MAPKBP1 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAPKBP1
Entrez Gene ID	106892809
Full Name	mitogen-activated protein kinase binding protein 1
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014950327.1	XP_014805813.1	mitogen-activated protein kinase-binding protein 1 isoform X2
XM_014950326.1	XP_014805812.1	mitogen-activated protein kinase-binding protein 1 isoform X1
XM_014950328.1	XP_014805814.1	mitogen-activated protein kinase-binding protein 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following MAPKBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAPKBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa71163	XM_014950326.1 Latest version!	Calidris pugnax mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa71164	XM_014950327.1 Latest version!	Calidris pugnax mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa71165	XM_014950328.1 Latest version!	Calidris pugnax mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAPKBP1 ( XM_014950326.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014805812.1 Calidris pugnax mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa71163
Clone ID Related Accession (Same CDS sequence)	XM_014950326.1
Accession Version	XM_014950326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG TCGAAGGGTC TACCATAACA AGTCGGATCA AAAATCTGCT TCGCTCTCCT 
TCCATTAAGC TGAGGAGAAG CAAGCCTGGG AACAAGCGAG AAGATATAGG CAGCAAGGTG 
ACTTTGGAGA AGGTGTTGGG GATAACAGTG TCAGGAGGCA GAGGATTAGC CTGCGACCCC 
AGAACTGGCC TCGTTGCTTA TCCAGCAGGG TGTGTTGTTG TGCTGTTCAA CCCTAGAAAA 
AACAAGCAAC ACCATATTCT AAACAGTTCC AGGAAAACAA TTACAGCACT TGCTTTCTCT 
CCAGATGGAA AATATTTGGT TACAGGAGAG AGTGGACACA TGCCAGCAGT CCGGGTATGG 
GATGTGGCTG AGCGAACCCA GGTGGCTGAG CTCCAGGAGC ACAAGTATGG CGTGGCCTGT 
GTGGCCTTCT CCCCCAGCTC CAAGTACATT GTTTCAGTGG GGTACCAGCA TGACATGATT 
GTCAACGTCT GGTCATGGAA GAAAAACATT GTGGTGGCAG CCAACAAAGT CTCGAGTAAA 
GTGACAGCCG TGTCATTCTC GGAGGACTGC AGTTACTTTG TCACTGCTGG AAATCGACAC 
ATCAAGTTCT GGTACCTGGA TGACAGCAAA ACCTCCAAGG TCAATGCCAC AGTCCCCTTG 
CTGGGCCGCT CAGGATTGCT TGGAGAACTG AGGAACAACT TCTTTGCGGA CGTGGCGTGT 
GGAAGGGGCA AAAAAGCTGA CAGCACCTTC TGTATCACGT CCTCAGGCCT GCTCTGTGAA 
TTCAATGAAA AAAGGCTGCT GGACAAGTGG GTGGAGCTGA GGAATACAGA CAGCTTCACA 
ACCACTGTGG CAAACTGCAT CTCTGTGAAT CATGACTACA TCTTCTGTGG CTGCGCTGAC 
GGCACCGTGA GGATTTTTAA CCCTCTGAAC CTGCACTTTG TCACCACGCT GCCCAAGCCA 
CACTTCCTGG GAACAGACAT CGCGAGTGTG ACCGATGCCA GTCGTCTTTT CTCTGGTATG 
GCTGATGCGA AGTATCCAGA CACGATTGCC TTGACTTTTG ACCCCACCAA CCAGTGGCTT 
TCCTGTGTAT ACAATGACCA CAGCCTGTAC GTGTGGGATG TCAAAGACCC GAAGAAAGTG 
GGCAAGGTGT ACTCCGCTTT GTACCACTCT TCCTGCGTGT GGAACATTGA GATGTATCCA 
GAGGTGAAGG ACAGCAATCA GTCGTGCCTC CCCCCTGGTT CCTTCATCAC GTGCTCCTCT 
GACAACACCA TTCGCCTGTG GAACACGGAG AGCTCCAACA TTCACGGCAC AGCCCTGCAC 
CGCAACATCC TCAGCAATGA CTTGATGAAA ATCATCTATG TAGATGACAA CACTCAGGTA 
CTTCTGGACA CTGATTACAA CTCGGCAGGA AGTGCAGAGA AAGCTGATGC ACAAGTGATG 
GATACGAAGG TGGGGATCCG CACCGTCTGT GTGAGTCCCA GTGGGGAGCA CCTGGCCTCA 
GGGGACAGGA TAGGCACTCT CAGGATATAT GAGTTGCAGT CTCTGAGGGA AATGCTGAAG 
GTGGAAGCCC ATGACTCTGA GATCCTGTGT CTGGAGTACT CCAAACCTGA TACAGGCTTA 
AAGCTCTTAG CCTCAGCCAG TCGGGATAGA TTGATTCATG TCTTGGATGC TGGAAAGGAC 
TACAGCCTGC AGCAGACCCT GGATGAACAT TCTTCTTCTA TCACTGCTGT GAAGTTTGCA 
GCCAATGATG GGAAAGTGAG AATGATAAGC TGTGGGGCAG ACAAGAGCAT CTATTTCCGC 
ACTGCGCAGA AGACAGGAGA AGGGGTCCAG TTTACCCGAA CACATCACAT TGTTAGGAAG 
ACTACTCTGT ATGACATGGA TGTGGACCCC AGCTGGAAAT ATGCAGCTAT TGGATGCCAG 
GATCGTAACA TCAGGGTTTT CAACATAAGC AGTGGGAAGC AGAAGAAGCT TTACAAAGGA 
TCCCAAGGTG AAGATGGTAC CCTCATCAAA GTGCAAACAG ATCCCTCTGG TCTTTATATT 
GCCACCAGTT GTTCGGACAA GAACCTCTCC ATCTTTGATT TCTATTCTGG CGAGTGTGTT 
GCAACCATGT ATGGACATTC AGAGATTGTA ACCGGGATGA AATTCAGTAA TGACTGCAAA 
CATCTGATCT CTGTTTCTGG TGACAGCTGT ATCTTCATCT GGCGTCTGAG CTCGGAGATG 
ACCATCAACA TGCGGCAGCG ACTGTCTGAC ATGAAGCAGA GAGGGAAGCA GGCAGAAAAA 
TCTCCTCCGC ACAAGACAGC TGGACTCATG AGGCACGAGT CCATCTCTGC CCTGTCCTCT 
GTGCCTGCGC TCTCATCAGA CAGTGACAAG GATGGTGAAG ACGAAGGGAA TGATGAAGAT 
GAGCTACGTG GGCTGCAGTC TTTCCATCTT CAGTCCAACT GCAGCACTGA GAGAGACTCA 
GATCCAGACC TTACTCTCTC AAGAAGCCTT TCCCATTGGG AAATGAGGAG GGCCAAAGAG 
ATAGCGGCGA TCCAGCGCTC AGAGGCACCA ATGAGCAAAA TGCCCCGGCA GCGCGGGCGC 
TGGTCGCAGC CCACCAGCAA CATAGAGATG ACGGTGAAAT CCATGCTTGA CTTGCGTCAG 
TTGGAGTCTT TCTCCATTTC CCGTTCTCCC AGTCGAGACT CACTGTCTCA AAACAGCAAC 
GGGGACGACA GAGAAGAGCA GGCAGATCTT CCTGCACACA TCAACAAAGT TGGCAGCTCA 
GTACCTCCCC AAGAGAACCG GTCTTGTGTG CGACCCCAAG TGATTCGCCT TGTGTCTTGT 
GAAGAGGGGA TTTTCTCTCA GGAACTGGAA CCTTCTGAGA GTGAGGAGTG TGTAATCTAC 
CCTGAACAAG ATGAAATCCA TCCCACAGAC TCTAGCAGTG AGTTCCAGGT AAAAGCACTG 
CCCCACGGGA AGCTCAGTAG AGGTTATCAC AGCAATGGAT GCCCAGACAA ACACAGTCCT 
GACAGTGCCT GCTCTGTAGA TTACAGCAGC AGTCGTCTGT CCAGCCCAGA CCATCCGAAT 
GAAGATTCTG AAAGCACTGA ACCCCTGAGT GTGGATGGTG TCTCAGACCT GGAGGGAGAG 
GAAGGAGAGG AGGACGTGGG TACTACCATG CCGGAAGGAG AAATCCCCCG GACACCCGAT 
CAGGAGAAGT TTCTTAAACA GCATTTTGGG TCCCTGGGCA GCACTGATGG AAAAGGTGGC 
TCATGTAGGA ATTTGGAAAG AACTGAAAAC CTCAGCATTT CCTCTCGCTT TCTTTCCCAG 
TCCTCGGCTC TCAGACAATT GTCACTCTCT TCATCAAATC TGATACTGGA TTCAAAGCCC 
ATTGAGCCGC CAACGCAGAC CAGTCGGCTT ACACCAGACC TGTTGAAAAA TGGCAGCGAC 
CATCCTGGTG ATGCATTGGA GAACGGCCAG CTCCTGGAGA GTGTAAATTC AAATCGGACT 
GTTTATGTCC AGAAAAAACG CAGATCAGCT TTGGAGGCCA GCAGACTTGG TATGGCTCCT 
GGACGGGTCA TCGCATCCTT TCCAGAAGGC CCTGTGAATG CAGTGATGAG AAAGGCACAG 
TCAGTTCACG ACCTCGTTCA TGAGGATAAG GGTCCTGGAG TGGTCTCCTC TGTCAAGCAG 
CGTCCTCAGA CTCTTTTGGT GGTTGAGAAG GATCCCAGAC CTAACAATTG CAGGGTGTTA 
TCAACCAGTA TGTTGAAGAT GGATGGATCT GGTGCTCTGC TGTCCAAAGA TGTCAGGGCT 
GCAAAATCAA AGTCCTACAT GAACCCCACA ACCAGCTTCC GGGCTAAGAT GTCAAGGAGC 
GTATCTGTAG GGGAAAATCT GTACCTCGGT TACTCCGCCG AAATGTTGAC TGATGGGAGG 
ACTAGCCCTC CAGTGGCAGA GAAGACACAG TTTAGTTCTA CAGCAGATGC TGAGAAGATT 
AAGCCACCAG CAAAAGAAAA TGTTCCAGTG AAGCCAGCAC TTCAACCAGT TGTAAACTGC 
TCATCTCCCA AGTCCTTCCA CAGCAAGTTG GCATCGTCAA ACCGAGCACA TCTGATTCTT 
GACATTCCTA AACCATTGCC TGATAGACCC ACACTGGCCT CCTTCTCACC CACTACCAAA 
CCAAAAACCC TACTTGAGCC ACAGTCTCCA CAGTCACCTG CTGGGGCCTG CAAAAAGAAA 
CCCTCTTTTC CTGAGGTCCG AGCCTCCAAG AGGGAGAATC AAACTGCTGG CTCTCCGGTT 
TCTGGTAAAG AGATCCCAAC AGGACTTGCT AAGGAGAGCC CAGTGGAAAG TCCGGTCTCA 
AGGACAGGTT TCCAGGATGA GCCAGGGGTC ACTAATCCAA AACTGAGGGA GTTGTCAGAG 
GGCCGGCACC TAAGGTCTCC TGAAGGTACA CTGCCCAGGT GCAGGGAGAG GATCACCGGC 
ATCGCCTGTG TATTAGACAA CCAACCTGGA CTTTGCCCAC TAGACCCCAT CAGGCCGAGG 
TCTCCTACAT CTATAACGGC CTCGGCACAG GTCTCAGAAT CGTGCATCAG CGTGGAGCAG 
TGTGAGCACG TGGTGTCCGA GCTCCAGGAC AGCATGCGCA AAGCCCTTCA TCTGTACCGC 
ATGGTGTTAA ATGATACGGA GTCTTCTACT GACAAAGATA AAATAGCGGG CCTCCTGACA 
GAAACATTCT CCTCAATGAA GAAAGAATTG GACTCTCTGA AGGATGAGGA GGAGCTTCCA 
AAGGTTAAGG AGGTACTAGC GAAGCCACAA GATACCACTA GCCCACTGAA TGAGGGATGT 
GGTGCCAACC TACCGAGTCC CAAGAGTTTG GGAGATGAGC AGACACTAGC TTTGCTGGAG 
CAGTACTCAG AGCTGTTGCT ACAAGCTGTG GAACGACGCA TGGACAAAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014805812.1
CDS	220..5136
Translation	MMSVEGSTITSRIKNLLRSPSIKLRRSKPGNKREDIGSKVTLEKVLGITVSGGRGLACDPRTGLVAYPAGCVVVLFNPRKNKQHHILNSSRKTITALAFSPDGKYLVTGESGHMPAVRVWDVAERTQVAELQEHKYGVACVAFSPSSKYIVSVGYQHDMIVNVWSWKKNIVVAANKVSSKVTAVSFSEDCSYFVTAGNRHIKFWYLDDSKTSKVNATVPLLGRSGLLGELRNNFFADVACGRGKKADSTFCITSSGLLCEFNEKRLLDKWVELRNTDSFTTTVANCISVNHDYIFCGCADGTVRIFNPLNLHFVTTLPKPHFLGTDIASVTDASRLFSGMADAKYPDTIALTFDPTNQWLSCVYNDHSLYVWDVKDPKKVGKVYSALYHSSCVWNIEMYPEVKDSNQSCLPPGSFITCSSDNTIRLWNTESSNIHGTALHRNILSNDLMKIIYVDDNTQVLLDTDYNSAGSAEKADAQVMDTKVGIRTVCVSPSGEHLASGDRIGTLRIYELQSLREMLKVEAHDSEILCLEYSKPDTGLKLLASASRDRLIHVLDAGKDYSLQQTLDEHSSSITAVKFAANDGKVRMISCGADKSIYFRTAQKTGEGVQFTRTHHIVRKTTLYDMDVDPSWKYAAIGCQDRNIRVFNISSGKQKKLYKGSQGEDGTLIKVQTDPSGLYIATSCSDKNLSIFDFYSGECVATMYGHSEIVTGMKFSNDCKHLISVSGDSCIFIWRLSSEMTINMRQRLSDMKQRGKQAEKSPPHKTAGLMRHESISALSSVPALSSDSDKDGEDEGNDEDELRGLQSFHLQSNCSTERDSDPDLTLSRSLSHWEMRRAKEIAAIQRSEAPMSKMPRQRGRWSQPTSNIEMTVKSMLDLRQLESFSISRSPSRDSLSQNSNGDDREEQADLPAHINKVGSSVPPQENRSCVRPQVIRLVSCEEGIFSQELEPSESEECVIYPEQDEIHPTDSSSEFQVKALPHGKLSRGYHSNGCPDKHSPDSACSVDYSSSRLSSPDHPNEDSESTEPLSVDGVSDLEGEEGEEDVGTTMPEGEIPRTPDQEKFLKQHFGSLGSTDGKGGSCRNLERTENLSISSRFLSQSSALRQLSLSSSNLILDSKPIEPPTQTSRLTPDLLKNGSDHPGDALENGQLLESVNSNRTVYVQKKRRSALEASRLGMAPGRVIASFPEGPVNAVMRKAQSVHDLVHEDKGPGVVSSVKQRPQTLLVVEKDPRPNNCRVLSTSMLKMDGSGALLSKDVRAAKSKSYMNPTTSFRAKMSRSVSVGENLYLGYSAEMLTDGRTSPPVAEKTQFSSTADAEKIKPPAKENVPVKPALQPVVNCSSPKSFHSKLASSNRAHLILDIPKPLPDRPTLASFSPTTKPKTLLEPQSPQSPAGACKKKPSFPEVRASKRENQTAGSPVSGKEIPTGLAKESPVESPVSRTGFQDEPGVTNPKLRELSEGRHLRSPEGTLPRCRERITGIACVLDNQPGLCPLDPIRPRSPTSITASAQVSESCISVEQCEHVVSELQDSMRKALHLYRMVLNDTESSTDKDKIAGLLTETFSSMKKELDSLKDEEELPKVKEVLAKPQDTTSPLNEGCGANLPSPKSLGDEQTLALLEQYSELLLQAVERRMDKKL

Target ORF information:

RefSeq Version	XM_014950326.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014950326.1

ORF Insert Sequence:

ATGATGTCGG TCGAAGGGTC TACCATAACA AGTCGGATCA AAAATCTGCT TCGCTCTCCT 
TCCATTAAGC TGAGGAGAAG CAAGCCTGGG AACAAGCGAG AAGATATAGG CAGCAAGGTG 
ACTTTGGAGA AGGTGTTGGG GATAACAGTG TCAGGAGGCA GAGGATTAGC CTGCGACCCC 
AGAACTGGCC TCGTTGCTTA TCCAGCAGGG TGTGTTGTTG TGCTGTTCAA CCCTAGAAAA 
AACAAGCAAC ACCATATTCT AAACAGTTCC AGGAAAACAA TTACAGCACT TGCTTTCTCT 
CCAGATGGAA AATATTTGGT TACAGGAGAG AGTGGACACA TGCCAGCAGT CCGGGTATGG 
GATGTGGCTG AGCGAACCCA GGTGGCTGAG CTCCAGGAGC ACAAGTATGG CGTGGCCTGT 
GTGGCCTTCT CCCCCAGCTC CAAGTACATT GTTTCAGTGG GGTACCAGCA TGACATGATT 
GTCAACGTCT GGTCATGGAA GAAAAACATT GTGGTGGCAG CCAACAAAGT CTCGAGTAAA 
GTGACAGCCG TGTCATTCTC GGAGGACTGC AGTTACTTTG TCACTGCTGG AAATCGACAC 
ATCAAGTTCT GGTACCTGGA TGACAGCAAA ACCTCCAAGG TCAATGCCAC AGTCCCCTTG 
CTGGGCCGCT CAGGATTGCT TGGAGAACTG AGGAACAACT TCTTTGCGGA CGTGGCGTGT 
GGAAGGGGCA AAAAAGCTGA CAGCACCTTC TGTATCACGT CCTCAGGCCT GCTCTGTGAA 
TTCAATGAAA AAAGGCTGCT GGACAAGTGG GTGGAGCTGA GGAATACAGA CAGCTTCACA 
ACCACTGTGG CAAACTGCAT CTCTGTGAAT CATGACTACA TCTTCTGTGG CTGCGCTGAC 
GGCACCGTGA GGATTTTTAA CCCTCTGAAC CTGCACTTTG TCACCACGCT GCCCAAGCCA 
CACTTCCTGG GAACAGACAT CGCGAGTGTG ACCGATGCCA GTCGTCTTTT CTCTGGTATG 
GCTGATGCGA AGTATCCAGA CACGATTGCC TTGACTTTTG ACCCCACCAA CCAGTGGCTT 
TCCTGTGTAT ACAATGACCA CAGCCTGTAC GTGTGGGATG TCAAAGACCC GAAGAAAGTG 
GGCAAGGTGT ACTCCGCTTT GTACCACTCT TCCTGCGTGT GGAACATTGA GATGTATCCA 
GAGGTGAAGG ACAGCAATCA GTCGTGCCTC CCCCCTGGTT CCTTCATCAC GTGCTCCTCT 
GACAACACCA TTCGCCTGTG GAACACGGAG AGCTCCAACA TTCACGGCAC AGCCCTGCAC 
CGCAACATCC TCAGCAATGA CTTGATGAAA ATCATCTATG TAGATGACAA CACTCAGGTA 
CTTCTGGACA CTGATTACAA CTCGGCAGGA AGTGCAGAGA AAGCTGATGC ACAAGTGATG 
GATACGAAGG TGGGGATCCG CACCGTCTGT GTGAGTCCCA GTGGGGAGCA CCTGGCCTCA 
GGGGACAGGA TAGGCACTCT CAGGATATAT GAGTTGCAGT CTCTGAGGGA AATGCTGAAG 
GTGGAAGCCC ATGACTCTGA GATCCTGTGT CTGGAGTACT CCAAACCTGA TACAGGCTTA 
AAGCTCTTAG CCTCAGCCAG TCGGGATAGA TTGATTCATG TCTTGGATGC TGGAAAGGAC 
TACAGCCTGC AGCAGACCCT GGATGAACAT TCTTCTTCTA TCACTGCTGT GAAGTTTGCA 
GCCAATGATG GGAAAGTGAG AATGATAAGC TGTGGGGCAG ACAAGAGCAT CTATTTCCGC 
ACTGCGCAGA AGACAGGAGA AGGGGTCCAG TTTACCCGAA CACATCACAT TGTTAGGAAG 
ACTACTCTGT ATGACATGGA TGTGGACCCC AGCTGGAAAT ATGCAGCTAT TGGATGCCAG 
GATCGTAACA TCAGGGTTTT CAACATAAGC AGTGGGAAGC AGAAGAAGCT TTACAAAGGA 
TCCCAAGGTG AAGATGGTAC CCTCATCAAA GTGCAAACAG ATCCCTCTGG TCTTTATATT 
GCCACCAGTT GTTCGGACAA GAACCTCTCC ATCTTTGATT TCTATTCTGG CGAGTGTGTT 
GCAACCATGT ATGGACATTC AGAGATTGTA ACCGGGATGA AATTCAGTAA TGACTGCAAA 
CATCTGATCT CTGTTTCTGG TGACAGCTGT ATCTTCATCT GGCGTCTGAG CTCGGAGATG 
ACCATCAACA TGCGGCAGCG ACTGTCTGAC ATGAAGCAGA GAGGGAAGCA GGCAGAAAAA 
TCTCCTCCGC ACAAGACAGC TGGACTCATG AGGCACGAGT CCATCTCTGC CCTGTCCTCT 
GTGCCTGCGC TCTCATCAGA CAGTGACAAG GATGGTGAAG ACGAAGGGAA TGATGAAGAT 
GAGCTACGTG GGCTGCAGTC TTTCCATCTT CAGTCCAACT GCAGCACTGA GAGAGACTCA 
GATCCAGACC TTACTCTCTC AAGAAGCCTT TCCCATTGGG AAATGAGGAG GGCCAAAGAG 
ATAGCGGCGA TCCAGCGCTC AGAGGCACCA ATGAGCAAAA TGCCCCGGCA GCGCGGGCGC 
TGGTCGCAGC CCACCAGCAA CATAGAGATG ACGGTGAAAT CCATGCTTGA CTTGCGTCAG 
TTGGAGTCTT TCTCCATTTC CCGTTCTCCC AGTCGAGACT CACTGTCTCA AAACAGCAAC 
GGGGACGACA GAGAAGAGCA GGCAGATCTT CCTGCACACA TCAACAAAGT TGGCAGCTCA 
GTACCTCCCC AAGAGAACCG GTCTTGTGTG CGACCCCAAG TGATTCGCCT TGTGTCTTGT 
GAAGAGGGGA TTTTCTCTCA GGAACTGGAA CCTTCTGAGA GTGAGGAGTG TGTAATCTAC 
CCTGAACAAG ATGAAATCCA TCCCACAGAC TCTAGCAGTG AGTTCCAGGT AAAAGCACTG 
CCCCACGGGA AGCTCAGTAG AGGTTATCAC AGCAATGGAT GCCCAGACAA ACACAGTCCT 
GACAGTGCCT GCTCTGTAGA TTACAGCAGC AGTCGTCTGT CCAGCCCAGA CCATCCGAAT 
GAAGATTCTG AAAGCACTGA ACCCCTGAGT GTGGATGGTG TCTCAGACCT GGAGGGAGAG 
GAAGGAGAGG AGGACGTGGG TACTACCATG CCGGAAGGAG AAATCCCCCG GACACCCGAT 
CAGGAGAAGT TTCTTAAACA GCATTTTGGG TCCCTGGGCA GCACTGATGG AAAAGGTGGC 
TCATGTAGGA ATTTGGAAAG AACTGAAAAC CTCAGCATTT CCTCTCGCTT TCTTTCCCAG 
TCCTCGGCTC TCAGACAATT GTCACTCTCT TCATCAAATC TGATACTGGA TTCAAAGCCC 
ATTGAGCCGC CAACGCAGAC CAGTCGGCTT ACACCAGACC TGTTGAAAAA TGGCAGCGAC 
CATCCTGGTG ATGCATTGGA GAACGGCCAG CTCCTGGAGA GTGTAAATTC AAATCGGACT 
GTTTATGTCC AGAAAAAACG CAGATCAGCT TTGGAGGCCA GCAGACTTGG TATGGCTCCT 
GGACGGGTCA TCGCATCCTT TCCAGAAGGC CCTGTGAATG CAGTGATGAG AAAGGCACAG 
TCAGTTCACG ACCTCGTTCA TGAGGATAAG GGTCCTGGAG TGGTCTCCTC TGTCAAGCAG 
CGTCCTCAGA CTCTTTTGGT GGTTGAGAAG GATCCCAGAC CTAACAATTG CAGGGTGTTA 
TCAACCAGTA TGTTGAAGAT GGATGGATCT GGTGCTCTGC TGTCCAAAGA TGTCAGGGCT 
GCAAAATCAA AGTCCTACAT GAACCCCACA ACCAGCTTCC GGGCTAAGAT GTCAAGGAGC 
GTATCTGTAG GGGAAAATCT GTACCTCGGT TACTCCGCCG AAATGTTGAC TGATGGGAGG 
ACTAGCCCTC CAGTGGCAGA GAAGACACAG TTTAGTTCTA CAGCAGATGC TGAGAAGATT 
AAGCCACCAG CAAAAGAAAA TGTTCCAGTG AAGCCAGCAC TTCAACCAGT TGTAAACTGC 
TCATCTCCCA AGTCCTTCCA CAGCAAGTTG GCATCGTCAA ACCGAGCACA TCTGATTCTT 
GACATTCCTA AACCATTGCC TGATAGACCC ACACTGGCCT CCTTCTCACC CACTACCAAA 
CCAAAAACCC TACTTGAGCC ACAGTCTCCA CAGTCACCTG CTGGGGCCTG CAAAAAGAAA 
CCCTCTTTTC CTGAGGTCCG AGCCTCCAAG AGGGAGAATC AAACTGCTGG CTCTCCGGTT 
TCTGGTAAAG AGATCCCAAC AGGACTTGCT AAGGAGAGCC CAGTGGAAAG TCCGGTCTCA 
AGGACAGGTT TCCAGGATGA GCCAGGGGTC ACTAATCCAA AACTGAGGGA GTTGTCAGAG 
GGCCGGCACC TAAGGTCTCC TGAAGGTACA CTGCCCAGGT GCAGGGAGAG GATCACCGGC 
ATCGCCTGTG TATTAGACAA CCAACCTGGA CTTTGCCCAC TAGACCCCAT CAGGCCGAGG 
TCTCCTACAT CTATAACGGC CTCGGCACAG GTCTCAGAAT CGTGCATCAG CGTGGAGCAG 
TGTGAGCACG TGGTGTCCGA GCTCCAGGAC AGCATGCGCA AAGCCCTTCA TCTGTACCGC 
ATGGTGTTAA ATGATACGGA GTCTTCTACT GACAAAGATA AAATAGCGGG CCTCCTGACA 
GAAACATTCT CCTCAATGAA GAAAGAATTG GACTCTCTGA AGGATGAGGA GGAGCTTCCA 
AAGGTTAAGG AGGTACTAGC GAAGCCACAA GATACCACTA GCCCACTGAA TGAGGGATGT 
GGTGCCAACC TACCGAGTCC CAAGAGTTTG GGAGATGAGC AGACACTAGC TTTGCTGGAG 
CAGTACTCAG AGCTGTTGCT ACAAGCTGTG GAACGACGCA TGGACAAAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPKBP1 ( XM_014950327.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014805813.1 Calidris pugnax mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa71164
Clone ID Related Accession (Same CDS sequence)	XM_014950327.1
Accession Version	XM_014950327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG TCGAAGGGTC TACCATAACA AGTCGGATCA AAAATCTGCT TCGCTCTCCT 
TCCATTAAGC TGAGGAGAAG CAAGCCTGGG AACAAGCGAG AAGATATAGG CAGCAAGGTG 
ACTTTGGAGA AGGTGTTGGG GATAACAGTG TCAGGAGGCA GAGGATTAGC CTGCGACCCC 
AGAACTGGCC TCGTTGCTTA TCCAGCAGGG TGTGTTGTTG TGCTGTTCAA CCCTAGAAAA 
AACAAGCAAC ACCATATTCT AAACAGTTCC AGGAAAACAA TTACAGCACT TGCTTTCTCT 
CCAGATGGAA AATATTTGGT TACAGGAGAG AGTGGACACA TGCCAGCAGT CCGGGTATGG 
GATGTGGCTG AGCGAACCCA GGTGGCTGAG CTCCAGGAGC ACAAGTATGG CGTGGCCTGT 
GTGGCCTTCT CCCCCAGCTC CAAGTACATT GTTTCAGTGG GGTACCAGCA TGACATGATT 
GTCAACGTCT GGTCATGGAA GAAAAACATT GTGGTGGCAG CCAACAAAGT CTCGAGTAAA 
GTGACAGCCG TGTCATTCTC GGAGGACTGC AGTTACTTTG TCACTGCTGG AAATCGACAC 
ATCAAGTTCT GGTACCTGGA TGACAGCAAA ACCTCCAAGG TCAATGCCAC AGTCCCCTTG 
CTGGGCCGCT CAGGATTGCT TGGAGAACTG AGGAACAACT TCTTTGCGGA CGTGGCGTGT 
GGAAGGGGCA AAAAAGCTGA CAGCACCTTC TGTATCACGT CCTCAGGCCT GCTCTGTGAA 
TTCAATGAAA AAAGGCTGCT GGACAAGTGG GTGGAGCTGA GGACCACTGT GGCAAACTGC 
ATCTCTGTGA ATCATGACTA CATCTTCTGT GGCTGCGCTG ACGGCACCGT GAGGATTTTT 
AACCCTCTGA ACCTGCACTT TGTCACCACG CTGCCCAAGC CACACTTCCT GGGAACAGAC 
ATCGCGAGTG TGACCGATGC CAGTCGTCTT TTCTCTGGTA TGGCTGATGC GAAGTATCCA 
GACACGATTG CCTTGACTTT TGACCCCACC AACCAGTGGC TTTCCTGTGT ATACAATGAC 
CACAGCCTGT ACGTGTGGGA TGTCAAAGAC CCGAAGAAAG TGGGCAAGGT GTACTCCGCT 
TTGTACCACT CTTCCTGCGT GTGGAACATT GAGATGTATC CAGAGGTGAA GGACAGCAAT 
CAGTCGTGCC TCCCCCCTGG TTCCTTCATC ACGTGCTCCT CTGACAACAC CATTCGCCTG 
TGGAACACGG AGAGCTCCAA CATTCACGGC ACAGCCCTGC ACCGCAACAT CCTCAGCAAT 
GACTTGATGA AAATCATCTA TGTAGATGAC AACACTCAGG TACTTCTGGA CACTGATTAC 
AACTCGGCAG GAAGTGCAGA GAAAGCTGAT GCACAAGTGA TGGATACGAA GGTGGGGATC 
CGCACCGTCT GTGTGAGTCC CAGTGGGGAG CACCTGGCCT CAGGGGACAG GATAGGCACT 
CTCAGGATAT ATGAGTTGCA GTCTCTGAGG GAAATGCTGA AGGTGGAAGC CCATGACTCT 
GAGATCCTGT GTCTGGAGTA CTCCAAACCT GATACAGGCT TAAAGCTCTT AGCCTCAGCC 
AGTCGGGATA GATTGATTCA TGTCTTGGAT GCTGGAAAGG ACTACAGCCT GCAGCAGACC 
CTGGATGAAC ATTCTTCTTC TATCACTGCT GTGAAGTTTG CAGCCAATGA TGGGAAAGTG 
AGAATGATAA GCTGTGGGGC AGACAAGAGC ATCTATTTCC GCACTGCGCA GAAGACAGGA 
GAAGGGGTCC AGTTTACCCG AACACATCAC ATTGTTAGGA AGACTACTCT GTATGACATG 
GATGTGGACC CCAGCTGGAA ATATGCAGCT ATTGGATGCC AGGATCGTAA CATCAGGGTT 
TTCAACATAA GCAGTGGGAA GCAGAAGAAG CTTTACAAAG GATCCCAAGG TGAAGATGGT 
ACCCTCATCA AAGTGCAAAC AGATCCCTCT GGTCTTTATA TTGCCACCAG TTGTTCGGAC 
AAGAACCTCT CCATCTTTGA TTTCTATTCT GGCGAGTGTG TTGCAACCAT GTATGGACAT 
TCAGAGATTG TAACCGGGAT GAAATTCAGT AATGACTGCA AACATCTGAT CTCTGTTTCT 
GGTGACAGCT GTATCTTCAT CTGGCGTCTG AGCTCGGAGA TGACCATCAA CATGCGGCAG 
CGACTGTCTG ACATGAAGCA GAGAGGGAAG CAGGCAGAAA AATCTCCTCC GCACAAGACA 
GCTGGACTCA TGAGGCACGA GTCCATCTCT GCCCTGTCCT CTGTGCCTGC GCTCTCATCA 
GACAGTGACA AGGATGGTGA AGACGAAGGG AATGATGAAG ATGAGCTACG TGGGCTGCAG 
TCTTTCCATC TTCAGTCCAA CTGCAGCACT GAGAGAGACT CAGATCCAGA CCTTACTCTC 
TCAAGAAGCC TTTCCCATTG GGAAATGAGG AGGGCCAAAG AGATAGCGGC GATCCAGCGC 
TCAGAGGCAC CAATGAGCAA AATGCCCCGG CAGCGCGGGC GCTGGTCGCA GCCCACCAGC 
AACATAGAGA TGACGGTGAA ATCCATGCTT GACTTGCGTC AGTTGGAGTC TTTCTCCATT 
TCCCGTTCTC CCAGTCGAGA CTCACTGTCT CAAAACAGCA ACGGGGACGA CAGAGAAGAG 
CAGGCAGATC TTCCTGCACA CATCAACAAA GTTGGCAGCT CAGTACCTCC CCAAGAGAAC 
CGGTCTTGTG TGCGACCCCA AGTGATTCGC CTTGTGTCTT GTGAAGAGGG GATTTTCTCT 
CAGGAACTGG AACCTTCTGA GAGTGAGGAG TGTGTAATCT ACCCTGAACA AGATGAAATC 
CATCCCACAG ACTCTAGCAG TGAGTTCCAG GTAAAAGCAC TGCCCCACGG GAAGCTCAGT 
AGAGGTTATC ACAGCAATGG ATGCCCAGAC AAACACAGTC CTGACAGTGC CTGCTCTGTA 
GATTACAGCA GCAGTCGTCT GTCCAGCCCA GACCATCCGA ATGAAGATTC TGAAAGCACT 
GAACCCCTGA GTGTGGATGG TGTCTCAGAC CTGGAGGGAG AGGAAGGAGA GGAGGACGTG 
GGTACTACCA TGCCGGAAGG AGAAATCCCC CGGACACCCG ATCAGGAGAA GTTTCTTAAA 
CAGCATTTTG GGTCCCTGGG CAGCACTGAT GGAAAAGGTG GCTCATGTAG GAATTTGGAA 
AGAACTGAAA ACCTCAGCAT TTCCTCTCGC TTTCTTTCCC AGTCCTCGGC TCTCAGACAA 
TTGTCACTCT CTTCATCAAA TCTGATACTG GATTCAAAGC CCATTGAGCC GCCAACGCAG 
ACCAGTCGGC TTACACCAGA CCTGTTGAAA AATGGCAGCG ACCATCCTGG TGATGCATTG 
GAGAACGGCC AGCTCCTGGA GAGTGTAAAT TCAAATCGGA CTGTTTATGT CCAGAAAAAA 
CGCAGATCAG CTTTGGAGGC CAGCAGACTT GGTATGGCTC CTGGACGGGT CATCGCATCC 
TTTCCAGAAG GCCCTGTGAA TGCAGTGATG AGAAAGGCAC AGTCAGTTCA CGACCTCGTT 
CATGAGGATA AGGGTCCTGG AGTGGTCTCC TCTGTCAAGC AGCGTCCTCA GACTCTTTTG 
GTGGTTGAGA AGGATCCCAG ACCTAACAAT TGCAGGGTGT TATCAACCAG TATGTTGAAG 
ATGGATGGAT CTGGTGCTCT GCTGTCCAAA GATGTCAGGG CTGCAAAATC AAAGTCCTAC 
ATGAACCCCA CAACCAGCTT CCGGGCTAAG ATGTCAAGGA GCGTATCTGT AGGGGAAAAT 
CTGTACCTCG GTTACTCCGC CGAAATGTTG ACTGATGGGA GGACTAGCCC TCCAGTGGCA 
GAGAAGACAC AGTTTAGTTC TACAGCAGAT GCTGAGAAGA TTAAGCCACC AGCAAAAGAA 
AATGTTCCAG TGAAGCCAGC ACTTCAACCA GTTGTAAACT GCTCATCTCC CAAGTCCTTC 
CACAGCAAGT TGGCATCGTC AAACCGAGCA CATCTGATTC TTGACATTCC TAAACCATTG 
CCTGATAGAC CCACACTGGC CTCCTTCTCA CCCACTACCA AACCAAAAAC CCTACTTGAG 
CCACAGTCTC CACAGTCACC TGCTGGGGCC TGCAAAAAGA AACCCTCTTT TCCTGAGGTC 
CGAGCCTCCA AGAGGGAGAA TCAAACTGCT GGCTCTCCGG TTTCTGGTAA AGAGATCCCA 
ACAGGACTTG CTAAGGAGAG CCCAGTGGAA AGTCCGGTCT CAAGGACAGG TTTCCAGGAT 
GAGCCAGGGG TCACTAATCC AAAACTGAGG GAGTTGTCAG AGGGCCGGCA CCTAAGGTCT 
CCTGAAGGTA CACTGCCCAG GTGCAGGGAG AGGATCACCG GCATCGCCTG TGTATTAGAC 
AACCAACCTG GACTTTGCCC ACTAGACCCC ATCAGGCCGA GGTCTCCTAC ATCTATAACG 
GCCTCGGCAC AGGTCTCAGA ATCGTGCATC AGCGTGGAGC AGTGTGAGCA CGTGGTGTCC 
GAGCTCCAGG ACAGCATGCG CAAAGCCCTT CATCTGTACC GCATGGTGTT AAATGATACG 
GAGTCTTCTA CTGACAAAGA TAAAATAGCG GGCCTCCTGA CAGAAACATT CTCCTCAATG 
AAGAAAGAAT TGGACTCTCT GAAGGATGAG GAGGAGCTTC CAAAGGTTAA GGAGGTACTA 
GCGAAGCCAC AAGATACCAC TAGCCCACTG AATGAGGGAT GTGGTGCCAA CCTACCGAGT 
CCCAAGAGTT TGGGAGATGA GCAGACACTA GCTTTGCTGG AGCAGTACTC AGAGCTGTTG 
CTACAAGCTG TGGAACGACG CATGGACAAA AAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014805813.1
CDS	220..5118
Translation	MMSVEGSTITSRIKNLLRSPSIKLRRSKPGNKREDIGSKVTLEKVLGITVSGGRGLACDPRTGLVAYPAGCVVVLFNPRKNKQHHILNSSRKTITALAFSPDGKYLVTGESGHMPAVRVWDVAERTQVAELQEHKYGVACVAFSPSSKYIVSVGYQHDMIVNVWSWKKNIVVAANKVSSKVTAVSFSEDCSYFVTAGNRHIKFWYLDDSKTSKVNATVPLLGRSGLLGELRNNFFADVACGRGKKADSTFCITSSGLLCEFNEKRLLDKWVELRTTVANCISVNHDYIFCGCADGTVRIFNPLNLHFVTTLPKPHFLGTDIASVTDASRLFSGMADAKYPDTIALTFDPTNQWLSCVYNDHSLYVWDVKDPKKVGKVYSALYHSSCVWNIEMYPEVKDSNQSCLPPGSFITCSSDNTIRLWNTESSNIHGTALHRNILSNDLMKIIYVDDNTQVLLDTDYNSAGSAEKADAQVMDTKVGIRTVCVSPSGEHLASGDRIGTLRIYELQSLREMLKVEAHDSEILCLEYSKPDTGLKLLASASRDRLIHVLDAGKDYSLQQTLDEHSSSITAVKFAANDGKVRMISCGADKSIYFRTAQKTGEGVQFTRTHHIVRKTTLYDMDVDPSWKYAAIGCQDRNIRVFNISSGKQKKLYKGSQGEDGTLIKVQTDPSGLYIATSCSDKNLSIFDFYSGECVATMYGHSEIVTGMKFSNDCKHLISVSGDSCIFIWRLSSEMTINMRQRLSDMKQRGKQAEKSPPHKTAGLMRHESISALSSVPALSSDSDKDGEDEGNDEDELRGLQSFHLQSNCSTERDSDPDLTLSRSLSHWEMRRAKEIAAIQRSEAPMSKMPRQRGRWSQPTSNIEMTVKSMLDLRQLESFSISRSPSRDSLSQNSNGDDREEQADLPAHINKVGSSVPPQENRSCVRPQVIRLVSCEEGIFSQELEPSESEECVIYPEQDEIHPTDSSSEFQVKALPHGKLSRGYHSNGCPDKHSPDSACSVDYSSSRLSSPDHPNEDSESTEPLSVDGVSDLEGEEGEEDVGTTMPEGEIPRTPDQEKFLKQHFGSLGSTDGKGGSCRNLERTENLSISSRFLSQSSALRQLSLSSSNLILDSKPIEPPTQTSRLTPDLLKNGSDHPGDALENGQLLESVNSNRTVYVQKKRRSALEASRLGMAPGRVIASFPEGPVNAVMRKAQSVHDLVHEDKGPGVVSSVKQRPQTLLVVEKDPRPNNCRVLSTSMLKMDGSGALLSKDVRAAKSKSYMNPTTSFRAKMSRSVSVGENLYLGYSAEMLTDGRTSPPVAEKTQFSSTADAEKIKPPAKENVPVKPALQPVVNCSSPKSFHSKLASSNRAHLILDIPKPLPDRPTLASFSPTTKPKTLLEPQSPQSPAGACKKKPSFPEVRASKRENQTAGSPVSGKEIPTGLAKESPVESPVSRTGFQDEPGVTNPKLRELSEGRHLRSPEGTLPRCRERITGIACVLDNQPGLCPLDPIRPRSPTSITASAQVSESCISVEQCEHVVSELQDSMRKALHLYRMVLNDTESSTDKDKIAGLLTETFSSMKKELDSLKDEEELPKVKEVLAKPQDTTSPLNEGCGANLPSPKSLGDEQTLALLEQYSELLLQAVERRMDKKL

Target ORF information:

RefSeq Version	XM_014950327.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014950327.1

ORF Insert Sequence:

ATGATGTCGG TCGAAGGGTC TACCATAACA AGTCGGATCA AAAATCTGCT TCGCTCTCCT 
TCCATTAAGC TGAGGAGAAG CAAGCCTGGG AACAAGCGAG AAGATATAGG CAGCAAGGTG 
ACTTTGGAGA AGGTGTTGGG GATAACAGTG TCAGGAGGCA GAGGATTAGC CTGCGACCCC 
AGAACTGGCC TCGTTGCTTA TCCAGCAGGG TGTGTTGTTG TGCTGTTCAA CCCTAGAAAA 
AACAAGCAAC ACCATATTCT AAACAGTTCC AGGAAAACAA TTACAGCACT TGCTTTCTCT 
CCAGATGGAA AATATTTGGT TACAGGAGAG AGTGGACACA TGCCAGCAGT CCGGGTATGG 
GATGTGGCTG AGCGAACCCA GGTGGCTGAG CTCCAGGAGC ACAAGTATGG CGTGGCCTGT 
GTGGCCTTCT CCCCCAGCTC CAAGTACATT GTTTCAGTGG GGTACCAGCA TGACATGATT 
GTCAACGTCT GGTCATGGAA GAAAAACATT GTGGTGGCAG CCAACAAAGT CTCGAGTAAA 
GTGACAGCCG TGTCATTCTC GGAGGACTGC AGTTACTTTG TCACTGCTGG AAATCGACAC 
ATCAAGTTCT GGTACCTGGA TGACAGCAAA ACCTCCAAGG TCAATGCCAC AGTCCCCTTG 
CTGGGCCGCT CAGGATTGCT TGGAGAACTG AGGAACAACT TCTTTGCGGA CGTGGCGTGT 
GGAAGGGGCA AAAAAGCTGA CAGCACCTTC TGTATCACGT CCTCAGGCCT GCTCTGTGAA 
TTCAATGAAA AAAGGCTGCT GGACAAGTGG GTGGAGCTGA GGACCACTGT GGCAAACTGC 
ATCTCTGTGA ATCATGACTA CATCTTCTGT GGCTGCGCTG ACGGCACCGT GAGGATTTTT 
AACCCTCTGA ACCTGCACTT TGTCACCACG CTGCCCAAGC CACACTTCCT GGGAACAGAC 
ATCGCGAGTG TGACCGATGC CAGTCGTCTT TTCTCTGGTA TGGCTGATGC GAAGTATCCA 
GACACGATTG CCTTGACTTT TGACCCCACC AACCAGTGGC TTTCCTGTGT ATACAATGAC 
CACAGCCTGT ACGTGTGGGA TGTCAAAGAC CCGAAGAAAG TGGGCAAGGT GTACTCCGCT 
TTGTACCACT CTTCCTGCGT GTGGAACATT GAGATGTATC CAGAGGTGAA GGACAGCAAT 
CAGTCGTGCC TCCCCCCTGG TTCCTTCATC ACGTGCTCCT CTGACAACAC CATTCGCCTG 
TGGAACACGG AGAGCTCCAA CATTCACGGC ACAGCCCTGC ACCGCAACAT CCTCAGCAAT 
GACTTGATGA AAATCATCTA TGTAGATGAC AACACTCAGG TACTTCTGGA CACTGATTAC 
AACTCGGCAG GAAGTGCAGA GAAAGCTGAT GCACAAGTGA TGGATACGAA GGTGGGGATC 
CGCACCGTCT GTGTGAGTCC CAGTGGGGAG CACCTGGCCT CAGGGGACAG GATAGGCACT 
CTCAGGATAT ATGAGTTGCA GTCTCTGAGG GAAATGCTGA AGGTGGAAGC CCATGACTCT 
GAGATCCTGT GTCTGGAGTA CTCCAAACCT GATACAGGCT TAAAGCTCTT AGCCTCAGCC 
AGTCGGGATA GATTGATTCA TGTCTTGGAT GCTGGAAAGG ACTACAGCCT GCAGCAGACC 
CTGGATGAAC ATTCTTCTTC TATCACTGCT GTGAAGTTTG CAGCCAATGA TGGGAAAGTG 
AGAATGATAA GCTGTGGGGC AGACAAGAGC ATCTATTTCC GCACTGCGCA GAAGACAGGA 
GAAGGGGTCC AGTTTACCCG AACACATCAC ATTGTTAGGA AGACTACTCT GTATGACATG 
GATGTGGACC CCAGCTGGAA ATATGCAGCT ATTGGATGCC AGGATCGTAA CATCAGGGTT 
TTCAACATAA GCAGTGGGAA GCAGAAGAAG CTTTACAAAG GATCCCAAGG TGAAGATGGT 
ACCCTCATCA AAGTGCAAAC AGATCCCTCT GGTCTTTATA TTGCCACCAG TTGTTCGGAC 
AAGAACCTCT CCATCTTTGA TTTCTATTCT GGCGAGTGTG TTGCAACCAT GTATGGACAT 
TCAGAGATTG TAACCGGGAT GAAATTCAGT AATGACTGCA AACATCTGAT CTCTGTTTCT 
GGTGACAGCT GTATCTTCAT CTGGCGTCTG AGCTCGGAGA TGACCATCAA CATGCGGCAG 
CGACTGTCTG ACATGAAGCA GAGAGGGAAG CAGGCAGAAA AATCTCCTCC GCACAAGACA 
GCTGGACTCA TGAGGCACGA GTCCATCTCT GCCCTGTCCT CTGTGCCTGC GCTCTCATCA 
GACAGTGACA AGGATGGTGA AGACGAAGGG AATGATGAAG ATGAGCTACG TGGGCTGCAG 
TCTTTCCATC TTCAGTCCAA CTGCAGCACT GAGAGAGACT CAGATCCAGA CCTTACTCTC 
TCAAGAAGCC TTTCCCATTG GGAAATGAGG AGGGCCAAAG AGATAGCGGC GATCCAGCGC 
TCAGAGGCAC CAATGAGCAA AATGCCCCGG CAGCGCGGGC GCTGGTCGCA GCCCACCAGC 
AACATAGAGA TGACGGTGAA ATCCATGCTT GACTTGCGTC AGTTGGAGTC TTTCTCCATT 
TCCCGTTCTC CCAGTCGAGA CTCACTGTCT CAAAACAGCA ACGGGGACGA CAGAGAAGAG 
CAGGCAGATC TTCCTGCACA CATCAACAAA GTTGGCAGCT CAGTACCTCC CCAAGAGAAC 
CGGTCTTGTG TGCGACCCCA AGTGATTCGC CTTGTGTCTT GTGAAGAGGG GATTTTCTCT 
CAGGAACTGG AACCTTCTGA GAGTGAGGAG TGTGTAATCT ACCCTGAACA AGATGAAATC 
CATCCCACAG ACTCTAGCAG TGAGTTCCAG GTAAAAGCAC TGCCCCACGG GAAGCTCAGT 
AGAGGTTATC ACAGCAATGG ATGCCCAGAC AAACACAGTC CTGACAGTGC CTGCTCTGTA 
GATTACAGCA GCAGTCGTCT GTCCAGCCCA GACCATCCGA ATGAAGATTC TGAAAGCACT 
GAACCCCTGA GTGTGGATGG TGTCTCAGAC CTGGAGGGAG AGGAAGGAGA GGAGGACGTG 
GGTACTACCA TGCCGGAAGG AGAAATCCCC CGGACACCCG ATCAGGAGAA GTTTCTTAAA 
CAGCATTTTG GGTCCCTGGG CAGCACTGAT GGAAAAGGTG GCTCATGTAG GAATTTGGAA 
AGAACTGAAA ACCTCAGCAT TTCCTCTCGC TTTCTTTCCC AGTCCTCGGC TCTCAGACAA 
TTGTCACTCT CTTCATCAAA TCTGATACTG GATTCAAAGC CCATTGAGCC GCCAACGCAG 
ACCAGTCGGC TTACACCAGA CCTGTTGAAA AATGGCAGCG ACCATCCTGG TGATGCATTG 
GAGAACGGCC AGCTCCTGGA GAGTGTAAAT TCAAATCGGA CTGTTTATGT CCAGAAAAAA 
CGCAGATCAG CTTTGGAGGC CAGCAGACTT GGTATGGCTC CTGGACGGGT CATCGCATCC 
TTTCCAGAAG GCCCTGTGAA TGCAGTGATG AGAAAGGCAC AGTCAGTTCA CGACCTCGTT 
CATGAGGATA AGGGTCCTGG AGTGGTCTCC TCTGTCAAGC AGCGTCCTCA GACTCTTTTG 
GTGGTTGAGA AGGATCCCAG ACCTAACAAT TGCAGGGTGT TATCAACCAG TATGTTGAAG 
ATGGATGGAT CTGGTGCTCT GCTGTCCAAA GATGTCAGGG CTGCAAAATC AAAGTCCTAC 
ATGAACCCCA CAACCAGCTT CCGGGCTAAG ATGTCAAGGA GCGTATCTGT AGGGGAAAAT 
CTGTACCTCG GTTACTCCGC CGAAATGTTG ACTGATGGGA GGACTAGCCC TCCAGTGGCA 
GAGAAGACAC AGTTTAGTTC TACAGCAGAT GCTGAGAAGA TTAAGCCACC AGCAAAAGAA 
AATGTTCCAG TGAAGCCAGC ACTTCAACCA GTTGTAAACT GCTCATCTCC CAAGTCCTTC 
CACAGCAAGT TGGCATCGTC AAACCGAGCA CATCTGATTC TTGACATTCC TAAACCATTG 
CCTGATAGAC CCACACTGGC CTCCTTCTCA CCCACTACCA AACCAAAAAC CCTACTTGAG 
CCACAGTCTC CACAGTCACC TGCTGGGGCC TGCAAAAAGA AACCCTCTTT TCCTGAGGTC 
CGAGCCTCCA AGAGGGAGAA TCAAACTGCT GGCTCTCCGG TTTCTGGTAA AGAGATCCCA 
ACAGGACTTG CTAAGGAGAG CCCAGTGGAA AGTCCGGTCT CAAGGACAGG TTTCCAGGAT 
GAGCCAGGGG TCACTAATCC AAAACTGAGG GAGTTGTCAG AGGGCCGGCA CCTAAGGTCT 
CCTGAAGGTA CACTGCCCAG GTGCAGGGAG AGGATCACCG GCATCGCCTG TGTATTAGAC 
AACCAACCTG GACTTTGCCC ACTAGACCCC ATCAGGCCGA GGTCTCCTAC ATCTATAACG 
GCCTCGGCAC AGGTCTCAGA ATCGTGCATC AGCGTGGAGC AGTGTGAGCA CGTGGTGTCC 
GAGCTCCAGG ACAGCATGCG CAAAGCCCTT CATCTGTACC GCATGGTGTT AAATGATACG 
GAGTCTTCTA CTGACAAAGA TAAAATAGCG GGCCTCCTGA CAGAAACATT CTCCTCAATG 
AAGAAAGAAT TGGACTCTCT GAAGGATGAG GAGGAGCTTC CAAAGGTTAA GGAGGTACTA 
GCGAAGCCAC AAGATACCAC TAGCCCACTG AATGAGGGAT GTGGTGCCAA CCTACCGAGT 
CCCAAGAGTT TGGGAGATGA GCAGACACTA GCTTTGCTGG AGCAGTACTC AGAGCTGTTG 
CTACAAGCTG TGGAACGACG CATGGACAAA AAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPKBP1 ( XM_014950328.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014805814.1 Calidris pugnax mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant X3, mRNA.

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CloneID	OCa71165
Clone ID Related Accession (Same CDS sequence)	XM_014950328.1
Accession Version	XM_014950328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase-binding protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGG TCGAAGGGTC TACCATAACA AGTCGGATCA AAAATCTGCT TCGCTCTCCT 
TCCATTAAGC TGAGGAGAAG CAAGCCTGGG AACAAGCGAG AAGATATAGG CAGCAAGGTG 
ACTTTGGAGA AGGTGTTGGG GATAACAGTG TCAGGAGGCA GAGGATTAGC CTGCGACCCC 
AGAACTGGCC TCGTTGCTTA TCCAGCAGGG TGTGTTGTTG TGCTGTTCAA CCCTAGAAAA 
AACAAGCAAC ACCATATTCT AAACAGTTCC AGGAAAACAA TTACAGCACT TGCTTTCTCT 
CCAGATGGAA AATATTTGGT TACAGGAGAG AGTGGACACA TGCCAGCAGT CCGGGTATGG 
GATGTGGCTG AGCGAACCCA GGTGGCTGAG CTCCAGGAGC ACAAGTATGG CGTGGCCTGT 
GTGGCCTTCT CCCCCAGCTC CAAGTACATT GTTTCAGTGG GGTACCAGCA TGACATGATT 
GTCAACGTCT GGTCATGGAA GAAAAACATT GTGGTGGCAG CCAACAAAGT CTCGAGTAAA 
GTGACAGCCG TGTCATTCTC GGAGGACTGC AGTTACTTTG TCACTGCTGG AAATCGACAC 
ATCAAGTTCT GGTACCTGGA TGACAGCAAA ACCTCCAAGG TCAATGCCAC AGTCCCCTTG 
CTGGGCCGCT CAGGATTGCT TGGAGAACTG AGGAACAACT TCTTTGCGGA CGTGGCGTGT 
GGAAGGGGCA AAAAAGCTGA CAGCACCTTC TGTATCACGT CCTCAGGCCT GCTCTGTGAA 
TTCAATGAAA AAAGGCTGCT GGACAAGTGG GTGGAGCTGA GGAATACAGA CAGCTTCACA 
ACCACTGTGG CAAACTGCAT CTCTGTGAAT CATGACTACA TCTTCTGTGG CTGCGCTGAC 
GGCACCGTGA GGATTTTTAA CCCTCTGAAC CTGCACTTTG TCACCACGCT GCCCAAGCCA 
CACTTCCTGG GAACAGACAT CGCGAGTGTG ACCGATGCCA GTCGTCTTTT CTCTGGTATG 
GCTGATGCGA AGTATCCAGA CACGATTGCC TTGACTTTTG ACCCCACCAA CCAGTGGCTT 
TCCTGTGTAT ACAATGACCA CAGCCTGTAC GTGTGGGATG TCAAAGACCC GAAGAAAGTG 
GGCAAGGTGT ACTCCGCTTT GTACCACTCT TCCTGCGTGT GGAACATTGA GATGTATCCA 
GAGGTGAAGG ACAGCAATCA GTCGTGCCTC CCCCCTGGTT CCTTCATCAC GTGCTCCTCT 
GACAACACCA TTCGCCTGTG GAACACGGAG AGCTCCAACA TTCACGGCAC AGCCCTGCAC 
CGCAACATCC TCAGCAATGA CTTGATGAAA ATCATCTATG TAGATGACAA CACTCAGGTA 
CTTCTGGACA CTGATTACAA CTCGGCAGGA AGTGCAGAGA AAGCTGATGC ACAAGTGATG 
GATACGAAGG TGGGGATCCG CACCGTCTGT GTGAGTCCCA GTGGGGAGCA CCTGGCCTCA 
GGGGACAGGA TAGGCACTCT CAGGATATAT GAGTTGCAGT CTCTGAGGGA AATGCTGAAG 
GTGGAAGCCC ATGACTCTGA GATCCTGTGT CTGGAGTACT CCAAACCTGA TACAGGCTTA 
AAGCTCTTAG CCTCAGCCAG TCGGGATAGA TTGATTCATG TCTTGGATGC TGGAAAGGAC 
TACAGCCTGC AGCAGACCCT GGATGAACAT TCTTCTTCTA TCACTGCTGT GAAGTTTGCA 
GCCAATGATG GGAAAGTGAG AATGATAAGC TGTGGGGCAG ACAAGAGCAT CTATTTCCGC 
ACTGCGCAGA AGACAGGAGA AGGGGTCCAG TTTACCCGAA CACATCACAT TGTTAGGAAG 
ACTACTCTGT ATGACATGGA TGTGGACCCC AGCTGGAAAT ATGCAGCTAT TGGATGCCAG 
GATCGTAACA TCAGGGTTTT CAACATAAGC AGTGGGAAGC AGAAGAAGCT TTACAAAGGA 
TCCCAAGGTG AAGATGGTAC CCTCATCAAA GTGCAAACAG ATCCCTCTGG TCTTTATATT 
GCCACCAGTT GTTCGGACAA GAACCTCTCC ATCTTTGATT TCTATTCTGG CGAGTGTGTT 
GCAACCATGT ATGGACATTC AGAGATTGTA ACCGGGATGA AATTCAGTAA TGACTGCAAA 
CATCTGATCT CTGTTTCTGG TGACAGCTGT ATCTTCATCT GGCGTCTGAG CTCGGAGATG 
ACCATCAACA TGCGGCAGCG ACTGTCTGAC ATGAAGCAGA GAGGGAAGCA GGCAGAAAAA 
TCTCCTCCGC ACAAGACAGC TGGACTCATG AGGCACGAGT CCATCTCTGC CCTGTCCTCT 
GTGCCTGCGC TCTCATCAGA CAGTGACAAG GATGGTGAAG ACGAAGGGAA TGATGAAGAT 
GAGCTACGTG GGCTGCAGTC TTTCCATCTT CAGTCCAACT GCAGCACTGA GAGAGACTCA 
GATCCAGACC TTACTCTCTC AAGAAGCCTT TCCCATTGGG AAATGAGGAG GGCCAAAGAG 
ATAGCGGCGA TCCAGCGCTC AGAGGCACCA ATGAGCAAAA TGCCCCGGCA GCGCGGGCGC 
TGGTCGCAGC CCACCAGCAA CATAGAGATG ACGGTGAAAT CCATGCTTGA CTTGCGTCAG 
TTGGAGTCTT TCTCCATTTC CCGTTCTCCC AGTCGAGACT CACTGTCTCA AAACAGCAAC 
GGGGACGACA GAGAAGAGCA GGCAGATCTT CCTGCACACA TCAACAAAGT TGGCAGCTCA 
GTACCTCCCC AAGAGAACCG GTCTTGTGTG CGACCCCAAG TGATTCGCCT TGTGTCTTGT 
GAAGAGGGGA TTTTCTCTCA GGAACTGGAA CCTTCTGAGA GTGAGGAGTG TGTAATCTAC 
CCTGAACAAG ATGAAATCCA TCCCACAGAC TCTAGCAGAC AATTGTCACT CTCTTCATCA 
AATCTGATAC TGGATTCAAA GCCCATTGAG CCGCCAACGC AGACCAGTCG GCTTACACCA 
GACCTGTTGA AAAATGGCAG CGACCATCCT GGTGATGCAT TGGAGAACGG CCAGCTCCTG 
GAGAGTGTAA ATTCAAATCG GACTGTTTAT GTCCAGAAAA AACGCAGATC AGCTTTGGAG 
GCCAGCAGAC TTGGTATGGC TCCTGGACGG GTCATCGCAT CCTTTCCAGA AGGCCCTGTG 
AATGCAGTGA TGAGAAAGGC ACAGTCAGTT CACGACCTCG TTCATGAGGA TAAGGGTCCT 
GGAGTGGTCT CCTCTGTCAA GCAGCGTCCT CAGACTCTTT TGGTGGTTGA GAAGGATCCC 
AGACCTAACA ATTGCAGGGT GTTATCAACC AGTATGTTGA AGATGGATGG ATCTGGTGCT 
CTGCTGTCCA AAGATGTCAG GGCTGCAAAA TCAAAGTCCT ACATGAACCC CACAACCAGC 
TTCCGGGCTA AGATGTCAAG GAGCGTATCT GTAGGGGAAA ATCTGTACCT CGGTTACTCC 
GCCGAAATGT TGACTGATGG GAGGACTAGC CCTCCAGTGG CAGAGAAGAC ACAGTTTAGT 
TCTACAGCAG ATGCTGAGAA GATTAAGCCA CCAGCAAAAG AAAATGTTCC AGTGAAGCCA 
GCACTTCAAC CAGTTGTAAA CTGCTCATCT CCCAAGTCCT TCCACAGCAA GTTGGCATCG 
TCAAACCGAG CACATCTGAT TCTTGACATT CCTAAACCAT TGCCTGATAG ACCCACACTG 
GCCTCCTTCT CACCCACTAC CAAACCAAAA ACCCTACTTG AGCCACAGTC TCCACAGTCA 
CCTGCTGGGG CCTGCAAAAA GAAACCCTCT TTTCCTGAGG TCCGAGCCTC CAAGAGGGAG 
AATCAAACTG CTGGCTCTCC GGTTTCTGGT AAAGAGATCC CAACAGGACT TGCTAAGGAG 
AGCCCAGTGG AAAGTCCGGT CTCAAGGACA GGTTTCCAGG ATGAGCCAGG GGTCACTAAT 
CCAAAACTGA GGGAGTTGTC AGAGGGCCGG CACCTAAGGT CTCCTGAAGG TACACTGCCC 
AGGTGCAGGG AGAGGATCAC CGGCATCGCC TGTGTATTAG ACAACCAACC TGGACTTTGC 
CCACTAGACC CCATCAGGCC GAGGTCTCCT ACATCTATAA CGGCCTCGGC ACAGGTCTCA 
GAATCGTGCA TCAGCGTGGA GCAGTGTGAG CACGTGGTGT CCGAGCTCCA GGACAGCATG 
CGCAAAGCCC TTCATCTGTA CCGCATGGTG TTAAATGATA CGGAGTCTTC TACTGACAAA 
GATAAAATAG CGGGCCTCCT GACAGAAACA TTCTCCTCAA TGAAGAAAGA ATTGGACTCT 
CTGAAGGATG AGGAGGAGCT TCCAAAGGTT AAGGAGGTAC TAGCGAAGCC ACAAGATACC 
ACTAGCCCAC TGAATGAGGG ATGTGGTGCC AACCTACCGA GTCCCAAGAG TTTGGGAGAT 
GAGCAGACAC TAGCTTTGCT GGAGCAGTAC TCAGAGCTGT TGCTACAAGC TGTGGAACGA 
CGCATGGACA AAAAACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014805814.1
CDS	220..4740
Translation	MMSVEGSTITSRIKNLLRSPSIKLRRSKPGNKREDIGSKVTLEKVLGITVSGGRGLACDPRTGLVAYPAGCVVVLFNPRKNKQHHILNSSRKTITALAFSPDGKYLVTGESGHMPAVRVWDVAERTQVAELQEHKYGVACVAFSPSSKYIVSVGYQHDMIVNVWSWKKNIVVAANKVSSKVTAVSFSEDCSYFVTAGNRHIKFWYLDDSKTSKVNATVPLLGRSGLLGELRNNFFADVACGRGKKADSTFCITSSGLLCEFNEKRLLDKWVELRNTDSFTTTVANCISVNHDYIFCGCADGTVRIFNPLNLHFVTTLPKPHFLGTDIASVTDASRLFSGMADAKYPDTIALTFDPTNQWLSCVYNDHSLYVWDVKDPKKVGKVYSALYHSSCVWNIEMYPEVKDSNQSCLPPGSFITCSSDNTIRLWNTESSNIHGTALHRNILSNDLMKIIYVDDNTQVLLDTDYNSAGSAEKADAQVMDTKVGIRTVCVSPSGEHLASGDRIGTLRIYELQSLREMLKVEAHDSEILCLEYSKPDTGLKLLASASRDRLIHVLDAGKDYSLQQTLDEHSSSITAVKFAANDGKVRMISCGADKSIYFRTAQKTGEGVQFTRTHHIVRKTTLYDMDVDPSWKYAAIGCQDRNIRVFNISSGKQKKLYKGSQGEDGTLIKVQTDPSGLYIATSCSDKNLSIFDFYSGECVATMYGHSEIVTGMKFSNDCKHLISVSGDSCIFIWRLSSEMTINMRQRLSDMKQRGKQAEKSPPHKTAGLMRHESISALSSVPALSSDSDKDGEDEGNDEDELRGLQSFHLQSNCSTERDSDPDLTLSRSLSHWEMRRAKEIAAIQRSEAPMSKMPRQRGRWSQPTSNIEMTVKSMLDLRQLESFSISRSPSRDSLSQNSNGDDREEQADLPAHINKVGSSVPPQENRSCVRPQVIRLVSCEEGIFSQELEPSESEECVIYPEQDEIHPTDSSRQLSLSSSNLILDSKPIEPPTQTSRLTPDLLKNGSDHPGDALENGQLLESVNSNRTVYVQKKRRSALEASRLGMAPGRVIASFPEGPVNAVMRKAQSVHDLVHEDKGPGVVSSVKQRPQTLLVVEKDPRPNNCRVLSTSMLKMDGSGALLSKDVRAAKSKSYMNPTTSFRAKMSRSVSVGENLYLGYSAEMLTDGRTSPPVAEKTQFSSTADAEKIKPPAKENVPVKPALQPVVNCSSPKSFHSKLASSNRAHLILDIPKPLPDRPTLASFSPTTKPKTLLEPQSPQSPAGACKKKPSFPEVRASKRENQTAGSPVSGKEIPTGLAKESPVESPVSRTGFQDEPGVTNPKLRELSEGRHLRSPEGTLPRCRERITGIACVLDNQPGLCPLDPIRPRSPTSITASAQVSESCISVEQCEHVVSELQDSMRKALHLYRMVLNDTESSTDKDKIAGLLTETFSSMKKELDSLKDEEELPKVKEVLAKPQDTTSPLNEGCGANLPSPKSLGDEQTLALLEQYSELLLQAVERRMDKKL

Target ORF information:

RefSeq Version	XM_014950328.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014950328.1

ORF Insert Sequence:

ATGATGTCGG TCGAAGGGTC TACCATAACA AGTCGGATCA AAAATCTGCT TCGCTCTCCT 
TCCATTAAGC TGAGGAGAAG CAAGCCTGGG AACAAGCGAG AAGATATAGG CAGCAAGGTG 
ACTTTGGAGA AGGTGTTGGG GATAACAGTG TCAGGAGGCA GAGGATTAGC CTGCGACCCC 
AGAACTGGCC TCGTTGCTTA TCCAGCAGGG TGTGTTGTTG TGCTGTTCAA CCCTAGAAAA 
AACAAGCAAC ACCATATTCT AAACAGTTCC AGGAAAACAA TTACAGCACT TGCTTTCTCT 
CCAGATGGAA AATATTTGGT TACAGGAGAG AGTGGACACA TGCCAGCAGT CCGGGTATGG 
GATGTGGCTG AGCGAACCCA GGTGGCTGAG CTCCAGGAGC ACAAGTATGG CGTGGCCTGT 
GTGGCCTTCT CCCCCAGCTC CAAGTACATT GTTTCAGTGG GGTACCAGCA TGACATGATT 
GTCAACGTCT GGTCATGGAA GAAAAACATT GTGGTGGCAG CCAACAAAGT CTCGAGTAAA 
GTGACAGCCG TGTCATTCTC GGAGGACTGC AGTTACTTTG TCACTGCTGG AAATCGACAC 
ATCAAGTTCT GGTACCTGGA TGACAGCAAA ACCTCCAAGG TCAATGCCAC AGTCCCCTTG 
CTGGGCCGCT CAGGATTGCT TGGAGAACTG AGGAACAACT TCTTTGCGGA CGTGGCGTGT 
GGAAGGGGCA AAAAAGCTGA CAGCACCTTC TGTATCACGT CCTCAGGCCT GCTCTGTGAA 
TTCAATGAAA AAAGGCTGCT GGACAAGTGG GTGGAGCTGA GGAATACAGA CAGCTTCACA 
ACCACTGTGG CAAACTGCAT CTCTGTGAAT CATGACTACA TCTTCTGTGG CTGCGCTGAC 
GGCACCGTGA GGATTTTTAA CCCTCTGAAC CTGCACTTTG TCACCACGCT GCCCAAGCCA 
CACTTCCTGG GAACAGACAT CGCGAGTGTG ACCGATGCCA GTCGTCTTTT CTCTGGTATG 
GCTGATGCGA AGTATCCAGA CACGATTGCC TTGACTTTTG ACCCCACCAA CCAGTGGCTT 
TCCTGTGTAT ACAATGACCA CAGCCTGTAC GTGTGGGATG TCAAAGACCC GAAGAAAGTG 
GGCAAGGTGT ACTCCGCTTT GTACCACTCT TCCTGCGTGT GGAACATTGA GATGTATCCA 
GAGGTGAAGG ACAGCAATCA GTCGTGCCTC CCCCCTGGTT CCTTCATCAC GTGCTCCTCT 
GACAACACCA TTCGCCTGTG GAACACGGAG AGCTCCAACA TTCACGGCAC AGCCCTGCAC 
CGCAACATCC TCAGCAATGA CTTGATGAAA ATCATCTATG TAGATGACAA CACTCAGGTA 
CTTCTGGACA CTGATTACAA CTCGGCAGGA AGTGCAGAGA AAGCTGATGC ACAAGTGATG 
GATACGAAGG TGGGGATCCG CACCGTCTGT GTGAGTCCCA GTGGGGAGCA CCTGGCCTCA 
GGGGACAGGA TAGGCACTCT CAGGATATAT GAGTTGCAGT CTCTGAGGGA AATGCTGAAG 
GTGGAAGCCC ATGACTCTGA GATCCTGTGT CTGGAGTACT CCAAACCTGA TACAGGCTTA 
AAGCTCTTAG CCTCAGCCAG TCGGGATAGA TTGATTCATG TCTTGGATGC TGGAAAGGAC 
TACAGCCTGC AGCAGACCCT GGATGAACAT TCTTCTTCTA TCACTGCTGT GAAGTTTGCA 
GCCAATGATG GGAAAGTGAG AATGATAAGC TGTGGGGCAG ACAAGAGCAT CTATTTCCGC 
ACTGCGCAGA AGACAGGAGA AGGGGTCCAG TTTACCCGAA CACATCACAT TGTTAGGAAG 
ACTACTCTGT ATGACATGGA TGTGGACCCC AGCTGGAAAT ATGCAGCTAT TGGATGCCAG 
GATCGTAACA TCAGGGTTTT CAACATAAGC AGTGGGAAGC AGAAGAAGCT TTACAAAGGA 
TCCCAAGGTG AAGATGGTAC CCTCATCAAA GTGCAAACAG ATCCCTCTGG TCTTTATATT 
GCCACCAGTT GTTCGGACAA GAACCTCTCC ATCTTTGATT TCTATTCTGG CGAGTGTGTT 
GCAACCATGT ATGGACATTC AGAGATTGTA ACCGGGATGA AATTCAGTAA TGACTGCAAA 
CATCTGATCT CTGTTTCTGG TGACAGCTGT ATCTTCATCT GGCGTCTGAG CTCGGAGATG 
ACCATCAACA TGCGGCAGCG ACTGTCTGAC ATGAAGCAGA GAGGGAAGCA GGCAGAAAAA 
TCTCCTCCGC ACAAGACAGC TGGACTCATG AGGCACGAGT CCATCTCTGC CCTGTCCTCT 
GTGCCTGCGC TCTCATCAGA CAGTGACAAG GATGGTGAAG ACGAAGGGAA TGATGAAGAT 
GAGCTACGTG GGCTGCAGTC TTTCCATCTT CAGTCCAACT GCAGCACTGA GAGAGACTCA 
GATCCAGACC TTACTCTCTC AAGAAGCCTT TCCCATTGGG AAATGAGGAG GGCCAAAGAG 
ATAGCGGCGA TCCAGCGCTC AGAGGCACCA ATGAGCAAAA TGCCCCGGCA GCGCGGGCGC 
TGGTCGCAGC CCACCAGCAA CATAGAGATG ACGGTGAAAT CCATGCTTGA CTTGCGTCAG 
TTGGAGTCTT TCTCCATTTC CCGTTCTCCC AGTCGAGACT CACTGTCTCA AAACAGCAAC 
GGGGACGACA GAGAAGAGCA GGCAGATCTT CCTGCACACA TCAACAAAGT TGGCAGCTCA 
GTACCTCCCC AAGAGAACCG GTCTTGTGTG CGACCCCAAG TGATTCGCCT TGTGTCTTGT 
GAAGAGGGGA TTTTCTCTCA GGAACTGGAA CCTTCTGAGA GTGAGGAGTG TGTAATCTAC 
CCTGAACAAG ATGAAATCCA TCCCACAGAC TCTAGCAGAC AATTGTCACT CTCTTCATCA 
AATCTGATAC TGGATTCAAA GCCCATTGAG CCGCCAACGC AGACCAGTCG GCTTACACCA 
GACCTGTTGA AAAATGGCAG CGACCATCCT GGTGATGCAT TGGAGAACGG CCAGCTCCTG 
GAGAGTGTAA ATTCAAATCG GACTGTTTAT GTCCAGAAAA AACGCAGATC AGCTTTGGAG 
GCCAGCAGAC TTGGTATGGC TCCTGGACGG GTCATCGCAT CCTTTCCAGA AGGCCCTGTG 
AATGCAGTGA TGAGAAAGGC ACAGTCAGTT CACGACCTCG TTCATGAGGA TAAGGGTCCT 
GGAGTGGTCT CCTCTGTCAA GCAGCGTCCT CAGACTCTTT TGGTGGTTGA GAAGGATCCC 
AGACCTAACA ATTGCAGGGT GTTATCAACC AGTATGTTGA AGATGGATGG ATCTGGTGCT 
CTGCTGTCCA AAGATGTCAG GGCTGCAAAA TCAAAGTCCT ACATGAACCC CACAACCAGC 
TTCCGGGCTA AGATGTCAAG GAGCGTATCT GTAGGGGAAA ATCTGTACCT CGGTTACTCC 
GCCGAAATGT TGACTGATGG GAGGACTAGC CCTCCAGTGG CAGAGAAGAC ACAGTTTAGT 
TCTACAGCAG ATGCTGAGAA GATTAAGCCA CCAGCAAAAG AAAATGTTCC AGTGAAGCCA 
GCACTTCAAC CAGTTGTAAA CTGCTCATCT CCCAAGTCCT TCCACAGCAA GTTGGCATCG 
TCAAACCGAG CACATCTGAT TCTTGACATT CCTAAACCAT TGCCTGATAG ACCCACACTG 
GCCTCCTTCT CACCCACTAC CAAACCAAAA ACCCTACTTG AGCCACAGTC TCCACAGTCA 
CCTGCTGGGG CCTGCAAAAA GAAACCCTCT TTTCCTGAGG TCCGAGCCTC CAAGAGGGAG 
AATCAAACTG CTGGCTCTCC GGTTTCTGGT AAAGAGATCC CAACAGGACT TGCTAAGGAG 
AGCCCAGTGG AAAGTCCGGT CTCAAGGACA GGTTTCCAGG ATGAGCCAGG GGTCACTAAT 
CCAAAACTGA GGGAGTTGTC AGAGGGCCGG CACCTAAGGT CTCCTGAAGG TACACTGCCC 
AGGTGCAGGG AGAGGATCAC CGGCATCGCC TGTGTATTAG ACAACCAACC TGGACTTTGC 
CCACTAGACC CCATCAGGCC GAGGTCTCCT ACATCTATAA CGGCCTCGGC ACAGGTCTCA 
GAATCGTGCA TCAGCGTGGA GCAGTGTGAG CACGTGGTGT CCGAGCTCCA GGACAGCATG 
CGCAAAGCCC TTCATCTGTA CCGCATGGTG TTAAATGATA CGGAGTCTTC TACTGACAAA 
GATAAAATAG CGGGCCTCCT GACAGAAACA TTCTCCTCAA TGAAGAAAGA ATTGGACTCT 
CTGAAGGATG AGGAGGAGCT TCCAAAGGTT AAGGAGGTAC TAGCGAAGCC ACAAGATACC 
ACTAGCCCAC TGAATGAGGG ATGTGGTGCC AACCTACCGA GTCCCAAGAG TTTGGGAGAT 
GAGCAGACAC TAGCTTTGCT GGAGCAGTAC TCAGAGCTGT TGCTACAAGC TGTGGAACGA 
CGCATGGACA AAAAACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MAPKBP1 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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