LOC106890484 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106890484
Entrez Gene ID	106890484
Full Name	receptor-type tyrosine-protein phosphatase V-like
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014946347.1	XP_014801833.1	receptor-type tyrosine-protein phosphatase V-like isoform X5
XM_014946344.1	XP_014801830.1	receptor-type tyrosine-protein phosphatase V-like isoform X2
XM_014946343.1	XP_014801829.1	receptor-type tyrosine-protein phosphatase V-like isoform X1
XM_014946345.1	XP_014801831.1	receptor-type tyrosine-protein phosphatase V-like isoform X3
XM_014946346.1	XP_014801832.1	receptor-type tyrosine-protein phosphatase V-like isoform X4
XM_014946348.1	XP_014801834.1	receptor-type tyrosine-protein phosphatase V-like isoform X6
XM_014946349.1	XP_014801835.1	receptor-type tyrosine-protein phosphatase V-like isoform X7

Pathways from BioSystems
Gene Ontology

The following LOC106890484 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106890484 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa68222	XM_014946346.1 Latest version!	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa68219	XM_014946343.1 Latest version!	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCa68220	XM_014946344.1 Latest version!	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCa68221	XM_014946345.1 Latest version!	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCa68223	XM_014946347.1 Latest version!	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCa68224	XM_014946348.1 Latest version!	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCa68225	XM_014946349.1 Latest version!	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106890484 ( XM_014946346.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801832.1 Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa68222
Clone ID Related Accession (Same CDS sequence)	XM_014946346.1
Accession Version	XM_014946346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	receptor-type tyrosine-protein phosphatase V-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACCGC CGCCGCCGCT GCTGCTGCTC GTCCTGTGGG TGCTGCGGCT CCTGGGAACC 
CTGGCAGAGG ATGAGGGATG CAACCAGACA GCACGGGCAG GGTTGGAGGG ACAGGATGCA 
CCCAATGATA GAGGGACCCT CCTGAACCTC AGTGTGAGTG ACCATGGCTG GTCGGACTCC 
CTCCTTCTGT CCTGGGATGA GCCAGAGGGG GGTGCCAAAG GATATTCGCT CGCTCTCTCC 
TCCCTGGGGT CAGGCACACC GTTGCAGAAT GGATCTGCTG GACCCAACAT CACCAGCTTC 
TGGTTCCATG GGCTGACCCC TGGCATGCGC TATGAGATTG AGGTGACAGC CACACTTGCC 
TGCATGGAGA CCACCAGCCA GACAGTCACA GCACAGACAA GTCCTTCACC TGTCCGCAAC 
CTGACCCTGA GCAGTGGTGG GAGCTCTGCC ATGCTGCAGG CGTCCTGGGC GCATGCCCAC 
GGGCAGCGAG ACAGCTACCA CCTAGCCCTC TACCACAGCG ACTCCCAGAC GCTGGTGAGA 
AATGTGTCCA TCCTGCCAAA CGCCTCTACC TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT 
GAGTACGCCT TGAAGGTCAG CACGCTGGTT GGATCCAGTC AGGCGAGCAC CAGTATCCAC 
CAGTGGACAG CTCCCTCCAT CCCCACCCAG CTGAGACTCA GCCCAGGATC CAGCACCAGC 
CTCATCGCCT CCTGGGCAGA TGCTGGGGGG GCTGCCTGGC TGCACCTCAC ACTCCACAAC 
CTCCTCACCC AGATGGTGAC CACCACCCTG TCAGCCAGAA GGGGCCTCAC CAGCTACACC 
TTCCAGCATC TCCACTCCGG CACCCAGTAC TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC 
TACACCATGG TGGGGCCCAA TGCCACCGCT TGGACATACC CCCTGAGCCC TGGAAATGTG 
ACCCTGAGCC CTGCAGAGGA ACAGAACTCC TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA 
GAGAGGGACT TCTACCTGGT GACACTGCAG GAGGAAGAGG ATGGTGTGCC AACAAGGAAC 
ATCTCTGTCG ATGGAGACAA CAGTCATGTC ACTTTCCACG GGCTGGATCC TGGGAAGCAA 
TACTCTGTCC AGGTGACAGC AGTGGCTGGG CCTTACAGAG CATCAGCCCA CAGCACAGCA 
GCCTGGACAC AGCCGCTAGC ACCATCTGGT GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC 
AGCCTACTGG CCAGCTGGGA GGAGGCAATG GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC 
ATGGAGCACC CTGTGAAGAA TAGCTCGCTG GTTAAAGGTG TCACCAACTT CACCTACAAC 
GGCCTCCACC CTGGGACTCT TTACACCTTT GAGGTCAGCA CCGTGGCTGG CCCCTACACA 
TCCTCGCCCC GGCGCATCAC CAACTGGACA TATCCTTTGC CCCTGGAGCA GCTGACCCTC 
AGCAATCAGG GCCACAGCAC CTCTCTGCAA GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC 
ACTGGCTACG CAGGCACCCT CTGGGAAACC AAGTCCCAGG AGCGGGTCAG GAACATGACT 
GTGGGGAACA ACTGGATGAA TGCCACCTTT GAGGACCTGG TCCCTGGGCG GCAGTATACA 
CTGGAGATGA CTGCTATGGC CGGACCTTAC AGATCCCCTG TGCGATCAGC CACAGACTGG 
ACATACCCTC TGGCTCCAGC CGGCGTGACC CTGACCAACA CCCGACGCCC ACTGGGACTT 
GCTGCCTTCT GGGACAAGGC TGCTGGTGAT GTGGACCAGT TCCACCTCCA GCTCTACAGC 
AAGAGCCATC CAGCACAGAG GAACATCTCA GTAGGGCCAG ACACCCACAA CTTCACATTC 
CTAGGGCTGT CCCCTGGGAT TCAGTACTTC CTGAAGGTGA CTGTCCTCGC TGGCCCATAC 
AGATCCTCGT CACACTTTGC CACTGAGTGG ACATATCCAC TGTCTCTGGC TAACGTGAGT 
GTACAGCCTG GCCGGAGACC TCAGGAGCTA CATGTGAGCT GGGTGGAGTC AGGTGGTGGC 
AGAGACCACT TGGTACAGCT CTCAGTGGCC GAGTCCCTAT CCATCATAAA GAATGTGTCT 
GTCCCCCGTG GAGTCAACCA GCTTGACCTG GAGGGGCTGG TTCCAGGGTC TCGATACCGT 
GTGGAGATCA TTTCTCAGGC TGGGCCTCAT CGCACCTCCT CTCAGACTGC CATTGGCTAC 
ACTGTCCCAC TACCTCCGCT TTCCCTCTCG GCAAGCCCTG TCAGCACTGC CTGGGCTCTG 
GCTGTGCACT GGGAGACCCC TTCTGGGCAG AGGGATGGAT ATCTGCTCAG TGTGCATGAG 
GAAGGTTCTT CTAAACCAGC AAGGAACCTG AAAGTAGGGA AAGACAGCAC CAATGTCACC 
CTGGCACAGC TGGAACCAGG AACTTGCTAC CTTGTTGAGA TCTGGGCTGT AGCTGGACCT 
TACCGCTCAC TCCCCAAGAA CTTCACTGGC TGCACAGTTC CTGCTGCACC GACAGACCTG 
AGCCTCACCA ACCCAGGCAG CTCCTCAGAG CTTTACACAT GCTGGAACAA GCCCCCCGGC 
AGGAGGGATC GCTACCATGT TATCCTGTAT GGCCTCAGCA TCCAGAGTAG GAATCGGGTC 
CAGACCTTGA GTCCAGATGC CCAGAACATC ACCTGGACTC ACCTGGAGGC AGGCAGCAGG 
TTTGCTGTGC AGGTCACTGC TGTGAAAGGT TCATTAGAAG CCTCCTCCAC CAACGTCACC 
CAATGGACAT ATCCCTTGGC CCCTGCCAAC CTCACCCTGG GCAGCCCTTC TGCCTCCGAG 
CTGCAGGCCT CCTGGGCAAC AACGGGGCGA GGAGCAGAAG GCTATGTGGT GGATGTCTAT 
GATAGAGCCT CCAGCAGTCA CGTTGGGCAC GTGGTGCTGG GTGGGGATGC CCGGAGTCAC 
ACCTTCAGAA AGCTGAGCCC TGGTACTCGC TACAGCGTGG CAGTGAGGGC CACAGCTGGG 
CCCTTCCACA CCAGCACCCC CAACTTCACC CTCTGCACAC GCCCGCTGCC CCCAGCCTCC 
GTGCGCTTGC TGAGCACGGG GCACCCCGAC AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT 
GGGGGGCGAG ACGGCTACAC ACTGACCCTG TACCACGGGC GGCTGGGCAG CATGGTGGCT 
ACCACCTCTC TTGGGAGAGA CACCCACAAC TTCACCTTCA CGGGCCTGAA CCCAGGAAGC 
AAGTATGTTC TGGAGGCGGG ATCCATAGCA GGGTCCTACC AGACACCTGC AGGGAACATC 
AGCAACTGGA CGTACCCCCT GGCACCTCGT AACGTGTACA TGACGAACCA GGGGTATCCC 
AACAGGCTGA GTGTGTCCTG GCGAGCTGAA CCCCAAGGGC AAGACAGTTA CAGGCTCCTC 
CTGTATCATT CGGGATCAGG TATTCTGACA GCCAATGTAT CTGTTGAGAA AGGCACCAGC 
AAATTCACCT TTTCTGGCCT GGCTCCGGGA CATAAATACC TGCTGGAAGT GGTGTCCACG 
GCAGGGCCCT ACGCAGCCTC TGCAGGGAAC ATCAGTGACT GGACAACCCC CTCAGTTCCC 
AAAAATCTCT CTGCGATGGC TGAAGGGAAC AACACAGTGC TCATCTCCTG GGACAGTGTC 
TCTGGACAAC AGGATGAGTG CCAGTTGTGG CTGCGGGATC CCAGGAACAG CACACTGCCC 
TGGCGGCACG CTCTCAGCCA AGGGCAAGTC CACCACCTCC TCCAGGGGCT GGTCCCAGGG 
AGGAACTACT CTGTCTCCTT GAGCTGCGTG GCCGGGCCCT ACTGGAGCAG CACCAAACCC 
TTGGCGGTGC TGATGGAGCC AAACCCAGTG GAAGATGTGC AATGCTTATC AGAGTCAAGG 
AGCCTTTACT TGAACTGGAC CAGCTCTCCT GGAGATGTGG AGGCTTATGA AGTGGTGACA 
GAGAGACTTT CTGATGGACT TCCCACCTCC AAGTATGTCA TGAGCATCCC TATGAGTGAG 
GCCAGCCTGG AGGGGCTGCA GCCAAACTCG TCATACCGAA TCGCTGTGAG CACAGTGGGC 
ATGAACACAA TTAGGAGCCA GGCTGTGACT CTGCTCTGCA ACACAACAGT GGAGGCCTTG 
CCTCCGCCCC TGCGAGCAGA CATCTTCCAA GTAGAGGCCA GCTCCGCAGT TATCATTTCA 
TCCGACCTGT TCAGTGAAGA GAATGGCCAG ATTGAGTATT ACGGTGTCGT TGCTACCACT 
AATGATTCAC TGCTGAGGCC CACCCAGGAA ATCATGTCCA GCACATGGTA TGACCACTAT 
TACGGGACAG AGGACTCCTA CCTGGCTGTG CTAATCCCCA ATCCTTTCCA TTCGAGCCCC 
AGGAGCTCCC CCGAAACCTG GCGAGTGCCA GTGGGAATAG AGGAGTGCGG CCAGTCCAGG 
GCAATGTGCA ACGGGAAGCT GAAAGCCAAC GAGCAGTACA GGTTCAGCAT TGCTGCCTTC 
ACCAAATACG ACCCAGTAGC CCCTGCAGTG ACCTTCACCA TGTTCTCAGC TGCTGAATCC 
AGTGCAGATG CAACTCCACT CTCAATGCCA ATAATCACTG GAATCATCGT GGGATTTCTC 
TTGACACTTG CAGCTATTTT TGCTTTGGTC TACTGGAAAC AGCTCAGAGC AAAGAGAACC 
AACAAAAGCA GCCTGCCCCA GGAAATGGTG ACCTACAGCT TGAGAAACAT CCATCGGCCA 
ATTCCCATAC AGAACTTCAA GCAGTACTAT GAGTTGAAGA CAGCAAGTGC TAACCACGCT 
TTTTTCCAGG AGTTTGAGGA GCTGAAGGAA GTTGGGAAGG AGCAGCCAAA GGTGGAGGCT 
GAGCTACCAG CAAATGTCTC CAAGAACAGA TATCCCCACG TGCTGCCCTA TGATCACTCT 
CGGGTCAAGC TGAGCCAACT GGGGGAGGAT CCACACTCTG ACTACATCAA TGCCAACTTC 
ATGCCTGGCT ATACATCCCA GCAGGAGTTC ATTGCCACCC AGGGGCCCCT GAAGAAAACG 
ATAGAGGACT TCTGGAGGCT GGTGTGGGAG CAGAATGTCT GCAACATCAT CATGCTGACA 
GTATGCATGG AGAACGGGCG GGTTCTCTGT GATCACTACT GGCCATCCGA ATCTGCCCCA 
GTCTCCTATG GGCACATCCG TGTCCACTTG CTGTCACAGA GCAGCTCCGA GGAGTGGACG 
ATGCGCGAGT TCAAACTGTG GCATGAGGGT ATCCGGGCAG AGCGGCATGT GTCCCACCTG 
CACTACACGG CATGGCCTGA CCATGGGATC CCAGAGTCCA CGACTTCCAT TATGACCTTC 
AGAGAGCTGG TCCGGGAGCG TATCCAGAGC ACCAAGGACG CGGGGCCAAC CCTCGTGCAC 
TGCAGCGCTG GGGTGGGCCG CACTGGCACC TTCATTGCAC TGGACCGGCT GCTGCAGCAG 
ATGAAGCAGG AGAAGGTGGT GGACACGTTT GGTGTTGTTT ACACCTTGCG GATGAACCGT 
TACCTGATGA TTCAGACGCT GTCCCAGTAT ATTTTCCTGC ACAGCTGTAT TCTGGAAAAG 
ATCTTAGAGG AACCACTCCT GGGTTTATCA GGGACAGAGA GGTCCTGCCC CATCCCGCTG 
AAAAGCTTTG CTCAGCACTA TGCCCAGAAG GCAACCAAGT CGCATGTGGG CTTCCTGAGG 
GAGTACGAGA CCCTCCTAGA GGCTGTCAAG GAAGAAGCCA GCTCTGCAAC ACCATCTTCA 
GGCGACCAGC AGACACATCC CTCTTCCAGT ATCCTCCCAT ATGATCGCTC CAGAGTGAAG 
TTTTCCCTGC TGGAACAGGG CCCATTCTCA GGTCTCCTGC AGGTGTGGCG TGTCCCGGGC 
TGCAGCTCCA GCAGGGAATA TCTGGCTGTC CAGGGGCCTG ACAAGTTGAC TGTGGAGGAC 
TTCTGGACCC TGGTGTGGGA ACAGGACGTT CACACCATCC TAACACTTCT CCCATGGCAG 
GAGAAGGGGG AGATCCCAAG TGGGGCGTGC TGGCCCCTGG AAGGAGACTC TCTCTGCACA 
AAGACGCTGA CCATCCAGTG TGGCACAGAG AAGCTTGTCT CGGGATGGAG GTGCATCCAG 
CTCAAAATGA AACATGAGAA GAAAGCAAAG GAGAGGCAGG TACAACAGTT CCTATACACA 
CTGTGGAGCA GCAAGAAACA GCCAGATGTC CAAAGCCTGG TGGAGTTCCT CACTGCTGTC 
AGACGGTGCA TGCCCCACCG AAAGAGAGCC GGTCCTCTCC TGCTGCACTG CAGCAGTGGC 
GTGAGCCAAA TGGGGACACT GATCTCCCTG GACTGCCTGT TGCACCAGAT GAAAGCTGAG 
AGAACGGTGG ATGTCTATGG GGTCACCCTC CAGCTGGCAA GAAGCTGTTG TCTCATGACC 
CCAACACTGG ACCAGTACAT GTTCCTGTAC ACCTGCATCC GGGACATCAT TGCTCAGAAA 
CAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801832.1
CDS	422..6970
Translation	MRPPPPLLLLVLWVLRLLGTLAEDEGCNQTARAGLEGQDAPNDRGTLLNLSVSDHGWSDSLLLSWDEPEGGAKGYSLALSSLGSGTPLQNGSAGPNITSFWFHGLTPGMRYEIEVTATLACMETTSQTVTAQTSPSPVRNLTLSSGGSSAMLQASWAHAHGQRDSYHLALYHSDSQTLVRNVSILPNASTFLFDGLLPGSEYALKVSTLVGSSQASTSIHQWTAPSIPTQLRLSPGSSTSLIASWADAGGAAWLHLTLHNLLTQMVTTTLSARRGLTSYTFQHLHSGTQYWLGLSAMAGPYTMVGPNATAWTYPLSPGNVTLSPAEEQNSLQARWSTPAGERDFYLVTLQEEEDGVPTRNISVDGDNSHVTFHGLDPGKQYSVQVTAVAGPYRASAHSTAAWTQPLAPSGVSLSSQGSPYSLLASWEEAMGEGYVLALSPMEHPVKNSSLVKGVTNFTYNGLHPGTLYTFEVSTVAGPYTSSPRRITNWTYPLPLEQLTLSNQGHSTSLQASWRAAPAGSTGYAGTLWETKSQERVRNMTVGNNWMNATFEDLVPGRQYTLEMTAMAGPYRSPVRSATDWTYPLAPAGVTLTNTRRPLGLAAFWDKAAGDVDQFHLQLYSKSHPAQRNISVGPDTHNFTFLGLSPGIQYFLKVTVLAGPYRSSSHFATEWTYPLSLANVSVQPGRRPQELHVSWVESGGGRDHLVQLSVAESLSIIKNVSVPRGVNQLDLEGLVPGSRYRVEIISQAGPHRTSSQTAIGYTVPLPPLSLSASPVSTAWALAVHWETPSGQRDGYLLSVHEEGSSKPARNLKVGKDSTNVTLAQLEPGTCYLVEIWAVAGPYRSLPKNFTGCTVPAAPTDLSLTNPGSSSELYTCWNKPPGRRDRYHVILYGLSIQSRNRVQTLSPDAQNITWTHLEAGSRFAVQVTAVKGSLEASSTNVTQWTYPLAPANLTLGSPSASELQASWATTGRGAEGYVVDVYDRASSSHVGHVVLGGDARSHTFRKLSPGTRYSVAVRATAGPFHTSTPNFTLCTRPLPPASVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFTGLNPGSKYVLEAGSIAGSYQTPAGNISNWTYPLAPRNVYMTNQGYPNRLSVSWRAEPQGQDSYRLLLYHSGSGILTANVSVEKGTSKFTFSGLAPGHKYLLEVVSTAGPYAASAGNISDWTTPSVPKNLSAMAEGNNTVLISWDSVSGQQDECQLWLRDPRNSTLPWRHALSQGQVHHLLQGLVPGRNYSVSLSCVAGPYWSSTKPLAVLMEPNPVEDVQCLSESRSLYLNWTSSPGDVEAYEVVTERLSDGLPTSKYVMSIPMSEASLEGLQPNSSYRIAVSTVGMNTIRSQAVTLLCNTTVEALPPPLRADIFQVEASSAVIISSDLFSEENGQIEYYGVVATTNDSLLRPTQEIMSSTWYDHYYGTEDSYLAVLIPNPFHSSPRSSPETWRVPVGIEECGQSRAMCNGKLKANEQYRFSIAAFTKYDPVAPAVTFTMFSAAESSADATPLSMPIITGIIVGFLLTLAAIFALVYWKQLRAKRTNKSSLPQEMVTYSLRNIHRPIPIQNFKQYYELKTASANHAFFQEFEELKEVGKEQPKVEAELPANVSKNRYPHVLPYDHSRVKLSQLGEDPHSDYINANFMPGYTSQQEFIATQGPLKKTIEDFWRLVWEQNVCNIIMLTVCMENGRVLCDHYWPSESAPVSYGHIRVHLLSQSSSEEWTMREFKLWHEGIRAERHVSHLHYTAWPDHGIPESTTSIMTFRELVRERIQSTKDAGPTLVHCSAGVGRTGTFIALDRLLQQMKQEKVVDTFGVVYTLRMNRYLMIQTLSQYIFLHSCILEKILEEPLLGLSGTERSCPIPLKSFAQHYAQKATKSHVGFLREYETLLEAVKEEASSATPSSGDQQTHPSSSILPYDRSRVKFSLLEQGPFSGLLQVWRVPGCSSSREYLAVQGPDKLTVEDFWTLVWEQDVHTILTLLPWQEKGEIPSGACWPLEGDSLCTKTLTIQCGTEKLVSGWRCIQLKMKHEKKAKERQVQQFLYTLWSSKKQPDVQSLVEFLTAVRRCMPHRKRAGPLLLHCSSGVSQMGTLISLDCLLHQMKAERTVDVYGVTLQLARSCCLMTPTLDQYMFLYTCIRDIIAQKQP

Target ORF information:

RefSeq Version	XM_014946346.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946346.1

ORF Insert Sequence:

ATGCGACCGC CGCCGCCGCT GCTGCTGCTC GTCCTGTGGG TGCTGCGGCT CCTGGGAACC 
CTGGCAGAGG ATGAGGGATG CAACCAGACA GCACGGGCAG GGTTGGAGGG ACAGGATGCA 
CCCAATGATA GAGGGACCCT CCTGAACCTC AGTGTGAGTG ACCATGGCTG GTCGGACTCC 
CTCCTTCTGT CCTGGGATGA GCCAGAGGGG GGTGCCAAAG GATATTCGCT CGCTCTCTCC 
TCCCTGGGGT CAGGCACACC GTTGCAGAAT GGATCTGCTG GACCCAACAT CACCAGCTTC 
TGGTTCCATG GGCTGACCCC TGGCATGCGC TATGAGATTG AGGTGACAGC CACACTTGCC 
TGCATGGAGA CCACCAGCCA GACAGTCACA GCACAGACAA GTCCTTCACC TGTCCGCAAC 
CTGACCCTGA GCAGTGGTGG GAGCTCTGCC ATGCTGCAGG CGTCCTGGGC GCATGCCCAC 
GGGCAGCGAG ACAGCTACCA CCTAGCCCTC TACCACAGCG ACTCCCAGAC GCTGGTGAGA 
AATGTGTCCA TCCTGCCAAA CGCCTCTACC TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT 
GAGTACGCCT TGAAGGTCAG CACGCTGGTT GGATCCAGTC AGGCGAGCAC CAGTATCCAC 
CAGTGGACAG CTCCCTCCAT CCCCACCCAG CTGAGACTCA GCCCAGGATC CAGCACCAGC 
CTCATCGCCT CCTGGGCAGA TGCTGGGGGG GCTGCCTGGC TGCACCTCAC ACTCCACAAC 
CTCCTCACCC AGATGGTGAC CACCACCCTG TCAGCCAGAA GGGGCCTCAC CAGCTACACC 
TTCCAGCATC TCCACTCCGG CACCCAGTAC TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC 
TACACCATGG TGGGGCCCAA TGCCACCGCT TGGACATACC CCCTGAGCCC TGGAAATGTG 
ACCCTGAGCC CTGCAGAGGA ACAGAACTCC TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA 
GAGAGGGACT TCTACCTGGT GACACTGCAG GAGGAAGAGG ATGGTGTGCC AACAAGGAAC 
ATCTCTGTCG ATGGAGACAA CAGTCATGTC ACTTTCCACG GGCTGGATCC TGGGAAGCAA 
TACTCTGTCC AGGTGACAGC AGTGGCTGGG CCTTACAGAG CATCAGCCCA CAGCACAGCA 
GCCTGGACAC AGCCGCTAGC ACCATCTGGT GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC 
AGCCTACTGG CCAGCTGGGA GGAGGCAATG GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC 
ATGGAGCACC CTGTGAAGAA TAGCTCGCTG GTTAAAGGTG TCACCAACTT CACCTACAAC 
GGCCTCCACC CTGGGACTCT TTACACCTTT GAGGTCAGCA CCGTGGCTGG CCCCTACACA 
TCCTCGCCCC GGCGCATCAC CAACTGGACA TATCCTTTGC CCCTGGAGCA GCTGACCCTC 
AGCAATCAGG GCCACAGCAC CTCTCTGCAA GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC 
ACTGGCTACG CAGGCACCCT CTGGGAAACC AAGTCCCAGG AGCGGGTCAG GAACATGACT 
GTGGGGAACA ACTGGATGAA TGCCACCTTT GAGGACCTGG TCCCTGGGCG GCAGTATACA 
CTGGAGATGA CTGCTATGGC CGGACCTTAC AGATCCCCTG TGCGATCAGC CACAGACTGG 
ACATACCCTC TGGCTCCAGC CGGCGTGACC CTGACCAACA CCCGACGCCC ACTGGGACTT 
GCTGCCTTCT GGGACAAGGC TGCTGGTGAT GTGGACCAGT TCCACCTCCA GCTCTACAGC 
AAGAGCCATC CAGCACAGAG GAACATCTCA GTAGGGCCAG ACACCCACAA CTTCACATTC 
CTAGGGCTGT CCCCTGGGAT TCAGTACTTC CTGAAGGTGA CTGTCCTCGC TGGCCCATAC 
AGATCCTCGT CACACTTTGC CACTGAGTGG ACATATCCAC TGTCTCTGGC TAACGTGAGT 
GTACAGCCTG GCCGGAGACC TCAGGAGCTA CATGTGAGCT GGGTGGAGTC AGGTGGTGGC 
AGAGACCACT TGGTACAGCT CTCAGTGGCC GAGTCCCTAT CCATCATAAA GAATGTGTCT 
GTCCCCCGTG GAGTCAACCA GCTTGACCTG GAGGGGCTGG TTCCAGGGTC TCGATACCGT 
GTGGAGATCA TTTCTCAGGC TGGGCCTCAT CGCACCTCCT CTCAGACTGC CATTGGCTAC 
ACTGTCCCAC TACCTCCGCT TTCCCTCTCG GCAAGCCCTG TCAGCACTGC CTGGGCTCTG 
GCTGTGCACT GGGAGACCCC TTCTGGGCAG AGGGATGGAT ATCTGCTCAG TGTGCATGAG 
GAAGGTTCTT CTAAACCAGC AAGGAACCTG AAAGTAGGGA AAGACAGCAC CAATGTCACC 
CTGGCACAGC TGGAACCAGG AACTTGCTAC CTTGTTGAGA TCTGGGCTGT AGCTGGACCT 
TACCGCTCAC TCCCCAAGAA CTTCACTGGC TGCACAGTTC CTGCTGCACC GACAGACCTG 
AGCCTCACCA ACCCAGGCAG CTCCTCAGAG CTTTACACAT GCTGGAACAA GCCCCCCGGC 
AGGAGGGATC GCTACCATGT TATCCTGTAT GGCCTCAGCA TCCAGAGTAG GAATCGGGTC 
CAGACCTTGA GTCCAGATGC CCAGAACATC ACCTGGACTC ACCTGGAGGC AGGCAGCAGG 
TTTGCTGTGC AGGTCACTGC TGTGAAAGGT TCATTAGAAG CCTCCTCCAC CAACGTCACC 
CAATGGACAT ATCCCTTGGC CCCTGCCAAC CTCACCCTGG GCAGCCCTTC TGCCTCCGAG 
CTGCAGGCCT CCTGGGCAAC AACGGGGCGA GGAGCAGAAG GCTATGTGGT GGATGTCTAT 
GATAGAGCCT CCAGCAGTCA CGTTGGGCAC GTGGTGCTGG GTGGGGATGC CCGGAGTCAC 
ACCTTCAGAA AGCTGAGCCC TGGTACTCGC TACAGCGTGG CAGTGAGGGC CACAGCTGGG 
CCCTTCCACA CCAGCACCCC CAACTTCACC CTCTGCACAC GCCCGCTGCC CCCAGCCTCC 
GTGCGCTTGC TGAGCACGGG GCACCCCGAC AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT 
GGGGGGCGAG ACGGCTACAC ACTGACCCTG TACCACGGGC GGCTGGGCAG CATGGTGGCT 
ACCACCTCTC TTGGGAGAGA CACCCACAAC TTCACCTTCA CGGGCCTGAA CCCAGGAAGC 
AAGTATGTTC TGGAGGCGGG ATCCATAGCA GGGTCCTACC AGACACCTGC AGGGAACATC 
AGCAACTGGA CGTACCCCCT GGCACCTCGT AACGTGTACA TGACGAACCA GGGGTATCCC 
AACAGGCTGA GTGTGTCCTG GCGAGCTGAA CCCCAAGGGC AAGACAGTTA CAGGCTCCTC 
CTGTATCATT CGGGATCAGG TATTCTGACA GCCAATGTAT CTGTTGAGAA AGGCACCAGC 
AAATTCACCT TTTCTGGCCT GGCTCCGGGA CATAAATACC TGCTGGAAGT GGTGTCCACG 
GCAGGGCCCT ACGCAGCCTC TGCAGGGAAC ATCAGTGACT GGACAACCCC CTCAGTTCCC 
AAAAATCTCT CTGCGATGGC TGAAGGGAAC AACACAGTGC TCATCTCCTG GGACAGTGTC 
TCTGGACAAC AGGATGAGTG CCAGTTGTGG CTGCGGGATC CCAGGAACAG CACACTGCCC 
TGGCGGCACG CTCTCAGCCA AGGGCAAGTC CACCACCTCC TCCAGGGGCT GGTCCCAGGG 
AGGAACTACT CTGTCTCCTT GAGCTGCGTG GCCGGGCCCT ACTGGAGCAG CACCAAACCC 
TTGGCGGTGC TGATGGAGCC AAACCCAGTG GAAGATGTGC AATGCTTATC AGAGTCAAGG 
AGCCTTTACT TGAACTGGAC CAGCTCTCCT GGAGATGTGG AGGCTTATGA AGTGGTGACA 
GAGAGACTTT CTGATGGACT TCCCACCTCC AAGTATGTCA TGAGCATCCC TATGAGTGAG 
GCCAGCCTGG AGGGGCTGCA GCCAAACTCG TCATACCGAA TCGCTGTGAG CACAGTGGGC 
ATGAACACAA TTAGGAGCCA GGCTGTGACT CTGCTCTGCA ACACAACAGT GGAGGCCTTG 
CCTCCGCCCC TGCGAGCAGA CATCTTCCAA GTAGAGGCCA GCTCCGCAGT TATCATTTCA 
TCCGACCTGT TCAGTGAAGA GAATGGCCAG ATTGAGTATT ACGGTGTCGT TGCTACCACT 
AATGATTCAC TGCTGAGGCC CACCCAGGAA ATCATGTCCA GCACATGGTA TGACCACTAT 
TACGGGACAG AGGACTCCTA CCTGGCTGTG CTAATCCCCA ATCCTTTCCA TTCGAGCCCC 
AGGAGCTCCC CCGAAACCTG GCGAGTGCCA GTGGGAATAG AGGAGTGCGG CCAGTCCAGG 
GCAATGTGCA ACGGGAAGCT GAAAGCCAAC GAGCAGTACA GGTTCAGCAT TGCTGCCTTC 
ACCAAATACG ACCCAGTAGC CCCTGCAGTG ACCTTCACCA TGTTCTCAGC TGCTGAATCC 
AGTGCAGATG CAACTCCACT CTCAATGCCA ATAATCACTG GAATCATCGT GGGATTTCTC 
TTGACACTTG CAGCTATTTT TGCTTTGGTC TACTGGAAAC AGCTCAGAGC AAAGAGAACC 
AACAAAAGCA GCCTGCCCCA GGAAATGGTG ACCTACAGCT TGAGAAACAT CCATCGGCCA 
ATTCCCATAC AGAACTTCAA GCAGTACTAT GAGTTGAAGA CAGCAAGTGC TAACCACGCT 
TTTTTCCAGG AGTTTGAGGA GCTGAAGGAA GTTGGGAAGG AGCAGCCAAA GGTGGAGGCT 
GAGCTACCAG CAAATGTCTC CAAGAACAGA TATCCCCACG TGCTGCCCTA TGATCACTCT 
CGGGTCAAGC TGAGCCAACT GGGGGAGGAT CCACACTCTG ACTACATCAA TGCCAACTTC 
ATGCCTGGCT ATACATCCCA GCAGGAGTTC ATTGCCACCC AGGGGCCCCT GAAGAAAACG 
ATAGAGGACT TCTGGAGGCT GGTGTGGGAG CAGAATGTCT GCAACATCAT CATGCTGACA 
GTATGCATGG AGAACGGGCG GGTTCTCTGT GATCACTACT GGCCATCCGA ATCTGCCCCA 
GTCTCCTATG GGCACATCCG TGTCCACTTG CTGTCACAGA GCAGCTCCGA GGAGTGGACG 
ATGCGCGAGT TCAAACTGTG GCATGAGGGT ATCCGGGCAG AGCGGCATGT GTCCCACCTG 
CACTACACGG CATGGCCTGA CCATGGGATC CCAGAGTCCA CGACTTCCAT TATGACCTTC 
AGAGAGCTGG TCCGGGAGCG TATCCAGAGC ACCAAGGACG CGGGGCCAAC CCTCGTGCAC 
TGCAGCGCTG GGGTGGGCCG CACTGGCACC TTCATTGCAC TGGACCGGCT GCTGCAGCAG 
ATGAAGCAGG AGAAGGTGGT GGACACGTTT GGTGTTGTTT ACACCTTGCG GATGAACCGT 
TACCTGATGA TTCAGACGCT GTCCCAGTAT ATTTTCCTGC ACAGCTGTAT TCTGGAAAAG 
ATCTTAGAGG AACCACTCCT GGGTTTATCA GGGACAGAGA GGTCCTGCCC CATCCCGCTG 
AAAAGCTTTG CTCAGCACTA TGCCCAGAAG GCAACCAAGT CGCATGTGGG CTTCCTGAGG 
GAGTACGAGA CCCTCCTAGA GGCTGTCAAG GAAGAAGCCA GCTCTGCAAC ACCATCTTCA 
GGCGACCAGC AGACACATCC CTCTTCCAGT ATCCTCCCAT ATGATCGCTC CAGAGTGAAG 
TTTTCCCTGC TGGAACAGGG CCCATTCTCA GGTCTCCTGC AGGTGTGGCG TGTCCCGGGC 
TGCAGCTCCA GCAGGGAATA TCTGGCTGTC CAGGGGCCTG ACAAGTTGAC TGTGGAGGAC 
TTCTGGACCC TGGTGTGGGA ACAGGACGTT CACACCATCC TAACACTTCT CCCATGGCAG 
GAGAAGGGGG AGATCCCAAG TGGGGCGTGC TGGCCCCTGG AAGGAGACTC TCTCTGCACA 
AAGACGCTGA CCATCCAGTG TGGCACAGAG AAGCTTGTCT CGGGATGGAG GTGCATCCAG 
CTCAAAATGA AACATGAGAA GAAAGCAAAG GAGAGGCAGG TACAACAGTT CCTATACACA 
CTGTGGAGCA GCAAGAAACA GCCAGATGTC CAAAGCCTGG TGGAGTTCCT CACTGCTGTC 
AGACGGTGCA TGCCCCACCG AAAGAGAGCC GGTCCTCTCC TGCTGCACTG CAGCAGTGGC 
GTGAGCCAAA TGGGGACACT GATCTCCCTG GACTGCCTGT TGCACCAGAT GAAAGCTGAG 
AGAACGGTGG ATGTCTATGG GGTCACCCTC CAGCTGGCAA GAAGCTGTTG TCTCATGACC 
CCAACACTGG ACCAGTACAT GTTCCTGTAC ACCTGCATCC GGGACATCAT TGCTCAGAAA 
CAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106890484 ( XM_014946343.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801829.1 Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X1, mRNA.

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CloneID	OCa68219
Clone ID Related Accession (Same CDS sequence)	XM_014946343.1
Accession Version	XM_014946343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	receptor-type tyrosine-protein phosphatase V-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACCGC CGCCGCCGCT GCTGCTGCTC GTCCTGTGGG TGCTGCGGCT CCTGGGAACC 
CTGGCAGAGG ATGAGGGATG CAACCAGACA GCACGGGCAG GGTTGGAGGG ACAGGATGCA 
CCCAATGATA GAGGGACCCT CCTGAACCTC AGTGTGAGTG ACCATGGCTG GTCGGACTCC 
CTCCTTCTGT CCTGGGATGA GCCAGAGGGG GGTGCCAAAG GATATTCGCT CGCTCTCTCC 
TCCCTGGGGT CAGGCACACC GTTGCAGAAT GGATCTGCTG GACCCAACAT CACCAGCTTC 
TGGTTCCATG GGCTGACCCC TGGCATGCGC TATGAGATTG AGGTGACAGC CACACTTGCC 
TGCATGGAGA CCACCAGCCA GACAGTCACA GCACAGACAA GTCCTTCACC TGTCCGCAAC 
CTGACCCTGA GCAGTGGTGG GAGCTCTGCC ATGCTGCAGG CGTCCTGGGC GCATGCCCAC 
GGGCAGCGAG ACAGCTACCA CCTAGCCCTC TACCACAGCG ACTCCCAGAC GCTGGTGAGA 
AATGTGTCCA TCCTGCCAAA CGCCTCTACC TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT 
GAGTACGCCT TGAAGGTCAG CACGCTGGTT GGATCCAGTC AGGCGAGCAC CAGTATCCAC 
CAGTGGACAG CTCCCTCCAT CCCCACCCAG CTGAGACTCA GCCCAGGATC CAGCACCAGC 
CTCATCGCCT CCTGGGCAGA TGCTGGGGGG GCTGCCTGGC TGCACCTCAC ACTCCACAAC 
CTCCTCACCC AGATGGTGAC CACCACCCTG TCAGCCAGAA GGGGCCTCAC CAGCTACACC 
TTCCAGCATC TCCACTCCGG CACCCAGTAC TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC 
TACACCATGG TGGGGCCCAA TGCCACCGCT TGGACATACC CCCTGAGCCC TGGAAATGTG 
ACCCTGAGCC CTGCAGAGGA ACAGAACTCC TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA 
GAGAGGGACT TCTACCTGGT GACACTGCAG GAGGAAGAGG ATGGTGTGCC AACAAGGAAC 
ATCTCTGTCG ATGGAGACAA CAGTCATGTC ACTTTCCACG GGCTGGATCC TGGGAAGCAA 
TACTCTGTCC AGGTGACAGC AGTGGCTGGG CCTTACAGAG CATCAGCCCA CAGCACAGCA 
GCCTGGACAC AGCCGCTAGC ACCATCTGGT GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC 
AGCCTACTGG CCAGCTGGGA GGAGGCAATG GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC 
ATGGAGCACC CTGTGAAGAA TAGCTCGCTG GTTAAAGGTG TCACCAACTT CACCTACAAC 
GGCCTCCACC CTGGGACTCT TTACACCTTT GAGGTCAGCA CCGTGGCTGG CCCCTACACA 
TCCTCGCCCC GGCGCATCAC CAACTGGACA TATCCTTTGC CCCTGGAGCA GCTGACCCTC 
AGCAATCAGG GCCACAGCAC CTCTCTGCAA GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC 
ACTGGCTACG CAGGCACCCT CTGGGAAACC AAGTCCCAGG AGCGGGTCAG GAACATGACT 
GTGGGGAACA ACTGGATGAA TGCCACCTTT GAGGACCTGG TCCCTGGGCG GCAGTATACA 
CTGGAGATGA CTGCTATGGC CGGACCTTAC AGATCCCCTG TGCGATCAGC CACAGACTGG 
ACATACCCTC TGGCTCCAGC CGGCGTGACC CTGACCAACA CCCGACGCCC ACTGGGACTT 
GCTGCCTTCT GGGACAAGGC TGCTGGTGAT GTGGACCAGT TCCACCTCCA GCTCTACAGC 
AAGAGCCATC CAGCACAGAG GAACATCTCA GTAGGGCCAG ACACCCACAA CTTCACATTC 
CTAGGGCTGT CCCCTGGGAT TCAGTACTTC CTGAAGGTGA CTGTCCTCGC TGGCCCATAC 
AGATCCTCGT CACACTTTGC CACTGAGTGG ACATATCCAC TGTCTCTGGC TAACGTGAGT 
GTACAGCCTG GCCGGAGACC TCAGGAGCTA CATGTGAGCT GGGTGGAGTC AGGTGGTGGC 
AGAGACCACT TGGTACAGCT CTCAGTGGCC GAGTCCCTAT CCATCATAAA GAATGTGTCT 
GTCCCCCGTG GAGTCAACCA GCTTGACCTG GAGGGGCTGG TTCCAGGGTC TCGATACCGT 
GTGGAGATCA TTTCTCAGGC TGGGCCTCAT CGCACCTCCT CTCAGACTGC CATTGGCTAC 
ACTGTCCCAC TACCTCCGCT TTCCCTCTCG GCAAGCCCTG TCAGCACTGC CTGGGCTCTG 
GCTGTGCACT GGGAGACCCC TTCTGGGCAG AGGGATGGAT ATCTGCTCAG TGTGCATGAG 
GAAGGTTCTT CTAAACCAGC AAGGAACCTG AAAGTAGGGA AAGACAGCAC CAATGTCACC 
CTGGCACAGC TGGAACCAGG AACTTGCTAC CTTGTTGAGA TCTGGGCTGT AGCTGGACCT 
TACCGCTCAC TCCCCAAGAA CTTCACTGGC TGCACAGTTC CTGCTGCACC GACAGACCTG 
AGCCTCACCA ACCCAGGCAG CTCCTCAGAG CTTTACACAT GCTGGAACAA GCCCCCCGGC 
AGGAGGGATC GCTACCATGT TATCCTGTAT GGCCTCAGCA TCCAGAGTAG GAATCGGGTC 
CAGACCTTGA GTCCAGATGC CCAGAACATC ACCTGGACTC ACCTGGAGGC AGGCAGCAGG 
TTTGCTGTGC AGGTCACTGC TGTGAAAGGT TCATTAGAAG CCTCCTCCAC CAACGTCACC 
CAATGGACAT ATCCCTTGGC CCCTGCCAAC CTCACCCTGG GCAGCCCTTC TGCCTCCGAG 
CTGCAGGCCT CCTGGGCAAC AACGGGGCGA GGAGCAGAAG GCTATGTGGT GGATGTCTAT 
GATAGAGCCT CCAGCAGTCA CGTTGGGCAC GTGGTGCTGG GTGGGGATGC CCGGAGTCAC 
ACCTTCAGAA AGCTGAGCCC TGGTACTCGC TACAGCGTGG CAGTGAGGGC CACAGCTGGG 
CCCTTCCACA CCAGCACCCC CAACTTCACC CTCTGCACAC GCCCGCTGCC CCCAGCCGCC 
GTGCGCTTGC TGAGCACGGG GCACCCCGAC AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT 
GGGGGGCGAG ACGGCTACAC ACTGACCCTG TACCACGGGC GGCTGGGCAG CATGGTGGCT 
ACCACCTCTC TTGGGAGAGA CACCCACAAC TTCACCTTCA TGGGTCTGAA CCCAGGATAC 
AAGTACTCCC TGGAGGCCAG TGCCATGGCT GGACCATACC ACGCAGCAGC ACCCAACATC 
AGTGCCTGGA CACGCCCGCT GCCCCCAGCC TCCGTGCGCT TGCTGAGCAC GGGGCACCCC 
GACAGGCTGA GTGCGTCCTG GGCAGCCGCG GCTGGGGGGC GAGACGGCTA CACACTGACC 
CTGTACCACG GGCGGCTGGG CAGCATGGTG GCTACCACCT CTCTTGGGAG AGACACCCAC 
AACTTCACCT TCACGGGCCT GAACCCAGGA AGCAAGTATG TTCTGGAGGC GGGATCCATA 
GCAGGGTCCT ACCAGACACC TGCAGGGAAC ATCAGCAACT GGACGTACCC CCTGGCACCT 
CGTAACGTGT ACATGACGAA CCAGGGGTAT CCCAACAGGC TGAGTGTGTC CTGGCGAGCT 
GAACCCCAAG GGCAAGACAG TTACAGGCTC CTCCTGTATC ATTCGGGATC AGGTATTCTG 
ACAGCCAATG TATCTGTTGA GAAAGGCACC AGCAAATTCA CCTTTTCTGG CCTGGCTCCG 
GGACATAAAT ACCTGCTGGA AGTGGTGTCC ACGGCAGGGC CCTACGCAGC CTCTGCAGGG 
AACATCAGTG ACTGGACAAC CCCCTCAGTT CCCAAAAATC TCTCTGCGAT GGCTGAAGGG 
AACAACACAG TGCTCATCTC CTGGGACAGT GTCTCTGGAC AACAGGATGA GTGCCAGTTG 
TGGCTGCGGG ATCCCAGGAA CAGCACACTG CCCTGGCGGC ACGCTCTCAG CCAAGGGCAA 
GTCCACCACC TCCTCCAGGG GCTGGTCCCA GGGAGGAACT ACTCTGTCTC CTTGAGCTGC 
GTGGCCGGGC CCTACTGGAG CAGCACCAAA CCCTTGGCGG TGCTGATGGA GCCAAACCCA 
GTGGAAGATG TGCAATGCTT ATCAGAGTCA AGGAGCCTTT ACTTGAACTG GACCAGCTCT 
CCTGGAGATG TGGAGGCTTA TGAAGTGGTG ACAGAGAGAC TTTCTGATGG ACTTCCCACC 
TCCAAGTATG TCATGAGCAT CCCTATGAGT GAGGCCAGCC TGGAGGGGCT GCAGCCAAAC 
TCGTCATACC GAATCGCTGT GAGCACAGTG GGCATGAACA CAATTAGGAG CCAGGCTGTG 
ACTCTGCTCT GCAACACAAC AGTGGAGGCC TTGCCTCCGC CCCTGCGAGC AGACATCTTC 
CAAGTAGAGG CCAGCTCCGC AGTTATCATT TCATCCGACC TGTTCAGTGA AGAGAATGGC 
CAGATTGAGT ATTACGGTGT CGTTGCTACC ACTAATGATT CACTGCTGAG GCCCACCCAG 
GAAATCATGT CCAGCACATG GTATGACCAC TATTACGGGA CAGAGGACTC CTACCTGGCT 
GTGCTAATCC CCAATCCTTT CCATTCGAGC CCCAGGAGCT CCCCCGAAAC CTGGCGAGTG 
CCAGTGGGAA TAGAGGAGTG CGGCCAGTCC AGGGCAATGT GCAACGGGAA GCTGAAAGCC 
AACGAGCAGT ACAGGTTCAG CATTGCTGCC TTCACCAAAT ACGACCCAGT AGCCCCTGCA 
GTGACCTTCA CCATGTTCTC AGCTGCTGAA TCCAGTGCAG ATGCAACTCC ACTCTCAATG 
CCAATAATCA CTGGAATCAT CGTGGGATTT CTCTTGACAC TTGCAGCTAT TTTTGCTTTG 
GTCTACTGGA AACAGCTCAG AGCAAAGAGA ACCAACAAAA GCAGCCTGCC CCAGGAAATG 
GTGACCTACA GCTTGAGAAA CATCCATCGG CCAATTCCCA TACAGAACTT CAAGCAGTAC 
TATGAGTTGA AGACAGCAAG TGCTAACCAC GCTTTTTTCC AGGAGTTTGA GGAGCTGAAG 
GAAGTTGGGA AGGAGCAGCC AAAGGTGGAG GCTGAGCTAC CAGCAAATGT CTCCAAGAAC 
AGATATCCCC ACGTGCTGCC CTATGATCAC TCTCGGGTCA AGCTGAGCCA ACTGGGGGAG 
GATCCACACT CTGACTACAT CAATGCCAAC TTCATGCCTG GCTATACATC CCAGCAGGAG 
TTCATTGCCA CCCAGGGGCC CCTGAAGAAA ACGATAGAGG ACTTCTGGAG GCTGGTGTGG 
GAGCAGAATG TCTGCAACAT CATCATGCTG ACAGTATGCA TGGAGAACGG GCGGGTTCTC 
TGTGATCACT ACTGGCCATC CGAATCTGCC CCAGTCTCCT ATGGGCACAT CCGTGTCCAC 
TTGCTGTCAC AGAGCAGCTC CGAGGAGTGG ACGATGCGCG AGTTCAAACT GTGGCATGAG 
GGTATCCGGG CAGAGCGGCA TGTGTCCCAC CTGCACTACA CGGCATGGCC TGACCATGGG 
ATCCCAGAGT CCACGACTTC CATTATGACC TTCAGAGAGC TGGTCCGGGA GCGTATCCAG 
AGCACCAAGG ACGCGGGGCC AACCCTCGTG CACTGCAGCG CTGGGGTGGG CCGCACTGGC 
ACCTTCATTG CACTGGACCG GCTGCTGCAG CAGATGAAGC AGGAGAAGGT GGTGGACACG 
TTTGGTGTTG TTTACACCTT GCGGATGAAC CGTTACCTGA TGATTCAGAC GCTGTCCCAG 
TATATTTTCC TGCACAGCTG TATTCTGGAA AAGATCTTAG AGGAACCACT CCTGGGTTTA 
TCAGGGACAG AGAGGTCCTG CCCCATCCCG CTGAAAAGCT TTGCTCAGCA CTATGCCCAG 
AAGGCAACCA AGTCGCATGT GGGCTTCCTG AGGGAGTACG AGACCCTCCT AGAGGCTGTC 
AAGGAAGAAG CCAGCTCTGC AACACCATCT TCAGGCGACC AGCAGACACA TCCCTCTTCC 
AGTATCCTCC CATATGATCG CTCCAGAGTG AAGTTTTCCC TGCTGGAACA GGGCCCATTC 
TCAGGTCTCC TGCAGGTGTG GCGTGTCCCG GGCTGCAGCT CCAGCAGGGA ATATCTGGCT 
GTCCAGGGGC CTGACAAGTT GACTGTGGAG GACTTCTGGA CCCTGGTGTG GGAACAGGAC 
GTTCACACCA TCCTAACACT TCTCCCATGG CAGGAGAAGG GGGAGATCCC AAGTGGGGCG 
TGCTGGCCCC TGGAAGGAGA CTCTCTCTGC ACAAAGACGC TGACCATCCA GTGTGGCACA 
GAGAAGCTTG TCTCGGGATG GAGGTGCATC CAGCTCAAAA TGAAACATGA GAAGAAAGCA 
AAGGAGAGGC AGGTACAACA GTTCCTATAC ACACTGTGGA GCAGCAAGAA ACAGCCAGAT 
GTCCAAAGCC TGGTGGAGTT CCTCACTGCT GTCAGACGGT GCATGCCCCA CCGAAAGAGA 
GCCGGTCCTC TCCTGCTGCA CTGCAGCAGT GGCGTGAGCC AAATGGGGAC ACTGATCTCC 
CTGGACTGCC TGTTGCACCA GATGAAAGCT GAGAGAACGG TGGATGTCTA TGGGGTCACC 
CTCCAGCTGG CAAGAAGCTG TTGTCTCATG ACCCCAACAC TGGACCAGTA CATGTTCCTG 
TACACCTGCA TCCGGGACAT CATTGCTCAG AAACAGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801829.1
CDS	422..7243
Translation	MRPPPPLLLLVLWVLRLLGTLAEDEGCNQTARAGLEGQDAPNDRGTLLNLSVSDHGWSDSLLLSWDEPEGGAKGYSLALSSLGSGTPLQNGSAGPNITSFWFHGLTPGMRYEIEVTATLACMETTSQTVTAQTSPSPVRNLTLSSGGSSAMLQASWAHAHGQRDSYHLALYHSDSQTLVRNVSILPNASTFLFDGLLPGSEYALKVSTLVGSSQASTSIHQWTAPSIPTQLRLSPGSSTSLIASWADAGGAAWLHLTLHNLLTQMVTTTLSARRGLTSYTFQHLHSGTQYWLGLSAMAGPYTMVGPNATAWTYPLSPGNVTLSPAEEQNSLQARWSTPAGERDFYLVTLQEEEDGVPTRNISVDGDNSHVTFHGLDPGKQYSVQVTAVAGPYRASAHSTAAWTQPLAPSGVSLSSQGSPYSLLASWEEAMGEGYVLALSPMEHPVKNSSLVKGVTNFTYNGLHPGTLYTFEVSTVAGPYTSSPRRITNWTYPLPLEQLTLSNQGHSTSLQASWRAAPAGSTGYAGTLWETKSQERVRNMTVGNNWMNATFEDLVPGRQYTLEMTAMAGPYRSPVRSATDWTYPLAPAGVTLTNTRRPLGLAAFWDKAAGDVDQFHLQLYSKSHPAQRNISVGPDTHNFTFLGLSPGIQYFLKVTVLAGPYRSSSHFATEWTYPLSLANVSVQPGRRPQELHVSWVESGGGRDHLVQLSVAESLSIIKNVSVPRGVNQLDLEGLVPGSRYRVEIISQAGPHRTSSQTAIGYTVPLPPLSLSASPVSTAWALAVHWETPSGQRDGYLLSVHEEGSSKPARNLKVGKDSTNVTLAQLEPGTCYLVEIWAVAGPYRSLPKNFTGCTVPAAPTDLSLTNPGSSSELYTCWNKPPGRRDRYHVILYGLSIQSRNRVQTLSPDAQNITWTHLEAGSRFAVQVTAVKGSLEASSTNVTQWTYPLAPANLTLGSPSASELQASWATTGRGAEGYVVDVYDRASSSHVGHVVLGGDARSHTFRKLSPGTRYSVAVRATAGPFHTSTPNFTLCTRPLPPAAVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFMGLNPGYKYSLEASAMAGPYHAAAPNISAWTRPLPPASVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFTGLNPGSKYVLEAGSIAGSYQTPAGNISNWTYPLAPRNVYMTNQGYPNRLSVSWRAEPQGQDSYRLLLYHSGSGILTANVSVEKGTSKFTFSGLAPGHKYLLEVVSTAGPYAASAGNISDWTTPSVPKNLSAMAEGNNTVLISWDSVSGQQDECQLWLRDPRNSTLPWRHALSQGQVHHLLQGLVPGRNYSVSLSCVAGPYWSSTKPLAVLMEPNPVEDVQCLSESRSLYLNWTSSPGDVEAYEVVTERLSDGLPTSKYVMSIPMSEASLEGLQPNSSYRIAVSTVGMNTIRSQAVTLLCNTTVEALPPPLRADIFQVEASSAVIISSDLFSEENGQIEYYGVVATTNDSLLRPTQEIMSSTWYDHYYGTEDSYLAVLIPNPFHSSPRSSPETWRVPVGIEECGQSRAMCNGKLKANEQYRFSIAAFTKYDPVAPAVTFTMFSAAESSADATPLSMPIITGIIVGFLLTLAAIFALVYWKQLRAKRTNKSSLPQEMVTYSLRNIHRPIPIQNFKQYYELKTASANHAFFQEFEELKEVGKEQPKVEAELPANVSKNRYPHVLPYDHSRVKLSQLGEDPHSDYINANFMPGYTSQQEFIATQGPLKKTIEDFWRLVWEQNVCNIIMLTVCMENGRVLCDHYWPSESAPVSYGHIRVHLLSQSSSEEWTMREFKLWHEGIRAERHVSHLHYTAWPDHGIPESTTSIMTFRELVRERIQSTKDAGPTLVHCSAGVGRTGTFIALDRLLQQMKQEKVVDTFGVVYTLRMNRYLMIQTLSQYIFLHSCILEKILEEPLLGLSGTERSCPIPLKSFAQHYAQKATKSHVGFLREYETLLEAVKEEASSATPSSGDQQTHPSSSILPYDRSRVKFSLLEQGPFSGLLQVWRVPGCSSSREYLAVQGPDKLTVEDFWTLVWEQDVHTILTLLPWQEKGEIPSGACWPLEGDSLCTKTLTIQCGTEKLVSGWRCIQLKMKHEKKAKERQVQQFLYTLWSSKKQPDVQSLVEFLTAVRRCMPHRKRAGPLLLHCSSGVSQMGTLISLDCLLHQMKAERTVDVYGVTLQLARSCCLMTPTLDQYMFLYTCIRDIIAQKQP

Target ORF information:

RefSeq Version	XM_014946343.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946343.1

ORF Insert Sequence:

ATGCGACCGC CGCCGCCGCT GCTGCTGCTC GTCCTGTGGG TGCTGCGGCT CCTGGGAACC 
CTGGCAGAGG ATGAGGGATG CAACCAGACA GCACGGGCAG GGTTGGAGGG ACAGGATGCA 
CCCAATGATA GAGGGACCCT CCTGAACCTC AGTGTGAGTG ACCATGGCTG GTCGGACTCC 
CTCCTTCTGT CCTGGGATGA GCCAGAGGGG GGTGCCAAAG GATATTCGCT CGCTCTCTCC 
TCCCTGGGGT CAGGCACACC GTTGCAGAAT GGATCTGCTG GACCCAACAT CACCAGCTTC 
TGGTTCCATG GGCTGACCCC TGGCATGCGC TATGAGATTG AGGTGACAGC CACACTTGCC 
TGCATGGAGA CCACCAGCCA GACAGTCACA GCACAGACAA GTCCTTCACC TGTCCGCAAC 
CTGACCCTGA GCAGTGGTGG GAGCTCTGCC ATGCTGCAGG CGTCCTGGGC GCATGCCCAC 
GGGCAGCGAG ACAGCTACCA CCTAGCCCTC TACCACAGCG ACTCCCAGAC GCTGGTGAGA 
AATGTGTCCA TCCTGCCAAA CGCCTCTACC TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT 
GAGTACGCCT TGAAGGTCAG CACGCTGGTT GGATCCAGTC AGGCGAGCAC CAGTATCCAC 
CAGTGGACAG CTCCCTCCAT CCCCACCCAG CTGAGACTCA GCCCAGGATC CAGCACCAGC 
CTCATCGCCT CCTGGGCAGA TGCTGGGGGG GCTGCCTGGC TGCACCTCAC ACTCCACAAC 
CTCCTCACCC AGATGGTGAC CACCACCCTG TCAGCCAGAA GGGGCCTCAC CAGCTACACC 
TTCCAGCATC TCCACTCCGG CACCCAGTAC TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC 
TACACCATGG TGGGGCCCAA TGCCACCGCT TGGACATACC CCCTGAGCCC TGGAAATGTG 
ACCCTGAGCC CTGCAGAGGA ACAGAACTCC TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA 
GAGAGGGACT TCTACCTGGT GACACTGCAG GAGGAAGAGG ATGGTGTGCC AACAAGGAAC 
ATCTCTGTCG ATGGAGACAA CAGTCATGTC ACTTTCCACG GGCTGGATCC TGGGAAGCAA 
TACTCTGTCC AGGTGACAGC AGTGGCTGGG CCTTACAGAG CATCAGCCCA CAGCACAGCA 
GCCTGGACAC AGCCGCTAGC ACCATCTGGT GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC 
AGCCTACTGG CCAGCTGGGA GGAGGCAATG GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC 
ATGGAGCACC CTGTGAAGAA TAGCTCGCTG GTTAAAGGTG TCACCAACTT CACCTACAAC 
GGCCTCCACC CTGGGACTCT TTACACCTTT GAGGTCAGCA CCGTGGCTGG CCCCTACACA 
TCCTCGCCCC GGCGCATCAC CAACTGGACA TATCCTTTGC CCCTGGAGCA GCTGACCCTC 
AGCAATCAGG GCCACAGCAC CTCTCTGCAA GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC 
ACTGGCTACG CAGGCACCCT CTGGGAAACC AAGTCCCAGG AGCGGGTCAG GAACATGACT 
GTGGGGAACA ACTGGATGAA TGCCACCTTT GAGGACCTGG TCCCTGGGCG GCAGTATACA 
CTGGAGATGA CTGCTATGGC CGGACCTTAC AGATCCCCTG TGCGATCAGC CACAGACTGG 
ACATACCCTC TGGCTCCAGC CGGCGTGACC CTGACCAACA CCCGACGCCC ACTGGGACTT 
GCTGCCTTCT GGGACAAGGC TGCTGGTGAT GTGGACCAGT TCCACCTCCA GCTCTACAGC 
AAGAGCCATC CAGCACAGAG GAACATCTCA GTAGGGCCAG ACACCCACAA CTTCACATTC 
CTAGGGCTGT CCCCTGGGAT TCAGTACTTC CTGAAGGTGA CTGTCCTCGC TGGCCCATAC 
AGATCCTCGT CACACTTTGC CACTGAGTGG ACATATCCAC TGTCTCTGGC TAACGTGAGT 
GTACAGCCTG GCCGGAGACC TCAGGAGCTA CATGTGAGCT GGGTGGAGTC AGGTGGTGGC 
AGAGACCACT TGGTACAGCT CTCAGTGGCC GAGTCCCTAT CCATCATAAA GAATGTGTCT 
GTCCCCCGTG GAGTCAACCA GCTTGACCTG GAGGGGCTGG TTCCAGGGTC TCGATACCGT 
GTGGAGATCA TTTCTCAGGC TGGGCCTCAT CGCACCTCCT CTCAGACTGC CATTGGCTAC 
ACTGTCCCAC TACCTCCGCT TTCCCTCTCG GCAAGCCCTG TCAGCACTGC CTGGGCTCTG 
GCTGTGCACT GGGAGACCCC TTCTGGGCAG AGGGATGGAT ATCTGCTCAG TGTGCATGAG 
GAAGGTTCTT CTAAACCAGC AAGGAACCTG AAAGTAGGGA AAGACAGCAC CAATGTCACC 
CTGGCACAGC TGGAACCAGG AACTTGCTAC CTTGTTGAGA TCTGGGCTGT AGCTGGACCT 
TACCGCTCAC TCCCCAAGAA CTTCACTGGC TGCACAGTTC CTGCTGCACC GACAGACCTG 
AGCCTCACCA ACCCAGGCAG CTCCTCAGAG CTTTACACAT GCTGGAACAA GCCCCCCGGC 
AGGAGGGATC GCTACCATGT TATCCTGTAT GGCCTCAGCA TCCAGAGTAG GAATCGGGTC 
CAGACCTTGA GTCCAGATGC CCAGAACATC ACCTGGACTC ACCTGGAGGC AGGCAGCAGG 
TTTGCTGTGC AGGTCACTGC TGTGAAAGGT TCATTAGAAG CCTCCTCCAC CAACGTCACC 
CAATGGACAT ATCCCTTGGC CCCTGCCAAC CTCACCCTGG GCAGCCCTTC TGCCTCCGAG 
CTGCAGGCCT CCTGGGCAAC AACGGGGCGA GGAGCAGAAG GCTATGTGGT GGATGTCTAT 
GATAGAGCCT CCAGCAGTCA CGTTGGGCAC GTGGTGCTGG GTGGGGATGC CCGGAGTCAC 
ACCTTCAGAA AGCTGAGCCC TGGTACTCGC TACAGCGTGG CAGTGAGGGC CACAGCTGGG 
CCCTTCCACA CCAGCACCCC CAACTTCACC CTCTGCACAC GCCCGCTGCC CCCAGCCGCC 
GTGCGCTTGC TGAGCACGGG GCACCCCGAC AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT 
GGGGGGCGAG ACGGCTACAC ACTGACCCTG TACCACGGGC GGCTGGGCAG CATGGTGGCT 
ACCACCTCTC TTGGGAGAGA CACCCACAAC TTCACCTTCA TGGGTCTGAA CCCAGGATAC 
AAGTACTCCC TGGAGGCCAG TGCCATGGCT GGACCATACC ACGCAGCAGC ACCCAACATC 
AGTGCCTGGA CACGCCCGCT GCCCCCAGCC TCCGTGCGCT TGCTGAGCAC GGGGCACCCC 
GACAGGCTGA GTGCGTCCTG GGCAGCCGCG GCTGGGGGGC GAGACGGCTA CACACTGACC 
CTGTACCACG GGCGGCTGGG CAGCATGGTG GCTACCACCT CTCTTGGGAG AGACACCCAC 
AACTTCACCT TCACGGGCCT GAACCCAGGA AGCAAGTATG TTCTGGAGGC GGGATCCATA 
GCAGGGTCCT ACCAGACACC TGCAGGGAAC ATCAGCAACT GGACGTACCC CCTGGCACCT 
CGTAACGTGT ACATGACGAA CCAGGGGTAT CCCAACAGGC TGAGTGTGTC CTGGCGAGCT 
GAACCCCAAG GGCAAGACAG TTACAGGCTC CTCCTGTATC ATTCGGGATC AGGTATTCTG 
ACAGCCAATG TATCTGTTGA GAAAGGCACC AGCAAATTCA CCTTTTCTGG CCTGGCTCCG 
GGACATAAAT ACCTGCTGGA AGTGGTGTCC ACGGCAGGGC CCTACGCAGC CTCTGCAGGG 
AACATCAGTG ACTGGACAAC CCCCTCAGTT CCCAAAAATC TCTCTGCGAT GGCTGAAGGG 
AACAACACAG TGCTCATCTC CTGGGACAGT GTCTCTGGAC AACAGGATGA GTGCCAGTTG 
TGGCTGCGGG ATCCCAGGAA CAGCACACTG CCCTGGCGGC ACGCTCTCAG CCAAGGGCAA 
GTCCACCACC TCCTCCAGGG GCTGGTCCCA GGGAGGAACT ACTCTGTCTC CTTGAGCTGC 
GTGGCCGGGC CCTACTGGAG CAGCACCAAA CCCTTGGCGG TGCTGATGGA GCCAAACCCA 
GTGGAAGATG TGCAATGCTT ATCAGAGTCA AGGAGCCTTT ACTTGAACTG GACCAGCTCT 
CCTGGAGATG TGGAGGCTTA TGAAGTGGTG ACAGAGAGAC TTTCTGATGG ACTTCCCACC 
TCCAAGTATG TCATGAGCAT CCCTATGAGT GAGGCCAGCC TGGAGGGGCT GCAGCCAAAC 
TCGTCATACC GAATCGCTGT GAGCACAGTG GGCATGAACA CAATTAGGAG CCAGGCTGTG 
ACTCTGCTCT GCAACACAAC AGTGGAGGCC TTGCCTCCGC CCCTGCGAGC AGACATCTTC 
CAAGTAGAGG CCAGCTCCGC AGTTATCATT TCATCCGACC TGTTCAGTGA AGAGAATGGC 
CAGATTGAGT ATTACGGTGT CGTTGCTACC ACTAATGATT CACTGCTGAG GCCCACCCAG 
GAAATCATGT CCAGCACATG GTATGACCAC TATTACGGGA CAGAGGACTC CTACCTGGCT 
GTGCTAATCC CCAATCCTTT CCATTCGAGC CCCAGGAGCT CCCCCGAAAC CTGGCGAGTG 
CCAGTGGGAA TAGAGGAGTG CGGCCAGTCC AGGGCAATGT GCAACGGGAA GCTGAAAGCC 
AACGAGCAGT ACAGGTTCAG CATTGCTGCC TTCACCAAAT ACGACCCAGT AGCCCCTGCA 
GTGACCTTCA CCATGTTCTC AGCTGCTGAA TCCAGTGCAG ATGCAACTCC ACTCTCAATG 
CCAATAATCA CTGGAATCAT CGTGGGATTT CTCTTGACAC TTGCAGCTAT TTTTGCTTTG 
GTCTACTGGA AACAGCTCAG AGCAAAGAGA ACCAACAAAA GCAGCCTGCC CCAGGAAATG 
GTGACCTACA GCTTGAGAAA CATCCATCGG CCAATTCCCA TACAGAACTT CAAGCAGTAC 
TATGAGTTGA AGACAGCAAG TGCTAACCAC GCTTTTTTCC AGGAGTTTGA GGAGCTGAAG 
GAAGTTGGGA AGGAGCAGCC AAAGGTGGAG GCTGAGCTAC CAGCAAATGT CTCCAAGAAC 
AGATATCCCC ACGTGCTGCC CTATGATCAC TCTCGGGTCA AGCTGAGCCA ACTGGGGGAG 
GATCCACACT CTGACTACAT CAATGCCAAC TTCATGCCTG GCTATACATC CCAGCAGGAG 
TTCATTGCCA CCCAGGGGCC CCTGAAGAAA ACGATAGAGG ACTTCTGGAG GCTGGTGTGG 
GAGCAGAATG TCTGCAACAT CATCATGCTG ACAGTATGCA TGGAGAACGG GCGGGTTCTC 
TGTGATCACT ACTGGCCATC CGAATCTGCC CCAGTCTCCT ATGGGCACAT CCGTGTCCAC 
TTGCTGTCAC AGAGCAGCTC CGAGGAGTGG ACGATGCGCG AGTTCAAACT GTGGCATGAG 
GGTATCCGGG CAGAGCGGCA TGTGTCCCAC CTGCACTACA CGGCATGGCC TGACCATGGG 
ATCCCAGAGT CCACGACTTC CATTATGACC TTCAGAGAGC TGGTCCGGGA GCGTATCCAG 
AGCACCAAGG ACGCGGGGCC AACCCTCGTG CACTGCAGCG CTGGGGTGGG CCGCACTGGC 
ACCTTCATTG CACTGGACCG GCTGCTGCAG CAGATGAAGC AGGAGAAGGT GGTGGACACG 
TTTGGTGTTG TTTACACCTT GCGGATGAAC CGTTACCTGA TGATTCAGAC GCTGTCCCAG 
TATATTTTCC TGCACAGCTG TATTCTGGAA AAGATCTTAG AGGAACCACT CCTGGGTTTA 
TCAGGGACAG AGAGGTCCTG CCCCATCCCG CTGAAAAGCT TTGCTCAGCA CTATGCCCAG 
AAGGCAACCA AGTCGCATGT GGGCTTCCTG AGGGAGTACG AGACCCTCCT AGAGGCTGTC 
AAGGAAGAAG CCAGCTCTGC AACACCATCT TCAGGCGACC AGCAGACACA TCCCTCTTCC 
AGTATCCTCC CATATGATCG CTCCAGAGTG AAGTTTTCCC TGCTGGAACA GGGCCCATTC 
TCAGGTCTCC TGCAGGTGTG GCGTGTCCCG GGCTGCAGCT CCAGCAGGGA ATATCTGGCT 
GTCCAGGGGC CTGACAAGTT GACTGTGGAG GACTTCTGGA CCCTGGTGTG GGAACAGGAC 
GTTCACACCA TCCTAACACT TCTCCCATGG CAGGAGAAGG GGGAGATCCC AAGTGGGGCG 
TGCTGGCCCC TGGAAGGAGA CTCTCTCTGC ACAAAGACGC TGACCATCCA GTGTGGCACA 
GAGAAGCTTG TCTCGGGATG GAGGTGCATC CAGCTCAAAA TGAAACATGA GAAGAAAGCA 
AAGGAGAGGC AGGTACAACA GTTCCTATAC ACACTGTGGA GCAGCAAGAA ACAGCCAGAT 
GTCCAAAGCC TGGTGGAGTT CCTCACTGCT GTCAGACGGT GCATGCCCCA CCGAAAGAGA 
GCCGGTCCTC TCCTGCTGCA CTGCAGCAGT GGCGTGAGCC AAATGGGGAC ACTGATCTCC 
CTGGACTGCC TGTTGCACCA GATGAAAGCT GAGAGAACGG TGGATGTCTA TGGGGTCACC 
CTCCAGCTGG CAAGAAGCTG TTGTCTCATG ACCCCAACAC TGGACCAGTA CATGTTCCTG 
TACACCTGCA TCCGGGACAT CATTGCTCAG AAACAGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106890484 ( XM_014946344.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801830.1 Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X2, mRNA.

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CloneID	OCa68220
Clone ID Related Accession (Same CDS sequence)	XM_014946344.1
Accession Version	XM_014946344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	receptor-type tyrosine-protein phosphatase V-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACCGC CGCCGCCGCT GCTGCTGCTC GTCCTGTGGG TGCTGCGGCT CCTGGGAACC 
CTGGCAGAGG ATGAGGGATG CAACCAGACA GCACGGGCAG GGTTGGAGGG ACAGGATGCA 
CCCAATGATA GAGGGACCCT CCTGAACCTC AGTGTGAGTG ACCATGGCTG GTCGGACTCC 
CTCCTTCTGT CCTGGGATGA GCCAGAGGGG GGTGCCAAAG GATATTCGCT CGCTCTCTCC 
TCCCTGGGGT CAGGCACACC GTTGCAGAAT GGATCTGCTG GACCCAACAT CACCAGCTTC 
TGGTTCCATG GGCTGACCCC TGGCATGCGC TATGAGATTG AGGTGACAGC CACACTTGCC 
TGCATGGAGA CCACCAGCCA GACAGTCACA GCACAGACAA GTCCTTCACC TGTCCGCAAC 
CTGACCCTGA GCAGTGGTGG GAGCTCTGCC ATGCTGCAGG CGTCCTGGGC GCATGCCCAC 
GGGCAGCGAG ACAGCTACCA CCTAGCCCTC TACCACAGCG ACTCCCAGAC GCTGGTGAGA 
AATGTGTCCA TCCTGCCAAA CGCCTCTACC TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT 
GAGTACGCCT TGAAGGTCAG CACGCTGGTT GGATCCAGTC AGGCGAGCAC CAGTATCCAC 
CAGTGGACAG CTCCCTCCAT CCCCACCCAG CTGAGACTCA GCCCAGGATC CAGCACCAGC 
CTCATCGCCT CCTGGGCAGA TGCTGGGGGG GCTGCCTGGC TGCACCTCAC ACTCCACAAC 
CTCCTCACCC AGATGGTGAC CACCACCCTG TCAGCCAGAA GGGGCCTCAC CAGCTACACC 
TTCCAGCATC TCCACTCCGG CACCCAGTAC TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC 
TACACCATGG TGGGGCCCAA TGCCACCGCT TGGACATACC CCCTGAGCCC TGGAAATGTG 
ACCCTGAGCC CTGCAGAGGA ACAGAACTCC TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA 
GAGAGGGACT TCTACCTGGT GACACTGCAG GAGGAAGAGG ATGGTGTGCC AACAAGGAAC 
ATCTCTGTCG ATGGAGACAA CAGTCATGTC ACTTTCCACG GGCTGGATCC TGGGAAGCAA 
TACTCTGTCC AGGTGACAGC AGTGGCTGGG CCTTACAGAG CATCAGCCCA CAGCACAGCA 
GCCTGGACAC AGCCGCTAGC ACCATCTGGT GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC 
AGCCTACTGG CCAGCTGGGA GGAGGCAATG GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC 
ATGGAGCACC CTGTGAAGAA TAGCTCGCTG GTTAAAGGTG TCACCAACTT CACCTACAAC 
GGCCTCCACC CTGGGACTCT TTACACCTTT GAGGTCAGCA CCGTGGCTGG CCCCTACACA 
TCCTCGCCCC GGCGCATCAC CAACTGGACA TATCCTTTGC CCCTGGAGCA GCTGACCCTC 
AGCAATCAGG GCCACAGCAC CTCTCTGCAA GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC 
ACTGGCTACG CAGGCACCCT CTGGGAAACC AAGTCCCAGG AGCGGGTCAG GAACATGACT 
GTGGGGAACA ACTGGATGAA TGCCACCTTT GAGGACCTGG TCCCTGGGCG GCAGTATACA 
CTGGAGATGA CTGCTATGGC CGGACCTTAC AGATCCCCTG TGCGATCAGC CACAGACTGG 
ACATACCCTC TGGCTCCAGC CGGCGTGACC CTGACCAACA CCCGACGCCC ACTGGGACTT 
GCTGCCTTCT GGGACAAGGC TGCTGGTGAT GTGGACCAGT TCCACCTCCA GCTCTACAGC 
AAGAGCCATC CAGCACAGAG GAACATCTCA GTAGGGCCAG ACACCCACAA CTTCACATTC 
CTAGGGCTGT CCCCTGGGAT TCAGTACTTC CTGAAGGTGA CTGTCCTCGC TGGCCCATAC 
AGATCCTCGT CACACTTTGC CACTGAGTGG ACATATCCAC TGTCTCTGGC TAACGTGAGT 
GTACAGCCTG GCCGGAGACC TCAGGAGCTA CATGTGAGCT GGGTGGAGTC AGGTGGTGGC 
AGAGACCACT TGGTACAGCT CTCAGTGGCC GAGTCCCTAT CCATCATAAA GAATGTGTCT 
GTCCCCCGTG GAGTCAACCA GCTTGACCTG GAGGGGCTGG TTCCAGGGTC TCGATACCGT 
GTGGAGATCA TTTCTCAGGC TGGGCCTCAT CGCACCTCCT CTCAGACTGC CATTGGCTAC 
ACTGTCCCAC TACCTCCGCT TTCCCTCTCG GCAAGCCCTG TCAGCACTGC CTGGGCTCTG 
GCTGTGCACT GGGAGACCCC TTCTGGGCAG AGGGATGGAT ATCTGCTCAG TGTGCATGAG 
GAAGGTTCTT CTAAACCAGC AAGGAACCTG AAAGTAGGGA AAGACAGCAC CAATGTCACC 
CTGGCACAGC TGGAACCAGG AACTTGCTAC CTTGTTGAGA TCTGGGCTGT AGCTGGACCT 
TACCGCTCAC TCCCCAAGAA CTTCACTGGC TGCACAGTTC CTGCTGCACC GACAGACCTG 
AGCCTCACCA ACCCAGGCAG CTCCTCAGAG CTTTACACAT GCTGGAACAA GCCCCCCGGC 
AGGAGGGATC GCTACCATGT TATCCTGTAT GGCCTCAGCA TCCAGAGTAG GAATCGGGTC 
CAGACCTTGA GTCCAGATGC CCAGAACATC ACCTGGACTC ACCTGGAGGC AGGCAGCAGG 
TTTGCTGTGC AGGTCACTGC TGTGAAAGGT TCATTAGAAG CCTCCTCCAC CAACGTCACC 
CAATGGACAT ATCCCTTGGC CCCTGCCAAC CTCACCCTGG GCAGCCCTTC TGCCTCCGAG 
CTGCAGGCCT CCTGGGCAAC AACGGGGCGA GGAGCAGAAG GCTATGTGGT GGATGTCTAT 
GATAGAGCCT CCAGCAGTCA CGTTGGGCAC GTGGTGCTGG GTGGGGATGC CCGGAGTCAC 
ACCTTCAGAA AGCTGAGCCC TGGTACTCGC TACAGCGTGG CAGTGAGGGC CACAGCTGGG 
CCCTTCCACA CCAGCACCCC CAACTTCACC CTCTGCACAC GCCCGCTGCC CCCAGCCGCC 
GTGCGCTTGC TGAGCACGGG GCACCCCGAC AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT 
GGGGGGCGAG ACGGCTACAC ACTGACCCTG TACCACGGGC GGCTGGGCAG CATGGTGGCT 
ACCACCTCTC TTGGGAGAGA CACCCACAAC TTCACCTTCA TGGGTCTGAA CCCAGGATAC 
AAGTACTCCC TGGAGGCCAG TGCCATGGCT GGACCATACC ACGCAGCAGC ACCCAACATC 
AGTGCCTGGA CACGCCCGCT GCCCCCAGCC TCCGTGCGCT TGCTGAGCAC GGGGCACCCC 
GACAGGCTGA GTGCGTCCTG GGCAGCCGCG GCTGGGGGGC GAGACGGCTA CACACTGACC 
CTGTACCACG GGCGGCTGGG CAGCATGGTG GCTACCACCT CTCTTGGGAG AGACACCCAC 
AACTTCACCT TCACGGGCCT GAACCCAGGA AGCAAGTATG TTCTGGAGGC GGGATCCATA 
GCAGGGTCCT ACCAGACACC TGCAGGGAAC ATCAGCAACT GGACGTACCC CCTGGCACCT 
CGTAACGTGT ACATGACGAA CCAGGGGTAT CCCAACAGGC TGAGTGTGTC CTGGCGAGCT 
GAACCCCAAG GGCAAGACAG TTACAGGCTC CTCCTGTATC ATTCGGGATC AGGTATTCTG 
ACAGCCAATG TATCTGTTGA GAAAGGCACC AGCAAATTCA CCTTTTCTGG CCTGGCTCCG 
GGACATAAAT ACCTGCTGGA AGTGGTGTCC ACGGCAGGGC CCTACGCAGC CTCTGCAGGG 
AACATCAGTG ACTGGACAAC CCCCTCAGTT CCCAAAAATC TCTCTGCGAT GGCTGAAGGG 
AACAACACAG TGCTCATCTC CTGGGACAGT GTCTCTGGAC AACAGGATGA GTGCCAGTTG 
TGGCTGCGGG ATCCCAGGAA CAGCACACTG CCCTGGCGGC ACGCTCTCAG CCAAGGGCAA 
GTCCACCACC TCCTCCAGGG GCTGGTCCCA GGGAGGAACT ACTCTGTCTC CTTGAGCTGC 
GTGGCCGGGC CCTACTGGAG CAGCACCAAA CCCTTGGCGG TGCTGATGGA GCCAAACCCA 
GTGGAAGATG TGCAATGCTT ATCAGAGTCA AGGAGCCTTT ACTTGAACTG GACCAGCTCT 
CCTGGAGATG TGGAGGCTTA TGAAGTGGTG ACAGAGAGAC TTTCTGATGG ACTTCCCACC 
TCCAAGTATG TCATGAGCAT CCCTATGAGT GAGGCCAGCC TGGAGGGGCT GCAGCCAAAC 
TCGTCATACC GAATCGCTGT GAGCACAGTG GGCATGAACA CAATTAGGAG CCAGGCTGTG 
ACTCTGCTCT GCAACACAAC AGTGGAGGCC TTGCCTCCGC CCCTGCGAGC AGACATCTTC 
CAAGTAGAGG CCAGCTCCGC AGTTATCATT TCATCCGACC TGTTCAGTGA AGAGAATGGC 
CAGATTGAGT ATTACGGTGT CGTTGCTACC ACTAATGATT CACTGCTGAG GCCCACCCAG 
GAAATCATGT CCAGCACATG GTATGACCAC TATTACGGGA CAGAGGACTC CTACCTGGCT 
GTGCTAATCC CCAATCCTTT CCATTCGAGC CCCAGGAGCT CCCCCGAAAC CTGGCGAGTG 
CCAGTGGGAA TAGAGGAGTG CGGCCAGTCC AGGGCAATGT GCAACGGGAA GCTGAAAGCC 
AACGAGCAGT ACAGGTTCAG CATTGCTGCC TTCACCAAAT ACGACCCAGT AGCCCCTGCA 
GTGACCTTCA CCATGTTCTC AGCTGCTGAA TCCAGTGCAG ATGCAACTCC ACTCTCAATG 
CCAATAATCA CTGGAATCAT CGTGGGATTT CTCTTGACAC TTGCAGCTAT TTTTGCTTTG 
GTCTACTGGA AACAGCTCAG AGCAAAGAGA ACCAACAAAA GCAGCCTGCC CCAGGAAATG 
GTGACCTACA GCTTGAGAAA CATCCATCGG CCAATTCCCA TACAGAACTT CAAGCAGTAC 
TATGAGTTGA AGACAGCAAG TGCTAACCAC GCTTTTTTCC AGGAGTTTGA GGAGCTGAAG 
GAAGTTGGGA AGGAGCAGCC AAAGGTGGAG GCTGAGCTAC CAGCAAATGT CTCCAAGAAC 
AGATATCCCC ACGTGCTGCC CTATGATCAC TCTCGGGTCA AGCTGAGCCA ACTGGGGGAG 
GATCCACACT CTGACTACAT CAATGCCAAC TTCATGCCTG GCTATACATC CCAGCAGGAG 
TTCATTGCCA CCCAGGGGCC CCTGAAGAAA ACGATAGAGG ACTTCTGGAG GCTGGTGTGG 
GAGCAGAATG TCTGCAACAT CATCATGCTG ACAGTATGCA TGGAGAACGG GCGGGTTCTC 
TGTGATCACT ACTGGCCATC CGAATCTGCC CCAGTCTCCT ATGGGCACAT CCGTGTCCAC 
TTGCTGTCAC AGAGCAGCTC CGAGGAGTGG ACGATGCGCG AGTTCAAACT GTGGCATGAG 
GGTATCCGGG CAGAGCGGCA TGTGTCCCAC CTGCACTACA CGGCATGGCC TGACCATGGG 
ATCCCAGAGT CCACGACTTC CATTATGACC TTCAGAGAGC TGGTCCGGGA GCGTATCCAG 
AGCACCAAGG ACGCGGGGCC AACCCTCGTG CACTGCAGCG CTGGGGTGGG CCGCACTGGC 
ACCTTCATTG CACTGGACCG GCTGCTGCAG CAGATGAAGC AGGAGAAGGT GGTGGACACG 
TTTGGTGTTG TTTACACCTT GCGGATGAAC CGTTACCTGA TGATTCAGAC GCTGTCCCAG 
TATATTTTCC TGCACAGCTG TATTCTGGAA AAGATCTTAG AGGAACCACT CCTGGGTTTA 
TCAGGGACAG AGAGGTCCTG CCCCATCCCG CTGAAAAGCT TTGCTCAGCA CTATGCCCAG 
AAGGCAACCA AGTCGCATGT GGGCTTCCTG AGGGAGTACG AGACCCTCCT AGAGGCTGTC 
AAGGAAGAAG CCAGCTCTGC AACACCATCT TCAGGCGACC AGCAGACACA TCCCTCTTCC 
AGTATCCTCC CATATGATCG CTCCAGAGTG AAGTTTTCCC TGCTGGAACA GGGCCCATTC 
TCAGGTCTCC TGCAGGTGTG GCGTGTCCCG GGCTGCAGCT CCAGCAGGGA ATATCTGGCT 
GTCCAGGGGC CTGACAAGTT GACTGTGGAG GACTTCTGGA CCCTGGTGTG GGAACAGGAC 
GTTCACACCA TCCTAACACT TCTCCCATGG CAGGAGAAGG GGGAGATCCC AAGTGGGGCG 
TGCTGGCCCC TGGAAGGAGA CTCTCTCTGC ACAAAGACGC TGACCATCCA GTGTGGCACA 
GAGAAGCTTG TCTCGGGATG GAGGTGCATC CAGCTCAAAA TGAAACATGA GAAGAAAGCA 
AAGGAGAGGC AGGTACAACA GTTCCTATAC ACACTGTGGA GCAGCAAGAA ACAGCCAGAT 
GTCCAAAGCC TGGTGGAGTT CCTCACTGCT GTCAGACGGT GCATGCCCCA CCGAAAGAGA 
GCCGGTCCTC TCCTGCTGCA CTGCAGTGGC GTGAGCCAAA TGGGGACACT GATCTCCCTG 
GACTGCCTGT TGCACCAGAT GAAAGCTGAG AGAACGGTGG ATGTCTATGG GGTCACCCTC 
CAGCTGGCAA GAAGCTGTTG TCTCATGACC CCAACACTGG ACCAGTACAT GTTCCTGTAC 
ACCTGCATCC GGGACATCAT TGCTCAGAAA CAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801830.1
CDS	422..7240
Translation	MRPPPPLLLLVLWVLRLLGTLAEDEGCNQTARAGLEGQDAPNDRGTLLNLSVSDHGWSDSLLLSWDEPEGGAKGYSLALSSLGSGTPLQNGSAGPNITSFWFHGLTPGMRYEIEVTATLACMETTSQTVTAQTSPSPVRNLTLSSGGSSAMLQASWAHAHGQRDSYHLALYHSDSQTLVRNVSILPNASTFLFDGLLPGSEYALKVSTLVGSSQASTSIHQWTAPSIPTQLRLSPGSSTSLIASWADAGGAAWLHLTLHNLLTQMVTTTLSARRGLTSYTFQHLHSGTQYWLGLSAMAGPYTMVGPNATAWTYPLSPGNVTLSPAEEQNSLQARWSTPAGERDFYLVTLQEEEDGVPTRNISVDGDNSHVTFHGLDPGKQYSVQVTAVAGPYRASAHSTAAWTQPLAPSGVSLSSQGSPYSLLASWEEAMGEGYVLALSPMEHPVKNSSLVKGVTNFTYNGLHPGTLYTFEVSTVAGPYTSSPRRITNWTYPLPLEQLTLSNQGHSTSLQASWRAAPAGSTGYAGTLWETKSQERVRNMTVGNNWMNATFEDLVPGRQYTLEMTAMAGPYRSPVRSATDWTYPLAPAGVTLTNTRRPLGLAAFWDKAAGDVDQFHLQLYSKSHPAQRNISVGPDTHNFTFLGLSPGIQYFLKVTVLAGPYRSSSHFATEWTYPLSLANVSVQPGRRPQELHVSWVESGGGRDHLVQLSVAESLSIIKNVSVPRGVNQLDLEGLVPGSRYRVEIISQAGPHRTSSQTAIGYTVPLPPLSLSASPVSTAWALAVHWETPSGQRDGYLLSVHEEGSSKPARNLKVGKDSTNVTLAQLEPGTCYLVEIWAVAGPYRSLPKNFTGCTVPAAPTDLSLTNPGSSSELYTCWNKPPGRRDRYHVILYGLSIQSRNRVQTLSPDAQNITWTHLEAGSRFAVQVTAVKGSLEASSTNVTQWTYPLAPANLTLGSPSASELQASWATTGRGAEGYVVDVYDRASSSHVGHVVLGGDARSHTFRKLSPGTRYSVAVRATAGPFHTSTPNFTLCTRPLPPAAVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFMGLNPGYKYSLEASAMAGPYHAAAPNISAWTRPLPPASVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFTGLNPGSKYVLEAGSIAGSYQTPAGNISNWTYPLAPRNVYMTNQGYPNRLSVSWRAEPQGQDSYRLLLYHSGSGILTANVSVEKGTSKFTFSGLAPGHKYLLEVVSTAGPYAASAGNISDWTTPSVPKNLSAMAEGNNTVLISWDSVSGQQDECQLWLRDPRNSTLPWRHALSQGQVHHLLQGLVPGRNYSVSLSCVAGPYWSSTKPLAVLMEPNPVEDVQCLSESRSLYLNWTSSPGDVEAYEVVTERLSDGLPTSKYVMSIPMSEASLEGLQPNSSYRIAVSTVGMNTIRSQAVTLLCNTTVEALPPPLRADIFQVEASSAVIISSDLFSEENGQIEYYGVVATTNDSLLRPTQEIMSSTWYDHYYGTEDSYLAVLIPNPFHSSPRSSPETWRVPVGIEECGQSRAMCNGKLKANEQYRFSIAAFTKYDPVAPAVTFTMFSAAESSADATPLSMPIITGIIVGFLLTLAAIFALVYWKQLRAKRTNKSSLPQEMVTYSLRNIHRPIPIQNFKQYYELKTASANHAFFQEFEELKEVGKEQPKVEAELPANVSKNRYPHVLPYDHSRVKLSQLGEDPHSDYINANFMPGYTSQQEFIATQGPLKKTIEDFWRLVWEQNVCNIIMLTVCMENGRVLCDHYWPSESAPVSYGHIRVHLLSQSSSEEWTMREFKLWHEGIRAERHVSHLHYTAWPDHGIPESTTSIMTFRELVRERIQSTKDAGPTLVHCSAGVGRTGTFIALDRLLQQMKQEKVVDTFGVVYTLRMNRYLMIQTLSQYIFLHSCILEKILEEPLLGLSGTERSCPIPLKSFAQHYAQKATKSHVGFLREYETLLEAVKEEASSATPSSGDQQTHPSSSILPYDRSRVKFSLLEQGPFSGLLQVWRVPGCSSSREYLAVQGPDKLTVEDFWTLVWEQDVHTILTLLPWQEKGEIPSGACWPLEGDSLCTKTLTIQCGTEKLVSGWRCIQLKMKHEKKAKERQVQQFLYTLWSSKKQPDVQSLVEFLTAVRRCMPHRKRAGPLLLHCSGVSQMGTLISLDCLLHQMKAERTVDVYGVTLQLARSCCLMTPTLDQYMFLYTCIRDIIAQKQP

Target ORF information:

RefSeq Version	XM_014946344.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946344.1

ORF Insert Sequence:

ATGCGACCGC CGCCGCCGCT GCTGCTGCTC GTCCTGTGGG TGCTGCGGCT CCTGGGAACC 
CTGGCAGAGG ATGAGGGATG CAACCAGACA GCACGGGCAG GGTTGGAGGG ACAGGATGCA 
CCCAATGATA GAGGGACCCT CCTGAACCTC AGTGTGAGTG ACCATGGCTG GTCGGACTCC 
CTCCTTCTGT CCTGGGATGA GCCAGAGGGG GGTGCCAAAG GATATTCGCT CGCTCTCTCC 
TCCCTGGGGT CAGGCACACC GTTGCAGAAT GGATCTGCTG GACCCAACAT CACCAGCTTC 
TGGTTCCATG GGCTGACCCC TGGCATGCGC TATGAGATTG AGGTGACAGC CACACTTGCC 
TGCATGGAGA CCACCAGCCA GACAGTCACA GCACAGACAA GTCCTTCACC TGTCCGCAAC 
CTGACCCTGA GCAGTGGTGG GAGCTCTGCC ATGCTGCAGG CGTCCTGGGC GCATGCCCAC 
GGGCAGCGAG ACAGCTACCA CCTAGCCCTC TACCACAGCG ACTCCCAGAC GCTGGTGAGA 
AATGTGTCCA TCCTGCCAAA CGCCTCTACC TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT 
GAGTACGCCT TGAAGGTCAG CACGCTGGTT GGATCCAGTC AGGCGAGCAC CAGTATCCAC 
CAGTGGACAG CTCCCTCCAT CCCCACCCAG CTGAGACTCA GCCCAGGATC CAGCACCAGC 
CTCATCGCCT CCTGGGCAGA TGCTGGGGGG GCTGCCTGGC TGCACCTCAC ACTCCACAAC 
CTCCTCACCC AGATGGTGAC CACCACCCTG TCAGCCAGAA GGGGCCTCAC CAGCTACACC 
TTCCAGCATC TCCACTCCGG CACCCAGTAC TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC 
TACACCATGG TGGGGCCCAA TGCCACCGCT TGGACATACC CCCTGAGCCC TGGAAATGTG 
ACCCTGAGCC CTGCAGAGGA ACAGAACTCC TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA 
GAGAGGGACT TCTACCTGGT GACACTGCAG GAGGAAGAGG ATGGTGTGCC AACAAGGAAC 
ATCTCTGTCG ATGGAGACAA CAGTCATGTC ACTTTCCACG GGCTGGATCC TGGGAAGCAA 
TACTCTGTCC AGGTGACAGC AGTGGCTGGG CCTTACAGAG CATCAGCCCA CAGCACAGCA 
GCCTGGACAC AGCCGCTAGC ACCATCTGGT GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC 
AGCCTACTGG CCAGCTGGGA GGAGGCAATG GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC 
ATGGAGCACC CTGTGAAGAA TAGCTCGCTG GTTAAAGGTG TCACCAACTT CACCTACAAC 
GGCCTCCACC CTGGGACTCT TTACACCTTT GAGGTCAGCA CCGTGGCTGG CCCCTACACA 
TCCTCGCCCC GGCGCATCAC CAACTGGACA TATCCTTTGC CCCTGGAGCA GCTGACCCTC 
AGCAATCAGG GCCACAGCAC CTCTCTGCAA GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC 
ACTGGCTACG CAGGCACCCT CTGGGAAACC AAGTCCCAGG AGCGGGTCAG GAACATGACT 
GTGGGGAACA ACTGGATGAA TGCCACCTTT GAGGACCTGG TCCCTGGGCG GCAGTATACA 
CTGGAGATGA CTGCTATGGC CGGACCTTAC AGATCCCCTG TGCGATCAGC CACAGACTGG 
ACATACCCTC TGGCTCCAGC CGGCGTGACC CTGACCAACA CCCGACGCCC ACTGGGACTT 
GCTGCCTTCT GGGACAAGGC TGCTGGTGAT GTGGACCAGT TCCACCTCCA GCTCTACAGC 
AAGAGCCATC CAGCACAGAG GAACATCTCA GTAGGGCCAG ACACCCACAA CTTCACATTC 
CTAGGGCTGT CCCCTGGGAT TCAGTACTTC CTGAAGGTGA CTGTCCTCGC TGGCCCATAC 
AGATCCTCGT CACACTTTGC CACTGAGTGG ACATATCCAC TGTCTCTGGC TAACGTGAGT 
GTACAGCCTG GCCGGAGACC TCAGGAGCTA CATGTGAGCT GGGTGGAGTC AGGTGGTGGC 
AGAGACCACT TGGTACAGCT CTCAGTGGCC GAGTCCCTAT CCATCATAAA GAATGTGTCT 
GTCCCCCGTG GAGTCAACCA GCTTGACCTG GAGGGGCTGG TTCCAGGGTC TCGATACCGT 
GTGGAGATCA TTTCTCAGGC TGGGCCTCAT CGCACCTCCT CTCAGACTGC CATTGGCTAC 
ACTGTCCCAC TACCTCCGCT TTCCCTCTCG GCAAGCCCTG TCAGCACTGC CTGGGCTCTG 
GCTGTGCACT GGGAGACCCC TTCTGGGCAG AGGGATGGAT ATCTGCTCAG TGTGCATGAG 
GAAGGTTCTT CTAAACCAGC AAGGAACCTG AAAGTAGGGA AAGACAGCAC CAATGTCACC 
CTGGCACAGC TGGAACCAGG AACTTGCTAC CTTGTTGAGA TCTGGGCTGT AGCTGGACCT 
TACCGCTCAC TCCCCAAGAA CTTCACTGGC TGCACAGTTC CTGCTGCACC GACAGACCTG 
AGCCTCACCA ACCCAGGCAG CTCCTCAGAG CTTTACACAT GCTGGAACAA GCCCCCCGGC 
AGGAGGGATC GCTACCATGT TATCCTGTAT GGCCTCAGCA TCCAGAGTAG GAATCGGGTC 
CAGACCTTGA GTCCAGATGC CCAGAACATC ACCTGGACTC ACCTGGAGGC AGGCAGCAGG 
TTTGCTGTGC AGGTCACTGC TGTGAAAGGT TCATTAGAAG CCTCCTCCAC CAACGTCACC 
CAATGGACAT ATCCCTTGGC CCCTGCCAAC CTCACCCTGG GCAGCCCTTC TGCCTCCGAG 
CTGCAGGCCT CCTGGGCAAC AACGGGGCGA GGAGCAGAAG GCTATGTGGT GGATGTCTAT 
GATAGAGCCT CCAGCAGTCA CGTTGGGCAC GTGGTGCTGG GTGGGGATGC CCGGAGTCAC 
ACCTTCAGAA AGCTGAGCCC TGGTACTCGC TACAGCGTGG CAGTGAGGGC CACAGCTGGG 
CCCTTCCACA CCAGCACCCC CAACTTCACC CTCTGCACAC GCCCGCTGCC CCCAGCCGCC 
GTGCGCTTGC TGAGCACGGG GCACCCCGAC AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT 
GGGGGGCGAG ACGGCTACAC ACTGACCCTG TACCACGGGC GGCTGGGCAG CATGGTGGCT 
ACCACCTCTC TTGGGAGAGA CACCCACAAC TTCACCTTCA TGGGTCTGAA CCCAGGATAC 
AAGTACTCCC TGGAGGCCAG TGCCATGGCT GGACCATACC ACGCAGCAGC ACCCAACATC 
AGTGCCTGGA CACGCCCGCT GCCCCCAGCC TCCGTGCGCT TGCTGAGCAC GGGGCACCCC 
GACAGGCTGA GTGCGTCCTG GGCAGCCGCG GCTGGGGGGC GAGACGGCTA CACACTGACC 
CTGTACCACG GGCGGCTGGG CAGCATGGTG GCTACCACCT CTCTTGGGAG AGACACCCAC 
AACTTCACCT TCACGGGCCT GAACCCAGGA AGCAAGTATG TTCTGGAGGC GGGATCCATA 
GCAGGGTCCT ACCAGACACC TGCAGGGAAC ATCAGCAACT GGACGTACCC CCTGGCACCT 
CGTAACGTGT ACATGACGAA CCAGGGGTAT CCCAACAGGC TGAGTGTGTC CTGGCGAGCT 
GAACCCCAAG GGCAAGACAG TTACAGGCTC CTCCTGTATC ATTCGGGATC AGGTATTCTG 
ACAGCCAATG TATCTGTTGA GAAAGGCACC AGCAAATTCA CCTTTTCTGG CCTGGCTCCG 
GGACATAAAT ACCTGCTGGA AGTGGTGTCC ACGGCAGGGC CCTACGCAGC CTCTGCAGGG 
AACATCAGTG ACTGGACAAC CCCCTCAGTT CCCAAAAATC TCTCTGCGAT GGCTGAAGGG 
AACAACACAG TGCTCATCTC CTGGGACAGT GTCTCTGGAC AACAGGATGA GTGCCAGTTG 
TGGCTGCGGG ATCCCAGGAA CAGCACACTG CCCTGGCGGC ACGCTCTCAG CCAAGGGCAA 
GTCCACCACC TCCTCCAGGG GCTGGTCCCA GGGAGGAACT ACTCTGTCTC CTTGAGCTGC 
GTGGCCGGGC CCTACTGGAG CAGCACCAAA CCCTTGGCGG TGCTGATGGA GCCAAACCCA 
GTGGAAGATG TGCAATGCTT ATCAGAGTCA AGGAGCCTTT ACTTGAACTG GACCAGCTCT 
CCTGGAGATG TGGAGGCTTA TGAAGTGGTG ACAGAGAGAC TTTCTGATGG ACTTCCCACC 
TCCAAGTATG TCATGAGCAT CCCTATGAGT GAGGCCAGCC TGGAGGGGCT GCAGCCAAAC 
TCGTCATACC GAATCGCTGT GAGCACAGTG GGCATGAACA CAATTAGGAG CCAGGCTGTG 
ACTCTGCTCT GCAACACAAC AGTGGAGGCC TTGCCTCCGC CCCTGCGAGC AGACATCTTC 
CAAGTAGAGG CCAGCTCCGC AGTTATCATT TCATCCGACC TGTTCAGTGA AGAGAATGGC 
CAGATTGAGT ATTACGGTGT CGTTGCTACC ACTAATGATT CACTGCTGAG GCCCACCCAG 
GAAATCATGT CCAGCACATG GTATGACCAC TATTACGGGA CAGAGGACTC CTACCTGGCT 
GTGCTAATCC CCAATCCTTT CCATTCGAGC CCCAGGAGCT CCCCCGAAAC CTGGCGAGTG 
CCAGTGGGAA TAGAGGAGTG CGGCCAGTCC AGGGCAATGT GCAACGGGAA GCTGAAAGCC 
AACGAGCAGT ACAGGTTCAG CATTGCTGCC TTCACCAAAT ACGACCCAGT AGCCCCTGCA 
GTGACCTTCA CCATGTTCTC AGCTGCTGAA TCCAGTGCAG ATGCAACTCC ACTCTCAATG 
CCAATAATCA CTGGAATCAT CGTGGGATTT CTCTTGACAC TTGCAGCTAT TTTTGCTTTG 
GTCTACTGGA AACAGCTCAG AGCAAAGAGA ACCAACAAAA GCAGCCTGCC CCAGGAAATG 
GTGACCTACA GCTTGAGAAA CATCCATCGG CCAATTCCCA TACAGAACTT CAAGCAGTAC 
TATGAGTTGA AGACAGCAAG TGCTAACCAC GCTTTTTTCC AGGAGTTTGA GGAGCTGAAG 
GAAGTTGGGA AGGAGCAGCC AAAGGTGGAG GCTGAGCTAC CAGCAAATGT CTCCAAGAAC 
AGATATCCCC ACGTGCTGCC CTATGATCAC TCTCGGGTCA AGCTGAGCCA ACTGGGGGAG 
GATCCACACT CTGACTACAT CAATGCCAAC TTCATGCCTG GCTATACATC CCAGCAGGAG 
TTCATTGCCA CCCAGGGGCC CCTGAAGAAA ACGATAGAGG ACTTCTGGAG GCTGGTGTGG 
GAGCAGAATG TCTGCAACAT CATCATGCTG ACAGTATGCA TGGAGAACGG GCGGGTTCTC 
TGTGATCACT ACTGGCCATC CGAATCTGCC CCAGTCTCCT ATGGGCACAT CCGTGTCCAC 
TTGCTGTCAC AGAGCAGCTC CGAGGAGTGG ACGATGCGCG AGTTCAAACT GTGGCATGAG 
GGTATCCGGG CAGAGCGGCA TGTGTCCCAC CTGCACTACA CGGCATGGCC TGACCATGGG 
ATCCCAGAGT CCACGACTTC CATTATGACC TTCAGAGAGC TGGTCCGGGA GCGTATCCAG 
AGCACCAAGG ACGCGGGGCC AACCCTCGTG CACTGCAGCG CTGGGGTGGG CCGCACTGGC 
ACCTTCATTG CACTGGACCG GCTGCTGCAG CAGATGAAGC AGGAGAAGGT GGTGGACACG 
TTTGGTGTTG TTTACACCTT GCGGATGAAC CGTTACCTGA TGATTCAGAC GCTGTCCCAG 
TATATTTTCC TGCACAGCTG TATTCTGGAA AAGATCTTAG AGGAACCACT CCTGGGTTTA 
TCAGGGACAG AGAGGTCCTG CCCCATCCCG CTGAAAAGCT TTGCTCAGCA CTATGCCCAG 
AAGGCAACCA AGTCGCATGT GGGCTTCCTG AGGGAGTACG AGACCCTCCT AGAGGCTGTC 
AAGGAAGAAG CCAGCTCTGC AACACCATCT TCAGGCGACC AGCAGACACA TCCCTCTTCC 
AGTATCCTCC CATATGATCG CTCCAGAGTG AAGTTTTCCC TGCTGGAACA GGGCCCATTC 
TCAGGTCTCC TGCAGGTGTG GCGTGTCCCG GGCTGCAGCT CCAGCAGGGA ATATCTGGCT 
GTCCAGGGGC CTGACAAGTT GACTGTGGAG GACTTCTGGA CCCTGGTGTG GGAACAGGAC 
GTTCACACCA TCCTAACACT TCTCCCATGG CAGGAGAAGG GGGAGATCCC AAGTGGGGCG 
TGCTGGCCCC TGGAAGGAGA CTCTCTCTGC ACAAAGACGC TGACCATCCA GTGTGGCACA 
GAGAAGCTTG TCTCGGGATG GAGGTGCATC CAGCTCAAAA TGAAACATGA GAAGAAAGCA 
AAGGAGAGGC AGGTACAACA GTTCCTATAC ACACTGTGGA GCAGCAAGAA ACAGCCAGAT 
GTCCAAAGCC TGGTGGAGTT CCTCACTGCT GTCAGACGGT GCATGCCCCA CCGAAAGAGA 
GCCGGTCCTC TCCTGCTGCA CTGCAGTGGC GTGAGCCAAA TGGGGACACT GATCTCCCTG 
GACTGCCTGT TGCACCAGAT GAAAGCTGAG AGAACGGTGG ATGTCTATGG GGTCACCCTC 
CAGCTGGCAA GAAGCTGTTG TCTCATGACC CCAACACTGG ACCAGTACAT GTTCCTGTAC 
ACCTGCATCC GGGACATCAT TGCTCAGAAA CAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106890484 ( XM_014946345.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801831.1 Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X3, mRNA.

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CloneID	OCa68221
Clone ID Related Accession (Same CDS sequence)	XM_014946345.1
Accession Version	XM_014946345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	receptor-type tyrosine-protein phosphatase V-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACCGC CGCCGCCGCT GCTGCTGCTC GTCCTGTGGG TGCTGCGGCT CCTGGGAACC 
CTGGCAGAGG ATGAGGGATG CAACCAGACA GCACGGGCAG GGTTGGAGGG ACAGGATGCA 
CCCAATGATA GAGGGACCCT CCTGAACCTC AGTGTGAGTG ACCATGGCTG GTCGGACTCC 
CTCCTTCTGT CCTGGGATGA GCCAGAGGGG GGTGCCAAAG GATATTCGCT CGCTCTCTCC 
TCCCTGGGGT CAGGCACACC GTTGCAGAAT GGATCTGCTG GACCCAACAT CACCAGCTTC 
TGGTTCCATG GGCTGACCCC TGGCATGCGC TATGAGATTG AGGTGACAGC CACACTTGCC 
TGCATGGAGA CCACCAGCCA GACAGTCACA GCACAGACAA GTCCTTCACC TGTCCGCAAC 
CTGACCCTGA GCAGTGGTGG GAGCTCTGCC ATGCTGCAGG CGTCCTGGGC GCATGCCCAC 
GGGCAGCGAG ACAGCTACCA CCTAGCCCTC TACCACAGCG ACTCCCAGAC GCTGGTGAGA 
AATGTGTCCA TCCTGCCAAA CGCCTCTACC TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT 
GAGTACGCCT TGAAGGTCAG CACGCTGGTT GGATCCAGTC AGGCGAGCAC CAGTATCCAC 
CAGTGGACAG CTCCCTCCAT CCCCACCCAG CTGAGACTCA GCCCAGGATC CAGCACCAGC 
CTCATCGCCT CCTGGGCAGA TGCTGGGGGG GCTGCCTGGC TGCACCTCAC ACTCCACAAC 
CTCCTCACCC AGATGGTGAC CACCACCCTG TCAGCCAGAA GGGGCCTCAC CAGCTACACC 
TTCCAGCATC TCCACTCCGG CACCCAGTAC TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC 
TACACCATGG TGGGGCCCAA TGCCACCGCT TGGACATACC CCCTGAGCCC TGGAAATGTG 
ACCCTGAGCC CTGCAGAGGA ACAGAACTCC TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA 
GAGAGGGACT TCTACCTGGT GACACTGCAG GAGGAAGAGG ATGGTGTGCC AACAAGGAAC 
ATCTCTGTCG ATGGAGACAA CAGTCATGTC ACTTTCCACG GGCTGGATCC TGGGAAGCAA 
TACTCTGTCC AGGTGACAGC AGTGGCTGGG CCTTACAGAG CATCAGCCCA CAGCACAGCA 
GCCTGGACAC AGCCGCTAGC ACCATCTGGT GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC 
AGCCTACTGG CCAGCTGGGA GGAGGCAATG GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC 
ATGGAGCACC CTGTGAAGAA TAGCTCGCTG GTTAAAGGTG TCACCAACTT CACCTACAAC 
GGCCTCCACC CTGGGACTCT TTACACCTTT GAGGTCAGCA CCGTGGCTGG CCCCTACACA 
TCCTCGCCCC GGCGCATCAC CAACTGGACA TATCCTTTGC CCCTGGAGCA GCTGACCCTC 
AGCAATCAGG GCCACAGCAC CTCTCTGCAA GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC 
ACTGGCTACG CAGGCACCCT CTGGGAAACC AAGTCCCAGG AGCGGGTCAG GAACATGACT 
GTGGGGAACA ACTGGATGAA TGCCACCTTT GAGGACCTGG TCCCTGGGCG GCAGTATACA 
CTGGAGATGA CTGCTATGGC CGGACCTTAC AGATCCCCTG TGCGATCAGC CACAGACTGG 
ACATACCCTC TGGCTCCAGC CGGCGTGACC CTGACCAACA CCCGACGCCC ACTGGGACTT 
GCTGCCTTCT GGGACAAGGC TGCTGGTGAT GTGGACCAGT TCCACCTCCA GCTCTACAGC 
AAGAGCCATC CAGCACAGAG GAACATCTCA GTAGGGCCAG ACACCCACAA CTTCACATTC 
CTAGGGCTGT CCCCTGGGAT TCAGTACTTC CTGAAGGTGA CTGTCCTCGC TGGCCCATAC 
AGATCCTCGT CACACTTTGC CACTGAGTGG ACATATCCAC TGTCTCTGGC TAACGTGAGT 
GTACAGCCTG GCCGGAGACC TCAGGAGCTA CATGTGAGCT GGGTGGAGTC AGGTGGTGGC 
AGAGACCACT TGGTACAGCT CTCAGTGGCC GAGTCCCTAT CCATCATAAA GAATGTGTCT 
GTCCCCCGTG GAGTCAACCA GCTTGACCTG GAGGGGCTGG TTCCAGGGTC TCGATACCGT 
GTGGAGATCA TTTCTCAGGC TGGGCCTCAT CGCACCTCCT CTCAGACTGC CATTGGCTAC 
ACTGTCCCAC TACCTCCGCT TTCCCTCTCG GCAAGCCCTG TCAGCACTGC CTGGGCTCTG 
GCTGTGCACT GGGAGACCCC TTCTGGGCAG AGGGATGGAT ATCTGCTCAG TGTGCATGAG 
GAAGGTTCTT CTAAACCAGC AAGGAACCTG AAAGTAGGGA AAGACAGCAC CAATGTCACC 
CTGGCACAGC TGGAACCAGG AACTTGCTAC CTTGTTGAGA TCTGGGCTGT AGCTGGACCT 
TACCGCTCAC TCCCCAAGAA CTTCACTGGC TGCACAGTTC CTGCTGCACC GACAGACCTG 
AGCCTCACCA ACCCAGGCAG CTCCTCAGAG CTTTACACAT GCTGGAACAA GCCCCCCGGC 
AGGAGGGATC GCTACCATGT TATCCTGTAT GGCCTCAGCA TCCAGAGTAG GAATCGGGTC 
CAGACCTTGA GTCCAGATGC CCAGAACATC ACCTGGACTC ACCTGGAGGC AGGCAGCAGG 
TTTGCTGTGC AGGTCACTGC TGTGAAAGGT TCATTAGAAG CCTCCTCCAC CAACGTCACC 
CAATGGACAT ATCCCTTGGC CCCTGCCAAC CTCACCCTGG GCAGCCCTTC TGCCTCCGAG 
CTGCAGGCCT CCTGGGCAAC AACGGGGCGA GGAGCAGAAG GCTATGTGGT GGATGTCTAT 
GATAGAGCCT CCAGCAGTCA CGTTGGGCAC GTGGTGCTGG GTGGGGATGC CCGGAGTCAC 
ACCTTCAGAA AGCTGAGCCC TGGTACTCGC TACAGCGTGG CAGTGAGGGC CACAGCTGGG 
CCCTTCCACA CCAGCACCCC CAACTTCACC CTCTGCACAC GCCCGCTGCC CCCAGCCGCC 
GTGCGCTTGC TGAGCACGGG GCACCCCGAC AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT 
GGGGGGCGAG ACGGCTACAC ACTGACCCTG TACCACGGGC GGCTGGGCAG CATGGTGGCT 
ACCACCTCTC TTGGGAGAGA CACCCACAAC TTCACCTTCA TGGGTCTGAA CCCAGGATAC 
AAGTACTCCC TGGAGGCCAG TGCCATGGCT GGACCATACC ACGCAGCAGC ACCCAACATC 
AGTGCCTGGA CACGCCCGCT GCCCCCAGCC TCCGTGCGCT TGCTGAGCAC GGGGCACCCC 
GACAGGCTGA GTGCGTCCTG GGCAGCCGCG GCTGGGGGGC GAGACGGCTA CACACTGACC 
CTGTACCACG GGCGGCTGGG CAGCATGGTG GCTACCACCT CTCTTGGGAG AGACACCCAC 
AACTTCACCT TCACGGGCCT GAACCCAGGA AGCAAGTATG TTCTGGAGGC GGGATCCATA 
GCAGGGTCCT ACCAGACACC TGCAGGGAAC ATCAGCAACT GGACGTGTAT TCTGACAGCC 
AATGTATCTG TTGAGAAAGG CACCAGCAAA TTCACCTTTT CTGGCCTGGC TCCGGGACAT 
AAATACCTGC TGGAAGTGGT GTCCACGGCA GGGCCCTACG CAGCCTCTGC AGGGAACATC 
AGTGACTGGA CAACCCCCTC AGTTCCCAAA AATCTCTCTG CGATGGCTGA AGGGAACAAC 
ACAGTGCTCA TCTCCTGGGA CAGTGTCTCT GGACAACAGG ATGAGTGCCA GTTGTGGCTG 
CGGGATCCCA GGAACAGCAC ACTGCCCTGG CGGCACGCTC TCAGCCAAGG GCAAGTCCAC 
CACCTCCTCC AGGGGCTGGT CCCAGGGAGG AACTACTCTG TCTCCTTGAG CTGCGTGGCC 
GGGCCCTACT GGAGCAGCAC CAAACCCTTG GCGGTGCTGA TGGAGCCAAA CCCAGTGGAA 
GATGTGCAAT GCTTATCAGA GTCAAGGAGC CTTTACTTGA ACTGGACCAG CTCTCCTGGA 
GATGTGGAGG CTTATGAAGT GGTGACAGAG AGACTTTCTG ATGGACTTCC CACCTCCAAG 
TATGTCATGA GCATCCCTAT GAGTGAGGCC AGCCTGGAGG GGCTGCAGCC AAACTCGTCA 
TACCGAATCG CTGTGAGCAC AGTGGGCATG AACACAATTA GGAGCCAGGC TGTGACTCTG 
CTCTGCAACA CAACAGTGGA GGCCTTGCCT CCGCCCCTGC GAGCAGACAT CTTCCAAGTA 
GAGGCCAGCT CCGCAGTTAT CATTTCATCC GACCTGTTCA GTGAAGAGAA TGGCCAGATT 
GAGTATTACG GTGTCGTTGC TACCACTAAT GATTCACTGC TGAGGCCCAC CCAGGAAATC 
ATGTCCAGCA CATGGTATGA CCACTATTAC GGGACAGAGG ACTCCTACCT GGCTGTGCTA 
ATCCCCAATC CTTTCCATTC GAGCCCCAGG AGCTCCCCCG AAACCTGGCG AGTGCCAGTG 
GGAATAGAGG AGTGCGGCCA GTCCAGGGCA ATGTGCAACG GGAAGCTGAA AGCCAACGAG 
CAGTACAGGT TCAGCATTGC TGCCTTCACC AAATACGACC CAGTAGCCCC TGCAGTGACC 
TTCACCATGT TCTCAGCTGC TGAATCCAGT GCAGATGCAA CTCCACTCTC AATGCCAATA 
ATCACTGGAA TCATCGTGGG ATTTCTCTTG ACACTTGCAG CTATTTTTGC TTTGGTCTAC 
TGGAAACAGC TCAGAGCAAA GAGAACCAAC AAAAGCAGCC TGCCCCAGGA AATGGTGACC 
TACAGCTTGA GAAACATCCA TCGGCCAATT CCCATACAGA ACTTCAAGCA GTACTATGAG 
TTGAAGACAG CAAGTGCTAA CCACGCTTTT TTCCAGGAGT TTGAGGAGCT GAAGGAAGTT 
GGGAAGGAGC AGCCAAAGGT GGAGGCTGAG CTACCAGCAA ATGTCTCCAA GAACAGATAT 
CCCCACGTGC TGCCCTATGA TCACTCTCGG GTCAAGCTGA GCCAACTGGG GGAGGATCCA 
CACTCTGACT ACATCAATGC CAACTTCATG CCTGGCTATA CATCCCAGCA GGAGTTCATT 
GCCACCCAGG GGCCCCTGAA GAAAACGATA GAGGACTTCT GGAGGCTGGT GTGGGAGCAG 
AATGTCTGCA ACATCATCAT GCTGACAGTA TGCATGGAGA ACGGGCGGGT TCTCTGTGAT 
CACTACTGGC CATCCGAATC TGCCCCAGTC TCCTATGGGC ACATCCGTGT CCACTTGCTG 
TCACAGAGCA GCTCCGAGGA GTGGACGATG CGCGAGTTCA AACTGTGGCA TGAGGGTATC 
CGGGCAGAGC GGCATGTGTC CCACCTGCAC TACACGGCAT GGCCTGACCA TGGGATCCCA 
GAGTCCACGA CTTCCATTAT GACCTTCAGA GAGCTGGTCC GGGAGCGTAT CCAGAGCACC 
AAGGACGCGG GGCCAACCCT CGTGCACTGC AGCGCTGGGG TGGGCCGCAC TGGCACCTTC 
ATTGCACTGG ACCGGCTGCT GCAGCAGATG AAGCAGGAGA AGGTGGTGGA CACGTTTGGT 
GTTGTTTACA CCTTGCGGAT GAACCGTTAC CTGATGATTC AGACGCTGTC CCAGTATATT 
TTCCTGCACA GCTGTATTCT GGAAAAGATC TTAGAGGAAC CACTCCTGGG TTTATCAGGG 
ACAGAGAGGT CCTGCCCCAT CCCGCTGAAA AGCTTTGCTC AGCACTATGC CCAGAAGGCA 
ACCAAGTCGC ATGTGGGCTT CCTGAGGGAG TACGAGACCC TCCTAGAGGC TGTCAAGGAA 
GAAGCCAGCT CTGCAACACC ATCTTCAGGC GACCAGCAGA CACATCCCTC TTCCAGTATC 
CTCCCATATG ATCGCTCCAG AGTGAAGTTT TCCCTGCTGG AACAGGGCCC ATTCTCAGGT 
CTCCTGCAGG TGTGGCGTGT CCCGGGCTGC AGCTCCAGCA GGGAATATCT GGCTGTCCAG 
GGGCCTGACA AGTTGACTGT GGAGGACTTC TGGACCCTGG TGTGGGAACA GGACGTTCAC 
ACCATCCTAA CACTTCTCCC ATGGCAGGAG AAGGGGGAGA TCCCAAGTGG GGCGTGCTGG 
CCCCTGGAAG GAGACTCTCT CTGCACAAAG ACGCTGACCA TCCAGTGTGG CACAGAGAAG 
CTTGTCTCGG GATGGAGGTG CATCCAGCTC AAAATGAAAC ATGAGAAGAA AGCAAAGGAG 
AGGCAGGTAC AACAGTTCCT ATACACACTG TGGAGCAGCA AGAAACAGCC AGATGTCCAA 
AGCCTGGTGG AGTTCCTCAC TGCTGTCAGA CGGTGCATGC CCCACCGAAA GAGAGCCGGT 
CCTCTCCTGC TGCACTGCAG CAGTGGCGTG AGCCAAATGG GGACACTGAT CTCCCTGGAC 
TGCCTGTTGC ACCAGATGAA AGCTGAGAGA ACGGTGGATG TCTATGGGGT CACCCTCCAG 
CTGGCAAGAA GCTGTTGTCT CATGACCCCA ACACTGGACC AGTACATGTT CCTGTACACC 
TGCATCCGGG ACATCATTGC TCAGAAACAG CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801831.1
CDS	422..7117
Translation	MRPPPPLLLLVLWVLRLLGTLAEDEGCNQTARAGLEGQDAPNDRGTLLNLSVSDHGWSDSLLLSWDEPEGGAKGYSLALSSLGSGTPLQNGSAGPNITSFWFHGLTPGMRYEIEVTATLACMETTSQTVTAQTSPSPVRNLTLSSGGSSAMLQASWAHAHGQRDSYHLALYHSDSQTLVRNVSILPNASTFLFDGLLPGSEYALKVSTLVGSSQASTSIHQWTAPSIPTQLRLSPGSSTSLIASWADAGGAAWLHLTLHNLLTQMVTTTLSARRGLTSYTFQHLHSGTQYWLGLSAMAGPYTMVGPNATAWTYPLSPGNVTLSPAEEQNSLQARWSTPAGERDFYLVTLQEEEDGVPTRNISVDGDNSHVTFHGLDPGKQYSVQVTAVAGPYRASAHSTAAWTQPLAPSGVSLSSQGSPYSLLASWEEAMGEGYVLALSPMEHPVKNSSLVKGVTNFTYNGLHPGTLYTFEVSTVAGPYTSSPRRITNWTYPLPLEQLTLSNQGHSTSLQASWRAAPAGSTGYAGTLWETKSQERVRNMTVGNNWMNATFEDLVPGRQYTLEMTAMAGPYRSPVRSATDWTYPLAPAGVTLTNTRRPLGLAAFWDKAAGDVDQFHLQLYSKSHPAQRNISVGPDTHNFTFLGLSPGIQYFLKVTVLAGPYRSSSHFATEWTYPLSLANVSVQPGRRPQELHVSWVESGGGRDHLVQLSVAESLSIIKNVSVPRGVNQLDLEGLVPGSRYRVEIISQAGPHRTSSQTAIGYTVPLPPLSLSASPVSTAWALAVHWETPSGQRDGYLLSVHEEGSSKPARNLKVGKDSTNVTLAQLEPGTCYLVEIWAVAGPYRSLPKNFTGCTVPAAPTDLSLTNPGSSSELYTCWNKPPGRRDRYHVILYGLSIQSRNRVQTLSPDAQNITWTHLEAGSRFAVQVTAVKGSLEASSTNVTQWTYPLAPANLTLGSPSASELQASWATTGRGAEGYVVDVYDRASSSHVGHVVLGGDARSHTFRKLSPGTRYSVAVRATAGPFHTSTPNFTLCTRPLPPAAVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFMGLNPGYKYSLEASAMAGPYHAAAPNISAWTRPLPPASVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFTGLNPGSKYVLEAGSIAGSYQTPAGNISNWTCILTANVSVEKGTSKFTFSGLAPGHKYLLEVVSTAGPYAASAGNISDWTTPSVPKNLSAMAEGNNTVLISWDSVSGQQDECQLWLRDPRNSTLPWRHALSQGQVHHLLQGLVPGRNYSVSLSCVAGPYWSSTKPLAVLMEPNPVEDVQCLSESRSLYLNWTSSPGDVEAYEVVTERLSDGLPTSKYVMSIPMSEASLEGLQPNSSYRIAVSTVGMNTIRSQAVTLLCNTTVEALPPPLRADIFQVEASSAVIISSDLFSEENGQIEYYGVVATTNDSLLRPTQEIMSSTWYDHYYGTEDSYLAVLIPNPFHSSPRSSPETWRVPVGIEECGQSRAMCNGKLKANEQYRFSIAAFTKYDPVAPAVTFTMFSAAESSADATPLSMPIITGIIVGFLLTLAAIFALVYWKQLRAKRTNKSSLPQEMVTYSLRNIHRPIPIQNFKQYYELKTASANHAFFQEFEELKEVGKEQPKVEAELPANVSKNRYPHVLPYDHSRVKLSQLGEDPHSDYINANFMPGYTSQQEFIATQGPLKKTIEDFWRLVWEQNVCNIIMLTVCMENGRVLCDHYWPSESAPVSYGHIRVHLLSQSSSEEWTMREFKLWHEGIRAERHVSHLHYTAWPDHGIPESTTSIMTFRELVRERIQSTKDAGPTLVHCSAGVGRTGTFIALDRLLQQMKQEKVVDTFGVVYTLRMNRYLMIQTLSQYIFLHSCILEKILEEPLLGLSGTERSCPIPLKSFAQHYAQKATKSHVGFLREYETLLEAVKEEASSATPSSGDQQTHPSSSILPYDRSRVKFSLLEQGPFSGLLQVWRVPGCSSSREYLAVQGPDKLTVEDFWTLVWEQDVHTILTLLPWQEKGEIPSGACWPLEGDSLCTKTLTIQCGTEKLVSGWRCIQLKMKHEKKAKERQVQQFLYTLWSSKKQPDVQSLVEFLTAVRRCMPHRKRAGPLLLHCSSGVSQMGTLISLDCLLHQMKAERTVDVYGVTLQLARSCCLMTPTLDQYMFLYTCIRDIIAQKQP

Target ORF information:

RefSeq Version	XM_014946345.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946345.1

ORF Insert Sequence:

ATGCGACCGC CGCCGCCGCT GCTGCTGCTC GTCCTGTGGG TGCTGCGGCT CCTGGGAACC 
CTGGCAGAGG ATGAGGGATG CAACCAGACA GCACGGGCAG GGTTGGAGGG ACAGGATGCA 
CCCAATGATA GAGGGACCCT CCTGAACCTC AGTGTGAGTG ACCATGGCTG GTCGGACTCC 
CTCCTTCTGT CCTGGGATGA GCCAGAGGGG GGTGCCAAAG GATATTCGCT CGCTCTCTCC 
TCCCTGGGGT CAGGCACACC GTTGCAGAAT GGATCTGCTG GACCCAACAT CACCAGCTTC 
TGGTTCCATG GGCTGACCCC TGGCATGCGC TATGAGATTG AGGTGACAGC CACACTTGCC 
TGCATGGAGA CCACCAGCCA GACAGTCACA GCACAGACAA GTCCTTCACC TGTCCGCAAC 
CTGACCCTGA GCAGTGGTGG GAGCTCTGCC ATGCTGCAGG CGTCCTGGGC GCATGCCCAC 
GGGCAGCGAG ACAGCTACCA CCTAGCCCTC TACCACAGCG ACTCCCAGAC GCTGGTGAGA 
AATGTGTCCA TCCTGCCAAA CGCCTCTACC TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT 
GAGTACGCCT TGAAGGTCAG CACGCTGGTT GGATCCAGTC AGGCGAGCAC CAGTATCCAC 
CAGTGGACAG CTCCCTCCAT CCCCACCCAG CTGAGACTCA GCCCAGGATC CAGCACCAGC 
CTCATCGCCT CCTGGGCAGA TGCTGGGGGG GCTGCCTGGC TGCACCTCAC ACTCCACAAC 
CTCCTCACCC AGATGGTGAC CACCACCCTG TCAGCCAGAA GGGGCCTCAC CAGCTACACC 
TTCCAGCATC TCCACTCCGG CACCCAGTAC TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC 
TACACCATGG TGGGGCCCAA TGCCACCGCT TGGACATACC CCCTGAGCCC TGGAAATGTG 
ACCCTGAGCC CTGCAGAGGA ACAGAACTCC TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA 
GAGAGGGACT TCTACCTGGT GACACTGCAG GAGGAAGAGG ATGGTGTGCC AACAAGGAAC 
ATCTCTGTCG ATGGAGACAA CAGTCATGTC ACTTTCCACG GGCTGGATCC TGGGAAGCAA 
TACTCTGTCC AGGTGACAGC AGTGGCTGGG CCTTACAGAG CATCAGCCCA CAGCACAGCA 
GCCTGGACAC AGCCGCTAGC ACCATCTGGT GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC 
AGCCTACTGG CCAGCTGGGA GGAGGCAATG GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC 
ATGGAGCACC CTGTGAAGAA TAGCTCGCTG GTTAAAGGTG TCACCAACTT CACCTACAAC 
GGCCTCCACC CTGGGACTCT TTACACCTTT GAGGTCAGCA CCGTGGCTGG CCCCTACACA 
TCCTCGCCCC GGCGCATCAC CAACTGGACA TATCCTTTGC CCCTGGAGCA GCTGACCCTC 
AGCAATCAGG GCCACAGCAC CTCTCTGCAA GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC 
ACTGGCTACG CAGGCACCCT CTGGGAAACC AAGTCCCAGG AGCGGGTCAG GAACATGACT 
GTGGGGAACA ACTGGATGAA TGCCACCTTT GAGGACCTGG TCCCTGGGCG GCAGTATACA 
CTGGAGATGA CTGCTATGGC CGGACCTTAC AGATCCCCTG TGCGATCAGC CACAGACTGG 
ACATACCCTC TGGCTCCAGC CGGCGTGACC CTGACCAACA CCCGACGCCC ACTGGGACTT 
GCTGCCTTCT GGGACAAGGC TGCTGGTGAT GTGGACCAGT TCCACCTCCA GCTCTACAGC 
AAGAGCCATC CAGCACAGAG GAACATCTCA GTAGGGCCAG ACACCCACAA CTTCACATTC 
CTAGGGCTGT CCCCTGGGAT TCAGTACTTC CTGAAGGTGA CTGTCCTCGC TGGCCCATAC 
AGATCCTCGT CACACTTTGC CACTGAGTGG ACATATCCAC TGTCTCTGGC TAACGTGAGT 
GTACAGCCTG GCCGGAGACC TCAGGAGCTA CATGTGAGCT GGGTGGAGTC AGGTGGTGGC 
AGAGACCACT TGGTACAGCT CTCAGTGGCC GAGTCCCTAT CCATCATAAA GAATGTGTCT 
GTCCCCCGTG GAGTCAACCA GCTTGACCTG GAGGGGCTGG TTCCAGGGTC TCGATACCGT 
GTGGAGATCA TTTCTCAGGC TGGGCCTCAT CGCACCTCCT CTCAGACTGC CATTGGCTAC 
ACTGTCCCAC TACCTCCGCT TTCCCTCTCG GCAAGCCCTG TCAGCACTGC CTGGGCTCTG 
GCTGTGCACT GGGAGACCCC TTCTGGGCAG AGGGATGGAT ATCTGCTCAG TGTGCATGAG 
GAAGGTTCTT CTAAACCAGC AAGGAACCTG AAAGTAGGGA AAGACAGCAC CAATGTCACC 
CTGGCACAGC TGGAACCAGG AACTTGCTAC CTTGTTGAGA TCTGGGCTGT AGCTGGACCT 
TACCGCTCAC TCCCCAAGAA CTTCACTGGC TGCACAGTTC CTGCTGCACC GACAGACCTG 
AGCCTCACCA ACCCAGGCAG CTCCTCAGAG CTTTACACAT GCTGGAACAA GCCCCCCGGC 
AGGAGGGATC GCTACCATGT TATCCTGTAT GGCCTCAGCA TCCAGAGTAG GAATCGGGTC 
CAGACCTTGA GTCCAGATGC CCAGAACATC ACCTGGACTC ACCTGGAGGC AGGCAGCAGG 
TTTGCTGTGC AGGTCACTGC TGTGAAAGGT TCATTAGAAG CCTCCTCCAC CAACGTCACC 
CAATGGACAT ATCCCTTGGC CCCTGCCAAC CTCACCCTGG GCAGCCCTTC TGCCTCCGAG 
CTGCAGGCCT CCTGGGCAAC AACGGGGCGA GGAGCAGAAG GCTATGTGGT GGATGTCTAT 
GATAGAGCCT CCAGCAGTCA CGTTGGGCAC GTGGTGCTGG GTGGGGATGC CCGGAGTCAC 
ACCTTCAGAA AGCTGAGCCC TGGTACTCGC TACAGCGTGG CAGTGAGGGC CACAGCTGGG 
CCCTTCCACA CCAGCACCCC CAACTTCACC CTCTGCACAC GCCCGCTGCC CCCAGCCGCC 
GTGCGCTTGC TGAGCACGGG GCACCCCGAC AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT 
GGGGGGCGAG ACGGCTACAC ACTGACCCTG TACCACGGGC GGCTGGGCAG CATGGTGGCT 
ACCACCTCTC TTGGGAGAGA CACCCACAAC TTCACCTTCA TGGGTCTGAA CCCAGGATAC 
AAGTACTCCC TGGAGGCCAG TGCCATGGCT GGACCATACC ACGCAGCAGC ACCCAACATC 
AGTGCCTGGA CACGCCCGCT GCCCCCAGCC TCCGTGCGCT TGCTGAGCAC GGGGCACCCC 
GACAGGCTGA GTGCGTCCTG GGCAGCCGCG GCTGGGGGGC GAGACGGCTA CACACTGACC 
CTGTACCACG GGCGGCTGGG CAGCATGGTG GCTACCACCT CTCTTGGGAG AGACACCCAC 
AACTTCACCT TCACGGGCCT GAACCCAGGA AGCAAGTATG TTCTGGAGGC GGGATCCATA 
GCAGGGTCCT ACCAGACACC TGCAGGGAAC ATCAGCAACT GGACGTGTAT TCTGACAGCC 
AATGTATCTG TTGAGAAAGG CACCAGCAAA TTCACCTTTT CTGGCCTGGC TCCGGGACAT 
AAATACCTGC TGGAAGTGGT GTCCACGGCA GGGCCCTACG CAGCCTCTGC AGGGAACATC 
AGTGACTGGA CAACCCCCTC AGTTCCCAAA AATCTCTCTG CGATGGCTGA AGGGAACAAC 
ACAGTGCTCA TCTCCTGGGA CAGTGTCTCT GGACAACAGG ATGAGTGCCA GTTGTGGCTG 
CGGGATCCCA GGAACAGCAC ACTGCCCTGG CGGCACGCTC TCAGCCAAGG GCAAGTCCAC 
CACCTCCTCC AGGGGCTGGT CCCAGGGAGG AACTACTCTG TCTCCTTGAG CTGCGTGGCC 
GGGCCCTACT GGAGCAGCAC CAAACCCTTG GCGGTGCTGA TGGAGCCAAA CCCAGTGGAA 
GATGTGCAAT GCTTATCAGA GTCAAGGAGC CTTTACTTGA ACTGGACCAG CTCTCCTGGA 
GATGTGGAGG CTTATGAAGT GGTGACAGAG AGACTTTCTG ATGGACTTCC CACCTCCAAG 
TATGTCATGA GCATCCCTAT GAGTGAGGCC AGCCTGGAGG GGCTGCAGCC AAACTCGTCA 
TACCGAATCG CTGTGAGCAC AGTGGGCATG AACACAATTA GGAGCCAGGC TGTGACTCTG 
CTCTGCAACA CAACAGTGGA GGCCTTGCCT CCGCCCCTGC GAGCAGACAT CTTCCAAGTA 
GAGGCCAGCT CCGCAGTTAT CATTTCATCC GACCTGTTCA GTGAAGAGAA TGGCCAGATT 
GAGTATTACG GTGTCGTTGC TACCACTAAT GATTCACTGC TGAGGCCCAC CCAGGAAATC 
ATGTCCAGCA CATGGTATGA CCACTATTAC GGGACAGAGG ACTCCTACCT GGCTGTGCTA 
ATCCCCAATC CTTTCCATTC GAGCCCCAGG AGCTCCCCCG AAACCTGGCG AGTGCCAGTG 
GGAATAGAGG AGTGCGGCCA GTCCAGGGCA ATGTGCAACG GGAAGCTGAA AGCCAACGAG 
CAGTACAGGT TCAGCATTGC TGCCTTCACC AAATACGACC CAGTAGCCCC TGCAGTGACC 
TTCACCATGT TCTCAGCTGC TGAATCCAGT GCAGATGCAA CTCCACTCTC AATGCCAATA 
ATCACTGGAA TCATCGTGGG ATTTCTCTTG ACACTTGCAG CTATTTTTGC TTTGGTCTAC 
TGGAAACAGC TCAGAGCAAA GAGAACCAAC AAAAGCAGCC TGCCCCAGGA AATGGTGACC 
TACAGCTTGA GAAACATCCA TCGGCCAATT CCCATACAGA ACTTCAAGCA GTACTATGAG 
TTGAAGACAG CAAGTGCTAA CCACGCTTTT TTCCAGGAGT TTGAGGAGCT GAAGGAAGTT 
GGGAAGGAGC AGCCAAAGGT GGAGGCTGAG CTACCAGCAA ATGTCTCCAA GAACAGATAT 
CCCCACGTGC TGCCCTATGA TCACTCTCGG GTCAAGCTGA GCCAACTGGG GGAGGATCCA 
CACTCTGACT ACATCAATGC CAACTTCATG CCTGGCTATA CATCCCAGCA GGAGTTCATT 
GCCACCCAGG GGCCCCTGAA GAAAACGATA GAGGACTTCT GGAGGCTGGT GTGGGAGCAG 
AATGTCTGCA ACATCATCAT GCTGACAGTA TGCATGGAGA ACGGGCGGGT TCTCTGTGAT 
CACTACTGGC CATCCGAATC TGCCCCAGTC TCCTATGGGC ACATCCGTGT CCACTTGCTG 
TCACAGAGCA GCTCCGAGGA GTGGACGATG CGCGAGTTCA AACTGTGGCA TGAGGGTATC 
CGGGCAGAGC GGCATGTGTC CCACCTGCAC TACACGGCAT GGCCTGACCA TGGGATCCCA 
GAGTCCACGA CTTCCATTAT GACCTTCAGA GAGCTGGTCC GGGAGCGTAT CCAGAGCACC 
AAGGACGCGG GGCCAACCCT CGTGCACTGC AGCGCTGGGG TGGGCCGCAC TGGCACCTTC 
ATTGCACTGG ACCGGCTGCT GCAGCAGATG AAGCAGGAGA AGGTGGTGGA CACGTTTGGT 
GTTGTTTACA CCTTGCGGAT GAACCGTTAC CTGATGATTC AGACGCTGTC CCAGTATATT 
TTCCTGCACA GCTGTATTCT GGAAAAGATC TTAGAGGAAC CACTCCTGGG TTTATCAGGG 
ACAGAGAGGT CCTGCCCCAT CCCGCTGAAA AGCTTTGCTC AGCACTATGC CCAGAAGGCA 
ACCAAGTCGC ATGTGGGCTT CCTGAGGGAG TACGAGACCC TCCTAGAGGC TGTCAAGGAA 
GAAGCCAGCT CTGCAACACC ATCTTCAGGC GACCAGCAGA CACATCCCTC TTCCAGTATC 
CTCCCATATG ATCGCTCCAG AGTGAAGTTT TCCCTGCTGG AACAGGGCCC ATTCTCAGGT 
CTCCTGCAGG TGTGGCGTGT CCCGGGCTGC AGCTCCAGCA GGGAATATCT GGCTGTCCAG 
GGGCCTGACA AGTTGACTGT GGAGGACTTC TGGACCCTGG TGTGGGAACA GGACGTTCAC 
ACCATCCTAA CACTTCTCCC ATGGCAGGAG AAGGGGGAGA TCCCAAGTGG GGCGTGCTGG 
CCCCTGGAAG GAGACTCTCT CTGCACAAAG ACGCTGACCA TCCAGTGTGG CACAGAGAAG 
CTTGTCTCGG GATGGAGGTG CATCCAGCTC AAAATGAAAC ATGAGAAGAA AGCAAAGGAG 
AGGCAGGTAC AACAGTTCCT ATACACACTG TGGAGCAGCA AGAAACAGCC AGATGTCCAA 
AGCCTGGTGG AGTTCCTCAC TGCTGTCAGA CGGTGCATGC CCCACCGAAA GAGAGCCGGT 
CCTCTCCTGC TGCACTGCAG CAGTGGCGTG AGCCAAATGG GGACACTGAT CTCCCTGGAC 
TGCCTGTTGC ACCAGATGAA AGCTGAGAGA ACGGTGGATG TCTATGGGGT CACCCTCCAG 
CTGGCAAGAA GCTGTTGTCT CATGACCCCA ACACTGGACC AGTACATGTT CCTGTACACC 
TGCATCCGGG ACATCATTGC TCAGAAACAG CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106890484 ( XM_014946347.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801833.1 Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X5, mRNA.

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CloneID	OCa68223
Clone ID Related Accession (Same CDS sequence)	XM_014946347.1
Accession Version	XM_014946347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	receptor-type tyrosine-protein phosphatase V-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTATG AGATTGAGGT GACAGCCACA CTTGCCTGCA TGGAGACCAC CAGCCAGACA 
GTCACAGCAC AGACAAGTCC TTCACCTGTC CGCAACCTGA CCCTGAGCAG TGGTGGGAGC 
TCTGCCATGC TGCAGGCGTC CTGGGCGCAT GCCCACGGGC AGCGAGACAG CTACCACCTA 
GCCCTCTACC ACAGCGACTC CCAGACGCTG GTGAGAAATG TGTCCATCCT GCCAAACGCC 
TCTACCTTCC TGTTTGACGG GTTGCTACCT GGCAGTGAGT ACGCCTTGAA GGTCAGCACG 
CTGGTTGGAT CCAGTCAGGC GAGCACCAGT ATCCACCAGT GGACAGCTCC CTCCATCCCC 
ACCCAGCTGA GACTCAGCCC AGGATCCAGC ACCAGCCTCA TCGCCTCCTG GGCAGATGCT 
GGGGGGGCTG CCTGGCTGCA CCTCACACTC CACAACCTCC TCACCCAGAT GGTGACCACC 
ACCCTGTCAG CCAGAAGGGG CCTCACCAGC TACACCTTCC AGCATCTCCA CTCCGGCACC 
CAGTACTGGT TGGGGCTGAG TGCCATGGCT GGGCCCTACA CCATGGTGGG GCCCAATGCC 
ACCGCTTGGA CATACCCCCT GAGCCCTGGA AATGTGACCC TGAGCCCTGC AGAGGAACAG 
AACTCCTTGC AGGCTCGCTG GAGCACCCCA GCGGGAGAGA GGGACTTCTA CCTGGTGACA 
CTGCAGGAGG AAGAGGATGG TGTGCCAACA AGGAACATCT CTGTCGATGG AGACAACAGT 
CATGTCACTT TCCACGGGCT GGATCCTGGG AAGCAATACT CTGTCCAGGT GACAGCAGTG 
GCTGGGCCTT ACAGAGCATC AGCCCACAGC ACAGCAGCCT GGACACAGCC GCTAGCACCA 
TCTGGTGTGA GTCTGTCCAG CCAAGGGAGC CCCTACAGCC TACTGGCCAG CTGGGAGGAG 
GCAATGGGAG AGGGCTACGT GCTGGCCCTC AGCCCCATGG AGCACCCTGT GAAGAATAGC 
TCGCTGGTTA AAGGTGTCAC CAACTTCACC TACAACGGCC TCCACCCTGG GACTCTTTAC 
ACCTTTGAGG TCAGCACCGT GGCTGGCCCC TACACATCCT CGCCCCGGCG CATCACCAAC 
TGGACATATC CTTTGCCCCT GGAGCAGCTG ACCCTCAGCA ATCAGGGCCA CAGCACCTCT 
CTGCAAGCCT CCTGGAGAGC AGCTCCCGCT GGCAGCACTG GCTACGCAGG CACCCTCTGG 
GAAACCAAGT CCCAGGAGCG GGTCAGGAAC ATGACTGTGG GGAACAACTG GATGAATGCC 
ACCTTTGAGG ACCTGGTCCC TGGGCGGCAG TATACACTGG AGATGACTGC TATGGCCGGA 
CCTTACAGAT CCCCTGTGCG ATCAGCCACA GACTGGACAT ACCCTCTGGC TCCAGCCGGC 
GTGACCCTGA CCAACACCCG ACGCCCACTG GGACTTGCTG CCTTCTGGGA CAAGGCTGCT 
GGTGATGTGG ACCAGTTCCA CCTCCAGCTC TACAGCAAGA GCCATCCAGC ACAGAGGAAC 
ATCTCAGTAG GGCCAGACAC CCACAACTTC ACATTCCTAG GGCTGTCCCC TGGGATTCAG 
TACTTCCTGA AGGTGACTGT CCTCGCTGGC CCATACAGAT CCTCGTCACA CTTTGCCACT 
GAGTGGACAT ATCCACTGTC TCTGGCTAAC GTGAGTGTAC AGCCTGGCCG GAGACCTCAG 
GAGCTACATG TGAGCTGGGT GGAGTCAGGT GGTGGCAGAG ACCACTTGGT ACAGCTCTCA 
GTGGCCGAGT CCCTATCCAT CATAAAGAAT GTGTCTGTCC CCCGTGGAGT CAACCAGCTT 
GACCTGGAGG GGCTGGTTCC AGGGTCTCGA TACCGTGTGG AGATCATTTC TCAGGCTGGG 
CCTCATCGCA CCTCCTCTCA GACTGCCATT GGCTACACTG TCCCACTACC TCCGCTTTCC 
CTCTCGGCAA GCCCTGTCAG CACTGCCTGG GCTCTGGCTG TGCACTGGGA GACCCCTTCT 
GGGCAGAGGG ATGGATATCT GCTCAGTGTG CATGAGGAAG GTTCTTCTAA ACCAGCAAGG 
AACCTGAAAG TAGGGAAAGA CAGCACCAAT GTCACCCTGG CACAGCTGGA ACCAGGAACT 
TGCTACCTTG TTGAGATCTG GGCTGTAGCT GGACCTTACC GCTCACTCCC CAAGAACTTC 
ACTGGCTGCA CAGTTCCTGC TGCACCGACA GACCTGAGCC TCACCAACCC AGGCAGCTCC 
TCAGAGCTTT ACACATGCTG GAACAAGCCC CCCGGCAGGA GGGATCGCTA CCATGTTATC 
CTGTATGGCC TCAGCATCCA GAGTAGGAAT CGGGTCCAGA CCTTGAGTCC AGATGCCCAG 
AACATCACCT GGACTCACCT GGAGGCAGGC AGCAGGTTTG CTGTGCAGGT CACTGCTGTG 
AAAGGTTCAT TAGAAGCCTC CTCCACCAAC GTCACCCAAT GGACATATCC CTTGGCCCCT 
GCCAACCTCA CCCTGGGCAG CCCTTCTGCC TCCGAGCTGC AGGCCTCCTG GGCAACAACG 
GGGCGAGGAG CAGAAGGCTA TGTGGTGGAT GTCTATGATA GAGCCTCCAG CAGTCACGTT 
GGGCACGTGG TGCTGGGTGG GGATGCCCGG AGTCACACCT TCAGAAAGCT GAGCCCTGGT 
ACTCGCTACA GCGTGGCAGT GAGGGCCACA GCTGGGCCCT TCCACACCAG CACCCCCAAC 
TTCACCCTCT GCACACGCCC GCTGCCCCCA GCCGCCGTGC GCTTGCTGAG CACGGGGCAC 
CCCGACAGGC TGAGTGCGTC CTGGGCAGCC GCGGCTGGGG GGCGAGACGG CTACACACTG 
ACCCTGTACC ACGGGCGGCT GGGCAGCATG GTGGCTACCA CCTCTCTTGG GAGAGACACC 
CACAACTTCA CCTTCATGGG TCTGAACCCA GGATACAAGT ACTCCCTGGA GGCCAGTGCC 
ATGGCTGGAC CATACCACGC AGCAGCACCC AACATCAGTG CCTGGACACG CCCGCTGCCC 
CCAGCCTCCG TGCGCTTGCT GAGCACGGGG CACCCCGACA GGCTGAGTGC GTCCTGGGCA 
GCCGCGGCTG GGGGGCGAGA CGGCTACACA CTGACCCTGT ACCACGGGCG GCTGGGCAGC 
ATGGTGGCTA CCACCTCTCT TGGGAGAGAC ACCCACAACT TCACCTTCAC GGGCCTGAAC 
CCAGGAAGCA AGTATGTTCT GGAGGCGGGA TCCATAGCAG GGTCCTACCA GACACCTGCA 
GGGAACATCA GCAACTGGAC GTACCCCCTG GCACCTCGTA ACGTGTACAT GACGAACCAG 
GGGTATCCCA ACAGGCTGAG TGTGTCCTGG CGAGCTGAAC CCCAAGGGCA AGACAGTTAC 
AGGCTCCTCC TGTATCATTC GGGATCAGGT ATTCTGACAG CCAATGTATC TGTTGAGAAA 
GGCACCAGCA AATTCACCTT TTCTGGCCTG GCTCCGGGAC ATAAATACCT GCTGGAAGTG 
GTGTCCACGG CAGGGCCCTA CGCAGCCTCT GCAGGGAACA TCAGTGACTG GACAACCCCC 
TCAGTTCCCA AAAATCTCTC TGCGATGGCT GAAGGGAACA ACACAGTGCT CATCTCCTGG 
GACAGTGTCT CTGGACAACA GGATGAGTGC CAGTTGTGGC TGCGGGATCC CAGGAACAGC 
ACACTGCCCT GGCGGCACGC TCTCAGCCAA GGGCAAGTCC ACCACCTCCT CCAGGGGCTG 
GTCCCAGGGA GGAACTACTC TGTCTCCTTG AGCTGCGTGG CCGGGCCCTA CTGGAGCAGC 
ACCAAACCCT TGGCGGTGCT GATGGAGCCA AACCCAGTGG AAGATGTGCA ATGCTTATCA 
GAGTCAAGGA GCCTTTACTT GAACTGGACC AGCTCTCCTG GAGATGTGGA GGCTTATGAA 
GTGGTGACAG AGAGACTTTC TGATGGACTT CCCACCTCCA AGTATGTCAT GAGCATCCCT 
ATGAGTGAGG CCAGCCTGGA GGGGCTGCAG CCAAACTCGT CATACCGAAT CGCTGTGAGC 
ACAGTGGGCA TGAACACAAT TAGGAGCCAG GCTGTGACTC TGCTCTGCAA CACAACAGTG 
GAGGCCTTGC CTCCGCCCCT GCGAGCAGAC ATCTTCCAAG TAGAGGCCAG CTCCGCAGTT 
ATCATTTCAT CCGACCTGTT CAGTGAAGAG AATGGCCAGA TTGAGTATTA CGGTGTCGTT 
GCTACCACTA ATGATTCACT GCTGAGGCCC ACCCAGGAAA TCATGTCCAG CACATGGTAT 
GACCACTATT ACGGGACAGA GGACTCCTAC CTGGCTGTGC TAATCCCCAA TCCTTTCCAT 
TCGAGCCCCA GGAGCTCCCC CGAAACCTGG CGAGTGCCAG TGGGAATAGA GGAGTGCGGC 
CAGTCCAGGG CAATGTGCAA CGGGAAGCTG AAAGCCAACG AGCAGTACAG GTTCAGCATT 
GCTGCCTTCA CCAAATACGA CCCAGTAGCC CCTGCAGTGA CCTTCACCAT GTTCTCAGCT 
GCTGAATCCA GTGCAGATGC AACTCCACTC TCAATGCCAA TAATCACTGG AATCATCGTG 
GGATTTCTCT TGACACTTGC AGCTATTTTT GCTTTGGTCT ACTGGAAACA GCTCAGAGCA 
AAGAGAACCA ACAAAAGCAG CCTGCCCCAG GAAATGGTGA CCTACAGCTT GAGAAACATC 
CATCGGCCAA TTCCCATACA GAACTTCAAG CAGTACTATG AGTTGAAGAC AGCAAGTGCT 
AACCACGCTT TTTTCCAGGA GTTTGAGGAG CTGAAGGAAG TTGGGAAGGA GCAGCCAAAG 
GTGGAGGCTG AGCTACCAGC AAATGTCTCC AAGAACAGAT ATCCCCACGT GCTGCCCTAT 
GATCACTCTC GGGTCAAGCT GAGCCAACTG GGGGAGGATC CACACTCTGA CTACATCAAT 
GCCAACTTCA TGCCTGGCTA TACATCCCAG CAGGAGTTCA TTGCCACCCA GGGGCCCCTG 
AAGAAAACGA TAGAGGACTT CTGGAGGCTG GTGTGGGAGC AGAATGTCTG CAACATCATC 
ATGCTGACAG TATGCATGGA GAACGGGCGG GTTCTCTGTG ATCACTACTG GCCATCCGAA 
TCTGCCCCAG TCTCCTATGG GCACATCCGT GTCCACTTGC TGTCACAGAG CAGCTCCGAG 
GAGTGGACGA TGCGCGAGTT CAAACTGTGG CATGAGGGTA TCCGGGCAGA GCGGCATGTG 
TCCCACCTGC ACTACACGGC ATGGCCTGAC CATGGGATCC CAGAGTCCAC GACTTCCATT 
ATGACCTTCA GAGAGCTGGT CCGGGAGCGT ATCCAGAGCA CCAAGGACGC GGGGCCAACC 
CTCGTGCACT GCAGCGCTGG GGTGGGCCGC ACTGGCACCT TCATTGCACT GGACCGGCTG 
CTGCAGCAGA TGAAGCAGGA GAAGGTGGTG GACACGTTTG GTGTTGTTTA CACCTTGCGG 
ATGAACCGTT ACCTGATGAT TCAGACGCTG TCCCAGTATA TTTTCCTGCA CAGCTGTATT 
CTGGAAAAGA TCTTAGAGGA ACCACTCCTG GGTTTATCAG GGACAGAGAG GTCCTGCCCC 
ATCCCGCTGA AAAGCTTTGC TCAGCACTAT GCCCAGAAGG CAACCAAGTC GCATGTGGGC 
TTCCTGAGGG AGTACGAGAC CCTCCTAGAG GCTGTCAAGG AAGAAGCCAG CTCTGCAACA 
CCATCTTCAG GCGACCAGCA GACACATCCC TCTTCCAGTA TCCTCCCATA TGATCGCTCC 
AGAGTGAAGT TTTCCCTGCT GGAACAGGGC CCATTCTCAG GTCTCCTGCA GGTGTGGCGT 
GTCCCGGGCT GCAGCTCCAG CAGGGAATAT CTGGCTGTCC AGGGGCCTGA CAAGTTGACT 
GTGGAGGACT TCTGGACCCT GGTGTGGGAA CAGGACGTTC ACACCATCCT AACACTTCTC 
CCATGGCAGG AGAAGGGGGA GATCCCAAGT GGGGCGTGCT GGCCCCTGGA AGGAGACTCT 
CTCTGCACAA AGACGCTGAC CATCCAGTGT GGCACAGAGA AGCTTGTCTC GGGATGGAGG 
TGCATCCAGC TCAAAATGAA ACATGAGAAG AAAGCAAAGG AGAGGCAGGT ACAACAGTTC 
CTATACACAC TGTGGAGCAG CAAGAAACAG CCAGATGTCC AAAGCCTGGT GGAGTTCCTC 
ACTGCTGTCA GACGGTGCAT GCCCCACCGA AAGAGAGCCG GTCCTCTCCT GCTGCACTGC 
AGCAGTGGCG TGAGCCAAAT GGGGACACTG ATCTCCCTGG ACTGCCTGTT GCACCAGATG 
AAAGCTGAGA GAACGGTGGA TGTCTATGGG GTCACCCTCC AGCTGGCAAG AAGCTGTTGT 
CTCATGACCC CAACACTGGA CCAGTACATG TTCCTGTACA CCTGCATCCG GGACATCATT 
GCTCAGAAAC AGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801833.1
CDS	188..6685
Translation	MRYEIEVTATLACMETTSQTVTAQTSPSPVRNLTLSSGGSSAMLQASWAHAHGQRDSYHLALYHSDSQTLVRNVSILPNASTFLFDGLLPGSEYALKVSTLVGSSQASTSIHQWTAPSIPTQLRLSPGSSTSLIASWADAGGAAWLHLTLHNLLTQMVTTTLSARRGLTSYTFQHLHSGTQYWLGLSAMAGPYTMVGPNATAWTYPLSPGNVTLSPAEEQNSLQARWSTPAGERDFYLVTLQEEEDGVPTRNISVDGDNSHVTFHGLDPGKQYSVQVTAVAGPYRASAHSTAAWTQPLAPSGVSLSSQGSPYSLLASWEEAMGEGYVLALSPMEHPVKNSSLVKGVTNFTYNGLHPGTLYTFEVSTVAGPYTSSPRRITNWTYPLPLEQLTLSNQGHSTSLQASWRAAPAGSTGYAGTLWETKSQERVRNMTVGNNWMNATFEDLVPGRQYTLEMTAMAGPYRSPVRSATDWTYPLAPAGVTLTNTRRPLGLAAFWDKAAGDVDQFHLQLYSKSHPAQRNISVGPDTHNFTFLGLSPGIQYFLKVTVLAGPYRSSSHFATEWTYPLSLANVSVQPGRRPQELHVSWVESGGGRDHLVQLSVAESLSIIKNVSVPRGVNQLDLEGLVPGSRYRVEIISQAGPHRTSSQTAIGYTVPLPPLSLSASPVSTAWALAVHWETPSGQRDGYLLSVHEEGSSKPARNLKVGKDSTNVTLAQLEPGTCYLVEIWAVAGPYRSLPKNFTGCTVPAAPTDLSLTNPGSSSELYTCWNKPPGRRDRYHVILYGLSIQSRNRVQTLSPDAQNITWTHLEAGSRFAVQVTAVKGSLEASSTNVTQWTYPLAPANLTLGSPSASELQASWATTGRGAEGYVVDVYDRASSSHVGHVVLGGDARSHTFRKLSPGTRYSVAVRATAGPFHTSTPNFTLCTRPLPPAAVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFMGLNPGYKYSLEASAMAGPYHAAAPNISAWTRPLPPASVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFTGLNPGSKYVLEAGSIAGSYQTPAGNISNWTYPLAPRNVYMTNQGYPNRLSVSWRAEPQGQDSYRLLLYHSGSGILTANVSVEKGTSKFTFSGLAPGHKYLLEVVSTAGPYAASAGNISDWTTPSVPKNLSAMAEGNNTVLISWDSVSGQQDECQLWLRDPRNSTLPWRHALSQGQVHHLLQGLVPGRNYSVSLSCVAGPYWSSTKPLAVLMEPNPVEDVQCLSESRSLYLNWTSSPGDVEAYEVVTERLSDGLPTSKYVMSIPMSEASLEGLQPNSSYRIAVSTVGMNTIRSQAVTLLCNTTVEALPPPLRADIFQVEASSAVIISSDLFSEENGQIEYYGVVATTNDSLLRPTQEIMSSTWYDHYYGTEDSYLAVLIPNPFHSSPRSSPETWRVPVGIEECGQSRAMCNGKLKANEQYRFSIAAFTKYDPVAPAVTFTMFSAAESSADATPLSMPIITGIIVGFLLTLAAIFALVYWKQLRAKRTNKSSLPQEMVTYSLRNIHRPIPIQNFKQYYELKTASANHAFFQEFEELKEVGKEQPKVEAELPANVSKNRYPHVLPYDHSRVKLSQLGEDPHSDYINANFMPGYTSQQEFIATQGPLKKTIEDFWRLVWEQNVCNIIMLTVCMENGRVLCDHYWPSESAPVSYGHIRVHLLSQSSSEEWTMREFKLWHEGIRAERHVSHLHYTAWPDHGIPESTTSIMTFRELVRERIQSTKDAGPTLVHCSAGVGRTGTFIALDRLLQQMKQEKVVDTFGVVYTLRMNRYLMIQTLSQYIFLHSCILEKILEEPLLGLSGTERSCPIPLKSFAQHYAQKATKSHVGFLREYETLLEAVKEEASSATPSSGDQQTHPSSSILPYDRSRVKFSLLEQGPFSGLLQVWRVPGCSSSREYLAVQGPDKLTVEDFWTLVWEQDVHTILTLLPWQEKGEIPSGACWPLEGDSLCTKTLTIQCGTEKLVSGWRCIQLKMKHEKKAKERQVQQFLYTLWSSKKQPDVQSLVEFLTAVRRCMPHRKRAGPLLLHCSSGVSQMGTLISLDCLLHQMKAERTVDVYGVTLQLARSCCLMTPTLDQYMFLYTCIRDIIAQKQP

Target ORF information:

RefSeq Version	XM_014946347.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946347.1

ORF Insert Sequence:

ATGCGCTATG AGATTGAGGT GACAGCCACA CTTGCCTGCA TGGAGACCAC CAGCCAGACA 
GTCACAGCAC AGACAAGTCC TTCACCTGTC CGCAACCTGA CCCTGAGCAG TGGTGGGAGC 
TCTGCCATGC TGCAGGCGTC CTGGGCGCAT GCCCACGGGC AGCGAGACAG CTACCACCTA 
GCCCTCTACC ACAGCGACTC CCAGACGCTG GTGAGAAATG TGTCCATCCT GCCAAACGCC 
TCTACCTTCC TGTTTGACGG GTTGCTACCT GGCAGTGAGT ACGCCTTGAA GGTCAGCACG 
CTGGTTGGAT CCAGTCAGGC GAGCACCAGT ATCCACCAGT GGACAGCTCC CTCCATCCCC 
ACCCAGCTGA GACTCAGCCC AGGATCCAGC ACCAGCCTCA TCGCCTCCTG GGCAGATGCT 
GGGGGGGCTG CCTGGCTGCA CCTCACACTC CACAACCTCC TCACCCAGAT GGTGACCACC 
ACCCTGTCAG CCAGAAGGGG CCTCACCAGC TACACCTTCC AGCATCTCCA CTCCGGCACC 
CAGTACTGGT TGGGGCTGAG TGCCATGGCT GGGCCCTACA CCATGGTGGG GCCCAATGCC 
ACCGCTTGGA CATACCCCCT GAGCCCTGGA AATGTGACCC TGAGCCCTGC AGAGGAACAG 
AACTCCTTGC AGGCTCGCTG GAGCACCCCA GCGGGAGAGA GGGACTTCTA CCTGGTGACA 
CTGCAGGAGG AAGAGGATGG TGTGCCAACA AGGAACATCT CTGTCGATGG AGACAACAGT 
CATGTCACTT TCCACGGGCT GGATCCTGGG AAGCAATACT CTGTCCAGGT GACAGCAGTG 
GCTGGGCCTT ACAGAGCATC AGCCCACAGC ACAGCAGCCT GGACACAGCC GCTAGCACCA 
TCTGGTGTGA GTCTGTCCAG CCAAGGGAGC CCCTACAGCC TACTGGCCAG CTGGGAGGAG 
GCAATGGGAG AGGGCTACGT GCTGGCCCTC AGCCCCATGG AGCACCCTGT GAAGAATAGC 
TCGCTGGTTA AAGGTGTCAC CAACTTCACC TACAACGGCC TCCACCCTGG GACTCTTTAC 
ACCTTTGAGG TCAGCACCGT GGCTGGCCCC TACACATCCT CGCCCCGGCG CATCACCAAC 
TGGACATATC CTTTGCCCCT GGAGCAGCTG ACCCTCAGCA ATCAGGGCCA CAGCACCTCT 
CTGCAAGCCT CCTGGAGAGC AGCTCCCGCT GGCAGCACTG GCTACGCAGG CACCCTCTGG 
GAAACCAAGT CCCAGGAGCG GGTCAGGAAC ATGACTGTGG GGAACAACTG GATGAATGCC 
ACCTTTGAGG ACCTGGTCCC TGGGCGGCAG TATACACTGG AGATGACTGC TATGGCCGGA 
CCTTACAGAT CCCCTGTGCG ATCAGCCACA GACTGGACAT ACCCTCTGGC TCCAGCCGGC 
GTGACCCTGA CCAACACCCG ACGCCCACTG GGACTTGCTG CCTTCTGGGA CAAGGCTGCT 
GGTGATGTGG ACCAGTTCCA CCTCCAGCTC TACAGCAAGA GCCATCCAGC ACAGAGGAAC 
ATCTCAGTAG GGCCAGACAC CCACAACTTC ACATTCCTAG GGCTGTCCCC TGGGATTCAG 
TACTTCCTGA AGGTGACTGT CCTCGCTGGC CCATACAGAT CCTCGTCACA CTTTGCCACT 
GAGTGGACAT ATCCACTGTC TCTGGCTAAC GTGAGTGTAC AGCCTGGCCG GAGACCTCAG 
GAGCTACATG TGAGCTGGGT GGAGTCAGGT GGTGGCAGAG ACCACTTGGT ACAGCTCTCA 
GTGGCCGAGT CCCTATCCAT CATAAAGAAT GTGTCTGTCC CCCGTGGAGT CAACCAGCTT 
GACCTGGAGG GGCTGGTTCC AGGGTCTCGA TACCGTGTGG AGATCATTTC TCAGGCTGGG 
CCTCATCGCA CCTCCTCTCA GACTGCCATT GGCTACACTG TCCCACTACC TCCGCTTTCC 
CTCTCGGCAA GCCCTGTCAG CACTGCCTGG GCTCTGGCTG TGCACTGGGA GACCCCTTCT 
GGGCAGAGGG ATGGATATCT GCTCAGTGTG CATGAGGAAG GTTCTTCTAA ACCAGCAAGG 
AACCTGAAAG TAGGGAAAGA CAGCACCAAT GTCACCCTGG CACAGCTGGA ACCAGGAACT 
TGCTACCTTG TTGAGATCTG GGCTGTAGCT GGACCTTACC GCTCACTCCC CAAGAACTTC 
ACTGGCTGCA CAGTTCCTGC TGCACCGACA GACCTGAGCC TCACCAACCC AGGCAGCTCC 
TCAGAGCTTT ACACATGCTG GAACAAGCCC CCCGGCAGGA GGGATCGCTA CCATGTTATC 
CTGTATGGCC TCAGCATCCA GAGTAGGAAT CGGGTCCAGA CCTTGAGTCC AGATGCCCAG 
AACATCACCT GGACTCACCT GGAGGCAGGC AGCAGGTTTG CTGTGCAGGT CACTGCTGTG 
AAAGGTTCAT TAGAAGCCTC CTCCACCAAC GTCACCCAAT GGACATATCC CTTGGCCCCT 
GCCAACCTCA CCCTGGGCAG CCCTTCTGCC TCCGAGCTGC AGGCCTCCTG GGCAACAACG 
GGGCGAGGAG CAGAAGGCTA TGTGGTGGAT GTCTATGATA GAGCCTCCAG CAGTCACGTT 
GGGCACGTGG TGCTGGGTGG GGATGCCCGG AGTCACACCT TCAGAAAGCT GAGCCCTGGT 
ACTCGCTACA GCGTGGCAGT GAGGGCCACA GCTGGGCCCT TCCACACCAG CACCCCCAAC 
TTCACCCTCT GCACACGCCC GCTGCCCCCA GCCGCCGTGC GCTTGCTGAG CACGGGGCAC 
CCCGACAGGC TGAGTGCGTC CTGGGCAGCC GCGGCTGGGG GGCGAGACGG CTACACACTG 
ACCCTGTACC ACGGGCGGCT GGGCAGCATG GTGGCTACCA CCTCTCTTGG GAGAGACACC 
CACAACTTCA CCTTCATGGG TCTGAACCCA GGATACAAGT ACTCCCTGGA GGCCAGTGCC 
ATGGCTGGAC CATACCACGC AGCAGCACCC AACATCAGTG CCTGGACACG CCCGCTGCCC 
CCAGCCTCCG TGCGCTTGCT GAGCACGGGG CACCCCGACA GGCTGAGTGC GTCCTGGGCA 
GCCGCGGCTG GGGGGCGAGA CGGCTACACA CTGACCCTGT ACCACGGGCG GCTGGGCAGC 
ATGGTGGCTA CCACCTCTCT TGGGAGAGAC ACCCACAACT TCACCTTCAC GGGCCTGAAC 
CCAGGAAGCA AGTATGTTCT GGAGGCGGGA TCCATAGCAG GGTCCTACCA GACACCTGCA 
GGGAACATCA GCAACTGGAC GTACCCCCTG GCACCTCGTA ACGTGTACAT GACGAACCAG 
GGGTATCCCA ACAGGCTGAG TGTGTCCTGG CGAGCTGAAC CCCAAGGGCA AGACAGTTAC 
AGGCTCCTCC TGTATCATTC GGGATCAGGT ATTCTGACAG CCAATGTATC TGTTGAGAAA 
GGCACCAGCA AATTCACCTT TTCTGGCCTG GCTCCGGGAC ATAAATACCT GCTGGAAGTG 
GTGTCCACGG CAGGGCCCTA CGCAGCCTCT GCAGGGAACA TCAGTGACTG GACAACCCCC 
TCAGTTCCCA AAAATCTCTC TGCGATGGCT GAAGGGAACA ACACAGTGCT CATCTCCTGG 
GACAGTGTCT CTGGACAACA GGATGAGTGC CAGTTGTGGC TGCGGGATCC CAGGAACAGC 
ACACTGCCCT GGCGGCACGC TCTCAGCCAA GGGCAAGTCC ACCACCTCCT CCAGGGGCTG 
GTCCCAGGGA GGAACTACTC TGTCTCCTTG AGCTGCGTGG CCGGGCCCTA CTGGAGCAGC 
ACCAAACCCT TGGCGGTGCT GATGGAGCCA AACCCAGTGG AAGATGTGCA ATGCTTATCA 
GAGTCAAGGA GCCTTTACTT GAACTGGACC AGCTCTCCTG GAGATGTGGA GGCTTATGAA 
GTGGTGACAG AGAGACTTTC TGATGGACTT CCCACCTCCA AGTATGTCAT GAGCATCCCT 
ATGAGTGAGG CCAGCCTGGA GGGGCTGCAG CCAAACTCGT CATACCGAAT CGCTGTGAGC 
ACAGTGGGCA TGAACACAAT TAGGAGCCAG GCTGTGACTC TGCTCTGCAA CACAACAGTG 
GAGGCCTTGC CTCCGCCCCT GCGAGCAGAC ATCTTCCAAG TAGAGGCCAG CTCCGCAGTT 
ATCATTTCAT CCGACCTGTT CAGTGAAGAG AATGGCCAGA TTGAGTATTA CGGTGTCGTT 
GCTACCACTA ATGATTCACT GCTGAGGCCC ACCCAGGAAA TCATGTCCAG CACATGGTAT 
GACCACTATT ACGGGACAGA GGACTCCTAC CTGGCTGTGC TAATCCCCAA TCCTTTCCAT 
TCGAGCCCCA GGAGCTCCCC CGAAACCTGG CGAGTGCCAG TGGGAATAGA GGAGTGCGGC 
CAGTCCAGGG CAATGTGCAA CGGGAAGCTG AAAGCCAACG AGCAGTACAG GTTCAGCATT 
GCTGCCTTCA CCAAATACGA CCCAGTAGCC CCTGCAGTGA CCTTCACCAT GTTCTCAGCT 
GCTGAATCCA GTGCAGATGC AACTCCACTC TCAATGCCAA TAATCACTGG AATCATCGTG 
GGATTTCTCT TGACACTTGC AGCTATTTTT GCTTTGGTCT ACTGGAAACA GCTCAGAGCA 
AAGAGAACCA ACAAAAGCAG CCTGCCCCAG GAAATGGTGA CCTACAGCTT GAGAAACATC 
CATCGGCCAA TTCCCATACA GAACTTCAAG CAGTACTATG AGTTGAAGAC AGCAAGTGCT 
AACCACGCTT TTTTCCAGGA GTTTGAGGAG CTGAAGGAAG TTGGGAAGGA GCAGCCAAAG 
GTGGAGGCTG AGCTACCAGC AAATGTCTCC AAGAACAGAT ATCCCCACGT GCTGCCCTAT 
GATCACTCTC GGGTCAAGCT GAGCCAACTG GGGGAGGATC CACACTCTGA CTACATCAAT 
GCCAACTTCA TGCCTGGCTA TACATCCCAG CAGGAGTTCA TTGCCACCCA GGGGCCCCTG 
AAGAAAACGA TAGAGGACTT CTGGAGGCTG GTGTGGGAGC AGAATGTCTG CAACATCATC 
ATGCTGACAG TATGCATGGA GAACGGGCGG GTTCTCTGTG ATCACTACTG GCCATCCGAA 
TCTGCCCCAG TCTCCTATGG GCACATCCGT GTCCACTTGC TGTCACAGAG CAGCTCCGAG 
GAGTGGACGA TGCGCGAGTT CAAACTGTGG CATGAGGGTA TCCGGGCAGA GCGGCATGTG 
TCCCACCTGC ACTACACGGC ATGGCCTGAC CATGGGATCC CAGAGTCCAC GACTTCCATT 
ATGACCTTCA GAGAGCTGGT CCGGGAGCGT ATCCAGAGCA CCAAGGACGC GGGGCCAACC 
CTCGTGCACT GCAGCGCTGG GGTGGGCCGC ACTGGCACCT TCATTGCACT GGACCGGCTG 
CTGCAGCAGA TGAAGCAGGA GAAGGTGGTG GACACGTTTG GTGTTGTTTA CACCTTGCGG 
ATGAACCGTT ACCTGATGAT TCAGACGCTG TCCCAGTATA TTTTCCTGCA CAGCTGTATT 
CTGGAAAAGA TCTTAGAGGA ACCACTCCTG GGTTTATCAG GGACAGAGAG GTCCTGCCCC 
ATCCCGCTGA AAAGCTTTGC TCAGCACTAT GCCCAGAAGG CAACCAAGTC GCATGTGGGC 
TTCCTGAGGG AGTACGAGAC CCTCCTAGAG GCTGTCAAGG AAGAAGCCAG CTCTGCAACA 
CCATCTTCAG GCGACCAGCA GACACATCCC TCTTCCAGTA TCCTCCCATA TGATCGCTCC 
AGAGTGAAGT TTTCCCTGCT GGAACAGGGC CCATTCTCAG GTCTCCTGCA GGTGTGGCGT 
GTCCCGGGCT GCAGCTCCAG CAGGGAATAT CTGGCTGTCC AGGGGCCTGA CAAGTTGACT 
GTGGAGGACT TCTGGACCCT GGTGTGGGAA CAGGACGTTC ACACCATCCT AACACTTCTC 
CCATGGCAGG AGAAGGGGGA GATCCCAAGT GGGGCGTGCT GGCCCCTGGA AGGAGACTCT 
CTCTGCACAA AGACGCTGAC CATCCAGTGT GGCACAGAGA AGCTTGTCTC GGGATGGAGG 
TGCATCCAGC TCAAAATGAA ACATGAGAAG AAAGCAAAGG AGAGGCAGGT ACAACAGTTC 
CTATACACAC TGTGGAGCAG CAAGAAACAG CCAGATGTCC AAAGCCTGGT GGAGTTCCTC 
ACTGCTGTCA GACGGTGCAT GCCCCACCGA AAGAGAGCCG GTCCTCTCCT GCTGCACTGC 
AGCAGTGGCG TGAGCCAAAT GGGGACACTG ATCTCCCTGG ACTGCCTGTT GCACCAGATG 
AAAGCTGAGA GAACGGTGGA TGTCTATGGG GTCACCCTCC AGCTGGCAAG AAGCTGTTGT 
CTCATGACCC CAACACTGGA CCAGTACATG TTCCTGTACA CCTGCATCCG GGACATCATT 
GCTCAGAAAC AGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106890484 ( XM_014946348.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801834.1 Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X6, mRNA.

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CloneID	OCa68224
Clone ID Related Accession (Same CDS sequence)	XM_014946348.1
Accession Version	XM_014946348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	receptor-type tyrosine-protein phosphatase V-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGG CGTCCTGGGC GCATGCCCAC GGGCAGCGAG ACAGCTACCA CCTAGCCCTC 
TACCACAGCG ACTCCCAGAC GCTGGTGAGA AATGTGTCCA TCCTGCCAAA CGCCTCTACC 
TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT GAGTACGCCT TGAAGGTCAG CACGCTGGTT 
GGATCCAGTC AGGCGAGCAC CAGTATCCAC CAGTGGACAG CTCCCTCCAT CCCCACCCAG 
CTGAGACTCA GCCCAGGATC CAGCACCAGC CTCATCGCCT CCTGGGCAGA TGCTGGGGGG 
GCTGCCTGGC TGCACCTCAC ACTCCACAAC CTCCTCACCC AGATGGTGAC CACCACCCTG 
TCAGCCAGAA GGGGCCTCAC CAGCTACACC TTCCAGCATC TCCACTCCGG CACCCAGTAC 
TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC TACACCATGG TGGGGCCCAA TGCCACCGCT 
TGGACATACC CCCTGAGCCC TGGAAATGTG ACCCTGAGCC CTGCAGAGGA ACAGAACTCC 
TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA GAGAGGGACT TCTACCTGGT GACACTGCAG 
GAGGAAGAGG ATGGTGTGCC AACAAGGAAC ATCTCTGTCG ATGGAGACAA CAGTCATGTC 
ACTTTCCACG GGCTGGATCC TGGGAAGCAA TACTCTGTCC AGGTGACAGC AGTGGCTGGG 
CCTTACAGAG CATCAGCCCA CAGCACAGCA GCCTGGACAC AGCCGCTAGC ACCATCTGGT 
GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC AGCCTACTGG CCAGCTGGGA GGAGGCAATG 
GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC ATGGAGCACC CTGTGAAGAA TAGCTCGCTG 
GTTAAAGGTG TCACCAACTT CACCTACAAC GGCCTCCACC CTGGGACTCT TTACACCTTT 
GAGGTCAGCA CCGTGGCTGG CCCCTACACA TCCTCGCCCC GGCGCATCAC CAACTGGACA 
TATCCTTTGC CCCTGGAGCA GCTGACCCTC AGCAATCAGG GCCACAGCAC CTCTCTGCAA 
GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC ACTGGCTACG CAGGCACCCT CTGGGAAACC 
AAGTCCCAGG AGCGGGTCAG GAACATGACT GTGGGGAACA ACTGGATGAA TGCCACCTTT 
GAGGACCTGG TCCCTGGGCG GCAGTATACA CTGGAGATGA CTGCTATGGC CGGACCTTAC 
AGATCCCCTG TGCGATCAGC CACAGACTGG ACATACCCTC TGGCTCCAGC CGGCGTGACC 
CTGACCAACA CCCGACGCCC ACTGGGACTT GCTGCCTTCT GGGACAAGGC TGCTGGTGAT 
GTGGACCAGT TCCACCTCCA GCTCTACAGC AAGAGCCATC CAGCACAGAG GAACATCTCA 
GTAGGGCCAG ACACCCACAA CTTCACATTC CTAGGGCTGT CCCCTGGGAT TCAGTACTTC 
CTGAAGGTGA CTGTCCTCGC TGGCCCATAC AGATCCTCGT CACACTTTGC CACTGAGTGG 
ACATATCCAC TGTCTCTGGC TAACGTGAGT GTACAGCCTG GCCGGAGACC TCAGGAGCTA 
CATGTGAGCT GGGTGGAGTC AGGTGGTGGC AGAGACCACT TGGTACAGCT CTCAGTGGCC 
GAGTCCCTAT CCATCATAAA GAATGTGTCT GTCCCCCGTG GAGTCAACCA GCTTGACCTG 
GAGGGGCTGG TTCCAGGGTC TCGATACCGT GTGGAGATCA TTTCTCAGGC TGGGCCTCAT 
CGCACCTCCT CTCAGACTGC CATTGGCTAC ACTGTCCCAC TACCTCCGCT TTCCCTCTCG 
GCAAGCCCTG TCAGCACTGC CTGGGCTCTG GCTGTGCACT GGGAGACCCC TTCTGGGCAG 
AGGGATGGAT ATCTGCTCAG TGTGCATGAG GAAGGTTCTT CTAAACCAGC AAGGAACCTG 
AAAGTAGGGA AAGACAGCAC CAATGTCACC CTGGCACAGC TGGAACCAGG AACTTGCTAC 
CTTGTTGAGA TCTGGGCTGT AGCTGGACCT TACCGCTCAC TCCCCAAGAA CTTCACTGGC 
TGCACAGTTC CTGCTGCACC GACAGACCTG AGCCTCACCA ACCCAGGCAG CTCCTCAGAG 
CTTTACACAT GCTGGAACAA GCCCCCCGGC AGGAGGGATC GCTACCATGT TATCCTGTAT 
GGCCTCAGCA TCCAGAGTAG GAATCGGGTC CAGACCTTGA GTCCAGATGC CCAGAACATC 
ACCTGGACTC ACCTGGAGGC AGGCAGCAGG TTTGCTGTGC AGGTCACTGC TGTGAAAGGT 
TCATTAGAAG CCTCCTCCAC CAACGTCACC CAATGGACAT ATCCCTTGGC CCCTGCCAAC 
CTCACCCTGG GCAGCCCTTC TGCCTCCGAG CTGCAGGCCT CCTGGGCAAC AACGGGGCGA 
GGAGCAGAAG GCTATGTGGT GGATGTCTAT GATAGAGCCT CCAGCAGTCA CGTTGGGCAC 
GTGGTGCTGG GTGGGGATGC CCGGAGTCAC ACCTTCAGAA AGCTGAGCCC TGGTACTCGC 
TACAGCGTGG CAGTGAGGGC CACAGCTGGG CCCTTCCACA CCAGCACCCC CAACTTCACC 
CTCTGCACAC GCCCGCTGCC CCCAGCCGCC GTGCGCTTGC TGAGCACGGG GCACCCCGAC 
AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT GGGGGGCGAG ACGGCTACAC ACTGACCCTG 
TACCACGGGC GGCTGGGCAG CATGGTGGCT ACCACCTCTC TTGGGAGAGA CACCCACAAC 
TTCACCTTCA TGGGTCTGAA CCCAGGATAC AAGTACTCCC TGGAGGCCAG TGCCATGGCT 
GGACCATACC ACGCAGCAGC ACCCAACATC AGTGCCTGGA CACGCCCGCT GCCCCCAGCC 
TCCGTGCGCT TGCTGAGCAC GGGGCACCCC GACAGGCTGA GTGCGTCCTG GGCAGCCGCG 
GCTGGGGGGC GAGACGGCTA CACACTGACC CTGTACCACG GGCGGCTGGG CAGCATGGTG 
GCTACCACCT CTCTTGGGAG AGACACCCAC AACTTCACCT TCACGGGCCT GAACCCAGGA 
AGCAAGTATG TTCTGGAGGC GGGATCCATA GCAGGGTCCT ACCAGACACC TGCAGGGAAC 
ATCAGCAACT GGACGTACCC CCTGGCACCT CGTAACGTGT ACATGACGAA CCAGGGGTAT 
CCCAACAGGC TGAGTGTGTC CTGGCGAGCT GAACCCCAAG GGCAAGACAG TTACAGGCTC 
CTCCTGTATC ATTCGGGATC AGGTATTCTG ACAGCCAATG TATCTGTTGA GAAAGGCACC 
AGCAAATTCA CCTTTTCTGG CCTGGCTCCG GGACATAAAT ACCTGCTGGA AGTGGTGTCC 
ACGGCAGGGC CCTACGCAGC CTCTGCAGGG AACATCAGTG ACTGGACAAC CCCCTCAGTT 
CCCAAAAATC TCTCTGCGAT GGCTGAAGGG AACAACACAG TGCTCATCTC CTGGGACAGT 
GTCTCTGGAC AACAGGATGA GTGCCAGTTG TGGCTGCGGG ATCCCAGGAA CAGCACACTG 
CCCTGGCGGC ACGCTCTCAG CCAAGGGCAA GTCCACCACC TCCTCCAGGG GCTGGTCCCA 
GGGAGGAACT ACTCTGTCTC CTTGAGCTGC GTGGCCGGGC CCTACTGGAG CAGCACCAAA 
CCCTTGGCGG TGCTGATGGA GCCAAACCCA GTGGAAGATG TGCAATGCTT ATCAGAGTCA 
AGGAGCCTTT ACTTGAACTG GACCAGCTCT CCTGGAGATG TGGAGGCTTA TGAAGTGGTG 
ACAGAGAGAC TTTCTGATGG ACTTCCCACC TCCAAGTATG TCATGAGCAT CCCTATGAGT 
GAGGCCAGCC TGGAGGGGCT GCAGCCAAAC TCGTCATACC GAATCGCTGT GAGCACAGTG 
GGCATGAACA CAATTAGGAG CCAGGCTGTG ACTCTGCTCT GCAACACAAC AGTGGAGGCC 
TTGCCTCCGC CCCTGCGAGC AGACATCTTC CAAGTAGAGG CCAGCTCCGC AGTTATCATT 
TCATCCGACC TGTTCAGTGA AGAGAATGGC CAGATTGAGT ATTACGGTGT CGTTGCTACC 
ACTAATGATT CACTGCTGAG GCCCACCCAG GAAATCATGT CCAGCACATG GTATGACCAC 
TATTACGGGA CAGAGGACTC CTACCTGGCT GTGCTAATCC CCAATCCTTT CCATTCGAGC 
CCCAGGAGCT CCCCCGAAAC CTGGCGAGTG CCAGTGGGAA TAGAGGAGTG CGGCCAGTCC 
AGGGCAATGT GCAACGGGAA GCTGAAAGCC AACGAGCAGT ACAGGTTCAG CATTGCTGCC 
TTCACCAAAT ACGACCCAGT AGCCCCTGCA GTGACCTTCA CCATGTTCTC AGCTGCTGAA 
TCCAGTGCAG ATGCAACTCC ACTCTCAATG CCAATAATCA CTGGAATCAT CGTGGGATTT 
CTCTTGACAC TTGCAGCTAT TTTTGCTTTG GTCTACTGGA AACAGCTCAG AGCAAAGAGA 
ACCAACAAAA GCAGCCTGCC CCAGGAAATG GTGACCTACA GCTTGAGAAA CATCCATCGG 
CCAATTCCCA TACAGAACTT CAAGCAGTAC TATGAGTTGA AGACAGCAAG TGCTAACCAC 
GCTTTTTTCC AGGAGTTTGA GGAGCTGAAG GAAGTTGGGA AGGAGCAGCC AAAGGTGGAG 
GCTGAGCTAC CAGCAAATGT CTCCAAGAAC AGATATCCCC ACGTGCTGCC CTATGATCAC 
TCTCGGGTCA AGCTGAGCCA ACTGGGGGAG GATCCACACT CTGACTACAT CAATGCCAAC 
TTCATGCCTG GCTATACATC CCAGCAGGAG TTCATTGCCA CCCAGGGGCC CCTGAAGAAA 
ACGATAGAGG ACTTCTGGAG GCTGGTGTGG GAGCAGAATG TCTGCAACAT CATCATGCTG 
ACAGTATGCA TGGAGAACGG GCGGGTTCTC TGTGATCACT ACTGGCCATC CGAATCTGCC 
CCAGTCTCCT ATGGGCACAT CCGTGTCCAC TTGCTGTCAC AGAGCAGCTC CGAGGAGTGG 
ACGATGCGCG AGTTCAAACT GTGGCATGAG GGTATCCGGG CAGAGCGGCA TGTGTCCCAC 
CTGCACTACA CGGCATGGCC TGACCATGGG ATCCCAGAGT CCACGACTTC CATTATGACC 
TTCAGAGAGC TGGTCCGGGA GCGTATCCAG AGCACCAAGG ACGCGGGGCC AACCCTCGTG 
CACTGCAGCG CTGGGGTGGG CCGCACTGGC ACCTTCATTG CACTGGACCG GCTGCTGCAG 
CAGATGAAGC AGGAGAAGGT GGTGGACACG TTTGGTGTTG TTTACACCTT GCGGATGAAC 
CGTTACCTGA TGATTCAGAC GCTGTCCCAG TATATTTTCC TGCACAGCTG TATTCTGGAA 
AAGATCTTAG AGGAACCACT CCTGGGTTTA TCAGGGACAG AGAGGTCCTG CCCCATCCCG 
CTGAAAAGCT TTGCTCAGCA CTATGCCCAG AAGGCAACCA AGTCGCATGT GGGCTTCCTG 
AGGGAGTACG AGACCCTCCT AGAGGCTGTC AAGGAAGAAG CCAGCTCTGC AACACCATCT 
TCAGGCGACC AGCAGACACA TCCCTCTTCC AGTATCCTCC CATATGATCG CTCCAGAGTG 
AAGTTTTCCC TGCTGGAACA GGGCCCATTC TCAGGTCTCC TGCAGGTGTG GCGTGTCCCG 
GGCTGCAGCT CCAGCAGGGA ATATCTGGCT GTCCAGGGGC CTGACAAGTT GACTGTGGAG 
GACTTCTGGA CCCTGGTGTG GGAACAGGAC GTTCACACCA TCCTAACACT TCTCCCATGG 
CAGGAGAAGG GGGAGATCCC AAGTGGGGCG TGCTGGCCCC TGGAAGGAGA CTCTCTCTGC 
ACAAAGACGC TGACCATCCA GTGTGGCACA GAGAAGCTTG TCTCGGGATG GAGGTGCATC 
CAGCTCAAAA TGAAACATGA GAAGAAAGCA AAGGAGAGGC AGGTACAACA GTTCCTATAC 
ACACTGTGGA GCAGCAAGAA ACAGCCAGAT GTCCAAAGCC TGGTGGAGTT CCTCACTGCT 
GTCAGACGGT GCATGCCCCA CCGAAAGAGA GCCGGTCCTC TCCTGCTGCA CTGCAGCAGT 
GGCGTGAGCC AAATGGGGAC ACTGATCTCC CTGGACTGCC TGTTGCACCA GATGAAAGCT 
GAGAGAACGG TGGATGTCTA TGGGGTCACC CTCCAGCTGG CAAGAAGCTG TTGTCTCATG 
ACCCCAACAC TGGACCAGTA CATGTTCCTG TACACCTGCA TCCGGGACAT CATTGCTCAG 
AAACAGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801834.1
CDS	256..6627
Translation	MLQASWAHAHGQRDSYHLALYHSDSQTLVRNVSILPNASTFLFDGLLPGSEYALKVSTLVGSSQASTSIHQWTAPSIPTQLRLSPGSSTSLIASWADAGGAAWLHLTLHNLLTQMVTTTLSARRGLTSYTFQHLHSGTQYWLGLSAMAGPYTMVGPNATAWTYPLSPGNVTLSPAEEQNSLQARWSTPAGERDFYLVTLQEEEDGVPTRNISVDGDNSHVTFHGLDPGKQYSVQVTAVAGPYRASAHSTAAWTQPLAPSGVSLSSQGSPYSLLASWEEAMGEGYVLALSPMEHPVKNSSLVKGVTNFTYNGLHPGTLYTFEVSTVAGPYTSSPRRITNWTYPLPLEQLTLSNQGHSTSLQASWRAAPAGSTGYAGTLWETKSQERVRNMTVGNNWMNATFEDLVPGRQYTLEMTAMAGPYRSPVRSATDWTYPLAPAGVTLTNTRRPLGLAAFWDKAAGDVDQFHLQLYSKSHPAQRNISVGPDTHNFTFLGLSPGIQYFLKVTVLAGPYRSSSHFATEWTYPLSLANVSVQPGRRPQELHVSWVESGGGRDHLVQLSVAESLSIIKNVSVPRGVNQLDLEGLVPGSRYRVEIISQAGPHRTSSQTAIGYTVPLPPLSLSASPVSTAWALAVHWETPSGQRDGYLLSVHEEGSSKPARNLKVGKDSTNVTLAQLEPGTCYLVEIWAVAGPYRSLPKNFTGCTVPAAPTDLSLTNPGSSSELYTCWNKPPGRRDRYHVILYGLSIQSRNRVQTLSPDAQNITWTHLEAGSRFAVQVTAVKGSLEASSTNVTQWTYPLAPANLTLGSPSASELQASWATTGRGAEGYVVDVYDRASSSHVGHVVLGGDARSHTFRKLSPGTRYSVAVRATAGPFHTSTPNFTLCTRPLPPAAVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFMGLNPGYKYSLEASAMAGPYHAAAPNISAWTRPLPPASVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFTGLNPGSKYVLEAGSIAGSYQTPAGNISNWTYPLAPRNVYMTNQGYPNRLSVSWRAEPQGQDSYRLLLYHSGSGILTANVSVEKGTSKFTFSGLAPGHKYLLEVVSTAGPYAASAGNISDWTTPSVPKNLSAMAEGNNTVLISWDSVSGQQDECQLWLRDPRNSTLPWRHALSQGQVHHLLQGLVPGRNYSVSLSCVAGPYWSSTKPLAVLMEPNPVEDVQCLSESRSLYLNWTSSPGDVEAYEVVTERLSDGLPTSKYVMSIPMSEASLEGLQPNSSYRIAVSTVGMNTIRSQAVTLLCNTTVEALPPPLRADIFQVEASSAVIISSDLFSEENGQIEYYGVVATTNDSLLRPTQEIMSSTWYDHYYGTEDSYLAVLIPNPFHSSPRSSPETWRVPVGIEECGQSRAMCNGKLKANEQYRFSIAAFTKYDPVAPAVTFTMFSAAESSADATPLSMPIITGIIVGFLLTLAAIFALVYWKQLRAKRTNKSSLPQEMVTYSLRNIHRPIPIQNFKQYYELKTASANHAFFQEFEELKEVGKEQPKVEAELPANVSKNRYPHVLPYDHSRVKLSQLGEDPHSDYINANFMPGYTSQQEFIATQGPLKKTIEDFWRLVWEQNVCNIIMLTVCMENGRVLCDHYWPSESAPVSYGHIRVHLLSQSSSEEWTMREFKLWHEGIRAERHVSHLHYTAWPDHGIPESTTSIMTFRELVRERIQSTKDAGPTLVHCSAGVGRTGTFIALDRLLQQMKQEKVVDTFGVVYTLRMNRYLMIQTLSQYIFLHSCILEKILEEPLLGLSGTERSCPIPLKSFAQHYAQKATKSHVGFLREYETLLEAVKEEASSATPSSGDQQTHPSSSILPYDRSRVKFSLLEQGPFSGLLQVWRVPGCSSSREYLAVQGPDKLTVEDFWTLVWEQDVHTILTLLPWQEKGEIPSGACWPLEGDSLCTKTLTIQCGTEKLVSGWRCIQLKMKHEKKAKERQVQQFLYTLWSSKKQPDVQSLVEFLTAVRRCMPHRKRAGPLLLHCSSGVSQMGTLISLDCLLHQMKAERTVDVYGVTLQLARSCCLMTPTLDQYMFLYTCIRDIIAQKQP

Target ORF information:

RefSeq Version	XM_014946348.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946348.1

ORF Insert Sequence:

ATGCTGCAGG CGTCCTGGGC GCATGCCCAC GGGCAGCGAG ACAGCTACCA CCTAGCCCTC 
TACCACAGCG ACTCCCAGAC GCTGGTGAGA AATGTGTCCA TCCTGCCAAA CGCCTCTACC 
TTCCTGTTTG ACGGGTTGCT ACCTGGCAGT GAGTACGCCT TGAAGGTCAG CACGCTGGTT 
GGATCCAGTC AGGCGAGCAC CAGTATCCAC CAGTGGACAG CTCCCTCCAT CCCCACCCAG 
CTGAGACTCA GCCCAGGATC CAGCACCAGC CTCATCGCCT CCTGGGCAGA TGCTGGGGGG 
GCTGCCTGGC TGCACCTCAC ACTCCACAAC CTCCTCACCC AGATGGTGAC CACCACCCTG 
TCAGCCAGAA GGGGCCTCAC CAGCTACACC TTCCAGCATC TCCACTCCGG CACCCAGTAC 
TGGTTGGGGC TGAGTGCCAT GGCTGGGCCC TACACCATGG TGGGGCCCAA TGCCACCGCT 
TGGACATACC CCCTGAGCCC TGGAAATGTG ACCCTGAGCC CTGCAGAGGA ACAGAACTCC 
TTGCAGGCTC GCTGGAGCAC CCCAGCGGGA GAGAGGGACT TCTACCTGGT GACACTGCAG 
GAGGAAGAGG ATGGTGTGCC AACAAGGAAC ATCTCTGTCG ATGGAGACAA CAGTCATGTC 
ACTTTCCACG GGCTGGATCC TGGGAAGCAA TACTCTGTCC AGGTGACAGC AGTGGCTGGG 
CCTTACAGAG CATCAGCCCA CAGCACAGCA GCCTGGACAC AGCCGCTAGC ACCATCTGGT 
GTGAGTCTGT CCAGCCAAGG GAGCCCCTAC AGCCTACTGG CCAGCTGGGA GGAGGCAATG 
GGAGAGGGCT ACGTGCTGGC CCTCAGCCCC ATGGAGCACC CTGTGAAGAA TAGCTCGCTG 
GTTAAAGGTG TCACCAACTT CACCTACAAC GGCCTCCACC CTGGGACTCT TTACACCTTT 
GAGGTCAGCA CCGTGGCTGG CCCCTACACA TCCTCGCCCC GGCGCATCAC CAACTGGACA 
TATCCTTTGC CCCTGGAGCA GCTGACCCTC AGCAATCAGG GCCACAGCAC CTCTCTGCAA 
GCCTCCTGGA GAGCAGCTCC CGCTGGCAGC ACTGGCTACG CAGGCACCCT CTGGGAAACC 
AAGTCCCAGG AGCGGGTCAG GAACATGACT GTGGGGAACA ACTGGATGAA TGCCACCTTT 
GAGGACCTGG TCCCTGGGCG GCAGTATACA CTGGAGATGA CTGCTATGGC CGGACCTTAC 
AGATCCCCTG TGCGATCAGC CACAGACTGG ACATACCCTC TGGCTCCAGC CGGCGTGACC 
CTGACCAACA CCCGACGCCC ACTGGGACTT GCTGCCTTCT GGGACAAGGC TGCTGGTGAT 
GTGGACCAGT TCCACCTCCA GCTCTACAGC AAGAGCCATC CAGCACAGAG GAACATCTCA 
GTAGGGCCAG ACACCCACAA CTTCACATTC CTAGGGCTGT CCCCTGGGAT TCAGTACTTC 
CTGAAGGTGA CTGTCCTCGC TGGCCCATAC AGATCCTCGT CACACTTTGC CACTGAGTGG 
ACATATCCAC TGTCTCTGGC TAACGTGAGT GTACAGCCTG GCCGGAGACC TCAGGAGCTA 
CATGTGAGCT GGGTGGAGTC AGGTGGTGGC AGAGACCACT TGGTACAGCT CTCAGTGGCC 
GAGTCCCTAT CCATCATAAA GAATGTGTCT GTCCCCCGTG GAGTCAACCA GCTTGACCTG 
GAGGGGCTGG TTCCAGGGTC TCGATACCGT GTGGAGATCA TTTCTCAGGC TGGGCCTCAT 
CGCACCTCCT CTCAGACTGC CATTGGCTAC ACTGTCCCAC TACCTCCGCT TTCCCTCTCG 
GCAAGCCCTG TCAGCACTGC CTGGGCTCTG GCTGTGCACT GGGAGACCCC TTCTGGGCAG 
AGGGATGGAT ATCTGCTCAG TGTGCATGAG GAAGGTTCTT CTAAACCAGC AAGGAACCTG 
AAAGTAGGGA AAGACAGCAC CAATGTCACC CTGGCACAGC TGGAACCAGG AACTTGCTAC 
CTTGTTGAGA TCTGGGCTGT AGCTGGACCT TACCGCTCAC TCCCCAAGAA CTTCACTGGC 
TGCACAGTTC CTGCTGCACC GACAGACCTG AGCCTCACCA ACCCAGGCAG CTCCTCAGAG 
CTTTACACAT GCTGGAACAA GCCCCCCGGC AGGAGGGATC GCTACCATGT TATCCTGTAT 
GGCCTCAGCA TCCAGAGTAG GAATCGGGTC CAGACCTTGA GTCCAGATGC CCAGAACATC 
ACCTGGACTC ACCTGGAGGC AGGCAGCAGG TTTGCTGTGC AGGTCACTGC TGTGAAAGGT 
TCATTAGAAG CCTCCTCCAC CAACGTCACC CAATGGACAT ATCCCTTGGC CCCTGCCAAC 
CTCACCCTGG GCAGCCCTTC TGCCTCCGAG CTGCAGGCCT CCTGGGCAAC AACGGGGCGA 
GGAGCAGAAG GCTATGTGGT GGATGTCTAT GATAGAGCCT CCAGCAGTCA CGTTGGGCAC 
GTGGTGCTGG GTGGGGATGC CCGGAGTCAC ACCTTCAGAA AGCTGAGCCC TGGTACTCGC 
TACAGCGTGG CAGTGAGGGC CACAGCTGGG CCCTTCCACA CCAGCACCCC CAACTTCACC 
CTCTGCACAC GCCCGCTGCC CCCAGCCGCC GTGCGCTTGC TGAGCACGGG GCACCCCGAC 
AGGCTGAGTG CGTCCTGGGC AGCCGCGGCT GGGGGGCGAG ACGGCTACAC ACTGACCCTG 
TACCACGGGC GGCTGGGCAG CATGGTGGCT ACCACCTCTC TTGGGAGAGA CACCCACAAC 
TTCACCTTCA TGGGTCTGAA CCCAGGATAC AAGTACTCCC TGGAGGCCAG TGCCATGGCT 
GGACCATACC ACGCAGCAGC ACCCAACATC AGTGCCTGGA CACGCCCGCT GCCCCCAGCC 
TCCGTGCGCT TGCTGAGCAC GGGGCACCCC GACAGGCTGA GTGCGTCCTG GGCAGCCGCG 
GCTGGGGGGC GAGACGGCTA CACACTGACC CTGTACCACG GGCGGCTGGG CAGCATGGTG 
GCTACCACCT CTCTTGGGAG AGACACCCAC AACTTCACCT TCACGGGCCT GAACCCAGGA 
AGCAAGTATG TTCTGGAGGC GGGATCCATA GCAGGGTCCT ACCAGACACC TGCAGGGAAC 
ATCAGCAACT GGACGTACCC CCTGGCACCT CGTAACGTGT ACATGACGAA CCAGGGGTAT 
CCCAACAGGC TGAGTGTGTC CTGGCGAGCT GAACCCCAAG GGCAAGACAG TTACAGGCTC 
CTCCTGTATC ATTCGGGATC AGGTATTCTG ACAGCCAATG TATCTGTTGA GAAAGGCACC 
AGCAAATTCA CCTTTTCTGG CCTGGCTCCG GGACATAAAT ACCTGCTGGA AGTGGTGTCC 
ACGGCAGGGC CCTACGCAGC CTCTGCAGGG AACATCAGTG ACTGGACAAC CCCCTCAGTT 
CCCAAAAATC TCTCTGCGAT GGCTGAAGGG AACAACACAG TGCTCATCTC CTGGGACAGT 
GTCTCTGGAC AACAGGATGA GTGCCAGTTG TGGCTGCGGG ATCCCAGGAA CAGCACACTG 
CCCTGGCGGC ACGCTCTCAG CCAAGGGCAA GTCCACCACC TCCTCCAGGG GCTGGTCCCA 
GGGAGGAACT ACTCTGTCTC CTTGAGCTGC GTGGCCGGGC CCTACTGGAG CAGCACCAAA 
CCCTTGGCGG TGCTGATGGA GCCAAACCCA GTGGAAGATG TGCAATGCTT ATCAGAGTCA 
AGGAGCCTTT ACTTGAACTG GACCAGCTCT CCTGGAGATG TGGAGGCTTA TGAAGTGGTG 
ACAGAGAGAC TTTCTGATGG ACTTCCCACC TCCAAGTATG TCATGAGCAT CCCTATGAGT 
GAGGCCAGCC TGGAGGGGCT GCAGCCAAAC TCGTCATACC GAATCGCTGT GAGCACAGTG 
GGCATGAACA CAATTAGGAG CCAGGCTGTG ACTCTGCTCT GCAACACAAC AGTGGAGGCC 
TTGCCTCCGC CCCTGCGAGC AGACATCTTC CAAGTAGAGG CCAGCTCCGC AGTTATCATT 
TCATCCGACC TGTTCAGTGA AGAGAATGGC CAGATTGAGT ATTACGGTGT CGTTGCTACC 
ACTAATGATT CACTGCTGAG GCCCACCCAG GAAATCATGT CCAGCACATG GTATGACCAC 
TATTACGGGA CAGAGGACTC CTACCTGGCT GTGCTAATCC CCAATCCTTT CCATTCGAGC 
CCCAGGAGCT CCCCCGAAAC CTGGCGAGTG CCAGTGGGAA TAGAGGAGTG CGGCCAGTCC 
AGGGCAATGT GCAACGGGAA GCTGAAAGCC AACGAGCAGT ACAGGTTCAG CATTGCTGCC 
TTCACCAAAT ACGACCCAGT AGCCCCTGCA GTGACCTTCA CCATGTTCTC AGCTGCTGAA 
TCCAGTGCAG ATGCAACTCC ACTCTCAATG CCAATAATCA CTGGAATCAT CGTGGGATTT 
CTCTTGACAC TTGCAGCTAT TTTTGCTTTG GTCTACTGGA AACAGCTCAG AGCAAAGAGA 
ACCAACAAAA GCAGCCTGCC CCAGGAAATG GTGACCTACA GCTTGAGAAA CATCCATCGG 
CCAATTCCCA TACAGAACTT CAAGCAGTAC TATGAGTTGA AGACAGCAAG TGCTAACCAC 
GCTTTTTTCC AGGAGTTTGA GGAGCTGAAG GAAGTTGGGA AGGAGCAGCC AAAGGTGGAG 
GCTGAGCTAC CAGCAAATGT CTCCAAGAAC AGATATCCCC ACGTGCTGCC CTATGATCAC 
TCTCGGGTCA AGCTGAGCCA ACTGGGGGAG GATCCACACT CTGACTACAT CAATGCCAAC 
TTCATGCCTG GCTATACATC CCAGCAGGAG TTCATTGCCA CCCAGGGGCC CCTGAAGAAA 
ACGATAGAGG ACTTCTGGAG GCTGGTGTGG GAGCAGAATG TCTGCAACAT CATCATGCTG 
ACAGTATGCA TGGAGAACGG GCGGGTTCTC TGTGATCACT ACTGGCCATC CGAATCTGCC 
CCAGTCTCCT ATGGGCACAT CCGTGTCCAC TTGCTGTCAC AGAGCAGCTC CGAGGAGTGG 
ACGATGCGCG AGTTCAAACT GTGGCATGAG GGTATCCGGG CAGAGCGGCA TGTGTCCCAC 
CTGCACTACA CGGCATGGCC TGACCATGGG ATCCCAGAGT CCACGACTTC CATTATGACC 
TTCAGAGAGC TGGTCCGGGA GCGTATCCAG AGCACCAAGG ACGCGGGGCC AACCCTCGTG 
CACTGCAGCG CTGGGGTGGG CCGCACTGGC ACCTTCATTG CACTGGACCG GCTGCTGCAG 
CAGATGAAGC AGGAGAAGGT GGTGGACACG TTTGGTGTTG TTTACACCTT GCGGATGAAC 
CGTTACCTGA TGATTCAGAC GCTGTCCCAG TATATTTTCC TGCACAGCTG TATTCTGGAA 
AAGATCTTAG AGGAACCACT CCTGGGTTTA TCAGGGACAG AGAGGTCCTG CCCCATCCCG 
CTGAAAAGCT TTGCTCAGCA CTATGCCCAG AAGGCAACCA AGTCGCATGT GGGCTTCCTG 
AGGGAGTACG AGACCCTCCT AGAGGCTGTC AAGGAAGAAG CCAGCTCTGC AACACCATCT 
TCAGGCGACC AGCAGACACA TCCCTCTTCC AGTATCCTCC CATATGATCG CTCCAGAGTG 
AAGTTTTCCC TGCTGGAACA GGGCCCATTC TCAGGTCTCC TGCAGGTGTG GCGTGTCCCG 
GGCTGCAGCT CCAGCAGGGA ATATCTGGCT GTCCAGGGGC CTGACAAGTT GACTGTGGAG 
GACTTCTGGA CCCTGGTGTG GGAACAGGAC GTTCACACCA TCCTAACACT TCTCCCATGG 
CAGGAGAAGG GGGAGATCCC AAGTGGGGCG TGCTGGCCCC TGGAAGGAGA CTCTCTCTGC 
ACAAAGACGC TGACCATCCA GTGTGGCACA GAGAAGCTTG TCTCGGGATG GAGGTGCATC 
CAGCTCAAAA TGAAACATGA GAAGAAAGCA AAGGAGAGGC AGGTACAACA GTTCCTATAC 
ACACTGTGGA GCAGCAAGAA ACAGCCAGAT GTCCAAAGCC TGGTGGAGTT CCTCACTGCT 
GTCAGACGGT GCATGCCCCA CCGAAAGAGA GCCGGTCCTC TCCTGCTGCA CTGCAGCAGT 
GGCGTGAGCC AAATGGGGAC ACTGATCTCC CTGGACTGCC TGTTGCACCA GATGAAAGCT 
GAGAGAACGG TGGATGTCTA TGGGGTCACC CTCCAGCTGG CAAGAAGCTG TTGTCTCATG 
ACCCCAACAC TGGACCAGTA CATGTTCCTG TACACCTGCA TCCGGGACAT CATTGCTCAG 
AAACAGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106890484 ( XM_014946349.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014801835.1 Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X7, mRNA.

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CloneID	OCa68225
Clone ID Related Accession (Same CDS sequence)	XM_014946349.1
Accession Version	XM_014946349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	receptor-type tyrosine-protein phosphatase V-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGG GCTACGTGCT GGCCCTCAGC CCCATGGAGC ACCCTGTGAA GAATAGCTCG 
CTGGTTAAAG GTGTCACCAA CTTCACCTAC AACGGCCTCC ACCCTGGGAC TCTTTACACC 
TTTGAGGTCA GCACCGTGGC TGGCCCCTAC ACATCCTCGC CCCGGCGCAT CACCAACTGG 
ACATATCCTT TGCCCCTGGA GCAGCTGACC CTCAGCAATC AGGGCCACAG CACCTCTCTG 
CAAGCCTCCT GGAGAGCAGC TCCCGCTGGC AGCACTGGCT ACGCAGGCAC CCTCTGGGAA 
ACCAAGTCCC AGGAGCGGGT CAGGAACATG ACTGTGGGGA ACAACTGGAT GAATGCCACC 
TTTGAGGACC TGGTCCCTGG GCGGCAGTAT ACACTGGAGA TGACTGCTAT GGCCGGACCT 
TACAGATCCC CTGTGCGATC AGCCACAGAC TGGACATACC CTCTGGCTCC AGCCGGCGTG 
ACCCTGACCA ACACCCGACG CCCACTGGGA CTTGCTGCCT TCTGGGACAA GGCTGCTGGT 
GATGTGGACC AGTTCCACCT CCAGCTCTAC AGCAAGAGCC ATCCAGCACA GAGGAACATC 
TCAGTAGGGC CAGACACCCA CAACTTCACA TTCCTAGGGC TGTCCCCTGG GATTCAGTAC 
TTCCTGAAGG TGACTGTCCT CGCTGGCCCA TACAGATCCT CGTCACACTT TGCCACTGAG 
TGGACATATC CACTGTCTCT GGCTAACGTG AGTGTACAGC CTGGCCGGAG ACCTCAGGAG 
CTACATGTGA GCTGGGTGGA GTCAGGTGGT GGCAGAGACC ACTTGGTACA GCTCTCAGTG 
GCCGAGTCCC TATCCATCAT AAAGAATGTG TCTGTCCCCC GTGGAGTCAA CCAGCTTGAC 
CTGGAGGGGC TGGTTCCAGG GTCTCGATAC CGTGTGGAGA TCATTTCTCA GGCTGGGCCT 
CATCGCACCT CCTCTCAGAC TGCCATTGGC TACACTGTCC CACTACCTCC GCTTTCCCTC 
TCGGCAAGCC CTGTCAGCAC TGCCTGGGCT CTGGCTGTGC ACTGGGAGAC CCCTTCTGGG 
CAGAGGGATG GATATCTGCT CAGTGTGCAT GAGGAAGGTT CTTCTAAACC AGCAAGGAAC 
CTGAAAGTAG GGAAAGACAG CACCAATGTC ACCCTGGCAC AGCTGGAACC AGGAACTTGC 
TACCTTGTTG AGATCTGGGC TGTAGCTGGA CCTTACCGCT CACTCCCCAA GAACTTCACT 
GGCTGCACAG TTCCTGCTGC ACCGACAGAC CTGAGCCTCA CCAACCCAGG CAGCTCCTCA 
GAGCTTTACA CATGCTGGAA CAAGCCCCCC GGCAGGAGGG ATCGCTACCA TGTTATCCTG 
TATGGCCTCA GCATCCAGAG TAGGAATCGG GTCCAGACCT TGAGTCCAGA TGCCCAGAAC 
ATCACCTGGA CTCACCTGGA GGCAGGCAGC AGGTTTGCTG TGCAGGTCAC TGCTGTGAAA 
GGTTCATTAG AAGCCTCCTC CACCAACGTC ACCCAATGGA CATATCCCTT GGCCCCTGCC 
AACCTCACCC TGGGCAGCCC TTCTGCCTCC GAGCTGCAGG CCTCCTGGGC AACAACGGGG 
CGAGGAGCAG AAGGCTATGT GGTGGATGTC TATGATAGAG CCTCCAGCAG TCACGTTGGG 
CACGTGGTGC TGGGTGGGGA TGCCCGGAGT CACACCTTCA GAAAGCTGAG CCCTGGTACT 
CGCTACAGCG TGGCAGTGAG GGCCACAGCT GGGCCCTTCC ACACCAGCAC CCCCAACTTC 
ACCCTCTGCA CACGCCCGCT GCCCCCAGCC GCCGTGCGCT TGCTGAGCAC GGGGCACCCC 
GACAGGCTGA GTGCGTCCTG GGCAGCCGCG GCTGGGGGGC GAGACGGCTA CACACTGACC 
CTGTACCACG GGCGGCTGGG CAGCATGGTG GCTACCACCT CTCTTGGGAG AGACACCCAC 
AACTTCACCT TCATGGGTCT GAACCCAGGA TACAAGTACT CCCTGGAGGC CAGTGCCATG 
GCTGGACCAT ACCACGCAGC AGCACCCAAC ATCAGTGCCT GGACACGCCC GCTGCCCCCA 
GCCTCCGTGC GCTTGCTGAG CACGGGGCAC CCCGACAGGC TGAGTGCGTC CTGGGCAGCC 
GCGGCTGGGG GGCGAGACGG CTACACACTG ACCCTGTACC ACGGGCGGCT GGGCAGCATG 
GTGGCTACCA CCTCTCTTGG GAGAGACACC CACAACTTCA CCTTCACGGG CCTGAACCCA 
GGAAGCAAGT ATGTTCTGGA GGCGGGATCC ATAGCAGGGT CCTACCAGAC ACCTGCAGGG 
AACATCAGCA ACTGGACGTA CCCCCTGGCA CCTCGTAACG TGTACATGAC GAACCAGGGG 
TATCCCAACA GGCTGAGTGT GTCCTGGCGA GCTGAACCCC AAGGGCAAGA CAGTTACAGG 
CTCCTCCTGT ATCATTCGGG ATCAGGTATT CTGACAGCCA ATGTATCTGT TGAGAAAGGC 
ACCAGCAAAT TCACCTTTTC TGGCCTGGCT CCGGGACATA AATACCTGCT GGAAGTGGTG 
TCCACGGCAG GGCCCTACGC AGCCTCTGCA GGGAACATCA GTGACTGGAC AACCCCCTCA 
GTTCCCAAAA ATCTCTCTGC GATGGCTGAA GGGAACAACA CAGTGCTCAT CTCCTGGGAC 
AGTGTCTCTG GACAACAGGA TGAGTGCCAG TTGTGGCTGC GGGATCCCAG GAACAGCACA 
CTGCCCTGGC GGCACGCTCT CAGCCAAGGG CAAGTCCACC ACCTCCTCCA GGGGCTGGTC 
CCAGGGAGGA ACTACTCTGT CTCCTTGAGC TGCGTGGCCG GGCCCTACTG GAGCAGCACC 
AAACCCTTGG CGGTGCTGAT GGAGCCAAAC CCAGTGGAAG ATGTGCAATG CTTATCAGAG 
TCAAGGAGCC TTTACTTGAA CTGGACCAGC TCTCCTGGAG ATGTGGAGGC TTATGAAGTG 
GTGACAGAGA GACTTTCTGA TGGACTTCCC ACCTCCAAGT ATGTCATGAG CATCCCTATG 
AGTGAGGCCA GCCTGGAGGG GCTGCAGCCA AACTCGTCAT ACCGAATCGC TGTGAGCACA 
GTGGGCATGA ACACAATTAG GAGCCAGGCT GTGACTCTGC TCTGCAACAC AACAGTGGAG 
GCCTTGCCTC CGCCCCTGCG AGCAGACATC TTCCAAGTAG AGGCCAGCTC CGCAGTTATC 
ATTTCATCCG ACCTGTTCAG TGAAGAGAAT GGCCAGATTG AGTATTACGG TGTCGTTGCT 
ACCACTAATG ATTCACTGCT GAGGCCCACC CAGGAAATCA TGTCCAGCAC ATGGTATGAC 
CACTATTACG GGACAGAGGA CTCCTACCTG GCTGTGCTAA TCCCCAATCC TTTCCATTCG 
AGCCCCAGGA GCTCCCCCGA AACCTGGCGA GTGCCAGTGG GAATAGAGGA GTGCGGCCAG 
TCCAGGGCAA TGTGCAACGG GAAGCTGAAA GCCAACGAGC AGTACAGGTT CAGCATTGCT 
GCCTTCACCA AATACGACCC AGTAGCCCCT GCAGTGACCT TCACCATGTT CTCAGCTGCT 
GAATCCAGTG CAGATGCAAC TCCACTCTCA ATGCCAATAA TCACTGGAAT CATCGTGGGA 
TTTCTCTTGA CACTTGCAGC TATTTTTGCT TTGGTCTACT GGAAACAGCT CAGAGCAAAG 
AGAACCAACA AAAGCAGCCT GCCCCAGGAA ATGGTGACCT ACAGCTTGAG AAACATCCAT 
CGGCCAATTC CCATACAGAA CTTCAAGCAG TACTATGAGT TGAAGACAGC AAGTGCTAAC 
CACGCTTTTT TCCAGGAGTT TGAGGAGCTG AAGGAAGTTG GGAAGGAGCA GCCAAAGGTG 
GAGGCTGAGC TACCAGCAAA TGTCTCCAAG AACAGATATC CCCACGTGCT GCCCTATGAT 
CACTCTCGGG TCAAGCTGAG CCAACTGGGG GAGGATCCAC ACTCTGACTA CATCAATGCC 
AACTTCATGC CTGGCTATAC ATCCCAGCAG GAGTTCATTG CCACCCAGGG GCCCCTGAAG 
AAAACGATAG AGGACTTCTG GAGGCTGGTG TGGGAGCAGA ATGTCTGCAA CATCATCATG 
CTGACAGTAT GCATGGAGAA CGGGCGGGTT CTCTGTGATC ACTACTGGCC ATCCGAATCT 
GCCCCAGTCT CCTATGGGCA CATCCGTGTC CACTTGCTGT CACAGAGCAG CTCCGAGGAG 
TGGACGATGC GCGAGTTCAA ACTGTGGCAT GAGGGTATCC GGGCAGAGCG GCATGTGTCC 
CACCTGCACT ACACGGCATG GCCTGACCAT GGGATCCCAG AGTCCACGAC TTCCATTATG 
ACCTTCAGAG AGCTGGTCCG GGAGCGTATC CAGAGCACCA AGGACGCGGG GCCAACCCTC 
GTGCACTGCA GCGCTGGGGT GGGCCGCACT GGCACCTTCA TTGCACTGGA CCGGCTGCTG 
CAGCAGATGA AGCAGGAGAA GGTGGTGGAC ACGTTTGGTG TTGTTTACAC CTTGCGGATG 
AACCGTTACC TGATGATTCA GACGCTGTCC CAGTATATTT TCCTGCACAG CTGTATTCTG 
GAAAAGATCT TAGAGGAACC ACTCCTGGGT TTATCAGGGA CAGAGAGGTC CTGCCCCATC 
CCGCTGAAAA GCTTTGCTCA GCACTATGCC CAGAAGGCAA CCAAGTCGCA TGTGGGCTTC 
CTGAGGGAGT ACGAGACCCT CCTAGAGGCT GTCAAGGAAG AAGCCAGCTC TGCAACACCA 
TCTTCAGGCG ACCAGCAGAC ACATCCCTCT TCCAGTATCC TCCCATATGA TCGCTCCAGA 
GTGAAGTTTT CCCTGCTGGA ACAGGGCCCA TTCTCAGGTC TCCTGCAGGT GTGGCGTGTC 
CCGGGCTGCA GCTCCAGCAG GGAATATCTG GCTGTCCAGG GGCCTGACAA GTTGACTGTG 
GAGGACTTCT GGACCCTGGT GTGGGAACAG GACGTTCACA CCATCCTAAC ACTTCTCCCA 
TGGCAGGAGA AGGGGGAGAT CCCAAGTGGG GCGTGCTGGC CCCTGGAAGG AGACTCTCTC 
TGCACAAAGA CGCTGACCAT CCAGTGTGGC ACAGAGAAGC TTGTCTCGGG ATGGAGGTGC 
ATCCAGCTCA AAATGAAACA TGAGAAGAAA GCAAAGGAGA GGCAGGTACA ACAGTTCCTA 
TACACACTGT GGAGCAGCAA GAAACAGCCA GATGTCCAAA GCCTGGTGGA GTTCCTCACT 
GCTGTCAGAC GGTGCATGCC CCACCGAAAG AGAGCCGGTC CTCTCCTGCT GCACTGCAGC 
AGTGGCGTGA GCCAAATGGG GACACTGATC TCCCTGGACT GCCTGTTGCA CCAGATGAAA 
GCTGAGAGAA CGGTGGATGT CTATGGGGTC ACCCTCCAGC TGGCAAGAAG CTGTTGTCTC 
ATGACCCCAA CACTGGACCA GTACATGTTC CTGTACACCT GCATCCGGGA CATCATTGCT 
CAGAAACAGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014801835.1
CDS	200..5734
Translation	MGEGYVLALSPMEHPVKNSSLVKGVTNFTYNGLHPGTLYTFEVSTVAGPYTSSPRRITNWTYPLPLEQLTLSNQGHSTSLQASWRAAPAGSTGYAGTLWETKSQERVRNMTVGNNWMNATFEDLVPGRQYTLEMTAMAGPYRSPVRSATDWTYPLAPAGVTLTNTRRPLGLAAFWDKAAGDVDQFHLQLYSKSHPAQRNISVGPDTHNFTFLGLSPGIQYFLKVTVLAGPYRSSSHFATEWTYPLSLANVSVQPGRRPQELHVSWVESGGGRDHLVQLSVAESLSIIKNVSVPRGVNQLDLEGLVPGSRYRVEIISQAGPHRTSSQTAIGYTVPLPPLSLSASPVSTAWALAVHWETPSGQRDGYLLSVHEEGSSKPARNLKVGKDSTNVTLAQLEPGTCYLVEIWAVAGPYRSLPKNFTGCTVPAAPTDLSLTNPGSSSELYTCWNKPPGRRDRYHVILYGLSIQSRNRVQTLSPDAQNITWTHLEAGSRFAVQVTAVKGSLEASSTNVTQWTYPLAPANLTLGSPSASELQASWATTGRGAEGYVVDVYDRASSSHVGHVVLGGDARSHTFRKLSPGTRYSVAVRATAGPFHTSTPNFTLCTRPLPPAAVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFMGLNPGYKYSLEASAMAGPYHAAAPNISAWTRPLPPASVRLLSTGHPDRLSASWAAAAGGRDGYTLTLYHGRLGSMVATTSLGRDTHNFTFTGLNPGSKYVLEAGSIAGSYQTPAGNISNWTYPLAPRNVYMTNQGYPNRLSVSWRAEPQGQDSYRLLLYHSGSGILTANVSVEKGTSKFTFSGLAPGHKYLLEVVSTAGPYAASAGNISDWTTPSVPKNLSAMAEGNNTVLISWDSVSGQQDECQLWLRDPRNSTLPWRHALSQGQVHHLLQGLVPGRNYSVSLSCVAGPYWSSTKPLAVLMEPNPVEDVQCLSESRSLYLNWTSSPGDVEAYEVVTERLSDGLPTSKYVMSIPMSEASLEGLQPNSSYRIAVSTVGMNTIRSQAVTLLCNTTVEALPPPLRADIFQVEASSAVIISSDLFSEENGQIEYYGVVATTNDSLLRPTQEIMSSTWYDHYYGTEDSYLAVLIPNPFHSSPRSSPETWRVPVGIEECGQSRAMCNGKLKANEQYRFSIAAFTKYDPVAPAVTFTMFSAAESSADATPLSMPIITGIIVGFLLTLAAIFALVYWKQLRAKRTNKSSLPQEMVTYSLRNIHRPIPIQNFKQYYELKTASANHAFFQEFEELKEVGKEQPKVEAELPANVSKNRYPHVLPYDHSRVKLSQLGEDPHSDYINANFMPGYTSQQEFIATQGPLKKTIEDFWRLVWEQNVCNIIMLTVCMENGRVLCDHYWPSESAPVSYGHIRVHLLSQSSSEEWTMREFKLWHEGIRAERHVSHLHYTAWPDHGIPESTTSIMTFRELVRERIQSTKDAGPTLVHCSAGVGRTGTFIALDRLLQQMKQEKVVDTFGVVYTLRMNRYLMIQTLSQYIFLHSCILEKILEEPLLGLSGTERSCPIPLKSFAQHYAQKATKSHVGFLREYETLLEAVKEEASSATPSSGDQQTHPSSSILPYDRSRVKFSLLEQGPFSGLLQVWRVPGCSSSREYLAVQGPDKLTVEDFWTLVWEQDVHTILTLLPWQEKGEIPSGACWPLEGDSLCTKTLTIQCGTEKLVSGWRCIQLKMKHEKKAKERQVQQFLYTLWSSKKQPDVQSLVEFLTAVRRCMPHRKRAGPLLLHCSSGVSQMGTLISLDCLLHQMKAERTVDVYGVTLQLARSCCLMTPTLDQYMFLYTCIRDIIAQKQP

Target ORF information:

RefSeq Version	XM_014946349.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax receptor-type tyrosine-protein phosphatase V-like (LOC106890484), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946349.1

ORF Insert Sequence:

ATGGGAGAGG GCTACGTGCT GGCCCTCAGC CCCATGGAGC ACCCTGTGAA GAATAGCTCG 
CTGGTTAAAG GTGTCACCAA CTTCACCTAC AACGGCCTCC ACCCTGGGAC TCTTTACACC 
TTTGAGGTCA GCACCGTGGC TGGCCCCTAC ACATCCTCGC CCCGGCGCAT CACCAACTGG 
ACATATCCTT TGCCCCTGGA GCAGCTGACC CTCAGCAATC AGGGCCACAG CACCTCTCTG 
CAAGCCTCCT GGAGAGCAGC TCCCGCTGGC AGCACTGGCT ACGCAGGCAC CCTCTGGGAA 
ACCAAGTCCC AGGAGCGGGT CAGGAACATG ACTGTGGGGA ACAACTGGAT GAATGCCACC 
TTTGAGGACC TGGTCCCTGG GCGGCAGTAT ACACTGGAGA TGACTGCTAT GGCCGGACCT 
TACAGATCCC CTGTGCGATC AGCCACAGAC TGGACATACC CTCTGGCTCC AGCCGGCGTG 
ACCCTGACCA ACACCCGACG CCCACTGGGA CTTGCTGCCT TCTGGGACAA GGCTGCTGGT 
GATGTGGACC AGTTCCACCT CCAGCTCTAC AGCAAGAGCC ATCCAGCACA GAGGAACATC 
TCAGTAGGGC CAGACACCCA CAACTTCACA TTCCTAGGGC TGTCCCCTGG GATTCAGTAC 
TTCCTGAAGG TGACTGTCCT CGCTGGCCCA TACAGATCCT CGTCACACTT TGCCACTGAG 
TGGACATATC CACTGTCTCT GGCTAACGTG AGTGTACAGC CTGGCCGGAG ACCTCAGGAG 
CTACATGTGA GCTGGGTGGA GTCAGGTGGT GGCAGAGACC ACTTGGTACA GCTCTCAGTG 
GCCGAGTCCC TATCCATCAT AAAGAATGTG TCTGTCCCCC GTGGAGTCAA CCAGCTTGAC 
CTGGAGGGGC TGGTTCCAGG GTCTCGATAC CGTGTGGAGA TCATTTCTCA GGCTGGGCCT 
CATCGCACCT CCTCTCAGAC TGCCATTGGC TACACTGTCC CACTACCTCC GCTTTCCCTC 
TCGGCAAGCC CTGTCAGCAC TGCCTGGGCT CTGGCTGTGC ACTGGGAGAC CCCTTCTGGG 
CAGAGGGATG GATATCTGCT CAGTGTGCAT GAGGAAGGTT CTTCTAAACC AGCAAGGAAC 
CTGAAAGTAG GGAAAGACAG CACCAATGTC ACCCTGGCAC AGCTGGAACC AGGAACTTGC 
TACCTTGTTG AGATCTGGGC TGTAGCTGGA CCTTACCGCT CACTCCCCAA GAACTTCACT 
GGCTGCACAG TTCCTGCTGC ACCGACAGAC CTGAGCCTCA CCAACCCAGG CAGCTCCTCA 
GAGCTTTACA CATGCTGGAA CAAGCCCCCC GGCAGGAGGG ATCGCTACCA TGTTATCCTG 
TATGGCCTCA GCATCCAGAG TAGGAATCGG GTCCAGACCT TGAGTCCAGA TGCCCAGAAC 
ATCACCTGGA CTCACCTGGA GGCAGGCAGC AGGTTTGCTG TGCAGGTCAC TGCTGTGAAA 
GGTTCATTAG AAGCCTCCTC CACCAACGTC ACCCAATGGA CATATCCCTT GGCCCCTGCC 
AACCTCACCC TGGGCAGCCC TTCTGCCTCC GAGCTGCAGG CCTCCTGGGC AACAACGGGG 
CGAGGAGCAG AAGGCTATGT GGTGGATGTC TATGATAGAG CCTCCAGCAG TCACGTTGGG 
CACGTGGTGC TGGGTGGGGA TGCCCGGAGT CACACCTTCA GAAAGCTGAG CCCTGGTACT 
CGCTACAGCG TGGCAGTGAG GGCCACAGCT GGGCCCTTCC ACACCAGCAC CCCCAACTTC 
ACCCTCTGCA CACGCCCGCT GCCCCCAGCC GCCGTGCGCT TGCTGAGCAC GGGGCACCCC 
GACAGGCTGA GTGCGTCCTG GGCAGCCGCG GCTGGGGGGC GAGACGGCTA CACACTGACC 
CTGTACCACG GGCGGCTGGG CAGCATGGTG GCTACCACCT CTCTTGGGAG AGACACCCAC 
AACTTCACCT TCATGGGTCT GAACCCAGGA TACAAGTACT CCCTGGAGGC CAGTGCCATG 
GCTGGACCAT ACCACGCAGC AGCACCCAAC ATCAGTGCCT GGACACGCCC GCTGCCCCCA 
GCCTCCGTGC GCTTGCTGAG CACGGGGCAC CCCGACAGGC TGAGTGCGTC CTGGGCAGCC 
GCGGCTGGGG GGCGAGACGG CTACACACTG ACCCTGTACC ACGGGCGGCT GGGCAGCATG 
GTGGCTACCA CCTCTCTTGG GAGAGACACC CACAACTTCA CCTTCACGGG CCTGAACCCA 
GGAAGCAAGT ATGTTCTGGA GGCGGGATCC ATAGCAGGGT CCTACCAGAC ACCTGCAGGG 
AACATCAGCA ACTGGACGTA CCCCCTGGCA CCTCGTAACG TGTACATGAC GAACCAGGGG 
TATCCCAACA GGCTGAGTGT GTCCTGGCGA GCTGAACCCC AAGGGCAAGA CAGTTACAGG 
CTCCTCCTGT ATCATTCGGG ATCAGGTATT CTGACAGCCA ATGTATCTGT TGAGAAAGGC 
ACCAGCAAAT TCACCTTTTC TGGCCTGGCT CCGGGACATA AATACCTGCT GGAAGTGGTG 
TCCACGGCAG GGCCCTACGC AGCCTCTGCA GGGAACATCA GTGACTGGAC AACCCCCTCA 
GTTCCCAAAA ATCTCTCTGC GATGGCTGAA GGGAACAACA CAGTGCTCAT CTCCTGGGAC 
AGTGTCTCTG GACAACAGGA TGAGTGCCAG TTGTGGCTGC GGGATCCCAG GAACAGCACA 
CTGCCCTGGC GGCACGCTCT CAGCCAAGGG CAAGTCCACC ACCTCCTCCA GGGGCTGGTC 
CCAGGGAGGA ACTACTCTGT CTCCTTGAGC TGCGTGGCCG GGCCCTACTG GAGCAGCACC 
AAACCCTTGG CGGTGCTGAT GGAGCCAAAC CCAGTGGAAG ATGTGCAATG CTTATCAGAG 
TCAAGGAGCC TTTACTTGAA CTGGACCAGC TCTCCTGGAG ATGTGGAGGC TTATGAAGTG 
GTGACAGAGA GACTTTCTGA TGGACTTCCC ACCTCCAAGT ATGTCATGAG CATCCCTATG 
AGTGAGGCCA GCCTGGAGGG GCTGCAGCCA AACTCGTCAT ACCGAATCGC TGTGAGCACA 
GTGGGCATGA ACACAATTAG GAGCCAGGCT GTGACTCTGC TCTGCAACAC AACAGTGGAG 
GCCTTGCCTC CGCCCCTGCG AGCAGACATC TTCCAAGTAG AGGCCAGCTC CGCAGTTATC 
ATTTCATCCG ACCTGTTCAG TGAAGAGAAT GGCCAGATTG AGTATTACGG TGTCGTTGCT 
ACCACTAATG ATTCACTGCT GAGGCCCACC CAGGAAATCA TGTCCAGCAC ATGGTATGAC 
CACTATTACG GGACAGAGGA CTCCTACCTG GCTGTGCTAA TCCCCAATCC TTTCCATTCG 
AGCCCCAGGA GCTCCCCCGA AACCTGGCGA GTGCCAGTGG GAATAGAGGA GTGCGGCCAG 
TCCAGGGCAA TGTGCAACGG GAAGCTGAAA GCCAACGAGC AGTACAGGTT CAGCATTGCT 
GCCTTCACCA AATACGACCC AGTAGCCCCT GCAGTGACCT TCACCATGTT CTCAGCTGCT 
GAATCCAGTG CAGATGCAAC TCCACTCTCA ATGCCAATAA TCACTGGAAT CATCGTGGGA 
TTTCTCTTGA CACTTGCAGC TATTTTTGCT TTGGTCTACT GGAAACAGCT CAGAGCAAAG 
AGAACCAACA AAAGCAGCCT GCCCCAGGAA ATGGTGACCT ACAGCTTGAG AAACATCCAT 
CGGCCAATTC CCATACAGAA CTTCAAGCAG TACTATGAGT TGAAGACAGC AAGTGCTAAC 
CACGCTTTTT TCCAGGAGTT TGAGGAGCTG AAGGAAGTTG GGAAGGAGCA GCCAAAGGTG 
GAGGCTGAGC TACCAGCAAA TGTCTCCAAG AACAGATATC CCCACGTGCT GCCCTATGAT 
CACTCTCGGG TCAAGCTGAG CCAACTGGGG GAGGATCCAC ACTCTGACTA CATCAATGCC 
AACTTCATGC CTGGCTATAC ATCCCAGCAG GAGTTCATTG CCACCCAGGG GCCCCTGAAG 
AAAACGATAG AGGACTTCTG GAGGCTGGTG TGGGAGCAGA ATGTCTGCAA CATCATCATG 
CTGACAGTAT GCATGGAGAA CGGGCGGGTT CTCTGTGATC ACTACTGGCC ATCCGAATCT 
GCCCCAGTCT CCTATGGGCA CATCCGTGTC CACTTGCTGT CACAGAGCAG CTCCGAGGAG 
TGGACGATGC GCGAGTTCAA ACTGTGGCAT GAGGGTATCC GGGCAGAGCG GCATGTGTCC 
CACCTGCACT ACACGGCATG GCCTGACCAT GGGATCCCAG AGTCCACGAC TTCCATTATG 
ACCTTCAGAG AGCTGGTCCG GGAGCGTATC CAGAGCACCA AGGACGCGGG GCCAACCCTC 
GTGCACTGCA GCGCTGGGGT GGGCCGCACT GGCACCTTCA TTGCACTGGA CCGGCTGCTG 
CAGCAGATGA AGCAGGAGAA GGTGGTGGAC ACGTTTGGTG TTGTTTACAC CTTGCGGATG 
AACCGTTACC TGATGATTCA GACGCTGTCC CAGTATATTT TCCTGCACAG CTGTATTCTG 
GAAAAGATCT TAGAGGAACC ACTCCTGGGT TTATCAGGGA CAGAGAGGTC CTGCCCCATC 
CCGCTGAAAA GCTTTGCTCA GCACTATGCC CAGAAGGCAA CCAAGTCGCA TGTGGGCTTC 
CTGAGGGAGT ACGAGACCCT CCTAGAGGCT GTCAAGGAAG AAGCCAGCTC TGCAACACCA 
TCTTCAGGCG ACCAGCAGAC ACATCCCTCT TCCAGTATCC TCCCATATGA TCGCTCCAGA 
GTGAAGTTTT CCCTGCTGGA ACAGGGCCCA TTCTCAGGTC TCCTGCAGGT GTGGCGTGTC 
CCGGGCTGCA GCTCCAGCAG GGAATATCTG GCTGTCCAGG GGCCTGACAA GTTGACTGTG 
GAGGACTTCT GGACCCTGGT GTGGGAACAG GACGTTCACA CCATCCTAAC ACTTCTCCCA 
TGGCAGGAGA AGGGGGAGAT CCCAAGTGGG GCGTGCTGGC CCCTGGAAGG AGACTCTCTC 
TGCACAAAGA CGCTGACCAT CCAGTGTGGC ACAGAGAAGC TTGTCTCGGG ATGGAGGTGC 
ATCCAGCTCA AAATGAAACA TGAGAAGAAA GCAAAGGAGA GGCAGGTACA ACAGTTCCTA 
TACACACTGT GGAGCAGCAA GAAACAGCCA GATGTCCAAA GCCTGGTGGA GTTCCTCACT 
GCTGTCAGAC GGTGCATGCC CCACCGAAAG AGAGCCGGTC CTCTCCTGCT GCACTGCAGC 
AGTGGCGTGA GCCAAATGGG GACACTGATC TCCCTGGACT GCCTGTTGCA CCAGATGAAA 
GCTGAGAGAA CGGTGGATGT CTATGGGGTC ACCCTCCAGC TGGCAAGAAG CTGTTGTCTC 
ATGACCCCAA CACTGGACCA GTACATGTTC CTGTACACCT GCATCCGGGA CATCATTGCT 
CAGAAACAGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC106890484 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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