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EPHB2 cDNA ORF clone, Calidris pugnax(ruff)

Summary

Gene Symbol EPHB2
Entrez Gene ID 106890862
Full Name EPH receptor B2
Gene Type protein-coding
Organism Calidris pugnax(ruff)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_014946937.1 XP_014802423.1 ephrin type-B receptor 2 isoform X3
XM_014946938.1 XP_014802424.1 ephrin type-B receptor 2 isoform X4
XM_014946935.1 XP_014802421.1 ephrin type-B receptor 2 isoform X1
XM_014946936.1 XP_014802422.1 ephrin type-B receptor 2 isoform X2

Pathways from BioSystems


Gene Ontology

The following EPHB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPHB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa68664 XM_014946935.1
Latest version!		 Calidris pugnax EPH receptor B2 (EPHB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCa68665 XM_014946936.1
Latest version!		 Calidris pugnax EPH receptor B2 (EPHB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCa68666 XM_014946937.1
Latest version!		 Calidris pugnax EPH receptor B2 (EPHB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCa68667 XM_014946938.1
Latest version!		 Calidris pugnax EPH receptor B2 (EPHB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCa68664
Clone ID Related Accession (Same CDS sequence) XM_014946935.1
Accession Version XM_014946935.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-12-08
Organism Calidris pugnax(ruff)
Product ephrin type-B receptor 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGACTCCA CAACGGCCAC GGCAGAGCTG GGCTGGACGG TACATCCTCC TTCGGGGTGG 
GAAGAGGTGA GTGGGTACGA CGAGAACATG AACACCATTC GCACCTACCA GGTGTGCAAC 
GTCTTTGAAT CCAGCCAAAA CAACTGGCTC CGGACCAAGT ACATCCGGAG GCGAGGAGCA 
CACCGCATCC ACGTGGAGAT GAAATTTTCT GTCCGGGACT GCAGCAGCAT CCCTAATGTA 
CCAGGCTCCT GTAAGGAGAC TTTTAACCTC TATTATTTCG AGTCAGACTT TGACTCGGCC 
ACCAAGACTT TTCCTAACTG GATGGAGAAT CCTTGGATGA AGGTGGACAC GATTGCTGCC 
GATGAGAGCT TCTCACAGGT GGACCTAGGT GGACGGGTGA TGAAGATTAA CACTGAGGTG 
CGCAGTTTTG GGCCTGTGTC CAAGAATGGT TTCTACCTGG CCTTCCAGGA CTACGGGGGC 
TGCATGTCTT TGATTGCTGT CCGTGTCTTT TACCGCAAGT GTCCCCGCGT GATCCAGAAT 
GGGGCCGTCT TCCAAGAAAC CCTCTCGGGA GCAGAGAGCA CCTCTCTGGT GGCCGCCCGG 
GGGACGTGCA TCACCAACGC GGAGGAGGTG GACGTGCCCA TCAAGCTGTA CTGCAACGGG 
GATGGCGAGT GGTTGGTGCC CATTGGCCGC TGCATGTGCA GGGCGGGCTA CGAGTCGGTG 
GAAAATGGGA CCGTCTGCAG AGGCTGCCCG TCGGGGACGT TCAAGGCCAG CCAAGGGGAT 
GAAGGATGCG TCCACTGCCC AATTAACAGC CGGACAACTT CAGAAGGGGC CACTAACTGC 
GTGTGCCGAA ACGGATATTA CCGGGCAGAT GCCGATCCTG TCGACATGCC ATGCACCACC 
ATCCCGTCTG CCCCCCAGGC CGTAATCTCC AGTGTGAATG AGACCTCGCT GATGCTGGAG 
TGGACCCCAC CGCGGGACTC AGGGGGCCGG GAGGACCTGG TGTACAACAT CATCTGCAAG 
AGCTGCGGCT CTGGCCGCGG GGCGTGCACA CGCTGTGGGG ACAACGTGCA GTTTGCCCCC 
CGCCAGCTGG GCTTGACAGA GCCCCGCATC TACATCAGCG ACCTGTTGGC CCACACCCAG 
TACACCTTTG AGATCCAGGC GGTGAATGGT GTCACCGACC AGAGCCCCTT CTCCCCGCAG 
TTTGCATCAG TGAACATCAC CACCAACCAG GCTGCTCCTT CAGCTGTGTC CATCATGCAC 
CAGGTCAGCC GCACCGTGGA CAGCATTACC CTCTCGTGGT CTCAGCCTGA CCAACCCAAC 
GGAGTCATCC TGGATTACGA GCTGCAATAT TATGAGAAGG ATCTGAGTGA GTTAAATTCA 
ACAACAGTGA AGAGCCCCAC CAACACTGTG GCAGTGCAAA ACCTCAAAGC TGGCACCATC 
TACGTCTTCC AGGTGCGGGC ACGTACTGTG GCCGGGTATG GCCGGTACAG TGGCAAGATG 
TATTTCCAAA CCATGACTGA AGCTGAGTAC CAGACCAGCA TCCAGGAGAA GCTGCCACTC 
GTCATTGGCT CCTCCGCGGC AGGGCTGGTG TTCCTCATCG CTGTAGTCGT CATTGGGATT 
GTGTGCAACA GACGGGGCTT TGAGCGTGCT GACTCCGAAT ACACTGACAA GCTGCAGCAC 
TATACCAGTG GCCACAGTAC GTACCGCGGC CCCCCGCCAG GCCTGGGGGT CCGCTCTCTC 
TTCGTGACTC CAGGGATGAA GATTTATATT GATCCGTTCA CCTACGAAGA TCCCAATGAA 
GCTGTCAGGG AATTTGCTAA AGAAATTGAT ATCTCTTGTG TCAAAATCGA GCAGGTGATC 
GGGGCAGGGG AGTTTGGTGA GGTGTGCAGC GGGCATCTCA AGCTTCCTGG CAAAAGAGAG 
ATCTTTGTGG CCATCAAGAC TCTGAAGTCT GGTTACACAG AGAAGCAGAG ACGGGACTTC 
TTGAGTGAAG CCAGCATCAT GGGGCAGTTT GATCACCCCA ATGTCATCCA CCTGGAGGGG 
GTGGTGACCA AGAGTTCACC AGTCATGATC ATTACTGAGT TCATGGAGAA TGGCTCACTA 
GACTCCTTCT TGCGGCAAAA CGACGGGCAG TTCACAGTGA TCCAGCTGGT GGGCATGTTG 
CGCGGCATCG CAGCAGGCAT GAAGTACCTG GCTGATATGA ACTACGTGCA CCGGGACCTG 
GCTGCCCGCA ACATCCTGGT GAACAGCAAC CTGGTCTGCA AAGTGTCTGA CTTTGGCCTC 
TCCCGTTTTC TGGAGGACGA CACCTCTGAT CCCACCTACA CCAGTGCACT GGGTGGGAAG 
ATCCCAATAC GGTGGACAGC ACCTGAGGCA ATTCAGTACC GAAAATTCAC TTCGGCCAGT 
GATGTGTGGA GCTATGGAAT AGTCATGTGG GAGGTTATGT CGTATGGCGA GCGGCCTTAC 
TGGGATATGA CCAATCAAGA TGTGATAAAT GCTATTGAGC AGGACTATCG GTTGCCACCC 
CCTATGGATT GTCCAAATGC CTTGCACCAG CTAATGCTTG ACTGTTGGCA GAAGGATCGA 
AACCATAGAC CCAAATTTGG GCAAATTGTA AACACCTTGG ATAAAATGAT CCGAAATCCT 
AACAGCCTGA AAGCCATGGC ACCTCTTTCC TCTGGGATGA ACCTCCCCCT ACTTGACCGC 
ACAATCCCAG ATTATACCAG CTTCAACACT GTGGATGAAT GGCTGGATGC CATCAAGATG 
AGCCAGTACA AGGAGAGCTT TGCCAGTGCT GGCTTTACCA CCTTTGACGT AGTATCTCAG 
ATGACAGTAG AGGACATTCT GCGAGTTGGG GTCACTTTAG CAGGACATCA GAAGAAAATT 
CTGAACAGTA TCCAGGTGAT GAGAGCACAG ATGAACCAAA TTCAGTCTGT GGAGGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_014802421.1
CDS7..2946
Translation

Target ORF information:

RefSeq Version XM_014946935.1
Organism Calidris pugnax(ruff)
Definition Calidris pugnax EPH receptor B2 (EPHB2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014946935.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGACTCCA CAACGGCCAC GGCAGAGCTG GGCTGGACGG TACATCCTCC TTCGGGGTGG 
GAAGAGGTGA GTGGGTACGA CGAGAACATG AACACCATTC GCACCTACCA GGTGTGCAAC 
GTCTTTGAAT CCAGCCAAAA CAACTGGCTC CGGACCAAGT ACATCCGGAG GCGAGGAGCA 
CACCGCATCC ACGTGGAGAT GAAATTTTCT GTCCGGGACT GCAGCAGCAT CCCTAATGTA 
CCAGGCTCCT GTAAGGAGAC TTTTAACCTC TATTATTTCG AGTCAGACTT TGACTCGGCC 
ACCAAGACTT TTCCTAACTG GATGGAGAAT CCTTGGATGA AGGTGGACAC GATTGCTGCC 
GATGAGAGCT TCTCACAGGT GGACCTAGGT GGACGGGTGA TGAAGATTAA CACTGAGGTG 
CGCAGTTTTG GGCCTGTGTC CAAGAATGGT TTCTACCTGG CCTTCCAGGA CTACGGGGGC 
TGCATGTCTT TGATTGCTGT CCGTGTCTTT TACCGCAAGT GTCCCCGCGT GATCCAGAAT 
GGGGCCGTCT TCCAAGAAAC CCTCTCGGGA GCAGAGAGCA CCTCTCTGGT GGCCGCCCGG 
GGGACGTGCA TCACCAACGC GGAGGAGGTG GACGTGCCCA TCAAGCTGTA CTGCAACGGG 
GATGGCGAGT GGTTGGTGCC CATTGGCCGC TGCATGTGCA GGGCGGGCTA CGAGTCGGTG 
GAAAATGGGA CCGTCTGCAG AGGCTGCCCG TCGGGGACGT TCAAGGCCAG CCAAGGGGAT 
GAAGGATGCG TCCACTGCCC AATTAACAGC CGGACAACTT CAGAAGGGGC CACTAACTGC 
GTGTGCCGAA ACGGATATTA CCGGGCAGAT GCCGATCCTG TCGACATGCC ATGCACCACC 
ATCCCGTCTG CCCCCCAGGC CGTAATCTCC AGTGTGAATG AGACCTCGCT GATGCTGGAG 
TGGACCCCAC CGCGGGACTC AGGGGGCCGG GAGGACCTGG TGTACAACAT CATCTGCAAG 
AGCTGCGGCT CTGGCCGCGG GGCGTGCACA CGCTGTGGGG ACAACGTGCA GTTTGCCCCC 
CGCCAGCTGG GCTTGACAGA GCCCCGCATC TACATCAGCG ACCTGTTGGC CCACACCCAG 
TACACCTTTG AGATCCAGGC GGTGAATGGT GTCACCGACC AGAGCCCCTT CTCCCCGCAG 
TTTGCATCAG TGAACATCAC CACCAACCAG GCTGCTCCTT CAGCTGTGTC CATCATGCAC 
CAGGTCAGCC GCACCGTGGA CAGCATTACC CTCTCGTGGT CTCAGCCTGA CCAACCCAAC 
GGAGTCATCC TGGATTACGA GCTGCAATAT TATGAGAAGG ATCTGAGTGA GTTAAATTCA 
ACAACAGTGA AGAGCCCCAC CAACACTGTG GCAGTGCAAA ACCTCAAAGC TGGCACCATC 
TACGTCTTCC AGGTGCGGGC ACGTACTGTG GCCGGGTATG GCCGGTACAG TGGCAAGATG 
TATTTCCAAA CCATGACTGA AGCTGAGTAC CAGACCAGCA TCCAGGAGAA GCTGCCACTC 
GTCATTGGCT CCTCCGCGGC AGGGCTGGTG TTCCTCATCG CTGTAGTCGT CATTGGGATT 
GTGTGCAACA GACGGGGCTT TGAGCGTGCT GACTCCGAAT ACACTGACAA GCTGCAGCAC 
TATACCAGTG GCCACAGTAC GTACCGCGGC CCCCCGCCAG GCCTGGGGGT CCGCTCTCTC 
TTCGTGACTC CAGGGATGAA GATTTATATT GATCCGTTCA CCTACGAAGA TCCCAATGAA 
GCTGTCAGGG AATTTGCTAA AGAAATTGAT ATCTCTTGTG TCAAAATCGA GCAGGTGATC 
GGGGCAGGGG AGTTTGGTGA GGTGTGCAGC GGGCATCTCA AGCTTCCTGG CAAAAGAGAG 
ATCTTTGTGG CCATCAAGAC TCTGAAGTCT GGTTACACAG AGAAGCAGAG ACGGGACTTC 
TTGAGTGAAG CCAGCATCAT GGGGCAGTTT GATCACCCCA ATGTCATCCA CCTGGAGGGG 
GTGGTGACCA AGAGTTCACC AGTCATGATC ATTACTGAGT TCATGGAGAA TGGCTCACTA 
GACTCCTTCT TGCGGCAAAA CGACGGGCAG TTCACAGTGA TCCAGCTGGT GGGCATGTTG 
CGCGGCATCG CAGCAGGCAT GAAGTACCTG GCTGATATGA ACTACGTGCA CCGGGACCTG 
GCTGCCCGCA ACATCCTGGT GAACAGCAAC CTGGTCTGCA AAGTGTCTGA CTTTGGCCTC 
TCCCGTTTTC TGGAGGACGA CACCTCTGAT CCCACCTACA CCAGTGCACT GGGTGGGAAG 
ATCCCAATAC GGTGGACAGC ACCTGAGGCA ATTCAGTACC GAAAATTCAC TTCGGCCAGT 
GATGTGTGGA GCTATGGAAT AGTCATGTGG GAGGTTATGT CGTATGGCGA GCGGCCTTAC 
TGGGATATGA CCAATCAAGA TGTGATAAAT GCTATTGAGC AGGACTATCG GTTGCCACCC 
CCTATGGATT GTCCAAATGC CTTGCACCAG CTAATGCTTG ACTGTTGGCA GAAGGATCGA 
AACCATAGAC CCAAATTTGG GCAAATTGTA AACACCTTGG ATAAAATGAT CCGAAATCCT 
AACAGCCTGA AAGCCATGGC ACCTCTTTCC TCTGGGATGA ACCTCCCCCT ACTTGACCGC 
ACAATCCCAG ATTATACCAG CTTCAACACT GTGGATGAAT GGCTGGATGC CATCAAGATG 
AGCCAGTACA AGGAGAGCTT TGCCAGTGCT GGCTTTACCA CCTTTGACGT AGTATCTCAG 
ATGACAGTAG AGGACATTCT GCGAGTTGGG GTCACTTTAG CAGGACATCA GAAGAAAATT 
CTGAACAGTA TCCAGGTGAT GAGAGCACAG ATGAACCAAA TTCAGTCTGT GGAGGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCa68665
Clone ID Related Accession (Same CDS sequence) XM_014946936.1
Accession Version XM_014946936.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-12-08
Organism Calidris pugnax(ruff)
Product ephrin type-B receptor 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGACCACTG ACTCCTTGTT TCACCTTCCT CCTGCAGAGA CGCTGATGGA CTCCACAACG 
GCCACGGCAG AGCTGGGCTG GACGGTACAT CCTCCTTCGG GGTGGGAAGA GGTGAGTGGG 
TACGACGAGA ACATGAACAC CATTCGCACC TACCAGGTGT GCAACGTCTT TGAATCCAGC 
CAAAACAACT GGCTCCGGAC CAAGTACATC CGGAGGCGAG GAGCACACCG CATCCACGTG 
GAGATGAAAT TTTCTGTCCG GGACTGCAGC AGCATCCCTA ATGTACCAGG CTCCTGTAAG 
GAGACTTTTA ACCTCTATTA TTTCGAGTCA GACTTTGACT CGGCCACCAA GACTTTTCCT 
AACTGGATGG AGAATCCTTG GATGAAGGTG GACACGATTG CTGCCGATGA GAGCTTCTCA 
CAGGTGGACC TAGGTGGACG GGTGATGAAG ATTAACACTG AGGTGCGCAG TTTTGGGCCT 
GTGTCCAAGA ATGGTTTCTA CCTGGCCTTC CAGGACTACG GGGGCTGCAT GTCTTTGATT 
GCTGTCCGTG TCTTTTACCG CAAGTGTCCC CGCGTGATCC AGAATGGGGC CGTCTTCCAA 
GAAACCCTCT CGGGAGCAGA GAGCACCTCT CTGGTGGCCG CCCGGGGGAC GTGCATCACC 
AACGCGGAGG AGGTGGACGT GCCCATCAAG CTGTACTGCA ACGGGGATGG CGAGTGGTTG 
GTGCCCATTG GCCGCTGCAT GTGCAGGGCG GGCTACGAGT CGGTGGAAAA TGGGACCGTC 
TGCAGAGGCT GCCCGTCGGG GACGTTCAAG GCCAGCCAAG GGGATGAAGG ATGCGTCCAC 
TGCCCAATTA ACAGCCGGAC AACTTCAGAA GGGGCCACTA ACTGCGTGTG CCGAAACGGA 
TATTACCGGG CAGATGCCGA TCCTGTCGAC ATGCCATGCA CCACCATCCC GTCTGCCCCC 
CAGGCCGTAA TCTCCAGTGT GAATGAGACC TCGCTGATGC TGGAGTGGAC CCCACCGCGG 
GACTCAGGGG GCCGGGAGGA CCTGGTGTAC AACATCATCT GCAAGAGCTG CGGCTCTGGC 
CGCGGGGCGT GCACACGCTG TGGGGACAAC GTGCAGTTTG CCCCCCGCCA GCTGGGCTTG 
ACAGAGCCCC GCATCTACAT CAGCGACCTG TTGGCCCACA CCCAGTACAC CTTTGAGATC 
CAGGCGGTGA ATGGTGTCAC CGACCAGAGC CCCTTCTCCC CGCAGTTTGC ATCAGTGAAC 
ATCACCACCA ACCAGGCTGC TCCTTCAGCT GTGTCCATCA TGCACCAGGT CAGCCGCACC 
GTGGACAGCA TTACCCTCTC GTGGTCTCAG CCTGACCAAC CCAACGGAGT CATCCTGGAT 
TACGAGCTGC AATATTATGA GAAGGATCTG AGTGAGTTAA ATTCAACAAC AGTGAAGAGC 
CCCACCAACA CTGTGGCAGT GCAAAACCTC AAAGCTGGCA CCATCTACGT CTTCCAGGTG 
CGGGCACGTA CTGTGGCCGG GTATGGCCGG TACAGTGGCA AGATGTATTT CCAAACCATG 
ACTGAAGCTG AGTACCAGAC CAGCATCCAG GAGAAGCTGC CACTCGTCAT TGGCTCCTCC 
GCGGCAGGGC TGGTGTTCCT CATCGCTGTA GTCGTCATTG GGATTGTGTG CAACAGAAGA 
CGGGGCTTTG AGCGTGCTGA CTCCGAATAC ACTGACAAGC TGCAGCACTA TACCAGTGGC 
CACATGACTC CAGGGATGAA GATTTATATT GATCCGTTCA CCTACGAAGA TCCCAATGAA 
GCTGTCAGGG AATTTGCTAA AGAAATTGAT ATCTCTTGTG TCAAAATCGA GCAGGTGATC 
GGGGCAGGGG AGTTTGGTGA GGTGTGCAGC GGGCATCTCA AGCTTCCTGG CAAAAGAGAG 
ATCTTTGTGG CCATCAAGAC TCTGAAGTCT GGTTACACAG AGAAGCAGAG ACGGGACTTC 
TTGAGTGAAG CCAGCATCAT GGGGCAGTTT GATCACCCCA ATGTCATCCA CCTGGAGGGG 
GTGGTGACCA AGAGTTCACC AGTCATGATC ATTACTGAGT TCATGGAGAA TGGCTCACTA 
GACTCCTTCT TGCGGCAAAA CGACGGGCAG TTCACAGTGA TCCAGCTGGT GGGCATGTTG 
CGCGGCATCG CAGCAGGCAT GAAGTACCTG GCTGATATGA ACTACGTGCA CCGGGACCTG 
GCTGCCCGCA ACATCCTGGT GAACAGCAAC CTGGTCTGCA AAGTGTCTGA CTTTGGCCTC 
TCCCGTTTTC TGGAGGACGA CACCTCTGAT CCCACCTACA CCAGTGCACT GGGTGGGAAG 
ATCCCAATAC GGTGGACAGC ACCTGAGGCA ATTCAGTACC GAAAATTCAC TTCGGCCAGT 
GATGTGTGGA GCTATGGAAT AGTCATGTGG GAGGTTATGT CGTATGGCGA GCGGCCTTAC 
TGGGATATGA CCAATCAAGA TGTGATAAAT GCTATTGAGC AGGACTATCG GTTGCCACCC 
CCTATGGATT GTCCAAATGC CTTGCACCAG CTAATGCTTG ACTGTTGGCA GAAGGATCGA 
AACCATAGAC CCAAATTTGG GCAAATTGTA AACACCTTGG ATAAAATGAT CCGAAATCCT 
AACAGCCTGA AAGCCATGGC ACCTCTTTCC TCTGGGATGA ACCTCCCCCT ACTTGACCGC 
ACAATCCCAG ATTATACCAG CTTCAACACT GTGGATGAAT GGCTGGATGC CATCAAGATG 
AGCCAGTACA AGGAGAGCTT TGCCAGTGCT GGCTTTACCA CCTTTGACGT AGTATCTCAG 
ATGACAGTAG AGGACATTCT GCGAGTTGGG GTCACTTTAG CAGGACATCA GAAGAAAATT 
CTGAACAGTA TCCAGGTGAT GAGAGCACAG ATGAACCAAA TTCAGTCTGT GGAGGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_014802422.1
CDS38..2977
Translation

Target ORF information:

RefSeq Version XM_014946936.1
Organism Calidris pugnax(ruff)
Definition Calidris pugnax EPH receptor B2 (EPHB2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014946936.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGACCACTG ACTCCTTGTT TCACCTTCCT CCTGCAGAGA CGCTGATGGA CTCCACAACG 
GCCACGGCAG AGCTGGGCTG GACGGTACAT CCTCCTTCGG GGTGGGAAGA GGTGAGTGGG 
TACGACGAGA ACATGAACAC CATTCGCACC TACCAGGTGT GCAACGTCTT TGAATCCAGC 
CAAAACAACT GGCTCCGGAC CAAGTACATC CGGAGGCGAG GAGCACACCG CATCCACGTG 
GAGATGAAAT TTTCTGTCCG GGACTGCAGC AGCATCCCTA ATGTACCAGG CTCCTGTAAG 
GAGACTTTTA ACCTCTATTA TTTCGAGTCA GACTTTGACT CGGCCACCAA GACTTTTCCT 
AACTGGATGG AGAATCCTTG GATGAAGGTG GACACGATTG CTGCCGATGA GAGCTTCTCA 
CAGGTGGACC TAGGTGGACG GGTGATGAAG ATTAACACTG AGGTGCGCAG TTTTGGGCCT 
GTGTCCAAGA ATGGTTTCTA CCTGGCCTTC CAGGACTACG GGGGCTGCAT GTCTTTGATT 
GCTGTCCGTG TCTTTTACCG CAAGTGTCCC CGCGTGATCC AGAATGGGGC CGTCTTCCAA 
GAAACCCTCT CGGGAGCAGA GAGCACCTCT CTGGTGGCCG CCCGGGGGAC GTGCATCACC 
AACGCGGAGG AGGTGGACGT GCCCATCAAG CTGTACTGCA ACGGGGATGG CGAGTGGTTG 
GTGCCCATTG GCCGCTGCAT GTGCAGGGCG GGCTACGAGT CGGTGGAAAA TGGGACCGTC 
TGCAGAGGCT GCCCGTCGGG GACGTTCAAG GCCAGCCAAG GGGATGAAGG ATGCGTCCAC 
TGCCCAATTA ACAGCCGGAC AACTTCAGAA GGGGCCACTA ACTGCGTGTG CCGAAACGGA 
TATTACCGGG CAGATGCCGA TCCTGTCGAC ATGCCATGCA CCACCATCCC GTCTGCCCCC 
CAGGCCGTAA TCTCCAGTGT GAATGAGACC TCGCTGATGC TGGAGTGGAC CCCACCGCGG 
GACTCAGGGG GCCGGGAGGA CCTGGTGTAC AACATCATCT GCAAGAGCTG CGGCTCTGGC 
CGCGGGGCGT GCACACGCTG TGGGGACAAC GTGCAGTTTG CCCCCCGCCA GCTGGGCTTG 
ACAGAGCCCC GCATCTACAT CAGCGACCTG TTGGCCCACA CCCAGTACAC CTTTGAGATC 
CAGGCGGTGA ATGGTGTCAC CGACCAGAGC CCCTTCTCCC CGCAGTTTGC ATCAGTGAAC 
ATCACCACCA ACCAGGCTGC TCCTTCAGCT GTGTCCATCA TGCACCAGGT CAGCCGCACC 
GTGGACAGCA TTACCCTCTC GTGGTCTCAG CCTGACCAAC CCAACGGAGT CATCCTGGAT 
TACGAGCTGC AATATTATGA GAAGGATCTG AGTGAGTTAA ATTCAACAAC AGTGAAGAGC 
CCCACCAACA CTGTGGCAGT GCAAAACCTC AAAGCTGGCA CCATCTACGT CTTCCAGGTG 
CGGGCACGTA CTGTGGCCGG GTATGGCCGG TACAGTGGCA AGATGTATTT CCAAACCATG 
ACTGAAGCTG AGTACCAGAC CAGCATCCAG GAGAAGCTGC CACTCGTCAT TGGCTCCTCC 
GCGGCAGGGC TGGTGTTCCT CATCGCTGTA GTCGTCATTG GGATTGTGTG CAACAGAAGA 
CGGGGCTTTG AGCGTGCTGA CTCCGAATAC ACTGACAAGC TGCAGCACTA TACCAGTGGC 
CACATGACTC CAGGGATGAA GATTTATATT GATCCGTTCA CCTACGAAGA TCCCAATGAA 
GCTGTCAGGG AATTTGCTAA AGAAATTGAT ATCTCTTGTG TCAAAATCGA GCAGGTGATC 
GGGGCAGGGG AGTTTGGTGA GGTGTGCAGC GGGCATCTCA AGCTTCCTGG CAAAAGAGAG 
ATCTTTGTGG CCATCAAGAC TCTGAAGTCT GGTTACACAG AGAAGCAGAG ACGGGACTTC 
TTGAGTGAAG CCAGCATCAT GGGGCAGTTT GATCACCCCA ATGTCATCCA CCTGGAGGGG 
GTGGTGACCA AGAGTTCACC AGTCATGATC ATTACTGAGT TCATGGAGAA TGGCTCACTA 
GACTCCTTCT TGCGGCAAAA CGACGGGCAG TTCACAGTGA TCCAGCTGGT GGGCATGTTG 
CGCGGCATCG CAGCAGGCAT GAAGTACCTG GCTGATATGA ACTACGTGCA CCGGGACCTG 
GCTGCCCGCA ACATCCTGGT GAACAGCAAC CTGGTCTGCA AAGTGTCTGA CTTTGGCCTC 
TCCCGTTTTC TGGAGGACGA CACCTCTGAT CCCACCTACA CCAGTGCACT GGGTGGGAAG 
ATCCCAATAC GGTGGACAGC ACCTGAGGCA ATTCAGTACC GAAAATTCAC TTCGGCCAGT 
GATGTGTGGA GCTATGGAAT AGTCATGTGG GAGGTTATGT CGTATGGCGA GCGGCCTTAC 
TGGGATATGA CCAATCAAGA TGTGATAAAT GCTATTGAGC AGGACTATCG GTTGCCACCC 
CCTATGGATT GTCCAAATGC CTTGCACCAG CTAATGCTTG ACTGTTGGCA GAAGGATCGA 
AACCATAGAC CCAAATTTGG GCAAATTGTA AACACCTTGG ATAAAATGAT CCGAAATCCT 
AACAGCCTGA AAGCCATGGC ACCTCTTTCC TCTGGGATGA ACCTCCCCCT ACTTGACCGC 
ACAATCCCAG ATTATACCAG CTTCAACACT GTGGATGAAT GGCTGGATGC CATCAAGATG 
AGCCAGTACA AGGAGAGCTT TGCCAGTGCT GGCTTTACCA CCTTTGACGT AGTATCTCAG 
ATGACAGTAG AGGACATTCT GCGAGTTGGG GTCACTTTAG CAGGACATCA GAAGAAAATT 
CTGAACAGTA TCCAGGTGAT GAGAGCACAG ATGAACCAAA TTCAGTCTGT GGAGGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCa68666
Clone ID Related Accession (Same CDS sequence) XM_014946937.1
Accession Version XM_014946937.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-12-08
Organism Calidris pugnax(ruff)
Product ephrin type-B receptor 2 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGACCACTG ACTCCTTGTT TCACCTTCCT CCTGCAGAGA CGCTGATGGA CTCCACAACG 
GCCACGGCAG AGCTGGGCTG GACGGTACAT CCTCCTTCGG GGTGGGAAGA GGTGAGTGGG 
TACGACGAGA ACATGAACAC CATTCGCACC TACCAGGTGT GCAACGTCTT TGAATCCAGC 
CAAAACAACT GGCTCCGGAC CAAGTACATC CGGAGGCGAG GAGCACACCG CATCCACGTG 
GAGATGAAAT TTTCTGTCCG GGACTGCAGC AGCATCCCTA ATGTACCAGG CTCCTGTAAG 
GAGACTTTTA ACCTCTATTA TTTCGAGTCA GACTTTGACT CGGCCACCAA GACTTTTCCT 
AACTGGATGG AGAATCCTTG GATGAAGGTG GACACGATTG CTGCCGATGA GAGCTTCTCA 
CAGGTGGACC TAGGTGGACG GGTGATGAAG ATTAACACTG AGGTGCGCAG TTTTGGGCCT 
GTGTCCAAGA ATGGTTTCTA CCTGGCCTTC CAGGACTACG GGGGCTGCAT GTCTTTGATT 
GCTGTCCGTG TCTTTTACCG CAAGTGTCCC CGCGTGATCC AGAATGGGGC CGTCTTCCAA 
GAAACCCTCT CGGGAGCAGA GAGCACCTCT CTGGTGGCCG CCCGGGGGAC GTGCATCACC 
AACGCGGAGG AGGTGGACGT GCCCATCAAG CTGTACTGCA ACGGGGATGG CGAGTGGTTG 
GTGCCCATTG GCCGCTGCAT GTGCAGGGCG GGCTACGAGT CGGTGGAAAA TGGGACCGTC 
TGCAGAGGCT GCCCGTCGGG GACGTTCAAG GCCAGCCAAG GGGATGAAGG ATGCGTCCAC 
TGCCCAATTA ACAGCCGGAC AACTTCAGAA GGGGCCACTA ACTGCGTGTG CCGAAACGGA 
TATTACCGGG CAGATGCCGA TCCTGTCGAC ATGCCATGCA CCACCATCCC GTCTGCCCCC 
CAGGCCGTAA TCTCCAGTGT GAATGAGACC TCGCTGATGC TGGAGTGGAC CCCACCGCGG 
GACTCAGGGG GCCGGGAGGA CCTGGTGTAC AACATCATCT GCAAGAGCTG CGGCTCTGGC 
CGCGGGGCGT GCACACGCTG TGGGGACAAC GTGCAGTTTG CCCCCCGCCA GCTGGGCTTG 
ACAGAGCCCC GCATCTACAT CAGCGACCTG TTGGCCCACA CCCAGTACAC CTTTGAGATC 
CAGGCGGTGA ATGGTGTCAC CGACCAGAGC CCCTTCTCCC CGCAGTTTGC ATCAGTGAAC 
ATCACCACCA ACCAGGCTGC TCCTTCAGCT GTGTCCATCA TGCACCAGGT CAGCCGCACC 
GTGGACAGCA TTACCCTCTC GTGGTCTCAG CCTGACCAAC CCAACGGAGT CATCCTGGAT 
TACGAGCTGC AATATTATGA GAAGGATCTG AGTGAGTTAA ATTCAACAAC AGTGAAGAGC 
CCCACCAACA CTGTGGCAGT GCAAAACCTC AAAGCTGGCA CCATCTACGT CTTCCAGGTG 
CGGGCACGTA CTGTGGCCGG GTATGGCCGG TACAGTGGCA AGATGTATTT CCAAACCATG 
ACTGAAGCTG AGTACCAGAC CAGCATCCAG GAGAAGCTGC CACTCGTCAT TGGCTCCTCC 
GCGGCAGGGC TGGTGTTCCT CATCGCTGTA GTCGTCATTG GGATTGTGTG CAACAGACGG 
GGCTTTGAGC GTGCTGACTC CGAATACACT GACAAGCTGC AGCACTATAC CAGTGGCCAC 
ATGACTCCAG GGATGAAGAT TTATATTGAT CCGTTCACCT ACGAAGATCC CAATGAAGCT 
GTCAGGGAAT TTGCTAAAGA AATTGATATC TCTTGTGTCA AAATCGAGCA GGTGATCGGG 
GCAGGGGAGT TTGGTGAGGT GTGCAGCGGG CATCTCAAGC TTCCTGGCAA AAGAGAGATC 
TTTGTGGCCA TCAAGACTCT GAAGTCTGGT TACACAGAGA AGCAGAGACG GGACTTCTTG 
AGTGAAGCCA GCATCATGGG GCAGTTTGAT CACCCCAATG TCATCCACCT GGAGGGGGTG 
GTGACCAAGA GTTCACCAGT CATGATCATT ACTGAGTTCA TGGAGAATGG CTCACTAGAC 
TCCTTCTTGC GGCAAAACGA CGGGCAGTTC ACAGTGATCC AGCTGGTGGG CATGTTGCGC 
GGCATCGCAG CAGGCATGAA GTACCTGGCT GATATGAACT ACGTGCACCG GGACCTGGCT 
GCCCGCAACA TCCTGGTGAA CAGCAACCTG GTCTGCAAAG TGTCTGACTT TGGCCTCTCC 
CGTTTTCTGG AGGACGACAC CTCTGATCCC ACCTACACCA GTGCACTGGG TGGGAAGATC 
CCAATACGGT GGACAGCACC TGAGGCAATT CAGTACCGAA AATTCACTTC GGCCAGTGAT 
GTGTGGAGCT ATGGAATAGT CATGTGGGAG GTTATGTCGT ATGGCGAGCG GCCTTACTGG 
GATATGACCA ATCAAGATGT GATAAATGCT ATTGAGCAGG ACTATCGGTT GCCACCCCCT 
ATGGATTGTC CAAATGCCTT GCACCAGCTA ATGCTTGACT GTTGGCAGAA GGATCGAAAC 
CATAGACCCA AATTTGGGCA AATTGTAAAC ACCTTGGATA AAATGATCCG AAATCCTAAC 
AGCCTGAAAG CCATGGCACC TCTTTCCTCT GGGATGAACC TCCCCCTACT TGACCGCACA 
ATCCCAGATT ATACCAGCTT CAACACTGTG GATGAATGGC TGGATGCCAT CAAGATGAGC 
CAGTACAAGG AGAGCTTTGC CAGTGCTGGC TTTACCACCT TTGACGTAGT ATCTCAGATG 
ACAGTAGAGG ACATTCTGCG AGTTGGGGTC ACTTTAGCAG GACATCAGAA GAAAATTCTG 
AACAGTATCC AGGTGATGAG AGCACAGATG AACCAAATTC AGTCTGTGGA GGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_014802423.1
CDS38..2974
Translation

Target ORF information:

RefSeq Version XM_014946937.1
Organism Calidris pugnax(ruff)
Definition Calidris pugnax EPH receptor B2 (EPHB2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014946937.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGACCACTG ACTCCTTGTT TCACCTTCCT CCTGCAGAGA CGCTGATGGA CTCCACAACG 
GCCACGGCAG AGCTGGGCTG GACGGTACAT CCTCCTTCGG GGTGGGAAGA GGTGAGTGGG 
TACGACGAGA ACATGAACAC CATTCGCACC TACCAGGTGT GCAACGTCTT TGAATCCAGC 
CAAAACAACT GGCTCCGGAC CAAGTACATC CGGAGGCGAG GAGCACACCG CATCCACGTG 
GAGATGAAAT TTTCTGTCCG GGACTGCAGC AGCATCCCTA ATGTACCAGG CTCCTGTAAG 
GAGACTTTTA ACCTCTATTA TTTCGAGTCA GACTTTGACT CGGCCACCAA GACTTTTCCT 
AACTGGATGG AGAATCCTTG GATGAAGGTG GACACGATTG CTGCCGATGA GAGCTTCTCA 
CAGGTGGACC TAGGTGGACG GGTGATGAAG ATTAACACTG AGGTGCGCAG TTTTGGGCCT 
GTGTCCAAGA ATGGTTTCTA CCTGGCCTTC CAGGACTACG GGGGCTGCAT GTCTTTGATT 
GCTGTCCGTG TCTTTTACCG CAAGTGTCCC CGCGTGATCC AGAATGGGGC CGTCTTCCAA 
GAAACCCTCT CGGGAGCAGA GAGCACCTCT CTGGTGGCCG CCCGGGGGAC GTGCATCACC 
AACGCGGAGG AGGTGGACGT GCCCATCAAG CTGTACTGCA ACGGGGATGG CGAGTGGTTG 
GTGCCCATTG GCCGCTGCAT GTGCAGGGCG GGCTACGAGT CGGTGGAAAA TGGGACCGTC 
TGCAGAGGCT GCCCGTCGGG GACGTTCAAG GCCAGCCAAG GGGATGAAGG ATGCGTCCAC 
TGCCCAATTA ACAGCCGGAC AACTTCAGAA GGGGCCACTA ACTGCGTGTG CCGAAACGGA 
TATTACCGGG CAGATGCCGA TCCTGTCGAC ATGCCATGCA CCACCATCCC GTCTGCCCCC 
CAGGCCGTAA TCTCCAGTGT GAATGAGACC TCGCTGATGC TGGAGTGGAC CCCACCGCGG 
GACTCAGGGG GCCGGGAGGA CCTGGTGTAC AACATCATCT GCAAGAGCTG CGGCTCTGGC 
CGCGGGGCGT GCACACGCTG TGGGGACAAC GTGCAGTTTG CCCCCCGCCA GCTGGGCTTG 
ACAGAGCCCC GCATCTACAT CAGCGACCTG TTGGCCCACA CCCAGTACAC CTTTGAGATC 
CAGGCGGTGA ATGGTGTCAC CGACCAGAGC CCCTTCTCCC CGCAGTTTGC ATCAGTGAAC 
ATCACCACCA ACCAGGCTGC TCCTTCAGCT GTGTCCATCA TGCACCAGGT CAGCCGCACC 
GTGGACAGCA TTACCCTCTC GTGGTCTCAG CCTGACCAAC CCAACGGAGT CATCCTGGAT 
TACGAGCTGC AATATTATGA GAAGGATCTG AGTGAGTTAA ATTCAACAAC AGTGAAGAGC 
CCCACCAACA CTGTGGCAGT GCAAAACCTC AAAGCTGGCA CCATCTACGT CTTCCAGGTG 
CGGGCACGTA CTGTGGCCGG GTATGGCCGG TACAGTGGCA AGATGTATTT CCAAACCATG 
ACTGAAGCTG AGTACCAGAC CAGCATCCAG GAGAAGCTGC CACTCGTCAT TGGCTCCTCC 
GCGGCAGGGC TGGTGTTCCT CATCGCTGTA GTCGTCATTG GGATTGTGTG CAACAGACGG 
GGCTTTGAGC GTGCTGACTC CGAATACACT GACAAGCTGC AGCACTATAC CAGTGGCCAC 
ATGACTCCAG GGATGAAGAT TTATATTGAT CCGTTCACCT ACGAAGATCC CAATGAAGCT 
GTCAGGGAAT TTGCTAAAGA AATTGATATC TCTTGTGTCA AAATCGAGCA GGTGATCGGG 
GCAGGGGAGT TTGGTGAGGT GTGCAGCGGG CATCTCAAGC TTCCTGGCAA AAGAGAGATC 
TTTGTGGCCA TCAAGACTCT GAAGTCTGGT TACACAGAGA AGCAGAGACG GGACTTCTTG 
AGTGAAGCCA GCATCATGGG GCAGTTTGAT CACCCCAATG TCATCCACCT GGAGGGGGTG 
GTGACCAAGA GTTCACCAGT CATGATCATT ACTGAGTTCA TGGAGAATGG CTCACTAGAC 
TCCTTCTTGC GGCAAAACGA CGGGCAGTTC ACAGTGATCC AGCTGGTGGG CATGTTGCGC 
GGCATCGCAG CAGGCATGAA GTACCTGGCT GATATGAACT ACGTGCACCG GGACCTGGCT 
GCCCGCAACA TCCTGGTGAA CAGCAACCTG GTCTGCAAAG TGTCTGACTT TGGCCTCTCC 
CGTTTTCTGG AGGACGACAC CTCTGATCCC ACCTACACCA GTGCACTGGG TGGGAAGATC 
CCAATACGGT GGACAGCACC TGAGGCAATT CAGTACCGAA AATTCACTTC GGCCAGTGAT 
GTGTGGAGCT ATGGAATAGT CATGTGGGAG GTTATGTCGT ATGGCGAGCG GCCTTACTGG 
GATATGACCA ATCAAGATGT GATAAATGCT ATTGAGCAGG ACTATCGGTT GCCACCCCCT 
ATGGATTGTC CAAATGCCTT GCACCAGCTA ATGCTTGACT GTTGGCAGAA GGATCGAAAC 
CATAGACCCA AATTTGGGCA AATTGTAAAC ACCTTGGATA AAATGATCCG AAATCCTAAC 
AGCCTGAAAG CCATGGCACC TCTTTCCTCT GGGATGAACC TCCCCCTACT TGACCGCACA 
ATCCCAGATT ATACCAGCTT CAACACTGTG GATGAATGGC TGGATGCCAT CAAGATGAGC 
CAGTACAAGG AGAGCTTTGC CAGTGCTGGC TTTACCACCT TTGACGTAGT ATCTCAGATG 
ACAGTAGAGG ACATTCTGCG AGTTGGGGTC ACTTTAGCAG GACATCAGAA GAAAATTCTG 
AACAGTATCC AGGTGATGAG AGCACAGATG AACCAAATTC AGTCTGTGGA GGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCa68667
Clone ID Related Accession (Same CDS sequence) XM_014946938.1
Accession Version XM_014946938.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-12-08
Organism Calidris pugnax(ruff)
Product ephrin type-B receptor 2 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGACCACTG ACTCCTTGTT TCACCTTCCT CCTGCAGAGA CGCTGATGGA CTCCACAACG 
GCCACGGCAG AGCTGGGCTG GACGGTACAT CCTCCTTCGG GGTGGGAAGA GGTGAGTGGG 
TACGACGAGA ACATGAACAC CATTCGCACC TACCAGGTGT GCAACGTCTT TGAATCCAGC 
CAAAACAACT GGCTCCGGAC CAAGTACATC CGGAGGCGAG GAGCACACCG CATCCACGTG 
GAGATGAAAT TTTCTGTCCG GGACTGCAGC AGCATCCCTA ATGTACCAGG CTCCTGTAAG 
GAGACTTTTA ACCTCTATTA TTTCGAGTCA GACTTTGACT CGGCCACCAA GACTTTTCCT 
AACTGGATGG AGAATCCTTG GATGAAGGTG GACACGATTG CTGCCGATGA GAGCTTCTCA 
CAGGTGGACC TAGGTGGACG GGTGATGAAG ATTAACACTG AGGTGCGCAG TTTTGGGCCT 
GTGTCCAAGA ATGGTTTCTA CCTGGCCTTC CAGGACTACG GGGGCTGCAT GTCTTTGATT 
GCTGTCCGTG TCTTTTACCG CAAGTGTCCC CGCGTGATCC AGAATGGGGC CGTCTTCCAA 
GAAACCCTCT CGGGAGCAGA GAGCACCTCT CTGGTGGCCG CCCGGGGGAC GTGCATCACC 
AACGCGGAGG AGGTGGACGT GCCCATCAAG CTGTACTGCA ACGGGGATGG CGAGTGGTTG 
GTGCCCATTG GCCGCTGCAT GTGCAGGGCG GGCTACGAGT CGGTGGAAAA TGGGACCGTC 
TGCAGAGGCT GCCCGTCGGG GACGTTCAAG GCCAGCCAAG GGGATGAAGG ATGCGTCCAC 
TGCCCAATTA ACAGCCGGAC AACTTCAGAA GGGGCCACTA ACTGCGTGTG CCGAAACGGA 
TATTACCGGG CAGATGCCGA TCCTGTCGAC ATGCCATGCA CCACCATCCC GTCTGCCCCC 
CAGGCCGTAA TCTCCAGTGT GAATGAGACC TCGCTGATGC TGGAGTGGAC CCCACCGCGG 
GACTCAGGGG GCCGGGAGGA CCTGGTGTAC AACATCATCT GCAAGAGCTG CGGCTCTGGC 
CGCGGGGCGT GCACACGCTG TGGGGACAAC GTGCAGTTTG CCCCCCGCCA GCTGGGCTTG 
ACAGAGCCCC GCATCTACAT CAGCGACCTG TTGGCCCACA CCCAGTACAC CTTTGAGATC 
CAGGCGGTGA ATGGTGTCAC CGACCAGAGC CCCTTCTCCC CGCAGTTTGC ATCAGTGAAC 
ATCACCACCA ACCAGGCTGC TCCTTCAGCT GTGTCCATCA TGCACCAGGT CAGCCGCACC 
GTGGACAGCA TTACCCTCTC GTGGTCTCAG CCTGACCAAC CCAACGGAGT CATCCTGGAT 
TACGAGCTGC AATATTATGA GAAGGATCTG AGTGAGTTAA ATTCAACAAC AGTGAAGAGC 
CCCACCAACA CTGTGGCAGT GCAAAACCTC AAAGCTGGCA CCATCTACGT CTTCCAGGTG 
CGGGCACGTA CTGTGGCCGG GTATGGCCGG TACAGTGGCA AGATGTATTT CCAAACCATG 
ACTGAAGCTG AGTACCAGAC CAGCATCCAG GAGAAGCTGC CACTCGTCAT TGGCTCCTCC 
GCGGCAGGGC TGGTGTTCCT CATCGCTGTA GTCGTCATTG GGATTGTGTG CAACAGAAGA 
CGGGGCTTTG AGCGTGCTGA CTCCGAATAC ACTGACAAGC TGCAGCACTA TACCAGTGGC 
CACAGTACGT ACCGCGGCCC CCCGCCAGGC CTGGGGGTCC GCTCTCTCTT CGTGACTCCA 
GGGATGAAGA TTTATATTGA TCCGTTCACC TACGAAGATC CCAATGAAGC TGTCAGGGAA 
TTTGCTAAAG AAATTGATAT CTCTTGTGTC AAAATCGAGC AGGTGATCGG GGCAGGGGAG 
TTTGGTGAGG TGTGCAGCGG GCATCTCAAG CTTCCTGGCA AAAGAGAGAT CTTTGTGGCC 
ATCAAGACTC TGAAGTCTGG TTACACAGAG AAGCAGAGAC GGGACTTCTT GAGTGAAGCC 
AGCATCATGG GGCAGTTTGA TCACCCCAAT GTCATCCACC TGGAGGGGGT GGTGACCAAG 
AGTTCACCAG TCATGATCAT TACTGAGTTC ATGGAGAATG GCTCACTAGA CTCCTTCTTG 
CGGCAAAACG ACGGGCAGTT CACAGTGATC CAGCTGGTGG GCATGTTGCG CGGCATCGCA 
GCAGGCATGA AGTACCTGGC TGATATGAAC TACGTGCACC GGGACCTGGC TGCCCGCAAC 
ATCCTGGTGA ACAGCAACCT GGTCTGCAAA GTGTCTGACT TTGGCCTCTC CCGTTTTCTG 
GAGGACGACA CCTCTGATCC CACCTACACC AGTGCACTGG GTGGGAAGAT CCCAATACGG 
TGGACAGCAC CTGAGGCAAT TCAGTACCGA AAATTCACTT CGGCCAGTGA TGTGTGGAGC 
TATGGAATAG TCATGTGGGA GGTTATGTCG TATGGCGAGC GGCCTTACTG GGATATGACC 
AATCAAGATG TGATAAATGC TATTGAGCAG GACTATCGGT TGCCACCCCC TATGGATTGT 
CCAAATGCCT TGCACCAGCT AATGCTTGAC TGTTGGCAGA AGGATCGAAA CCATAGACCC 
AAATTTGGGC AAATTGTAAA CACCTTGGAT AAAATGATCC GAAATCCTAA CAGCCTGAAA 
GCCATGGCAC CTCTTTCCTC TGGGATGAAC CTCCCCCTAC TTGACCGCAC AATCCCAGAT 
TATACCAGCT TCAACACTGT GGATGAATGG CTGGATGCCA TCAAGATGAG CCAGTACAAG 
GAGAGCTTTG CCAGTGCTGG CTTTACCACC TTTGACGTAG TATCTCAGAT GACAGTAGAG 
GACATTCTGC GAGTTGGGGT CACTTTAGCA GGACATCAGA AGAAAATTCT GAACAGTATC 
CAGGTGATGA GAGCACAGAT GAACCAAATT CAGTCTGTGG AGGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_014802424.1
CDS38..3025
Translation

Target ORF information:

RefSeq Version XM_014946938.1
Organism Calidris pugnax(ruff)
Definition Calidris pugnax EPH receptor B2 (EPHB2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014946938.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGACCACTG ACTCCTTGTT TCACCTTCCT CCTGCAGAGA CGCTGATGGA CTCCACAACG 
GCCACGGCAG AGCTGGGCTG GACGGTACAT CCTCCTTCGG GGTGGGAAGA GGTGAGTGGG 
TACGACGAGA ACATGAACAC CATTCGCACC TACCAGGTGT GCAACGTCTT TGAATCCAGC 
CAAAACAACT GGCTCCGGAC CAAGTACATC CGGAGGCGAG GAGCACACCG CATCCACGTG 
GAGATGAAAT TTTCTGTCCG GGACTGCAGC AGCATCCCTA ATGTACCAGG CTCCTGTAAG 
GAGACTTTTA ACCTCTATTA TTTCGAGTCA GACTTTGACT CGGCCACCAA GACTTTTCCT 
AACTGGATGG AGAATCCTTG GATGAAGGTG GACACGATTG CTGCCGATGA GAGCTTCTCA 
CAGGTGGACC TAGGTGGACG GGTGATGAAG ATTAACACTG AGGTGCGCAG TTTTGGGCCT 
GTGTCCAAGA ATGGTTTCTA CCTGGCCTTC CAGGACTACG GGGGCTGCAT GTCTTTGATT 
GCTGTCCGTG TCTTTTACCG CAAGTGTCCC CGCGTGATCC AGAATGGGGC CGTCTTCCAA 
GAAACCCTCT CGGGAGCAGA GAGCACCTCT CTGGTGGCCG CCCGGGGGAC GTGCATCACC 
AACGCGGAGG AGGTGGACGT GCCCATCAAG CTGTACTGCA ACGGGGATGG CGAGTGGTTG 
GTGCCCATTG GCCGCTGCAT GTGCAGGGCG GGCTACGAGT CGGTGGAAAA TGGGACCGTC 
TGCAGAGGCT GCCCGTCGGG GACGTTCAAG GCCAGCCAAG GGGATGAAGG ATGCGTCCAC 
TGCCCAATTA ACAGCCGGAC AACTTCAGAA GGGGCCACTA ACTGCGTGTG CCGAAACGGA 
TATTACCGGG CAGATGCCGA TCCTGTCGAC ATGCCATGCA CCACCATCCC GTCTGCCCCC 
CAGGCCGTAA TCTCCAGTGT GAATGAGACC TCGCTGATGC TGGAGTGGAC CCCACCGCGG 
GACTCAGGGG GCCGGGAGGA CCTGGTGTAC AACATCATCT GCAAGAGCTG CGGCTCTGGC 
CGCGGGGCGT GCACACGCTG TGGGGACAAC GTGCAGTTTG CCCCCCGCCA GCTGGGCTTG 
ACAGAGCCCC GCATCTACAT CAGCGACCTG TTGGCCCACA CCCAGTACAC CTTTGAGATC 
CAGGCGGTGA ATGGTGTCAC CGACCAGAGC CCCTTCTCCC CGCAGTTTGC ATCAGTGAAC 
ATCACCACCA ACCAGGCTGC TCCTTCAGCT GTGTCCATCA TGCACCAGGT CAGCCGCACC 
GTGGACAGCA TTACCCTCTC GTGGTCTCAG CCTGACCAAC CCAACGGAGT CATCCTGGAT 
TACGAGCTGC AATATTATGA GAAGGATCTG AGTGAGTTAA ATTCAACAAC AGTGAAGAGC 
CCCACCAACA CTGTGGCAGT GCAAAACCTC AAAGCTGGCA CCATCTACGT CTTCCAGGTG 
CGGGCACGTA CTGTGGCCGG GTATGGCCGG TACAGTGGCA AGATGTATTT CCAAACCATG 
ACTGAAGCTG AGTACCAGAC CAGCATCCAG GAGAAGCTGC CACTCGTCAT TGGCTCCTCC 
GCGGCAGGGC TGGTGTTCCT CATCGCTGTA GTCGTCATTG GGATTGTGTG CAACAGAAGA 
CGGGGCTTTG AGCGTGCTGA CTCCGAATAC ACTGACAAGC TGCAGCACTA TACCAGTGGC 
CACAGTACGT ACCGCGGCCC CCCGCCAGGC CTGGGGGTCC GCTCTCTCTT CGTGACTCCA 
GGGATGAAGA TTTATATTGA TCCGTTCACC TACGAAGATC CCAATGAAGC TGTCAGGGAA 
TTTGCTAAAG AAATTGATAT CTCTTGTGTC AAAATCGAGC AGGTGATCGG GGCAGGGGAG 
TTTGGTGAGG TGTGCAGCGG GCATCTCAAG CTTCCTGGCA AAAGAGAGAT CTTTGTGGCC 
ATCAAGACTC TGAAGTCTGG TTACACAGAG AAGCAGAGAC GGGACTTCTT GAGTGAAGCC 
AGCATCATGG GGCAGTTTGA TCACCCCAAT GTCATCCACC TGGAGGGGGT GGTGACCAAG 
AGTTCACCAG TCATGATCAT TACTGAGTTC ATGGAGAATG GCTCACTAGA CTCCTTCTTG 
CGGCAAAACG ACGGGCAGTT CACAGTGATC CAGCTGGTGG GCATGTTGCG CGGCATCGCA 
GCAGGCATGA AGTACCTGGC TGATATGAAC TACGTGCACC GGGACCTGGC TGCCCGCAAC 
ATCCTGGTGA ACAGCAACCT GGTCTGCAAA GTGTCTGACT TTGGCCTCTC CCGTTTTCTG 
GAGGACGACA CCTCTGATCC CACCTACACC AGTGCACTGG GTGGGAAGAT CCCAATACGG 
TGGACAGCAC CTGAGGCAAT TCAGTACCGA AAATTCACTT CGGCCAGTGA TGTGTGGAGC 
TATGGAATAG TCATGTGGGA GGTTATGTCG TATGGCGAGC GGCCTTACTG GGATATGACC 
AATCAAGATG TGATAAATGC TATTGAGCAG GACTATCGGT TGCCACCCCC TATGGATTGT 
CCAAATGCCT TGCACCAGCT AATGCTTGAC TGTTGGCAGA AGGATCGAAA CCATAGACCC 
AAATTTGGGC AAATTGTAAA CACCTTGGAT AAAATGATCC GAAATCCTAA CAGCCTGAAA 
GCCATGGCAC CTCTTTCCTC TGGGATGAAC CTCCCCCTAC TTGACCGCAC AATCCCAGAT 
TATACCAGCT TCAACACTGT GGATGAATGG CTGGATGCCA TCAAGATGAG CCAGTACAAG 
GAGAGCTTTG CCAGTGCTGG CTTTACCACC TTTGACGTAG TATCTCAGAT GACAGTAGAG 
GACATTCTGC GAGTTGGGGT CACTTTAGCA GGACATCAGA AGAAAATTCT GAACAGTATC 
CAGGTGATGA GAGCACAGAT GAACCAAATT CAGTCTGTGG AGGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed