTCTAAGGAC 
TGGGATGTTA CTGAGTATGG AGTGTGTGAA CTGGCTGATA TTATATCAGA AATTCCAGAT 
ACAACTATCT GTTTGTCACA GCAAGACAAT GAAATGGTAA TTTGTATCCC CAAAAGAGAA 
CGTACACAAG AAGAAATTGA AAGAACCAAA CAATTTTCTA AAGAGGTAGT AGACTTACTG 
CGCCACCAAC CCCATTTTCG AATGCCCTTC AATAAATTTA TTCCTTCATA TCACCACCAC 
TTTGGTCGTC AGTGCAAACT TGCTTACTAT GGTTTTACAA AACTACTTGA ACTCTTTGAA 
GCCATACCAG ATGTCTTACA TGTATTGGAA TGTGGGGAAG AGAAAATTCT CACGCTCACA 
GAGGTGGAAC AAGTCAAAGC AGTTGCTGCC CAGTTTGTTA AACTGCTGCG GTCTCAGAAA 
GACAACTGCC TTATGATGGC TGATGTGCTG ACAGAGTACA GCAAAACCTT TGGATACACG 
CTGCGCCTTC ATGACTACGA TGTTAGCTCA GTTCCAGCTT TAATGCAAAA ACTCTGCCAC 
GTTGTAAAGG TTGTTGACAC AGAGTCTGGC AAGCAAATTC AGCTGATAAA TAGAAAATCT 
CTGCGGACTC TGACTGCCCA ATTGCTAGTC TTGTTGATGT CCTGGGATGG AACATCCTTT 
CTCTCTGTTG AACAGCTTAA ACAGCATTAT GAAACAACCC ATAGTACTTC CCTTAATCCT 
TGTGAATATG GATTTATGAC CTTAACTGAA CTCCTCAAGA GTCTGCCTTA CTTGGTTGAG 
GTTTTTACCA ATGGTGGAGC AGAAGAATAT GTGAAGCTTA CAAATCTGTA TATGTTTGCA 
AAGAATGTAA GGTCCTTACT TCACACCTAC CATTATCAGC AGATTTTTCT TCATGAGTTC 
CCAGTAGCAT ATAGCAAATA CACAGGAGAA GTGCTGCAAC CTAAAGTATA TGGATGCAAT 
AATTTAGAAG AGCTTTTGGG AGCAATTCCA CAGGTGGTCT GGATTAAAGG ACATGGCCAT 
AAAAGAATAG TGGTACTAAA GAATGATATG AAAACTCGTT TTAGCTCACC TAGTTTTCCC 
CCTGCTGATC ATGTGGATGA TCATGGAAAT CAACTTGCTG ACAATAATGG ACATATTATG 
GAGACCCCAG GATCTACTTC ATCAATGGAA CTAAGTCTAG GAACACCGAG CAATGTTTCT 
AACCCAACTG AGCAAGAACT ACTTTGCCTC ACAAACACAT CTCCCATTGA CCTCCTGTGT 
GAGCCAGTTC CTTCCTGCCT ACCGTCCCCA CAGCTGAGAC CTGATCCGGT GGTTCTTGAG 
TCTGCAGACC TCATTCAGTT TGAGGAACGC CCTGCACCTC TCTCTGAGAT AATGATTTTA 
ACAGAAGAAG AAAAACAAAG AATTGGCACC ACAGCTCAAG AAAAATTGAC CTCTGGTTCC 
GTGGTATCTA ACACCACGGA GAATGCTTCA GTGCCTCCCT GCCAGTCCTC TGAAACCCAA 
ACCCACAAGG AAGCAATGGA CAGCCCAGCC AAAAAGCAAC ACAAAAACAA GGTGAAATTG 
GCAGCAAACT TTTCACTTGC ACCTGTAACC AAGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014791863.1
CDS	149..4867
Translation	MMEGRRTENLCNRSFGWLQQQENDAKPWLWKLSNCFSAAEKSLPCSAETEDYMENKKAAAELKDASSPHNAGSKLFPAVPLPDVHPLQQQQIQLSPGPKPTRNSLVDAAKIWPNIPPPSAQTAPVPIPICNGCGTKGTGNEKSLLLASSLGKSPQKYGSPEVAITGQVLENLPPIGVFWDIENCSVPTGRSAIAVVQRIREKFFKGHREAEFICVCDISKENKEVIQELNNCQVTVAHINATAKNAADDKLRQSLRRFADTHAAPATVVLVSTDVNFALELSDLRHRHGFRIILVHKNQASEALLHHAHELICFEEFISDLPPRLPLKMPACHTLLYVYNLPTNRDSKSISNRLRRLSDNCGGKVLSISGSSAILRFLNQESAERARKRMENEDVFGNRIVVSFTPKTKELSETKSSSFVGTEKVKSPKKINKNTKLCLLNKDSSDQSCGTKGSAGRGFQIHGSIIKPTNVKSLQELCRLESKTISRNTENQQELLREQIPSPQSNSNAAIPVSLAMKKTGMGESSCKSSQKRETSASRSVTNSPVDKKDKDETVFQVSYPSAFSKLTASRQLSPLLMSQSCWSSRSMSPNLSNRSSPLTFNVVNHTSGTDCPDPFANGADIHISNIDYRLSRKELQQNLHEIFSRHGKVKSVELSPHTDYQLKATIQMENLQEAISAVNSLHRYKIGSKRIQVSLATGAANKSLSLLSSETMSILQDAPACCLPVFKFTEIYEKKFGRKLILSDLYKLTDTVAIRDQGSGRLVCLLSSSQARQSPLGSSQSHDGSSANCSPIIFEELEYHEPVCKQHCLNKDFIEHEFDPDSYRIPFVILSLKTFAPQVHSLLQTHEGTVPLLSFPDCYMSEFNDLEIVPEGQGGVALEHLITCVPGVNIATAQNGIKVIKWIHNKPPPPTTDPWLLRSKSPVGNPQLIQFSREVIDLLKSQPSCVIPVSKFIPTYHHHFAKQCRVSDYGYSKLMELLEAVPHVLQILGMGSKRLLTLTHRAQVKRFTQDLLKLLKSQASKQVIVREFLQAYHWCFSKDWDVTEYGVCELADIISEIPDTTICLSQQDNEMVICIPKRERTQEEIERTKQFSKEVVDLLRHQPHFRMPFNKFIPSYHHHFGRQCKLAYYGFTKLLELFEAIPDVLHVLECGEEKILTLTEVEQVKAVAAQFVKLLRSQKDNCLMMADVLTEYSKTFGYTLRLHDYDVSSVPALMQKLCHVVKVVDTESGKQIQLINRKSLRTLTAQLLVLLMSWDGTSFLSVEQLKQHYETTHSTSLNPCEYGFMTLTELLKSLPYLVEVFTNGGAEEYVKLTNLYMFAKNVRSLLHTYHYQQIFLHEFPVAYSKYTGEVLQPKVYGCNNLEELLGAIPQVVWIKGHGHKRIVVLKNDMKTRFSSPSFPPADHVDDHGNQLADNNGHIMETPGSTSSMELSLGTPSNVSNPTEQELLCLTNTSPIDLLCEPVPSCLPSPQLRPDPVVLESADLIQFEERPAPLSEIMILTEEEKQRIGTTAQEKLTSGSVVSNTTENASVPPCQSSETQTHKEAMDSPAKKQHKNKVKLAANFSLAPVTKL

Target ORF information:

RefSeq Version	XM_014936377.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax KIAA0430 ortholog (KIAA0430), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936377.1

ORF Insert Sequence:

ATGATGGAAG GGCGCAGGAC AGAGAACCTC TGCAATAGAT CGTTCGGATG GCTTCAGCAA 
CAAGAGAATG ATGCTAAACC ATGGCTCTGG AAACTTTCTA ATTGCTTTTC TGCTGCTGAG 
AAGTCTTTGC CTTGCAGTGC GGAAACGGAA GATTACATGG AGAACAAGAA AGCTGCTGCA 
GAATTGAAGG ATGCTTCATC TCCTCATAAT GCTGGCTCTA AACTGTTCCC AGCAGTACCG 
CTTCCTGATG TTCATCCTCT TCAGCAACAG CAAATACAGC TTTCACCTGG CCCGAAACCA 
ACCAGAAACA GCCTAGTTGA TGCTGCTAAA ATCTGGCCAA ATATTCCTCC TCCAAGTGCA 
CAGACTGCAC CTGTTCCTAT CCCAATATGT AATGGCTGTG GAACCAAAGG AACAGGAAAT 
GAGAAGAGTT TGCTACTGGC GAGCAGCCTT GGCAAATCAC CGCAGAAATA TGGATCTCCG 
GAAGTTGCAA TAACAGGCCA AGTTCTGGAA AACTTGCCCC CCATTGGAGT CTTCTGGGAC 
ATTGAAAACT GTTCAGTTCC CACTGGCCGT TCAGCTATAG CCGTTGTACA GAGGATACGT 
GAAAAGTTTT TTAAAGGTCA CAGAGAGGCA GAATTCATCT GTGTGTGTGA CATTAGTAAA 
GAAAATAAAG AAGTTATTCA GGAGCTAAAC AACTGCCAGG TGACTGTTGC ACACATCAAT 
GCTACAGCAA AGAATGCTGC TGATGACAAA CTCAGACAGA GTCTTAGGAG ATTTGCGGAT 
ACCCATGCTG CACCTGCCAC TGTGGTTCTT GTGTCAACGG ATGTAAACTT TGCTTTGGAA 
CTCAGTGACC TGAGACATCG ACATGGTTTT CGGATCATTT TGGTACATAA AAACCAAGCT 
TCAGAAGCAC TCTTACATCA TGCTCATGAG CTTATTTGTT TTGAAGAATT TATTTCAGAC 
TTGCCACCAA GGTTACCACT GAAAATGCCG GCATGCCATA CACTGTTATA TGTTTATAAC 
CTACCAACAA ATAGAGACAG CAAAAGTATC AGCAATAGAC TCAGGCGTTT GTCGGACAAC 
TGTGGAGGGA AGGTGCTGAG CATTTCTGGA AGCAGTGCAA TTCTCCGTTT TTTAAATCAA 
GAAAGTGCTG AACGCGCTCG GAAGCGAATG GAAAATGAAG ATGTTTTTGG TAACAGGATT 
GTAGTGTCTT TTACTCCCAA AACCAAGGAA CTTAGTGAGA CAAAAAGCTC CAGCTTTGTG 
GGAACAGAAA AGGTGAAGTC TCCCAAAAAA ATTAACAAGA ACACAAAGCT GTGCCTCCTC 
AACAAAGACT CAAGTGATCA GTCTTGTGGT ACCAAAGGCT CTGCTGGAAG AGGATTCCAG 
ATTCATGGAT CTATCATCAA ACCCACAAAT GTTAAAAGTT TACAGGAGCT GTGCCGTCTT 
GAATCAAAGA CCATCAGTAG AAATACTGAA AACCAGCAAG AACTTTTAAG AGAACAAATT 
CCTTCTCCTC AGAGTAACTC TAATGCAGCA ATTCCTGTGT CTCTGGCAAT GAAAAAAACT 
GGAATGGGAG AATCATCCTG TAAAAGTAGT CAGAAAAGAG AGACTTCTGC TTCCAGGAGT 
GTTACCAATT CCCCTGTAGA TAAAAAAGAT AAAGATGAAA CTGTGTTTCA GGTCAGCTAT 
CCCTCTGCTT TCAGTAAGTT GACGGCCTCA AGACAACTCA GTCCTTTACT CATGTCTCAG 
AGTTGCTGGT CATCTCGGAG TATGTCTCCA AACCTCTCAA ACAGATCATC TCCACTCACA 
TTTAATGTAG TAAATCATAC CAGTGGTACA GACTGCCCTG ATCCTTTTGC AAATGGTGCA 
GACATTCACA TCAGCAATAT AGATTACAGA TTGTCCAGAA AAGAGTTGCA GCAAAATTTG 
CACGAAATTT TCTCAAGACA TGGCAAGGTA AAAAGTGTAG AGCTCAGTCC CCATACAGAC 
TACCAGTTAA AGGCTACAAT TCAGATGGAA AATCTGCAAG AAGCAATCAG TGCTGTTAAC 
AGTCTTCACA GATACAAAAT TGGCAGTAAA AGGATCCAGG TCTCATTAGC AACGGGAGCT 
GCTAATAAAT CACTCTCTCT CCTTAGCTCA GAAACAATGT CCATTCTGCA GGACGCACCT 
GCTTGTTGTC TGCCTGTGTT CAAATTCACA GAAATCTATG AAAAAAAATT TGGACGTAAG 
TTGATTCTAT CGGATTTATA TAAATTAACG GATACTGTGG CAATCCGTGA CCAAGGCAGC 
GGGCGGCTGG TGTGCCTCTT ATCCAGCAGC CAAGCCAGGC AGAGTCCATT GGGATCTTCA 
CAGTCACATG ATGGCTCCTC AGCAAACTGT AGTCCTATAA TATTTGAAGA GTTGGAATAT 
CACGAGCCTG TTTGTAAGCA GCATTGCCTG AATAAAGACT TTATTGAGCA TGAGTTTGAT 
CCAGATTCTT ATAGAATTCC ATTTGTGATT TTGTCTCTGA AGACATTTGC TCCCCAAGTT 
CATAGTCTTC TACAGACACA TGAGGGTACT GTGCCTTTAC TAAGTTTTCC TGATTGTTAT 
ATGTCAGAGT TCAACGATCT TGAAATAGTG CCAGAAGGCC AGGGTGGTGT TGCTTTAGAA 
CATCTAATTA CCTGTGTTCC TGGAGTTAAC ATTGCCACTG CCCAGAACGG CATTAAAGTT 
ATTAAATGGA TACATAACAA ACCACCACCT CCTACTACAG ATCCTTGGCT TCTACGTTCT 
AAGAGCCCTG TGGGTAACCC ACAACTTATT CAGTTCAGTA GAGAAGTTAT AGATCTACTT 
AAAAGCCAAC CGTCCTGCGT CATACCTGTC AGTAAATTCA TCCCAACGTA CCACCATCAC 
TTCGCAAAGC AATGCCGGGT GTCTGACTAC GGGTATTCTA AATTAATGGA GCTGCTGGAA 
GCAGTGCCTC ATGTGCTGCA AATCCTTGGT ATGGGTTCCA AGCGCTTGTT GACTCTAACA 
CACAGAGCTC AAGTGAAGCG GTTTACTCAA GACTTACTGA AGCTTCTCAA GTCCCAGGCC 
AGCAAGCAAG TTATTGTGAG GGAGTTCTTA CAGGCTTACC ACTGGTGTTT CTCTAAGGAC 
TGGGATGTTA CTGAGTATGG AGTGTGTGAA CTGGCTGATA TTATATCAGA AATTCCAGAT 
ACAACTATCT GTTTGTCACA GCAAGACAAT GAAATGGTAA TTTGTATCCC CAAAAGAGAA 
CGTACACAAG AAGAAATTGA AAGAACCAAA CAATTTTCTA AAGAGGTAGT AGACTTACTG 
CGCCACCAAC CCCATTTTCG AATGCCCTTC AATAAATTTA TTCCTTCATA TCACCACCAC 
TTTGGTCGTC AGTGCAAACT TGCTTACTAT GGTTTTACAA AACTACTTGA ACTCTTTGAA 
GCCATACCAG ATGTCTTACA TGTATTGGAA TGTGGGGAAG AGAAAATTCT CACGCTCACA 
GAGGTGGAAC AAGTCAAAGC AGTTGCTGCC CAGTTTGTTA AACTGCTGCG GTCTCAGAAA 
GACAACTGCC TTATGATGGC TGATGTGCTG ACAGAGTACA GCAAAACCTT TGGATACACG 
CTGCGCCTTC ATGACTACGA TGTTAGCTCA GTTCCAGCTT TAATGCAAAA ACTCTGCCAC 
GTTGTAAAGG TTGTTGACAC AGAGTCTGGC AAGCAAATTC AGCTGATAAA TAGAAAATCT 
CTGCGGACTC TGACTGCCCA ATTGCTAGTC TTGTTGATGT CCTGGGATGG AACATCCTTT 
CTCTCTGTTG AACAGCTTAA ACAGCATTAT GAAACAACCC ATAGTACTTC CCTTAATCCT 
TGTGAATATG GATTTATGAC CTTAACTGAA CTCCTCAAGA GTCTGCCTTA CTTGGTTGAG 
GTTTTTACCA ATGGTGGAGC AGAAGAATAT GTGAAGCTTA CAAATCTGTA TATGTTTGCA 
AAGAATGTAA GGTCCTTACT TCACACCTAC CATTATCAGC AGATTTTTCT TCATGAGTTC 
CCAGTAGCAT ATAGCAAATA CACAGGAGAA GTGCTGCAAC CTAAAGTATA TGGATGCAAT 
AATTTAGAAG AGCTTTTGGG AGCAATTCCA CAGGTGGTCT GGATTAAAGG ACATGGCCAT 
AAAAGAATAG TGGTACTAAA GAATGATATG AAAACTCGTT TTAGCTCACC TAGTTTTCCC 
CCTGCTGATC ATGTGGATGA TCATGGAAAT CAACTTGCTG ACAATAATGG ACATATTATG 
GAGACCCCAG GATCTACTTC ATCAATGGAA CTAAGTCTAG GAACACCGAG CAATGTTTCT 
AACCCAACTG AGCAAGAACT ACTTTGCCTC ACAAACACAT CTCCCATTGA CCTCCTGTGT 
GAGCCAGTTC CTTCCTGCCT ACCGTCCCCA CAGCTGAGAC CTGATCCGGT GGTTCTTGAG 
TCTGCAGACC TCATTCAGTT TGAGGAACGC CCTGCACCTC TCTCTGAGAT AATGATTTTA 
ACAGAAGAAG AAAAACAAAG AATTGGCACC ACAGCTCAAG AAAAATTGAC CTCTGGTTCC 
GTGGTATCTA ACACCACGGA GAATGCTTCA GTGCCTCCCT GCCAGTCCTC TGAAACCCAA 
ACCCACAAGG AAGCAATGGA CAGCCCAGCC AAAAAGCAAC ACAAAAACAA GGTGAAATTG 
GCAGCAAACT TTTCACTTGC ACCTGTAACC AAGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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KIAA0430 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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