SEC31A cDNA ORF clone, Calidris pugnax(ruff)

The following SEC31A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC31A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa70923 XM_014949993.1
Latest version!
Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa70924 XM_014949994.1
Latest version!
Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa70925 XM_014949995.1
Latest version!
Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa70926 XM_014949996.1
Latest version!
Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa70927 XM_014949997.1
Latest version!
Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa70928 XM_014949998.1
Latest version!
Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa70929 XM_014949999.1
Latest version!
Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa70930 XM_014950000.1
Latest version!
Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa70931 XM_014950001.1
Latest version!
Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa70923
    Clone ID Related Accession (Same CDS sequence) XM_014949993.1
    Accession Version XM_014949993.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product protein transport protein Sec31A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGGTAC TCTGCCTGCA
    GCCAGCGACC TTCCTGCATC CCAGAGAACA GAAAGCCTTC CAACTCGAGA CCAGGCCTCT
    CCCTTCCCAG GTGAACTAGG GCCTCAGAAT GGCTGGAACG ACCCTCCCGC CCTGAACAGA
    GCTGCCAAGA AGAAGAAGGT CCCTGATAAC TTCCTGCCCC CAGTGCCCAT CACCTCCCCC
    ATCATGAACC CGCTGGCGGA TCCGCAGGCT CAGATGCAGC AGCCCCCGGC TCCGGCAGCC
    CCCGTGGGTG CACCCAGCTT CCAGCCTCCC CACCTCCCCG TGGGGCAGGG GGGCTACCCA
    CCTGCTCCCC ACACCCTGGG ACCCTGCATC GTGCCGCCTG CCGTTGCCAA ACCCAGCACA
    GAGGGGGCCC CGGGGGCCCC AATTGGCAAC GCCATCCAGC ATGTCCAGGC ACTGCCGACA
    GAGAAGATTA CCAAGAAGCC CATCCCTGAG GAGCACCTTA TTCTGAAGAC TACATTTGAA
    GCTCTTATCC AGAGGTGCCT GCTGTCTGCT TCCGATCCGC AAACCAAGAG GAAACTAGAT
    GATGCAAACA AACGCCTGGA GTTTCTATAT GACAAACTGA GGGAGCAGAC ACTCTCTCCC
    ACCATCGTGA GTGGTTTGCA CAACATGGTG AAGAGCATCG AAACCCGCAA CTACCAGGAG
    GGGCTGAACA TCCACACGCA CATCGTCAGC ACCAGCAACT TCAGCGAGAC CTCCGCCTTC
    ATGCCCGTGC TGAAGGTTGT CCTCACCCAG GCCAACAAGC TGGGTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805479.1
    CDS462..4292
    Translation

    Target ORF information:

    RefSeq Version XM_014949993.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949993.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGGTAC TCTGCCTGCA
    GCCAGCGACC TTCCTGCATC CCAGAGAACA GAAAGCCTTC CAACTCGAGA CCAGGCCTCT
    CCCTTCCCAG GTGAACTAGG GCCTCAGAAT GGCTGGAACG ACCCTCCCGC CCTGAACAGA
    GCTGCCAAGA AGAAGAAGGT CCCTGATAAC TTCCTGCCCC CAGTGCCCAT CACCTCCCCC
    ATCATGAACC CGCTGGCGGA TCCGCAGGCT CAGATGCAGC AGCCCCCGGC TCCGGCAGCC
    CCCGTGGGTG CACCCAGCTT CCAGCCTCCC CACCTCCCCG TGGGGCAGGG GGGCTACCCA
    CCTGCTCCCC ACACCCTGGG ACCCTGCATC GTGCCGCCTG CCGTTGCCAA ACCCAGCACA
    GAGGGGGCCC CGGGGGCCCC AATTGGCAAC GCCATCCAGC ATGTCCAGGC ACTGCCGACA
    GAGAAGATTA CCAAGAAGCC CATCCCTGAG GAGCACCTTA TTCTGAAGAC TACATTTGAA
    GCTCTTATCC AGAGGTGCCT GCTGTCTGCT TCCGATCCGC AAACCAAGAG GAAACTAGAT
    GATGCAAACA AACGCCTGGA GTTTCTATAT GACAAACTGA GGGAGCAGAC ACTCTCTCCC
    ACCATCGTGA GTGGTTTGCA CAACATGGTG AAGAGCATCG AAACCCGCAA CTACCAGGAG
    GGGCTGAACA TCCACACGCA CATCGTCAGC ACCAGCAACT TCAGCGAGAC CTCCGCCTTC
    ATGCCCGTGC TGAAGGTTGT CCTCACCCAG GCCAACAAGC TGGGTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70924
    Clone ID Related Accession (Same CDS sequence) XM_014949994.1
    Accession Version XM_014949994.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product protein transport protein Sec31A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGGTAC TCTGCCTGCA
    GCCAGCGACC TTCCTGCATC CCAGAGAACA GAAAGCCTTC CAACTCGAGA CCAGGCCTCT
    CCCTTCCCAG GGCCTCAGAA TGGCTGGAAC GACCCTCCCG CCCTGAACAG AGCTGCCAAG
    AAGAAGAAGG TCCCTGATAA CTTCCTGCCC CCAGTGCCCA TCACCTCCCC CATCATGAAC
    CCGCTGGCGG ATCCGCAGGC TCAGATGCAG CAGCCCCCGG CTCCGGCAGC CCCCGTGGGT
    GCACCCAGCT TCCAGCCTCC CCACCTCCCC GTGGGGCAGG GGGGCTACCC ACCTGCTCCC
    CACACCCTGG GACCCTGCAT CGTGCCGCCT GCCGTTGCCA AACCCAGCAC AGAGGGGGCC
    CCGGGGGCCC CAATTGGCAA CGCCATCCAG CATGTCCAGG CACTGCCGAC AGAGAAGATT
    ACCAAGAAGC CCATCCCTGA GGAGCACCTT ATTCTGAAGA CTACATTTGA AGCTCTTATC
    CAGAGGTGCC TGCTGTCTGC TTCCGATCCG CAAACCAAGA GGAAACTAGA TGATGCAAAC
    AAACGCCTGG AGTTTCTATA TGACAAACTG AGGGAGCAGA CACTCTCTCC CACCATCGTG
    AGTGGTTTGC ACAACATGGT GAAGAGCATC GAAACCCGCA ACTACCAGGA GGGGCTGAAC
    ATCCACACGC ACATCGTCAG CACCAGCAAC TTCAGCGAGA CCTCCGCCTT CATGCCCGTG
    CTGAAGGTTG TCCTCACCCA GGCCAACAAG CTGGGTGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805480.1
    CDS462..4283
    Translation

    Target ORF information:

    RefSeq Version XM_014949994.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949994.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGGTAC TCTGCCTGCA
    GCCAGCGACC TTCCTGCATC CCAGAGAACA GAAAGCCTTC CAACTCGAGA CCAGGCCTCT
    CCCTTCCCAG GGCCTCAGAA TGGCTGGAAC GACCCTCCCG CCCTGAACAG AGCTGCCAAG
    AAGAAGAAGG TCCCTGATAA CTTCCTGCCC CCAGTGCCCA TCACCTCCCC CATCATGAAC
    CCGCTGGCGG ATCCGCAGGC TCAGATGCAG CAGCCCCCGG CTCCGGCAGC CCCCGTGGGT
    GCACCCAGCT TCCAGCCTCC CCACCTCCCC GTGGGGCAGG GGGGCTACCC ACCTGCTCCC
    CACACCCTGG GACCCTGCAT CGTGCCGCCT GCCGTTGCCA AACCCAGCAC AGAGGGGGCC
    CCGGGGGCCC CAATTGGCAA CGCCATCCAG CATGTCCAGG CACTGCCGAC AGAGAAGATT
    ACCAAGAAGC CCATCCCTGA GGAGCACCTT ATTCTGAAGA CTACATTTGA AGCTCTTATC
    CAGAGGTGCC TGCTGTCTGC TTCCGATCCG CAAACCAAGA GGAAACTAGA TGATGCAAAC
    AAACGCCTGG AGTTTCTATA TGACAAACTG AGGGAGCAGA CACTCTCTCC CACCATCGTG
    AGTGGTTTGC ACAACATGGT GAAGAGCATC GAAACCCGCA ACTACCAGGA GGGGCTGAAC
    ATCCACACGC ACATCGTCAG CACCAGCAAC TTCAGCGAGA CCTCCGCCTT CATGCCCGTG
    CTGAAGGTTG TCCTCACCCA GGCCAACAAG CTGGGTGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70925
    Clone ID Related Accession (Same CDS sequence) XM_014949995.1
    Accession Version XM_014949995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product protein transport protein Sec31A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGAAAG CCTTCCAACT
    CGAGACCAGG CCTCTCCCTT CCCAGGTGAA CTAGGGCCTC AGAATGGCTG GAACGACCCT
    CCCGCCCTGA ACAGAGCTGC CAAGAAGAAG AAGGTCCCTG ATAACTTCCT GCCCCCAGTG
    CCCATCACCT CCCCCATCAT GAACCCGCTG GCGGATCCGC AGGCTCAGAT GCAGCAGCCC
    CCGGCTCCGG CAGCCCCCGT GGGTGCACCC AGCTTCCAGC CTCCCCACCT CCCCGTGGGG
    CAGGGGGGCT ACCCACCTGC TCCCCACACC CTGGGACCCT GCATCGTGCC GCCTGCCGTT
    GCCAAACCCA GCACAGAGGG GGCCCCGGGG GCCCCAATTG GCAACGCCAT CCAGCATGTC
    CAGGCACTGC CGACAGAGAA GATTACCAAG AAGCCCATCC CTGAGGAGCA CCTTATTCTG
    AAGACTACAT TTGAAGCTCT TATCCAGAGG TGCCTGCTGT CTGCTTCCGA TCCGCAAACC
    AAGAGGAAAC TAGATGATGC AAACAAACGC CTGGAGTTTC TATATGACAA ACTGAGGGAG
    CAGACACTCT CTCCCACCAT CGTGAGTGGT TTGCACAACA TGGTGAAGAG CATCGAAACC
    CGCAACTACC AGGAGGGGCT GAACATCCAC ACGCACATCG TCAGCACCAG CAACTTCAGC
    GAGACCTCCG CCTTCATGCC CGTGCTGAAG GTTGTCCTCA CCCAGGCCAA CAAGCTGGGT
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805481.1
    CDS462..4247
    Translation

    Target ORF information:

    RefSeq Version XM_014949995.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGAAAG CCTTCCAACT
    CGAGACCAGG CCTCTCCCTT CCCAGGTGAA CTAGGGCCTC AGAATGGCTG GAACGACCCT
    CCCGCCCTGA ACAGAGCTGC CAAGAAGAAG AAGGTCCCTG ATAACTTCCT GCCCCCAGTG
    CCCATCACCT CCCCCATCAT GAACCCGCTG GCGGATCCGC AGGCTCAGAT GCAGCAGCCC
    CCGGCTCCGG CAGCCCCCGT GGGTGCACCC AGCTTCCAGC CTCCCCACCT CCCCGTGGGG
    CAGGGGGGCT ACCCACCTGC TCCCCACACC CTGGGACCCT GCATCGTGCC GCCTGCCGTT
    GCCAAACCCA GCACAGAGGG GGCCCCGGGG GCCCCAATTG GCAACGCCAT CCAGCATGTC
    CAGGCACTGC CGACAGAGAA GATTACCAAG AAGCCCATCC CTGAGGAGCA CCTTATTCTG
    AAGACTACAT TTGAAGCTCT TATCCAGAGG TGCCTGCTGT CTGCTTCCGA TCCGCAAACC
    AAGAGGAAAC TAGATGATGC AAACAAACGC CTGGAGTTTC TATATGACAA ACTGAGGGAG
    CAGACACTCT CTCCCACCAT CGTGAGTGGT TTGCACAACA TGGTGAAGAG CATCGAAACC
    CGCAACTACC AGGAGGGGCT GAACATCCAC ACGCACATCG TCAGCACCAG CAACTTCAGC
    GAGACCTCCG CCTTCATGCC CGTGCTGAAG GTTGTCCTCA CCCAGGCCAA CAAGCTGGGT
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70926
    Clone ID Related Accession (Same CDS sequence) XM_014949996.1
    Accession Version XM_014949996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product protein transport protein Sec31A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGGTAC TCTGCCTGCA
    GCCAGCGACC TTCCTGCATC CCAGAGAACA GGGCCTCAGA ATGGCTGGAA CGACCCTCCC
    GCCCTGAACA GAGCTGCCAA GAAGAAGAAG GTCCCTGATA ACTTCCTGCC CCCAGTGCCC
    ATCACCTCCC CCATCATGAA CCCGCTGGCG GATCCGCAGG CTCAGATGCA GCAGCCCCCG
    GCTCCGGCAG CCCCCGTGGG TGCACCCAGC TTCCAGCCTC CCCACCTCCC CGTGGGGCAG
    GGGGGCTACC CACCTGCTCC CCACACCCTG GGACCCTGCA TCGTGCCGCC TGCCGTTGCC
    AAACCCAGCA CAGAGGGGGC CCCGGGGGCC CCAATTGGCA ACGCCATCCA GCATGTCCAG
    GCACTGCCGA CAGAGAAGAT TACCAAGAAG CCCATCCCTG AGGAGCACCT TATTCTGAAG
    ACTACATTTG AAGCTCTTAT CCAGAGGTGC CTGCTGTCTG CTTCCGATCC GCAAACCAAG
    AGGAAACTAG ATGATGCAAA CAAACGCCTG GAGTTTCTAT ATGACAAACT GAGGGAGCAG
    ACACTCTCTC CCACCATCGT GAGTGGTTTG CACAACATGG TGAAGAGCAT CGAAACCCGC
    AACTACCAGG AGGGGCTGAA CATCCACACG CACATCGTCA GCACCAGCAA CTTCAGCGAG
    ACCTCCGCCT TCATGCCCGT GCTGAAGGTT GTCCTCACCC AGGCCAACAA GCTGGGTGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805482.1
    CDS462..4244
    Translation

    Target ORF information:

    RefSeq Version XM_014949996.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGGTAC TCTGCCTGCA
    GCCAGCGACC TTCCTGCATC CCAGAGAACA GGGCCTCAGA ATGGCTGGAA CGACCCTCCC
    GCCCTGAACA GAGCTGCCAA GAAGAAGAAG GTCCCTGATA ACTTCCTGCC CCCAGTGCCC
    ATCACCTCCC CCATCATGAA CCCGCTGGCG GATCCGCAGG CTCAGATGCA GCAGCCCCCG
    GCTCCGGCAG CCCCCGTGGG TGCACCCAGC TTCCAGCCTC CCCACCTCCC CGTGGGGCAG
    GGGGGCTACC CACCTGCTCC CCACACCCTG GGACCCTGCA TCGTGCCGCC TGCCGTTGCC
    AAACCCAGCA CAGAGGGGGC CCCGGGGGCC CCAATTGGCA ACGCCATCCA GCATGTCCAG
    GCACTGCCGA CAGAGAAGAT TACCAAGAAG CCCATCCCTG AGGAGCACCT TATTCTGAAG
    ACTACATTTG AAGCTCTTAT CCAGAGGTGC CTGCTGTCTG CTTCCGATCC GCAAACCAAG
    AGGAAACTAG ATGATGCAAA CAAACGCCTG GAGTTTCTAT ATGACAAACT GAGGGAGCAG
    ACACTCTCTC CCACCATCGT GAGTGGTTTG CACAACATGG TGAAGAGCAT CGAAACCCGC
    AACTACCAGG AGGGGCTGAA CATCCACACG CACATCGTCA GCACCAGCAA CTTCAGCGAG
    ACCTCCGCCT TCATGCCCGT GCTGAAGGTT GTCCTCACCC AGGCCAACAA GCTGGGTGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70927
    Clone ID Related Accession (Same CDS sequence) XM_014949997.1
    Accession Version XM_014949997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product protein transport protein Sec31A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGAAAG CCTTCCAACT
    CGAGACCAGG CCTCTCCCTT CCCAGGGCCT CAGAATGGCT GGAACGACCC TCCCGCCCTG
    AACAGAGCTG CCAAGAAGAA GAAGGTCCCT GATAACTTCC TGCCCCCAGT GCCCATCACC
    TCCCCCATCA TGAACCCGCT GGCGGATCCG CAGGCTCAGA TGCAGCAGCC CCCGGCTCCG
    GCAGCCCCCG TGGGTGCACC CAGCTTCCAG CCTCCCCACC TCCCCGTGGG GCAGGGGGGC
    TACCCACCTG CTCCCCACAC CCTGGGACCC TGCATCGTGC CGCCTGCCGT TGCCAAACCC
    AGCACAGAGG GGGCCCCGGG GGCCCCAATT GGCAACGCCA TCCAGCATGT CCAGGCACTG
    CCGACAGAGA AGATTACCAA GAAGCCCATC CCTGAGGAGC ACCTTATTCT GAAGACTACA
    TTTGAAGCTC TTATCCAGAG GTGCCTGCTG TCTGCTTCCG ATCCGCAAAC CAAGAGGAAA
    CTAGATGATG CAAACAAACG CCTGGAGTTT CTATATGACA AACTGAGGGA GCAGACACTC
    TCTCCCACCA TCGTGAGTGG TTTGCACAAC ATGGTGAAGA GCATCGAAAC CCGCAACTAC
    CAGGAGGGGC TGAACATCCA CACGCACATC GTCAGCACCA GCAACTTCAG CGAGACCTCC
    GCCTTCATGC CCGTGCTGAA GGTTGTCCTC ACCCAGGCCA ACAAGCTGGG TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805483.1
    CDS462..4238
    Translation

    Target ORF information:

    RefSeq Version XM_014949997.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGAAAG CCTTCCAACT
    CGAGACCAGG CCTCTCCCTT CCCAGGGCCT CAGAATGGCT GGAACGACCC TCCCGCCCTG
    AACAGAGCTG CCAAGAAGAA GAAGGTCCCT GATAACTTCC TGCCCCCAGT GCCCATCACC
    TCCCCCATCA TGAACCCGCT GGCGGATCCG CAGGCTCAGA TGCAGCAGCC CCCGGCTCCG
    GCAGCCCCCG TGGGTGCACC CAGCTTCCAG CCTCCCCACC TCCCCGTGGG GCAGGGGGGC
    TACCCACCTG CTCCCCACAC CCTGGGACCC TGCATCGTGC CGCCTGCCGT TGCCAAACCC
    AGCACAGAGG GGGCCCCGGG GGCCCCAATT GGCAACGCCA TCCAGCATGT CCAGGCACTG
    CCGACAGAGA AGATTACCAA GAAGCCCATC CCTGAGGAGC ACCTTATTCT GAAGACTACA
    TTTGAAGCTC TTATCCAGAG GTGCCTGCTG TCTGCTTCCG ATCCGCAAAC CAAGAGGAAA
    CTAGATGATG CAAACAAACG CCTGGAGTTT CTATATGACA AACTGAGGGA GCAGACACTC
    TCTCCCACCA TCGTGAGTGG TTTGCACAAC ATGGTGAAGA GCATCGAAAC CCGCAACTAC
    CAGGAGGGGC TGAACATCCA CACGCACATC GTCAGCACCA GCAACTTCAG CGAGACCTCC
    GCCTTCATGC CCGTGCTGAA GGTTGTCCTC ACCCAGGCCA ACAAGCTGGG TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70928
    Clone ID Related Accession (Same CDS sequence) XM_014949998.1
    Accession Version XM_014949998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product protein transport protein Sec31A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGGAGAC
    AACCCACCTC TTCCAGGCTT CATCCTGCCG GGTGCCGTTA ACCCCAACGT GCCGTCGCAG
    CAAGCCGCGT CTTCCAATTG CGTCATGTAC CCACCAGCTG GGGCCCGACC AACCTACCTG
    CAGACGTATC AGGCCACACA GCAGTACCCT TGTGGAACGG GGGGACCAGC TCTCTATCAG
    CCTCAGCAGC CTCTCGCTGT CCCTGCTTCA GCCTCCTACC CAAACCCTGC CCCTAACACC
    ACCCCTCCCT ACCCCTCTGC CTCTGCCCAC CCCATGCCCT CCCCGCTGTA CCCCGGGCAG
    CCTCAGCCCT CCCCAACAAG CCTGAATCCC AGCTCTTCCT TCCCTCTTCC TCCTTCTGGC
    ACGTCCTTTC AGCATGGCAG GCCAGGACCT CCAGCAACTT CTGTGGCTTA TGCCTTGCCT
    ACGGGACCAA CAGGTACTCT GCCTGCAGCC AGCGACCTTC CTGCATCCCA GAGAACAGAA
    AGCCTTCCAA CTCGAGACCA GGCCTCTCCC TTCCCAGGTG AACTAGGGCC TCAGAATGGC
    TGGAACGACC CTCCCGCCCT GAACAGAGCT GCCAAGAAGA AGAAGGTCCC TGATAACTTC
    CTGCCCCCAG TGCCCATCAC CTCCCCCATC ATGAACCCGC TGGCGGATCC GCAGGCTCAG
    ATGCAGCAGC CCCCGGCTCC GGCAGCCCCC GTGGGTGCAC CCAGCTTCCA GCCTCCCCAC
    CTCCCCGTGG GGCAGGGGGG CTACCCACCT GCTCCCCACA CCCTGGGACC CTGCATCGTG
    CCGCCTGCCG TTGCCAAACC CAGCACAGAG GGGGCCCCGG GGGCCCCAAT TGGCAACGCC
    ATCCAGCATG TCCAGGCACT GCCGACAGAG AAGATTACCA AGAAGCCCAT CCCTGAGGAG
    CACCTTATTC TGAAGACTAC ATTTGAAGCT CTTATCCAGA GGTGCCTGCT GTCTGCTTCC
    GATCCGCAAA CCAAGAGGAA ACTAGATGAT GCAAACAAAC GCCTGGAGTT TCTATATGAC
    AAACTGAGGG AGCAGACACT CTCTCCCACC ATCGTGAGTG GTTTGCACAA CATGGTGAAG
    AGCATCGAAA CCCGCAACTA CCAGGAGGGG CTGAACATCC ACACGCACAT CGTCAGCACC
    AGCAACTTCA GCGAGACCTC CGCCTTCATG CCCGTGCTGA AGGTTGTCCT CACCCAGGCC
    AACAAGCTGG GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805484.1
    CDS462..4199
    Translation

    Target ORF information:

    RefSeq Version XM_014949998.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGGAGAC
    AACCCACCTC TTCCAGGCTT CATCCTGCCG GGTGCCGTTA ACCCCAACGT GCCGTCGCAG
    CAAGCCGCGT CTTCCAATTG CGTCATGTAC CCACCAGCTG GGGCCCGACC AACCTACCTG
    CAGACGTATC AGGCCACACA GCAGTACCCT TGTGGAACGG GGGGACCAGC TCTCTATCAG
    CCTCAGCAGC CTCTCGCTGT CCCTGCTTCA GCCTCCTACC CAAACCCTGC CCCTAACACC
    ACCCCTCCCT ACCCCTCTGC CTCTGCCCAC CCCATGCCCT CCCCGCTGTA CCCCGGGCAG
    CCTCAGCCCT CCCCAACAAG CCTGAATCCC AGCTCTTCCT TCCCTCTTCC TCCTTCTGGC
    ACGTCCTTTC AGCATGGCAG GCCAGGACCT CCAGCAACTT CTGTGGCTTA TGCCTTGCCT
    ACGGGACCAA CAGGTACTCT GCCTGCAGCC AGCGACCTTC CTGCATCCCA GAGAACAGAA
    AGCCTTCCAA CTCGAGACCA GGCCTCTCCC TTCCCAGGTG AACTAGGGCC TCAGAATGGC
    TGGAACGACC CTCCCGCCCT GAACAGAGCT GCCAAGAAGA AGAAGGTCCC TGATAACTTC
    CTGCCCCCAG TGCCCATCAC CTCCCCCATC ATGAACCCGC TGGCGGATCC GCAGGCTCAG
    ATGCAGCAGC CCCCGGCTCC GGCAGCCCCC GTGGGTGCAC CCAGCTTCCA GCCTCCCCAC
    CTCCCCGTGG GGCAGGGGGG CTACCCACCT GCTCCCCACA CCCTGGGACC CTGCATCGTG
    CCGCCTGCCG TTGCCAAACC CAGCACAGAG GGGGCCCCGG GGGCCCCAAT TGGCAACGCC
    ATCCAGCATG TCCAGGCACT GCCGACAGAG AAGATTACCA AGAAGCCCAT CCCTGAGGAG
    CACCTTATTC TGAAGACTAC ATTTGAAGCT CTTATCCAGA GGTGCCTGCT GTCTGCTTCC
    GATCCGCAAA CCAAGAGGAA ACTAGATGAT GCAAACAAAC GCCTGGAGTT TCTATATGAC
    AAACTGAGGG AGCAGACACT CTCTCCCACC ATCGTGAGTG GTTTGCACAA CATGGTGAAG
    AGCATCGAAA CCCGCAACTA CCAGGAGGGG CTGAACATCC ACACGCACAT CGTCAGCACC
    AGCAACTTCA GCGAGACCTC CGCCTTCATG CCCGTGCTGA AGGTTGTCCT CACCCAGGCC
    AACAAGCTGG GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70929
    Clone ID Related Accession (Same CDS sequence) XM_014949999.1
    Accession Version XM_014949999.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product protein transport protein Sec31A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGGGCC TCAGAATGGC
    TGGAACGACC CTCCCGCCCT GAACAGAGCT GCCAAGAAGA AGAAGGTCCC TGATAACTTC
    CTGCCCCCAG TGCCCATCAC CTCCCCCATC ATGAACCCGC TGGCGGATCC GCAGGCTCAG
    ATGCAGCAGC CCCCGGCTCC GGCAGCCCCC GTGGGTGCAC CCAGCTTCCA GCCTCCCCAC
    CTCCCCGTGG GGCAGGGGGG CTACCCACCT GCTCCCCACA CCCTGGGACC CTGCATCGTG
    CCGCCTGCCG TTGCCAAACC CAGCACAGAG GGGGCCCCGG GGGCCCCAAT TGGCAACGCC
    ATCCAGCATG TCCAGGCACT GCCGACAGAG AAGATTACCA AGAAGCCCAT CCCTGAGGAG
    CACCTTATTC TGAAGACTAC ATTTGAAGCT CTTATCCAGA GGTGCCTGCT GTCTGCTTCC
    GATCCGCAAA CCAAGAGGAA ACTAGATGAT GCAAACAAAC GCCTGGAGTT TCTATATGAC
    AAACTGAGGG AGCAGACACT CTCTCCCACC ATCGTGAGTG GTTTGCACAA CATGGTGAAG
    AGCATCGAAA CCCGCAACTA CCAGGAGGGG CTGAACATCC ACACGCACAT CGTCAGCACC
    AGCAACTTCA GCGAGACCTC CGCCTTCATG CCCGTGCTGA AGGTTGTCCT CACCCAGGCC
    AACAAGCTGG GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805485.1
    CDS462..4199
    Translation

    Target ORF information:

    RefSeq Version XM_014949999.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014949999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGACGT ATCAGGCCAC ACAGCAGTAC
    CCTTGTGGAA CGGGGGGACC AGCTCTCTAT CAGCCTCAGC AGCCTCTCGC TGTCCCTGCT
    TCAGCCTCCT ACCCAAACCC TGCCCCTAAC ACCACCCCTC CCTACCCCTC TGCCTCTGCC
    CACCCCATGC CCTCCCCGCT GTACCCCGGG CAGCCTCAGC CCTCCCCAAC AAGCCTGAAT
    CCCAGCTCTT CCTTCCCTCT TCCTCCTTCT GGCACGTCCT TTCAGCATGG CAGGCCAGGA
    CCTCCAGCAA CTTCTGTGGC TTATGCCTTG CCTACGGGAC CAACAGGGCC TCAGAATGGC
    TGGAACGACC CTCCCGCCCT GAACAGAGCT GCCAAGAAGA AGAAGGTCCC TGATAACTTC
    CTGCCCCCAG TGCCCATCAC CTCCCCCATC ATGAACCCGC TGGCGGATCC GCAGGCTCAG
    ATGCAGCAGC CCCCGGCTCC GGCAGCCCCC GTGGGTGCAC CCAGCTTCCA GCCTCCCCAC
    CTCCCCGTGG GGCAGGGGGG CTACCCACCT GCTCCCCACA CCCTGGGACC CTGCATCGTG
    CCGCCTGCCG TTGCCAAACC CAGCACAGAG GGGGCCCCGG GGGCCCCAAT TGGCAACGCC
    ATCCAGCATG TCCAGGCACT GCCGACAGAG AAGATTACCA AGAAGCCCAT CCCTGAGGAG
    CACCTTATTC TGAAGACTAC ATTTGAAGCT CTTATCCAGA GGTGCCTGCT GTCTGCTTCC
    GATCCGCAAA CCAAGAGGAA ACTAGATGAT GCAAACAAAC GCCTGGAGTT TCTATATGAC
    AAACTGAGGG AGCAGACACT CTCTCCCACC ATCGTGAGTG GTTTGCACAA CATGGTGAAG
    AGCATCGAAA CCCGCAACTA CCAGGAGGGG CTGAACATCC ACACGCACAT CGTCAGCACC
    AGCAACTTCA GCGAGACCTC CGCCTTCATG CCCGTGCTGA AGGTTGTCCT CACCCAGGCC
    AACAAGCTGG GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70930
    Clone ID Related Accession (Same CDS sequence) XM_014950000.1
    Accession Version XM_014950000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product protein transport protein Sec31A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGGAGAC
    AACCCACCTC TTCCAGGCTT CATCCTGCCG GGTGCCGTTA ACCCCAACGT GCCGTCGCAG
    CAAGCCGCGT CTTCCAATTG CGTCATGTAC CCACCAGCTG GGGCCCGACC AACCTACCTG
    CAGACGTATC AGGCCACACA GCAGTACCCT TGTGGAACGG GGGGACCAGC TCTCTATCAG
    CCTCAGCAGC CTCTCGCTGT CCCTGCTTCA GCCTCCTACC CAAACCCTGC CCCTAACACC
    ACCCCTCCCT ACCCCTCTGC CTCTGCCCAC CCCATGCCCT CCCCGCTGTA CCCCGGGCAG
    CCTCAGCCCT CCCCAACAAG CCTGAATCCC AGCTCTTCCT TCCCTCTTCC TCCTTCTGGC
    ACGTCCTTTC AGCATGGCAG GCCAGGACCT CCAGCAACTT CTGTGGCTTA TGCCTTGCCT
    ACGGGACCAA CAGGTACTCT GCCTGCAGCC AGCGACCTTC CTGCATCCCA GAGAACAGGG
    CCTCAGAATG GCTGGAACGA CCCTCCCGCC CTGAACAGAG CTGCCAAGAA GAAGAAGGTC
    CCTGATAACT TCCTGCCCCC AGTGCCCATC ACCTCCCCCA TCATGAACCC GCTGGCGGAT
    CCGCAGGCTC AGATGCAGCA GCCCCCGGCT CCGGCAGCCC CCGTGGGTGC ACCCAGCTTC
    CAGCCTCCCC ACCTCCCCGT GGGGCAGGGG GGCTACCCAC CTGCTCCCCA CACCCTGGGA
    CCCTGCATCG TGCCGCCTGC CGTTGCCAAA CCCAGCACAG AGGGGGCCCC GGGGGCCCCA
    ATTGGCAACG CCATCCAGCA TGTCCAGGCA CTGCCGACAG AGAAGATTAC CAAGAAGCCC
    ATCCCTGAGG AGCACCTTAT TCTGAAGACT ACATTTGAAG CTCTTATCCA GAGGTGCCTG
    CTGTCTGCTT CCGATCCGCA AACCAAGAGG AAACTAGATG ATGCAAACAA ACGCCTGGAG
    TTTCTATATG ACAAACTGAG GGAGCAGACA CTCTCTCCCA CCATCGTGAG TGGTTTGCAC
    AACATGGTGA AGAGCATCGA AACCCGCAAC TACCAGGAGG GGCTGAACAT CCACACGCAC
    ATCGTCAGCA CCAGCAACTT CAGCGAGACC TCCGCCTTCA TGCCCGTGCT GAAGGTTGTC
    CTCACCCAGG CCAACAAGCT GGGTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805486.1
    CDS462..4151
    Translation

    Target ORF information:

    RefSeq Version XM_014950000.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014950000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGGAGAC
    AACCCACCTC TTCCAGGCTT CATCCTGCCG GGTGCCGTTA ACCCCAACGT GCCGTCGCAG
    CAAGCCGCGT CTTCCAATTG CGTCATGTAC CCACCAGCTG GGGCCCGACC AACCTACCTG
    CAGACGTATC AGGCCACACA GCAGTACCCT TGTGGAACGG GGGGACCAGC TCTCTATCAG
    CCTCAGCAGC CTCTCGCTGT CCCTGCTTCA GCCTCCTACC CAAACCCTGC CCCTAACACC
    ACCCCTCCCT ACCCCTCTGC CTCTGCCCAC CCCATGCCCT CCCCGCTGTA CCCCGGGCAG
    CCTCAGCCCT CCCCAACAAG CCTGAATCCC AGCTCTTCCT TCCCTCTTCC TCCTTCTGGC
    ACGTCCTTTC AGCATGGCAG GCCAGGACCT CCAGCAACTT CTGTGGCTTA TGCCTTGCCT
    ACGGGACCAA CAGGTACTCT GCCTGCAGCC AGCGACCTTC CTGCATCCCA GAGAACAGGG
    CCTCAGAATG GCTGGAACGA CCCTCCCGCC CTGAACAGAG CTGCCAAGAA GAAGAAGGTC
    CCTGATAACT TCCTGCCCCC AGTGCCCATC ACCTCCCCCA TCATGAACCC GCTGGCGGAT
    CCGCAGGCTC AGATGCAGCA GCCCCCGGCT CCGGCAGCCC CCGTGGGTGC ACCCAGCTTC
    CAGCCTCCCC ACCTCCCCGT GGGGCAGGGG GGCTACCCAC CTGCTCCCCA CACCCTGGGA
    CCCTGCATCG TGCCGCCTGC CGTTGCCAAA CCCAGCACAG AGGGGGCCCC GGGGGCCCCA
    ATTGGCAACG CCATCCAGCA TGTCCAGGCA CTGCCGACAG AGAAGATTAC CAAGAAGCCC
    ATCCCTGAGG AGCACCTTAT TCTGAAGACT ACATTTGAAG CTCTTATCCA GAGGTGCCTG
    CTGTCTGCTT CCGATCCGCA AACCAAGAGG AAACTAGATG ATGCAAACAA ACGCCTGGAG
    TTTCTATATG ACAAACTGAG GGAGCAGACA CTCTCTCCCA CCATCGTGAG TGGTTTGCAC
    AACATGGTGA AGAGCATCGA AACCCGCAAC TACCAGGAGG GGCTGAACAT CCACACGCAC
    ATCGTCAGCA CCAGCAACTT CAGCGAGACC TCCGCCTTCA TGCCCGTGCT GAAGGTTGTC
    CTCACCCAGG CCAACAAGCT GGGTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa70931
    Clone ID Related Accession (Same CDS sequence) XM_014950001.1
    Accession Version XM_014950001.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product protein transport protein Sec31A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGAGGC CTCAGAATGG CTGGAACGAC
    CCTCCCGCCC TGAACAGAGC TGCCAAGAAG AAGAAGGTCC CTGATAACTT CCTGCCCCCA
    GTGCCCATCA CCTCCCCCAT CATGAACCCG CTGGCGGATC CGCAGGCTCA GATGCAGCAG
    CCCCCGGCTC CGGCAGCCCC CGTGGGTGCA CCCAGCTTCC AGCCTCCCCA CCTCCCCGTG
    GGGCAGGGGG GCTACCCACC TGCTCCCCAC ACCCTGGGAC CCTGCATCGT GCCGCCTGCC
    GTTGCCAAAC CCAGCACAGA GGGGGCCCCG GGGGCCCCAA TTGGCAACGC CATCCAGCAT
    GTCCAGGCAC TGCCGACAGA GAAGATTACC AAGAAGCCCA TCCCTGAGGA GCACCTTATT
    CTGAAGACTA CATTTGAAGC TCTTATCCAG AGGTGCCTGC TGTCTGCTTC CGATCCGCAA
    ACCAAGAGGA AACTAGATGA TGCAAACAAA CGCCTGGAGT TTCTATATGA CAAACTGAGG
    GAGCAGACAC TCTCTCCCAC CATCGTGAGT GGTTTGCACA ACATGGTGAA GAGCATCGAA
    ACCCGCAACT ACCAGGAGGG GCTGAACATC CACACGCACA TCGTCAGCAC CAGCAACTTC
    AGCGAGACCT CCGCCTTCAT GCCCGTGCTG AAGGTTGTCC TCACCCAGGC CAACAAGCTG
    GGTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805487.1
    CDS462..3890
    Translation

    Target ORF information:

    RefSeq Version XM_014950001.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax SEC31 homolog A, COPII coat complex component (SEC31A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014950001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAGAAATCGA TCGTACTGCC ATGCAGGCAT GGAGCCCTGC CCAGCAGCAC 
    CCCATTTATC TGGCCACAGG TACATCAGCT CAGCAGCTGG ATGCAACCTT CAGTACGAGT
    GCTTCTCTAG AAATATTCGA GTTGGACTTA TCAGACCCTT CGCTGGATAT GAAGTCCTGC
    GCCACCTTTT CCTCTTCTCA CAGGTACCAC AAGCTGATCT GGGGGCCGCA CAGCATGACC
    TCGGGTGAGA GGGTCTCTGG AGTCCTGATT GCCGGGGGTG AGAATGGCAA TGTCATTCTG
    TATGATCCAG CCAAAATCAT AGCTGGAGAT GCTGACGTCA TCATTGCCCA GAAAGACAAG
    CACACAGGAC CAGTAAGAGC GCTCGACGTC AACATGTTCC AGACCAATCT GGTGGCCTCT
    GGTGCTAACG AATCTGAAAT CTATATCTGG GACTTGAACA ATTTTGCCAC GCCAATGACA
    CCAGGAGTGA AAACACAGCC TCTGGAGGAC ATCAGCTGCA TCGCGTGGAA CAGGCAAGTC
    CAGCACATCC TGGCCTCTGC CAGCCCCAGC GGCCGAGCCA CCGTGTGGGA CCTCAGGAAG
    AACGAACCCA TCATCAAAGT CAGCGACCAC AACAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCTG ACGTTGCCAC CCAGATGGTG TTGGCCTCGG AGGATGACAG GTTGCCCGTG
    ATCCAGATGT GGGACCTGAG ATTTGCCTCC TCGCCACTTC GTGTTCTGGA AAGCCACACG
    AGGGGTATAT TGGCCATTGC ATGGAGTATG GCGGATTCGG AGCTGCTGCT TAGCTGCGGG
    AAGGATGCTA AAATTCTCTG CTCCAACCCT AACACGGGCG AGGTGTTGTA CGAGATGCCC
    ATGAACACGC AGTGGTGCTT TGATATCCAG TGGTGTCCCA GGAACCCCGC TGTCCTCTCT
    GCGGCCTCCT TTGACGGGCG GATCAGCGTT TACTCCATCA TGGGAGGCAG CGTGGATGGC
    TTGAGGCAGA AGCAAGTTGA CCAGCTTTCA TCATCTTTTG GGAACCTGGA TCCATTTGGA
    ACAGGACAGC CCCTCCCACC CCTGCAGCTT CCCCAGCAGA CTGCCCCCCA GGGTGTCGTT
    TTGCCCCTAA AGAAACCACC CAAATGGATC CGGCGACCTG TGGGAGCATC GTTCTCGTTT
    GGAGGCAAGC TGGTGACGTT TGAGAATGCC AAGGCTCAGC AGCAGCCAGG CATCGAGCAG
    CAGCAGCAGC AGCACCACGT CTACGTAAGC CAGGTTGTTA CGGAGAAAGA GTTCCTTGCC
    CGTTCAAACC AACTGCAGGA GGCTGTGCAG TCTGAGGGCT TCATCAGCTA CTGCCAGCAG
    AAAATCAACA TGGCCCAGGC TGACTTCGAG AAGAACGTGT GGGCCTTCTT AAAGGTGAAC
    TTTGAAGAAG ATTCACGGGT TAAATACCTT GAGCTCTTGG GATACAAGAA AGAAGATCTA
    AGGAAGAAGA TTGCATCCAC TCTGGATAAA GAGGGTCCTG CAGATGGTGA CGTGGGCGAG
    GTTCCTGCAG AATCTGATGA ATCTCTACCA AATGAGGGGG ATGAAGGCCA GGCTACAGAG
    GAGCAGCTCT TGAGAGAGAG GGTGAAGGAC CATAAACAAA AACCTGAAGA TTTGGGATCT
    GCAAAGAAGA CATTTAACAT CTCTGTTAGT GGAGATTTGG ACGGGTTGAT CACCCAGGCC
    TTGCTGACGG GTAACTTTGA GAGCGCGGTG GATCTCTGCT TGCACGATAA CCGGATGGCC
    GACGCCATCA TCCTGGCCAT CGCGGGCGGA CAGGAGCTGC TGTCTAGGAC ACAGGAAAAG
    TACTTTGCTA AGACACAGAG CAAAATTACC AGGCTCATCA CTGCAGTGGT AACAAAGAAC
    TGGAAGGAGA TTGTGCAGTC TTGTGACCTG CAGAACTGGA GAGAGGCTCT GGCTGCTGTG
    CTCACTTACG CCAGGCCGGA TGAATTTGCA GCCCTCTGCG ATCTCCTGGG TAACAGACTG
    GAGAGCGAGG GGGACAGCCT TCTGCAGACA CAGGCTTGTC TCTGTTACAT TTGTGCTGGC
    AATGTGGAAA AACTCGTTGC TTGTTGGACC AAAGCTCAGG ATGGAAACAA CCCCTTGTCC
    CTCCAGGATT TAATAGAGAA AGTGGTAATC CTGCGCAAAG CGGTGCAGCT CACCCAGGCT
    GTGGACCCCA ATGCTGTGGG AGCCCTTTTG GCTGAGAAGA TGAGCCAGTA TGCCAACCTC
    CTGGCTGCCC AGGGCAGTAT TGCTGCAGCT TTGACCTTCC TCCCTGCAAA CACCAACCAG
    CCCAACATCA TGCAGTTACG TGACAGGCTT TGCAGAGCCC AAGGGGAGCT CCCGGTGGGA
    CAGGAGACCT TCAAGGCACC CCACGAGAGA CAGCCCGTGC CCAAAGGACG AGCTGGCCCT
    GGGGCTGGAC AGTTGCAAGG GCCCCAGGCT CCAACCCAGC AGTATTACCA ACAGGTTAGA
    ATTGCCCCTA CTGTCACTAC CTGGAGTAAC AAAACTCCTA CTGCCCTTCC CAGCCATCTT
    CCTGCAGGCT GTCCCTCTGA CACACAGGGA GACAACCCAC CTCTTCCAGG CTTCATCCTG
    CCGGGTGCCG TTAACCCCAA CGTGCCGTCG CAGCAAGCCG CGTCTTCCAA TTGCGTCATG
    TACCCACCAG CTGGGGCCCG ACCAACCTAC CTGCAGAGGC CTCAGAATGG CTGGAACGAC
    CCTCCCGCCC TGAACAGAGC TGCCAAGAAG AAGAAGGTCC CTGATAACTT CCTGCCCCCA
    GTGCCCATCA CCTCCCCCAT CATGAACCCG CTGGCGGATC CGCAGGCTCA GATGCAGCAG
    CCCCCGGCTC CGGCAGCCCC CGTGGGTGCA CCCAGCTTCC AGCCTCCCCA CCTCCCCGTG
    GGGCAGGGGG GCTACCCACC TGCTCCCCAC ACCCTGGGAC CCTGCATCGT GCCGCCTGCC
    GTTGCCAAAC CCAGCACAGA GGGGGCCCCG GGGGCCCCAA TTGGCAACGC CATCCAGCAT
    GTCCAGGCAC TGCCGACAGA GAAGATTACC AAGAAGCCCA TCCCTGAGGA GCACCTTATT
    CTGAAGACTA CATTTGAAGC TCTTATCCAG AGGTGCCTGC TGTCTGCTTC CGATCCGCAA
    ACCAAGAGGA AACTAGATGA TGCAAACAAA CGCCTGGAGT TTCTATATGA CAAACTGAGG
    GAGCAGACAC TCTCTCCCAC CATCGTGAGT GGTTTGCACA ACATGGTGAA GAGCATCGAA
    ACCCGCAACT ACCAGGAGGG GCTGAACATC CACACGCACA TCGTCAGCAC CAGCAACTTC
    AGCGAGACCT CCGCCTTCAT GCCCGTGCTG AAGGTTGTCC TCACCCAGGC CAACAAGCTG
    GGTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

feedback

Do you like the current new website?

Hate

Dislike

Neutral

Like

Love

*