TRIM33 cDNA ORF clone, Calidris pugnax(ruff)

  • Gene

  • Clones

  • gRNAs

The following TRIM33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIM33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa69842 XM_014948545.1
Latest version!
Calidris pugnax tripartite motif containing 33 (TRIM33), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCa69843 XM_014948546.1
Latest version!
Calidris pugnax tripartite motif containing 33 (TRIM33), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCa69844 XM_014948547.1
Latest version!
Calidris pugnax tripartite motif containing 33 (TRIM33), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCa69845 XM_014948548.1
Latest version!
Calidris pugnax tripartite motif containing 33 (TRIM33), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa69842
    Clone ID Related Accession (Same CDS sequence) XM_014948545.1
    Accession Version XM_014948545.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product E3 ubiquitin-protein ligase TRIM33 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090898.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGGAAA ACAAAGGAGG CGGCGGCGGC GGGGAGGGGG CTGCCGAGGC CGGGCCGGGC 
    GGCGGTGGCG GCCTAGAACC TGCAGCCGCC TCTCCGTCCG GCGCCGCGCC CCCCGCGCTC
    GCCTCCGCGC CTCCCGAAGA GCGGGAGAGC CCAGGCGCGG CGGCGGCGGC GGTGGAACGA
    GGTCTCGGGG AGGCCGAGGC CGAGGCGGCG GCGGGGCCCG GTGTCGCTCC CGGGCCGGGC
    CCCAGCCCGG TGCCTCCATT GACACCGGCG GCTCCAGCGC CTTTCTCGCT GCTGGACACC
    TGCGCCGTGT GCGCGCAGAG CCTGCAGAGC CGGCGGGAGG CCGAGCCCAA GCTGCTGCCC
    TGCCTGCACT CCTTCTGCCG CCGCTGCCTG CCCGAGCCCG AGCGGCAGCT CAGCGTCCCC
    ATCCCCGGCG GCGCCAACGG CGACGTCCAG CAAGTTGGCG TGATCAGATG TCCGATATGC
    CGCCAAGAGT GCAGACAGAT AGATCTGGTG GATAACTACT TTGTAAAAGA CACGTCAGAA
    GCACCCAGCA GCTCCGATGA GAAGTCAGAG CAGGTGTGCA CAAGCTGTGA AGATAACGCC
    AGCGCTGTCG GATTTTGTGT AGAGTGTGGG GAATGGTTGT GCAAGACTTG CATAGAAGCT
    CATCAGCGAG TAAAATTTAC TAAAGATCAT ATGATCAGAA AAAAAGAGGA TGTATCTTCA
    GAGGCCGTGG GAGCATCTGG TCAACGTCCT GTTTTCTGTC CTGTCCACAA ACAAGAGCAG
    TTAAAACTTT TCTGTGAAAC CTGTGACAGG CTGACATGCA GAGACTGTCA GTTACTGGAG
    CACAAAGAAC ACAGGTATCA GTTTCTGGAA GAAGCTTTTC AGAACCAGAA GGGAGCAATT
    GAGAACCTGT TGGCCAAGCT TCTTGAGAAG AAGAACTATG TAAATTTTGC AGCTACCCAA
    GTTCAGAATA GGATAAAAGA AGTAAATGAA ACAAACAAAC GAGTAGAACA GGAAATCAAA
    GTGGCTATAT TCACCCTCAT CAATGAAATC AATAAAAAGG GAAAATCTCT CTTACAGCAC
    CTTGAGAATG TCACAAAGGA GAGACAGATG AAGTTAATAC AGCAACAAAA TGACATCACT
    GGTCTTTCAC GACAAGTGAA GCATGTGATG AACTTCACTA ATTGGGCTAT TGCAAGCGGC
    AGCAGTACTG CTTTGCTCTA CAGTAAGCGG CTGATAACAT TCCAGTTACG TCATATTTTA
    AAGGCACGTT GTGATCCTGT CCCAGCTGCC AATGGAGCGA TTCGCTTCCA TTGTGACCCT
    ACATTCTGGG CAAAGAACGT CGTCAATTTA GGTAACCTTG TCATTGAAAA TAAACCAACT
    CCTACTTACA CTCCTAATGT AGTGGTTGGA CAAGCTCCTC CAGGAACAAA CCACATCAAC
    AAAGCTCCGG GACAAATTAA TCTGGCGCAG CTCCGGCTTC AGCACATGCA GCAGCAAGTT
    TATGCCCAGA AGCACCAGCA GCTGCAGCAG ATGAGGATGG GGCAGCCAAC GGGGTCCGTT
    CCCAGGCAGA GCAGCCAGCA GGTCTTGCAG CAGCAGCCTC CCAGGTTGAT CAGCATGCAG
    ACCATGCAGA GGGGTAACAT GAACTGTGGG GCTTTCCAAG CACATCAGAT GAGAATGGCT
    CAGAATGCTG CTCGTATACC AGGAATACCA CGCCACAATG GACAACAATA CTCCATGATG
    CAGCCTCACC TTCAAAGACA ACACTCTAAC CCTGGCCACG CGGGGCCTTT TCCAGTTGTC
    TCTGTGCACA ACACCACTAT TAATCCAACT AGTCCCACCA CAGCAACAAT GGCGAGTGCA
    AACCGTGGTC CAACAAGTCC GTCCGTTACA GCCATCGAGC TCATTCCTTC TGTAACAAAC
    CCAGAGAATT TACCTTCCCT GCCGGACATC CCACCGATCC AGCTTGAAGA TGCTGGTTCA
    AGTAATTTGG ATAACCTTCT AAGCAGATAC ATTACAGGCA GCCACCTACC CCCACAACCT
    ACGAGTACCA TGAATCCCTC CCCAGGACCT TCAGCTCTGT CTCCAGGGTC ATCAGGTTTA
    TCCAACTCCC ACACTCCTGT AAGGCCGCCC AGTACCTCCA GCACAGGCAG CAGAGGAAGC
    TGTGGCTCCT CGGGCAGAAC TGCTGAGAAA ACTAGCGTTA ACTTCAAGTC TGACCAAGTG
    AAAGTCAAGC AAGAGCCAGG TACAGAGGAA GAGATATGCA GTTTCTCAGG AACAGTAAAG
    CAGGAAAAAA CAGAGGATGG CAGAAGGAGT GCTTGCATGC TTAGCAGTCC TGAGAGCAGC
    TTGACACCAC CACTATCCAC TAACTTGCAT CTGGAGAGTG AATTAGAAGC ATTAGGAAGT
    CTTGAAAATC ATGTAAAAAC TGAGCCAACG GATTTAAGTG AAAGTTGCAA ACAGTCTGGA
    CACAGCCTGG TGAATGGGAA GTCCCCAGTG CGGAGTCTAA TGCACCGGTC TGCTAGGATT
    GGAGAAGGCA ATAACAAAGA TGATGATCCA AACGAGGACT GGTGCGCCGT GTGCCAAAAC
    GGAGGGGATC TCTTGTGTTG TGAAAAGTGT CCAAAAGTGT TTCACCTGAC TTGCCACGTC
    CCGACACTCC TCAGCTTTCC AAGTGGAGAG TGGATATGTA CATTCTGCAG AGATCTGAGC
    AAACCAGAAG TAGAATATGA TTGTGACAAC TCACAGCACA GCAAGAAAGG GAAAACAGCA
    CAAGGCCTGA GTCCTGTGGA CCAAAGGAAA TGTGAACGTC TCCTGCTTTA CCTGTATTGT
    CATGAGCTGA GCATTGAATT TCAAGAGCCA GTCCCAGCCT CGATACCAAA CTACTATAAA
    ATTATAAAGA AACCAATGGA CTTATCTACG GTGAAAAAGA AACTGCAAAA GAAGCATTCC
    CAACACTACC AGACTCCTGA GGATTTTGTG GCAGATGTCC GGTTGATCTT CAAGAACTGT
    GAAAGGTTTA ATGAAATGAT GAAAGTTGTT CAAGTTTATG CAGAAACACA AGAGATTAAT
    TTGAAGGCTG ATTCAGAAGT AGCGCAGGCA GGGAAGGCAG TTGCATTATA CTTTGAAGAT
    AAACTTACAG AGATCTACCC AGACAGGACC TTCCAGCCTT TGCCTGAATT TGAGCAGGAA
    GAGGATGATG GTGAAATAAC TGAGGACTCC GATGAAGATT TTATACAACC ACGTAGAAAA
    CGCCTAAAAT CAGATGAGAG ACCAGTGCAT ATAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014804031.1
    CDS28..3306
    Translation

    Target ORF information:

    RefSeq Version XM_014948545.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax tripartite motif containing 33 (TRIM33), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014948545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGGAAA ACAAAGGAGG CGGCGGCGGC GGGGAGGGGG CTGCCGAGGC CGGGCCGGGC 
    GGCGGTGGCG GCCTAGAACC TGCAGCCGCC TCTCCGTCCG GCGCCGCGCC CCCCGCGCTC
    GCCTCCGCGC CTCCCGAAGA GCGGGAGAGC CCAGGCGCGG CGGCGGCGGC GGTGGAACGA
    GGTCTCGGGG AGGCCGAGGC CGAGGCGGCG GCGGGGCCCG GTGTCGCTCC CGGGCCGGGC
    CCCAGCCCGG TGCCTCCATT GACACCGGCG GCTCCAGCGC CTTTCTCGCT GCTGGACACC
    TGCGCCGTGT GCGCGCAGAG CCTGCAGAGC CGGCGGGAGG CCGAGCCCAA GCTGCTGCCC
    TGCCTGCACT CCTTCTGCCG CCGCTGCCTG CCCGAGCCCG AGCGGCAGCT CAGCGTCCCC
    ATCCCCGGCG GCGCCAACGG CGACGTCCAG CAAGTTGGCG TGATCAGATG TCCGATATGC
    CGCCAAGAGT GCAGACAGAT AGATCTGGTG GATAACTACT TTGTAAAAGA CACGTCAGAA
    GCACCCAGCA GCTCCGATGA GAAGTCAGAG CAGGTGTGCA CAAGCTGTGA AGATAACGCC
    AGCGCTGTCG GATTTTGTGT AGAGTGTGGG GAATGGTTGT GCAAGACTTG CATAGAAGCT
    CATCAGCGAG TAAAATTTAC TAAAGATCAT ATGATCAGAA AAAAAGAGGA TGTATCTTCA
    GAGGCCGTGG GAGCATCTGG TCAACGTCCT GTTTTCTGTC CTGTCCACAA ACAAGAGCAG
    TTAAAACTTT TCTGTGAAAC CTGTGACAGG CTGACATGCA GAGACTGTCA GTTACTGGAG
    CACAAAGAAC ACAGGTATCA GTTTCTGGAA GAAGCTTTTC AGAACCAGAA GGGAGCAATT
    GAGAACCTGT TGGCCAAGCT TCTTGAGAAG AAGAACTATG TAAATTTTGC AGCTACCCAA
    GTTCAGAATA GGATAAAAGA AGTAAATGAA ACAAACAAAC GAGTAGAACA GGAAATCAAA
    GTGGCTATAT TCACCCTCAT CAATGAAATC AATAAAAAGG GAAAATCTCT CTTACAGCAC
    CTTGAGAATG TCACAAAGGA GAGACAGATG AAGTTAATAC AGCAACAAAA TGACATCACT
    GGTCTTTCAC GACAAGTGAA GCATGTGATG AACTTCACTA ATTGGGCTAT TGCAAGCGGC
    AGCAGTACTG CTTTGCTCTA CAGTAAGCGG CTGATAACAT TCCAGTTACG TCATATTTTA
    AAGGCACGTT GTGATCCTGT CCCAGCTGCC AATGGAGCGA TTCGCTTCCA TTGTGACCCT
    ACATTCTGGG CAAAGAACGT CGTCAATTTA GGTAACCTTG TCATTGAAAA TAAACCAACT
    CCTACTTACA CTCCTAATGT AGTGGTTGGA CAAGCTCCTC CAGGAACAAA CCACATCAAC
    AAAGCTCCGG GACAAATTAA TCTGGCGCAG CTCCGGCTTC AGCACATGCA GCAGCAAGTT
    TATGCCCAGA AGCACCAGCA GCTGCAGCAG ATGAGGATGG GGCAGCCAAC GGGGTCCGTT
    CCCAGGCAGA GCAGCCAGCA GGTCTTGCAG CAGCAGCCTC CCAGGTTGAT CAGCATGCAG
    ACCATGCAGA GGGGTAACAT GAACTGTGGG GCTTTCCAAG CACATCAGAT GAGAATGGCT
    CAGAATGCTG CTCGTATACC AGGAATACCA CGCCACAATG GACAACAATA CTCCATGATG
    CAGCCTCACC TTCAAAGACA ACACTCTAAC CCTGGCCACG CGGGGCCTTT TCCAGTTGTC
    TCTGTGCACA ACACCACTAT TAATCCAACT AGTCCCACCA CAGCAACAAT GGCGAGTGCA
    AACCGTGGTC CAACAAGTCC GTCCGTTACA GCCATCGAGC TCATTCCTTC TGTAACAAAC
    CCAGAGAATT TACCTTCCCT GCCGGACATC CCACCGATCC AGCTTGAAGA TGCTGGTTCA
    AGTAATTTGG ATAACCTTCT AAGCAGATAC ATTACAGGCA GCCACCTACC CCCACAACCT
    ACGAGTACCA TGAATCCCTC CCCAGGACCT TCAGCTCTGT CTCCAGGGTC ATCAGGTTTA
    TCCAACTCCC ACACTCCTGT AAGGCCGCCC AGTACCTCCA GCACAGGCAG CAGAGGAAGC
    TGTGGCTCCT CGGGCAGAAC TGCTGAGAAA ACTAGCGTTA ACTTCAAGTC TGACCAAGTG
    AAAGTCAAGC AAGAGCCAGG TACAGAGGAA GAGATATGCA GTTTCTCAGG AACAGTAAAG
    CAGGAAAAAA CAGAGGATGG CAGAAGGAGT GCTTGCATGC TTAGCAGTCC TGAGAGCAGC
    TTGACACCAC CACTATCCAC TAACTTGCAT CTGGAGAGTG AATTAGAAGC ATTAGGAAGT
    CTTGAAAATC ATGTAAAAAC TGAGCCAACG GATTTAAGTG AAAGTTGCAA ACAGTCTGGA
    CACAGCCTGG TGAATGGGAA GTCCCCAGTG CGGAGTCTAA TGCACCGGTC TGCTAGGATT
    GGAGAAGGCA ATAACAAAGA TGATGATCCA AACGAGGACT GGTGCGCCGT GTGCCAAAAC
    GGAGGGGATC TCTTGTGTTG TGAAAAGTGT CCAAAAGTGT TTCACCTGAC TTGCCACGTC
    CCGACACTCC TCAGCTTTCC AAGTGGAGAG TGGATATGTA CATTCTGCAG AGATCTGAGC
    AAACCAGAAG TAGAATATGA TTGTGACAAC TCACAGCACA GCAAGAAAGG GAAAACAGCA
    CAAGGCCTGA GTCCTGTGGA CCAAAGGAAA TGTGAACGTC TCCTGCTTTA CCTGTATTGT
    CATGAGCTGA GCATTGAATT TCAAGAGCCA GTCCCAGCCT CGATACCAAA CTACTATAAA
    ATTATAAAGA AACCAATGGA CTTATCTACG GTGAAAAAGA AACTGCAAAA GAAGCATTCC
    CAACACTACC AGACTCCTGA GGATTTTGTG GCAGATGTCC GGTTGATCTT CAAGAACTGT
    GAAAGGTTTA ATGAAATGAT GAAAGTTGTT CAAGTTTATG CAGAAACACA AGAGATTAAT
    TTGAAGGCTG ATTCAGAAGT AGCGCAGGCA GGGAAGGCAG TTGCATTATA CTTTGAAGAT
    AAACTTACAG AGATCTACCC AGACAGGACC TTCCAGCCTT TGCCTGAATT TGAGCAGGAA
    GAGGATGATG GTGAAATAAC TGAGGACTCC GATGAAGATT TTATACAACC ACGTAGAAAA
    CGCCTAAAAT CAGATGAGAG ACCAGTGCAT ATAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa69843
    Clone ID Related Accession (Same CDS sequence) XM_014948546.1
    Accession Version XM_014948546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product E3 ubiquitin-protein ligase TRIM33 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090898.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAAA ACAAAGGAGG CGGCGGCGGC GGGGAGGGGG CTGCCGAGGC CGGGCCGGGC 
    GGCGGTGGCG GCCTAGAACC TGCAGCCGCC TCTCCGTCCG GCGCCGCGCC CCCCGCGCTC
    GCCTCCGCGC CTCCCGAAGA GCGGGAGAGC CCAGGCGCGG CGGCGGCGGC GGTGGAACGA
    GGTCTCGGGG AGGCCGAGGC CGAGGCGGCG GCGGGGCCCG GTGTCGCTCC CGGGCCGGGC
    CCCAGCCCGG TGCCTCCATT GACACCGGCG GCTCCAGCGC CTTTCTCGCT GCTGGACACC
    TGCGCCGTGT GCGCGCAGAG CCTGCAGAGC CGGCGGGAGG CCGAGCCCAA GCTGCTGCCC
    TGCCTGCACT CCTTCTGCCG CCGCTGCCTG CCCGAGCCCG AGCGGCAGCT CAGCGTCCCC
    ATCCCCGGCG GCGCCAACGG CGACGTCCAG CAAGTTGGCG TGATCAGATG TCCGATATGC
    CGCCAAGAGT GCAGACAGAT AGATCTGGTG GATAACTACT TTGTAAAAGA CACGTCAGAA
    GCACCCAGCA GCTCCGATGA GAAGTCAGAG CAGGTGTGCA CAAGCTGTGA AGATAACGCC
    AGCGCTGTCG GATTTTGTGT AGAGTGTGGG GAATGGTTGT GCAAGACTTG CATAGAAGCT
    CATCAGCGAG TAAAATTTAC TAAAGATCAT ATGATCAGAA AAAAAGAGGA TGTATCTTCA
    GAGGCCGTGG GAGCATCTGG TCAACGTCCT GTTTTCTGTC CTGTCCACAA ACAAGAGCAG
    TTAAAACTTT TCTGTGAAAC CTGTGACAGG CTGACATGCA GAGACTGTCA GTTACTGGAG
    CACAAAGAAC ACAGGTATCA GTTTCTGGAA GAAGCTTTTC AGAACCAGAA GGGAGCAATT
    GAGAACCTGT TGGCCAAGCT TCTTGAGAAG AAGAACTATG TAAATTTTGC AGCTACCCAA
    GTTCAGAATA GGATAAAAGA AGTAAATGAA ACAAACAAAC GAGTAGAACA GGAAATCAAA
    GTGGCTATAT TCACCCTCAT CAATGAAATC AATAAAAAGG GAAAATCTCT CTTACAGCAC
    CTTGAGAATG TCACAAAGGA GAGACAGATG AAGTTAATAC AGCAACAAAA TGACATCACT
    GGTCTTTCAC GACAAGTGAA GCATGTGATG AACTTCACTA ATTGGGCTAT TGCAAGCGGC
    AGCAGTACTG CTTTGCTCTA CAGTAAGCGG CTGATAACAT TCCAGTTACG TCATATTTTA
    AAGGCACGTT GTGATCCTGT CCCAGCTGCC AATGGAGCGA TTCGCTTCCA TTGTGACCCT
    ACATTCTGGG CAAAGAACGT CGTCAATTTA GGTAACCTTG TCATTGAAAA TAAACCAACT
    CCTACTTACA CTCCTAATGT AGTGGTTGGA CAAGCTCCTC CAGGAACAAA CCACATCAAC
    AAAGCTCCGG GACAAATTAA TCTGGCGCAG CTCCGGCTTC AGCACATGCA GCAGCAAGTT
    TATGCCCAGA AGCACCAGCA GCTGCAGCAG ATGAGGATGG GGCAGCCAAC GGGGTCCGTT
    CCCAGGCAGA GCAGCCAGCA GGTCTTGCAG CAGCAGCCTC CCAGGTTGAT CAGCATGCAG
    ACCATGCAGA GGGGTAACAT GAACTGTGGG GCTTTCCAAG CACATCAGAT GAGAATGGCT
    CAGAATGCTG CTCGTATACC AGGAATACCA CGCCACAATG GACAACAATA CTCCATGATG
    CAGCCTCACC TTCAAAGACA ACACTCTAAC CCTGGCCACG CGGGGCCTTT TCCAGTTGTC
    TCTGTGCACA ACACCACTAT TAATCCAACT AGTCCCACCA CAGCAACAAT GGCGAGTGCA
    AACCGTGGTC CAACAAGTCC GTCCGTTACA GCCATCGAGC TCATTCCTTC TGTAACAAAC
    CCAGAGAATT TACCTTCCCT GCCGGACATC CCACCGATCC AGCTTGAAGA TGCTGGTTCA
    AGTAATTTGG ATAACCTTCT AAGCAGATAC ATTACAGGCA GCCACCTACC CCCACAACCT
    ACGAGTACCA TGAATCCCTC CCCAGGACCT TCAGCTCTGT CTCCAGGGTC ATCAGGTTTA
    TCCAACTCCC ACACTCCTGT AAGGCCGCCC AGTACCTCCA GCACAGGCAG CAGAGGAAGC
    TGTGGCTCCT CGGGCAGAAC TGCTGAGAAA ACTAGCGTTA ACTTCAAGTC TGACCAAGTG
    AAAGTCAAGC AAGAGCCAGG TACAGAGGAA GAGATATGCA GTTTCTCAGG AACAGTAAAG
    CAGGAAAAAA CAGAGGATGG CAGAAGGAGT GCTTGCATGC TTAGCAGTCC TGAGAGCAGC
    TTGACACCAC CACTATCCAC TAACTTGCAT CTGGAGAGTG AATTAGAAGC ATTAGGAAGT
    CTTGAAAATC ATGTAAAAAC TGAGCCAACG GATTTAAGTG AAAGTTGCAA ACAGTCTGGA
    CACAGCCTGG TGAATGGGAA GTCCCCAGTG CGGAGTCTAA TGCACCGGTC TGCTAGGATT
    GGAGAAGGCA ATAACAAAGA TGATGATCCA AACGAGGACT GGTGCGCCGT GTGCCAAAAC
    GGAGGGGATC TCTTGTGTTG TGAAAAGTGT CCAAAAGTGT TTCACCTGAC TTGCCACGTC
    CCGACACTCC TCAGCTTTCC AAGTGGAGAG TGGATATGTA CATTCTGCAG AGATCTGAGC
    AAACCAGAAG TAGAATATGA TTGTGACAAC TCACAGCACA GCAAGAAAGG GAAAACAGCA
    CAAGGCCTGA GTCCTGTGGA CCAAAGGAAA TGTGAACGTC TCCTGCTTTA CCTGTATTGT
    CATGAGCTGA GCATTGAATT TCAAGAGCCA GTCCCAGCCT CGATACCAAA CTACTATAAA
    ATTATAAAGA AACCAATGGA CTTATCTACG GTGAAAAAGA AACTGCAAAA GAAGCATTCC
    CAACACTACC AGACTCCTGA GGATTTTGTG GCAGATGTCC GGTTGATCTT CAAGAACTGT
    GAAAGGTTTA ATGAAGTGGA GATTAATGAG CACTGTGGAA GAAGTTGCAA TTCCTGTAGA
    GGAAAACGTG CTGCAGCTCT TAGGGACAAG AGTAAAACAA GGGAGCAAGG ACTGATCGAT
    GTGTTGTTTT TTGGTTTGGT TTTGTTGTTT TTTCAAAAAA AAAAAAAAAA AGGTGTTTTA
    GGCTTGTCTC TCTGTTTGAA ATGCTGGCTC ATGGCAAAAG ATGAATCGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014804032.1
    CDS28..3261
    Translation

    Target ORF information:

    RefSeq Version XM_014948546.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax tripartite motif containing 33 (TRIM33), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014948546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAAA ACAAAGGAGG CGGCGGCGGC GGGGAGGGGG CTGCCGAGGC CGGGCCGGGC 
    GGCGGTGGCG GCCTAGAACC TGCAGCCGCC TCTCCGTCCG GCGCCGCGCC CCCCGCGCTC
    GCCTCCGCGC CTCCCGAAGA GCGGGAGAGC CCAGGCGCGG CGGCGGCGGC GGTGGAACGA
    GGTCTCGGGG AGGCCGAGGC CGAGGCGGCG GCGGGGCCCG GTGTCGCTCC CGGGCCGGGC
    CCCAGCCCGG TGCCTCCATT GACACCGGCG GCTCCAGCGC CTTTCTCGCT GCTGGACACC
    TGCGCCGTGT GCGCGCAGAG CCTGCAGAGC CGGCGGGAGG CCGAGCCCAA GCTGCTGCCC
    TGCCTGCACT CCTTCTGCCG CCGCTGCCTG CCCGAGCCCG AGCGGCAGCT CAGCGTCCCC
    ATCCCCGGCG GCGCCAACGG CGACGTCCAG CAAGTTGGCG TGATCAGATG TCCGATATGC
    CGCCAAGAGT GCAGACAGAT AGATCTGGTG GATAACTACT TTGTAAAAGA CACGTCAGAA
    GCACCCAGCA GCTCCGATGA GAAGTCAGAG CAGGTGTGCA CAAGCTGTGA AGATAACGCC
    AGCGCTGTCG GATTTTGTGT AGAGTGTGGG GAATGGTTGT GCAAGACTTG CATAGAAGCT
    CATCAGCGAG TAAAATTTAC TAAAGATCAT ATGATCAGAA AAAAAGAGGA TGTATCTTCA
    GAGGCCGTGG GAGCATCTGG TCAACGTCCT GTTTTCTGTC CTGTCCACAA ACAAGAGCAG
    TTAAAACTTT TCTGTGAAAC CTGTGACAGG CTGACATGCA GAGACTGTCA GTTACTGGAG
    CACAAAGAAC ACAGGTATCA GTTTCTGGAA GAAGCTTTTC AGAACCAGAA GGGAGCAATT
    GAGAACCTGT TGGCCAAGCT TCTTGAGAAG AAGAACTATG TAAATTTTGC AGCTACCCAA
    GTTCAGAATA GGATAAAAGA AGTAAATGAA ACAAACAAAC GAGTAGAACA GGAAATCAAA
    GTGGCTATAT TCACCCTCAT CAATGAAATC AATAAAAAGG GAAAATCTCT CTTACAGCAC
    CTTGAGAATG TCACAAAGGA GAGACAGATG AAGTTAATAC AGCAACAAAA TGACATCACT
    GGTCTTTCAC GACAAGTGAA GCATGTGATG AACTTCACTA ATTGGGCTAT TGCAAGCGGC
    AGCAGTACTG CTTTGCTCTA CAGTAAGCGG CTGATAACAT TCCAGTTACG TCATATTTTA
    AAGGCACGTT GTGATCCTGT CCCAGCTGCC AATGGAGCGA TTCGCTTCCA TTGTGACCCT
    ACATTCTGGG CAAAGAACGT CGTCAATTTA GGTAACCTTG TCATTGAAAA TAAACCAACT
    CCTACTTACA CTCCTAATGT AGTGGTTGGA CAAGCTCCTC CAGGAACAAA CCACATCAAC
    AAAGCTCCGG GACAAATTAA TCTGGCGCAG CTCCGGCTTC AGCACATGCA GCAGCAAGTT
    TATGCCCAGA AGCACCAGCA GCTGCAGCAG ATGAGGATGG GGCAGCCAAC GGGGTCCGTT
    CCCAGGCAGA GCAGCCAGCA GGTCTTGCAG CAGCAGCCTC CCAGGTTGAT CAGCATGCAG
    ACCATGCAGA GGGGTAACAT GAACTGTGGG GCTTTCCAAG CACATCAGAT GAGAATGGCT
    CAGAATGCTG CTCGTATACC AGGAATACCA CGCCACAATG GACAACAATA CTCCATGATG
    CAGCCTCACC TTCAAAGACA ACACTCTAAC CCTGGCCACG CGGGGCCTTT TCCAGTTGTC
    TCTGTGCACA ACACCACTAT TAATCCAACT AGTCCCACCA CAGCAACAAT GGCGAGTGCA
    AACCGTGGTC CAACAAGTCC GTCCGTTACA GCCATCGAGC TCATTCCTTC TGTAACAAAC
    CCAGAGAATT TACCTTCCCT GCCGGACATC CCACCGATCC AGCTTGAAGA TGCTGGTTCA
    AGTAATTTGG ATAACCTTCT AAGCAGATAC ATTACAGGCA GCCACCTACC CCCACAACCT
    ACGAGTACCA TGAATCCCTC CCCAGGACCT TCAGCTCTGT CTCCAGGGTC ATCAGGTTTA
    TCCAACTCCC ACACTCCTGT AAGGCCGCCC AGTACCTCCA GCACAGGCAG CAGAGGAAGC
    TGTGGCTCCT CGGGCAGAAC TGCTGAGAAA ACTAGCGTTA ACTTCAAGTC TGACCAAGTG
    AAAGTCAAGC AAGAGCCAGG TACAGAGGAA GAGATATGCA GTTTCTCAGG AACAGTAAAG
    CAGGAAAAAA CAGAGGATGG CAGAAGGAGT GCTTGCATGC TTAGCAGTCC TGAGAGCAGC
    TTGACACCAC CACTATCCAC TAACTTGCAT CTGGAGAGTG AATTAGAAGC ATTAGGAAGT
    CTTGAAAATC ATGTAAAAAC TGAGCCAACG GATTTAAGTG AAAGTTGCAA ACAGTCTGGA
    CACAGCCTGG TGAATGGGAA GTCCCCAGTG CGGAGTCTAA TGCACCGGTC TGCTAGGATT
    GGAGAAGGCA ATAACAAAGA TGATGATCCA AACGAGGACT GGTGCGCCGT GTGCCAAAAC
    GGAGGGGATC TCTTGTGTTG TGAAAAGTGT CCAAAAGTGT TTCACCTGAC TTGCCACGTC
    CCGACACTCC TCAGCTTTCC AAGTGGAGAG TGGATATGTA CATTCTGCAG AGATCTGAGC
    AAACCAGAAG TAGAATATGA TTGTGACAAC TCACAGCACA GCAAGAAAGG GAAAACAGCA
    CAAGGCCTGA GTCCTGTGGA CCAAAGGAAA TGTGAACGTC TCCTGCTTTA CCTGTATTGT
    CATGAGCTGA GCATTGAATT TCAAGAGCCA GTCCCAGCCT CGATACCAAA CTACTATAAA
    ATTATAAAGA AACCAATGGA CTTATCTACG GTGAAAAAGA AACTGCAAAA GAAGCATTCC
    CAACACTACC AGACTCCTGA GGATTTTGTG GCAGATGTCC GGTTGATCTT CAAGAACTGT
    GAAAGGTTTA ATGAAGTGGA GATTAATGAG CACTGTGGAA GAAGTTGCAA TTCCTGTAGA
    GGAAAACGTG CTGCAGCTCT TAGGGACAAG AGTAAAACAA GGGAGCAAGG ACTGATCGAT
    GTGTTGTTTT TTGGTTTGGT TTTGTTGTTT TTTCAAAAAA AAAAAAAAAA AGGTGTTTTA
    GGCTTGTCTC TCTGTTTGAA ATGCTGGCTC ATGGCAAAAG ATGAATCGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa69844
    Clone ID Related Accession (Same CDS sequence) XM_014948547.1
    Accession Version XM_014948547.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product E3 ubiquitin-protein ligase TRIM33 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090898.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAAA ACAAAGGAGG CGGCGGCGGC GGGGAGGGGG CTGCCGAGGC CGGGCCGGGC 
    GGCGGTGGCG GCCTAGAACC TGCAGCCGCC TCTCCGTCCG GCGCCGCGCC CCCCGCGCTC
    GCCTCCGCGC CTCCCGAAGA GCGGGAGAGC CCAGGCGCGG CGGCGGCGGC GGTGGAACGA
    GGTCTCGGGG AGGCCGAGGC CGAGGCGGCG GCGGGGCCCG GTGTCGCTCC CGGGCCGGGC
    CCCAGCCCGG TGCCTCCATT GACACCGGCG GCTCCAGCGC CTTTCTCGCT GCTGGACACC
    TGCGCCGTGT GCGCGCAGAG CCTGCAGAGC CGGCGGGAGG CCGAGCCCAA GCTGCTGCCC
    TGCCTGCACT CCTTCTGCCG CCGCTGCCTG CCCGAGCCCG AGCGGCAGCT CAGCGTCCCC
    ATCCCCGGCG GCGCCAACGG CGACGTCCAG CAAGTTGGCG TGATCAGATG TCCGATATGC
    CGCCAAGAGT GCAGACAGAT AGATCTGGTG GATAACTACT TTGTAAAAGA CACGTCAGAA
    GCACCCAGCA GCTCCGATGA GAAGTCAGAG CAGGTGTGCA CAAGCTGTGA AGATAACGCC
    AGCGCTGTCG GATTTTGTGT AGAGTGTGGG GAATGGTTGT GCAAGACTTG CATAGAAGCT
    CATCAGCGAG TAAAATTTAC TAAAGATCAT ATGATCAGAA AAAAAGAGGA TGTATCTTCA
    GAGGCCGTGG GAGCATCTGG TCAACGTCCT GTTTTCTGTC CTGTCCACAA ACAAGAGCAG
    TTAAAACTTT TCTGTGAAAC CTGTGACAGG CTGACATGCA GAGACTGTCA GTTACTGGAG
    CACAAAGAAC ACAGGTATCA GTTTCTGGAA GAAGCTTTTC AGAACCAGAA GGGAGCAATT
    GAGAACCTGT TGGCCAAGCT TCTTGAGAAG AAGAACTATG TAAATTTTGC AGCTACCCAA
    GTTCAGAATA GGATAAAAGA AGTAAATGAA ACAAACAAAC GAGTAGAACA GGAAATCAAA
    GTGGCTATAT TCACCCTCAT CAATGAAATC AATAAAAAGG GAAAATCTCT CTTACAGCAC
    CTTGAGAATG TCACAAAGGA GAGACAGATG AAGTTAATAC AGCAACAAAA TGACATCACT
    GGTCTTTCAC GACAAGTGAA GCATGTGATG AACTTCACTA ATTGGGCTAT TGCAAGCGGC
    AGCAGTACTG CTTTGCTCTA CAGTAAGCGG CTGATAACAT TCCAGTTACG TCATATTTTA
    AAGGCACGTT GTGATCCTGT CCCAGCTGCC AATGGAGCGA TTCGCTTCCA TTGTGACCCT
    ACATTCTGGG CAAAGAACGT CGTCAATTTA GGTAACCTTG TCATTGAAAA TAAACCAACT
    CCTACTTACA CTCCTAATGT AGTGGTTGGA CAAGCTCCTC CAGGAACAAA CCACATCAAC
    AAAGCTCCGG GACAAATTAA TCTGGCGCAG CTCCGGCTTC AGCACATGCA GCAGCAAGTT
    TATGCCCAGA AGCACCAGCA GCTGCAGCAG ATGAGGATGG GGCAGCCAAC GGGGTCCGTT
    CCCAGGCAGA GCAGCCAGCA GGTCTTGCAG CAGCAGCCTC CCAGGTTGAT CAGCATGCAG
    ACCATGCAGA GGGGTAACAT GAACTGTGGG GCTTTCCAAG CACATCAGAT GAGAATGGCT
    CAGAATGCTG CTCGTATACC AGGAATACCA CGCCACAATG GACAACAATA CTCCATGATG
    CAGCCTCACC TTCAAAGACA ACACTCTAAC CCTGGCCACG CGGGGCCTTT TCCAGTTGTC
    TCTGTGCACA ACACCACTAT TAATCCAACT AGTCCCACCA CAGCAACAAT GGCGAGTGCA
    AACCGTGGTC CAACAAGTCC GTCCGTTACA GCCATCGAGC TCATTCCTTC TGTAACAAAC
    CCAGAGAATT TACCTTCCCT GCCGGACATC CCACCGATCC AGCTTGAAGA TGCTGGTTCA
    AGTAATTTGG ATAACCTTCT AAGCAGATAC ATTACAGGCA GCCACCTACC CCCACAACCT
    ACGAGTACCA TGAATCCCTC CCCAGGACCT TCAGCTCTGT CTCCAGGGTC ATCAGGTTTA
    TCCAACTCCC ACACTCCTGT AAGGCCGCCC AGTACCTCCA GCACAGGCAG CAGAGGAAGC
    TGTGGCTCCT CGGGCAGAAC TGCTGAGAAA ACTAGCGTTA ACTTCAAGTC TGACCAAGTG
    AAAGTCAAGC AAGAGCCAGG TACAGAGGAA GAGATATGCA GTTTCTCAGG AACAGTAAAG
    CAGGAAAAAA CAGAGGATGG CAGAAGGAGT GCTTGCATGC TTAGCAGTCC TGAGAGCAGC
    TTGACACCAC CACTATCCAC TAACTTGCAT CTGGAGAGTG AATTAGAAGC ATTAGGAAGT
    CTTGAAAATC ATGTAAAAAC TGAGCCAACG GATTTAAGTG AAAGTTGCAA ACAGTCTGGA
    CACAGCCTGG TGAATGGGAA GTCCCCAGTG CGGAGTCTAA TGCACCGGTC TGCTAGGATT
    GGAGAAGGCA ATAACAAAGA TGATGATCCA AACGAGGACT GGTGCGCCGT GTGCCAAAAC
    GGAGGGGATC TCTTGTGTTG TGAAAAGTGT CCAAAAGTGT TTCACCTGAC TTGCCACGTC
    CCGACACTCC TCAGCTTTCC AAGTGGAGAG TGGATATGTA CATTCTGCAG AGATCTGAGC
    AAACCAGAAG TAGAATATGA TTGTGACAAC TCACAGCACA GCAAGAAAGG GAAAACAGCA
    CAAGGCCTGA GTCCTGTGGA CCAAAGGAAA TGTGAACGTC TCCTGCTTTA CCTGTATTGT
    CATGAGCTGA GCATTGAATT TCAAGAGCCA GTCCCAGCCT CGATACCAAA CTACTATAAA
    ATTATAAAGA AACCAATGGA CTTATCTACG GTGAAAAAGA AACTGCAAAA GAAGCATTCC
    CAACACTACC AGACTCCTGA GGATTTTGTG GCAGATGTCC GGTTGATCTT CAAGAACTGT
    GAAAGGTTTA ATGAAGCTGA TTCAGAAGTA GCGCAGGCAG GGAAGGCAGT TGCATTATAC
    TTTGAAGATA AACTTACAGA GATCTACCCA GACAGGACCT TCCAGCCTTT GCCTGAATTT
    GAGCAGGAAG AGGATGATGG TGAAATAACT GAGGACTCCG ATGAAGATTT TATACAACCA
    CGTAGAAAAC GCCTAAAATC AGATGAGAGA CCAGTGCATA TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014804033.1
    CDS28..3255
    Translation

    Target ORF information:

    RefSeq Version XM_014948547.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax tripartite motif containing 33 (TRIM33), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014948547.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGAAA ACAAAGGAGG CGGCGGCGGC GGGGAGGGGG CTGCCGAGGC CGGGCCGGGC 
    GGCGGTGGCG GCCTAGAACC TGCAGCCGCC TCTCCGTCCG GCGCCGCGCC CCCCGCGCTC
    GCCTCCGCGC CTCCCGAAGA GCGGGAGAGC CCAGGCGCGG CGGCGGCGGC GGTGGAACGA
    GGTCTCGGGG AGGCCGAGGC CGAGGCGGCG GCGGGGCCCG GTGTCGCTCC CGGGCCGGGC
    CCCAGCCCGG TGCCTCCATT GACACCGGCG GCTCCAGCGC CTTTCTCGCT GCTGGACACC
    TGCGCCGTGT GCGCGCAGAG CCTGCAGAGC CGGCGGGAGG CCGAGCCCAA GCTGCTGCCC
    TGCCTGCACT CCTTCTGCCG CCGCTGCCTG CCCGAGCCCG AGCGGCAGCT CAGCGTCCCC
    ATCCCCGGCG GCGCCAACGG CGACGTCCAG CAAGTTGGCG TGATCAGATG TCCGATATGC
    CGCCAAGAGT GCAGACAGAT AGATCTGGTG GATAACTACT TTGTAAAAGA CACGTCAGAA
    GCACCCAGCA GCTCCGATGA GAAGTCAGAG CAGGTGTGCA CAAGCTGTGA AGATAACGCC
    AGCGCTGTCG GATTTTGTGT AGAGTGTGGG GAATGGTTGT GCAAGACTTG CATAGAAGCT
    CATCAGCGAG TAAAATTTAC TAAAGATCAT ATGATCAGAA AAAAAGAGGA TGTATCTTCA
    GAGGCCGTGG GAGCATCTGG TCAACGTCCT GTTTTCTGTC CTGTCCACAA ACAAGAGCAG
    TTAAAACTTT TCTGTGAAAC CTGTGACAGG CTGACATGCA GAGACTGTCA GTTACTGGAG
    CACAAAGAAC ACAGGTATCA GTTTCTGGAA GAAGCTTTTC AGAACCAGAA GGGAGCAATT
    GAGAACCTGT TGGCCAAGCT TCTTGAGAAG AAGAACTATG TAAATTTTGC AGCTACCCAA
    GTTCAGAATA GGATAAAAGA AGTAAATGAA ACAAACAAAC GAGTAGAACA GGAAATCAAA
    GTGGCTATAT TCACCCTCAT CAATGAAATC AATAAAAAGG GAAAATCTCT CTTACAGCAC
    CTTGAGAATG TCACAAAGGA GAGACAGATG AAGTTAATAC AGCAACAAAA TGACATCACT
    GGTCTTTCAC GACAAGTGAA GCATGTGATG AACTTCACTA ATTGGGCTAT TGCAAGCGGC
    AGCAGTACTG CTTTGCTCTA CAGTAAGCGG CTGATAACAT TCCAGTTACG TCATATTTTA
    AAGGCACGTT GTGATCCTGT CCCAGCTGCC AATGGAGCGA TTCGCTTCCA TTGTGACCCT
    ACATTCTGGG CAAAGAACGT CGTCAATTTA GGTAACCTTG TCATTGAAAA TAAACCAACT
    CCTACTTACA CTCCTAATGT AGTGGTTGGA CAAGCTCCTC CAGGAACAAA CCACATCAAC
    AAAGCTCCGG GACAAATTAA TCTGGCGCAG CTCCGGCTTC AGCACATGCA GCAGCAAGTT
    TATGCCCAGA AGCACCAGCA GCTGCAGCAG ATGAGGATGG GGCAGCCAAC GGGGTCCGTT
    CCCAGGCAGA GCAGCCAGCA GGTCTTGCAG CAGCAGCCTC CCAGGTTGAT CAGCATGCAG
    ACCATGCAGA GGGGTAACAT GAACTGTGGG GCTTTCCAAG CACATCAGAT GAGAATGGCT
    CAGAATGCTG CTCGTATACC AGGAATACCA CGCCACAATG GACAACAATA CTCCATGATG
    CAGCCTCACC TTCAAAGACA ACACTCTAAC CCTGGCCACG CGGGGCCTTT TCCAGTTGTC
    TCTGTGCACA ACACCACTAT TAATCCAACT AGTCCCACCA CAGCAACAAT GGCGAGTGCA
    AACCGTGGTC CAACAAGTCC GTCCGTTACA GCCATCGAGC TCATTCCTTC TGTAACAAAC
    CCAGAGAATT TACCTTCCCT GCCGGACATC CCACCGATCC AGCTTGAAGA TGCTGGTTCA
    AGTAATTTGG ATAACCTTCT AAGCAGATAC ATTACAGGCA GCCACCTACC CCCACAACCT
    ACGAGTACCA TGAATCCCTC CCCAGGACCT TCAGCTCTGT CTCCAGGGTC ATCAGGTTTA
    TCCAACTCCC ACACTCCTGT AAGGCCGCCC AGTACCTCCA GCACAGGCAG CAGAGGAAGC
    TGTGGCTCCT CGGGCAGAAC TGCTGAGAAA ACTAGCGTTA ACTTCAAGTC TGACCAAGTG
    AAAGTCAAGC AAGAGCCAGG TACAGAGGAA GAGATATGCA GTTTCTCAGG AACAGTAAAG
    CAGGAAAAAA CAGAGGATGG CAGAAGGAGT GCTTGCATGC TTAGCAGTCC TGAGAGCAGC
    TTGACACCAC CACTATCCAC TAACTTGCAT CTGGAGAGTG AATTAGAAGC ATTAGGAAGT
    CTTGAAAATC ATGTAAAAAC TGAGCCAACG GATTTAAGTG AAAGTTGCAA ACAGTCTGGA
    CACAGCCTGG TGAATGGGAA GTCCCCAGTG CGGAGTCTAA TGCACCGGTC TGCTAGGATT
    GGAGAAGGCA ATAACAAAGA TGATGATCCA AACGAGGACT GGTGCGCCGT GTGCCAAAAC
    GGAGGGGATC TCTTGTGTTG TGAAAAGTGT CCAAAAGTGT TTCACCTGAC TTGCCACGTC
    CCGACACTCC TCAGCTTTCC AAGTGGAGAG TGGATATGTA CATTCTGCAG AGATCTGAGC
    AAACCAGAAG TAGAATATGA TTGTGACAAC TCACAGCACA GCAAGAAAGG GAAAACAGCA
    CAAGGCCTGA GTCCTGTGGA CCAAAGGAAA TGTGAACGTC TCCTGCTTTA CCTGTATTGT
    CATGAGCTGA GCATTGAATT TCAAGAGCCA GTCCCAGCCT CGATACCAAA CTACTATAAA
    ATTATAAAGA AACCAATGGA CTTATCTACG GTGAAAAAGA AACTGCAAAA GAAGCATTCC
    CAACACTACC AGACTCCTGA GGATTTTGTG GCAGATGTCC GGTTGATCTT CAAGAACTGT
    GAAAGGTTTA ATGAAGCTGA TTCAGAAGTA GCGCAGGCAG GGAAGGCAGT TGCATTATAC
    TTTGAAGATA AACTTACAGA GATCTACCCA GACAGGACCT TCCAGCCTTT GCCTGAATTT
    GAGCAGGAAG AGGATGATGG TGAAATAACT GAGGACTCCG ATGAAGATTT TATACAACCA
    CGTAGAAAAC GCCTAAAATC AGATGAGAGA CCAGTGCATA TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa69845
    Clone ID Related Accession (Same CDS sequence) XM_014948548.1
    Accession Version XM_014948548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product E3 ubiquitin-protein ligase TRIM33 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090898.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGGAAA ACAAAGGAGG CGGCGGCGGC GGGGAGGGGG CTGCCGAGGC CGGGCCGGGC 
    GGCGGTGGCG GCCTAGAACC TGCAGCCGCC TCTCCGTCCG GCGCCGCGCC CCCCGCGCTC
    GCCTCCGCGC CTCCCGAAGA GCGGGAGAGC CCAGGCGCGG CGGCGGCGGC GGTGGAACGA
    GGTCTCGGGG AGGCCGAGGC CGAGGCGGCG GCGGGGCCCG GTGTCGCTCC CGGGCCGGGC
    CCCAGCCCGG TGCCTCCATT GACACCGGCG GCTCCAGCGC CTTTCTCGCT GCTGGACACC
    TGCGCCGTGT GCGCGCAGAG CCTGCAGAGC CGGCGGGAGG CCGAGCCCAA GCTGCTGCCC
    TGCCTGCACT CCTTCTGCCG CCGCTGCCTG CCCGAGCCCG AGCGGCAGCT CAGCGTCCCC
    ATCCCCGGCG GCGCCAACGG CGACGTCCAG CAAGTTGGCG TGATCAGATG TCCGATATGC
    CGCCAAGAGT GCAGACAGAT AGATCTGGTG GATAACTACT TTGTAAAAGA CACGTCAGAA
    GCACCCAGCA GCTCCGATGA GAAGTCAGAG CAGGTGTGCA CAAGCTGTGA AGATAACGCC
    AGCGCTGTCG GATTTTGTGT AGAGTGTGGG GAATGGTTGT GCAAGACTTG CATAGAAGCT
    CATCAGCGAG TAAAATTTAC TAAAGATCAT ATGATCAGAA AAAAAGAGGA TGTATCTTCA
    GAGGCCGTGG GAGCATCTGG TCAACGTCCT GTTTTCTGTC CTGTCCACAA ACAAGAGCAG
    TTAAAACTTT TCTGTGAAAC CTGTGACAGG CTGACATGCA GAGACTGTCA GTTACTGGAG
    CACAAAGAAC ACAGGTATCA GTTTCTGGAA GAAGCTTTTC AGAACCAGAA GGGAGCAATT
    GAGAACCTGT TGGCCAAGCT TCTTGAGAAG AAGAACTATG TAAATTTTGC AGCTACCCAA
    GTTCAGAATA GGATAAAAGA AGTAAATGAA ACAAACAAAC GAGTAGAACA GGAAATCAAA
    GTGGCTATAT TCACCCTCAT CAATGAAATC AATAAAAAGG GAAAATCTCT CTTACAGCAC
    CTTGAGAATG TCACAAAGGA GAGACAGATG AAGTTAATAC AGCAACAAAA TGACATCACT
    GGTCTTTCAC GACAAGTGAA GCATGTGATG AACTTCACTA ATTGGGCTAT TGCAAGCGGC
    AGCAGTACTG CTTTGCTCTA CAGTAAGCGG CTGATAACAT TCCAGTTACG TCATATTTTA
    AAGGCACGTT GTGATCCTGT CCCAGCTGCC AATGGAGCGA TTCGCTTCCA TTGTGACCCT
    ACATTCTGGG CAAAGAACGT CGTCAATTTA GGTAACCTTG TCATTGAAAA TAAACCAACT
    CCTACTTACA CTCCTAATGT AGTGGTTGGA CAAGCTCCTC CAGGAACAAA CCACATCAAC
    AAAGCTCCGG GACAAATTAA TCTGGCGCAG CTCCGGCTTC AGCACATGCA GCAGCAAGTT
    TATGCCCAGA AGCACCAGCA GCTGCAGCAG ATGAGGATGG GGCAGCCAAC GGGGTCCGTT
    CCCAGGCAGA GCAGCCAGCA GGTCTTGCAG CAGCAGCACT CTAACCCTGG CCACGCGGGG
    CCTTTTCCAG TTGTCTCTGT GCACAACACC ACTATTAATC CAACTAGTCC CACCACAGCA
    ACAATGGCGA GTGCAAACCG TGGTCCAACA AGTCCGTCCG TTACAGCCAT CGAGCTCATT
    CCTTCTGTAA CAAACCCAGA GAATTTACCT TCCCTGCCGG ACATCCCACC GATCCAGCTT
    GAAGATGCTG GTTCAAGTAA TTTGGATAAC CTTCTAAGCA GATACATTAC AGGCAGCCAC
    CTACCCCCAC AACCTACGAG TACCATGAAT CCCTCCCCAG GACCTTCAGC TCTGTCTCCA
    GGGTCATCAG GTTTATCCAA CTCCCACACT CCTGTAAGGC CGCCCAGTAC CTCCAGCACA
    GGCAGCAGAG GAAGCTGTGG CTCCTCGGGC AGAACTGCTG AGAAAACTAG CGTTAACTTC
    AAGTCTGACC AAGTGAAAGT CAAGCAAGAG CCAGGTACAG AGGAAGAGAT ATGCAGTTTC
    TCAGGAACAG TAAAGCAGGA AAAAACAGAG GATGGCAGAA GGAGTGCTTG CATGCTTAGC
    AGTCCTGAGA GCAGCTTGAC ACCACCACTA TCCACTAACT TGCATCTGGA GAGTGAATTA
    GAAGCATTAG GAAGTCTTGA AAATCATGTA AAAACTGAGC CAACGGATTT AAGTGAAAGT
    TGCAAACAGT CTGGACACAG CCTGGTGAAT GGGAAGTCCC CAGTGCGGAG TCTAATGCAC
    CGGTCTGCTA GGATTGGAGA AGGCAATAAC AAAGATGATG ATCCAAACGA GGACTGGTGC
    GCCGTGTGCC AAAACGGAGG GGATCTCTTG TGTTGTGAAA AGTGTCCAAA AGTGTTTCAC
    CTGACTTGCC ACGTCCCGAC ACTCCTCAGC TTTCCAAGTG GAGAGTGGAT ATGTACATTC
    TGCAGAGATC TGAGCAAACC AGAAGTAGAA TATGATTGTG ACAACTCACA GCACAGCAAG
    AAAGGGAAAA CAGCACAAGG CCTGAGTCCT GTGGACCAAA GGAAATGTGA ACGTCTCCTG
    CTTTACCTGT ATTGTCATGA GCTGAGCATT GAATTTCAAG AGCCAGTCCC AGCCTCGATA
    CCAAACTACT ATAAAATTAT AAAGAAACCA ATGGACTTAT CTACGGTGAA AAAGAAACTG
    CAAAAGAAGC ATTCCCAACA CTACCAGACT CCTGAGGATT TTGTGGCAGA TGTCCGGTTG
    ATCTTCAAGA ACTGTGAAAG GTTTAATGAA ATGATGAAAG TTGTTCAAGT TTATGCAGAA
    ACACAAGAGA TTAATTTGAA GGCTGATTCA GAAGTAGCGC AGGCAGGGAA GGCAGTTGCA
    TTATACTTTG AAGATAAACT TACAGAGATC TACCCAGACA GGACCTTCCA GCCTTTGCCT
    GAATTTGAGC AGGAAGAGGA TGATGGTGAA ATAACTGAGG ACTCCGATGA AGATTTTATA
    CAACCACGTA GAAAACGCCT AAAATCAGAT GAGAGACCAG TGCATATAAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014804034.1
    CDS28..3141
    Translation

    Target ORF information:

    RefSeq Version XM_014948548.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax tripartite motif containing 33 (TRIM33), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014948548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGGAAA ACAAAGGAGG CGGCGGCGGC GGGGAGGGGG CTGCCGAGGC CGGGCCGGGC 
    GGCGGTGGCG GCCTAGAACC TGCAGCCGCC TCTCCGTCCG GCGCCGCGCC CCCCGCGCTC
    GCCTCCGCGC CTCCCGAAGA GCGGGAGAGC CCAGGCGCGG CGGCGGCGGC GGTGGAACGA
    GGTCTCGGGG AGGCCGAGGC CGAGGCGGCG GCGGGGCCCG GTGTCGCTCC CGGGCCGGGC
    CCCAGCCCGG TGCCTCCATT GACACCGGCG GCTCCAGCGC CTTTCTCGCT GCTGGACACC
    TGCGCCGTGT GCGCGCAGAG CCTGCAGAGC CGGCGGGAGG CCGAGCCCAA GCTGCTGCCC
    TGCCTGCACT CCTTCTGCCG CCGCTGCCTG CCCGAGCCCG AGCGGCAGCT CAGCGTCCCC
    ATCCCCGGCG GCGCCAACGG CGACGTCCAG CAAGTTGGCG TGATCAGATG TCCGATATGC
    CGCCAAGAGT GCAGACAGAT AGATCTGGTG GATAACTACT TTGTAAAAGA CACGTCAGAA
    GCACCCAGCA GCTCCGATGA GAAGTCAGAG CAGGTGTGCA CAAGCTGTGA AGATAACGCC
    AGCGCTGTCG GATTTTGTGT AGAGTGTGGG GAATGGTTGT GCAAGACTTG CATAGAAGCT
    CATCAGCGAG TAAAATTTAC TAAAGATCAT ATGATCAGAA AAAAAGAGGA TGTATCTTCA
    GAGGCCGTGG GAGCATCTGG TCAACGTCCT GTTTTCTGTC CTGTCCACAA ACAAGAGCAG
    TTAAAACTTT TCTGTGAAAC CTGTGACAGG CTGACATGCA GAGACTGTCA GTTACTGGAG
    CACAAAGAAC ACAGGTATCA GTTTCTGGAA GAAGCTTTTC AGAACCAGAA GGGAGCAATT
    GAGAACCTGT TGGCCAAGCT TCTTGAGAAG AAGAACTATG TAAATTTTGC AGCTACCCAA
    GTTCAGAATA GGATAAAAGA AGTAAATGAA ACAAACAAAC GAGTAGAACA GGAAATCAAA
    GTGGCTATAT TCACCCTCAT CAATGAAATC AATAAAAAGG GAAAATCTCT CTTACAGCAC
    CTTGAGAATG TCACAAAGGA GAGACAGATG AAGTTAATAC AGCAACAAAA TGACATCACT
    GGTCTTTCAC GACAAGTGAA GCATGTGATG AACTTCACTA ATTGGGCTAT TGCAAGCGGC
    AGCAGTACTG CTTTGCTCTA CAGTAAGCGG CTGATAACAT TCCAGTTACG TCATATTTTA
    AAGGCACGTT GTGATCCTGT CCCAGCTGCC AATGGAGCGA TTCGCTTCCA TTGTGACCCT
    ACATTCTGGG CAAAGAACGT CGTCAATTTA GGTAACCTTG TCATTGAAAA TAAACCAACT
    CCTACTTACA CTCCTAATGT AGTGGTTGGA CAAGCTCCTC CAGGAACAAA CCACATCAAC
    AAAGCTCCGG GACAAATTAA TCTGGCGCAG CTCCGGCTTC AGCACATGCA GCAGCAAGTT
    TATGCCCAGA AGCACCAGCA GCTGCAGCAG ATGAGGATGG GGCAGCCAAC GGGGTCCGTT
    CCCAGGCAGA GCAGCCAGCA GGTCTTGCAG CAGCAGCACT CTAACCCTGG CCACGCGGGG
    CCTTTTCCAG TTGTCTCTGT GCACAACACC ACTATTAATC CAACTAGTCC CACCACAGCA
    ACAATGGCGA GTGCAAACCG TGGTCCAACA AGTCCGTCCG TTACAGCCAT CGAGCTCATT
    CCTTCTGTAA CAAACCCAGA GAATTTACCT TCCCTGCCGG ACATCCCACC GATCCAGCTT
    GAAGATGCTG GTTCAAGTAA TTTGGATAAC CTTCTAAGCA GATACATTAC AGGCAGCCAC
    CTACCCCCAC AACCTACGAG TACCATGAAT CCCTCCCCAG GACCTTCAGC TCTGTCTCCA
    GGGTCATCAG GTTTATCCAA CTCCCACACT CCTGTAAGGC CGCCCAGTAC CTCCAGCACA
    GGCAGCAGAG GAAGCTGTGG CTCCTCGGGC AGAACTGCTG AGAAAACTAG CGTTAACTTC
    AAGTCTGACC AAGTGAAAGT CAAGCAAGAG CCAGGTACAG AGGAAGAGAT ATGCAGTTTC
    TCAGGAACAG TAAAGCAGGA AAAAACAGAG GATGGCAGAA GGAGTGCTTG CATGCTTAGC
    AGTCCTGAGA GCAGCTTGAC ACCACCACTA TCCACTAACT TGCATCTGGA GAGTGAATTA
    GAAGCATTAG GAAGTCTTGA AAATCATGTA AAAACTGAGC CAACGGATTT AAGTGAAAGT
    TGCAAACAGT CTGGACACAG CCTGGTGAAT GGGAAGTCCC CAGTGCGGAG TCTAATGCAC
    CGGTCTGCTA GGATTGGAGA AGGCAATAAC AAAGATGATG ATCCAAACGA GGACTGGTGC
    GCCGTGTGCC AAAACGGAGG GGATCTCTTG TGTTGTGAAA AGTGTCCAAA AGTGTTTCAC
    CTGACTTGCC ACGTCCCGAC ACTCCTCAGC TTTCCAAGTG GAGAGTGGAT ATGTACATTC
    TGCAGAGATC TGAGCAAACC AGAAGTAGAA TATGATTGTG ACAACTCACA GCACAGCAAG
    AAAGGGAAAA CAGCACAAGG CCTGAGTCCT GTGGACCAAA GGAAATGTGA ACGTCTCCTG
    CTTTACCTGT ATTGTCATGA GCTGAGCATT GAATTTCAAG AGCCAGTCCC AGCCTCGATA
    CCAAACTACT ATAAAATTAT AAAGAAACCA ATGGACTTAT CTACGGTGAA AAAGAAACTG
    CAAAAGAAGC ATTCCCAACA CTACCAGACT CCTGAGGATT TTGTGGCAGA TGTCCGGTTG
    ATCTTCAAGA ACTGTGAAAG GTTTAATGAA ATGATGAAAG TTGTTCAAGT TTATGCAGAA
    ACACAAGAGA TTAATTTGAA GGCTGATTCA GAAGTAGCGC AGGCAGGGAA GGCAGTTGCA
    TTATACTTTG AAGATAAACT TACAGAGATC TACCCAGACA GGACCTTCCA GCCTTTGCCT
    GAATTTGAGC AGGAAGAGGA TGATGGTGAA ATAACTGAGG ACTCCGATGA AGATTTTATA
    CAACCACGTA GAAAACGCCT AAAATCAGAT GAGAGACCAG TGCATATAAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.