SMURF1 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol SMURF1

Entrez Gene ID 106884872

Full Name SMAD specific E3 ubiquitin protein ligase 1

Gene Type protein-coding

Organism Calidris pugnax(ruff)

mRNA	Protein	Name
XM_014936601.1	XP_014792087.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_014936602.1	XP_014792088.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_014936604.1	XP_014792090.1	E3 ubiquitin-protein ligase SMURF1 isoform X4
XM_014936603.1	XP_014792089.1	E3 ubiquitin-protein ligase SMURF1 isoform X3

Pathways from BioSystems
Gene Ontology

The following SMURF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMURF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa61067	XM_014936601.1 Latest version!	Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa61068	XM_014936602.1 Latest version!	Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa61069	XM_014936603.1 Latest version!	Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa61070	XM_014936604.1 Latest version!	Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMURF1 ( XM_014936601.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014792087.1 Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa61067
Clone ID Related Accession (Same CDS sequence)	XM_014936601.1
Accession Version	XM_014936601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090794.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGATTTCT TCAGACTTCC TGATCCCTTT 
GCAAAAATAG TTGTGGATGG GTCGGGTCAG TGCCATTCAA CTGACACTGT GAAGAACACC 
TTGGACCCCA AATGGAACCA GCATTATGAT CTATACGTTG GGAAGACAGA TTCCATAACC 
ATCAGCGTAT GGAACCATAA AAAAATCCAC AAGAAACAGG GAGCTGGCTT CCTGGGGTGT 
GTCCGACTCC TCTCCAATGC CATCAGCAGG CTAAAAGATA CTGGATACCA GCGTTTGGAT 
CTATGCAAAT TAAATCCCAC CGATACCGAT GCTGTACGAG GCCAAATAGT GGTCAGTTTA 
CAGACACGGG ACAGAATAGG TACAGGAGGT TCTGTAGTAG ACTGTAGAGG GCTTTTGGAG 
AATGAAGGTT TCCCCATTTT CAGAACGGTT TATGAAGATT CCGGACCGGG AAGGCCACTG 
AGCTGTTTTA TGGAGGAACC AGCACCGTAC ACGGACACGA CGGGGGCTGC TGGGGGAGGC 
AACTGTCGCT TCGTAGAGTC CCCCAGTCAA GACCAGAGGC TTCAGGCGCA GCGACTTCGG 
ACTCCTGAAG TCAGAGGACA CGTCCAGACA CCTCAGAACA GACCTCATGG TCACCAGTCG 
CCTGACCTAC CAGAAGGCTA CGAGCAAAGA ACAACGGTAC AGGGACAGGT TTATTTTTTG 
CACACACAGA CTGGAGTTAG CACGTGGCAT GACCCTAGGA TACCAAGAGA CCTTAACAGT 
GTGAATTGTG ATGAACTGGG ACCTTTGCCT CCAGGTTGGG AAGTTAGAAG TACAGTTTCA 
GGAAGAATAT ATTTTGTAGA TCATAACAAC AGAACCACAC AATTCACAGA TCCACGACTA 
CATCACATCA TGAACCATCA ATGCCAGCTT AAAGAGCCCA GCCAGCAGCC TTTGCCAGCT 
CCAAACGAGG GGTCGCTAGA AGACGGCGAG GAGTTGCCTG CACAAAGATA TGAAAGAGAC 
TTGGTACAAA AGTTGAAAGT TCTTAGACAT GAGCTCTCTC TTCAGCAACC ACAGGCTGGT 
CACTGTCGCA TTGAAGTATC CAGAGAAGAA ATATTTGAGG AATCCTACCG TCAGATAATG 
AAGATGAGGC CAAAGGACTT GAAAAAGAGG CTTATGGTGA AATTTCGCGG AGAGGAAGGC 
CTGGATTATG GAGGAGTAGC AAGAGAATGG TTGTATTTAC TGTGTCATGA AATGTTGAAT 
CCCTATTATG GACTCTTCCA GTACTCCACA GATAATATTT ACATGCTGCA AATCAATCCA 
GACTCTTCTA TCAATCCTGA CCATTTGTCT TATTTCCATT TTGTTGGTCG GATAATGGGT 
TTGGCTGTGT TCCATGGACA CTATATCAAC GGGGGGTTCA CGGTTCCCTT TTACAAACAG 
CTGCTGGGGA AGCCCATTCA GCTCTCCGAT CTGGAATCTG TTGACCCAGA ATTACACAAA 
AGTTTAGTCT GGATCTTAGA GAACGATATC ACCCCGGTTC TGGACCATAC GTTTTGTGTG 
GAACACAATG CTTTTGGGAG GATTTTACAG CATGAACTCA AACCAAATGG CAGGAATATT 
CCAGTGACAG AAGAGAACAA GAAAGAATAT GTCAGGTTGT ATGTAAACTG GCGATTTATG 
AGAGGAATAG AGGCGCAGTT CCTGGCTCTG CAGAAAGGTT TTAATGAGCT TATTCCTCAG 
CATCTCCTTA AGCCTTTCGA CCAGAAGGAA CTTGAGCTAA TCATAGGAGG CCTGGATAAG 
ATAGACCTGA ACGACTGGAA GTCCAACACC CGCCTAAAGC ACTGCATGGC AGACAGCAAC 
ATCGTGAAGT GGTTCTGGCA GGCAGTGGAA ACGTTTGATG AGGAGAGAAG GGCAAGGCTG 
CTGCAGTTTG TGACAGGCTC GACGCGTGTC CCACTTCAAG GTTTCAAGGC TTTACAAGGT 
TCTACAGGCG CTGCAGGACC CAGACTGTTT ACCATCCATT TAATAGATGC AAACACAGAC 
AACCTTCCAA AAGCCCACAC TTGCTTTAAC CGGATCGACA TTCCGCCTTA TGAGTCATAT 
GAGAAGCTTT ATGAGAAGCT GTTGACAGCT GTGGAAGAGA CGTGTGGGTT TGCTGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014792087.1
CDS	18..2180
Translation	MVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPTDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGFPIFRTVYEDSGPGRPLSCFMEEPAPYTDTTGAAGGGNCRFVESPSQDQRLQAQRLRTPEVRGHVQTPQNRPHGHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQQPLPAPNEGSLEDGEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCMADSNIVKWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_014936601.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936601.1

ORF Insert Sequence:

ATGGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGATTTCT TCAGACTTCC TGATCCCTTT 
GCAAAAATAG TTGTGGATGG GTCGGGTCAG TGCCATTCAA CTGACACTGT GAAGAACACC 
TTGGACCCCA AATGGAACCA GCATTATGAT CTATACGTTG GGAAGACAGA TTCCATAACC 
ATCAGCGTAT GGAACCATAA AAAAATCCAC AAGAAACAGG GAGCTGGCTT CCTGGGGTGT 
GTCCGACTCC TCTCCAATGC CATCAGCAGG CTAAAAGATA CTGGATACCA GCGTTTGGAT 
CTATGCAAAT TAAATCCCAC CGATACCGAT GCTGTACGAG GCCAAATAGT GGTCAGTTTA 
CAGACACGGG ACAGAATAGG TACAGGAGGT TCTGTAGTAG ACTGTAGAGG GCTTTTGGAG 
AATGAAGGTT TCCCCATTTT CAGAACGGTT TATGAAGATT CCGGACCGGG AAGGCCACTG 
AGCTGTTTTA TGGAGGAACC AGCACCGTAC ACGGACACGA CGGGGGCTGC TGGGGGAGGC 
AACTGTCGCT TCGTAGAGTC CCCCAGTCAA GACCAGAGGC TTCAGGCGCA GCGACTTCGG 
ACTCCTGAAG TCAGAGGACA CGTCCAGACA CCTCAGAACA GACCTCATGG TCACCAGTCG 
CCTGACCTAC CAGAAGGCTA CGAGCAAAGA ACAACGGTAC AGGGACAGGT TTATTTTTTG 
CACACACAGA CTGGAGTTAG CACGTGGCAT GACCCTAGGA TACCAAGAGA CCTTAACAGT 
GTGAATTGTG ATGAACTGGG ACCTTTGCCT CCAGGTTGGG AAGTTAGAAG TACAGTTTCA 
GGAAGAATAT ATTTTGTAGA TCATAACAAC AGAACCACAC AATTCACAGA TCCACGACTA 
CATCACATCA TGAACCATCA ATGCCAGCTT AAAGAGCCCA GCCAGCAGCC TTTGCCAGCT 
CCAAACGAGG GGTCGCTAGA AGACGGCGAG GAGTTGCCTG CACAAAGATA TGAAAGAGAC 
TTGGTACAAA AGTTGAAAGT TCTTAGACAT GAGCTCTCTC TTCAGCAACC ACAGGCTGGT 
CACTGTCGCA TTGAAGTATC CAGAGAAGAA ATATTTGAGG AATCCTACCG TCAGATAATG 
AAGATGAGGC CAAAGGACTT GAAAAAGAGG CTTATGGTGA AATTTCGCGG AGAGGAAGGC 
CTGGATTATG GAGGAGTAGC AAGAGAATGG TTGTATTTAC TGTGTCATGA AATGTTGAAT 
CCCTATTATG GACTCTTCCA GTACTCCACA GATAATATTT ACATGCTGCA AATCAATCCA 
GACTCTTCTA TCAATCCTGA CCATTTGTCT TATTTCCATT TTGTTGGTCG GATAATGGGT 
TTGGCTGTGT TCCATGGACA CTATATCAAC GGGGGGTTCA CGGTTCCCTT TTACAAACAG 
CTGCTGGGGA AGCCCATTCA GCTCTCCGAT CTGGAATCTG TTGACCCAGA ATTACACAAA 
AGTTTAGTCT GGATCTTAGA GAACGATATC ACCCCGGTTC TGGACCATAC GTTTTGTGTG 
GAACACAATG CTTTTGGGAG GATTTTACAG CATGAACTCA AACCAAATGG CAGGAATATT 
CCAGTGACAG AAGAGAACAA GAAAGAATAT GTCAGGTTGT ATGTAAACTG GCGATTTATG 
AGAGGAATAG AGGCGCAGTT CCTGGCTCTG CAGAAAGGTT TTAATGAGCT TATTCCTCAG 
CATCTCCTTA AGCCTTTCGA CCAGAAGGAA CTTGAGCTAA TCATAGGAGG CCTGGATAAG 
ATAGACCTGA ACGACTGGAA GTCCAACACC CGCCTAAAGC ACTGCATGGC AGACAGCAAC 
ATCGTGAAGT GGTTCTGGCA GGCAGTGGAA ACGTTTGATG AGGAGAGAAG GGCAAGGCTG 
CTGCAGTTTG TGACAGGCTC GACGCGTGTC CCACTTCAAG GTTTCAAGGC TTTACAAGGT 
TCTACAGGCG CTGCAGGACC CAGACTGTTT ACCATCCATT TAATAGATGC AAACACAGAC 
AACCTTCCAA AAGCCCACAC TTGCTTTAAC CGGATCGACA TTCCGCCTTA TGAGTCATAT 
GAGAAGCTTT ATGAGAAGCT GTTGACAGCT GTGGAAGAGA CGTGTGGGTT TGCTGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_014936602.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014792088.1 Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa61068
Clone ID Related Accession (Same CDS sequence)	XM_014936602.1
Accession Version	XM_014936602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090794.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGATTTCT TCAGACTTCC TGATCCCTTT 
GCAAAAATAG TTGTGGATGG GTCGGGTCAG TGCCATTCAA CTGACACTGT GAAGAACACC 
TTGGACCCCA AATGGAACCA GCATTATGAT CTATACGTTG GGAAGACAGA TTCCATAACC 
ATCAGCGTAT GGAACCATAA AAAAATCCAC AAGAAACAGG GAGCTGGCTT CCTGGGGTGT 
GTCCGACTCC TCTCCAATGC CATCAGCAGG CTAAAAGATA CTGGATACCA GCGTTTGGAT 
CTATGCAAAT TAAATCCCAC CGATACCGAT GCTGTACGAG GCCAAATAGT GGTCAGTTTA 
CAGACACGGG ACAGAATAGG TACAGGAGGT TCTGTAGTAG ACTGTAGAGG GCTTTTGGAG 
AATGAAGGTT TCCCCATTTT CAGAACGGTT TATGAAGATT CCGGACCGGG AAGGCCACTG 
AGCTGTTTTA TGGAGGAACC AGCACCGTAC ACGGACACGA CGGGGGCTGC TGGGGGAGGC 
AACTGTCGCT TCGTAGAGTC CCCCAGTCAA GACCAGAGGC TTCAGGCGCA GCGACTTCGG 
ACTCCTGAAG TCAGAGGACA CGTCCAGACA CCTCAGAACA GACCTCATGG TCACCAGTCG 
CCTGACCTAC CAGAAGGCTA CGAGCAAAGA ACAACGGTAC AGGGACAGGT TTATTTTTTG 
CACACACAGA CTGGAGTTAG CACGTGGCAT GACCCTAGGA TACCAAGAGA CCTTAACAGT 
GTGAATTGTG ATGAACTGGG ACCTTTGCCT CCAGGTTGGG AAGTTAGAAG TACAGTTTCA 
GGAAGAATAT ATTTTGTAGA TCATAACAAC AGAACCACAC AATTCACAGA TCCACGACTA 
CATCACATCA TGAACCATCA ATGCCAGCTT AAAGAGCCCA GCCAGCAGCC TTTGCCAGCT 
CCAAACGAGG GGTCGCTAGA AGACGGCGAG GAGTTGCCTG CACAAAGATA TGAAAGAGAC 
TTGGTACAAA AGTTGAAAGT TCTTAGACAT GAGCTCTCTC TTCAGCAACC ACAGGCTGGT 
CACTGTCGCA TTGAAGTATC CAGAGAAGAA ATATTTGAGG AATCCTACCG TCAGATAATG 
AAGATGAGGC CAAAGGACTT GAAAAAGAGG CTTATGGTGA AATTTCGCGG AGAGGAAGGC 
CTGGATTATG GAGGAGTAGC AAGAGAATGG TTGTATTTAC TGTGTCATGA AATGTTGAAT 
CCCTATTATG GACTCTTCCA GTACTCCACA GATAATATTT ACATGCTGCA AATCAATCCA 
GACTCTTCTA TCAATCCTGA CCATTTGTCT TATTTCCATT TTGTTGGTCG GATAATGGGT 
TTGGCTGTGT TCCATGGACA CTATATCAAC GGGGGGTTCA CGGTTCCCTT TTACAAACAG 
CTGCTGGGGA AGCCCATTCA GCTCTCCGAT CTGGAATCTG TTGACCCAGA ATTACACAAA 
AGTTTAGTCT GGATCTTAGA GAACGATATC ACCCCGGTTC TGGACCATAC GTTTTGTGTG 
GAACACAATG CTTTTGGGAG GATTTTACAG CATGAACTCA AACCAAATGG CAGGAATATT 
CCAGTGACAG AAGAGAACAA GAAAGAATAT GTCAGGTTGT ATGTAAACTG GCGATTTATG 
AGAGGAATAG AGGCGCAGTT CCTGGCTCTG CAGAAAGGTT TTAATGAGCT TATTCCTCAG 
CATCTCCTTA AGCCTTTCGA CCAGAAGGAA CTTGAGCTAA TCATAGGAGG CCTGGATAAG 
ATAGACCTGA ACGACTGGAA GTCCAACACC CGCCTAAAGC ACTGCATGGC AGACAGCAAC 
ATCGTGAAGT GGTTCTGGCA GGCAGTGGAA ACGTTTGATG AGGAGAGAAG GGCAAGGCTG 
CTGCAGTTTG TGACAGGCTC GACGCGTGTC CCACTTCAAG GTTTCAAGGC TTTACAAGGC 
GCTGCAGGAC CCAGACTGTT TACCATCCAT TTAATAGATG CAAACACAGA CAACCTTCCA 
AAAGCCCACA CTTGCTTTAA CCGGATCGAC ATTCCGCCTT ATGAGTCATA TGAGAAGCTT 
TATGAGAAGC TGTTGACAGC TGTGGAAGAG ACGTGTGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014792088.1
CDS	18..2171
Translation	MVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPTDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGFPIFRTVYEDSGPGRPLSCFMEEPAPYTDTTGAAGGGNCRFVESPSQDQRLQAQRLRTPEVRGHVQTPQNRPHGHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQQPLPAPNEGSLEDGEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCMADSNIVKWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_014936602.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936602.1

ORF Insert Sequence:

ATGGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGATTTCT TCAGACTTCC TGATCCCTTT 
GCAAAAATAG TTGTGGATGG GTCGGGTCAG TGCCATTCAA CTGACACTGT GAAGAACACC 
TTGGACCCCA AATGGAACCA GCATTATGAT CTATACGTTG GGAAGACAGA TTCCATAACC 
ATCAGCGTAT GGAACCATAA AAAAATCCAC AAGAAACAGG GAGCTGGCTT CCTGGGGTGT 
GTCCGACTCC TCTCCAATGC CATCAGCAGG CTAAAAGATA CTGGATACCA GCGTTTGGAT 
CTATGCAAAT TAAATCCCAC CGATACCGAT GCTGTACGAG GCCAAATAGT GGTCAGTTTA 
CAGACACGGG ACAGAATAGG TACAGGAGGT TCTGTAGTAG ACTGTAGAGG GCTTTTGGAG 
AATGAAGGTT TCCCCATTTT CAGAACGGTT TATGAAGATT CCGGACCGGG AAGGCCACTG 
AGCTGTTTTA TGGAGGAACC AGCACCGTAC ACGGACACGA CGGGGGCTGC TGGGGGAGGC 
AACTGTCGCT TCGTAGAGTC CCCCAGTCAA GACCAGAGGC TTCAGGCGCA GCGACTTCGG 
ACTCCTGAAG TCAGAGGACA CGTCCAGACA CCTCAGAACA GACCTCATGG TCACCAGTCG 
CCTGACCTAC CAGAAGGCTA CGAGCAAAGA ACAACGGTAC AGGGACAGGT TTATTTTTTG 
CACACACAGA CTGGAGTTAG CACGTGGCAT GACCCTAGGA TACCAAGAGA CCTTAACAGT 
GTGAATTGTG ATGAACTGGG ACCTTTGCCT CCAGGTTGGG AAGTTAGAAG TACAGTTTCA 
GGAAGAATAT ATTTTGTAGA TCATAACAAC AGAACCACAC AATTCACAGA TCCACGACTA 
CATCACATCA TGAACCATCA ATGCCAGCTT AAAGAGCCCA GCCAGCAGCC TTTGCCAGCT 
CCAAACGAGG GGTCGCTAGA AGACGGCGAG GAGTTGCCTG CACAAAGATA TGAAAGAGAC 
TTGGTACAAA AGTTGAAAGT TCTTAGACAT GAGCTCTCTC TTCAGCAACC ACAGGCTGGT 
CACTGTCGCA TTGAAGTATC CAGAGAAGAA ATATTTGAGG AATCCTACCG TCAGATAATG 
AAGATGAGGC CAAAGGACTT GAAAAAGAGG CTTATGGTGA AATTTCGCGG AGAGGAAGGC 
CTGGATTATG GAGGAGTAGC AAGAGAATGG TTGTATTTAC TGTGTCATGA AATGTTGAAT 
CCCTATTATG GACTCTTCCA GTACTCCACA GATAATATTT ACATGCTGCA AATCAATCCA 
GACTCTTCTA TCAATCCTGA CCATTTGTCT TATTTCCATT TTGTTGGTCG GATAATGGGT 
TTGGCTGTGT TCCATGGACA CTATATCAAC GGGGGGTTCA CGGTTCCCTT TTACAAACAG 
CTGCTGGGGA AGCCCATTCA GCTCTCCGAT CTGGAATCTG TTGACCCAGA ATTACACAAA 
AGTTTAGTCT GGATCTTAGA GAACGATATC ACCCCGGTTC TGGACCATAC GTTTTGTGTG 
GAACACAATG CTTTTGGGAG GATTTTACAG CATGAACTCA AACCAAATGG CAGGAATATT 
CCAGTGACAG AAGAGAACAA GAAAGAATAT GTCAGGTTGT ATGTAAACTG GCGATTTATG 
AGAGGAATAG AGGCGCAGTT CCTGGCTCTG CAGAAAGGTT TTAATGAGCT TATTCCTCAG 
CATCTCCTTA AGCCTTTCGA CCAGAAGGAA CTTGAGCTAA TCATAGGAGG CCTGGATAAG 
ATAGACCTGA ACGACTGGAA GTCCAACACC CGCCTAAAGC ACTGCATGGC AGACAGCAAC 
ATCGTGAAGT GGTTCTGGCA GGCAGTGGAA ACGTTTGATG AGGAGAGAAG GGCAAGGCTG 
CTGCAGTTTG TGACAGGCTC GACGCGTGTC CCACTTCAAG GTTTCAAGGC TTTACAAGGC 
GCTGCAGGAC CCAGACTGTT TACCATCCAT TTAATAGATG CAAACACAGA CAACCTTCCA 
AAAGCCCACA CTTGCTTTAA CCGGATCGAC ATTCCGCCTT ATGAGTCATA TGAGAAGCTT 
TATGAGAAGC TGTTGACAGC TGTGGAAGAG ACGTGTGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_014936603.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014792089.1 Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCa61069
Clone ID Related Accession (Same CDS sequence)	XM_014936603.1
Accession Version	XM_014936603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090794.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGATTTCT TCAGACTTCC TGATCCCTTT 
GCAAAAATAG TTGTGGATGG GTCGGGTCAG TGCCATTCAA CTGACACTGT GAAGAACACC 
TTGGACCCCA AATGGAACCA GCATTATGAT CTATACGTTG GGAAGACAGA TTCCATAACC 
ATCAGCGTAT GGAACCATAA AAAAATCCAC AAGAAACAGG GAGCTGGCTT CCTGGGGTGT 
GTCCGACTCC TCTCCAATGC CATCAGCAGG CTAAAAGATA CTGGATACCA GCGTTTGGAT 
CTATGCAAAT TAAATCCCAC CGATACCGAT GCTGTACGAG GCCAAATAGT GGTCAGTTTA 
CAGACACGGG ACAGAATAGG TACAGGAGGT TCTGTAGTAG ACTGTAGAGG GCTTTTGGAG 
AATGAAGGAA CGGTTTATGA AGATTCCGGA CCGGGAAGGC CACTGAGCTG TTTTATGGAG 
GAACCAGCAC CGTACACGGA CACGACGGGG GCTGCTGGGG GAGGCAACTG TCGCTTCGTA 
GAGTCCCCCA GTCAAGACCA GAGGCTTCAG GCGCAGCGAC TTCGGACTCC TGAAGTCAGA 
GGACACGTCC AGACACCTCA GAACAGACCT CATGGTCACC AGTCGCCTGA CCTACCAGAA 
GGCTACGAGC AAAGAACAAC GGTACAGGGA CAGGTTTATT TTTTGCACAC ACAGACTGGA 
GTTAGCACGT GGCATGACCC TAGGATACCA AGAGACCTTA ACAGTGTGAA TTGTGATGAA 
CTGGGACCTT TGCCTCCAGG TTGGGAAGTT AGAAGTACAG TTTCAGGAAG AATATATTTT 
GTAGATCATA ACAACAGAAC CACACAATTC ACAGATCCAC GACTACATCA CATCATGAAC 
CATCAATGCC AGCTTAAAGA GCCCAGCCAG CAGCCTTTGC CAGCTCCAAA CGAGGGGTCG 
CTAGAAGACG GCGAGGAGTT GCCTGCACAA AGATATGAAA GAGACTTGGT ACAAAAGTTG 
AAAGTTCTTA GACATGAGCT CTCTCTTCAG CAACCACAGG CTGGTCACTG TCGCATTGAA 
GTATCCAGAG AAGAAATATT TGAGGAATCC TACCGTCAGA TAATGAAGAT GAGGCCAAAG 
GACTTGAAAA AGAGGCTTAT GGTGAAATTT CGCGGAGAGG AAGGCCTGGA TTATGGAGGA 
GTAGCAAGAG AATGGTTGTA TTTACTGTGT CATGAAATGT TGAATCCCTA TTATGGACTC 
TTCCAGTACT CCACAGATAA TATTTACATG CTGCAAATCA ATCCAGACTC TTCTATCAAT 
CCTGACCATT TGTCTTATTT CCATTTTGTT GGTCGGATAA TGGGTTTGGC TGTGTTCCAT 
GGACACTATA TCAACGGGGG GTTCACGGTT CCCTTTTACA AACAGCTGCT GGGGAAGCCC 
ATTCAGCTCT CCGATCTGGA ATCTGTTGAC CCAGAATTAC ACAAAAGTTT AGTCTGGATC 
TTAGAGAACG ATATCACCCC GGTTCTGGAC CATACGTTTT GTGTGGAACA CAATGCTTTT 
GGGAGGATTT TACAGCATGA ACTCAAACCA AATGGCAGGA ATATTCCAGT GACAGAAGAG 
AACAAGAAAG AATATGTCAG GTTGTATGTA AACTGGCGAT TTATGAGAGG AATAGAGGCG 
CAGTTCCTGG CTCTGCAGAA AGGTTTTAAT GAGCTTATTC CTCAGCATCT CCTTAAGCCT 
TTCGACCAGA AGGAACTTGA GCTAATCATA GGAGGCCTGG ATAAGATAGA CCTGAACGAC 
TGGAAGTCCA ACACCCGCCT AAAGCACTGC ATGGCAGACA GCAACATCGT GAAGTGGTTC 
TGGCAGGCAG TGGAAACGTT TGATGAGGAG AGAAGGGCAA GGCTGCTGCA GTTTGTGACA 
GGCTCGACGC GTGTCCCACT TCAAGGTTTC AAGGCTTTAC AAGGTTCTAC AGGCGCTGCA 
GGACCCAGAC TGTTTACCAT CCATTTAATA GATGCAAACA CAGACAACCT TCCAAAAGCC 
CACACTTGCT TTAACCGGAT CGACATTCCG CCTTATGAGT CATATGAGAA GCTTTATGAG 
AAGCTGTTGA CAGCTGTGGA AGAGACGTGT GGGTTTGCTG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014792089.1
CDS	18..2165
Translation	MVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPTDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDTTGAAGGGNCRFVESPSQDQRLQAQRLRTPEVRGHVQTPQNRPHGHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQQPLPAPNEGSLEDGEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCMADSNIVKWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_014936603.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936603.1

ORF Insert Sequence:

ATGGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGATTTCT TCAGACTTCC TGATCCCTTT 
GCAAAAATAG TTGTGGATGG GTCGGGTCAG TGCCATTCAA CTGACACTGT GAAGAACACC 
TTGGACCCCA AATGGAACCA GCATTATGAT CTATACGTTG GGAAGACAGA TTCCATAACC 
ATCAGCGTAT GGAACCATAA AAAAATCCAC AAGAAACAGG GAGCTGGCTT CCTGGGGTGT 
GTCCGACTCC TCTCCAATGC CATCAGCAGG CTAAAAGATA CTGGATACCA GCGTTTGGAT 
CTATGCAAAT TAAATCCCAC CGATACCGAT GCTGTACGAG GCCAAATAGT GGTCAGTTTA 
CAGACACGGG ACAGAATAGG TACAGGAGGT TCTGTAGTAG ACTGTAGAGG GCTTTTGGAG 
AATGAAGGAA CGGTTTATGA AGATTCCGGA CCGGGAAGGC CACTGAGCTG TTTTATGGAG 
GAACCAGCAC CGTACACGGA CACGACGGGG GCTGCTGGGG GAGGCAACTG TCGCTTCGTA 
GAGTCCCCCA GTCAAGACCA GAGGCTTCAG GCGCAGCGAC TTCGGACTCC TGAAGTCAGA 
GGACACGTCC AGACACCTCA GAACAGACCT CATGGTCACC AGTCGCCTGA CCTACCAGAA 
GGCTACGAGC AAAGAACAAC GGTACAGGGA CAGGTTTATT TTTTGCACAC ACAGACTGGA 
GTTAGCACGT GGCATGACCC TAGGATACCA AGAGACCTTA ACAGTGTGAA TTGTGATGAA 
CTGGGACCTT TGCCTCCAGG TTGGGAAGTT AGAAGTACAG TTTCAGGAAG AATATATTTT 
GTAGATCATA ACAACAGAAC CACACAATTC ACAGATCCAC GACTACATCA CATCATGAAC 
CATCAATGCC AGCTTAAAGA GCCCAGCCAG CAGCCTTTGC CAGCTCCAAA CGAGGGGTCG 
CTAGAAGACG GCGAGGAGTT GCCTGCACAA AGATATGAAA GAGACTTGGT ACAAAAGTTG 
AAAGTTCTTA GACATGAGCT CTCTCTTCAG CAACCACAGG CTGGTCACTG TCGCATTGAA 
GTATCCAGAG AAGAAATATT TGAGGAATCC TACCGTCAGA TAATGAAGAT GAGGCCAAAG 
GACTTGAAAA AGAGGCTTAT GGTGAAATTT CGCGGAGAGG AAGGCCTGGA TTATGGAGGA 
GTAGCAAGAG AATGGTTGTA TTTACTGTGT CATGAAATGT TGAATCCCTA TTATGGACTC 
TTCCAGTACT CCACAGATAA TATTTACATG CTGCAAATCA ATCCAGACTC TTCTATCAAT 
CCTGACCATT TGTCTTATTT CCATTTTGTT GGTCGGATAA TGGGTTTGGC TGTGTTCCAT 
GGACACTATA TCAACGGGGG GTTCACGGTT CCCTTTTACA AACAGCTGCT GGGGAAGCCC 
ATTCAGCTCT CCGATCTGGA ATCTGTTGAC CCAGAATTAC ACAAAAGTTT AGTCTGGATC 
TTAGAGAACG ATATCACCCC GGTTCTGGAC CATACGTTTT GTGTGGAACA CAATGCTTTT 
GGGAGGATTT TACAGCATGA ACTCAAACCA AATGGCAGGA ATATTCCAGT GACAGAAGAG 
AACAAGAAAG AATATGTCAG GTTGTATGTA AACTGGCGAT TTATGAGAGG AATAGAGGCG 
CAGTTCCTGG CTCTGCAGAA AGGTTTTAAT GAGCTTATTC CTCAGCATCT CCTTAAGCCT 
TTCGACCAGA AGGAACTTGA GCTAATCATA GGAGGCCTGG ATAAGATAGA CCTGAACGAC 
TGGAAGTCCA ACACCCGCCT AAAGCACTGC ATGGCAGACA GCAACATCGT GAAGTGGTTC 
TGGCAGGCAG TGGAAACGTT TGATGAGGAG AGAAGGGCAA GGCTGCTGCA GTTTGTGACA 
GGCTCGACGC GTGTCCCACT TCAAGGTTTC AAGGCTTTAC AAGGTTCTAC AGGCGCTGCA 
GGACCCAGAC TGTTTACCAT CCATTTAATA GATGCAAACA CAGACAACCT TCCAAAAGCC 
CACACTTGCT TTAACCGGAT CGACATTCCG CCTTATGAGT CATATGAGAA GCTTTATGAG 
AAGCTGTTGA CAGCTGTGGA AGAGACGTGT GGGTTTGCTG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_014936604.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014792090.1 Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa61070
Clone ID Related Accession (Same CDS sequence)	XM_014936604.1
Accession Version	XM_014936604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090794.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGATTTCT TCAGACTTCC TGATCCCTTT 
GCAAAAATAG TTGTGGATGG GTCGGGTCAG TGCCATTCAA CTGACACTGT GAAGAACACC 
TTGGACCCCA AATGGAACCA GCATTATGAT CTATACGTTG GGAAGACAGA TTCCATAACC 
ATCAGCGTAT GGAACCATAA AAAAATCCAC AAGAAACAGG GAGCTGGCTT CCTGGGGTGT 
GTCCGACTCC TCTCCAATGC CATCAGCAGG CTAAAAGATA CTGGATACCA GCGTTTGGAT 
CTATGCAAAT TAAATCCCAC CGATACCGAT GCTGTACGAG GCCAAATAGT GGTCAGTTTA 
CAGACACGGG ACAGAATAGG TACAGGAGGT TCTGTAGTAG ACTGTAGAGG GCTTTTGGAG 
AATGAAGGAA CGGTTTATGA AGATTCCGGA CCGGGAAGGC CACTGAGCTG TTTTATGGAG 
GAACCAGCAC CGTACACGGA CACGACGGGG GCTGCTGGGG GAGGCAACTG TCGCTTCGTA 
GAGTCCCCCA GTCAAGACCA GAGGCTTCAG GCGCAGCGAC TTCGGACTCC TGAAGTCAGA 
GGACACGTCC AGACACCTCA GAACAGACCT CATGGTCACC AGTCGCCTGA CCTACCAGAA 
GGCTACGAGC AAAGAACAAC GGTACAGGGA CAGGTTTATT TTTTGCACAC ACAGACTGGA 
GTTAGCACGT GGCATGACCC TAGGATACCA AGAGACCTTA ACAGTGTGAA TTGTGATGAA 
CTGGGACCTT TGCCTCCAGG TTGGGAAGTT AGAAGTACAG TTTCAGGAAG AATATATTTT 
GTAGATCATA ACAACAGAAC CACACAATTC ACAGATCCAC GACTACATCA CATCATGAAC 
CATCAATGCC AGCTTAAAGA GCCCAGCCAG CAGCCTTTGC CAGCTCCAAA CGAGGGGTCG 
CTAGAAGACG GCGAGGAGTT GCCTGCACAA AGATATGAAA GAGACTTGGT ACAAAAGTTG 
AAAGTTCTTA GACATGAGCT CTCTCTTCAG CAACCACAGG CTGGTCACTG TCGCATTGAA 
GTATCCAGAG AAGAAATATT TGAGGAATCC TACCGTCAGA TAATGAAGAT GAGGCCAAAG 
GACTTGAAAA AGAGGCTTAT GGTGAAATTT CGCGGAGAGG AAGGCCTGGA TTATGGAGGA 
GTAGCAAGAG AATGGTTGTA TTTACTGTGT CATGAAATGT TGAATCCCTA TTATGGACTC 
TTCCAGTACT CCACAGATAA TATTTACATG CTGCAAATCA ATCCAGACTC TTCTATCAAT 
CCTGACCATT TGTCTTATTT CCATTTTGTT GGTCGGATAA TGGGTTTGGC TGTGTTCCAT 
GGACACTATA TCAACGGGGG GTTCACGGTT CCCTTTTACA AACAGCTGCT GGGGAAGCCC 
ATTCAGCTCT CCGATCTGGA ATCTGTTGAC CCAGAATTAC ACAAAAGTTT AGTCTGGATC 
TTAGAGAACG ATATCACCCC GGTTCTGGAC CATACGTTTT GTGTGGAACA CAATGCTTTT 
GGGAGGATTT TACAGCATGA ACTCAAACCA AATGGCAGGA ATATTCCAGT GACAGAAGAG 
AACAAGAAAG AATATGTCAG GTTGTATGTA AACTGGCGAT TTATGAGAGG AATAGAGGCG 
CAGTTCCTGG CTCTGCAGAA AGGTTTTAAT GAGCTTATTC CTCAGCATCT CCTTAAGCCT 
TTCGACCAGA AGGAACTTGA GCTAATCATA GGAGGCCTGG ATAAGATAGA CCTGAACGAC 
TGGAAGTCCA ACACCCGCCT AAAGCACTGC ATGGCAGACA GCAACATCGT GAAGTGGTTC 
TGGCAGGCAG TGGAAACGTT TGATGAGGAG AGAAGGGCAA GGCTGCTGCA GTTTGTGACA 
GGCTCGACGC GTGTCCCACT TCAAGGTTTC AAGGCTTTAC AAGGCGCTGC AGGACCCAGA 
CTGTTTACCA TCCATTTAAT AGATGCAAAC ACAGACAACC TTCCAAAAGC CCACACTTGC 
TTTAACCGGA TCGACATTCC GCCTTATGAG TCATATGAGA AGCTTTATGA GAAGCTGTTG 
ACAGCTGTGG AAGAGACGTG TGGGTTTGCT GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014792090.1
CDS	18..2156
Translation	MVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPTDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDTTGAAGGGNCRFVESPSQDQRLQAQRLRTPEVRGHVQTPQNRPHGHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQQPLPAPNEGSLEDGEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCMADSNIVKWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_014936604.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936604.1

ORF Insert Sequence:

ATGGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGATTTCT TCAGACTTCC TGATCCCTTT 
GCAAAAATAG TTGTGGATGG GTCGGGTCAG TGCCATTCAA CTGACACTGT GAAGAACACC 
TTGGACCCCA AATGGAACCA GCATTATGAT CTATACGTTG GGAAGACAGA TTCCATAACC 
ATCAGCGTAT GGAACCATAA AAAAATCCAC AAGAAACAGG GAGCTGGCTT CCTGGGGTGT 
GTCCGACTCC TCTCCAATGC CATCAGCAGG CTAAAAGATA CTGGATACCA GCGTTTGGAT 
CTATGCAAAT TAAATCCCAC CGATACCGAT GCTGTACGAG GCCAAATAGT GGTCAGTTTA 
CAGACACGGG ACAGAATAGG TACAGGAGGT TCTGTAGTAG ACTGTAGAGG GCTTTTGGAG 
AATGAAGGAA CGGTTTATGA AGATTCCGGA CCGGGAAGGC CACTGAGCTG TTTTATGGAG 
GAACCAGCAC CGTACACGGA CACGACGGGG GCTGCTGGGG GAGGCAACTG TCGCTTCGTA 
GAGTCCCCCA GTCAAGACCA GAGGCTTCAG GCGCAGCGAC TTCGGACTCC TGAAGTCAGA 
GGACACGTCC AGACACCTCA GAACAGACCT CATGGTCACC AGTCGCCTGA CCTACCAGAA 
GGCTACGAGC AAAGAACAAC GGTACAGGGA CAGGTTTATT TTTTGCACAC ACAGACTGGA 
GTTAGCACGT GGCATGACCC TAGGATACCA AGAGACCTTA ACAGTGTGAA TTGTGATGAA 
CTGGGACCTT TGCCTCCAGG TTGGGAAGTT AGAAGTACAG TTTCAGGAAG AATATATTTT 
GTAGATCATA ACAACAGAAC CACACAATTC ACAGATCCAC GACTACATCA CATCATGAAC 
CATCAATGCC AGCTTAAAGA GCCCAGCCAG CAGCCTTTGC CAGCTCCAAA CGAGGGGTCG 
CTAGAAGACG GCGAGGAGTT GCCTGCACAA AGATATGAAA GAGACTTGGT ACAAAAGTTG 
AAAGTTCTTA GACATGAGCT CTCTCTTCAG CAACCACAGG CTGGTCACTG TCGCATTGAA 
GTATCCAGAG AAGAAATATT TGAGGAATCC TACCGTCAGA TAATGAAGAT GAGGCCAAAG 
GACTTGAAAA AGAGGCTTAT GGTGAAATTT CGCGGAGAGG AAGGCCTGGA TTATGGAGGA 
GTAGCAAGAG AATGGTTGTA TTTACTGTGT CATGAAATGT TGAATCCCTA TTATGGACTC 
TTCCAGTACT CCACAGATAA TATTTACATG CTGCAAATCA ATCCAGACTC TTCTATCAAT 
CCTGACCATT TGTCTTATTT CCATTTTGTT GGTCGGATAA TGGGTTTGGC TGTGTTCCAT 
GGACACTATA TCAACGGGGG GTTCACGGTT CCCTTTTACA AACAGCTGCT GGGGAAGCCC 
ATTCAGCTCT CCGATCTGGA ATCTGTTGAC CCAGAATTAC ACAAAAGTTT AGTCTGGATC 
TTAGAGAACG ATATCACCCC GGTTCTGGAC CATACGTTTT GTGTGGAACA CAATGCTTTT 
GGGAGGATTT TACAGCATGA ACTCAAACCA AATGGCAGGA ATATTCCAGT GACAGAAGAG 
AACAAGAAAG AATATGTCAG GTTGTATGTA AACTGGCGAT TTATGAGAGG AATAGAGGCG 
CAGTTCCTGG CTCTGCAGAA AGGTTTTAAT GAGCTTATTC CTCAGCATCT CCTTAAGCCT 
TTCGACCAGA AGGAACTTGA GCTAATCATA GGAGGCCTGG ATAAGATAGA CCTGAACGAC 
TGGAAGTCCA ACACCCGCCT AAAGCACTGC ATGGCAGACA GCAACATCGT GAAGTGGTTC 
TGGCAGGCAG TGGAAACGTT TGATGAGGAG AGAAGGGCAA GGCTGCTGCA GTTTGTGACA 
GGCTCGACGC GTGTCCCACT TCAAGGTTTC AAGGCTTTAC AAGGCGCTGC AGGACCCAGA 
CTGTTTACCA TCCATTTAAT AGATGCAAAC ACAGACAACC TTCCAAAAGC CCACACTTGC 
TTTAACCGGA TCGACATTCC GCCTTATGAG TCATATGAGA AGCTTTATGA GAAGCTGTTG 
ACAGCTGTGG AAGAGACGTG TGGGTTTGCT GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

SMURF1 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

SUPPORT

COMPANY

SUPPORT

COMPANY