JMJD1C cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	JMJD1C
Entrez Gene ID	106887103
Full Name	jumonji domain containing 1C
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014940495.1	XP_014795981.1	probable JmjC domain-containing histone demethylation protein 2C isoform X2
XM_014940493.1	XP_014795979.1	probable JmjC domain-containing histone demethylation protein 2C isoform X1

Pathways from BioSystems
Gene Ontology

The following JMJD1C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the JMJD1C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa63957	XM_014940493.1 Latest version!	Calidris pugnax jumonji domain containing 1C (JMJD1C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCa63958	XM_014940495.1 Latest version!	Calidris pugnax jumonji domain containing 1C (JMJD1C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

JMJD1C ( XM_014940493.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795979.1 Calidris pugnax jumonji domain containing 1C (JMJD1C), transcript variant X1, mRNA.

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CloneID	OCa63957
Clone ID Related Accession (Same CDS sequence)	XM_014940493.1
Accession Version	XM_014940493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable JmjC domain-containing histone demethylation protein 2C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090828.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGTC CCTACTCCTT AAATGGTTAT AGGGTGAGAG TGTACAGACA GGATTCTGCC 
ACCCAGTGGT TCACTGGTAT CATTACCCAT CATGATCTCT TCACCCGCAC TATGGTTGTT 
ATGAATGACC AGGTGTTAGA ACCTCAGAAT GTTGATCCTT CTATGGTTCA GATGACCTTT 
CTAGATGATG TTGTTCACTC TTTGTTGAAA GGTGAAAATA TTGGCATCAC TTCACGCCGA 
CGGTCTCGTT CCAACCAGAA CAGCAACACA GTCCACGGTC ATTATACACG TGCGCAAGCA 
AATAGTCCCA GACCAGCAAT GAATTCCCAA ACTGCAGCAC CAAAACAAAA TTCTCACCAG 
CACCAGCAAC AGAGGAATAC TCGGCCGAAT AAGAGGAAAG GCTCTGATAG CAGCATGCCT 
GATGAAGAGA AGATGAAAGA AGAAAGATAC GATTATATAG GTCGAGGAGA AAACCCCAAA 
AACAAAAATA AACACTTCCT TAGTAAAAGA AGAAAACCTG AAGAGGATGA AAAAAAGTTA 
AATATGAAGA GACTGCGGAC AGACAATATC TCAGATTACT CTGAGAGCAG TGACTCAGAA 
ATTTCAAATA AAAGATTAAC AGATTCGTCC TCAGAGCAAA ATTCGGAAAA TGAGTTAAAA 
AGCAAGAACA CTTCAAAGAT AAATGGAGAA GAAGGAAAAT CCCAAACTGT TGAGGGAGTA 
GAACAGACAC TAACAGATAG GCGGTCTCCA TGGGATGAAA TACAGGAGGA TAAAAAACAC 
GAAGAGACAG AAAGACTTAA GTCAGCCGTC TTAGATCAGC AGGAAAAATC TTCACTCCAT 
GCAGCAGAGC AGCCAACACC TTATGAGCAG AATGCCAAGG ATCCACATGT TCAAGAGTGC 
AATGCAGAAA AACATCATAC TGCGGAATTA AAAAATGAGA AGTTTGTACC CGGACCTCCT 
ACTCCGAAAT GTGTTGTTGA CATTACTAAT GAAAACAACT CTGAAAAGGA GAGCCAGGAT 
AATGCTGCAA GTACCTTTGG TTTGCAAACG GTTCAGAAAG GGGAATCTCA CAGCAGCGAT 
TTAAAACAGC AGTTTGCAAA TGCAAATTTC CTTGAAACAA GAAAACAGGA AGCTGATCAA 
AGTTGGGTTA GCAGTGTCGT TAGCAAAACG GATTTCATAC AACCTGGAGT TGTAAAAACA 
TTATCAGTAA ATGAACACTT AAATCCTGAA AAAGAAAAAG TGCAGTATGG CTCCTTTATG 
TCTTCGTTAA ATGTAGCTTC TCTAGCAGAA GAGAGTAAAC TTCATAAACA AAGTCCAGTC 
CCCGATTCTG TGAAGTCAAA ACCTAGTGCT TCAGTTGATC ATCCTAAAAC AAAGTCACCT 
GATGTTAAAC CTAAATTCAC CCAATCTTCT GATACTGTGA AGTCTAAGGT TAGTTCCCAA 
AACAGCCATG CTACTGGATT AACAAGGTCA GCCAATAAAA CTGATCATGA TTTGCCTAGG 
TCTAGTTTTC ATCCAGTTCC AGCTAGAGTT AGCGCTCTAG AAGCTACTAA AAGTCCTCTT 
ATCATTGACA AGAATGAACA TTTCACGGTG TACAGGGACC CCACTCTGAT TGGACAAGAA 
ACAGGAACTA ATCACATTTC ACCTTATTTG CATCAGCATA ATTATCCTCT GCATTCCTCA 
TCCCACCGAA CCTGTTTAAA TCCAAACGCT CATCATCCTG CATTAACTGG TTCATCCCAT 
CTGCTAGCTG GGTCTTCAGC TCAGGCTCCC CTGTCCGCTA TTAACACTCA CCCCCTTAGT 
ACCGCATCCC ACCATTCTGT TCATCACCCT CATCTACTTC CTGCAGTGTT ACCCGGAGTG 
CCTGCTGCCT CCTTGCTGGG TGGCCACCCG CGACTAGAGA CTGCTCATGC TAGCAGCTTA 
AGCCATTTGG CATTAGCACA CCAGCAGCAG CAACAGATGT TACAACACCA GTCTCCACAT 
CTTCTTGGAC AAGCTCATCC TTCTGCTTCC TATAATCAGC TAGGGCTTTA TCCAATTATT 
TGGCAGTATC CAAATGGAAC ACATGCTTAC TCAGGACTCG GTCTGCCCTC CTCCAAATGG 
GTCCACCCAG AAACTCCTGT TAACGCGGAG GCTTCCTTGA GAAGGAATAC TCCCAGCCCA 
TGGTTACACC AGCCCACCCC TGTGACCTCA GCTGACAGCC TCGGTTTACT GAGTCACATT 
CCTGTGCGAC CGTCCAGTGC AGATCCCCTT CGGCCTCTCA AACTGACAAC GCATTCCAGC 
CCACCATTGT CCAAAAGTAT CATAGAGCAT CACAAAGAAG AACTGGAGAG GAAAGCGTTT 
ATTGAACCTT TACGTTCTGT TACAACTGCG CCAGTAAAGA CGGAGCTAGA GCAGAGCAGA 
ACACAGACGG CGAAGGAGAG CCATATGCAC AGACATTTTG CAGATCCAAT GTTGAACCAG 
CTGCCAAGGC CACCACAGGA GACTGGGGAG CGACTGAGCA AATACAAAGA AGAACACCGA 
CGGATACTCC AAGAAAGTAT TGAAGTTGCT CCTTTTACAG CTAAAATAAA GGCACTGGAG 
GGAGAGAGAG AGAACTATTC CAGGGTTGCG TCCTTATCTT CAAGTCCCAA AAGCCATTCG 
GTAAAATATG ACAAAGATGC CGAACGCTCT GTGTCAGAGC TGTACAAAAT GAAGCATTCG 
GTTCCACAGA GTTTACCACA AAGTAACTAC TTCACCACCT TGTCTAACAG TGTAGTAAAT 
GAACCGCCGA GATCCTACCC ATCAAAGGAG ACTTCAAGCG CGTATGTTGA TAAGCAGACT 
AATTGTGCTT CAACAGCAGC TAGTCCCCAG TCTCTCCCCT CTTACATTTC TTCTCTTTCA 
AAACCTCCAC CTTTAATTAA GCACCAACCA GAGAGCGAAG GCTCTGCGAG CAAGATACCT 
GAGCAGCTCT CGCAGTCAGT GCAGTCTCAC TCTGTAAATT CTTTTAGAAG CGACAGCAGG 
AGCCCTACTC AGTTGACAGT CTCCTCTTCA AATACACTCC GGAGTATGCC TGCCTTGCAT 
AGAGCCCCTG TGTTTCACCC CCCTGTGCAC CAGAACCTGG AGAAGAAGGA AAGCAGCTAT 
AGCAGCCTTT CACCTCCAAC TCTGACTCCC GTTCAGCCTG TTAATGCTGG TGGTGGCAAA 
ATACAGGAGT TGCAGAAACC ACCAACTCTA GTACCTGAGC CTAAGGAAGC TCAGACTGTT 
TACAAGGGCA CTTCTGAACA GAATGTATCA GAGGCATGGA AGTCCAATAG TGCCCCAAAT 
AACGAAAAAG TGGATTGGCA CGTTGAAAGA ATGAGTGGAA AGTCACAGTC CGCTACAGCA 
TCTGTTATTG TGCGTCCTCC TTCTAGCACG AAATACGATA GCGTAGCAGT AATGCAGTCT 
GCTTCCAAAG ACCGAGGCAG TGAGAGATCT TCAGCTGTGA CAAATCAAGC AGATTGCCTG 
AAAACAGCGG AAGCCAGGGA GACTGGAAGA ATCATTCTGC CAAATATGAA CTCAGACAGC 
GCTCGCACAC AGTATGAAAA GAAATTTCCA GCTGTCTCGC AAGGCAGCAT TCCGTGTGCT 
GTCACCCCTA CCACAACGAT GATCTGCAGT ACCAAAACGG ATGTCACCGC ATCGGCAGCA 
ACAACTACCA GCGTGTCGAG CTGGGGTAGT TCAGAGGTGA CTTACTCCTT ATCGAACACG 
GTCCTGGCCT GCGCGCCGCT GGAGTGTACG GCCTCGAGAG CCGCAAGTCA GGCGGTGGCA 
CAGGCCCAGG AATGCAAGGT CAGCACTCCG GCTCCGGTTA CACCCGCCGC TGGCAAGGCA 
GGCGGTGGTG CTCAGCCCAG CTCGGGATTC TCCACCTCTA CTGACTTTGT CCATTTGAAA 
AAGCACAAGG CGGCCTTGGC TGCAGCTCAG TTTAAAAGTA GTAACACCAG TGAGACCGAG 
TCCAACTCTG TGAAAAATCA GACATTTTCA ACCTCTCTCT CCCTAGACAG CACTATCGTC 
TGTAATACAA TAAACAAAGC GAACTCTGTA GGCAGTGGGC AAACTTCCCA GACGAGTCAG 
CCAAACTACC ACACTAAACT GAAAAAGGCT TGGCTAACAA GACATTCGGA GGAAGATAAA 
AACACTAATA AAAAAGAGAA TTCAGGGAAC AGTGTCTCAG AAATTATTAA GCCATGTACT 
GTCAATTTAA TAGCTTCTAC ATCAAATGAT TTGCAAAATA ACATAGACGG TAAAATCTTG 
GCAGATAAGT TTGCGAAGGA AGATAAGCAC CCTCGGAGAA AAGCAAAACG AACTTACGAG 
TCTGGCTCTG AAAGTGGAGA CTCTGATGAA AGCGAGAGCA AGTCAGAGCA AAGGACTAAA 
CGGCAGCCCA AGCCAACTTA CAAAAAGAAA CAAAATGATT TGCAGAAAAA AAAGGGTGAT 
ACGGAGGAAG AAGTAAAACC GAATGGTGTT CTTAGCAGGA GTGCCAAAGA GAAAAGCAAG 
CTGAAGTTAC AGAGCGGCAG TAACAGCACT GGTATACCTC GCTCAGTGTT AAAAGACTGG 
CGCAAAGTAA AGAAGCTGAA GCAAACAGGA GAGTCCTTTT TGCAGGATGA TTCTTGCTCT 
GAAATAGGAC CAAATTTGCA AAAATGCAGA GAATGTAGGT TAATAAGAAG TAAGAAAGGA 
GAAGAACCAA CTCACTCACC AGTATTTTGT AGATTTTACT ACTTTCGGCG GTTATCTTTC 
AGTAAGAATG GAGTGGTTAG GATAGATGGT TTCTCCTCTC CTGATCAATA TGATGATGAA 
GCACTAAGTT TATGGACGCA TGAAAACTAT GAAGATGATG AACTGGACCT AGAAACTTCT 
AAATACATTC TAGATATCAT AGGTGATAAA TTTTGTCAGT TGGTAACGTC TGAGAAAACA 
GCCATGTCCT GGGTGAAGAA AGACGCCAAA ATTGCATGGA AGAGAGCAGT GAGAGGAGTC 
CGTGAGATGT GTGATGCATG TGAAGCCACA TTATTTAACA TTCATTGGGT CTGCCAAAAA 
TGTGGATTTG TGGTCTGCCT AGATTGTTAC AAAGCAAAGG AAAGAAAAAG TTCTAGAGAT 
AAAGAGTTAT ATGCCTGGAT GAAGTGTGTG AAGGGACAGC CTCACGATCA CAAACACCTG 
ATGCCAACAC AGATTATTCC AGGCTCTGTT TTGACAGACC TTCTAGATGC TATGCACAGT 
ATTAGAGAAA AATTTGAAAT TAAATCCCAT TGTCAGTGTA CCAGCAAGCA GAGCACACAA 
GCTGGCAAGC TCCCTGCAAT GAATGGTGTG TCTCAGGTTT TGCAGAATGT CCTCAACCAC 
AGTAATAAAA TTTCTCTGTG CATGCCTGAG TCTCAACAGC AGAATACTCC TCAAAAGTCT 
GAGACAAATG GTAATACAAG TCCTAGAAGC GATGTAAGCA CAGACAGCAA GTTAACACCT 
CCTGAATCCC AGTCACCGCT GCACTGGTTA GCTGATCTTG CAGAGCAGAA AGCCAGAGAA 
GAAAAGAAAG AGAACAAGGA ATGTCCTTCT GGAAAACATT CAAAGGAAGA TAAAGACCAG 
GATAATTTGG AGTCCCAGAA CTGTAAAAGC TCCCCTCCTG CCTCCCAGAA CAACGAGCAG 
GGCTCAACCT TACGAGATTT ATTAACTACA ACAGCAGGCA AACTGCGCCT GGGTTCTACA 
GATGCTGGGA TTGCTTTTGC TCCAGTTTAC TCTACAGGAA CAGCAAGTGG CAAGAGTGGA 
AGGACTATGC CAAACATTCT TGATGATATA ATTGCTTCAG TAGTAGAAAA CAAGATTCCA 
CCAAATAGAG CACCAAAGAT AAATGTAAAA TCTGAAATAA AAGACGAGCC AAAGGACGAC 
AAAAAATGTG TTCAGGATGA CTGCAGTAAA CTGTACAGTG ATATTCAGTA TTCATGGATC 
TGTGATAAGC ATGTTTTGTG GCTCAGAGAC CATAAAAACA ACAACAACGG GAAGCTCTTC 
AAGGAGTGTT GGAGACAAGG AAGGCCTGTT CTAGTGTCCG GTATGCATAA GAAAATGAAC 
TTCAGCCTGT GGAAAGCTGA GTCAATTAGT CTAGACTTTG GAAACCAGCA AGCTGATATC 
TTGAATTGCA AAGACAGTAT TATTTCAAAC ACCAATGTCA AGGAGTTCTG GGATGGTTTT 
GAAGATGTTT CAAAACGGCA GAAAATAAAA AATGGGGAAA CAGCTCTACT GAAACTGAAA 
GACTGGCCTT CTGGTGAAGA TTTCAAGGCT ATGATGCCAG CAAGGTATGA GGACTTATTA 
AAAAGTTTAC CCTTGCCTGA ATACTGTAGT CCAGAAGGAA AGCTAAACCT GGCTTCTCAT 
CTGCCGGGAT TTTTTGTCCG TCCGGATTTG GGACCCAGAC TGTGCAGTGC CTATGGTGTG 
GCTGCGACTA AAGACCATGA TATAGGAACC ACAAATCTCC ATATTGAAGT TTCTGATGTT 
GTGAACATCC TTGTTTACGT TGGCATAGCA AAAGGAAATG GAGTGCTTTC CAAATCAGGA 
GTTTTGAAGA AGTTTGAAGA GGAAGATTTG GATGACCTTT TAAGGAAGCG GTTGAAAGAT 
TCAAGTGAAT TACCTGGTGC TTTATGGCAC ATTTATGCTG GCAAAGACGC TGACAAGATA 
AGGGAGTTTC TGCAAAAGAT AGCAAAAGAG CAAGGCTTAG AAGTTTTACC GGAGCATGAT 
CCGATACGTG ATCAGAGTTG GTACGTGAAC AAAAAACTTC GCCAAAGACT TCTTGAAGAA 
TATGGAGTGA AAACCTGCAC GGTTATTCAG TTTCTTGGTG ATGCTATTAT TCTACCAGCA 
GGAGCACTTC ATCAGGTGCA AAATTTTCAC AGCTGCGTTC AAGTAACTGA AGACTTTGTG 
TCTCCAGAAC ATCTTGTACA GTCCTTTCAC CTAACGCAGG AGCTGCGGCT GTCAAAGGAA 
GAAATCAATT ATGATGATAA ACTGCAGGTT AAAAATATCT TGTATCATGC AGTTAAAGAA 
ATGGTGAGAG CTCTGAAGAT CCATGAAGGT GAAATGGAAG ATATGGATGA AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795979.1
CDS	255..7331
Translation	MQGPYSLNGYRVRVYRQDSATQWFTGIITHHDLFTRTMVVMNDQVLEPQNVDPSMVQMTFLDDVVHSLLKGENIGITSRRRSRSNQNSNTVHGHYTRAQANSPRPAMNSQTAAPKQNSHQHQQQRNTRPNKRKGSDSSMPDEEKMKEERYDYIGRGENPKNKNKHFLSKRRKPEEDEKKLNMKRLRTDNISDYSESSDSEISNKRLTDSSSEQNSENELKSKNTSKINGEEGKSQTVEGVEQTLTDRRSPWDEIQEDKKHEETERLKSAVLDQQEKSSLHAAEQPTPYEQNAKDPHVQECNAEKHHTAELKNEKFVPGPPTPKCVVDITNENNSEKESQDNAASTFGLQTVQKGESHSSDLKQQFANANFLETRKQEADQSWVSSVVSKTDFIQPGVVKTLSVNEHLNPEKEKVQYGSFMSSLNVASLAEESKLHKQSPVPDSVKSKPSASVDHPKTKSPDVKPKFTQSSDTVKSKVSSQNSHATGLTRSANKTDHDLPRSSFHPVPARVSALEATKSPLIIDKNEHFTVYRDPTLIGQETGTNHISPYLHQHNYPLHSSSHRTCLNPNAHHPALTGSSHLLAGSSAQAPLSAINTHPLSTASHHSVHHPHLLPAVLPGVPAASLLGGHPRLETAHASSLSHLALAHQQQQQMLQHQSPHLLGQAHPSASYNQLGLYPIIWQYPNGTHAYSGLGLPSSKWVHPETPVNAEASLRRNTPSPWLHQPTPVTSADSLGLLSHIPVRPSSADPLRPLKLTTHSSPPLSKSIIEHHKEELERKAFIEPLRSVTTAPVKTELEQSRTQTAKESHMHRHFADPMLNQLPRPPQETGERLSKYKEEHRRILQESIEVAPFTAKIKALEGERENYSRVASLSSSPKSHSVKYDKDAERSVSELYKMKHSVPQSLPQSNYFTTLSNSVVNEPPRSYPSKETSSAYVDKQTNCASTAASPQSLPSYISSLSKPPPLIKHQPESEGSASKIPEQLSQSVQSHSVNSFRSDSRSPTQLTVSSSNTLRSMPALHRAPVFHPPVHQNLEKKESSYSSLSPPTLTPVQPVNAGGGKIQELQKPPTLVPEPKEAQTVYKGTSEQNVSEAWKSNSAPNNEKVDWHVERMSGKSQSATASVIVRPPSSTKYDSVAVMQSASKDRGSERSSAVTNQADCLKTAEARETGRIILPNMNSDSARTQYEKKFPAVSQGSIPCAVTPTTTMICSTKTDVTASAATTTSVSSWGSSEVTYSLSNTVLACAPLECTASRAASQAVAQAQECKVSTPAPVTPAAGKAGGGAQPSSGFSTSTDFVHLKKHKAALAAAQFKSSNTSETESNSVKNQTFSTSLSLDSTIVCNTINKANSVGSGQTSQTSQPNYHTKLKKAWLTRHSEEDKNTNKKENSGNSVSEIIKPCTVNLIASTSNDLQNNIDGKILADKFAKEDKHPRRKAKRTYESGSESGDSDESESKSEQRTKRQPKPTYKKKQNDLQKKKGDTEEEVKPNGVLSRSAKEKSKLKLQSGSNSTGIPRSVLKDWRKVKKLKQTGESFLQDDSCSEIGPNLQKCRECRLIRSKKGEEPTHSPVFCRFYYFRRLSFSKNGVVRIDGFSSPDQYDDEALSLWTHENYEDDELDLETSKYILDIIGDKFCQLVTSEKTAMSWVKKDAKIAWKRAVRGVREMCDACEATLFNIHWVCQKCGFVVCLDCYKAKERKSSRDKELYAWMKCVKGQPHDHKHLMPTQIIPGSVLTDLLDAMHSIREKFEIKSHCQCTSKQSTQAGKLPAMNGVSQVLQNVLNHSNKISLCMPESQQQNTPQKSETNGNTSPRSDVSTDSKLTPPESQSPLHWLADLAEQKAREEKKENKECPSGKHSKEDKDQDNLESQNCKSSPPASQNNEQGSTLRDLLTTTAGKLRLGSTDAGIAFAPVYSTGTASGKSGRTMPNILDDIIASVVENKIPPNRAPKINVKSEIKDEPKDDKKCVQDDCSKLYSDIQYSWICDKHVLWLRDHKNNNNGKLFKECWRQGRPVLVSGMHKKMNFSLWKAESISLDFGNQQADILNCKDSIISNTNVKEFWDGFEDVSKRQKIKNGETALLKLKDWPSGEDFKAMMPARYEDLLKSLPLPEYCSPEGKLNLASHLPGFFVRPDLGPRLCSAYGVAATKDHDIGTTNLHIEVSDVVNILVYVGIAKGNGVLSKSGVLKKFEEEDLDDLLRKRLKDSSELPGALWHIYAGKDADKIREFLQKIAKEQGLEVLPEHDPIRDQSWYVNKKLRQRLLEEYGVKTCTVIQFLGDAIILPAGALHQVQNFHSCVQVTEDFVSPEHLVQSFHLTQELRLSKEEINYDDKLQVKNILYHAVKEMVRALKIHEGEMEDMDEN

Target ORF information:

RefSeq Version	XM_014940493.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax jumonji domain containing 1C (JMJD1C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014940493.1

ORF Insert Sequence:

ATGCAAGGTC CCTACTCCTT AAATGGTTAT AGGGTGAGAG TGTACAGACA GGATTCTGCC 
ACCCAGTGGT TCACTGGTAT CATTACCCAT CATGATCTCT TCACCCGCAC TATGGTTGTT 
ATGAATGACC AGGTGTTAGA ACCTCAGAAT GTTGATCCTT CTATGGTTCA GATGACCTTT 
CTAGATGATG TTGTTCACTC TTTGTTGAAA GGTGAAAATA TTGGCATCAC TTCACGCCGA 
CGGTCTCGTT CCAACCAGAA CAGCAACACA GTCCACGGTC ATTATACACG TGCGCAAGCA 
AATAGTCCCA GACCAGCAAT GAATTCCCAA ACTGCAGCAC CAAAACAAAA TTCTCACCAG 
CACCAGCAAC AGAGGAATAC TCGGCCGAAT AAGAGGAAAG GCTCTGATAG CAGCATGCCT 
GATGAAGAGA AGATGAAAGA AGAAAGATAC GATTATATAG GTCGAGGAGA AAACCCCAAA 
AACAAAAATA AACACTTCCT TAGTAAAAGA AGAAAACCTG AAGAGGATGA AAAAAAGTTA 
AATATGAAGA GACTGCGGAC AGACAATATC TCAGATTACT CTGAGAGCAG TGACTCAGAA 
ATTTCAAATA AAAGATTAAC AGATTCGTCC TCAGAGCAAA ATTCGGAAAA TGAGTTAAAA 
AGCAAGAACA CTTCAAAGAT AAATGGAGAA GAAGGAAAAT CCCAAACTGT TGAGGGAGTA 
GAACAGACAC TAACAGATAG GCGGTCTCCA TGGGATGAAA TACAGGAGGA TAAAAAACAC 
GAAGAGACAG AAAGACTTAA GTCAGCCGTC TTAGATCAGC AGGAAAAATC TTCACTCCAT 
GCAGCAGAGC AGCCAACACC TTATGAGCAG AATGCCAAGG ATCCACATGT TCAAGAGTGC 
AATGCAGAAA AACATCATAC TGCGGAATTA AAAAATGAGA AGTTTGTACC CGGACCTCCT 
ACTCCGAAAT GTGTTGTTGA CATTACTAAT GAAAACAACT CTGAAAAGGA GAGCCAGGAT 
AATGCTGCAA GTACCTTTGG TTTGCAAACG GTTCAGAAAG GGGAATCTCA CAGCAGCGAT 
TTAAAACAGC AGTTTGCAAA TGCAAATTTC CTTGAAACAA GAAAACAGGA AGCTGATCAA 
AGTTGGGTTA GCAGTGTCGT TAGCAAAACG GATTTCATAC AACCTGGAGT TGTAAAAACA 
TTATCAGTAA ATGAACACTT AAATCCTGAA AAAGAAAAAG TGCAGTATGG CTCCTTTATG 
TCTTCGTTAA ATGTAGCTTC TCTAGCAGAA GAGAGTAAAC TTCATAAACA AAGTCCAGTC 
CCCGATTCTG TGAAGTCAAA ACCTAGTGCT TCAGTTGATC ATCCTAAAAC AAAGTCACCT 
GATGTTAAAC CTAAATTCAC CCAATCTTCT GATACTGTGA AGTCTAAGGT TAGTTCCCAA 
AACAGCCATG CTACTGGATT AACAAGGTCA GCCAATAAAA CTGATCATGA TTTGCCTAGG 
TCTAGTTTTC ATCCAGTTCC AGCTAGAGTT AGCGCTCTAG AAGCTACTAA AAGTCCTCTT 
ATCATTGACA AGAATGAACA TTTCACGGTG TACAGGGACC CCACTCTGAT TGGACAAGAA 
ACAGGAACTA ATCACATTTC ACCTTATTTG CATCAGCATA ATTATCCTCT GCATTCCTCA 
TCCCACCGAA CCTGTTTAAA TCCAAACGCT CATCATCCTG CATTAACTGG TTCATCCCAT 
CTGCTAGCTG GGTCTTCAGC TCAGGCTCCC CTGTCCGCTA TTAACACTCA CCCCCTTAGT 
ACCGCATCCC ACCATTCTGT TCATCACCCT CATCTACTTC CTGCAGTGTT ACCCGGAGTG 
CCTGCTGCCT CCTTGCTGGG TGGCCACCCG CGACTAGAGA CTGCTCATGC TAGCAGCTTA 
AGCCATTTGG CATTAGCACA CCAGCAGCAG CAACAGATGT TACAACACCA GTCTCCACAT 
CTTCTTGGAC AAGCTCATCC TTCTGCTTCC TATAATCAGC TAGGGCTTTA TCCAATTATT 
TGGCAGTATC CAAATGGAAC ACATGCTTAC TCAGGACTCG GTCTGCCCTC CTCCAAATGG 
GTCCACCCAG AAACTCCTGT TAACGCGGAG GCTTCCTTGA GAAGGAATAC TCCCAGCCCA 
TGGTTACACC AGCCCACCCC TGTGACCTCA GCTGACAGCC TCGGTTTACT GAGTCACATT 
CCTGTGCGAC CGTCCAGTGC AGATCCCCTT CGGCCTCTCA AACTGACAAC GCATTCCAGC 
CCACCATTGT CCAAAAGTAT CATAGAGCAT CACAAAGAAG AACTGGAGAG GAAAGCGTTT 
ATTGAACCTT TACGTTCTGT TACAACTGCG CCAGTAAAGA CGGAGCTAGA GCAGAGCAGA 
ACACAGACGG CGAAGGAGAG CCATATGCAC AGACATTTTG CAGATCCAAT GTTGAACCAG 
CTGCCAAGGC CACCACAGGA GACTGGGGAG CGACTGAGCA AATACAAAGA AGAACACCGA 
CGGATACTCC AAGAAAGTAT TGAAGTTGCT CCTTTTACAG CTAAAATAAA GGCACTGGAG 
GGAGAGAGAG AGAACTATTC CAGGGTTGCG TCCTTATCTT CAAGTCCCAA AAGCCATTCG 
GTAAAATATG ACAAAGATGC CGAACGCTCT GTGTCAGAGC TGTACAAAAT GAAGCATTCG 
GTTCCACAGA GTTTACCACA AAGTAACTAC TTCACCACCT TGTCTAACAG TGTAGTAAAT 
GAACCGCCGA GATCCTACCC ATCAAAGGAG ACTTCAAGCG CGTATGTTGA TAAGCAGACT 
AATTGTGCTT CAACAGCAGC TAGTCCCCAG TCTCTCCCCT CTTACATTTC TTCTCTTTCA 
AAACCTCCAC CTTTAATTAA GCACCAACCA GAGAGCGAAG GCTCTGCGAG CAAGATACCT 
GAGCAGCTCT CGCAGTCAGT GCAGTCTCAC TCTGTAAATT CTTTTAGAAG CGACAGCAGG 
AGCCCTACTC AGTTGACAGT CTCCTCTTCA AATACACTCC GGAGTATGCC TGCCTTGCAT 
AGAGCCCCTG TGTTTCACCC CCCTGTGCAC CAGAACCTGG AGAAGAAGGA AAGCAGCTAT 
AGCAGCCTTT CACCTCCAAC TCTGACTCCC GTTCAGCCTG TTAATGCTGG TGGTGGCAAA 
ATACAGGAGT TGCAGAAACC ACCAACTCTA GTACCTGAGC CTAAGGAAGC TCAGACTGTT 
TACAAGGGCA CTTCTGAACA GAATGTATCA GAGGCATGGA AGTCCAATAG TGCCCCAAAT 
AACGAAAAAG TGGATTGGCA CGTTGAAAGA ATGAGTGGAA AGTCACAGTC CGCTACAGCA 
TCTGTTATTG TGCGTCCTCC TTCTAGCACG AAATACGATA GCGTAGCAGT AATGCAGTCT 
GCTTCCAAAG ACCGAGGCAG TGAGAGATCT TCAGCTGTGA CAAATCAAGC AGATTGCCTG 
AAAACAGCGG AAGCCAGGGA GACTGGAAGA ATCATTCTGC CAAATATGAA CTCAGACAGC 
GCTCGCACAC AGTATGAAAA GAAATTTCCA GCTGTCTCGC AAGGCAGCAT TCCGTGTGCT 
GTCACCCCTA CCACAACGAT GATCTGCAGT ACCAAAACGG ATGTCACCGC ATCGGCAGCA 
ACAACTACCA GCGTGTCGAG CTGGGGTAGT TCAGAGGTGA CTTACTCCTT ATCGAACACG 
GTCCTGGCCT GCGCGCCGCT GGAGTGTACG GCCTCGAGAG CCGCAAGTCA GGCGGTGGCA 
CAGGCCCAGG AATGCAAGGT CAGCACTCCG GCTCCGGTTA CACCCGCCGC TGGCAAGGCA 
GGCGGTGGTG CTCAGCCCAG CTCGGGATTC TCCACCTCTA CTGACTTTGT CCATTTGAAA 
AAGCACAAGG CGGCCTTGGC TGCAGCTCAG TTTAAAAGTA GTAACACCAG TGAGACCGAG 
TCCAACTCTG TGAAAAATCA GACATTTTCA ACCTCTCTCT CCCTAGACAG CACTATCGTC 
TGTAATACAA TAAACAAAGC GAACTCTGTA GGCAGTGGGC AAACTTCCCA GACGAGTCAG 
CCAAACTACC ACACTAAACT GAAAAAGGCT TGGCTAACAA GACATTCGGA GGAAGATAAA 
AACACTAATA AAAAAGAGAA TTCAGGGAAC AGTGTCTCAG AAATTATTAA GCCATGTACT 
GTCAATTTAA TAGCTTCTAC ATCAAATGAT TTGCAAAATA ACATAGACGG TAAAATCTTG 
GCAGATAAGT TTGCGAAGGA AGATAAGCAC CCTCGGAGAA AAGCAAAACG AACTTACGAG 
TCTGGCTCTG AAAGTGGAGA CTCTGATGAA AGCGAGAGCA AGTCAGAGCA AAGGACTAAA 
CGGCAGCCCA AGCCAACTTA CAAAAAGAAA CAAAATGATT TGCAGAAAAA AAAGGGTGAT 
ACGGAGGAAG AAGTAAAACC GAATGGTGTT CTTAGCAGGA GTGCCAAAGA GAAAAGCAAG 
CTGAAGTTAC AGAGCGGCAG TAACAGCACT GGTATACCTC GCTCAGTGTT AAAAGACTGG 
CGCAAAGTAA AGAAGCTGAA GCAAACAGGA GAGTCCTTTT TGCAGGATGA TTCTTGCTCT 
GAAATAGGAC CAAATTTGCA AAAATGCAGA GAATGTAGGT TAATAAGAAG TAAGAAAGGA 
GAAGAACCAA CTCACTCACC AGTATTTTGT AGATTTTACT ACTTTCGGCG GTTATCTTTC 
AGTAAGAATG GAGTGGTTAG GATAGATGGT TTCTCCTCTC CTGATCAATA TGATGATGAA 
GCACTAAGTT TATGGACGCA TGAAAACTAT GAAGATGATG AACTGGACCT AGAAACTTCT 
AAATACATTC TAGATATCAT AGGTGATAAA TTTTGTCAGT TGGTAACGTC TGAGAAAACA 
GCCATGTCCT GGGTGAAGAA AGACGCCAAA ATTGCATGGA AGAGAGCAGT GAGAGGAGTC 
CGTGAGATGT GTGATGCATG TGAAGCCACA TTATTTAACA TTCATTGGGT CTGCCAAAAA 
TGTGGATTTG TGGTCTGCCT AGATTGTTAC AAAGCAAAGG AAAGAAAAAG TTCTAGAGAT 
AAAGAGTTAT ATGCCTGGAT GAAGTGTGTG AAGGGACAGC CTCACGATCA CAAACACCTG 
ATGCCAACAC AGATTATTCC AGGCTCTGTT TTGACAGACC TTCTAGATGC TATGCACAGT 
ATTAGAGAAA AATTTGAAAT TAAATCCCAT TGTCAGTGTA CCAGCAAGCA GAGCACACAA 
GCTGGCAAGC TCCCTGCAAT GAATGGTGTG TCTCAGGTTT TGCAGAATGT CCTCAACCAC 
AGTAATAAAA TTTCTCTGTG CATGCCTGAG TCTCAACAGC AGAATACTCC TCAAAAGTCT 
GAGACAAATG GTAATACAAG TCCTAGAAGC GATGTAAGCA CAGACAGCAA GTTAACACCT 
CCTGAATCCC AGTCACCGCT GCACTGGTTA GCTGATCTTG CAGAGCAGAA AGCCAGAGAA 
GAAAAGAAAG AGAACAAGGA ATGTCCTTCT GGAAAACATT CAAAGGAAGA TAAAGACCAG 
GATAATTTGG AGTCCCAGAA CTGTAAAAGC TCCCCTCCTG CCTCCCAGAA CAACGAGCAG 
GGCTCAACCT TACGAGATTT ATTAACTACA ACAGCAGGCA AACTGCGCCT GGGTTCTACA 
GATGCTGGGA TTGCTTTTGC TCCAGTTTAC TCTACAGGAA CAGCAAGTGG CAAGAGTGGA 
AGGACTATGC CAAACATTCT TGATGATATA ATTGCTTCAG TAGTAGAAAA CAAGATTCCA 
CCAAATAGAG CACCAAAGAT AAATGTAAAA TCTGAAATAA AAGACGAGCC AAAGGACGAC 
AAAAAATGTG TTCAGGATGA CTGCAGTAAA CTGTACAGTG ATATTCAGTA TTCATGGATC 
TGTGATAAGC ATGTTTTGTG GCTCAGAGAC CATAAAAACA ACAACAACGG GAAGCTCTTC 
AAGGAGTGTT GGAGACAAGG AAGGCCTGTT CTAGTGTCCG GTATGCATAA GAAAATGAAC 
TTCAGCCTGT GGAAAGCTGA GTCAATTAGT CTAGACTTTG GAAACCAGCA AGCTGATATC 
TTGAATTGCA AAGACAGTAT TATTTCAAAC ACCAATGTCA AGGAGTTCTG GGATGGTTTT 
GAAGATGTTT CAAAACGGCA GAAAATAAAA AATGGGGAAA CAGCTCTACT GAAACTGAAA 
GACTGGCCTT CTGGTGAAGA TTTCAAGGCT ATGATGCCAG CAAGGTATGA GGACTTATTA 
AAAAGTTTAC CCTTGCCTGA ATACTGTAGT CCAGAAGGAA AGCTAAACCT GGCTTCTCAT 
CTGCCGGGAT TTTTTGTCCG TCCGGATTTG GGACCCAGAC TGTGCAGTGC CTATGGTGTG 
GCTGCGACTA AAGACCATGA TATAGGAACC ACAAATCTCC ATATTGAAGT TTCTGATGTT 
GTGAACATCC TTGTTTACGT TGGCATAGCA AAAGGAAATG GAGTGCTTTC CAAATCAGGA 
GTTTTGAAGA AGTTTGAAGA GGAAGATTTG GATGACCTTT TAAGGAAGCG GTTGAAAGAT 
TCAAGTGAAT TACCTGGTGC TTTATGGCAC ATTTATGCTG GCAAAGACGC TGACAAGATA 
AGGGAGTTTC TGCAAAAGAT AGCAAAAGAG CAAGGCTTAG AAGTTTTACC GGAGCATGAT 
CCGATACGTG ATCAGAGTTG GTACGTGAAC AAAAAACTTC GCCAAAGACT TCTTGAAGAA 
TATGGAGTGA AAACCTGCAC GGTTATTCAG TTTCTTGGTG ATGCTATTAT TCTACCAGCA 
GGAGCACTTC ATCAGGTGCA AAATTTTCAC AGCTGCGTTC AAGTAACTGA AGACTTTGTG 
TCTCCAGAAC ATCTTGTACA GTCCTTTCAC CTAACGCAGG AGCTGCGGCT GTCAAAGGAA 
GAAATCAATT ATGATGATAA ACTGCAGGTT AAAAATATCT TGTATCATGC AGTTAAAGAA 
ATGGTGAGAG CTCTGAAGAT CCATGAAGGT GAAATGGAAG ATATGGATGA AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JMJD1C ( XM_014940495.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014795981.1 Calidris pugnax jumonji domain containing 1C (JMJD1C), transcript variant X2, mRNA.

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CloneID	OCa63958
Clone ID Related Accession (Same CDS sequence)	XM_014940495.1
Accession Version	XM_014940495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable JmjC domain-containing histone demethylation protein 2C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090828.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTTA TGAATGACCA GGTGTTAGAA CCTCAGAATG TTGATCCTTC TATGGTTCAG 
ATGACCTTTC TAGATGATGT TGTTCACTCT TTGTTGAAAG GTGAAAATAT TGGCATCACT 
TCACGCCGAC GGTCTCGTTC CAACCAGAAC AGCAACACAG TCCACGGTCA TTATACACGT 
GCGCAAGCAA ATAGTCCCAG ACCAGCAATG AATTCCCAAA CTGCAGCACC AAAACAAAAT 
TCTCACCAGC ACCAGCAACA GAGGAATACT CGGCCGAATA AGAGGAAAGG CTCTGATAGC 
AGCATGCCTG ATGAAGAGAA GATGAAAGAA GAAAGATACG ATTATATAGG TCGAGGAGAA 
AACCCCAAAA ACAAAAATAA ACACTTCCTT AGTAAAAGAA GAAAACCTGA AGAGGATGAA 
AAAAAGTTAA ATATGAAGAG ACTGCGGACA GACAATATCT CAGATTACTC TGAGAGCAGT 
GACTCAGAAA TTTCAAATAA AAGATTAACA GATTCGTCCT CAGAGCAAAA TTCGGAAAAT 
GAGTTAAAAA GCAAGAACAC TTCAAAGATA AATGGAGAAG AAGGAAAATC CCAAACTGTT 
GAGGGAGTAG AACAGACACT AACAGATAGG CGGTCTCCAT GGGATGAAAT ACAGGAGGAT 
AAAAAACACG AAGAGACAGA AAGACTTAAG TCAGCCGTCT TAGATCAGCA GGAAAAATCT 
TCACTCCATG CAGCAGAGCA GCCAACACCT TATGAGCAGA ATGCCAAGGA TCCACATGTT 
CAAGAGTGCA ATGCAGAAAA ACATCATACT GCGGAATTAA AAAATGAGAA GTTTGTACCC 
GGACCTCCTA CTCCGAAATG TGTTGTTGAC ATTACTAATG AAAACAACTC TGAAAAGGAG 
AGCCAGGATA ATGCTGCAAG TACCTTTGGT TTGCAAACGG TTCAGAAAGG GGAATCTCAC 
AGCAGCGATT TAAAACAGCA GTTTGCAAAT GCAAATTTCC TTGAAACAAG AAAACAGGAA 
GCTGATCAAA GTTGGGTTAG CAGTGTCGTT AGCAAAACGG ATTTCATACA ACCTGGAGTT 
GTAAAAACAT TATCAGTAAA TGAACACTTA AATCCTGAAA AAGAAAAAGT GCAGTATGGC 
TCCTTTATGT CTTCGTTAAA TGTAGCTTCT CTAGCAGAAG AGAGTAAACT TCATAAACAA 
AGTCCAGTCC CCGATTCTGT GAAGTCAAAA CCTAGTGCTT CAGTTGATCA TCCTAAAACA 
AAGTCACCTG ATGTTAAACC TAAATTCACC CAATCTTCTG ATACTGTGAA GTCTAAGGTT 
AGTTCCCAAA ACAGCCATGC TACTGGATTA ACAAGGTCAG CCAATAAAAC TGATCATGAT 
TTGCCTAGGT CTAGTTTTCA TCCAGTTCCA GCTAGAGTTA GCGCTCTAGA AGCTACTAAA 
AGTCCTCTTA TCATTGACAA GAATGAACAT TTCACGGTGT ACAGGGACCC CACTCTGATT 
GGACAAGAAA CAGGAACTAA TCACATTTCA CCTTATTTGC ATCAGCATAA TTATCCTCTG 
CATTCCTCAT CCCACCGAAC CTGTTTAAAT CCAAACGCTC ATCATCCTGC ATTAACTGGT 
TCATCCCATC TGCTAGCTGG GTCTTCAGCT CAGGCTCCCC TGTCCGCTAT TAACACTCAC 
CCCCTTAGTA CCGCATCCCA CCATTCTGTT CATCACCCTC ATCTACTTCC TGCAGTGTTA 
CCCGGAGTGC CTGCTGCCTC CTTGCTGGGT GGCCACCCGC GACTAGAGAC TGCTCATGCT 
AGCAGCTTAA GCCATTTGGC ATTAGCACAC CAGCAGCAGC AACAGATGTT ACAACACCAG 
TCTCCACATC TTCTTGGACA AGCTCATCCT TCTGCTTCCT ATAATCAGCT AGGGCTTTAT 
CCAATTATTT GGCAGTATCC AAATGGAACA CATGCTTACT CAGGACTCGG TCTGCCCTCC 
TCCAAATGGG TCCACCCAGA AACTCCTGTT AACGCGGAGG CTTCCTTGAG AAGGAATACT 
CCCAGCCCAT GGTTACACCA GCCCACCCCT GTGACCTCAG CTGACAGCCT CGGTTTACTG 
AGTCACATTC CTGTGCGACC GTCCAGTGCA GATCCCCTTC GGCCTCTCAA ACTGACAACG 
CATTCCAGCC CACCATTGTC CAAAAGTATC ATAGAGCATC ACAAAGAAGA ACTGGAGAGG 
AAAGCGTTTA TTGAACCTTT ACGTTCTGTT ACAACTGCGC CAGTAAAGAC GGAGCTAGAG 
CAGAGCAGAA CACAGACGGC GAAGGAGAGC CATATGCACA GACATTTTGC AGATCCAATG 
TTGAACCAGC TGCCAAGGCC ACCACAGGAG ACTGGGGAGC GACTGAGCAA ATACAAAGAA 
GAACACCGAC GGATACTCCA AGAAAGTATT GAAGTTGCTC CTTTTACAGC TAAAATAAAG 
GCACTGGAGG GAGAGAGAGA GAACTATTCC AGGGTTGCGT CCTTATCTTC AAGTCCCAAA 
AGCCATTCGG TAAAATATGA CAAAGATGCC GAACGCTCTG TGTCAGAGCT GTACAAAATG 
AAGCATTCGG TTCCACAGAG TTTACCACAA AGTAACTACT TCACCACCTT GTCTAACAGT 
GTAGTAAATG AACCGCCGAG ATCCTACCCA TCAAAGGAGA CTTCAAGCGC GTATGTTGAT 
AAGCAGACTA ATTGTGCTTC AACAGCAGCT AGTCCCCAGT CTCTCCCCTC TTACATTTCT 
TCTCTTTCAA AACCTCCACC TTTAATTAAG CACCAACCAG AGAGCGAAGG CTCTGCGAGC 
AAGATACCTG AGCAGCTCTC GCAGTCAGTG CAGTCTCACT CTGTAAATTC TTTTAGAAGC 
GACAGCAGGA GCCCTACTCA GTTGACAGTC TCCTCTTCAA ATACACTCCG GAGTATGCCT 
GCCTTGCATA GAGCCCCTGT GTTTCACCCC CCTGTGCACC AGAACCTGGA GAAGAAGGAA 
AGCAGCTATA GCAGCCTTTC ACCTCCAACT CTGACTCCCG TTCAGCCTGT TAATGCTGGT 
GGTGGCAAAA TACAGGAGTT GCAGAAACCA CCAACTCTAG TACCTGAGCC TAAGGAAGCT 
CAGACTGTTT ACAAGGGCAC TTCTGAACAG AATGTATCAG AGGCATGGAA GTCCAATAGT 
GCCCCAAATA ACGAAAAAGT GGATTGGCAC GTTGAAAGAA TGAGTGGAAA GTCACAGTCC 
GCTACAGCAT CTGTTATTGT GCGTCCTCCT TCTAGCACGA AATACGATAG CGTAGCAGTA 
ATGCAGTCTG CTTCCAAAGA CCGAGGCAGT GAGAGATCTT CAGCTGTGAC AAATCAAGCA 
GATTGCCTGA AAACAGCGGA AGCCAGGGAG ACTGGAAGAA TCATTCTGCC AAATATGAAC 
TCAGACAGCG CTCGCACACA GTATGAAAAG AAATTTCCAG CTGTCTCGCA AGGCAGCATT 
CCGTGTGCTG TCACCCCTAC CACAACGATG ATCTGCAGTA CCAAAACGGA TGTCACCGCA 
TCGGCAGCAA CAACTACCAG CGTGTCGAGC TGGGGTAGTT CAGAGGTGAC TTACTCCTTA 
TCGAACACGG TCCTGGCCTG CGCGCCGCTG GAGTGTACGG CCTCGAGAGC CGCAAGTCAG 
GCGGTGGCAC AGGCCCAGGA ATGCAAGGTC AGCACTCCGG CTCCGGTTAC ACCCGCCGCT 
GGCAAGGCAG GCGGTGGTGC TCAGCCCAGC TCGGGATTCT CCACCTCTAC TGACTTTGTC 
CATTTGAAAA AGCACAAGGC GGCCTTGGCT GCAGCTCAGT TTAAAAGTAG TAACACCAGT 
GAGACCGAGT CCAACTCTGT GAAAAATCAG ACATTTTCAA CCTCTCTCTC CCTAGACAGC 
ACTATCGTCT GTAATACAAT AAACAAAGCG AACTCTGTAG GCAGTGGGCA AACTTCCCAG 
ACGAGTCAGC CAAACTACCA CACTAAACTG AAAAAGGCTT GGCTAACAAG ACATTCGGAG 
GAAGATAAAA ACACTAATAA AAAAGAGAAT TCAGGGAACA GTGTCTCAGA AATTATTAAG 
CCATGTACTG TCAATTTAAT AGCTTCTACA TCAAATGATT TGCAAAATAA CATAGACGGT 
AAAATCTTGG CAGATAAGTT TGCGAAGGAA GATAAGCACC CTCGGAGAAA AGCAAAACGA 
ACTTACGAGT CTGGCTCTGA AAGTGGAGAC TCTGATGAAA GCGAGAGCAA GTCAGAGCAA 
AGGACTAAAC GGCAGCCCAA GCCAACTTAC AAAAAGAAAC AAAATGATTT GCAGAAAAAA 
AAGGGTGATA CGGAGGAAGA AGTAAAACCG AATGGTGTTC TTAGCAGGAG TGCCAAAGAG 
AAAAGCAAGC TGAAGTTACA GAGCGGCAGT AACAGCACTG GTATACCTCG CTCAGTGTTA 
AAAGACTGGC GCAAAGTAAA GAAGCTGAAG CAAACAGGAG AGTCCTTTTT GCAGGATGAT 
TCTTGCTCTG AAATAGGACC AAATTTGCAA AAATGCAGAG AATGTAGGTT AATAAGAAGT 
AAGAAAGGAG AAGAACCAAC TCACTCACCA GTATTTTGTA GATTTTACTA CTTTCGGCGG 
TTATCTTTCA GTAAGAATGG AGTGGTTAGG ATAGATGGTT TCTCCTCTCC TGATCAATAT 
GATGATGAAG CACTAAGTTT ATGGACGCAT GAAAACTATG AAGATGATGA ACTGGACCTA 
GAAACTTCTA AATACATTCT AGATATCATA GGTGATAAAT TTTGTCAGTT GGTAACGTCT 
GAGAAAACAG CCATGTCCTG GGTGAAGAAA GACGCCAAAA TTGCATGGAA GAGAGCAGTG 
AGAGGAGTCC GTGAGATGTG TGATGCATGT GAAGCCACAT TATTTAACAT TCATTGGGTC 
TGCCAAAAAT GTGGATTTGT GGTCTGCCTA GATTGTTACA AAGCAAAGGA AAGAAAAAGT 
TCTAGAGATA AAGAGTTATA TGCCTGGATG AAGTGTGTGA AGGGACAGCC TCACGATCAC 
AAACACCTGA TGCCAACACA GATTATTCCA GGCTCTGTTT TGACAGACCT TCTAGATGCT 
ATGCACAGTA TTAGAGAAAA ATTTGAAATT AAATCCCATT GTCAGTGTAC CAGCAAGCAG 
AGCACACAAG CTGGCAAGCT CCCTGCAATG AATGGTGTGT CTCAGGTTTT GCAGAATGTC 
CTCAACCACA GTAATAAAAT TTCTCTGTGC ATGCCTGAGT CTCAACAGCA GAATACTCCT 
CAAAAGTCTG AGACAAATGG TAATACAAGT CCTAGAAGCG ATGTAAGCAC AGACAGCAAG 
TTAACACCTC CTGAATCCCA GTCACCGCTG CACTGGTTAG CTGATCTTGC AGAGCAGAAA 
GCCAGAGAAG AAAAGAAAGA GAACAAGGAA TGTCCTTCTG GAAAACATTC AAAGGAAGAT 
AAAGACCAGG ATAATTTGGA GTCCCAGAAC TGTAAAAGCT CCCCTCCTGC CTCCCAGAAC 
AACGAGCAGG GCTCAACCTT ACGAGATTTA TTAACTACAA CAGCAGGCAA ACTGCGCCTG 
GGTTCTACAG ATGCTGGGAT TGCTTTTGCT CCAGTTTACT CTACAGGAAC AGCAAGTGGC 
AAGAGTGGAA GGACTATGCC AAACATTCTT GATGATATAA TTGCTTCAGT AGTAGAAAAC 
AAGATTCCAC CAAATAGAGC ACCAAAGATA AATGTAAAAT CTGAAATAAA AGACGAGCCA 
AAGGACGACA AAAAATGTGT TCAGGATGAC TGCAGTAAAC TGTACAGTGA TATTCAGTAT 
TCATGGATCT GTGATAAGCA TGTTTTGTGG CTCAGAGACC ATAAAAACAA CAACAACGGG 
AAGCTCTTCA AGGAGTGTTG GAGACAAGGA AGGCCTGTTC TAGTGTCCGG TATGCATAAG 
AAAATGAACT TCAGCCTGTG GAAAGCTGAG TCAATTAGTC TAGACTTTGG AAACCAGCAA 
GCTGATATCT TGAATTGCAA AGACAGTATT ATTTCAAACA CCAATGTCAA GGAGTTCTGG 
GATGGTTTTG AAGATGTTTC AAAACGGCAG AAAATAAAAA ATGGGGAAAC AGCTCTACTG 
AAACTGAAAG ACTGGCCTTC TGGTGAAGAT TTCAAGGCTA TGATGCCAGC AAGGTATGAG 
GACTTATTAA AAAGTTTACC CTTGCCTGAA TACTGTAGTC CAGAAGGAAA GCTAAACCTG 
GCTTCTCATC TGCCGGGATT TTTTGTCCGT CCGGATTTGG GACCCAGACT GTGCAGTGCC 
TATGGTGTGG CTGCGACTAA AGACCATGAT ATAGGAACCA CAAATCTCCA TATTGAAGTT 
TCTGATGTTG TGAACATCCT TGTTTACGTT GGCATAGCAA AAGGAAATGG AGTGCTTTCC 
AAATCAGGAG TTTTGAAGAA GTTTGAAGAG GAAGATTTGG ATGACCTTTT AAGGAAGCGG 
TTGAAAGATT CAAGTGAATT ACCTGGTGCT TTATGGCACA TTTATGCTGG CAAAGACGCT 
GACAAGATAA GGGAGTTTCT GCAAAAGATA GCAAAAGAGC AAGGCTTAGA AGTTTTACCG 
GAGCATGATC CGATACGTGA TCAGAGTTGG TACGTGAACA AAAAACTTCG CCAAAGACTT 
CTTGAAGAAT ATGGAGTGAA AACCTGCACG GTTATTCAGT TTCTTGGTGA TGCTATTATT 
CTACCAGCAG GAGCACTTCA TCAGGTGCAA AATTTTCACA GCTGCGTTCA AGTAACTGAA 
GACTTTGTGT CTCCAGAACA TCTTGTACAG TCCTTTCACC TAACGCAGGA GCTGCGGCTG 
TCAAAGGAAG AAATCAATTA TGATGATAAA CTGCAGGTTA AAAATATCTT GTATCATGCA 
GTTAAAGAAA TGGTGAGAGC TCTGAAGATC CATGAAGGTG AAATGGAAGA TATGGATGAA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014795981.1
CDS	219..7184
Translation	MVVMNDQVLEPQNVDPSMVQMTFLDDVVHSLLKGENIGITSRRRSRSNQNSNTVHGHYTRAQANSPRPAMNSQTAAPKQNSHQHQQQRNTRPNKRKGSDSSMPDEEKMKEERYDYIGRGENPKNKNKHFLSKRRKPEEDEKKLNMKRLRTDNISDYSESSDSEISNKRLTDSSSEQNSENELKSKNTSKINGEEGKSQTVEGVEQTLTDRRSPWDEIQEDKKHEETERLKSAVLDQQEKSSLHAAEQPTPYEQNAKDPHVQECNAEKHHTAELKNEKFVPGPPTPKCVVDITNENNSEKESQDNAASTFGLQTVQKGESHSSDLKQQFANANFLETRKQEADQSWVSSVVSKTDFIQPGVVKTLSVNEHLNPEKEKVQYGSFMSSLNVASLAEESKLHKQSPVPDSVKSKPSASVDHPKTKSPDVKPKFTQSSDTVKSKVSSQNSHATGLTRSANKTDHDLPRSSFHPVPARVSALEATKSPLIIDKNEHFTVYRDPTLIGQETGTNHISPYLHQHNYPLHSSSHRTCLNPNAHHPALTGSSHLLAGSSAQAPLSAINTHPLSTASHHSVHHPHLLPAVLPGVPAASLLGGHPRLETAHASSLSHLALAHQQQQQMLQHQSPHLLGQAHPSASYNQLGLYPIIWQYPNGTHAYSGLGLPSSKWVHPETPVNAEASLRRNTPSPWLHQPTPVTSADSLGLLSHIPVRPSSADPLRPLKLTTHSSPPLSKSIIEHHKEELERKAFIEPLRSVTTAPVKTELEQSRTQTAKESHMHRHFADPMLNQLPRPPQETGERLSKYKEEHRRILQESIEVAPFTAKIKALEGERENYSRVASLSSSPKSHSVKYDKDAERSVSELYKMKHSVPQSLPQSNYFTTLSNSVVNEPPRSYPSKETSSAYVDKQTNCASTAASPQSLPSYISSLSKPPPLIKHQPESEGSASKIPEQLSQSVQSHSVNSFRSDSRSPTQLTVSSSNTLRSMPALHRAPVFHPPVHQNLEKKESSYSSLSPPTLTPVQPVNAGGGKIQELQKPPTLVPEPKEAQTVYKGTSEQNVSEAWKSNSAPNNEKVDWHVERMSGKSQSATASVIVRPPSSTKYDSVAVMQSASKDRGSERSSAVTNQADCLKTAEARETGRIILPNMNSDSARTQYEKKFPAVSQGSIPCAVTPTTTMICSTKTDVTASAATTTSVSSWGSSEVTYSLSNTVLACAPLECTASRAASQAVAQAQECKVSTPAPVTPAAGKAGGGAQPSSGFSTSTDFVHLKKHKAALAAAQFKSSNTSETESNSVKNQTFSTSLSLDSTIVCNTINKANSVGSGQTSQTSQPNYHTKLKKAWLTRHSEEDKNTNKKENSGNSVSEIIKPCTVNLIASTSNDLQNNIDGKILADKFAKEDKHPRRKAKRTYESGSESGDSDESESKSEQRTKRQPKPTYKKKQNDLQKKKGDTEEEVKPNGVLSRSAKEKSKLKLQSGSNSTGIPRSVLKDWRKVKKLKQTGESFLQDDSCSEIGPNLQKCRECRLIRSKKGEEPTHSPVFCRFYYFRRLSFSKNGVVRIDGFSSPDQYDDEALSLWTHENYEDDELDLETSKYILDIIGDKFCQLVTSEKTAMSWVKKDAKIAWKRAVRGVREMCDACEATLFNIHWVCQKCGFVVCLDCYKAKERKSSRDKELYAWMKCVKGQPHDHKHLMPTQIIPGSVLTDLLDAMHSIREKFEIKSHCQCTSKQSTQAGKLPAMNGVSQVLQNVLNHSNKISLCMPESQQQNTPQKSETNGNTSPRSDVSTDSKLTPPESQSPLHWLADLAEQKAREEKKENKECPSGKHSKEDKDQDNLESQNCKSSPPASQNNEQGSTLRDLLTTTAGKLRLGSTDAGIAFAPVYSTGTASGKSGRTMPNILDDIIASVVENKIPPNRAPKINVKSEIKDEPKDDKKCVQDDCSKLYSDIQYSWICDKHVLWLRDHKNNNNGKLFKECWRQGRPVLVSGMHKKMNFSLWKAESISLDFGNQQADILNCKDSIISNTNVKEFWDGFEDVSKRQKIKNGETALLKLKDWPSGEDFKAMMPARYEDLLKSLPLPEYCSPEGKLNLASHLPGFFVRPDLGPRLCSAYGVAATKDHDIGTTNLHIEVSDVVNILVYVGIAKGNGVLSKSGVLKKFEEEDLDDLLRKRLKDSSELPGALWHIYAGKDADKIREFLQKIAKEQGLEVLPEHDPIRDQSWYVNKKLRQRLLEEYGVKTCTVIQFLGDAIILPAGALHQVQNFHSCVQVTEDFVSPEHLVQSFHLTQELRLSKEEINYDDKLQVKNILYHAVKEMVRALKIHEGEMEDMDEN

Target ORF information:

RefSeq Version	XM_014940495.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax jumonji domain containing 1C (JMJD1C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014940495.1

ORF Insert Sequence:

ATGGTTGTTA TGAATGACCA GGTGTTAGAA CCTCAGAATG TTGATCCTTC TATGGTTCAG 
ATGACCTTTC TAGATGATGT TGTTCACTCT TTGTTGAAAG GTGAAAATAT TGGCATCACT 
TCACGCCGAC GGTCTCGTTC CAACCAGAAC AGCAACACAG TCCACGGTCA TTATACACGT 
GCGCAAGCAA ATAGTCCCAG ACCAGCAATG AATTCCCAAA CTGCAGCACC AAAACAAAAT 
TCTCACCAGC ACCAGCAACA GAGGAATACT CGGCCGAATA AGAGGAAAGG CTCTGATAGC 
AGCATGCCTG ATGAAGAGAA GATGAAAGAA GAAAGATACG ATTATATAGG TCGAGGAGAA 
AACCCCAAAA ACAAAAATAA ACACTTCCTT AGTAAAAGAA GAAAACCTGA AGAGGATGAA 
AAAAAGTTAA ATATGAAGAG ACTGCGGACA GACAATATCT CAGATTACTC TGAGAGCAGT 
GACTCAGAAA TTTCAAATAA AAGATTAACA GATTCGTCCT CAGAGCAAAA TTCGGAAAAT 
GAGTTAAAAA GCAAGAACAC TTCAAAGATA AATGGAGAAG AAGGAAAATC CCAAACTGTT 
GAGGGAGTAG AACAGACACT AACAGATAGG CGGTCTCCAT GGGATGAAAT ACAGGAGGAT 
AAAAAACACG AAGAGACAGA AAGACTTAAG TCAGCCGTCT TAGATCAGCA GGAAAAATCT 
TCACTCCATG CAGCAGAGCA GCCAACACCT TATGAGCAGA ATGCCAAGGA TCCACATGTT 
CAAGAGTGCA ATGCAGAAAA ACATCATACT GCGGAATTAA AAAATGAGAA GTTTGTACCC 
GGACCTCCTA CTCCGAAATG TGTTGTTGAC ATTACTAATG AAAACAACTC TGAAAAGGAG 
AGCCAGGATA ATGCTGCAAG TACCTTTGGT TTGCAAACGG TTCAGAAAGG GGAATCTCAC 
AGCAGCGATT TAAAACAGCA GTTTGCAAAT GCAAATTTCC TTGAAACAAG AAAACAGGAA 
GCTGATCAAA GTTGGGTTAG CAGTGTCGTT AGCAAAACGG ATTTCATACA ACCTGGAGTT 
GTAAAAACAT TATCAGTAAA TGAACACTTA AATCCTGAAA AAGAAAAAGT GCAGTATGGC 
TCCTTTATGT CTTCGTTAAA TGTAGCTTCT CTAGCAGAAG AGAGTAAACT TCATAAACAA 
AGTCCAGTCC CCGATTCTGT GAAGTCAAAA CCTAGTGCTT CAGTTGATCA TCCTAAAACA 
AAGTCACCTG ATGTTAAACC TAAATTCACC CAATCTTCTG ATACTGTGAA GTCTAAGGTT 
AGTTCCCAAA ACAGCCATGC TACTGGATTA ACAAGGTCAG CCAATAAAAC TGATCATGAT 
TTGCCTAGGT CTAGTTTTCA TCCAGTTCCA GCTAGAGTTA GCGCTCTAGA AGCTACTAAA 
AGTCCTCTTA TCATTGACAA GAATGAACAT TTCACGGTGT ACAGGGACCC CACTCTGATT 
GGACAAGAAA CAGGAACTAA TCACATTTCA CCTTATTTGC ATCAGCATAA TTATCCTCTG 
CATTCCTCAT CCCACCGAAC CTGTTTAAAT CCAAACGCTC ATCATCCTGC ATTAACTGGT 
TCATCCCATC TGCTAGCTGG GTCTTCAGCT CAGGCTCCCC TGTCCGCTAT TAACACTCAC 
CCCCTTAGTA CCGCATCCCA CCATTCTGTT CATCACCCTC ATCTACTTCC TGCAGTGTTA 
CCCGGAGTGC CTGCTGCCTC CTTGCTGGGT GGCCACCCGC GACTAGAGAC TGCTCATGCT 
AGCAGCTTAA GCCATTTGGC ATTAGCACAC CAGCAGCAGC AACAGATGTT ACAACACCAG 
TCTCCACATC TTCTTGGACA AGCTCATCCT TCTGCTTCCT ATAATCAGCT AGGGCTTTAT 
CCAATTATTT GGCAGTATCC AAATGGAACA CATGCTTACT CAGGACTCGG TCTGCCCTCC 
TCCAAATGGG TCCACCCAGA AACTCCTGTT AACGCGGAGG CTTCCTTGAG AAGGAATACT 
CCCAGCCCAT GGTTACACCA GCCCACCCCT GTGACCTCAG CTGACAGCCT CGGTTTACTG 
AGTCACATTC CTGTGCGACC GTCCAGTGCA GATCCCCTTC GGCCTCTCAA ACTGACAACG 
CATTCCAGCC CACCATTGTC CAAAAGTATC ATAGAGCATC ACAAAGAAGA ACTGGAGAGG 
AAAGCGTTTA TTGAACCTTT ACGTTCTGTT ACAACTGCGC CAGTAAAGAC GGAGCTAGAG 
CAGAGCAGAA CACAGACGGC GAAGGAGAGC CATATGCACA GACATTTTGC AGATCCAATG 
TTGAACCAGC TGCCAAGGCC ACCACAGGAG ACTGGGGAGC GACTGAGCAA ATACAAAGAA 
GAACACCGAC GGATACTCCA AGAAAGTATT GAAGTTGCTC CTTTTACAGC TAAAATAAAG 
GCACTGGAGG GAGAGAGAGA GAACTATTCC AGGGTTGCGT CCTTATCTTC AAGTCCCAAA 
AGCCATTCGG TAAAATATGA CAAAGATGCC GAACGCTCTG TGTCAGAGCT GTACAAAATG 
AAGCATTCGG TTCCACAGAG TTTACCACAA AGTAACTACT TCACCACCTT GTCTAACAGT 
GTAGTAAATG AACCGCCGAG ATCCTACCCA TCAAAGGAGA CTTCAAGCGC GTATGTTGAT 
AAGCAGACTA ATTGTGCTTC AACAGCAGCT AGTCCCCAGT CTCTCCCCTC TTACATTTCT 
TCTCTTTCAA AACCTCCACC TTTAATTAAG CACCAACCAG AGAGCGAAGG CTCTGCGAGC 
AAGATACCTG AGCAGCTCTC GCAGTCAGTG CAGTCTCACT CTGTAAATTC TTTTAGAAGC 
GACAGCAGGA GCCCTACTCA GTTGACAGTC TCCTCTTCAA ATACACTCCG GAGTATGCCT 
GCCTTGCATA GAGCCCCTGT GTTTCACCCC CCTGTGCACC AGAACCTGGA GAAGAAGGAA 
AGCAGCTATA GCAGCCTTTC ACCTCCAACT CTGACTCCCG TTCAGCCTGT TAATGCTGGT 
GGTGGCAAAA TACAGGAGTT GCAGAAACCA CCAACTCTAG TACCTGAGCC TAAGGAAGCT 
CAGACTGTTT ACAAGGGCAC TTCTGAACAG AATGTATCAG AGGCATGGAA GTCCAATAGT 
GCCCCAAATA ACGAAAAAGT GGATTGGCAC GTTGAAAGAA TGAGTGGAAA GTCACAGTCC 
GCTACAGCAT CTGTTATTGT GCGTCCTCCT TCTAGCACGA AATACGATAG CGTAGCAGTA 
ATGCAGTCTG CTTCCAAAGA CCGAGGCAGT GAGAGATCTT CAGCTGTGAC AAATCAAGCA 
GATTGCCTGA AAACAGCGGA AGCCAGGGAG ACTGGAAGAA TCATTCTGCC AAATATGAAC 
TCAGACAGCG CTCGCACACA GTATGAAAAG AAATTTCCAG CTGTCTCGCA AGGCAGCATT 
CCGTGTGCTG TCACCCCTAC CACAACGATG ATCTGCAGTA CCAAAACGGA TGTCACCGCA 
TCGGCAGCAA CAACTACCAG CGTGTCGAGC TGGGGTAGTT CAGAGGTGAC TTACTCCTTA 
TCGAACACGG TCCTGGCCTG CGCGCCGCTG GAGTGTACGG CCTCGAGAGC CGCAAGTCAG 
GCGGTGGCAC AGGCCCAGGA ATGCAAGGTC AGCACTCCGG CTCCGGTTAC ACCCGCCGCT 
GGCAAGGCAG GCGGTGGTGC TCAGCCCAGC TCGGGATTCT CCACCTCTAC TGACTTTGTC 
CATTTGAAAA AGCACAAGGC GGCCTTGGCT GCAGCTCAGT TTAAAAGTAG TAACACCAGT 
GAGACCGAGT CCAACTCTGT GAAAAATCAG ACATTTTCAA CCTCTCTCTC CCTAGACAGC 
ACTATCGTCT GTAATACAAT AAACAAAGCG AACTCTGTAG GCAGTGGGCA AACTTCCCAG 
ACGAGTCAGC CAAACTACCA CACTAAACTG AAAAAGGCTT GGCTAACAAG ACATTCGGAG 
GAAGATAAAA ACACTAATAA AAAAGAGAAT TCAGGGAACA GTGTCTCAGA AATTATTAAG 
CCATGTACTG TCAATTTAAT AGCTTCTACA TCAAATGATT TGCAAAATAA CATAGACGGT 
AAAATCTTGG CAGATAAGTT TGCGAAGGAA GATAAGCACC CTCGGAGAAA AGCAAAACGA 
ACTTACGAGT CTGGCTCTGA AAGTGGAGAC TCTGATGAAA GCGAGAGCAA GTCAGAGCAA 
AGGACTAAAC GGCAGCCCAA GCCAACTTAC AAAAAGAAAC AAAATGATTT GCAGAAAAAA 
AAGGGTGATA CGGAGGAAGA AGTAAAACCG AATGGTGTTC TTAGCAGGAG TGCCAAAGAG 
AAAAGCAAGC TGAAGTTACA GAGCGGCAGT AACAGCACTG GTATACCTCG CTCAGTGTTA 
AAAGACTGGC GCAAAGTAAA GAAGCTGAAG CAAACAGGAG AGTCCTTTTT GCAGGATGAT 
TCTTGCTCTG AAATAGGACC AAATTTGCAA AAATGCAGAG AATGTAGGTT AATAAGAAGT 
AAGAAAGGAG AAGAACCAAC TCACTCACCA GTATTTTGTA GATTTTACTA CTTTCGGCGG 
TTATCTTTCA GTAAGAATGG AGTGGTTAGG ATAGATGGTT TCTCCTCTCC TGATCAATAT 
GATGATGAAG CACTAAGTTT ATGGACGCAT GAAAACTATG AAGATGATGA ACTGGACCTA 
GAAACTTCTA AATACATTCT AGATATCATA GGTGATAAAT TTTGTCAGTT GGTAACGTCT 
GAGAAAACAG CCATGTCCTG GGTGAAGAAA GACGCCAAAA TTGCATGGAA GAGAGCAGTG 
AGAGGAGTCC GTGAGATGTG TGATGCATGT GAAGCCACAT TATTTAACAT TCATTGGGTC 
TGCCAAAAAT GTGGATTTGT GGTCTGCCTA GATTGTTACA AAGCAAAGGA AAGAAAAAGT 
TCTAGAGATA AAGAGTTATA TGCCTGGATG AAGTGTGTGA AGGGACAGCC TCACGATCAC 
AAACACCTGA TGCCAACACA GATTATTCCA GGCTCTGTTT TGACAGACCT TCTAGATGCT 
ATGCACAGTA TTAGAGAAAA ATTTGAAATT AAATCCCATT GTCAGTGTAC CAGCAAGCAG 
AGCACACAAG CTGGCAAGCT CCCTGCAATG AATGGTGTGT CTCAGGTTTT GCAGAATGTC 
CTCAACCACA GTAATAAAAT TTCTCTGTGC ATGCCTGAGT CTCAACAGCA GAATACTCCT 
CAAAAGTCTG AGACAAATGG TAATACAAGT CCTAGAAGCG ATGTAAGCAC AGACAGCAAG 
TTAACACCTC CTGAATCCCA GTCACCGCTG CACTGGTTAG CTGATCTTGC AGAGCAGAAA 
GCCAGAGAAG AAAAGAAAGA GAACAAGGAA TGTCCTTCTG GAAAACATTC AAAGGAAGAT 
AAAGACCAGG ATAATTTGGA GTCCCAGAAC TGTAAAAGCT CCCCTCCTGC CTCCCAGAAC 
AACGAGCAGG GCTCAACCTT ACGAGATTTA TTAACTACAA CAGCAGGCAA ACTGCGCCTG 
GGTTCTACAG ATGCTGGGAT TGCTTTTGCT CCAGTTTACT CTACAGGAAC AGCAAGTGGC 
AAGAGTGGAA GGACTATGCC AAACATTCTT GATGATATAA TTGCTTCAGT AGTAGAAAAC 
AAGATTCCAC CAAATAGAGC ACCAAAGATA AATGTAAAAT CTGAAATAAA AGACGAGCCA 
AAGGACGACA AAAAATGTGT TCAGGATGAC TGCAGTAAAC TGTACAGTGA TATTCAGTAT 
TCATGGATCT GTGATAAGCA TGTTTTGTGG CTCAGAGACC ATAAAAACAA CAACAACGGG 
AAGCTCTTCA AGGAGTGTTG GAGACAAGGA AGGCCTGTTC TAGTGTCCGG TATGCATAAG 
AAAATGAACT TCAGCCTGTG GAAAGCTGAG TCAATTAGTC TAGACTTTGG AAACCAGCAA 
GCTGATATCT TGAATTGCAA AGACAGTATT ATTTCAAACA CCAATGTCAA GGAGTTCTGG 
GATGGTTTTG AAGATGTTTC AAAACGGCAG AAAATAAAAA ATGGGGAAAC AGCTCTACTG 
AAACTGAAAG ACTGGCCTTC TGGTGAAGAT TTCAAGGCTA TGATGCCAGC AAGGTATGAG 
GACTTATTAA AAAGTTTACC CTTGCCTGAA TACTGTAGTC CAGAAGGAAA GCTAAACCTG 
GCTTCTCATC TGCCGGGATT TTTTGTCCGT CCGGATTTGG GACCCAGACT GTGCAGTGCC 
TATGGTGTGG CTGCGACTAA AGACCATGAT ATAGGAACCA CAAATCTCCA TATTGAAGTT 
TCTGATGTTG TGAACATCCT TGTTTACGTT GGCATAGCAA AAGGAAATGG AGTGCTTTCC 
AAATCAGGAG TTTTGAAGAA GTTTGAAGAG GAAGATTTGG ATGACCTTTT AAGGAAGCGG 
TTGAAAGATT CAAGTGAATT ACCTGGTGCT TTATGGCACA TTTATGCTGG CAAAGACGCT 
GACAAGATAA GGGAGTTTCT GCAAAAGATA GCAAAAGAGC AAGGCTTAGA AGTTTTACCG 
GAGCATGATC CGATACGTGA TCAGAGTTGG TACGTGAACA AAAAACTTCG CCAAAGACTT 
CTTGAAGAAT ATGGAGTGAA AACCTGCACG GTTATTCAGT TTCTTGGTGA TGCTATTATT 
CTACCAGCAG GAGCACTTCA TCAGGTGCAA AATTTTCACA GCTGCGTTCA AGTAACTGAA 
GACTTTGTGT CTCCAGAACA TCTTGTACAG TCCTTTCACC TAACGCAGGA GCTGCGGCTG 
TCAAAGGAAG AAATCAATTA TGATGATAAA CTGCAGGTTA AAAATATCTT GTATCATGCA 
GTTAAAGAAA TGGTGAGAGC TCTGAAGATC CATGAAGGTG AAATGGAAGA TATGGATGAA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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JMJD1C cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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