MYH15 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH15
Entrez Gene ID	106891017
Full Name	myosin heavy chain 15
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014947183.1	XP_014802669.1	myosin-15

Pathways from BioSystems
Gene Ontology

The following MYH15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa68850	XM_014947183.1 Latest version!	Calidris pugnax myosin, heavy chain 15 (MYH15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH15 ( XM_014947183.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014802669.1 Calidris pugnax myosin, heavy chain 15 (MYH15), mRNA.

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CloneID	OCa68850
Clone ID Related Accession (Same CDS sequence)	XM_014947183.1
Accession Version	XM_014947183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	myosin-15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACA TGTCGGAATT CGGGGAGGCT GCTCCCTACC TCCGAAAGAG CGAAAAGGAG 
CTGATGATGG CACAGACCGT TGCCTTTGAT GGGAAGAAGA AATGTTGGGT TCCCGATGAC 
AAGCAAGCCT ACGTTGAAGC CGAAATTACA GAAACCAGCG GTGGCAAAGT GACCGTGGAG 
ACGACAGACG GGAGGACCAT GACTATAAAA GAAGACGACG TGCAGTCGAT GAACCCTCCC 
AAATTTGACA TGATCGAGGA CATGGCTATG CTGACCCATC TGAACGAGGC GTCTGTGTTG 
TACAACCTGA GAAAGCGCTA CAAGAACTGG ATGATCTATA CCTATTCCGG TTTATTCTGC 
GTGACTATAA ATCCCTACAA GTGGTTGCCT GTCTACAAGT CGGAGGTGGT TGCTGCATAC 
AAAGGCAAGA GGCGCTCGGA GGCTCCTCCC CACATCTTCT CCATTGCTGA TAACGCGTAC 
CACGACATGC TGCGTAATCG GGAGAATCAG TCTATGCTGA TCACTGGAGA ATCCGGTGCT 
GGCAAGACTG TCAACACGAA ACGTGTCATC CAGTACTTTG CCACAGTGGC AGCCTTGGGT 
GAGCCTGGTA AAAAGAACCA ACCAGCTACT AAAACTGGGG GAACCTTGGA GGATCAAATC 
ATTCAAGCAA ACCCAGCCCT AGAAGCTTTT GGAAATGCCA AAACCCTGAG AAATGACAAC 
TCCTCCCGTT TTGGTAAATT TATCCGAATC CATTTTGGAA CTACAGGCAA GCTGTCATCT 
GCTGATATTG AGATTTATTT ACTGGAGAAA TCCCGAGTGA TTTTCCAGCA ACCAGGTGAA 
AGAGACTATC ACATCTTCTA CCAAATCCTG TCAGGAAAAA AGCCAGAGTT ACTGGATATG 
CTCCTGGTAT CTACCAACCC ATATGACTAC CACTTTTGCT CCCAAGGAGT AGTTACAGTG 
GACAACTTGG ATGATGGAGA AGAATTGTTG GCAACTGATC AAGCCATGGA CATCTTGGGA 
TTTGTGCCTG ATGAGAAGTC TGGTTCCTAC AAGCTCACAG GTGCCATCAT GCACTTTGGG 
AACATGAAAT TCAAACAAAG ACCCAGAGAA GAGCAGGCAG AGGCTGATGG CACTGAAAGT 
GCTGACAAAG CTGCCTACCT AATGGGAATC AACTCTTCTG ATTTGATTAA GGGTTTATTG 
CACCCTAGAG TGAAGGTTGG CAACGAGTAT GTGACCAAAG GTCAAAGTGT TGAACAGGTT 
TTGTATGCTG TTGGAGCCTT ATCTAAGGCA GTGTATGATC GGATGTTCAA GTGGCTGGTG 
GTCCGTATCA ACAAGACCTT GGACACTAAG CTGCCAAGAC AGTTCTTCAT TGGAGTCCTG 
GATATTGCCG GGTTTGAAAT CTTTGATTTT AACAGCTTTG AGCAACTCTG CATCAATTAC 
ACTAATGAGA AACTGCAACA GTTTTTCAAT CATCACATGT TTGTCCTGGA ACAAGAAGAG 
TATAAGAAGG AAGGCATTGA TTGGGTATTC ATTGATTTTG GCATGGACTT GCAAGCCTGC 
ATTGATTTAA TTGAGAAGCC ATTGGGAATC CTGTCTATCC TTGAAGAGGA ATGTATGTTC 
CCAAAAGCTA CAGATATGAC ATTCAAAGCC AAACTCTACG ACAATCATCT TGGCAAGTCA 
CCTAACTTAC AGAAGCCCAG GCCTGATAAG AAAAGGAAAT ATGAAGCTCA CTTTGAACTT 
CTTCATTATG CTGGTGCAGT TCCCTATAAC ATCATTGGGT GGCTTGAGAA GAACAAAGAC 
CCACTTAATG AAACTGTAGT AGGTATTTTC CAAAAGTCAT CCAACAAACT CCTGGCAAGC 
TTGTTTGAAA GCTACGTTGG CGCTGACAGC GCTGACCAGG GTGGAGAAAA GAAACGCAAG 
AAAGGTGCTT CTTTTCAAAC AGTGTCCTCA TTGCACAAGG AAAATTTAAA TAAGCTGATG 
ACTAACCTAA AATCTACAGC CCCTCACTTT GTACGATGCA TTATCCCCAA TGAATCAAAA 
ACTCCAGGTG AAATGGATGC TTTCCTTGTC TTGCATCAGC TCCGCTGTAA TGGTGTCCTG 
GAAGGCATCC GCATTTGCCG TAAGGGTTTC CCAAACAGGG TGCTTTACGC TGACTTTAAA 
CAAAGGTACC GCATTCTGAA TCCAGGCGCA ATCCCGGAGG ATACATTTGT GGATAGCAGA 
AAAGCTGCTG AAAAACTGCT GGCATCTTTA GATGTTGACC ATAACCAATA TCGTTTTGGA 
CACACCAAGG TGTTCTTCAA GGCTGGTCTC TTGGGCCATC TAGAAGAAAT GAGGGATGAG 
AGACTTGCGA AAATCTTAAC GATGATCCAG GCAAGAGCAC GTGGAAGACT GATGCGGATT 
GAGTTTCAGA AGATAGTGGA GCGCAGGGAT GCCCTTCTTG TAATTCAGTG GAACATCCGT 
GCTTTTATGG CTGTGAAGAA CTGGCCCTGG ATGAAGCTTT TCTTTAAGAT CAAACCTCTT 
CTGAAGTCTG CAGAAACTGA AAAGGAGATG GCCAATATGA AGGAAGAGTT CTTGAAACTG 
AAGGAGGCCC TGGAAAAATC TGAAGCCAGG AGAAAGGAAC TTGAAGAAAA ACAAGTCTCT 
CTAGTTCAGG AAAAAAATGA TTTGCTTCTC CAGCTCCAAG CTGAGCAAGA CACTCTGGCA 
GATGCTGAGG AACGATGCGA CTTGTTGATT AAATCCAAGA TTCAGCTGGA GGCCAAAGTC 
AAGGAACTGA CAGAGCGGGT TGAGGACGAG GAGGAGATGA ATTCTGAGCT TACTTCTAAG 
AAGAGAAAAT TGGAAGATGA GTGCTCTGAA CTCAAGAAGG ATATTGATGA TCTTGAAATT 
ACACTTGCAA AAGTAGAGAA AGAGAAGCAT GCTACAGAAA ACAAGGTCAA AAATCTGACA 
GAAGAAATGG CGACTCTTGA TGAAAATATC AGCAAACTTA CTAAGGAGAA AAAGTCCTTG 
CAGGAAGCTC ATCAACAAGT TCTGGATGAC CTGCAAGCAG AGGAGGACAA GGTCAACACA 
CTGAGCAAAG CTAAAGTGAA ACTGGAACAG CAAGTGGATG ATCTTGAGGG CTCACTTGAA 
CAAGAGAAGA AAGTGAGGAT GGACCTGGAA AGGGCAAAAC GCAAACTGGA AGGGGATTTG 
AAGTTGACTC AAGAGAGTGT CATGGACTTG GAGAACGATA AGCTGCAGAT GGAAGAAAAG 
CTAAAAAAGA AAGAATTTGA AATGAGCCAG CTGAATTCCA AGATAGAAGA TGAACAAGCT 
ATAGTGATGC AGCTGCAGAA GAAAATCAAG GAGCTTCAGG CTCGTATAGA AGAACTAGAA 
GAGGAGTTGG AAGCAGAAAG AGCTGCTCGA GCCAAGGTGG AAAAGCAGAG ATCCGACTTG 
TCCCGAGAGC TGGAGGAATT AAGCGAGCGA CTTGAGGAGG CAGGGGGAGC CACAGCTGCC 
CAGCTGGAGA TGAACAAGAA ACGTGAGGCG GAGTTCCTGA AGCTGCGGAG GGACCTTGAG 
GAGGCCACAC TGCACTACGA AGCCACAGCC GCCACCCTGA GGAAGAAGCA TGCAGACAGT 
GTGGCTGAGA TGGGGGAGCA ACTGGACAAC CTGCAGCGGG TCAAGCAGAA ACTGGAAAAG 
GAGAAAAGTG AGCTGAAGAT GGAAGTCGAT GATCTGTCAT CCAACATGGA GCAGATGGTT 
AAGGGAAAGG CAAATGCAGA GAAACTTTGC CGCACTTATG AAGACCACCT GAATGAGACA 
AAAACTAAAC TGGAGGAAAT GACTCGCCTC ATGAATGACC TCACTACTCA GAAGACAAAA 
CTCCAGAGTG AGAATGGTGA ATTTGTGAGA CAACTCGAAG AGAAGGAGTC ACTGATAAGT 
CAGCTGTCCC GGGGGAAAAC ATCATTTACA CAACAGATTG AAGAACTTAG GAGACAGCTA 
GAAGAGGAAA CCAAGTCCAA AAATGCCCTG GCTCATGCCC TGCAAGCGGC CAGGCACGAC 
TGTGATCTCT TGCGGGAGCA GTATGAGGAG GAGCAAGAAG CCAAGGCAGA GCTGCAGCGG 
GCTCTCTCCA AGGGAAATGC AGAAGTGGCA CAGTGGAGAA CAAAGTATGA GACTGACGCC 
ATTCAGAGAA CTGAGGAGCT GGAAGATGCC AAGAAGAAGC TTGCCGCTCG CCTGCAAGAA 
GCTGAGGAAG CAATCGAGGC GGCCAATGCC AAGTGCTCTT CTCTGGAAAA GACCAAGCAC 
AGGCTGCAGA ATGAGCTGGA AGACATGATG ATTGACCTAG AGAAGGCCAA CTCAGCAGCT 
GCCTCCCTGG ACAAGAAGCA GCGTGGGTTT GACAAGATCA TCAATGACTG GAAGCAGAAG 
TACGAAGAGT CACAGGCTGA GCTGGAGGCT TCCCAGAAGG AGGCCCGTGG CCTCAGCACT 
GAGCTCTTCA AGCTGAAGAA TGCCTATGAG GAGACACTGG ACCATCTGGA GACGTTGAAA 
CGGGAAAACA AGAACCTCCA AGAGGAGATT TCTGATCTGA CCAATCAGAT TAGTGAAGGA 
AACAAGAGCC TCCATGAAAT AGAAAAAGTC AAGAAACAGG TTGAACAAGA GAAGTCAGAA 
GTTCAGCTGG CTCTGGAAGA AGCAGAGGGA GCTTTGGAGC ATGAAGAAAG TAAGACCCTT 
CGCTTTCAGC TTGAACTTTC TCAGCTTAAA GCAGAGTTTG AAAGGAAACT GACAGAAAAG 
GAGGAAGAAA TGGAAAATAT AAGGAGAAAT CAGCAACGTG CCATAGATTC ACTGCAGTCC 
ACCCTTGATT CTGAAGCACG GAGCAGAAAT GAGGCCATCC GGCTGAAGAA GAAGATGGAG 
GGGGACCTCA ACGAGATGGA AATCCAGCTC AGCCATGCCA ACAGGCATGC TGCAGAAGCA 
ACAAAGTCAA CACGTGTCTT GCAGACACAA ATAAAGGAGC TTCAGGTGCA GCTGGATGAC 
TCAGGACATG TTAATGAAGA TCTGAAGGAG CAGCTGGCGG TCTCTGACAG GAGGAACAAT 
CTTCTCCAGT CGGAGCTGGA TGAGCTGAGG GCTTTGCTGG ACCAGACTGA AAGGGCGAGG 
AAGCTGGCAG AGCATGAGCT GCTGGAAGCC ACCGAACGTG TGAACTTGCT TCACACTCAG 
AACACAAGCC TGATCAATCA AAAGAAGAAG TTGGAGGGTG ACATTTCCCA GATGCAGAAT 
GAAGTGGAGG AATCAATCCA GGAGTGCCGG AACGCAGAGG AAAAAGCCAA GAAAGCCATC 
ACAGATGCAG CAATGATGGC CGAGGAGCTT AAAAAGGAGC AAGATACTAG TGCTCACTTG 
GAGAGAATGA AGAAGAATAT GGAGCAAACC ATTAAAGATC TCCAAAAGCG ACTGGATGAA 
GCAGAACAAA TAGCCCTGAA AGGCGGCAAG AAGCAGATCC AGAAACTGGA ATCCAGGGTT 
CGTGAGCTGG AGAATGAACT TGAGACTGAA CTCCGTCGCA ATTCAGATGC CCAAAAAGGA 
GCCCGCAAGT TTGAGAGACG CATCAAAGAG CTGACTTACC AGTCAGAGGA AGATAAGAAG 
AATCTGGCCA GGATGCAGGA TCTGATTGAC AAGCTACAGT TAAAAGTGAA GAGCTACAAG 
CACCAGGCAG AAGAAGCTGA AGCTCAAGCC AACCTGTACC TTTCGAAGTA CAGAAAACAG 
CAACATGATC TGGATGATGC TGAAGAACGG GCTGAAATAG CTGAATCTCA AGTTAATAAG 
CTGAGGAGCA AGTCAAGAGA TATTGGCATG AAAAAGGTTC ATGAAGAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014802669.1
CDS	191..6004
Translation	MMDMSEFGEAAPYLRKSEKELMMAQTVAFDGKKKCWVPDDKQAYVEAEITETSGGKVTVETTDGRTMTIKEDDVQSMNPPKFDMIEDMAMLTHLNEASVLYNLRKRYKNWMIYTYSGLFCVTINPYKWLPVYKSEVVAAYKGKRRSEAPPHIFSIADNAYHDMLRNRENQSMLITGESGAGKTVNTKRVIQYFATVAALGEPGKKNQPATKTGGTLEDQIIQANPALEAFGNAKTLRNDNSSRFGKFIRIHFGTTGKLSSADIEIYLLEKSRVIFQQPGERDYHIFYQILSGKKPELLDMLLVSTNPYDYHFCSQGVVTVDNLDDGEELLATDQAMDILGFVPDEKSGSYKLTGAIMHFGNMKFKQRPREEQAEADGTESADKAAYLMGINSSDLIKGLLHPRVKVGNEYVTKGQSVEQVLYAVGALSKAVYDRMFKWLVVRINKTLDTKLPRQFFIGVLDIAGFEIFDFNSFEQLCINYTNEKLQQFFNHHMFVLEQEEYKKEGIDWVFIDFGMDLQACIDLIEKPLGILSILEEECMFPKATDMTFKAKLYDNHLGKSPNLQKPRPDKKRKYEAHFELLHYAGAVPYNIIGWLEKNKDPLNETVVGIFQKSSNKLLASLFESYVGADSADQGGEKKRKKGASFQTVSSLHKENLNKLMTNLKSTAPHFVRCIIPNESKTPGEMDAFLVLHQLRCNGVLEGIRICRKGFPNRVLYADFKQRYRILNPGAIPEDTFVDSRKAAEKLLASLDVDHNQYRFGHTKVFFKAGLLGHLEEMRDERLAKILTMIQARARGRLMRIEFQKIVERRDALLVIQWNIRAFMAVKNWPWMKLFFKIKPLLKSAETEKEMANMKEEFLKLKEALEKSEARRKELEEKQVSLVQEKNDLLLQLQAEQDTLADAEERCDLLIKSKIQLEAKVKELTERVEDEEEMNSELTSKKRKLEDECSELKKDIDDLEITLAKVEKEKHATENKVKNLTEEMATLDENISKLTKEKKSLQEAHQQVLDDLQAEEDKVNTLSKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESVMDLENDKLQMEEKLKKKEFEMSQLNSKIEDEQAIVMQLQKKIKELQARIEELEEELEAERAARAKVEKQRSDLSRELEELSERLEEAGGATAAQLEMNKKREAEFLKLRRDLEEATLHYEATAATLRKKHADSVAEMGEQLDNLQRVKQKLEKEKSELKMEVDDLSSNMEQMVKGKANAEKLCRTYEDHLNETKTKLEEMTRLMNDLTTQKTKLQSENGEFVRQLEEKESLISQLSRGKTSFTQQIEELRRQLEEETKSKNALAHALQAARHDCDLLREQYEEEQEAKAELQRALSKGNAEVAQWRTKYETDAIQRTEELEDAKKKLAARLQEAEEAIEAANAKCSSLEKTKHRLQNELEDMMIDLEKANSAAASLDKKQRGFDKIINDWKQKYEESQAELEASQKEARGLSTELFKLKNAYEETLDHLETLKRENKNLQEEISDLTNQISEGNKSLHEIEKVKKQVEQEKSEVQLALEEAEGALEHEESKTLRFQLELSQLKAEFERKLTEKEEEMENIRRNQQRAIDSLQSTLDSEARSRNEAIRLKKKMEGDLNEMEIQLSHANRHAAEATKSTRVLQTQIKELQVQLDDSGHVNEDLKEQLAVSDRRNNLLQSELDELRALLDQTERARKLAEHELLEATERVNLLHTQNTSLINQKKKLEGDISQMQNEVEESIQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQKRLDEAEQIALKGGKKQIQKLESRVRELENELETELRRNSDAQKGARKFERRIKELTYQSEEDKKNLARMQDLIDKLQLKVKSYKHQAEEAEAQANLYLSKYRKQQHDLDDAEERAEIAESQVNKLRSKSRDIGMKKVHEEE

Target ORF information:

RefSeq Version	XM_014947183.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax myosin, heavy chain 15 (MYH15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014947183.1

ORF Insert Sequence:

ATGATGGACA TGTCGGAATT CGGGGAGGCT GCTCCCTACC TCCGAAAGAG CGAAAAGGAG 
CTGATGATGG CACAGACCGT TGCCTTTGAT GGGAAGAAGA AATGTTGGGT TCCCGATGAC 
AAGCAAGCCT ACGTTGAAGC CGAAATTACA GAAACCAGCG GTGGCAAAGT GACCGTGGAG 
ACGACAGACG GGAGGACCAT GACTATAAAA GAAGACGACG TGCAGTCGAT GAACCCTCCC 
AAATTTGACA TGATCGAGGA CATGGCTATG CTGACCCATC TGAACGAGGC GTCTGTGTTG 
TACAACCTGA GAAAGCGCTA CAAGAACTGG ATGATCTATA CCTATTCCGG TTTATTCTGC 
GTGACTATAA ATCCCTACAA GTGGTTGCCT GTCTACAAGT CGGAGGTGGT TGCTGCATAC 
AAAGGCAAGA GGCGCTCGGA GGCTCCTCCC CACATCTTCT CCATTGCTGA TAACGCGTAC 
CACGACATGC TGCGTAATCG GGAGAATCAG TCTATGCTGA TCACTGGAGA ATCCGGTGCT 
GGCAAGACTG TCAACACGAA ACGTGTCATC CAGTACTTTG CCACAGTGGC AGCCTTGGGT 
GAGCCTGGTA AAAAGAACCA ACCAGCTACT AAAACTGGGG GAACCTTGGA GGATCAAATC 
ATTCAAGCAA ACCCAGCCCT AGAAGCTTTT GGAAATGCCA AAACCCTGAG AAATGACAAC 
TCCTCCCGTT TTGGTAAATT TATCCGAATC CATTTTGGAA CTACAGGCAA GCTGTCATCT 
GCTGATATTG AGATTTATTT ACTGGAGAAA TCCCGAGTGA TTTTCCAGCA ACCAGGTGAA 
AGAGACTATC ACATCTTCTA CCAAATCCTG TCAGGAAAAA AGCCAGAGTT ACTGGATATG 
CTCCTGGTAT CTACCAACCC ATATGACTAC CACTTTTGCT CCCAAGGAGT AGTTACAGTG 
GACAACTTGG ATGATGGAGA AGAATTGTTG GCAACTGATC AAGCCATGGA CATCTTGGGA 
TTTGTGCCTG ATGAGAAGTC TGGTTCCTAC AAGCTCACAG GTGCCATCAT GCACTTTGGG 
AACATGAAAT TCAAACAAAG ACCCAGAGAA GAGCAGGCAG AGGCTGATGG CACTGAAAGT 
GCTGACAAAG CTGCCTACCT AATGGGAATC AACTCTTCTG ATTTGATTAA GGGTTTATTG 
CACCCTAGAG TGAAGGTTGG CAACGAGTAT GTGACCAAAG GTCAAAGTGT TGAACAGGTT 
TTGTATGCTG TTGGAGCCTT ATCTAAGGCA GTGTATGATC GGATGTTCAA GTGGCTGGTG 
GTCCGTATCA ACAAGACCTT GGACACTAAG CTGCCAAGAC AGTTCTTCAT TGGAGTCCTG 
GATATTGCCG GGTTTGAAAT CTTTGATTTT AACAGCTTTG AGCAACTCTG CATCAATTAC 
ACTAATGAGA AACTGCAACA GTTTTTCAAT CATCACATGT TTGTCCTGGA ACAAGAAGAG 
TATAAGAAGG AAGGCATTGA TTGGGTATTC ATTGATTTTG GCATGGACTT GCAAGCCTGC 
ATTGATTTAA TTGAGAAGCC ATTGGGAATC CTGTCTATCC TTGAAGAGGA ATGTATGTTC 
CCAAAAGCTA CAGATATGAC ATTCAAAGCC AAACTCTACG ACAATCATCT TGGCAAGTCA 
CCTAACTTAC AGAAGCCCAG GCCTGATAAG AAAAGGAAAT ATGAAGCTCA CTTTGAACTT 
CTTCATTATG CTGGTGCAGT TCCCTATAAC ATCATTGGGT GGCTTGAGAA GAACAAAGAC 
CCACTTAATG AAACTGTAGT AGGTATTTTC CAAAAGTCAT CCAACAAACT CCTGGCAAGC 
TTGTTTGAAA GCTACGTTGG CGCTGACAGC GCTGACCAGG GTGGAGAAAA GAAACGCAAG 
AAAGGTGCTT CTTTTCAAAC AGTGTCCTCA TTGCACAAGG AAAATTTAAA TAAGCTGATG 
ACTAACCTAA AATCTACAGC CCCTCACTTT GTACGATGCA TTATCCCCAA TGAATCAAAA 
ACTCCAGGTG AAATGGATGC TTTCCTTGTC TTGCATCAGC TCCGCTGTAA TGGTGTCCTG 
GAAGGCATCC GCATTTGCCG TAAGGGTTTC CCAAACAGGG TGCTTTACGC TGACTTTAAA 
CAAAGGTACC GCATTCTGAA TCCAGGCGCA ATCCCGGAGG ATACATTTGT GGATAGCAGA 
AAAGCTGCTG AAAAACTGCT GGCATCTTTA GATGTTGACC ATAACCAATA TCGTTTTGGA 
CACACCAAGG TGTTCTTCAA GGCTGGTCTC TTGGGCCATC TAGAAGAAAT GAGGGATGAG 
AGACTTGCGA AAATCTTAAC GATGATCCAG GCAAGAGCAC GTGGAAGACT GATGCGGATT 
GAGTTTCAGA AGATAGTGGA GCGCAGGGAT GCCCTTCTTG TAATTCAGTG GAACATCCGT 
GCTTTTATGG CTGTGAAGAA CTGGCCCTGG ATGAAGCTTT TCTTTAAGAT CAAACCTCTT 
CTGAAGTCTG CAGAAACTGA AAAGGAGATG GCCAATATGA AGGAAGAGTT CTTGAAACTG 
AAGGAGGCCC TGGAAAAATC TGAAGCCAGG AGAAAGGAAC TTGAAGAAAA ACAAGTCTCT 
CTAGTTCAGG AAAAAAATGA TTTGCTTCTC CAGCTCCAAG CTGAGCAAGA CACTCTGGCA 
GATGCTGAGG AACGATGCGA CTTGTTGATT AAATCCAAGA TTCAGCTGGA GGCCAAAGTC 
AAGGAACTGA CAGAGCGGGT TGAGGACGAG GAGGAGATGA ATTCTGAGCT TACTTCTAAG 
AAGAGAAAAT TGGAAGATGA GTGCTCTGAA CTCAAGAAGG ATATTGATGA TCTTGAAATT 
ACACTTGCAA AAGTAGAGAA AGAGAAGCAT GCTACAGAAA ACAAGGTCAA AAATCTGACA 
GAAGAAATGG CGACTCTTGA TGAAAATATC AGCAAACTTA CTAAGGAGAA AAAGTCCTTG 
CAGGAAGCTC ATCAACAAGT TCTGGATGAC CTGCAAGCAG AGGAGGACAA GGTCAACACA 
CTGAGCAAAG CTAAAGTGAA ACTGGAACAG CAAGTGGATG ATCTTGAGGG CTCACTTGAA 
CAAGAGAAGA AAGTGAGGAT GGACCTGGAA AGGGCAAAAC GCAAACTGGA AGGGGATTTG 
AAGTTGACTC AAGAGAGTGT CATGGACTTG GAGAACGATA AGCTGCAGAT GGAAGAAAAG 
CTAAAAAAGA AAGAATTTGA AATGAGCCAG CTGAATTCCA AGATAGAAGA TGAACAAGCT 
ATAGTGATGC AGCTGCAGAA GAAAATCAAG GAGCTTCAGG CTCGTATAGA AGAACTAGAA 
GAGGAGTTGG AAGCAGAAAG AGCTGCTCGA GCCAAGGTGG AAAAGCAGAG ATCCGACTTG 
TCCCGAGAGC TGGAGGAATT AAGCGAGCGA CTTGAGGAGG CAGGGGGAGC CACAGCTGCC 
CAGCTGGAGA TGAACAAGAA ACGTGAGGCG GAGTTCCTGA AGCTGCGGAG GGACCTTGAG 
GAGGCCACAC TGCACTACGA AGCCACAGCC GCCACCCTGA GGAAGAAGCA TGCAGACAGT 
GTGGCTGAGA TGGGGGAGCA ACTGGACAAC CTGCAGCGGG TCAAGCAGAA ACTGGAAAAG 
GAGAAAAGTG AGCTGAAGAT GGAAGTCGAT GATCTGTCAT CCAACATGGA GCAGATGGTT 
AAGGGAAAGG CAAATGCAGA GAAACTTTGC CGCACTTATG AAGACCACCT GAATGAGACA 
AAAACTAAAC TGGAGGAAAT GACTCGCCTC ATGAATGACC TCACTACTCA GAAGACAAAA 
CTCCAGAGTG AGAATGGTGA ATTTGTGAGA CAACTCGAAG AGAAGGAGTC ACTGATAAGT 
CAGCTGTCCC GGGGGAAAAC ATCATTTACA CAACAGATTG AAGAACTTAG GAGACAGCTA 
GAAGAGGAAA CCAAGTCCAA AAATGCCCTG GCTCATGCCC TGCAAGCGGC CAGGCACGAC 
TGTGATCTCT TGCGGGAGCA GTATGAGGAG GAGCAAGAAG CCAAGGCAGA GCTGCAGCGG 
GCTCTCTCCA AGGGAAATGC AGAAGTGGCA CAGTGGAGAA CAAAGTATGA GACTGACGCC 
ATTCAGAGAA CTGAGGAGCT GGAAGATGCC AAGAAGAAGC TTGCCGCTCG CCTGCAAGAA 
GCTGAGGAAG CAATCGAGGC GGCCAATGCC AAGTGCTCTT CTCTGGAAAA GACCAAGCAC 
AGGCTGCAGA ATGAGCTGGA AGACATGATG ATTGACCTAG AGAAGGCCAA CTCAGCAGCT 
GCCTCCCTGG ACAAGAAGCA GCGTGGGTTT GACAAGATCA TCAATGACTG GAAGCAGAAG 
TACGAAGAGT CACAGGCTGA GCTGGAGGCT TCCCAGAAGG AGGCCCGTGG CCTCAGCACT 
GAGCTCTTCA AGCTGAAGAA TGCCTATGAG GAGACACTGG ACCATCTGGA GACGTTGAAA 
CGGGAAAACA AGAACCTCCA AGAGGAGATT TCTGATCTGA CCAATCAGAT TAGTGAAGGA 
AACAAGAGCC TCCATGAAAT AGAAAAAGTC AAGAAACAGG TTGAACAAGA GAAGTCAGAA 
GTTCAGCTGG CTCTGGAAGA AGCAGAGGGA GCTTTGGAGC ATGAAGAAAG TAAGACCCTT 
CGCTTTCAGC TTGAACTTTC TCAGCTTAAA GCAGAGTTTG AAAGGAAACT GACAGAAAAG 
GAGGAAGAAA TGGAAAATAT AAGGAGAAAT CAGCAACGTG CCATAGATTC ACTGCAGTCC 
ACCCTTGATT CTGAAGCACG GAGCAGAAAT GAGGCCATCC GGCTGAAGAA GAAGATGGAG 
GGGGACCTCA ACGAGATGGA AATCCAGCTC AGCCATGCCA ACAGGCATGC TGCAGAAGCA 
ACAAAGTCAA CACGTGTCTT GCAGACACAA ATAAAGGAGC TTCAGGTGCA GCTGGATGAC 
TCAGGACATG TTAATGAAGA TCTGAAGGAG CAGCTGGCGG TCTCTGACAG GAGGAACAAT 
CTTCTCCAGT CGGAGCTGGA TGAGCTGAGG GCTTTGCTGG ACCAGACTGA AAGGGCGAGG 
AAGCTGGCAG AGCATGAGCT GCTGGAAGCC ACCGAACGTG TGAACTTGCT TCACACTCAG 
AACACAAGCC TGATCAATCA AAAGAAGAAG TTGGAGGGTG ACATTTCCCA GATGCAGAAT 
GAAGTGGAGG AATCAATCCA GGAGTGCCGG AACGCAGAGG AAAAAGCCAA GAAAGCCATC 
ACAGATGCAG CAATGATGGC CGAGGAGCTT AAAAAGGAGC AAGATACTAG TGCTCACTTG 
GAGAGAATGA AGAAGAATAT GGAGCAAACC ATTAAAGATC TCCAAAAGCG ACTGGATGAA 
GCAGAACAAA TAGCCCTGAA AGGCGGCAAG AAGCAGATCC AGAAACTGGA ATCCAGGGTT 
CGTGAGCTGG AGAATGAACT TGAGACTGAA CTCCGTCGCA ATTCAGATGC CCAAAAAGGA 
GCCCGCAAGT TTGAGAGACG CATCAAAGAG CTGACTTACC AGTCAGAGGA AGATAAGAAG 
AATCTGGCCA GGATGCAGGA TCTGATTGAC AAGCTACAGT TAAAAGTGAA GAGCTACAAG 
CACCAGGCAG AAGAAGCTGA AGCTCAAGCC AACCTGTACC TTTCGAAGTA CAGAAAACAG 
CAACATGATC TGGATGATGC TGAAGAACGG GCTGAAATAG CTGAATCTCA AGTTAATAAG 
CTGAGGAGCA AGTCAAGAGA TATTGGCATG AAAAAGGTTC ATGAAGAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MYH15 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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