BUB1B cDNA ORF clone, Calidris pugnax(ruff)

The following BUB1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa64064 XM_014940629.1
Latest version!
Calidris pugnax BUB1B, mitotic checkpoint serine/threonine kinase (BUB1B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa64064 XM_014940630.1
Latest version!
Calidris pugnax BUB1B, mitotic checkpoint serine/threonine kinase (BUB1B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa64064 XM_014940627.1
Latest version!
Calidris pugnax BUB1B, mitotic checkpoint serine/threonine kinase (BUB1B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa64064 XM_014940628.1
Latest version!
Calidris pugnax BUB1B, mitotic checkpoint serine/threonine kinase (BUB1B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa64064
    Clone ID Related Accession (Same CDS sequence) XM_014940627.1 , XM_014940628.1 , XM_014940630.1 , XM_014940629.1
    Accession Version XM_014940627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090829.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACAGG ACTGCAACGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTCCGG 
    CAGGGCCGTG TCATGTCCAC CTTGCAGGAA GCACTGGCTC AGCAGGAGGC CTCCAGCCAC
    ACTGCTGTTC AGCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT
    GATCCCCTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAGGGT
    GGAAAGGAGA GTAATCTCTC TGCAATACTG GAAAGGGCAG TGAAAGCGCT CAATGAACAG
    CGGCAATACT ACAACGATCC TCGCTATCTC AACCTCTGGC TCAAGTTTGG AGATCTTTGT
    AACGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCC
    CTTCTCTACA TCACCTGGGC AGAAGTACTG GAGGCAAGAG GAAGTTTTAA GAAAGCAGAT
    CTGATATTCC AGGAAGGTCT TCAGCGCAAG GCTGAGCCTT TGGACAAACT CCAGTCCCAT
    CACAGGCAGT TTCAGACCCG TGTTTCTCGG CAGGCACTAC TGGGGCTTGA GGAAACTTCT
    GATGAAAGTG ATACAGGTCT TCTGGGAGCT GCAGAACCTC AGAGAAGCTC ATTGGCAGAA
    CTAAAAGGCA GGGGGAAGAA AAAAGTGAGA GCCCCAATTA GTCGTGTAGG AGATGCACTT
    AAAGCCAGGA ACCAAACCAG AAGCTTCCAG ACTCCAACTT CCCAGCAACT TTCAAATAAC
    GCGGGTTTTG CTGTGTTTGA TGAAAATTCA GCTTCTGGAC CTGAGATTCC TGTGGTTACA
    CCACAGCCAT GGGCAGCACC TCCAGCTCCG AGGGCAAAGG AGAATGAACT GAGTGCAGGA
    CCTTGGAACT CCGGCAGGCG TCCTCGCAGG GGTGCACATT CCGGTTCAGA AGTGCCCTAC
    CCACTGCCCA GTTTCACCCC GTATGTGGAA GAGTCAGCTC AGCAGCAAGT CATGACTCCC
    TGCAAAATTG AACCCACCAT AAACCATGTT TTAAGCGCTC GCAAACCTGA GAAAGAGGAG
    GATCCACTAC AGCGAGTGCA AAATCACCAG CAGGATACTC AAGAAAAGAA AGAGATGGTG
    ATGTACTGTA AAGAGAAGGT TTATGCTGGA GTAGAAGAGT TTTCTTTGGA AGAGATTCGA
    GCTGAAATCT ACAGAAAGAA AGCAAAAAAG AAAACTGAAG AGGAGATGCA GGCCATAGCA
    CAGAAGAAGG AAGAAATACA AAAGAAAATT GAGGAGCTGG AGAAGAAATT AAAGAAGAAA
    GAGGACAAGC AGCAACAACC ATGTGAACAG CCAACAGAGA TAATGGAAGC TTCACCACCT
    TTGGGACTGC AAGGATTTAC TTCTTCCAGC GCAACAGAAG CTGGGGAGAA GCAGCCTCAG
    TTGCAAAGTG AATTCCAGGT CTCTGAAGAT ACTAAGCTAC ATAAACCATC TTGTTCTGAA
    GAGGTTTTCC AGTCTCCTGA TGTATGCCTG GATGCACAAA CGGGAAATGT GTTTTTAGAC
    TCGGAGGATG AACAGGAGAA ACAGAGAGAT GAGACCAGCC CTGTTAAAAA AGATGTAGGT
    CATCTTCTCC AACCAGATCC TGCTACGTTT TTCTCCATAT TTGATGAATC TTCTACTTTG
    GCAAATCAGA ATATAAGTTG TTCTGCAGAT CGTACACAGA AAAGTGCCCG GCGCCCTCTT
    GCTGTTCGGA AACCTTCAGA TTCCCTAGCT GCAAAGGAGA ACATATCCCC AGAAGCCTGT
    GATGATCTGA ATGGAATTGA GCCTTTGACT GAAGATGCAA TAGTGACGGG CTCCTATAAG
    CACAAAACCC TCTGTGCCAA CCCAGAAGAC ACATGTGACT TTGTCAGGGC TGCCCATCTG
    GCCTCAACAC CCTTCCATGG GGTGGCAGCT CAGAGAGTCC CAGATCCGGC TTTTCCACAG
    GCTCTCCTGA AGGAAGACTG TCCAGAACCA AAGGGTGCCC CTCTGAATCA GGAAACCCTG
    GTTTGTGAGG GAGCATACAA CGAAGCTCTT TGCATAAAAA AACTGAGCCC AATCATGGAA
    GCAAGCGTGG AGGATACTCG CTCGTCAGGT TCTTCTGTCT CCTCAGGATC TTCTCTTTCC
    TCTGTTACAC AAATATCTGC TATCAAATAC CTGCATATGC CTGAGAAACT GGAACTTGCT
    CAGAGCTTGC CTGCCGAAAT GGTTACTGAT TCTGGAGGTC TCACTGAACA CATCACTGAT
    GATGATGAAA CTCAGTTCTT GTGGAGTGCT GAACAGCGTA AAAAGCTTTT AGATCCTATG
    CCAGAGTCAC TGACCCTTTC TCCAGCTTTT CACCTGGAGC CCAGTGCTCT GCCAGCTATG
    GAGCTGGAGA AGGACGTGGA GCTCGGAAAT GAGATGTACT GCATCAAGTG GGAATATTGG
    ACCAATGAAG AGTACAAGAT GTTTTTTGCT GTTCCAGCTA ACTTTCCCCA GTTGGATGCA
    AAGGGATTTG CAATAAAGGT GTATTCTCAG CCTGTGCCTT GGGATTTTTA TATCACACTC
    CAGTTACAGG AGCGTTTGAA CACGGACTTT GACCAAAGCT TCAGTGAGAG CTGCAGCTGT
    TACATGTATC GCGATGGCTG TGCTGTCCTG CACAGGGATA TCAACCGCTT CACACTTGGG
    GATGTTATTC GTGAGTGTAA GTCCATCACA AAGGAAGTTA TCCTACTGGT TGTTTATAGT
    CTTCTGGGTG TGGTAGAGAA GCTCCACAAG GCAGAGATTG TCCATGGAGA CCTGAGCCCA
    GAGGTGTTCT TCCTGGGAGA CAGGATCTGC GATCCCTTTG CTAACGATGA GATGGCAAGA
    GCTCTGAAGA TAGTGGATTT CTCTCACAGT CTAGATTTGA GATTACAGGC TCAAGTAAGT
    TTGCCCAACA GCTTCCCAAT ATGTCAGACC CCTCACGGAC AACAGCTTCT GGTGAAAAGT
    TCTCTTCCTT ATCAGGTGGA CTTGGTTGGC ATTGCAGATA TTGTCCATTT GATGCTGTTT
    GGGGATCATG TCCAGGTCTA CCAAGAAGAC TCTATCTGGA AACTCAGCCA AGATCTATCA
    AAGACTGTTG ACAGTGATTT CTGGAGTAAA CTTTTGGGGA GACTCCTGAA CGCAGATGGT
    AAGTCCACAG TGCCTCTGCT GAGGGAGCTA AGAGAGGAAA TAAGTGGCAT GTTTGACAGC
    CATTTCCAAG AACGTCTCTG TGAATCCTTA GCTGCCCTGG GAGAGACTTT CCTCTTGGAG
    AACTTCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014796113.1
    CDS116..3427
    Translation

    Target ORF information:

    RefSeq Version XM_014940627.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax BUB1B, mitotic checkpoint serine/threonine kinase (BUB1B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014940627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACAGG ACTGCAACGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTCCGG 
    CAGGGCCGTG TCATGTCCAC CTTGCAGGAA GCACTGGCTC AGCAGGAGGC CTCCAGCCAC
    ACTGCTGTTC AGCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT
    GATCCCCTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAGGGT
    GGAAAGGAGA GTAATCTCTC TGCAATACTG GAAAGGGCAG TGAAAGCGCT CAATGAACAG
    CGGCAATACT ACAACGATCC TCGCTATCTC AACCTCTGGC TCAAGTTTGG AGATCTTTGT
    AACGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCC
    CTTCTCTACA TCACCTGGGC AGAAGTACTG GAGGCAAGAG GAAGTTTTAA GAAAGCAGAT
    CTGATATTCC AGGAAGGTCT TCAGCGCAAG GCTGAGCCTT TGGACAAACT CCAGTCCCAT
    CACAGGCAGT TTCAGACCCG TGTTTCTCGG CAGGCACTAC TGGGGCTTGA GGAAACTTCT
    GATGAAAGTG ATACAGGTCT TCTGGGAGCT GCAGAACCTC AGAGAAGCTC ATTGGCAGAA
    CTAAAAGGCA GGGGGAAGAA AAAAGTGAGA GCCCCAATTA GTCGTGTAGG AGATGCACTT
    AAAGCCAGGA ACCAAACCAG AAGCTTCCAG ACTCCAACTT CCCAGCAACT TTCAAATAAC
    GCGGGTTTTG CTGTGTTTGA TGAAAATTCA GCTTCTGGAC CTGAGATTCC TGTGGTTACA
    CCACAGCCAT GGGCAGCACC TCCAGCTCCG AGGGCAAAGG AGAATGAACT GAGTGCAGGA
    CCTTGGAACT CCGGCAGGCG TCCTCGCAGG GGTGCACATT CCGGTTCAGA AGTGCCCTAC
    CCACTGCCCA GTTTCACCCC GTATGTGGAA GAGTCAGCTC AGCAGCAAGT CATGACTCCC
    TGCAAAATTG AACCCACCAT AAACCATGTT TTAAGCGCTC GCAAACCTGA GAAAGAGGAG
    GATCCACTAC AGCGAGTGCA AAATCACCAG CAGGATACTC AAGAAAAGAA AGAGATGGTG
    ATGTACTGTA AAGAGAAGGT TTATGCTGGA GTAGAAGAGT TTTCTTTGGA AGAGATTCGA
    GCTGAAATCT ACAGAAAGAA AGCAAAAAAG AAAACTGAAG AGGAGATGCA GGCCATAGCA
    CAGAAGAAGG AAGAAATACA AAAGAAAATT GAGGAGCTGG AGAAGAAATT AAAGAAGAAA
    GAGGACAAGC AGCAACAACC ATGTGAACAG CCAACAGAGA TAATGGAAGC TTCACCACCT
    TTGGGACTGC AAGGATTTAC TTCTTCCAGC GCAACAGAAG CTGGGGAGAA GCAGCCTCAG
    TTGCAAAGTG AATTCCAGGT CTCTGAAGAT ACTAAGCTAC ATAAACCATC TTGTTCTGAA
    GAGGTTTTCC AGTCTCCTGA TGTATGCCTG GATGCACAAA CGGGAAATGT GTTTTTAGAC
    TCGGAGGATG AACAGGAGAA ACAGAGAGAT GAGACCAGCC CTGTTAAAAA AGATGTAGGT
    CATCTTCTCC AACCAGATCC TGCTACGTTT TTCTCCATAT TTGATGAATC TTCTACTTTG
    GCAAATCAGA ATATAAGTTG TTCTGCAGAT CGTACACAGA AAAGTGCCCG GCGCCCTCTT
    GCTGTTCGGA AACCTTCAGA TTCCCTAGCT GCAAAGGAGA ACATATCCCC AGAAGCCTGT
    GATGATCTGA ATGGAATTGA GCCTTTGACT GAAGATGCAA TAGTGACGGG CTCCTATAAG
    CACAAAACCC TCTGTGCCAA CCCAGAAGAC ACATGTGACT TTGTCAGGGC TGCCCATCTG
    GCCTCAACAC CCTTCCATGG GGTGGCAGCT CAGAGAGTCC CAGATCCGGC TTTTCCACAG
    GCTCTCCTGA AGGAAGACTG TCCAGAACCA AAGGGTGCCC CTCTGAATCA GGAAACCCTG
    GTTTGTGAGG GAGCATACAA CGAAGCTCTT TGCATAAAAA AACTGAGCCC AATCATGGAA
    GCAAGCGTGG AGGATACTCG CTCGTCAGGT TCTTCTGTCT CCTCAGGATC TTCTCTTTCC
    TCTGTTACAC AAATATCTGC TATCAAATAC CTGCATATGC CTGAGAAACT GGAACTTGCT
    CAGAGCTTGC CTGCCGAAAT GGTTACTGAT TCTGGAGGTC TCACTGAACA CATCACTGAT
    GATGATGAAA CTCAGTTCTT GTGGAGTGCT GAACAGCGTA AAAAGCTTTT AGATCCTATG
    CCAGAGTCAC TGACCCTTTC TCCAGCTTTT CACCTGGAGC CCAGTGCTCT GCCAGCTATG
    GAGCTGGAGA AGGACGTGGA GCTCGGAAAT GAGATGTACT GCATCAAGTG GGAATATTGG
    ACCAATGAAG AGTACAAGAT GTTTTTTGCT GTTCCAGCTA ACTTTCCCCA GTTGGATGCA
    AAGGGATTTG CAATAAAGGT GTATTCTCAG CCTGTGCCTT GGGATTTTTA TATCACACTC
    CAGTTACAGG AGCGTTTGAA CACGGACTTT GACCAAAGCT TCAGTGAGAG CTGCAGCTGT
    TACATGTATC GCGATGGCTG TGCTGTCCTG CACAGGGATA TCAACCGCTT CACACTTGGG
    GATGTTATTC GTGAGTGTAA GTCCATCACA AAGGAAGTTA TCCTACTGGT TGTTTATAGT
    CTTCTGGGTG TGGTAGAGAA GCTCCACAAG GCAGAGATTG TCCATGGAGA CCTGAGCCCA
    GAGGTGTTCT TCCTGGGAGA CAGGATCTGC GATCCCTTTG CTAACGATGA GATGGCAAGA
    GCTCTGAAGA TAGTGGATTT CTCTCACAGT CTAGATTTGA GATTACAGGC TCAAGTAAGT
    TTGCCCAACA GCTTCCCAAT ATGTCAGACC CCTCACGGAC AACAGCTTCT GGTGAAAAGT
    TCTCTTCCTT ATCAGGTGGA CTTGGTTGGC ATTGCAGATA TTGTCCATTT GATGCTGTTT
    GGGGATCATG TCCAGGTCTA CCAAGAAGAC TCTATCTGGA AACTCAGCCA AGATCTATCA
    AAGACTGTTG ACAGTGATTT CTGGAGTAAA CTTTTGGGGA GACTCCTGAA CGCAGATGGT
    AAGTCCACAG TGCCTCTGCT GAGGGAGCTA AGAGAGGAAA TAAGTGGCAT GTTTGACAGC
    CATTTCCAAG AACGTCTCTG TGAATCCTTA GCTGCCCTGG GAGAGACTTT CCTCTTGGAG
    AACTTCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa64064
    Clone ID Related Accession (Same CDS sequence) XM_014940627.1 , XM_014940628.1 , XM_014940630.1 , XM_014940629.1
    Accession Version XM_014940628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090829.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACAGG ACTGCAACGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTCCGG 
    CAGGGCCGTG TCATGTCCAC CTTGCAGGAA GCACTGGCTC AGCAGGAGGC CTCCAGCCAC
    ACTGCTGTTC AGCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT
    GATCCCCTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAGGGT
    GGAAAGGAGA GTAATCTCTC TGCAATACTG GAAAGGGCAG TGAAAGCGCT CAATGAACAG
    CGGCAATACT ACAACGATCC TCGCTATCTC AACCTCTGGC TCAAGTTTGG AGATCTTTGT
    AACGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCC
    CTTCTCTACA TCACCTGGGC AGAAGTACTG GAGGCAAGAG GAAGTTTTAA GAAAGCAGAT
    CTGATATTCC AGGAAGGTCT TCAGCGCAAG GCTGAGCCTT TGGACAAACT CCAGTCCCAT
    CACAGGCAGT TTCAGACCCG TGTTTCTCGG CAGGCACTAC TGGGGCTTGA GGAAACTTCT
    GATGAAAGTG ATACAGGTCT TCTGGGAGCT GCAGAACCTC AGAGAAGCTC ATTGGCAGAA
    CTAAAAGGCA GGGGGAAGAA AAAAGTGAGA GCCCCAATTA GTCGTGTAGG AGATGCACTT
    AAAGCCAGGA ACCAAACCAG AAGCTTCCAG ACTCCAACTT CCCAGCAACT TTCAAATAAC
    GCGGGTTTTG CTGTGTTTGA TGAAAATTCA GCTTCTGGAC CTGAGATTCC TGTGGTTACA
    CCACAGCCAT GGGCAGCACC TCCAGCTCCG AGGGCAAAGG AGAATGAACT GAGTGCAGGA
    CCTTGGAACT CCGGCAGGCG TCCTCGCAGG GGTGCACATT CCGGTTCAGA AGTGCCCTAC
    CCACTGCCCA GTTTCACCCC GTATGTGGAA GAGTCAGCTC AGCAGCAAGT CATGACTCCC
    TGCAAAATTG AACCCACCAT AAACCATGTT TTAAGCGCTC GCAAACCTGA GAAAGAGGAG
    GATCCACTAC AGCGAGTGCA AAATCACCAG CAGGATACTC AAGAAAAGAA AGAGATGGTG
    ATGTACTGTA AAGAGAAGGT TTATGCTGGA GTAGAAGAGT TTTCTTTGGA AGAGATTCGA
    GCTGAAATCT ACAGAAAGAA AGCAAAAAAG AAAACTGAAG AGGAGATGCA GGCCATAGCA
    CAGAAGAAGG AAGAAATACA AAAGAAAATT GAGGAGCTGG AGAAGAAATT AAAGAAGAAA
    GAGGACAAGC AGCAACAACC ATGTGAACAG CCAACAGAGA TAATGGAAGC TTCACCACCT
    TTGGGACTGC AAGGATTTAC TTCTTCCAGC GCAACAGAAG CTGGGGAGAA GCAGCCTCAG
    TTGCAAAGTG AATTCCAGGT CTCTGAAGAT ACTAAGCTAC ATAAACCATC TTGTTCTGAA
    GAGGTTTTCC AGTCTCCTGA TGTATGCCTG GATGCACAAA CGGGAAATGT GTTTTTAGAC
    TCGGAGGATG AACAGGAGAA ACAGAGAGAT GAGACCAGCC CTGTTAAAAA AGATGTAGGT
    CATCTTCTCC AACCAGATCC TGCTACGTTT TTCTCCATAT TTGATGAATC TTCTACTTTG
    GCAAATCAGA ATATAAGTTG TTCTGCAGAT CGTACACAGA AAAGTGCCCG GCGCCCTCTT
    GCTGTTCGGA AACCTTCAGA TTCCCTAGCT GCAAAGGAGA ACATATCCCC AGAAGCCTGT
    GATGATCTGA ATGGAATTGA GCCTTTGACT GAAGATGCAA TAGTGACGGG CTCCTATAAG
    CACAAAACCC TCTGTGCCAA CCCAGAAGAC ACATGTGACT TTGTCAGGGC TGCCCATCTG
    GCCTCAACAC CCTTCCATGG GGTGGCAGCT CAGAGAGTCC CAGATCCGGC TTTTCCACAG
    GCTCTCCTGA AGGAAGACTG TCCAGAACCA AAGGGTGCCC CTCTGAATCA GGAAACCCTG
    GTTTGTGAGG GAGCATACAA CGAAGCTCTT TGCATAAAAA AACTGAGCCC AATCATGGAA
    GCAAGCGTGG AGGATACTCG CTCGTCAGGT TCTTCTGTCT CCTCAGGATC TTCTCTTTCC
    TCTGTTACAC AAATATCTGC TATCAAATAC CTGCATATGC CTGAGAAACT GGAACTTGCT
    CAGAGCTTGC CTGCCGAAAT GGTTACTGAT TCTGGAGGTC TCACTGAACA CATCACTGAT
    GATGATGAAA CTCAGTTCTT GTGGAGTGCT GAACAGCGTA AAAAGCTTTT AGATCCTATG
    CCAGAGTCAC TGACCCTTTC TCCAGCTTTT CACCTGGAGC CCAGTGCTCT GCCAGCTATG
    GAGCTGGAGA AGGACGTGGA GCTCGGAAAT GAGATGTACT GCATCAAGTG GGAATATTGG
    ACCAATGAAG AGTACAAGAT GTTTTTTGCT GTTCCAGCTA ACTTTCCCCA GTTGGATGCA
    AAGGGATTTG CAATAAAGGT GTATTCTCAG CCTGTGCCTT GGGATTTTTA TATCACACTC
    CAGTTACAGG AGCGTTTGAA CACGGACTTT GACCAAAGCT TCAGTGAGAG CTGCAGCTGT
    TACATGTATC GCGATGGCTG TGCTGTCCTG CACAGGGATA TCAACCGCTT CACACTTGGG
    GATGTTATTC GTGAGTGTAA GTCCATCACA AAGGAAGTTA TCCTACTGGT TGTTTATAGT
    CTTCTGGGTG TGGTAGAGAA GCTCCACAAG GCAGAGATTG TCCATGGAGA CCTGAGCCCA
    GAGGTGTTCT TCCTGGGAGA CAGGATCTGC GATCCCTTTG CTAACGATGA GATGGCAAGA
    GCTCTGAAGA TAGTGGATTT CTCTCACAGT CTAGATTTGA GATTACAGGC TCAAGTAAGT
    TTGCCCAACA GCTTCCCAAT ATGTCAGACC CCTCACGGAC AACAGCTTCT GGTGAAAAGT
    TCTCTTCCTT ATCAGGTGGA CTTGGTTGGC ATTGCAGATA TTGTCCATTT GATGCTGTTT
    GGGGATCATG TCCAGGTCTA CCAAGAAGAC TCTATCTGGA AACTCAGCCA AGATCTATCA
    AAGACTGTTG ACAGTGATTT CTGGAGTAAA CTTTTGGGGA GACTCCTGAA CGCAGATGGT
    AAGTCCACAG TGCCTCTGCT GAGGGAGCTA AGAGAGGAAA TAAGTGGCAT GTTTGACAGC
    CATTTCCAAG AACGTCTCTG TGAATCCTTA GCTGCCCTGG GAGAGACTTT CCTCTTGGAG
    AACTTCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014796114.1
    CDS213..3524
    Translation

    Target ORF information:

    RefSeq Version XM_014940628.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax BUB1B, mitotic checkpoint serine/threonine kinase (BUB1B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014940628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACAGG ACTGCAACGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTCCGG 
    CAGGGCCGTG TCATGTCCAC CTTGCAGGAA GCACTGGCTC AGCAGGAGGC CTCCAGCCAC
    ACTGCTGTTC AGCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT
    GATCCCCTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAGGGT
    GGAAAGGAGA GTAATCTCTC TGCAATACTG GAAAGGGCAG TGAAAGCGCT CAATGAACAG
    CGGCAATACT ACAACGATCC TCGCTATCTC AACCTCTGGC TCAAGTTTGG AGATCTTTGT
    AACGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCC
    CTTCTCTACA TCACCTGGGC AGAAGTACTG GAGGCAAGAG GAAGTTTTAA GAAAGCAGAT
    CTGATATTCC AGGAAGGTCT TCAGCGCAAG GCTGAGCCTT TGGACAAACT CCAGTCCCAT
    CACAGGCAGT TTCAGACCCG TGTTTCTCGG CAGGCACTAC TGGGGCTTGA GGAAACTTCT
    GATGAAAGTG ATACAGGTCT TCTGGGAGCT GCAGAACCTC AGAGAAGCTC ATTGGCAGAA
    CTAAAAGGCA GGGGGAAGAA AAAAGTGAGA GCCCCAATTA GTCGTGTAGG AGATGCACTT
    AAAGCCAGGA ACCAAACCAG AAGCTTCCAG ACTCCAACTT CCCAGCAACT TTCAAATAAC
    GCGGGTTTTG CTGTGTTTGA TGAAAATTCA GCTTCTGGAC CTGAGATTCC TGTGGTTACA
    CCACAGCCAT GGGCAGCACC TCCAGCTCCG AGGGCAAAGG AGAATGAACT GAGTGCAGGA
    CCTTGGAACT CCGGCAGGCG TCCTCGCAGG GGTGCACATT CCGGTTCAGA AGTGCCCTAC
    CCACTGCCCA GTTTCACCCC GTATGTGGAA GAGTCAGCTC AGCAGCAAGT CATGACTCCC
    TGCAAAATTG AACCCACCAT AAACCATGTT TTAAGCGCTC GCAAACCTGA GAAAGAGGAG
    GATCCACTAC AGCGAGTGCA AAATCACCAG CAGGATACTC AAGAAAAGAA AGAGATGGTG
    ATGTACTGTA AAGAGAAGGT TTATGCTGGA GTAGAAGAGT TTTCTTTGGA AGAGATTCGA
    GCTGAAATCT ACAGAAAGAA AGCAAAAAAG AAAACTGAAG AGGAGATGCA GGCCATAGCA
    CAGAAGAAGG AAGAAATACA AAAGAAAATT GAGGAGCTGG AGAAGAAATT AAAGAAGAAA
    GAGGACAAGC AGCAACAACC ATGTGAACAG CCAACAGAGA TAATGGAAGC TTCACCACCT
    TTGGGACTGC AAGGATTTAC TTCTTCCAGC GCAACAGAAG CTGGGGAGAA GCAGCCTCAG
    TTGCAAAGTG AATTCCAGGT CTCTGAAGAT ACTAAGCTAC ATAAACCATC TTGTTCTGAA
    GAGGTTTTCC AGTCTCCTGA TGTATGCCTG GATGCACAAA CGGGAAATGT GTTTTTAGAC
    TCGGAGGATG AACAGGAGAA ACAGAGAGAT GAGACCAGCC CTGTTAAAAA AGATGTAGGT
    CATCTTCTCC AACCAGATCC TGCTACGTTT TTCTCCATAT TTGATGAATC TTCTACTTTG
    GCAAATCAGA ATATAAGTTG TTCTGCAGAT CGTACACAGA AAAGTGCCCG GCGCCCTCTT
    GCTGTTCGGA AACCTTCAGA TTCCCTAGCT GCAAAGGAGA ACATATCCCC AGAAGCCTGT
    GATGATCTGA ATGGAATTGA GCCTTTGACT GAAGATGCAA TAGTGACGGG CTCCTATAAG
    CACAAAACCC TCTGTGCCAA CCCAGAAGAC ACATGTGACT TTGTCAGGGC TGCCCATCTG
    GCCTCAACAC CCTTCCATGG GGTGGCAGCT CAGAGAGTCC CAGATCCGGC TTTTCCACAG
    GCTCTCCTGA AGGAAGACTG TCCAGAACCA AAGGGTGCCC CTCTGAATCA GGAAACCCTG
    GTTTGTGAGG GAGCATACAA CGAAGCTCTT TGCATAAAAA AACTGAGCCC AATCATGGAA
    GCAAGCGTGG AGGATACTCG CTCGTCAGGT TCTTCTGTCT CCTCAGGATC TTCTCTTTCC
    TCTGTTACAC AAATATCTGC TATCAAATAC CTGCATATGC CTGAGAAACT GGAACTTGCT
    CAGAGCTTGC CTGCCGAAAT GGTTACTGAT TCTGGAGGTC TCACTGAACA CATCACTGAT
    GATGATGAAA CTCAGTTCTT GTGGAGTGCT GAACAGCGTA AAAAGCTTTT AGATCCTATG
    CCAGAGTCAC TGACCCTTTC TCCAGCTTTT CACCTGGAGC CCAGTGCTCT GCCAGCTATG
    GAGCTGGAGA AGGACGTGGA GCTCGGAAAT GAGATGTACT GCATCAAGTG GGAATATTGG
    ACCAATGAAG AGTACAAGAT GTTTTTTGCT GTTCCAGCTA ACTTTCCCCA GTTGGATGCA
    AAGGGATTTG CAATAAAGGT GTATTCTCAG CCTGTGCCTT GGGATTTTTA TATCACACTC
    CAGTTACAGG AGCGTTTGAA CACGGACTTT GACCAAAGCT TCAGTGAGAG CTGCAGCTGT
    TACATGTATC GCGATGGCTG TGCTGTCCTG CACAGGGATA TCAACCGCTT CACACTTGGG
    GATGTTATTC GTGAGTGTAA GTCCATCACA AAGGAAGTTA TCCTACTGGT TGTTTATAGT
    CTTCTGGGTG TGGTAGAGAA GCTCCACAAG GCAGAGATTG TCCATGGAGA CCTGAGCCCA
    GAGGTGTTCT TCCTGGGAGA CAGGATCTGC GATCCCTTTG CTAACGATGA GATGGCAAGA
    GCTCTGAAGA TAGTGGATTT CTCTCACAGT CTAGATTTGA GATTACAGGC TCAAGTAAGT
    TTGCCCAACA GCTTCCCAAT ATGTCAGACC CCTCACGGAC AACAGCTTCT GGTGAAAAGT
    TCTCTTCCTT ATCAGGTGGA CTTGGTTGGC ATTGCAGATA TTGTCCATTT GATGCTGTTT
    GGGGATCATG TCCAGGTCTA CCAAGAAGAC TCTATCTGGA AACTCAGCCA AGATCTATCA
    AAGACTGTTG ACAGTGATTT CTGGAGTAAA CTTTTGGGGA GACTCCTGAA CGCAGATGGT
    AAGTCCACAG TGCCTCTGCT GAGGGAGCTA AGAGAGGAAA TAAGTGGCAT GTTTGACAGC
    CATTTCCAAG AACGTCTCTG TGAATCCTTA GCTGCCCTGG GAGAGACTTT CCTCTTGGAG
    AACTTCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa64064
    Clone ID Related Accession (Same CDS sequence) XM_014940627.1 , XM_014940628.1 , XM_014940630.1 , XM_014940629.1
    Accession Version XM_014940630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090829.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACAGG ACTGCAACGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTCCGG 
    CAGGGCCGTG TCATGTCCAC CTTGCAGGAA GCACTGGCTC AGCAGGAGGC CTCCAGCCAC
    ACTGCTGTTC AGCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT
    GATCCCCTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAGGGT
    GGAAAGGAGA GTAATCTCTC TGCAATACTG GAAAGGGCAG TGAAAGCGCT CAATGAACAG
    CGGCAATACT ACAACGATCC TCGCTATCTC AACCTCTGGC TCAAGTTTGG AGATCTTTGT
    AACGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCC
    CTTCTCTACA TCACCTGGGC AGAAGTACTG GAGGCAAGAG GAAGTTTTAA GAAAGCAGAT
    CTGATATTCC AGGAAGGTCT TCAGCGCAAG GCTGAGCCTT TGGACAAACT CCAGTCCCAT
    CACAGGCAGT TTCAGACCCG TGTTTCTCGG CAGGCACTAC TGGGGCTTGA GGAAACTTCT
    GATGAAAGTG ATACAGGTCT TCTGGGAGCT GCAGAACCTC AGAGAAGCTC ATTGGCAGAA
    CTAAAAGGCA GGGGGAAGAA AAAAGTGAGA GCCCCAATTA GTCGTGTAGG AGATGCACTT
    AAAGCCAGGA ACCAAACCAG AAGCTTCCAG ACTCCAACTT CCCAGCAACT TTCAAATAAC
    GCGGGTTTTG CTGTGTTTGA TGAAAATTCA GCTTCTGGAC CTGAGATTCC TGTGGTTACA
    CCACAGCCAT GGGCAGCACC TCCAGCTCCG AGGGCAAAGG AGAATGAACT GAGTGCAGGA
    CCTTGGAACT CCGGCAGGCG TCCTCGCAGG GGTGCACATT CCGGTTCAGA AGTGCCCTAC
    CCACTGCCCA GTTTCACCCC GTATGTGGAA GAGTCAGCTC AGCAGCAAGT CATGACTCCC
    TGCAAAATTG AACCCACCAT AAACCATGTT TTAAGCGCTC GCAAACCTGA GAAAGAGGAG
    GATCCACTAC AGCGAGTGCA AAATCACCAG CAGGATACTC AAGAAAAGAA AGAGATGGTG
    ATGTACTGTA AAGAGAAGGT TTATGCTGGA GTAGAAGAGT TTTCTTTGGA AGAGATTCGA
    GCTGAAATCT ACAGAAAGAA AGCAAAAAAG AAAACTGAAG AGGAGATGCA GGCCATAGCA
    CAGAAGAAGG AAGAAATACA AAAGAAAATT GAGGAGCTGG AGAAGAAATT AAAGAAGAAA
    GAGGACAAGC AGCAACAACC ATGTGAACAG CCAACAGAGA TAATGGAAGC TTCACCACCT
    TTGGGACTGC AAGGATTTAC TTCTTCCAGC GCAACAGAAG CTGGGGAGAA GCAGCCTCAG
    TTGCAAAGTG AATTCCAGGT CTCTGAAGAT ACTAAGCTAC ATAAACCATC TTGTTCTGAA
    GAGGTTTTCC AGTCTCCTGA TGTATGCCTG GATGCACAAA CGGGAAATGT GTTTTTAGAC
    TCGGAGGATG AACAGGAGAA ACAGAGAGAT GAGACCAGCC CTGTTAAAAA AGATGTAGGT
    CATCTTCTCC AACCAGATCC TGCTACGTTT TTCTCCATAT TTGATGAATC TTCTACTTTG
    GCAAATCAGA ATATAAGTTG TTCTGCAGAT CGTACACAGA AAAGTGCCCG GCGCCCTCTT
    GCTGTTCGGA AACCTTCAGA TTCCCTAGCT GCAAAGGAGA ACATATCCCC AGAAGCCTGT
    GATGATCTGA ATGGAATTGA GCCTTTGACT GAAGATGCAA TAGTGACGGG CTCCTATAAG
    CACAAAACCC TCTGTGCCAA CCCAGAAGAC ACATGTGACT TTGTCAGGGC TGCCCATCTG
    GCCTCAACAC CCTTCCATGG GGTGGCAGCT CAGAGAGTCC CAGATCCGGC TTTTCCACAG
    GCTCTCCTGA AGGAAGACTG TCCAGAACCA AAGGGTGCCC CTCTGAATCA GGAAACCCTG
    GTTTGTGAGG GAGCATACAA CGAAGCTCTT TGCATAAAAA AACTGAGCCC AATCATGGAA
    GCAAGCGTGG AGGATACTCG CTCGTCAGGT TCTTCTGTCT CCTCAGGATC TTCTCTTTCC
    TCTGTTACAC AAATATCTGC TATCAAATAC CTGCATATGC CTGAGAAACT GGAACTTGCT
    CAGAGCTTGC CTGCCGAAAT GGTTACTGAT TCTGGAGGTC TCACTGAACA CATCACTGAT
    GATGATGAAA CTCAGTTCTT GTGGAGTGCT GAACAGCGTA AAAAGCTTTT AGATCCTATG
    CCAGAGTCAC TGACCCTTTC TCCAGCTTTT CACCTGGAGC CCAGTGCTCT GCCAGCTATG
    GAGCTGGAGA AGGACGTGGA GCTCGGAAAT GAGATGTACT GCATCAAGTG GGAATATTGG
    ACCAATGAAG AGTACAAGAT GTTTTTTGCT GTTCCAGCTA ACTTTCCCCA GTTGGATGCA
    AAGGGATTTG CAATAAAGGT GTATTCTCAG CCTGTGCCTT GGGATTTTTA TATCACACTC
    CAGTTACAGG AGCGTTTGAA CACGGACTTT GACCAAAGCT TCAGTGAGAG CTGCAGCTGT
    TACATGTATC GCGATGGCTG TGCTGTCCTG CACAGGGATA TCAACCGCTT CACACTTGGG
    GATGTTATTC GTGAGTGTAA GTCCATCACA AAGGAAGTTA TCCTACTGGT TGTTTATAGT
    CTTCTGGGTG TGGTAGAGAA GCTCCACAAG GCAGAGATTG TCCATGGAGA CCTGAGCCCA
    GAGGTGTTCT TCCTGGGAGA CAGGATCTGC GATCCCTTTG CTAACGATGA GATGGCAAGA
    GCTCTGAAGA TAGTGGATTT CTCTCACAGT CTAGATTTGA GATTACAGGC TCAAGTAAGT
    TTGCCCAACA GCTTCCCAAT ATGTCAGACC CCTCACGGAC AACAGCTTCT GGTGAAAAGT
    TCTCTTCCTT ATCAGGTGGA CTTGGTTGGC ATTGCAGATA TTGTCCATTT GATGCTGTTT
    GGGGATCATG TCCAGGTCTA CCAAGAAGAC TCTATCTGGA AACTCAGCCA AGATCTATCA
    AAGACTGTTG ACAGTGATTT CTGGAGTAAA CTTTTGGGGA GACTCCTGAA CGCAGATGGT
    AAGTCCACAG TGCCTCTGCT GAGGGAGCTA AGAGAGGAAA TAAGTGGCAT GTTTGACAGC
    CATTTCCAAG AACGTCTCTG TGAATCCTTA GCTGCCCTGG GAGAGACTTT CCTCTTGGAG
    AACTTCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014796116.1
    CDS182..3493
    Translation

    Target ORF information:

    RefSeq Version XM_014940630.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax BUB1B, mitotic checkpoint serine/threonine kinase (BUB1B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014940630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACAGG ACTGCAACGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTCCGG 
    CAGGGCCGTG TCATGTCCAC CTTGCAGGAA GCACTGGCTC AGCAGGAGGC CTCCAGCCAC
    ACTGCTGTTC AGCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT
    GATCCCCTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAGGGT
    GGAAAGGAGA GTAATCTCTC TGCAATACTG GAAAGGGCAG TGAAAGCGCT CAATGAACAG
    CGGCAATACT ACAACGATCC TCGCTATCTC AACCTCTGGC TCAAGTTTGG AGATCTTTGT
    AACGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCC
    CTTCTCTACA TCACCTGGGC AGAAGTACTG GAGGCAAGAG GAAGTTTTAA GAAAGCAGAT
    CTGATATTCC AGGAAGGTCT TCAGCGCAAG GCTGAGCCTT TGGACAAACT CCAGTCCCAT
    CACAGGCAGT TTCAGACCCG TGTTTCTCGG CAGGCACTAC TGGGGCTTGA GGAAACTTCT
    GATGAAAGTG ATACAGGTCT TCTGGGAGCT GCAGAACCTC AGAGAAGCTC ATTGGCAGAA
    CTAAAAGGCA GGGGGAAGAA AAAAGTGAGA GCCCCAATTA GTCGTGTAGG AGATGCACTT
    AAAGCCAGGA ACCAAACCAG AAGCTTCCAG ACTCCAACTT CCCAGCAACT TTCAAATAAC
    GCGGGTTTTG CTGTGTTTGA TGAAAATTCA GCTTCTGGAC CTGAGATTCC TGTGGTTACA
    CCACAGCCAT GGGCAGCACC TCCAGCTCCG AGGGCAAAGG AGAATGAACT GAGTGCAGGA
    CCTTGGAACT CCGGCAGGCG TCCTCGCAGG GGTGCACATT CCGGTTCAGA AGTGCCCTAC
    CCACTGCCCA GTTTCACCCC GTATGTGGAA GAGTCAGCTC AGCAGCAAGT CATGACTCCC
    TGCAAAATTG AACCCACCAT AAACCATGTT TTAAGCGCTC GCAAACCTGA GAAAGAGGAG
    GATCCACTAC AGCGAGTGCA AAATCACCAG CAGGATACTC AAGAAAAGAA AGAGATGGTG
    ATGTACTGTA AAGAGAAGGT TTATGCTGGA GTAGAAGAGT TTTCTTTGGA AGAGATTCGA
    GCTGAAATCT ACAGAAAGAA AGCAAAAAAG AAAACTGAAG AGGAGATGCA GGCCATAGCA
    CAGAAGAAGG AAGAAATACA AAAGAAAATT GAGGAGCTGG AGAAGAAATT AAAGAAGAAA
    GAGGACAAGC AGCAACAACC ATGTGAACAG CCAACAGAGA TAATGGAAGC TTCACCACCT
    TTGGGACTGC AAGGATTTAC TTCTTCCAGC GCAACAGAAG CTGGGGAGAA GCAGCCTCAG
    TTGCAAAGTG AATTCCAGGT CTCTGAAGAT ACTAAGCTAC ATAAACCATC TTGTTCTGAA
    GAGGTTTTCC AGTCTCCTGA TGTATGCCTG GATGCACAAA CGGGAAATGT GTTTTTAGAC
    TCGGAGGATG AACAGGAGAA ACAGAGAGAT GAGACCAGCC CTGTTAAAAA AGATGTAGGT
    CATCTTCTCC AACCAGATCC TGCTACGTTT TTCTCCATAT TTGATGAATC TTCTACTTTG
    GCAAATCAGA ATATAAGTTG TTCTGCAGAT CGTACACAGA AAAGTGCCCG GCGCCCTCTT
    GCTGTTCGGA AACCTTCAGA TTCCCTAGCT GCAAAGGAGA ACATATCCCC AGAAGCCTGT
    GATGATCTGA ATGGAATTGA GCCTTTGACT GAAGATGCAA TAGTGACGGG CTCCTATAAG
    CACAAAACCC TCTGTGCCAA CCCAGAAGAC ACATGTGACT TTGTCAGGGC TGCCCATCTG
    GCCTCAACAC CCTTCCATGG GGTGGCAGCT CAGAGAGTCC CAGATCCGGC TTTTCCACAG
    GCTCTCCTGA AGGAAGACTG TCCAGAACCA AAGGGTGCCC CTCTGAATCA GGAAACCCTG
    GTTTGTGAGG GAGCATACAA CGAAGCTCTT TGCATAAAAA AACTGAGCCC AATCATGGAA
    GCAAGCGTGG AGGATACTCG CTCGTCAGGT TCTTCTGTCT CCTCAGGATC TTCTCTTTCC
    TCTGTTACAC AAATATCTGC TATCAAATAC CTGCATATGC CTGAGAAACT GGAACTTGCT
    CAGAGCTTGC CTGCCGAAAT GGTTACTGAT TCTGGAGGTC TCACTGAACA CATCACTGAT
    GATGATGAAA CTCAGTTCTT GTGGAGTGCT GAACAGCGTA AAAAGCTTTT AGATCCTATG
    CCAGAGTCAC TGACCCTTTC TCCAGCTTTT CACCTGGAGC CCAGTGCTCT GCCAGCTATG
    GAGCTGGAGA AGGACGTGGA GCTCGGAAAT GAGATGTACT GCATCAAGTG GGAATATTGG
    ACCAATGAAG AGTACAAGAT GTTTTTTGCT GTTCCAGCTA ACTTTCCCCA GTTGGATGCA
    AAGGGATTTG CAATAAAGGT GTATTCTCAG CCTGTGCCTT GGGATTTTTA TATCACACTC
    CAGTTACAGG AGCGTTTGAA CACGGACTTT GACCAAAGCT TCAGTGAGAG CTGCAGCTGT
    TACATGTATC GCGATGGCTG TGCTGTCCTG CACAGGGATA TCAACCGCTT CACACTTGGG
    GATGTTATTC GTGAGTGTAA GTCCATCACA AAGGAAGTTA TCCTACTGGT TGTTTATAGT
    CTTCTGGGTG TGGTAGAGAA GCTCCACAAG GCAGAGATTG TCCATGGAGA CCTGAGCCCA
    GAGGTGTTCT TCCTGGGAGA CAGGATCTGC GATCCCTTTG CTAACGATGA GATGGCAAGA
    GCTCTGAAGA TAGTGGATTT CTCTCACAGT CTAGATTTGA GATTACAGGC TCAAGTAAGT
    TTGCCCAACA GCTTCCCAAT ATGTCAGACC CCTCACGGAC AACAGCTTCT GGTGAAAAGT
    TCTCTTCCTT ATCAGGTGGA CTTGGTTGGC ATTGCAGATA TTGTCCATTT GATGCTGTTT
    GGGGATCATG TCCAGGTCTA CCAAGAAGAC TCTATCTGGA AACTCAGCCA AGATCTATCA
    AAGACTGTTG ACAGTGATTT CTGGAGTAAA CTTTTGGGGA GACTCCTGAA CGCAGATGGT
    AAGTCCACAG TGCCTCTGCT GAGGGAGCTA AGAGAGGAAA TAAGTGGCAT GTTTGACAGC
    CATTTCCAAG AACGTCTCTG TGAATCCTTA GCTGCCCTGG GAGAGACTTT CCTCTTGGAG
    AACTTCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa64064
    Clone ID Related Accession (Same CDS sequence) XM_014940627.1 , XM_014940628.1 , XM_014940630.1 , XM_014940629.1
    Accession Version XM_014940629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090829.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACAGG ACTGCAACGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTCCGG 
    CAGGGCCGTG TCATGTCCAC CTTGCAGGAA GCACTGGCTC AGCAGGAGGC CTCCAGCCAC
    ACTGCTGTTC AGCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT
    GATCCCCTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAGGGT
    GGAAAGGAGA GTAATCTCTC TGCAATACTG GAAAGGGCAG TGAAAGCGCT CAATGAACAG
    CGGCAATACT ACAACGATCC TCGCTATCTC AACCTCTGGC TCAAGTTTGG AGATCTTTGT
    AACGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCC
    CTTCTCTACA TCACCTGGGC AGAAGTACTG GAGGCAAGAG GAAGTTTTAA GAAAGCAGAT
    CTGATATTCC AGGAAGGTCT TCAGCGCAAG GCTGAGCCTT TGGACAAACT CCAGTCCCAT
    CACAGGCAGT TTCAGACCCG TGTTTCTCGG CAGGCACTAC TGGGGCTTGA GGAAACTTCT
    GATGAAAGTG ATACAGGTCT TCTGGGAGCT GCAGAACCTC AGAGAAGCTC ATTGGCAGAA
    CTAAAAGGCA GGGGGAAGAA AAAAGTGAGA GCCCCAATTA GTCGTGTAGG AGATGCACTT
    AAAGCCAGGA ACCAAACCAG AAGCTTCCAG ACTCCAACTT CCCAGCAACT TTCAAATAAC
    GCGGGTTTTG CTGTGTTTGA TGAAAATTCA GCTTCTGGAC CTGAGATTCC TGTGGTTACA
    CCACAGCCAT GGGCAGCACC TCCAGCTCCG AGGGCAAAGG AGAATGAACT GAGTGCAGGA
    CCTTGGAACT CCGGCAGGCG TCCTCGCAGG GGTGCACATT CCGGTTCAGA AGTGCCCTAC
    CCACTGCCCA GTTTCACCCC GTATGTGGAA GAGTCAGCTC AGCAGCAAGT CATGACTCCC
    TGCAAAATTG AACCCACCAT AAACCATGTT TTAAGCGCTC GCAAACCTGA GAAAGAGGAG
    GATCCACTAC AGCGAGTGCA AAATCACCAG CAGGATACTC AAGAAAAGAA AGAGATGGTG
    ATGTACTGTA AAGAGAAGGT TTATGCTGGA GTAGAAGAGT TTTCTTTGGA AGAGATTCGA
    GCTGAAATCT ACAGAAAGAA AGCAAAAAAG AAAACTGAAG AGGAGATGCA GGCCATAGCA
    CAGAAGAAGG AAGAAATACA AAAGAAAATT GAGGAGCTGG AGAAGAAATT AAAGAAGAAA
    GAGGACAAGC AGCAACAACC ATGTGAACAG CCAACAGAGA TAATGGAAGC TTCACCACCT
    TTGGGACTGC AAGGATTTAC TTCTTCCAGC GCAACAGAAG CTGGGGAGAA GCAGCCTCAG
    TTGCAAAGTG AATTCCAGGT CTCTGAAGAT ACTAAGCTAC ATAAACCATC TTGTTCTGAA
    GAGGTTTTCC AGTCTCCTGA TGTATGCCTG GATGCACAAA CGGGAAATGT GTTTTTAGAC
    TCGGAGGATG AACAGGAGAA ACAGAGAGAT GAGACCAGCC CTGTTAAAAA AGATGTAGGT
    CATCTTCTCC AACCAGATCC TGCTACGTTT TTCTCCATAT TTGATGAATC TTCTACTTTG
    GCAAATCAGA ATATAAGTTG TTCTGCAGAT CGTACACAGA AAAGTGCCCG GCGCCCTCTT
    GCTGTTCGGA AACCTTCAGA TTCCCTAGCT GCAAAGGAGA ACATATCCCC AGAAGCCTGT
    GATGATCTGA ATGGAATTGA GCCTTTGACT GAAGATGCAA TAGTGACGGG CTCCTATAAG
    CACAAAACCC TCTGTGCCAA CCCAGAAGAC ACATGTGACT TTGTCAGGGC TGCCCATCTG
    GCCTCAACAC CCTTCCATGG GGTGGCAGCT CAGAGAGTCC CAGATCCGGC TTTTCCACAG
    GCTCTCCTGA AGGAAGACTG TCCAGAACCA AAGGGTGCCC CTCTGAATCA GGAAACCCTG
    GTTTGTGAGG GAGCATACAA CGAAGCTCTT TGCATAAAAA AACTGAGCCC AATCATGGAA
    GCAAGCGTGG AGGATACTCG CTCGTCAGGT TCTTCTGTCT CCTCAGGATC TTCTCTTTCC
    TCTGTTACAC AAATATCTGC TATCAAATAC CTGCATATGC CTGAGAAACT GGAACTTGCT
    CAGAGCTTGC CTGCCGAAAT GGTTACTGAT TCTGGAGGTC TCACTGAACA CATCACTGAT
    GATGATGAAA CTCAGTTCTT GTGGAGTGCT GAACAGCGTA AAAAGCTTTT AGATCCTATG
    CCAGAGTCAC TGACCCTTTC TCCAGCTTTT CACCTGGAGC CCAGTGCTCT GCCAGCTATG
    GAGCTGGAGA AGGACGTGGA GCTCGGAAAT GAGATGTACT GCATCAAGTG GGAATATTGG
    ACCAATGAAG AGTACAAGAT GTTTTTTGCT GTTCCAGCTA ACTTTCCCCA GTTGGATGCA
    AAGGGATTTG CAATAAAGGT GTATTCTCAG CCTGTGCCTT GGGATTTTTA TATCACACTC
    CAGTTACAGG AGCGTTTGAA CACGGACTTT GACCAAAGCT TCAGTGAGAG CTGCAGCTGT
    TACATGTATC GCGATGGCTG TGCTGTCCTG CACAGGGATA TCAACCGCTT CACACTTGGG
    GATGTTATTC GTGAGTGTAA GTCCATCACA AAGGAAGTTA TCCTACTGGT TGTTTATAGT
    CTTCTGGGTG TGGTAGAGAA GCTCCACAAG GCAGAGATTG TCCATGGAGA CCTGAGCCCA
    GAGGTGTTCT TCCTGGGAGA CAGGATCTGC GATCCCTTTG CTAACGATGA GATGGCAAGA
    GCTCTGAAGA TAGTGGATTT CTCTCACAGT CTAGATTTGA GATTACAGGC TCAAGTAAGT
    TTGCCCAACA GCTTCCCAAT ATGTCAGACC CCTCACGGAC AACAGCTTCT GGTGAAAAGT
    TCTCTTCCTT ATCAGGTGGA CTTGGTTGGC ATTGCAGATA TTGTCCATTT GATGCTGTTT
    GGGGATCATG TCCAGGTCTA CCAAGAAGAC TCTATCTGGA AACTCAGCCA AGATCTATCA
    AAGACTGTTG ACAGTGATTT CTGGAGTAAA CTTTTGGGGA GACTCCTGAA CGCAGATGGT
    AAGTCCACAG TGCCTCTGCT GAGGGAGCTA AGAGAGGAAA TAAGTGGCAT GTTTGACAGC
    CATTTCCAAG AACGTCTCTG TGAATCCTTA GCTGCCCTGG GAGAGACTTT CCTCTTGGAG
    AACTTCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014796115.1
    CDS44..3355
    Translation

    Target ORF information:

    RefSeq Version XM_014940629.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax BUB1B, mitotic checkpoint serine/threonine kinase (BUB1B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014940629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACAGG ACTGCAACGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTCCGG 
    CAGGGCCGTG TCATGTCCAC CTTGCAGGAA GCACTGGCTC AGCAGGAGGC CTCCAGCCAC
    ACTGCTGTTC AGCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT
    GATCCCCTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAGGGT
    GGAAAGGAGA GTAATCTCTC TGCAATACTG GAAAGGGCAG TGAAAGCGCT CAATGAACAG
    CGGCAATACT ACAACGATCC TCGCTATCTC AACCTCTGGC TCAAGTTTGG AGATCTTTGT
    AACGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCC
    CTTCTCTACA TCACCTGGGC AGAAGTACTG GAGGCAAGAG GAAGTTTTAA GAAAGCAGAT
    CTGATATTCC AGGAAGGTCT TCAGCGCAAG GCTGAGCCTT TGGACAAACT CCAGTCCCAT
    CACAGGCAGT TTCAGACCCG TGTTTCTCGG CAGGCACTAC TGGGGCTTGA GGAAACTTCT
    GATGAAAGTG ATACAGGTCT TCTGGGAGCT GCAGAACCTC AGAGAAGCTC ATTGGCAGAA
    CTAAAAGGCA GGGGGAAGAA AAAAGTGAGA GCCCCAATTA GTCGTGTAGG AGATGCACTT
    AAAGCCAGGA ACCAAACCAG AAGCTTCCAG ACTCCAACTT CCCAGCAACT TTCAAATAAC
    GCGGGTTTTG CTGTGTTTGA TGAAAATTCA GCTTCTGGAC CTGAGATTCC TGTGGTTACA
    CCACAGCCAT GGGCAGCACC TCCAGCTCCG AGGGCAAAGG AGAATGAACT GAGTGCAGGA
    CCTTGGAACT CCGGCAGGCG TCCTCGCAGG GGTGCACATT CCGGTTCAGA AGTGCCCTAC
    CCACTGCCCA GTTTCACCCC GTATGTGGAA GAGTCAGCTC AGCAGCAAGT CATGACTCCC
    TGCAAAATTG AACCCACCAT AAACCATGTT TTAAGCGCTC GCAAACCTGA GAAAGAGGAG
    GATCCACTAC AGCGAGTGCA AAATCACCAG CAGGATACTC AAGAAAAGAA AGAGATGGTG
    ATGTACTGTA AAGAGAAGGT TTATGCTGGA GTAGAAGAGT TTTCTTTGGA AGAGATTCGA
    GCTGAAATCT ACAGAAAGAA AGCAAAAAAG AAAACTGAAG AGGAGATGCA GGCCATAGCA
    CAGAAGAAGG AAGAAATACA AAAGAAAATT GAGGAGCTGG AGAAGAAATT AAAGAAGAAA
    GAGGACAAGC AGCAACAACC ATGTGAACAG CCAACAGAGA TAATGGAAGC TTCACCACCT
    TTGGGACTGC AAGGATTTAC TTCTTCCAGC GCAACAGAAG CTGGGGAGAA GCAGCCTCAG
    TTGCAAAGTG AATTCCAGGT CTCTGAAGAT ACTAAGCTAC ATAAACCATC TTGTTCTGAA
    GAGGTTTTCC AGTCTCCTGA TGTATGCCTG GATGCACAAA CGGGAAATGT GTTTTTAGAC
    TCGGAGGATG AACAGGAGAA ACAGAGAGAT GAGACCAGCC CTGTTAAAAA AGATGTAGGT
    CATCTTCTCC AACCAGATCC TGCTACGTTT TTCTCCATAT TTGATGAATC TTCTACTTTG
    GCAAATCAGA ATATAAGTTG TTCTGCAGAT CGTACACAGA AAAGTGCCCG GCGCCCTCTT
    GCTGTTCGGA AACCTTCAGA TTCCCTAGCT GCAAAGGAGA ACATATCCCC AGAAGCCTGT
    GATGATCTGA ATGGAATTGA GCCTTTGACT GAAGATGCAA TAGTGACGGG CTCCTATAAG
    CACAAAACCC TCTGTGCCAA CCCAGAAGAC ACATGTGACT TTGTCAGGGC TGCCCATCTG
    GCCTCAACAC CCTTCCATGG GGTGGCAGCT CAGAGAGTCC CAGATCCGGC TTTTCCACAG
    GCTCTCCTGA AGGAAGACTG TCCAGAACCA AAGGGTGCCC CTCTGAATCA GGAAACCCTG
    GTTTGTGAGG GAGCATACAA CGAAGCTCTT TGCATAAAAA AACTGAGCCC AATCATGGAA
    GCAAGCGTGG AGGATACTCG CTCGTCAGGT TCTTCTGTCT CCTCAGGATC TTCTCTTTCC
    TCTGTTACAC AAATATCTGC TATCAAATAC CTGCATATGC CTGAGAAACT GGAACTTGCT
    CAGAGCTTGC CTGCCGAAAT GGTTACTGAT TCTGGAGGTC TCACTGAACA CATCACTGAT
    GATGATGAAA CTCAGTTCTT GTGGAGTGCT GAACAGCGTA AAAAGCTTTT AGATCCTATG
    CCAGAGTCAC TGACCCTTTC TCCAGCTTTT CACCTGGAGC CCAGTGCTCT GCCAGCTATG
    GAGCTGGAGA AGGACGTGGA GCTCGGAAAT GAGATGTACT GCATCAAGTG GGAATATTGG
    ACCAATGAAG AGTACAAGAT GTTTTTTGCT GTTCCAGCTA ACTTTCCCCA GTTGGATGCA
    AAGGGATTTG CAATAAAGGT GTATTCTCAG CCTGTGCCTT GGGATTTTTA TATCACACTC
    CAGTTACAGG AGCGTTTGAA CACGGACTTT GACCAAAGCT TCAGTGAGAG CTGCAGCTGT
    TACATGTATC GCGATGGCTG TGCTGTCCTG CACAGGGATA TCAACCGCTT CACACTTGGG
    GATGTTATTC GTGAGTGTAA GTCCATCACA AAGGAAGTTA TCCTACTGGT TGTTTATAGT
    CTTCTGGGTG TGGTAGAGAA GCTCCACAAG GCAGAGATTG TCCATGGAGA CCTGAGCCCA
    GAGGTGTTCT TCCTGGGAGA CAGGATCTGC GATCCCTTTG CTAACGATGA GATGGCAAGA
    GCTCTGAAGA TAGTGGATTT CTCTCACAGT CTAGATTTGA GATTACAGGC TCAAGTAAGT
    TTGCCCAACA GCTTCCCAAT ATGTCAGACC CCTCACGGAC AACAGCTTCT GGTGAAAAGT
    TCTCTTCCTT ATCAGGTGGA CTTGGTTGGC ATTGCAGATA TTGTCCATTT GATGCTGTTT
    GGGGATCATG TCCAGGTCTA CCAAGAAGAC TCTATCTGGA AACTCAGCCA AGATCTATCA
    AAGACTGTTG ACAGTGATTT CTGGAGTAAA CTTTTGGGGA GACTCCTGAA CGCAGATGGT
    AAGTCCACAG TGCCTCTGCT GAGGGAGCTA AGAGAGGAAA TAAGTGGCAT GTTTGACAGC
    CATTTCCAAG AACGTCTCTG TGAATCCTTA GCTGCCCTGG GAGAGACTTT CCTCTTGGAG
    AACTTCCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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