UBR2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBR2
Entrez Gene ID	106893172
Full Name	ubiquitin protein ligase E3 component n-recognin 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014950910.1	XP_014806396.1	E3 ubiquitin-protein ligase UBR2 isoform X2
XM_014950909.1	XP_014806395.1	E3 ubiquitin-protein ligase UBR2 isoform X1

Pathways from BioSystems
Gene Ontology

The following UBR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa71590	XM_014950909.1 Latest version!	Calidris pugnax ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa71591	XM_014950910.1 Latest version!	Calidris pugnax ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBR2 ( XM_014950909.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014806395.1 Calidris pugnax ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa71590
Clone ID Related Accession (Same CDS sequence)	XM_014950909.1
Accession Version	XM_014950909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090922.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACTTGGAGGC GGAGGTGCAG GCCATTGACA AGAGCCTGCT GGAGTGCTCC 
GCCGAGGAGA TCGCCGTGAA ATGGCTGCAA GCAAATGACC TTCAGAAAGA AGTATACCAG 
CATCTGGCCT ATTATGTACC AAAAATTTAC TGCAGGGGTC CTAACCCTGC CCCACAAAGG 
GAAGACATGG TGGCACAACA CGTCTTGCTG GGACCAATGG AATGGTATCT TTGTGGCGAA 
GATCCTGCAT TTGGTTTTCC AAAGCTTGAA CAAGCAAACA AACCTTCCCA TCTCTGTGGC 
CGTGTTTTTA AAGTGGGAGA GCCTACGTAT TCTTGCAGAG ACTGTGCAGT TGACCCAACT 
TGTGTGTTAT GCATGGAGTG CTTTCTTGGA AGTATTCATA GAGAGCACCG ATACAGGATG 
ACAACATCAG GTGGTGGAGG TTTCTGTGAC TGTGGTGATA CAGAAGCTTG GAAAGAAGGT 
CCTTACTGTC AAAAGCACGA ACTGAACTCT TCAGAGACCG CAGAAGAAGA GGATCCTCTT 
GTGCACTTAC CAGAAGATAT GGTGGCAAGA GCTTATAATA TTTTTGCCAT CGCATTTAAA 
TATGCAGTAG ATATATTAAC ATGGGAAAAG GAAAATGAAT TGCCTGGCCT AGAAATGCCT 
GAGAAGAGTG ACACTTACTA CTGCATGCTG TTTAATGATG AAGTCCATAC CTATGAACAA 
GTTATTTATA CTCTTCAGAA GGCTGTCAAC TGCACGCAGA AGGAAGCTAT TGGTTTTGCA 
ACAACAGTAG ATAGAGATGG ACGAAGGTCC GTTCGATATG GAGACTTTCA GTACTGCGAT 
CAAGCAAAAT CAGTAATAGT GAGAAATACC AGTCGTCAGA CAAAGCCATT GAAAGTACAA 
GTTATGCATT CATCTATCGT TGCCCATCAG AGCTTTGGTC TGAAGCTCCT AACTTGGCTT 
GGCAGTGTTA TTGGATATTC AGATGGTCTT CGCCGGATAT TGTGCCAGGT TGGTTTACAG 
GAGGGGCCAG ATGGTGAAAA TTCTTCTCTT GTGGATAAGT TGATGCTGTA TGATTCTAAA 
CTGTGGAAAG GAGCTAGAAA TGTGTATCAC CAGTTATTCA TGAGCAGTCT ACTTATGGAT 
TTGAAATACA AGAAACTTTT CGCTATCCGC TTCGCAAGAA ACTATGAGCG ATTGCAGAGT 
GATTTTATGA AAGATGACCA CGACAGAGAG TTCTCAATTG CTGACCTCTC GATACAGATA 
TTCACAGTGC CTTCTCTTGC TCGAATGCTT ATAACAGAAG AAAATCTCAT GACCACTATC 
ATCAAAACTT TTATGGACCA CTTACGGCAC CGTGACATCC AAGGCAGGTT TCAGTTTGAA 
CGTTACACTG CTCTGCAGGC TTTCAAATTC AGGCGTGTTC AGAGCCTTAT TTTGGATCTG 
AAGTATGTGT TGATAAGTAA GCCAACTGAG TGGTCAGATG ACTTGAGGCA CAAGTTCCTA 
GAAGGTTTTG ATGCGTTCTT AGAATTACTG AAATGTATGC AGGGTATGGA TCCAATAACT 
CGTCAAGTAG GACAACACAT AGAAATGGAA CCAGAATGGG AGGCAGCATT TACATTGCAG 
ATGAAATTAA CACTTGTTAT TTCAATGATG CAAGACTGGT GTGCATTAGA TGAAAAAGTG 
TTAATTGAAG CTTACAAGAA ATGCCTGACG GTGTTGATGC AGTGTCATAG TGGTTTTACT 
GATGGAGAAC AGCCTATTGT TCTCAGTATG TGTGGACATT CAGTTGAGAC CATCAGATAC 
TGTGTTTCTC AGGAAAAAGT TAGCATTCAT CTCCCAGTTT CTCGTTTACT TGCAGGCTTG 
CATGTTTTGC TGAGTAAAAC TGAAGTGGCA TATAAGTTTC CAGAGCTGCT ACCCCTGAGT 
GAACTTAGCC CACCTATGTT AATCGAACAT CCTCTACGAT GCCTAGTCCT ATGTGCCCAA 
GTGCATGCAG GGATGTGGAG GAGAAATGGG TTCTCTCTTG TTAATCAGAT CTATTACTAT 
CATAATGTGA AGTGCAGGAG GGAGATGTTT GACAAGGACA TAGTAATGCT TCAGACAGGT 
GTGTCTATGA TGGATCCAAA TCACTTTTTG ATGATAATGC TGAGTCGCTT TGAACTTTAT 
CAGATATTTA GTACTCCAGA CTATGGCAAA AGATTCAGCA CAGAAAATAC TAACAAGGAT 
GTTGTTCAAC AAAATAACAC TCTTATAGAA GAGATGCTAT ACCTCATTAT AATGATCATT 
GGTGAAAGAT TCAGTCCTGG AATAGGACAG GTAAACGCAA CAGATGAAAT CAAACGAGAG 
ATCATCCATC AGCTGAGCAT CAGGCCAATG GCTCACAGTG AATTGGTAAA GGCGTTGCCT 
GAAGATGAGA ACAGAGAGAC AGGAATGGAA AGTGTGATTG AAGAAGTGGC ATGTTTCAAG 
AAACCTGGAT TAACAGGCAG AGGACTGTAT GAATTAAAAC CAGAATGTGC CAGAAACTTC 
AATCTATATT TCTATCACTT TTCAAGGGCG GAGCAATCAA AGGCAGAGGA AGCACAAAGA 
AAGCTGAAAA GGCAGAACAG AGAAGATACA GCGCTTCCAC CGCCAGCTCT TCCTCCTTTC 
TGCCCACTTT TTGCAAGTCT GGTTAATATT CTGCAATCTG ATGTCATGCT GTGCATTATG 
GGAACTATAC TGCAGTGGGC TGTAGAACAC AATGGCTATG CCTGGTCGGA GTCCATGCTG 
CAAAGGGTTT TGCATTTGAT TGGAATGGCA CTACAAGAAG AAAAACAGCA TCTGGAGAAT 
CTCAGTGAGG AGAATGTTGT AACATTTACC TTCACTCAGA AAATATCAAA ACCAGGAGAG 
GCACCCAATA ATTCCCCTAG TATACTAGCT ATGCTAGAAA CATTGCAAAA TGCACCTCAT 
CTGGAAGTGC ACAAGGACAT GATCAGATGG ATATTGAAGA CTTTTAATAC TATTAAGAAA 
CTAAGAGAAA GTTCTTCTAC AAGTCCTGTG GCTGAGACAG AAGGAAATAT TATGGAGGAG 
AGTTCACGTG ATAAAGACAA AGCTGAAAGG AAGCGCAAAG CTGAGATTGC CAGACTGCGC 
AGGGAAAAGA TCATGGCCCA GATGTCTGAG ATGCAACGGC ACTTCATTAA TGAAAACAAA 
GAACTCTTTC AGCAAACTTT GGAGGAGCTG GATACTTCAA CCTCTGGAGT ACATGAAAAT 
AGCCCTGTAA TTTCAGATGC AAAACTCACA GCCTTGGGAC CAGAGCAAAC TAGAGTACCT 
GAACACAGAC AGGTTGTTAT GTGTATATTG TGTCAGGAGG AACAAGAAGT TAAAGTAGAC 
AGCAGGGCTA TGGTCTTGGC AGCATTTATT CAGCGATCAA CTGTGTTGTC TAAAAATAGA 
AACAAAATTA TTCCAGACCC TGAAAAGTAT GACCCGTTAT TCATGCACCC AGACCTGTCA 
TGTGGAACGC ACACGGGTAG CTGTGGACAT ATTATGCACG CTCATTGTTG GCAAAGGTAT 
TTTGATGCTG TCCAAGCAAA AGAGCAACGA AGACAGCAAA GATTACGAGT GCACACAAGT 
TATGATGTAG AAAATGGTGA ATTCCTTTGC CCGCTTTGTG AATGCTTAAG CAACACCGTT 
ATTCCTCTGC TTCCTCCACC AAGAGTTTTG TTTAACAGGT TAGACTTTTC AGGCCAACCA 
AACCTATCTC AGTGGATCAA AACAATATCC CAGCAAATAA AAGCGCTGTA TTTACTTCGA 
GATGAAGATG GTGCAAGCAA TTCTGCTAAT TCAGAAAAAA TGGATCAGCT GCAATTACCT 
GAAGGATTTA GACCACATTT CAAACCGAAG AATCCTTATT CTGAGAGCAT AAAAGAGATG 
TTGACAACTT TTGGTACAGC AACATACAAA GTAGGCTTGA AGGTTCATCC AAATGAAGAA 
GATCCACGTG TACCTATAAT GTGCTGGGGA AGCTGCGCTT ACACAATACA GACAATAGAA 
CGAATTCTAG CTGATGAAGA TAAGCCATTA TTTGGTCATT TACCTTGTCG ACAGGATGAC 
TGCCTTACGT CATTAACAAG ATTTGCAGCA GCTCACTGGA CAGTTTCATC TCTTTCGGCA 
GTACAGGGTC ACTTTTGTAC TCTCTTAGCA TCACTGGTGC CTAATGAAAA AAATGGAAAT 
CTTCCTTGCA TACTTGATAT TGACATGTTT CATTTATTGG TAAGCTTGGT GCTCTCTTTC 
CCTGCCATAC ATTGTCAGGA TTTTTCAGGG GTTAGTCTTG GCACTGGAGA TCTTCACCTG 
TTTCATCTTG TCACTATGGC ACACGTGGTA CAAATTTTAC TTACCTCATC AACAGAAGAG 
AATGGCATGG ATCAAGAAAA CACTAACAGT GAAGAGGAAG TAGCTGTGCT TACACTGTAT 
AAATTTCTTT GCCAGTGTAC AGGAAGTGCC TTGAAAGAAA TTTCCTCAGG CTGGCACCTG 
TGGAGGAATC TAAAAGCTGG AATCATGCCT TTCCTGAGAT GTTCTGCTTT GTTTTTCCAT 
TATTTAAATG GAGTCCCAGC ACCCTCAGAA TTGCAAGTAA ATGGAACAAA CCAGTTTGAA 
CACTTATGTA GCTATCTTTC CTTGCCAAAC AACCTCACTT GCCTTTTTCA AGAAAATAGT 
GAGATTCTGA ACACGTTAAT AGAAAGTTGG TGTAGTAACA ATGAAGTAAA AAGATATCTA 
GAAGGCAAGA GACATGCTAT CAGCTATGCA AGAGAATCCA ACAAATTGAT AGACCTTCCG 
GACGACTACA GCTGCCTCAT TAACCAAGCA TCCAATTTCT CGTGTCCAAA ATCAGGTGGT 
GATAAAAGCA GAGCCCCAAC ATTGTGCCTT GTGTGTGGGA CCATGCTGTG TTCTCAGAGT 
TACTGCTGTC AGACTGAGTT AGAAGGAGAA GATGTTGGAG CCTGCACTGC ACATACCTAC 
ACCTGTGGTT CTGGAGTGGG CATATTCCTT CGAGTACGAG AATGTCAGGT GCTATTTTTA 
GCTGGAAAAA CAAAGGGCTG CTTTTATCCT CCTCCTTATC TCGACGATTA CGGAGAGACA 
GACCAGGGAC TCAGACGCGG GAATCCCTTA CATCTGTGCA AAGAACGATT TAAAAAGATT 
CAAAAACTCT GGCAGCAGCA CAGTATCACA GAGGAAATTG GACATGCACA GGAAGCAAAT 
CAAACACTAG TTGGCATTGA TTGGCAGCAT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014806395.1
CDS	53..5308
Translation	MAADLEAEVQAIDKSLLECSAEEIAVKWLQANDLQKEVYQHLAYYVPKIYCRGPNPAPQREDMVAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHREHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNSSETAEEEDPLVHLPEDMVARAYNIFAIAFKYAVDILTWEKENELPGLEMPEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCDQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQSFGLKLLTWLGSVIGYSDGLRRILCQVGLQEGPDGENSSLVDKLMLYDSKLWKGARNVYHQLFMSSLLMDLKYKKLFAIRFARNYERLQSDFMKDDHDREFSIADLSIQIFTVPSLARMLITEENLMTTIIKTFMDHLRHRDIQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRHKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTLVISMMQDWCALDEKVLIEAYKKCLTVLMQCHSGFTDGEQPIVLSMCGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKTEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSTENTNKDVVQQNNTLIEEMLYLIIMIIGERFSPGIGQVNATDEIKREIIHQLSIRPMAHSELVKALPEDENRETGMESVIEEVACFKKPGLTGRGLYELKPECARNFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPALPPFCPLFASLVNILQSDVMLCIMGTILQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLENLSEENVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPHLEVHKDMIRWILKTFNTIKKLRESSSTSPVAETEGNIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFINENKELFQQTLEELDTSTSGVHENSPVISDAKLTALGPEQTRVPEHRQVVMCILCQEEQEVKVDSRAMVLAAFIQRSTVLSKNRNKIIPDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPPPRVLFNRLDFSGQPNLSQWIKTISQQIKALYLLRDEDGASNSANSEKMDQLQLPEGFRPHFKPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQTIERILADEDKPLFGHLPCRQDDCLTSLTRFAAAHWTVSSLSAVQGHFCTLLASLVPNEKNGNLPCILDIDMFHLLVSLVLSFPAIHCQDFSGVSLGTGDLHLFHLVTMAHVVQILLTSSTEENGMDQENTNSEEEVAVLTLYKFLCQCTGSALKEISSGWHLWRNLKAGIMPFLRCSALFFHYLNGVPAPSELQVNGTNQFEHLCSYLSLPNNLTCLFQENSEILNTLIESWCSNNEVKRYLEGKRHAISYARESNKLIDLPDDYSCLINQASNFSCPKSGGDKSRAPTLCLVCGTMLCSQSYCCQTELEGEDVGACTAHTYTCGSGVGIFLRVRECQVLFLAGKTKGCFYPPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_014950909.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014950909.1

ORF Insert Sequence:

ATGGCGGCGG ACTTGGAGGC GGAGGTGCAG GCCATTGACA AGAGCCTGCT GGAGTGCTCC 
GCCGAGGAGA TCGCCGTGAA ATGGCTGCAA GCAAATGACC TTCAGAAAGA AGTATACCAG 
CATCTGGCCT ATTATGTACC AAAAATTTAC TGCAGGGGTC CTAACCCTGC CCCACAAAGG 
GAAGACATGG TGGCACAACA CGTCTTGCTG GGACCAATGG AATGGTATCT TTGTGGCGAA 
GATCCTGCAT TTGGTTTTCC AAAGCTTGAA CAAGCAAACA AACCTTCCCA TCTCTGTGGC 
CGTGTTTTTA AAGTGGGAGA GCCTACGTAT TCTTGCAGAG ACTGTGCAGT TGACCCAACT 
TGTGTGTTAT GCATGGAGTG CTTTCTTGGA AGTATTCATA GAGAGCACCG ATACAGGATG 
ACAACATCAG GTGGTGGAGG TTTCTGTGAC TGTGGTGATA CAGAAGCTTG GAAAGAAGGT 
CCTTACTGTC AAAAGCACGA ACTGAACTCT TCAGAGACCG CAGAAGAAGA GGATCCTCTT 
GTGCACTTAC CAGAAGATAT GGTGGCAAGA GCTTATAATA TTTTTGCCAT CGCATTTAAA 
TATGCAGTAG ATATATTAAC ATGGGAAAAG GAAAATGAAT TGCCTGGCCT AGAAATGCCT 
GAGAAGAGTG ACACTTACTA CTGCATGCTG TTTAATGATG AAGTCCATAC CTATGAACAA 
GTTATTTATA CTCTTCAGAA GGCTGTCAAC TGCACGCAGA AGGAAGCTAT TGGTTTTGCA 
ACAACAGTAG ATAGAGATGG ACGAAGGTCC GTTCGATATG GAGACTTTCA GTACTGCGAT 
CAAGCAAAAT CAGTAATAGT GAGAAATACC AGTCGTCAGA CAAAGCCATT GAAAGTACAA 
GTTATGCATT CATCTATCGT TGCCCATCAG AGCTTTGGTC TGAAGCTCCT AACTTGGCTT 
GGCAGTGTTA TTGGATATTC AGATGGTCTT CGCCGGATAT TGTGCCAGGT TGGTTTACAG 
GAGGGGCCAG ATGGTGAAAA TTCTTCTCTT GTGGATAAGT TGATGCTGTA TGATTCTAAA 
CTGTGGAAAG GAGCTAGAAA TGTGTATCAC CAGTTATTCA TGAGCAGTCT ACTTATGGAT 
TTGAAATACA AGAAACTTTT CGCTATCCGC TTCGCAAGAA ACTATGAGCG ATTGCAGAGT 
GATTTTATGA AAGATGACCA CGACAGAGAG TTCTCAATTG CTGACCTCTC GATACAGATA 
TTCACAGTGC CTTCTCTTGC TCGAATGCTT ATAACAGAAG AAAATCTCAT GACCACTATC 
ATCAAAACTT TTATGGACCA CTTACGGCAC CGTGACATCC AAGGCAGGTT TCAGTTTGAA 
CGTTACACTG CTCTGCAGGC TTTCAAATTC AGGCGTGTTC AGAGCCTTAT TTTGGATCTG 
AAGTATGTGT TGATAAGTAA GCCAACTGAG TGGTCAGATG ACTTGAGGCA CAAGTTCCTA 
GAAGGTTTTG ATGCGTTCTT AGAATTACTG AAATGTATGC AGGGTATGGA TCCAATAACT 
CGTCAAGTAG GACAACACAT AGAAATGGAA CCAGAATGGG AGGCAGCATT TACATTGCAG 
ATGAAATTAA CACTTGTTAT TTCAATGATG CAAGACTGGT GTGCATTAGA TGAAAAAGTG 
TTAATTGAAG CTTACAAGAA ATGCCTGACG GTGTTGATGC AGTGTCATAG TGGTTTTACT 
GATGGAGAAC AGCCTATTGT TCTCAGTATG TGTGGACATT CAGTTGAGAC CATCAGATAC 
TGTGTTTCTC AGGAAAAAGT TAGCATTCAT CTCCCAGTTT CTCGTTTACT TGCAGGCTTG 
CATGTTTTGC TGAGTAAAAC TGAAGTGGCA TATAAGTTTC CAGAGCTGCT ACCCCTGAGT 
GAACTTAGCC CACCTATGTT AATCGAACAT CCTCTACGAT GCCTAGTCCT ATGTGCCCAA 
GTGCATGCAG GGATGTGGAG GAGAAATGGG TTCTCTCTTG TTAATCAGAT CTATTACTAT 
CATAATGTGA AGTGCAGGAG GGAGATGTTT GACAAGGACA TAGTAATGCT TCAGACAGGT 
GTGTCTATGA TGGATCCAAA TCACTTTTTG ATGATAATGC TGAGTCGCTT TGAACTTTAT 
CAGATATTTA GTACTCCAGA CTATGGCAAA AGATTCAGCA CAGAAAATAC TAACAAGGAT 
GTTGTTCAAC AAAATAACAC TCTTATAGAA GAGATGCTAT ACCTCATTAT AATGATCATT 
GGTGAAAGAT TCAGTCCTGG AATAGGACAG GTAAACGCAA CAGATGAAAT CAAACGAGAG 
ATCATCCATC AGCTGAGCAT CAGGCCAATG GCTCACAGTG AATTGGTAAA GGCGTTGCCT 
GAAGATGAGA ACAGAGAGAC AGGAATGGAA AGTGTGATTG AAGAAGTGGC ATGTTTCAAG 
AAACCTGGAT TAACAGGCAG AGGACTGTAT GAATTAAAAC CAGAATGTGC CAGAAACTTC 
AATCTATATT TCTATCACTT TTCAAGGGCG GAGCAATCAA AGGCAGAGGA AGCACAAAGA 
AAGCTGAAAA GGCAGAACAG AGAAGATACA GCGCTTCCAC CGCCAGCTCT TCCTCCTTTC 
TGCCCACTTT TTGCAAGTCT GGTTAATATT CTGCAATCTG ATGTCATGCT GTGCATTATG 
GGAACTATAC TGCAGTGGGC TGTAGAACAC AATGGCTATG CCTGGTCGGA GTCCATGCTG 
CAAAGGGTTT TGCATTTGAT TGGAATGGCA CTACAAGAAG AAAAACAGCA TCTGGAGAAT 
CTCAGTGAGG AGAATGTTGT AACATTTACC TTCACTCAGA AAATATCAAA ACCAGGAGAG 
GCACCCAATA ATTCCCCTAG TATACTAGCT ATGCTAGAAA CATTGCAAAA TGCACCTCAT 
CTGGAAGTGC ACAAGGACAT GATCAGATGG ATATTGAAGA CTTTTAATAC TATTAAGAAA 
CTAAGAGAAA GTTCTTCTAC AAGTCCTGTG GCTGAGACAG AAGGAAATAT TATGGAGGAG 
AGTTCACGTG ATAAAGACAA AGCTGAAAGG AAGCGCAAAG CTGAGATTGC CAGACTGCGC 
AGGGAAAAGA TCATGGCCCA GATGTCTGAG ATGCAACGGC ACTTCATTAA TGAAAACAAA 
GAACTCTTTC AGCAAACTTT GGAGGAGCTG GATACTTCAA CCTCTGGAGT ACATGAAAAT 
AGCCCTGTAA TTTCAGATGC AAAACTCACA GCCTTGGGAC CAGAGCAAAC TAGAGTACCT 
GAACACAGAC AGGTTGTTAT GTGTATATTG TGTCAGGAGG AACAAGAAGT TAAAGTAGAC 
AGCAGGGCTA TGGTCTTGGC AGCATTTATT CAGCGATCAA CTGTGTTGTC TAAAAATAGA 
AACAAAATTA TTCCAGACCC TGAAAAGTAT GACCCGTTAT TCATGCACCC AGACCTGTCA 
TGTGGAACGC ACACGGGTAG CTGTGGACAT ATTATGCACG CTCATTGTTG GCAAAGGTAT 
TTTGATGCTG TCCAAGCAAA AGAGCAACGA AGACAGCAAA GATTACGAGT GCACACAAGT 
TATGATGTAG AAAATGGTGA ATTCCTTTGC CCGCTTTGTG AATGCTTAAG CAACACCGTT 
ATTCCTCTGC TTCCTCCACC AAGAGTTTTG TTTAACAGGT TAGACTTTTC AGGCCAACCA 
AACCTATCTC AGTGGATCAA AACAATATCC CAGCAAATAA AAGCGCTGTA TTTACTTCGA 
GATGAAGATG GTGCAAGCAA TTCTGCTAAT TCAGAAAAAA TGGATCAGCT GCAATTACCT 
GAAGGATTTA GACCACATTT CAAACCGAAG AATCCTTATT CTGAGAGCAT AAAAGAGATG 
TTGACAACTT TTGGTACAGC AACATACAAA GTAGGCTTGA AGGTTCATCC AAATGAAGAA 
GATCCACGTG TACCTATAAT GTGCTGGGGA AGCTGCGCTT ACACAATACA GACAATAGAA 
CGAATTCTAG CTGATGAAGA TAAGCCATTA TTTGGTCATT TACCTTGTCG ACAGGATGAC 
TGCCTTACGT CATTAACAAG ATTTGCAGCA GCTCACTGGA CAGTTTCATC TCTTTCGGCA 
GTACAGGGTC ACTTTTGTAC TCTCTTAGCA TCACTGGTGC CTAATGAAAA AAATGGAAAT 
CTTCCTTGCA TACTTGATAT TGACATGTTT CATTTATTGG TAAGCTTGGT GCTCTCTTTC 
CCTGCCATAC ATTGTCAGGA TTTTTCAGGG GTTAGTCTTG GCACTGGAGA TCTTCACCTG 
TTTCATCTTG TCACTATGGC ACACGTGGTA CAAATTTTAC TTACCTCATC AACAGAAGAG 
AATGGCATGG ATCAAGAAAA CACTAACAGT GAAGAGGAAG TAGCTGTGCT TACACTGTAT 
AAATTTCTTT GCCAGTGTAC AGGAAGTGCC TTGAAAGAAA TTTCCTCAGG CTGGCACCTG 
TGGAGGAATC TAAAAGCTGG AATCATGCCT TTCCTGAGAT GTTCTGCTTT GTTTTTCCAT 
TATTTAAATG GAGTCCCAGC ACCCTCAGAA TTGCAAGTAA ATGGAACAAA CCAGTTTGAA 
CACTTATGTA GCTATCTTTC CTTGCCAAAC AACCTCACTT GCCTTTTTCA AGAAAATAGT 
GAGATTCTGA ACACGTTAAT AGAAAGTTGG TGTAGTAACA ATGAAGTAAA AAGATATCTA 
GAAGGCAAGA GACATGCTAT CAGCTATGCA AGAGAATCCA ACAAATTGAT AGACCTTCCG 
GACGACTACA GCTGCCTCAT TAACCAAGCA TCCAATTTCT CGTGTCCAAA ATCAGGTGGT 
GATAAAAGCA GAGCCCCAAC ATTGTGCCTT GTGTGTGGGA CCATGCTGTG TTCTCAGAGT 
TACTGCTGTC AGACTGAGTT AGAAGGAGAA GATGTTGGAG CCTGCACTGC ACATACCTAC 
ACCTGTGGTT CTGGAGTGGG CATATTCCTT CGAGTACGAG AATGTCAGGT GCTATTTTTA 
GCTGGAAAAA CAAAGGGCTG CTTTTATCCT CCTCCTTATC TCGACGATTA CGGAGAGACA 
GACCAGGGAC TCAGACGCGG GAATCCCTTA CATCTGTGCA AAGAACGATT TAAAAAGATT 
CAAAAACTCT GGCAGCAGCA CAGTATCACA GAGGAAATTG GACATGCACA GGAAGCAAAT 
CAAACACTAG TTGGCATTGA TTGGCAGCAT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_014950910.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014806396.1 Calidris pugnax ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa71591
Clone ID Related Accession (Same CDS sequence)	XM_014950910.1
Accession Version	XM_014950910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090922.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACTTGGAGGC GGAGGTGCAG GCCATTGACA AGAGCCTGCT GGAGTGCTCC 
GCCGAGGAGA TCGCCGTGAA ATGGCTGCAA GCAAATGACC TTCAGAAAGA AGTATACCAG 
CATCTGGCCT ATTATGTACC AAAAATTTAC TGCAGGGGTC CTAACCCTGC CCCACAAAGG 
GAAGACATGG TGGCACAACA CGTCTTGCTG GGACCAATGG AATGGTATCT TTGTGGCGAA 
GATCCTGCAT TTGGTTTTCC AAAGCTTGAA CAAGCAAACA AACCTTCCCA TCTCTGTGGC 
CGTGTTTTTA AAGTGGGAGA GCCTACGTAT TCTTGCAGAG ACTGTGCAGT TGACCCAACT 
TGTGTGTTAT GCATGGAGTG CTTTCTTGGA AGTATTCATA GAGAGCACCG ATACAGGATG 
ACAACATCAG GTGGTGGAGG TTTCTGTGAC TGTGGTGATA CAGAAGCTTG GAAAGAAGGT 
CCTTACTGTC AAAAGCACGA ACTGAACTCT TCAGAGACCG CAGAAGAAGA GGATCCTCTT 
GTGCACTTAC CAGAAGATAT GGTGGCAAGA GCTTATAATA TTTTTGCCAT CGCATTTAAA 
TATGCAGTAG ATATATTAAC ATGGGAAAAG GAAAATGAAT TGCCTGGCCT AGAAATGCCT 
GAGAAGAGTG ACACTTACTA CTGCATGCTG TTTAATGATG AAGTCCATAC CTATGAACAA 
GTTATTTATA CTCTTCAGAA GGCTGTCAAC TGCACGCAGA AGGAAGCTAT TGGTTTTGCA 
ACAACAGTAG ATAGAGATGG ACGAAGGTCC GTTCGATATG GAGACTTTCA GTACTGCGAT 
CAAGCAAAAT CAGTAATAGT GAGAAATACC AGTCGTCAGA CAAAGCCATT GAAAGTACAA 
GTTATGCATT CATCTATCGT TGCCCATCAG AGCTTTGGTC TGAAGCTCCT AACTTGGCTT 
GGCAGTGTTA TTGGATATTC AGATGGTCTT CGCCGGATAT TGTGCCAGGT TGGTTTACAG 
GAGGGGCCAG ATGGTGAAAA TTCTTCTCTT GTGGATAAGT TGATGCTGTA TGATTCTAAA 
CTGTGGAAAG GAGCTAGAAA TGTGTATCAC CAGTTATTCA TGAGCAGTCT ACTTATGGAT 
TTGAAATACA AGAAACTTTT CGCTATCCGC TTCGCAAGAA ATTACCGGCA GTTGCAGAGA 
GATTATATGG AGGATGATCA CGAACGGGCA GTATCGGTGG CTGCTCTGTC TGTCCAACTC 
TTCACTGTAC CTACTCTGGC TCGAATGCTT ATAACAGAAG AAAATCTCAT GACCACTATC 
ATCAAAACTT TTATGGACCA CTTACGGCAC CGTGACATCC AAGGCAGGTT TCAGTTTGAA 
CGTTACACTG CTCTGCAGGC TTTCAAATTC AGGCGTGTTC AGAGCCTTAT TTTGGATCTG 
AAGTATGTGT TGATAAGTAA GCCAACTGAG TGGTCAGATG ACTTGAGGCA CAAGTTCCTA 
GAAGGTTTTG ATGCGTTCTT AGAATTACTG AAATGTATGC AGGGTATGGA TCCAATAACT 
CGTCAAGTAG GACAACACAT AGAAATGGAA CCAGAATGGG AGGCAGCATT TACATTGCAG 
ATGAAATTAA CACTTGTTAT TTCAATGATG CAAGACTGGT GTGCATTAGA TGAAAAAGTG 
TTAATTGAAG CTTACAAGAA ATGCCTGACG GTGTTGATGC AGTGTCATAG TGGTTTTACT 
GATGGAGAAC AGCCTATTGT TCTCAGTATG TGTGGACATT CAGTTGAGAC CATCAGATAC 
TGTGTTTCTC AGGAAAAAGT TAGCATTCAT CTCCCAGTTT CTCGTTTACT TGCAGGCTTG 
CATGTTTTGC TGAGTAAAAC TGAAGTGGCA TATAAGTTTC CAGAGCTGCT ACCCCTGAGT 
GAACTTAGCC CACCTATGTT AATCGAACAT CCTCTACGAT GCCTAGTCCT ATGTGCCCAA 
GTGCATGCAG GGATGTGGAG GAGAAATGGG TTCTCTCTTG TTAATCAGAT CTATTACTAT 
CATAATGTGA AGTGCAGGAG GGAGATGTTT GACAAGGACA TAGTAATGCT TCAGACAGGT 
GTGTCTATGA TGGATCCAAA TCACTTTTTG ATGATAATGC TGAGTCGCTT TGAACTTTAT 
CAGATATTTA GTACTCCAGA CTATGGCAAA AGATTCAGCA CAGAAAATAC TAACAAGGAT 
GTTGTTCAAC AAAATAACAC TCTTATAGAA GAGATGCTAT ACCTCATTAT AATGATCATT 
GGTGAAAGAT TCAGTCCTGG AATAGGACAG GTAAACGCAA CAGATGAAAT CAAACGAGAG 
ATCATCCATC AGCTGAGCAT CAGGCCAATG GCTCACAGTG AATTGGTAAA GGCGTTGCCT 
GAAGATGAGA ACAGAGAGAC AGGAATGGAA AGTGTGATTG AAGAAGTGGC ATGTTTCAAG 
AAACCTGGAT TAACAGGCAG AGGACTGTAT GAATTAAAAC CAGAATGTGC CAGAAACTTC 
AATCTATATT TCTATCACTT TTCAAGGGCG GAGCAATCAA AGGCAGAGGA AGCACAAAGA 
AAGCTGAAAA GGCAGAACAG AGAAGATACA GCGCTTCCAC CGCCAGCTCT TCCTCCTTTC 
TGCCCACTTT TTGCAAGTCT GGTTAATATT CTGCAATCTG ATGTCATGCT GTGCATTATG 
GGAACTATAC TGCAGTGGGC TGTAGAACAC AATGGCTATG CCTGGTCGGA GTCCATGCTG 
CAAAGGGTTT TGCATTTGAT TGGAATGGCA CTACAAGAAG AAAAACAGCA TCTGGAGAAT 
CTCAGTGAGG AGAATGTTGT AACATTTACC TTCACTCAGA AAATATCAAA ACCAGGAGAG 
GCACCCAATA ATTCCCCTAG TATACTAGCT ATGCTAGAAA CATTGCAAAA TGCACCTCAT 
CTGGAAGTGC ACAAGGACAT GATCAGATGG ATATTGAAGA CTTTTAATAC TATTAAGAAA 
CTAAGAGAAA GTTCTTCTAC AAGTCCTGTG GCTGAGACAG AAGGAAATAT TATGGAGGAG 
AGTTCACGTG ATAAAGACAA AGCTGAAAGG AAGCGCAAAG CTGAGATTGC CAGACTGCGC 
AGGGAAAAGA TCATGGCCCA GATGTCTGAG ATGCAACGGC ACTTCATTAA TGAAAACAAA 
GAACTCTTTC AGCAAACTTT GGAGGAGCTG GATACTTCAA CCTCTGGAGT ACATGAAAAT 
AGCCCTGTAA TTTCAGATGC AAAACTCACA GCCTTGGGAC CAGAGCAAAC TAGAGTACCT 
GAACACAGAC AGGTTGTTAT GTGTATATTG TGTCAGGAGG AACAAGAAGT TAAAGTAGAC 
AGCAGGGCTA TGGTCTTGGC AGCATTTATT CAGCGATCAA CTGTGTTGTC TAAAAATAGA 
AACAAAATTA TTCCAGACCC TGAAAAGTAT GACCCGTTAT TCATGCACCC AGACCTGTCA 
TGTGGAACGC ACACGGGTAG CTGTGGACAT ATTATGCACG CTCATTGTTG GCAAAGGTAT 
TTTGATGCTG TCCAAGCAAA AGAGCAACGA AGACAGCAAA GATTACGAGT GCACACAAGT 
TATGATGTAG AAAATGGTGA ATTCCTTTGC CCGCTTTGTG AATGCTTAAG CAACACCGTT 
ATTCCTCTGC TTCCTCCACC AAGAGTTTTG TTTAACAGGT TAGACTTTTC AGGCCAACCA 
AACCTATCTC AGTGGATCAA AACAATATCC CAGCAAATAA AAGCGCTGTA TTTACTTCGA 
GATGAAGATG GTGCAAGCAA TTCTGCTAAT TCAGAAAAAA TGGATCAGCT GCAATTACCT 
GAAGGATTTA GACCACATTT CAAACCGAAG AATCCTTATT CTGAGAGCAT AAAAGAGATG 
TTGACAACTT TTGGTACAGC AACATACAAA GTAGGCTTGA AGGTTCATCC AAATGAAGAA 
GATCCACGTG TACCTATAAT GTGCTGGGGA AGCTGCGCTT ACACAATACA GACAATAGAA 
CGAATTCTAG CTGATGAAGA TAAGCCATTA TTTGGTCATT TACCTTGTCG ACAGGATGAC 
TGCCTTACGT CATTAACAAG ATTTGCAGCA GCTCACTGGA CAGTTTCATC TCTTTCGGCA 
GTACAGGGTC ACTTTTGTAC TCTCTTAGCA TCACTGGTGC CTAATGAAAA AAATGGAAAT 
CTTCCTTGCA TACTTGATAT TGACATGTTT CATTTATTGG TAAGCTTGGT GCTCTCTTTC 
CCTGCCATAC ATTGTCAGGA TTTTTCAGGG GTTAGTCTTG GCACTGGAGA TCTTCACCTG 
TTTCATCTTG TCACTATGGC ACACGTGGTA CAAATTTTAC TTACCTCATC AACAGAAGAG 
AATGGCATGG ATCAAGAAAA CACTAACAGT GAAGAGGAAG TAGCTGTGCT TACACTGTAT 
AAATTTCTTT GCCAGTGTAC AGGAAGTGCC TTGAAAGAAA TTTCCTCAGG CTGGCACCTG 
TGGAGGAATC TAAAAGCTGG AATCATGCCT TTCCTGAGAT GTTCTGCTTT GTTTTTCCAT 
TATTTAAATG GAGTCCCAGC ACCCTCAGAA TTGCAAGTAA ATGGAACAAA CCAGTTTGAA 
CACTTATGTA GCTATCTTTC CTTGCCAAAC AACCTCACTT GCCTTTTTCA AGAAAATAGT 
GAGATTCTGA ACACGTTAAT AGAAAGTTGG TGTAGTAACA ATGAAGTAAA AAGATATCTA 
GAAGGCAAGA GACATGCTAT CAGCTATGCA AGAGAATCCA ACAAATTGAT AGACCTTCCG 
GACGACTACA GCTGCCTCAT TAACCAAGCA TCCAATTTCT CGTGTCCAAA ATCAGGTGGT 
GATAAAAGCA GAGCCCCAAC ATTGTGCCTT GTGTGTGGGA CCATGCTGTG TTCTCAGAGT 
TACTGCTGTC AGACTGAGTT AGAAGGAGAA GATGTTGGAG CCTGCACTGC ACATACCTAC 
ACCTGTGGTT CTGGAGTGGG CATATTCCTT CGAGTACGAG AATGTCAGGT GCTATTTTTA 
GCTGGAAAAA CAAAGGGCTG CTTTTATCCT CCTCCTTATC TCGACGATTA CGGAGAGACA 
GACCAGGGAC TCAGACGCGG GAATCCCTTA CATCTGTGCA AAGAACGATT TAAAAAGATT 
CAAAAACTCT GGCAGCAGCA CAGTATCACA GAGGAAATTG GACATGCACA GGAAGCAAAT 
CAAACACTAG TTGGCATTGA TTGGCAGCAT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014806396.1
CDS	53..5308
Translation	MAADLEAEVQAIDKSLLECSAEEIAVKWLQANDLQKEVYQHLAYYVPKIYCRGPNPAPQREDMVAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHREHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNSSETAEEEDPLVHLPEDMVARAYNIFAIAFKYAVDILTWEKENELPGLEMPEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCDQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQSFGLKLLTWLGSVIGYSDGLRRILCQVGLQEGPDGENSSLVDKLMLYDSKLWKGARNVYHQLFMSSLLMDLKYKKLFAIRFARNYRQLQRDYMEDDHERAVSVAALSVQLFTVPTLARMLITEENLMTTIIKTFMDHLRHRDIQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDDLRHKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTLVISMMQDWCALDEKVLIEAYKKCLTVLMQCHSGFTDGEQPIVLSMCGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKTEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSTENTNKDVVQQNNTLIEEMLYLIIMIIGERFSPGIGQVNATDEIKREIIHQLSIRPMAHSELVKALPEDENRETGMESVIEEVACFKKPGLTGRGLYELKPECARNFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPALPPFCPLFASLVNILQSDVMLCIMGTILQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLENLSEENVVTFTFTQKISKPGEAPNNSPSILAMLETLQNAPHLEVHKDMIRWILKTFNTIKKLRESSSTSPVAETEGNIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFINENKELFQQTLEELDTSTSGVHENSPVISDAKLTALGPEQTRVPEHRQVVMCILCQEEQEVKVDSRAMVLAAFIQRSTVLSKNRNKIIPDPEKYDPLFMHPDLSCGTHTGSCGHIMHAHCWQRYFDAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPPPRVLFNRLDFSGQPNLSQWIKTISQQIKALYLLRDEDGASNSANSEKMDQLQLPEGFRPHFKPKNPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQTIERILADEDKPLFGHLPCRQDDCLTSLTRFAAAHWTVSSLSAVQGHFCTLLASLVPNEKNGNLPCILDIDMFHLLVSLVLSFPAIHCQDFSGVSLGTGDLHLFHLVTMAHVVQILLTSSTEENGMDQENTNSEEEVAVLTLYKFLCQCTGSALKEISSGWHLWRNLKAGIMPFLRCSALFFHYLNGVPAPSELQVNGTNQFEHLCSYLSLPNNLTCLFQENSEILNTLIESWCSNNEVKRYLEGKRHAISYARESNKLIDLPDDYSCLINQASNFSCPKSGGDKSRAPTLCLVCGTMLCSQSYCCQTELEGEDVGACTAHTYTCGSGVGIFLRVRECQVLFLAGKTKGCFYPPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_014950910.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014950910.1

ORF Insert Sequence:

ATGGCGGCGG ACTTGGAGGC GGAGGTGCAG GCCATTGACA AGAGCCTGCT GGAGTGCTCC 
GCCGAGGAGA TCGCCGTGAA ATGGCTGCAA GCAAATGACC TTCAGAAAGA AGTATACCAG 
CATCTGGCCT ATTATGTACC AAAAATTTAC TGCAGGGGTC CTAACCCTGC CCCACAAAGG 
GAAGACATGG TGGCACAACA CGTCTTGCTG GGACCAATGG AATGGTATCT TTGTGGCGAA 
GATCCTGCAT TTGGTTTTCC AAAGCTTGAA CAAGCAAACA AACCTTCCCA TCTCTGTGGC 
CGTGTTTTTA AAGTGGGAGA GCCTACGTAT TCTTGCAGAG ACTGTGCAGT TGACCCAACT 
TGTGTGTTAT GCATGGAGTG CTTTCTTGGA AGTATTCATA GAGAGCACCG ATACAGGATG 
ACAACATCAG GTGGTGGAGG TTTCTGTGAC TGTGGTGATA CAGAAGCTTG GAAAGAAGGT 
CCTTACTGTC AAAAGCACGA ACTGAACTCT TCAGAGACCG CAGAAGAAGA GGATCCTCTT 
GTGCACTTAC CAGAAGATAT GGTGGCAAGA GCTTATAATA TTTTTGCCAT CGCATTTAAA 
TATGCAGTAG ATATATTAAC ATGGGAAAAG GAAAATGAAT TGCCTGGCCT AGAAATGCCT 
GAGAAGAGTG ACACTTACTA CTGCATGCTG TTTAATGATG AAGTCCATAC CTATGAACAA 
GTTATTTATA CTCTTCAGAA GGCTGTCAAC TGCACGCAGA AGGAAGCTAT TGGTTTTGCA 
ACAACAGTAG ATAGAGATGG ACGAAGGTCC GTTCGATATG GAGACTTTCA GTACTGCGAT 
CAAGCAAAAT CAGTAATAGT GAGAAATACC AGTCGTCAGA CAAAGCCATT GAAAGTACAA 
GTTATGCATT CATCTATCGT TGCCCATCAG AGCTTTGGTC TGAAGCTCCT AACTTGGCTT 
GGCAGTGTTA TTGGATATTC AGATGGTCTT CGCCGGATAT TGTGCCAGGT TGGTTTACAG 
GAGGGGCCAG ATGGTGAAAA TTCTTCTCTT GTGGATAAGT TGATGCTGTA TGATTCTAAA 
CTGTGGAAAG GAGCTAGAAA TGTGTATCAC CAGTTATTCA TGAGCAGTCT ACTTATGGAT 
TTGAAATACA AGAAACTTTT CGCTATCCGC TTCGCAAGAA ATTACCGGCA GTTGCAGAGA 
GATTATATGG AGGATGATCA CGAACGGGCA GTATCGGTGG CTGCTCTGTC TGTCCAACTC 
TTCACTGTAC CTACTCTGGC TCGAATGCTT ATAACAGAAG AAAATCTCAT GACCACTATC 
ATCAAAACTT TTATGGACCA CTTACGGCAC CGTGACATCC AAGGCAGGTT TCAGTTTGAA 
CGTTACACTG CTCTGCAGGC TTTCAAATTC AGGCGTGTTC AGAGCCTTAT TTTGGATCTG 
AAGTATGTGT TGATAAGTAA GCCAACTGAG TGGTCAGATG ACTTGAGGCA CAAGTTCCTA 
GAAGGTTTTG ATGCGTTCTT AGAATTACTG AAATGTATGC AGGGTATGGA TCCAATAACT 
CGTCAAGTAG GACAACACAT AGAAATGGAA CCAGAATGGG AGGCAGCATT TACATTGCAG 
ATGAAATTAA CACTTGTTAT TTCAATGATG CAAGACTGGT GTGCATTAGA TGAAAAAGTG 
TTAATTGAAG CTTACAAGAA ATGCCTGACG GTGTTGATGC AGTGTCATAG TGGTTTTACT 
GATGGAGAAC AGCCTATTGT TCTCAGTATG TGTGGACATT CAGTTGAGAC CATCAGATAC 
TGTGTTTCTC AGGAAAAAGT TAGCATTCAT CTCCCAGTTT CTCGTTTACT TGCAGGCTTG 
CATGTTTTGC TGAGTAAAAC TGAAGTGGCA TATAAGTTTC CAGAGCTGCT ACCCCTGAGT 
GAACTTAGCC CACCTATGTT AATCGAACAT CCTCTACGAT GCCTAGTCCT ATGTGCCCAA 
GTGCATGCAG GGATGTGGAG GAGAAATGGG TTCTCTCTTG TTAATCAGAT CTATTACTAT 
CATAATGTGA AGTGCAGGAG GGAGATGTTT GACAAGGACA TAGTAATGCT TCAGACAGGT 
GTGTCTATGA TGGATCCAAA TCACTTTTTG ATGATAATGC TGAGTCGCTT TGAACTTTAT 
CAGATATTTA GTACTCCAGA CTATGGCAAA AGATTCAGCA CAGAAAATAC TAACAAGGAT 
GTTGTTCAAC AAAATAACAC TCTTATAGAA GAGATGCTAT ACCTCATTAT AATGATCATT 
GGTGAAAGAT TCAGTCCTGG AATAGGACAG GTAAACGCAA CAGATGAAAT CAAACGAGAG 
ATCATCCATC AGCTGAGCAT CAGGCCAATG GCTCACAGTG AATTGGTAAA GGCGTTGCCT 
GAAGATGAGA ACAGAGAGAC AGGAATGGAA AGTGTGATTG AAGAAGTGGC ATGTTTCAAG 
AAACCTGGAT TAACAGGCAG AGGACTGTAT GAATTAAAAC CAGAATGTGC CAGAAACTTC 
AATCTATATT TCTATCACTT TTCAAGGGCG GAGCAATCAA AGGCAGAGGA AGCACAAAGA 
AAGCTGAAAA GGCAGAACAG AGAAGATACA GCGCTTCCAC CGCCAGCTCT TCCTCCTTTC 
TGCCCACTTT TTGCAAGTCT GGTTAATATT CTGCAATCTG ATGTCATGCT GTGCATTATG 
GGAACTATAC TGCAGTGGGC TGTAGAACAC AATGGCTATG CCTGGTCGGA GTCCATGCTG 
CAAAGGGTTT TGCATTTGAT TGGAATGGCA CTACAAGAAG AAAAACAGCA TCTGGAGAAT 
CTCAGTGAGG AGAATGTTGT AACATTTACC TTCACTCAGA AAATATCAAA ACCAGGAGAG 
GCACCCAATA ATTCCCCTAG TATACTAGCT ATGCTAGAAA CATTGCAAAA TGCACCTCAT 
CTGGAAGTGC ACAAGGACAT GATCAGATGG ATATTGAAGA CTTTTAATAC TATTAAGAAA 
CTAAGAGAAA GTTCTTCTAC AAGTCCTGTG GCTGAGACAG AAGGAAATAT TATGGAGGAG 
AGTTCACGTG ATAAAGACAA AGCTGAAAGG AAGCGCAAAG CTGAGATTGC CAGACTGCGC 
AGGGAAAAGA TCATGGCCCA GATGTCTGAG ATGCAACGGC ACTTCATTAA TGAAAACAAA 
GAACTCTTTC AGCAAACTTT GGAGGAGCTG GATACTTCAA CCTCTGGAGT ACATGAAAAT 
AGCCCTGTAA TTTCAGATGC AAAACTCACA GCCTTGGGAC CAGAGCAAAC TAGAGTACCT 
GAACACAGAC AGGTTGTTAT GTGTATATTG TGTCAGGAGG AACAAGAAGT TAAAGTAGAC 
AGCAGGGCTA TGGTCTTGGC AGCATTTATT CAGCGATCAA CTGTGTTGTC TAAAAATAGA 
AACAAAATTA TTCCAGACCC TGAAAAGTAT GACCCGTTAT TCATGCACCC AGACCTGTCA 
TGTGGAACGC ACACGGGTAG CTGTGGACAT ATTATGCACG CTCATTGTTG GCAAAGGTAT 
TTTGATGCTG TCCAAGCAAA AGAGCAACGA AGACAGCAAA GATTACGAGT GCACACAAGT 
TATGATGTAG AAAATGGTGA ATTCCTTTGC CCGCTTTGTG AATGCTTAAG CAACACCGTT 
ATTCCTCTGC TTCCTCCACC AAGAGTTTTG TTTAACAGGT TAGACTTTTC AGGCCAACCA 
AACCTATCTC AGTGGATCAA AACAATATCC CAGCAAATAA AAGCGCTGTA TTTACTTCGA 
GATGAAGATG GTGCAAGCAA TTCTGCTAAT TCAGAAAAAA TGGATCAGCT GCAATTACCT 
GAAGGATTTA GACCACATTT CAAACCGAAG AATCCTTATT CTGAGAGCAT AAAAGAGATG 
TTGACAACTT TTGGTACAGC AACATACAAA GTAGGCTTGA AGGTTCATCC AAATGAAGAA 
GATCCACGTG TACCTATAAT GTGCTGGGGA AGCTGCGCTT ACACAATACA GACAATAGAA 
CGAATTCTAG CTGATGAAGA TAAGCCATTA TTTGGTCATT TACCTTGTCG ACAGGATGAC 
TGCCTTACGT CATTAACAAG ATTTGCAGCA GCTCACTGGA CAGTTTCATC TCTTTCGGCA 
GTACAGGGTC ACTTTTGTAC TCTCTTAGCA TCACTGGTGC CTAATGAAAA AAATGGAAAT 
CTTCCTTGCA TACTTGATAT TGACATGTTT CATTTATTGG TAAGCTTGGT GCTCTCTTTC 
CCTGCCATAC ATTGTCAGGA TTTTTCAGGG GTTAGTCTTG GCACTGGAGA TCTTCACCTG 
TTTCATCTTG TCACTATGGC ACACGTGGTA CAAATTTTAC TTACCTCATC AACAGAAGAG 
AATGGCATGG ATCAAGAAAA CACTAACAGT GAAGAGGAAG TAGCTGTGCT TACACTGTAT 
AAATTTCTTT GCCAGTGTAC AGGAAGTGCC TTGAAAGAAA TTTCCTCAGG CTGGCACCTG 
TGGAGGAATC TAAAAGCTGG AATCATGCCT TTCCTGAGAT GTTCTGCTTT GTTTTTCCAT 
TATTTAAATG GAGTCCCAGC ACCCTCAGAA TTGCAAGTAA ATGGAACAAA CCAGTTTGAA 
CACTTATGTA GCTATCTTTC CTTGCCAAAC AACCTCACTT GCCTTTTTCA AGAAAATAGT 
GAGATTCTGA ACACGTTAAT AGAAAGTTGG TGTAGTAACA ATGAAGTAAA AAGATATCTA 
GAAGGCAAGA GACATGCTAT CAGCTATGCA AGAGAATCCA ACAAATTGAT AGACCTTCCG 
GACGACTACA GCTGCCTCAT TAACCAAGCA TCCAATTTCT CGTGTCCAAA ATCAGGTGGT 
GATAAAAGCA GAGCCCCAAC ATTGTGCCTT GTGTGTGGGA CCATGCTGTG TTCTCAGAGT 
TACTGCTGTC AGACTGAGTT AGAAGGAGAA GATGTTGGAG CCTGCACTGC ACATACCTAC 
ACCTGTGGTT CTGGAGTGGG CATATTCCTT CGAGTACGAG AATGTCAGGT GCTATTTTTA 
GCTGGAAAAA CAAAGGGCTG CTTTTATCCT CCTCCTTATC TCGACGATTA CGGAGAGACA 
GACCAGGGAC TCAGACGCGG GAATCCCTTA CATCTGTGCA AAGAACGATT TAAAAAGATT 
CAAAAACTCT GGCAGCAGCA CAGTATCACA GAGGAAATTG GACATGCACA GGAAGCAAAT 
CAAACACTAG TTGGCATTGA TTGGCAGCAT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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UBR2 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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