LOC106886152 cDNA ORF clone, Calidris pugnax(ruff)

  • Gene

  • Clones

  • gRNAs

The following LOC106886152 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106886152 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa62762 XM_014938863.1
Latest version!
Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCa62758 XM_014938859.1
Latest version!
Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa62759 XM_014938860.1
Latest version!
Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa62760 XM_014938861.1
Latest version!
Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa62761 XM_014938862.1
Latest version!
Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa62762
    Clone ID Related Accession (Same CDS sequence) XM_014938863.1
    Accession Version XM_014938863.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product multiple epidermal growth factor-like domains protein 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090811.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACATAAATG AGTGCACCAC TGAGAACGGT
    GGCTGTCATG ACCAGTGCTG TAATACCATC GGCAGCTACC ACTGCAAATG TCCAGCTGGC
    CAGAAACTGG AGGAAGATGG AAAATCCTGT GGAGACGTGG ACGAGTGCGC GGTGGTGAAC
    GGTGGCTGCC AGCAAGGCTG TGTCAACACA CACGGCTCCT TCCACTGCCA GTGCCGGGCG
    GGATACAGGC TCCACGCTGA TGGGCGCACC TGCATAGCCA TCAATTCCTG CAGTGTTAAC
    AAGGGGGGCT GCGAGCAGGA GTGCCTTCAG CTCGGTGAGG ACCGCTACAA GTGCCAGTGC
    CACCCGGGCT ACCAGCTGAA GAGGGATGCC AAATCCTGTG AAGACGTTGA TGAGTGTGCA
    GAAGGGAGAG CCAGGTGTGC TCATCGTTGT GTGAACACTC CAGGATCCTT CACCTGTGAC
    TGCAACCCCG GCTTCGAGCT GGGGGCTGAC GGGAAGCAGT GCTACCGCAT CGAAATGGAA
    ATTGTCGATA GCTGCCAGGA CAGCAATGGT GGCTGCTCTC ATCACTGCGA GCACACGACT
    GCGGGGCCGC GCTGCTCCTG CGACGACGGC TATCGCCTGG GTGTTGATGG CAAAACGTGT
    GCAGAGGTGG ACGAGTGTGA GACCGGAGAG TCCTGCTGCT CCCAATTCTG CATCAACTAC
    GTGGGAGGCT ACGAGTGTGC CTGCAAAGCG GGGTTCCAGC CCAACGCCGA CGGCTGCGCC
    TGCGACGATG TGGATGAATG TCGTCTTGAT AATGGCAACT GTGACCATTT CTGTGTTAAT
    TCTCTGGGGT CATACGAATG TGCGTGTAAG GAGGGTTACA GGCTTGGTGT TAACAGATGG
    TCCTGCATCG CCCTAGGTGG GGAGGAGACA GATGTGGAGG AGGCGACGGG GTTCGCCGGG
    GCTCCCGGGC TGCAGTTCAG AGGTCCCCCC CAGCTCCTTC ACTACGCCCT CGGTGCCCTC
    TACGAGGATG AAGACCCACG AGGAGAACTC ACCCTGGTGC ACAGGGTCGT TTGCCTCGAT
    AACACTTTTG GCAACGACTG TAGCTTGAGC TGTGAGGATT GTCTGAACGG AGGCAGATGT
    AACAGCGAAG GCAGCGGCTG CGTGTGCTCG CCCGGGTGGA GCGGCATTAT CTGTAATGAA
    ACTTGTTCCC CCGGGACGTA CGGCAGAGGC TGTGCCAGTG TTTGCAAGTG CCAGAACGGA
    GGCTCCTGTG ACCCCGTCAC CGGGCAGTGC CGCTGTCCCC CCGGCGTGCA CGGCAAGCTC
    TGTGAAGATG GTTGCCCAAA GGGTTTTTTT GGGAAGAACT GTAACAAACC ATGCAACTGC
    GCCAACAACG GCTATTGCCA CAGACTGTAC GGAGCCTGCC TGTGTGACCC CGGGCTCTAC
    GGGCGCTTCT GCCACCTCAC CTGTCCCAGG TGGGCCTTCG GAGCCGGCTG CTCGGAGGAG
    TGTCAGTGCG TGAAGGAGCA CACGCTGGAA TGCAATGCCA GGAACGGGAC TTGCACCTGC
    AAGCCAGGTT ACCACGGCAA AAAGTGTCAG AAAGAGTGCA TGCCTGGCTT TTATGGGGCT
    GGTTGCAAAC TGAGGTGCAA CTGTCCTCCT GATGTGTCGT GTAACCGTGA GACGGGAGCC
    TGCCAGCATC CGTGTCCACC TGGGTTTCAT GGAGAAGAAT GCCATTTATC TTGTCAGCCC
    GGGAGCTTTG GGGTGAACTG CGGGCAGAGG TGCAGCTGTG GAGAAGCACC GTGTGATCCA
    AAGACGGGGC AGTGCATTTG CCCAGCTGGG AAGGCTGGTG CTACATGTGA GCAAGATTGC
    CCAGCGGGTC GGTGGGGACC AGACTGCCAG TCCTCCTGTG AGCCCTGTGC CAACGGGGGC
    CAGTGCAACA GAGAAACTGG AGCCTGCGAC TGTCCCCCGG GCTACACCGG GGCATCCTGC
    TCTGCCTCCT GCCCTGCTGG TTACTACGGA CAAAACTGCC TGCTGTCGTG CAGCTGTAGC
    AGTGGTGCTC AGTGTCACCA TGTCACCGGA GAGTGCACGT GTCCCCCAGG CTGGACCGGC
    CCCGACTGCA AACACCCCTG CAACAGTGGC CGTTGGGGCC TGCGCTGTGA AAACACGTGT
    GTCTGCAATA ACAGCGACGG TAGCTGCGAT CCCGTCACCG GCTCCTGCTT CTGTGAGCCA
    GGGTTCACTG GGGAACACTG CGAATGGAGG TGCCCCGAGG GAAGCTTTGG CCCGAGCTGT
    GAGTCCAGCT GCCAGTGCCA GAATGGAGCT GCCTGCGACC ACGTCAGCGG AGCCTGTACC
    TGCACGGCAG GCTGGACAGG AACCTTTTGT GAAAGAGCAT GTCCAGATGG ATTCTTTGGG
    CTTGACTGCC ACCAGGTGTG CGAGTGCAAG AATGCTGCTG GCTGCAACCA CGTCCTGGGA
    ACCTGCCGCT GCCTCCCGGG CTGGCGGGGA GAGACCTGCC AGCAGCCCTG CCAGGGGAAC
    AGCTATGGGC CGGGCTGCAA CCACACTTGT CACTGTCACA ACGGAGCCCT CTGTCATCAT
    GTAACCGGGA CGTGCCTTTG TAGCCCAGGG TGGAAAGGAA CCACCTGTCA AGAAGCCTGT
    CCTCCTGGAT GGTATGGTGC AAACTGTCTC CAGCGCTGCG TCTGCCACGG CCAGGCGACG
    TGTGACCGTG TGACGGGCGA GTGCCGGTGT CCCCAGGGTT GGATGGGGAT AGCCTGTGAG
    CTGGAGTGTG GGGACGGGAA GCACGGAGAG GGCTGTGGGC AGAACTGCAG CTGCCACCGT
    GGGGTTTGTG ACCAGCACTC AGGGAGGTGC ATCTGCCACG CCGGCTGGAC TGGAGACCGC
    TGCGATGTTG CCTGTCCCCC GGGATCTTTT GGCAAGCAGT GCGAGGAGCG GTGTGACTGC
    GTGCACGGCT TGTCCTGCCA CCATCAAACA GGAGCGTGTC ACTGTGCAAA GGGGTGGCGA
    GGGAGGCACT GTGACAAACC TTGCTTGCCT GGACGCTACG GTGTGGGCTG TGCCCAGCGG
    TGCCGGTGTC CTGCGGGTAC CCCCTGCCAC CACCTGACGG GCAAGTGCGG CTGTCCTCCG
    GGATTCACCG GCTACGGCTG CGAGAAAACT TGTCCACCGG GCACGTATGG TAAGAACTGT
    AACCGAGTGT GTCAGTGCTC TGCCGAGAAT GAGGAATGTC ACCCGGTGAC CGGCGACTGT
    GTGTGCCACC CGGGCTACCA CGGGGCCCGC TGCACCCTCC TGTGTCCCCA GGGACAATAC
    GGCCCCAACT GCCAGCTGAC ATGTACCTGT CAGAACGGTG GTATCTGTGA CCACGTGGAT
    GGCAACTGCA CTTGTGGGCT CGGCTGGACA GGAAGATCGT GTGAGAAAGC AGAATGTCTC
    CCAGGGAAGT ACGGGTCTGG CTGTGGCTTG GACTGTTCGT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCGGTGCCCC GAGGGCTACT ACGGCCACTC CTGCGAGCAC
    AGGTGTCCCC TTGGATTTTA TGGACTAAGC TGTCTTCATG CGTGTGCCTG TAAAAATGGA
    GGGAGCTGCG ATGCGGTGAC GGGACAGTGC ATGTGTCCTT TGGGTTATCA AGGTGTCCAC
    TGCGAGAAAG GCTGTGACAG GGGCTGGTTT GGAGAGAGGT GCCAGCATCA GTGCGACTGC
    GGAGATGCTC CATGTGATCC AGAGACTGGG AAGTGCCTTT GCCCACCTGG GAAGACCGGA
    GATAAATGTG ATGTTGAATG CAGGTCAGAC CAGTATGGCC CCAACTGCTC CTTACGATGC
    CAGTGTGCCA GCAAATCCCA GTGCAATCCA TACAACGGGA ACTGCGTCTG CCCGGCCACG
    TGGATGGGGC CAACCTGCAA AGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCAAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014794349.1
    CDS47..4471
    Translation

    Target ORF information:

    RefSeq Version XM_014938863.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014938863.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACATAAATG AGTGCACCAC TGAGAACGGT
    GGCTGTCATG ACCAGTGCTG TAATACCATC GGCAGCTACC ACTGCAAATG TCCAGCTGGC
    CAGAAACTGG AGGAAGATGG AAAATCCTGT GGAGACGTGG ACGAGTGCGC GGTGGTGAAC
    GGTGGCTGCC AGCAAGGCTG TGTCAACACA CACGGCTCCT TCCACTGCCA GTGCCGGGCG
    GGATACAGGC TCCACGCTGA TGGGCGCACC TGCATAGCCA TCAATTCCTG CAGTGTTAAC
    AAGGGGGGCT GCGAGCAGGA GTGCCTTCAG CTCGGTGAGG ACCGCTACAA GTGCCAGTGC
    CACCCGGGCT ACCAGCTGAA GAGGGATGCC AAATCCTGTG AAGACGTTGA TGAGTGTGCA
    GAAGGGAGAG CCAGGTGTGC TCATCGTTGT GTGAACACTC CAGGATCCTT CACCTGTGAC
    TGCAACCCCG GCTTCGAGCT GGGGGCTGAC GGGAAGCAGT GCTACCGCAT CGAAATGGAA
    ATTGTCGATA GCTGCCAGGA CAGCAATGGT GGCTGCTCTC ATCACTGCGA GCACACGACT
    GCGGGGCCGC GCTGCTCCTG CGACGACGGC TATCGCCTGG GTGTTGATGG CAAAACGTGT
    GCAGAGGTGG ACGAGTGTGA GACCGGAGAG TCCTGCTGCT CCCAATTCTG CATCAACTAC
    GTGGGAGGCT ACGAGTGTGC CTGCAAAGCG GGGTTCCAGC CCAACGCCGA CGGCTGCGCC
    TGCGACGATG TGGATGAATG TCGTCTTGAT AATGGCAACT GTGACCATTT CTGTGTTAAT
    TCTCTGGGGT CATACGAATG TGCGTGTAAG GAGGGTTACA GGCTTGGTGT TAACAGATGG
    TCCTGCATCG CCCTAGGTGG GGAGGAGACA GATGTGGAGG AGGCGACGGG GTTCGCCGGG
    GCTCCCGGGC TGCAGTTCAG AGGTCCCCCC CAGCTCCTTC ACTACGCCCT CGGTGCCCTC
    TACGAGGATG AAGACCCACG AGGAGAACTC ACCCTGGTGC ACAGGGTCGT TTGCCTCGAT
    AACACTTTTG GCAACGACTG TAGCTTGAGC TGTGAGGATT GTCTGAACGG AGGCAGATGT
    AACAGCGAAG GCAGCGGCTG CGTGTGCTCG CCCGGGTGGA GCGGCATTAT CTGTAATGAA
    ACTTGTTCCC CCGGGACGTA CGGCAGAGGC TGTGCCAGTG TTTGCAAGTG CCAGAACGGA
    GGCTCCTGTG ACCCCGTCAC CGGGCAGTGC CGCTGTCCCC CCGGCGTGCA CGGCAAGCTC
    TGTGAAGATG GTTGCCCAAA GGGTTTTTTT GGGAAGAACT GTAACAAACC ATGCAACTGC
    GCCAACAACG GCTATTGCCA CAGACTGTAC GGAGCCTGCC TGTGTGACCC CGGGCTCTAC
    GGGCGCTTCT GCCACCTCAC CTGTCCCAGG TGGGCCTTCG GAGCCGGCTG CTCGGAGGAG
    TGTCAGTGCG TGAAGGAGCA CACGCTGGAA TGCAATGCCA GGAACGGGAC TTGCACCTGC
    AAGCCAGGTT ACCACGGCAA AAAGTGTCAG AAAGAGTGCA TGCCTGGCTT TTATGGGGCT
    GGTTGCAAAC TGAGGTGCAA CTGTCCTCCT GATGTGTCGT GTAACCGTGA GACGGGAGCC
    TGCCAGCATC CGTGTCCACC TGGGTTTCAT GGAGAAGAAT GCCATTTATC TTGTCAGCCC
    GGGAGCTTTG GGGTGAACTG CGGGCAGAGG TGCAGCTGTG GAGAAGCACC GTGTGATCCA
    AAGACGGGGC AGTGCATTTG CCCAGCTGGG AAGGCTGGTG CTACATGTGA GCAAGATTGC
    CCAGCGGGTC GGTGGGGACC AGACTGCCAG TCCTCCTGTG AGCCCTGTGC CAACGGGGGC
    CAGTGCAACA GAGAAACTGG AGCCTGCGAC TGTCCCCCGG GCTACACCGG GGCATCCTGC
    TCTGCCTCCT GCCCTGCTGG TTACTACGGA CAAAACTGCC TGCTGTCGTG CAGCTGTAGC
    AGTGGTGCTC AGTGTCACCA TGTCACCGGA GAGTGCACGT GTCCCCCAGG CTGGACCGGC
    CCCGACTGCA AACACCCCTG CAACAGTGGC CGTTGGGGCC TGCGCTGTGA AAACACGTGT
    GTCTGCAATA ACAGCGACGG TAGCTGCGAT CCCGTCACCG GCTCCTGCTT CTGTGAGCCA
    GGGTTCACTG GGGAACACTG CGAATGGAGG TGCCCCGAGG GAAGCTTTGG CCCGAGCTGT
    GAGTCCAGCT GCCAGTGCCA GAATGGAGCT GCCTGCGACC ACGTCAGCGG AGCCTGTACC
    TGCACGGCAG GCTGGACAGG AACCTTTTGT GAAAGAGCAT GTCCAGATGG ATTCTTTGGG
    CTTGACTGCC ACCAGGTGTG CGAGTGCAAG AATGCTGCTG GCTGCAACCA CGTCCTGGGA
    ACCTGCCGCT GCCTCCCGGG CTGGCGGGGA GAGACCTGCC AGCAGCCCTG CCAGGGGAAC
    AGCTATGGGC CGGGCTGCAA CCACACTTGT CACTGTCACA ACGGAGCCCT CTGTCATCAT
    GTAACCGGGA CGTGCCTTTG TAGCCCAGGG TGGAAAGGAA CCACCTGTCA AGAAGCCTGT
    CCTCCTGGAT GGTATGGTGC AAACTGTCTC CAGCGCTGCG TCTGCCACGG CCAGGCGACG
    TGTGACCGTG TGACGGGCGA GTGCCGGTGT CCCCAGGGTT GGATGGGGAT AGCCTGTGAG
    CTGGAGTGTG GGGACGGGAA GCACGGAGAG GGCTGTGGGC AGAACTGCAG CTGCCACCGT
    GGGGTTTGTG ACCAGCACTC AGGGAGGTGC ATCTGCCACG CCGGCTGGAC TGGAGACCGC
    TGCGATGTTG CCTGTCCCCC GGGATCTTTT GGCAAGCAGT GCGAGGAGCG GTGTGACTGC
    GTGCACGGCT TGTCCTGCCA CCATCAAACA GGAGCGTGTC ACTGTGCAAA GGGGTGGCGA
    GGGAGGCACT GTGACAAACC TTGCTTGCCT GGACGCTACG GTGTGGGCTG TGCCCAGCGG
    TGCCGGTGTC CTGCGGGTAC CCCCTGCCAC CACCTGACGG GCAAGTGCGG CTGTCCTCCG
    GGATTCACCG GCTACGGCTG CGAGAAAACT TGTCCACCGG GCACGTATGG TAAGAACTGT
    AACCGAGTGT GTCAGTGCTC TGCCGAGAAT GAGGAATGTC ACCCGGTGAC CGGCGACTGT
    GTGTGCCACC CGGGCTACCA CGGGGCCCGC TGCACCCTCC TGTGTCCCCA GGGACAATAC
    GGCCCCAACT GCCAGCTGAC ATGTACCTGT CAGAACGGTG GTATCTGTGA CCACGTGGAT
    GGCAACTGCA CTTGTGGGCT CGGCTGGACA GGAAGATCGT GTGAGAAAGC AGAATGTCTC
    CCAGGGAAGT ACGGGTCTGG CTGTGGCTTG GACTGTTCGT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCGGTGCCCC GAGGGCTACT ACGGCCACTC CTGCGAGCAC
    AGGTGTCCCC TTGGATTTTA TGGACTAAGC TGTCTTCATG CGTGTGCCTG TAAAAATGGA
    GGGAGCTGCG ATGCGGTGAC GGGACAGTGC ATGTGTCCTT TGGGTTATCA AGGTGTCCAC
    TGCGAGAAAG GCTGTGACAG GGGCTGGTTT GGAGAGAGGT GCCAGCATCA GTGCGACTGC
    GGAGATGCTC CATGTGATCC AGAGACTGGG AAGTGCCTTT GCCCACCTGG GAAGACCGGA
    GATAAATGTG ATGTTGAATG CAGGTCAGAC CAGTATGGCC CCAACTGCTC CTTACGATGC
    CAGTGTGCCA GCAAATCCCA GTGCAATCCA TACAACGGGA ACTGCGTCTG CCCGGCCACG
    TGGATGGGGC CAACCTGCAA AGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCAAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa62758
    Clone ID Related Accession (Same CDS sequence) XM_014938859.1
    Accession Version XM_014938859.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090811.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACATAAATG AGTGCACCAC TGAGAACGGT
    GGCTGTCATG ACCAGTGCTG TAATACCATC GGCAGCTACC ACTGCAAATG TCCAGCTGGC
    CAGAAACTGG AGGAAGATGG AAAATCCTGT GGAGACGTGG ACGAGTGCGC GGTGGTGAAC
    GGTGGCTGCC AGCAAGGCTG TGTCAACACA CACGGCTCCT TCCACTGCCA GTGCCGGGCG
    GGATACAGGC TCCACGCTGA TGGGCGCACC TGCATAGCCA TCAATTCCTG CAGTGTTAAC
    AAGGGGGGCT GCGAGCAGGA GTGCCTTCAG CTCGGTGAGG ACCGCTACAA GTGCCAGTGC
    CACCCGGGCT ACCAGCTGAA GAGGGATGCC AAATCCTGTG AAGACGTTGA TGAGTGTGCA
    GAAGGGAGAG CCAGGTGTGC TCATCGTTGT GTGAACACTC CAGGATCCTT CACCTGTGAC
    TGCAACCCCG GCTTCGAGCT GGGGGCTGAC GGGAAGCAGT GCTACCGCAT CGAAATGGAA
    ATTGTCGATA GCTGCCAGGA CAGCAATGGT GGCTGCTCTC ATCACTGCGA GCACACGACT
    GCGGGGCCGC GCTGCTCCTG CGACGACGGC TATCGCCTGG GTGTTGATGG CAAAACGTGT
    GCAGAGGTGG ACGAGTGTGA GACCGGAGAG TCCTGCTGCT CCCAATTCTG CATCAACTAC
    GTGGGAGGCT ACGAGTGTGC CTGCAAAGCG GGGTTCCAGC CCAACGCCGA CGGCTGCGCC
    TGCGACGATG TGGATGAATG TCGTCTTGAT AATGGCAACT GTGACCATTT CTGTGTTAAT
    TCTCTGGGGT CATACGAATG TGCGTGTAAG GAGGGTTACA GGCTTGGTGT TAACAGATGG
    TCCTGCATCG CCCTAGGTGG GGAGGAGACA GATGTGGAGG AGGCGACGGG GTTCGCCGGG
    GCTCCCGGGC TGCAGTTCAG AGGTCCCCCC CAGCTCCTTC ACTACGCCCT CGGTGCCCTC
    TACGAGGATG AAGACCCACG AGGAGAACTC ACCCTGGTGC ACAGGGTCGT TTGCCTCGAT
    AACACTTTTG GCAACGACTG TAGCTTGAGC TGTGAGGATT GTCTGAACGG AGGCAGATGT
    AACAGCGAAG GCAGCGGCTG CGTGTGCTCG CCCGGGTGGA GCGGCATTAT CTGTAATGAA
    ACTTGTTCCC CCGGGACGTA CGGCAGAGGC TGTGCCAGTG TTTGCAAGTG CCAGAACGGA
    GGCTCCTGTG ACCCCGTCAC CGGGCAGTGC CGCTGTCCCC CCGGCGTGCA CGGCAAGCTC
    TGTGAAGATG GTTGCCCAAA GGGTTTTTTT GGGAAGAACT GTAACAAACC ATGCAACTGC
    GCCAACAACG GCTATTGCCA CAGACTGTAC GGAGCCTGCC TGTGTGACCC CGGGCTCTAC
    GGGCGCTTCT GCCACCTCAC CTGTCCCAGG TGGGCCTTCG GAGCCGGCTG CTCGGAGGAG
    TGTCAGTGCG TGAAGGAGCA CACGCTGGAA TGCAATGCCA GGAACGGGAC TTGCACCTGC
    AAGCCAGGTT ACCACGGCAA AAAGTGTCAG AAAGAGTGCA TGCCTGGCTT TTATGGGGCT
    GGTTGCAAAC TGAGGTGCAA CTGTCCTCCT GATGTGTCGT GTAACCGTGA GACGGGAGCC
    TGCCAGCATC CGTGTCCACC TGGGTTTCAT GGAGAAGAAT GCCATTTATC TTGTCAGCCC
    GGGAGCTTTG GGGTGAACTG CGGGCAGAGG TGCAGCTGTG GAGAAGCACC GTGTGATCCA
    AAGACGGGGC AGTGCATTTG CCCAGCTGGG AAGGCTGGTG CTACATGTGA GCAAGATTGC
    CCAGCGGGTC GGTGGGGACC AGACTGCCAG TCCTCCTGTG AGCCCTGTGC CAACGGGGGC
    CAGTGCAACA GAGAAACTGG AGCCTGCGAC TGTCCCCCGG GCTACACCGG GGCATCCTGC
    TCTGCCTCCT GCCCTGCTGG TTACTACGGA CAAAACTGCC TGCTGTCGTG CAGCTGTAGC
    AGTGGTGCTC AGTGTCACCA TGTCACCGGA GAGTGCACGT GTCCCCCAGG CTGGACCGGC
    CCCGACTGCA AACACCCCTG CAACAGTGGC CGTTGGGGCC TGCGCTGTGA AAACACGTGT
    GTCTGCAATA ACAGCGACGG TAGCTGCGAT CCCGTCACCG GCTCCTGCTT CTGTGAGCCA
    GGGTTCACTG GGGAACACTG CGAATGGAGG TGCCCCGAGG GAAGCTTTGG CCCGAGCTGT
    GAGTCCAGCT GCCAGTGCCA GAATGGAGCT GCCTGCGACC ACGTCAGCGG AGCCTGTACC
    TGCACGGCAG GCTGGACAGG AACCTTTTGT GAAAGAGCAT GTCCAGATGG ATTCTTTGGG
    CTTGACTGCC ACCAGGTGTG CGAGTGCAAG AATGCTGCTG GCTGCAACCA CGTCCTGGGA
    ACCTGCCGCT GCCTCCCGGG CTGGCGGGGA GAGACCTGCC AGCAGCCCTG CCAGGGGAAC
    AGCTATGGGC CGGGCTGCAA CCACACTTGT CACTGTCACA ACGGAGCCCT CTGTCATCAT
    GTAACCGGGA CGTGCCTTTG TAGCCCAGGG TGGAAAGGAA CCACCTGTCA AGAAGCCTGT
    CCTCCTGGAT GGTATGGTGC AAACTGTCTC CAGCGCTGCG TCTGCCACGG CCAGGCGACG
    TGTGACCGTG TGACGGGCGA GTGCCGGTGT CCCCAGGGTT GGATGGGGAT AGCCTGTGAG
    CTGGAGTGTG GGGACGGGAA GCACGGAGAG GGCTGTGGGC AGAACTGCAG CTGCCACCGT
    GGGGTTTGTG ACCAGCACTC AGGGAGGTGC ATCTGCCACG CCGGCTGGAC TGGAGACCGC
    TGCGATGTTG CCTGTCCCCC GGGATCTTTT GGCAAGCAGT GCGAGGAGCG GTGTGACTGC
    GTGCACGGCT TGTCCTGCCA CCATCAAACA GGAGCGTGTC ACTGTGCAAA GGGGTGGCGA
    GGGAGGCACT GTGACAAACC TTGCTTGCCT GGACGCTACG GTGTGGGCTG TGCCCAGCGG
    TGCCGGTGTC CTGCGGGTAC CCCCTGCCAC CACCTGACGG GCAAGTGCGG CTGTCCTCCG
    GGATTCACCG GCTACGGCTG CGAGAAAACT TGTCCACCGG GCACGTATGG TAAGAACTGT
    AACCGAGTGT GTCAGTGCTC TGCCGAGAAT GAGGAATGTC ACCCGGTGAC CGGCGACTGT
    GTGTGCCACC CGGGCTACCA CGGGGCCCGC TGCACCCTCC GGTGTCCAAA GGGTACTTAC
    GGTCCAAACT GCCAAAACCC CTGTAAATGC ATGAATGGAG GCCATTGCGA CCCCGTGTCG
    GGAACTTGTG ATTGCACGCC TGGTTTCATT GGAGCCGACT GTAGCAAAAC TTGCCCTGAA
    AACCGATACG GGAAGGACTG CACCCTGTTC TGTGCATGTG GTAAAGGTCA GTGTGACCCA
    CGGACTGGCA AGTGTTCCTG TCCTCCCGGC CAAATGGGAC CCAGCTGTCA GCAAGTGTGT
    CCCCAGGGAC AATACGGCCC CAACTGCCAG CTGACATGTA CCTGTCAGAA CGGTGGTATC
    TGTGACCACG TGGATGGCAA CTGCACTTGT GGGCTCGGCT GGACAGGAAG ATCGTGTGAG
    AAAGCAGAAT GTCTCCCAGG GAAGTACGGG TCTGGCTGTG GCTTGGACTG TTCGTGCCAG
    CACAACGGCA CCTGCGACAG GTTCACGGGC TGCTGCCGGT GCCCCGAGGG CTACTACGGC
    CACTCCTGCG AGCACAGGTG TCCCCTTGGA TTTTATGGAC TAAGCTGTCT TCATGCGTGT
    GCCTGTAAAA ATGGAGGGAG CTGCGATGCG GTGACGGGAC AGTGCATGTG TCCTTTGGGT
    TATCAAGGTG TCCACTGCGA GAAAGGCTGT GACAGGGGCT GGTTTGGAGA GAGGTGCCAG
    CATCAGTGCG ACTGCGGAGA TGCTCCATGT GATCCAGAGA CTGGGAAGTG CCTTTGCCCA
    CCTGGGAAGA CCGGAGATAA ATGTGATGTT GAATGCAGGT CAGACCAGTA TGGCCCCAAC
    TGCTCCTTAC GATGCCAGTG TGCCAGCAAA TCCCAGTGCA ATCCATACAA CGGGAACTGC
    GTCTGCCCGG CCACGTGGAT GGGGCCAACC TGCAAAGAAG GTGGCCCTCC AAAGCTTCCT
    TTTTATGAAG AACAAACTCA AGAAAGAGGG AATATTTTTC CAAGAGCTCT ACAACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014794345.1
    CDS47..4726
    Translation

    Target ORF information:

    RefSeq Version XM_014938859.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014938859.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACATAAATG AGTGCACCAC TGAGAACGGT
    GGCTGTCATG ACCAGTGCTG TAATACCATC GGCAGCTACC ACTGCAAATG TCCAGCTGGC
    CAGAAACTGG AGGAAGATGG AAAATCCTGT GGAGACGTGG ACGAGTGCGC GGTGGTGAAC
    GGTGGCTGCC AGCAAGGCTG TGTCAACACA CACGGCTCCT TCCACTGCCA GTGCCGGGCG
    GGATACAGGC TCCACGCTGA TGGGCGCACC TGCATAGCCA TCAATTCCTG CAGTGTTAAC
    AAGGGGGGCT GCGAGCAGGA GTGCCTTCAG CTCGGTGAGG ACCGCTACAA GTGCCAGTGC
    CACCCGGGCT ACCAGCTGAA GAGGGATGCC AAATCCTGTG AAGACGTTGA TGAGTGTGCA
    GAAGGGAGAG CCAGGTGTGC TCATCGTTGT GTGAACACTC CAGGATCCTT CACCTGTGAC
    TGCAACCCCG GCTTCGAGCT GGGGGCTGAC GGGAAGCAGT GCTACCGCAT CGAAATGGAA
    ATTGTCGATA GCTGCCAGGA CAGCAATGGT GGCTGCTCTC ATCACTGCGA GCACACGACT
    GCGGGGCCGC GCTGCTCCTG CGACGACGGC TATCGCCTGG GTGTTGATGG CAAAACGTGT
    GCAGAGGTGG ACGAGTGTGA GACCGGAGAG TCCTGCTGCT CCCAATTCTG CATCAACTAC
    GTGGGAGGCT ACGAGTGTGC CTGCAAAGCG GGGTTCCAGC CCAACGCCGA CGGCTGCGCC
    TGCGACGATG TGGATGAATG TCGTCTTGAT AATGGCAACT GTGACCATTT CTGTGTTAAT
    TCTCTGGGGT CATACGAATG TGCGTGTAAG GAGGGTTACA GGCTTGGTGT TAACAGATGG
    TCCTGCATCG CCCTAGGTGG GGAGGAGACA GATGTGGAGG AGGCGACGGG GTTCGCCGGG
    GCTCCCGGGC TGCAGTTCAG AGGTCCCCCC CAGCTCCTTC ACTACGCCCT CGGTGCCCTC
    TACGAGGATG AAGACCCACG AGGAGAACTC ACCCTGGTGC ACAGGGTCGT TTGCCTCGAT
    AACACTTTTG GCAACGACTG TAGCTTGAGC TGTGAGGATT GTCTGAACGG AGGCAGATGT
    AACAGCGAAG GCAGCGGCTG CGTGTGCTCG CCCGGGTGGA GCGGCATTAT CTGTAATGAA
    ACTTGTTCCC CCGGGACGTA CGGCAGAGGC TGTGCCAGTG TTTGCAAGTG CCAGAACGGA
    GGCTCCTGTG ACCCCGTCAC CGGGCAGTGC CGCTGTCCCC CCGGCGTGCA CGGCAAGCTC
    TGTGAAGATG GTTGCCCAAA GGGTTTTTTT GGGAAGAACT GTAACAAACC ATGCAACTGC
    GCCAACAACG GCTATTGCCA CAGACTGTAC GGAGCCTGCC TGTGTGACCC CGGGCTCTAC
    GGGCGCTTCT GCCACCTCAC CTGTCCCAGG TGGGCCTTCG GAGCCGGCTG CTCGGAGGAG
    TGTCAGTGCG TGAAGGAGCA CACGCTGGAA TGCAATGCCA GGAACGGGAC TTGCACCTGC
    AAGCCAGGTT ACCACGGCAA AAAGTGTCAG AAAGAGTGCA TGCCTGGCTT TTATGGGGCT
    GGTTGCAAAC TGAGGTGCAA CTGTCCTCCT GATGTGTCGT GTAACCGTGA GACGGGAGCC
    TGCCAGCATC CGTGTCCACC TGGGTTTCAT GGAGAAGAAT GCCATTTATC TTGTCAGCCC
    GGGAGCTTTG GGGTGAACTG CGGGCAGAGG TGCAGCTGTG GAGAAGCACC GTGTGATCCA
    AAGACGGGGC AGTGCATTTG CCCAGCTGGG AAGGCTGGTG CTACATGTGA GCAAGATTGC
    CCAGCGGGTC GGTGGGGACC AGACTGCCAG TCCTCCTGTG AGCCCTGTGC CAACGGGGGC
    CAGTGCAACA GAGAAACTGG AGCCTGCGAC TGTCCCCCGG GCTACACCGG GGCATCCTGC
    TCTGCCTCCT GCCCTGCTGG TTACTACGGA CAAAACTGCC TGCTGTCGTG CAGCTGTAGC
    AGTGGTGCTC AGTGTCACCA TGTCACCGGA GAGTGCACGT GTCCCCCAGG CTGGACCGGC
    CCCGACTGCA AACACCCCTG CAACAGTGGC CGTTGGGGCC TGCGCTGTGA AAACACGTGT
    GTCTGCAATA ACAGCGACGG TAGCTGCGAT CCCGTCACCG GCTCCTGCTT CTGTGAGCCA
    GGGTTCACTG GGGAACACTG CGAATGGAGG TGCCCCGAGG GAAGCTTTGG CCCGAGCTGT
    GAGTCCAGCT GCCAGTGCCA GAATGGAGCT GCCTGCGACC ACGTCAGCGG AGCCTGTACC
    TGCACGGCAG GCTGGACAGG AACCTTTTGT GAAAGAGCAT GTCCAGATGG ATTCTTTGGG
    CTTGACTGCC ACCAGGTGTG CGAGTGCAAG AATGCTGCTG GCTGCAACCA CGTCCTGGGA
    ACCTGCCGCT GCCTCCCGGG CTGGCGGGGA GAGACCTGCC AGCAGCCCTG CCAGGGGAAC
    AGCTATGGGC CGGGCTGCAA CCACACTTGT CACTGTCACA ACGGAGCCCT CTGTCATCAT
    GTAACCGGGA CGTGCCTTTG TAGCCCAGGG TGGAAAGGAA CCACCTGTCA AGAAGCCTGT
    CCTCCTGGAT GGTATGGTGC AAACTGTCTC CAGCGCTGCG TCTGCCACGG CCAGGCGACG
    TGTGACCGTG TGACGGGCGA GTGCCGGTGT CCCCAGGGTT GGATGGGGAT AGCCTGTGAG
    CTGGAGTGTG GGGACGGGAA GCACGGAGAG GGCTGTGGGC AGAACTGCAG CTGCCACCGT
    GGGGTTTGTG ACCAGCACTC AGGGAGGTGC ATCTGCCACG CCGGCTGGAC TGGAGACCGC
    TGCGATGTTG CCTGTCCCCC GGGATCTTTT GGCAAGCAGT GCGAGGAGCG GTGTGACTGC
    GTGCACGGCT TGTCCTGCCA CCATCAAACA GGAGCGTGTC ACTGTGCAAA GGGGTGGCGA
    GGGAGGCACT GTGACAAACC TTGCTTGCCT GGACGCTACG GTGTGGGCTG TGCCCAGCGG
    TGCCGGTGTC CTGCGGGTAC CCCCTGCCAC CACCTGACGG GCAAGTGCGG CTGTCCTCCG
    GGATTCACCG GCTACGGCTG CGAGAAAACT TGTCCACCGG GCACGTATGG TAAGAACTGT
    AACCGAGTGT GTCAGTGCTC TGCCGAGAAT GAGGAATGTC ACCCGGTGAC CGGCGACTGT
    GTGTGCCACC CGGGCTACCA CGGGGCCCGC TGCACCCTCC GGTGTCCAAA GGGTACTTAC
    GGTCCAAACT GCCAAAACCC CTGTAAATGC ATGAATGGAG GCCATTGCGA CCCCGTGTCG
    GGAACTTGTG ATTGCACGCC TGGTTTCATT GGAGCCGACT GTAGCAAAAC TTGCCCTGAA
    AACCGATACG GGAAGGACTG CACCCTGTTC TGTGCATGTG GTAAAGGTCA GTGTGACCCA
    CGGACTGGCA AGTGTTCCTG TCCTCCCGGC CAAATGGGAC CCAGCTGTCA GCAAGTGTGT
    CCCCAGGGAC AATACGGCCC CAACTGCCAG CTGACATGTA CCTGTCAGAA CGGTGGTATC
    TGTGACCACG TGGATGGCAA CTGCACTTGT GGGCTCGGCT GGACAGGAAG ATCGTGTGAG
    AAAGCAGAAT GTCTCCCAGG GAAGTACGGG TCTGGCTGTG GCTTGGACTG TTCGTGCCAG
    CACAACGGCA CCTGCGACAG GTTCACGGGC TGCTGCCGGT GCCCCGAGGG CTACTACGGC
    CACTCCTGCG AGCACAGGTG TCCCCTTGGA TTTTATGGAC TAAGCTGTCT TCATGCGTGT
    GCCTGTAAAA ATGGAGGGAG CTGCGATGCG GTGACGGGAC AGTGCATGTG TCCTTTGGGT
    TATCAAGGTG TCCACTGCGA GAAAGGCTGT GACAGGGGCT GGTTTGGAGA GAGGTGCCAG
    CATCAGTGCG ACTGCGGAGA TGCTCCATGT GATCCAGAGA CTGGGAAGTG CCTTTGCCCA
    CCTGGGAAGA CCGGAGATAA ATGTGATGTT GAATGCAGGT CAGACCAGTA TGGCCCCAAC
    TGCTCCTTAC GATGCCAGTG TGCCAGCAAA TCCCAGTGCA ATCCATACAA CGGGAACTGC
    GTCTGCCCGG CCACGTGGAT GGGGCCAACC TGCAAAGAAG GTGGCCCTCC AAAGCTTCCT
    TTTTATGAAG AACAAACTCA AGAAAGAGGG AATATTTTTC CAAGAGCTCT ACAACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa62759
    Clone ID Related Accession (Same CDS sequence) XM_014938860.1
    Accession Version XM_014938860.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product multiple epidermal growth factor-like domains protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090811.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACATAAATG AGTGCACCAC TGAGAACGGT
    GGCTGTCATG ACCAGTGCTG TAATACCATC GGCAGCTACC ACTGCAAATG TCCAGCTGGC
    CAGAAACTGG AGGAAGATGG AAAATCCTGT GGAGACGTGG ACGAGTGCGC GGTGGTGAAC
    GGTGGCTGCC AGCAAGGCTG TGTCAACACA CACGGCTCCT TCCACTGCCA GTGCCGGGCG
    GGATACAGGC TCCACGCTGA TGGGCGCACC TGCATAGCCA TCAATTCCTG CAGTGTTAAC
    AAGGGGGGCT GCGAGCAGGA GTGCCTTCAG CTCGGTGAGG ACCGCTACAA GTGCCAGTGC
    CACCCGGGCT ACCAGCTGAA GAGGGATGCC AAATCCTGTG AAGACGTTGA TGAGTGTGCA
    GAAGGGAGAG CCAGGTGTGC TCATCGTTGT GTGAACACTC CAGGATCCTT CACCTGTGAC
    TGCAACCCCG GCTTCGAGCT GGGGGCTGAC GGGAAGCAGT GCTACCGCAT CGAAATGGAA
    ATTGTCGATA GCTGCCAGGA CAGCAATGGT GGCTGCTCTC ATCACTGCGA GCACACGACT
    GCGGGGCCGC GCTGCTCCTG CGACGACGGC TATCGCCTGG GTGTTGATGG CAAAACGTGT
    GCAGAGGTGG ACGAGTGTGA GACCGGAGAG TCCTGCTGCT CCCAATTCTG CATCAACTAC
    GTGGGAGGCT ACGAGTGTGC CTGCAAAGCG GGGTTCCAGC CCAACGCCGA CGGCTGCGCC
    TGCGACGATG TGGATGAATG TCGTCTTGAT AATGGCAACT GTGACCATTT CTGTGTTAAT
    TCTCTGGGGT CATACGAATG TGCGTGTAAG GAGGGTTACA GGCTTGGTGT TAACAGATGG
    TCCTGCATCG CCCTAGGTGG GGAGGAGACA GATGTGGAGG AGGCGACGGG GTTCGCCGGG
    GCTCCCGGGC TGCAGTTCAG AGGTCCCCCC CAGCTCCTTC ACTACGCCCT CGGTGCCCTC
    TACGAGGATG AAGACCCACG AGGAGAACTC ACCCTGGTGC ACAGGGTCGT TTGCCTCGAT
    AACACTTTTG GCAACGACTG TAGCTTGAGC TGTGAGGATT GTCTGAACGG AGGCAGATGT
    AACAGCGAAG GCAGCGGCTG CGTGTGCTCG CCCGGGTGGA GCGGCATTAT CTGTAATGAA
    ACTTGTTCCC CCGGGACGTA CGGCAGAGGC TGTGCCAGTG TTTGCAAGTG CCAGAACGGA
    GGCTCCTGTG ACCCCGTCAC CGGGCAGTGC CGCTGTCCCC CCGGCGTGCA CGGCAAGCTC
    TGTGAAGATG GTTGCCCAAA GGGTTTTTTT GGGAAGAACT GTAACAAACC ATGCAACTGC
    GCCAACAACG GCTATTGCCA CAGACTGTAC GGAGCCTGCC TGTGTGACCC CGGGCTCTAC
    GGGCGCTTCT GCCACCTCAC CTGTCCCAGG TGGGCCTTCG GAGCCGGCTG CTCGGAGGAG
    TGTCAGTGCG TGAAGGAGCA CACGCTGGAA TGCAATGCCA GGAACGGGAC TTGCACCTGC
    AAGCCAGGTT ACCACGGCAA AAAGTGTCAG AAAGAGTGCA TGCCTGGCTT TTATGGGGCT
    GGTTGCAAAC TGAGGTGCAA CTGTCCTCCT GATGTGTCGT GTAACCGTGA GACGGGAGCC
    TGCCAGCATC CGTGTCCACC TGGGTTTCAT GGAGAAGAAT GCCATTTATC TTGTCAGCCC
    GGGAGCTTTG GGGTGAACTG CGGGCAGAGG TGCAGCTGTG GAGAAGCACC GTGTGATCCA
    AAGACGGGGC AGTGCATTTG CCCAGCTGGG AAGGCTGGTG CTACATGTGA GCAAGATTGC
    CCAGCGGGTC GGTGGGGACC AGACTGCCAG TCCTCCTGTG AGCCCTGTGC CAACGGGGGC
    CAGTGCAACA GAGAAACTGG AGCCTGCGAC TGTCCCCCGG GCTACACCGG GGCATCCTGC
    TCTGCCTCCT GCCCTGCTGG TTACTACGGA CAAAACTGCC TGCTGTCGTG CAGCTGTAGC
    AGTGGTGCTC AGTGTCACCA TGTCACCGGA GAGTGCACGT GTCCCCCAGG CTGGACCGGC
    CCCGACTGCA AACACCCCTG CAACAGTGGC CGTTGGGGCC TGCGCTGTGA AAACACGTGT
    GTCTGCAATA ACAGCGACGG TAGCTGCGAT CCCGTCACCG GCTCCTGCTT CTGTGAGCCA
    GGGTTCACTG GGGAACACTG CGAATGGAGG TGCCCCGAGG GAAGCTTTGG CCCGAGCTGT
    GAGTCCAGCT GCCAGTGCCA GAATGGAGCT GCCTGCGACC ACGTCAGCGG AGCCTGTACC
    TGCACGGCAG GCTGGACAGG AACCTTTTGT GAAAGAGCAT GTCCAGATGG ATTCTTTGGG
    CTTGACTGCC ACCAGGTGTG CGAGTGCAAG AATGCTGCTG GCTGCAACCA CGTCCTGGGA
    ACCTGCCGCT GCCTCCCGGG CTGGCGGGGA GAGACCTGCC AGCAGCCCTG CCAGGGGAAC
    AGCTATGGGC CGGGCTGCAA CCACACTTGT CACTGTCACA ACGGAGCCCT CTGTCATCAT
    GTAACCGGGA CGTGCCTTTG TAGCCCAGGG TGGAAAGGAA CCACCTGTCA AGAAGCCTGT
    CCTCCTGGAT GGTATGGTGC AAACTGTCTC CAGCGCTGCG TCTGCCACGG CCAGGCGACG
    TGTGACCGTG TGACGGGCGA GTGCCGGTGT CCCCAGGGTT GGATGGGGAT AGCCTGTGAG
    CTGGAGTGTG GGGACGGGAA GCACGGAGAG GGCTGTGGGC AGAACTGCAG CTGCCACCGT
    GGGGTTTGTG ACCAGCACTC AGGGAGGTGC ATCTGCCACG CCGGCTGGAC TGGAGACCGC
    TGCGATGTTG CCTGTCCCCC GGGATCTTTT GGCAAGCAGT GCGAGGAGCG GTGTGACTGC
    GTGCACGGCT TGTCCTGCCA CCATCAAACA GGAGCGTGTC ACTGTGCAAA GGGGTGGCGA
    GGGAGGCACT GTGACAAACC TTGCTTGCCT GGACGCTACG GTGTGGGCTG TGCCCAGCGG
    TGCCGGTGTC CTGCGGGTAC CCCCTGCCAC CACCTGACGG GCAAGTGCGG CTGTCCTCCG
    GGATTCACCG GCTACGGCTG CGAGAAAACT TGTCCACCGG GCACGTATGG TAAGAACTGT
    AACCGAGTGT GTCAGTGCTC TGCCGAGAAT GAGGAATGTC ACCCGGTGAC CGGCGACTGT
    GTGTGCCACC CGGGCTACCA CGGGGCCCGC TGCACCCTCC GGTGTCCAAA GGGTACTTAC
    GGTCCAAACT GCCAAAACCC CTGTAAATGC ATGAATGGAG GCCATTGCGA CCCCGTGTCG
    GGAACTTGTG ATTGCACGCC TGGTTTCATT GGAGCCGACT GTAGCAAAAC TTGCCCTGAA
    AACCGATACG GGAAGGACTG CACCCTGTTC TGTGCATGTG GTAAAGGTCA GTGTGACCCA
    CGGACTGGCA AGTGTTCCTG TCCTCCCGGC CAAATGGGAC CCAGCTGTCA GCAAGTGTGT
    CCCCAGGGAC AATACGGCCC CAACTGCCAG CTGACATGTA CCTGTCAGAA CGGTGGTATC
    TGTGACCACG TGGATGGCAA CTGCACTTGT GGGCTCGGCT GGACAGGAAG ATCGTGTGAG
    AAAGAATGTC TCCCAGGGAA GTACGGGTCT GGCTGTGGCT TGGACTGTTC GTGCCAGCAC
    AACGGCACCT GCGACAGGTT CACGGGCTGC TGCCGGTGCC CCGAGGGCTA CTACGGCCAC
    TCCTGCGAGC ACAGGTGTCC CCTTGGATTT TATGGACTAA GCTGTCTTCA TGCGTGTGCC
    TGTAAAAATG GAGGGAGCTG CGATGCGGTG ACGGGACAGT GCATGTGTCC TTTGGGTTAT
    CAAGGTGTCC ACTGCGAGAA AGGCTGTGAC AGGGGCTGGT TTGGAGAGAG GTGCCAGCAT
    CAGTGCGACT GCGGAGATGC TCCATGTGAT CCAGAGACTG GGAAGTGCCT TTGCCCACCT
    GGGAAGACCG GAGATAAATG TGATGTTGAA TGCAGGTCAG ACCAGTATGG CCCCAACTGC
    TCCTTACGAT GCCAGTGTGC CAGCAAATCC CAGTGCAATC CATACAACGG GAACTGCGTC
    TGCCCGGCCA CGTGGATGGG GCCAACCTGC AAAGAAGGTG GCCCTCCAAA GCTTCCTTTT
    TATGAAGAAC AAACTCAAGA AAGAGGGAAT ATTTTTCCAA GAGCTCTACA ACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014794346.1
    CDS47..4723
    Translation

    Target ORF information:

    RefSeq Version XM_014938860.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014938860.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACATAAATG AGTGCACCAC TGAGAACGGT
    GGCTGTCATG ACCAGTGCTG TAATACCATC GGCAGCTACC ACTGCAAATG TCCAGCTGGC
    CAGAAACTGG AGGAAGATGG AAAATCCTGT GGAGACGTGG ACGAGTGCGC GGTGGTGAAC
    GGTGGCTGCC AGCAAGGCTG TGTCAACACA CACGGCTCCT TCCACTGCCA GTGCCGGGCG
    GGATACAGGC TCCACGCTGA TGGGCGCACC TGCATAGCCA TCAATTCCTG CAGTGTTAAC
    AAGGGGGGCT GCGAGCAGGA GTGCCTTCAG CTCGGTGAGG ACCGCTACAA GTGCCAGTGC
    CACCCGGGCT ACCAGCTGAA GAGGGATGCC AAATCCTGTG AAGACGTTGA TGAGTGTGCA
    GAAGGGAGAG CCAGGTGTGC TCATCGTTGT GTGAACACTC CAGGATCCTT CACCTGTGAC
    TGCAACCCCG GCTTCGAGCT GGGGGCTGAC GGGAAGCAGT GCTACCGCAT CGAAATGGAA
    ATTGTCGATA GCTGCCAGGA CAGCAATGGT GGCTGCTCTC ATCACTGCGA GCACACGACT
    GCGGGGCCGC GCTGCTCCTG CGACGACGGC TATCGCCTGG GTGTTGATGG CAAAACGTGT
    GCAGAGGTGG ACGAGTGTGA GACCGGAGAG TCCTGCTGCT CCCAATTCTG CATCAACTAC
    GTGGGAGGCT ACGAGTGTGC CTGCAAAGCG GGGTTCCAGC CCAACGCCGA CGGCTGCGCC
    TGCGACGATG TGGATGAATG TCGTCTTGAT AATGGCAACT GTGACCATTT CTGTGTTAAT
    TCTCTGGGGT CATACGAATG TGCGTGTAAG GAGGGTTACA GGCTTGGTGT TAACAGATGG
    TCCTGCATCG CCCTAGGTGG GGAGGAGACA GATGTGGAGG AGGCGACGGG GTTCGCCGGG
    GCTCCCGGGC TGCAGTTCAG AGGTCCCCCC CAGCTCCTTC ACTACGCCCT CGGTGCCCTC
    TACGAGGATG AAGACCCACG AGGAGAACTC ACCCTGGTGC ACAGGGTCGT TTGCCTCGAT
    AACACTTTTG GCAACGACTG TAGCTTGAGC TGTGAGGATT GTCTGAACGG AGGCAGATGT
    AACAGCGAAG GCAGCGGCTG CGTGTGCTCG CCCGGGTGGA GCGGCATTAT CTGTAATGAA
    ACTTGTTCCC CCGGGACGTA CGGCAGAGGC TGTGCCAGTG TTTGCAAGTG CCAGAACGGA
    GGCTCCTGTG ACCCCGTCAC CGGGCAGTGC CGCTGTCCCC CCGGCGTGCA CGGCAAGCTC
    TGTGAAGATG GTTGCCCAAA GGGTTTTTTT GGGAAGAACT GTAACAAACC ATGCAACTGC
    GCCAACAACG GCTATTGCCA CAGACTGTAC GGAGCCTGCC TGTGTGACCC CGGGCTCTAC
    GGGCGCTTCT GCCACCTCAC CTGTCCCAGG TGGGCCTTCG GAGCCGGCTG CTCGGAGGAG
    TGTCAGTGCG TGAAGGAGCA CACGCTGGAA TGCAATGCCA GGAACGGGAC TTGCACCTGC
    AAGCCAGGTT ACCACGGCAA AAAGTGTCAG AAAGAGTGCA TGCCTGGCTT TTATGGGGCT
    GGTTGCAAAC TGAGGTGCAA CTGTCCTCCT GATGTGTCGT GTAACCGTGA GACGGGAGCC
    TGCCAGCATC CGTGTCCACC TGGGTTTCAT GGAGAAGAAT GCCATTTATC TTGTCAGCCC
    GGGAGCTTTG GGGTGAACTG CGGGCAGAGG TGCAGCTGTG GAGAAGCACC GTGTGATCCA
    AAGACGGGGC AGTGCATTTG CCCAGCTGGG AAGGCTGGTG CTACATGTGA GCAAGATTGC
    CCAGCGGGTC GGTGGGGACC AGACTGCCAG TCCTCCTGTG AGCCCTGTGC CAACGGGGGC
    CAGTGCAACA GAGAAACTGG AGCCTGCGAC TGTCCCCCGG GCTACACCGG GGCATCCTGC
    TCTGCCTCCT GCCCTGCTGG TTACTACGGA CAAAACTGCC TGCTGTCGTG CAGCTGTAGC
    AGTGGTGCTC AGTGTCACCA TGTCACCGGA GAGTGCACGT GTCCCCCAGG CTGGACCGGC
    CCCGACTGCA AACACCCCTG CAACAGTGGC CGTTGGGGCC TGCGCTGTGA AAACACGTGT
    GTCTGCAATA ACAGCGACGG TAGCTGCGAT CCCGTCACCG GCTCCTGCTT CTGTGAGCCA
    GGGTTCACTG GGGAACACTG CGAATGGAGG TGCCCCGAGG GAAGCTTTGG CCCGAGCTGT
    GAGTCCAGCT GCCAGTGCCA GAATGGAGCT GCCTGCGACC ACGTCAGCGG AGCCTGTACC
    TGCACGGCAG GCTGGACAGG AACCTTTTGT GAAAGAGCAT GTCCAGATGG ATTCTTTGGG
    CTTGACTGCC ACCAGGTGTG CGAGTGCAAG AATGCTGCTG GCTGCAACCA CGTCCTGGGA
    ACCTGCCGCT GCCTCCCGGG CTGGCGGGGA GAGACCTGCC AGCAGCCCTG CCAGGGGAAC
    AGCTATGGGC CGGGCTGCAA CCACACTTGT CACTGTCACA ACGGAGCCCT CTGTCATCAT
    GTAACCGGGA CGTGCCTTTG TAGCCCAGGG TGGAAAGGAA CCACCTGTCA AGAAGCCTGT
    CCTCCTGGAT GGTATGGTGC AAACTGTCTC CAGCGCTGCG TCTGCCACGG CCAGGCGACG
    TGTGACCGTG TGACGGGCGA GTGCCGGTGT CCCCAGGGTT GGATGGGGAT AGCCTGTGAG
    CTGGAGTGTG GGGACGGGAA GCACGGAGAG GGCTGTGGGC AGAACTGCAG CTGCCACCGT
    GGGGTTTGTG ACCAGCACTC AGGGAGGTGC ATCTGCCACG CCGGCTGGAC TGGAGACCGC
    TGCGATGTTG CCTGTCCCCC GGGATCTTTT GGCAAGCAGT GCGAGGAGCG GTGTGACTGC
    GTGCACGGCT TGTCCTGCCA CCATCAAACA GGAGCGTGTC ACTGTGCAAA GGGGTGGCGA
    GGGAGGCACT GTGACAAACC TTGCTTGCCT GGACGCTACG GTGTGGGCTG TGCCCAGCGG
    TGCCGGTGTC CTGCGGGTAC CCCCTGCCAC CACCTGACGG GCAAGTGCGG CTGTCCTCCG
    GGATTCACCG GCTACGGCTG CGAGAAAACT TGTCCACCGG GCACGTATGG TAAGAACTGT
    AACCGAGTGT GTCAGTGCTC TGCCGAGAAT GAGGAATGTC ACCCGGTGAC CGGCGACTGT
    GTGTGCCACC CGGGCTACCA CGGGGCCCGC TGCACCCTCC GGTGTCCAAA GGGTACTTAC
    GGTCCAAACT GCCAAAACCC CTGTAAATGC ATGAATGGAG GCCATTGCGA CCCCGTGTCG
    GGAACTTGTG ATTGCACGCC TGGTTTCATT GGAGCCGACT GTAGCAAAAC TTGCCCTGAA
    AACCGATACG GGAAGGACTG CACCCTGTTC TGTGCATGTG GTAAAGGTCA GTGTGACCCA
    CGGACTGGCA AGTGTTCCTG TCCTCCCGGC CAAATGGGAC CCAGCTGTCA GCAAGTGTGT
    CCCCAGGGAC AATACGGCCC CAACTGCCAG CTGACATGTA CCTGTCAGAA CGGTGGTATC
    TGTGACCACG TGGATGGCAA CTGCACTTGT GGGCTCGGCT GGACAGGAAG ATCGTGTGAG
    AAAGAATGTC TCCCAGGGAA GTACGGGTCT GGCTGTGGCT TGGACTGTTC GTGCCAGCAC
    AACGGCACCT GCGACAGGTT CACGGGCTGC TGCCGGTGCC CCGAGGGCTA CTACGGCCAC
    TCCTGCGAGC ACAGGTGTCC CCTTGGATTT TATGGACTAA GCTGTCTTCA TGCGTGTGCC
    TGTAAAAATG GAGGGAGCTG CGATGCGGTG ACGGGACAGT GCATGTGTCC TTTGGGTTAT
    CAAGGTGTCC ACTGCGAGAA AGGCTGTGAC AGGGGCTGGT TTGGAGAGAG GTGCCAGCAT
    CAGTGCGACT GCGGAGATGC TCCATGTGAT CCAGAGACTG GGAAGTGCCT TTGCCCACCT
    GGGAAGACCG GAGATAAATG TGATGTTGAA TGCAGGTCAG ACCAGTATGG CCCCAACTGC
    TCCTTACGAT GCCAGTGTGC CAGCAAATCC CAGTGCAATC CATACAACGG GAACTGCGTC
    TGCCCGGCCA CGTGGATGGG GCCAACCTGC AAAGAAGGTG GCCCTCCAAA GCTTCCTTTT
    TATGAAGAAC AAACTCAAGA AAGAGGGAAT ATTTTTCCAA GAGCTCTACA ACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa62760
    Clone ID Related Accession (Same CDS sequence) XM_014938861.1
    Accession Version XM_014938861.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product multiple epidermal growth factor-like domains protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090811.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACGTGGACG AGTGCGCGGT GGTGAACGGT
    GGCTGCCAGC AAGGCTGTGT CAACACACAC GGCTCCTTCC ACTGCCAGTG CCGGGCGGGA
    TACAGGCTCC ACGCTGATGG GCGCACCTGC ATAGCCATCA ATTCCTGCAG TGTTAACAAG
    GGGGGCTGCG AGCAGGAGTG CCTTCAGCTC GGTGAGGACC GCTACAAGTG CCAGTGCCAC
    CCGGGCTACC AGCTGAAGAG GGATGCCAAA TCCTGTGAAG ACGTTGATGA GTGTGCAGAA
    GGGAGAGCCA GGTGTGCTCA TCGTTGTGTG AACACTCCAG GATCCTTCAC CTGTGACTGC
    AACCCCGGCT TCGAGCTGGG GGCTGACGGG AAGCAGTGCT ACCGCATCGA AATGGAAATT
    GTCGATAGCT GCCAGGACAG CAATGGTGGC TGCTCTCATC ACTGCGAGCA CACGACTGCG
    GGGCCGCGCT GCTCCTGCGA CGACGGCTAT CGCCTGGGTG TTGATGGCAA AACGTGTGCA
    GAGGTGGACG AGTGTGAGAC CGGAGAGTCC TGCTGCTCCC AATTCTGCAT CAACTACGTG
    GGAGGCTACG AGTGTGCCTG CAAAGCGGGG TTCCAGCCCA ACGCCGACGG CTGCGCCTGC
    GACGATGTGG ATGAATGTCG TCTTGATAAT GGCAACTGTG ACCATTTCTG TGTTAATTCT
    CTGGGGTCAT ACGAATGTGC GTGTAAGGAG GGTTACAGGC TTGGTGTTAA CAGATGGTCC
    TGCATCGCCC TAGGTGGGGA GGAGACAGAT GTGGAGGAGG CGACGGGGTT CGCCGGGGCT
    CCCGGGCTGC AGTTCAGAGG TCCCCCCCAG CTCCTTCACT ACGCCCTCGG TGCCCTCTAC
    GAGGATGAAG ACCCACGAGG AGAACTCACC CTGGTGCACA GGGTCGTTTG CCTCGATAAC
    ACTTTTGGCA ACGACTGTAG CTTGAGCTGT GAGGATTGTC TGAACGGAGG CAGATGTAAC
    AGCGAAGGCA GCGGCTGCGT GTGCTCGCCC GGGTGGAGCG GCATTATCTG TAATGAAACT
    TGTTCCCCCG GGACGTACGG CAGAGGCTGT GCCAGTGTTT GCAAGTGCCA GAACGGAGGC
    TCCTGTGACC CCGTCACCGG GCAGTGCCGC TGTCCCCCCG GCGTGCACGG CAAGCTCTGT
    GAAGATGGTT GCCCAAAGGG TTTTTTTGGG AAGAACTGTA ACAAACCATG CAACTGCGCC
    AACAACGGCT ATTGCCACAG ACTGTACGGA GCCTGCCTGT GTGACCCCGG GCTCTACGGG
    CGCTTCTGCC ACCTCACCTG TCCCAGGTGG GCCTTCGGAG CCGGCTGCTC GGAGGAGTGT
    CAGTGCGTGA AGGAGCACAC GCTGGAATGC AATGCCAGGA ACGGGACTTG CACCTGCAAG
    CCAGGTTACC ACGGCAAAAA GTGTCAGAAA GAGTGCATGC CTGGCTTTTA TGGGGCTGGT
    TGCAAACTGA GGTGCAACTG TCCTCCTGAT GTGTCGTGTA ACCGTGAGAC GGGAGCCTGC
    CAGCATCCGT GTCCACCTGG GTTTCATGGA GAAGAATGCC ATTTATCTTG TCAGCCCGGG
    AGCTTTGGGG TGAACTGCGG GCAGAGGTGC AGCTGTGGAG AAGCACCGTG TGATCCAAAG
    ACGGGGCAGT GCATTTGCCC AGCTGGGAAG GCTGGTGCTA CATGTGAGCA AGATTGCCCA
    GCGGGTCGGT GGGGACCAGA CTGCCAGTCC TCCTGTGAGC CCTGTGCCAA CGGGGGCCAG
    TGCAACAGAG AAACTGGAGC CTGCGACTGT CCCCCGGGCT ACACCGGGGC ATCCTGCTCT
    GCCTCCTGCC CTGCTGGTTA CTACGGACAA AACTGCCTGC TGTCGTGCAG CTGTAGCAGT
    GGTGCTCAGT GTCACCATGT CACCGGAGAG TGCACGTGTC CCCCAGGCTG GACCGGCCCC
    GACTGCAAAC ACCCCTGCAA CAGTGGCCGT TGGGGCCTGC GCTGTGAAAA CACGTGTGTC
    TGCAATAACA GCGACGGTAG CTGCGATCCC GTCACCGGCT CCTGCTTCTG TGAGCCAGGG
    TTCACTGGGG AACACTGCGA ATGGAGGTGC CCCGAGGGAA GCTTTGGCCC GAGCTGTGAG
    TCCAGCTGCC AGTGCCAGAA TGGAGCTGCC TGCGACCACG TCAGCGGAGC CTGTACCTGC
    ACGGCAGGCT GGACAGGAAC CTTTTGTGAA AGAGCATGTC CAGATGGATT CTTTGGGCTT
    GACTGCCACC AGGTGTGCGA GTGCAAGAAT GCTGCTGGCT GCAACCACGT CCTGGGAACC
    TGCCGCTGCC TCCCGGGCTG GCGGGGAGAG ACCTGCCAGC AGCCCTGCCA GGGGAACAGC
    TATGGGCCGG GCTGCAACCA CACTTGTCAC TGTCACAACG GAGCCCTCTG TCATCATGTA
    ACCGGGACGT GCCTTTGTAG CCCAGGGTGG AAAGGAACCA CCTGTCAAGA AGCCTGTCCT
    CCTGGATGGT ATGGTGCAAA CTGTCTCCAG CGCTGCGTCT GCCACGGCCA GGCGACGTGT
    GACCGTGTGA CGGGCGAGTG CCGGTGTCCC CAGGGTTGGA TGGGGATAGC CTGTGAGCTG
    GAGTGTGGGG ACGGGAAGCA CGGAGAGGGC TGTGGGCAGA ACTGCAGCTG CCACCGTGGG
    GTTTGTGACC AGCACTCAGG GAGGTGCATC TGCCACGCCG GCTGGACTGG AGACCGCTGC
    GATGTTGCCT GTCCCCCGGG ATCTTTTGGC AAGCAGTGCG AGGAGCGGTG TGACTGCGTG
    CACGGCTTGT CCTGCCACCA TCAAACAGGA GCGTGTCACT GTGCAAAGGG GTGGCGAGGG
    AGGCACTGTG ACAAACCTTG CTTGCCTGGA CGCTACGGTG TGGGCTGTGC CCAGCGGTGC
    CGGTGTCCTG CGGGTACCCC CTGCCACCAC CTGACGGGCA AGTGCGGCTG TCCTCCGGGA
    TTCACCGGCT ACGGCTGCGA GAAAACTTGT CCACCGGGCA CGTATGGTAA GAACTGTAAC
    CGAGTGTGTC AGTGCTCTGC CGAGAATGAG GAATGTCACC CGGTGACCGG CGACTGTGTG
    TGCCACCCGG GCTACCACGG GGCCCGCTGC ACCCTCCGGT GTCCAAAGGG TACTTACGGT
    CCAAACTGCC AAAACCCCTG TAAATGCATG AATGGAGGCC ATTGCGACCC CGTGTCGGGA
    ACTTGTGATT GCACGCCTGG TTTCATTGGA GCCGACTGTA GCAAAACTTG CCCTGAAAAC
    CGATACGGGA AGGACTGCAC CCTGTTCTGT GCATGTGGTA AAGGTCAGTG TGACCCACGG
    ACTGGCAAGT GTTCCTGTCC TCCCGGCCAA ATGGGACCCA GCTGTCAGCA AGTGTGTCCC
    CAGGGACAAT ACGGCCCCAA CTGCCAGCTG ACATGTACCT GTCAGAACGG TGGTATCTGT
    GACCACGTGG ATGGCAACTG CACTTGTGGG CTCGGCTGGA CAGGAAGATC GTGTGAGAAA
    GCAGAATGTC TCCCAGGGAA GTACGGGTCT GGCTGTGGCT TGGACTGTTC GTGCCAGCAC
    AACGGCACCT GCGACAGGTT CACGGGCTGC TGCCGGTGCC CCGAGGGCTA CTACGGCCAC
    TCCTGCGAGC ACAGGTGTCC CCTTGGATTT TATGGACTAA GCTGTCTTCA TGCGTGTGCC
    TGTAAAAATG GAGGGAGCTG CGATGCGGTG ACGGGACAGT GCATGTGTCC TTTGGGTTAT
    CAAGGTGTCC ACTGCGAGAA AGGCTGTGAC AGGGGCTGGT TTGGAGAGAG GTGCCAGCAT
    CAGTGCGACT GCGGAGATGC TCCATGTGAT CCAGAGACTG GGAAGTGCCT TTGCCCACCT
    GGGAAGACCG GAGATAAATG TGATGTTGAA TGCAGGTCAG ACCAGTATGG CCCCAACTGC
    TCCTTACGAT GCCAGTGTGC CAGCAAATCC CAGTGCAATC CATACAACGG GAACTGCGTC
    TGCCCGGCCA CGTGGATGGG GCCAACCTGC AAAGAAGGTG GCCCTCCAAA GCTTCCTTTT
    TATGAAGAAC AAACTCAAGA AAGAGGGAAT ATTTTTCCAA GAGCTCTACA ACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014794347.1
    CDS47..4603
    Translation

    Target ORF information:

    RefSeq Version XM_014938861.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014938861.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACGTGGACG AGTGCGCGGT GGTGAACGGT
    GGCTGCCAGC AAGGCTGTGT CAACACACAC GGCTCCTTCC ACTGCCAGTG CCGGGCGGGA
    TACAGGCTCC ACGCTGATGG GCGCACCTGC ATAGCCATCA ATTCCTGCAG TGTTAACAAG
    GGGGGCTGCG AGCAGGAGTG CCTTCAGCTC GGTGAGGACC GCTACAAGTG CCAGTGCCAC
    CCGGGCTACC AGCTGAAGAG GGATGCCAAA TCCTGTGAAG ACGTTGATGA GTGTGCAGAA
    GGGAGAGCCA GGTGTGCTCA TCGTTGTGTG AACACTCCAG GATCCTTCAC CTGTGACTGC
    AACCCCGGCT TCGAGCTGGG GGCTGACGGG AAGCAGTGCT ACCGCATCGA AATGGAAATT
    GTCGATAGCT GCCAGGACAG CAATGGTGGC TGCTCTCATC ACTGCGAGCA CACGACTGCG
    GGGCCGCGCT GCTCCTGCGA CGACGGCTAT CGCCTGGGTG TTGATGGCAA AACGTGTGCA
    GAGGTGGACG AGTGTGAGAC CGGAGAGTCC TGCTGCTCCC AATTCTGCAT CAACTACGTG
    GGAGGCTACG AGTGTGCCTG CAAAGCGGGG TTCCAGCCCA ACGCCGACGG CTGCGCCTGC
    GACGATGTGG ATGAATGTCG TCTTGATAAT GGCAACTGTG ACCATTTCTG TGTTAATTCT
    CTGGGGTCAT ACGAATGTGC GTGTAAGGAG GGTTACAGGC TTGGTGTTAA CAGATGGTCC
    TGCATCGCCC TAGGTGGGGA GGAGACAGAT GTGGAGGAGG CGACGGGGTT CGCCGGGGCT
    CCCGGGCTGC AGTTCAGAGG TCCCCCCCAG CTCCTTCACT ACGCCCTCGG TGCCCTCTAC
    GAGGATGAAG ACCCACGAGG AGAACTCACC CTGGTGCACA GGGTCGTTTG CCTCGATAAC
    ACTTTTGGCA ACGACTGTAG CTTGAGCTGT GAGGATTGTC TGAACGGAGG CAGATGTAAC
    AGCGAAGGCA GCGGCTGCGT GTGCTCGCCC GGGTGGAGCG GCATTATCTG TAATGAAACT
    TGTTCCCCCG GGACGTACGG CAGAGGCTGT GCCAGTGTTT GCAAGTGCCA GAACGGAGGC
    TCCTGTGACC CCGTCACCGG GCAGTGCCGC TGTCCCCCCG GCGTGCACGG CAAGCTCTGT
    GAAGATGGTT GCCCAAAGGG TTTTTTTGGG AAGAACTGTA ACAAACCATG CAACTGCGCC
    AACAACGGCT ATTGCCACAG ACTGTACGGA GCCTGCCTGT GTGACCCCGG GCTCTACGGG
    CGCTTCTGCC ACCTCACCTG TCCCAGGTGG GCCTTCGGAG CCGGCTGCTC GGAGGAGTGT
    CAGTGCGTGA AGGAGCACAC GCTGGAATGC AATGCCAGGA ACGGGACTTG CACCTGCAAG
    CCAGGTTACC ACGGCAAAAA GTGTCAGAAA GAGTGCATGC CTGGCTTTTA TGGGGCTGGT
    TGCAAACTGA GGTGCAACTG TCCTCCTGAT GTGTCGTGTA ACCGTGAGAC GGGAGCCTGC
    CAGCATCCGT GTCCACCTGG GTTTCATGGA GAAGAATGCC ATTTATCTTG TCAGCCCGGG
    AGCTTTGGGG TGAACTGCGG GCAGAGGTGC AGCTGTGGAG AAGCACCGTG TGATCCAAAG
    ACGGGGCAGT GCATTTGCCC AGCTGGGAAG GCTGGTGCTA CATGTGAGCA AGATTGCCCA
    GCGGGTCGGT GGGGACCAGA CTGCCAGTCC TCCTGTGAGC CCTGTGCCAA CGGGGGCCAG
    TGCAACAGAG AAACTGGAGC CTGCGACTGT CCCCCGGGCT ACACCGGGGC ATCCTGCTCT
    GCCTCCTGCC CTGCTGGTTA CTACGGACAA AACTGCCTGC TGTCGTGCAG CTGTAGCAGT
    GGTGCTCAGT GTCACCATGT CACCGGAGAG TGCACGTGTC CCCCAGGCTG GACCGGCCCC
    GACTGCAAAC ACCCCTGCAA CAGTGGCCGT TGGGGCCTGC GCTGTGAAAA CACGTGTGTC
    TGCAATAACA GCGACGGTAG CTGCGATCCC GTCACCGGCT CCTGCTTCTG TGAGCCAGGG
    TTCACTGGGG AACACTGCGA ATGGAGGTGC CCCGAGGGAA GCTTTGGCCC GAGCTGTGAG
    TCCAGCTGCC AGTGCCAGAA TGGAGCTGCC TGCGACCACG TCAGCGGAGC CTGTACCTGC
    ACGGCAGGCT GGACAGGAAC CTTTTGTGAA AGAGCATGTC CAGATGGATT CTTTGGGCTT
    GACTGCCACC AGGTGTGCGA GTGCAAGAAT GCTGCTGGCT GCAACCACGT CCTGGGAACC
    TGCCGCTGCC TCCCGGGCTG GCGGGGAGAG ACCTGCCAGC AGCCCTGCCA GGGGAACAGC
    TATGGGCCGG GCTGCAACCA CACTTGTCAC TGTCACAACG GAGCCCTCTG TCATCATGTA
    ACCGGGACGT GCCTTTGTAG CCCAGGGTGG AAAGGAACCA CCTGTCAAGA AGCCTGTCCT
    CCTGGATGGT ATGGTGCAAA CTGTCTCCAG CGCTGCGTCT GCCACGGCCA GGCGACGTGT
    GACCGTGTGA CGGGCGAGTG CCGGTGTCCC CAGGGTTGGA TGGGGATAGC CTGTGAGCTG
    GAGTGTGGGG ACGGGAAGCA CGGAGAGGGC TGTGGGCAGA ACTGCAGCTG CCACCGTGGG
    GTTTGTGACC AGCACTCAGG GAGGTGCATC TGCCACGCCG GCTGGACTGG AGACCGCTGC
    GATGTTGCCT GTCCCCCGGG ATCTTTTGGC AAGCAGTGCG AGGAGCGGTG TGACTGCGTG
    CACGGCTTGT CCTGCCACCA TCAAACAGGA GCGTGTCACT GTGCAAAGGG GTGGCGAGGG
    AGGCACTGTG ACAAACCTTG CTTGCCTGGA CGCTACGGTG TGGGCTGTGC CCAGCGGTGC
    CGGTGTCCTG CGGGTACCCC CTGCCACCAC CTGACGGGCA AGTGCGGCTG TCCTCCGGGA
    TTCACCGGCT ACGGCTGCGA GAAAACTTGT CCACCGGGCA CGTATGGTAA GAACTGTAAC
    CGAGTGTGTC AGTGCTCTGC CGAGAATGAG GAATGTCACC CGGTGACCGG CGACTGTGTG
    TGCCACCCGG GCTACCACGG GGCCCGCTGC ACCCTCCGGT GTCCAAAGGG TACTTACGGT
    CCAAACTGCC AAAACCCCTG TAAATGCATG AATGGAGGCC ATTGCGACCC CGTGTCGGGA
    ACTTGTGATT GCACGCCTGG TTTCATTGGA GCCGACTGTA GCAAAACTTG CCCTGAAAAC
    CGATACGGGA AGGACTGCAC CCTGTTCTGT GCATGTGGTA AAGGTCAGTG TGACCCACGG
    ACTGGCAAGT GTTCCTGTCC TCCCGGCCAA ATGGGACCCA GCTGTCAGCA AGTGTGTCCC
    CAGGGACAAT ACGGCCCCAA CTGCCAGCTG ACATGTACCT GTCAGAACGG TGGTATCTGT
    GACCACGTGG ATGGCAACTG CACTTGTGGG CTCGGCTGGA CAGGAAGATC GTGTGAGAAA
    GCAGAATGTC TCCCAGGGAA GTACGGGTCT GGCTGTGGCT TGGACTGTTC GTGCCAGCAC
    AACGGCACCT GCGACAGGTT CACGGGCTGC TGCCGGTGCC CCGAGGGCTA CTACGGCCAC
    TCCTGCGAGC ACAGGTGTCC CCTTGGATTT TATGGACTAA GCTGTCTTCA TGCGTGTGCC
    TGTAAAAATG GAGGGAGCTG CGATGCGGTG ACGGGACAGT GCATGTGTCC TTTGGGTTAT
    CAAGGTGTCC ACTGCGAGAA AGGCTGTGAC AGGGGCTGGT TTGGAGAGAG GTGCCAGCAT
    CAGTGCGACT GCGGAGATGC TCCATGTGAT CCAGAGACTG GGAAGTGCCT TTGCCCACCT
    GGGAAGACCG GAGATAAATG TGATGTTGAA TGCAGGTCAG ACCAGTATGG CCCCAACTGC
    TCCTTACGAT GCCAGTGTGC CAGCAAATCC CAGTGCAATC CATACAACGG GAACTGCGTC
    TGCCCGGCCA CGTGGATGGG GCCAACCTGC AAAGAAGGTG GCCCTCCAAA GCTTCCTTTT
    TATGAAGAAC AAACTCAAGA AAGAGGGAAT ATTTTTCCAA GAGCTCTACA ACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa62761
    Clone ID Related Accession (Same CDS sequence) XM_014938862.1
    Accession Version XM_014938862.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product multiple epidermal growth factor-like domains protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090811.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACATAAATG AGTGCACCAC TGAGAACGGT
    GGCTGTCATG ACCAGTGCTG TAATACCATC GGCAGCTACC ACTGCAAATG TCCAGCTGGC
    CAGAAACTGG AGGAAGATGG AAAATCCTGT GGAGACGTGG ACGAGTGCGC GGTGGTGAAC
    GGTGGCTGCC AGCAAGGCTG TGTCAACACA CACGGCTCCT TCCACTGCCA GTGCCGGGCG
    GGATACAGGC TCCACGCTGA TGGGCGCACC TGCATAGCCA TCAATTCCTG CAGTGTTAAC
    AAGGGGGGCT GCGAGCAGGA GTGCCTTCAG CTCGGTGAGG ACCGCTACAA GTGCCAGTGC
    CACCCGGGCT ACCAGCTGAA GAGGGATGCC AAATCCTGTG AAGACGTTGA TGAGTGTGCA
    GAAGGGAGAG CCAGGTGTGC TCATCGTTGT GTGAACACTC CAGGATCCTT CACCTGTGAC
    TGCAACCCCG GCTTCGAGCT GGGGGCTGAC GGGAAGCAGT GCTACCGCAT CGAAATGGAA
    ATTGTCGATA GCTGCCAGGA CAGCAATGGT GGCTGCTCTC ATCACTGCGA GCACACGACT
    GCGGGGCCGC GCTGCTCCTG CGACGACGGC TATCGCCTGG GTGTTGATGG CAAAACGTGT
    GCAGAGGTGG ACGAGTGTGA GACCGGAGAG TCCTGCTGCT CCCAATTCTG CATCAACTAC
    GTGGGAGGCT ACGAGTGTGC CTGCAAAGCG GGGTTCCAGC CCAACGCCGA CGGCTGCGCC
    TGCGACGATG TGGATGAATG TCGTCTTGAT AATGGCAACT GTGACCATTT CTGTGTTAAT
    TCTCTGGGGT CATACGAATG TGCGTGTAAG GAGGGTTACA GGCTTGGTGT TAACAGATGG
    TCCTGCATCG CCCTAGGTGG GGAGGAGACA GATGTGGAGG AGGCGACGGG GTTCGCCGGG
    GCTCCCGGGC TGCAGTTCAG AGGTCCCCCC CAGCTCCTTC ACTACGCCCT CGGTGCCCTC
    TACGAGGATG AAGACCCACG AGGAGAACTC ACCCTGGTGC ACAGGGTCGT TTGCCTCGAT
    AACACTTTTG GCAACGACTG TAGCTTGAGC TGTGAGGATT GTCTGAACGG AGGCAGATGT
    AACAGCGAAG GCAGCGGCTG CGTGTGCTCG CCCGGGTGGA GCGGCATTAT CTGTAATGAA
    ACTTGTTCCC CCGGGACGTA CGGCAGAGGC TGTGCCAGTG TTTGCAAGTG CCAGAACGGA
    GGCTCCTGTG ACCCCGTCAC CGGGCAGTGC CGCTGTCCCC CCGGCGTGCA CGGCAAGCTC
    TGTGAAGATG GTTGCCCAAA GGGTTTTTTT GGGAAGAACT GTAACAAACC ATGCAACTGC
    GCCAACAACG GCTATTGCCA CAGACTGTAC GGAGCCTGCC TGTGTGACCC CGGGCTCTAC
    GGGCGCTTCT GCCACCTCAC CTGTCCCAGG TGGGCCTTCG GAGCCGGCTG CTCGGAGGAG
    TGTCAGTGCG TGAAGGAGCA CACGCTGGAA TGCAATGCCA GGAACGGGAC TTGCACCTGC
    AAGCCAGGTT ACCACGGCAA AAAGTGTCAG AAAGAGTGCA TGCCTGGCTT TTATGGGGCT
    GGTTGCAAAC TGAGGTGCAA CTGTCCTCCT GATGTGTCGT GTAACCGTGA GACGGGAGCC
    TGCCAGCATC CGTGTCCACC TGGGTTTCAT GGAGAAGAAT GCCATTTATC TTGTCAGCCC
    GGGAGCTTTG GGGTGAACTG CGGGCAGAGG TGCAGCTGTG GAGAAGCACC GTGTGATCCA
    AAGACGGGGC AGTGCATTTG CCCAGCTGGG AAGGCTGGTG CTACATGTGA GCAAGATTGC
    CCAGCGGGTC GGTGGGGACC AGACTGCCAG TCCTCCTGTG AGCCCTGTGC CAACGGGGGC
    CAGTGCAACA GAGAAACTGG AGCCTGCGAC TGTCCCCCGG GCTACACCGG GGCATCCTGC
    TCTGCCTCCT GCCCTGCTGG TTACTACGGA CAAAACTGCC TGCTGTCGTG CAGCTGTAGC
    AGTGGTGCTC AGTGTCACCA TGTCACCGGA GAGTGCACGT GTCCCCCAGG CTGGACCGGC
    CCCGACTGCA AACACCCCTG CAACAGTGGC CGTTGGGGCC TGCGCTGTGA AAACACGTGT
    GTCTGCAATA ACAGCGACGG TAGCTGCGAT CCCGTCACCG GCTCCTGCTT CTGTGAGCCA
    GGGTTCACTG GGGAACACTG CGAATGGAGG TGCCCCGAGG GAAGCTTTGG CCCGAGCTGT
    GAGTCCAGCT GCCAGTGCCA GAATGGAGCT GCCTGCGACC ACGTCAGCGG AGCCTGTACC
    TGCACGGCAG GCTGGACAGG AACCTTTTGT GAAAGAGCAT GTCCAGATGG ATTCTTTGGG
    CTTGACTGCC ACCAGGTGTG CGAGTGCAAG AATGCTGCTG GCTGCAACCA CGTCCTGGGA
    ACCTGCCGCT GCCTCCCGGG CTGGCGGGGA GAGACCTGCC AGCAGCCCTG CCAGGGGAAC
    AGCTATGGGC CGGGCTGCAA CCACACTTGT CACTGTCACA ACGGAGCCCT CTGTCATCAT
    GTAACCGGGA CGTGCCTTTG TAGCCCAGGG TGGAAAGGAA CCACCTGTCA AGAAGCCTGT
    CCTCCTGGAT GGTATGGTGC AAACTGTCTC CAGCGCTGCG TCTGCCACGG CCAGGCGACG
    TGTGACCGTG TGACGGGCGA GTGCCGGTGT CCCCAGGGTT GGATGGGGAT AGCCTGTGAG
    CTGGAGTGTG GGGACGGGAA GCACGGAGAG GGCTGTGGGC AGAACTGCAG CTGCCACCGT
    GGGGTTTGTG ACCAGCACTC AGGGAGGTGC ATCTGCCACG CCGGCTGGAC TGGAGACCGC
    TGCGATGTTG CTTGCTTGCC TGGACGCTAC GGTGTGGGCT GTGCCCAGCG GTGCCGGTGT
    CCTGCGGGTA CCCCCTGCCA CCACCTGACG GGCAAGTGCG GCTGTCCTCC GGGATTCACC
    GGCTACGGCT GCGAGAAAAC TTGTCCACCG GGCACGTATG GTAAGAACTG TAACCGAGTG
    TGTCAGTGCT CTGCCGAGAA TGAGGAATGT CACCCGGTGA CCGGCGACTG TGTGTGCCAC
    CCGGGCTACC ACGGGGCCCG CTGCACCCTC CGGTGTCCAA AGGGTACTTA CGGTCCAAAC
    TGCCAAAACC CCTGTAAATG CATGAATGGA GGCCATTGCG ACCCCGTGTC GGGAACTTGT
    GATTGCACGC CTGGTTTCAT TGGAGCCGAC TGTAGCAAAA CTTGCCCTGA AAACCGATAC
    GGGAAGGACT GCACCCTGTT CTGTGCATGT GGTAAAGGTC AGTGTGACCC ACGGACTGGC
    AAGTGTTCCT GTCCTCCCGG CCAAATGGGA CCCAGCTGTC AGCAAGTGTG TCCCCAGGGA
    CAATACGGCC CCAACTGCCA GCTGACATGT ACCTGTCAGA ACGGTGGTAT CTGTGACCAC
    GTGGATGGCA ACTGCACTTG TGGGCTCGGC TGGACAGGAA GATCGTGTGA GAAAGCAGAA
    TGTCTCCCAG GGAAGTACGG GTCTGGCTGT GGCTTGGACT GTTCGTGCCA GCACAACGGC
    ACCTGCGACA GGTTCACGGG CTGCTGCCGG TGCCCCGAGG GCTACTACGG CCACTCCTGC
    GAGCACAGGT GTCCCCTTGG ATTTTATGGA CTAAGCTGTC TTCATGCGTG TGCCTGTAAA
    AATGGAGGGA GCTGCGATGC GGTGACGGGA CAGTGCATGT GTCCTTTGGG TTATCAAGGT
    GTCCACTGCG AGAAAGGCTG TGACAGGGGC TGGTTTGGAG AGAGGTGCCA GCATCAGTGC
    GACTGCGGAG ATGCTCCATG TGATCCAGAG ACTGGGAAGT GCCTTTGCCC ACCTGGGAAG
    ACCGGAGATA AATGTGATGT TGAATGCAGG TCAGACCAGT ATGGCCCCAA CTGCTCCTTA
    CGATGCCAGT GTGCCAGCAA ATCCCAGTGC AATCCATACA ACGGGAACTG CGTCTGCCCG
    GCCACGTGGA TGGGGCCAAC CTGCAAAGAA GGTGGCCCTC CAAAGCTTCC TTTTTATGAA
    GAACAAACTC AAGAAAGAGG GAATATTTTT CCAAGAGCTC TACAACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014794348.1
    CDS47..4597
    Translation

    Target ORF information:

    RefSeq Version XM_014938862.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax multiple epidermal growth factor-like domains protein 6 (LOC106886152), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014938862.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG GAGCGGGGCC GGCGTTCCTG TCCCTGGGGC TGTGCTGGGC GCTGGTGGCC 
    CGGGCGAGCG GCGCGGAGGA GCGGGGGCTG CAGCGGGGCA TGCCAAACGT CTGTCCAGTG
    TTGGAGTTGG CTCTGGTTGG ACACCAACAG CCGTGTGTTC AAGCCTTTAG TCGTATGGTC
    AAGATGTGGA AGCAAGGTTG CACAGGGAGG AGGTGGTGTA TGGGCTATGA GAGAAGGTCA
    GGGTACTACA CGGTGTACAA GCAGGCGTAC AGAATGGAGC ATCAAACTGC CTACAAGTGC
    TGCCCTGGGT GGGTCCAACG TGACAATGAG CCTGGCTGTT TGCACTCTCT GTGTTCTGTT
    GGCACTTGCT TCAATGGAGG GAAATGTTCT GAAGCAGGAT CCCAGATGTG CCAGTGTCCT
    GCAGGATTTC AGGGTCCTCG CTGCCAGTAT GACATAAATG AGTGCACCAC TGAGAACGGT
    GGCTGTCATG ACCAGTGCTG TAATACCATC GGCAGCTACC ACTGCAAATG TCCAGCTGGC
    CAGAAACTGG AGGAAGATGG AAAATCCTGT GGAGACGTGG ACGAGTGCGC GGTGGTGAAC
    GGTGGCTGCC AGCAAGGCTG TGTCAACACA CACGGCTCCT TCCACTGCCA GTGCCGGGCG
    GGATACAGGC TCCACGCTGA TGGGCGCACC TGCATAGCCA TCAATTCCTG CAGTGTTAAC
    AAGGGGGGCT GCGAGCAGGA GTGCCTTCAG CTCGGTGAGG ACCGCTACAA GTGCCAGTGC
    CACCCGGGCT ACCAGCTGAA GAGGGATGCC AAATCCTGTG AAGACGTTGA TGAGTGTGCA
    GAAGGGAGAG CCAGGTGTGC TCATCGTTGT GTGAACACTC CAGGATCCTT CACCTGTGAC
    TGCAACCCCG GCTTCGAGCT GGGGGCTGAC GGGAAGCAGT GCTACCGCAT CGAAATGGAA
    ATTGTCGATA GCTGCCAGGA CAGCAATGGT GGCTGCTCTC ATCACTGCGA GCACACGACT
    GCGGGGCCGC GCTGCTCCTG CGACGACGGC TATCGCCTGG GTGTTGATGG CAAAACGTGT
    GCAGAGGTGG ACGAGTGTGA GACCGGAGAG TCCTGCTGCT CCCAATTCTG CATCAACTAC
    GTGGGAGGCT ACGAGTGTGC CTGCAAAGCG GGGTTCCAGC CCAACGCCGA CGGCTGCGCC
    TGCGACGATG TGGATGAATG TCGTCTTGAT AATGGCAACT GTGACCATTT CTGTGTTAAT
    TCTCTGGGGT CATACGAATG TGCGTGTAAG GAGGGTTACA GGCTTGGTGT TAACAGATGG
    TCCTGCATCG CCCTAGGTGG GGAGGAGACA GATGTGGAGG AGGCGACGGG GTTCGCCGGG
    GCTCCCGGGC TGCAGTTCAG AGGTCCCCCC CAGCTCCTTC ACTACGCCCT CGGTGCCCTC
    TACGAGGATG AAGACCCACG AGGAGAACTC ACCCTGGTGC ACAGGGTCGT TTGCCTCGAT
    AACACTTTTG GCAACGACTG TAGCTTGAGC TGTGAGGATT GTCTGAACGG AGGCAGATGT
    AACAGCGAAG GCAGCGGCTG CGTGTGCTCG CCCGGGTGGA GCGGCATTAT CTGTAATGAA
    ACTTGTTCCC CCGGGACGTA CGGCAGAGGC TGTGCCAGTG TTTGCAAGTG CCAGAACGGA
    GGCTCCTGTG ACCCCGTCAC CGGGCAGTGC CGCTGTCCCC CCGGCGTGCA CGGCAAGCTC
    TGTGAAGATG GTTGCCCAAA GGGTTTTTTT GGGAAGAACT GTAACAAACC ATGCAACTGC
    GCCAACAACG GCTATTGCCA CAGACTGTAC GGAGCCTGCC TGTGTGACCC CGGGCTCTAC
    GGGCGCTTCT GCCACCTCAC CTGTCCCAGG TGGGCCTTCG GAGCCGGCTG CTCGGAGGAG
    TGTCAGTGCG TGAAGGAGCA CACGCTGGAA TGCAATGCCA GGAACGGGAC TTGCACCTGC
    AAGCCAGGTT ACCACGGCAA AAAGTGTCAG AAAGAGTGCA TGCCTGGCTT TTATGGGGCT
    GGTTGCAAAC TGAGGTGCAA CTGTCCTCCT GATGTGTCGT GTAACCGTGA GACGGGAGCC
    TGCCAGCATC CGTGTCCACC TGGGTTTCAT GGAGAAGAAT GCCATTTATC TTGTCAGCCC
    GGGAGCTTTG GGGTGAACTG CGGGCAGAGG TGCAGCTGTG GAGAAGCACC GTGTGATCCA
    AAGACGGGGC AGTGCATTTG CCCAGCTGGG AAGGCTGGTG CTACATGTGA GCAAGATTGC
    CCAGCGGGTC GGTGGGGACC AGACTGCCAG TCCTCCTGTG AGCCCTGTGC CAACGGGGGC
    CAGTGCAACA GAGAAACTGG AGCCTGCGAC TGTCCCCCGG GCTACACCGG GGCATCCTGC
    TCTGCCTCCT GCCCTGCTGG TTACTACGGA CAAAACTGCC TGCTGTCGTG CAGCTGTAGC
    AGTGGTGCTC AGTGTCACCA TGTCACCGGA GAGTGCACGT GTCCCCCAGG CTGGACCGGC
    CCCGACTGCA AACACCCCTG CAACAGTGGC CGTTGGGGCC TGCGCTGTGA AAACACGTGT
    GTCTGCAATA ACAGCGACGG TAGCTGCGAT CCCGTCACCG GCTCCTGCTT CTGTGAGCCA
    GGGTTCACTG GGGAACACTG CGAATGGAGG TGCCCCGAGG GAAGCTTTGG CCCGAGCTGT
    GAGTCCAGCT GCCAGTGCCA GAATGGAGCT GCCTGCGACC ACGTCAGCGG AGCCTGTACC
    TGCACGGCAG GCTGGACAGG AACCTTTTGT GAAAGAGCAT GTCCAGATGG ATTCTTTGGG
    CTTGACTGCC ACCAGGTGTG CGAGTGCAAG AATGCTGCTG GCTGCAACCA CGTCCTGGGA
    ACCTGCCGCT GCCTCCCGGG CTGGCGGGGA GAGACCTGCC AGCAGCCCTG CCAGGGGAAC
    AGCTATGGGC CGGGCTGCAA CCACACTTGT CACTGTCACA ACGGAGCCCT CTGTCATCAT
    GTAACCGGGA CGTGCCTTTG TAGCCCAGGG TGGAAAGGAA CCACCTGTCA AGAAGCCTGT
    CCTCCTGGAT GGTATGGTGC AAACTGTCTC CAGCGCTGCG TCTGCCACGG CCAGGCGACG
    TGTGACCGTG TGACGGGCGA GTGCCGGTGT CCCCAGGGTT GGATGGGGAT AGCCTGTGAG
    CTGGAGTGTG GGGACGGGAA GCACGGAGAG GGCTGTGGGC AGAACTGCAG CTGCCACCGT
    GGGGTTTGTG ACCAGCACTC AGGGAGGTGC ATCTGCCACG CCGGCTGGAC TGGAGACCGC
    TGCGATGTTG CTTGCTTGCC TGGACGCTAC GGTGTGGGCT GTGCCCAGCG GTGCCGGTGT
    CCTGCGGGTA CCCCCTGCCA CCACCTGACG GGCAAGTGCG GCTGTCCTCC GGGATTCACC
    GGCTACGGCT GCGAGAAAAC TTGTCCACCG GGCACGTATG GTAAGAACTG TAACCGAGTG
    TGTCAGTGCT CTGCCGAGAA TGAGGAATGT CACCCGGTGA CCGGCGACTG TGTGTGCCAC
    CCGGGCTACC ACGGGGCCCG CTGCACCCTC CGGTGTCCAA AGGGTACTTA CGGTCCAAAC
    TGCCAAAACC CCTGTAAATG CATGAATGGA GGCCATTGCG ACCCCGTGTC GGGAACTTGT
    GATTGCACGC CTGGTTTCAT TGGAGCCGAC TGTAGCAAAA CTTGCCCTGA AAACCGATAC
    GGGAAGGACT GCACCCTGTT CTGTGCATGT GGTAAAGGTC AGTGTGACCC ACGGACTGGC
    AAGTGTTCCT GTCCTCCCGG CCAAATGGGA CCCAGCTGTC AGCAAGTGTG TCCCCAGGGA
    CAATACGGCC CCAACTGCCA GCTGACATGT ACCTGTCAGA ACGGTGGTAT CTGTGACCAC
    GTGGATGGCA ACTGCACTTG TGGGCTCGGC TGGACAGGAA GATCGTGTGA GAAAGCAGAA
    TGTCTCCCAG GGAAGTACGG GTCTGGCTGT GGCTTGGACT GTTCGTGCCA GCACAACGGC
    ACCTGCGACA GGTTCACGGG CTGCTGCCGG TGCCCCGAGG GCTACTACGG CCACTCCTGC
    GAGCACAGGT GTCCCCTTGG ATTTTATGGA CTAAGCTGTC TTCATGCGTG TGCCTGTAAA
    AATGGAGGGA GCTGCGATGC GGTGACGGGA CAGTGCATGT GTCCTTTGGG TTATCAAGGT
    GTCCACTGCG AGAAAGGCTG TGACAGGGGC TGGTTTGGAG AGAGGTGCCA GCATCAGTGC
    GACTGCGGAG ATGCTCCATG TGATCCAGAG ACTGGGAAGT GCCTTTGCCC ACCTGGGAAG
    ACCGGAGATA AATGTGATGT TGAATGCAGG TCAGACCAGT ATGGCCCCAA CTGCTCCTTA
    CGATGCCAGT GTGCCAGCAA ATCCCAGTGC AATCCATACA ACGGGAACTG CGTCTGCCCG
    GCCACGTGGA TGGGGCCAAC CTGCAAAGAA GGTGGCCCTC CAAAGCTTCC TTTTTATGAA
    GAACAAACTC AAGAAAGAGG GAATATTTTT CCAAGAGCTC TACAACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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