MITF cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol MITF

Entrez Gene ID 106890754

Full Name melanogenesis associated transcription factor

Gene Type protein-coding

Organism Calidris pugnax(ruff)

mRNA	Protein	Name
XM_014946745.1	XP_014802231.1	microphthalmia-associated transcription factor isoform X2
XM_014946750.1	XP_014802236.1	microphthalmia-associated transcription factor isoform X6
XM_014946752.1	XP_014802238.1	microphthalmia-associated transcription factor isoform X7
XM_014946748.1	XP_014802234.1	microphthalmia-associated transcription factor isoform X4
XM_014946747.1	XP_014802233.1	microphthalmia-associated transcription factor isoform X3
XM_014946749.1	XP_014802235.1	microphthalmia-associated transcription factor isoform X5
XM_014946744.1	XP_014802230.1	microphthalmia-associated transcription factor isoform X1
XM_014946751.1	XP_014802237.1	microphthalmia-associated transcription factor isoform X7

Pathways from BioSystems
Gene Ontology

The following MITF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MITF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa68524	XM_014946749.1 Latest version!	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCa68525	XM_014946750.1 Latest version!	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCa68526	XM_014946751.1 Latest version!	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCa68526	XM_014946752.1 Latest version!	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCa68520	XM_014946744.1 Latest version!	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa68521	XM_014946745.1 Latest version!	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa68522	XM_014946747.1 Latest version!	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa68523	XM_014946748.1 Latest version!	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MITF ( XM_014946749.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014802235.1 Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCa68524
Clone ID Related Accession (Same CDS sequence)	XM_014946749.1
Accession Version	XM_014946749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microphthalmia-associated transcription factor isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGG AGTCGGGGAT CGTGCCGGAC TTCGAGGTGG GCGAGGAGTT CCACGAGGAG 
CCCAAGACCT ACTACGAGCT GAAGAGCCAG CCCCTCAAGA GCAGCAATTC CGAGCATCCT 
GGCGCTTCCA AGCCTCCCTT AAGCTCCTCC AGCATGACCT CACGAATCCT GCTACGCCAG 
CAGCTCATGC GTGAGCAGAT GCAGGAGCAG GAGCGGCGGG AGCAGCAGCA GAAGCAGCAG 
GCAGCCCAGT TCATGCAGCA GCGAGTGCCC GTCAGCCAGA CACCTGCCAT CAACGTCAGC 
GTCCCCACCA GCCTGCCCCC CGCCACCCAG GTACCCATGG AGGTCCTCAA GGTGCAGACT 
CACCTTGAGA ATCCAACCAA GTACCATATT CAGCAAGCCC AGCGGCAGCA GGTAAAGCAG 
TACCTCTCTA CCACCCTAGC AAACAAACAT GCCAACCAGG CCCTGAGCTT GCCGTGCCCC 
AACCAGCCCG GTGATCATGT CATGCCGCCT GGAACTGGAA GCAGCGCACC CAACAGCCCC 
ATGGCTATGC TCACCCTCAA CTCCAACTGT GAAAAAGAGA TGGATGATGT GATTGATGAC 
ATAATAAGTC TGGAATCCAG TTACAATGAA GAAATCCTTG GCTTGATGGA TCCAGCCCTG 
CAAATGGCAA ATACGTTGCC TGTGTCTGGC AATTTGATTG ATCTTTATGG CAACCAAAGC 
ATGCCTCCTC CGGGACTAAA CATCAGCAAC TCATGTCCAG CTAATCTTCC TAATATAAAA 
AGGGAGCTCA CAGCATGTAT TTTTCCTACA GAGTCAGAAG CGAGAGCGTT GGCTAAGGAG 
AGGCAAAAGA AGGACAATCA CAACTTGATT GAACGAAGAA GAAGATTTAA TATTAATGAC 
CGTATAAAAG AACTAGGCAC TTTGATACCC AAGTCAAATG ACCCGGATAT GCGCTGGAAT 
AAGGGAACTA TTCTGAAGGC ATCAGTGGAC TATATCCGTA AGCTGCAGAG AGAACAGCAA 
CGCACCAAGG AGCTTGAGAA CAGGCAGAAG AAGTTGGAAC ACGCCAACAG GCACCTGCTG 
CTCAGAATAC AGGAGCTTGA GATGCAAGCT CGGGCACACG GACTGTCCCT TGTTCCCTCT 
ACGGGCCTTT GCTCCCCTGA TATGGTCAAC AGGGTCATCA AACAAGAGCC CGTGCTGGAC 
AACTGCAACC AAGACATGAT GGCGCACCAC ACAGACCTGT CTTGCACTAC CACCCTGGAC 
CTCACTGACG GTACCATCGC CTTCAGCGAC AACCTCGGAA ACGTGACTGA ACCCACCGGC 
ACTTACAGTG TTCCTGCAAA AATGGGATCC AAACTGGAAG ATATCTTGAT GGACGATACC 
CTCTCCCCCG TGGGAGTAAC TGACCCCCTT CTTTCCTCTG TGTCCCCTGG AGCCTCCAAG 
ACGAGTAGCA GGAGGAGCAG CGTGAGCATG GAGGACACCG ATCACGCTTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014802235.1
CDS	83..1576
Translation	MQAESGIVPDFEVGEEFHEEPKTYYELKSQPLKSSNSEHPGASKPPLSSSSMTSRILLRQQLMREQMQEQERREQQQKQQAAQFMQQRVPVSQTPAINVSVPTSLPPATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQALSLPCPNQPGDHVMPPGTGSSAPNSPMAMLTLNSNCEKEMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQSMPPPGLNISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRTKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLVPSTGLCSPDMVNRVIKQEPVLDNCNQDMMAHHTDLSCTTTLDLTDGTIAFSDNLGNVTEPTGTYSVPAKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSVSMEDTDHAC

Target ORF information:

RefSeq Version	XM_014946749.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946749.1

ORF Insert Sequence:

ATGCAGGCGG AGTCGGGGAT CGTGCCGGAC TTCGAGGTGG GCGAGGAGTT CCACGAGGAG 
CCCAAGACCT ACTACGAGCT GAAGAGCCAG CCCCTCAAGA GCAGCAATTC CGAGCATCCT 
GGCGCTTCCA AGCCTCCCTT AAGCTCCTCC AGCATGACCT CACGAATCCT GCTACGCCAG 
CAGCTCATGC GTGAGCAGAT GCAGGAGCAG GAGCGGCGGG AGCAGCAGCA GAAGCAGCAG 
GCAGCCCAGT TCATGCAGCA GCGAGTGCCC GTCAGCCAGA CACCTGCCAT CAACGTCAGC 
GTCCCCACCA GCCTGCCCCC CGCCACCCAG GTACCCATGG AGGTCCTCAA GGTGCAGACT 
CACCTTGAGA ATCCAACCAA GTACCATATT CAGCAAGCCC AGCGGCAGCA GGTAAAGCAG 
TACCTCTCTA CCACCCTAGC AAACAAACAT GCCAACCAGG CCCTGAGCTT GCCGTGCCCC 
AACCAGCCCG GTGATCATGT CATGCCGCCT GGAACTGGAA GCAGCGCACC CAACAGCCCC 
ATGGCTATGC TCACCCTCAA CTCCAACTGT GAAAAAGAGA TGGATGATGT GATTGATGAC 
ATAATAAGTC TGGAATCCAG TTACAATGAA GAAATCCTTG GCTTGATGGA TCCAGCCCTG 
CAAATGGCAA ATACGTTGCC TGTGTCTGGC AATTTGATTG ATCTTTATGG CAACCAAAGC 
ATGCCTCCTC CGGGACTAAA CATCAGCAAC TCATGTCCAG CTAATCTTCC TAATATAAAA 
AGGGAGCTCA CAGCATGTAT TTTTCCTACA GAGTCAGAAG CGAGAGCGTT GGCTAAGGAG 
AGGCAAAAGA AGGACAATCA CAACTTGATT GAACGAAGAA GAAGATTTAA TATTAATGAC 
CGTATAAAAG AACTAGGCAC TTTGATACCC AAGTCAAATG ACCCGGATAT GCGCTGGAAT 
AAGGGAACTA TTCTGAAGGC ATCAGTGGAC TATATCCGTA AGCTGCAGAG AGAACAGCAA 
CGCACCAAGG AGCTTGAGAA CAGGCAGAAG AAGTTGGAAC ACGCCAACAG GCACCTGCTG 
CTCAGAATAC AGGAGCTTGA GATGCAAGCT CGGGCACACG GACTGTCCCT TGTTCCCTCT 
ACGGGCCTTT GCTCCCCTGA TATGGTCAAC AGGGTCATCA AACAAGAGCC CGTGCTGGAC 
AACTGCAACC AAGACATGAT GGCGCACCAC ACAGACCTGT CTTGCACTAC CACCCTGGAC 
CTCACTGACG GTACCATCGC CTTCAGCGAC AACCTCGGAA ACGTGACTGA ACCCACCGGC 
ACTTACAGTG TTCCTGCAAA AATGGGATCC AAACTGGAAG ATATCTTGAT GGACGATACC 
CTCTCCCCCG TGGGAGTAAC TGACCCCCTT CTTTCCTCTG TGTCCCCTGG AGCCTCCAAG 
ACGAGTAGCA GGAGGAGCAG CGTGAGCATG GAGGACACCG ATCACGCTTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MITF ( XM_014946750.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014802236.1 Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCa68525
Clone ID Related Accession (Same CDS sequence)	XM_014946750.1
Accession Version	XM_014946750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microphthalmia-associated transcription factor isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGG AGTCGGGGAT CGTGCCGGAC TTCGAGGTGG GCGAGGAGTT CCACGAGGAG 
CCCAAGACCT ACTACGAGCT GAAGAGCCAG CCCCTCAAGA GCAGCAATTC CGAGCATCCT 
GGCGCTTCCA AGCCTCCCTT AAGCTCCTCC AGCATGACCT CACGAATCCT GCTACGCCAG 
CAGCTCATGC GTGAGCAGAT GCAGGAGCAG GAGCGGCGGG AGCAGCAGCA GAAGCAGCAG 
GCAGCCCAGT TCATGCAGCA GCGAGTGCCC GTCAGCCAGA CACCTGCCAT CAACGTCAGC 
GTCCCCACCA GCCTGCCCCC CGCCACCCAG GTACCCATGG AGGTCCTCAA GGTGCAGACT 
CACCTTGAGA ATCCAACCAA GTACCATATT CAGCAAGCCC AGCGGCAGCA GGTAAAGCAG 
TACCTCTCTA CCACCCTAGC AAACAAACAT GCCAACCAGG CCCTGAGCTT GCCGTGCCCC 
AACCAGCCCG GTGATCATGT CATGCCGCCT GGAACTGGAA GCAGCGCACC CAACAGCCCC 
ATGGCTATGC TCACCCTCAA CTCCAACTGT GAAAAAGAGA TGGATGATGT GATTGATGAC 
ATAATAAGTC TGGAATCCAG TTACAATGAA GAAATCCTTG GCTTGATGGA TCCAGCCCTG 
CAAATGGCAA ATACGTTGCC TGTGTCTGGC AATTTGATTG ATCTTTATGG CAACCAAAGC 
ATGCCTCCTC CGGGACTAAA CATCAGCAAC TCATGTCCAG CTAATCTTCC TAATATAAAA 
AGGGAGCTCA CAGAGTCAGA AGCGAGAGCG TTGGCTAAGG AGAGGCAAAA GAAGGACAAT 
CACAACTTGA TTGAACGAAG AAGAAGATTT AATATTAATG ACCGTATAAA AGAACTAGGC 
ACTTTGATAC CCAAGTCAAA TGACCCGGAT ATGCGCTGGA ATAAGGGAAC TATTCTGAAG 
GCATCAGTGG ACTATATCCG TAAGCTGCAG AGAGAACAGC AACGCACCAA GGAGCTTGAG 
AACAGGCAGA AGAAGTTGGA ACACGCCAAC AGGCACCTGC TGCTCAGAAT ACAGGAGCTT 
GAGATGCAAG CTCGGGCACA CGGACTGTCC CTTGTTCCCT CTACGGGCCT TTGCTCCCCT 
GATATGGTCA ACAGGGTCAT CAAACAAGAG CCCGTGCTGG ACAACTGCAA CCAAGACATG 
ATGGCGCACC ACACAGACCT GTCTTGCACT ACCACCCTGG ACCTCACTGA CGGTACCATC 
GCCTTCAGCG ACAACCTCGG AAACGTGACT GAACCCACCG GCACTTACAG TGTTCCTGCA 
AAAATGGGAT CCAAACTGGA AGATATCTTG ATGGACGATA CCCTCTCCCC CGTGGGAGTA 
ACTGACCCCC TTCTTTCCTC TGTGTCCCCT GGAGCCTCCA AGACGAGTAG CAGGAGGAGC 
AGCGTGAGCA TGGAGGACAC CGATCACGCT TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014802236.1
CDS	83..1558
Translation	MQAESGIVPDFEVGEEFHEEPKTYYELKSQPLKSSNSEHPGASKPPLSSSSMTSRILLRQQLMREQMQEQERREQQQKQQAAQFMQQRVPVSQTPAINVSVPTSLPPATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQALSLPCPNQPGDHVMPPGTGSSAPNSPMAMLTLNSNCEKEMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQSMPPPGLNISNSCPANLPNIKRELTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRTKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLVPSTGLCSPDMVNRVIKQEPVLDNCNQDMMAHHTDLSCTTTLDLTDGTIAFSDNLGNVTEPTGTYSVPAKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSVSMEDTDHAC

Target ORF information:

RefSeq Version	XM_014946750.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946750.1

ORF Insert Sequence:

ATGCAGGCGG AGTCGGGGAT CGTGCCGGAC TTCGAGGTGG GCGAGGAGTT CCACGAGGAG 
CCCAAGACCT ACTACGAGCT GAAGAGCCAG CCCCTCAAGA GCAGCAATTC CGAGCATCCT 
GGCGCTTCCA AGCCTCCCTT AAGCTCCTCC AGCATGACCT CACGAATCCT GCTACGCCAG 
CAGCTCATGC GTGAGCAGAT GCAGGAGCAG GAGCGGCGGG AGCAGCAGCA GAAGCAGCAG 
GCAGCCCAGT TCATGCAGCA GCGAGTGCCC GTCAGCCAGA CACCTGCCAT CAACGTCAGC 
GTCCCCACCA GCCTGCCCCC CGCCACCCAG GTACCCATGG AGGTCCTCAA GGTGCAGACT 
CACCTTGAGA ATCCAACCAA GTACCATATT CAGCAAGCCC AGCGGCAGCA GGTAAAGCAG 
TACCTCTCTA CCACCCTAGC AAACAAACAT GCCAACCAGG CCCTGAGCTT GCCGTGCCCC 
AACCAGCCCG GTGATCATGT CATGCCGCCT GGAACTGGAA GCAGCGCACC CAACAGCCCC 
ATGGCTATGC TCACCCTCAA CTCCAACTGT GAAAAAGAGA TGGATGATGT GATTGATGAC 
ATAATAAGTC TGGAATCCAG TTACAATGAA GAAATCCTTG GCTTGATGGA TCCAGCCCTG 
CAAATGGCAA ATACGTTGCC TGTGTCTGGC AATTTGATTG ATCTTTATGG CAACCAAAGC 
ATGCCTCCTC CGGGACTAAA CATCAGCAAC TCATGTCCAG CTAATCTTCC TAATATAAAA 
AGGGAGCTCA CAGAGTCAGA AGCGAGAGCG TTGGCTAAGG AGAGGCAAAA GAAGGACAAT 
CACAACTTGA TTGAACGAAG AAGAAGATTT AATATTAATG ACCGTATAAA AGAACTAGGC 
ACTTTGATAC CCAAGTCAAA TGACCCGGAT ATGCGCTGGA ATAAGGGAAC TATTCTGAAG 
GCATCAGTGG ACTATATCCG TAAGCTGCAG AGAGAACAGC AACGCACCAA GGAGCTTGAG 
AACAGGCAGA AGAAGTTGGA ACACGCCAAC AGGCACCTGC TGCTCAGAAT ACAGGAGCTT 
GAGATGCAAG CTCGGGCACA CGGACTGTCC CTTGTTCCCT CTACGGGCCT TTGCTCCCCT 
GATATGGTCA ACAGGGTCAT CAAACAAGAG CCCGTGCTGG ACAACTGCAA CCAAGACATG 
ATGGCGCACC ACACAGACCT GTCTTGCACT ACCACCCTGG ACCTCACTGA CGGTACCATC 
GCCTTCAGCG ACAACCTCGG AAACGTGACT GAACCCACCG GCACTTACAG TGTTCCTGCA 
AAAATGGGAT CCAAACTGGA AGATATCTTG ATGGACGATA CCCTCTCCCC CGTGGGAGTA 
ACTGACCCCC TTCTTTCCTC TGTGTCCCCT GGAGCCTCCA AGACGAGTAG CAGGAGGAGC 
AGCGTGAGCA TGGAGGACAC CGATCACGCT TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MITF ( XM_014946752.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014802238.1 Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCa68526
Clone ID Related Accession (Same CDS sequence)	XM_014946752.1 , XM_014946751.1
Accession Version	XM_014946752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microphthalmia-associated transcription factor isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCAC GAATCCTGCT ACGCCAGCAG CTCATGCGTG AGCAGATGCA GGAGCAGGAG 
CGGCGGGAGC AGCAGCAGAA GCAGCAGGCA GCCCAGTTCA TGCAGCAGCG AGTGCCCGTC 
AGCCAGACAC CTGCCATCAA CGTCAGCGTC CCCACCAGCC TGCCCCCCGC CACCCAGGTA 
CCCATGGAGG TCCTCAAGGT GCAGACTCAC CTTGAGAATC CAACCAAGTA CCATATTCAG 
CAAGCCCAGC GGCAGCAGGT AAAGCAGTAC CTCTCTACCA CCCTAGCAAA CAAACATGCC 
AACCAGGCCC TGAGCTTGCC GTGCCCCAAC CAGCCCGGTG ATCATGTCAT GCCGCCTGGA 
ACTGGAAGCA GCGCACCCAA CAGCCCCATG GCTATGCTCA CCCTCAACTC CAACTGTGAA 
AAAGAGGGAT TTTATAAATT TGAAGAGCAA AGCAGGGTTG AGAGTGAGTG CCCGGCTCTG 
AATACACACT CACGAGCATC ATGCATGCAG ATGGATGATG TGATTGATGA CATAATAAGT 
CTGGAATCCA GTTACAATGA AGAAATCCTT GGCTTGATGG ATCCAGCCCT GCAAATGGCA 
AATACGTTGC CTGTGTCTGG CAATTTGATT GATCTTTATG GCAACCAAAG CATGCCTCCT 
CCGGGACTAA ACATCAGCAA CTCATGTCCA GCTAATCTTC CTAATATAAA AAGGGAGCTC 
ACAGCATGTA TTTTTCCTAC AGAGTCAGAA GCGAGAGCGT TGGCTAAGGA GAGGCAAAAG 
AAGGACAATC ACAACTTGAT TGAACGAAGA AGAAGATTTA ATATTAATGA CCGTATAAAA 
GAACTAGGCA CTTTGATACC CAAGTCAAAT GACCCGGATA TGCGCTGGAA TAAGGGAACT 
ATTCTGAAGG CATCAGTGGA CTATATCCGT AAGCTGCAGA GAGAACAGCA ACGCACCAAG 
GAGCTTGAGA ACAGGCAGAA GAAGTTGGAA CACGCCAACA GGCACCTGCT GCTCAGAATA 
CAGGAGCTTG AGATGCAAGC TCGGGCACAC GGACTGTCCC TTGTTCCCTC TACGGGCCTT 
TGCTCCCCTG ATATGGTCAA CAGGGTCATC AAACAAGAGC CCGTGCTGGA CAACTGCAAC 
CAAGACATGA TGGCGCACCA CACAGACCTG TCTTGCACTA CCACCCTGGA CCTCACTGAC 
GGTACCATCG CCTTCAGCGA CAACCTCGGA AACGTGACTG AACCCACCGG CACTTACAGT 
GTTCCTGCAA AAATGGGATC CAAACTGGAA GATATCTTGA TGGACGATAC CCTCTCCCCC 
GTGGGAGTAA CTGACCCCCT TCTTTCCTCT GTGTCCCCTG GAGCCTCCAA GACGAGTAGC 
AGGAGGAGCA GCGTGAGCAT GGAGGACACC GATCACGCTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014802238.1
CDS	355..1779
Translation	MTSRILLRQQLMREQMQEQERREQQQKQQAAQFMQQRVPVSQTPAINVSVPTSLPPATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQALSLPCPNQPGDHVMPPGTGSSAPNSPMAMLTLNSNCEKEGFYKFEEQSRVESECPALNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQSMPPPGLNISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRTKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLVPSTGLCSPDMVNRVIKQEPVLDNCNQDMMAHHTDLSCTTTLDLTDGTIAFSDNLGNVTEPTGTYSVPAKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSVSMEDTDHAC

Target ORF information:

RefSeq Version	XM_014946752.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946752.1

ORF Insert Sequence:

ATGACCTCAC GAATCCTGCT ACGCCAGCAG CTCATGCGTG AGCAGATGCA GGAGCAGGAG 
CGGCGGGAGC AGCAGCAGAA GCAGCAGGCA GCCCAGTTCA TGCAGCAGCG AGTGCCCGTC 
AGCCAGACAC CTGCCATCAA CGTCAGCGTC CCCACCAGCC TGCCCCCCGC CACCCAGGTA 
CCCATGGAGG TCCTCAAGGT GCAGACTCAC CTTGAGAATC CAACCAAGTA CCATATTCAG 
CAAGCCCAGC GGCAGCAGGT AAAGCAGTAC CTCTCTACCA CCCTAGCAAA CAAACATGCC 
AACCAGGCCC TGAGCTTGCC GTGCCCCAAC CAGCCCGGTG ATCATGTCAT GCCGCCTGGA 
ACTGGAAGCA GCGCACCCAA CAGCCCCATG GCTATGCTCA CCCTCAACTC CAACTGTGAA 
AAAGAGGGAT TTTATAAATT TGAAGAGCAA AGCAGGGTTG AGAGTGAGTG CCCGGCTCTG 
AATACACACT CACGAGCATC ATGCATGCAG ATGGATGATG TGATTGATGA CATAATAAGT 
CTGGAATCCA GTTACAATGA AGAAATCCTT GGCTTGATGG ATCCAGCCCT GCAAATGGCA 
AATACGTTGC CTGTGTCTGG CAATTTGATT GATCTTTATG GCAACCAAAG CATGCCTCCT 
CCGGGACTAA ACATCAGCAA CTCATGTCCA GCTAATCTTC CTAATATAAA AAGGGAGCTC 
ACAGCATGTA TTTTTCCTAC AGAGTCAGAA GCGAGAGCGT TGGCTAAGGA GAGGCAAAAG 
AAGGACAATC ACAACTTGAT TGAACGAAGA AGAAGATTTA ATATTAATGA CCGTATAAAA 
GAACTAGGCA CTTTGATACC CAAGTCAAAT GACCCGGATA TGCGCTGGAA TAAGGGAACT 
ATTCTGAAGG CATCAGTGGA CTATATCCGT AAGCTGCAGA GAGAACAGCA ACGCACCAAG 
GAGCTTGAGA ACAGGCAGAA GAAGTTGGAA CACGCCAACA GGCACCTGCT GCTCAGAATA 
CAGGAGCTTG AGATGCAAGC TCGGGCACAC GGACTGTCCC TTGTTCCCTC TACGGGCCTT 
TGCTCCCCTG ATATGGTCAA CAGGGTCATC AAACAAGAGC CCGTGCTGGA CAACTGCAAC 
CAAGACATGA TGGCGCACCA CACAGACCTG TCTTGCACTA CCACCCTGGA CCTCACTGAC 
GGTACCATCG CCTTCAGCGA CAACCTCGGA AACGTGACTG AACCCACCGG CACTTACAGT 
GTTCCTGCAA AAATGGGATC CAAACTGGAA GATATCTTGA TGGACGATAC CCTCTCCCCC 
GTGGGAGTAA CTGACCCCCT TCTTTCCTCT GTGTCCCCTG GAGCCTCCAA GACGAGTAGC 
AGGAGGAGCA GCGTGAGCAT GGAGGACACC GATCACGCTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MITF ( XM_014946751.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014802237.1 Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCa68526
Clone ID Related Accession (Same CDS sequence)	XM_014946752.1 , XM_014946751.1
Accession Version	XM_014946751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microphthalmia-associated transcription factor isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCAC GAATCCTGCT ACGCCAGCAG CTCATGCGTG AGCAGATGCA GGAGCAGGAG 
CGGCGGGAGC AGCAGCAGAA GCAGCAGGCA GCCCAGTTCA TGCAGCAGCG AGTGCCCGTC 
AGCCAGACAC CTGCCATCAA CGTCAGCGTC CCCACCAGCC TGCCCCCCGC CACCCAGGTA 
CCCATGGAGG TCCTCAAGGT GCAGACTCAC CTTGAGAATC CAACCAAGTA CCATATTCAG 
CAAGCCCAGC GGCAGCAGGT AAAGCAGTAC CTCTCTACCA CCCTAGCAAA CAAACATGCC 
AACCAGGCCC TGAGCTTGCC GTGCCCCAAC CAGCCCGGTG ATCATGTCAT GCCGCCTGGA 
ACTGGAAGCA GCGCACCCAA CAGCCCCATG GCTATGCTCA CCCTCAACTC CAACTGTGAA 
AAAGAGGGAT TTTATAAATT TGAAGAGCAA AGCAGGGTTG AGAGTGAGTG CCCGGCTCTG 
AATACACACT CACGAGCATC ATGCATGCAG ATGGATGATG TGATTGATGA CATAATAAGT 
CTGGAATCCA GTTACAATGA AGAAATCCTT GGCTTGATGG ATCCAGCCCT GCAAATGGCA 
AATACGTTGC CTGTGTCTGG CAATTTGATT GATCTTTATG GCAACCAAAG CATGCCTCCT 
CCGGGACTAA ACATCAGCAA CTCATGTCCA GCTAATCTTC CTAATATAAA AAGGGAGCTC 
ACAGCATGTA TTTTTCCTAC AGAGTCAGAA GCGAGAGCGT TGGCTAAGGA GAGGCAAAAG 
AAGGACAATC ACAACTTGAT TGAACGAAGA AGAAGATTTA ATATTAATGA CCGTATAAAA 
GAACTAGGCA CTTTGATACC CAAGTCAAAT GACCCGGATA TGCGCTGGAA TAAGGGAACT 
ATTCTGAAGG CATCAGTGGA CTATATCCGT AAGCTGCAGA GAGAACAGCA ACGCACCAAG 
GAGCTTGAGA ACAGGCAGAA GAAGTTGGAA CACGCCAACA GGCACCTGCT GCTCAGAATA 
CAGGAGCTTG AGATGCAAGC TCGGGCACAC GGACTGTCCC TTGTTCCCTC TACGGGCCTT 
TGCTCCCCTG ATATGGTCAA CAGGGTCATC AAACAAGAGC CCGTGCTGGA CAACTGCAAC 
CAAGACATGA TGGCGCACCA CACAGACCTG TCTTGCACTA CCACCCTGGA CCTCACTGAC 
GGTACCATCG CCTTCAGCGA CAACCTCGGA AACGTGACTG AACCCACCGG CACTTACAGT 
GTTCCTGCAA AAATGGGATC CAAACTGGAA GATATCTTGA TGGACGATAC CCTCTCCCCC 
GTGGGAGTAA CTGACCCCCT TCTTTCCTCT GTGTCCCCTG GAGCCTCCAA GACGAGTAGC 
AGGAGGAGCA GCGTGAGCAT GGAGGACACC GATCACGCTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014802237.1
CDS	450..1874
Translation	MTSRILLRQQLMREQMQEQERREQQQKQQAAQFMQQRVPVSQTPAINVSVPTSLPPATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQALSLPCPNQPGDHVMPPGTGSSAPNSPMAMLTLNSNCEKEGFYKFEEQSRVESECPALNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQSMPPPGLNISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRTKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLVPSTGLCSPDMVNRVIKQEPVLDNCNQDMMAHHTDLSCTTTLDLTDGTIAFSDNLGNVTEPTGTYSVPAKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSVSMEDTDHAC

Target ORF information:

RefSeq Version	XM_014946751.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946751.1

ORF Insert Sequence:

ATGACCTCAC GAATCCTGCT ACGCCAGCAG CTCATGCGTG AGCAGATGCA GGAGCAGGAG 
CGGCGGGAGC AGCAGCAGAA GCAGCAGGCA GCCCAGTTCA TGCAGCAGCG AGTGCCCGTC 
AGCCAGACAC CTGCCATCAA CGTCAGCGTC CCCACCAGCC TGCCCCCCGC CACCCAGGTA 
CCCATGGAGG TCCTCAAGGT GCAGACTCAC CTTGAGAATC CAACCAAGTA CCATATTCAG 
CAAGCCCAGC GGCAGCAGGT AAAGCAGTAC CTCTCTACCA CCCTAGCAAA CAAACATGCC 
AACCAGGCCC TGAGCTTGCC GTGCCCCAAC CAGCCCGGTG ATCATGTCAT GCCGCCTGGA 
ACTGGAAGCA GCGCACCCAA CAGCCCCATG GCTATGCTCA CCCTCAACTC CAACTGTGAA 
AAAGAGGGAT TTTATAAATT TGAAGAGCAA AGCAGGGTTG AGAGTGAGTG CCCGGCTCTG 
AATACACACT CACGAGCATC ATGCATGCAG ATGGATGATG TGATTGATGA CATAATAAGT 
CTGGAATCCA GTTACAATGA AGAAATCCTT GGCTTGATGG ATCCAGCCCT GCAAATGGCA 
AATACGTTGC CTGTGTCTGG CAATTTGATT GATCTTTATG GCAACCAAAG CATGCCTCCT 
CCGGGACTAA ACATCAGCAA CTCATGTCCA GCTAATCTTC CTAATATAAA AAGGGAGCTC 
ACAGCATGTA TTTTTCCTAC AGAGTCAGAA GCGAGAGCGT TGGCTAAGGA GAGGCAAAAG 
AAGGACAATC ACAACTTGAT TGAACGAAGA AGAAGATTTA ATATTAATGA CCGTATAAAA 
GAACTAGGCA CTTTGATACC CAAGTCAAAT GACCCGGATA TGCGCTGGAA TAAGGGAACT 
ATTCTGAAGG CATCAGTGGA CTATATCCGT AAGCTGCAGA GAGAACAGCA ACGCACCAAG 
GAGCTTGAGA ACAGGCAGAA GAAGTTGGAA CACGCCAACA GGCACCTGCT GCTCAGAATA 
CAGGAGCTTG AGATGCAAGC TCGGGCACAC GGACTGTCCC TTGTTCCCTC TACGGGCCTT 
TGCTCCCCTG ATATGGTCAA CAGGGTCATC AAACAAGAGC CCGTGCTGGA CAACTGCAAC 
CAAGACATGA TGGCGCACCA CACAGACCTG TCTTGCACTA CCACCCTGGA CCTCACTGAC 
GGTACCATCG CCTTCAGCGA CAACCTCGGA AACGTGACTG AACCCACCGG CACTTACAGT 
GTTCCTGCAA AAATGGGATC CAAACTGGAA GATATCTTGA TGGACGATAC CCTCTCCCCC 
GTGGGAGTAA CTGACCCCCT TCTTTCCTCT GTGTCCCCTG GAGCCTCCAA GACGAGTAGC 
AGGAGGAGCA GCGTGAGCAT GGAGGACACC GATCACGCTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MITF ( XM_014946744.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014802230.1 Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa68520
Clone ID Related Accession (Same CDS sequence)	XM_014946744.1
Accession Version	XM_014946744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microphthalmia-associated transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGG AGTCGGGGAT CGTGCCGGAC TTCGAGGTGG GCGAGGAGTT CCACGAGGAG 
CCCAAGACCT ACTACGAGCT GAAGAGCCAG CCCCTCAAGA GCAGCAATTC CGAGCATCCT 
GGCGCTTCCA AGCCTCCCTT AAGCTCCTCC AGCATGACCT CACGAATCCT GCTACGCCAG 
CAGCTCATGC GTGAGCAGAT GCAGGAGCAG GAGCGGCGGG AGCAGCAGCA GAAGCAGCAG 
GCAGCCCAGT TCATGCAGCA GCGAGTGCCC GTCAGCCAGA CACCTGCCAT CAACGTCAGC 
GTCCCCACCA GCCTGCCCCC CGCCACCCAG GTACCCATGG AGGTCCTCAA GGTGCAGACT 
CACCTTGAGA ATCCAACCAA GTACCATATT CAGCAAGCCC AGCGGCAGCA GGTAAAGCAG 
TACCTCTCTA CCACCCTAGC AAACAAACAT GCCAACCAGG CCCTGAGCTT GCCGTGCCCC 
AACCAGCCCG GTGATCATGT CATGCCGCCT GGAACTGGAA GCAGCGCACC CAACAGCCCC 
ATGGCTATGC TCACCCTCAA CTCCAACTGT GAAAAAGAGG GATTTTATAA ATTTGAAGAG 
CAAAGCAGGG TTGAGAGTGA GTGCCCGGCT CTGAATACAC ACTCACGAGC ATCATGCATG 
CAGATGGATG ATGTGATTGA TGACATAATA AGTCTGGAAT CCAGTTACAA TGAAGAAATC 
CTTGGCTTGA TGGATCCAGC CCTGCAAATG GCAAATACGT TGCCTGTGTC TGGCAATTTG 
ATTGATCTTT ATGGCAACCA AAGCATGCCT CCTCCGGGAC TAAACATCAG CAACTCATGT 
CCAGCTAATC TTCCTAATAT AAAAAGGGAG CTCACAGCAT GTATTTTTCC TACAGAGTCA 
GAAGCGAGAG CGTTGGCTAA GGAGAGGCAA AAGAAGGACA ATCACAACTT GATTGAACGA 
AGAAGAAGAT TTAATATTAA TGACCGTATA AAAGAACTAG GCACTTTGAT ACCCAAGTCA 
AATGACCCGG ATATGCGCTG GAATAAGGGA ACTATTCTGA AGGCATCAGT GGACTATATC 
CGTAAGCTGC AGAGAGAACA GCAACGCACC AAGGAGCTTG AGAACAGGCA GAAGAAGTTG 
GAACACGCCA ACAGGCACCT GCTGCTCAGA ATACAGGAGC TTGAGATGCA AGCTCGGGCA 
CACGGACTGT CCCTTGTTCC CTCTACGGGC CTTTGCTCCC CTGATATGGT CAACAGGGTC 
ATCAAACAAG AGCCCGTGCT GGACAACTGC AACCAAGACA TGATGGCGCA CCACACAGAC 
CTGTCTTGCA CTACCACCCT GGACCTCACT GACGGTACCA TCGCCTTCAG CGACAACCTC 
GGAAACGTGA CTGAACCCAC CGGCACTTAC AGTGTTCCTG CAAAAATGGG ATCCAAACTG 
GAAGATATCT TGATGGACGA TACCCTCTCC CCCGTGGGAG TAACTGACCC CCTTCTTTCC 
TCTGTGTCCC CTGGAGCCTC CAAGACGAGT AGCAGGAGGA GCAGCGTGAG CATGGAGGAC 
ACCGATCACG CTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014802230.1
CDS	83..1660
Translation	MQAESGIVPDFEVGEEFHEEPKTYYELKSQPLKSSNSEHPGASKPPLSSSSMTSRILLRQQLMREQMQEQERREQQQKQQAAQFMQQRVPVSQTPAINVSVPTSLPPATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQALSLPCPNQPGDHVMPPGTGSSAPNSPMAMLTLNSNCEKEGFYKFEEQSRVESECPALNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQSMPPPGLNISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRTKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLVPSTGLCSPDMVNRVIKQEPVLDNCNQDMMAHHTDLSCTTTLDLTDGTIAFSDNLGNVTEPTGTYSVPAKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSVSMEDTDHAC

Target ORF information:

RefSeq Version	XM_014946744.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946744.1

ORF Insert Sequence:

ATGCAGGCGG AGTCGGGGAT CGTGCCGGAC TTCGAGGTGG GCGAGGAGTT CCACGAGGAG 
CCCAAGACCT ACTACGAGCT GAAGAGCCAG CCCCTCAAGA GCAGCAATTC CGAGCATCCT 
GGCGCTTCCA AGCCTCCCTT AAGCTCCTCC AGCATGACCT CACGAATCCT GCTACGCCAG 
CAGCTCATGC GTGAGCAGAT GCAGGAGCAG GAGCGGCGGG AGCAGCAGCA GAAGCAGCAG 
GCAGCCCAGT TCATGCAGCA GCGAGTGCCC GTCAGCCAGA CACCTGCCAT CAACGTCAGC 
GTCCCCACCA GCCTGCCCCC CGCCACCCAG GTACCCATGG AGGTCCTCAA GGTGCAGACT 
CACCTTGAGA ATCCAACCAA GTACCATATT CAGCAAGCCC AGCGGCAGCA GGTAAAGCAG 
TACCTCTCTA CCACCCTAGC AAACAAACAT GCCAACCAGG CCCTGAGCTT GCCGTGCCCC 
AACCAGCCCG GTGATCATGT CATGCCGCCT GGAACTGGAA GCAGCGCACC CAACAGCCCC 
ATGGCTATGC TCACCCTCAA CTCCAACTGT GAAAAAGAGG GATTTTATAA ATTTGAAGAG 
CAAAGCAGGG TTGAGAGTGA GTGCCCGGCT CTGAATACAC ACTCACGAGC ATCATGCATG 
CAGATGGATG ATGTGATTGA TGACATAATA AGTCTGGAAT CCAGTTACAA TGAAGAAATC 
CTTGGCTTGA TGGATCCAGC CCTGCAAATG GCAAATACGT TGCCTGTGTC TGGCAATTTG 
ATTGATCTTT ATGGCAACCA AAGCATGCCT CCTCCGGGAC TAAACATCAG CAACTCATGT 
CCAGCTAATC TTCCTAATAT AAAAAGGGAG CTCACAGCAT GTATTTTTCC TACAGAGTCA 
GAAGCGAGAG CGTTGGCTAA GGAGAGGCAA AAGAAGGACA ATCACAACTT GATTGAACGA 
AGAAGAAGAT TTAATATTAA TGACCGTATA AAAGAACTAG GCACTTTGAT ACCCAAGTCA 
AATGACCCGG ATATGCGCTG GAATAAGGGA ACTATTCTGA AGGCATCAGT GGACTATATC 
CGTAAGCTGC AGAGAGAACA GCAACGCACC AAGGAGCTTG AGAACAGGCA GAAGAAGTTG 
GAACACGCCA ACAGGCACCT GCTGCTCAGA ATACAGGAGC TTGAGATGCA AGCTCGGGCA 
CACGGACTGT CCCTTGTTCC CTCTACGGGC CTTTGCTCCC CTGATATGGT CAACAGGGTC 
ATCAAACAAG AGCCCGTGCT GGACAACTGC AACCAAGACA TGATGGCGCA CCACACAGAC 
CTGTCTTGCA CTACCACCCT GGACCTCACT GACGGTACCA TCGCCTTCAG CGACAACCTC 
GGAAACGTGA CTGAACCCAC CGGCACTTAC AGTGTTCCTG CAAAAATGGG ATCCAAACTG 
GAAGATATCT TGATGGACGA TACCCTCTCC CCCGTGGGAG TAACTGACCC CCTTCTTTCC 
TCTGTGTCCC CTGGAGCCTC CAAGACGAGT AGCAGGAGGA GCAGCGTGAG CATGGAGGAC 
ACCGATCACG CTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MITF ( XM_014946745.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014802231.1 Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa68521
Clone ID Related Accession (Same CDS sequence)	XM_014946745.1
Accession Version	XM_014946745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microphthalmia-associated transcription factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGG AGTCGGGGAT CGTGCCGGAC TTCGAGGTGG GCGAGGAGTT CCACGAGGAG 
CCCAAGACCT ACTACGAGCT GAAGAGCCAG CCCCTCAAGA GCAGCAATTC CGAGCATCCT 
GGCGCTTCCA AGCCTCCCTT AAGCTCCTCC AGCATGACCT CACGAATCCT GCTACGCCAG 
CAGCTCATGC GTGAGCAGAT GCAGGAGCAG GAGCGGCGGG AGCAGCAGCA GAAGCAGCAG 
GCAGCCCAGT TCATGCAGCA GCGAGTGCCC GTCAGCCAGA CACCTGCCAT CAACGTCAGC 
GTCCCCACCA GCCTGCCCCC CGCCACCCAG GTACCCATGG AGGTCCTCAA GGTGCAGACT 
CACCTTGAGA ATCCAACCAA GTACCATATT CAGCAAGCCC AGCGGCAGCA GGTAAAGCAG 
TACCTCTCTA CCACCCTAGC AAACAAACAT GCCAACCAGG CCCTGAGCTT GCCGTGCCCC 
AACCAGCCCG GTGATCATGT CATGCCGCCT GGAACTGGAA GCAGCGCACC CAACAGCCCC 
ATGGCTATGC TCACCCTCAA CTCCAACTGT GAAAAAGAGG GATTTTATAA ATTTGAAGAG 
CAAAGCAGGG TTGAGAGTGA GTGCCCGGCT CTGAATACAC ACTCACGAGC ATCATGCATG 
CAGATGGATG ATGTGATTGA TGACATAATA AGTCTGGAAT CCAGTTACAA TGAAGAAATC 
CTTGGCTTGA TGGATCCAGC CCTGCAAATG GCAAATACGT TGCCTGTGTC TGGCAATTTG 
ATTGATCTTT ATGGCAACCA AAGCATGCCT CCTCCGGGAC TAAACATCAG CAACTCATGT 
CCAGCTAATC TTCCTAATAT AAAAAGGGAG CTCACAGAGT CAGAAGCGAG AGCGTTGGCT 
AAGGAGAGGC AAAAGAAGGA CAATCACAAC TTGATTGAAC GAAGAAGAAG ATTTAATATT 
AATGACCGTA TAAAAGAACT AGGCACTTTG ATACCCAAGT CAAATGACCC GGATATGCGC 
TGGAATAAGG GAACTATTCT GAAGGCATCA GTGGACTATA TCCGTAAGCT GCAGAGAGAA 
CAGCAACGCA CCAAGGAGCT TGAGAACAGG CAGAAGAAGT TGGAACACGC CAACAGGCAC 
CTGCTGCTCA GAATACAGGA GCTTGAGATG CAAGCTCGGG CACACGGACT GTCCCTTGTT 
CCCTCTACGG GCCTTTGCTC CCCTGATATG GTCAACAGGG TCATCAAACA AGAGCCCGTG 
CTGGACAACT GCAACCAAGA CATGATGGCG CACCACACAG ACCTGTCTTG CACTACCACC 
CTGGACCTCA CTGACGGTAC CATCGCCTTC AGCGACAACC TCGGAAACGT GACTGAACCC 
ACCGGCACTT ACAGTGTTCC TGCAAAAATG GGATCCAAAC TGGAAGATAT CTTGATGGAC 
GATACCCTCT CCCCCGTGGG AGTAACTGAC CCCCTTCTTT CCTCTGTGTC CCCTGGAGCC 
TCCAAGACGA GTAGCAGGAG GAGCAGCGTG AGCATGGAGG ACACCGATCA CGCTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014802231.1
CDS	83..1642
Translation	MQAESGIVPDFEVGEEFHEEPKTYYELKSQPLKSSNSEHPGASKPPLSSSSMTSRILLRQQLMREQMQEQERREQQQKQQAAQFMQQRVPVSQTPAINVSVPTSLPPATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQALSLPCPNQPGDHVMPPGTGSSAPNSPMAMLTLNSNCEKEGFYKFEEQSRVESECPALNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQSMPPPGLNISNSCPANLPNIKRELTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRTKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLVPSTGLCSPDMVNRVIKQEPVLDNCNQDMMAHHTDLSCTTTLDLTDGTIAFSDNLGNVTEPTGTYSVPAKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSVSMEDTDHAC

Target ORF information:

RefSeq Version	XM_014946745.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946745.1

ORF Insert Sequence:

ATGCAGGCGG AGTCGGGGAT CGTGCCGGAC TTCGAGGTGG GCGAGGAGTT CCACGAGGAG 
CCCAAGACCT ACTACGAGCT GAAGAGCCAG CCCCTCAAGA GCAGCAATTC CGAGCATCCT 
GGCGCTTCCA AGCCTCCCTT AAGCTCCTCC AGCATGACCT CACGAATCCT GCTACGCCAG 
CAGCTCATGC GTGAGCAGAT GCAGGAGCAG GAGCGGCGGG AGCAGCAGCA GAAGCAGCAG 
GCAGCCCAGT TCATGCAGCA GCGAGTGCCC GTCAGCCAGA CACCTGCCAT CAACGTCAGC 
GTCCCCACCA GCCTGCCCCC CGCCACCCAG GTACCCATGG AGGTCCTCAA GGTGCAGACT 
CACCTTGAGA ATCCAACCAA GTACCATATT CAGCAAGCCC AGCGGCAGCA GGTAAAGCAG 
TACCTCTCTA CCACCCTAGC AAACAAACAT GCCAACCAGG CCCTGAGCTT GCCGTGCCCC 
AACCAGCCCG GTGATCATGT CATGCCGCCT GGAACTGGAA GCAGCGCACC CAACAGCCCC 
ATGGCTATGC TCACCCTCAA CTCCAACTGT GAAAAAGAGG GATTTTATAA ATTTGAAGAG 
CAAAGCAGGG TTGAGAGTGA GTGCCCGGCT CTGAATACAC ACTCACGAGC ATCATGCATG 
CAGATGGATG ATGTGATTGA TGACATAATA AGTCTGGAAT CCAGTTACAA TGAAGAAATC 
CTTGGCTTGA TGGATCCAGC CCTGCAAATG GCAAATACGT TGCCTGTGTC TGGCAATTTG 
ATTGATCTTT ATGGCAACCA AAGCATGCCT CCTCCGGGAC TAAACATCAG CAACTCATGT 
CCAGCTAATC TTCCTAATAT AAAAAGGGAG CTCACAGAGT CAGAAGCGAG AGCGTTGGCT 
AAGGAGAGGC AAAAGAAGGA CAATCACAAC TTGATTGAAC GAAGAAGAAG ATTTAATATT 
AATGACCGTA TAAAAGAACT AGGCACTTTG ATACCCAAGT CAAATGACCC GGATATGCGC 
TGGAATAAGG GAACTATTCT GAAGGCATCA GTGGACTATA TCCGTAAGCT GCAGAGAGAA 
CAGCAACGCA CCAAGGAGCT TGAGAACAGG CAGAAGAAGT TGGAACACGC CAACAGGCAC 
CTGCTGCTCA GAATACAGGA GCTTGAGATG CAAGCTCGGG CACACGGACT GTCCCTTGTT 
CCCTCTACGG GCCTTTGCTC CCCTGATATG GTCAACAGGG TCATCAAACA AGAGCCCGTG 
CTGGACAACT GCAACCAAGA CATGATGGCG CACCACACAG ACCTGTCTTG CACTACCACC 
CTGGACCTCA CTGACGGTAC CATCGCCTTC AGCGACAACC TCGGAAACGT GACTGAACCC 
ACCGGCACTT ACAGTGTTCC TGCAAAAATG GGATCCAAAC TGGAAGATAT CTTGATGGAC 
GATACCCTCT CCCCCGTGGG AGTAACTGAC CCCCTTCTTT CCTCTGTGTC CCCTGGAGCC 
TCCAAGACGA GTAGCAGGAG GAGCAGCGTG AGCATGGAGG ACACCGATCA CGCTTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MITF ( XM_014946747.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014802233.1 Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCa68522
Clone ID Related Accession (Same CDS sequence)	XM_014946747.1
Accession Version	XM_014946747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microphthalmia-associated transcription factor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTG TTAGAGTTCA GATGTTCATG CCCCCCCGCT CCTTTGACAG CTTCTGTCTC 
AATTCCGAGC ATCCTGGCGC TTCCAAGCCT CCCTTAAGCT CCTCCAGCAT GACCTCACGA 
ATCCTGCTAC GCCAGCAGCT CATGCGTGAG CAGATGCAGG AGCAGGAGCG GCGGGAGCAG 
CAGCAGAAGC AGCAGGCAGC CCAGTTCATG CAGCAGCGAG TGCCCGTCAG CCAGACACCT 
GCCATCAACG TCAGCGTCCC CACCAGCCTG CCCCCCGCCA CCCAGGTACC CATGGAGGTC 
CTCAAGGTGC AGACTCACCT TGAGAATCCA ACCAAGTACC ATATTCAGCA AGCCCAGCGG 
CAGCAGGTAA AGCAGTACCT CTCTACCACC CTAGCAAACA AACATGCCAA CCAGGCCCTG 
AGCTTGCCGT GCCCCAACCA GCCCGGTGAT CATGTCATGC CGCCTGGAAC TGGAAGCAGC 
GCACCCAACA GCCCCATGGC TATGCTCACC CTCAACTCCA ACTGTGAAAA AGAGGGATTT 
TATAAATTTG AAGAGCAAAG CAGGGTTGAG AGTGAGTGCC CGGCTCTGAA TACACACTCA 
CGAGCATCAT GCATGCAGAT GGATGATGTG ATTGATGACA TAATAAGTCT GGAATCCAGT 
TACAATGAAG AAATCCTTGG CTTGATGGAT CCAGCCCTGC AAATGGCAAA TACGTTGCCT 
GTGTCTGGCA ATTTGATTGA TCTTTATGGC AACCAAAGCA TGCCTCCTCC GGGACTAAAC 
ATCAGCAACT CATGTCCAGC TAATCTTCCT AATATAAAAA GGGAGCTCAC AGCATGTATT 
TTTCCTACAG AGTCAGAAGC GAGAGCGTTG GCTAAGGAGA GGCAAAAGAA GGACAATCAC 
AACTTGATTG AACGAAGAAG AAGATTTAAT ATTAATGACC GTATAAAAGA ACTAGGCACT 
TTGATACCCA AGTCAAATGA CCCGGATATG CGCTGGAATA AGGGAACTAT TCTGAAGGCA 
TCAGTGGACT ATATCCGTAA GCTGCAGAGA GAACAGCAAC GCACCAAGGA GCTTGAGAAC 
AGGCAGAAGA AGTTGGAACA CGCCAACAGG CACCTGCTGC TCAGAATACA GGAGCTTGAG 
ATGCAAGCTC GGGCACACGG ACTGTCCCTT GTTCCCTCTA CGGGCCTTTG CTCCCCTGAT 
ATGGTCAACA GGGTCATCAA ACAAGAGCCC GTGCTGGACA ACTGCAACCA AGACATGATG 
GCGCACCACA CAGACCTGTC TTGCACTACC ACCCTGGACC TCACTGACGG TACCATCGCC 
TTCAGCGACA ACCTCGGAAA CGTGACTGAA CCCACCGGCA CTTACAGTGT TCCTGCAAAA 
ATGGGATCCA AACTGGAAGA TATCTTGATG GACGATACCC TCTCCCCCGT GGGAGTAACT 
GACCCCCTTC TTTCCTCTGT GTCCCCTGGA GCCTCCAAGA CGAGTAGCAG GAGGAGCAGC 
GTGAGCATGG AGGACACCGA TCACGCTTGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014802233.1
CDS	56..1588
Translation	MEAVRVQMFMPPRSFDSFCLNSEHPGASKPPLSSSSMTSRILLRQQLMREQMQEQERREQQQKQQAAQFMQQRVPVSQTPAINVSVPTSLPPATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQALSLPCPNQPGDHVMPPGTGSSAPNSPMAMLTLNSNCEKEGFYKFEEQSRVESECPALNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQSMPPPGLNISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRTKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLVPSTGLCSPDMVNRVIKQEPVLDNCNQDMMAHHTDLSCTTTLDLTDGTIAFSDNLGNVTEPTGTYSVPAKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSVSMEDTDHAC

Target ORF information:

RefSeq Version	XM_014946747.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946747.1

ORF Insert Sequence:

ATGGAAGCTG TTAGAGTTCA GATGTTCATG CCCCCCCGCT CCTTTGACAG CTTCTGTCTC 
AATTCCGAGC ATCCTGGCGC TTCCAAGCCT CCCTTAAGCT CCTCCAGCAT GACCTCACGA 
ATCCTGCTAC GCCAGCAGCT CATGCGTGAG CAGATGCAGG AGCAGGAGCG GCGGGAGCAG 
CAGCAGAAGC AGCAGGCAGC CCAGTTCATG CAGCAGCGAG TGCCCGTCAG CCAGACACCT 
GCCATCAACG TCAGCGTCCC CACCAGCCTG CCCCCCGCCA CCCAGGTACC CATGGAGGTC 
CTCAAGGTGC AGACTCACCT TGAGAATCCA ACCAAGTACC ATATTCAGCA AGCCCAGCGG 
CAGCAGGTAA AGCAGTACCT CTCTACCACC CTAGCAAACA AACATGCCAA CCAGGCCCTG 
AGCTTGCCGT GCCCCAACCA GCCCGGTGAT CATGTCATGC CGCCTGGAAC TGGAAGCAGC 
GCACCCAACA GCCCCATGGC TATGCTCACC CTCAACTCCA ACTGTGAAAA AGAGGGATTT 
TATAAATTTG AAGAGCAAAG CAGGGTTGAG AGTGAGTGCC CGGCTCTGAA TACACACTCA 
CGAGCATCAT GCATGCAGAT GGATGATGTG ATTGATGACA TAATAAGTCT GGAATCCAGT 
TACAATGAAG AAATCCTTGG CTTGATGGAT CCAGCCCTGC AAATGGCAAA TACGTTGCCT 
GTGTCTGGCA ATTTGATTGA TCTTTATGGC AACCAAAGCA TGCCTCCTCC GGGACTAAAC 
ATCAGCAACT CATGTCCAGC TAATCTTCCT AATATAAAAA GGGAGCTCAC AGCATGTATT 
TTTCCTACAG AGTCAGAAGC GAGAGCGTTG GCTAAGGAGA GGCAAAAGAA GGACAATCAC 
AACTTGATTG AACGAAGAAG AAGATTTAAT ATTAATGACC GTATAAAAGA ACTAGGCACT 
TTGATACCCA AGTCAAATGA CCCGGATATG CGCTGGAATA AGGGAACTAT TCTGAAGGCA 
TCAGTGGACT ATATCCGTAA GCTGCAGAGA GAACAGCAAC GCACCAAGGA GCTTGAGAAC 
AGGCAGAAGA AGTTGGAACA CGCCAACAGG CACCTGCTGC TCAGAATACA GGAGCTTGAG 
ATGCAAGCTC GGGCACACGG ACTGTCCCTT GTTCCCTCTA CGGGCCTTTG CTCCCCTGAT 
ATGGTCAACA GGGTCATCAA ACAAGAGCCC GTGCTGGACA ACTGCAACCA AGACATGATG 
GCGCACCACA CAGACCTGTC TTGCACTACC ACCCTGGACC TCACTGACGG TACCATCGCC 
TTCAGCGACA ACCTCGGAAA CGTGACTGAA CCCACCGGCA CTTACAGTGT TCCTGCAAAA 
ATGGGATCCA AACTGGAAGA TATCTTGATG GACGATACCC TCTCCCCCGT GGGAGTAACT 
GACCCCCTTC TTTCCTCTGT GTCCCCTGGA GCCTCCAAGA CGAGTAGCAG GAGGAGCAGC 
GTGAGCATGG AGGACACCGA TCACGCTTGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MITF ( XM_014946748.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014802234.1 Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X4, mRNA.

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CloneID	OCa68523
Clone ID Related Accession (Same CDS sequence)	XM_014946748.1
Accession Version	XM_014946748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	microphthalmia-associated transcription factor isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTG TTAGAGTTCA GATGTTCATG CCCCCCCGCT CCTTTGACAG CTTCTGTCTC 
AATTCCGAGC ATCCTGGCGC TTCCAAGCCT CCCTTAAGCT CCTCCAGCAT GACCTCACGA 
ATCCTGCTAC GCCAGCAGCT CATGCGTGAG CAGATGCAGG AGCAGGAGCG GCGGGAGCAG 
CAGCAGAAGC AGCAGGCAGC CCAGTTCATG CAGCAGCGAG TGCCCGTCAG CCAGACACCT 
GCCATCAACG TCAGCGTCCC CACCAGCCTG CCCCCCGCCA CCCAGGTACC CATGGAGGTC 
CTCAAGGTGC AGACTCACCT TGAGAATCCA ACCAAGTACC ATATTCAGCA AGCCCAGCGG 
CAGCAGGTAA AGCAGTACCT CTCTACCACC CTAGCAAACA AACATGCCAA CCAGGCCCTG 
AGCTTGCCGT GCCCCAACCA GCCCGGTGAT CATGTCATGC CGCCTGGAAC TGGAAGCAGC 
GCACCCAACA GCCCCATGGC TATGCTCACC CTCAACTCCA ACTGTGAAAA AGAGGGATTT 
TATAAATTTG AAGAGCAAAG CAGGGTTGAG AGTGAGTGCC CGGCTCTGAA TACACACTCA 
CGAGCATCAT GCATGCAGAT GGATGATGTG ATTGATGACA TAATAAGTCT GGAATCCAGT 
TACAATGAAG AAATCCTTGG CTTGATGGAT CCAGCCCTGC AAATGGCAAA TACGTTGCCT 
GTGTCTGGCA ATTTGATTGA TCTTTATGGC AACCAAAGCA TGCCTCCTCC GGGACTAAAC 
ATCAGCAACT CATGTCCAGC TAATCTTCCT AATATAAAAA GGGAGCTCAC AGAGTCAGAA 
GCGAGAGCGT TGGCTAAGGA GAGGCAAAAG AAGGACAATC ACAACTTGAT TGAACGAAGA 
AGAAGATTTA ATATTAATGA CCGTATAAAA GAACTAGGCA CTTTGATACC CAAGTCAAAT 
GACCCGGATA TGCGCTGGAA TAAGGGAACT ATTCTGAAGG CATCAGTGGA CTATATCCGT 
AAGCTGCAGA GAGAACAGCA ACGCACCAAG GAGCTTGAGA ACAGGCAGAA GAAGTTGGAA 
CACGCCAACA GGCACCTGCT GCTCAGAATA CAGGAGCTTG AGATGCAAGC TCGGGCACAC 
GGACTGTCCC TTGTTCCCTC TACGGGCCTT TGCTCCCCTG ATATGGTCAA CAGGGTCATC 
AAACAAGAGC CCGTGCTGGA CAACTGCAAC CAAGACATGA TGGCGCACCA CACAGACCTG 
TCTTGCACTA CCACCCTGGA CCTCACTGAC GGTACCATCG CCTTCAGCGA CAACCTCGGA 
AACGTGACTG AACCCACCGG CACTTACAGT GTTCCTGCAA AAATGGGATC CAAACTGGAA 
GATATCTTGA TGGACGATAC CCTCTCCCCC GTGGGAGTAA CTGACCCCCT TCTTTCCTCT 
GTGTCCCCTG GAGCCTCCAA GACGAGTAGC AGGAGGAGCA GCGTGAGCAT GGAGGACACC 
GATCACGCTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014802234.1
CDS	54..1568
Translation	MEAVRVQMFMPPRSFDSFCLNSEHPGASKPPLSSSSMTSRILLRQQLMREQMQEQERREQQQKQQAAQFMQQRVPVSQTPAINVSVPTSLPPATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQALSLPCPNQPGDHVMPPGTGSSAPNSPMAMLTLNSNCEKEGFYKFEEQSRVESECPALNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQSMPPPGLNISNSCPANLPNIKRELTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRTKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLVPSTGLCSPDMVNRVIKQEPVLDNCNQDMMAHHTDLSCTTTLDLTDGTIAFSDNLGNVTEPTGTYSVPAKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSVSMEDTDHAC

Target ORF information:

RefSeq Version	XM_014946748.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax microphthalmia-associated transcription factor (MITF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014946748.1

ORF Insert Sequence:

ATGGAAGCTG TTAGAGTTCA GATGTTCATG CCCCCCCGCT CCTTTGACAG CTTCTGTCTC 
AATTCCGAGC ATCCTGGCGC TTCCAAGCCT CCCTTAAGCT CCTCCAGCAT GACCTCACGA 
ATCCTGCTAC GCCAGCAGCT CATGCGTGAG CAGATGCAGG AGCAGGAGCG GCGGGAGCAG 
CAGCAGAAGC AGCAGGCAGC CCAGTTCATG CAGCAGCGAG TGCCCGTCAG CCAGACACCT 
GCCATCAACG TCAGCGTCCC CACCAGCCTG CCCCCCGCCA CCCAGGTACC CATGGAGGTC 
CTCAAGGTGC AGACTCACCT TGAGAATCCA ACCAAGTACC ATATTCAGCA AGCCCAGCGG 
CAGCAGGTAA AGCAGTACCT CTCTACCACC CTAGCAAACA AACATGCCAA CCAGGCCCTG 
AGCTTGCCGT GCCCCAACCA GCCCGGTGAT CATGTCATGC CGCCTGGAAC TGGAAGCAGC 
GCACCCAACA GCCCCATGGC TATGCTCACC CTCAACTCCA ACTGTGAAAA AGAGGGATTT 
TATAAATTTG AAGAGCAAAG CAGGGTTGAG AGTGAGTGCC CGGCTCTGAA TACACACTCA 
CGAGCATCAT GCATGCAGAT GGATGATGTG ATTGATGACA TAATAAGTCT GGAATCCAGT 
TACAATGAAG AAATCCTTGG CTTGATGGAT CCAGCCCTGC AAATGGCAAA TACGTTGCCT 
GTGTCTGGCA ATTTGATTGA TCTTTATGGC AACCAAAGCA TGCCTCCTCC GGGACTAAAC 
ATCAGCAACT CATGTCCAGC TAATCTTCCT AATATAAAAA GGGAGCTCAC AGAGTCAGAA 
GCGAGAGCGT TGGCTAAGGA GAGGCAAAAG AAGGACAATC ACAACTTGAT TGAACGAAGA 
AGAAGATTTA ATATTAATGA CCGTATAAAA GAACTAGGCA CTTTGATACC CAAGTCAAAT 
GACCCGGATA TGCGCTGGAA TAAGGGAACT ATTCTGAAGG CATCAGTGGA CTATATCCGT 
AAGCTGCAGA GAGAACAGCA ACGCACCAAG GAGCTTGAGA ACAGGCAGAA GAAGTTGGAA 
CACGCCAACA GGCACCTGCT GCTCAGAATA CAGGAGCTTG AGATGCAAGC TCGGGCACAC 
GGACTGTCCC TTGTTCCCTC TACGGGCCTT TGCTCCCCTG ATATGGTCAA CAGGGTCATC 
AAACAAGAGC CCGTGCTGGA CAACTGCAAC CAAGACATGA TGGCGCACCA CACAGACCTG 
TCTTGCACTA CCACCCTGGA CCTCACTGAC GGTACCATCG CCTTCAGCGA CAACCTCGGA 
AACGTGACTG AACCCACCGG CACTTACAGT GTTCCTGCAA AAATGGGATC CAAACTGGAA 
GATATCTTGA TGGACGATAC CCTCTCCCCC GTGGGAGTAA CTGACCCCCT TCTTTCCTCT 
GTGTCCCCTG GAGCCTCCAA GACGAGTAGC AGGAGGAGCA GCGTGAGCAT GGAGGACACC 
GATCACGCTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MITF cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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