HARBI1 cDNA ORF clone, Calidris pugnax(ruff)

  • Gene

  • Clones

  • gRNAs

The following HARBI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HARBI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa71133 XM_014950286.1
Latest version!
Calidris pugnax harbinger transposase derived 1 (HARBI1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OCa71134 XM_014950288.1
Latest version!
Calidris pugnax harbinger transposase derived 1 (HARBI1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OCa71134 XM_014950289.1
Latest version!
Calidris pugnax harbinger transposase derived 1 (HARBI1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OCa71134 XM_014950287.1
Latest version!
Calidris pugnax harbinger transposase derived 1 (HARBI1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa71133
    Clone ID Related Accession (Same CDS sequence) XM_014950286.1
    Accession Version XM_014950286.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product putative nuclease HARBI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACTTATT TCTTGCCACA AGGAGGATTC TACCAGGTGG AGGCTGCGGG GAAGCCCCAG 
    GGGAGCCCCC GGCTGTTGGC CATGGCCGTA CCTATTGCCG TTCTTGACTG CGACCTCCTG
    CTCTACGGCC GCGGACACAG GACTTTGGAT CGCTTCAAGC TGGAGGATGT CACGGACGAG
    TATCTAGTGT CCACCTACGG CTTTCCCCGA CAATTCATTT ACTACCTGGT GGATCTTCTA
    GGAGCCAGTC TCTCTCGCCC TACACAGCGG TCCAGGGCCA TCAGTCCGGA GACGCAGATC
    CTTGCTGCAC TGGGTTTCTA CACCTCCGGC TCCTTCCAGA CTCGCATGGG GGATGCCATT
    GGCATTAGTC AAGCCTCCAT GAGCCGCTGC GTGGCCAACG TGACCGAGGC GTTGGTGGAA
    AGAGCCTCGC AGTTTATTCG CTTTCCTGAG GATGAAGCTA TGGTTCAGAG CCTGAAGGAT
    GACTTTTATG GGCTGGCAGG CATGCCGGGA GTGCTGGGGG TGGTCGACTG CACCCACGTG
    GCAATCAAAG CGCCAAATGC CGAGGACCTG TCCTACGTGA ACCGAAAGGG TCTCCATTCT
    CTGAACTGCC TGATGGTGTG TGATGCCAGA GGAGTCCTCC TGAGTGCAGA AACGCACTGG
    CCGGGCAGCC TGCCCGACTG CACAGTTCTG CAGCAGGCTG CCCTTACAAG CCAGTTTGAA
    AATGAGCTAC ATAAAGATGG CTGGCTACTT GGTGACAGCT CCTTCTTCCT CCGCACGTGG
    TTGATGACCC CTCTGCATAT CCCGGAGACA CCCGCAGAGT ACCGTTACAA CATGGCCCAT
    TCTGCCACTC ACAATGTCAT CGAGCGGACG TTCAGAACCA TCCGGTCGCG TTTCCGCTGC
    CTGGATGGCT CCAAAGGCAC CCTGCAGTAT TCTCCAGAGA AATCCAGCCA CATCATTCTG
    GCCTGCTGCG TGCTTCACAA CATCTCCCTG GAACACGGGC TGGATGTGTG GTCTTCGCCG
    GCCACAGGAC ACATGGAACA ACCAGAAGAA GAGTACGAGC AAATGGAATC AATGGACTCG
    GAAGCCTGTC GTATTCGTCA GGAGCTTTTA CTTACACATT TTAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805772.1
    CDS584..1711
    Translation

    Target ORF information:

    RefSeq Version XM_014950286.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax harbinger transposase derived 1 (HARBI1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014950286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACTTATT TCTTGCCACA AGGAGGATTC TACCAGGTGG AGGCTGCGGG GAAGCCCCAG 
    GGGAGCCCCC GGCTGTTGGC CATGGCCGTA CCTATTGCCG TTCTTGACTG CGACCTCCTG
    CTCTACGGCC GCGGACACAG GACTTTGGAT CGCTTCAAGC TGGAGGATGT CACGGACGAG
    TATCTAGTGT CCACCTACGG CTTTCCCCGA CAATTCATTT ACTACCTGGT GGATCTTCTA
    GGAGCCAGTC TCTCTCGCCC TACACAGCGG TCCAGGGCCA TCAGTCCGGA GACGCAGATC
    CTTGCTGCAC TGGGTTTCTA CACCTCCGGC TCCTTCCAGA CTCGCATGGG GGATGCCATT
    GGCATTAGTC AAGCCTCCAT GAGCCGCTGC GTGGCCAACG TGACCGAGGC GTTGGTGGAA
    AGAGCCTCGC AGTTTATTCG CTTTCCTGAG GATGAAGCTA TGGTTCAGAG CCTGAAGGAT
    GACTTTTATG GGCTGGCAGG CATGCCGGGA GTGCTGGGGG TGGTCGACTG CACCCACGTG
    GCAATCAAAG CGCCAAATGC CGAGGACCTG TCCTACGTGA ACCGAAAGGG TCTCCATTCT
    CTGAACTGCC TGATGGTGTG TGATGCCAGA GGAGTCCTCC TGAGTGCAGA AACGCACTGG
    CCGGGCAGCC TGCCCGACTG CACAGTTCTG CAGCAGGCTG CCCTTACAAG CCAGTTTGAA
    AATGAGCTAC ATAAAGATGG CTGGCTACTT GGTGACAGCT CCTTCTTCCT CCGCACGTGG
    TTGATGACCC CTCTGCATAT CCCGGAGACA CCCGCAGAGT ACCGTTACAA CATGGCCCAT
    TCTGCCACTC ACAATGTCAT CGAGCGGACG TTCAGAACCA TCCGGTCGCG TTTCCGCTGC
    CTGGATGGCT CCAAAGGCAC CCTGCAGTAT TCTCCAGAGA AATCCAGCCA CATCATTCTG
    GCCTGCTGCG TGCTTCACAA CATCTCCCTG GAACACGGGC TGGATGTGTG GTCTTCGCCG
    GCCACAGGAC ACATGGAACA ACCAGAAGAA GAGTACGAGC AAATGGAATC AATGGACTCG
    GAAGCCTGTC GTATTCGTCA GGAGCTTTTA CTTACACATT TTAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa71134
    Clone ID Related Accession (Same CDS sequence) XM_014950288.1 , XM_014950287.1 , XM_014950289.1
    Accession Version XM_014950288.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product putative nuclease HARBI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCGTAC CTATTGCCGT TCTTGACTGC GACCTCCTGC TCTACGGCCG CGGACACAGG 
    ACTTTGGATC GCTTCAAGCT GGAGGATGTC ACGGACGAGT ATCTAGTGTC CACCTACGGC
    TTTCCCCGAC AATTCATTTA CTACCTGGTG GATCTTCTAG GAGCCAGTCT CTCTCGCCCT
    ACACAGCGGT CCAGGGCCAT CAGTCCGGAG ACGCAGATCC TTGCTGCACT GGGTTTCTAC
    ACCTCCGGCT CCTTCCAGAC TCGCATGGGG GATGCCATTG GCATTAGTCA AGCCTCCATG
    AGCCGCTGCG TGGCCAACGT GACCGAGGCG TTGGTGGAAA GAGCCTCGCA GTTTATTCGC
    TTTCCTGAGG ATGAAGCTAT GGTTCAGAGC CTGAAGGATG ACTTTTATGG GCTGGCAGGC
    ATGCCGGGAG TGCTGGGGGT GGTCGACTGC ACCCACGTGG CAATCAAAGC GCCAAATGCC
    GAGGACCTGT CCTACGTGAA CCGAAAGGGT CTCCATTCTC TGAACTGCCT GATGGTGTGT
    GATGCCAGAG GAGTCCTCCT GAGTGCAGAA ACGCACTGGC CGGGCAGCCT GCCCGACTGC
    ACAGTTCTGC AGCAGGCTGC CCTTACAAGC CAGTTTGAAA ATGAGCTACA TAAAGATGGC
    TGGCTACTTG GTGACAGCTC CTTCTTCCTC CGCACGTGGT TGATGACCCC TCTGCATATC
    CCGGAGACAC CCGCAGAGTA CCGTTACAAC ATGGCCCATT CTGCCACTCA CAATGTCATC
    GAGCGGACGT TCAGAACCAT CCGGTCGCGT TTCCGCTGCC TGGATGGCTC CAAAGGCACC
    CTGCAGTATT CTCCAGAGAA ATCCAGCCAC ATCATTCTGG CCTGCTGCGT GCTTCACAAC
    ATCTCCCTGG AACACGGGCT GGATGTGTGG TCTTCGCCGG CCACAGGACA CATGGAACAA
    CCAGAAGAAG AGTACGAGCA AATGGAATCA ATGGACTCGG AAGCCTGTCG TATTCGTCAG
    GAGCTTTTAC TTACACATTT TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805774.1
    CDS91..1137
    Translation

    Target ORF information:

    RefSeq Version XM_014950288.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax harbinger transposase derived 1 (HARBI1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014950288.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCGTAC CTATTGCCGT TCTTGACTGC GACCTCCTGC TCTACGGCCG CGGACACAGG 
    ACTTTGGATC GCTTCAAGCT GGAGGATGTC ACGGACGAGT ATCTAGTGTC CACCTACGGC
    TTTCCCCGAC AATTCATTTA CTACCTGGTG GATCTTCTAG GAGCCAGTCT CTCTCGCCCT
    ACACAGCGGT CCAGGGCCAT CAGTCCGGAG ACGCAGATCC TTGCTGCACT GGGTTTCTAC
    ACCTCCGGCT CCTTCCAGAC TCGCATGGGG GATGCCATTG GCATTAGTCA AGCCTCCATG
    AGCCGCTGCG TGGCCAACGT GACCGAGGCG TTGGTGGAAA GAGCCTCGCA GTTTATTCGC
    TTTCCTGAGG ATGAAGCTAT GGTTCAGAGC CTGAAGGATG ACTTTTATGG GCTGGCAGGC
    ATGCCGGGAG TGCTGGGGGT GGTCGACTGC ACCCACGTGG CAATCAAAGC GCCAAATGCC
    GAGGACCTGT CCTACGTGAA CCGAAAGGGT CTCCATTCTC TGAACTGCCT GATGGTGTGT
    GATGCCAGAG GAGTCCTCCT GAGTGCAGAA ACGCACTGGC CGGGCAGCCT GCCCGACTGC
    ACAGTTCTGC AGCAGGCTGC CCTTACAAGC CAGTTTGAAA ATGAGCTACA TAAAGATGGC
    TGGCTACTTG GTGACAGCTC CTTCTTCCTC CGCACGTGGT TGATGACCCC TCTGCATATC
    CCGGAGACAC CCGCAGAGTA CCGTTACAAC ATGGCCCATT CTGCCACTCA CAATGTCATC
    GAGCGGACGT TCAGAACCAT CCGGTCGCGT TTCCGCTGCC TGGATGGCTC CAAAGGCACC
    CTGCAGTATT CTCCAGAGAA ATCCAGCCAC ATCATTCTGG CCTGCTGCGT GCTTCACAAC
    ATCTCCCTGG AACACGGGCT GGATGTGTGG TCTTCGCCGG CCACAGGACA CATGGAACAA
    CCAGAAGAAG AGTACGAGCA AATGGAATCA ATGGACTCGG AAGCCTGTCG TATTCGTCAG
    GAGCTTTTAC TTACACATTT TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa71134
    Clone ID Related Accession (Same CDS sequence) XM_014950288.1 , XM_014950287.1 , XM_014950289.1
    Accession Version XM_014950287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product putative nuclease HARBI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCGTAC CTATTGCCGT TCTTGACTGC GACCTCCTGC TCTACGGCCG CGGACACAGG 
    ACTTTGGATC GCTTCAAGCT GGAGGATGTC ACGGACGAGT ATCTAGTGTC CACCTACGGC
    TTTCCCCGAC AATTCATTTA CTACCTGGTG GATCTTCTAG GAGCCAGTCT CTCTCGCCCT
    ACACAGCGGT CCAGGGCCAT CAGTCCGGAG ACGCAGATCC TTGCTGCACT GGGTTTCTAC
    ACCTCCGGCT CCTTCCAGAC TCGCATGGGG GATGCCATTG GCATTAGTCA AGCCTCCATG
    AGCCGCTGCG TGGCCAACGT GACCGAGGCG TTGGTGGAAA GAGCCTCGCA GTTTATTCGC
    TTTCCTGAGG ATGAAGCTAT GGTTCAGAGC CTGAAGGATG ACTTTTATGG GCTGGCAGGC
    ATGCCGGGAG TGCTGGGGGT GGTCGACTGC ACCCACGTGG CAATCAAAGC GCCAAATGCC
    GAGGACCTGT CCTACGTGAA CCGAAAGGGT CTCCATTCTC TGAACTGCCT GATGGTGTGT
    GATGCCAGAG GAGTCCTCCT GAGTGCAGAA ACGCACTGGC CGGGCAGCCT GCCCGACTGC
    ACAGTTCTGC AGCAGGCTGC CCTTACAAGC CAGTTTGAAA ATGAGCTACA TAAAGATGGC
    TGGCTACTTG GTGACAGCTC CTTCTTCCTC CGCACGTGGT TGATGACCCC TCTGCATATC
    CCGGAGACAC CCGCAGAGTA CCGTTACAAC ATGGCCCATT CTGCCACTCA CAATGTCATC
    GAGCGGACGT TCAGAACCAT CCGGTCGCGT TTCCGCTGCC TGGATGGCTC CAAAGGCACC
    CTGCAGTATT CTCCAGAGAA ATCCAGCCAC ATCATTCTGG CCTGCTGCGT GCTTCACAAC
    ATCTCCCTGG AACACGGGCT GGATGTGTGG TCTTCGCCGG CCACAGGACA CATGGAACAA
    CCAGAAGAAG AGTACGAGCA AATGGAATCA ATGGACTCGG AAGCCTGTCG TATTCGTCAG
    GAGCTTTTAC TTACACATTT TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805773.1
    CDS162..1208
    Translation

    Target ORF information:

    RefSeq Version XM_014950287.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax harbinger transposase derived 1 (HARBI1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014950287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCGTAC CTATTGCCGT TCTTGACTGC GACCTCCTGC TCTACGGCCG CGGACACAGG 
    ACTTTGGATC GCTTCAAGCT GGAGGATGTC ACGGACGAGT ATCTAGTGTC CACCTACGGC
    TTTCCCCGAC AATTCATTTA CTACCTGGTG GATCTTCTAG GAGCCAGTCT CTCTCGCCCT
    ACACAGCGGT CCAGGGCCAT CAGTCCGGAG ACGCAGATCC TTGCTGCACT GGGTTTCTAC
    ACCTCCGGCT CCTTCCAGAC TCGCATGGGG GATGCCATTG GCATTAGTCA AGCCTCCATG
    AGCCGCTGCG TGGCCAACGT GACCGAGGCG TTGGTGGAAA GAGCCTCGCA GTTTATTCGC
    TTTCCTGAGG ATGAAGCTAT GGTTCAGAGC CTGAAGGATG ACTTTTATGG GCTGGCAGGC
    ATGCCGGGAG TGCTGGGGGT GGTCGACTGC ACCCACGTGG CAATCAAAGC GCCAAATGCC
    GAGGACCTGT CCTACGTGAA CCGAAAGGGT CTCCATTCTC TGAACTGCCT GATGGTGTGT
    GATGCCAGAG GAGTCCTCCT GAGTGCAGAA ACGCACTGGC CGGGCAGCCT GCCCGACTGC
    ACAGTTCTGC AGCAGGCTGC CCTTACAAGC CAGTTTGAAA ATGAGCTACA TAAAGATGGC
    TGGCTACTTG GTGACAGCTC CTTCTTCCTC CGCACGTGGT TGATGACCCC TCTGCATATC
    CCGGAGACAC CCGCAGAGTA CCGTTACAAC ATGGCCCATT CTGCCACTCA CAATGTCATC
    GAGCGGACGT TCAGAACCAT CCGGTCGCGT TTCCGCTGCC TGGATGGCTC CAAAGGCACC
    CTGCAGTATT CTCCAGAGAA ATCCAGCCAC ATCATTCTGG CCTGCTGCGT GCTTCACAAC
    ATCTCCCTGG AACACGGGCT GGATGTGTGG TCTTCGCCGG CCACAGGACA CATGGAACAA
    CCAGAAGAAG AGTACGAGCA AATGGAATCA ATGGACTCGG AAGCCTGTCG TATTCGTCAG
    GAGCTTTTAC TTACACATTT TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa71134
    Clone ID Related Accession (Same CDS sequence) XM_014950288.1 , XM_014950287.1 , XM_014950289.1
    Accession Version XM_014950289.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product putative nuclease HARBI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCGTAC CTATTGCCGT TCTTGACTGC GACCTCCTGC TCTACGGCCG CGGACACAGG 
    ACTTTGGATC GCTTCAAGCT GGAGGATGTC ACGGACGAGT ATCTAGTGTC CACCTACGGC
    TTTCCCCGAC AATTCATTTA CTACCTGGTG GATCTTCTAG GAGCCAGTCT CTCTCGCCCT
    ACACAGCGGT CCAGGGCCAT CAGTCCGGAG ACGCAGATCC TTGCTGCACT GGGTTTCTAC
    ACCTCCGGCT CCTTCCAGAC TCGCATGGGG GATGCCATTG GCATTAGTCA AGCCTCCATG
    AGCCGCTGCG TGGCCAACGT GACCGAGGCG TTGGTGGAAA GAGCCTCGCA GTTTATTCGC
    TTTCCTGAGG ATGAAGCTAT GGTTCAGAGC CTGAAGGATG ACTTTTATGG GCTGGCAGGC
    ATGCCGGGAG TGCTGGGGGT GGTCGACTGC ACCCACGTGG CAATCAAAGC GCCAAATGCC
    GAGGACCTGT CCTACGTGAA CCGAAAGGGT CTCCATTCTC TGAACTGCCT GATGGTGTGT
    GATGCCAGAG GAGTCCTCCT GAGTGCAGAA ACGCACTGGC CGGGCAGCCT GCCCGACTGC
    ACAGTTCTGC AGCAGGCTGC CCTTACAAGC CAGTTTGAAA ATGAGCTACA TAAAGATGGC
    TGGCTACTTG GTGACAGCTC CTTCTTCCTC CGCACGTGGT TGATGACCCC TCTGCATATC
    CCGGAGACAC CCGCAGAGTA CCGTTACAAC ATGGCCCATT CTGCCACTCA CAATGTCATC
    GAGCGGACGT TCAGAACCAT CCGGTCGCGT TTCCGCTGCC TGGATGGCTC CAAAGGCACC
    CTGCAGTATT CTCCAGAGAA ATCCAGCCAC ATCATTCTGG CCTGCTGCGT GCTTCACAAC
    ATCTCCCTGG AACACGGGCT GGATGTGTGG TCTTCGCCGG CCACAGGACA CATGGAACAA
    CCAGAAGAAG AGTACGAGCA AATGGAATCA ATGGACTCGG AAGCCTGTCG TATTCGTCAG
    GAGCTTTTAC TTACACATTT TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805775.1
    CDS181..1227
    Translation

    Target ORF information:

    RefSeq Version XM_014950289.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax harbinger transposase derived 1 (HARBI1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014950289.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCGTAC CTATTGCCGT TCTTGACTGC GACCTCCTGC TCTACGGCCG CGGACACAGG 
    ACTTTGGATC GCTTCAAGCT GGAGGATGTC ACGGACGAGT ATCTAGTGTC CACCTACGGC
    TTTCCCCGAC AATTCATTTA CTACCTGGTG GATCTTCTAG GAGCCAGTCT CTCTCGCCCT
    ACACAGCGGT CCAGGGCCAT CAGTCCGGAG ACGCAGATCC TTGCTGCACT GGGTTTCTAC
    ACCTCCGGCT CCTTCCAGAC TCGCATGGGG GATGCCATTG GCATTAGTCA AGCCTCCATG
    AGCCGCTGCG TGGCCAACGT GACCGAGGCG TTGGTGGAAA GAGCCTCGCA GTTTATTCGC
    TTTCCTGAGG ATGAAGCTAT GGTTCAGAGC CTGAAGGATG ACTTTTATGG GCTGGCAGGC
    ATGCCGGGAG TGCTGGGGGT GGTCGACTGC ACCCACGTGG CAATCAAAGC GCCAAATGCC
    GAGGACCTGT CCTACGTGAA CCGAAAGGGT CTCCATTCTC TGAACTGCCT GATGGTGTGT
    GATGCCAGAG GAGTCCTCCT GAGTGCAGAA ACGCACTGGC CGGGCAGCCT GCCCGACTGC
    ACAGTTCTGC AGCAGGCTGC CCTTACAAGC CAGTTTGAAA ATGAGCTACA TAAAGATGGC
    TGGCTACTTG GTGACAGCTC CTTCTTCCTC CGCACGTGGT TGATGACCCC TCTGCATATC
    CCGGAGACAC CCGCAGAGTA CCGTTACAAC ATGGCCCATT CTGCCACTCA CAATGTCATC
    GAGCGGACGT TCAGAACCAT CCGGTCGCGT TTCCGCTGCC TGGATGGCTC CAAAGGCACC
    CTGCAGTATT CTCCAGAGAA ATCCAGCCAC ATCATTCTGG CCTGCTGCGT GCTTCACAAC
    ATCTCCCTGG AACACGGGCT GGATGTGTGG TCTTCGCCGG CCACAGGACA CATGGAACAA
    CCAGAAGAAG AGTACGAGCA AATGGAATCA ATGGACTCGG AAGCCTGTCG TATTCGTCAG
    GAGCTTTTAC TTACACATTT TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.