MCF2L2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCF2L2
Entrez Gene ID	106886715
Full Name	MCF.2 cell line derived transforming sequence-like 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014939880.1	XP_014795366.1	probable guanine nucleotide exchange factor MCF2L2 isoform X1
XM_014939884.1	XP_014795370.1	probable guanine nucleotide exchange factor MCF2L2 isoform X5
XM_014939883.1	XP_014795369.1	probable guanine nucleotide exchange factor MCF2L2 isoform X4
XM_014939881.1	XP_014795367.1	probable guanine nucleotide exchange factor MCF2L2 isoform X2
XM_014939886.1	XP_014795372.1	probable guanine nucleotide exchange factor MCF2L2 isoform X7
XM_014939882.1	XP_014795368.1	probable guanine nucleotide exchange factor MCF2L2 isoform X3
XM_014939885.1	XP_014795371.1	probable guanine nucleotide exchange factor MCF2L2 isoform X6

Pathways from BioSystems
Gene Ontology

The following MCF2L2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCF2L2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa63495	XM_014939880.1 Latest version!	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa63496	XM_014939881.1 Latest version!	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa63497	XM_014939882.1 Latest version!	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa63498	XM_014939883.1 Latest version!	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa63499	XM_014939884.1 Latest version!	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa63500	XM_014939885.1 Latest version!	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa63501	XM_014939886.1 Latest version!	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCF2L2 ( XM_014939880.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795366.1 Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa63495
Clone ID Related Accession (Same CDS sequence)	XM_014939880.1
Accession Version	XM_014939880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable guanine nucleotide exchange factor MCF2L2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGGTAC CGATCATCAT GTTGAATTCC GTCTCCGACC TGCACGGCTA CATCGACAAA 
AGCCAGCTGA CGCGGGAGCT GGGGGGAACC CTGGAGTATG GGCACAGCCA GTGGATCCAC 
CACCGCACGG CTATTGAAAA CTTTGCAATG ACAGTAAAAA CAACAGCACA GATGCTGCAG 
AGCTTTGGAA CAGACCTCGC AGAGACTGAA CTTCCCAACG ACGTGCAGTG CACGGAGGAG 
CTGCTCTCCG CACACACAGA CCACCACGGC AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG 
AAGCAGGGGG CCACCCTGCT GACCTGCATC AGGGAGCCGG TCACCCGCAG CGCCAACAGC 
AAGCTCAGCC CTGACGAACT GGAGAACGTG GCAACAGTAG AGAGGTTACT GGCTCAGTTG 
GATGAAACGG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTGGAACAG 
TGCCTGCAGC TGCGACACTT TGAGCATGAT TTCAGAGAGG TAAAACTGGC ATTGGATAAT 
CTCATGGAAG GACAAGCAGG TTTTGCTGAC ATTGGAGACA GCGTGACTCG AGTGGAGCAT 
CTCCTCCGGG AACAGAAACA CCTGGAAGAG AAAGGACAGG AACCTTTGGA GAAGGCCCAG 
TCTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAATC ATTATGCAGT TGACTCCATT 
AGACCAAAGT GTGTTGAACT CAGGCGTATT TGTGATGACT TTACCAATGA GACCAAGAAA 
AAGTATGATA TTTTGGGAAA GTCTCTTGAG CTGCACAAGC AGTTAGATAA GGCAAGCCAA 
TGGTGTGAAG CTGGAATCTA CCTCTTGGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA 
GAGGGAGCTG AAACTGCACT CGTTGAGATT GAGAAGTTCC TGGTAACAGC CAAGGAGCAC 
CAGCTCTCCA ACCCGAAGGA GTTCTACAAT CAGTTTGACA TGATCCTCAC CCCTGAAATA 
AAGGCCAACG CCCAAAGAAT TCTACAGAAA CTAGAAGATG TACAAGAAAT GTTTGACAAG 
AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC AAGCAGACGC GGCCAGTGCA GCCTGTGGCC 
CCGCACCCCG AATCTTCCCC AAAGCGAGCC TCACCAAAGA TCACCAGACC AGCAGGAGTA 
GCTGCCAACA GGAGATCCAC AGAAATTTCC TGTCCTCCCA AGTCCATCTC TGAAGTCGAG 
TTATCAAAAA AGAAAAACAT TAAGAAGACT AAAGGTGGAA TTAAGATCGA AGTGATGCAC 
GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG CTGGTGATGA GCGAGAGCGA CGAGAACCTC 
TCCTCCAGGA GGAGCCACAT CATCAATGAG CTGATAGAGA CAGAACGGGT TTACGTAGAA 
GAACTTCAAA GTATAATAGA GGGATACGCC TCGGAAATGG ACAATCCCAG TTTGGTACAT 
CTGATCCCAG CTGCCCTCCA GAACAAGAAG GACATTCTTT TTGGGAACCT CCCAGAGATC 
TATGAATTCC ACAACAGGAT TTTCCTTAAG GAGATCGAAA ACTGCATTGA AAACCCTGAG 
CTTCTTGCCC GATGCTTTTT GAAAAGAAAG GAGGACCTCC AGATTTATGA GAAATACTGT 
CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTCCAG 
GAATGCCAGC GTAAATTGGA CCATAAGCTC TCCTTAGACG CTTATCTCTT AAAGCCGGTG 
CAGAGAATCA CCAAGTACCA GCTGCTGCTA AAGGAAATGC TGAAGTGCAG CAAGAATTCG 
GAGGGTACTG CTGAGCTGGA AGAAGCCCTG GCCACGATGC TTGATATCAT CAAGTCCGTG 
AACGACTCCA TGCACCAGAT AGCCATCACA GGATACGAGG GGGACGTTGG TGAGCTTGGC 
AAGTTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG GCACAACAAA 
GTGAAGGACT TGGCTAGGTT CAAACCCATG CAGAGACACC TCTTCCTCTA CACAAAGATG 
CTACTTTTCT GCAAGAAACG GGAGGAGAAC ACAGACGGAC ATGAGAAGAC AGCTTCATAC 
AGCTTTAAAA ATTCTTTAAA GATGAGCACT GTGGGCATTA CAGAAAATGT AAAAGGGGAT 
AACAAGAAAT TTGAGATCTG GTATAATGGC AGAGAAGAGG TCTATATCAT TCAGGCATCT 
TCTGTTGAGC TGAAGAACAC CTGGATTTCA GAGATTCGCA AAGTCCTGAC AGGACAACTG 
GAAGCGTGTA GAGAAGCAAG TCAACTGCAC CAAAGGATCA CCGAGAGCGT ATACCACGCA 
CCTATGGACT CCTCCAACCC AAGCAGGTAT AGCAGGAAGT CATCGAGTCT GTATGAAAAC 
AAGTCCGAGA CATCAAACGT GGAAGCATCT AACAACAAAA ACAGGAATTC CAGTCCCAGC 
GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT 
CGAAAGCGGT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCGGAG 
TGTAAGGAGA GAGAAGTCAC CCGTCGCTTT TCCCTTGCCT CCTCCATCAG CTCTGCAGCT 
AACGCCTCCG CTGGCACCAA AGGTCCCCTT GTTCCTGCAG TTAAAGTCAA GAGGCATGAA 
ATAAAGAGTG ACCCAACTCC CCTGGGGTTT GAAGATGCTT TTGAGAACAC CATTTTGGCT 
CAGACTAAAT GTAATACCGG TTCCACCACG AGGCCCGTCC CTAGATCTGC TTCACAGACA 
GGCTGGCCCG GCAGGCAGAT GCCATCTCTA GACACCTTTG AAGATTTTGA AGCCATCCCC 
TCCAGCACGG ACGAGCTCTC CAACTCCTCG GACTTGGAGG AAGAGACCAA CCCCAACAAC 
GGGACCAATC TCTTTCGGGT GGAAGGCAGC TTTGACAGCT GTTTCCCGGA TTCTCTGACC 
CTGGAGGATG GAGATTTAGT GCAGTTTGTG CAGGAAGGAG AGAATGGTCA ATGGCTGGTG 
AAGAAGCTGG TCTCTGAGAA GAGCGACTGG GTTCCCTCCA GTATCTTGCA GCCCGCAGAG 
GGGGACAGTC ACACCATCAT TAAGAACAGC AATGCAGACA CGTACAACGA TTCCTGCAGC 
ACGGCCTCCC TGGAGTCGGA CACCAAGGAG AATGAGGTGG CCAAGAGCTT CCCAGCGGAG 
CAGAGGGCCG GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795366.1
CDS	512..4369
Translation	MASRCRKCGQQVPRNPPFTAVRITEGEMQSYHDFIGGCIRLERMAKRDEIMQQEIRPLVAVDIIEQLHRQFAILSGGRGKDGAPIITFPEFAGFSDIPDEDFLNVVTYLTSIPSLEAASIGFIIIIDRRRDKWSAVKASLTRIAGAFPGNLQLVFVLRPSRFIQRTIADIGIKLYRDDFKMKVPIIMLNSVSDLHGYIDKSQLTRELGGTLEYGHSQWIHHRTAIENFAMTVKTTAQMLQSFGTDLAETELPNDVQCTEELLSAHTDHHGKLKDELKLAVKQGATLLTCIREPVTRSANSKLSPDELENVATVERLLAQLDETEKAFDQFWTKHHLKLEQCLQLRHFEHDFREVKLALDNLMEGQAGFADIGDSVTRVEHLLREQKHLEEKGQEPLEKAQSLALHGEQLIQNNHYAVDSIRPKCVELRRICDDFTNETKKKYDILGKSLELHKQLDKASQWCEAGIYLLASQAVDKCQSQEGAETALVEIEKFLVTAKEHQLSNPKEFYNQFDMILTPEIKANAQRILQKLEDVQEMFDKRQVSLKKLAAKQTRPVQPVAPHPESSPKRASPKITRPAGVAANRRSTEISCPPKSISEVELSKKKNIKKTKGGIKIEVMHEASQGGSSRVLVMSESDENLSSRRSHIINELIETERVYVEELQSIIEGYASEMDNPSLVHLIPAALQNKKDILFGNLPEIYEFHNRIFLKEIENCIENPELLARCFLKRKEDLQIYEKYCQNKPRSEALWRQCGDSIFFQECQRKLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATMLDIIKSVNDSMHQIAITGYEGDVGELGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYTKMLLFCKKREENTDGHEKTASYSFKNSLKMSTVGITENVKGDNKKFEIWYNGREEVYIIQASSVELKNTWISEIRKVLTGQLEACREASQLHQRITESVYHAPMDSSNPSRYSRKSSSLYENKSETSNVEASNNKNRNSSPSARQKRVDASTSLNLERTALARKRFTLQGLSNRRAPPKDPECKEREVTRRFSLASSISSAANASAGTKGPLVPAVKVKRHEIKSDPTPLGFEDAFENTILAQTKCNTGSTTRPVPRSASQTGWPGRQMPSLDTFEDFEAIPSSTDELSNSSDLEEETNPNNGTNLFRVEGSFDSCFPDSLTLEDGDLVQFVQEGENGQWLVKKLVSEKSDWVPSSILQPAEGDSHTIIKNSNADTYNDSCSTASLESDTKENEVAKSFPAEQRAGS

Target ORF information:

RefSeq Version	XM_014939880.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939880.1

ORF Insert Sequence:

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGGTAC CGATCATCAT GTTGAATTCC GTCTCCGACC TGCACGGCTA CATCGACAAA 
AGCCAGCTGA CGCGGGAGCT GGGGGGAACC CTGGAGTATG GGCACAGCCA GTGGATCCAC 
CACCGCACGG CTATTGAAAA CTTTGCAATG ACAGTAAAAA CAACAGCACA GATGCTGCAG 
AGCTTTGGAA CAGACCTCGC AGAGACTGAA CTTCCCAACG ACGTGCAGTG CACGGAGGAG 
CTGCTCTCCG CACACACAGA CCACCACGGC AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG 
AAGCAGGGGG CCACCCTGCT GACCTGCATC AGGGAGCCGG TCACCCGCAG CGCCAACAGC 
AAGCTCAGCC CTGACGAACT GGAGAACGTG GCAACAGTAG AGAGGTTACT GGCTCAGTTG 
GATGAAACGG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTGGAACAG 
TGCCTGCAGC TGCGACACTT TGAGCATGAT TTCAGAGAGG TAAAACTGGC ATTGGATAAT 
CTCATGGAAG GACAAGCAGG TTTTGCTGAC ATTGGAGACA GCGTGACTCG AGTGGAGCAT 
CTCCTCCGGG AACAGAAACA CCTGGAAGAG AAAGGACAGG AACCTTTGGA GAAGGCCCAG 
TCTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAATC ATTATGCAGT TGACTCCATT 
AGACCAAAGT GTGTTGAACT CAGGCGTATT TGTGATGACT TTACCAATGA GACCAAGAAA 
AAGTATGATA TTTTGGGAAA GTCTCTTGAG CTGCACAAGC AGTTAGATAA GGCAAGCCAA 
TGGTGTGAAG CTGGAATCTA CCTCTTGGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA 
GAGGGAGCTG AAACTGCACT CGTTGAGATT GAGAAGTTCC TGGTAACAGC CAAGGAGCAC 
CAGCTCTCCA ACCCGAAGGA GTTCTACAAT CAGTTTGACA TGATCCTCAC CCCTGAAATA 
AAGGCCAACG CCCAAAGAAT TCTACAGAAA CTAGAAGATG TACAAGAAAT GTTTGACAAG 
AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC AAGCAGACGC GGCCAGTGCA GCCTGTGGCC 
CCGCACCCCG AATCTTCCCC AAAGCGAGCC TCACCAAAGA TCACCAGACC AGCAGGAGTA 
GCTGCCAACA GGAGATCCAC AGAAATTTCC TGTCCTCCCA AGTCCATCTC TGAAGTCGAG 
TTATCAAAAA AGAAAAACAT TAAGAAGACT AAAGGTGGAA TTAAGATCGA AGTGATGCAC 
GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG CTGGTGATGA GCGAGAGCGA CGAGAACCTC 
TCCTCCAGGA GGAGCCACAT CATCAATGAG CTGATAGAGA CAGAACGGGT TTACGTAGAA 
GAACTTCAAA GTATAATAGA GGGATACGCC TCGGAAATGG ACAATCCCAG TTTGGTACAT 
CTGATCCCAG CTGCCCTCCA GAACAAGAAG GACATTCTTT TTGGGAACCT CCCAGAGATC 
TATGAATTCC ACAACAGGAT TTTCCTTAAG GAGATCGAAA ACTGCATTGA AAACCCTGAG 
CTTCTTGCCC GATGCTTTTT GAAAAGAAAG GAGGACCTCC AGATTTATGA GAAATACTGT 
CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTCCAG 
GAATGCCAGC GTAAATTGGA CCATAAGCTC TCCTTAGACG CTTATCTCTT AAAGCCGGTG 
CAGAGAATCA CCAAGTACCA GCTGCTGCTA AAGGAAATGC TGAAGTGCAG CAAGAATTCG 
GAGGGTACTG CTGAGCTGGA AGAAGCCCTG GCCACGATGC TTGATATCAT CAAGTCCGTG 
AACGACTCCA TGCACCAGAT AGCCATCACA GGATACGAGG GGGACGTTGG TGAGCTTGGC 
AAGTTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG GCACAACAAA 
GTGAAGGACT TGGCTAGGTT CAAACCCATG CAGAGACACC TCTTCCTCTA CACAAAGATG 
CTACTTTTCT GCAAGAAACG GGAGGAGAAC ACAGACGGAC ATGAGAAGAC AGCTTCATAC 
AGCTTTAAAA ATTCTTTAAA GATGAGCACT GTGGGCATTA CAGAAAATGT AAAAGGGGAT 
AACAAGAAAT TTGAGATCTG GTATAATGGC AGAGAAGAGG TCTATATCAT TCAGGCATCT 
TCTGTTGAGC TGAAGAACAC CTGGATTTCA GAGATTCGCA AAGTCCTGAC AGGACAACTG 
GAAGCGTGTA GAGAAGCAAG TCAACTGCAC CAAAGGATCA CCGAGAGCGT ATACCACGCA 
CCTATGGACT CCTCCAACCC AAGCAGGTAT AGCAGGAAGT CATCGAGTCT GTATGAAAAC 
AAGTCCGAGA CATCAAACGT GGAAGCATCT AACAACAAAA ACAGGAATTC CAGTCCCAGC 
GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT 
CGAAAGCGGT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCGGAG 
TGTAAGGAGA GAGAAGTCAC CCGTCGCTTT TCCCTTGCCT CCTCCATCAG CTCTGCAGCT 
AACGCCTCCG CTGGCACCAA AGGTCCCCTT GTTCCTGCAG TTAAAGTCAA GAGGCATGAA 
ATAAAGAGTG ACCCAACTCC CCTGGGGTTT GAAGATGCTT TTGAGAACAC CATTTTGGCT 
CAGACTAAAT GTAATACCGG TTCCACCACG AGGCCCGTCC CTAGATCTGC TTCACAGACA 
GGCTGGCCCG GCAGGCAGAT GCCATCTCTA GACACCTTTG AAGATTTTGA AGCCATCCCC 
TCCAGCACGG ACGAGCTCTC CAACTCCTCG GACTTGGAGG AAGAGACCAA CCCCAACAAC 
GGGACCAATC TCTTTCGGGT GGAAGGCAGC TTTGACAGCT GTTTCCCGGA TTCTCTGACC 
CTGGAGGATG GAGATTTAGT GCAGTTTGTG CAGGAAGGAG AGAATGGTCA ATGGCTGGTG 
AAGAAGCTGG TCTCTGAGAA GAGCGACTGG GTTCCCTCCA GTATCTTGCA GCCCGCAGAG 
GGGGACAGTC ACACCATCAT TAAGAACAGC AATGCAGACA CGTACAACGA TTCCTGCAGC 
ACGGCCTCCC TGGAGTCGGA CACCAAGGAG AATGAGGTGG CCAAGAGCTT CCCAGCGGAG 
CAGAGGGCCG GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L2 ( XM_014939881.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795367.1 Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa63496
Clone ID Related Accession (Same CDS sequence)	XM_014939881.1
Accession Version	XM_014939881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable guanine nucleotide exchange factor MCF2L2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGATCA TCATGTTGAA TTCCGTCTCC GACCTGCACG GCTACATCGA CAAAAGCCAG 
CTGACGCGGG AGCTGGGGGG AACCCTGGAG TATGGGCACA GCCAGTGGAT CCACCACCGC 
ACGGCTATTG AAAACTTTGC AATGACAGTA AAAACAACAG CACAGATGCT GCAGAGCTTT 
GGAACAGACC TCGCAGAGAC TGAACTTCCC AACGACGTGC AGTGCACGGA GGAGCTGCTC 
TCCGCACACA CAGACCACCA CGGCAAGCTG AAGGACGAGC TGAAGCTGGC TGTGAAGCAG 
GGGGCCACCC TGCTGACCTG CATCAGGGAG CCGGTCACCC GCAGCGCCAA CAGCAAGCTC 
AGCCCTGACG AACTGGAGAA CGTGGCAACA GTAGAGAGGT TACTGGCTCA GTTGGATGAA 
ACGGAAAAGG CTTTTGATCA ATTTTGGACC AAGCATCACC TAAAACTGGA ACAGTGCCTG 
CAGCTGCGAC ACTTTGAGCA TGATTTCAGA GAGGTAAAAC TGGCATTGGA TAATCTCATG 
GAAGGACAAG CAGGTTTTGC TGACATTGGA GACAGCGTGA CTCGAGTGGA GCATCTCCTC 
CGGGAACAGA AACACCTGGA AGAGAAAGGA CAGGAACCTT TGGAGAAGGC CCAGTCTCTA 
GCTCTCCATG GAGAACAGCT CATCCAAAAC AATCATTATG CAGTTGACTC CATTAGACCA 
AAGTGTGTTG AACTCAGGCG TATTTGTGAT GACTTTACCA ATGAGACCAA GAAAAAGTAT 
GATATTTTGG GAAAGTCTCT TGAGCTGCAC AAGCAGTTAG ATAAGGCAAG CCAATGGTGT 
GAAGCTGGAA TCTACCTCTT GGCTTCCCAA GCTGTGGACA AGTGCCAGTC ACAAGAGGGA 
GCTGAAACTG CACTCGTTGA GATTGAGAAG TTCCTGGTAA CAGCCAAGGA GCACCAGCTC 
TCCAACCCGA AGGAGTTCTA CAATCAGTTT GACATGATCC TCACCCCTGA AATAAAGGCC 
AACGCCCAAA GAATTCTACA GAAACTAGAA GATGTACAAG AAATGTTTGA CAAGAGGCAA 
GTGAGCCTGA AGAAGCTGGC AGCCAAGCAG ACGCGGCCAG TGCAGCCTGT GGCCCCGCAC 
CCCGAATCTT CCCCAAAGCG AGCCTCACCA AAGATCACCA GACCAGCAGG AGTAGCTGCC 
AACAGGAGAT CCACAGAAAT TTCCTGTCCT CCCAAGTCCA TCTCTGAAGT CGAGTTATCA 
AAAAAGAAAA ACATTAAGAA GACTAAAGGT GGAATTAAGA TCGAAGTGAT GCACGAGGCC 
AGCCAGGGAG GGTCCAGCAG GGTGCTGGTG ATGAGCGAGA GCGACGAGAA CCTCTCCTCC 
AGGAGGAGCC ACATCATCAA TGAGCTGATA GAGACAGAAC GGGTTTACGT AGAAGAACTT 
CAAAGTATAA TAGAGGGATA CGCCTCGGAA ATGGACAATC CCAGTTTGGT ACATCTGATC 
CCAGCTGCCC TCCAGAACAA GAAGGACATT CTTTTTGGGA ACCTCCCAGA GATCTATGAA 
TTCCACAACA GGATTTTCCT TAAGGAGATC GAAAACTGCA TTGAAAACCC TGAGCTTCTT 
GCCCGATGCT TTTTGAAAAG AAAGGAGGAC CTCCAGATTT ATGAGAAATA CTGTCAGAAC 
AAGCCAAGGT CTGAAGCTCT CTGGAGACAG TGTGGAGACA GCATCTTTTT CCAGGAATGC 
CAGCGTAAAT TGGACCATAA GCTCTCCTTA GACGCTTATC TCTTAAAGCC GGTGCAGAGA 
ATCACCAAGT ACCAGCTGCT GCTAAAGGAA ATGCTGAAGT GCAGCAAGAA TTCGGAGGGT 
ACTGCTGAGC TGGAAGAAGC CCTGGCCACG ATGCTTGATA TCATCAAGTC CGTGAACGAC 
TCCATGCACC AGATAGCCAT CACAGGATAC GAGGGGGACG TTGGTGAGCT TGGCAAGTTG 
TTGATGCAAG GTTCCTTCAA CGTGTGGACT GACCACAAGA AGGGGCACAA CAAAGTGAAG 
GACTTGGCTA GGTTCAAACC CATGCAGAGA CACCTCTTCC TCTACACAAA GATGCTACTT 
TTCTGCAAGA AACGGGAGGA GAACACAGAC GGACATGAGA AGACAGCTTC ATACAGCTTT 
AAAAATTCTT TAAAGATGAG CACTGTGGGC ATTACAGAAA ATGTAAAAGG GGATAACAAG 
AAATTTGAGA TCTGGTATAA TGGCAGAGAA GAGGTCTATA TCATTCAGGC ATCTTCTGTT 
GAGCTGAAGA ACACCTGGAT TTCAGAGATT CGCAAAGTCC TGACAGGACA ACTGGAAGCG 
TGTAGAGAAG CAAGTCAACT GCACCAAAGG ATCACCGAGA GCGTATACCA CGCACCTATG 
GACTCCTCCA ACCCAAGCAG GTATAGCAGG AAGTCATCGA GTCTGTATGA AAACAAGTCC 
GAGACATCAA ACGTGGAAGC ATCTAACAAC AAAAACAGGA ATTCCAGTCC CAGCGCCAGA 
CAAAAGAGAG TTGATGCTTC CACCAGCCTT AACCTTGAGC GCACAGCGCT TGCTCGAAAG 
CGGTTCACAT TGCAAGGTCT TTCCAACCGC AGAGCTCCAC CCAAAGATCC GGAGTGTAAG 
GAGAGAGAAG TCACCCGTCG CTTTTCCCTT GCCTCCTCCA TCAGCTCTGC AGCTAACGCC 
TCCGCTGGCA CCAAAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGGCA TGAAATAAAG 
AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATTTT GGCTCAGACT 
AAATGTAATA CCGGTTCCAC CACGAGGCCC GTCCCTAGAT CTGCTTCACA GACAGGCTGG 
CCCGGCAGGC AGATGCCATC TCTAGACACC TTTGAAGATT TTGAAGCCAT CCCCTCCAGC 
ACGGACGAGC TCTCCAACTC CTCGGACTTG GAGGAAGAGA CCAACCCCAA CAACGGGACC 
AATCTCTTTC GGGTGGAAGG CAGCTTTGAC AGCTGTTTCC CGGATTCTCT GACCCTGGAG 
GATGGAGATT TAGTGCAGTT TGTGCAGGAA GGAGAGAATG GTCAATGGCT GGTGAAGAAG 
CTGGTCTCTG AGAAGAGCGA CTGGGTTCCC TCCAGTATCT TGCAGCCCGC AGAGGGGGAC 
AGTCACACCA TCATTAAGAA CAGCAATGCA GACACGTACA ACGATTCCTG CAGCACGGCC 
TCCCTGGAGT CGGACACCAA GGAGAATGAG GTGGCCAAGA GCTTCCCAGC GGAGCAGAGG 
GCCGGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795367.1
CDS	512..4363
Translation	MASRCRKCGQQVPRNPPFTAVRITEGEMQSYHDFIGGCIRLERMAKRDEIMQQEIRPLVAVDIIEQLHRQFAILSGGRGKDGAPIITFPEFAGFSDIPDEDFLNVVTYLTSIPSLEAASIGFIIIIDRRRDKWSAVKASLTRIAGAFPGNLQLVFVLRPSRFIQRTIADIGIKLYRDDFKMKIIMLNSVSDLHGYIDKSQLTRELGGTLEYGHSQWIHHRTAIENFAMTVKTTAQMLQSFGTDLAETELPNDVQCTEELLSAHTDHHGKLKDELKLAVKQGATLLTCIREPVTRSANSKLSPDELENVATVERLLAQLDETEKAFDQFWTKHHLKLEQCLQLRHFEHDFREVKLALDNLMEGQAGFADIGDSVTRVEHLLREQKHLEEKGQEPLEKAQSLALHGEQLIQNNHYAVDSIRPKCVELRRICDDFTNETKKKYDILGKSLELHKQLDKASQWCEAGIYLLASQAVDKCQSQEGAETALVEIEKFLVTAKEHQLSNPKEFYNQFDMILTPEIKANAQRILQKLEDVQEMFDKRQVSLKKLAAKQTRPVQPVAPHPESSPKRASPKITRPAGVAANRRSTEISCPPKSISEVELSKKKNIKKTKGGIKIEVMHEASQGGSSRVLVMSESDENLSSRRSHIINELIETERVYVEELQSIIEGYASEMDNPSLVHLIPAALQNKKDILFGNLPEIYEFHNRIFLKEIENCIENPELLARCFLKRKEDLQIYEKYCQNKPRSEALWRQCGDSIFFQECQRKLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATMLDIIKSVNDSMHQIAITGYEGDVGELGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYTKMLLFCKKREENTDGHEKTASYSFKNSLKMSTVGITENVKGDNKKFEIWYNGREEVYIIQASSVELKNTWISEIRKVLTGQLEACREASQLHQRITESVYHAPMDSSNPSRYSRKSSSLYENKSETSNVEASNNKNRNSSPSARQKRVDASTSLNLERTALARKRFTLQGLSNRRAPPKDPECKEREVTRRFSLASSISSAANASAGTKGPLVPAVKVKRHEIKSDPTPLGFEDAFENTILAQTKCNTGSTTRPVPRSASQTGWPGRQMPSLDTFEDFEAIPSSTDELSNSSDLEEETNPNNGTNLFRVEGSFDSCFPDSLTLEDGDLVQFVQEGENGQWLVKKLVSEKSDWVPSSILQPAEGDSHTIIKNSNADTYNDSCSTASLESDTKENEVAKSFPAEQRAGS

Target ORF information:

RefSeq Version	XM_014939881.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939881.1

ORF Insert Sequence:

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGATCA TCATGTTGAA TTCCGTCTCC GACCTGCACG GCTACATCGA CAAAAGCCAG 
CTGACGCGGG AGCTGGGGGG AACCCTGGAG TATGGGCACA GCCAGTGGAT CCACCACCGC 
ACGGCTATTG AAAACTTTGC AATGACAGTA AAAACAACAG CACAGATGCT GCAGAGCTTT 
GGAACAGACC TCGCAGAGAC TGAACTTCCC AACGACGTGC AGTGCACGGA GGAGCTGCTC 
TCCGCACACA CAGACCACCA CGGCAAGCTG AAGGACGAGC TGAAGCTGGC TGTGAAGCAG 
GGGGCCACCC TGCTGACCTG CATCAGGGAG CCGGTCACCC GCAGCGCCAA CAGCAAGCTC 
AGCCCTGACG AACTGGAGAA CGTGGCAACA GTAGAGAGGT TACTGGCTCA GTTGGATGAA 
ACGGAAAAGG CTTTTGATCA ATTTTGGACC AAGCATCACC TAAAACTGGA ACAGTGCCTG 
CAGCTGCGAC ACTTTGAGCA TGATTTCAGA GAGGTAAAAC TGGCATTGGA TAATCTCATG 
GAAGGACAAG CAGGTTTTGC TGACATTGGA GACAGCGTGA CTCGAGTGGA GCATCTCCTC 
CGGGAACAGA AACACCTGGA AGAGAAAGGA CAGGAACCTT TGGAGAAGGC CCAGTCTCTA 
GCTCTCCATG GAGAACAGCT CATCCAAAAC AATCATTATG CAGTTGACTC CATTAGACCA 
AAGTGTGTTG AACTCAGGCG TATTTGTGAT GACTTTACCA ATGAGACCAA GAAAAAGTAT 
GATATTTTGG GAAAGTCTCT TGAGCTGCAC AAGCAGTTAG ATAAGGCAAG CCAATGGTGT 
GAAGCTGGAA TCTACCTCTT GGCTTCCCAA GCTGTGGACA AGTGCCAGTC ACAAGAGGGA 
GCTGAAACTG CACTCGTTGA GATTGAGAAG TTCCTGGTAA CAGCCAAGGA GCACCAGCTC 
TCCAACCCGA AGGAGTTCTA CAATCAGTTT GACATGATCC TCACCCCTGA AATAAAGGCC 
AACGCCCAAA GAATTCTACA GAAACTAGAA GATGTACAAG AAATGTTTGA CAAGAGGCAA 
GTGAGCCTGA AGAAGCTGGC AGCCAAGCAG ACGCGGCCAG TGCAGCCTGT GGCCCCGCAC 
CCCGAATCTT CCCCAAAGCG AGCCTCACCA AAGATCACCA GACCAGCAGG AGTAGCTGCC 
AACAGGAGAT CCACAGAAAT TTCCTGTCCT CCCAAGTCCA TCTCTGAAGT CGAGTTATCA 
AAAAAGAAAA ACATTAAGAA GACTAAAGGT GGAATTAAGA TCGAAGTGAT GCACGAGGCC 
AGCCAGGGAG GGTCCAGCAG GGTGCTGGTG ATGAGCGAGA GCGACGAGAA CCTCTCCTCC 
AGGAGGAGCC ACATCATCAA TGAGCTGATA GAGACAGAAC GGGTTTACGT AGAAGAACTT 
CAAAGTATAA TAGAGGGATA CGCCTCGGAA ATGGACAATC CCAGTTTGGT ACATCTGATC 
CCAGCTGCCC TCCAGAACAA GAAGGACATT CTTTTTGGGA ACCTCCCAGA GATCTATGAA 
TTCCACAACA GGATTTTCCT TAAGGAGATC GAAAACTGCA TTGAAAACCC TGAGCTTCTT 
GCCCGATGCT TTTTGAAAAG AAAGGAGGAC CTCCAGATTT ATGAGAAATA CTGTCAGAAC 
AAGCCAAGGT CTGAAGCTCT CTGGAGACAG TGTGGAGACA GCATCTTTTT CCAGGAATGC 
CAGCGTAAAT TGGACCATAA GCTCTCCTTA GACGCTTATC TCTTAAAGCC GGTGCAGAGA 
ATCACCAAGT ACCAGCTGCT GCTAAAGGAA ATGCTGAAGT GCAGCAAGAA TTCGGAGGGT 
ACTGCTGAGC TGGAAGAAGC CCTGGCCACG ATGCTTGATA TCATCAAGTC CGTGAACGAC 
TCCATGCACC AGATAGCCAT CACAGGATAC GAGGGGGACG TTGGTGAGCT TGGCAAGTTG 
TTGATGCAAG GTTCCTTCAA CGTGTGGACT GACCACAAGA AGGGGCACAA CAAAGTGAAG 
GACTTGGCTA GGTTCAAACC CATGCAGAGA CACCTCTTCC TCTACACAAA GATGCTACTT 
TTCTGCAAGA AACGGGAGGA GAACACAGAC GGACATGAGA AGACAGCTTC ATACAGCTTT 
AAAAATTCTT TAAAGATGAG CACTGTGGGC ATTACAGAAA ATGTAAAAGG GGATAACAAG 
AAATTTGAGA TCTGGTATAA TGGCAGAGAA GAGGTCTATA TCATTCAGGC ATCTTCTGTT 
GAGCTGAAGA ACACCTGGAT TTCAGAGATT CGCAAAGTCC TGACAGGACA ACTGGAAGCG 
TGTAGAGAAG CAAGTCAACT GCACCAAAGG ATCACCGAGA GCGTATACCA CGCACCTATG 
GACTCCTCCA ACCCAAGCAG GTATAGCAGG AAGTCATCGA GTCTGTATGA AAACAAGTCC 
GAGACATCAA ACGTGGAAGC ATCTAACAAC AAAAACAGGA ATTCCAGTCC CAGCGCCAGA 
CAAAAGAGAG TTGATGCTTC CACCAGCCTT AACCTTGAGC GCACAGCGCT TGCTCGAAAG 
CGGTTCACAT TGCAAGGTCT TTCCAACCGC AGAGCTCCAC CCAAAGATCC GGAGTGTAAG 
GAGAGAGAAG TCACCCGTCG CTTTTCCCTT GCCTCCTCCA TCAGCTCTGC AGCTAACGCC 
TCCGCTGGCA CCAAAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGGCA TGAAATAAAG 
AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATTTT GGCTCAGACT 
AAATGTAATA CCGGTTCCAC CACGAGGCCC GTCCCTAGAT CTGCTTCACA GACAGGCTGG 
CCCGGCAGGC AGATGCCATC TCTAGACACC TTTGAAGATT TTGAAGCCAT CCCCTCCAGC 
ACGGACGAGC TCTCCAACTC CTCGGACTTG GAGGAAGAGA CCAACCCCAA CAACGGGACC 
AATCTCTTTC GGGTGGAAGG CAGCTTTGAC AGCTGTTTCC CGGATTCTCT GACCCTGGAG 
GATGGAGATT TAGTGCAGTT TGTGCAGGAA GGAGAGAATG GTCAATGGCT GGTGAAGAAG 
CTGGTCTCTG AGAAGAGCGA CTGGGTTCCC TCCAGTATCT TGCAGCCCGC AGAGGGGGAC 
AGTCACACCA TCATTAAGAA CAGCAATGCA GACACGTACA ACGATTCCTG CAGCACGGCC 
TCCCTGGAGT CGGACACCAA GGAGAATGAG GTGGCCAAGA GCTTCCCAGC GGAGCAGAGG 
GCCGGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L2 ( XM_014939882.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795368.1 Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA.

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CloneID	OCa63497
Clone ID Related Accession (Same CDS sequence)	XM_014939882.1
Accession Version	XM_014939882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable guanine nucleotide exchange factor MCF2L2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCA GTTCCCTGTG TGAAATGCTT TTTTGGTTAA AAGAAGAAGA GATGTCACAT 
CAGGAGCTCA CCCAGCGACT GACTACGGTT ATCACCCATG TGGATGAAAT CATGCAGCAG 
GAGATACGGC CGCTGGTTGC AGTGGATATC ATAGAGCAGC TCCACAGGCA GTTTGCTATC 
TTGTCAGGAG GAAGAGGGAA GGATGGGGCG CCCATTATAA CCTTTCCAGA ATTTGCGGGA 
TTCAGTGACA TACCTGATGA AGATTTTCTG AACGTGGTCA CGTATCTCAC CAGCATCCCC 
AGTCTGGAGG CTGCCAGCAT CGGGTTCATC ATCATCATAG ACAGACGACG GGACAAATGG 
AGCGCAGTCA AGGCATCCCT GACACGGATA GCAGGAGCGT TTCCAGGGAA CCTGCAGCTG 
GTGTTTGTGC TGCGACCCTC CCGCTTCATC CAAAGGACCA TCGCTGACAT TGGCATTAAG 
CTCTATCGAG ATGACTTTAA AATGAAGGTA CCGATCATCA TGTTGAATTC CGTCTCCGAC 
CTGCACGGCT ACATCGACAA AAGCCAGCTG ACGCGGGAGC TGGGGGGAAC CCTGGAGTAT 
GGGCACAGCC AGTGGATCCA CCACCGCACG GCTATTGAAA ACTTTGCAAT GACAGTAAAA 
ACAACAGCAC AGATGCTGCA GAGCTTTGGA ACAGACCTCG CAGAGACTGA ACTTCCCAAC 
GACGTGCAGT GCACGGAGGA GCTGCTCTCC GCACACACAG ACCACCACGG CAAGCTGAAG 
GACGAGCTGA AGCTGGCTGT GAAGCAGGGG GCCACCCTGC TGACCTGCAT CAGGGAGCCG 
GTCACCCGCA GCGCCAACAG CAAGCTCAGC CCTGACGAAC TGGAGAACGT GGCAACAGTA 
GAGAGGTTAC TGGCTCAGTT GGATGAAACG GAAAAGGCTT TTGATCAATT TTGGACCAAG 
CATCACCTAA AACTGGAACA GTGCCTGCAG CTGCGACACT TTGAGCATGA TTTCAGAGAG 
GTAAAACTGG CATTGGATAA TCTCATGGAA GGACAAGCAG GTTTTGCTGA CATTGGAGAC 
AGCGTGACTC GAGTGGAGCA TCTCCTCCGG GAACAGAAAC ACCTGGAAGA GAAAGGACAG 
GAACCTTTGG AGAAGGCCCA GTCTCTAGCT CTCCATGGAG AACAGCTCAT CCAAAACAAT 
CATTATGCAG TTGACTCCAT TAGACCAAAG TGTGTTGAAC TCAGGCGTAT TTGTGATGAC 
TTTACCAATG AGACCAAGAA AAAGTATGAT ATTTTGGGAA AGTCTCTTGA GCTGCACAAG 
CAGTTAGATA AGGCAAGCCA ATGGTGTGAA GCTGGAATCT ACCTCTTGGC TTCCCAAGCT 
GTGGACAAGT GCCAGTCACA AGAGGGAGCT GAAACTGCAC TCGTTGAGAT TGAGAAGTTC 
CTGGTAACAG CCAAGGAGCA CCAGCTCTCC AACCCGAAGG AGTTCTACAA TCAGTTTGAC 
ATGATCCTCA CCCCTGAAAT AAAGGCCAAC GCCCAAAGAA TTCTACAGAA ACTAGAAGAT 
GTACAAGAAA TGTTTGACAA GAGGCAAGTG AGCCTGAAGA AGCTGGCAGC CAAGCAGACG 
CGGCCAGTGC AGCCTGTGGC CCCGCACCCC GAATCTTCCC CAAAGCGAGC CTCACCAAAG 
ATCACCAGAC CAGCAGGAGT AGCTGCCAAC AGGAGATCCA CAGAAATTTC CTGTCCTCCC 
AAGTCCATCT CTGAAGTCGA GTTATCAAAA AAGAAAAACA TTAAGAAGAC TAAAGGTGGA 
ATTAAGATCG AAGTGATGCA CGAGGCCAGC CAGGGAGGGT CCAGCAGGGT GCTGGTGATG 
AGCGAGAGCG ACGAGAACCT CTCCTCCAGG AGGAGCCACA TCATCAATGA GCTGATAGAG 
ACAGAACGGG TTTACGTAGA AGAACTTCAA AGTATAATAG AGGGATACGC CTCGGAAATG 
GACAATCCCA GTTTGGTACA TCTGATCCCA GCTGCCCTCC AGAACAAGAA GGACATTCTT 
TTTGGGAACC TCCCAGAGAT CTATGAATTC CACAACAGGA TTTTCCTTAA GGAGATCGAA 
AACTGCATTG AAAACCCTGA GCTTCTTGCC CGATGCTTTT TGAAAAGAAA GGAGGACCTC 
CAGATTTATG AGAAATACTG TCAGAACAAG CCAAGGTCTG AAGCTCTCTG GAGACAGTGT 
GGAGACAGCA TCTTTTTCCA GGAATGCCAG CGTAAATTGG ACCATAAGCT CTCCTTAGAC 
GCTTATCTCT TAAAGCCGGT GCAGAGAATC ACCAAGTACC AGCTGCTGCT AAAGGAAATG 
CTGAAGTGCA GCAAGAATTC GGAGGGTACT GCTGAGCTGG AAGAAGCCCT GGCCACGATG 
CTTGATATCA TCAAGTCCGT GAACGACTCC ATGCACCAGA TAGCCATCAC AGGATACGAG 
GGGGACGTTG GTGAGCTTGG CAAGTTGTTG ATGCAAGGTT CCTTCAACGT GTGGACTGAC 
CACAAGAAGG GGCACAACAA AGTGAAGGAC TTGGCTAGGT TCAAACCCAT GCAGAGACAC 
CTCTTCCTCT ACACAAAGAT GCTACTTTTC TGCAAGAAAC GGGAGGAGAA CACAGACGGA 
CATGAGAAGA CAGCTTCATA CAGCTTTAAA AATTCTTTAA AGATGAGCAC TGTGGGCATT 
ACAGAAAATG TAAAAGGGGA TAACAAGAAA TTTGAGATCT GGTATAATGG CAGAGAAGAG 
GTCTATATCA TTCAGGCATC TTCTGTTGAG CTGAAGAACA CCTGGATTTC AGAGATTCGC 
AAAGTCCTGA CAGGACAACT GGAAGCGTGT AGAGAAGCAA GTCAACTGCA CCAAAGGATC 
ACCGAGAGCG TATACCACGC ACCTATGGAC TCCTCCAACC CAAGCAGGTA TAGCAGGAAG 
TCATCGAGTC TGTATGAAAA CAAGTCCGAG ACATCAAACG TGGAAGCATC TAACAACAAA 
AACAGGAATT CCAGTCCCAG CGCCAGACAA AAGAGAGTTG ATGCTTCCAC CAGCCTTAAC 
CTTGAGCGCA CAGCGCTTGC TCGAAAGCGG TTCACATTGC AAGGTCTTTC CAACCGCAGA 
GCTCCACCCA AAGATCCGGA GTGTAAGGAG AGAGAAGTCA CCCGTCGCTT TTCCCTTGCC 
TCCTCCATCA GCTCTGCAGC TAACGCCTCC GCTGGCACCA AAGGTCCCCT TGTTCCTGCA 
GTTAAAGTCA AGAGGCATGA AATAAAGAGT GACCCAACTC CCCTGGGGTT TGAAGATGCT 
TTTGAGAACA CCATTTTGGC TCAGACTAAA TGTAATACCG GTTCCACCAC GAGGCCCGTC 
CCTAGATCTG CTTCACAGAC AGGCTGGCCC GGCAGGCAGA TGCCATCTCT AGACACCTTT 
GAAGATTTTG AAGCCATCCC CTCCAGCACG GACGAGCTCT CCAACTCCTC GGACTTGGAG 
GAAGAGACCA ACCCCAACAA CGGGACCAAT CTCTTTCGGG TGGAAGGCAG CTTTGACAGC 
TGTTTCCCGG ATTCTCTGAC CCTGGAGGAT GGAGATTTAG TGCAGTTTGT GCAGGAAGGA 
GAGAATGGTC AATGGCTGGT GAAGAAGCTG GTCTCTGAGA AGAGCGACTG GGTTCCCTCC 
AGTATCTTGC AGCCCGCAGA GGGGGACAGT CACACCATCA TTAAGAACAG CAATGCAGAC 
ACGTACAACG ATTCCTGCAG CACGGCCTCC CTGGAGTCGG ACACCAAGGA GAATGAGGTG 
GCCAAGAGCT TCCCAGCGGA GCAGAGGGCC GGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795368.1
CDS	364..4182
Translation	MLCSSLCEMLFWLKEEEMSHQELTQRLTTVITHVDEIMQQEIRPLVAVDIIEQLHRQFAILSGGRGKDGAPIITFPEFAGFSDIPDEDFLNVVTYLTSIPSLEAASIGFIIIIDRRRDKWSAVKASLTRIAGAFPGNLQLVFVLRPSRFIQRTIADIGIKLYRDDFKMKVPIIMLNSVSDLHGYIDKSQLTRELGGTLEYGHSQWIHHRTAIENFAMTVKTTAQMLQSFGTDLAETELPNDVQCTEELLSAHTDHHGKLKDELKLAVKQGATLLTCIREPVTRSANSKLSPDELENVATVERLLAQLDETEKAFDQFWTKHHLKLEQCLQLRHFEHDFREVKLALDNLMEGQAGFADIGDSVTRVEHLLREQKHLEEKGQEPLEKAQSLALHGEQLIQNNHYAVDSIRPKCVELRRICDDFTNETKKKYDILGKSLELHKQLDKASQWCEAGIYLLASQAVDKCQSQEGAETALVEIEKFLVTAKEHQLSNPKEFYNQFDMILTPEIKANAQRILQKLEDVQEMFDKRQVSLKKLAAKQTRPVQPVAPHPESSPKRASPKITRPAGVAANRRSTEISCPPKSISEVELSKKKNIKKTKGGIKIEVMHEASQGGSSRVLVMSESDENLSSRRSHIINELIETERVYVEELQSIIEGYASEMDNPSLVHLIPAALQNKKDILFGNLPEIYEFHNRIFLKEIENCIENPELLARCFLKRKEDLQIYEKYCQNKPRSEALWRQCGDSIFFQECQRKLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATMLDIIKSVNDSMHQIAITGYEGDVGELGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYTKMLLFCKKREENTDGHEKTASYSFKNSLKMSTVGITENVKGDNKKFEIWYNGREEVYIIQASSVELKNTWISEIRKVLTGQLEACREASQLHQRITESVYHAPMDSSNPSRYSRKSSSLYENKSETSNVEASNNKNRNSSPSARQKRVDASTSLNLERTALARKRFTLQGLSNRRAPPKDPECKEREVTRRFSLASSISSAANASAGTKGPLVPAVKVKRHEIKSDPTPLGFEDAFENTILAQTKCNTGSTTRPVPRSASQTGWPGRQMPSLDTFEDFEAIPSSTDELSNSSDLEEETNPNNGTNLFRVEGSFDSCFPDSLTLEDGDLVQFVQEGENGQWLVKKLVSEKSDWVPSSILQPAEGDSHTIIKNSNADTYNDSCSTASLESDTKENEVAKSFPAEQRAGS

Target ORF information:

RefSeq Version	XM_014939882.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939882.1

ORF Insert Sequence:

ATGCTGTGCA GTTCCCTGTG TGAAATGCTT TTTTGGTTAA AAGAAGAAGA GATGTCACAT 
CAGGAGCTCA CCCAGCGACT GACTACGGTT ATCACCCATG TGGATGAAAT CATGCAGCAG 
GAGATACGGC CGCTGGTTGC AGTGGATATC ATAGAGCAGC TCCACAGGCA GTTTGCTATC 
TTGTCAGGAG GAAGAGGGAA GGATGGGGCG CCCATTATAA CCTTTCCAGA ATTTGCGGGA 
TTCAGTGACA TACCTGATGA AGATTTTCTG AACGTGGTCA CGTATCTCAC CAGCATCCCC 
AGTCTGGAGG CTGCCAGCAT CGGGTTCATC ATCATCATAG ACAGACGACG GGACAAATGG 
AGCGCAGTCA AGGCATCCCT GACACGGATA GCAGGAGCGT TTCCAGGGAA CCTGCAGCTG 
GTGTTTGTGC TGCGACCCTC CCGCTTCATC CAAAGGACCA TCGCTGACAT TGGCATTAAG 
CTCTATCGAG ATGACTTTAA AATGAAGGTA CCGATCATCA TGTTGAATTC CGTCTCCGAC 
CTGCACGGCT ACATCGACAA AAGCCAGCTG ACGCGGGAGC TGGGGGGAAC CCTGGAGTAT 
GGGCACAGCC AGTGGATCCA CCACCGCACG GCTATTGAAA ACTTTGCAAT GACAGTAAAA 
ACAACAGCAC AGATGCTGCA GAGCTTTGGA ACAGACCTCG CAGAGACTGA ACTTCCCAAC 
GACGTGCAGT GCACGGAGGA GCTGCTCTCC GCACACACAG ACCACCACGG CAAGCTGAAG 
GACGAGCTGA AGCTGGCTGT GAAGCAGGGG GCCACCCTGC TGACCTGCAT CAGGGAGCCG 
GTCACCCGCA GCGCCAACAG CAAGCTCAGC CCTGACGAAC TGGAGAACGT GGCAACAGTA 
GAGAGGTTAC TGGCTCAGTT GGATGAAACG GAAAAGGCTT TTGATCAATT TTGGACCAAG 
CATCACCTAA AACTGGAACA GTGCCTGCAG CTGCGACACT TTGAGCATGA TTTCAGAGAG 
GTAAAACTGG CATTGGATAA TCTCATGGAA GGACAAGCAG GTTTTGCTGA CATTGGAGAC 
AGCGTGACTC GAGTGGAGCA TCTCCTCCGG GAACAGAAAC ACCTGGAAGA GAAAGGACAG 
GAACCTTTGG AGAAGGCCCA GTCTCTAGCT CTCCATGGAG AACAGCTCAT CCAAAACAAT 
CATTATGCAG TTGACTCCAT TAGACCAAAG TGTGTTGAAC TCAGGCGTAT TTGTGATGAC 
TTTACCAATG AGACCAAGAA AAAGTATGAT ATTTTGGGAA AGTCTCTTGA GCTGCACAAG 
CAGTTAGATA AGGCAAGCCA ATGGTGTGAA GCTGGAATCT ACCTCTTGGC TTCCCAAGCT 
GTGGACAAGT GCCAGTCACA AGAGGGAGCT GAAACTGCAC TCGTTGAGAT TGAGAAGTTC 
CTGGTAACAG CCAAGGAGCA CCAGCTCTCC AACCCGAAGG AGTTCTACAA TCAGTTTGAC 
ATGATCCTCA CCCCTGAAAT AAAGGCCAAC GCCCAAAGAA TTCTACAGAA ACTAGAAGAT 
GTACAAGAAA TGTTTGACAA GAGGCAAGTG AGCCTGAAGA AGCTGGCAGC CAAGCAGACG 
CGGCCAGTGC AGCCTGTGGC CCCGCACCCC GAATCTTCCC CAAAGCGAGC CTCACCAAAG 
ATCACCAGAC CAGCAGGAGT AGCTGCCAAC AGGAGATCCA CAGAAATTTC CTGTCCTCCC 
AAGTCCATCT CTGAAGTCGA GTTATCAAAA AAGAAAAACA TTAAGAAGAC TAAAGGTGGA 
ATTAAGATCG AAGTGATGCA CGAGGCCAGC CAGGGAGGGT CCAGCAGGGT GCTGGTGATG 
AGCGAGAGCG ACGAGAACCT CTCCTCCAGG AGGAGCCACA TCATCAATGA GCTGATAGAG 
ACAGAACGGG TTTACGTAGA AGAACTTCAA AGTATAATAG AGGGATACGC CTCGGAAATG 
GACAATCCCA GTTTGGTACA TCTGATCCCA GCTGCCCTCC AGAACAAGAA GGACATTCTT 
TTTGGGAACC TCCCAGAGAT CTATGAATTC CACAACAGGA TTTTCCTTAA GGAGATCGAA 
AACTGCATTG AAAACCCTGA GCTTCTTGCC CGATGCTTTT TGAAAAGAAA GGAGGACCTC 
CAGATTTATG AGAAATACTG TCAGAACAAG CCAAGGTCTG AAGCTCTCTG GAGACAGTGT 
GGAGACAGCA TCTTTTTCCA GGAATGCCAG CGTAAATTGG ACCATAAGCT CTCCTTAGAC 
GCTTATCTCT TAAAGCCGGT GCAGAGAATC ACCAAGTACC AGCTGCTGCT AAAGGAAATG 
CTGAAGTGCA GCAAGAATTC GGAGGGTACT GCTGAGCTGG AAGAAGCCCT GGCCACGATG 
CTTGATATCA TCAAGTCCGT GAACGACTCC ATGCACCAGA TAGCCATCAC AGGATACGAG 
GGGGACGTTG GTGAGCTTGG CAAGTTGTTG ATGCAAGGTT CCTTCAACGT GTGGACTGAC 
CACAAGAAGG GGCACAACAA AGTGAAGGAC TTGGCTAGGT TCAAACCCAT GCAGAGACAC 
CTCTTCCTCT ACACAAAGAT GCTACTTTTC TGCAAGAAAC GGGAGGAGAA CACAGACGGA 
CATGAGAAGA CAGCTTCATA CAGCTTTAAA AATTCTTTAA AGATGAGCAC TGTGGGCATT 
ACAGAAAATG TAAAAGGGGA TAACAAGAAA TTTGAGATCT GGTATAATGG CAGAGAAGAG 
GTCTATATCA TTCAGGCATC TTCTGTTGAG CTGAAGAACA CCTGGATTTC AGAGATTCGC 
AAAGTCCTGA CAGGACAACT GGAAGCGTGT AGAGAAGCAA GTCAACTGCA CCAAAGGATC 
ACCGAGAGCG TATACCACGC ACCTATGGAC TCCTCCAACC CAAGCAGGTA TAGCAGGAAG 
TCATCGAGTC TGTATGAAAA CAAGTCCGAG ACATCAAACG TGGAAGCATC TAACAACAAA 
AACAGGAATT CCAGTCCCAG CGCCAGACAA AAGAGAGTTG ATGCTTCCAC CAGCCTTAAC 
CTTGAGCGCA CAGCGCTTGC TCGAAAGCGG TTCACATTGC AAGGTCTTTC CAACCGCAGA 
GCTCCACCCA AAGATCCGGA GTGTAAGGAG AGAGAAGTCA CCCGTCGCTT TTCCCTTGCC 
TCCTCCATCA GCTCTGCAGC TAACGCCTCC GCTGGCACCA AAGGTCCCCT TGTTCCTGCA 
GTTAAAGTCA AGAGGCATGA AATAAAGAGT GACCCAACTC CCCTGGGGTT TGAAGATGCT 
TTTGAGAACA CCATTTTGGC TCAGACTAAA TGTAATACCG GTTCCACCAC GAGGCCCGTC 
CCTAGATCTG CTTCACAGAC AGGCTGGCCC GGCAGGCAGA TGCCATCTCT AGACACCTTT 
GAAGATTTTG AAGCCATCCC CTCCAGCACG GACGAGCTCT CCAACTCCTC GGACTTGGAG 
GAAGAGACCA ACCCCAACAA CGGGACCAAT CTCTTTCGGG TGGAAGGCAG CTTTGACAGC 
TGTTTCCCGG ATTCTCTGAC CCTGGAGGAT GGAGATTTAG TGCAGTTTGT GCAGGAAGGA 
GAGAATGGTC AATGGCTGGT GAAGAAGCTG GTCTCTGAGA AGAGCGACTG GGTTCCCTCC 
AGTATCTTGC AGCCCGCAGA GGGGGACAGT CACACCATCA TTAAGAACAG CAATGCAGAC 
ACGTACAACG ATTCCTGCAG CACGGCCTCC CTGGAGTCGG ACACCAAGGA GAATGAGGTG 
GCCAAGAGCT TCCCAGCGGA GCAGAGGGCC GGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L2 ( XM_014939883.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795369.1 Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa63498
Clone ID Related Accession (Same CDS sequence)	XM_014939883.1
Accession Version	XM_014939883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable guanine nucleotide exchange factor MCF2L2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGGTAC CGATCATCAT GTTGAATTCC GTCTCCGACC TGCACGGCTA CATCGACAAA 
AGCCAGCTGA CGCGGGAGCT GGGGGGAACC CTGGAGTATG GGCACAGCCA GTGGATCCAC 
CACCGCACGG CTATTGAAAA CTTTGCAATG ACAGTAAAAA CAACAGCACA GATGCTGCAG 
AGCTTTGGAA CAGACCTCGC AGAGACTGAA CTTCCCAACG ACGTGCAGTG CACGGAGGAG 
CTGCTCTCCG CACACACAGA CCACCACGGC AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG 
AAGCAGGGGG CCACCCTGCT GACCTGCATC AGGGAGCCGG TCACCCGCAG CGCCAACAGC 
AAGCTCAGCC CTGACGAACT GGAGAACGTG GCAACAGTAG AGAGGTTACT GGCTCAGTTG 
GATGAAACGG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTGGAACAG 
TGCCTGCAGC TGCGACACTT TGAGCATGAT TTCAGAGAGG TAAAACTGGC ATTGGATAAT 
CTCATGGAAG GACAAGCAGG TTTTGCTGAC ATTGGAGACA GCGTGACTCG AGTGGAGCAT 
CTCCTCCGGG AACAGAAACA CCTGGAAGAG AAAGGACAGG AACCTTTGGA GAAGGCCCAG 
TCTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAATC ATTATGCAGT TGACTCCATT 
AGACCAAAGT GTGTTGAACT CAGGCGTATT TGTGATGACT TTACCAATGA GACCAAGAAA 
AAGTATGATA TTTTGGGAAA GTCTCTTGAG CTGCACAAGC AGTTAGATAA GGCAAGCCAA 
TGGTGTGAAG CTGGAATCTA CCTCTTGGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA 
GAGGGAGCTG AAACTGCACT CGTTGAGATT GAGAAGTTCC TGGTAACAGC CAAGGAGCAC 
CAGCTCTCCA ACCCGAAGGA GTTCTACAAT CAGTTTGACA TGATCCTCAC CCCTGAAATA 
AAGGCCAACG CCCAAAGAAT TCTACAGAAA CTAGAAGATG TACAAGAAAT GTTTGACAAG 
AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC AAGCAGACGC GGCCAGTGCA GCCTGTGGCC 
CCGCACCCCG AATCTTCCCC AAAGCGAGCC TCACCAAAGA TCACCAGACC AGCAGGAGTA 
GCTGCCAACA GGAGATCCAC AGAAATTTCC TGTCCTCCCA AGTCCATCTC TGAAGTCGAG 
TTATCAAAAA AGAAAAACAT TAAGAAGACT AAAGGTGGAA TTAAGATCGA AGTGATGCAC 
GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG CTGGTGATGA GCGAGAGCGA CGAGAACCTC 
TCCTCCAGGA GGAGCCACAT CATCAATGAG CTGATAGAGA CAGAACGGGT TTACGTAGAA 
GAACTTCAAA GTATAATAGA GGGATACGCC TCGGAAATGG ACAATCCCAG TTTGGTACAT 
CTGATCCCAG CTGCCCTCCA GAACAAGAAG GACATTCTTT TTGGGAACCT CCCAGAGATC 
TATGAATTCC ACAACAGGAT TTTCCTTAAG GAGATCGAAA ACTGCATTGA AAACCCTGAG 
CTTCTTGCCC GATGCTTTTT GAAAAGAAAG GAGGACCTCC AGATTTATGA GAAATACTGT 
CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTCCAG 
GAATGCCAGC GTAAATTGGA CCATAAGCTC TCCTTAGACG CTTATCTCTT AAAGCCGGTG 
CAGAGAATCA CCAAGTACCA GCTGCTGCTA AAGGAAATGC TGAAGTGCAG CAAGAATTCG 
GAGGGTACTG CTGAGCTGGA AGAAGCCCTG GCCACGATGC TTGATATCAT CAAGTCCGTG 
AACGACTCCA TGCACCAGAT AGCCATCACA GGATACGAGG GGGACGTTGG TGAGCTTGGC 
AAGTTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG GCACAACAAA 
GTGAAGGACT TGGCTAGGTT CAAACCCATG CAGAGACACC TCTTCCTCTA CACAAAGATG 
CTACTTTTCT GCAAGAAACG GGAGGAGAAC ACAGACGGAC ATGAGAAGAC AGCTTCATAC 
AGCTTTAAAA ATTCTTTAAA GATGAGCACT GTGGGCATTA CAGAAAATGT AAAAGGGGAT 
AACAAGAAAT TTGAGATCTG GTATAATGGC AGAGAAGAGG TCTATATCAT TCAGGCATCT 
TCTGTTGAGC TGAAGAACAC CTGGATTTCA GAGATTCGCA AAGTCCTGAC AGGACAACTG 
GAAGCGTGTA GAGAAGCAAG TCAACTGCAC CAAAGGATCA CCGAGAGCGT ATACCACGCA 
CCTATGGACT CCTCCAACCC AAGCAGGTAT AGCAGGAAGT CATCGAGTCT GTATGAAAAC 
AAGTCCGAGA CATCAAACGT GGAAGCATCT AACAACAAAA ACAGGAATTC CAGTCCCAGC 
GCCAGACAAA AGAGAGATCC GGAGTGTAAG GAGAGAGAAG TCACCCGTCG CTTTTCCCTT 
GCCTCCTCCA TCAGCTCTGC AGCTAACGCC TCCGCTGGCA CCAAAGGTCC CCTTGTTCCT 
GCAGTTAAAG TCAAGAGGCA TGAAATAAAG AGTGACCCAA CTCCCCTGGG GTTTGAAGAT 
GCTTTTGAGA ACACCATTTT GGCTCAGACT AAATGTAATA CCGGTTCCAC CACGAGGCCC 
GTCCCTAGAT CTGCTTCACA GACAGGCTGG CCCGGCAGGC AGATGCCATC TCTAGACACC 
TTTGAAGATT TTGAAGCCAT CCCCTCCAGC ACGGACGAGC TCTCCAACTC CTCGGACTTG 
GAGGAAGAGA CCAACCCCAA CAACGGGACC AATCTCTTTC GGGTGGAAGG CAGCTTTGAC 
AGCTGTTTCC CGGATTCTCT GACCCTGGAG GATGGAGATT TAGTGCAGTT TGTGCAGGAA 
GGAGAGAATG GTCAATGGCT GGTGAAGAAG CTGGTCTCTG AGAAGAGCGA CTGGGTTCCC 
TCCAGTATCT TGCAGCCCGC AGAGGGGGAC AGTCACACCA TCATTAAGAA CAGCAATGCA 
GACACGTACA ACGATTCCTG CAGCACGGCC TCCCTGGAGT CGGACACCAA GGAGAATGAG 
GTGGCCAAGA GCTTCCCAGC GGAGCAGAGG GCCGGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795369.1
CDS	512..4273
Translation	MASRCRKCGQQVPRNPPFTAVRITEGEMQSYHDFIGGCIRLERMAKRDEIMQQEIRPLVAVDIIEQLHRQFAILSGGRGKDGAPIITFPEFAGFSDIPDEDFLNVVTYLTSIPSLEAASIGFIIIIDRRRDKWSAVKASLTRIAGAFPGNLQLVFVLRPSRFIQRTIADIGIKLYRDDFKMKVPIIMLNSVSDLHGYIDKSQLTRELGGTLEYGHSQWIHHRTAIENFAMTVKTTAQMLQSFGTDLAETELPNDVQCTEELLSAHTDHHGKLKDELKLAVKQGATLLTCIREPVTRSANSKLSPDELENVATVERLLAQLDETEKAFDQFWTKHHLKLEQCLQLRHFEHDFREVKLALDNLMEGQAGFADIGDSVTRVEHLLREQKHLEEKGQEPLEKAQSLALHGEQLIQNNHYAVDSIRPKCVELRRICDDFTNETKKKYDILGKSLELHKQLDKASQWCEAGIYLLASQAVDKCQSQEGAETALVEIEKFLVTAKEHQLSNPKEFYNQFDMILTPEIKANAQRILQKLEDVQEMFDKRQVSLKKLAAKQTRPVQPVAPHPESSPKRASPKITRPAGVAANRRSTEISCPPKSISEVELSKKKNIKKTKGGIKIEVMHEASQGGSSRVLVMSESDENLSSRRSHIINELIETERVYVEELQSIIEGYASEMDNPSLVHLIPAALQNKKDILFGNLPEIYEFHNRIFLKEIENCIENPELLARCFLKRKEDLQIYEKYCQNKPRSEALWRQCGDSIFFQECQRKLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATMLDIIKSVNDSMHQIAITGYEGDVGELGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYTKMLLFCKKREENTDGHEKTASYSFKNSLKMSTVGITENVKGDNKKFEIWYNGREEVYIIQASSVELKNTWISEIRKVLTGQLEACREASQLHQRITESVYHAPMDSSNPSRYSRKSSSLYENKSETSNVEASNNKNRNSSPSARQKRDPECKEREVTRRFSLASSISSAANASAGTKGPLVPAVKVKRHEIKSDPTPLGFEDAFENTILAQTKCNTGSTTRPVPRSASQTGWPGRQMPSLDTFEDFEAIPSSTDELSNSSDLEEETNPNNGTNLFRVEGSFDSCFPDSLTLEDGDLVQFVQEGENGQWLVKKLVSEKSDWVPSSILQPAEGDSHTIIKNSNADTYNDSCSTASLESDTKENEVAKSFPAEQRAGS

Target ORF information:

RefSeq Version	XM_014939883.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939883.1

ORF Insert Sequence:

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGGTAC CGATCATCAT GTTGAATTCC GTCTCCGACC TGCACGGCTA CATCGACAAA 
AGCCAGCTGA CGCGGGAGCT GGGGGGAACC CTGGAGTATG GGCACAGCCA GTGGATCCAC 
CACCGCACGG CTATTGAAAA CTTTGCAATG ACAGTAAAAA CAACAGCACA GATGCTGCAG 
AGCTTTGGAA CAGACCTCGC AGAGACTGAA CTTCCCAACG ACGTGCAGTG CACGGAGGAG 
CTGCTCTCCG CACACACAGA CCACCACGGC AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG 
AAGCAGGGGG CCACCCTGCT GACCTGCATC AGGGAGCCGG TCACCCGCAG CGCCAACAGC 
AAGCTCAGCC CTGACGAACT GGAGAACGTG GCAACAGTAG AGAGGTTACT GGCTCAGTTG 
GATGAAACGG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTGGAACAG 
TGCCTGCAGC TGCGACACTT TGAGCATGAT TTCAGAGAGG TAAAACTGGC ATTGGATAAT 
CTCATGGAAG GACAAGCAGG TTTTGCTGAC ATTGGAGACA GCGTGACTCG AGTGGAGCAT 
CTCCTCCGGG AACAGAAACA CCTGGAAGAG AAAGGACAGG AACCTTTGGA GAAGGCCCAG 
TCTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAATC ATTATGCAGT TGACTCCATT 
AGACCAAAGT GTGTTGAACT CAGGCGTATT TGTGATGACT TTACCAATGA GACCAAGAAA 
AAGTATGATA TTTTGGGAAA GTCTCTTGAG CTGCACAAGC AGTTAGATAA GGCAAGCCAA 
TGGTGTGAAG CTGGAATCTA CCTCTTGGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA 
GAGGGAGCTG AAACTGCACT CGTTGAGATT GAGAAGTTCC TGGTAACAGC CAAGGAGCAC 
CAGCTCTCCA ACCCGAAGGA GTTCTACAAT CAGTTTGACA TGATCCTCAC CCCTGAAATA 
AAGGCCAACG CCCAAAGAAT TCTACAGAAA CTAGAAGATG TACAAGAAAT GTTTGACAAG 
AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC AAGCAGACGC GGCCAGTGCA GCCTGTGGCC 
CCGCACCCCG AATCTTCCCC AAAGCGAGCC TCACCAAAGA TCACCAGACC AGCAGGAGTA 
GCTGCCAACA GGAGATCCAC AGAAATTTCC TGTCCTCCCA AGTCCATCTC TGAAGTCGAG 
TTATCAAAAA AGAAAAACAT TAAGAAGACT AAAGGTGGAA TTAAGATCGA AGTGATGCAC 
GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG CTGGTGATGA GCGAGAGCGA CGAGAACCTC 
TCCTCCAGGA GGAGCCACAT CATCAATGAG CTGATAGAGA CAGAACGGGT TTACGTAGAA 
GAACTTCAAA GTATAATAGA GGGATACGCC TCGGAAATGG ACAATCCCAG TTTGGTACAT 
CTGATCCCAG CTGCCCTCCA GAACAAGAAG GACATTCTTT TTGGGAACCT CCCAGAGATC 
TATGAATTCC ACAACAGGAT TTTCCTTAAG GAGATCGAAA ACTGCATTGA AAACCCTGAG 
CTTCTTGCCC GATGCTTTTT GAAAAGAAAG GAGGACCTCC AGATTTATGA GAAATACTGT 
CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTCCAG 
GAATGCCAGC GTAAATTGGA CCATAAGCTC TCCTTAGACG CTTATCTCTT AAAGCCGGTG 
CAGAGAATCA CCAAGTACCA GCTGCTGCTA AAGGAAATGC TGAAGTGCAG CAAGAATTCG 
GAGGGTACTG CTGAGCTGGA AGAAGCCCTG GCCACGATGC TTGATATCAT CAAGTCCGTG 
AACGACTCCA TGCACCAGAT AGCCATCACA GGATACGAGG GGGACGTTGG TGAGCTTGGC 
AAGTTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG GCACAACAAA 
GTGAAGGACT TGGCTAGGTT CAAACCCATG CAGAGACACC TCTTCCTCTA CACAAAGATG 
CTACTTTTCT GCAAGAAACG GGAGGAGAAC ACAGACGGAC ATGAGAAGAC AGCTTCATAC 
AGCTTTAAAA ATTCTTTAAA GATGAGCACT GTGGGCATTA CAGAAAATGT AAAAGGGGAT 
AACAAGAAAT TTGAGATCTG GTATAATGGC AGAGAAGAGG TCTATATCAT TCAGGCATCT 
TCTGTTGAGC TGAAGAACAC CTGGATTTCA GAGATTCGCA AAGTCCTGAC AGGACAACTG 
GAAGCGTGTA GAGAAGCAAG TCAACTGCAC CAAAGGATCA CCGAGAGCGT ATACCACGCA 
CCTATGGACT CCTCCAACCC AAGCAGGTAT AGCAGGAAGT CATCGAGTCT GTATGAAAAC 
AAGTCCGAGA CATCAAACGT GGAAGCATCT AACAACAAAA ACAGGAATTC CAGTCCCAGC 
GCCAGACAAA AGAGAGATCC GGAGTGTAAG GAGAGAGAAG TCACCCGTCG CTTTTCCCTT 
GCCTCCTCCA TCAGCTCTGC AGCTAACGCC TCCGCTGGCA CCAAAGGTCC CCTTGTTCCT 
GCAGTTAAAG TCAAGAGGCA TGAAATAAAG AGTGACCCAA CTCCCCTGGG GTTTGAAGAT 
GCTTTTGAGA ACACCATTTT GGCTCAGACT AAATGTAATA CCGGTTCCAC CACGAGGCCC 
GTCCCTAGAT CTGCTTCACA GACAGGCTGG CCCGGCAGGC AGATGCCATC TCTAGACACC 
TTTGAAGATT TTGAAGCCAT CCCCTCCAGC ACGGACGAGC TCTCCAACTC CTCGGACTTG 
GAGGAAGAGA CCAACCCCAA CAACGGGACC AATCTCTTTC GGGTGGAAGG CAGCTTTGAC 
AGCTGTTTCC CGGATTCTCT GACCCTGGAG GATGGAGATT TAGTGCAGTT TGTGCAGGAA 
GGAGAGAATG GTCAATGGCT GGTGAAGAAG CTGGTCTCTG AGAAGAGCGA CTGGGTTCCC 
TCCAGTATCT TGCAGCCCGC AGAGGGGGAC AGTCACACCA TCATTAAGAA CAGCAATGCA 
GACACGTACA ACGATTCCTG CAGCACGGCC TCCCTGGAGT CGGACACCAA GGAGAATGAG 
GTGGCCAAGA GCTTCCCAGC GGAGCAGAGG GCCGGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L2 ( XM_014939884.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795370.1 Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCa63499
Clone ID Related Accession (Same CDS sequence)	XM_014939884.1
Accession Version	XM_014939884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable guanine nucleotide exchange factor MCF2L2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGG AGATACGGCC GCTGGTTGCA GTGGATATCA TAGAGCAGCT CCACAGGCAG 
TTTGCTATCT TGTCAGGAGG AAGAGGGAAG GATGGGGCGC CCATTATAAC CTTTCCAGAA 
TTTGCGGGAT TCAGTGACAT ACCTGATGAA GATTTTCTGA ACGTGGTCAC GTATCTCACC 
AGCATCCCCA GTCTGGAGGC TGCCAGCATC GGGTTCATCA TCATCATAGA CAGACGACGG 
GACAAATGGA GCGCAGTCAA GGCATCCCTG ACACGGATAG CAGGAGCGTT TCCAGGGAAC 
CTGCAGCTGG TGTTTGTGCT GCGACCCTCC CGCTTCATCC AAAGGACCAT CGCTGACATT 
GGCATTAAGC TCTATCGAGA TGACTTTAAA ATGAAGGTAC CGATCATCAT GTTGAATTCC 
GTCTCCGACC TGCACGGCTA CATCGACAAA AGCCAGCTGA CGCGGGAGCT GGGGGGAACC 
CTGGAGTATG GGCACAGCCA GTGGATCCAC CACCGCACGG CTATTGAAAA CTTTGCAATG 
ACAGTAAAAA CAACAGCACA GATGCTGCAG AGCTTTGGAA CAGACCTCGC AGAGACTGAA 
CTTCCCAACG ACGTGCAGTG CACGGAGGAG CTGCTCTCCG CACACACAGA CCACCACGGC 
AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG AAGCAGGGGG CCACCCTGCT GACCTGCATC 
AGGGAGCCGG TCACCCGCAG CGCCAACAGC AAGCTCAGCC CTGACGAACT GGAGAACGTG 
GCAACAGTAG AGAGGTTACT GGCTCAGTTG GATGAAACGG AAAAGGCTTT TGATCAATTT 
TGGACCAAGC ATCACCTAAA ACTGGAACAG TGCCTGCAGC TGCGACACTT TGAGCATGAT 
TTCAGAGAGG TAAAACTGGC ATTGGATAAT CTCATGGAAG GACAAGCAGG TTTTGCTGAC 
ATTGGAGACA GCGTGACTCG AGTGGAGCAT CTCCTCCGGG AACAGAAACA CCTGGAAGAG 
AAAGGACAGG AACCTTTGGA GAAGGCCCAG TCTCTAGCTC TCCATGGAGA ACAGCTCATC 
CAAAACAATC ATTATGCAGT TGACTCCATT AGACCAAAGT GTGTTGAACT CAGGCGTATT 
TGTGATGACT TTACCAATGA GACCAAGAAA AAGTATGATA TTTTGGGAAA GTCTCTTGAG 
CTGCACAAGC AGTTAGATAA GGCAAGCCAA TGGTGTGAAG CTGGAATCTA CCTCTTGGCT 
TCCCAAGCTG TGGACAAGTG CCAGTCACAA GAGGGAGCTG AAACTGCACT CGTTGAGATT 
GAGAAGTTCC TGGTAACAGC CAAGGAGCAC CAGCTCTCCA ACCCGAAGGA GTTCTACAAT 
CAGTTTGACA TGATCCTCAC CCCTGAAATA AAGGCCAACG CCCAAAGAAT TCTACAGAAA 
CTAGAAGATG TACAAGAAAT GTTTGACAAG AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC 
AAGCAGACGC GGCCAGTGCA GCCTGTGGCC CCGCACCCCG AATCTTCCCC AAAGCGAGCC 
TCACCAAAGA TCACCAGACC AGCAGGAGTA GCTGCCAACA GGAGATCCAC AGAAATTTCC 
TGTCCTCCCA AGTCCATCTC TGAAGTCGAG TTATCAAAAA AGAAAAACAT TAAGAAGACT 
AAAGGTGGAA TTAAGATCGA AGTGATGCAC GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG 
CTGGTGATGA GCGAGAGCGA CGAGAACCTC TCCTCCAGGA GGAGCCACAT CATCAATGAG 
CTGATAGAGA CAGAACGGGT TTACGTAGAA GAACTTCAAA GTATAATAGA GGGATACGCC 
TCGGAAATGG ACAATCCCAG TTTGGTACAT CTGATCCCAG CTGCCCTCCA GAACAAGAAG 
GACATTCTTT TTGGGAACCT CCCAGAGATC TATGAATTCC ACAACAGGAT TTTCCTTAAG 
GAGATCGAAA ACTGCATTGA AAACCCTGAG CTTCTTGCCC GATGCTTTTT GAAAAGAAAG 
GAGGACCTCC AGATTTATGA GAAATACTGT CAGAACAAGC CAAGGTCTGA AGCTCTCTGG 
AGACAGTGTG GAGACAGCAT CTTTTTCCAG GAATGCCAGC GTAAATTGGA CCATAAGCTC 
TCCTTAGACG CTTATCTCTT AAAGCCGGTG CAGAGAATCA CCAAGTACCA GCTGCTGCTA 
AAGGAAATGC TGAAGTGCAG CAAGAATTCG GAGGGTACTG CTGAGCTGGA AGAAGCCCTG 
GCCACGATGC TTGATATCAT CAAGTCCGTG AACGACTCCA TGCACCAGAT AGCCATCACA 
GGATACGAGG GGGACGTTGG TGAGCTTGGC AAGTTGTTGA TGCAAGGTTC CTTCAACGTG 
TGGACTGACC ACAAGAAGGG GCACAACAAA GTGAAGGACT TGGCTAGGTT CAAACCCATG 
CAGAGACACC TCTTCCTCTA CACAAAGATG CTACTTTTCT GCAAGAAACG GGAGGAGAAC 
ACAGACGGAC ATGAGAAGAC AGCTTCATAC AGCTTTAAAA ATTCTTTAAA GATGAGCACT 
GTGGGCATTA CAGAAAATGT AAAAGGGGAT AACAAGAAAT TTGAGATCTG GTATAATGGC 
AGAGAAGAGG TCTATATCAT TCAGGCATCT TCTGTTGAGC TGAAGAACAC CTGGATTTCA 
GAGATTCGCA AAGTCCTGAC AGGACAACTG GAAGCGTGTA GAGAAGCAAG TCAACTGCAC 
CAAAGGATCA CCGAGAGCGT ATACCACGCA CCTATGGACT CCTCCAACCC AAGCAGGTAT 
AGCAGGAAGT CATCGAGTCT GTATGAAAAC AAGTCCGAGA CATCAAACGT GGAAGCATCT 
AACAACAAAA ACAGGAATTC CAGTCCCAGC GCCAGACAAA AGAGAGTTGA TGCTTCCACC 
AGCCTTAACC TTGAGCGCAC AGCGCTTGCT CGAAAGCGGT TCACATTGCA AGGTCTTTCC 
AACCGCAGAG CTCCACCCAA AGATCCGGAG TGTAAGGAGA GAGAAGTCAC CCGTCGCTTT 
TCCCTTGCCT CCTCCATCAG CTCTGCAGCT AACGCCTCCG CTGGCACCAA AGGTCCCCTT 
GTTCCTGCAG TTAAAGTCAA GAGGCATGAA ATAAAGAGTG ACCCAACTCC CCTGGGGTTT 
GAAGATGCTT TTGAGAACAC CATTTTGGCT CAGACTAAAT GTAATACCGG TTCCACCACG 
AGGCCCGTCC CTAGATCTGC TTCACAGACA GGCTGGCCCG GCAGGCAGAT GCCATCTCTA 
GACACCTTTG AAGATTTTGA AGCCATCCCC TCCAGCACGG ACGAGCTCTC CAACTCCTCG 
GACTTGGAGG AAGAGACCAA CCCCAACAAC GGGACCAATC TCTTTCGGGT GGAAGGCAGC 
TTTGACAGCT GTTTCCCGGA TTCTCTGACC CTGGAGGATG GAGATTTAGT GCAGTTTGTG 
CAGGAAGGAG AGAATGGTCA ATGGCTGGTG AAGAAGCTGG TCTCTGAGAA GAGCGACTGG 
GTTCCCTCCA GTATCTTGCA GCCCGCAGAG GGGGACAGTC ACACCATCAT TAAGAACAGC 
AATGCAGACA CGTACAACGA TTCCTGCAGC ACGGCCTCCC TGGAGTCGGA CACCAAGGAG 
AATGAGGTGG CCAAGAGCTT CCCAGCGGAG CAGAGGGCCG GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795370.1
CDS	126..3833
Translation	MQQEIRPLVAVDIIEQLHRQFAILSGGRGKDGAPIITFPEFAGFSDIPDEDFLNVVTYLTSIPSLEAASIGFIIIIDRRRDKWSAVKASLTRIAGAFPGNLQLVFVLRPSRFIQRTIADIGIKLYRDDFKMKVPIIMLNSVSDLHGYIDKSQLTRELGGTLEYGHSQWIHHRTAIENFAMTVKTTAQMLQSFGTDLAETELPNDVQCTEELLSAHTDHHGKLKDELKLAVKQGATLLTCIREPVTRSANSKLSPDELENVATVERLLAQLDETEKAFDQFWTKHHLKLEQCLQLRHFEHDFREVKLALDNLMEGQAGFADIGDSVTRVEHLLREQKHLEEKGQEPLEKAQSLALHGEQLIQNNHYAVDSIRPKCVELRRICDDFTNETKKKYDILGKSLELHKQLDKASQWCEAGIYLLASQAVDKCQSQEGAETALVEIEKFLVTAKEHQLSNPKEFYNQFDMILTPEIKANAQRILQKLEDVQEMFDKRQVSLKKLAAKQTRPVQPVAPHPESSPKRASPKITRPAGVAANRRSTEISCPPKSISEVELSKKKNIKKTKGGIKIEVMHEASQGGSSRVLVMSESDENLSSRRSHIINELIETERVYVEELQSIIEGYASEMDNPSLVHLIPAALQNKKDILFGNLPEIYEFHNRIFLKEIENCIENPELLARCFLKRKEDLQIYEKYCQNKPRSEALWRQCGDSIFFQECQRKLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATMLDIIKSVNDSMHQIAITGYEGDVGELGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYTKMLLFCKKREENTDGHEKTASYSFKNSLKMSTVGITENVKGDNKKFEIWYNGREEVYIIQASSVELKNTWISEIRKVLTGQLEACREASQLHQRITESVYHAPMDSSNPSRYSRKSSSLYENKSETSNVEASNNKNRNSSPSARQKRVDASTSLNLERTALARKRFTLQGLSNRRAPPKDPECKEREVTRRFSLASSISSAANASAGTKGPLVPAVKVKRHEIKSDPTPLGFEDAFENTILAQTKCNTGSTTRPVPRSASQTGWPGRQMPSLDTFEDFEAIPSSTDELSNSSDLEEETNPNNGTNLFRVEGSFDSCFPDSLTLEDGDLVQFVQEGENGQWLVKKLVSEKSDWVPSSILQPAEGDSHTIIKNSNADTYNDSCSTASLESDTKENEVAKSFPAEQRAGS

Target ORF information:

RefSeq Version	XM_014939884.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939884.1

ORF Insert Sequence:

ATGCAGCAGG AGATACGGCC GCTGGTTGCA GTGGATATCA TAGAGCAGCT CCACAGGCAG 
TTTGCTATCT TGTCAGGAGG AAGAGGGAAG GATGGGGCGC CCATTATAAC CTTTCCAGAA 
TTTGCGGGAT TCAGTGACAT ACCTGATGAA GATTTTCTGA ACGTGGTCAC GTATCTCACC 
AGCATCCCCA GTCTGGAGGC TGCCAGCATC GGGTTCATCA TCATCATAGA CAGACGACGG 
GACAAATGGA GCGCAGTCAA GGCATCCCTG ACACGGATAG CAGGAGCGTT TCCAGGGAAC 
CTGCAGCTGG TGTTTGTGCT GCGACCCTCC CGCTTCATCC AAAGGACCAT CGCTGACATT 
GGCATTAAGC TCTATCGAGA TGACTTTAAA ATGAAGGTAC CGATCATCAT GTTGAATTCC 
GTCTCCGACC TGCACGGCTA CATCGACAAA AGCCAGCTGA CGCGGGAGCT GGGGGGAACC 
CTGGAGTATG GGCACAGCCA GTGGATCCAC CACCGCACGG CTATTGAAAA CTTTGCAATG 
ACAGTAAAAA CAACAGCACA GATGCTGCAG AGCTTTGGAA CAGACCTCGC AGAGACTGAA 
CTTCCCAACG ACGTGCAGTG CACGGAGGAG CTGCTCTCCG CACACACAGA CCACCACGGC 
AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG AAGCAGGGGG CCACCCTGCT GACCTGCATC 
AGGGAGCCGG TCACCCGCAG CGCCAACAGC AAGCTCAGCC CTGACGAACT GGAGAACGTG 
GCAACAGTAG AGAGGTTACT GGCTCAGTTG GATGAAACGG AAAAGGCTTT TGATCAATTT 
TGGACCAAGC ATCACCTAAA ACTGGAACAG TGCCTGCAGC TGCGACACTT TGAGCATGAT 
TTCAGAGAGG TAAAACTGGC ATTGGATAAT CTCATGGAAG GACAAGCAGG TTTTGCTGAC 
ATTGGAGACA GCGTGACTCG AGTGGAGCAT CTCCTCCGGG AACAGAAACA CCTGGAAGAG 
AAAGGACAGG AACCTTTGGA GAAGGCCCAG TCTCTAGCTC TCCATGGAGA ACAGCTCATC 
CAAAACAATC ATTATGCAGT TGACTCCATT AGACCAAAGT GTGTTGAACT CAGGCGTATT 
TGTGATGACT TTACCAATGA GACCAAGAAA AAGTATGATA TTTTGGGAAA GTCTCTTGAG 
CTGCACAAGC AGTTAGATAA GGCAAGCCAA TGGTGTGAAG CTGGAATCTA CCTCTTGGCT 
TCCCAAGCTG TGGACAAGTG CCAGTCACAA GAGGGAGCTG AAACTGCACT CGTTGAGATT 
GAGAAGTTCC TGGTAACAGC CAAGGAGCAC CAGCTCTCCA ACCCGAAGGA GTTCTACAAT 
CAGTTTGACA TGATCCTCAC CCCTGAAATA AAGGCCAACG CCCAAAGAAT TCTACAGAAA 
CTAGAAGATG TACAAGAAAT GTTTGACAAG AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC 
AAGCAGACGC GGCCAGTGCA GCCTGTGGCC CCGCACCCCG AATCTTCCCC AAAGCGAGCC 
TCACCAAAGA TCACCAGACC AGCAGGAGTA GCTGCCAACA GGAGATCCAC AGAAATTTCC 
TGTCCTCCCA AGTCCATCTC TGAAGTCGAG TTATCAAAAA AGAAAAACAT TAAGAAGACT 
AAAGGTGGAA TTAAGATCGA AGTGATGCAC GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG 
CTGGTGATGA GCGAGAGCGA CGAGAACCTC TCCTCCAGGA GGAGCCACAT CATCAATGAG 
CTGATAGAGA CAGAACGGGT TTACGTAGAA GAACTTCAAA GTATAATAGA GGGATACGCC 
TCGGAAATGG ACAATCCCAG TTTGGTACAT CTGATCCCAG CTGCCCTCCA GAACAAGAAG 
GACATTCTTT TTGGGAACCT CCCAGAGATC TATGAATTCC ACAACAGGAT TTTCCTTAAG 
GAGATCGAAA ACTGCATTGA AAACCCTGAG CTTCTTGCCC GATGCTTTTT GAAAAGAAAG 
GAGGACCTCC AGATTTATGA GAAATACTGT CAGAACAAGC CAAGGTCTGA AGCTCTCTGG 
AGACAGTGTG GAGACAGCAT CTTTTTCCAG GAATGCCAGC GTAAATTGGA CCATAAGCTC 
TCCTTAGACG CTTATCTCTT AAAGCCGGTG CAGAGAATCA CCAAGTACCA GCTGCTGCTA 
AAGGAAATGC TGAAGTGCAG CAAGAATTCG GAGGGTACTG CTGAGCTGGA AGAAGCCCTG 
GCCACGATGC TTGATATCAT CAAGTCCGTG AACGACTCCA TGCACCAGAT AGCCATCACA 
GGATACGAGG GGGACGTTGG TGAGCTTGGC AAGTTGTTGA TGCAAGGTTC CTTCAACGTG 
TGGACTGACC ACAAGAAGGG GCACAACAAA GTGAAGGACT TGGCTAGGTT CAAACCCATG 
CAGAGACACC TCTTCCTCTA CACAAAGATG CTACTTTTCT GCAAGAAACG GGAGGAGAAC 
ACAGACGGAC ATGAGAAGAC AGCTTCATAC AGCTTTAAAA ATTCTTTAAA GATGAGCACT 
GTGGGCATTA CAGAAAATGT AAAAGGGGAT AACAAGAAAT TTGAGATCTG GTATAATGGC 
AGAGAAGAGG TCTATATCAT TCAGGCATCT TCTGTTGAGC TGAAGAACAC CTGGATTTCA 
GAGATTCGCA AAGTCCTGAC AGGACAACTG GAAGCGTGTA GAGAAGCAAG TCAACTGCAC 
CAAAGGATCA CCGAGAGCGT ATACCACGCA CCTATGGACT CCTCCAACCC AAGCAGGTAT 
AGCAGGAAGT CATCGAGTCT GTATGAAAAC AAGTCCGAGA CATCAAACGT GGAAGCATCT 
AACAACAAAA ACAGGAATTC CAGTCCCAGC GCCAGACAAA AGAGAGTTGA TGCTTCCACC 
AGCCTTAACC TTGAGCGCAC AGCGCTTGCT CGAAAGCGGT TCACATTGCA AGGTCTTTCC 
AACCGCAGAG CTCCACCCAA AGATCCGGAG TGTAAGGAGA GAGAAGTCAC CCGTCGCTTT 
TCCCTTGCCT CCTCCATCAG CTCTGCAGCT AACGCCTCCG CTGGCACCAA AGGTCCCCTT 
GTTCCTGCAG TTAAAGTCAA GAGGCATGAA ATAAAGAGTG ACCCAACTCC CCTGGGGTTT 
GAAGATGCTT TTGAGAACAC CATTTTGGCT CAGACTAAAT GTAATACCGG TTCCACCACG 
AGGCCCGTCC CTAGATCTGC TTCACAGACA GGCTGGCCCG GCAGGCAGAT GCCATCTCTA 
GACACCTTTG AAGATTTTGA AGCCATCCCC TCCAGCACGG ACGAGCTCTC CAACTCCTCG 
GACTTGGAGG AAGAGACCAA CCCCAACAAC GGGACCAATC TCTTTCGGGT GGAAGGCAGC 
TTTGACAGCT GTTTCCCGGA TTCTCTGACC CTGGAGGATG GAGATTTAGT GCAGTTTGTG 
CAGGAAGGAG AGAATGGTCA ATGGCTGGTG AAGAAGCTGG TCTCTGAGAA GAGCGACTGG 
GTTCCCTCCA GTATCTTGCA GCCCGCAGAG GGGGACAGTC ACACCATCAT TAAGAACAGC 
AATGCAGACA CGTACAACGA TTCCTGCAGC ACGGCCTCCC TGGAGTCGGA CACCAAGGAG 
AATGAGGTGG CCAAGAGCTT CCCAGCGGAG CAGAGGGCCG GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L2 ( XM_014939885.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795371.1 Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X6, mRNA.

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CloneID	OCa63500
Clone ID Related Accession (Same CDS sequence)	XM_014939885.1
Accession Version	XM_014939885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable guanine nucleotide exchange factor MCF2L2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGGTAC CGATCATCAT GTTGAATTCC GTCTCCGACC TGCACGGCTA CATCGACAAA 
AGCCAGCTGA CGCGGGAGCT GGGGGGAACC CTGGAGTATG GGCACAGCCA GTGGATCCAC 
CACCGCACGG CTATTGAAAA CTTTGCAATG ACAGTAAAAA CAACAGCACA GATGCTGCAG 
AGCTTTGGAA CAGACCTCGC AGAGACTGAA CTTCCCAACG ACGTGCAGTG CACGGAGGAG 
CTGCTCTCCG CACACACAGA CCACCACGGC AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG 
AAGCAGGGGG CCACCCTGCT GACCTGCATC AGGGAGCCGG TCACCCGCAG CGCCAACAGC 
AAGCTCAGCC CTGACGAACT GGAGAACGTG GCAACAGTAG AGAGGTTACT GGCTCAGTTG 
GATGAAACGG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTGGAACAG 
TGCCTGCAGC TGCGACACTT TGAGCATGAT TTCAGAGAGG TAAAACTGGC ATTGGATAAT 
CTCATGGAAG GACAAGCAGG TTTTGCTGAC ATTGGAGACA GCGTGACTCG AGTGGAGCAT 
CTCCTCCGGG AACAGAAACA CCTGGAAGAG AAAGGACAGG AACCTTTGGA GAAGGCCCAG 
TCTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAATC ATTATGCAGT TGACTCCATT 
AGACCAAAGT GTGTTGAACT CAGGCGTATT TGTGATGACT TTACCAATGA GACCAAGAAA 
AAGTATGATA TTTTGGGAAA GTCTCTTGAG CTGCACAAGC AGTTAGATAA GGCAAGCCAA 
TGGTGTGAAG CTGGAATCTA CCTCTTGGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA 
GAGGGAGCTG AAACTGCACT CGTTGAGATT GAGAAGTTCC TGGTAACAGC CAAGGAGCAC 
CAGCTCTCCA ACCCGAAGGA GTTCTACAAT CAGTTTGACA TGATCCTCAC CCCTGAAATA 
AAGGCCAACG CCCAAAGAAT TCTACAGAAA CTAGAAGATG TACAAGAAAT GTTTGACAAG 
AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC AAGCAGACGC GGCCAGTGCA GCCTGTGGCC 
CCGCACCCCG AATCTTCCCC AAAGCGAGCC TCACCAAAGA TCACCAGACC AGCAGGAGTA 
GCTGCCAACA GGAGATCCAC AGAAATTTCC TGTCCTCCCA AGTCCATCTC TGAAGTCGAG 
TTATCAAAAA AGAAAAACAT TAAGAAGACT AAAGGTGGAA TTAAGATCGA AGTGATGCAC 
GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG CTGGTGATGA GCGAGAGCGA CGAGAACCTC 
TCCTCCAGGA GGAGCCACAT CATCAATGAG CTGATAGAGA CAGAACGGGT TTACGTAGAA 
GAACTTCAAA GTATAATAGA GGGATACGCC TCGGAAATGG ACAATCCCAG TTTGGTACAT 
CTGATCCCAG CTGCCCTCCA GAACAAGAAG GACATTCTTT TTGGGAACCT CCCAGAGATC 
TATGAATTCC ACAACAGGAT TTTCCTTAAG GAGATCGAAA ACTGCATTGA AAACCCTGAG 
CTTCTTGCCC GATGCTTTTT GAAAAGAAAG GAGGACCTCC AGATTTATGA GAAATACTGT 
CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTCCAG 
GAATGCCAGC GTAAATTGGA CCATAAGCTC TCCTTAGACG CTTATCTCTT AAAGCCGGTG 
CAGAGAATCA CCAAGTACCA GCTGCTGCTA AAGGAAATGC TGAAGTGCAG CAAGAATTCG 
GAGGGTACTG CTGAGCTGGA AGAAGCCCTG GCCACGATGC TTGATATCAT CAAGTCCGTG 
AACGACTCCA TGCACCAGAT AGCCATCACA GGATACGAGG GGGACGTTGG TGAGCTTGGC 
AAGTTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG GCACAACAAA 
GTGAAGGACT TGGCTAGGTT CAAACCCATG CAGAGACACC TCTTCCTCTA CACAAAGATG 
CTACTTTTCT GCAAGAAACG GGAGGAGAAC ACAGACGGAC ATGAGAAGAC AGCTTCATAC 
AGCTTTAAAA ATTCTTTAAA GATGAGCACT GTGGGCATTA CAGAAAATGT AAAAGGGGAT 
AACAAGAAAT TTGAGATCTG GTATAATGGC AGAGAAGAGG TCTATATCAT TCAGGCATCT 
TCTGTTGAGC TGAAGAACAC CTGGATTTCA GAGATTCGCA AAGTCCTGAC AGGACAACTG 
GAAGCGTGTA GAGAAGCAAG TCAACTGCAC CAAAGGATCA CCGAGAGCGT ATACCACGCA 
CCTATGGACT CCTCCAACCC AAGCAGGTAT AGCAGGAAGT CATCGAGTCT GTATGAAAAC 
AAGTCCGAGA CATCAAACGT GGAAGCATCT AACAACAAAA ACAGGAATTC CAGTCCCAGC 
GCCAGACAAA AGAGAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGGCA TGAAATAAAG 
AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATTTT GGCTCAGACT 
AAATGTAATA CCGGTTCCAC CACGAGGCCC GTCCCTAGAT CTGCTTCACA GACAGGCTGG 
CCCGGCAGGC AGATGCCATC TCTAGACACC TTTGAAGATT TTGAAGCCAT CCCCTCCAGC 
ACGGACGAGC TCTCCAACTC CTCGGACTTG GAGGAAGAGA CCAACCCCAA CAACGGGACC 
AATCTCTTTC GGGTGGAAGG CAGCTTTGAC AGCTGTTTCC CGGATTCTCT GACCCTGGAG 
GATGGAGATT TAGTGCAGTT TGTGCAGGAA GGAGAGAATG GTCAATGGCT GGTGAAGAAG 
CTGGTCTCTG AGAAGAGCGA CTGGGTTCCC TCCAGTATCT TGCAGCCCGC AGAGGGGGAC 
AGTCACACCA TCATTAAGAA CAGCAATGCA GACACGTACA ACGATTCCTG CAGCACGGCC 
TCCCTGGAGT CGGACACCAA GGAGAATGAG GTGGCCAAGA GCTTCCCAGC GGAGCAGAGG 
GCCGGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795371.1
CDS	512..4183
Translation	MASRCRKCGQQVPRNPPFTAVRITEGEMQSYHDFIGGCIRLERMAKRDEIMQQEIRPLVAVDIIEQLHRQFAILSGGRGKDGAPIITFPEFAGFSDIPDEDFLNVVTYLTSIPSLEAASIGFIIIIDRRRDKWSAVKASLTRIAGAFPGNLQLVFVLRPSRFIQRTIADIGIKLYRDDFKMKVPIIMLNSVSDLHGYIDKSQLTRELGGTLEYGHSQWIHHRTAIENFAMTVKTTAQMLQSFGTDLAETELPNDVQCTEELLSAHTDHHGKLKDELKLAVKQGATLLTCIREPVTRSANSKLSPDELENVATVERLLAQLDETEKAFDQFWTKHHLKLEQCLQLRHFEHDFREVKLALDNLMEGQAGFADIGDSVTRVEHLLREQKHLEEKGQEPLEKAQSLALHGEQLIQNNHYAVDSIRPKCVELRRICDDFTNETKKKYDILGKSLELHKQLDKASQWCEAGIYLLASQAVDKCQSQEGAETALVEIEKFLVTAKEHQLSNPKEFYNQFDMILTPEIKANAQRILQKLEDVQEMFDKRQVSLKKLAAKQTRPVQPVAPHPESSPKRASPKITRPAGVAANRRSTEISCPPKSISEVELSKKKNIKKTKGGIKIEVMHEASQGGSSRVLVMSESDENLSSRRSHIINELIETERVYVEELQSIIEGYASEMDNPSLVHLIPAALQNKKDILFGNLPEIYEFHNRIFLKEIENCIENPELLARCFLKRKEDLQIYEKYCQNKPRSEALWRQCGDSIFFQECQRKLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATMLDIIKSVNDSMHQIAITGYEGDVGELGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYTKMLLFCKKREENTDGHEKTASYSFKNSLKMSTVGITENVKGDNKKFEIWYNGREEVYIIQASSVELKNTWISEIRKVLTGQLEACREASQLHQRITESVYHAPMDSSNPSRYSRKSSSLYENKSETSNVEASNNKNRNSSPSARQKRGPLVPAVKVKRHEIKSDPTPLGFEDAFENTILAQTKCNTGSTTRPVPRSASQTGWPGRQMPSLDTFEDFEAIPSSTDELSNSSDLEEETNPNNGTNLFRVEGSFDSCFPDSLTLEDGDLVQFVQEGENGQWLVKKLVSEKSDWVPSSILQPAEGDSHTIIKNSNADTYNDSCSTASLESDTKENEVAKSFPAEQRAGS

Target ORF information:

RefSeq Version	XM_014939885.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939885.1

ORF Insert Sequence:

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGGTAC CGATCATCAT GTTGAATTCC GTCTCCGACC TGCACGGCTA CATCGACAAA 
AGCCAGCTGA CGCGGGAGCT GGGGGGAACC CTGGAGTATG GGCACAGCCA GTGGATCCAC 
CACCGCACGG CTATTGAAAA CTTTGCAATG ACAGTAAAAA CAACAGCACA GATGCTGCAG 
AGCTTTGGAA CAGACCTCGC AGAGACTGAA CTTCCCAACG ACGTGCAGTG CACGGAGGAG 
CTGCTCTCCG CACACACAGA CCACCACGGC AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG 
AAGCAGGGGG CCACCCTGCT GACCTGCATC AGGGAGCCGG TCACCCGCAG CGCCAACAGC 
AAGCTCAGCC CTGACGAACT GGAGAACGTG GCAACAGTAG AGAGGTTACT GGCTCAGTTG 
GATGAAACGG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTGGAACAG 
TGCCTGCAGC TGCGACACTT TGAGCATGAT TTCAGAGAGG TAAAACTGGC ATTGGATAAT 
CTCATGGAAG GACAAGCAGG TTTTGCTGAC ATTGGAGACA GCGTGACTCG AGTGGAGCAT 
CTCCTCCGGG AACAGAAACA CCTGGAAGAG AAAGGACAGG AACCTTTGGA GAAGGCCCAG 
TCTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAATC ATTATGCAGT TGACTCCATT 
AGACCAAAGT GTGTTGAACT CAGGCGTATT TGTGATGACT TTACCAATGA GACCAAGAAA 
AAGTATGATA TTTTGGGAAA GTCTCTTGAG CTGCACAAGC AGTTAGATAA GGCAAGCCAA 
TGGTGTGAAG CTGGAATCTA CCTCTTGGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA 
GAGGGAGCTG AAACTGCACT CGTTGAGATT GAGAAGTTCC TGGTAACAGC CAAGGAGCAC 
CAGCTCTCCA ACCCGAAGGA GTTCTACAAT CAGTTTGACA TGATCCTCAC CCCTGAAATA 
AAGGCCAACG CCCAAAGAAT TCTACAGAAA CTAGAAGATG TACAAGAAAT GTTTGACAAG 
AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC AAGCAGACGC GGCCAGTGCA GCCTGTGGCC 
CCGCACCCCG AATCTTCCCC AAAGCGAGCC TCACCAAAGA TCACCAGACC AGCAGGAGTA 
GCTGCCAACA GGAGATCCAC AGAAATTTCC TGTCCTCCCA AGTCCATCTC TGAAGTCGAG 
TTATCAAAAA AGAAAAACAT TAAGAAGACT AAAGGTGGAA TTAAGATCGA AGTGATGCAC 
GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG CTGGTGATGA GCGAGAGCGA CGAGAACCTC 
TCCTCCAGGA GGAGCCACAT CATCAATGAG CTGATAGAGA CAGAACGGGT TTACGTAGAA 
GAACTTCAAA GTATAATAGA GGGATACGCC TCGGAAATGG ACAATCCCAG TTTGGTACAT 
CTGATCCCAG CTGCCCTCCA GAACAAGAAG GACATTCTTT TTGGGAACCT CCCAGAGATC 
TATGAATTCC ACAACAGGAT TTTCCTTAAG GAGATCGAAA ACTGCATTGA AAACCCTGAG 
CTTCTTGCCC GATGCTTTTT GAAAAGAAAG GAGGACCTCC AGATTTATGA GAAATACTGT 
CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTCCAG 
GAATGCCAGC GTAAATTGGA CCATAAGCTC TCCTTAGACG CTTATCTCTT AAAGCCGGTG 
CAGAGAATCA CCAAGTACCA GCTGCTGCTA AAGGAAATGC TGAAGTGCAG CAAGAATTCG 
GAGGGTACTG CTGAGCTGGA AGAAGCCCTG GCCACGATGC TTGATATCAT CAAGTCCGTG 
AACGACTCCA TGCACCAGAT AGCCATCACA GGATACGAGG GGGACGTTGG TGAGCTTGGC 
AAGTTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG GCACAACAAA 
GTGAAGGACT TGGCTAGGTT CAAACCCATG CAGAGACACC TCTTCCTCTA CACAAAGATG 
CTACTTTTCT GCAAGAAACG GGAGGAGAAC ACAGACGGAC ATGAGAAGAC AGCTTCATAC 
AGCTTTAAAA ATTCTTTAAA GATGAGCACT GTGGGCATTA CAGAAAATGT AAAAGGGGAT 
AACAAGAAAT TTGAGATCTG GTATAATGGC AGAGAAGAGG TCTATATCAT TCAGGCATCT 
TCTGTTGAGC TGAAGAACAC CTGGATTTCA GAGATTCGCA AAGTCCTGAC AGGACAACTG 
GAAGCGTGTA GAGAAGCAAG TCAACTGCAC CAAAGGATCA CCGAGAGCGT ATACCACGCA 
CCTATGGACT CCTCCAACCC AAGCAGGTAT AGCAGGAAGT CATCGAGTCT GTATGAAAAC 
AAGTCCGAGA CATCAAACGT GGAAGCATCT AACAACAAAA ACAGGAATTC CAGTCCCAGC 
GCCAGACAAA AGAGAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGGCA TGAAATAAAG 
AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATTTT GGCTCAGACT 
AAATGTAATA CCGGTTCCAC CACGAGGCCC GTCCCTAGAT CTGCTTCACA GACAGGCTGG 
CCCGGCAGGC AGATGCCATC TCTAGACACC TTTGAAGATT TTGAAGCCAT CCCCTCCAGC 
ACGGACGAGC TCTCCAACTC CTCGGACTTG GAGGAAGAGA CCAACCCCAA CAACGGGACC 
AATCTCTTTC GGGTGGAAGG CAGCTTTGAC AGCTGTTTCC CGGATTCTCT GACCCTGGAG 
GATGGAGATT TAGTGCAGTT TGTGCAGGAA GGAGAGAATG GTCAATGGCT GGTGAAGAAG 
CTGGTCTCTG AGAAGAGCGA CTGGGTTCCC TCCAGTATCT TGCAGCCCGC AGAGGGGGAC 
AGTCACACCA TCATTAAGAA CAGCAATGCA GACACGTACA ACGATTCCTG CAGCACGGCC 
TCCCTGGAGT CGGACACCAA GGAGAATGAG GTGGCCAAGA GCTTCCCAGC GGAGCAGAGG 
GCCGGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L2 ( XM_014939886.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795372.1 Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X7, mRNA.

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CloneID	OCa63501
Clone ID Related Accession (Same CDS sequence)	XM_014939886.1
Accession Version	XM_014939886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable guanine nucleotide exchange factor MCF2L2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGGTAC CGATCATCAT GTTGAATTCC GTCTCCGACC TGCACGGCTA CATCGACAAA 
AGCCAGCTGA CGCGGGAGCT GGGGGGAACC CTGGAGTATG GGCACAGCCA GTGGATCCAC 
CACCGCACGG CTATTGAAAA CTTTGCAATG ACAGTAAAAA CAACAGCACA GATGCTGCAG 
AGCTTTGGAA CAGACCTCGC AGAGACTGAA CTTCCCAACG ACGTGCAGTG CACGGAGGAG 
CTGCTCTCCG CACACACAGA CCACCACGGC AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG 
AAGCAGGGGG CCACCCTGCT GACCTGCATC AGGGAGCCGG TCACCCGCAG CGCCAACAGC 
AAGCTCAGCC CTGACGAACT GGAGAACGTG GCAACAGTAG AGAGGTTACT GGCTCAGTTG 
GATGAAACGG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTGGAACAG 
TGCCTGCAGC TGCGACACTT TGAGCATGAT TTCAGAGAGG TAAAACTGGC ATTGGATAAT 
CTCATGGAAG GACAAGCAGG TTTTGCTGAC ATTGGAGACA GCGTGACTCG AGTGGAGCAT 
CTCCTCCGGG AACAGAAACA CCTGGAAGAG AAAGGACAGG AACCTTTGGA GAAGGCCCAG 
TCTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAATC ATTATGCAGT TGACTCCATT 
AGACCAAAGT GTGTTGAACT CAGGCGTATT TGTGATGACT TTACCAATGA GACCAAGAAA 
AAGTATGATA TTTTGGGAAA GTCTCTTGAG CTGCACAAGC AGTTAGATAA GGCAAGCCAA 
TGGTGTGAAG CTGGAATCTA CCTCTTGGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA 
GAGGGAGCTG AAACTGCACT CGTTGAGATT GAGAAGTTCC TGGTAACAGC CAAGGAGCAC 
CAGCTCTCCA ACCCGAAGGA GTTCTACAAT CAGTTTGACA TGATCCTCAC CCCTGAAATA 
AAGGCCAACG CCCAAAGAAT TCTACAGAAA CTAGAAGATG TACAAGAAAT GTTTGACAAG 
AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC AAGCAGACGC GGCCAGTGCA GCCTGTGGCC 
CCGCACCCCG AATCTTCCCC AAAGCGAGCC TCACCAAAGA TCACCAGACC AGCAGGAGTA 
GCTGCCAACA GGAGATCCAC AGAAATTTCC TGTCCTCCCA AGTCCATCTC TGAAGTCGAG 
TTATCAAAAA AGAAAAACAT TAAGAAGACT AAAGGTGGAA TTAAGATCGA AGTGATGCAC 
GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG CTGGTGATGA GCGAGAGCGA CGAGAACCTC 
TCCTCCAGGA GGAGCCACAT CATCAATGAG CTGATAGAGA CAGAACGGGT TTACGTAGAA 
GAACTTCAAA GTATAATAGA GGGATACGCC TCGGAAATGG ACAATCCCAG TTTGGTACAT 
CTGATCCCAG CTGCCCTCCA GAACAAGAAG GACATTCTTT TTGGGAACCT CCCAGAGATC 
TATGAATTCC ACAACAGGAT TTTCCTTAAG GAGATCGAAA ACTGCATTGA AAACCCTGAG 
CTTCTTGCCC GATGCTTTTT GAAAAGAAAG GAGGACCTCC AGATTTATGA GAAATACTGT 
CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTCCAG 
GAATGCCAGC GTAAATTGGA CCATAAGCTC TCCTTAGACG CTTATCTCTT AAAGCCGGTG 
CAGAGAATCA CCAAGTACCA GCTGCTGCTA AAGGAAATGC TGAAGTGCAG CAAGAATTCG 
GAGGGTACTG CTGAGCTGGA AGAAGCCCTG GCCACGATGC TTGATATCAT CAAGTCCGTG 
AACGACTCCA TGCACCAGAT AGCCATCACA GGATACGAGG GGGACGTTGG TGAGCTTGGC 
AAGTTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG GCACAACAAA 
GTGAAGGACT TGGCTAGGTT CAAACCCATG CAGAGACACC TCTTCCTCTA CACAAAGATG 
CTACTTTTCT GCAAGAAACG GGAGGAGAAC ACAGACGGAC ATGAGAAGAC AGCTTCATAC 
AGCTTTAAAA ATTCTTTAAA GATGAGCACT GTGGGCATTA CAGAAAATGT AAAAGGGGAT 
AACAAGAAAT TTGAGATCTG GTATAATGGC AGAGAAGAGG TCTATATCAT TCAGGCATCT 
TCTGTTGAGC TGAAGAACAC CTGGATTTCA GAGATTCGCA AAGTCCTGAC AGGACAACTG 
GAAGCGTGTA GAGAAGCAAG TCAACTGCAC CAAAGGATCA CCGAGAGCGT ATACCACGCA 
CCTATGGACT CCTCCAACCC AAGCAGGTAT AGCAGGAAGT CATCGAGTCT GTATGAAAAC 
AAGTCCGAGA CATCAAACGT GGAAGCATCT AACAACAAAA ACAGGAATTC CAGTCCCAGC 
GCCAGACAAA AGAGAGGCTG GCCCGGCAGG CAGATGCCAT CTCTAGACAC CTTTGAAGAT 
TTTGAAGCCA TCCCCTCCAG CACGGACGAG CTCTCCAACT CCTCGGACTT GGAGGAAGAG 
ACCAACCCCA ACAACGGGAC CAATCTCTTT CGGGTGGAAG GCAGCTTTGA CAGCTGTTTC 
CCGGATTCTC TGACCCTGGA GGATGGAGAT TTAGTGCAGT TTGTGCAGGA AGGAGAGAAT 
GGTCAATGGC TGGTGAAGAA GCTGGTCTCT GAGAAGAGCG ACTGGGTTCC CTCCAGTATC 
TTGCAGCCCG CAGAGGGGGA CAGTCACACC ATCATTAAGA ACAGCAATGC AGACACGTAC 
AACGATTCCT GCAGCACGGC CTCCCTGGAG TCGGACACCA AGGAGAATGA GGTGGCCAAG 
AGCTTCCCAG CGGAGCAGAG GGCCGGCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795372.1
CDS	512..4024
Translation	MASRCRKCGQQVPRNPPFTAVRITEGEMQSYHDFIGGCIRLERMAKRDEIMQQEIRPLVAVDIIEQLHRQFAILSGGRGKDGAPIITFPEFAGFSDIPDEDFLNVVTYLTSIPSLEAASIGFIIIIDRRRDKWSAVKASLTRIAGAFPGNLQLVFVLRPSRFIQRTIADIGIKLYRDDFKMKVPIIMLNSVSDLHGYIDKSQLTRELGGTLEYGHSQWIHHRTAIENFAMTVKTTAQMLQSFGTDLAETELPNDVQCTEELLSAHTDHHGKLKDELKLAVKQGATLLTCIREPVTRSANSKLSPDELENVATVERLLAQLDETEKAFDQFWTKHHLKLEQCLQLRHFEHDFREVKLALDNLMEGQAGFADIGDSVTRVEHLLREQKHLEEKGQEPLEKAQSLALHGEQLIQNNHYAVDSIRPKCVELRRICDDFTNETKKKYDILGKSLELHKQLDKASQWCEAGIYLLASQAVDKCQSQEGAETALVEIEKFLVTAKEHQLSNPKEFYNQFDMILTPEIKANAQRILQKLEDVQEMFDKRQVSLKKLAAKQTRPVQPVAPHPESSPKRASPKITRPAGVAANRRSTEISCPPKSISEVELSKKKNIKKTKGGIKIEVMHEASQGGSSRVLVMSESDENLSSRRSHIINELIETERVYVEELQSIIEGYASEMDNPSLVHLIPAALQNKKDILFGNLPEIYEFHNRIFLKEIENCIENPELLARCFLKRKEDLQIYEKYCQNKPRSEALWRQCGDSIFFQECQRKLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATMLDIIKSVNDSMHQIAITGYEGDVGELGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYTKMLLFCKKREENTDGHEKTASYSFKNSLKMSTVGITENVKGDNKKFEIWYNGREEVYIIQASSVELKNTWISEIRKVLTGQLEACREASQLHQRITESVYHAPMDSSNPSRYSRKSSSLYENKSETSNVEASNNKNRNSSPSARQKRGWPGRQMPSLDTFEDFEAIPSSTDELSNSSDLEEETNPNNGTNLFRVEGSFDSCFPDSLTLEDGDLVQFVQEGENGQWLVKKLVSEKSDWVPSSILQPAEGDSHTIIKNSNADTYNDSCSTASLESDTKENEVAKSFPAEQRAGS

Target ORF information:

RefSeq Version	XM_014939886.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939886.1

ORF Insert Sequence:

ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAACCCACC CTTCACGGCC 
GTCCGGATAA CAGAAGGGGA GATGCAGTCT TACCATGATT TTATAGGAGG ATGCATTAGA 
CTGGAGCGCA TGGCAAAGAG AGATGAAATC ATGCAGCAGG AGATACGGCC GCTGGTTGCA 
GTGGATATCA TAGAGCAGCT CCACAGGCAG TTTGCTATCT TGTCAGGAGG AAGAGGGAAG 
GATGGGGCGC CCATTATAAC CTTTCCAGAA TTTGCGGGAT TCAGTGACAT ACCTGATGAA 
GATTTTCTGA ACGTGGTCAC GTATCTCACC AGCATCCCCA GTCTGGAGGC TGCCAGCATC 
GGGTTCATCA TCATCATAGA CAGACGACGG GACAAATGGA GCGCAGTCAA GGCATCCCTG 
ACACGGATAG CAGGAGCGTT TCCAGGGAAC CTGCAGCTGG TGTTTGTGCT GCGACCCTCC 
CGCTTCATCC AAAGGACCAT CGCTGACATT GGCATTAAGC TCTATCGAGA TGACTTTAAA 
ATGAAGGTAC CGATCATCAT GTTGAATTCC GTCTCCGACC TGCACGGCTA CATCGACAAA 
AGCCAGCTGA CGCGGGAGCT GGGGGGAACC CTGGAGTATG GGCACAGCCA GTGGATCCAC 
CACCGCACGG CTATTGAAAA CTTTGCAATG ACAGTAAAAA CAACAGCACA GATGCTGCAG 
AGCTTTGGAA CAGACCTCGC AGAGACTGAA CTTCCCAACG ACGTGCAGTG CACGGAGGAG 
CTGCTCTCCG CACACACAGA CCACCACGGC AAGCTGAAGG ACGAGCTGAA GCTGGCTGTG 
AAGCAGGGGG CCACCCTGCT GACCTGCATC AGGGAGCCGG TCACCCGCAG CGCCAACAGC 
AAGCTCAGCC CTGACGAACT GGAGAACGTG GCAACAGTAG AGAGGTTACT GGCTCAGTTG 
GATGAAACGG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTGGAACAG 
TGCCTGCAGC TGCGACACTT TGAGCATGAT TTCAGAGAGG TAAAACTGGC ATTGGATAAT 
CTCATGGAAG GACAAGCAGG TTTTGCTGAC ATTGGAGACA GCGTGACTCG AGTGGAGCAT 
CTCCTCCGGG AACAGAAACA CCTGGAAGAG AAAGGACAGG AACCTTTGGA GAAGGCCCAG 
TCTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAATC ATTATGCAGT TGACTCCATT 
AGACCAAAGT GTGTTGAACT CAGGCGTATT TGTGATGACT TTACCAATGA GACCAAGAAA 
AAGTATGATA TTTTGGGAAA GTCTCTTGAG CTGCACAAGC AGTTAGATAA GGCAAGCCAA 
TGGTGTGAAG CTGGAATCTA CCTCTTGGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA 
GAGGGAGCTG AAACTGCACT CGTTGAGATT GAGAAGTTCC TGGTAACAGC CAAGGAGCAC 
CAGCTCTCCA ACCCGAAGGA GTTCTACAAT CAGTTTGACA TGATCCTCAC CCCTGAAATA 
AAGGCCAACG CCCAAAGAAT TCTACAGAAA CTAGAAGATG TACAAGAAAT GTTTGACAAG 
AGGCAAGTGA GCCTGAAGAA GCTGGCAGCC AAGCAGACGC GGCCAGTGCA GCCTGTGGCC 
CCGCACCCCG AATCTTCCCC AAAGCGAGCC TCACCAAAGA TCACCAGACC AGCAGGAGTA 
GCTGCCAACA GGAGATCCAC AGAAATTTCC TGTCCTCCCA AGTCCATCTC TGAAGTCGAG 
TTATCAAAAA AGAAAAACAT TAAGAAGACT AAAGGTGGAA TTAAGATCGA AGTGATGCAC 
GAGGCCAGCC AGGGAGGGTC CAGCAGGGTG CTGGTGATGA GCGAGAGCGA CGAGAACCTC 
TCCTCCAGGA GGAGCCACAT CATCAATGAG CTGATAGAGA CAGAACGGGT TTACGTAGAA 
GAACTTCAAA GTATAATAGA GGGATACGCC TCGGAAATGG ACAATCCCAG TTTGGTACAT 
CTGATCCCAG CTGCCCTCCA GAACAAGAAG GACATTCTTT TTGGGAACCT CCCAGAGATC 
TATGAATTCC ACAACAGGAT TTTCCTTAAG GAGATCGAAA ACTGCATTGA AAACCCTGAG 
CTTCTTGCCC GATGCTTTTT GAAAAGAAAG GAGGACCTCC AGATTTATGA GAAATACTGT 
CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTCCAG 
GAATGCCAGC GTAAATTGGA CCATAAGCTC TCCTTAGACG CTTATCTCTT AAAGCCGGTG 
CAGAGAATCA CCAAGTACCA GCTGCTGCTA AAGGAAATGC TGAAGTGCAG CAAGAATTCG 
GAGGGTACTG CTGAGCTGGA AGAAGCCCTG GCCACGATGC TTGATATCAT CAAGTCCGTG 
AACGACTCCA TGCACCAGAT AGCCATCACA GGATACGAGG GGGACGTTGG TGAGCTTGGC 
AAGTTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG GCACAACAAA 
GTGAAGGACT TGGCTAGGTT CAAACCCATG CAGAGACACC TCTTCCTCTA CACAAAGATG 
CTACTTTTCT GCAAGAAACG GGAGGAGAAC ACAGACGGAC ATGAGAAGAC AGCTTCATAC 
AGCTTTAAAA ATTCTTTAAA GATGAGCACT GTGGGCATTA CAGAAAATGT AAAAGGGGAT 
AACAAGAAAT TTGAGATCTG GTATAATGGC AGAGAAGAGG TCTATATCAT TCAGGCATCT 
TCTGTTGAGC TGAAGAACAC CTGGATTTCA GAGATTCGCA AAGTCCTGAC AGGACAACTG 
GAAGCGTGTA GAGAAGCAAG TCAACTGCAC CAAAGGATCA CCGAGAGCGT ATACCACGCA 
CCTATGGACT CCTCCAACCC AAGCAGGTAT AGCAGGAAGT CATCGAGTCT GTATGAAAAC 
AAGTCCGAGA CATCAAACGT GGAAGCATCT AACAACAAAA ACAGGAATTC CAGTCCCAGC 
GCCAGACAAA AGAGAGGCTG GCCCGGCAGG CAGATGCCAT CTCTAGACAC CTTTGAAGAT 
TTTGAAGCCA TCCCCTCCAG CACGGACGAG CTCTCCAACT CCTCGGACTT GGAGGAAGAG 
ACCAACCCCA ACAACGGGAC CAATCTCTTT CGGGTGGAAG GCAGCTTTGA CAGCTGTTTC 
CCGGATTCTC TGACCCTGGA GGATGGAGAT TTAGTGCAGT TTGTGCAGGA AGGAGAGAAT 
GGTCAATGGC TGGTGAAGAA GCTGGTCTCT GAGAAGAGCG ACTGGGTTCC CTCCAGTATC 
TTGCAGCCCG CAGAGGGGGA CAGTCACACC ATCATTAAGA ACAGCAATGC AGACACGTAC 
AACGATTCCT GCAGCACGGC CTCCCTGGAG TCGGACACCA AGGAGAATGA GGTGGCCAAG 
AGCTTCCCAG CGGAGCAGAG GGCCGGCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MCF2L2 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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