NEMF cDNA ORF clone, Calidris pugnax(ruff)

The following NEMF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEMF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa61227 XM_014936824.1
Latest version!
Calidris pugnax nuclear export mediator factor (NEMF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa61228 XM_014936825.1
Latest version!
Calidris pugnax nuclear export mediator factor (NEMF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa61227
    Clone ID Related Accession (Same CDS sequence) XM_014936824.1
    Accession Version XM_014936824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3189bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product nuclear export mediator factor NEMF isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090795.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTATTTTC TTTTTGACAG CTTGCTGGGA ATGAGAGTAA ACAATGTTTA TGATGTGGAT 
    AATAAGACGT ATCTTATTCG CCTTCAAAAA CCAGACTGCA AGGCCACGCT GCTGCTTGAA
    TCCGGTATCC GCATTCACAC GACAGAGTTC GAGTGGCCAA AAAACATGAT GCCATCGAGC
    TTTGCAATGA AGTGCCGAAA GCATTTAAAG ACCAGAAGGC TCGTCAGTGT CAAACAGCTG
    GGCATAGACA GGATCGTGGA CTTCCAGTTC GGAAGCGACG AAGCTGCGTA TCACCTGATC
    GTTGAGCTGT ACGACAGGGG TAACATCGTC CTGACAGACT ACGAGTACGT CATTTTAAAC
    ATTCTGAGGT TTCGCACGGA TGAAGCGGAT GATGTCAGGT TCGCCGTTCG GGAGCGGTAC
    CCGGTCGACA GCGCGAAAGC GCCTGCACCT CTGCCAACCT TGGAAAGGTT AACTGAAATA
    ATATCAAATG CACCCAAAGG GGAACAACTG AAGAGGGTTC TTAACCCGCA TCTTCCTTAT
    GGAGCCACTC TCATTGAGCA TTGCCTTATA GAAGCTGGAT TTTCTGGTTA TGTCAAAATA
    GATCAGCACA TGGAAAGTAA AGAAAATATT GAAAAAGTGC TTGCTGCTTT AGAGAAAGCA
    GAAGAATATA TGACACTAAC TGACAACTTC AGTGGAAAGG GTTATATTAT CCAGAAGAGG
    GAGAAAAAGC CAAGTCTGGA ACCAGATAAG CCAGCAGAAG ATATCTACAC ATATGAGGAA
    TTTCATCCTT TCTTGTTTTC TCAACATTCA AAATGTCCGT ACTTGGAATT TGACTCATTC
    AATAAGGCGG CAGACGAGTT CTACTCCAAG CTAGAGGGAC AGAAAATCGA TCTGAAAGCA
    CTGCAGCAGG AAAAACAAGC ATTGAAGAAA CTGGAAAACG TCCGTAGGGA TCATGAGCAC
    AGACTAGAAG CTCTTCAGCA AGCTCAGGAA GCCGATAAGA TAAAAGGAGA ACTTATAGAA
    ATGAACTTGG AGATGGTGGA CCGAGCCATC CAGGTGGTGC GGAGCGCGTT GGCCAACCAG
    ATCGACTGGA CAGAGATCGG AGCCATCGTT AAAGAAGCTC AAGCGCAGGG AGACCCCGTG
    GCAAACGCAA TCAAGGAGTT AAAGCTTCAG ACAAATCATA TCACGATGCT GCTGAGGAAC
    CCATATATAT TATCAGAAGA GGAAGAGGAG GAGGAGGAGG ATGCTGATTT AGAGAAAGAA
    GAAACTGAAG AACTGAAGGG AAAAAAGAAA AAGAATAAAA GCAAACAGTT GAAGAAACCT
    CAGAAAAACA AACCGTCGTT AGTTGATGTT GATCTCAGTT TGTCTGCATA CGCCAATGCC
    AAAAAGTACT ACGATCACAA AAGACATGCA GCCAAGAAGA CTCAGAAGAC GGTAGAAGCT
    GCAGAAAAGG CGTTTAAATC GGCTGAGAAG AAGACGAAGC AGACGTTGAG GGAGGTTCAG
    ACAGTGACCA CCATTCAGAA GGCGAGGAAG GTCTATTGGT TTGAGAAGTT CCTGTGGTTC
    ATTAGTTCCG AGAATTACCT GATTATTGCT GGGAGAGATC AACAGCAGAA CGAGCTGATT
    GTAAAGCGGT ATCTTAAACC AGGGGACATA TATGTGCACG CCGATCTCCA TGGAGCCACC
    AGCTGTGTCA TCAAGAACCC ATCAGGTGAA CCCATCCCTC CACGAACCCT GACAGAGGCA
    GGCACCATGG CCTTGTGTTA CAGCGCTGCC TGGGATGCCC GGGTTGTCAC CAGTGCTTGG
    TGGGTCTCCC ACAACCAGGT TTCTAAGACT GCACCTACAG GAGAATACCT CACTACTGGG
    AGCTTCATGA TCCGAGGGAA GAAGAATTTT CTTCCACCTT CGTATCTGAT GATGGGCTTC
    AGCTTCTTGT TTAAGGTGGA TGAAAGCTGC GTCTGGAGAC ACCGAGAAGA AAGGAAGATC
    AAAGTGCAGG ATGAGGATCT GGAAACGGTT TCCAGCAGTG CCAGTGAACT GGTGGCTGAA
    GAAGTGGAGC TGTTAGAAGG AGGAGACAGC AGCAGCGAAG AAGAAAAAGT GGAGTGTCAG
    GAAGCACCAG AGGACATGGA AGCCACATCT GAGAGTCATC ATGACGAGGG CGTTGCTGAC
    CTTGACCAGG GCAGAGGAAA CACATCTCCT GCACCAGAGG GTGACTCCGA AGAGGAGGAT
    GGAGAATCTG AAGATGAAGC AGACCATCCA GAGTCAAAGA GCGAGGTGAA GGAGGAAGAG
    GTAAACTACC CAGACACAAC AATCGACCTG TCACATCTTC AGTCTCAGAG ATCCCTGCAG
    AAATTCACCC CCAAAGAGGA AGAAGAACCC AACCTGAGTG ACAGCAAGTC CCAGGGGCGA
    AGGCATCTGT CTGCCAAGGA GCGGAGAGAA ATGAAGAAAA AGAAGCAGCA AAACGACTCT
    GAGAACCTGG AGCTGCCCGA AGAGCAGCAG AGAAAGTCGG AGACGCAGCC TCCCCCTGCT
    CCCAACACCA CTAAGGGGGT GCCAGCCCCC CAGCCCATCA AGCGGGGCCA GAAGAGTAAA
    ATGAAGAAGA TGAAGGAAAA GTACAAGGAC CAGGATGAGG AGGACCGGGA GCTCATCATG
    AAGCTGCTGG GGTCTGCAGG GTCCAACAAG GAGGAGAAAG GGAAAAAAGG CAAAAAGGGG
    AAGACGAAAG AAGAGCCGGC GAAGAAGCAG CAGCAGAAAC CCAAGGCTGC GCGTCGCGGC
    GCTGGAGGGG CCAAGGAGCC CTTCCCAGCA GGAATCCTGC TGCACGAGTC GCAGGACCCA
    GCCCTGGAGG AGCAGCAGGA CGAGAAGGAG GAGCAAGACC AGGACCAGCC GGCGGTGGAG
    GAAGGCGAGG CGCTGCTGGA CTCCCTGACG GGCCAACCCC ACCCCGAGGA CATCCTCCTC
    TTCGCCGTCC CCATCTGCGC TCCCTACACG GCCATGGCCA ACTACAAGTA CAAAGTCAAG
    CTCACGCCAG GCACCCAGAA GAAGGGAAAA GCTGCAAAAA TTGCCTTGCA TAATTTTATG
    CAATCCAAAG AAGCTACTGT GAGAGAAAAA GACCTGTTCC GCAGCGTGAA GGACACCGAT
    TTGTCAAGAA ATATTCCCGG GAAAGTGAAA GTGTCTGCGC CTCATCTCCT CAACAGGAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014792310.1
    CDS16..3204
    Translation

    Target ORF information:

    RefSeq Version XM_014936824.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax nuclear export mediator factor (NEMF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014936824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTATTTTC TTTTTGACAG CTTGCTGGGA ATGAGAGTAA ACAATGTTTA TGATGTGGAT 
    AATAAGACGT ATCTTATTCG CCTTCAAAAA CCAGACTGCA AGGCCACGCT GCTGCTTGAA
    TCCGGTATCC GCATTCACAC GACAGAGTTC GAGTGGCCAA AAAACATGAT GCCATCGAGC
    TTTGCAATGA AGTGCCGAAA GCATTTAAAG ACCAGAAGGC TCGTCAGTGT CAAACAGCTG
    GGCATAGACA GGATCGTGGA CTTCCAGTTC GGAAGCGACG AAGCTGCGTA TCACCTGATC
    GTTGAGCTGT ACGACAGGGG TAACATCGTC CTGACAGACT ACGAGTACGT CATTTTAAAC
    ATTCTGAGGT TTCGCACGGA TGAAGCGGAT GATGTCAGGT TCGCCGTTCG GGAGCGGTAC
    CCGGTCGACA GCGCGAAAGC GCCTGCACCT CTGCCAACCT TGGAAAGGTT AACTGAAATA
    ATATCAAATG CACCCAAAGG GGAACAACTG AAGAGGGTTC TTAACCCGCA TCTTCCTTAT
    GGAGCCACTC TCATTGAGCA TTGCCTTATA GAAGCTGGAT TTTCTGGTTA TGTCAAAATA
    GATCAGCACA TGGAAAGTAA AGAAAATATT GAAAAAGTGC TTGCTGCTTT AGAGAAAGCA
    GAAGAATATA TGACACTAAC TGACAACTTC AGTGGAAAGG GTTATATTAT CCAGAAGAGG
    GAGAAAAAGC CAAGTCTGGA ACCAGATAAG CCAGCAGAAG ATATCTACAC ATATGAGGAA
    TTTCATCCTT TCTTGTTTTC TCAACATTCA AAATGTCCGT ACTTGGAATT TGACTCATTC
    AATAAGGCGG CAGACGAGTT CTACTCCAAG CTAGAGGGAC AGAAAATCGA TCTGAAAGCA
    CTGCAGCAGG AAAAACAAGC ATTGAAGAAA CTGGAAAACG TCCGTAGGGA TCATGAGCAC
    AGACTAGAAG CTCTTCAGCA AGCTCAGGAA GCCGATAAGA TAAAAGGAGA ACTTATAGAA
    ATGAACTTGG AGATGGTGGA CCGAGCCATC CAGGTGGTGC GGAGCGCGTT GGCCAACCAG
    ATCGACTGGA CAGAGATCGG AGCCATCGTT AAAGAAGCTC AAGCGCAGGG AGACCCCGTG
    GCAAACGCAA TCAAGGAGTT AAAGCTTCAG ACAAATCATA TCACGATGCT GCTGAGGAAC
    CCATATATAT TATCAGAAGA GGAAGAGGAG GAGGAGGAGG ATGCTGATTT AGAGAAAGAA
    GAAACTGAAG AACTGAAGGG AAAAAAGAAA AAGAATAAAA GCAAACAGTT GAAGAAACCT
    CAGAAAAACA AACCGTCGTT AGTTGATGTT GATCTCAGTT TGTCTGCATA CGCCAATGCC
    AAAAAGTACT ACGATCACAA AAGACATGCA GCCAAGAAGA CTCAGAAGAC GGTAGAAGCT
    GCAGAAAAGG CGTTTAAATC GGCTGAGAAG AAGACGAAGC AGACGTTGAG GGAGGTTCAG
    ACAGTGACCA CCATTCAGAA GGCGAGGAAG GTCTATTGGT TTGAGAAGTT CCTGTGGTTC
    ATTAGTTCCG AGAATTACCT GATTATTGCT GGGAGAGATC AACAGCAGAA CGAGCTGATT
    GTAAAGCGGT ATCTTAAACC AGGGGACATA TATGTGCACG CCGATCTCCA TGGAGCCACC
    AGCTGTGTCA TCAAGAACCC ATCAGGTGAA CCCATCCCTC CACGAACCCT GACAGAGGCA
    GGCACCATGG CCTTGTGTTA CAGCGCTGCC TGGGATGCCC GGGTTGTCAC CAGTGCTTGG
    TGGGTCTCCC ACAACCAGGT TTCTAAGACT GCACCTACAG GAGAATACCT CACTACTGGG
    AGCTTCATGA TCCGAGGGAA GAAGAATTTT CTTCCACCTT CGTATCTGAT GATGGGCTTC
    AGCTTCTTGT TTAAGGTGGA TGAAAGCTGC GTCTGGAGAC ACCGAGAAGA AAGGAAGATC
    AAAGTGCAGG ATGAGGATCT GGAAACGGTT TCCAGCAGTG CCAGTGAACT GGTGGCTGAA
    GAAGTGGAGC TGTTAGAAGG AGGAGACAGC AGCAGCGAAG AAGAAAAAGT GGAGTGTCAG
    GAAGCACCAG AGGACATGGA AGCCACATCT GAGAGTCATC ATGACGAGGG CGTTGCTGAC
    CTTGACCAGG GCAGAGGAAA CACATCTCCT GCACCAGAGG GTGACTCCGA AGAGGAGGAT
    GGAGAATCTG AAGATGAAGC AGACCATCCA GAGTCAAAGA GCGAGGTGAA GGAGGAAGAG
    GTAAACTACC CAGACACAAC AATCGACCTG TCACATCTTC AGTCTCAGAG ATCCCTGCAG
    AAATTCACCC CCAAAGAGGA AGAAGAACCC AACCTGAGTG ACAGCAAGTC CCAGGGGCGA
    AGGCATCTGT CTGCCAAGGA GCGGAGAGAA ATGAAGAAAA AGAAGCAGCA AAACGACTCT
    GAGAACCTGG AGCTGCCCGA AGAGCAGCAG AGAAAGTCGG AGACGCAGCC TCCCCCTGCT
    CCCAACACCA CTAAGGGGGT GCCAGCCCCC CAGCCCATCA AGCGGGGCCA GAAGAGTAAA
    ATGAAGAAGA TGAAGGAAAA GTACAAGGAC CAGGATGAGG AGGACCGGGA GCTCATCATG
    AAGCTGCTGG GGTCTGCAGG GTCCAACAAG GAGGAGAAAG GGAAAAAAGG CAAAAAGGGG
    AAGACGAAAG AAGAGCCGGC GAAGAAGCAG CAGCAGAAAC CCAAGGCTGC GCGTCGCGGC
    GCTGGAGGGG CCAAGGAGCC CTTCCCAGCA GGAATCCTGC TGCACGAGTC GCAGGACCCA
    GCCCTGGAGG AGCAGCAGGA CGAGAAGGAG GAGCAAGACC AGGACCAGCC GGCGGTGGAG
    GAAGGCGAGG CGCTGCTGGA CTCCCTGACG GGCCAACCCC ACCCCGAGGA CATCCTCCTC
    TTCGCCGTCC CCATCTGCGC TCCCTACACG GCCATGGCCA ACTACAAGTA CAAAGTCAAG
    CTCACGCCAG GCACCCAGAA GAAGGGAAAA GCTGCAAAAA TTGCCTTGCA TAATTTTATG
    CAATCCAAAG AAGCTACTGT GAGAGAAAAA GACCTGTTCC GCAGCGTGAA GGACACCGAT
    TTGTCAAGAA ATATTCCCGG GAAAGTGAAA GTGTCTGCGC CTCATCTCCT CAACAGGAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa61228
    Clone ID Related Accession (Same CDS sequence) XM_014936825.1
    Accession Version XM_014936825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product nuclear export mediator factor NEMF isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090795.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTATTTTC TTTTTGACAG CTTGCTGGGA ATGAGAGTAA ACAATGTTTA TGATGTGGAT 
    AATAAGACGT ATCTTATTCG CCTTCAAAAA CCAGACTGCA AGGCCACGCT GCTGCTTGAA
    TCCGGTATCC GCATTCACAC GACAGAGTTC GAGTGGCCAA AAAACATGAT GCCATCGAGC
    TTTGCAATGA AGTGCCGAAA GCATTTAAAG ACCAGAAGGC TCGTCAGTGT CAAACAGCTG
    GGCATAGACA GGATCGTGGA CTTCCAGTTC GGAAGCGACG AAGCTGCGTA TCACCTGATC
    GTTGAGCTGT ACGACAGGGG TAACATCGTC CTGACAGACT ACGAGTACGT CATTTTAAAC
    ATTCTGAGGT TTCGCACGGA TGAAGCGGAT GATGTCAGGT TCGCCGTTCG GGAGCGGTAC
    CCGGTCGACA GCGCGAAAGC GCCTGCACCT CTGCCAACCT TGGAAAGGTT AACTGAAATA
    ATATCAAATG CACCCAAAGG GGAACAACTG AAGAGGGTTC TTAACCCGCA TCTTCCTTAT
    GGAGCCACTC TCATTGAGCA TTGCCTTATA GAAGCTGGAT TTTCTGGTTA TGTCAAAATA
    GATCAGCACA TGGAAAGTAA AGAAAATATT GAAAAAGTGC TTGCTGCTTT AGAGAAAGCA
    GAAGAATATA TGACACTAAC TGACAACTTC AGTGGAAAGG GTTATATTAT CCAGAAGAGG
    GAGAAAAAGC CAAGTCTGGA ACCAGATAAG CCAGCAGAAG ATATCTACAC ATATGAGGAA
    TTTCATCCTT TCTTGTTTTC TCAACATTCA AAATGTCCGT ACTTGGAATT TGACTCATTC
    AATAAGGCGG CAGACGAGTT CTACTCCAAG CTAGAGGGAC AGAAAATCGA TCTGAAAGCA
    CTGCAGCAGG AAAAACAAGC ATTGAAGAAA CTGGAAAACG TCCGTAGGGA TCATGAGCAC
    AGACTAGAAG CTCTTCAGCA AGCTCAGGAA GCCGATAAGA TAAAAGGAGA ACTTATAGAA
    ATGAACTTGG AGATGGTGGA CCGAGCCATC CAGGTGGTGC GGAGCGCGTT GGCCAACCAG
    ATCGACTGGA CAGAGATCGG AGCCATCGTT AAAGAAGCTC AAGCGCAGGG AGACCCCGTG
    GCAAACGCAA TCAAGGAGTT AAAGCTTCAG ACAAATCATA TCACGATGCT GCTGAGGAAC
    CCATATATAT TATCAGAAGA GGAAGAGGAG GAGGAGGAGG ATGCTGATTT AGAGAAAGAA
    GAAACTGAAG AACTGAAGGG AAAAAAGAAA AAGAATAAAA GCAAACAGTT GAAGAAACCT
    CAGAAAAACA AACCGTCGTT AGTTGATGTT GATCTCAGTT TGTCTGCATA CGCCAATGCC
    AAAAAGTACT ACGATCACAA AAGACATGCA GCCAAGAAGA CTCAGAAGAC GGTAGAAGCT
    GCAGAAAAGG CGTTTAAATC GGCTGAGAAG AAGACGAAGC AGACGTTGAG GGAGGTTCAG
    ACAGTGACCA CCATTCAGAA GGCGAGGAAG GTCTATTGGT TTGAGAAGTT CCTGTGGTTC
    ATTAGTTCCG AGAATTACCT GATTATTGCT GGGAGAGATC AACAGCAGAA CGAGCTGATT
    GTAAAGCGGT ATCTTAAACC AGGGGACATA TATGTGCACG CCGATCTCCA TGGAGCCACC
    AGCTGTGTCA TCAAGAACCC ATCAGGTGAA CCCATCCCTC CACGAACCCT GACAGAGGCA
    GGCACCATGG CCTTGTGTTA CAGCGCTGCC TGGGATGCCC GGGTTGTCAC CAGTGCTTGG
    TGGGTCTCCC ACAACCAGGT TTCTAAGACT GCACCTACAG GAGAATACCT CACTACTGGG
    AGCTTCATGA TCCGAGGGAA GAAGAATTTT CTTCCACCTT CGTATCTGAT GATGGGCTTC
    AGCTTCTTGT TTAAGGTGGA TGAAAGCTGC GTCTGGAGAC ACCGAGAAGA AAGGAAGATC
    AAAGTGCAGG ATGAGGATCT GGAAACGGTT TCCAGCAGTG CCAGTGAACT GGTGGCTGAA
    GAAGTGGAGC TGTTAGGAGG AGACAGCAGC AGCGAAGAAG AAAAAGTGGA GTGTCAGGAA
    GCACCAGAGG ACATGGAAGC CACATCTGAG AGTCATCATG ACGAGGGCGT TGCTGACCTT
    GACCAGGGCA GAGGAAACAC ATCTCCTGCA CCAGAGGGTG ACTCCGAAGA GGAGGATGGA
    GAATCTGAAG ATGAAGCAGA CCATCCAGAG TCAAAGAGCG AGGTGAAGGA GGAAGAGGTA
    AACTACCCAG ACACAACAAT CGACCTGTCA CATCTTCAGT CTCAGAGATC CCTGCAGAAA
    TTCACCCCCA AAGAGGAAGA AGAACCCAAC CTGAGTGACA GCAAGTCCCA GGGGCGAAGG
    CATCTGTCTG CCAAGGAGCG GAGAGAAATG AAGAAAAAGA AGCAGCAAAA CGACTCTGAG
    AACCTGGAGC TGCCCGAAGA GCAGCAGAGA AAGTCGGAGA CGCAGCCTCC CCCTGCTCCC
    AACACCACTA AGGGGGTGCC AGCCCCCCAG CCCATCAAGC GGGGCCAGAA GAGTAAAATG
    AAGAAGATGA AGGAAAAGTA CAAGGACCAG GATGAGGAGG ACCGGGAGCT CATCATGAAG
    CTGCTGGGGT CTGCAGGGTC CAACAAGGAG GAGAAAGGGA AAAAAGGCAA AAAGGGGAAG
    ACGAAAGAAG AGCCGGCGAA GAAGCAGCAG CAGAAACCCA AGGCTGCGCG TCGCGGCGCT
    GGAGGGGCCA AGGAGCCCTT CCCAGCAGGA ATCCTGCTGC ACGAGTCGCA GGACCCAGCC
    CTGGAGGAGC AGCAGGACGA GAAGGAGGAG CAAGACCAGG ACCAGCCGGC GGTGGAGGAA
    GGCGAGGCGC TGCTGGACTC CCTGACGGGC CAACCCCACC CCGAGGACAT CCTCCTCTTC
    GCCGTCCCCA TCTGCGCTCC CTACACGGCC ATGGCCAACT ACAAGTACAA AGTCAAGCTC
    ACGCCAGGCA CCCAGAAGAA GGGAAAAGCT GCAAAAATTG CCTTGCATAA TTTTATGCAA
    TCCAAAGAAG CTACTGTGAG AGAAAAAGAC CTGTTCCGCA GCGTGAAGGA CACCGATTTG
    TCAAGAAATA TTCCCGGGAA AGTGAAAGTG TCTGCGCCTC ATCTCCTCAA CAGGAAAAAG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014792311.1
    CDS16..3201
    Translation

    Target ORF information:

    RefSeq Version XM_014936825.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax nuclear export mediator factor (NEMF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014936825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTATTTTC TTTTTGACAG CTTGCTGGGA ATGAGAGTAA ACAATGTTTA TGATGTGGAT 
    AATAAGACGT ATCTTATTCG CCTTCAAAAA CCAGACTGCA AGGCCACGCT GCTGCTTGAA
    TCCGGTATCC GCATTCACAC GACAGAGTTC GAGTGGCCAA AAAACATGAT GCCATCGAGC
    TTTGCAATGA AGTGCCGAAA GCATTTAAAG ACCAGAAGGC TCGTCAGTGT CAAACAGCTG
    GGCATAGACA GGATCGTGGA CTTCCAGTTC GGAAGCGACG AAGCTGCGTA TCACCTGATC
    GTTGAGCTGT ACGACAGGGG TAACATCGTC CTGACAGACT ACGAGTACGT CATTTTAAAC
    ATTCTGAGGT TTCGCACGGA TGAAGCGGAT GATGTCAGGT TCGCCGTTCG GGAGCGGTAC
    CCGGTCGACA GCGCGAAAGC GCCTGCACCT CTGCCAACCT TGGAAAGGTT AACTGAAATA
    ATATCAAATG CACCCAAAGG GGAACAACTG AAGAGGGTTC TTAACCCGCA TCTTCCTTAT
    GGAGCCACTC TCATTGAGCA TTGCCTTATA GAAGCTGGAT TTTCTGGTTA TGTCAAAATA
    GATCAGCACA TGGAAAGTAA AGAAAATATT GAAAAAGTGC TTGCTGCTTT AGAGAAAGCA
    GAAGAATATA TGACACTAAC TGACAACTTC AGTGGAAAGG GTTATATTAT CCAGAAGAGG
    GAGAAAAAGC CAAGTCTGGA ACCAGATAAG CCAGCAGAAG ATATCTACAC ATATGAGGAA
    TTTCATCCTT TCTTGTTTTC TCAACATTCA AAATGTCCGT ACTTGGAATT TGACTCATTC
    AATAAGGCGG CAGACGAGTT CTACTCCAAG CTAGAGGGAC AGAAAATCGA TCTGAAAGCA
    CTGCAGCAGG AAAAACAAGC ATTGAAGAAA CTGGAAAACG TCCGTAGGGA TCATGAGCAC
    AGACTAGAAG CTCTTCAGCA AGCTCAGGAA GCCGATAAGA TAAAAGGAGA ACTTATAGAA
    ATGAACTTGG AGATGGTGGA CCGAGCCATC CAGGTGGTGC GGAGCGCGTT GGCCAACCAG
    ATCGACTGGA CAGAGATCGG AGCCATCGTT AAAGAAGCTC AAGCGCAGGG AGACCCCGTG
    GCAAACGCAA TCAAGGAGTT AAAGCTTCAG ACAAATCATA TCACGATGCT GCTGAGGAAC
    CCATATATAT TATCAGAAGA GGAAGAGGAG GAGGAGGAGG ATGCTGATTT AGAGAAAGAA
    GAAACTGAAG AACTGAAGGG AAAAAAGAAA AAGAATAAAA GCAAACAGTT GAAGAAACCT
    CAGAAAAACA AACCGTCGTT AGTTGATGTT GATCTCAGTT TGTCTGCATA CGCCAATGCC
    AAAAAGTACT ACGATCACAA AAGACATGCA GCCAAGAAGA CTCAGAAGAC GGTAGAAGCT
    GCAGAAAAGG CGTTTAAATC GGCTGAGAAG AAGACGAAGC AGACGTTGAG GGAGGTTCAG
    ACAGTGACCA CCATTCAGAA GGCGAGGAAG GTCTATTGGT TTGAGAAGTT CCTGTGGTTC
    ATTAGTTCCG AGAATTACCT GATTATTGCT GGGAGAGATC AACAGCAGAA CGAGCTGATT
    GTAAAGCGGT ATCTTAAACC AGGGGACATA TATGTGCACG CCGATCTCCA TGGAGCCACC
    AGCTGTGTCA TCAAGAACCC ATCAGGTGAA CCCATCCCTC CACGAACCCT GACAGAGGCA
    GGCACCATGG CCTTGTGTTA CAGCGCTGCC TGGGATGCCC GGGTTGTCAC CAGTGCTTGG
    TGGGTCTCCC ACAACCAGGT TTCTAAGACT GCACCTACAG GAGAATACCT CACTACTGGG
    AGCTTCATGA TCCGAGGGAA GAAGAATTTT CTTCCACCTT CGTATCTGAT GATGGGCTTC
    AGCTTCTTGT TTAAGGTGGA TGAAAGCTGC GTCTGGAGAC ACCGAGAAGA AAGGAAGATC
    AAAGTGCAGG ATGAGGATCT GGAAACGGTT TCCAGCAGTG CCAGTGAACT GGTGGCTGAA
    GAAGTGGAGC TGTTAGGAGG AGACAGCAGC AGCGAAGAAG AAAAAGTGGA GTGTCAGGAA
    GCACCAGAGG ACATGGAAGC CACATCTGAG AGTCATCATG ACGAGGGCGT TGCTGACCTT
    GACCAGGGCA GAGGAAACAC ATCTCCTGCA CCAGAGGGTG ACTCCGAAGA GGAGGATGGA
    GAATCTGAAG ATGAAGCAGA CCATCCAGAG TCAAAGAGCG AGGTGAAGGA GGAAGAGGTA
    AACTACCCAG ACACAACAAT CGACCTGTCA CATCTTCAGT CTCAGAGATC CCTGCAGAAA
    TTCACCCCCA AAGAGGAAGA AGAACCCAAC CTGAGTGACA GCAAGTCCCA GGGGCGAAGG
    CATCTGTCTG CCAAGGAGCG GAGAGAAATG AAGAAAAAGA AGCAGCAAAA CGACTCTGAG
    AACCTGGAGC TGCCCGAAGA GCAGCAGAGA AAGTCGGAGA CGCAGCCTCC CCCTGCTCCC
    AACACCACTA AGGGGGTGCC AGCCCCCCAG CCCATCAAGC GGGGCCAGAA GAGTAAAATG
    AAGAAGATGA AGGAAAAGTA CAAGGACCAG GATGAGGAGG ACCGGGAGCT CATCATGAAG
    CTGCTGGGGT CTGCAGGGTC CAACAAGGAG GAGAAAGGGA AAAAAGGCAA AAAGGGGAAG
    ACGAAAGAAG AGCCGGCGAA GAAGCAGCAG CAGAAACCCA AGGCTGCGCG TCGCGGCGCT
    GGAGGGGCCA AGGAGCCCTT CCCAGCAGGA ATCCTGCTGC ACGAGTCGCA GGACCCAGCC
    CTGGAGGAGC AGCAGGACGA GAAGGAGGAG CAAGACCAGG ACCAGCCGGC GGTGGAGGAA
    GGCGAGGCGC TGCTGGACTC CCTGACGGGC CAACCCCACC CCGAGGACAT CCTCCTCTTC
    GCCGTCCCCA TCTGCGCTCC CTACACGGCC ATGGCCAACT ACAAGTACAA AGTCAAGCTC
    ACGCCAGGCA CCCAGAAGAA GGGAAAAGCT GCAAAAATTG CCTTGCATAA TTTTATGCAA
    TCCAAAGAAG CTACTGTGAG AGAAAAAGAC CTGTTCCGCA GCGTGAAGGA CACCGATTTG
    TCAAGAAATA TTCCCGGGAA AGTGAAAGTG TCTGCGCCTC ATCTCCTCAA CAGGAAAAAG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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