SH3TC2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol SH3TC2

Entrez Gene ID 106891259

Full Name SH3 domain and tetratricopeptide repeats 2

Gene Type protein-coding

Organism Calidris pugnax(ruff)

mRNA	Protein	Name
XM_014947698.1	XP_014803184.1	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X3
XM_014947696.1	XP_014803182.1	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X2
XM_014947694.1	XP_014803180.1	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X1
XM_014947695.1	XP_014803181.1	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X2
XM_014947699.1	XP_014803185.1	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X4
XM_014947697.1	XP_014803183.1	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following SH3TC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SH3TC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa69212	XM_014947694.1 Latest version!	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa69213	XM_014947696.1 Latest version!	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa69213	XM_014947695.1 Latest version!	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa69213	XM_014947697.1 Latest version!	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa69214	XM_014947698.1 Latest version!	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa69215	XM_014947699.1 Latest version!	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SH3TC2 ( XM_014947694.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014803180.1 Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa69212
Clone ID Related Accession (Same CDS sequence)	XM_014947694.1
Accession Version	XM_014947694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCT GCTTGGGGAC CCCCTGGCAC CAGGACCTGA CCCTGGGGTG CACAGGCGAA 
GAGTGTGACT CCTGCTGCCA CCGCTCACCC GTGCTCCTGC CCCGCTGGGA CCTCCTGCAG 
CGCACCGCAG CCGAGCCCCG GGGAAAATGG TGGTGGGAGA AGACCATGGC ATCAGGTAAG 
GCTGTGGTAT CAGAGCCAAG CCCCCCTGCT ACCGAGGCAG CAGAAGGCTC ACCGGACACC 
TCCTCGGCGC TGCTGGCTGT CGGGGAGGGC TTTCCACCAG AGATTTCGCT CTTCTTCTCC 
ATTGAGAGCC GCTCCTCACA GTGCCTCAAT CCCCAGCTCC AGGAAGCAGC CAGAAAGAAG 
CTGTGGGCCC TGGAGAGTAA TGACAGAGAC GTTTGTGCCC TGTTCAAGGA GCTGTCAGCC 
AGGCTGGTCT GTATGCAAGC ACAGGAGGAC AGGTTCCTCC TCACCTTCAA AACCCTGGAA 
GAAGTCTGGA AGTTTTCTAC CTATCTGACT TTAGGGTACG TGGGCAGCTG CCTGGAACAG 
CTTCTCTTTG ACCAGGAATA CTGGCTAAAC TGTGCTCTGA TGGAAGACAC AGAGGTCAGA 
GTTACTGTGG ATGAAGATCG CTTGGCCACC ATATATATGG GTCTGCTGCT CCAAGAAGGT 
AGCTTTTTTT CCAGAGCAGT GCCCGGTGTC TGGCAGCCAG AGCAGGAGGG AGAGGAAGGT 
CTGCAGCTCT GCAAGAACGA GCTGATCCAT GTGAAGAGCG TTGGAGAGGA GTCCAAGTGG 
GAAGGGATGT CCTTACTGAC AGGTCAACGA GGCCTTGTGC CTGTGACAGC TCTAGAGCCA 
ATACCTCATC CATTTTACCA GTGGTTCCTG AAGAATTATG CTGTGAGTTT TGGCATCTCC 
CAAGAGATCA GTGGGACAAC TTCTCAGCCA ATTGTCAAAG GCAGGTGCAC AGCCACAGAG 
GACCACAGAG GAGCAGCACC GGATGAACTG AGTTTCTCCA AAGGAGACAG CATAGAAGTC 
ATCGGCTTCC TCATTCCAGG ACTCCCATGG TTTGTGGGCA AATCCGTCAG CAGTGGGAGC 
ATTGGCTTTG TCCCAACCCG ATACATAAAT CCTGAGGCTT GTGAACCTCT GGGAAAAGGC 
TTGGTGTTTC TGAGTGAAGA AGAAAAATCC CCGCTCCTGC GAGTCCCCTG TAATGGTGGG 
GAACAGCACT TCACCACCCT CCTCGGTGAC CTGGCACACA CCGACATCAC CTCTGTGTAC 
CGCCTGGATG GTTTTGAACC TAAAGCCATG TTCACAAAAG TGCCATCAGA GGCTGTTCTC 
CATGGCTGTA AAGATATCCA GCTGCTCCAA TCTTGGGAGG AAATAAATGG CTTGGCTACA 
ACTAGCACCT CCGAGCTGTC TAGCCCAGGG AGTGAATCCT CCCCTGCTAC ATTGGAAGAT 
GTTCTCCTGG AGAAGATGGA TGATTTTGAT GATCCTAAGT TCTTTATTGA CCTGAACGCT 
GGACACATGG AAGATGCTGA TGTCTTTGAC CCCATATTGA CCTTCCTTAA CCAAGAGAGT 
TATATGCCCA GTTTCCAAAG CCTCTATGAT CTCAGTTTTT CCTTTCTCAA CTCCACTTTT 
TATGGTTTCT CTGATGAGGA TGAACTGGTC TTGTACCTTG AGACATCCAG GAACTGGGCC 
AAGAGGACTC ATTCCGTTTG GGCTCATGTC AGGCTCTGTT TCCTCTTGGG TAAGCTCTGC 
ATCAAAAAGG TCAAGTTCTC CCAGGCTCGG GTCTACTTTG AGGAAGCCAT GAGCATCCTG 
GATAGGGGTT TTGGGGATCT GCCCCTCTTG GCTGCATTGC ATGTGAACCT TGCCTCAATC 
TACTTGAAAC AAAATATGAA GCACAAGTTC TCCGCCTTGA TGGGGAAAAC GGTGGCCTTG 
CTTGTCTGCT TGCCTGGCCA CTCTTTCAGC TCTGAGAATG AGCTGGAAGT CATGATGTAT 
GTCCTGAAGG AAGCTATTGC TGTGGGTAAT GCTCCCTTGG AGGCACGGGT CTGCTTCCTT 
ATTGTCAAGC TCTTCCTACA ACTGGGAAAA AACGATGAAG TGCTGCCCTT TACCGAGCAT 
CTTCAATGCC TCACCAACAC TTTACTCAGA CCAGACACCA GTTCTGTGCC ACTGGATGTA 
ACCCCCATCT TGAGCTACCT GTACGACAAG AAGTACTTGC CAAATATCGC ACTGGCATCT 
GCCAGGTTGT TTGTTCCCAG TGGCCTCAAG GGAGCACTGA CACCCATTTG GAGAGCTGGC 
TTCATCCTCC AAAGTATTTC CAAACTCCTG GGAAGCCACC TGGAGAGGAG TAGCATCCCA 
GCGCTGGCTT GTTTCTACCT CAAGCAAGCC CTGCATTTCT CCTGCGAGAC CAAAGCAGTG 
CCCACCCAGA GGACACTGTG TGCCATCTTG TCCAGGATGT ACCTCCAGCA CGGTGTCTTG 
GATGGGGCGG TTTGTTACGC AGCCACAGCT GTAACCCTCA GCAGACGGAT GGGCGAGGAG 
GAGGCTTTCG AGTCTTCCCT CTCCTTGGGG TGGATGTATC TCCTGAACAA CCGGCCGGGC 
CCAGCTGCAG ACATCATGTG GCAGCTCTTG CGCTCTCTAC ACGAGACGGA CAGCGTGACT 
CAGGGTGGAG CAGTGCACAA TCTCCTGGCC ATCGCCCTCA AAGGAGAAGG ACAGGTGCAA 
AAGGCAGCAG AGAACTACCT TCGGGCCCTG CACAAAGCCC AGGAGACTGG GAACAAGAGG 
AACCAGGCTA TTGCCCTGGC TAACCTGGGG CAGCTGAGCT CCTCGCGCGG GGCGAGCCAG 
CTGTCTGAGC TCTACCTGCT CCAGTCGGCT CAGCTCTACG CTGAGCTCCA GGGCAGCGAA 
GACCTGGAGA TGGAGTTGGT GCGGGTGTTG CTGTGGCTGG CACAGGCCAT GGTGAACAGG 
CTGAAGGTGG AAGATGGCAA ACTCTGTTAT GAACTGGCGT TGGTTTTTGC CCTGAAGTGG 
CATAACGTAA GGAGTCAGCT TCACGTCACT GAGTCTCTCT GCCATTTCTA CAGCAAGGTG 
TCCCCCAATC TCCAGGCCTG CATTACCTAC CATGAGCACT GGGTATCATT GGCACAACAG 
CTCCAGGACA GAGAGATGGA AGGCAATGCC CGGCAGACCC TCAGCCAGCT CTACCAGGCT 
TTGGGCACAT CTGAGGCTTT GAGACAGTCC CTGGACTGCA CCAAACAGAG TCTAAGGATC 
TTCATTGACC TTGAGGAGCC TGTGAAAGCA GCAGAGGCCT GGCTGCAGGC AGGAAGGCTC 
TACTATCTTA TGCAGGAAGA TGAGCTGGTG GAAATGTATT TTCAGGCAGC CATTCAGACT 
GCTCTGAAAT GGGAGAACTT CTCCTTGGCC ATGGACCTTT ATGAAAAAGC AGGTGATACC 
TTTTTTAATG GCAACCGAAA CAGAGATCGA GCTGTGGAGT TTTATAGGGG CGGTGCTGTG 
CCCTTGGCCA GGAAACTGAA GTCCAGTAGG ACAGAGCTGC GGCTGTTCAA TAAGCTGGCA 
GAGCTGCAGA TCGGGCTGCA GGGCTACGAG AAGGCACTGG AGTTTGCCAC ACTGGCAGCC 
AGGCTAAGCA TCAGGGTCGG AGATCAATTG CAAGAGCTGG TTGCATTCCA CCGCCTGGCG 
ACAGTCTACT ATTTTCTGCA TATGTATGAG ATGGCAGAAG ACTGCTACCT GAAGACCCTT 
GCCTTGCGTC CCCCCTTGCT GCAGTGCTCT GGAGAGGCCC TGTACTACTG CAAGATGTAT 
TGCCACCTTG GCAACCTGAC CCTGCACAAA CTGAAGGATG AACAGGATGC AGCAACCTAC 
TTCCTCCTGG CCCTCGCCGC AGCAACCGAG CTGGGAGACC AGGAGCTGCA AGACTTCATT 
CACGCCAAGC TGGGTGACAT CCCCGGTGCC CCGTGGGGGC CCGAGGGCAC GCCGGGCTGT 
GCCACATACC GGCCCAGGTG GCTGAGTGAA GGTGGCCACG TTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014803180.1
CDS	20..4027
Translation	MGCCLGTPWHQDLTLGCTGEECDSCCHRSPVLLPRWDLLQRTAAEPRGKWWWEKTMASGKAVVSEPSPPATEAAEGSPDTSSALLAVGEGFPPEISLFFSIESRSSQCLNPQLQEAARKKLWALESNDRDVCALFKELSARLVCMQAQEDRFLLTFKTLEEVWKFSTYLTLGYVGSCLEQLLFDQEYWLNCALMEDTEVRVTVDEDRLATIYMGLLLQEGSFFSRAVPGVWQPEQEGEEGLQLCKNELIHVKSVGEESKWEGMSLLTGQRGLVPVTALEPIPHPFYQWFLKNYAVSFGISQEISGTTSQPIVKGRCTATEDHRGAAPDELSFSKGDSIEVIGFLIPGLPWFVGKSVSSGSIGFVPTRYINPEACEPLGKGLVFLSEEEKSPLLRVPCNGGEQHFTTLLGDLAHTDITSVYRLDGFEPKAMFTKVPSEAVLHGCKDIQLLQSWEEINGLATTSTSELSSPGSESSPATLEDVLLEKMDDFDDPKFFIDLNAGHMEDADVFDPILTFLNQESYMPSFQSLYDLSFSFLNSTFYGFSDEDELVLYLETSRNWAKRTHSVWAHVRLCFLLGKLCIKKVKFSQARVYFEEAMSILDRGFGDLPLLAALHVNLASIYLKQNMKHKFSALMGKTVALLVCLPGHSFSSENELEVMMYVLKEAIAVGNAPLEARVCFLIVKLFLQLGKNDEVLPFTEHLQCLTNTLLRPDTSSVPLDVTPILSYLYDKKYLPNIALASARLFVPSGLKGALTPIWRAGFILQSISKLLGSHLERSSIPALACFYLKQALHFSCETKAVPTQRTLCAILSRMYLQHGVLDGAVCYAATAVTLSRRMGEEEAFESSLSLGWMYLLNNRPGPAADIMWQLLRSLHETDSVTQGGAVHNLLAIALKGEGQVQKAAENYLRALHKAQETGNKRNQAIALANLGQLSSSRGASQLSELYLLQSAQLYAELQGSEDLEMELVRVLLWLAQAMVNRLKVEDGKLCYELALVFALKWHNVRSQLHVTESLCHFYSKVSPNLQACITYHEHWVSLAQQLQDREMEGNARQTLSQLYQALGTSEALRQSLDCTKQSLRIFIDLEEPVKAAEAWLQAGRLYYLMQEDELVEMYFQAAIQTALKWENFSLAMDLYEKAGDTFFNGNRNRDRAVEFYRGGAVPLARKLKSSRTELRLFNKLAELQIGLQGYEKALEFATLAARLSIRVGDQLQELVAFHRLATVYYFLHMYEMAEDCYLKTLALRPPLLQCSGEALYYCKMYCHLGNLTLHKLKDEQDAATYFLLALAAATELGDQELQDFIHAKLGDIPGAPWGPEGTPGCATYRPRWLSEGGHVV

Target ORF information:

RefSeq Version	XM_014947694.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014947694.1

ORF Insert Sequence:

ATGGGCTGCT GCTTGGGGAC CCCCTGGCAC CAGGACCTGA CCCTGGGGTG CACAGGCGAA 
GAGTGTGACT CCTGCTGCCA CCGCTCACCC GTGCTCCTGC CCCGCTGGGA CCTCCTGCAG 
CGCACCGCAG CCGAGCCCCG GGGAAAATGG TGGTGGGAGA AGACCATGGC ATCAGGTAAG 
GCTGTGGTAT CAGAGCCAAG CCCCCCTGCT ACCGAGGCAG CAGAAGGCTC ACCGGACACC 
TCCTCGGCGC TGCTGGCTGT CGGGGAGGGC TTTCCACCAG AGATTTCGCT CTTCTTCTCC 
ATTGAGAGCC GCTCCTCACA GTGCCTCAAT CCCCAGCTCC AGGAAGCAGC CAGAAAGAAG 
CTGTGGGCCC TGGAGAGTAA TGACAGAGAC GTTTGTGCCC TGTTCAAGGA GCTGTCAGCC 
AGGCTGGTCT GTATGCAAGC ACAGGAGGAC AGGTTCCTCC TCACCTTCAA AACCCTGGAA 
GAAGTCTGGA AGTTTTCTAC CTATCTGACT TTAGGGTACG TGGGCAGCTG CCTGGAACAG 
CTTCTCTTTG ACCAGGAATA CTGGCTAAAC TGTGCTCTGA TGGAAGACAC AGAGGTCAGA 
GTTACTGTGG ATGAAGATCG CTTGGCCACC ATATATATGG GTCTGCTGCT CCAAGAAGGT 
AGCTTTTTTT CCAGAGCAGT GCCCGGTGTC TGGCAGCCAG AGCAGGAGGG AGAGGAAGGT 
CTGCAGCTCT GCAAGAACGA GCTGATCCAT GTGAAGAGCG TTGGAGAGGA GTCCAAGTGG 
GAAGGGATGT CCTTACTGAC AGGTCAACGA GGCCTTGTGC CTGTGACAGC TCTAGAGCCA 
ATACCTCATC CATTTTACCA GTGGTTCCTG AAGAATTATG CTGTGAGTTT TGGCATCTCC 
CAAGAGATCA GTGGGACAAC TTCTCAGCCA ATTGTCAAAG GCAGGTGCAC AGCCACAGAG 
GACCACAGAG GAGCAGCACC GGATGAACTG AGTTTCTCCA AAGGAGACAG CATAGAAGTC 
ATCGGCTTCC TCATTCCAGG ACTCCCATGG TTTGTGGGCA AATCCGTCAG CAGTGGGAGC 
ATTGGCTTTG TCCCAACCCG ATACATAAAT CCTGAGGCTT GTGAACCTCT GGGAAAAGGC 
TTGGTGTTTC TGAGTGAAGA AGAAAAATCC CCGCTCCTGC GAGTCCCCTG TAATGGTGGG 
GAACAGCACT TCACCACCCT CCTCGGTGAC CTGGCACACA CCGACATCAC CTCTGTGTAC 
CGCCTGGATG GTTTTGAACC TAAAGCCATG TTCACAAAAG TGCCATCAGA GGCTGTTCTC 
CATGGCTGTA AAGATATCCA GCTGCTCCAA TCTTGGGAGG AAATAAATGG CTTGGCTACA 
ACTAGCACCT CCGAGCTGTC TAGCCCAGGG AGTGAATCCT CCCCTGCTAC ATTGGAAGAT 
GTTCTCCTGG AGAAGATGGA TGATTTTGAT GATCCTAAGT TCTTTATTGA CCTGAACGCT 
GGACACATGG AAGATGCTGA TGTCTTTGAC CCCATATTGA CCTTCCTTAA CCAAGAGAGT 
TATATGCCCA GTTTCCAAAG CCTCTATGAT CTCAGTTTTT CCTTTCTCAA CTCCACTTTT 
TATGGTTTCT CTGATGAGGA TGAACTGGTC TTGTACCTTG AGACATCCAG GAACTGGGCC 
AAGAGGACTC ATTCCGTTTG GGCTCATGTC AGGCTCTGTT TCCTCTTGGG TAAGCTCTGC 
ATCAAAAAGG TCAAGTTCTC CCAGGCTCGG GTCTACTTTG AGGAAGCCAT GAGCATCCTG 
GATAGGGGTT TTGGGGATCT GCCCCTCTTG GCTGCATTGC ATGTGAACCT TGCCTCAATC 
TACTTGAAAC AAAATATGAA GCACAAGTTC TCCGCCTTGA TGGGGAAAAC GGTGGCCTTG 
CTTGTCTGCT TGCCTGGCCA CTCTTTCAGC TCTGAGAATG AGCTGGAAGT CATGATGTAT 
GTCCTGAAGG AAGCTATTGC TGTGGGTAAT GCTCCCTTGG AGGCACGGGT CTGCTTCCTT 
ATTGTCAAGC TCTTCCTACA ACTGGGAAAA AACGATGAAG TGCTGCCCTT TACCGAGCAT 
CTTCAATGCC TCACCAACAC TTTACTCAGA CCAGACACCA GTTCTGTGCC ACTGGATGTA 
ACCCCCATCT TGAGCTACCT GTACGACAAG AAGTACTTGC CAAATATCGC ACTGGCATCT 
GCCAGGTTGT TTGTTCCCAG TGGCCTCAAG GGAGCACTGA CACCCATTTG GAGAGCTGGC 
TTCATCCTCC AAAGTATTTC CAAACTCCTG GGAAGCCACC TGGAGAGGAG TAGCATCCCA 
GCGCTGGCTT GTTTCTACCT CAAGCAAGCC CTGCATTTCT CCTGCGAGAC CAAAGCAGTG 
CCCACCCAGA GGACACTGTG TGCCATCTTG TCCAGGATGT ACCTCCAGCA CGGTGTCTTG 
GATGGGGCGG TTTGTTACGC AGCCACAGCT GTAACCCTCA GCAGACGGAT GGGCGAGGAG 
GAGGCTTTCG AGTCTTCCCT CTCCTTGGGG TGGATGTATC TCCTGAACAA CCGGCCGGGC 
CCAGCTGCAG ACATCATGTG GCAGCTCTTG CGCTCTCTAC ACGAGACGGA CAGCGTGACT 
CAGGGTGGAG CAGTGCACAA TCTCCTGGCC ATCGCCCTCA AAGGAGAAGG ACAGGTGCAA 
AAGGCAGCAG AGAACTACCT TCGGGCCCTG CACAAAGCCC AGGAGACTGG GAACAAGAGG 
AACCAGGCTA TTGCCCTGGC TAACCTGGGG CAGCTGAGCT CCTCGCGCGG GGCGAGCCAG 
CTGTCTGAGC TCTACCTGCT CCAGTCGGCT CAGCTCTACG CTGAGCTCCA GGGCAGCGAA 
GACCTGGAGA TGGAGTTGGT GCGGGTGTTG CTGTGGCTGG CACAGGCCAT GGTGAACAGG 
CTGAAGGTGG AAGATGGCAA ACTCTGTTAT GAACTGGCGT TGGTTTTTGC CCTGAAGTGG 
CATAACGTAA GGAGTCAGCT TCACGTCACT GAGTCTCTCT GCCATTTCTA CAGCAAGGTG 
TCCCCCAATC TCCAGGCCTG CATTACCTAC CATGAGCACT GGGTATCATT GGCACAACAG 
CTCCAGGACA GAGAGATGGA AGGCAATGCC CGGCAGACCC TCAGCCAGCT CTACCAGGCT 
TTGGGCACAT CTGAGGCTTT GAGACAGTCC CTGGACTGCA CCAAACAGAG TCTAAGGATC 
TTCATTGACC TTGAGGAGCC TGTGAAAGCA GCAGAGGCCT GGCTGCAGGC AGGAAGGCTC 
TACTATCTTA TGCAGGAAGA TGAGCTGGTG GAAATGTATT TTCAGGCAGC CATTCAGACT 
GCTCTGAAAT GGGAGAACTT CTCCTTGGCC ATGGACCTTT ATGAAAAAGC AGGTGATACC 
TTTTTTAATG GCAACCGAAA CAGAGATCGA GCTGTGGAGT TTTATAGGGG CGGTGCTGTG 
CCCTTGGCCA GGAAACTGAA GTCCAGTAGG ACAGAGCTGC GGCTGTTCAA TAAGCTGGCA 
GAGCTGCAGA TCGGGCTGCA GGGCTACGAG AAGGCACTGG AGTTTGCCAC ACTGGCAGCC 
AGGCTAAGCA TCAGGGTCGG AGATCAATTG CAAGAGCTGG TTGCATTCCA CCGCCTGGCG 
ACAGTCTACT ATTTTCTGCA TATGTATGAG ATGGCAGAAG ACTGCTACCT GAAGACCCTT 
GCCTTGCGTC CCCCCTTGCT GCAGTGCTCT GGAGAGGCCC TGTACTACTG CAAGATGTAT 
TGCCACCTTG GCAACCTGAC CCTGCACAAA CTGAAGGATG AACAGGATGC AGCAACCTAC 
TTCCTCCTGG CCCTCGCCGC AGCAACCGAG CTGGGAGACC AGGAGCTGCA AGACTTCATT 
CACGCCAAGC TGGGTGACAT CCCCGGTGCC CCGTGGGGGC CCGAGGGCAC GCCGGGCTGT 
GCCACATACC GGCCCAGGTG GCTGAGTGAA GGTGGCCACG TTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3TC2 ( XM_014947696.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014803182.1 Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCa69213
Clone ID Related Accession (Same CDS sequence)	XM_014947696.1 , XM_014947695.1 , XM_014947697.1
Accession Version	XM_014947696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGACA ATCCCCAGCT GGGCATCCCT GCAGGCGAAG AGTGTGACTC CTGCTGCCAC 
CGCTCACCCG TGCTCCTGCC CCGCTGGGAC CTCCTGCAGC GCACCGCAGC CGAGCCCCGG 
GGAAAATGGT GGTGGGAGAA GACCATGGCA TCAGGTAAGG CTGTGGTATC AGAGCCAAGC 
CCCCCTGCTA CCGAGGCAGC AGAAGGCTCA CCGGACACCT CCTCGGCGCT GCTGGCTGTC 
GGGGAGGGCT TTCCACCAGA GATTTCGCTC TTCTTCTCCA TTGAGAGCCG CTCCTCACAG 
TGCCTCAATC CCCAGCTCCA GGAAGCAGCC AGAAAGAAGC TGTGGGCCCT GGAGAGTAAT 
GACAGAGACG TTTGTGCCCT GTTCAAGGAG CTGTCAGCCA GGCTGGTCTG TATGCAAGCA 
CAGGAGGACA GGTTCCTCCT CACCTTCAAA ACCCTGGAAG AAGTCTGGAA GTTTTCTACC 
TATCTGACTT TAGGGTACGT GGGCAGCTGC CTGGAACAGC TTCTCTTTGA CCAGGAATAC 
TGGCTAAACT GTGCTCTGAT GGAAGACACA GAGGTCAGAG TTACTGTGGA TGAAGATCGC 
TTGGCCACCA TATATATGGG TCTGCTGCTC CAAGAAGGTA GCTTTTTTTC CAGAGCAGTG 
CCCGGTGTCT GGCAGCCAGA GCAGGAGGGA GAGGAAGGTC TGCAGCTCTG CAAGAACGAG 
CTGATCCATG TGAAGAGCGT TGGAGAGGAG TCCAAGTGGG AAGGGATGTC CTTACTGACA 
GGTCAACGAG GCCTTGTGCC TGTGACAGCT CTAGAGCCAA TACCTCATCC ATTTTACCAG 
TGGTTCCTGA AGAATTATGC TGTGAGTTTT GGCATCTCCC AAGAGATCAG TGGGACAACT 
TCTCAGCCAA TTGTCAAAGG CAGGTGCACA GCCACAGAGG ACCACAGAGG AGCAGCACCG 
GATGAACTGA GTTTCTCCAA AGGAGACAGC ATAGAAGTCA TCGGCTTCCT CATTCCAGGA 
CTCCCATGGT TTGTGGGCAA ATCCGTCAGC AGTGGGAGCA TTGGCTTTGT CCCAACCCGA 
TACATAAATC CTGAGGCTTG TGAACCTCTG GGAAAAGGCT TGGTGTTTCT GAGTGAAGAA 
GAAAAATCCC CGCTCCTGCG AGTCCCCTGT AATGGTGGGG AACAGCACTT CACCACCCTC 
CTCGGTGACC TGGCACACAC CGACATCACC TCTGTGTACC GCCTGGATGG TTTTGAACCT 
AAAGCCATGT TCACAAAAGT GCCATCAGAG GCTGTTCTCC ATGGCTGTAA AGATATCCAG 
CTGCTCCAAT CTTGGGAGGA AATAAATGGC TTGGCTACAA CTAGCACCTC CGAGCTGTCT 
AGCCCAGGGA GTGAATCCTC CCCTGCTACA TTGGAAGATG TTCTCCTGGA GAAGATGGAT 
GATTTTGATG ATCCTAAGTT CTTTATTGAC CTGAACGCTG GACACATGGA AGATGCTGAT 
GTCTTTGACC CCATATTGAC CTTCCTTAAC CAAGAGAGTT ATATGCCCAG TTTCCAAAGC 
CTCTATGATC TCAGTTTTTC CTTTCTCAAC TCCACTTTTT ATGGTTTCTC TGATGAGGAT 
GAACTGGTCT TGTACCTTGA GACATCCAGG AACTGGGCCA AGAGGACTCA TTCCGTTTGG 
GCTCATGTCA GGCTCTGTTT CCTCTTGGGT AAGCTCTGCA TCAAAAAGGT CAAGTTCTCC 
CAGGCTCGGG TCTACTTTGA GGAAGCCATG AGCATCCTGG ATAGGGGTTT TGGGGATCTG 
CCCCTCTTGG CTGCATTGCA TGTGAACCTT GCCTCAATCT ACTTGAAACA AAATATGAAG 
CACAAGTTCT CCGCCTTGAT GGGGAAAACG GTGGCCTTGC TTGTCTGCTT GCCTGGCCAC 
TCTTTCAGCT CTGAGAATGA GCTGGAAGTC ATGATGTATG TCCTGAAGGA AGCTATTGCT 
GTGGGTAATG CTCCCTTGGA GGCACGGGTC TGCTTCCTTA TTGTCAAGCT CTTCCTACAA 
CTGGGAAAAA ACGATGAAGT GCTGCCCTTT ACCGAGCATC TTCAATGCCT CACCAACACT 
TTACTCAGAC CAGACACCAG TTCTGTGCCA CTGGATGTAA CCCCCATCTT GAGCTACCTG 
TACGACAAGA AGTACTTGCC AAATATCGCA CTGGCATCTG CCAGGTTGTT TGTTCCCAGT 
GGCCTCAAGG GAGCACTGAC ACCCATTTGG AGAGCTGGCT TCATCCTCCA AAGTATTTCC 
AAACTCCTGG GAAGCCACCT GGAGAGGAGT AGCATCCCAG CGCTGGCTTG TTTCTACCTC 
AAGCAAGCCC TGCATTTCTC CTGCGAGACC AAAGCAGTGC CCACCCAGAG GACACTGTGT 
GCCATCTTGT CCAGGATGTA CCTCCAGCAC GGTGTCTTGG ATGGGGCGGT TTGTTACGCA 
GCCACAGCTG TAACCCTCAG CAGACGGATG GGCGAGGAGG AGGCTTTCGA GTCTTCCCTC 
TCCTTGGGGT GGATGTATCT CCTGAACAAC CGGCCGGGCC CAGCTGCAGA CATCATGTGG 
CAGCTCTTGC GCTCTCTACA CGAGACGGAC AGCGTGACTC AGGGTGGAGC AGTGCACAAT 
CTCCTGGCCA TCGCCCTCAA AGGAGAAGGA CAGGTGCAAA AGGCAGCAGA GAACTACCTT 
CGGGCCCTGC ACAAAGCCCA GGAGACTGGG AACAAGAGGA ACCAGGCTAT TGCCCTGGCT 
AACCTGGGGC AGCTGAGCTC CTCGCGCGGG GCGAGCCAGC TGTCTGAGCT CTACCTGCTC 
CAGTCGGCTC AGCTCTACGC TGAGCTCCAG GGCAGCGAAG ACCTGGAGAT GGAGTTGGTG 
CGGGTGTTGC TGTGGCTGGC ACAGGCCATG GTGAACAGGC TGAAGGTGGA AGATGGCAAA 
CTCTGTTATG AACTGGCGTT GGTTTTTGCC CTGAAGTGGC ATAACGTAAG GAGTCAGCTT 
CACGTCACTG AGTCTCTCTG CCATTTCTAC AGCAAGGTGT CCCCCAATCT CCAGGCCTGC 
ATTACCTACC ATGAGCACTG GGTATCATTG GCACAACAGC TCCAGGACAG AGAGATGGAA 
GGCAATGCCC GGCAGACCCT CAGCCAGCTC TACCAGGCTT TGGGCACATC TGAGGCTTTG 
AGACAGTCCC TGGACTGCAC CAAACAGAGT CTAAGGATCT TCATTGACCT TGAGGAGCCT 
GTGAAAGCAG CAGAGGCCTG GCTGCAGGCA GGAAGGCTCT ACTATCTTAT GCAGGAAGAT 
GAGCTGGTGG AAATGTATTT TCAGGCAGCC ATTCAGACTG CTCTGAAATG GGAGAACTTC 
TCCTTGGCCA TGGACCTTTA TGAAAAAGCA GGTGATACCT TTTTTAATGG CAACCGAAAC 
AGAGATCGAG CTGTGGAGTT TTATAGGGGC GGTGCTGTGC CCTTGGCCAG GAAACTGAAG 
TCCAGTAGGA CAGAGCTGCG GCTGTTCAAT AAGCTGGCAG AGCTGCAGAT CGGGCTGCAG 
GGCTACGAGA AGGCACTGGA GTTTGCCACA CTGGCAGCCA GGCTAAGCAT CAGGGTCGGA 
GATCAATTGC AAGAGCTGGT TGCATTCCAC CGCCTGGCGA CAGTCTACTA TTTTCTGCAT 
ATGTATGAGA TGGCAGAAGA CTGCTACCTG AAGACCCTTG CCTTGCGTCC CCCCTTGCTG 
CAGTGCTCTG GAGAGGCCCT GTACTACTGC AAGATGTATT GCCACCTTGG CAACCTGACC 
CTGCACAAAC TGAAGGATGA ACAGGATGCA GCAACCTACT TCCTCCTGGC CCTCGCCGCA 
GCAACCGAGC TGGGAGACCA GGAGCTGCAA GACTTCATTC ACGCCAAGCT GGGTGACATC 
CCCGGTGCCC CGTGGGGGCC CGAGGGCACG CCGGGCTGTG CCACATACCG GCCCAGGTGG 
CTGAGTGAAG GTGGCCACGT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014803182.1
CDS	462..4448
Translation	MLDNPQLGIPAGEECDSCCHRSPVLLPRWDLLQRTAAEPRGKWWWEKTMASGKAVVSEPSPPATEAAEGSPDTSSALLAVGEGFPPEISLFFSIESRSSQCLNPQLQEAARKKLWALESNDRDVCALFKELSARLVCMQAQEDRFLLTFKTLEEVWKFSTYLTLGYVGSCLEQLLFDQEYWLNCALMEDTEVRVTVDEDRLATIYMGLLLQEGSFFSRAVPGVWQPEQEGEEGLQLCKNELIHVKSVGEESKWEGMSLLTGQRGLVPVTALEPIPHPFYQWFLKNYAVSFGISQEISGTTSQPIVKGRCTATEDHRGAAPDELSFSKGDSIEVIGFLIPGLPWFVGKSVSSGSIGFVPTRYINPEACEPLGKGLVFLSEEEKSPLLRVPCNGGEQHFTTLLGDLAHTDITSVYRLDGFEPKAMFTKVPSEAVLHGCKDIQLLQSWEEINGLATTSTSELSSPGSESSPATLEDVLLEKMDDFDDPKFFIDLNAGHMEDADVFDPILTFLNQESYMPSFQSLYDLSFSFLNSTFYGFSDEDELVLYLETSRNWAKRTHSVWAHVRLCFLLGKLCIKKVKFSQARVYFEEAMSILDRGFGDLPLLAALHVNLASIYLKQNMKHKFSALMGKTVALLVCLPGHSFSSENELEVMMYVLKEAIAVGNAPLEARVCFLIVKLFLQLGKNDEVLPFTEHLQCLTNTLLRPDTSSVPLDVTPILSYLYDKKYLPNIALASARLFVPSGLKGALTPIWRAGFILQSISKLLGSHLERSSIPALACFYLKQALHFSCETKAVPTQRTLCAILSRMYLQHGVLDGAVCYAATAVTLSRRMGEEEAFESSLSLGWMYLLNNRPGPAADIMWQLLRSLHETDSVTQGGAVHNLLAIALKGEGQVQKAAENYLRALHKAQETGNKRNQAIALANLGQLSSSRGASQLSELYLLQSAQLYAELQGSEDLEMELVRVLLWLAQAMVNRLKVEDGKLCYELALVFALKWHNVRSQLHVTESLCHFYSKVSPNLQACITYHEHWVSLAQQLQDREMEGNARQTLSQLYQALGTSEALRQSLDCTKQSLRIFIDLEEPVKAAEAWLQAGRLYYLMQEDELVEMYFQAAIQTALKWENFSLAMDLYEKAGDTFFNGNRNRDRAVEFYRGGAVPLARKLKSSRTELRLFNKLAELQIGLQGYEKALEFATLAARLSIRVGDQLQELVAFHRLATVYYFLHMYEMAEDCYLKTLALRPPLLQCSGEALYYCKMYCHLGNLTLHKLKDEQDAATYFLLALAAATELGDQELQDFIHAKLGDIPGAPWGPEGTPGCATYRPRWLSEGGHVV

Target ORF information:

RefSeq Version	XM_014947696.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014947696.1

ORF Insert Sequence:

ATGCTGGACA ATCCCCAGCT GGGCATCCCT GCAGGCGAAG AGTGTGACTC CTGCTGCCAC 
CGCTCACCCG TGCTCCTGCC CCGCTGGGAC CTCCTGCAGC GCACCGCAGC CGAGCCCCGG 
GGAAAATGGT GGTGGGAGAA GACCATGGCA TCAGGTAAGG CTGTGGTATC AGAGCCAAGC 
CCCCCTGCTA CCGAGGCAGC AGAAGGCTCA CCGGACACCT CCTCGGCGCT GCTGGCTGTC 
GGGGAGGGCT TTCCACCAGA GATTTCGCTC TTCTTCTCCA TTGAGAGCCG CTCCTCACAG 
TGCCTCAATC CCCAGCTCCA GGAAGCAGCC AGAAAGAAGC TGTGGGCCCT GGAGAGTAAT 
GACAGAGACG TTTGTGCCCT GTTCAAGGAG CTGTCAGCCA GGCTGGTCTG TATGCAAGCA 
CAGGAGGACA GGTTCCTCCT CACCTTCAAA ACCCTGGAAG AAGTCTGGAA GTTTTCTACC 
TATCTGACTT TAGGGTACGT GGGCAGCTGC CTGGAACAGC TTCTCTTTGA CCAGGAATAC 
TGGCTAAACT GTGCTCTGAT GGAAGACACA GAGGTCAGAG TTACTGTGGA TGAAGATCGC 
TTGGCCACCA TATATATGGG TCTGCTGCTC CAAGAAGGTA GCTTTTTTTC CAGAGCAGTG 
CCCGGTGTCT GGCAGCCAGA GCAGGAGGGA GAGGAAGGTC TGCAGCTCTG CAAGAACGAG 
CTGATCCATG TGAAGAGCGT TGGAGAGGAG TCCAAGTGGG AAGGGATGTC CTTACTGACA 
GGTCAACGAG GCCTTGTGCC TGTGACAGCT CTAGAGCCAA TACCTCATCC ATTTTACCAG 
TGGTTCCTGA AGAATTATGC TGTGAGTTTT GGCATCTCCC AAGAGATCAG TGGGACAACT 
TCTCAGCCAA TTGTCAAAGG CAGGTGCACA GCCACAGAGG ACCACAGAGG AGCAGCACCG 
GATGAACTGA GTTTCTCCAA AGGAGACAGC ATAGAAGTCA TCGGCTTCCT CATTCCAGGA 
CTCCCATGGT TTGTGGGCAA ATCCGTCAGC AGTGGGAGCA TTGGCTTTGT CCCAACCCGA 
TACATAAATC CTGAGGCTTG TGAACCTCTG GGAAAAGGCT TGGTGTTTCT GAGTGAAGAA 
GAAAAATCCC CGCTCCTGCG AGTCCCCTGT AATGGTGGGG AACAGCACTT CACCACCCTC 
CTCGGTGACC TGGCACACAC CGACATCACC TCTGTGTACC GCCTGGATGG TTTTGAACCT 
AAAGCCATGT TCACAAAAGT GCCATCAGAG GCTGTTCTCC ATGGCTGTAA AGATATCCAG 
CTGCTCCAAT CTTGGGAGGA AATAAATGGC TTGGCTACAA CTAGCACCTC CGAGCTGTCT 
AGCCCAGGGA GTGAATCCTC CCCTGCTACA TTGGAAGATG TTCTCCTGGA GAAGATGGAT 
GATTTTGATG ATCCTAAGTT CTTTATTGAC CTGAACGCTG GACACATGGA AGATGCTGAT 
GTCTTTGACC CCATATTGAC CTTCCTTAAC CAAGAGAGTT ATATGCCCAG TTTCCAAAGC 
CTCTATGATC TCAGTTTTTC CTTTCTCAAC TCCACTTTTT ATGGTTTCTC TGATGAGGAT 
GAACTGGTCT TGTACCTTGA GACATCCAGG AACTGGGCCA AGAGGACTCA TTCCGTTTGG 
GCTCATGTCA GGCTCTGTTT CCTCTTGGGT AAGCTCTGCA TCAAAAAGGT CAAGTTCTCC 
CAGGCTCGGG TCTACTTTGA GGAAGCCATG AGCATCCTGG ATAGGGGTTT TGGGGATCTG 
CCCCTCTTGG CTGCATTGCA TGTGAACCTT GCCTCAATCT ACTTGAAACA AAATATGAAG 
CACAAGTTCT CCGCCTTGAT GGGGAAAACG GTGGCCTTGC TTGTCTGCTT GCCTGGCCAC 
TCTTTCAGCT CTGAGAATGA GCTGGAAGTC ATGATGTATG TCCTGAAGGA AGCTATTGCT 
GTGGGTAATG CTCCCTTGGA GGCACGGGTC TGCTTCCTTA TTGTCAAGCT CTTCCTACAA 
CTGGGAAAAA ACGATGAAGT GCTGCCCTTT ACCGAGCATC TTCAATGCCT CACCAACACT 
TTACTCAGAC CAGACACCAG TTCTGTGCCA CTGGATGTAA CCCCCATCTT GAGCTACCTG 
TACGACAAGA AGTACTTGCC AAATATCGCA CTGGCATCTG CCAGGTTGTT TGTTCCCAGT 
GGCCTCAAGG GAGCACTGAC ACCCATTTGG AGAGCTGGCT TCATCCTCCA AAGTATTTCC 
AAACTCCTGG GAAGCCACCT GGAGAGGAGT AGCATCCCAG CGCTGGCTTG TTTCTACCTC 
AAGCAAGCCC TGCATTTCTC CTGCGAGACC AAAGCAGTGC CCACCCAGAG GACACTGTGT 
GCCATCTTGT CCAGGATGTA CCTCCAGCAC GGTGTCTTGG ATGGGGCGGT TTGTTACGCA 
GCCACAGCTG TAACCCTCAG CAGACGGATG GGCGAGGAGG AGGCTTTCGA GTCTTCCCTC 
TCCTTGGGGT GGATGTATCT CCTGAACAAC CGGCCGGGCC CAGCTGCAGA CATCATGTGG 
CAGCTCTTGC GCTCTCTACA CGAGACGGAC AGCGTGACTC AGGGTGGAGC AGTGCACAAT 
CTCCTGGCCA TCGCCCTCAA AGGAGAAGGA CAGGTGCAAA AGGCAGCAGA GAACTACCTT 
CGGGCCCTGC ACAAAGCCCA GGAGACTGGG AACAAGAGGA ACCAGGCTAT TGCCCTGGCT 
AACCTGGGGC AGCTGAGCTC CTCGCGCGGG GCGAGCCAGC TGTCTGAGCT CTACCTGCTC 
CAGTCGGCTC AGCTCTACGC TGAGCTCCAG GGCAGCGAAG ACCTGGAGAT GGAGTTGGTG 
CGGGTGTTGC TGTGGCTGGC ACAGGCCATG GTGAACAGGC TGAAGGTGGA AGATGGCAAA 
CTCTGTTATG AACTGGCGTT GGTTTTTGCC CTGAAGTGGC ATAACGTAAG GAGTCAGCTT 
CACGTCACTG AGTCTCTCTG CCATTTCTAC AGCAAGGTGT CCCCCAATCT CCAGGCCTGC 
ATTACCTACC ATGAGCACTG GGTATCATTG GCACAACAGC TCCAGGACAG AGAGATGGAA 
GGCAATGCCC GGCAGACCCT CAGCCAGCTC TACCAGGCTT TGGGCACATC TGAGGCTTTG 
AGACAGTCCC TGGACTGCAC CAAACAGAGT CTAAGGATCT TCATTGACCT TGAGGAGCCT 
GTGAAAGCAG CAGAGGCCTG GCTGCAGGCA GGAAGGCTCT ACTATCTTAT GCAGGAAGAT 
GAGCTGGTGG AAATGTATTT TCAGGCAGCC ATTCAGACTG CTCTGAAATG GGAGAACTTC 
TCCTTGGCCA TGGACCTTTA TGAAAAAGCA GGTGATACCT TTTTTAATGG CAACCGAAAC 
AGAGATCGAG CTGTGGAGTT TTATAGGGGC GGTGCTGTGC CCTTGGCCAG GAAACTGAAG 
TCCAGTAGGA CAGAGCTGCG GCTGTTCAAT AAGCTGGCAG AGCTGCAGAT CGGGCTGCAG 
GGCTACGAGA AGGCACTGGA GTTTGCCACA CTGGCAGCCA GGCTAAGCAT CAGGGTCGGA 
GATCAATTGC AAGAGCTGGT TGCATTCCAC CGCCTGGCGA CAGTCTACTA TTTTCTGCAT 
ATGTATGAGA TGGCAGAAGA CTGCTACCTG AAGACCCTTG CCTTGCGTCC CCCCTTGCTG 
CAGTGCTCTG GAGAGGCCCT GTACTACTGC AAGATGTATT GCCACCTTGG CAACCTGACC 
CTGCACAAAC TGAAGGATGA ACAGGATGCA GCAACCTACT TCCTCCTGGC CCTCGCCGCA 
GCAACCGAGC TGGGAGACCA GGAGCTGCAA GACTTCATTC ACGCCAAGCT GGGTGACATC 
CCCGGTGCCC CGTGGGGGCC CGAGGGCACG CCGGGCTGTG CCACATACCG GCCCAGGTGG 
CTGAGTGAAG GTGGCCACGT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3TC2 ( XM_014947695.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014803181.1 Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X2, mRNA.

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CloneID	OCa69213
Clone ID Related Accession (Same CDS sequence)	XM_014947696.1 , XM_014947695.1 , XM_014947697.1
Accession Version	XM_014947695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGACA ATCCCCAGCT GGGCATCCCT GCAGGCGAAG AGTGTGACTC CTGCTGCCAC 
CGCTCACCCG TGCTCCTGCC CCGCTGGGAC CTCCTGCAGC GCACCGCAGC CGAGCCCCGG 
GGAAAATGGT GGTGGGAGAA GACCATGGCA TCAGGTAAGG CTGTGGTATC AGAGCCAAGC 
CCCCCTGCTA CCGAGGCAGC AGAAGGCTCA CCGGACACCT CCTCGGCGCT GCTGGCTGTC 
GGGGAGGGCT TTCCACCAGA GATTTCGCTC TTCTTCTCCA TTGAGAGCCG CTCCTCACAG 
TGCCTCAATC CCCAGCTCCA GGAAGCAGCC AGAAAGAAGC TGTGGGCCCT GGAGAGTAAT 
GACAGAGACG TTTGTGCCCT GTTCAAGGAG CTGTCAGCCA GGCTGGTCTG TATGCAAGCA 
CAGGAGGACA GGTTCCTCCT CACCTTCAAA ACCCTGGAAG AAGTCTGGAA GTTTTCTACC 
TATCTGACTT TAGGGTACGT GGGCAGCTGC CTGGAACAGC TTCTCTTTGA CCAGGAATAC 
TGGCTAAACT GTGCTCTGAT GGAAGACACA GAGGTCAGAG TTACTGTGGA TGAAGATCGC 
TTGGCCACCA TATATATGGG TCTGCTGCTC CAAGAAGGTA GCTTTTTTTC CAGAGCAGTG 
CCCGGTGTCT GGCAGCCAGA GCAGGAGGGA GAGGAAGGTC TGCAGCTCTG CAAGAACGAG 
CTGATCCATG TGAAGAGCGT TGGAGAGGAG TCCAAGTGGG AAGGGATGTC CTTACTGACA 
GGTCAACGAG GCCTTGTGCC TGTGACAGCT CTAGAGCCAA TACCTCATCC ATTTTACCAG 
TGGTTCCTGA AGAATTATGC TGTGAGTTTT GGCATCTCCC AAGAGATCAG TGGGACAACT 
TCTCAGCCAA TTGTCAAAGG CAGGTGCACA GCCACAGAGG ACCACAGAGG AGCAGCACCG 
GATGAACTGA GTTTCTCCAA AGGAGACAGC ATAGAAGTCA TCGGCTTCCT CATTCCAGGA 
CTCCCATGGT TTGTGGGCAA ATCCGTCAGC AGTGGGAGCA TTGGCTTTGT CCCAACCCGA 
TACATAAATC CTGAGGCTTG TGAACCTCTG GGAAAAGGCT TGGTGTTTCT GAGTGAAGAA 
GAAAAATCCC CGCTCCTGCG AGTCCCCTGT AATGGTGGGG AACAGCACTT CACCACCCTC 
CTCGGTGACC TGGCACACAC CGACATCACC TCTGTGTACC GCCTGGATGG TTTTGAACCT 
AAAGCCATGT TCACAAAAGT GCCATCAGAG GCTGTTCTCC ATGGCTGTAA AGATATCCAG 
CTGCTCCAAT CTTGGGAGGA AATAAATGGC TTGGCTACAA CTAGCACCTC CGAGCTGTCT 
AGCCCAGGGA GTGAATCCTC CCCTGCTACA TTGGAAGATG TTCTCCTGGA GAAGATGGAT 
GATTTTGATG ATCCTAAGTT CTTTATTGAC CTGAACGCTG GACACATGGA AGATGCTGAT 
GTCTTTGACC CCATATTGAC CTTCCTTAAC CAAGAGAGTT ATATGCCCAG TTTCCAAAGC 
CTCTATGATC TCAGTTTTTC CTTTCTCAAC TCCACTTTTT ATGGTTTCTC TGATGAGGAT 
GAACTGGTCT TGTACCTTGA GACATCCAGG AACTGGGCCA AGAGGACTCA TTCCGTTTGG 
GCTCATGTCA GGCTCTGTTT CCTCTTGGGT AAGCTCTGCA TCAAAAAGGT CAAGTTCTCC 
CAGGCTCGGG TCTACTTTGA GGAAGCCATG AGCATCCTGG ATAGGGGTTT TGGGGATCTG 
CCCCTCTTGG CTGCATTGCA TGTGAACCTT GCCTCAATCT ACTTGAAACA AAATATGAAG 
CACAAGTTCT CCGCCTTGAT GGGGAAAACG GTGGCCTTGC TTGTCTGCTT GCCTGGCCAC 
TCTTTCAGCT CTGAGAATGA GCTGGAAGTC ATGATGTATG TCCTGAAGGA AGCTATTGCT 
GTGGGTAATG CTCCCTTGGA GGCACGGGTC TGCTTCCTTA TTGTCAAGCT CTTCCTACAA 
CTGGGAAAAA ACGATGAAGT GCTGCCCTTT ACCGAGCATC TTCAATGCCT CACCAACACT 
TTACTCAGAC CAGACACCAG TTCTGTGCCA CTGGATGTAA CCCCCATCTT GAGCTACCTG 
TACGACAAGA AGTACTTGCC AAATATCGCA CTGGCATCTG CCAGGTTGTT TGTTCCCAGT 
GGCCTCAAGG GAGCACTGAC ACCCATTTGG AGAGCTGGCT TCATCCTCCA AAGTATTTCC 
AAACTCCTGG GAAGCCACCT GGAGAGGAGT AGCATCCCAG CGCTGGCTTG TTTCTACCTC 
AAGCAAGCCC TGCATTTCTC CTGCGAGACC AAAGCAGTGC CCACCCAGAG GACACTGTGT 
GCCATCTTGT CCAGGATGTA CCTCCAGCAC GGTGTCTTGG ATGGGGCGGT TTGTTACGCA 
GCCACAGCTG TAACCCTCAG CAGACGGATG GGCGAGGAGG AGGCTTTCGA GTCTTCCCTC 
TCCTTGGGGT GGATGTATCT CCTGAACAAC CGGCCGGGCC CAGCTGCAGA CATCATGTGG 
CAGCTCTTGC GCTCTCTACA CGAGACGGAC AGCGTGACTC AGGGTGGAGC AGTGCACAAT 
CTCCTGGCCA TCGCCCTCAA AGGAGAAGGA CAGGTGCAAA AGGCAGCAGA GAACTACCTT 
CGGGCCCTGC ACAAAGCCCA GGAGACTGGG AACAAGAGGA ACCAGGCTAT TGCCCTGGCT 
AACCTGGGGC AGCTGAGCTC CTCGCGCGGG GCGAGCCAGC TGTCTGAGCT CTACCTGCTC 
CAGTCGGCTC AGCTCTACGC TGAGCTCCAG GGCAGCGAAG ACCTGGAGAT GGAGTTGGTG 
CGGGTGTTGC TGTGGCTGGC ACAGGCCATG GTGAACAGGC TGAAGGTGGA AGATGGCAAA 
CTCTGTTATG AACTGGCGTT GGTTTTTGCC CTGAAGTGGC ATAACGTAAG GAGTCAGCTT 
CACGTCACTG AGTCTCTCTG CCATTTCTAC AGCAAGGTGT CCCCCAATCT CCAGGCCTGC 
ATTACCTACC ATGAGCACTG GGTATCATTG GCACAACAGC TCCAGGACAG AGAGATGGAA 
GGCAATGCCC GGCAGACCCT CAGCCAGCTC TACCAGGCTT TGGGCACATC TGAGGCTTTG 
AGACAGTCCC TGGACTGCAC CAAACAGAGT CTAAGGATCT TCATTGACCT TGAGGAGCCT 
GTGAAAGCAG CAGAGGCCTG GCTGCAGGCA GGAAGGCTCT ACTATCTTAT GCAGGAAGAT 
GAGCTGGTGG AAATGTATTT TCAGGCAGCC ATTCAGACTG CTCTGAAATG GGAGAACTTC 
TCCTTGGCCA TGGACCTTTA TGAAAAAGCA GGTGATACCT TTTTTAATGG CAACCGAAAC 
AGAGATCGAG CTGTGGAGTT TTATAGGGGC GGTGCTGTGC CCTTGGCCAG GAAACTGAAG 
TCCAGTAGGA CAGAGCTGCG GCTGTTCAAT AAGCTGGCAG AGCTGCAGAT CGGGCTGCAG 
GGCTACGAGA AGGCACTGGA GTTTGCCACA CTGGCAGCCA GGCTAAGCAT CAGGGTCGGA 
GATCAATTGC AAGAGCTGGT TGCATTCCAC CGCCTGGCGA CAGTCTACTA TTTTCTGCAT 
ATGTATGAGA TGGCAGAAGA CTGCTACCTG AAGACCCTTG CCTTGCGTCC CCCCTTGCTG 
CAGTGCTCTG GAGAGGCCCT GTACTACTGC AAGATGTATT GCCACCTTGG CAACCTGACC 
CTGCACAAAC TGAAGGATGA ACAGGATGCA GCAACCTACT TCCTCCTGGC CCTCGCCGCA 
GCAACCGAGC TGGGAGACCA GGAGCTGCAA GACTTCATTC ACGCCAAGCT GGGTGACATC 
CCCGGTGCCC CGTGGGGGCC CGAGGGCACG CCGGGCTGTG CCACATACCG GCCCAGGTGG 
CTGAGTGAAG GTGGCCACGT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014803181.1
CDS	558..4544
Translation	MLDNPQLGIPAGEECDSCCHRSPVLLPRWDLLQRTAAEPRGKWWWEKTMASGKAVVSEPSPPATEAAEGSPDTSSALLAVGEGFPPEISLFFSIESRSSQCLNPQLQEAARKKLWALESNDRDVCALFKELSARLVCMQAQEDRFLLTFKTLEEVWKFSTYLTLGYVGSCLEQLLFDQEYWLNCALMEDTEVRVTVDEDRLATIYMGLLLQEGSFFSRAVPGVWQPEQEGEEGLQLCKNELIHVKSVGEESKWEGMSLLTGQRGLVPVTALEPIPHPFYQWFLKNYAVSFGISQEISGTTSQPIVKGRCTATEDHRGAAPDELSFSKGDSIEVIGFLIPGLPWFVGKSVSSGSIGFVPTRYINPEACEPLGKGLVFLSEEEKSPLLRVPCNGGEQHFTTLLGDLAHTDITSVYRLDGFEPKAMFTKVPSEAVLHGCKDIQLLQSWEEINGLATTSTSELSSPGSESSPATLEDVLLEKMDDFDDPKFFIDLNAGHMEDADVFDPILTFLNQESYMPSFQSLYDLSFSFLNSTFYGFSDEDELVLYLETSRNWAKRTHSVWAHVRLCFLLGKLCIKKVKFSQARVYFEEAMSILDRGFGDLPLLAALHVNLASIYLKQNMKHKFSALMGKTVALLVCLPGHSFSSENELEVMMYVLKEAIAVGNAPLEARVCFLIVKLFLQLGKNDEVLPFTEHLQCLTNTLLRPDTSSVPLDVTPILSYLYDKKYLPNIALASARLFVPSGLKGALTPIWRAGFILQSISKLLGSHLERSSIPALACFYLKQALHFSCETKAVPTQRTLCAILSRMYLQHGVLDGAVCYAATAVTLSRRMGEEEAFESSLSLGWMYLLNNRPGPAADIMWQLLRSLHETDSVTQGGAVHNLLAIALKGEGQVQKAAENYLRALHKAQETGNKRNQAIALANLGQLSSSRGASQLSELYLLQSAQLYAELQGSEDLEMELVRVLLWLAQAMVNRLKVEDGKLCYELALVFALKWHNVRSQLHVTESLCHFYSKVSPNLQACITYHEHWVSLAQQLQDREMEGNARQTLSQLYQALGTSEALRQSLDCTKQSLRIFIDLEEPVKAAEAWLQAGRLYYLMQEDELVEMYFQAAIQTALKWENFSLAMDLYEKAGDTFFNGNRNRDRAVEFYRGGAVPLARKLKSSRTELRLFNKLAELQIGLQGYEKALEFATLAARLSIRVGDQLQELVAFHRLATVYYFLHMYEMAEDCYLKTLALRPPLLQCSGEALYYCKMYCHLGNLTLHKLKDEQDAATYFLLALAAATELGDQELQDFIHAKLGDIPGAPWGPEGTPGCATYRPRWLSEGGHVV

Target ORF information:

RefSeq Version	XM_014947695.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014947695.1

ORF Insert Sequence:

ATGCTGGACA ATCCCCAGCT GGGCATCCCT GCAGGCGAAG AGTGTGACTC CTGCTGCCAC 
CGCTCACCCG TGCTCCTGCC CCGCTGGGAC CTCCTGCAGC GCACCGCAGC CGAGCCCCGG 
GGAAAATGGT GGTGGGAGAA GACCATGGCA TCAGGTAAGG CTGTGGTATC AGAGCCAAGC 
CCCCCTGCTA CCGAGGCAGC AGAAGGCTCA CCGGACACCT CCTCGGCGCT GCTGGCTGTC 
GGGGAGGGCT TTCCACCAGA GATTTCGCTC TTCTTCTCCA TTGAGAGCCG CTCCTCACAG 
TGCCTCAATC CCCAGCTCCA GGAAGCAGCC AGAAAGAAGC TGTGGGCCCT GGAGAGTAAT 
GACAGAGACG TTTGTGCCCT GTTCAAGGAG CTGTCAGCCA GGCTGGTCTG TATGCAAGCA 
CAGGAGGACA GGTTCCTCCT CACCTTCAAA ACCCTGGAAG AAGTCTGGAA GTTTTCTACC 
TATCTGACTT TAGGGTACGT GGGCAGCTGC CTGGAACAGC TTCTCTTTGA CCAGGAATAC 
TGGCTAAACT GTGCTCTGAT GGAAGACACA GAGGTCAGAG TTACTGTGGA TGAAGATCGC 
TTGGCCACCA TATATATGGG TCTGCTGCTC CAAGAAGGTA GCTTTTTTTC CAGAGCAGTG 
CCCGGTGTCT GGCAGCCAGA GCAGGAGGGA GAGGAAGGTC TGCAGCTCTG CAAGAACGAG 
CTGATCCATG TGAAGAGCGT TGGAGAGGAG TCCAAGTGGG AAGGGATGTC CTTACTGACA 
GGTCAACGAG GCCTTGTGCC TGTGACAGCT CTAGAGCCAA TACCTCATCC ATTTTACCAG 
TGGTTCCTGA AGAATTATGC TGTGAGTTTT GGCATCTCCC AAGAGATCAG TGGGACAACT 
TCTCAGCCAA TTGTCAAAGG CAGGTGCACA GCCACAGAGG ACCACAGAGG AGCAGCACCG 
GATGAACTGA GTTTCTCCAA AGGAGACAGC ATAGAAGTCA TCGGCTTCCT CATTCCAGGA 
CTCCCATGGT TTGTGGGCAA ATCCGTCAGC AGTGGGAGCA TTGGCTTTGT CCCAACCCGA 
TACATAAATC CTGAGGCTTG TGAACCTCTG GGAAAAGGCT TGGTGTTTCT GAGTGAAGAA 
GAAAAATCCC CGCTCCTGCG AGTCCCCTGT AATGGTGGGG AACAGCACTT CACCACCCTC 
CTCGGTGACC TGGCACACAC CGACATCACC TCTGTGTACC GCCTGGATGG TTTTGAACCT 
AAAGCCATGT TCACAAAAGT GCCATCAGAG GCTGTTCTCC ATGGCTGTAA AGATATCCAG 
CTGCTCCAAT CTTGGGAGGA AATAAATGGC TTGGCTACAA CTAGCACCTC CGAGCTGTCT 
AGCCCAGGGA GTGAATCCTC CCCTGCTACA TTGGAAGATG TTCTCCTGGA GAAGATGGAT 
GATTTTGATG ATCCTAAGTT CTTTATTGAC CTGAACGCTG GACACATGGA AGATGCTGAT 
GTCTTTGACC CCATATTGAC CTTCCTTAAC CAAGAGAGTT ATATGCCCAG TTTCCAAAGC 
CTCTATGATC TCAGTTTTTC CTTTCTCAAC TCCACTTTTT ATGGTTTCTC TGATGAGGAT 
GAACTGGTCT TGTACCTTGA GACATCCAGG AACTGGGCCA AGAGGACTCA TTCCGTTTGG 
GCTCATGTCA GGCTCTGTTT CCTCTTGGGT AAGCTCTGCA TCAAAAAGGT CAAGTTCTCC 
CAGGCTCGGG TCTACTTTGA GGAAGCCATG AGCATCCTGG ATAGGGGTTT TGGGGATCTG 
CCCCTCTTGG CTGCATTGCA TGTGAACCTT GCCTCAATCT ACTTGAAACA AAATATGAAG 
CACAAGTTCT CCGCCTTGAT GGGGAAAACG GTGGCCTTGC TTGTCTGCTT GCCTGGCCAC 
TCTTTCAGCT CTGAGAATGA GCTGGAAGTC ATGATGTATG TCCTGAAGGA AGCTATTGCT 
GTGGGTAATG CTCCCTTGGA GGCACGGGTC TGCTTCCTTA TTGTCAAGCT CTTCCTACAA 
CTGGGAAAAA ACGATGAAGT GCTGCCCTTT ACCGAGCATC TTCAATGCCT CACCAACACT 
TTACTCAGAC CAGACACCAG TTCTGTGCCA CTGGATGTAA CCCCCATCTT GAGCTACCTG 
TACGACAAGA AGTACTTGCC AAATATCGCA CTGGCATCTG CCAGGTTGTT TGTTCCCAGT 
GGCCTCAAGG GAGCACTGAC ACCCATTTGG AGAGCTGGCT TCATCCTCCA AAGTATTTCC 
AAACTCCTGG GAAGCCACCT GGAGAGGAGT AGCATCCCAG CGCTGGCTTG TTTCTACCTC 
AAGCAAGCCC TGCATTTCTC CTGCGAGACC AAAGCAGTGC CCACCCAGAG GACACTGTGT 
GCCATCTTGT CCAGGATGTA CCTCCAGCAC GGTGTCTTGG ATGGGGCGGT TTGTTACGCA 
GCCACAGCTG TAACCCTCAG CAGACGGATG GGCGAGGAGG AGGCTTTCGA GTCTTCCCTC 
TCCTTGGGGT GGATGTATCT CCTGAACAAC CGGCCGGGCC CAGCTGCAGA CATCATGTGG 
CAGCTCTTGC GCTCTCTACA CGAGACGGAC AGCGTGACTC AGGGTGGAGC AGTGCACAAT 
CTCCTGGCCA TCGCCCTCAA AGGAGAAGGA CAGGTGCAAA AGGCAGCAGA GAACTACCTT 
CGGGCCCTGC ACAAAGCCCA GGAGACTGGG AACAAGAGGA ACCAGGCTAT TGCCCTGGCT 
AACCTGGGGC AGCTGAGCTC CTCGCGCGGG GCGAGCCAGC TGTCTGAGCT CTACCTGCTC 
CAGTCGGCTC AGCTCTACGC TGAGCTCCAG GGCAGCGAAG ACCTGGAGAT GGAGTTGGTG 
CGGGTGTTGC TGTGGCTGGC ACAGGCCATG GTGAACAGGC TGAAGGTGGA AGATGGCAAA 
CTCTGTTATG AACTGGCGTT GGTTTTTGCC CTGAAGTGGC ATAACGTAAG GAGTCAGCTT 
CACGTCACTG AGTCTCTCTG CCATTTCTAC AGCAAGGTGT CCCCCAATCT CCAGGCCTGC 
ATTACCTACC ATGAGCACTG GGTATCATTG GCACAACAGC TCCAGGACAG AGAGATGGAA 
GGCAATGCCC GGCAGACCCT CAGCCAGCTC TACCAGGCTT TGGGCACATC TGAGGCTTTG 
AGACAGTCCC TGGACTGCAC CAAACAGAGT CTAAGGATCT TCATTGACCT TGAGGAGCCT 
GTGAAAGCAG CAGAGGCCTG GCTGCAGGCA GGAAGGCTCT ACTATCTTAT GCAGGAAGAT 
GAGCTGGTGG AAATGTATTT TCAGGCAGCC ATTCAGACTG CTCTGAAATG GGAGAACTTC 
TCCTTGGCCA TGGACCTTTA TGAAAAAGCA GGTGATACCT TTTTTAATGG CAACCGAAAC 
AGAGATCGAG CTGTGGAGTT TTATAGGGGC GGTGCTGTGC CCTTGGCCAG GAAACTGAAG 
TCCAGTAGGA CAGAGCTGCG GCTGTTCAAT AAGCTGGCAG AGCTGCAGAT CGGGCTGCAG 
GGCTACGAGA AGGCACTGGA GTTTGCCACA CTGGCAGCCA GGCTAAGCAT CAGGGTCGGA 
GATCAATTGC AAGAGCTGGT TGCATTCCAC CGCCTGGCGA CAGTCTACTA TTTTCTGCAT 
ATGTATGAGA TGGCAGAAGA CTGCTACCTG AAGACCCTTG CCTTGCGTCC CCCCTTGCTG 
CAGTGCTCTG GAGAGGCCCT GTACTACTGC AAGATGTATT GCCACCTTGG CAACCTGACC 
CTGCACAAAC TGAAGGATGA ACAGGATGCA GCAACCTACT TCCTCCTGGC CCTCGCCGCA 
GCAACCGAGC TGGGAGACCA GGAGCTGCAA GACTTCATTC ACGCCAAGCT GGGTGACATC 
CCCGGTGCCC CGTGGGGGCC CGAGGGCACG CCGGGCTGTG CCACATACCG GCCCAGGTGG 
CTGAGTGAAG GTGGCCACGT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3TC2 ( XM_014947697.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014803183.1 Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa69213
Clone ID Related Accession (Same CDS sequence)	XM_014947696.1 , XM_014947695.1 , XM_014947697.1
Accession Version	XM_014947697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGACA ATCCCCAGCT GGGCATCCCT GCAGGCGAAG AGTGTGACTC CTGCTGCCAC 
CGCTCACCCG TGCTCCTGCC CCGCTGGGAC CTCCTGCAGC GCACCGCAGC CGAGCCCCGG 
GGAAAATGGT GGTGGGAGAA GACCATGGCA TCAGGTAAGG CTGTGGTATC AGAGCCAAGC 
CCCCCTGCTA CCGAGGCAGC AGAAGGCTCA CCGGACACCT CCTCGGCGCT GCTGGCTGTC 
GGGGAGGGCT TTCCACCAGA GATTTCGCTC TTCTTCTCCA TTGAGAGCCG CTCCTCACAG 
TGCCTCAATC CCCAGCTCCA GGAAGCAGCC AGAAAGAAGC TGTGGGCCCT GGAGAGTAAT 
GACAGAGACG TTTGTGCCCT GTTCAAGGAG CTGTCAGCCA GGCTGGTCTG TATGCAAGCA 
CAGGAGGACA GGTTCCTCCT CACCTTCAAA ACCCTGGAAG AAGTCTGGAA GTTTTCTACC 
TATCTGACTT TAGGGTACGT GGGCAGCTGC CTGGAACAGC TTCTCTTTGA CCAGGAATAC 
TGGCTAAACT GTGCTCTGAT GGAAGACACA GAGGTCAGAG TTACTGTGGA TGAAGATCGC 
TTGGCCACCA TATATATGGG TCTGCTGCTC CAAGAAGGTA GCTTTTTTTC CAGAGCAGTG 
CCCGGTGTCT GGCAGCCAGA GCAGGAGGGA GAGGAAGGTC TGCAGCTCTG CAAGAACGAG 
CTGATCCATG TGAAGAGCGT TGGAGAGGAG TCCAAGTGGG AAGGGATGTC CTTACTGACA 
GGTCAACGAG GCCTTGTGCC TGTGACAGCT CTAGAGCCAA TACCTCATCC ATTTTACCAG 
TGGTTCCTGA AGAATTATGC TGTGAGTTTT GGCATCTCCC AAGAGATCAG TGGGACAACT 
TCTCAGCCAA TTGTCAAAGG CAGGTGCACA GCCACAGAGG ACCACAGAGG AGCAGCACCG 
GATGAACTGA GTTTCTCCAA AGGAGACAGC ATAGAAGTCA TCGGCTTCCT CATTCCAGGA 
CTCCCATGGT TTGTGGGCAA ATCCGTCAGC AGTGGGAGCA TTGGCTTTGT CCCAACCCGA 
TACATAAATC CTGAGGCTTG TGAACCTCTG GGAAAAGGCT TGGTGTTTCT GAGTGAAGAA 
GAAAAATCCC CGCTCCTGCG AGTCCCCTGT AATGGTGGGG AACAGCACTT CACCACCCTC 
CTCGGTGACC TGGCACACAC CGACATCACC TCTGTGTACC GCCTGGATGG TTTTGAACCT 
AAAGCCATGT TCACAAAAGT GCCATCAGAG GCTGTTCTCC ATGGCTGTAA AGATATCCAG 
CTGCTCCAAT CTTGGGAGGA AATAAATGGC TTGGCTACAA CTAGCACCTC CGAGCTGTCT 
AGCCCAGGGA GTGAATCCTC CCCTGCTACA TTGGAAGATG TTCTCCTGGA GAAGATGGAT 
GATTTTGATG ATCCTAAGTT CTTTATTGAC CTGAACGCTG GACACATGGA AGATGCTGAT 
GTCTTTGACC CCATATTGAC CTTCCTTAAC CAAGAGAGTT ATATGCCCAG TTTCCAAAGC 
CTCTATGATC TCAGTTTTTC CTTTCTCAAC TCCACTTTTT ATGGTTTCTC TGATGAGGAT 
GAACTGGTCT TGTACCTTGA GACATCCAGG AACTGGGCCA AGAGGACTCA TTCCGTTTGG 
GCTCATGTCA GGCTCTGTTT CCTCTTGGGT AAGCTCTGCA TCAAAAAGGT CAAGTTCTCC 
CAGGCTCGGG TCTACTTTGA GGAAGCCATG AGCATCCTGG ATAGGGGTTT TGGGGATCTG 
CCCCTCTTGG CTGCATTGCA TGTGAACCTT GCCTCAATCT ACTTGAAACA AAATATGAAG 
CACAAGTTCT CCGCCTTGAT GGGGAAAACG GTGGCCTTGC TTGTCTGCTT GCCTGGCCAC 
TCTTTCAGCT CTGAGAATGA GCTGGAAGTC ATGATGTATG TCCTGAAGGA AGCTATTGCT 
GTGGGTAATG CTCCCTTGGA GGCACGGGTC TGCTTCCTTA TTGTCAAGCT CTTCCTACAA 
CTGGGAAAAA ACGATGAAGT GCTGCCCTTT ACCGAGCATC TTCAATGCCT CACCAACACT 
TTACTCAGAC CAGACACCAG TTCTGTGCCA CTGGATGTAA CCCCCATCTT GAGCTACCTG 
TACGACAAGA AGTACTTGCC AAATATCGCA CTGGCATCTG CCAGGTTGTT TGTTCCCAGT 
GGCCTCAAGG GAGCACTGAC ACCCATTTGG AGAGCTGGCT TCATCCTCCA AAGTATTTCC 
AAACTCCTGG GAAGCCACCT GGAGAGGAGT AGCATCCCAG CGCTGGCTTG TTTCTACCTC 
AAGCAAGCCC TGCATTTCTC CTGCGAGACC AAAGCAGTGC CCACCCAGAG GACACTGTGT 
GCCATCTTGT CCAGGATGTA CCTCCAGCAC GGTGTCTTGG ATGGGGCGGT TTGTTACGCA 
GCCACAGCTG TAACCCTCAG CAGACGGATG GGCGAGGAGG AGGCTTTCGA GTCTTCCCTC 
TCCTTGGGGT GGATGTATCT CCTGAACAAC CGGCCGGGCC CAGCTGCAGA CATCATGTGG 
CAGCTCTTGC GCTCTCTACA CGAGACGGAC AGCGTGACTC AGGGTGGAGC AGTGCACAAT 
CTCCTGGCCA TCGCCCTCAA AGGAGAAGGA CAGGTGCAAA AGGCAGCAGA GAACTACCTT 
CGGGCCCTGC ACAAAGCCCA GGAGACTGGG AACAAGAGGA ACCAGGCTAT TGCCCTGGCT 
AACCTGGGGC AGCTGAGCTC CTCGCGCGGG GCGAGCCAGC TGTCTGAGCT CTACCTGCTC 
CAGTCGGCTC AGCTCTACGC TGAGCTCCAG GGCAGCGAAG ACCTGGAGAT GGAGTTGGTG 
CGGGTGTTGC TGTGGCTGGC ACAGGCCATG GTGAACAGGC TGAAGGTGGA AGATGGCAAA 
CTCTGTTATG AACTGGCGTT GGTTTTTGCC CTGAAGTGGC ATAACGTAAG GAGTCAGCTT 
CACGTCACTG AGTCTCTCTG CCATTTCTAC AGCAAGGTGT CCCCCAATCT CCAGGCCTGC 
ATTACCTACC ATGAGCACTG GGTATCATTG GCACAACAGC TCCAGGACAG AGAGATGGAA 
GGCAATGCCC GGCAGACCCT CAGCCAGCTC TACCAGGCTT TGGGCACATC TGAGGCTTTG 
AGACAGTCCC TGGACTGCAC CAAACAGAGT CTAAGGATCT TCATTGACCT TGAGGAGCCT 
GTGAAAGCAG CAGAGGCCTG GCTGCAGGCA GGAAGGCTCT ACTATCTTAT GCAGGAAGAT 
GAGCTGGTGG AAATGTATTT TCAGGCAGCC ATTCAGACTG CTCTGAAATG GGAGAACTTC 
TCCTTGGCCA TGGACCTTTA TGAAAAAGCA GGTGATACCT TTTTTAATGG CAACCGAAAC 
AGAGATCGAG CTGTGGAGTT TTATAGGGGC GGTGCTGTGC CCTTGGCCAG GAAACTGAAG 
TCCAGTAGGA CAGAGCTGCG GCTGTTCAAT AAGCTGGCAG AGCTGCAGAT CGGGCTGCAG 
GGCTACGAGA AGGCACTGGA GTTTGCCACA CTGGCAGCCA GGCTAAGCAT CAGGGTCGGA 
GATCAATTGC AAGAGCTGGT TGCATTCCAC CGCCTGGCGA CAGTCTACTA TTTTCTGCAT 
ATGTATGAGA TGGCAGAAGA CTGCTACCTG AAGACCCTTG CCTTGCGTCC CCCCTTGCTG 
CAGTGCTCTG GAGAGGCCCT GTACTACTGC AAGATGTATT GCCACCTTGG CAACCTGACC 
CTGCACAAAC TGAAGGATGA ACAGGATGCA GCAACCTACT TCCTCCTGGC CCTCGCCGCA 
GCAACCGAGC TGGGAGACCA GGAGCTGCAA GACTTCATTC ACGCCAAGCT GGGTGACATC 
CCCGGTGCCC CGTGGGGGCC CGAGGGCACG CCGGGCTGTG CCACATACCG GCCCAGGTGG 
CTGAGTGAAG GTGGCCACGT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014803183.1
CDS	536..4522
Translation	MLDNPQLGIPAGEECDSCCHRSPVLLPRWDLLQRTAAEPRGKWWWEKTMASGKAVVSEPSPPATEAAEGSPDTSSALLAVGEGFPPEISLFFSIESRSSQCLNPQLQEAARKKLWALESNDRDVCALFKELSARLVCMQAQEDRFLLTFKTLEEVWKFSTYLTLGYVGSCLEQLLFDQEYWLNCALMEDTEVRVTVDEDRLATIYMGLLLQEGSFFSRAVPGVWQPEQEGEEGLQLCKNELIHVKSVGEESKWEGMSLLTGQRGLVPVTALEPIPHPFYQWFLKNYAVSFGISQEISGTTSQPIVKGRCTATEDHRGAAPDELSFSKGDSIEVIGFLIPGLPWFVGKSVSSGSIGFVPTRYINPEACEPLGKGLVFLSEEEKSPLLRVPCNGGEQHFTTLLGDLAHTDITSVYRLDGFEPKAMFTKVPSEAVLHGCKDIQLLQSWEEINGLATTSTSELSSPGSESSPATLEDVLLEKMDDFDDPKFFIDLNAGHMEDADVFDPILTFLNQESYMPSFQSLYDLSFSFLNSTFYGFSDEDELVLYLETSRNWAKRTHSVWAHVRLCFLLGKLCIKKVKFSQARVYFEEAMSILDRGFGDLPLLAALHVNLASIYLKQNMKHKFSALMGKTVALLVCLPGHSFSSENELEVMMYVLKEAIAVGNAPLEARVCFLIVKLFLQLGKNDEVLPFTEHLQCLTNTLLRPDTSSVPLDVTPILSYLYDKKYLPNIALASARLFVPSGLKGALTPIWRAGFILQSISKLLGSHLERSSIPALACFYLKQALHFSCETKAVPTQRTLCAILSRMYLQHGVLDGAVCYAATAVTLSRRMGEEEAFESSLSLGWMYLLNNRPGPAADIMWQLLRSLHETDSVTQGGAVHNLLAIALKGEGQVQKAAENYLRALHKAQETGNKRNQAIALANLGQLSSSRGASQLSELYLLQSAQLYAELQGSEDLEMELVRVLLWLAQAMVNRLKVEDGKLCYELALVFALKWHNVRSQLHVTESLCHFYSKVSPNLQACITYHEHWVSLAQQLQDREMEGNARQTLSQLYQALGTSEALRQSLDCTKQSLRIFIDLEEPVKAAEAWLQAGRLYYLMQEDELVEMYFQAAIQTALKWENFSLAMDLYEKAGDTFFNGNRNRDRAVEFYRGGAVPLARKLKSSRTELRLFNKLAELQIGLQGYEKALEFATLAARLSIRVGDQLQELVAFHRLATVYYFLHMYEMAEDCYLKTLALRPPLLQCSGEALYYCKMYCHLGNLTLHKLKDEQDAATYFLLALAAATELGDQELQDFIHAKLGDIPGAPWGPEGTPGCATYRPRWLSEGGHVV

Target ORF information:

RefSeq Version	XM_014947697.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014947697.1

ORF Insert Sequence:

ATGCTGGACA ATCCCCAGCT GGGCATCCCT GCAGGCGAAG AGTGTGACTC CTGCTGCCAC 
CGCTCACCCG TGCTCCTGCC CCGCTGGGAC CTCCTGCAGC GCACCGCAGC CGAGCCCCGG 
GGAAAATGGT GGTGGGAGAA GACCATGGCA TCAGGTAAGG CTGTGGTATC AGAGCCAAGC 
CCCCCTGCTA CCGAGGCAGC AGAAGGCTCA CCGGACACCT CCTCGGCGCT GCTGGCTGTC 
GGGGAGGGCT TTCCACCAGA GATTTCGCTC TTCTTCTCCA TTGAGAGCCG CTCCTCACAG 
TGCCTCAATC CCCAGCTCCA GGAAGCAGCC AGAAAGAAGC TGTGGGCCCT GGAGAGTAAT 
GACAGAGACG TTTGTGCCCT GTTCAAGGAG CTGTCAGCCA GGCTGGTCTG TATGCAAGCA 
CAGGAGGACA GGTTCCTCCT CACCTTCAAA ACCCTGGAAG AAGTCTGGAA GTTTTCTACC 
TATCTGACTT TAGGGTACGT GGGCAGCTGC CTGGAACAGC TTCTCTTTGA CCAGGAATAC 
TGGCTAAACT GTGCTCTGAT GGAAGACACA GAGGTCAGAG TTACTGTGGA TGAAGATCGC 
TTGGCCACCA TATATATGGG TCTGCTGCTC CAAGAAGGTA GCTTTTTTTC CAGAGCAGTG 
CCCGGTGTCT GGCAGCCAGA GCAGGAGGGA GAGGAAGGTC TGCAGCTCTG CAAGAACGAG 
CTGATCCATG TGAAGAGCGT TGGAGAGGAG TCCAAGTGGG AAGGGATGTC CTTACTGACA 
GGTCAACGAG GCCTTGTGCC TGTGACAGCT CTAGAGCCAA TACCTCATCC ATTTTACCAG 
TGGTTCCTGA AGAATTATGC TGTGAGTTTT GGCATCTCCC AAGAGATCAG TGGGACAACT 
TCTCAGCCAA TTGTCAAAGG CAGGTGCACA GCCACAGAGG ACCACAGAGG AGCAGCACCG 
GATGAACTGA GTTTCTCCAA AGGAGACAGC ATAGAAGTCA TCGGCTTCCT CATTCCAGGA 
CTCCCATGGT TTGTGGGCAA ATCCGTCAGC AGTGGGAGCA TTGGCTTTGT CCCAACCCGA 
TACATAAATC CTGAGGCTTG TGAACCTCTG GGAAAAGGCT TGGTGTTTCT GAGTGAAGAA 
GAAAAATCCC CGCTCCTGCG AGTCCCCTGT AATGGTGGGG AACAGCACTT CACCACCCTC 
CTCGGTGACC TGGCACACAC CGACATCACC TCTGTGTACC GCCTGGATGG TTTTGAACCT 
AAAGCCATGT TCACAAAAGT GCCATCAGAG GCTGTTCTCC ATGGCTGTAA AGATATCCAG 
CTGCTCCAAT CTTGGGAGGA AATAAATGGC TTGGCTACAA CTAGCACCTC CGAGCTGTCT 
AGCCCAGGGA GTGAATCCTC CCCTGCTACA TTGGAAGATG TTCTCCTGGA GAAGATGGAT 
GATTTTGATG ATCCTAAGTT CTTTATTGAC CTGAACGCTG GACACATGGA AGATGCTGAT 
GTCTTTGACC CCATATTGAC CTTCCTTAAC CAAGAGAGTT ATATGCCCAG TTTCCAAAGC 
CTCTATGATC TCAGTTTTTC CTTTCTCAAC TCCACTTTTT ATGGTTTCTC TGATGAGGAT 
GAACTGGTCT TGTACCTTGA GACATCCAGG AACTGGGCCA AGAGGACTCA TTCCGTTTGG 
GCTCATGTCA GGCTCTGTTT CCTCTTGGGT AAGCTCTGCA TCAAAAAGGT CAAGTTCTCC 
CAGGCTCGGG TCTACTTTGA GGAAGCCATG AGCATCCTGG ATAGGGGTTT TGGGGATCTG 
CCCCTCTTGG CTGCATTGCA TGTGAACCTT GCCTCAATCT ACTTGAAACA AAATATGAAG 
CACAAGTTCT CCGCCTTGAT GGGGAAAACG GTGGCCTTGC TTGTCTGCTT GCCTGGCCAC 
TCTTTCAGCT CTGAGAATGA GCTGGAAGTC ATGATGTATG TCCTGAAGGA AGCTATTGCT 
GTGGGTAATG CTCCCTTGGA GGCACGGGTC TGCTTCCTTA TTGTCAAGCT CTTCCTACAA 
CTGGGAAAAA ACGATGAAGT GCTGCCCTTT ACCGAGCATC TTCAATGCCT CACCAACACT 
TTACTCAGAC CAGACACCAG TTCTGTGCCA CTGGATGTAA CCCCCATCTT GAGCTACCTG 
TACGACAAGA AGTACTTGCC AAATATCGCA CTGGCATCTG CCAGGTTGTT TGTTCCCAGT 
GGCCTCAAGG GAGCACTGAC ACCCATTTGG AGAGCTGGCT TCATCCTCCA AAGTATTTCC 
AAACTCCTGG GAAGCCACCT GGAGAGGAGT AGCATCCCAG CGCTGGCTTG TTTCTACCTC 
AAGCAAGCCC TGCATTTCTC CTGCGAGACC AAAGCAGTGC CCACCCAGAG GACACTGTGT 
GCCATCTTGT CCAGGATGTA CCTCCAGCAC GGTGTCTTGG ATGGGGCGGT TTGTTACGCA 
GCCACAGCTG TAACCCTCAG CAGACGGATG GGCGAGGAGG AGGCTTTCGA GTCTTCCCTC 
TCCTTGGGGT GGATGTATCT CCTGAACAAC CGGCCGGGCC CAGCTGCAGA CATCATGTGG 
CAGCTCTTGC GCTCTCTACA CGAGACGGAC AGCGTGACTC AGGGTGGAGC AGTGCACAAT 
CTCCTGGCCA TCGCCCTCAA AGGAGAAGGA CAGGTGCAAA AGGCAGCAGA GAACTACCTT 
CGGGCCCTGC ACAAAGCCCA GGAGACTGGG AACAAGAGGA ACCAGGCTAT TGCCCTGGCT 
AACCTGGGGC AGCTGAGCTC CTCGCGCGGG GCGAGCCAGC TGTCTGAGCT CTACCTGCTC 
CAGTCGGCTC AGCTCTACGC TGAGCTCCAG GGCAGCGAAG ACCTGGAGAT GGAGTTGGTG 
CGGGTGTTGC TGTGGCTGGC ACAGGCCATG GTGAACAGGC TGAAGGTGGA AGATGGCAAA 
CTCTGTTATG AACTGGCGTT GGTTTTTGCC CTGAAGTGGC ATAACGTAAG GAGTCAGCTT 
CACGTCACTG AGTCTCTCTG CCATTTCTAC AGCAAGGTGT CCCCCAATCT CCAGGCCTGC 
ATTACCTACC ATGAGCACTG GGTATCATTG GCACAACAGC TCCAGGACAG AGAGATGGAA 
GGCAATGCCC GGCAGACCCT CAGCCAGCTC TACCAGGCTT TGGGCACATC TGAGGCTTTG 
AGACAGTCCC TGGACTGCAC CAAACAGAGT CTAAGGATCT TCATTGACCT TGAGGAGCCT 
GTGAAAGCAG CAGAGGCCTG GCTGCAGGCA GGAAGGCTCT ACTATCTTAT GCAGGAAGAT 
GAGCTGGTGG AAATGTATTT TCAGGCAGCC ATTCAGACTG CTCTGAAATG GGAGAACTTC 
TCCTTGGCCA TGGACCTTTA TGAAAAAGCA GGTGATACCT TTTTTAATGG CAACCGAAAC 
AGAGATCGAG CTGTGGAGTT TTATAGGGGC GGTGCTGTGC CCTTGGCCAG GAAACTGAAG 
TCCAGTAGGA CAGAGCTGCG GCTGTTCAAT AAGCTGGCAG AGCTGCAGAT CGGGCTGCAG 
GGCTACGAGA AGGCACTGGA GTTTGCCACA CTGGCAGCCA GGCTAAGCAT CAGGGTCGGA 
GATCAATTGC AAGAGCTGGT TGCATTCCAC CGCCTGGCGA CAGTCTACTA TTTTCTGCAT 
ATGTATGAGA TGGCAGAAGA CTGCTACCTG AAGACCCTTG CCTTGCGTCC CCCCTTGCTG 
CAGTGCTCTG GAGAGGCCCT GTACTACTGC AAGATGTATT GCCACCTTGG CAACCTGACC 
CTGCACAAAC TGAAGGATGA ACAGGATGCA GCAACCTACT TCCTCCTGGC CCTCGCCGCA 
GCAACCGAGC TGGGAGACCA GGAGCTGCAA GACTTCATTC ACGCCAAGCT GGGTGACATC 
CCCGGTGCCC CGTGGGGGCC CGAGGGCACG CCGGGCTGTG CCACATACCG GCCCAGGTGG 
CTGAGTGAAG GTGGCCACGT TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3TC2 ( XM_014947698.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014803184.1 Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCa69214
Clone ID Related Accession (Same CDS sequence)	XM_014947698.1
Accession Version	XM_014947698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAG GTAAGGCTGT GGTATCAGAG CCAAGCCCCC CTGCTACCGA GGCAGCAGAA 
GGCTCACCGG ACACCTCCTC GGCGCTGCTG GCTGTCGGGG AGGGCTTTCC ACCAGAGATT 
TCGCTCTTCT TCTCCATTGA GAGCCGCTCC TCACAGTGCC TCAATCCCCA GCTCCAGGAA 
GCAGCCAGAA AGAAGCTGTG GGCCCTGGAG AGTAATGACA GAGACGTTTG TGCCCTGTTC 
AAGGAGCTGT CAGCCAGGCT GGTCTGTATG CAAGCACAGG AGGACAGGTT CCTCCTCACC 
TTCAAAACCC TGGAAGAAGT CTGGAAGTTT TCTACCTATC TGACTTTAGG GTACGTGGGC 
AGCTGCCTGG AACAGCTTCT CTTTGACCAG GAATACTGGC TAAACTGTGC TCTGATGGAA 
GACACAGAGG TCAGAGTTAC TGTGGATGAA GATCGCTTGG CCACCATATA TATGGGTCTG 
CTGCTCCAAG AAGGTAGCTT TTTTTCCAGA GCAGTGCCCG GTGTCTGGCA GCCAGAGCAG 
GAGGGAGAGG AAGGTCTGCA GCTCTGCAAG AACGAGCTGA TCCATGTGAA GAGCGTTGGA 
GAGGAGTCCA AGTGGGAAGG GATGTCCTTA CTGACAGGTC AACGAGGCCT TGTGCCTGTG 
ACAGCTCTAG AGCCAATACC TCATCCATTT TACCAGTGGT TCCTGAAGAA TTATGCTGTG 
AGTTTTGGCA TCTCCCAAGA GATCAGTGGG ACAACTTCTC AGCCAATTGT CAAAGGCAGG 
TGCACAGCCA CAGAGGACCA CAGAGGAGCA GCACCGGATG AACTGAGTTT CTCCAAAGGA 
GACAGCATAG AAGTCATCGG CTTCCTCATT CCAGGACTCC CATGGTTTGT GGGCAAATCC 
GTCAGCAGTG GGAGCATTGG CTTTGTCCCA ACCCGATACA TAAATCCTGA GGCTTGTGAA 
CCTCTGGGAA AAGGCTTGGT GTTTCTGAGT GAAGAAGAAA AATCCCCGCT CCTGCGAGTC 
CCCTGTAATG GTGGGGAACA GCACTTCACC ACCCTCCTCG GTGACCTGGC ACACACCGAC 
ATCACCTCTG TGTACCGCCT GGATGGTTTT GAACCTAAAG CCATGTTCAC AAAAGTGCCA 
TCAGAGGCTG TTCTCCATGG CTGTAAAGAT ATCCAGCTGC TCCAATCTTG GGAGGAAATA 
AATGGCTTGG CTACAACTAG CACCTCCGAG CTGTCTAGCC CAGGGAGTGA ATCCTCCCCT 
GCTACATTGG AAGATGTTCT CCTGGAGAAG ATGGATGATT TTGATGATCC TAAGTTCTTT 
ATTGACCTGA ACGCTGGACA CATGGAAGAT GCTGATGTCT TTGACCCCAT ATTGACCTTC 
CTTAACCAAG AGAGTTATAT GCCCAGTTTC CAAAGCCTCT ATGATCTCAG TTTTTCCTTT 
CTCAACTCCA CTTTTTATGG TTTCTCTGAT GAGGATGAAC TGGTCTTGTA CCTTGAGACA 
TCCAGGAACT GGGCCAAGAG GACTCATTCC GTTTGGGCTC ATGTCAGGCT CTGTTTCCTC 
TTGGGTAAGC TCTGCATCAA AAAGGTCAAG TTCTCCCAGG CTCGGGTCTA CTTTGAGGAA 
GCCATGAGCA TCCTGGATAG GGGTTTTGGG GATCTGCCCC TCTTGGCTGC ATTGCATGTG 
AACCTTGCCT CAATCTACTT GAAACAAAAT ATGAAGCACA AGTTCTCCGC CTTGATGGGG 
AAAACGGTGG CCTTGCTTGT CTGCTTGCCT GGCCACTCTT TCAGCTCTGA GAATGAGCTG 
GAAGTCATGA TGTATGTCCT GAAGGAAGCT ATTGCTGTGG GTAATGCTCC CTTGGAGGCA 
CGGGTCTGCT TCCTTATTGT CAAGCTCTTC CTACAACTGG GAAAAAACGA TGAAGTGCTG 
CCCTTTACCG AGCATCTTCA ATGCCTCACC AACACTTTAC TCAGACCAGA CACCAGTTCT 
GTGCCACTGG ATGTAACCCC CATCTTGAGC TACCTGTACG ACAAGAAGTA CTTGCCAAAT 
ATCGCACTGG CATCTGCCAG GTTGTTTGTT CCCAGTGGCC TCAAGGGAGC ACTGACACCC 
ATTTGGAGAG CTGGCTTCAT CCTCCAAAGT ATTTCCAAAC TCCTGGGAAG CCACCTGGAG 
AGGAGTAGCA TCCCAGCGCT GGCTTGTTTC TACCTCAAGC AAGCCCTGCA TTTCTCCTGC 
GAGACCAAAG CAGTGCCCAC CCAGAGGACA CTGTGTGCCA TCTTGTCCAG GATGTACCTC 
CAGCACGGTG TCTTGGATGG GGCGGTTTGT TACGCAGCCA CAGCTGTAAC CCTCAGCAGA 
CGGATGGGCG AGGAGGAGGC TTTCGAGTCT TCCCTCTCCT TGGGGTGGAT GTATCTCCTG 
AACAACCGGC CGGGCCCAGC TGCAGACATC ATGTGGCAGC TCTTGCGCTC TCTACACGAG 
ACGGACAGCG TGACTCAGGG TGGAGCAGTG CACAATCTCC TGGCCATCGC CCTCAAAGGA 
GAAGGACAGG TGCAAAAGGC AGCAGAGAAC TACCTTCGGG CCCTGCACAA AGCCCAGGAG 
ACTGGGAACA AGAGGAACCA GGCTATTGCC CTGGCTAACC TGGGGCAGCT GAGCTCCTCG 
CGCGGGGCGA GCCAGCTGTC TGAGCTCTAC CTGCTCCAGT CGGCTCAGCT CTACGCTGAG 
CTCCAGGGCA GCGAAGACCT GGAGATGGAG TTGGTGCGGG TGTTGCTGTG GCTGGCACAG 
GCCATGGTGA ACAGGCTGAA GGTGGAAGAT GGCAAACTCT GTTATGAACT GGCGTTGGTT 
TTTGCCCTGA AGTGGCATAA CGTAAGGAGT CAGCTTCACG TCACTGAGTC TCTCTGCCAT 
TTCTACAGCA AGGTGTCCCC CAATCTCCAG GCCTGCATTA CCTACCATGA GCACTGGGTA 
TCATTGGCAC AACAGCTCCA GGACAGAGAG ATGGAAGGCA ATGCCCGGCA GACCCTCAGC 
CAGCTCTACC AGGCTTTGGG CACATCTGAG GCTTTGAGAC AGTCCCTGGA CTGCACCAAA 
CAGAGTCTAA GGATCTTCAT TGACCTTGAG GAGCCTGTGA AAGCAGCAGA GGCCTGGCTG 
CAGGCAGGAA GGCTCTACTA TCTTATGCAG GAAGATGAGC TGGTGGAAAT GTATTTTCAG 
GCAGCCATTC AGACTGCTCT GAAATGGGAG AACTTCTCCT TGGCCATGGA CCTTTATGAA 
AAAGCAGGTG ATACCTTTTT TAATGGCAAC CGAAACAGAG ATCGAGCTGT GGAGTTTTAT 
AGGGGCGGTG CTGTGCCCTT GGCCAGGAAA CTGAAGTCCA GTAGGACAGA GCTGCGGCTG 
TTCAATAAGC TGGCAGAGCT GCAGATCGGG CTGCAGGGCT ACGAGAAGGC ACTGGAGTTT 
GCCACACTGG CAGCCAGGCT AAGCATCAGG GTCGGAGATC AATTGCAAGA GCTGGTTGCA 
TTCCACCGCC TGGCGACAGT CTACTATTTT CTGCATATGT ATGAGATGGC AGAAGACTGC 
TACCTGAAGA CCCTTGCCTT GCGTCCCCCC TTGCTGCAGT GCTCTGGAGA GGCCCTGTAC 
TACTGCAAGA TGTATTGCCA CCTTGGCAAC CTGACCCTGC ACAAACTGAA GGATGAACAG 
GATGCAGCAA CCTACTTCCT CCTGGCCCTC GCCGCAGCAA CCGAGCTGGG AGACCAGGAG 
CTGCAAGACT TCATTCACGC CAAGCTGGGT GACATCCCCG GTGCCCCGTG GGGGCCCGAG 
GGCACGCCGG GCTGTGCCAC ATACCGGCCC AGGTGGCTGA GTGAAGGTGG CCACGTTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014803184.1
CDS	353..4195
Translation	MASGKAVVSEPSPPATEAAEGSPDTSSALLAVGEGFPPEISLFFSIESRSSQCLNPQLQEAARKKLWALESNDRDVCALFKELSARLVCMQAQEDRFLLTFKTLEEVWKFSTYLTLGYVGSCLEQLLFDQEYWLNCALMEDTEVRVTVDEDRLATIYMGLLLQEGSFFSRAVPGVWQPEQEGEEGLQLCKNELIHVKSVGEESKWEGMSLLTGQRGLVPVTALEPIPHPFYQWFLKNYAVSFGISQEISGTTSQPIVKGRCTATEDHRGAAPDELSFSKGDSIEVIGFLIPGLPWFVGKSVSSGSIGFVPTRYINPEACEPLGKGLVFLSEEEKSPLLRVPCNGGEQHFTTLLGDLAHTDITSVYRLDGFEPKAMFTKVPSEAVLHGCKDIQLLQSWEEINGLATTSTSELSSPGSESSPATLEDVLLEKMDDFDDPKFFIDLNAGHMEDADVFDPILTFLNQESYMPSFQSLYDLSFSFLNSTFYGFSDEDELVLYLETSRNWAKRTHSVWAHVRLCFLLGKLCIKKVKFSQARVYFEEAMSILDRGFGDLPLLAALHVNLASIYLKQNMKHKFSALMGKTVALLVCLPGHSFSSENELEVMMYVLKEAIAVGNAPLEARVCFLIVKLFLQLGKNDEVLPFTEHLQCLTNTLLRPDTSSVPLDVTPILSYLYDKKYLPNIALASARLFVPSGLKGALTPIWRAGFILQSISKLLGSHLERSSIPALACFYLKQALHFSCETKAVPTQRTLCAILSRMYLQHGVLDGAVCYAATAVTLSRRMGEEEAFESSLSLGWMYLLNNRPGPAADIMWQLLRSLHETDSVTQGGAVHNLLAIALKGEGQVQKAAENYLRALHKAQETGNKRNQAIALANLGQLSSSRGASQLSELYLLQSAQLYAELQGSEDLEMELVRVLLWLAQAMVNRLKVEDGKLCYELALVFALKWHNVRSQLHVTESLCHFYSKVSPNLQACITYHEHWVSLAQQLQDREMEGNARQTLSQLYQALGTSEALRQSLDCTKQSLRIFIDLEEPVKAAEAWLQAGRLYYLMQEDELVEMYFQAAIQTALKWENFSLAMDLYEKAGDTFFNGNRNRDRAVEFYRGGAVPLARKLKSSRTELRLFNKLAELQIGLQGYEKALEFATLAARLSIRVGDQLQELVAFHRLATVYYFLHMYEMAEDCYLKTLALRPPLLQCSGEALYYCKMYCHLGNLTLHKLKDEQDAATYFLLALAAATELGDQELQDFIHAKLGDIPGAPWGPEGTPGCATYRPRWLSEGGHVV

Target ORF information:

RefSeq Version	XM_014947698.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014947698.1

ORF Insert Sequence:

ATGGCATCAG GTAAGGCTGT GGTATCAGAG CCAAGCCCCC CTGCTACCGA GGCAGCAGAA 
GGCTCACCGG ACACCTCCTC GGCGCTGCTG GCTGTCGGGG AGGGCTTTCC ACCAGAGATT 
TCGCTCTTCT TCTCCATTGA GAGCCGCTCC TCACAGTGCC TCAATCCCCA GCTCCAGGAA 
GCAGCCAGAA AGAAGCTGTG GGCCCTGGAG AGTAATGACA GAGACGTTTG TGCCCTGTTC 
AAGGAGCTGT CAGCCAGGCT GGTCTGTATG CAAGCACAGG AGGACAGGTT CCTCCTCACC 
TTCAAAACCC TGGAAGAAGT CTGGAAGTTT TCTACCTATC TGACTTTAGG GTACGTGGGC 
AGCTGCCTGG AACAGCTTCT CTTTGACCAG GAATACTGGC TAAACTGTGC TCTGATGGAA 
GACACAGAGG TCAGAGTTAC TGTGGATGAA GATCGCTTGG CCACCATATA TATGGGTCTG 
CTGCTCCAAG AAGGTAGCTT TTTTTCCAGA GCAGTGCCCG GTGTCTGGCA GCCAGAGCAG 
GAGGGAGAGG AAGGTCTGCA GCTCTGCAAG AACGAGCTGA TCCATGTGAA GAGCGTTGGA 
GAGGAGTCCA AGTGGGAAGG GATGTCCTTA CTGACAGGTC AACGAGGCCT TGTGCCTGTG 
ACAGCTCTAG AGCCAATACC TCATCCATTT TACCAGTGGT TCCTGAAGAA TTATGCTGTG 
AGTTTTGGCA TCTCCCAAGA GATCAGTGGG ACAACTTCTC AGCCAATTGT CAAAGGCAGG 
TGCACAGCCA CAGAGGACCA CAGAGGAGCA GCACCGGATG AACTGAGTTT CTCCAAAGGA 
GACAGCATAG AAGTCATCGG CTTCCTCATT CCAGGACTCC CATGGTTTGT GGGCAAATCC 
GTCAGCAGTG GGAGCATTGG CTTTGTCCCA ACCCGATACA TAAATCCTGA GGCTTGTGAA 
CCTCTGGGAA AAGGCTTGGT GTTTCTGAGT GAAGAAGAAA AATCCCCGCT CCTGCGAGTC 
CCCTGTAATG GTGGGGAACA GCACTTCACC ACCCTCCTCG GTGACCTGGC ACACACCGAC 
ATCACCTCTG TGTACCGCCT GGATGGTTTT GAACCTAAAG CCATGTTCAC AAAAGTGCCA 
TCAGAGGCTG TTCTCCATGG CTGTAAAGAT ATCCAGCTGC TCCAATCTTG GGAGGAAATA 
AATGGCTTGG CTACAACTAG CACCTCCGAG CTGTCTAGCC CAGGGAGTGA ATCCTCCCCT 
GCTACATTGG AAGATGTTCT CCTGGAGAAG ATGGATGATT TTGATGATCC TAAGTTCTTT 
ATTGACCTGA ACGCTGGACA CATGGAAGAT GCTGATGTCT TTGACCCCAT ATTGACCTTC 
CTTAACCAAG AGAGTTATAT GCCCAGTTTC CAAAGCCTCT ATGATCTCAG TTTTTCCTTT 
CTCAACTCCA CTTTTTATGG TTTCTCTGAT GAGGATGAAC TGGTCTTGTA CCTTGAGACA 
TCCAGGAACT GGGCCAAGAG GACTCATTCC GTTTGGGCTC ATGTCAGGCT CTGTTTCCTC 
TTGGGTAAGC TCTGCATCAA AAAGGTCAAG TTCTCCCAGG CTCGGGTCTA CTTTGAGGAA 
GCCATGAGCA TCCTGGATAG GGGTTTTGGG GATCTGCCCC TCTTGGCTGC ATTGCATGTG 
AACCTTGCCT CAATCTACTT GAAACAAAAT ATGAAGCACA AGTTCTCCGC CTTGATGGGG 
AAAACGGTGG CCTTGCTTGT CTGCTTGCCT GGCCACTCTT TCAGCTCTGA GAATGAGCTG 
GAAGTCATGA TGTATGTCCT GAAGGAAGCT ATTGCTGTGG GTAATGCTCC CTTGGAGGCA 
CGGGTCTGCT TCCTTATTGT CAAGCTCTTC CTACAACTGG GAAAAAACGA TGAAGTGCTG 
CCCTTTACCG AGCATCTTCA ATGCCTCACC AACACTTTAC TCAGACCAGA CACCAGTTCT 
GTGCCACTGG ATGTAACCCC CATCTTGAGC TACCTGTACG ACAAGAAGTA CTTGCCAAAT 
ATCGCACTGG CATCTGCCAG GTTGTTTGTT CCCAGTGGCC TCAAGGGAGC ACTGACACCC 
ATTTGGAGAG CTGGCTTCAT CCTCCAAAGT ATTTCCAAAC TCCTGGGAAG CCACCTGGAG 
AGGAGTAGCA TCCCAGCGCT GGCTTGTTTC TACCTCAAGC AAGCCCTGCA TTTCTCCTGC 
GAGACCAAAG CAGTGCCCAC CCAGAGGACA CTGTGTGCCA TCTTGTCCAG GATGTACCTC 
CAGCACGGTG TCTTGGATGG GGCGGTTTGT TACGCAGCCA CAGCTGTAAC CCTCAGCAGA 
CGGATGGGCG AGGAGGAGGC TTTCGAGTCT TCCCTCTCCT TGGGGTGGAT GTATCTCCTG 
AACAACCGGC CGGGCCCAGC TGCAGACATC ATGTGGCAGC TCTTGCGCTC TCTACACGAG 
ACGGACAGCG TGACTCAGGG TGGAGCAGTG CACAATCTCC TGGCCATCGC CCTCAAAGGA 
GAAGGACAGG TGCAAAAGGC AGCAGAGAAC TACCTTCGGG CCCTGCACAA AGCCCAGGAG 
ACTGGGAACA AGAGGAACCA GGCTATTGCC CTGGCTAACC TGGGGCAGCT GAGCTCCTCG 
CGCGGGGCGA GCCAGCTGTC TGAGCTCTAC CTGCTCCAGT CGGCTCAGCT CTACGCTGAG 
CTCCAGGGCA GCGAAGACCT GGAGATGGAG TTGGTGCGGG TGTTGCTGTG GCTGGCACAG 
GCCATGGTGA ACAGGCTGAA GGTGGAAGAT GGCAAACTCT GTTATGAACT GGCGTTGGTT 
TTTGCCCTGA AGTGGCATAA CGTAAGGAGT CAGCTTCACG TCACTGAGTC TCTCTGCCAT 
TTCTACAGCA AGGTGTCCCC CAATCTCCAG GCCTGCATTA CCTACCATGA GCACTGGGTA 
TCATTGGCAC AACAGCTCCA GGACAGAGAG ATGGAAGGCA ATGCCCGGCA GACCCTCAGC 
CAGCTCTACC AGGCTTTGGG CACATCTGAG GCTTTGAGAC AGTCCCTGGA CTGCACCAAA 
CAGAGTCTAA GGATCTTCAT TGACCTTGAG GAGCCTGTGA AAGCAGCAGA GGCCTGGCTG 
CAGGCAGGAA GGCTCTACTA TCTTATGCAG GAAGATGAGC TGGTGGAAAT GTATTTTCAG 
GCAGCCATTC AGACTGCTCT GAAATGGGAG AACTTCTCCT TGGCCATGGA CCTTTATGAA 
AAAGCAGGTG ATACCTTTTT TAATGGCAAC CGAAACAGAG ATCGAGCTGT GGAGTTTTAT 
AGGGGCGGTG CTGTGCCCTT GGCCAGGAAA CTGAAGTCCA GTAGGACAGA GCTGCGGCTG 
TTCAATAAGC TGGCAGAGCT GCAGATCGGG CTGCAGGGCT ACGAGAAGGC ACTGGAGTTT 
GCCACACTGG CAGCCAGGCT AAGCATCAGG GTCGGAGATC AATTGCAAGA GCTGGTTGCA 
TTCCACCGCC TGGCGACAGT CTACTATTTT CTGCATATGT ATGAGATGGC AGAAGACTGC 
TACCTGAAGA CCCTTGCCTT GCGTCCCCCC TTGCTGCAGT GCTCTGGAGA GGCCCTGTAC 
TACTGCAAGA TGTATTGCCA CCTTGGCAAC CTGACCCTGC ACAAACTGAA GGATGAACAG 
GATGCAGCAA CCTACTTCCT CCTGGCCCTC GCCGCAGCAA CCGAGCTGGG AGACCAGGAG 
CTGCAAGACT TCATTCACGC CAAGCTGGGT GACATCCCCG GTGCCCCGTG GGGGCCCGAG 
GGCACGCCGG GCTGTGCCAC ATACCGGCCC AGGTGGCTGA GTGAAGGTGG CCACGTTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3TC2 ( XM_014947699.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014803185.1 Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X8, mRNA.

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CloneID	OCa69215
Clone ID Related Accession (Same CDS sequence)	XM_014947699.1
Accession Version	XM_014947699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	SH3 domain and tetratricopeptide repeat-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCT GCTTGGGGAC CCCCTGGCAC CAGGACCTGA CCCTGGGGTG CACAGGCGAA 
GAGTGTGACT CCTGCTGCCA CCGCTCACCC GTGCTCCTGC CCCGCTGGGA CCTCCTGCAG 
CGCACCGCAG CCGAGCCCCG GGGAAAATGG TGGTGGGAGA AGACCATGGC ATCAGGTAAG 
GCTGTGGTAT CAGAGCCAAG CCCCCCTGCT ACCGAGGCAG CAGAAGGCTC ACCGGACACC 
TCCTCGGCGC TGCTGGCTGT CGGGGAGGGC TTTCCACCAG AGATTTCGCT CTTCTTCTCC 
ATTGAGAGCC GCTCCTCACA GTGCCTCAAT CCCCAGCTCC AGGAAGCAGC CAGAAAGAAG 
CTGTGGGCCC TGGAGAGTAA TGACAGAGAC GTTTGTGCCC TGTTCAAGGA GCTGTCAGCC 
AGGCTGGTCT GTATGCAAGC ACAGGAGGAC AGGTTCCTCC TCACCTTCAA AACCCTGGAA 
GAAGTCTGGA AGTTTTCTAC CTATCTGACT TTAGGGTACG TGGGCAGCTG CCTGGAACAG 
CTTCTCTTTG ACCAGGAATA CTGGCTAAAC TGTGCTCTGA TGGAAGACAC AGAGGTCAGA 
GTTACTGTGG ATGAAGATCG CTTGGCCACC ATATATATGG GTCTGCTGCT CCAAGAAGGT 
AGCTTTTTTT CCAGAGCAGT GCCCGGTGTC TGGCAGCCAG AGCAGGAGGG AGAGGAAGGT 
CTGCAGCTCT GCAAGAACGA GCTGATCCAT GTGAAGAGCG TTGGAGAGGA GTCCAAGTGG 
GAAGGGATGT CCTTACTGAC AGGTCAACGA GGCCTTGTGC CTGTGACAGC TCTAGAGCCA 
ATACCTCATC CATTTTACCA GTGGTTCCTG AAGAATTATG CTGTGAGTTT TGGCATCTCC 
CAAGAGATCA GTGGGACAAC TTCTCAGCCA ATTGTCAAAG GCAGGTGCAC AGCCACAGAG 
GACCACAGAG GAGCAGCACC GGATGAACTG AGTTTCTCCA AAGGAGACAG CATAGAAGTC 
ATCGGCTTCC TCATTCCAGG ACTCCCATGG TTTGTGGGCA AATCCGTCAG CAGTGGGAGC 
ATTGGCTTTG TCCCAACCCG ATACATAAAT CCTGAGGCTT GTGAACCTCT GGGAAAAGGC 
TTGGTGTTTC TGAGTGAAGA AGAAAAATCC CCGCTCCTGC GAGTCCCCTG TAATGGTGGG 
GAACAGCACT TCACCACCCT CCTCGGTGAC CTGGCACACA CCGACATCAC CTCTGTGTAC 
CGCCTGGATG GTTTTGAACC TAAAGCCATG TTCACAAAAG TGCCATCAGA GGCTGTTCTC 
CATGGCTGTA AAGATATCCA GCTGCTCCAA TCTTGGGAGG AAATAAATGG CTTGGCTACA 
ACTAGCACCT CCGAGCTGTC TAGCCCAGGG AGTGAATCCT CCCCTGCTAC ATTGGAAGAT 
GTTCTCCTGG AGAAGATGGA TGATTTTGAT GATCCTAAGT TCTTTATTGA CCTGAACGCT 
GGACACATGG AAGATGCTGA TGTCTTTGAC CCCATATTGA CCTTCCTTAA CCAAGAGAGT 
TATATGCCCA GTTTCCAAAG CCTCTATGAT CTCAGTTTTT CCTTTCTCAA CTCCACTTTT 
TATGGTTTCT CTGATGAGGA TGAACTGGTC TTGTACCTTG AGACATCCAG GAACTGGGCC 
AAGAGGACTC ATTCCGTTTG GGCTCATGTC AGGCTCTGTT TCCTCTTGGG TAAGCTCTGC 
ATCAAAAAGG TCAAGTTCTC CCAGGCTCGG GTCTACTTTG AGGAAGCCAT GAGCATCCTG 
GATAGGGGTT TTGGGGATCT GCCCCTCTTG GCTGCATTGC ATGTGAACCT TGCCTCAATC 
TACTTGAAAC AAAATATGAA GCACAAGTTC TCCGCCTTGA TGGGGAAAAC GGTGGCCTTG 
CTTGTCTGCT TGCCTGGCCA CTCTTTCAGC TCTGAGAATG AGCTGGAAGT CATGATGTAT 
GTCCTGAAGG AAGCTATTGC TGTGGGTAAT GCTCCCTTGG AGGCACGGGT CTGCTTCCTT 
ATTGTCAAGC TCTTCCTACA ACTGGGAAAA AACGATGAAG TGCTGCCCTT TACCGAGCAT 
CTTCAATGCC TCACCAACAC TTTACTCAGA CCAGACACCA GTTCTGTGCC ACTGGATGTA 
ACCCCCATCT TGAGCTACCT GTACGACAAG AAGTACTTGC CAAATATCGC ACTGGCATCT 
GCCAGGTTGT TTGTTCCCAG TGGCCTCAAG GGAGCACTGA CACCCATTTG GAGAGCTGGC 
TTCATCCTCC AAAGTATTTC CAAACTCCTG GGAAGCCACC TGGAGAGGAG TAGCATCCCA 
GCGCTGGCTT GTTTCTACCT CAAGCAAGCC CTGCATTTCT CCTGCGAGAC CAAAGCAGTG 
CCCACCCAGA GGACACTGTG TGCCATCTTG TCCAGGATGT ACCTCCAGCA CGGTGTCTTG 
GATGGGGCGG TTTGTTACGC AGCCACAGCT GTAACCCTCA GCAGACGGAT GGGCGAGGAG 
GAGGCTTTCG AGTCTTCCCT CTCCTTGGGG TGGATGTATC TCCTGAACAA CCGGCCGGGC 
CCAGCTGCAG ACATCATGTG GCAGCTCTTG CGCTCTCTAC ACGAGACGGA CAGCGTGACT 
CAGGGTGGAG CAGTGCACAA TCTCCTGGCC ATCGCCCTCA AAGGAGAAGG ACAGGTGCAA 
AAGGCAGCAG AGAACTACCT TCGGGCCCTG CACAAAGCCC AGGAGACTGG GAACAAGAGG 
AACCAGGCTA TTGCCCTGGC TAACCTGGGG CAGCTGAGCT CCTCGCGCGG GGCGAGCCAG 
CTGTCTGAGC TCTACCTGCT CCAGTCGGCT CAGCTCTACG CTGAGCTCCA GGGCAGCGAA 
GACCTGGAGA TGGAGTTGGT GCGGGTGTTG CTGTGGCTGG CACAGGCCAT GGTGAACAGG 
CTGAAGGTGG AAGATGGCAA ACTCTGTTAT GAACTGGCGT TGGTTTTTGC CCTGAAGTGG 
CATAACGTCA GCTTCACGTC ACTGAGTCTC TCTGCCATTT CTACAGCAAG GTGTCCCCCA 
ATCTCCAGGC CTGCATTACC TACCATGAGC ACTGGGTATC ATTGGCACAA CAGCTCCAGG 
ACAGAGAGAT GGAAGGCAAT GCCCGGCAGA CCCTCAGCCA GCTCTACCAG GCTTTGGGCA 
CATCTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014803185.1
CDS	20..3214
Translation	MGCCLGTPWHQDLTLGCTGEECDSCCHRSPVLLPRWDLLQRTAAEPRGKWWWEKTMASGKAVVSEPSPPATEAAEGSPDTSSALLAVGEGFPPEISLFFSIESRSSQCLNPQLQEAARKKLWALESNDRDVCALFKELSARLVCMQAQEDRFLLTFKTLEEVWKFSTYLTLGYVGSCLEQLLFDQEYWLNCALMEDTEVRVTVDEDRLATIYMGLLLQEGSFFSRAVPGVWQPEQEGEEGLQLCKNELIHVKSVGEESKWEGMSLLTGQRGLVPVTALEPIPHPFYQWFLKNYAVSFGISQEISGTTSQPIVKGRCTATEDHRGAAPDELSFSKGDSIEVIGFLIPGLPWFVGKSVSSGSIGFVPTRYINPEACEPLGKGLVFLSEEEKSPLLRVPCNGGEQHFTTLLGDLAHTDITSVYRLDGFEPKAMFTKVPSEAVLHGCKDIQLLQSWEEINGLATTSTSELSSPGSESSPATLEDVLLEKMDDFDDPKFFIDLNAGHMEDADVFDPILTFLNQESYMPSFQSLYDLSFSFLNSTFYGFSDEDELVLYLETSRNWAKRTHSVWAHVRLCFLLGKLCIKKVKFSQARVYFEEAMSILDRGFGDLPLLAALHVNLASIYLKQNMKHKFSALMGKTVALLVCLPGHSFSSENELEVMMYVLKEAIAVGNAPLEARVCFLIVKLFLQLGKNDEVLPFTEHLQCLTNTLLRPDTSSVPLDVTPILSYLYDKKYLPNIALASARLFVPSGLKGALTPIWRAGFILQSISKLLGSHLERSSIPALACFYLKQALHFSCETKAVPTQRTLCAILSRMYLQHGVLDGAVCYAATAVTLSRRMGEEEAFESSLSLGWMYLLNNRPGPAADIMWQLLRSLHETDSVTQGGAVHNLLAIALKGEGQVQKAAENYLRALHKAQETGNKRNQAIALANLGQLSSSRGASQLSELYLLQSAQLYAELQGSEDLEMELVRVLLWLAQAMVNRLKVEDGKLCYELALVFALKWHNVSFTSLSLSAISTARCPPISRPALPTMSTGYHWHNSSRTERWKAMPGRPSASSTRLWAHLRL

Target ORF information:

RefSeq Version	XM_014947699.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax SH3 domain and tetratricopeptide repeats 2 (SH3TC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014947699.1

ORF Insert Sequence:

ATGGGCTGCT GCTTGGGGAC CCCCTGGCAC CAGGACCTGA CCCTGGGGTG CACAGGCGAA 
GAGTGTGACT CCTGCTGCCA CCGCTCACCC GTGCTCCTGC CCCGCTGGGA CCTCCTGCAG 
CGCACCGCAG CCGAGCCCCG GGGAAAATGG TGGTGGGAGA AGACCATGGC ATCAGGTAAG 
GCTGTGGTAT CAGAGCCAAG CCCCCCTGCT ACCGAGGCAG CAGAAGGCTC ACCGGACACC 
TCCTCGGCGC TGCTGGCTGT CGGGGAGGGC TTTCCACCAG AGATTTCGCT CTTCTTCTCC 
ATTGAGAGCC GCTCCTCACA GTGCCTCAAT CCCCAGCTCC AGGAAGCAGC CAGAAAGAAG 
CTGTGGGCCC TGGAGAGTAA TGACAGAGAC GTTTGTGCCC TGTTCAAGGA GCTGTCAGCC 
AGGCTGGTCT GTATGCAAGC ACAGGAGGAC AGGTTCCTCC TCACCTTCAA AACCCTGGAA 
GAAGTCTGGA AGTTTTCTAC CTATCTGACT TTAGGGTACG TGGGCAGCTG CCTGGAACAG 
CTTCTCTTTG ACCAGGAATA CTGGCTAAAC TGTGCTCTGA TGGAAGACAC AGAGGTCAGA 
GTTACTGTGG ATGAAGATCG CTTGGCCACC ATATATATGG GTCTGCTGCT CCAAGAAGGT 
AGCTTTTTTT CCAGAGCAGT GCCCGGTGTC TGGCAGCCAG AGCAGGAGGG AGAGGAAGGT 
CTGCAGCTCT GCAAGAACGA GCTGATCCAT GTGAAGAGCG TTGGAGAGGA GTCCAAGTGG 
GAAGGGATGT CCTTACTGAC AGGTCAACGA GGCCTTGTGC CTGTGACAGC TCTAGAGCCA 
ATACCTCATC CATTTTACCA GTGGTTCCTG AAGAATTATG CTGTGAGTTT TGGCATCTCC 
CAAGAGATCA GTGGGACAAC TTCTCAGCCA ATTGTCAAAG GCAGGTGCAC AGCCACAGAG 
GACCACAGAG GAGCAGCACC GGATGAACTG AGTTTCTCCA AAGGAGACAG CATAGAAGTC 
ATCGGCTTCC TCATTCCAGG ACTCCCATGG TTTGTGGGCA AATCCGTCAG CAGTGGGAGC 
ATTGGCTTTG TCCCAACCCG ATACATAAAT CCTGAGGCTT GTGAACCTCT GGGAAAAGGC 
TTGGTGTTTC TGAGTGAAGA AGAAAAATCC CCGCTCCTGC GAGTCCCCTG TAATGGTGGG 
GAACAGCACT TCACCACCCT CCTCGGTGAC CTGGCACACA CCGACATCAC CTCTGTGTAC 
CGCCTGGATG GTTTTGAACC TAAAGCCATG TTCACAAAAG TGCCATCAGA GGCTGTTCTC 
CATGGCTGTA AAGATATCCA GCTGCTCCAA TCTTGGGAGG AAATAAATGG CTTGGCTACA 
ACTAGCACCT CCGAGCTGTC TAGCCCAGGG AGTGAATCCT CCCCTGCTAC ATTGGAAGAT 
GTTCTCCTGG AGAAGATGGA TGATTTTGAT GATCCTAAGT TCTTTATTGA CCTGAACGCT 
GGACACATGG AAGATGCTGA TGTCTTTGAC CCCATATTGA CCTTCCTTAA CCAAGAGAGT 
TATATGCCCA GTTTCCAAAG CCTCTATGAT CTCAGTTTTT CCTTTCTCAA CTCCACTTTT 
TATGGTTTCT CTGATGAGGA TGAACTGGTC TTGTACCTTG AGACATCCAG GAACTGGGCC 
AAGAGGACTC ATTCCGTTTG GGCTCATGTC AGGCTCTGTT TCCTCTTGGG TAAGCTCTGC 
ATCAAAAAGG TCAAGTTCTC CCAGGCTCGG GTCTACTTTG AGGAAGCCAT GAGCATCCTG 
GATAGGGGTT TTGGGGATCT GCCCCTCTTG GCTGCATTGC ATGTGAACCT TGCCTCAATC 
TACTTGAAAC AAAATATGAA GCACAAGTTC TCCGCCTTGA TGGGGAAAAC GGTGGCCTTG 
CTTGTCTGCT TGCCTGGCCA CTCTTTCAGC TCTGAGAATG AGCTGGAAGT CATGATGTAT 
GTCCTGAAGG AAGCTATTGC TGTGGGTAAT GCTCCCTTGG AGGCACGGGT CTGCTTCCTT 
ATTGTCAAGC TCTTCCTACA ACTGGGAAAA AACGATGAAG TGCTGCCCTT TACCGAGCAT 
CTTCAATGCC TCACCAACAC TTTACTCAGA CCAGACACCA GTTCTGTGCC ACTGGATGTA 
ACCCCCATCT TGAGCTACCT GTACGACAAG AAGTACTTGC CAAATATCGC ACTGGCATCT 
GCCAGGTTGT TTGTTCCCAG TGGCCTCAAG GGAGCACTGA CACCCATTTG GAGAGCTGGC 
TTCATCCTCC AAAGTATTTC CAAACTCCTG GGAAGCCACC TGGAGAGGAG TAGCATCCCA 
GCGCTGGCTT GTTTCTACCT CAAGCAAGCC CTGCATTTCT CCTGCGAGAC CAAAGCAGTG 
CCCACCCAGA GGACACTGTG TGCCATCTTG TCCAGGATGT ACCTCCAGCA CGGTGTCTTG 
GATGGGGCGG TTTGTTACGC AGCCACAGCT GTAACCCTCA GCAGACGGAT GGGCGAGGAG 
GAGGCTTTCG AGTCTTCCCT CTCCTTGGGG TGGATGTATC TCCTGAACAA CCGGCCGGGC 
CCAGCTGCAG ACATCATGTG GCAGCTCTTG CGCTCTCTAC ACGAGACGGA CAGCGTGACT 
CAGGGTGGAG CAGTGCACAA TCTCCTGGCC ATCGCCCTCA AAGGAGAAGG ACAGGTGCAA 
AAGGCAGCAG AGAACTACCT TCGGGCCCTG CACAAAGCCC AGGAGACTGG GAACAAGAGG 
AACCAGGCTA TTGCCCTGGC TAACCTGGGG CAGCTGAGCT CCTCGCGCGG GGCGAGCCAG 
CTGTCTGAGC TCTACCTGCT CCAGTCGGCT CAGCTCTACG CTGAGCTCCA GGGCAGCGAA 
GACCTGGAGA TGGAGTTGGT GCGGGTGTTG CTGTGGCTGG CACAGGCCAT GGTGAACAGG 
CTGAAGGTGG AAGATGGCAA ACTCTGTTAT GAACTGGCGT TGGTTTTTGC CCTGAAGTGG 
CATAACGTCA GCTTCACGTC ACTGAGTCTC TCTGCCATTT CTACAGCAAG GTGTCCCCCA 
ATCTCCAGGC CTGCATTACC TACCATGAGC ACTGGGTATC ATTGGCACAA CAGCTCCAGG 
ACAGAGAGAT GGAAGGCAAT GCCCGGCAGA CCCTCAGCCA GCTCTACCAG GCTTTGGGCA 
CATCTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SH3TC2 cDNA ORF clone, Calidris pugnax(ruff)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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