MAST2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAST2
Entrez Gene ID	106886357
Full Name	microtubule associated serine/threonine kinase 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014939191.1	XP_014794677.1	microtubule-associated serine/threonine-protein kinase 2 isoform X1
XM_014939193.1	XP_014794679.1	microtubule-associated serine/threonine-protein kinase 2 isoform X3
XM_014939194.1	XP_014794680.1	microtubule-associated serine/threonine-protein kinase 2 isoform X4
XM_014939192.1	XP_014794678.1	microtubule-associated serine/threonine-protein kinase 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following MAST2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAST2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa62991	XM_014939191.1 Latest version!	Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa62992	XM_014939192.1 Latest version!	Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa62993	XM_014939193.1 Latest version!	Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa62994	XM_014939194.1 Latest version!	Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAST2 ( XM_014939191.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794677.1 Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa62991
Clone ID Related Accession (Same CDS sequence)	XM_014939191.1
Accession Version	XM_014939191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090812.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAACAG AGTCCACTCC ATTACGCTGC AGGAAGCTCA GCAATCCTGA CATCTTTTCA 
TCTGCTGGGA AAACCAAGCT CCATCGTCAG CTAAGTCAAG ATGACTGCAA GCTACGAAGA 
GGTAGTTTGG CAAGTTCTTT GTCAGGAAAA CAGCTACTTC CCTTATCAAA TAGTGTCCAC 
AGTGGAGTGG GACAAACAAT ATGGCAGCCA CCTGGAGATA CCTCAAACCT GGTCCGCATG 
AGAAATCAGT CCCTTGGACA ATCTGCTCCT TCACTTACTG CTGGTTTGTC TGGGATTCCT 
GCCAAAAGAG ATTCCATGTT GCTTTTCTTC TATAAAATTT TTCTCCTGGT AAACTTCATC 
TACAGAGCCA CTTCCCTGCC CATACATCTT AATCCATCAC TCCTGAAAGC AAATACTGAC 
AACATGATCT CTGAGGTGAT CCTCCTGTGG AAGGAGCTCA GTCTCCCCAG AAGAGGAAGC 
TTCTGTCGGA CAAGCAACAG AAAGAGTTTG ATCGTAACAT CCAGCACATC TCCAACTCTC 
CCGCGACCAC ATTCCCCTCT TCATGGACAC GCAGGTAGCA GCCCTTTGGA CAGTCCCAGG 
AACTTCTCTC CAAATGCACC TGCTCATTTT TCCTTTGTTC CTGCCCGAAG CCACGGACAC 
AGAGCAGACA GAACGGATGG GCGCCGCTGG TCCTTGGCCT CCCTTCCTTC ATCTGGATAT 
GGAACAAACA CTCCCAGTTC AACAGTTTCA TCATCATGCT CATCTCAGGA GAAGCTGCAC 
CAGCTGCCCT TTCAGCCCAC GGCAGATGAA CTTCATTTCC TGACAAAGCA TTTCAGTACT 
GAGAGCATCA CTGATGAGGA AGGGCGCCAT TCTCCAGCCT TGAGGCCTCG ATCTCGCAGT 
CTCAGCCCGG GTCGCTCCCC AATTTCCTTT GACAGTGAAA TAATAATGAT GAATCATGTG 
TACAAAGAAA GGTTTCCAAA GGCCACGGCG CAGATGGAAG AACGGCTGGC TGAGTTTATT 
GCCTCTAATG CTCCAGAGAA CGTGCTGCAA TTAGCAGATG GGGTCCTAAG TTTCATTCAT 
CATCAAGTTA TTGAACTGGC CAGAGATTGC CTAGATAAAT CACGTGAAGG TCTTATTACT 
TCTCGGTACT TTTATGAGCT GCAGGAAAAC TTGGAGAAAC TTCTCCAGGA TGCCCACGAG 
CGATCCGAGA GCTCCGAGGT TGCCTTCGTT ACCCAACTTG TGAAGAAGCT GATGATTATC 
ATTGCACGGC CGGCTCGGCT GCTGGAGTGC CTGGAATTTG ATCCTGAAGA GTTCTACCAT 
TTGCTAGAAG CTGCAGAAGG CCATGCCAAG GAAGGGCAAG GAATTAAATG TGACATTCCT 
CGTTATATTA TCAGCCAGTT GGGACTCACC AGGGATCCCC TGGAGGAAAT GGCCCAGCTG 
AATAGCTATG ACAGCCCAGA CACTCCTGAG ACAGATGATT CAGTCGAGAG CCGTGGGGCC 
TCTGTCCAGT CAAAGAAAAC TCCATCTGAA GAAGAGTTTG AAACCATTAA ACTGATCAGT 
AATGGTGCTT ATGGAGCGGT GTATTTGGTG CGACACAAGA CCACCCGTCA ACGTTTTGCC 
ATGAAGAAGA TCAACAAACA GAACCTAATT TTGAGAAACC AGATCCAGCA GGCTTTTGTG 
GAGAGAGATA TCTTGACGTT TGCTGAGAAC CCCTTCGTGG TCAGCATGTT CTGCTCCTTT 
GAGACCAAGC GGCACCTGTG CATGGTTATG GAGTATGTGG AAGGAGGTGA TTGTGCCACC 
CTTCTCAAGA ACATCGGTGC CCTGCCTGTT GATATGGCGA GGATGTATTT TGCTGAGACT 
GTTCTGGCAT TGGAGTACCT ACACAATTAT GGGATTGTTC ACCGTGACCT AAAACCTGAT 
AATCTCCTGA TAACTTCCAT GGGTCACATT AAACTAACAG ACTTTGGACT GTCTAAAATT 
GGGTTAATGA GTCTTACAAC TAACCTTTAT GAGGGACACA TTGAGAAGGA TACCAGGGAG 
TTCCTGGACA AGCAGGTCTG TGGGACTCCA GAATACATCG CGCCAGAAGT GATCCTGCGC 
CAGGGCTATG GGAAGCCCGT GGACTGGTGG GCCATGGGAG TTATCTTGTA CGAGTTTCTG 
GTCGGCTGCG TCCCTTTCTT TGGGGACACA CCTGAAGAGC TGTTTGGACA AGTGATCAGT 
GATGAAATTG CCTGGCCAGA AGGTGATGAT GCACTGCCGC CAGATGCCCA GGACCTCATT 
TCTAAGCTTC TCCGCCAAAA TCCTCTGGAA AGAATGGGAA CAGGTAGTGC CTTTGAAGTG 
AAACAGCATC GGTTCTTTAA AGATTTGGAC TGGAATGGAT TACTTCGGCA GAAAGCTGAA 
TTCATCCCAC AGTTGGAATC TGAGGATGAC ACAAGCTATT TCGACACCCG TTCAGAACGG 
TACCAACACC TGGATTCAGA AGAAGAGGAG GACACTAATG ATGATGATCA TGTGGAAATT 
CGGCAATTTT CCTCATGCTC ACCAAGGTTC AGCAAGGTAT ACAGCAGCAT GGAACGTCTT 
TCCATCCACG AAGAGAGGAA GACTCCACCA CCAACTAAAC GGAGTCTGAG TGAAGAAAAA 
GATGACCGGC TGGACAGCCT TGGTGGCTTG AAGAGTCGAG ACCGCAGCTG GGTGATTGGG 
TCTCCTGAAA TACTGCGGAA GCGCTTGTCT ATGTCTGAAT CCTCGCACAC GGAGAGTGAC 
TCCAGCCCAC CCTTGACCGT CCGCCGGCGC TGCTCAGGGC TCCTCGATAT GCCCCGTTTT 
GCCATCTCCA CTGAGGACGA GGGAACTGTT CTCAAAAGGC CACAGTCCGA GGGGATGCTC 
TTATCCACCG TGCAGTCCCG GGAGGGGCTG CCAGTGCCCA TTCCAGAGCA GCCTGTGGAG 
CAAGAGCTGC AGCTGGAAGG TGATGCTGGA CCCACCACCC CCTCCACAGC AGTCAGCAGC 
GCCTCAACGC TGACAGCTGG GAGCACTGCA GATTTCTCTG ATCCACGCGC TCGCAGTAAT 
AGCAATGAAG GCCCAGACTT TACCACTCCA AAAGCCATCA GCGATTTGGC AGTGCGGCGG 
GCACGACACA GGTTGCTCTC TGGGGAATCA GGGGAAAAAC GTACCTCGAG ACCTGTCAAT 
AAAGTGATTA AATCGGCATC CGCTACTGCC TTGTCTCTCC TGATTCCCTC AGATCACCAC 
ACGTGTTCTC CATTGGCCAG TCCAATGTCA CCTCATTCTC TATCCTCCAA CCCATCTTCG 
AGGGACTCCT CACCCAGCCG CGACTTTTCT CCTGCTATCG CAAACCTGAA ACCACCAATC 
ATCATCCATC GGGCTGGAAA GAAATACGGT TTCACTCTCC GTGCCATTCG CGTCTATATG 
GGTGACAGTG ATATCTACAC TGTGCATCAC ATGGTCTGGC ACGTGGAGGA AGGTGGTCCA 
GCAAACGAAG CAGGTCTGCG TGAAGGGGAT CTGATAACCC ATGTCAACGG GGAGCCGGTC 
CATGGACTGG TGCACACAGA AGTGGTGGAA CTGATTCTGA AGAGTGGAAA TAAAGTGTCC 
ATCTCCACCA CGCCGTTTGA GAACACCTCC ATCAAAGTTG GTCCAGCTCG AAAAGCCAGC 
TACAAATCCA AGATGGCTCG TAGGAACAAG AAGAGCAAAA CTAAGGATGG TCAGGAAAGT 
AAGAAGAAGA GCTCTTTGTT GAGGAAGATC ACTAAACAGG CATCACTACT TCACACCAGC 
CGGAGTTTAT CTTCACTAAA CCGCTCTCTG TCTTCTGGAG AAAGCGTGCC TGGCTCTCCA 
ACTCACAACC TGTCTCCTCG GTCACCAACC CAGAGCTACA GATCCACTCC CGAGTCTGTA 
CACTCAGTTG GTGGCAATTC TTCCCAGAGC AGCTCTCCCA GTTCCAGTGT TCCCAATTCT 
CCAGCCAGCT CTGGACACAT CCGACCCAGC TCTCTGCACG GATTAGCACC GAAGCTTCAA 
AGGCAGTACA GGTCTCCGAG GCGCAAATCT GCGGGGAACA TCCCTCTGTC ACCCCTGGCT 
CACACTCCCT CACCCACCCC ACAGTCCACC TCACCGCAGC GCTCCCCATC TCCTTTACCA 
GGACACTCGG TTGGCAGCTC CAGTATCATC CAGTCTTTTC CAGTAAAACT ACACTCCTCT 
CCACCGCTGG TGAGACAAAT TTCTCGCCCC AAAAGCGCTG AGCCCCCTCG GTCTCCTTTA 
CTAAAGAGAG TGCAGTCAGC TGAGAAGCTG GCTGCCTCGC TGTCTTCTTC TGAGAAGAAG 
CTGGGCTCCT CGCGTAAGCA CAGCCTGGAT ATCTCTCACT CAGAATTTAA GAAGGAGATG 
CTCCAGAGGG ATCCCAGTCT CCAGAGTTTA CAAGAATCCG CGAATGAAAC AACGGGTGGC 
AAACTTGGGC TGGCAGAAAA AGGGATGTTG CAGAAGCCGG GTTCACGGAA GTTGGGTGCT 
ATTCGACAGG ACAGGGTGGA GAGGAGGGAG TCTCTGCAAA AGCAAGAGGC CATCAGGGAA 
GTAGATTCTT CCGAAGATGA AACGGATGAT GGTTCAGAAG ATAGTCAGGA TGGGAGAAGA 
CTAGACAAGC CCCCTGACAG CGGAGACCAA GGCTCAGATT CTTTTAATGA GGATAATAAG 
TTTTCATCTA AATTGGAAAG CAAGGATAGT ATCGAAGATG ATGCCTTTCT ACCTGAAGAT 
CCAAAAGGTA CGGAAGATTT GCAGAAGGGA AACAGTCAAC AAGCCAAGAC TATTTCAGAG 
CCGCTGCAAA TCAGTGTGCA CCCTTCCCCA TCAGAGGTCC CCCCAAGAAC TTCTCCAGAA 
AAATTAGGTA ATTCAGAAAA GCCATTCCTG AGCTCAGAAA CAACTGCAAA GGAACATAAA 
TTGCCAGAAA ACAAAACCTT TGAGTGTCTT GGTCCAAAAG ACAAGTCTTC TGAAGCAATC 
AAAGACCTAG GGAGAACTGC AAGTACCCAA AAACCAGTAG ACCGCACAGA GCATGTACAG 
TGCCCGTCTA TCAAACTCGT GGAACTTGAA GAAGGTGACT CTTTGGGGGC CTCGTGTGGT 
AAGCTTGACC TGAAAGACCC TCTGCTAACA GACAGCAGTC AGAAAAAACA GGATTCCAAA 
CCTCTGCTGG CACTTTCTTC GTGGTGCACG GCGAGCGTGA GCCCTCCCCT CTCGGTGAGC 
CCTGCGGATC GCAGTGAGAA GGATCACAAG GAGCCGCTTC AGCCTGCAGA ACAGCACAGC 
ACCTCCTTTC TGCCTCCTGG AAGCGTGAGT GAAACCTCCC AAAAAAGTCC TAGTACTGAA 
AAGCAGCCCA GCTCGTCCCA GGCGGAGAGC GCTTCGGCCG CCAGCAAAGC GACCAGCCCG 
GCTGCTCCTG TCCCCTCCAC GCTCCTCGTC CCCGCTGCCA TCGAAAGAGC TCTCTCCGTG 
TCCCAGTTAG TGCCACTCGC TCAGAGCGTG TTAGGCCCTT TGAAGCTCAG TATGTCTGGT 
TCCGGAGAGG TGGAAAAGAG AAAGGATTTC CCCCATTTGG GTGCCTCCTG TAAAGAAGAG 
AGAGATTCCA AACAAAAAAA GCAGGAAACT TCTCAAGGAG GAGGAGCGTG TCAAGAGAAA 
GCCAAACTAT CTAGCACAGG CGGTCTGGCC ACCAGGAGCA GGTCCTGCAC AGAGTCGTTA 
AACTCGGCCA CGTGTCCCGT GGTGGCAAAA AAGGAGGCAG CTTTTTGCAG CCCTAAAGCA 
TCTGAGGTGT TGGGAGGTAG CTGCAAAACC ACACCATCGA AAGAGCTGTC TCACGCTCTT 
GGACAAGGAG CTCTGAGAGC CTCTCCTCTG CCAGATGGCA GCACGAGGCC CTCTAAAGAA 
GGGACTCTGG CGCAGGCAAA AAGCAAAGAG GTTGGAAATC AGTTAAAAAT ACAAGACTTT 
CCCCTGTCAC ATGATGGTGC TGTAAAGAAA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794677.1
CDS	4..5979
Translation	MITESTPLRCRKLSNPDIFSSAGKTKLHRQLSQDDCKLRRGSLASSLSGKQLLPLSNSVHSGVGQTIWQPPGDTSNLVRMRNQSLGQSAPSLTAGLSGIPAKRDSMLLFFYKIFLLVNFIYRATSLPIHLNPSLLKANTDNMISEVILLWKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHAGSSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTESITDEEGRHSPALRPRSRSLSPGRSPISFDSEIIMMNHVYKERFPKATAQMEERLAEFIASNAPENVLQLADGVLSFIHHQVIELARDCLDKSREGLITSRYFYELQENLEKLLQDAHERSESSEVAFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIISQLGLTRDPLEEMAQLNSYDSPDTPETDDSVESRGASVQSKKTPSEEEFETIKLISNGAYGAVYLVRHKTTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVISDEIAWPEGDDALPPDAQDLISKLLRQNPLERMGTGSAFEVKQHRFFKDLDWNGLLRQKAEFIPQLESEDDTSYFDTRSERYQHLDSEEEEDTNDDDHVEIRQFSSCSPRFSKVYSSMERLSIHEERKTPPPTKRSLSEEKDDRLDSLGGLKSRDRSWVIGSPEILRKRLSMSESSHTESDSSPPLTVRRRCSGLLDMPRFAISTEDEGTVLKRPQSEGMLLSTVQSREGLPVPIPEQPVEQELQLEGDAGPTTPSTAVSSASTLTAGSTADFSDPRARSNSNEGPDFTTPKAISDLAVRRARHRLLSGESGEKRTSRPVNKVIKSASATALSLLIPSDHHTCSPLASPMSPHSLSSNPSSRDSSPSRDFSPAIANLKPPIIIHRAGKKYGFTLRAIRVYMGDSDIYTVHHMVWHVEEGGPANEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPFENTSIKVGPARKASYKSKMARRNKKSKTKDGQESKKKSSLLRKITKQASLLHTSRSLSSLNRSLSSGESVPGSPTHNLSPRSPTQSYRSTPESVHSVGGNSSQSSSPSSSVPNSPASSGHIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLAHTPSPTPQSTSPQRSPSPLPGHSVGSSSIIQSFPVKLHSSPPLVRQISRPKSAEPPRSPLLKRVQSAEKLAASLSSSEKKLGSSRKHSLDISHSEFKKEMLQRDPSLQSLQESANETTGGKLGLAEKGMLQKPGSRKLGAIRQDRVERRESLQKQEAIREVDSSEDETDDGSEDSQDGRRLDKPPDSGDQGSDSFNEDNKFSSKLESKDSIEDDAFLPEDPKGTEDLQKGNSQQAKTISEPLQISVHPSPSEVPPRTSPEKLGNSEKPFLSSETTAKEHKLPENKTFECLGPKDKSSEAIKDLGRTASTQKPVDRTEHVQCPSIKLVELEEGDSLGASCGKLDLKDPLLTDSSQKKQDSKPLLALSSWCTASVSPPLSVSPADRSEKDHKEPLQPAEQHSTSFLPPGSVSETSQKSPSTEKQPSSSQAESASAASKATSPAAPVPSTLLVPAAIERALSVSQLVPLAQSVLGPLKLSMSGSGEVEKRKDFPHLGASCKEERDSKQKKQETSQGGGACQEKAKLSSTGGLATRSRSCTESLNSATCPVVAKKEAAFCSPKASEVLGGSCKTTPSKELSHALGQGALRASPLPDGSTRPSKEGTLAQAKSKEVGNQLKIQDFPLSHDGAVKKT

Target ORF information:

RefSeq Version	XM_014939191.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939191.1

ORF Insert Sequence:

ATGATAACAG AGTCCACTCC ATTACGCTGC AGGAAGCTCA GCAATCCTGA CATCTTTTCA 
TCTGCTGGGA AAACCAAGCT CCATCGTCAG CTAAGTCAAG ATGACTGCAA GCTACGAAGA 
GGTAGTTTGG CAAGTTCTTT GTCAGGAAAA CAGCTACTTC CCTTATCAAA TAGTGTCCAC 
AGTGGAGTGG GACAAACAAT ATGGCAGCCA CCTGGAGATA CCTCAAACCT GGTCCGCATG 
AGAAATCAGT CCCTTGGACA ATCTGCTCCT TCACTTACTG CTGGTTTGTC TGGGATTCCT 
GCCAAAAGAG ATTCCATGTT GCTTTTCTTC TATAAAATTT TTCTCCTGGT AAACTTCATC 
TACAGAGCCA CTTCCCTGCC CATACATCTT AATCCATCAC TCCTGAAAGC AAATACTGAC 
AACATGATCT CTGAGGTGAT CCTCCTGTGG AAGGAGCTCA GTCTCCCCAG AAGAGGAAGC 
TTCTGTCGGA CAAGCAACAG AAAGAGTTTG ATCGTAACAT CCAGCACATC TCCAACTCTC 
CCGCGACCAC ATTCCCCTCT TCATGGACAC GCAGGTAGCA GCCCTTTGGA CAGTCCCAGG 
AACTTCTCTC CAAATGCACC TGCTCATTTT TCCTTTGTTC CTGCCCGAAG CCACGGACAC 
AGAGCAGACA GAACGGATGG GCGCCGCTGG TCCTTGGCCT CCCTTCCTTC ATCTGGATAT 
GGAACAAACA CTCCCAGTTC AACAGTTTCA TCATCATGCT CATCTCAGGA GAAGCTGCAC 
CAGCTGCCCT TTCAGCCCAC GGCAGATGAA CTTCATTTCC TGACAAAGCA TTTCAGTACT 
GAGAGCATCA CTGATGAGGA AGGGCGCCAT TCTCCAGCCT TGAGGCCTCG ATCTCGCAGT 
CTCAGCCCGG GTCGCTCCCC AATTTCCTTT GACAGTGAAA TAATAATGAT GAATCATGTG 
TACAAAGAAA GGTTTCCAAA GGCCACGGCG CAGATGGAAG AACGGCTGGC TGAGTTTATT 
GCCTCTAATG CTCCAGAGAA CGTGCTGCAA TTAGCAGATG GGGTCCTAAG TTTCATTCAT 
CATCAAGTTA TTGAACTGGC CAGAGATTGC CTAGATAAAT CACGTGAAGG TCTTATTACT 
TCTCGGTACT TTTATGAGCT GCAGGAAAAC TTGGAGAAAC TTCTCCAGGA TGCCCACGAG 
CGATCCGAGA GCTCCGAGGT TGCCTTCGTT ACCCAACTTG TGAAGAAGCT GATGATTATC 
ATTGCACGGC CGGCTCGGCT GCTGGAGTGC CTGGAATTTG ATCCTGAAGA GTTCTACCAT 
TTGCTAGAAG CTGCAGAAGG CCATGCCAAG GAAGGGCAAG GAATTAAATG TGACATTCCT 
CGTTATATTA TCAGCCAGTT GGGACTCACC AGGGATCCCC TGGAGGAAAT GGCCCAGCTG 
AATAGCTATG ACAGCCCAGA CACTCCTGAG ACAGATGATT CAGTCGAGAG CCGTGGGGCC 
TCTGTCCAGT CAAAGAAAAC TCCATCTGAA GAAGAGTTTG AAACCATTAA ACTGATCAGT 
AATGGTGCTT ATGGAGCGGT GTATTTGGTG CGACACAAGA CCACCCGTCA ACGTTTTGCC 
ATGAAGAAGA TCAACAAACA GAACCTAATT TTGAGAAACC AGATCCAGCA GGCTTTTGTG 
GAGAGAGATA TCTTGACGTT TGCTGAGAAC CCCTTCGTGG TCAGCATGTT CTGCTCCTTT 
GAGACCAAGC GGCACCTGTG CATGGTTATG GAGTATGTGG AAGGAGGTGA TTGTGCCACC 
CTTCTCAAGA ACATCGGTGC CCTGCCTGTT GATATGGCGA GGATGTATTT TGCTGAGACT 
GTTCTGGCAT TGGAGTACCT ACACAATTAT GGGATTGTTC ACCGTGACCT AAAACCTGAT 
AATCTCCTGA TAACTTCCAT GGGTCACATT AAACTAACAG ACTTTGGACT GTCTAAAATT 
GGGTTAATGA GTCTTACAAC TAACCTTTAT GAGGGACACA TTGAGAAGGA TACCAGGGAG 
TTCCTGGACA AGCAGGTCTG TGGGACTCCA GAATACATCG CGCCAGAAGT GATCCTGCGC 
CAGGGCTATG GGAAGCCCGT GGACTGGTGG GCCATGGGAG TTATCTTGTA CGAGTTTCTG 
GTCGGCTGCG TCCCTTTCTT TGGGGACACA CCTGAAGAGC TGTTTGGACA AGTGATCAGT 
GATGAAATTG CCTGGCCAGA AGGTGATGAT GCACTGCCGC CAGATGCCCA GGACCTCATT 
TCTAAGCTTC TCCGCCAAAA TCCTCTGGAA AGAATGGGAA CAGGTAGTGC CTTTGAAGTG 
AAACAGCATC GGTTCTTTAA AGATTTGGAC TGGAATGGAT TACTTCGGCA GAAAGCTGAA 
TTCATCCCAC AGTTGGAATC TGAGGATGAC ACAAGCTATT TCGACACCCG TTCAGAACGG 
TACCAACACC TGGATTCAGA AGAAGAGGAG GACACTAATG ATGATGATCA TGTGGAAATT 
CGGCAATTTT CCTCATGCTC ACCAAGGTTC AGCAAGGTAT ACAGCAGCAT GGAACGTCTT 
TCCATCCACG AAGAGAGGAA GACTCCACCA CCAACTAAAC GGAGTCTGAG TGAAGAAAAA 
GATGACCGGC TGGACAGCCT TGGTGGCTTG AAGAGTCGAG ACCGCAGCTG GGTGATTGGG 
TCTCCTGAAA TACTGCGGAA GCGCTTGTCT ATGTCTGAAT CCTCGCACAC GGAGAGTGAC 
TCCAGCCCAC CCTTGACCGT CCGCCGGCGC TGCTCAGGGC TCCTCGATAT GCCCCGTTTT 
GCCATCTCCA CTGAGGACGA GGGAACTGTT CTCAAAAGGC CACAGTCCGA GGGGATGCTC 
TTATCCACCG TGCAGTCCCG GGAGGGGCTG CCAGTGCCCA TTCCAGAGCA GCCTGTGGAG 
CAAGAGCTGC AGCTGGAAGG TGATGCTGGA CCCACCACCC CCTCCACAGC AGTCAGCAGC 
GCCTCAACGC TGACAGCTGG GAGCACTGCA GATTTCTCTG ATCCACGCGC TCGCAGTAAT 
AGCAATGAAG GCCCAGACTT TACCACTCCA AAAGCCATCA GCGATTTGGC AGTGCGGCGG 
GCACGACACA GGTTGCTCTC TGGGGAATCA GGGGAAAAAC GTACCTCGAG ACCTGTCAAT 
AAAGTGATTA AATCGGCATC CGCTACTGCC TTGTCTCTCC TGATTCCCTC AGATCACCAC 
ACGTGTTCTC CATTGGCCAG TCCAATGTCA CCTCATTCTC TATCCTCCAA CCCATCTTCG 
AGGGACTCCT CACCCAGCCG CGACTTTTCT CCTGCTATCG CAAACCTGAA ACCACCAATC 
ATCATCCATC GGGCTGGAAA GAAATACGGT TTCACTCTCC GTGCCATTCG CGTCTATATG 
GGTGACAGTG ATATCTACAC TGTGCATCAC ATGGTCTGGC ACGTGGAGGA AGGTGGTCCA 
GCAAACGAAG CAGGTCTGCG TGAAGGGGAT CTGATAACCC ATGTCAACGG GGAGCCGGTC 
CATGGACTGG TGCACACAGA AGTGGTGGAA CTGATTCTGA AGAGTGGAAA TAAAGTGTCC 
ATCTCCACCA CGCCGTTTGA GAACACCTCC ATCAAAGTTG GTCCAGCTCG AAAAGCCAGC 
TACAAATCCA AGATGGCTCG TAGGAACAAG AAGAGCAAAA CTAAGGATGG TCAGGAAAGT 
AAGAAGAAGA GCTCTTTGTT GAGGAAGATC ACTAAACAGG CATCACTACT TCACACCAGC 
CGGAGTTTAT CTTCACTAAA CCGCTCTCTG TCTTCTGGAG AAAGCGTGCC TGGCTCTCCA 
ACTCACAACC TGTCTCCTCG GTCACCAACC CAGAGCTACA GATCCACTCC CGAGTCTGTA 
CACTCAGTTG GTGGCAATTC TTCCCAGAGC AGCTCTCCCA GTTCCAGTGT TCCCAATTCT 
CCAGCCAGCT CTGGACACAT CCGACCCAGC TCTCTGCACG GATTAGCACC GAAGCTTCAA 
AGGCAGTACA GGTCTCCGAG GCGCAAATCT GCGGGGAACA TCCCTCTGTC ACCCCTGGCT 
CACACTCCCT CACCCACCCC ACAGTCCACC TCACCGCAGC GCTCCCCATC TCCTTTACCA 
GGACACTCGG TTGGCAGCTC CAGTATCATC CAGTCTTTTC CAGTAAAACT ACACTCCTCT 
CCACCGCTGG TGAGACAAAT TTCTCGCCCC AAAAGCGCTG AGCCCCCTCG GTCTCCTTTA 
CTAAAGAGAG TGCAGTCAGC TGAGAAGCTG GCTGCCTCGC TGTCTTCTTC TGAGAAGAAG 
CTGGGCTCCT CGCGTAAGCA CAGCCTGGAT ATCTCTCACT CAGAATTTAA GAAGGAGATG 
CTCCAGAGGG ATCCCAGTCT CCAGAGTTTA CAAGAATCCG CGAATGAAAC AACGGGTGGC 
AAACTTGGGC TGGCAGAAAA AGGGATGTTG CAGAAGCCGG GTTCACGGAA GTTGGGTGCT 
ATTCGACAGG ACAGGGTGGA GAGGAGGGAG TCTCTGCAAA AGCAAGAGGC CATCAGGGAA 
GTAGATTCTT CCGAAGATGA AACGGATGAT GGTTCAGAAG ATAGTCAGGA TGGGAGAAGA 
CTAGACAAGC CCCCTGACAG CGGAGACCAA GGCTCAGATT CTTTTAATGA GGATAATAAG 
TTTTCATCTA AATTGGAAAG CAAGGATAGT ATCGAAGATG ATGCCTTTCT ACCTGAAGAT 
CCAAAAGGTA CGGAAGATTT GCAGAAGGGA AACAGTCAAC AAGCCAAGAC TATTTCAGAG 
CCGCTGCAAA TCAGTGTGCA CCCTTCCCCA TCAGAGGTCC CCCCAAGAAC TTCTCCAGAA 
AAATTAGGTA ATTCAGAAAA GCCATTCCTG AGCTCAGAAA CAACTGCAAA GGAACATAAA 
TTGCCAGAAA ACAAAACCTT TGAGTGTCTT GGTCCAAAAG ACAAGTCTTC TGAAGCAATC 
AAAGACCTAG GGAGAACTGC AAGTACCCAA AAACCAGTAG ACCGCACAGA GCATGTACAG 
TGCCCGTCTA TCAAACTCGT GGAACTTGAA GAAGGTGACT CTTTGGGGGC CTCGTGTGGT 
AAGCTTGACC TGAAAGACCC TCTGCTAACA GACAGCAGTC AGAAAAAACA GGATTCCAAA 
CCTCTGCTGG CACTTTCTTC GTGGTGCACG GCGAGCGTGA GCCCTCCCCT CTCGGTGAGC 
CCTGCGGATC GCAGTGAGAA GGATCACAAG GAGCCGCTTC AGCCTGCAGA ACAGCACAGC 
ACCTCCTTTC TGCCTCCTGG AAGCGTGAGT GAAACCTCCC AAAAAAGTCC TAGTACTGAA 
AAGCAGCCCA GCTCGTCCCA GGCGGAGAGC GCTTCGGCCG CCAGCAAAGC GACCAGCCCG 
GCTGCTCCTG TCCCCTCCAC GCTCCTCGTC CCCGCTGCCA TCGAAAGAGC TCTCTCCGTG 
TCCCAGTTAG TGCCACTCGC TCAGAGCGTG TTAGGCCCTT TGAAGCTCAG TATGTCTGGT 
TCCGGAGAGG TGGAAAAGAG AAAGGATTTC CCCCATTTGG GTGCCTCCTG TAAAGAAGAG 
AGAGATTCCA AACAAAAAAA GCAGGAAACT TCTCAAGGAG GAGGAGCGTG TCAAGAGAAA 
GCCAAACTAT CTAGCACAGG CGGTCTGGCC ACCAGGAGCA GGTCCTGCAC AGAGTCGTTA 
AACTCGGCCA CGTGTCCCGT GGTGGCAAAA AAGGAGGCAG CTTTTTGCAG CCCTAAAGCA 
TCTGAGGTGT TGGGAGGTAG CTGCAAAACC ACACCATCGA AAGAGCTGTC TCACGCTCTT 
GGACAAGGAG CTCTGAGAGC CTCTCCTCTG CCAGATGGCA GCACGAGGCC CTCTAAAGAA 
GGGACTCTGG CGCAGGCAAA AAGCAAAGAG GTTGGAAATC AGTTAAAAAT ACAAGACTTT 
CCCCTGTCAC ATGATGGTGC TGTAAAGAAA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_014939192.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794678.1 Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

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CloneID	OCa62992
Clone ID Related Accession (Same CDS sequence)	XM_014939192.1
Accession Version	XM_014939192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090812.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAACAG AGTCCACTCC ATTACGCTGC AGGAAGCTCA GCAATCCTGA CATCTTTTCA 
TCTGCTGGGA AAACCAAGCT CCATCGTCAG CTAAGTCAAG ATGACTGCAA GCTACGAAGA 
GGTAGTTTGG CAAGTTCTTT GTCAGGAAAA CAGCTACTTC CCTTATCAAA TAGTGTCCAC 
AGTGGAGTGG GACAAACAAT ATGGCAGCCA CCTGGAGATA CCTCAAACCT GGTCCGCATG 
AGAAATCAGT CCCTTGGACA ATCTGCTCCT TCACTTACTG CTGGTTTGTC TGGGATTCCT 
GCCAAAAGAG ATTCCATGTT GCTTTTCTTC TATAAAATTT TTCTCCTGGT AAACTTCATC 
TACAGAGCCA CTTCCCTGCC CATACATCTT AATCCATCAC TCCTGAAAGC AAATACTGAC 
AACATGATCT CTGAGGTGAT CCTCCTGTGG AAGGAGCTCA GTCTCCCCAG AAGAGGAAGC 
TTCTGTCGGA CAAGCAACAG AAAGAGTTTG ATCGTAACAT CCAGCACATC TCCAACTCTC 
CCGCGACCAC ATTCCCCTCT TCATGGACAC GCAGGTAGCA GCCCTTTGGA CAGTCCCAGG 
AACTTCTCTC CAAATGCACC TGCTCATTTT TCCTTTGTTC CTGCCCGAAG AACGGATGGG 
CGCCGCTGGT CCTTGGCCTC CCTTCCTTCA TCTGGATATG GAACAAACAC TCCCAGTTCA 
ACAGTTTCAT CATCATGCTC ATCTCAGGAG AAGCTGCACC AGCTGCCCTT TCAGCCCACG 
GCAGATGAAC TTCATTTCCT GACAAAGCAT TTCAGTACTG AGAGCATCAC TGATGAGGAA 
GGGCGCCATT CTCCAGCCTT GAGGCCTCGA TCTCGCAGTC TCAGCCCGGG TCGCTCCCCA 
ATTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTGT ACAAAGAAAG GTTTCCAAAG 
GCCACGGCGC AGATGGAAGA ACGGCTGGCT GAGTTTATTG CCTCTAATGC TCCAGAGAAC 
GTGCTGCAAT TAGCAGATGG GGTCCTAAGT TTCATTCATC ATCAAGTTAT TGAACTGGCC 
AGAGATTGCC TAGATAAATC ACGTGAAGGT CTTATTACTT CTCGGTACTT TTATGAGCTG 
CAGGAAAACT TGGAGAAACT TCTCCAGGAT GCCCACGAGC GATCCGAGAG CTCCGAGGTT 
GCCTTCGTTA CCCAACTTGT GAAGAAGCTG ATGATTATCA TTGCACGGCC GGCTCGGCTG 
CTGGAGTGCC TGGAATTTGA TCCTGAAGAG TTCTACCATT TGCTAGAAGC TGCAGAAGGC 
CATGCCAAGG AAGGGCAAGG AATTAAATGT GACATTCCTC GTTATATTAT CAGCCAGTTG 
GGACTCACCA GGGATCCCCT GGAGGAAATG GCCCAGCTGA ATAGCTATGA CAGCCCAGAC 
ACTCCTGAGA CAGATGATTC AGTCGAGAGC CGTGGGGCCT CTGTCCAGTC AAAGAAAACT 
CCATCTGAAG AAGAGTTTGA AACCATTAAA CTGATCAGTA ATGGTGCTTA TGGAGCGGTG 
TATTTGGTGC GACACAAGAC CACCCGTCAA CGTTTTGCCA TGAAGAAGAT CAACAAACAG 
AACCTAATTT TGAGAAACCA GATCCAGCAG GCTTTTGTGG AGAGAGATAT CTTGACGTTT 
GCTGAGAACC CCTTCGTGGT CAGCATGTTC TGCTCCTTTG AGACCAAGCG GCACCTGTGC 
ATGGTTATGG AGTATGTGGA AGGAGGTGAT TGTGCCACCC TTCTCAAGAA CATCGGTGCC 
CTGCCTGTTG ATATGGCGAG GATGTATTTT GCTGAGACTG TTCTGGCATT GGAGTACCTA 
CACAATTATG GGATTGTTCA CCGTGACCTA AAACCTGATA ATCTCCTGAT AACTTCCATG 
GGTCACATTA AACTAACAGA CTTTGGACTG TCTAAAATTG GGTTAATGAG TCTTACAACT 
AACCTTTATG AGGGACACAT TGAGAAGGAT ACCAGGGAGT TCCTGGACAA GCAGGTCTGT 
GGGACTCCAG AATACATCGC GCCAGAAGTG ATCCTGCGCC AGGGCTATGG GAAGCCCGTG 
GACTGGTGGG CCATGGGAGT TATCTTGTAC GAGTTTCTGG TCGGCTGCGT CCCTTTCTTT 
GGGGACACAC CTGAAGAGCT GTTTGGACAA GTGATCAGTG ATGAAATTGC CTGGCCAGAA 
GGTGATGATG CACTGCCGCC AGATGCCCAG GACCTCATTT CTAAGCTTCT CCGCCAAAAT 
CCTCTGGAAA GAATGGGAAC AGGTAGTGCC TTTGAAGTGA AACAGCATCG GTTCTTTAAA 
GATTTGGACT GGAATGGATT ACTTCGGCAG AAAGCTGAAT TCATCCCACA GTTGGAATCT 
GAGGATGACA CAAGCTATTT CGACACCCGT TCAGAACGGT ACCAACACCT GGATTCAGAA 
GAAGAGGAGG ACACTAATGA TGATGATCAT GTGGAAATTC GGCAATTTTC CTCATGCTCA 
CCAAGGTTCA GCAAGGTATA CAGCAGCATG GAACGTCTTT CCATCCACGA AGAGAGGAAG 
ACTCCACCAC CAACTAAACG GAGTCTGAGT GAAGAAAAAG ATGACCGGCT GGACAGCCTT 
GGTGGCTTGA AGAGTCGAGA CCGCAGCTGG GTGATTGGGT CTCCTGAAAT ACTGCGGAAG 
CGCTTGTCTA TGTCTGAATC CTCGCACACG GAGAGTGACT CCAGCCCACC CTTGACCGTC 
CGCCGGCGCT GCTCAGGGCT CCTCGATATG CCCCGTTTTG CCATCTCCAC TGAGGACGAG 
GGAACTGTTC TCAAAAGGCC ACAGTCCGAG GGGATGCTCT TATCCACCGT GCAGTCCCGG 
GAGGGGCTGC CAGTGCCCAT TCCAGAGCAG CCTGTGGAGC AAGAGCTGCA GCTGGAAGGT 
GATGCTGGAC CCACCACCCC CTCCACAGCA GTCAGCAGCG CCTCAACGCT GACAGCTGGG 
AGCACTGCAG ATTTCTCTGA TCCACGCGCT CGCAGTAATA GCAATGAAGG CCCAGACTTT 
ACCACTCCAA AAGCCATCAG CGATTTGGCA GTGCGGCGGG CACGACACAG GTTGCTCTCT 
GGGGAATCAG GGGAAAAACG TACCTCGAGA CCTGTCAATA AAGTGATTAA ATCGGCATCC 
GCTACTGCCT TGTCTCTCCT GATTCCCTCA GATCACCACA CGTGTTCTCC ATTGGCCAGT 
CCAATGTCAC CTCATTCTCT ATCCTCCAAC CCATCTTCGA GGGACTCCTC ACCCAGCCGC 
GACTTTTCTC CTGCTATCGC AAACCTGAAA CCACCAATCA TCATCCATCG GGCTGGAAAG 
AAATACGGTT TCACTCTCCG TGCCATTCGC GTCTATATGG GTGACAGTGA TATCTACACT 
GTGCATCACA TGGTCTGGCA CGTGGAGGAA GGTGGTCCAG CAAACGAAGC AGGTCTGCGT 
GAAGGGGATC TGATAACCCA TGTCAACGGG GAGCCGGTCC ATGGACTGGT GCACACAGAA 
GTGGTGGAAC TGATTCTGAA GAGTGGAAAT AAAGTGTCCA TCTCCACCAC GCCGTTTGAG 
AACACCTCCA TCAAAGTTGG TCCAGCTCGA AAAGCCAGCT ACAAATCCAA GATGGCTCGT 
AGGAACAAGA AGAGCAAAAC TAAGGATGGT CAGGAAAGTA AGAAGAAGAG CTCTTTGTTG 
AGGAAGATCA CTAAACAGGC ATCACTACTT CACACCAGCC GGAGTTTATC TTCACTAAAC 
CGCTCTCTGT CTTCTGGAGA AAGCGTGCCT GGCTCTCCAA CTCACAACCT GTCTCCTCGG 
TCACCAACCC AGAGCTACAG ATCCACTCCC GAGTCTGTAC ACTCAGTTGG TGGCAATTCT 
TCCCAGAGCA GCTCTCCCAG TTCCAGTGTT CCCAATTCTC CAGCCAGCTC TGGACACATC 
CGACCCAGCT CTCTGCACGG ATTAGCACCG AAGCTTCAAA GGCAGTACAG GTCTCCGAGG 
CGCAAATCTG CGGGGAACAT CCCTCTGTCA CCCCTGGCTC ACACTCCCTC ACCCACCCCA 
CAGTCCACCT CACCGCAGCG CTCCCCATCT CCTTTACCAG GACACTCGGT TGGCAGCTCC 
AGTATCATCC AGTCTTTTCC AGTAAAACTA CACTCCTCTC CACCGCTGGT GAGACAAATT 
TCTCGCCCCA AAAGCGCTGA GCCCCCTCGG TCTCCTTTAC TAAAGAGAGT GCAGTCAGCT 
GAGAAGCTGG CTGCCTCGCT GTCTTCTTCT GAGAAGAAGC TGGGCTCCTC GCGTAAGCAC 
AGCCTGGATA TCTCTCACTC AGAATTTAAG AAGGAGATGC TCCAGAGGGA TCCCAGTCTC 
CAGAGTTTAC AAGAATCCGC GAATGAAACA ACGGGTGGCA AACTTGGGCT GGCAGAAAAA 
GGGATGTTGC AGAAGCCGGG TTCACGGAAG TTGGGTGCTA TTCGACAGGA CAGGGTGGAG 
AGGAGGGAGT CTCTGCAAAA GCAAGAGGCC ATCAGGGAAG TAGATTCTTC CGAAGATGAA 
ACGGATGATG GTTCAGAAGA TAGTCAGGAT GGGAGAAGAC TAGACAAGCC CCCTGACAGC 
GGAGACCAAG GCTCAGATTC TTTTAATGAG GATAATAAGT TTTCATCTAA ATTGGAAAGC 
AAGGATAGTA TCGAAGATGA TGCCTTTCTA CCTGAAGATC CAAAAGGTAC GGAAGATTTG 
CAGAAGGGAA ACAGTCAACA AGCCAAGACT ATTTCAGAGC CGCTGCAAAT CAGTGTGCAC 
CCTTCCCCAT CAGAGGTCCC CCCAAGAACT TCTCCAGAAA AATTAGGTAA TTCAGAAAAG 
CCATTCCTGA GCTCAGAAAC AACTGCAAAG GAACATAAAT TGCCAGAAAA CAAAACCTTT 
GAGTGTCTTG GTCCAAAAGA CAAGTCTTCT GAAGCAATCA AAGACCTAGG GAGAACTGCA 
AGTACCCAAA AACCAGTAGA CCGCACAGAG CATGTACAGT GCCCGTCTAT CAAACTCGTG 
GAACTTGAAG AAGGTGACTC TTTGGGGGCC TCGTGTGGTA AGCTTGACCT GAAAGACCCT 
CTGCTAACAG ACAGCAGTCA GAAAAAACAG GATTCCAAAC CTCTGCTGGC ACTTTCTTCG 
TGGTGCACGG CGAGCGTGAG CCCTCCCCTC TCGGTGAGCC CTGCGGATCG CAGTGAGAAG 
GATCACAAGG AGCCGCTTCA GCCTGCAGAA CAGCACAGCA CCTCCTTTCT GCCTCCTGGA 
AGCGTGAGTG AAACCTCCCA AAAAAGTCCT AGTACTGAAA AGCAGCCCAG CTCGTCCCAG 
GCGGAGAGCG CTTCGGCCGC CAGCAAAGCG ACCAGCCCGG CTGCTCCTGT CCCCTCCACG 
CTCCTCGTCC CCGCTGCCAT CGAAAGAGCT CTCTCCGTGT CCCAGTTAGT GCCACTCGCT 
CAGAGCGTGT TAGGCCCTTT GAAGCTCAGT ATGTCTGGTT CCGGAGAGGT GGAAAAGAGA 
AAGGATTTCC CCCATTTGGG TGCCTCCTGT AAAGAAGAGA GAGATTCCAA ACAAAAAAAG 
CAGGAAACTT CTCAAGGAGG AGGAGCGTGT CAAGAGAAAG CCAAACTATC TAGCACAGGC 
GGTCTGGCCA CCAGGAGCAG GTCCTGCACA GAGTCGTTAA ACTCGGCCAC GTGTCCCGTG 
GTGGCAAAAA AGGAGGCAGC TTTTTGCAGC CCTAAAGCAT CTGAGGTGTT GGGAGGTAGC 
TGCAAAACCA CACCATCGAA AGAGCTGTCT CACGCTCTTG GACAAGGAGC TCTGAGAGCC 
TCTCCTCTGC CAGATGGCAG CACGAGGCCC TCTAAAGAAG GGACTCTGGC GCAGGCAAAA 
AGCAAAGAGG TTGGAAATCA GTTAAAAATA CAAGACTTTC CCCTGTCACA TGATGGTGCT 
GTAAAGAAAA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794678.1
CDS	4..5958
Translation	MITESTPLRCRKLSNPDIFSSAGKTKLHRQLSQDDCKLRRGSLASSLSGKQLLPLSNSVHSGVGQTIWQPPGDTSNLVRMRNQSLGQSAPSLTAGLSGIPAKRDSMLLFFYKIFLLVNFIYRATSLPIHLNPSLLKANTDNMISEVILLWKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHAGSSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTESITDEEGRHSPALRPRSRSLSPGRSPISFDSEIIMMNHVYKERFPKATAQMEERLAEFIASNAPENVLQLADGVLSFIHHQVIELARDCLDKSREGLITSRYFYELQENLEKLLQDAHERSESSEVAFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIISQLGLTRDPLEEMAQLNSYDSPDTPETDDSVESRGASVQSKKTPSEEEFETIKLISNGAYGAVYLVRHKTTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVISDEIAWPEGDDALPPDAQDLISKLLRQNPLERMGTGSAFEVKQHRFFKDLDWNGLLRQKAEFIPQLESEDDTSYFDTRSERYQHLDSEEEEDTNDDDHVEIRQFSSCSPRFSKVYSSMERLSIHEERKTPPPTKRSLSEEKDDRLDSLGGLKSRDRSWVIGSPEILRKRLSMSESSHTESDSSPPLTVRRRCSGLLDMPRFAISTEDEGTVLKRPQSEGMLLSTVQSREGLPVPIPEQPVEQELQLEGDAGPTTPSTAVSSASTLTAGSTADFSDPRARSNSNEGPDFTTPKAISDLAVRRARHRLLSGESGEKRTSRPVNKVIKSASATALSLLIPSDHHTCSPLASPMSPHSLSSNPSSRDSSPSRDFSPAIANLKPPIIIHRAGKKYGFTLRAIRVYMGDSDIYTVHHMVWHVEEGGPANEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPFENTSIKVGPARKASYKSKMARRNKKSKTKDGQESKKKSSLLRKITKQASLLHTSRSLSSLNRSLSSGESVPGSPTHNLSPRSPTQSYRSTPESVHSVGGNSSQSSSPSSSVPNSPASSGHIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLAHTPSPTPQSTSPQRSPSPLPGHSVGSSSIIQSFPVKLHSSPPLVRQISRPKSAEPPRSPLLKRVQSAEKLAASLSSSEKKLGSSRKHSLDISHSEFKKEMLQRDPSLQSLQESANETTGGKLGLAEKGMLQKPGSRKLGAIRQDRVERRESLQKQEAIREVDSSEDETDDGSEDSQDGRRLDKPPDSGDQGSDSFNEDNKFSSKLESKDSIEDDAFLPEDPKGTEDLQKGNSQQAKTISEPLQISVHPSPSEVPPRTSPEKLGNSEKPFLSSETTAKEHKLPENKTFECLGPKDKSSEAIKDLGRTASTQKPVDRTEHVQCPSIKLVELEEGDSLGASCGKLDLKDPLLTDSSQKKQDSKPLLALSSWCTASVSPPLSVSPADRSEKDHKEPLQPAEQHSTSFLPPGSVSETSQKSPSTEKQPSSSQAESASAASKATSPAAPVPSTLLVPAAIERALSVSQLVPLAQSVLGPLKLSMSGSGEVEKRKDFPHLGASCKEERDSKQKKQETSQGGGACQEKAKLSSTGGLATRSRSCTESLNSATCPVVAKKEAAFCSPKASEVLGGSCKTTPSKELSHALGQGALRASPLPDGSTRPSKEGTLAQAKSKEVGNQLKIQDFPLSHDGAVKKT

Target ORF information:

RefSeq Version	XM_014939192.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939192.1

ORF Insert Sequence:

ATGATAACAG AGTCCACTCC ATTACGCTGC AGGAAGCTCA GCAATCCTGA CATCTTTTCA 
TCTGCTGGGA AAACCAAGCT CCATCGTCAG CTAAGTCAAG ATGACTGCAA GCTACGAAGA 
GGTAGTTTGG CAAGTTCTTT GTCAGGAAAA CAGCTACTTC CCTTATCAAA TAGTGTCCAC 
AGTGGAGTGG GACAAACAAT ATGGCAGCCA CCTGGAGATA CCTCAAACCT GGTCCGCATG 
AGAAATCAGT CCCTTGGACA ATCTGCTCCT TCACTTACTG CTGGTTTGTC TGGGATTCCT 
GCCAAAAGAG ATTCCATGTT GCTTTTCTTC TATAAAATTT TTCTCCTGGT AAACTTCATC 
TACAGAGCCA CTTCCCTGCC CATACATCTT AATCCATCAC TCCTGAAAGC AAATACTGAC 
AACATGATCT CTGAGGTGAT CCTCCTGTGG AAGGAGCTCA GTCTCCCCAG AAGAGGAAGC 
TTCTGTCGGA CAAGCAACAG AAAGAGTTTG ATCGTAACAT CCAGCACATC TCCAACTCTC 
CCGCGACCAC ATTCCCCTCT TCATGGACAC GCAGGTAGCA GCCCTTTGGA CAGTCCCAGG 
AACTTCTCTC CAAATGCACC TGCTCATTTT TCCTTTGTTC CTGCCCGAAG AACGGATGGG 
CGCCGCTGGT CCTTGGCCTC CCTTCCTTCA TCTGGATATG GAACAAACAC TCCCAGTTCA 
ACAGTTTCAT CATCATGCTC ATCTCAGGAG AAGCTGCACC AGCTGCCCTT TCAGCCCACG 
GCAGATGAAC TTCATTTCCT GACAAAGCAT TTCAGTACTG AGAGCATCAC TGATGAGGAA 
GGGCGCCATT CTCCAGCCTT GAGGCCTCGA TCTCGCAGTC TCAGCCCGGG TCGCTCCCCA 
ATTTCCTTTG ACAGTGAAAT AATAATGATG AATCATGTGT ACAAAGAAAG GTTTCCAAAG 
GCCACGGCGC AGATGGAAGA ACGGCTGGCT GAGTTTATTG CCTCTAATGC TCCAGAGAAC 
GTGCTGCAAT TAGCAGATGG GGTCCTAAGT TTCATTCATC ATCAAGTTAT TGAACTGGCC 
AGAGATTGCC TAGATAAATC ACGTGAAGGT CTTATTACTT CTCGGTACTT TTATGAGCTG 
CAGGAAAACT TGGAGAAACT TCTCCAGGAT GCCCACGAGC GATCCGAGAG CTCCGAGGTT 
GCCTTCGTTA CCCAACTTGT GAAGAAGCTG ATGATTATCA TTGCACGGCC GGCTCGGCTG 
CTGGAGTGCC TGGAATTTGA TCCTGAAGAG TTCTACCATT TGCTAGAAGC TGCAGAAGGC 
CATGCCAAGG AAGGGCAAGG AATTAAATGT GACATTCCTC GTTATATTAT CAGCCAGTTG 
GGACTCACCA GGGATCCCCT GGAGGAAATG GCCCAGCTGA ATAGCTATGA CAGCCCAGAC 
ACTCCTGAGA CAGATGATTC AGTCGAGAGC CGTGGGGCCT CTGTCCAGTC AAAGAAAACT 
CCATCTGAAG AAGAGTTTGA AACCATTAAA CTGATCAGTA ATGGTGCTTA TGGAGCGGTG 
TATTTGGTGC GACACAAGAC CACCCGTCAA CGTTTTGCCA TGAAGAAGAT CAACAAACAG 
AACCTAATTT TGAGAAACCA GATCCAGCAG GCTTTTGTGG AGAGAGATAT CTTGACGTTT 
GCTGAGAACC CCTTCGTGGT CAGCATGTTC TGCTCCTTTG AGACCAAGCG GCACCTGTGC 
ATGGTTATGG AGTATGTGGA AGGAGGTGAT TGTGCCACCC TTCTCAAGAA CATCGGTGCC 
CTGCCTGTTG ATATGGCGAG GATGTATTTT GCTGAGACTG TTCTGGCATT GGAGTACCTA 
CACAATTATG GGATTGTTCA CCGTGACCTA AAACCTGATA ATCTCCTGAT AACTTCCATG 
GGTCACATTA AACTAACAGA CTTTGGACTG TCTAAAATTG GGTTAATGAG TCTTACAACT 
AACCTTTATG AGGGACACAT TGAGAAGGAT ACCAGGGAGT TCCTGGACAA GCAGGTCTGT 
GGGACTCCAG AATACATCGC GCCAGAAGTG ATCCTGCGCC AGGGCTATGG GAAGCCCGTG 
GACTGGTGGG CCATGGGAGT TATCTTGTAC GAGTTTCTGG TCGGCTGCGT CCCTTTCTTT 
GGGGACACAC CTGAAGAGCT GTTTGGACAA GTGATCAGTG ATGAAATTGC CTGGCCAGAA 
GGTGATGATG CACTGCCGCC AGATGCCCAG GACCTCATTT CTAAGCTTCT CCGCCAAAAT 
CCTCTGGAAA GAATGGGAAC AGGTAGTGCC TTTGAAGTGA AACAGCATCG GTTCTTTAAA 
GATTTGGACT GGAATGGATT ACTTCGGCAG AAAGCTGAAT TCATCCCACA GTTGGAATCT 
GAGGATGACA CAAGCTATTT CGACACCCGT TCAGAACGGT ACCAACACCT GGATTCAGAA 
GAAGAGGAGG ACACTAATGA TGATGATCAT GTGGAAATTC GGCAATTTTC CTCATGCTCA 
CCAAGGTTCA GCAAGGTATA CAGCAGCATG GAACGTCTTT CCATCCACGA AGAGAGGAAG 
ACTCCACCAC CAACTAAACG GAGTCTGAGT GAAGAAAAAG ATGACCGGCT GGACAGCCTT 
GGTGGCTTGA AGAGTCGAGA CCGCAGCTGG GTGATTGGGT CTCCTGAAAT ACTGCGGAAG 
CGCTTGTCTA TGTCTGAATC CTCGCACACG GAGAGTGACT CCAGCCCACC CTTGACCGTC 
CGCCGGCGCT GCTCAGGGCT CCTCGATATG CCCCGTTTTG CCATCTCCAC TGAGGACGAG 
GGAACTGTTC TCAAAAGGCC ACAGTCCGAG GGGATGCTCT TATCCACCGT GCAGTCCCGG 
GAGGGGCTGC CAGTGCCCAT TCCAGAGCAG CCTGTGGAGC AAGAGCTGCA GCTGGAAGGT 
GATGCTGGAC CCACCACCCC CTCCACAGCA GTCAGCAGCG CCTCAACGCT GACAGCTGGG 
AGCACTGCAG ATTTCTCTGA TCCACGCGCT CGCAGTAATA GCAATGAAGG CCCAGACTTT 
ACCACTCCAA AAGCCATCAG CGATTTGGCA GTGCGGCGGG CACGACACAG GTTGCTCTCT 
GGGGAATCAG GGGAAAAACG TACCTCGAGA CCTGTCAATA AAGTGATTAA ATCGGCATCC 
GCTACTGCCT TGTCTCTCCT GATTCCCTCA GATCACCACA CGTGTTCTCC ATTGGCCAGT 
CCAATGTCAC CTCATTCTCT ATCCTCCAAC CCATCTTCGA GGGACTCCTC ACCCAGCCGC 
GACTTTTCTC CTGCTATCGC AAACCTGAAA CCACCAATCA TCATCCATCG GGCTGGAAAG 
AAATACGGTT TCACTCTCCG TGCCATTCGC GTCTATATGG GTGACAGTGA TATCTACACT 
GTGCATCACA TGGTCTGGCA CGTGGAGGAA GGTGGTCCAG CAAACGAAGC AGGTCTGCGT 
GAAGGGGATC TGATAACCCA TGTCAACGGG GAGCCGGTCC ATGGACTGGT GCACACAGAA 
GTGGTGGAAC TGATTCTGAA GAGTGGAAAT AAAGTGTCCA TCTCCACCAC GCCGTTTGAG 
AACACCTCCA TCAAAGTTGG TCCAGCTCGA AAAGCCAGCT ACAAATCCAA GATGGCTCGT 
AGGAACAAGA AGAGCAAAAC TAAGGATGGT CAGGAAAGTA AGAAGAAGAG CTCTTTGTTG 
AGGAAGATCA CTAAACAGGC ATCACTACTT CACACCAGCC GGAGTTTATC TTCACTAAAC 
CGCTCTCTGT CTTCTGGAGA AAGCGTGCCT GGCTCTCCAA CTCACAACCT GTCTCCTCGG 
TCACCAACCC AGAGCTACAG ATCCACTCCC GAGTCTGTAC ACTCAGTTGG TGGCAATTCT 
TCCCAGAGCA GCTCTCCCAG TTCCAGTGTT CCCAATTCTC CAGCCAGCTC TGGACACATC 
CGACCCAGCT CTCTGCACGG ATTAGCACCG AAGCTTCAAA GGCAGTACAG GTCTCCGAGG 
CGCAAATCTG CGGGGAACAT CCCTCTGTCA CCCCTGGCTC ACACTCCCTC ACCCACCCCA 
CAGTCCACCT CACCGCAGCG CTCCCCATCT CCTTTACCAG GACACTCGGT TGGCAGCTCC 
AGTATCATCC AGTCTTTTCC AGTAAAACTA CACTCCTCTC CACCGCTGGT GAGACAAATT 
TCTCGCCCCA AAAGCGCTGA GCCCCCTCGG TCTCCTTTAC TAAAGAGAGT GCAGTCAGCT 
GAGAAGCTGG CTGCCTCGCT GTCTTCTTCT GAGAAGAAGC TGGGCTCCTC GCGTAAGCAC 
AGCCTGGATA TCTCTCACTC AGAATTTAAG AAGGAGATGC TCCAGAGGGA TCCCAGTCTC 
CAGAGTTTAC AAGAATCCGC GAATGAAACA ACGGGTGGCA AACTTGGGCT GGCAGAAAAA 
GGGATGTTGC AGAAGCCGGG TTCACGGAAG TTGGGTGCTA TTCGACAGGA CAGGGTGGAG 
AGGAGGGAGT CTCTGCAAAA GCAAGAGGCC ATCAGGGAAG TAGATTCTTC CGAAGATGAA 
ACGGATGATG GTTCAGAAGA TAGTCAGGAT GGGAGAAGAC TAGACAAGCC CCCTGACAGC 
GGAGACCAAG GCTCAGATTC TTTTAATGAG GATAATAAGT TTTCATCTAA ATTGGAAAGC 
AAGGATAGTA TCGAAGATGA TGCCTTTCTA CCTGAAGATC CAAAAGGTAC GGAAGATTTG 
CAGAAGGGAA ACAGTCAACA AGCCAAGACT ATTTCAGAGC CGCTGCAAAT CAGTGTGCAC 
CCTTCCCCAT CAGAGGTCCC CCCAAGAACT TCTCCAGAAA AATTAGGTAA TTCAGAAAAG 
CCATTCCTGA GCTCAGAAAC AACTGCAAAG GAACATAAAT TGCCAGAAAA CAAAACCTTT 
GAGTGTCTTG GTCCAAAAGA CAAGTCTTCT GAAGCAATCA AAGACCTAGG GAGAACTGCA 
AGTACCCAAA AACCAGTAGA CCGCACAGAG CATGTACAGT GCCCGTCTAT CAAACTCGTG 
GAACTTGAAG AAGGTGACTC TTTGGGGGCC TCGTGTGGTA AGCTTGACCT GAAAGACCCT 
CTGCTAACAG ACAGCAGTCA GAAAAAACAG GATTCCAAAC CTCTGCTGGC ACTTTCTTCG 
TGGTGCACGG CGAGCGTGAG CCCTCCCCTC TCGGTGAGCC CTGCGGATCG CAGTGAGAAG 
GATCACAAGG AGCCGCTTCA GCCTGCAGAA CAGCACAGCA CCTCCTTTCT GCCTCCTGGA 
AGCGTGAGTG AAACCTCCCA AAAAAGTCCT AGTACTGAAA AGCAGCCCAG CTCGTCCCAG 
GCGGAGAGCG CTTCGGCCGC CAGCAAAGCG ACCAGCCCGG CTGCTCCTGT CCCCTCCACG 
CTCCTCGTCC CCGCTGCCAT CGAAAGAGCT CTCTCCGTGT CCCAGTTAGT GCCACTCGCT 
CAGAGCGTGT TAGGCCCTTT GAAGCTCAGT ATGTCTGGTT CCGGAGAGGT GGAAAAGAGA 
AAGGATTTCC CCCATTTGGG TGCCTCCTGT AAAGAAGAGA GAGATTCCAA ACAAAAAAAG 
CAGGAAACTT CTCAAGGAGG AGGAGCGTGT CAAGAGAAAG CCAAACTATC TAGCACAGGC 
GGTCTGGCCA CCAGGAGCAG GTCCTGCACA GAGTCGTTAA ACTCGGCCAC GTGTCCCGTG 
GTGGCAAAAA AGGAGGCAGC TTTTTGCAGC CCTAAAGCAT CTGAGGTGTT GGGAGGTAGC 
TGCAAAACCA CACCATCGAA AGAGCTGTCT CACGCTCTTG GACAAGGAGC TCTGAGAGCC 
TCTCCTCTGC CAGATGGCAG CACGAGGCCC TCTAAAGAAG GGACTCTGGC GCAGGCAAAA 
AGCAAAGAGG TTGGAAATCA GTTAAAAATA CAAGACTTTC CCCTGTCACA TGATGGTGCT 
GTAAAGAAAA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_014939193.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794679.1 Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

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CloneID	OCa62993
Clone ID Related Accession (Same CDS sequence)	XM_014939193.1
Accession Version	XM_014939193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090812.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAACAG AGTCCACTCC ATTACGCTGC AGGAAGCTCA GCAATCCTGA CATCTTTTCA 
TCTGCTGGGA AAACCAAGCT CCATCGTCAG CTAAGTCAAG ATGACTGCAA GCTACGAAGA 
GGTAGTTTGG CAAGTTCTTT GTCAGGAAAA CAGCTACTTC CCTTATCAAA TAGTGTCCAC 
AGTGGAGTGG GACAAACAAT ATGGCAGCCA CCTGGAGATA CCTCAAACCT GGTCCGCATG 
AGAAATCAGT CCCTTGGACA ATCTGCTCCT TCACTTACTG CTGGTTTGAA GGAGCTCAGT 
CTCCCCAGAA GAGGAAGCTT CTGTCGGACA AGCAACAGAA AGAGTTTGAT CGTAACATCC 
AGCACATCTC CAACTCTCCC GCGACCACAT TCCCCTCTTC ATGGACACGC AGGTAGCAGC 
CCTTTGGACA GTCCCAGGAA CTTCTCTCCA AATGCACCTG CTCATTTTTC CTTTGTTCCT 
GCCCGAAGCC ACGGACACAG AGCAGACAGA ACGGATGGGC GCCGCTGGTC CTTGGCCTCC 
CTTCCTTCAT CTGGATATGG AACAAACACT CCCAGTTCAA CAGTTTCATC ATCATGCTCA 
TCTCAGGAGA AGCTGCACCA GCTGCCCTTT CAGCCCACGG CAGATGAACT TCATTTCCTG 
ACAAAGCATT TCAGTACTGA GAGCATCACT GATGAGGAAG GGCGCCATTC TCCAGCCTTG 
AGGCCTCGAT CTCGCAGTCT CAGTGCAGGC TGCATGGTAA TTGCATATGG ATCTCCTAAA 
TGTAGTTACA CAGTAATCTA CAGGAGCAGC CCGGGTCGCT CCCCAATTTC CTTTGACAGT 
GAAATAATAA TGATGAATCA TGTGTACAAA GAAAGGTTTC CAAAGGCCAC GGCGCAGATG 
GAAGAACGGC TGGCTGAGTT TATTGCCTCT AATGCTCCAG AGAACGTGCT GCAATTAGCA 
GATGGGGTCC TAAGTTTCAT TCATCATCAA GTTATTGAAC TGGCCAGAGA TTGCCTAGAT 
AAATCACGTG AAGGTCTTAT TACTTCTCGG TACTTTTATG AGCTGCAGGA AAACTTGGAG 
AAACTTCTCC AGGATGCCCA CGAGCGATCC GAGAGCTCCG AGGTTGCCTT CGTTACCCAA 
CTTGTGAAGA AGCTGATGAT TATCATTGCA CGGCCGGCTC GGCTGCTGGA GTGCCTGGAA 
TTTGATCCTG AAGAGTTCTA CCATTTGCTA GAAGCTGCAG AAGGCCATGC CAAGGAAGGG 
CAAGGAATTA AATGTGACAT TCCTCGTTAT ATTATCAGCC AGTTGGGACT CACCAGGGAT 
CCCCTGGAGG AAATGGCCCA GCTGAATAGC TATGACAGCC CAGACACTCC TGAGACAGAT 
GATTCAGTCG AGAGCCGTGG GGCCTCTGTC CAGTCAAAGA AAACTCCATC TGAAGAAGAG 
TTTGAAACCA TTAAACTGAT CAGTAATGGT GCTTATGGAG CGGTGTATTT GGTGCGACAC 
AAGACCACCC GTCAACGTTT TGCCATGAAG AAGATCAACA AACAGAACCT AATTTTGAGA 
AACCAGATCC AGCAGGCTTT TGTGGAGAGA GATATCTTGA CGTTTGCTGA GAACCCCTTC 
GTGGTCAGCA TGTTCTGCTC CTTTGAGACC AAGCGGCACC TGTGCATGGT TATGGAGTAT 
GTGGAAGGAG GTGATTGTGC CACCCTTCTC AAGAACATCG GTGCCCTGCC TGTTGATATG 
GCGAGGATGT ATTTTGCTGA GACTGTTCTG GCATTGGAGT ACCTACACAA TTATGGGATT 
GTTCACCGTG ACCTAAAACC TGATAATCTC CTGATAACTT CCATGGGTCA CATTAAACTA 
ACAGACTTTG GACTGTCTAA AATTGGGTTA ATGAGTCTTA CAACTAACCT TTATGAGGGA 
CACATTGAGA AGGATACCAG GGAGTTCCTG GACAAGCAGG TCTGTGGGAC TCCAGAATAC 
ATCGCGCCAG AAGTGATCCT GCGCCAGGGC TATGGGAAGC CCGTGGACTG GTGGGCCATG 
GGAGTTATCT TGTACGAGTT TCTGGTCGGC TGCGTCCCTT TCTTTGGGGA CACACCTGAA 
GAGCTGTTTG GACAAGTGAT CAGTGATGAA ATTGCCTGGC CAGAAGGTGA TGATGCACTG 
CCGCCAGATG CCCAGGACCT CATTTCTAAG CTTCTCCGCC AAAATCCTCT GGAAAGAATG 
GGAACAGGTA GTGCCTTTGA AGTGAAACAG CATCGGTTCT TTAAAGATTT GGACTGGAAT 
GGATTACTTC GGCAGAAAGC TGAATTCATC CCACAGTTGG AATCTGAGGA TGACACAAGC 
TATTTCGACA CCCGTTCAGA ACGGTACCAA CACCTGGATT CAGAAGAAGA GGAGGACACT 
AATGATGATG ATCATGTGGA AATTCGGCAA TTTTCCTCAT GCTCACCAAG GTTCAGCAAG 
GTATACAGCA GCATGGAACG TCTTTCCATC CACGAAGAGA GGAAGACTCC ACCACCAACT 
AAACGGAGTC TGAGTGAAGA AAAAGATGAC CGGCTGGACA GCCTTGGTGG CTTGAAGAGT 
CGAGACCGCA GCTGGGTGAT TGGGTCTCCT GAAATACTGC GGAAGCGCTT GTCTATGTCT 
GAATCCTCGC ACACGGAGAG TGACTCCAGC CCACCCTTGA CCGTCCGCCG GCGCTGCTCA 
GGGCTCCTCG ATATGCCCCG TTTTGCCATC TCCACTGAGG ACGAGGGAAC TGTTCTCAAA 
AGGCCACAGT CCGAGGGGAT GCTCTTATCC ACCGTGCAGT CCCGGGAGGG GCTGCCAGTG 
CCCATTCCAG AGCAGCCTGT GGAGCAAGAG CTGCAGCTGG AAGGTGATGC TGGACCCACC 
ACCCCCTCCA CAGCAGTCAG CAGCGCCTCA ACGCTGACAG CTGGGAGCAC TGCAGATTTC 
TCTGATCCAC GCGCTCGCAG TAATAGCAAT GAAGGCCCAG ACTTTACCAC TCCAAAAGCC 
ATCAGCGATT TGGCAGTGCG GCGGGCACGA CACAGGTTGC TCTCTGGGGA ATCAGGGGAA 
AAACGTACCT CGAGACCTGT CAATAAAGTG ATTAAATCGG CATCCGCTAC TGCCTTGTCT 
CTCCTGATTC CCTCAGATCA CCACACGTGT TCTCCATTGG CCAGTCCAAT GTCACCTCAT 
TCTCTATCCT CCAACCCATC TTCGAGGGAC TCCTCACCCA GCCGCGACTT TTCTCCTGCT 
ATCGCAAACC TGAAACCACC AATCATCATC CATCGGGCTG GAAAGAAATA CGGTTTCACT 
CTCCGTGCCA TTCGCGTCTA TATGGGTGAC AGTGATATCT ACACTGTGCA TCACATGGTC 
TGGCACGTGG AGGAAGGTGG TCCAGCAAAC GAAGCAGGTC TGCGTGAAGG GGATCTGATA 
ACCCATGTCA ACGGGGAGCC GGTCCATGGA CTGGTGCACA CAGAAGTGGT GGAACTGATT 
CTGAAGAGTG GAAATAAAGT GTCCATCTCC ACCACGCCGT TTGAGAACAC CTCCATCAAA 
GTTGGTCCAG CTCGAAAAGC CAGCTACAAA TCCAAGATGG CTCGTAGGAA CAAGAAGAGC 
AAAACTAAGG ATGGTCAGGA AAGTAAGAAG AAGAGCTCTT TGTTGAGGAA GATCACTAAA 
CAGGCATCAC TACTTCACAC CAGCCGGAGT TTATCTTCAC TAAACCGCTC TCTGTCTTCT 
GGAGAAAGCG TGCCTGGCTC TCCAACTCAC AACCTGTCTC CTCGGTCACC AACCCAGAGC 
TACAGATCCA CTCCCGAGTC TGTACACTCA GTTGGTGGCA ATTCTTCCCA GAGCAGCTCT 
CCCAGTTCCA GTGTTCCCAA TTCTCCAGCC AGCTCTGGAC ACATCCGACC CAGCTCTCTG 
CACGGATTAG CACCGAAGCT TCAAAGGCAG TACAGGTCTC CGAGGCGCAA ATCTGCGGGG 
AACATCCCTC TGTCACCCCT GGCTCACACT CCCTCACCCA CCCCACAGTC CACCTCACCG 
CAGCGCTCCC CATCTCCTTT ACCAGGACAC TCGGTTGGCA GCTCCAGTAT CATCCAGTCT 
TTTCCAGTAA AACTACACTC CTCTCCACCG CTGGTGAGAC AAATTTCTCG CCCCAAAAGC 
GCTGAGCCCC CTCGGTCTCC TTTACTAAAG AGAGTGCAGT CAGCTGAGAA GCTGGCTGCC 
TCGCTGTCTT CTTCTGAGAA GAAGCTGGGC TCCTCGCGTA AGCACAGCCT GGATATCTCT 
CACTCAGAAT TTAAGAAGGA GATGCTCCAG AGGGATCCCA GTCTCCAGAG TTTACAAGAA 
TCCGCGAATG AAACAACGGG TGGCAAACTT GGGCTGGCAG AAAAAGGGAT GTTGCAGAAG 
CCGGGTTCAC GGAAGTTGGG TGCTATTCGA CAGGACAGGG TGGAGAGGAG GGAGTCTCTG 
CAAAAGCAAG AGGCCATCAG GGAAGTAGAT TCTTCCGAAG ATGAAACGGA TGATGGTTCA 
GAAGATAGTC AGGATGGGAG AAGACTAGAC AAGCCCCCTG ACAGCGGAGA CCAAGGCTCA 
GATTCTTTTA ATGAGGATAA TAAGTTTTCA TCTAAATTGG AAAGCAAGGA TAGTATCGAA 
GATGATGCCT TTCTACCTGA AGATCCAAAA GGTACGGAAG ATTTGCAGAA GGGAAACAGT 
CAACAAGCCA AGACTATTTC AGAGCCGCTG CAAATCAGTG TGCACCCTTC CCCATCAGAG 
GTCCCCCCAA GAACTTCTCC AGAAAAATTA GGTAATTCAG AAAAGCCATT CCTGAGCTCA 
GAAACAACTG CAAAGGAACA TAAATTGCCA GAAAACAAAA CCTTTGAGTG TCTTGGTCCA 
AAAGACAAGT CTTCTGAAGC AATCAAAGAC CTAGGGAGAA CTGCAAGTAC CCAAAAACCA 
GTAGACCGCA CAGAGCATGT ACAGTGCCCG TCTATCAAAC TCGTGGAACT TGAAGAAGGT 
GACTCTTTGG GGGCCTCGTG TGGTAAGCTT GACCTGAAAG ACCCTCTGCT AACAGACAGC 
AGTCAGAAAA AACAGGATTC CAAACCTCTG CTGGCACTTT CTTCGTGGTG CACGGCGAGC 
GTGAGCCCTC CCCTCTCGGT GAGCCCTGCG GATCGCAGTG AGAAGGATCA CAAGGAGCCG 
CTTCAGCCTG CAGAACAGCA CAGCACCTCC TTTCTGCCTC CTGGAAGCGT GAGTGAAACC 
TCCCAAAAAA GTCCTAGTAC TGAAAAGCAG CCCAGCTCGT CCCAGGCGGA GAGCGCTTCG 
GCCGCCAGCA AAGCGACCAG CCCGGCTGCT CCTGTCCCCT CCACGCTCCT CGTCCCCGCT 
GCCATCGAAA GAGCTCTCTC CGTGTCCCAG TTAGTGCCAC TCGCTCAGAG CGTGTTAGGC 
CCTTTGAAGC TCAGTATGTC TGGTTCCGGA GAGGTGGAAA AGAGAAAGGA TTTCCCCCAT 
TTGGGTGCCT CCTGTAAAGA AGAGAGAGAT TCCAAACAAA AAAAGCAGGA AACTTCTCAA 
GGAGGAGGAG CGTGTCAAGA GAAAGCCAAA CTATCTAGCA CAGGCGGTCT GGCCACCAGG 
AGCAGGTCCT GCACAGAGTC GTTAAACTCG GCCACGTGTC CCGTGGTGGC AAAAAAGGAG 
GCAGCTTTTT GCAGCCCTAA AGCATCTGAG GTGTTGGGAG GTAGCTGCAA AACCACACCA 
TCGAAAGAGC TGTCTCACGC TCTTGGACAA GGAGCTCTGA GAGCCTCTCC TCTGCCAGAT 
GGCAGCACGA GGCCCTCTAA AGAAGGGACT CTGGCGCAGG CAAAAAGCAA AGAGGTTGGA 
AATCAGTTAA AAATACAAGA CTTTCCCCTG TCACATGATG GTGCTGTAAA GAAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794679.1
CDS	4..5883
Translation	MITESTPLRCRKLSNPDIFSSAGKTKLHRQLSQDDCKLRRGSLASSLSGKQLLPLSNSVHSGVGQTIWQPPGDTSNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHAGSSPLDSPRNFSPNAPAHFSFVPARSHGHRADRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTESITDEEGRHSPALRPRSRSLSAGCMVIAYGSPKCSYTVIYRSSPGRSPISFDSEIIMMNHVYKERFPKATAQMEERLAEFIASNAPENVLQLADGVLSFIHHQVIELARDCLDKSREGLITSRYFYELQENLEKLLQDAHERSESSEVAFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIISQLGLTRDPLEEMAQLNSYDSPDTPETDDSVESRGASVQSKKTPSEEEFETIKLISNGAYGAVYLVRHKTTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVISDEIAWPEGDDALPPDAQDLISKLLRQNPLERMGTGSAFEVKQHRFFKDLDWNGLLRQKAEFIPQLESEDDTSYFDTRSERYQHLDSEEEEDTNDDDHVEIRQFSSCSPRFSKVYSSMERLSIHEERKTPPPTKRSLSEEKDDRLDSLGGLKSRDRSWVIGSPEILRKRLSMSESSHTESDSSPPLTVRRRCSGLLDMPRFAISTEDEGTVLKRPQSEGMLLSTVQSREGLPVPIPEQPVEQELQLEGDAGPTTPSTAVSSASTLTAGSTADFSDPRARSNSNEGPDFTTPKAISDLAVRRARHRLLSGESGEKRTSRPVNKVIKSASATALSLLIPSDHHTCSPLASPMSPHSLSSNPSSRDSSPSRDFSPAIANLKPPIIIHRAGKKYGFTLRAIRVYMGDSDIYTVHHMVWHVEEGGPANEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPFENTSIKVGPARKASYKSKMARRNKKSKTKDGQESKKKSSLLRKITKQASLLHTSRSLSSLNRSLSSGESVPGSPTHNLSPRSPTQSYRSTPESVHSVGGNSSQSSSPSSSVPNSPASSGHIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLAHTPSPTPQSTSPQRSPSPLPGHSVGSSSIIQSFPVKLHSSPPLVRQISRPKSAEPPRSPLLKRVQSAEKLAASLSSSEKKLGSSRKHSLDISHSEFKKEMLQRDPSLQSLQESANETTGGKLGLAEKGMLQKPGSRKLGAIRQDRVERRESLQKQEAIREVDSSEDETDDGSEDSQDGRRLDKPPDSGDQGSDSFNEDNKFSSKLESKDSIEDDAFLPEDPKGTEDLQKGNSQQAKTISEPLQISVHPSPSEVPPRTSPEKLGNSEKPFLSSETTAKEHKLPENKTFECLGPKDKSSEAIKDLGRTASTQKPVDRTEHVQCPSIKLVELEEGDSLGASCGKLDLKDPLLTDSSQKKQDSKPLLALSSWCTASVSPPLSVSPADRSEKDHKEPLQPAEQHSTSFLPPGSVSETSQKSPSTEKQPSSSQAESASAASKATSPAAPVPSTLLVPAAIERALSVSQLVPLAQSVLGPLKLSMSGSGEVEKRKDFPHLGASCKEERDSKQKKQETSQGGGACQEKAKLSSTGGLATRSRSCTESLNSATCPVVAKKEAAFCSPKASEVLGGSCKTTPSKELSHALGQGALRASPLPDGSTRPSKEGTLAQAKSKEVGNQLKIQDFPLSHDGAVKKT

Target ORF information:

RefSeq Version	XM_014939193.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939193.1

ORF Insert Sequence:

ATGATAACAG AGTCCACTCC ATTACGCTGC AGGAAGCTCA GCAATCCTGA CATCTTTTCA 
TCTGCTGGGA AAACCAAGCT CCATCGTCAG CTAAGTCAAG ATGACTGCAA GCTACGAAGA 
GGTAGTTTGG CAAGTTCTTT GTCAGGAAAA CAGCTACTTC CCTTATCAAA TAGTGTCCAC 
AGTGGAGTGG GACAAACAAT ATGGCAGCCA CCTGGAGATA CCTCAAACCT GGTCCGCATG 
AGAAATCAGT CCCTTGGACA ATCTGCTCCT TCACTTACTG CTGGTTTGAA GGAGCTCAGT 
CTCCCCAGAA GAGGAAGCTT CTGTCGGACA AGCAACAGAA AGAGTTTGAT CGTAACATCC 
AGCACATCTC CAACTCTCCC GCGACCACAT TCCCCTCTTC ATGGACACGC AGGTAGCAGC 
CCTTTGGACA GTCCCAGGAA CTTCTCTCCA AATGCACCTG CTCATTTTTC CTTTGTTCCT 
GCCCGAAGCC ACGGACACAG AGCAGACAGA ACGGATGGGC GCCGCTGGTC CTTGGCCTCC 
CTTCCTTCAT CTGGATATGG AACAAACACT CCCAGTTCAA CAGTTTCATC ATCATGCTCA 
TCTCAGGAGA AGCTGCACCA GCTGCCCTTT CAGCCCACGG CAGATGAACT TCATTTCCTG 
ACAAAGCATT TCAGTACTGA GAGCATCACT GATGAGGAAG GGCGCCATTC TCCAGCCTTG 
AGGCCTCGAT CTCGCAGTCT CAGTGCAGGC TGCATGGTAA TTGCATATGG ATCTCCTAAA 
TGTAGTTACA CAGTAATCTA CAGGAGCAGC CCGGGTCGCT CCCCAATTTC CTTTGACAGT 
GAAATAATAA TGATGAATCA TGTGTACAAA GAAAGGTTTC CAAAGGCCAC GGCGCAGATG 
GAAGAACGGC TGGCTGAGTT TATTGCCTCT AATGCTCCAG AGAACGTGCT GCAATTAGCA 
GATGGGGTCC TAAGTTTCAT TCATCATCAA GTTATTGAAC TGGCCAGAGA TTGCCTAGAT 
AAATCACGTG AAGGTCTTAT TACTTCTCGG TACTTTTATG AGCTGCAGGA AAACTTGGAG 
AAACTTCTCC AGGATGCCCA CGAGCGATCC GAGAGCTCCG AGGTTGCCTT CGTTACCCAA 
CTTGTGAAGA AGCTGATGAT TATCATTGCA CGGCCGGCTC GGCTGCTGGA GTGCCTGGAA 
TTTGATCCTG AAGAGTTCTA CCATTTGCTA GAAGCTGCAG AAGGCCATGC CAAGGAAGGG 
CAAGGAATTA AATGTGACAT TCCTCGTTAT ATTATCAGCC AGTTGGGACT CACCAGGGAT 
CCCCTGGAGG AAATGGCCCA GCTGAATAGC TATGACAGCC CAGACACTCC TGAGACAGAT 
GATTCAGTCG AGAGCCGTGG GGCCTCTGTC CAGTCAAAGA AAACTCCATC TGAAGAAGAG 
TTTGAAACCA TTAAACTGAT CAGTAATGGT GCTTATGGAG CGGTGTATTT GGTGCGACAC 
AAGACCACCC GTCAACGTTT TGCCATGAAG AAGATCAACA AACAGAACCT AATTTTGAGA 
AACCAGATCC AGCAGGCTTT TGTGGAGAGA GATATCTTGA CGTTTGCTGA GAACCCCTTC 
GTGGTCAGCA TGTTCTGCTC CTTTGAGACC AAGCGGCACC TGTGCATGGT TATGGAGTAT 
GTGGAAGGAG GTGATTGTGC CACCCTTCTC AAGAACATCG GTGCCCTGCC TGTTGATATG 
GCGAGGATGT ATTTTGCTGA GACTGTTCTG GCATTGGAGT ACCTACACAA TTATGGGATT 
GTTCACCGTG ACCTAAAACC TGATAATCTC CTGATAACTT CCATGGGTCA CATTAAACTA 
ACAGACTTTG GACTGTCTAA AATTGGGTTA ATGAGTCTTA CAACTAACCT TTATGAGGGA 
CACATTGAGA AGGATACCAG GGAGTTCCTG GACAAGCAGG TCTGTGGGAC TCCAGAATAC 
ATCGCGCCAG AAGTGATCCT GCGCCAGGGC TATGGGAAGC CCGTGGACTG GTGGGCCATG 
GGAGTTATCT TGTACGAGTT TCTGGTCGGC TGCGTCCCTT TCTTTGGGGA CACACCTGAA 
GAGCTGTTTG GACAAGTGAT CAGTGATGAA ATTGCCTGGC CAGAAGGTGA TGATGCACTG 
CCGCCAGATG CCCAGGACCT CATTTCTAAG CTTCTCCGCC AAAATCCTCT GGAAAGAATG 
GGAACAGGTA GTGCCTTTGA AGTGAAACAG CATCGGTTCT TTAAAGATTT GGACTGGAAT 
GGATTACTTC GGCAGAAAGC TGAATTCATC CCACAGTTGG AATCTGAGGA TGACACAAGC 
TATTTCGACA CCCGTTCAGA ACGGTACCAA CACCTGGATT CAGAAGAAGA GGAGGACACT 
AATGATGATG ATCATGTGGA AATTCGGCAA TTTTCCTCAT GCTCACCAAG GTTCAGCAAG 
GTATACAGCA GCATGGAACG TCTTTCCATC CACGAAGAGA GGAAGACTCC ACCACCAACT 
AAACGGAGTC TGAGTGAAGA AAAAGATGAC CGGCTGGACA GCCTTGGTGG CTTGAAGAGT 
CGAGACCGCA GCTGGGTGAT TGGGTCTCCT GAAATACTGC GGAAGCGCTT GTCTATGTCT 
GAATCCTCGC ACACGGAGAG TGACTCCAGC CCACCCTTGA CCGTCCGCCG GCGCTGCTCA 
GGGCTCCTCG ATATGCCCCG TTTTGCCATC TCCACTGAGG ACGAGGGAAC TGTTCTCAAA 
AGGCCACAGT CCGAGGGGAT GCTCTTATCC ACCGTGCAGT CCCGGGAGGG GCTGCCAGTG 
CCCATTCCAG AGCAGCCTGT GGAGCAAGAG CTGCAGCTGG AAGGTGATGC TGGACCCACC 
ACCCCCTCCA CAGCAGTCAG CAGCGCCTCA ACGCTGACAG CTGGGAGCAC TGCAGATTTC 
TCTGATCCAC GCGCTCGCAG TAATAGCAAT GAAGGCCCAG ACTTTACCAC TCCAAAAGCC 
ATCAGCGATT TGGCAGTGCG GCGGGCACGA CACAGGTTGC TCTCTGGGGA ATCAGGGGAA 
AAACGTACCT CGAGACCTGT CAATAAAGTG ATTAAATCGG CATCCGCTAC TGCCTTGTCT 
CTCCTGATTC CCTCAGATCA CCACACGTGT TCTCCATTGG CCAGTCCAAT GTCACCTCAT 
TCTCTATCCT CCAACCCATC TTCGAGGGAC TCCTCACCCA GCCGCGACTT TTCTCCTGCT 
ATCGCAAACC TGAAACCACC AATCATCATC CATCGGGCTG GAAAGAAATA CGGTTTCACT 
CTCCGTGCCA TTCGCGTCTA TATGGGTGAC AGTGATATCT ACACTGTGCA TCACATGGTC 
TGGCACGTGG AGGAAGGTGG TCCAGCAAAC GAAGCAGGTC TGCGTGAAGG GGATCTGATA 
ACCCATGTCA ACGGGGAGCC GGTCCATGGA CTGGTGCACA CAGAAGTGGT GGAACTGATT 
CTGAAGAGTG GAAATAAAGT GTCCATCTCC ACCACGCCGT TTGAGAACAC CTCCATCAAA 
GTTGGTCCAG CTCGAAAAGC CAGCTACAAA TCCAAGATGG CTCGTAGGAA CAAGAAGAGC 
AAAACTAAGG ATGGTCAGGA AAGTAAGAAG AAGAGCTCTT TGTTGAGGAA GATCACTAAA 
CAGGCATCAC TACTTCACAC CAGCCGGAGT TTATCTTCAC TAAACCGCTC TCTGTCTTCT 
GGAGAAAGCG TGCCTGGCTC TCCAACTCAC AACCTGTCTC CTCGGTCACC AACCCAGAGC 
TACAGATCCA CTCCCGAGTC TGTACACTCA GTTGGTGGCA ATTCTTCCCA GAGCAGCTCT 
CCCAGTTCCA GTGTTCCCAA TTCTCCAGCC AGCTCTGGAC ACATCCGACC CAGCTCTCTG 
CACGGATTAG CACCGAAGCT TCAAAGGCAG TACAGGTCTC CGAGGCGCAA ATCTGCGGGG 
AACATCCCTC TGTCACCCCT GGCTCACACT CCCTCACCCA CCCCACAGTC CACCTCACCG 
CAGCGCTCCC CATCTCCTTT ACCAGGACAC TCGGTTGGCA GCTCCAGTAT CATCCAGTCT 
TTTCCAGTAA AACTACACTC CTCTCCACCG CTGGTGAGAC AAATTTCTCG CCCCAAAAGC 
GCTGAGCCCC CTCGGTCTCC TTTACTAAAG AGAGTGCAGT CAGCTGAGAA GCTGGCTGCC 
TCGCTGTCTT CTTCTGAGAA GAAGCTGGGC TCCTCGCGTA AGCACAGCCT GGATATCTCT 
CACTCAGAAT TTAAGAAGGA GATGCTCCAG AGGGATCCCA GTCTCCAGAG TTTACAAGAA 
TCCGCGAATG AAACAACGGG TGGCAAACTT GGGCTGGCAG AAAAAGGGAT GTTGCAGAAG 
CCGGGTTCAC GGAAGTTGGG TGCTATTCGA CAGGACAGGG TGGAGAGGAG GGAGTCTCTG 
CAAAAGCAAG AGGCCATCAG GGAAGTAGAT TCTTCCGAAG ATGAAACGGA TGATGGTTCA 
GAAGATAGTC AGGATGGGAG AAGACTAGAC AAGCCCCCTG ACAGCGGAGA CCAAGGCTCA 
GATTCTTTTA ATGAGGATAA TAAGTTTTCA TCTAAATTGG AAAGCAAGGA TAGTATCGAA 
GATGATGCCT TTCTACCTGA AGATCCAAAA GGTACGGAAG ATTTGCAGAA GGGAAACAGT 
CAACAAGCCA AGACTATTTC AGAGCCGCTG CAAATCAGTG TGCACCCTTC CCCATCAGAG 
GTCCCCCCAA GAACTTCTCC AGAAAAATTA GGTAATTCAG AAAAGCCATT CCTGAGCTCA 
GAAACAACTG CAAAGGAACA TAAATTGCCA GAAAACAAAA CCTTTGAGTG TCTTGGTCCA 
AAAGACAAGT CTTCTGAAGC AATCAAAGAC CTAGGGAGAA CTGCAAGTAC CCAAAAACCA 
GTAGACCGCA CAGAGCATGT ACAGTGCCCG TCTATCAAAC TCGTGGAACT TGAAGAAGGT 
GACTCTTTGG GGGCCTCGTG TGGTAAGCTT GACCTGAAAG ACCCTCTGCT AACAGACAGC 
AGTCAGAAAA AACAGGATTC CAAACCTCTG CTGGCACTTT CTTCGTGGTG CACGGCGAGC 
GTGAGCCCTC CCCTCTCGGT GAGCCCTGCG GATCGCAGTG AGAAGGATCA CAAGGAGCCG 
CTTCAGCCTG CAGAACAGCA CAGCACCTCC TTTCTGCCTC CTGGAAGCGT GAGTGAAACC 
TCCCAAAAAA GTCCTAGTAC TGAAAAGCAG CCCAGCTCGT CCCAGGCGGA GAGCGCTTCG 
GCCGCCAGCA AAGCGACCAG CCCGGCTGCT CCTGTCCCCT CCACGCTCCT CGTCCCCGCT 
GCCATCGAAA GAGCTCTCTC CGTGTCCCAG TTAGTGCCAC TCGCTCAGAG CGTGTTAGGC 
CCTTTGAAGC TCAGTATGTC TGGTTCCGGA GAGGTGGAAA AGAGAAAGGA TTTCCCCCAT 
TTGGGTGCCT CCTGTAAAGA AGAGAGAGAT TCCAAACAAA AAAAGCAGGA AACTTCTCAA 
GGAGGAGGAG CGTGTCAAGA GAAAGCCAAA CTATCTAGCA CAGGCGGTCT GGCCACCAGG 
AGCAGGTCCT GCACAGAGTC GTTAAACTCG GCCACGTGTC CCGTGGTGGC AAAAAAGGAG 
GCAGCTTTTT GCAGCCCTAA AGCATCTGAG GTGTTGGGAG GTAGCTGCAA AACCACACCA 
TCGAAAGAGC TGTCTCACGC TCTTGGACAA GGAGCTCTGA GAGCCTCTCC TCTGCCAGAT 
GGCAGCACGA GGCCCTCTAA AGAAGGGACT CTGGCGCAGG CAAAAAGCAA AGAGGTTGGA 
AATCAGTTAA AAATACAAGA CTTTCCCCTG TCACATGATG GTGCTGTAAA GAAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST2 ( XM_014939194.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794680.1 Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

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CloneID	OCa62994
Clone ID Related Accession (Same CDS sequence)	XM_014939194.1
Accession Version	XM_014939194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microtubule-associated serine/threonine-protein kinase 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090812.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAACAG AGTCCACTCC ATTACGCTGC AGGAAGCTCA GCAATCCTGA CATCTTTTCA 
TCTGCTGGGA AAACCAAGCT CCATCGTCAG CTAAGTCAAG ATGACTGCAA GCTACGAAGA 
GGTAGTTTGG CAAGTTCTTT GTCAGGAAAA CAGCTACTTC CCTTATCAAA TAGTGTCCAC 
AGTGGAGTGG GACAAACAAT ATGGCAGCCA CCTGGAGATA CCTCAAACCT GGTCCGCATG 
AGAAATCAGT CCCTTGGACA ATCTGCTCCT TCACTTACTG CTGGTTTGAA GGAGCTCAGT 
CTCCCCAGAA GAGGAAGCTT CTGTCGGACA AGCAACAGAA AGAGTTTGAT CGTAACATCC 
AGCACATCTC CAACTCTCCC GCGACCACAT TCCCCTCTTC ATGGACACGC AGGTAGCAGC 
CCTTTGGACA GTCCCAGGAA CTTCTCTCCA AATGCACCTG CTCATTTTTC CTTTGTTCCT 
GCCCGAAGAA CGGATGGGCG CCGCTGGTCC TTGGCCTCCC TTCCTTCATC TGGATATGGA 
ACAAACACTC CCAGTTCAAC AGTTTCATCA TCATGCTCAT CTCAGGAGAA GCTGCACCAG 
CTGCCCTTTC AGCCCACGGC AGATGAACTT CATTTCCTGA CAAAGCATTT CAGTACTGAG 
AGCATCACTG ATGAGGAAGG GCGCCATTCT CCAGCCTTGA GGCCTCGATC TCGCAGTCTC 
AGCCCGGGTC GCTCCCCAAT TTCCTTTGAC AGTGAAATAA TAATGATGAA TCATGTGTAC 
AAAGAAAGGT TTCCAAAGGC CACGGCGCAG ATGGAAGAAC GGCTGGCTGA GTTTATTGCC 
TCTAATGCTC CAGAGAACGT GCTGCAATTA GCAGATGGGG TCCTAAGTTT CATTCATCAT 
CAAGTTATTG AACTGGCCAG AGATTGCCTA GATAAATCAC GTGAAGGTCT TATTACTTCT 
CGGTACTTTT ATGAGCTGCA GGAAAACTTG GAGAAACTTC TCCAGGATGC CCACGAGCGA 
TCCGAGAGCT CCGAGGTTGC CTTCGTTACC CAACTTGTGA AGAAGCTGAT GATTATCATT 
GCACGGCCGG CTCGGCTGCT GGAGTGCCTG GAATTTGATC CTGAAGAGTT CTACCATTTG 
CTAGAAGCTG CAGAAGGCCA TGCCAAGGAA GGGCAAGGAA TTAAATGTGA CATTCCTCGT 
TATATTATCA GCCAGTTGGG ACTCACCAGG GATCCCCTGG AGGAAATGGC CCAGCTGAAT 
AGCTATGACA GCCCAGACAC TCCTGAGACA GATGATTCAG TCGAGAGCCG TGGGGCCTCT 
GTCCAGTCAA AGAAAACTCC ATCTGAAGAA GAGTTTGAAA CCATTAAACT GATCAGTAAT 
GGTGCTTATG GAGCGGTGTA TTTGGTGCGA CACAAGACCA CCCGTCAACG TTTTGCCATG 
AAGAAGATCA ACAAACAGAA CCTAATTTTG AGAAACCAGA TCCAGCAGGC TTTTGTGGAG 
AGAGATATCT TGACGTTTGC TGAGAACCCC TTCGTGGTCA GCATGTTCTG CTCCTTTGAG 
ACCAAGCGGC ACCTGTGCAT GGTTATGGAG TATGTGGAAG GAGGTGATTG TGCCACCCTT 
CTCAAGAACA TCGGTGCCCT GCCTGTTGAT ATGGCGAGGA TGTATTTTGC TGAGACTGTT 
CTGGCATTGG AGTACCTACA CAATTATGGG ATTGTTCACC GTGACCTAAA ACCTGATAAT 
CTCCTGATAA CTTCCATGGG TCACATTAAA CTAACAGACT TTGGACTGTC TAAAATTGGG 
TTAATGAGTC TTACAACTAA CCTTTATGAG GGACACATTG AGAAGGATAC CAGGGAGTTC 
CTGGACAAGC AGGTCTGTGG GACTCCAGAA TACATCGCGC CAGAAGTGAT CCTGCGCCAG 
GGCTATGGGA AGCCCGTGGA CTGGTGGGCC ATGGGAGTTA TCTTGTACGA GTTTCTGGTC 
GGCTGCGTCC CTTTCTTTGG GGACACACCT GAAGAGCTGT TTGGACAAGT GATCAGTGAT 
GAAATTGCCT GGCCAGAAGG TGATGATGCA CTGCCGCCAG ATGCCCAGGA CCTCATTTCT 
AAGCTTCTCC GCCAAAATCC TCTGGAAAGA ATGGGAACAG GTAGTGCCTT TGAAGTGAAA 
CAGCATCGGT TCTTTAAAGA TTTGGACTGG AATGGATTAC TTCGGCAGAA AGCTGAATTC 
ATCCCACAGT TGGAATCTGA GGATGACACA AGCTATTTCG ACACCCGTTC AGAACGGTAC 
CAACACCTGG ATTCAGAAGA AGAGGAGGAC ACTAATGATG ATGATCATGT GGAAATTCGG 
CAATTTTCCT CATGCTCACC AAGGTTCAGC AAGGTATACA GCAGCATGGA ACGTCTTTCC 
ATCCACGAAG AGAGGAAGAC TCCACCACCA ACTAAACGGA GTCTGAGTGA AGAAAAAGAT 
GACCGGCTGG ACAGCCTTGG TGGCTTGAAG AGTCGAGACC GCAGCTGGGT GATTGGGTCT 
CCTGAAATAC TGCGGAAGCG CTTGTCTATG TCTGAATCCT CGCACACGGA GAGTGACTCC 
AGCCCACCCT TGACCGTCCG CCGGCGCTGC TCAGGGCTCC TCGATATGCC CCGTTTTGCC 
ATCTCCACTG AGGACGAGGG AACTGTTCTC AAAAGGCCAC AGTCCGAGGG GATGCTCTTA 
TCCACCGTGC AGTCCCGGGA GGGGCTGCCA GTGCCCATTC CAGAGCAGCC TGTGGAGCAA 
GAGCTGCAGC TGGAAGGTGA TGCTGGACCC ACCACCCCCT CCACAGCAGT CAGCAGCGCC 
TCAACGCTGA CAGCTGGGAG CACTGCAGAT TTCTCTGATC CACGCGCTCG CAGTAATAGC 
AATGAAGGCC CAGACTTTAC CACTCCAAAA GCCATCAGCG ATTTGGCAGT GCGGCGGGCA 
CGACACAGGT TGCTCTCTGG GGAATCAGGG GAAAAACGTA CCTCGAGACC TGTCAATAAA 
GTGATTAAAT CGGCATCCGC TACTGCCTTG TCTCTCCTGA TTCCCTCAGA TCACCACACG 
TGTTCTCCAT TGGCCAGTCC AATGTCACCT CATTCTCTAT CCTCCAACCC ATCTTCGAGG 
GACTCCTCAC CCAGCCGCGA CTTTTCTCCT GCTATCGCAA ACCTGAAACC ACCAATCATC 
ATCCATCGGG CTGGAAAGAA ATACGGTTTC ACTCTCCGTG CCATTCGCGT CTATATGGGT 
GACAGTGATA TCTACACTGT GCATCACATG GTCTGGCACG TGGAGGAAGG TGGTCCAGCA 
AACGAAGCAG GTCTGCGTGA AGGGGATCTG ATAACCCATG TCAACGGGGA GCCGGTCCAT 
GGACTGGTGC ACACAGAAGT GGTGGAACTG ATTCTGAAGA GTGGAAATAA AGTGTCCATC 
TCCACCACGC CGTTTGAGAA CACCTCCATC AAAGTTGGTC CAGCTCGAAA AGCCAGCTAC 
AAATCCAAGA TGGCTCGTAG GAACAAGAAG AGCAAAACTA AGGATGGTCA GGAAAGTAAG 
AAGAAGAGCT CTTTGTTGAG GAAGATCACT AAACAGGCAT CACTACTTCA CACCAGCCGG 
AGTTTATCTT CACTAAACCG CTCTCTGTCT TCTGGAGAAA GCGTGCCTGG CTCTCCAACT 
CACAACCTGT CTCCTCGGTC ACCAACCCAG AGCTACAGAT CCACTCCCGA GTCTGTACAC 
TCAGTTGGTG GCAATTCTTC CCAGAGCAGC TCTCCCAGTT CCAGTGTTCC CAATTCTCCA 
GCCAGCTCTG GACACATCCG ACCCAGCTCT CTGCACGGAT TAGCACCGAA GCTTCAAAGG 
CAGTACAGGT CTCCGAGGCG CAAATCTGCG GGGAACATCC CTCTGTCACC CCTGGCTCAC 
ACTCCCTCAC CCACCCCACA GTCCACCTCA CCGCAGCGCT CCCCATCTCC TTTACCAGGA 
CACTCGGTTG GCAGCTCCAG TATCATCCAG TCTTTTCCAG TAAAACTACA CTCCTCTCCA 
CCGCTGGTGA GACAAATTTC TCGCCCCAAA AGCGCTGAGC CCCCTCGGTC TCCTTTACTA 
AAGAGAGTGC AGTCAGCTGA GAAGCTGGCT GCCTCGCTGT CTTCTTCTGA GAAGAAGCTG 
GGCTCCTCGC GTAAGCACAG CCTGGATATC TCTCACTCAG AATTTAAGAA GGAGATGCTC 
CAGAGGGATC CCAGTCTCCA GAGTTTACAA GAATCCGCGA ATGAAACAAC GGGTGGCAAA 
CTTGGGCTGG CAGAAAAAGG GATGTTGCAG AAGCCGGGTT CACGGAAGTT GGGTGCTATT 
CGACAGGACA GGGTGGAGAG GAGGGAGTCT CTGCAAAAGC AAGAGGCCAT CAGGGAAGTA 
GATTCTTCCG AAGATGAAAC GGATGATGGT TCAGAAGATA GTCAGGATGG GAGAAGACTA 
GACAAGCCCC CTGACAGCGG AGACCAAGGC TCAGATTCTT TTAATGAGGA TAATAAGTTT 
TCATCTAAAT TGGAAAGCAA GGATAGTATC GAAGATGATG CCTTTCTACC TGAAGATCCA 
AAAGGTACGG AAGATTTGCA GAAGGGAAAC AGTCAACAAG CCAAGACTAT TTCAGAGCCG 
CTGCAAATCA GTGTGCACCC TTCCCCATCA GAGGTCCCCC CAAGAACTTC TCCAGAAAAA 
TTAGGTAATT CAGAAAAGCC ATTCCTGAGC TCAGAAACAA CTGCAAAGGA ACATAAATTG 
CCAGAAAACA AAACCTTTGA GTGTCTTGGT CCAAAAGACA AGTCTTCTGA AGCAATCAAA 
GACCTAGGGA GAACTGCAAG TACCCAAAAA CCAGTAGACC GCACAGAGCA TGTACAGTGC 
CCGTCTATCA AACTCGTGGA ACTTGAAGAA GGTGACTCTT TGGGGGCCTC GTGTGGTAAG 
CTTGACCTGA AAGACCCTCT GCTAACAGAC AGCAGTCAGA AAAAACAGGA TTCCAAACCT 
CTGCTGGCAC TTTCTTCGTG GTGCACGGCG AGCGTGAGCC CTCCCCTCTC GGTGAGCCCT 
GCGGATCGCA GTGAGAAGGA TCACAAGGAG CCGCTTCAGC CTGCAGAACA GCACAGCACC 
TCCTTTCTGC CTCCTGGAAG CGTGAGTGAA ACCTCCCAAA AAAGTCCTAG TACTGAAAAG 
CAGCCCAGCT CGTCCCAGGC GGAGAGCGCT TCGGCCGCCA GCAAAGCGAC CAGCCCGGCT 
GCTCCTGTCC CCTCCACGCT CCTCGTCCCC GCTGCCATCG AAAGAGCTCT CTCCGTGTCC 
CAGTTAGTGC CACTCGCTCA GAGCGTGTTA GGCCCTTTGA AGCTCAGTAT GTCTGGTTCC 
GGAGAGGTGG AAAAGAGAAA GGATTTCCCC CATTTGGGTG CCTCCTGTAA AGAAGAGAGA 
GATTCCAAAC AAAAAAAGCA GGAAACTTCT CAAGGAGGAG GAGCGTGTCA AGAGAAAGCC 
AAACTATCTA GCACAGGCGG TCTGGCCACC AGGAGCAGGT CCTGCACAGA GTCGTTAAAC 
TCGGCCACGT GTCCCGTGGT GGCAAAAAAG GAGGCAGCTT TTTGCAGCCC TAAAGCATCT 
GAGGTGTTGG GAGGTAGCTG CAAAACCACA CCATCGAAAG AGCTGTCTCA CGCTCTTGGA 
CAAGGAGCTC TGAGAGCCTC TCCTCTGCCA GATGGCAGCA CGAGGCCCTC TAAAGAAGGG 
ACTCTGGCGC AGGCAAAAAG CAAAGAGGTT GGAAATCAGT TAAAAATACA AGACTTTCCC 
CTGTCACATG ATGGTGCTGT AAAGAAAACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794680.1
CDS	4..5796
Translation	MITESTPLRCRKLSNPDIFSSAGKTKLHRQLSQDDCKLRRGSLASSLSGKQLLPLSNSVHSGVGQTIWQPPGDTSNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHAGSSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTESITDEEGRHSPALRPRSRSLSPGRSPISFDSEIIMMNHVYKERFPKATAQMEERLAEFIASNAPENVLQLADGVLSFIHHQVIELARDCLDKSREGLITSRYFYELQENLEKLLQDAHERSESSEVAFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIISQLGLTRDPLEEMAQLNSYDSPDTPETDDSVESRGASVQSKKTPSEEEFETIKLISNGAYGAVYLVRHKTTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVISDEIAWPEGDDALPPDAQDLISKLLRQNPLERMGTGSAFEVKQHRFFKDLDWNGLLRQKAEFIPQLESEDDTSYFDTRSERYQHLDSEEEEDTNDDDHVEIRQFSSCSPRFSKVYSSMERLSIHEERKTPPPTKRSLSEEKDDRLDSLGGLKSRDRSWVIGSPEILRKRLSMSESSHTESDSSPPLTVRRRCSGLLDMPRFAISTEDEGTVLKRPQSEGMLLSTVQSREGLPVPIPEQPVEQELQLEGDAGPTTPSTAVSSASTLTAGSTADFSDPRARSNSNEGPDFTTPKAISDLAVRRARHRLLSGESGEKRTSRPVNKVIKSASATALSLLIPSDHHTCSPLASPMSPHSLSSNPSSRDSSPSRDFSPAIANLKPPIIIHRAGKKYGFTLRAIRVYMGDSDIYTVHHMVWHVEEGGPANEAGLREGDLITHVNGEPVHGLVHTEVVELILKSGNKVSISTTPFENTSIKVGPARKASYKSKMARRNKKSKTKDGQESKKKSSLLRKITKQASLLHTSRSLSSLNRSLSSGESVPGSPTHNLSPRSPTQSYRSTPESVHSVGGNSSQSSSPSSSVPNSPASSGHIRPSSLHGLAPKLQRQYRSPRRKSAGNIPLSPLAHTPSPTPQSTSPQRSPSPLPGHSVGSSSIIQSFPVKLHSSPPLVRQISRPKSAEPPRSPLLKRVQSAEKLAASLSSSEKKLGSSRKHSLDISHSEFKKEMLQRDPSLQSLQESANETTGGKLGLAEKGMLQKPGSRKLGAIRQDRVERRESLQKQEAIREVDSSEDETDDGSEDSQDGRRLDKPPDSGDQGSDSFNEDNKFSSKLESKDSIEDDAFLPEDPKGTEDLQKGNSQQAKTISEPLQISVHPSPSEVPPRTSPEKLGNSEKPFLSSETTAKEHKLPENKTFECLGPKDKSSEAIKDLGRTASTQKPVDRTEHVQCPSIKLVELEEGDSLGASCGKLDLKDPLLTDSSQKKQDSKPLLALSSWCTASVSPPLSVSPADRSEKDHKEPLQPAEQHSTSFLPPGSVSETSQKSPSTEKQPSSSQAESASAASKATSPAAPVPSTLLVPAAIERALSVSQLVPLAQSVLGPLKLSMSGSGEVEKRKDFPHLGASCKEERDSKQKKQETSQGGGACQEKAKLSSTGGLATRSRSCTESLNSATCPVVAKKEAAFCSPKASEVLGGSCKTTPSKELSHALGQGALRASPLPDGSTRPSKEGTLAQAKSKEVGNQLKIQDFPLSHDGAVKKT

Target ORF information:

RefSeq Version	XM_014939194.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microtubule associated serine/threonine kinase 2 (MAST2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939194.1

ORF Insert Sequence:

ATGATAACAG AGTCCACTCC ATTACGCTGC AGGAAGCTCA GCAATCCTGA CATCTTTTCA 
TCTGCTGGGA AAACCAAGCT CCATCGTCAG CTAAGTCAAG ATGACTGCAA GCTACGAAGA 
GGTAGTTTGG CAAGTTCTTT GTCAGGAAAA CAGCTACTTC CCTTATCAAA TAGTGTCCAC 
AGTGGAGTGG GACAAACAAT ATGGCAGCCA CCTGGAGATA CCTCAAACCT GGTCCGCATG 
AGAAATCAGT CCCTTGGACA ATCTGCTCCT TCACTTACTG CTGGTTTGAA GGAGCTCAGT 
CTCCCCAGAA GAGGAAGCTT CTGTCGGACA AGCAACAGAA AGAGTTTGAT CGTAACATCC 
AGCACATCTC CAACTCTCCC GCGACCACAT TCCCCTCTTC ATGGACACGC AGGTAGCAGC 
CCTTTGGACA GTCCCAGGAA CTTCTCTCCA AATGCACCTG CTCATTTTTC CTTTGTTCCT 
GCCCGAAGAA CGGATGGGCG CCGCTGGTCC TTGGCCTCCC TTCCTTCATC TGGATATGGA 
ACAAACACTC CCAGTTCAAC AGTTTCATCA TCATGCTCAT CTCAGGAGAA GCTGCACCAG 
CTGCCCTTTC AGCCCACGGC AGATGAACTT CATTTCCTGA CAAAGCATTT CAGTACTGAG 
AGCATCACTG ATGAGGAAGG GCGCCATTCT CCAGCCTTGA GGCCTCGATC TCGCAGTCTC 
AGCCCGGGTC GCTCCCCAAT TTCCTTTGAC AGTGAAATAA TAATGATGAA TCATGTGTAC 
AAAGAAAGGT TTCCAAAGGC CACGGCGCAG ATGGAAGAAC GGCTGGCTGA GTTTATTGCC 
TCTAATGCTC CAGAGAACGT GCTGCAATTA GCAGATGGGG TCCTAAGTTT CATTCATCAT 
CAAGTTATTG AACTGGCCAG AGATTGCCTA GATAAATCAC GTGAAGGTCT TATTACTTCT 
CGGTACTTTT ATGAGCTGCA GGAAAACTTG GAGAAACTTC TCCAGGATGC CCACGAGCGA 
TCCGAGAGCT CCGAGGTTGC CTTCGTTACC CAACTTGTGA AGAAGCTGAT GATTATCATT 
GCACGGCCGG CTCGGCTGCT GGAGTGCCTG GAATTTGATC CTGAAGAGTT CTACCATTTG 
CTAGAAGCTG CAGAAGGCCA TGCCAAGGAA GGGCAAGGAA TTAAATGTGA CATTCCTCGT 
TATATTATCA GCCAGTTGGG ACTCACCAGG GATCCCCTGG AGGAAATGGC CCAGCTGAAT 
AGCTATGACA GCCCAGACAC TCCTGAGACA GATGATTCAG TCGAGAGCCG TGGGGCCTCT 
GTCCAGTCAA AGAAAACTCC ATCTGAAGAA GAGTTTGAAA CCATTAAACT GATCAGTAAT 
GGTGCTTATG GAGCGGTGTA TTTGGTGCGA CACAAGACCA CCCGTCAACG TTTTGCCATG 
AAGAAGATCA ACAAACAGAA CCTAATTTTG AGAAACCAGA TCCAGCAGGC TTTTGTGGAG 
AGAGATATCT TGACGTTTGC TGAGAACCCC TTCGTGGTCA GCATGTTCTG CTCCTTTGAG 
ACCAAGCGGC ACCTGTGCAT GGTTATGGAG TATGTGGAAG GAGGTGATTG TGCCACCCTT 
CTCAAGAACA TCGGTGCCCT GCCTGTTGAT ATGGCGAGGA TGTATTTTGC TGAGACTGTT 
CTGGCATTGG AGTACCTACA CAATTATGGG ATTGTTCACC GTGACCTAAA ACCTGATAAT 
CTCCTGATAA CTTCCATGGG TCACATTAAA CTAACAGACT TTGGACTGTC TAAAATTGGG 
TTAATGAGTC TTACAACTAA CCTTTATGAG GGACACATTG AGAAGGATAC CAGGGAGTTC 
CTGGACAAGC AGGTCTGTGG GACTCCAGAA TACATCGCGC CAGAAGTGAT CCTGCGCCAG 
GGCTATGGGA AGCCCGTGGA CTGGTGGGCC ATGGGAGTTA TCTTGTACGA GTTTCTGGTC 
GGCTGCGTCC CTTTCTTTGG GGACACACCT GAAGAGCTGT TTGGACAAGT GATCAGTGAT 
GAAATTGCCT GGCCAGAAGG TGATGATGCA CTGCCGCCAG ATGCCCAGGA CCTCATTTCT 
AAGCTTCTCC GCCAAAATCC TCTGGAAAGA ATGGGAACAG GTAGTGCCTT TGAAGTGAAA 
CAGCATCGGT TCTTTAAAGA TTTGGACTGG AATGGATTAC TTCGGCAGAA AGCTGAATTC 
ATCCCACAGT TGGAATCTGA GGATGACACA AGCTATTTCG ACACCCGTTC AGAACGGTAC 
CAACACCTGG ATTCAGAAGA AGAGGAGGAC ACTAATGATG ATGATCATGT GGAAATTCGG 
CAATTTTCCT CATGCTCACC AAGGTTCAGC AAGGTATACA GCAGCATGGA ACGTCTTTCC 
ATCCACGAAG AGAGGAAGAC TCCACCACCA ACTAAACGGA GTCTGAGTGA AGAAAAAGAT 
GACCGGCTGG ACAGCCTTGG TGGCTTGAAG AGTCGAGACC GCAGCTGGGT GATTGGGTCT 
CCTGAAATAC TGCGGAAGCG CTTGTCTATG TCTGAATCCT CGCACACGGA GAGTGACTCC 
AGCCCACCCT TGACCGTCCG CCGGCGCTGC TCAGGGCTCC TCGATATGCC CCGTTTTGCC 
ATCTCCACTG AGGACGAGGG AACTGTTCTC AAAAGGCCAC AGTCCGAGGG GATGCTCTTA 
TCCACCGTGC AGTCCCGGGA GGGGCTGCCA GTGCCCATTC CAGAGCAGCC TGTGGAGCAA 
GAGCTGCAGC TGGAAGGTGA TGCTGGACCC ACCACCCCCT CCACAGCAGT CAGCAGCGCC 
TCAACGCTGA CAGCTGGGAG CACTGCAGAT TTCTCTGATC CACGCGCTCG CAGTAATAGC 
AATGAAGGCC CAGACTTTAC CACTCCAAAA GCCATCAGCG ATTTGGCAGT GCGGCGGGCA 
CGACACAGGT TGCTCTCTGG GGAATCAGGG GAAAAACGTA CCTCGAGACC TGTCAATAAA 
GTGATTAAAT CGGCATCCGC TACTGCCTTG TCTCTCCTGA TTCCCTCAGA TCACCACACG 
TGTTCTCCAT TGGCCAGTCC AATGTCACCT CATTCTCTAT CCTCCAACCC ATCTTCGAGG 
GACTCCTCAC CCAGCCGCGA CTTTTCTCCT GCTATCGCAA ACCTGAAACC ACCAATCATC 
ATCCATCGGG CTGGAAAGAA ATACGGTTTC ACTCTCCGTG CCATTCGCGT CTATATGGGT 
GACAGTGATA TCTACACTGT GCATCACATG GTCTGGCACG TGGAGGAAGG TGGTCCAGCA 
AACGAAGCAG GTCTGCGTGA AGGGGATCTG ATAACCCATG TCAACGGGGA GCCGGTCCAT 
GGACTGGTGC ACACAGAAGT GGTGGAACTG ATTCTGAAGA GTGGAAATAA AGTGTCCATC 
TCCACCACGC CGTTTGAGAA CACCTCCATC AAAGTTGGTC CAGCTCGAAA AGCCAGCTAC 
AAATCCAAGA TGGCTCGTAG GAACAAGAAG AGCAAAACTA AGGATGGTCA GGAAAGTAAG 
AAGAAGAGCT CTTTGTTGAG GAAGATCACT AAACAGGCAT CACTACTTCA CACCAGCCGG 
AGTTTATCTT CACTAAACCG CTCTCTGTCT TCTGGAGAAA GCGTGCCTGG CTCTCCAACT 
CACAACCTGT CTCCTCGGTC ACCAACCCAG AGCTACAGAT CCACTCCCGA GTCTGTACAC 
TCAGTTGGTG GCAATTCTTC CCAGAGCAGC TCTCCCAGTT CCAGTGTTCC CAATTCTCCA 
GCCAGCTCTG GACACATCCG ACCCAGCTCT CTGCACGGAT TAGCACCGAA GCTTCAAAGG 
CAGTACAGGT CTCCGAGGCG CAAATCTGCG GGGAACATCC CTCTGTCACC CCTGGCTCAC 
ACTCCCTCAC CCACCCCACA GTCCACCTCA CCGCAGCGCT CCCCATCTCC TTTACCAGGA 
CACTCGGTTG GCAGCTCCAG TATCATCCAG TCTTTTCCAG TAAAACTACA CTCCTCTCCA 
CCGCTGGTGA GACAAATTTC TCGCCCCAAA AGCGCTGAGC CCCCTCGGTC TCCTTTACTA 
AAGAGAGTGC AGTCAGCTGA GAAGCTGGCT GCCTCGCTGT CTTCTTCTGA GAAGAAGCTG 
GGCTCCTCGC GTAAGCACAG CCTGGATATC TCTCACTCAG AATTTAAGAA GGAGATGCTC 
CAGAGGGATC CCAGTCTCCA GAGTTTACAA GAATCCGCGA ATGAAACAAC GGGTGGCAAA 
CTTGGGCTGG CAGAAAAAGG GATGTTGCAG AAGCCGGGTT CACGGAAGTT GGGTGCTATT 
CGACAGGACA GGGTGGAGAG GAGGGAGTCT CTGCAAAAGC AAGAGGCCAT CAGGGAAGTA 
GATTCTTCCG AAGATGAAAC GGATGATGGT TCAGAAGATA GTCAGGATGG GAGAAGACTA 
GACAAGCCCC CTGACAGCGG AGACCAAGGC TCAGATTCTT TTAATGAGGA TAATAAGTTT 
TCATCTAAAT TGGAAAGCAA GGATAGTATC GAAGATGATG CCTTTCTACC TGAAGATCCA 
AAAGGTACGG AAGATTTGCA GAAGGGAAAC AGTCAACAAG CCAAGACTAT TTCAGAGCCG 
CTGCAAATCA GTGTGCACCC TTCCCCATCA GAGGTCCCCC CAAGAACTTC TCCAGAAAAA 
TTAGGTAATT CAGAAAAGCC ATTCCTGAGC TCAGAAACAA CTGCAAAGGA ACATAAATTG 
CCAGAAAACA AAACCTTTGA GTGTCTTGGT CCAAAAGACA AGTCTTCTGA AGCAATCAAA 
GACCTAGGGA GAACTGCAAG TACCCAAAAA CCAGTAGACC GCACAGAGCA TGTACAGTGC 
CCGTCTATCA AACTCGTGGA ACTTGAAGAA GGTGACTCTT TGGGGGCCTC GTGTGGTAAG 
CTTGACCTGA AAGACCCTCT GCTAACAGAC AGCAGTCAGA AAAAACAGGA TTCCAAACCT 
CTGCTGGCAC TTTCTTCGTG GTGCACGGCG AGCGTGAGCC CTCCCCTCTC GGTGAGCCCT 
GCGGATCGCA GTGAGAAGGA TCACAAGGAG CCGCTTCAGC CTGCAGAACA GCACAGCACC 
TCCTTTCTGC CTCCTGGAAG CGTGAGTGAA ACCTCCCAAA AAAGTCCTAG TACTGAAAAG 
CAGCCCAGCT CGTCCCAGGC GGAGAGCGCT TCGGCCGCCA GCAAAGCGAC CAGCCCGGCT 
GCTCCTGTCC CCTCCACGCT CCTCGTCCCC GCTGCCATCG AAAGAGCTCT CTCCGTGTCC 
CAGTTAGTGC CACTCGCTCA GAGCGTGTTA GGCCCTTTGA AGCTCAGTAT GTCTGGTTCC 
GGAGAGGTGG AAAAGAGAAA GGATTTCCCC CATTTGGGTG CCTCCTGTAA AGAAGAGAGA 
GATTCCAAAC AAAAAAAGCA GGAAACTTCT CAAGGAGGAG GAGCGTGTCA AGAGAAAGCC 
AAACTATCTA GCACAGGCGG TCTGGCCACC AGGAGCAGGT CCTGCACAGA GTCGTTAAAC 
TCGGCCACGT GTCCCGTGGT GGCAAAAAAG GAGGCAGCTT TTTGCAGCCC TAAAGCATCT 
GAGGTGTTGG GAGGTAGCTG CAAAACCACA CCATCGAAAG AGCTGTCTCA CGCTCTTGGA 
CAAGGAGCTC TGAGAGCCTC TCCTCTGCCA GATGGCAGCA CGAGGCCCTC TAAAGAAGGG 
ACTCTGGCGC AGGCAAAAAG CAAAGAGGTT GGAAATCAGT TAAAAATACA AGACTTTCCC 
CTGTCACATG ATGGTGCTGT AAAGAAAACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MAST2 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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