EPB41L3 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPB41L3
Entrez Gene ID	106885982
Full Name	erythrocyte membrane protein band 4.1 like 3
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014938527.1	XP_014794013.1	band 4.1-like protein 3 isoform X1
XM_014938528.1	XP_014794014.1	band 4.1-like protein 3 isoform X2
XM_014938531.1	XP_014794017.1	band 4.1-like protein 3 isoform X4
XM_014938533.1	XP_014794019.1	band 4.1-like protein 3 isoform X6
XM_014938529.1	XP_014794015.1	band 4.1-like protein 3 isoform X3
XM_014938532.1	XP_014794018.1	band 4.1-like protein 3 isoform X5
XM_014938534.1	XP_014794020.1	band 4.1-like protein 3 isoform X7

Pathways from BioSystems
Gene Ontology

The following EPB41L3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPB41L3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa62523	XM_014938534.1 Latest version!	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa62517	XM_014938527.1 Latest version!	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa62518	XM_014938528.1 Latest version!	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa62519	XM_014938529.1 Latest version!	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa62520	XM_014938531.1 Latest version!	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa62521	XM_014938532.1 Latest version!	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa62522	XM_014938533.1 Latest version!	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPB41L3 ( XM_014938534.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794020.1 Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCa62523
Clone ID Related Accession (Same CDS sequence)	XM_014938534.1
Accession Version	XM_014938534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	band 4.1-like protein 3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGACTAA GCAGTCCACC GGTGAAAAAA CCTTGGATGG TTCAGACATC 
TTCAGTTTAA TAGAGTCCGC CAGAAAACCG ACTGAGTTCA TAGGAGGGGT TACTTCTACT 
TCACATAGCT GGGCTCAGAG AATAGACACG ACAACATCTG AGGAGGTAAC TTCCAGTGAA 
ACGAAGCAGG CCGTTCAGCC GCATCAGGAC ACGGTGAAGA AGGTTGTCCA GGAGACCGTG 
ATTGTGGAGG AGCGACACGG GATGAACGTG CAGGCGAGCG GGGACCCTGC TAAAGCGGCT 
GGCCTTGCGC TGGATGCCCA GGCGGAGGCT GCTGGTGCCA AGGGCAAGGA GGGCTCTGCT 
GGGACTGAGG GGGAGAAGGA GGGAAAAAGG GAAGAGGCTG GGAAAGCCCC CACCAAACTG 
GAAGGAGTCG CTGCTGCTTC GAGAGAGCAG GTGGAGGAGC ACGCTACAAC AGGCCACCTT 
GCAGAGTCTT TGGAAAGAAA ATCTCACTTT GAGTCGCCAG TTGTGAAAAC TGAGACTATC 
AGTTTCAGTA GCGTTTCTGC TGGTGGGGAA AACCTTGAAA TTGCAACAAA GGAAGTGCCA 
GTAGTCCATA CAGAAACAAA AACCATTACA TATGAGTCTT CTCAGGTGGA TTGTGGTGCT 
GACTCAGAAC CGGGTGTATT GATGAGTGCA CAGACTATCA CATCTGAAAC CACCAGCACT 
ACAACCACTA CACATATCAC CAAAACTGTG AAAGGAGGCA TCTCAGAGAC AAGAATCGAA 
AAACGAATAG TCATCACAGG AGATGCAGAT ATTGATCACG ATCAGGCGCT GGCTCAGGCA 
ATTAAAGAAG CCAAAGAGCA GCACCCTGAC ATGTCAGTGA CCAAAGTAGT GGTACATAAA 
GAGACAGAAA TCACACCAGA AGATGGGGAG GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794020.1
CDS	150..2645
Translation	MTTESGSDSESKDKEQDQQESPAQQGAAETQPQEQQEQQQQSEEHQNALEQFSAVAAHSTPVKKEQATDKEQEFAAASAKQLEYQQLEDDKLSQKSSSSKLSKSPLKIVKKPKNMQCKVTLLDGSEYACEVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDTENQKNWLDPAKEIKKQIRSGAWQFAFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDYDPDEYGSDYVSEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRASALIDRPAPYFERSSSKRYTMSRSLDGASVNENHEMYMKDSVSAAEVGTGQYATTKGISQTNLITTVTPEKKAEEEKDDEEGKKKRAEEVTPVSAIRHDTKTKQSTGEKTLDGSDIFSLIESARKPTEFIGGVTSTSHSWAQRIDTTTSEEVTSSETKQAVQPHQDTVKKVVQETVIVEERHGMNVQASGDPAKAAGLALDAQAEAAGAKGKEGSAGTEGEKEGKREEAGKAPTKLEGVAAASREQVEEHATTGHLAESLERKSHFESPVVKTETISFSSVSAGGENLEIATKEVPVVHTETKTITYESSQVDCGADSEPGVLMSAQTITSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEITPEDGED

Target ORF information:

RefSeq Version	XM_014938534.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014938534.1

ORF Insert Sequence:

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGACTAA GCAGTCCACC GGTGAAAAAA CCTTGGATGG TTCAGACATC 
TTCAGTTTAA TAGAGTCCGC CAGAAAACCG ACTGAGTTCA TAGGAGGGGT TACTTCTACT 
TCACATAGCT GGGCTCAGAG AATAGACACG ACAACATCTG AGGAGGTAAC TTCCAGTGAA 
ACGAAGCAGG CCGTTCAGCC GCATCAGGAC ACGGTGAAGA AGGTTGTCCA GGAGACCGTG 
ATTGTGGAGG AGCGACACGG GATGAACGTG CAGGCGAGCG GGGACCCTGC TAAAGCGGCT 
GGCCTTGCGC TGGATGCCCA GGCGGAGGCT GCTGGTGCCA AGGGCAAGGA GGGCTCTGCT 
GGGACTGAGG GGGAGAAGGA GGGAAAAAGG GAAGAGGCTG GGAAAGCCCC CACCAAACTG 
GAAGGAGTCG CTGCTGCTTC GAGAGAGCAG GTGGAGGAGC ACGCTACAAC AGGCCACCTT 
GCAGAGTCTT TGGAAAGAAA ATCTCACTTT GAGTCGCCAG TTGTGAAAAC TGAGACTATC 
AGTTTCAGTA GCGTTTCTGC TGGTGGGGAA AACCTTGAAA TTGCAACAAA GGAAGTGCCA 
GTAGTCCATA CAGAAACAAA AACCATTACA TATGAGTCTT CTCAGGTGGA TTGTGGTGCT 
GACTCAGAAC CGGGTGTATT GATGAGTGCA CAGACTATCA CATCTGAAAC CACCAGCACT 
ACAACCACTA CACATATCAC CAAAACTGTG AAAGGAGGCA TCTCAGAGAC AAGAATCGAA 
AAACGAATAG TCATCACAGG AGATGCAGAT ATTGATCACG ATCAGGCGCT GGCTCAGGCA 
ATTAAAGAAG CCAAAGAGCA GCACCCTGAC ATGTCAGTGA CCAAAGTAGT GGTACATAAA 
GAGACAGAAA TCACACCAGA AGATGGGGAG GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPB41L3 ( XM_014938527.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794013.1 Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa62517
Clone ID Related Accession (Same CDS sequence)	XM_014938527.1
Accession Version	XM_014938527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	band 4.1-like protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGCCATC TTTGCTTGGC CCCGACTCCC GCCACCGCCA CCCCCCGCCA 
TCCTCGCCGC CTGGCCCCCA CGCACCTCCC GCCAAGCTCC GCAGGAGATG CAAGGAGAGC 
GCTGGAGCAA AGCCCGCGGG GGGCGAGGGG CCCAAGGAGA GCGCTCCCCG GCACGGCGAG 
CCGGAGCCCG ACTCCGACAG AAAGGGGCGA GTTTGCTCCC CCGAGCAAGA CGTGCCTTTC 
GCCTATAAGC AAAAAGCCGG CAAAGGCGGA ACGCTGTTTT CCTTCTCCTT GCAACTCCCG 
GAGTCCTTTC CTTCCCTCCT GGATGACGAC GGCTACCTGT CGCTCCCTAA CCTCTCCGAA 
ACCAACCTCC TGCCTGCCAG CGTGCAGCAC TACCTTCCCA TCCGCTCCCC CTCCCTGGTG 
CCTTGCTTCC TCTTCATTTT TTTCTTTCTG CTCTCTGCCT CTTTCTCAGT GCCATACGCC 
CTCACTCTCT CCTTCCCTCT GGCTATGTGC CTCTGCTACC TGGAGCCCAA GGCGGCCTCC 
TTGAGTGCCT CACTTGCCAA TGACCTGAGT GACAGTTCAG AGGAAGAGAC TGACAGTGAG 
CAGACGGACA CTGCAGCTGA TGGTGAGACC ACTGCCACCG AGTCGGATCA AGACGAGGAC 
GGAGATCTAA AGGCACAGAA TAGTCTGATT AAGCGAATAC AGGGTGAAAA TGTGTATGTC 
AAACATAGTA ATCTTATGTT AGAGGACCTA GACAAAACCC AGGAAGATCT GATGAAACAT 
CAGACCAATA TAAGCGAGCT GAAACGCACC TTCTTGGAAA CCTCCACAGA AACAACTGTG 
TCTAATGAGT GGGAGAAGAG GCTTTCCACA TCTCCCGTTC GGCTTGCAGC AAGGCAGGAG 
GAAGCTCCTA TGATTGAACC ACTAGTGCCT GAAGAGACCA AAGAAGAAAG AGAAAAATCA 
GAAAAGCTCA TATTTTTGCA GAAGGGAGGT ACTACATTCC TTGAATTGCA GCCAAGTGTG 
ATAGAGAAGA AGCTGCAGGA GGGAGAATCT GCTGGCACTT TGGTTACTTC TCATCAAATC 
ATTATCCAGA AAAGTATCCC ATCATCCCTA GAGGGCACTG AGGATTGGGT TATTATAGAC 
AAATTACCCT CTGAGGTAGT TGATGGTGAA TCGAAAAAAA ATCTGGCATA CAAAGTAGTG 
ACTGTGAGCA GTAAAACTTC CTTTCCACCT GAGATATTAA AATCCAGTAC CATTCTAATG 
CAGAGCTTTG AGGACTTGGA AAGTGAAATA CAATCCAAAG AGGAGAATAA GCAGCAAATG 
TTCACCCTAG GAAAGTCCTA TGATACTGTA TCCGGGAAAA TTGTTACCGT GGCAAGTAAA 
GCTAAAGAGG GTGAGAAAAC AGTACAGCCT TCAGTAGAAG CTTTGCAAAA AATGGAAAGG 
GAAGTGCAAG AATCTGTGAA AATCATTCCG GTAGTGGCTG AGTATGAAAT CCTCGAGCCT 
GTCACTGATG AGAAAGCCAG GCGGGGATCC GACGTGCAGA GCACGAAAAG AAAGCTGTCC 
GACTCCCTGA CACCCATAAA GGAAGCAGAA TCTCAGTTGC AAAGTCCTGA AGAGGAGAGT 
TTGAAAAAGA CCCTAAAGAT GGACCAGGAT GTGCAGTTAC ATGGTACACT GGGAAGCTTG 
CAGCTTGGCA AAGCAGAAAA ACACCTTGGC AGTGAAGCTG TAAAAGCAGG GGCGTTTGCC 
CGGAGAGATA AGAGCCTGTC TGAGTGGAGA TATTCCAGAG AACAGCCATT TACCATTGCT 
ACTGCTCACT ATGTTACCGA GTCGTCTGCA TCAAAAGTAG TGACTAAGCA GTCCACCGGT 
GAAAAAACCT TGGATGGTTC AGACATCTTC AGTTTAATAG AGTCCGCCAG AAAACCGACT 
GAGTTCATAG GAGGGGTTAC TTCTACTTCA CATAGCTGGG CTCAGAGAAT AGACACGACA 
ACATCTGAGG AGGTAACTTC CAGTGAAACG AAGCAGGCCG TTCAGCCGCA TCAGGACACG 
GTGAAGAAGG TTGTCCAGGA GACCGTGATT GTGGAGGAGC GACACGGGAT GAACGTGCAG 
GCGAGCGGGG ACCCTGCTAA AGCGGCTGGC CTTGCGCTGG ATGCCCAGGC GGAGGCTGCT 
GGTGCCAAGG GCAAGGAGGG CTCTGCTGGG ACTGAGGGGG AGAAGGAGGG AAAAAGGGAA 
GAGGCTGGGA AAGCCCCCAC CAAACTGGAA GGAGTCGCTG CTGCTTCGAG AGAGCAGGTG 
GAGGAGCACG CTACAACAGG CCACCTTGCA GAGTCTTTGG AAAGAAAATC TCACTTTGAG 
TCGCCAGTTG TGAAAACTGA GACTATCAGT TTCAGTAGCG TTTCTGCTGG TGGGGAAAAC 
CTTGAAATTG CAACAAAGGA AGTGCCAGTA GTCCATACAG AAACAAAAAC CATTACATAT 
GAGTCTTCTC AGGTGGATTG TGGTGCTGAC TCAGAACCGG GTGTATTGAT GAGTGCACAG 
ACTATCACAT CTGAAACCAC CAGCACTACA ACCACTACAC ATATCACCAA AACTGTGAAA 
GGAGGCATCT CAGAGACAAG AATCGAAAAA CGAATAGTCA TCACAGGAGA TGCAGATATT 
GATCACGATC AGGCGCTGGC TCAGGCAATT AAAGAAGCCA AAGAGCAGCA CCCTGACATG 
TCAGTGACCA AAGTAGTGGT ACATAAAGAG ACAGAAATCA CACCAGAAGA TGGGGAGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794013.1
CDS	114..4436
Translation	MTTESGSDSESKDKEQDQQESPAQQGAAETQPQEQQEQQQQSEEHQNALEQFSAVAAHSTPVKKEQATDKEQEFAAASAKQLEYQQLEDDKLSQKSSSSKLSKSPLKIVKKPKNMQCKVTLLDGSEYACEVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDTENQKNWLDPAKEIKKQIRSGAWQFAFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDYDPDEYGSDYVSEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRASALIDRPAPYFERSSSKRYTMSRSLDGASVNENHEMYMKDSVSAAEVGTGQYATTKGISQTNLITTVTPEKKAEEEKDDEEGKKKRAEEVTPVSAIRHDTKPSLLGPDSRHRHPPPSSPPGPHAPPAKLRRRCKESAGAKPAGGEGPKESAPRHGEPEPDSDRKGRVCSPEQDVPFAYKQKAGKGGTLFSFSLQLPESFPSLLDDDGYLSLPNLSETNLLPASVQHYLPIRSPSLVPCFLFIFFFLLSASFSVPYALTLSFPLAMCLCYLEPKAASLSASLANDLSDSSEEETDSEQTDTAADGETTATESDQDEDGDLKAQNSLIKRIQGENVYVKHSNLMLEDLDKTQEDLMKHQTNISELKRTFLETSTETTVSNEWEKRLSTSPVRLAARQEEAPMIEPLVPEETKEEREKSEKLIFLQKGGTTFLELQPSVIEKKLQEGESAGTLVTSHQIIIQKSIPSSLEGTEDWVIIDKLPSEVVDGESKKNLAYKVVTVSSKTSFPPEILKSSTILMQSFEDLESEIQSKEENKQQMFTLGKSYDTVSGKIVTVASKAKEGEKTVQPSVEALQKMEREVQESVKIIPVVAEYEILEPVTDEKARRGSDVQSTKRKLSDSLTPIKEAESQLQSPEEESLKKTLKMDQDVQLHGTLGSLQLGKAEKHLGSEAVKAGAFARRDKSLSEWRYSREQPFTIATAHYVTESSASKVVTKQSTGEKTLDGSDIFSLIESARKPTEFIGGVTSTSHSWAQRIDTTTSEEVTSSETKQAVQPHQDTVKKVVQETVIVEERHGMNVQASGDPAKAAGLALDAQAEAAGAKGKEGSAGTEGEKEGKREEAGKAPTKLEGVAAASREQVEEHATTGHLAESLERKSHFESPVVKTETISFSSVSAGGENLEIATKEVPVVHTETKTITYESSQVDCGADSEPGVLMSAQTITSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEITPEDGED

Target ORF information:

RefSeq Version	XM_014938527.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014938527.1

ORF Insert Sequence:

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGCCATC TTTGCTTGGC CCCGACTCCC GCCACCGCCA CCCCCCGCCA 
TCCTCGCCGC CTGGCCCCCA CGCACCTCCC GCCAAGCTCC GCAGGAGATG CAAGGAGAGC 
GCTGGAGCAA AGCCCGCGGG GGGCGAGGGG CCCAAGGAGA GCGCTCCCCG GCACGGCGAG 
CCGGAGCCCG ACTCCGACAG AAAGGGGCGA GTTTGCTCCC CCGAGCAAGA CGTGCCTTTC 
GCCTATAAGC AAAAAGCCGG CAAAGGCGGA ACGCTGTTTT CCTTCTCCTT GCAACTCCCG 
GAGTCCTTTC CTTCCCTCCT GGATGACGAC GGCTACCTGT CGCTCCCTAA CCTCTCCGAA 
ACCAACCTCC TGCCTGCCAG CGTGCAGCAC TACCTTCCCA TCCGCTCCCC CTCCCTGGTG 
CCTTGCTTCC TCTTCATTTT TTTCTTTCTG CTCTCTGCCT CTTTCTCAGT GCCATACGCC 
CTCACTCTCT CCTTCCCTCT GGCTATGTGC CTCTGCTACC TGGAGCCCAA GGCGGCCTCC 
TTGAGTGCCT CACTTGCCAA TGACCTGAGT GACAGTTCAG AGGAAGAGAC TGACAGTGAG 
CAGACGGACA CTGCAGCTGA TGGTGAGACC ACTGCCACCG AGTCGGATCA AGACGAGGAC 
GGAGATCTAA AGGCACAGAA TAGTCTGATT AAGCGAATAC AGGGTGAAAA TGTGTATGTC 
AAACATAGTA ATCTTATGTT AGAGGACCTA GACAAAACCC AGGAAGATCT GATGAAACAT 
CAGACCAATA TAAGCGAGCT GAAACGCACC TTCTTGGAAA CCTCCACAGA AACAACTGTG 
TCTAATGAGT GGGAGAAGAG GCTTTCCACA TCTCCCGTTC GGCTTGCAGC AAGGCAGGAG 
GAAGCTCCTA TGATTGAACC ACTAGTGCCT GAAGAGACCA AAGAAGAAAG AGAAAAATCA 
GAAAAGCTCA TATTTTTGCA GAAGGGAGGT ACTACATTCC TTGAATTGCA GCCAAGTGTG 
ATAGAGAAGA AGCTGCAGGA GGGAGAATCT GCTGGCACTT TGGTTACTTC TCATCAAATC 
ATTATCCAGA AAAGTATCCC ATCATCCCTA GAGGGCACTG AGGATTGGGT TATTATAGAC 
AAATTACCCT CTGAGGTAGT TGATGGTGAA TCGAAAAAAA ATCTGGCATA CAAAGTAGTG 
ACTGTGAGCA GTAAAACTTC CTTTCCACCT GAGATATTAA AATCCAGTAC CATTCTAATG 
CAGAGCTTTG AGGACTTGGA AAGTGAAATA CAATCCAAAG AGGAGAATAA GCAGCAAATG 
TTCACCCTAG GAAAGTCCTA TGATACTGTA TCCGGGAAAA TTGTTACCGT GGCAAGTAAA 
GCTAAAGAGG GTGAGAAAAC AGTACAGCCT TCAGTAGAAG CTTTGCAAAA AATGGAAAGG 
GAAGTGCAAG AATCTGTGAA AATCATTCCG GTAGTGGCTG AGTATGAAAT CCTCGAGCCT 
GTCACTGATG AGAAAGCCAG GCGGGGATCC GACGTGCAGA GCACGAAAAG AAAGCTGTCC 
GACTCCCTGA CACCCATAAA GGAAGCAGAA TCTCAGTTGC AAAGTCCTGA AGAGGAGAGT 
TTGAAAAAGA CCCTAAAGAT GGACCAGGAT GTGCAGTTAC ATGGTACACT GGGAAGCTTG 
CAGCTTGGCA AAGCAGAAAA ACACCTTGGC AGTGAAGCTG TAAAAGCAGG GGCGTTTGCC 
CGGAGAGATA AGAGCCTGTC TGAGTGGAGA TATTCCAGAG AACAGCCATT TACCATTGCT 
ACTGCTCACT ATGTTACCGA GTCGTCTGCA TCAAAAGTAG TGACTAAGCA GTCCACCGGT 
GAAAAAACCT TGGATGGTTC AGACATCTTC AGTTTAATAG AGTCCGCCAG AAAACCGACT 
GAGTTCATAG GAGGGGTTAC TTCTACTTCA CATAGCTGGG CTCAGAGAAT AGACACGACA 
ACATCTGAGG AGGTAACTTC CAGTGAAACG AAGCAGGCCG TTCAGCCGCA TCAGGACACG 
GTGAAGAAGG TTGTCCAGGA GACCGTGATT GTGGAGGAGC GACACGGGAT GAACGTGCAG 
GCGAGCGGGG ACCCTGCTAA AGCGGCTGGC CTTGCGCTGG ATGCCCAGGC GGAGGCTGCT 
GGTGCCAAGG GCAAGGAGGG CTCTGCTGGG ACTGAGGGGG AGAAGGAGGG AAAAAGGGAA 
GAGGCTGGGA AAGCCCCCAC CAAACTGGAA GGAGTCGCTG CTGCTTCGAG AGAGCAGGTG 
GAGGAGCACG CTACAACAGG CCACCTTGCA GAGTCTTTGG AAAGAAAATC TCACTTTGAG 
TCGCCAGTTG TGAAAACTGA GACTATCAGT TTCAGTAGCG TTTCTGCTGG TGGGGAAAAC 
CTTGAAATTG CAACAAAGGA AGTGCCAGTA GTCCATACAG AAACAAAAAC CATTACATAT 
GAGTCTTCTC AGGTGGATTG TGGTGCTGAC TCAGAACCGG GTGTATTGAT GAGTGCACAG 
ACTATCACAT CTGAAACCAC CAGCACTACA ACCACTACAC ATATCACCAA AACTGTGAAA 
GGAGGCATCT CAGAGACAAG AATCGAAAAA CGAATAGTCA TCACAGGAGA TGCAGATATT 
GATCACGATC AGGCGCTGGC TCAGGCAATT AAAGAAGCCA AAGAGCAGCA CCCTGACATG 
TCAGTGACCA AAGTAGTGGT ACATAAAGAG ACAGAAATCA CACCAGAAGA TGGGGAGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPB41L3 ( XM_014938528.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794014.1 Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X2, mRNA.

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CloneID	OCa62518
Clone ID Related Accession (Same CDS sequence)	XM_014938528.1
Accession Version	XM_014938528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	band 4.1-like protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGCCATC TTTGCTTGGC CCCGACTCCC GCCACCGCCA CCCCCCGCCA 
TCCTCGCCGC CTGGCCCCCA CGCACCTCCC GCCAAGCTCC GCAGGAGATG CAAGGAGAGC 
GCTGGAGCAA AGCCCGCGGG GGGCGAGGGG CCCAAGGAGA GCGCTCCCCG GCACGGCGAG 
CCGGAGCCCG ACTCCGACAG AAAGGGGCGA GTTTGCTCCC CCGAGCAAGA CGTGCCTTTC 
GCCTATAAGC AAAAAGCCGG CAAAGGCGGA ACGCTGTTTT CCTTCTCCTT GCAACTCCCG 
GAGTCCTTTC CTTCCCTCCT GGATGACGAC GGCTACCTGT CGCTCCCTAA CCTCTCCGAA 
ACCAACCTCC TGCCTGCCAG CGTGCAGCAC TACCTTCCCA TCCGCTCCCC CTCCCTGGTG 
CCTTGCTTCC TCTTCATTTT TTTCTTTCTG CTCTCTGCCT CTTTCTCAGT GCCATACGCC 
CTCACTCTCT CCTTCCCTCT GGCTATGTGC CTCTGCTACC TGGAGCCCAA GGCGGCCTCC 
TTGAGTGCCT CACTTGCCAA TGACCTGAGT GACAGTTCAG AGGAAGAGAC TGACAGTGAG 
CAGACGGACA CTGCAGCTGA TGGTGAGACC ACTGCCACCG AGTCGGATCA AGACGAGGAC 
GGAGATCTAA AGGCACAGAA TAGTCTGATT AAGCGAATAC AGGGTGAAAA TGTGTATGTC 
AAACATAGTA ATCTTATGTT AGAGGACCTA GACAAAACCC AGGAAGATCT GATGAAACAT 
CAGACCAATA TAAGCGAGCT GAAACGCACC TTCTTGGAAA CCTCCACAGA AACAACTGTG 
TCTAATGAGT GGGAGAAGAG GCTTTCCACA TCTCCCGTTC GGCTTGCAGC AAGGCAGGAG 
GAAGCTCCTA TGATTGAACC ACTAGTGCCT GAAGAGACCA AAGAAGAAAG AGAAAAATCA 
GAAAAGCTCA TATTTTTGCA GAAGGGAGGT ACTACATTCC TTGAATTGCA GCCAAGTGTG 
ATAGAGAAGA AGCTGCAGGA GGGAGAATCT GCTGGCACTT TGGTTACTTC TCATCAAATC 
ATTATCCAGA AAAGTATCCC ATCATCCCTA GAGAGCTTTG AGGACTTGGA AAGTGAAATA 
CAATCCAAAG AGGAGAATAA GCAGCAAATG TTCACCCTAG GAAAGTCCTA TGATACTGTA 
TCCGGGAAAA TTGTTACCGT GGCAAGTAAA GCTAAAGAGG GTGAGAAAAC AGTACAGCCT 
TCAGTAGAAG CTTTGCAAAA AATGGAAAGG GAAGTGCAAG AATCTGTGAA AATCATTCCG 
GTAGTGGCTG AGTATGAAAT CCTCGAGCCT GTCACTGATG AGAAAGCCAG GCGGGGATCC 
GACGTGCAGA GCACGAAAAG AAAGCTGTCC GACTCCCTGA CACCCATAAA GGAAGCAGAA 
TCTCAGTTGC AAAGTCCTGA AGAGGAGAGT TTGAAAAAGA CCCTAAAGAT GGACCAGGAT 
GTGCAGTTAC ATGGTACACT GGGAAGCTTG CAGCTTGGCA AAGCAGAAAA ACACCTTGGC 
AGTGAAGCTG TAAAAGCAGG GGCGTTTGCC CGGAGAGATA AGAGCCTGTC TGAGTGGAGA 
TATTCCAGAG AACAGCCATT TACCATTGCT ACTGCTCACT ATGTTACCGA GTCGTCTGCA 
TCAAAAGTAG TGACTAAGCA GTCCACCGGT GAAAAAACCT TGGATGGTTC AGACATCTTC 
AGTTTAATAG AGTCCGCCAG AAAACCGACT GAGTTCATAG GAGGGGTTAC TTCTACTTCA 
CATAGCTGGG CTCAGAGAAT AGACACGACA ACATCTGAGG AGGTAACTTC CAGTGAAACG 
AAGCAGGCCG TTCAGCCGCA TCAGGACACG GTGAAGAAGG TTGTCCAGGA GACCGTGATT 
GTGGAGGAGC GACACGGGAT GAACGTGCAG GCGAGCGGGG ACCCTGCTAA AGCGGCTGGC 
CTTGCGCTGG ATGCCCAGGC GGAGGCTGCT GGTGCCAAGG GCAAGGAGGG CTCTGCTGGG 
ACTGAGGGGG AGAAGGAGGG AAAAAGGGAA GAGGCTGGGA AAGCCCCCAC CAAACTGGAA 
GGAGTCGCTG CTGCTTCGAG AGAGCAGGTG GAGGAGCACG CTACAACAGG CCACCTTGCA 
GAGTCTTTGG AAAGAAAATC TCACTTTGAG TCGCCAGTTG TGAAAACTGA GACTATCAGT 
TTCAGTAGCG TTTCTGCTGG TGGGGAAAAC CTTGAAATTG CAACAAAGGA AGTGCCAGTA 
GTCCATACAG AAACAAAAAC CATTACATAT GAGTCTTCTC AGGTGGATTG TGGTGCTGAC 
TCAGAACCGG GTGTATTGAT GAGTGCACAG ACTATCACAT CTGAAACCAC CAGCACTACA 
ACCACTACAC ATATCACCAA AACTGTGAAA GGAGGCATCT CAGAGACAAG AATCGAAAAA 
CGAATAGTCA TCACAGGAGA TGCAGATATT GATCACGATC AGGCGCTGGC TCAGGCAATT 
AAAGAAGCCA AAGAGCAGCA CCCTGACATG TCAGTGACCA AAGTAGTGGT ACATAAAGAG 
ACAGAAATCA CACCAGAAGA TGGGGAGGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794014.1
CDS	150..4322
Translation	MTTESGSDSESKDKEQDQQESPAQQGAAETQPQEQQEQQQQSEEHQNALEQFSAVAAHSTPVKKEQATDKEQEFAAASAKQLEYQQLEDDKLSQKSSSSKLSKSPLKIVKKPKNMQCKVTLLDGSEYACEVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDTENQKNWLDPAKEIKKQIRSGAWQFAFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDYDPDEYGSDYVSEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRASALIDRPAPYFERSSSKRYTMSRSLDGASVNENHEMYMKDSVSAAEVGTGQYATTKGISQTNLITTVTPEKKAEEEKDDEEGKKKRAEEVTPVSAIRHDTKPSLLGPDSRHRHPPPSSPPGPHAPPAKLRRRCKESAGAKPAGGEGPKESAPRHGEPEPDSDRKGRVCSPEQDVPFAYKQKAGKGGTLFSFSLQLPESFPSLLDDDGYLSLPNLSETNLLPASVQHYLPIRSPSLVPCFLFIFFFLLSASFSVPYALTLSFPLAMCLCYLEPKAASLSASLANDLSDSSEEETDSEQTDTAADGETTATESDQDEDGDLKAQNSLIKRIQGENVYVKHSNLMLEDLDKTQEDLMKHQTNISELKRTFLETSTETTVSNEWEKRLSTSPVRLAARQEEAPMIEPLVPEETKEEREKSEKLIFLQKGGTTFLELQPSVIEKKLQEGESAGTLVTSHQIIIQKSIPSSLESFEDLESEIQSKEENKQQMFTLGKSYDTVSGKIVTVASKAKEGEKTVQPSVEALQKMEREVQESVKIIPVVAEYEILEPVTDEKARRGSDVQSTKRKLSDSLTPIKEAESQLQSPEEESLKKTLKMDQDVQLHGTLGSLQLGKAEKHLGSEAVKAGAFARRDKSLSEWRYSREQPFTIATAHYVTESSASKVVTKQSTGEKTLDGSDIFSLIESARKPTEFIGGVTSTSHSWAQRIDTTTSEEVTSSETKQAVQPHQDTVKKVVQETVIVEERHGMNVQASGDPAKAAGLALDAQAEAAGAKGKEGSAGTEGEKEGKREEAGKAPTKLEGVAAASREQVEEHATTGHLAESLERKSHFESPVVKTETISFSSVSAGGENLEIATKEVPVVHTETKTITYESSQVDCGADSEPGVLMSAQTITSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEITPEDGED

Target ORF information:

RefSeq Version	XM_014938528.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014938528.1

ORF Insert Sequence:

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGCCATC TTTGCTTGGC CCCGACTCCC GCCACCGCCA CCCCCCGCCA 
TCCTCGCCGC CTGGCCCCCA CGCACCTCCC GCCAAGCTCC GCAGGAGATG CAAGGAGAGC 
GCTGGAGCAA AGCCCGCGGG GGGCGAGGGG CCCAAGGAGA GCGCTCCCCG GCACGGCGAG 
CCGGAGCCCG ACTCCGACAG AAAGGGGCGA GTTTGCTCCC CCGAGCAAGA CGTGCCTTTC 
GCCTATAAGC AAAAAGCCGG CAAAGGCGGA ACGCTGTTTT CCTTCTCCTT GCAACTCCCG 
GAGTCCTTTC CTTCCCTCCT GGATGACGAC GGCTACCTGT CGCTCCCTAA CCTCTCCGAA 
ACCAACCTCC TGCCTGCCAG CGTGCAGCAC TACCTTCCCA TCCGCTCCCC CTCCCTGGTG 
CCTTGCTTCC TCTTCATTTT TTTCTTTCTG CTCTCTGCCT CTTTCTCAGT GCCATACGCC 
CTCACTCTCT CCTTCCCTCT GGCTATGTGC CTCTGCTACC TGGAGCCCAA GGCGGCCTCC 
TTGAGTGCCT CACTTGCCAA TGACCTGAGT GACAGTTCAG AGGAAGAGAC TGACAGTGAG 
CAGACGGACA CTGCAGCTGA TGGTGAGACC ACTGCCACCG AGTCGGATCA AGACGAGGAC 
GGAGATCTAA AGGCACAGAA TAGTCTGATT AAGCGAATAC AGGGTGAAAA TGTGTATGTC 
AAACATAGTA ATCTTATGTT AGAGGACCTA GACAAAACCC AGGAAGATCT GATGAAACAT 
CAGACCAATA TAAGCGAGCT GAAACGCACC TTCTTGGAAA CCTCCACAGA AACAACTGTG 
TCTAATGAGT GGGAGAAGAG GCTTTCCACA TCTCCCGTTC GGCTTGCAGC AAGGCAGGAG 
GAAGCTCCTA TGATTGAACC ACTAGTGCCT GAAGAGACCA AAGAAGAAAG AGAAAAATCA 
GAAAAGCTCA TATTTTTGCA GAAGGGAGGT ACTACATTCC TTGAATTGCA GCCAAGTGTG 
ATAGAGAAGA AGCTGCAGGA GGGAGAATCT GCTGGCACTT TGGTTACTTC TCATCAAATC 
ATTATCCAGA AAAGTATCCC ATCATCCCTA GAGAGCTTTG AGGACTTGGA AAGTGAAATA 
CAATCCAAAG AGGAGAATAA GCAGCAAATG TTCACCCTAG GAAAGTCCTA TGATACTGTA 
TCCGGGAAAA TTGTTACCGT GGCAAGTAAA GCTAAAGAGG GTGAGAAAAC AGTACAGCCT 
TCAGTAGAAG CTTTGCAAAA AATGGAAAGG GAAGTGCAAG AATCTGTGAA AATCATTCCG 
GTAGTGGCTG AGTATGAAAT CCTCGAGCCT GTCACTGATG AGAAAGCCAG GCGGGGATCC 
GACGTGCAGA GCACGAAAAG AAAGCTGTCC GACTCCCTGA CACCCATAAA GGAAGCAGAA 
TCTCAGTTGC AAAGTCCTGA AGAGGAGAGT TTGAAAAAGA CCCTAAAGAT GGACCAGGAT 
GTGCAGTTAC ATGGTACACT GGGAAGCTTG CAGCTTGGCA AAGCAGAAAA ACACCTTGGC 
AGTGAAGCTG TAAAAGCAGG GGCGTTTGCC CGGAGAGATA AGAGCCTGTC TGAGTGGAGA 
TATTCCAGAG AACAGCCATT TACCATTGCT ACTGCTCACT ATGTTACCGA GTCGTCTGCA 
TCAAAAGTAG TGACTAAGCA GTCCACCGGT GAAAAAACCT TGGATGGTTC AGACATCTTC 
AGTTTAATAG AGTCCGCCAG AAAACCGACT GAGTTCATAG GAGGGGTTAC TTCTACTTCA 
CATAGCTGGG CTCAGAGAAT AGACACGACA ACATCTGAGG AGGTAACTTC CAGTGAAACG 
AAGCAGGCCG TTCAGCCGCA TCAGGACACG GTGAAGAAGG TTGTCCAGGA GACCGTGATT 
GTGGAGGAGC GACACGGGAT GAACGTGCAG GCGAGCGGGG ACCCTGCTAA AGCGGCTGGC 
CTTGCGCTGG ATGCCCAGGC GGAGGCTGCT GGTGCCAAGG GCAAGGAGGG CTCTGCTGGG 
ACTGAGGGGG AGAAGGAGGG AAAAAGGGAA GAGGCTGGGA AAGCCCCCAC CAAACTGGAA 
GGAGTCGCTG CTGCTTCGAG AGAGCAGGTG GAGGAGCACG CTACAACAGG CCACCTTGCA 
GAGTCTTTGG AAAGAAAATC TCACTTTGAG TCGCCAGTTG TGAAAACTGA GACTATCAGT 
TTCAGTAGCG TTTCTGCTGG TGGGGAAAAC CTTGAAATTG CAACAAAGGA AGTGCCAGTA 
GTCCATACAG AAACAAAAAC CATTACATAT GAGTCTTCTC AGGTGGATTG TGGTGCTGAC 
TCAGAACCGG GTGTATTGAT GAGTGCACAG ACTATCACAT CTGAAACCAC CAGCACTACA 
ACCACTACAC ATATCACCAA AACTGTGAAA GGAGGCATCT CAGAGACAAG AATCGAAAAA 
CGAATAGTCA TCACAGGAGA TGCAGATATT GATCACGATC AGGCGCTGGC TCAGGCAATT 
AAAGAAGCCA AAGAGCAGCA CCCTGACATG TCAGTGACCA AAGTAGTGGT ACATAAAGAG 
ACAGAAATCA CACCAGAAGA TGGGGAGGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPB41L3 ( XM_014938529.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794015.1 Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCa62519
Clone ID Related Accession (Same CDS sequence)	XM_014938529.1
Accession Version	XM_014938529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	band 4.1-like protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGTCGGA TCAAGACGAG GACGGAGATC TAAAGGCACA GAATAGTCTG 
ATTAAGCGAA TACAGGGTGA AAATGTGTAT GTCAAACATA GTAATCTTAT GTTAGAGGAC 
CTAGACAAAA CCCAGGAAGA TCTGATGAAA CATCAGACCA ATATAAGCGA GCTGAAACGC 
ACCTTCTTGG AAACCTCCAC AGAAACAACT GTGTCTAATG AGTGGGAGAA GAGGCTTTCC 
ACATCTCCCG TTCGGCTTGC AGCAAGGCAG GAGGAAGCTC CTATGATTGA ACCACTAGTG 
CCTGAAGAGA CCAAAGAAGA AAGAGAAAAA TCAGAAAAGC TCATATTTTT GCAGAAGGGA 
GGTACTACAT TCCTTGAATT GCAGCCAAGT GTGATAGAGA AGAAGCTGCA GGAGGGAGAA 
TCTGCTGGCA CTTTGGTTAC TTCTCATCAA ATCATTATCC AGAAAAGTAT CCCATCATCC 
CTAGAGGGCA CTGAGGATTG GGTTATTATA GACAAATTAC CCTCTGAGGT AGTTGATGGT 
GAATCGAAAA AAAATCTGGC ATACAAAGTA GTGACTGTGA GCAGTAAAAC TTCCTTTCCA 
CCTGAGATAT TAAAATCCAG TACCATTCTA ATGCAGAGCT TTGAGGACTT GGAAAGTGAA 
ATACAATCCA AAGAGGAGAA TAAGCAGCAA ATGTTCACCC TAGGAAAGTC CTATGATACT 
GTATCCGGGA AAATTGTTAC CGTGGCAAGT AAAGCTAAAG AGGGTGAGAA AACAGTACAG 
CCTTCAGTAG AAGCTTTGCA AAAAATGGAA AGGGAAGTGC AAGAATCTGT GAAAATCATT 
CCGGTAGTGG CTGAGTATGA AATCCTCGAG CCTGTCACTG ATGAGAAAGC CAGGCGGGGA 
TCCGACGTGC AGAGCACGAA AAGAAAGCTG TCCGACTCCC TGACACCCAT AAAGGAAGCA 
GAATCTCAGT TGCAAAGTCC TGAAGAGGAG AGTTTGAAAA AGACCCTAAA GATGGACCAG 
GATGTGCAGT TACATGGTAC ACTGGGAAGC TTGCAGCTTG GCAAAGCAGA AAAACACCTT 
GGCAGTGAAG CTGTAAAAGC AGGGGCGTTT GCCCGGAGAG ATAAGAGCCT GTCTGAGTGG 
AGATATTCCA GAGAACAGCC ATTTACCATT GCTACTGCTC ACTATGTTAC CGAGTCGTCT 
GCATCAAAAG TAGTGACTAA GCAGTCCACC GGTGAAAAAA CCTTGGATGG TTCAGACATC 
TTCAGTTTAA TAGAGTCCGC CAGAAAACCG ACTGAGTTCA TAGGAGGGGT TACTTCTACT 
TCACATAGCT GGGCTCAGAG AATAGACACG ACAACATCTG AGGAGGTAAC TTCCAGTGAA 
ACGAAGCAGG CCGTTCAGCC GCATCAGGAC ACGGTGAAGA AGGTTGTCCA GGAGACCGTG 
ATTGTGGAGG AGCGACACGG GATGAACGTG CAGGCGAGCG GGGACCCTGC TAAAGCGGCT 
GGCCTTGCGC TGGATGCCCA GGCGGAGGCT GCTGGTGCCA AGGGCAAGGA GGGCTCTGCT 
GGGACTGAGG GGGAGAAGGA GGGAAAAAGG GAAGAGGCTG GGAAAGCCCC CACCAAACTG 
GAAGGAGTCG CTGCTGCTTC GAGAGAGCAG GTGGAGGAGC ACGCTACAAC AGGCCACCTT 
GCAGAGTCTT TGGAAAGAAA ATCTCACTTT GAGTCGCCAG TTGTGAAAAC TGAGACTATC 
AGTTTCAGTA GCGTTTCTGC TGGTGGGGAA AACCTTGAAA TTGCAACAAA GGAAGTGCCA 
GTAGTCCATA CAGAAACAAA AACCATTACA TATGAGTCTT CTCAGGTGGA TTGTGGTGCT 
GACTCAGAAC CGGGTGTATT GATGAGTGCA CAGACTATCA CATCTGAAAC CACCAGCACT 
ACAACCACTA CACATATCAC CAAAACTGTG AAAGGAGGCA TCTCAGAGAC AAGAATCGAA 
AAACGAATAG TCATCACAGG AGATGCAGAT ATTGATCACG ATCAGGCGCT GGCTCAGGCA 
ATTAAAGAAG CCAAAGAGCA GCACCCTGAC ATGTCAGTGA CCAAAGTAGT GGTACATAAA 
GAGACAGAAA TCACACCAGA AGATGGGGAG GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794015.1
CDS	150..3845
Translation	MTTESGSDSESKDKEQDQQESPAQQGAAETQPQEQQEQQQQSEEHQNALEQFSAVAAHSTPVKKEQATDKEQEFAAASAKQLEYQQLEDDKLSQKSSSSKLSKSPLKIVKKPKNMQCKVTLLDGSEYACEVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDTENQKNWLDPAKEIKKQIRSGAWQFAFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDYDPDEYGSDYVSEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRASALIDRPAPYFERSSSKRYTMSRSLDGASVNENHEMYMKDSVSAAEVGTGQYATTKGISQTNLITTVTPEKKAEEEKDDEEGKKKRAEEVTPVSAIRHDTKSDQDEDGDLKAQNSLIKRIQGENVYVKHSNLMLEDLDKTQEDLMKHQTNISELKRTFLETSTETTVSNEWEKRLSTSPVRLAARQEEAPMIEPLVPEETKEEREKSEKLIFLQKGGTTFLELQPSVIEKKLQEGESAGTLVTSHQIIIQKSIPSSLEGTEDWVIIDKLPSEVVDGESKKNLAYKVVTVSSKTSFPPEILKSSTILMQSFEDLESEIQSKEENKQQMFTLGKSYDTVSGKIVTVASKAKEGEKTVQPSVEALQKMEREVQESVKIIPVVAEYEILEPVTDEKARRGSDVQSTKRKLSDSLTPIKEAESQLQSPEEESLKKTLKMDQDVQLHGTLGSLQLGKAEKHLGSEAVKAGAFARRDKSLSEWRYSREQPFTIATAHYVTESSASKVVTKQSTGEKTLDGSDIFSLIESARKPTEFIGGVTSTSHSWAQRIDTTTSEEVTSSETKQAVQPHQDTVKKVVQETVIVEERHGMNVQASGDPAKAAGLALDAQAEAAGAKGKEGSAGTEGEKEGKREEAGKAPTKLEGVAAASREQVEEHATTGHLAESLERKSHFESPVVKTETISFSSVSAGGENLEIATKEVPVVHTETKTITYESSQVDCGADSEPGVLMSAQTITSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEITPEDGED

Target ORF information:

RefSeq Version	XM_014938529.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014938529.1

ORF Insert Sequence:

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGTCGGA TCAAGACGAG GACGGAGATC TAAAGGCACA GAATAGTCTG 
ATTAAGCGAA TACAGGGTGA AAATGTGTAT GTCAAACATA GTAATCTTAT GTTAGAGGAC 
CTAGACAAAA CCCAGGAAGA TCTGATGAAA CATCAGACCA ATATAAGCGA GCTGAAACGC 
ACCTTCTTGG AAACCTCCAC AGAAACAACT GTGTCTAATG AGTGGGAGAA GAGGCTTTCC 
ACATCTCCCG TTCGGCTTGC AGCAAGGCAG GAGGAAGCTC CTATGATTGA ACCACTAGTG 
CCTGAAGAGA CCAAAGAAGA AAGAGAAAAA TCAGAAAAGC TCATATTTTT GCAGAAGGGA 
GGTACTACAT TCCTTGAATT GCAGCCAAGT GTGATAGAGA AGAAGCTGCA GGAGGGAGAA 
TCTGCTGGCA CTTTGGTTAC TTCTCATCAA ATCATTATCC AGAAAAGTAT CCCATCATCC 
CTAGAGGGCA CTGAGGATTG GGTTATTATA GACAAATTAC CCTCTGAGGT AGTTGATGGT 
GAATCGAAAA AAAATCTGGC ATACAAAGTA GTGACTGTGA GCAGTAAAAC TTCCTTTCCA 
CCTGAGATAT TAAAATCCAG TACCATTCTA ATGCAGAGCT TTGAGGACTT GGAAAGTGAA 
ATACAATCCA AAGAGGAGAA TAAGCAGCAA ATGTTCACCC TAGGAAAGTC CTATGATACT 
GTATCCGGGA AAATTGTTAC CGTGGCAAGT AAAGCTAAAG AGGGTGAGAA AACAGTACAG 
CCTTCAGTAG AAGCTTTGCA AAAAATGGAA AGGGAAGTGC AAGAATCTGT GAAAATCATT 
CCGGTAGTGG CTGAGTATGA AATCCTCGAG CCTGTCACTG ATGAGAAAGC CAGGCGGGGA 
TCCGACGTGC AGAGCACGAA AAGAAAGCTG TCCGACTCCC TGACACCCAT AAAGGAAGCA 
GAATCTCAGT TGCAAAGTCC TGAAGAGGAG AGTTTGAAAA AGACCCTAAA GATGGACCAG 
GATGTGCAGT TACATGGTAC ACTGGGAAGC TTGCAGCTTG GCAAAGCAGA AAAACACCTT 
GGCAGTGAAG CTGTAAAAGC AGGGGCGTTT GCCCGGAGAG ATAAGAGCCT GTCTGAGTGG 
AGATATTCCA GAGAACAGCC ATTTACCATT GCTACTGCTC ACTATGTTAC CGAGTCGTCT 
GCATCAAAAG TAGTGACTAA GCAGTCCACC GGTGAAAAAA CCTTGGATGG TTCAGACATC 
TTCAGTTTAA TAGAGTCCGC CAGAAAACCG ACTGAGTTCA TAGGAGGGGT TACTTCTACT 
TCACATAGCT GGGCTCAGAG AATAGACACG ACAACATCTG AGGAGGTAAC TTCCAGTGAA 
ACGAAGCAGG CCGTTCAGCC GCATCAGGAC ACGGTGAAGA AGGTTGTCCA GGAGACCGTG 
ATTGTGGAGG AGCGACACGG GATGAACGTG CAGGCGAGCG GGGACCCTGC TAAAGCGGCT 
GGCCTTGCGC TGGATGCCCA GGCGGAGGCT GCTGGTGCCA AGGGCAAGGA GGGCTCTGCT 
GGGACTGAGG GGGAGAAGGA GGGAAAAAGG GAAGAGGCTG GGAAAGCCCC CACCAAACTG 
GAAGGAGTCG CTGCTGCTTC GAGAGAGCAG GTGGAGGAGC ACGCTACAAC AGGCCACCTT 
GCAGAGTCTT TGGAAAGAAA ATCTCACTTT GAGTCGCCAG TTGTGAAAAC TGAGACTATC 
AGTTTCAGTA GCGTTTCTGC TGGTGGGGAA AACCTTGAAA TTGCAACAAA GGAAGTGCCA 
GTAGTCCATA CAGAAACAAA AACCATTACA TATGAGTCTT CTCAGGTGGA TTGTGGTGCT 
GACTCAGAAC CGGGTGTATT GATGAGTGCA CAGACTATCA CATCTGAAAC CACCAGCACT 
ACAACCACTA CACATATCAC CAAAACTGTG AAAGGAGGCA TCTCAGAGAC AAGAATCGAA 
AAACGAATAG TCATCACAGG AGATGCAGAT ATTGATCACG ATCAGGCGCT GGCTCAGGCA 
ATTAAAGAAG CCAAAGAGCA GCACCCTGAC ATGTCAGTGA CCAAAGTAGT GGTACATAAA 
GAGACAGAAA TCACACCAGA AGATGGGGAG GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPB41L3 ( XM_014938531.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794017.1 Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa62520
Clone ID Related Accession (Same CDS sequence)	XM_014938531.1
Accession Version	XM_014938531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	band 4.1-like protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGAATAG TCTGATTAAG CGAATACAGG GTGAAAATGT GTATGTCAAA 
CATAGTAATC TTATGTTAGA GGACCTAGAC AAAACCCAGG AAGATCTGAT GAAACATCAG 
ACCAATATAA GCGAGCTGAA ACGCACCTTC TTGGAAACCT CCACAGAAAC AACTGTGTCT 
AATGAGTGGG AGAAGAGGCT TTCCACATCT CCCGTTCGGC TTGCAGCAAG GCAGGAGGAA 
GCTCCTATGA TTGAACCACT AGTGCCTGAA GAGACCAAAG AAGAAAGAGA AAAATCAGAA 
AAGCTCATAT TTTTGCAGAA GGGAGGTACT ACATTCCTTG AATTGCAGCC AAGTGTGATA 
GAGAAGAAGC TGCAGGAGGG AGAATCTGCT GGCACTTTGG TTACTTCTCA TCAAATCATT 
ATCCAGAAAA GTATCCCATC ATCCCTAGAG GGCACTGAGG ATTGGGTTAT TATAGACAAA 
TTACCCTCTG AGGTAGTTGA TGGTGAATCG AAAAAAAATC TGGCATACAA AGTAGTGACT 
GTGAGCAGTA AAACTTCCTT TCCACCTGAG ATATTAAAAT CCAGTACCAT TCTAATGCAG 
AGCTTTGAGG ACTTGGAAAG TGAAATACAA TCCAAAGAGG AGAATAAGCA GCAAATGTTC 
ACCCTAGGAA AGTCCTATGA TACTGTATCC GGGAAAATTG TTACCGTGGC AAGTAAAGCT 
AAAGAGGGTG AGAAAACAGT ACAGCCTTCA GTAGAAGCTT TGCAAAAAAT GGAAAGGGAA 
GTGCAAGAAT CTGTGAAAAT CATTCCGGTA GTGGCTGAGT ATGAAATCCT CGAGCCTGTC 
ACTGATGAGA AAGCCAGGCG GGGATCCGAC GTGCAGAGCA CGAAAAGAAA GCTGTCCGAC 
TCCCTGACAC CCATAAAGGA AGCAGAATCT CAGTTGCAAA GTCCTGAAGA GGAGAGTTTG 
AAAAAGACCC TAAAGATGGA CCAGGATGTG CAGTTACATG GTACACTGGG AAGCTTGCAG 
CTTGGCAAAG CAGAAAAACA CCTTGGCAGT GAAGCTGTAA AAGCAGGGGC GTTTGCCCGG 
AGAGATAAGA GCCTGTCTGA GTGGAGATAT TCCAGAGAAC AGCCATTTAC CATTGCTACT 
GCTCACTATG TTACCGAGTC GTCTGCATCA AAAGTAGTGA CTAAGCAGTC CACCGGTGAA 
AAAACCTTGG ATGGTTCAGA CATCTTCAGT TTAATAGAGT CCGCCAGAAA ACCGACTGAG 
TTCATAGGAG GGGTTACTTC TACTTCACAT AGCTGGGCTC AGAGAATAGA CACGACAACA 
TCTGAGGAGG TAACTTCCAG TGAAACGAAG CAGGCCGTTC AGCCGCATCA GGACACGGTG 
AAGAAGGTTG TCCAGGAGAC CGTGATTGTG GAGGAGCGAC ACGGGATGAA CGTGCAGGCG 
AGCGGGGACC CTGCTAAAGC GGCTGGCCTT GCGCTGGATG CCCAGGCGGA GGCTGCTGGT 
GCCAAGGGCA AGGAGGGCTC TGCTGGGACT GAGGGGGAGA AGGAGGGAAA AAGGGAAGAG 
GCTGGGAAAG CCCCCACCAA ACTGGAAGGA GTCGCTGCTG CTTCGAGAGA GCAGGTGGAG 
GAGCACGCTA CAACAGGCCA CCTTGCAGAG TCTTTGGAAA GAAAATCTCA CTTTGAGTCG 
CCAGTTGTGA AAACTGAGAC TATCAGTTTC AGTAGCGTTT CTGCTGGTGG GGAAAACCTT 
GAAATTGCAA CAAAGGAAGT GCCAGTAGTC CATACAGAAA CAAAAACCAT TACATATGAG 
TCTTCTCAGG TGGATTGTGG TGCTGACTCA GAACCGGGTG TATTGATGAG TGCACAGACT 
ATCACATCTG AAACCACCAG CACTACAACC ACTACACATA TCACCAAAAC TGTGAAAGGA 
GGCATCTCAG AGACAAGAAT CGAAAAACGA ATAGTCATCA CAGGAGATGC AGATATTGAT 
CACGATCAGG CGCTGGCTCA GGCAATTAAA GAAGCCAAAG AGCAGCACCC TGACATGTCA 
GTGACCAAAG TAGTGGTACA TAAAGAGACA GAAATCACAC CAGAAGATGG GGAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794017.1
CDS	150..3809
Translation	MTTESGSDSESKDKEQDQQESPAQQGAAETQPQEQQEQQQQSEEHQNALEQFSAVAAHSTPVKKEQATDKEQEFAAASAKQLEYQQLEDDKLSQKSSSSKLSKSPLKIVKKPKNMQCKVTLLDGSEYACEVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDTENQKNWLDPAKEIKKQIRSGAWQFAFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDYDPDEYGSDYVSEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRASALIDRPAPYFERSSSKRYTMSRSLDGASVNENHEMYMKDSVSAAEVGTGQYATTKGISQTNLITTVTPEKKAEEEKDDEEGKKKRAEEVTPVSAIRHDTKNSLIKRIQGENVYVKHSNLMLEDLDKTQEDLMKHQTNISELKRTFLETSTETTVSNEWEKRLSTSPVRLAARQEEAPMIEPLVPEETKEEREKSEKLIFLQKGGTTFLELQPSVIEKKLQEGESAGTLVTSHQIIIQKSIPSSLEGTEDWVIIDKLPSEVVDGESKKNLAYKVVTVSSKTSFPPEILKSSTILMQSFEDLESEIQSKEENKQQMFTLGKSYDTVSGKIVTVASKAKEGEKTVQPSVEALQKMEREVQESVKIIPVVAEYEILEPVTDEKARRGSDVQSTKRKLSDSLTPIKEAESQLQSPEEESLKKTLKMDQDVQLHGTLGSLQLGKAEKHLGSEAVKAGAFARRDKSLSEWRYSREQPFTIATAHYVTESSASKVVTKQSTGEKTLDGSDIFSLIESARKPTEFIGGVTSTSHSWAQRIDTTTSEEVTSSETKQAVQPHQDTVKKVVQETVIVEERHGMNVQASGDPAKAAGLALDAQAEAAGAKGKEGSAGTEGEKEGKREEAGKAPTKLEGVAAASREQVEEHATTGHLAESLERKSHFESPVVKTETISFSSVSAGGENLEIATKEVPVVHTETKTITYESSQVDCGADSEPGVLMSAQTITSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEITPEDGED

Target ORF information:

RefSeq Version	XM_014938531.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014938531.1

ORF Insert Sequence:

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGAATAG TCTGATTAAG CGAATACAGG GTGAAAATGT GTATGTCAAA 
CATAGTAATC TTATGTTAGA GGACCTAGAC AAAACCCAGG AAGATCTGAT GAAACATCAG 
ACCAATATAA GCGAGCTGAA ACGCACCTTC TTGGAAACCT CCACAGAAAC AACTGTGTCT 
AATGAGTGGG AGAAGAGGCT TTCCACATCT CCCGTTCGGC TTGCAGCAAG GCAGGAGGAA 
GCTCCTATGA TTGAACCACT AGTGCCTGAA GAGACCAAAG AAGAAAGAGA AAAATCAGAA 
AAGCTCATAT TTTTGCAGAA GGGAGGTACT ACATTCCTTG AATTGCAGCC AAGTGTGATA 
GAGAAGAAGC TGCAGGAGGG AGAATCTGCT GGCACTTTGG TTACTTCTCA TCAAATCATT 
ATCCAGAAAA GTATCCCATC ATCCCTAGAG GGCACTGAGG ATTGGGTTAT TATAGACAAA 
TTACCCTCTG AGGTAGTTGA TGGTGAATCG AAAAAAAATC TGGCATACAA AGTAGTGACT 
GTGAGCAGTA AAACTTCCTT TCCACCTGAG ATATTAAAAT CCAGTACCAT TCTAATGCAG 
AGCTTTGAGG ACTTGGAAAG TGAAATACAA TCCAAAGAGG AGAATAAGCA GCAAATGTTC 
ACCCTAGGAA AGTCCTATGA TACTGTATCC GGGAAAATTG TTACCGTGGC AAGTAAAGCT 
AAAGAGGGTG AGAAAACAGT ACAGCCTTCA GTAGAAGCTT TGCAAAAAAT GGAAAGGGAA 
GTGCAAGAAT CTGTGAAAAT CATTCCGGTA GTGGCTGAGT ATGAAATCCT CGAGCCTGTC 
ACTGATGAGA AAGCCAGGCG GGGATCCGAC GTGCAGAGCA CGAAAAGAAA GCTGTCCGAC 
TCCCTGACAC CCATAAAGGA AGCAGAATCT CAGTTGCAAA GTCCTGAAGA GGAGAGTTTG 
AAAAAGACCC TAAAGATGGA CCAGGATGTG CAGTTACATG GTACACTGGG AAGCTTGCAG 
CTTGGCAAAG CAGAAAAACA CCTTGGCAGT GAAGCTGTAA AAGCAGGGGC GTTTGCCCGG 
AGAGATAAGA GCCTGTCTGA GTGGAGATAT TCCAGAGAAC AGCCATTTAC CATTGCTACT 
GCTCACTATG TTACCGAGTC GTCTGCATCA AAAGTAGTGA CTAAGCAGTC CACCGGTGAA 
AAAACCTTGG ATGGTTCAGA CATCTTCAGT TTAATAGAGT CCGCCAGAAA ACCGACTGAG 
TTCATAGGAG GGGTTACTTC TACTTCACAT AGCTGGGCTC AGAGAATAGA CACGACAACA 
TCTGAGGAGG TAACTTCCAG TGAAACGAAG CAGGCCGTTC AGCCGCATCA GGACACGGTG 
AAGAAGGTTG TCCAGGAGAC CGTGATTGTG GAGGAGCGAC ACGGGATGAA CGTGCAGGCG 
AGCGGGGACC CTGCTAAAGC GGCTGGCCTT GCGCTGGATG CCCAGGCGGA GGCTGCTGGT 
GCCAAGGGCA AGGAGGGCTC TGCTGGGACT GAGGGGGAGA AGGAGGGAAA AAGGGAAGAG 
GCTGGGAAAG CCCCCACCAA ACTGGAAGGA GTCGCTGCTG CTTCGAGAGA GCAGGTGGAG 
GAGCACGCTA CAACAGGCCA CCTTGCAGAG TCTTTGGAAA GAAAATCTCA CTTTGAGTCG 
CCAGTTGTGA AAACTGAGAC TATCAGTTTC AGTAGCGTTT CTGCTGGTGG GGAAAACCTT 
GAAATTGCAA CAAAGGAAGT GCCAGTAGTC CATACAGAAA CAAAAACCAT TACATATGAG 
TCTTCTCAGG TGGATTGTGG TGCTGACTCA GAACCGGGTG TATTGATGAG TGCACAGACT 
ATCACATCTG AAACCACCAG CACTACAACC ACTACACATA TCACCAAAAC TGTGAAAGGA 
GGCATCTCAG AGACAAGAAT CGAAAAACGA ATAGTCATCA CAGGAGATGC AGATATTGAT 
CACGATCAGG CGCTGGCTCA GGCAATTAAA GAAGCCAAAG AGCAGCACCC TGACATGTCA 
GTGACCAAAG TAGTGGTACA TAAAGAGACA GAAATCACAC CAGAAGATGG GGAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPB41L3 ( XM_014938532.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794018.1 Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X5, mRNA.

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CloneID	OCa62521
Clone ID Related Accession (Same CDS sequence)	XM_014938532.1
Accession Version	XM_014938532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	band 4.1-like protein 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGTCGGA TCAAGACGAG GACGGAGATC TAAAGGCACA GGACCTAGAC 
AAAACCCAGG AAGATCTGAT GAAACATCAG ACCAATATAA GCGAGCTGAA ACGCACCTTC 
TTGGAAACCT CCACAGAAAC AACTGTGTCT AATGAGTGGG AGAAGAGGCT TTCCACATCT 
CCCGTTCGGC TTGCAGCAAG GCAGGAGGAA GCTCCTATGA TTGAACCACT AGTGCCTGAA 
GAGACCAAAG AAGAAAGAGA AAAATCAGAA AAGCTCATAT TTTTGCAGAA GGGAGGTACT 
ACATTCCTTG AATTGCAGCC AAGTGTGATA GAGAAGAAGC TGCAGGAGGG AGAATCTGCT 
GGCACTTTGG TTACTTCTCA TCAAATCATT ATCCAGAAAA GTATCCCATC ATCCCTAGAG 
GGCACTGAGG ATTGGGTTAT TATAGACAAA TTACCCTCTG AGGTAGTTGA TGGTGAATCG 
AAAAAAAATC TGGCATACAA AGTAGTGACT GTGAGCAGTA AAACTTCCTT TCCACCTGAG 
ATATTAAAAT CCAGTACCAT TCTAATGCAG AGCTTTGAGG ACTTGGAAAG TGAAATACAA 
TCCAAAGAGG AGAATAAGCA GCAAATGTTC ACCCTAGGAA AGTCCTATGA TACTGTATCC 
GGGAAAATTG TTACCGTGGC AAGTAAAGCT AAAGAGGGTG AGAAAACAGT ACAGCCTTCA 
GTAGAAGCTT TGCAAAAAAT GGAAAGGGAA GTGCAAGAAT CTGTGAAAAT CATTCCGGTA 
GTGGCTGAGT ATGAAATCCT CGAGCCTGTC ACTGATGAGA AAGCCAGGCG GGGATCCGAC 
GTGCAGAGCA CGAAAAGAAA GCTGTCCGAC TCCCTGACAC CCATAAAGGA AGCAGAATCT 
CAGTTGCAAA GTCCTGAAGA GGAGAGTTTG AAAAAGACCC TAAAGATGGA CCAGGATGTG 
CAGTTACATG GTACACTGGG AAGCTTGCAG CTTGGCAAAG CAGAAAAACA CCTTGGCAGT 
GAAGCTGTAA AAGCAGGGGC GTTTGCCCGG AGAGATAAGA GCCTGTCTGA GTGGAGATAT 
TCCAGAGAAC AGCCATTTAC CATTGCTACT GCTCACTATG TTACCGAGTC GTCTGCATCA 
AAAGTAGTGA CTAAGCAGTC CACCGGTGAA AAAACCTTGG ATGGTTCAGA CATCTTCAGT 
TTAATAGAGT CCGCCAGAAA ACCGACTGAG TTCATAGGAG GGGTTACTTC TACTTCACAT 
AGCTGGGCTC AGAGAATAGA CACGACAACA TCTGAGGAGG TAACTTCCAG TGAAACGAAG 
CAGGCCGTTC AGCCGCATCA GGACACGGTG AAGAAGGTTG TCCAGGAGAC CGTGATTGTG 
GAGGAGCGAC ACGGGATGAA CGTGCAGGCG AGCGGGGACC CTGCTAAAGC GGCTGGCCTT 
GCGCTGGATG CCCAGGCGGA GGCTGCTGGT GCCAAGGGCA AGGAGGGCTC TGCTGGGACT 
GAGGGGGAGA AGGAGGGAAA AAGGGAAGAG GCTGGGAAAG CCCCCACCAA ACTGGAAGGA 
GTCGCTGCTG CTTCGAGAGA GCAGGTGGAG GAGCACGCTA CAACAGGCCA CCTTGCAGAG 
TCTTTGGAAA GAAAATCTCA CTTTGAGTCG CCAGTTGTGA AAACTGAGAC TATCAGTTTC 
AGTAGCGTTT CTGCTGGTGG GGAAAACCTT GAAATTGCAA CAAAGGAAGT GCCAGTAGTC 
CATACAGAAA CAAAAACCAT TACATATGAG TCTTCTCAGG TGGATTGTGG TGCTGACTCA 
GAACCGGGTG TATTGATGAG TGCACAGACT ATCACATCTG AAACCACCAG CACTACAACC 
ACTACACATA TCACCAAAAC TGTGAAAGGA GGCATCTCAG AGACAAGAAT CGAAAAACGA 
ATAGTCATCA CAGGAGATGC AGATATTGAT CACGATCAGG CGCTGGCTCA GGCAATTAAA 
GAAGCCAAAG AGCAGCACCC TGACATGTCA GTGACCAAAG TAGTGGTACA TAAAGAGACA 
GAAATCACAC CAGAAGATGG GGAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794018.1
CDS	150..3779
Translation	MTTESGSDSESKDKEQDQQESPAQQGAAETQPQEQQEQQQQSEEHQNALEQFSAVAAHSTPVKKEQATDKEQEFAAASAKQLEYQQLEDDKLSQKSSSSKLSKSPLKIVKKPKNMQCKVTLLDGSEYACEVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDTENQKNWLDPAKEIKKQIRSGAWQFAFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDYDPDEYGSDYVSEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRASALIDRPAPYFERSSSKRYTMSRSLDGASVNENHEMYMKDSVSAAEVGTGQYATTKGISQTNLITTVTPEKKAEEEKDDEEGKKKRAEEVTPVSAIRHDTKSDQDEDGDLKAQDLDKTQEDLMKHQTNISELKRTFLETSTETTVSNEWEKRLSTSPVRLAARQEEAPMIEPLVPEETKEEREKSEKLIFLQKGGTTFLELQPSVIEKKLQEGESAGTLVTSHQIIIQKSIPSSLEGTEDWVIIDKLPSEVVDGESKKNLAYKVVTVSSKTSFPPEILKSSTILMQSFEDLESEIQSKEENKQQMFTLGKSYDTVSGKIVTVASKAKEGEKTVQPSVEALQKMEREVQESVKIIPVVAEYEILEPVTDEKARRGSDVQSTKRKLSDSLTPIKEAESQLQSPEEESLKKTLKMDQDVQLHGTLGSLQLGKAEKHLGSEAVKAGAFARRDKSLSEWRYSREQPFTIATAHYVTESSASKVVTKQSTGEKTLDGSDIFSLIESARKPTEFIGGVTSTSHSWAQRIDTTTSEEVTSSETKQAVQPHQDTVKKVVQETVIVEERHGMNVQASGDPAKAAGLALDAQAEAAGAKGKEGSAGTEGEKEGKREEAGKAPTKLEGVAAASREQVEEHATTGHLAESLERKSHFESPVVKTETISFSSVSAGGENLEIATKEVPVVHTETKTITYESSQVDCGADSEPGVLMSAQTITSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEITPEDGED

Target ORF information:

RefSeq Version	XM_014938532.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014938532.1

ORF Insert Sequence:

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGTCGGA TCAAGACGAG GACGGAGATC TAAAGGCACA GGACCTAGAC 
AAAACCCAGG AAGATCTGAT GAAACATCAG ACCAATATAA GCGAGCTGAA ACGCACCTTC 
TTGGAAACCT CCACAGAAAC AACTGTGTCT AATGAGTGGG AGAAGAGGCT TTCCACATCT 
CCCGTTCGGC TTGCAGCAAG GCAGGAGGAA GCTCCTATGA TTGAACCACT AGTGCCTGAA 
GAGACCAAAG AAGAAAGAGA AAAATCAGAA AAGCTCATAT TTTTGCAGAA GGGAGGTACT 
ACATTCCTTG AATTGCAGCC AAGTGTGATA GAGAAGAAGC TGCAGGAGGG AGAATCTGCT 
GGCACTTTGG TTACTTCTCA TCAAATCATT ATCCAGAAAA GTATCCCATC ATCCCTAGAG 
GGCACTGAGG ATTGGGTTAT TATAGACAAA TTACCCTCTG AGGTAGTTGA TGGTGAATCG 
AAAAAAAATC TGGCATACAA AGTAGTGACT GTGAGCAGTA AAACTTCCTT TCCACCTGAG 
ATATTAAAAT CCAGTACCAT TCTAATGCAG AGCTTTGAGG ACTTGGAAAG TGAAATACAA 
TCCAAAGAGG AGAATAAGCA GCAAATGTTC ACCCTAGGAA AGTCCTATGA TACTGTATCC 
GGGAAAATTG TTACCGTGGC AAGTAAAGCT AAAGAGGGTG AGAAAACAGT ACAGCCTTCA 
GTAGAAGCTT TGCAAAAAAT GGAAAGGGAA GTGCAAGAAT CTGTGAAAAT CATTCCGGTA 
GTGGCTGAGT ATGAAATCCT CGAGCCTGTC ACTGATGAGA AAGCCAGGCG GGGATCCGAC 
GTGCAGAGCA CGAAAAGAAA GCTGTCCGAC TCCCTGACAC CCATAAAGGA AGCAGAATCT 
CAGTTGCAAA GTCCTGAAGA GGAGAGTTTG AAAAAGACCC TAAAGATGGA CCAGGATGTG 
CAGTTACATG GTACACTGGG AAGCTTGCAG CTTGGCAAAG CAGAAAAACA CCTTGGCAGT 
GAAGCTGTAA AAGCAGGGGC GTTTGCCCGG AGAGATAAGA GCCTGTCTGA GTGGAGATAT 
TCCAGAGAAC AGCCATTTAC CATTGCTACT GCTCACTATG TTACCGAGTC GTCTGCATCA 
AAAGTAGTGA CTAAGCAGTC CACCGGTGAA AAAACCTTGG ATGGTTCAGA CATCTTCAGT 
TTAATAGAGT CCGCCAGAAA ACCGACTGAG TTCATAGGAG GGGTTACTTC TACTTCACAT 
AGCTGGGCTC AGAGAATAGA CACGACAACA TCTGAGGAGG TAACTTCCAG TGAAACGAAG 
CAGGCCGTTC AGCCGCATCA GGACACGGTG AAGAAGGTTG TCCAGGAGAC CGTGATTGTG 
GAGGAGCGAC ACGGGATGAA CGTGCAGGCG AGCGGGGACC CTGCTAAAGC GGCTGGCCTT 
GCGCTGGATG CCCAGGCGGA GGCTGCTGGT GCCAAGGGCA AGGAGGGCTC TGCTGGGACT 
GAGGGGGAGA AGGAGGGAAA AAGGGAAGAG GCTGGGAAAG CCCCCACCAA ACTGGAAGGA 
GTCGCTGCTG CTTCGAGAGA GCAGGTGGAG GAGCACGCTA CAACAGGCCA CCTTGCAGAG 
TCTTTGGAAA GAAAATCTCA CTTTGAGTCG CCAGTTGTGA AAACTGAGAC TATCAGTTTC 
AGTAGCGTTT CTGCTGGTGG GGAAAACCTT GAAATTGCAA CAAAGGAAGT GCCAGTAGTC 
CATACAGAAA CAAAAACCAT TACATATGAG TCTTCTCAGG TGGATTGTGG TGCTGACTCA 
GAACCGGGTG TATTGATGAG TGCACAGACT ATCACATCTG AAACCACCAG CACTACAACC 
ACTACACATA TCACCAAAAC TGTGAAAGGA GGCATCTCAG AGACAAGAAT CGAAAAACGA 
ATAGTCATCA CAGGAGATGC AGATATTGAT CACGATCAGG CGCTGGCTCA GGCAATTAAA 
GAAGCCAAAG AGCAGCACCC TGACATGTCA GTGACCAAAG TAGTGGTACA TAAAGAGACA 
GAAATCACAC CAGAAGATGG GGAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPB41L3 ( XM_014938533.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794019.1 Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X6, mRNA.

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CloneID	OCa62522
Clone ID Related Accession (Same CDS sequence)	XM_014938533.1
Accession Version	XM_014938533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	band 4.1-like protein 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGGACCT AGACAAAACC CAGGAAGATC TGATGAAACA TCAGACCAAT 
ATAAGCGAGC TGAAACGCAC CTTCTTGGAA ACCTCCACAG AAACAACTGT GTCTAATGAG 
TGGGAGAAGA GGCTTTCCAC ATCTCCCGTT CGGCTTGCAG CAAGGCAGGA GGAAGCTCCT 
ATGATTGAAC CACTAGTGCC TGAAGAGACC AAAGAAGAAA GAGAAAAATC AGAAAAGCTC 
ATATTTTTGC AGAAGGGAGG TACTACATTC CTTGAATTGC AGCCAAGTGT GATAGAGAAG 
AAGCTGCAGG AGGGAGAATC TGCTGGCACT TTGGTTACTT CTCATCAAAT CATTATCCAG 
AAAAGTATCC CATCATCCCT AGAGGGCACT GAGGATTGGG TTATTATAGA CAAATTACCC 
TCTGAGGTAG TTGATGGTGA ATCGAAAAAA AATCTGGCAT ACAAAGTAGT GACTGTGAGC 
AGTAAAACTT CCTTTCCACC TGAGATATTA AAATCCAGTA CCATTCTAAT GCAGAGCTTT 
GAGGACTTGG AAAGTGAAAT ACAATCCAAA GAGGAGAATA AGCAGCAAAT GTTCACCCTA 
GGAAAGTCCT ATGATACTGT ATCCGGGAAA ATTGTTACCG TGGCAAGTAA AGCTAAAGAG 
GGTGAGAAAA CAGTACAGCC TTCAGTAGAA GCTTTGCAAA AAATGGAAAG GGAAGTGCAA 
GAATCTGTGA AAATCATTCC GGTAGTGGCT GAGTATGAAA TCCTCGAGCC TGTCACTGAT 
GAGAAAGCCA GGCGGGGATC CGACGTGCAG AGCACGAAAA GAAAGCTGTC CGACTCCCTG 
ACACCCATAA AGGAAGCAGA ATCTCAGTTG CAAAGTCCTG AAGAGGAGAG TTTGAAAAAG 
ACCCTAAAGA TGGACCAGGA TGTGCAGTTA CATGGTACAC TGGGAAGCTT GCAGCTTGGC 
AAAGCAGAAA AACACCTTGG CAGTGAAGCT GTAAAAGCAG GGGCGTTTGC CCGGAGAGAT 
AAGAGCCTGT CTGAGTGGAG ATATTCCAGA GAACAGCCAT TTACCATTGC TACTGCTCAC 
TATGTTACCG AGTCGTCTGC ATCAAAAGTA GTGACTAAGC AGTCCACCGG TGAAAAAACC 
TTGGATGGTT CAGACATCTT CAGTTTAATA GAGTCCGCCA GAAAACCGAC TGAGTTCATA 
GGAGGGGTTA CTTCTACTTC ACATAGCTGG GCTCAGAGAA TAGACACGAC AACATCTGAG 
GAGGTAACTT CCAGTGAAAC GAAGCAGGCC GTTCAGCCGC ATCAGGACAC GGTGAAGAAG 
GTTGTCCAGG AGACCGTGAT TGTGGAGGAG CGACACGGGA TGAACGTGCA GGCGAGCGGG 
GACCCTGCTA AAGCGGCTGG CCTTGCGCTG GATGCCCAGG CGGAGGCTGC TGGTGCCAAG 
GGCAAGGAGG GCTCTGCTGG GACTGAGGGG GAGAAGGAGG GAAAAAGGGA AGAGGCTGGG 
AAAGCCCCCA CCAAACTGGA AGGAGTCGCT GCTGCTTCGA GAGAGCAGGT GGAGGAGCAC 
GCTACAACAG GCCACCTTGC AGAGTCTTTG GAAAGAAAAT CTCACTTTGA GTCGCCAGTT 
GTGAAAACTG AGACTATCAG TTTCAGTAGC GTTTCTGCTG GTGGGGAAAA CCTTGAAATT 
GCAACAAAGG AAGTGCCAGT AGTCCATACA GAAACAAAAA CCATTACATA TGAGTCTTCT 
CAGGTGGATT GTGGTGCTGA CTCAGAACCG GGTGTATTGA TGAGTGCACA GACTATCACA 
TCTGAAACCA CCAGCACTAC AACCACTACA CATATCACCA AAACTGTGAA AGGAGGCATC 
TCAGAGACAA GAATCGAAAA ACGAATAGTC ATCACAGGAG ATGCAGATAT TGATCACGAT 
CAGGCGCTGG CTCAGGCAAT TAAAGAAGCC AAAGAGCAGC ACCCTGACAT GTCAGTGACC 
AAAGTAGTGG TACATAAAGA GACAGAAATC ACACCAGAAG ATGGGGAGGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794019.1
CDS	150..3743
Translation	MTTESGSDSESKDKEQDQQESPAQQGAAETQPQEQQEQQQQSEEHQNALEQFSAVAAHSTPVKKEQATDKEQEFAAASAKQLEYQQLEDDKLSQKSSSSKLSKSPLKIVKKPKNMQCKVTLLDGSEYACEVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDTENQKNWLDPAKEIKKQIRSGAWQFAFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDYDPDEYGSDYVSEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRASALIDRPAPYFERSSSKRYTMSRSLDGASVNENHEMYMKDSVSAAEVGTGQYATTKGISQTNLITTVTPEKKAEEEKDDEEGKKKRAEEVTPVSAIRHDTKDLDKTQEDLMKHQTNISELKRTFLETSTETTVSNEWEKRLSTSPVRLAARQEEAPMIEPLVPEETKEEREKSEKLIFLQKGGTTFLELQPSVIEKKLQEGESAGTLVTSHQIIIQKSIPSSLEGTEDWVIIDKLPSEVVDGESKKNLAYKVVTVSSKTSFPPEILKSSTILMQSFEDLESEIQSKEENKQQMFTLGKSYDTVSGKIVTVASKAKEGEKTVQPSVEALQKMEREVQESVKIIPVVAEYEILEPVTDEKARRGSDVQSTKRKLSDSLTPIKEAESQLQSPEEESLKKTLKMDQDVQLHGTLGSLQLGKAEKHLGSEAVKAGAFARRDKSLSEWRYSREQPFTIATAHYVTESSASKVVTKQSTGEKTLDGSDIFSLIESARKPTEFIGGVTSTSHSWAQRIDTTTSEEVTSSETKQAVQPHQDTVKKVVQETVIVEERHGMNVQASGDPAKAAGLALDAQAEAAGAKGKEGSAGTEGEKEGKREEAGKAPTKLEGVAAASREQVEEHATTGHLAESLERKSHFESPVVKTETISFSSVSAGGENLEIATKEVPVVHTETKTITYESSQVDCGADSEPGVLMSAQTITSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEITPEDGED

Target ORF information:

RefSeq Version	XM_014938533.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax erythrocyte membrane protein band 4.1-like 3 (EPB41L3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014938533.1

ORF Insert Sequence:

ATGACTACAG AATCGGGATC GGACTCCGAG TCCAAGGATA AAGAGCAGGA CCAGCAGGAG 
AGCCCCGCGC AGCAAGGGGC GGCCGAGACC CAGCCGCAGG AGCAGCAGGA GCAGCAGCAG 
CAGAGCGAGG AGCATCAGAA CGCGCTCGAG CAGTTCTCAG CGGTGGCAGC CCACAGCACG 
CCGGTGAAAA AGGAGCAGGC CACAGACAAA GAACAGGAGT TTGCTGCTGC GAGTGCAAAA 
CAGCTGGAAT ACCAGCAGCT AGAGGACGAC AAGCTCTCTC AAAAATCATC GTCTAGCAAG 
CTTTCCAAGT CTCCTCTGAA GATCGTCAAG AAACCGAAAA ATATGCAGTG CAAAGTGACA 
CTCCTTGATG GATCGGAATA TGCATGTGAA GTAGAGAAAC GCTCCAGAGG TCAAGTGCTA 
TTTGACAAAG TGTGCGAGCA TTTGAACCTT TTGGAAAAAG ACTACTTTGG GCTAACCTAC 
AGAGACACGG AAAACCAAAA GAATTGGTTG GACCCTGCCA AGGAAATCAA GAAGCAGATC 
CGAAGTGGTG CTTGGCAGTT TGCATTTAAT GTGAAATTCT ACCCTCCGGA CCCTGCCCAG 
CTATCTGAAG ATATTACCAG GTACTACCTC TGCTTGCAGC TGAGAGATGA CATCGTGTCA 
GGTCGGCTGC CGTGCTCATT CGTCACCCTG GCCCTGCTGG GCTCCTACAC CGTGCAGTCG 
GAGCTCGGCG ACTACGACCC CGACGAGTAC GGCAGCGACT ACGTCAGCGA GTTTCGCTTC 
GCGCCGAATC ACACGAAAGA GCTGGAGGAT AAAGTGATTG AGCTGCACAA GAGCCACAGG 
GGAATGACAC CTGCAGAGGC TGAGATGCAT TTCCTGGAGA ATGCCAAAAA ACTGTCAATG 
TATGGAGTAG ATCTCCATCA TGCCAAGGAT TCTGAAGGAG TGGAAATCAT GCTAGGAGTT 
TGTGCAAGTG GTCTCTTAAT ATATCGAGAC AGACTCAGAA TAAATAGATT TGCCTGGCCA 
AAGGTTCTGA AAATTTCCTA CAAGAGGAAC AACTTCTACA TCAAAATCCG ACCAGGGGAG 
TTTGAGCAGT TTGAAAGTAC TATTGGGTTT AAGTTGCCAA ATCATCGTGC TGCAAAGCGC 
TTATGGAAAG TGTGTGTTGA GCACCATACA TTTTTCAGGC TCCTGCTGCC AGAAGCACCT 
CCAAAGAAGT TCCTCACGCT GGGCTCCAAG TTTCGCTACA GCGGCCGGAC GCAGGCCCAG 
ACGAGAAGAG CCAGTGCCCT CATCGACCGT CCCGCACCTT ACTTCGAGCG GTCTTCTAGT 
AAACGTTACA CTATGTCCCG CAGCTTAGAT GGGGCATCAG TGAATGAAAA CCATGAAATG 
TACATGAAGG ATTCTGTGTC TGCTGCAGAG GTTGGTACTG GCCAGTACGC CACAACAAAG 
GGCATCTCTC AGACCAACTT GATAACCACT GTGACTCCGG AGAAAAAGGC AGAAGAAGAG 
AAAGATGATG AAGAGGGCAA AAAGAAAAGA GCAGAGGAAG TCACCCCGGT CTCGGCAATA 
CGCCATGACA CCAAGGACCT AGACAAAACC CAGGAAGATC TGATGAAACA TCAGACCAAT 
ATAAGCGAGC TGAAACGCAC CTTCTTGGAA ACCTCCACAG AAACAACTGT GTCTAATGAG 
TGGGAGAAGA GGCTTTCCAC ATCTCCCGTT CGGCTTGCAG CAAGGCAGGA GGAAGCTCCT 
ATGATTGAAC CACTAGTGCC TGAAGAGACC AAAGAAGAAA GAGAAAAATC AGAAAAGCTC 
ATATTTTTGC AGAAGGGAGG TACTACATTC CTTGAATTGC AGCCAAGTGT GATAGAGAAG 
AAGCTGCAGG AGGGAGAATC TGCTGGCACT TTGGTTACTT CTCATCAAAT CATTATCCAG 
AAAAGTATCC CATCATCCCT AGAGGGCACT GAGGATTGGG TTATTATAGA CAAATTACCC 
TCTGAGGTAG TTGATGGTGA ATCGAAAAAA AATCTGGCAT ACAAAGTAGT GACTGTGAGC 
AGTAAAACTT CCTTTCCACC TGAGATATTA AAATCCAGTA CCATTCTAAT GCAGAGCTTT 
GAGGACTTGG AAAGTGAAAT ACAATCCAAA GAGGAGAATA AGCAGCAAAT GTTCACCCTA 
GGAAAGTCCT ATGATACTGT ATCCGGGAAA ATTGTTACCG TGGCAAGTAA AGCTAAAGAG 
GGTGAGAAAA CAGTACAGCC TTCAGTAGAA GCTTTGCAAA AAATGGAAAG GGAAGTGCAA 
GAATCTGTGA AAATCATTCC GGTAGTGGCT GAGTATGAAA TCCTCGAGCC TGTCACTGAT 
GAGAAAGCCA GGCGGGGATC CGACGTGCAG AGCACGAAAA GAAAGCTGTC CGACTCCCTG 
ACACCCATAA AGGAAGCAGA ATCTCAGTTG CAAAGTCCTG AAGAGGAGAG TTTGAAAAAG 
ACCCTAAAGA TGGACCAGGA TGTGCAGTTA CATGGTACAC TGGGAAGCTT GCAGCTTGGC 
AAAGCAGAAA AACACCTTGG CAGTGAAGCT GTAAAAGCAG GGGCGTTTGC CCGGAGAGAT 
AAGAGCCTGT CTGAGTGGAG ATATTCCAGA GAACAGCCAT TTACCATTGC TACTGCTCAC 
TATGTTACCG AGTCGTCTGC ATCAAAAGTA GTGACTAAGC AGTCCACCGG TGAAAAAACC 
TTGGATGGTT CAGACATCTT CAGTTTAATA GAGTCCGCCA GAAAACCGAC TGAGTTCATA 
GGAGGGGTTA CTTCTACTTC ACATAGCTGG GCTCAGAGAA TAGACACGAC AACATCTGAG 
GAGGTAACTT CCAGTGAAAC GAAGCAGGCC GTTCAGCCGC ATCAGGACAC GGTGAAGAAG 
GTTGTCCAGG AGACCGTGAT TGTGGAGGAG CGACACGGGA TGAACGTGCA GGCGAGCGGG 
GACCCTGCTA AAGCGGCTGG CCTTGCGCTG GATGCCCAGG CGGAGGCTGC TGGTGCCAAG 
GGCAAGGAGG GCTCTGCTGG GACTGAGGGG GAGAAGGAGG GAAAAAGGGA AGAGGCTGGG 
AAAGCCCCCA CCAAACTGGA AGGAGTCGCT GCTGCTTCGA GAGAGCAGGT GGAGGAGCAC 
GCTACAACAG GCCACCTTGC AGAGTCTTTG GAAAGAAAAT CTCACTTTGA GTCGCCAGTT 
GTGAAAACTG AGACTATCAG TTTCAGTAGC GTTTCTGCTG GTGGGGAAAA CCTTGAAATT 
GCAACAAAGG AAGTGCCAGT AGTCCATACA GAAACAAAAA CCATTACATA TGAGTCTTCT 
CAGGTGGATT GTGGTGCTGA CTCAGAACCG GGTGTATTGA TGAGTGCACA GACTATCACA 
TCTGAAACCA CCAGCACTAC AACCACTACA CATATCACCA AAACTGTGAA AGGAGGCATC 
TCAGAGACAA GAATCGAAAA ACGAATAGTC ATCACAGGAG ATGCAGATAT TGATCACGAT 
CAGGCGCTGG CTCAGGCAAT TAAAGAAGCC AAAGAGCAGC ACCCTGACAT GTCAGTGACC 
AAAGTAGTGG TACATAAAGA GACAGAAATC ACACCAGAAG ATGGGGAGGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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EPB41L3 cDNA ORF clone, Calidris pugnax(ruff)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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