MAP3K4 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K4
Entrez Gene ID	106893216
Full Name	mitogen-activated protein kinase kinase kinase 4
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014951001.1	XP_014806487.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_014951002.1	XP_014806488.1	mitogen-activated protein kinase kinase kinase 4 isoform X3
XM_014951005.1	XP_014806491.1	mitogen-activated protein kinase kinase kinase 4 isoform X6
XM_014950999.1	XP_014806485.1	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_014951003.1	XP_014806489.1	mitogen-activated protein kinase kinase kinase 4 isoform X4
XM_014951004.1	XP_014806490.1	mitogen-activated protein kinase kinase kinase 4 isoform X5
XM_014951006.1	XP_014806492.1	mitogen-activated protein kinase kinase kinase 4 isoform X7

Pathways from BioSystems
Gene Ontology

The following MAP3K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa71660	XM_014950999.1 Latest version!	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa71661	XM_014951001.1 Latest version!	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa71662	XM_014951002.1 Latest version!	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa71663	XM_014951003.1 Latest version!	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa71664	XM_014951004.1 Latest version!	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa71665	XM_014951005.1 Latest version!	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa71666	XM_014951006.1 Latest version!	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K4 ( XM_014950999.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014806485.1 Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa71660
Clone ID Related Accession (Same CDS sequence)	XM_014950999.1
Accession Version	XM_014950999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090922.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTTTGTACCT TGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAAGTACCT CGGTGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACGCCTGA AGGCTTCAGC ACGCGGAGTG TCCCTTGCGA TGCCCGGAGC 
CATTGCAGCG TTGCTGTCAG TCGCCCCGCT CCCGTCGGTA GCGACTCAGC TCAGCCCAAA 
GCTGCCAGCA GTGCAAATGA CACCAGGGGT TCCAGTGTCC CTGAAAATGA TCGACTGGCT 
TCAATAGCAG CTGAGTTGCA GTTCAGATCC CTTAGTCGCC ACTCCAGTCC CACTGAAGAG 
CGAGAAGAAC CAGCGTATCC AAAAGGAGAT TCAAGTGGCT CGTCCCGGCG GAGTTGGGAG 
CTCAGAACTT TAATCAACCA GACCAAAGAC ACTGCTTCCA AGCAAGCACC CATTGAAGCT 
GTTCAAAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAC 
ATCATTGGCC AAGTTTGTGA CACACCGAAA TCTTATGATA ATGTCATGCA TGTAGGTTTG 
AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA GTACGGGAAG 
GTCTATACCT GCATCAGTGT TGACACTGGA GAGCTGATGG CTATGAAGGA AATAAGATTT 
CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCCGATG AACTGAAGAT CTTTGAAGGC 
ATCAAACACC CTAATCTTGT TCGTTATTTT GGAGTGGAGC TCCATAGGGA AGAAATGTAC 
ATCTTCATGG AGTACTGTGA TGAGGGCACT CTGGAGGAGG TATCAAAACT GGGCCTTCAG 
GAGCATGTCA TTAGGCTCTA CTCAAAGCAA ATCACGGTTG CTATCAATGT ACTACATGAA 
CATGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCTGGACTG 
ATTAAACTAG GAGACTTTGG CTGCTCAGTG AAACTGAAGA ACAACACCCA GACAATGCCT 
GGAGAAGTGA ACAGTACTTT GGGGACTGCA GCGTACATGG CTCCAGAAGT AATTACCAGA 
GCTAAAGGAG AAGGGCACGG ACGCGCAGCG GACATCTGGA GCCTGGGCTG TGTTGTTATA 
GAGATGGTCA CGGGCAAGAG GCCTTGGCAC GAATACGAGC ACAACTTCCA GATCATGTAT 
AAAGTGGGGA TGGGTCATAA ACCTCCAATT CCTGACAAAG TGAGCCCAGA AGGGAAGGAC 
TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACTGCCAG CCAGCTCCTC 
GATCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014806485.1
CDS	840..5558
Translation	MRDVAVLVSAAAMEEPPPPGRKECKSEDATCKISESDPEELSDEASTDTFYGTSPPSTPRQMKRMSTKHQKNNIGRPASRSTLKEKMSVPTQSVHKDNGKTVENVEEHSYKQGKKIRDTLRTTERDHKKNVQCSFMLDSVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTSEGVCKATHGTSALGYSSYHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKDNDPEDDEVDRETEVKESSGTCTEESDGDEQISEENVEELRQAIKNNFSNKPNFDFQDHFSRRLDRLESEDDSASWAIPECSAEASCSQHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRSDHQLEFSEFPDPMLCSDYVQLLNSPPCCEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDLLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKTHELDDSWSSWEGQPVKVVPQVETVDTLRTMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHQEQTSSQPIIAKALQQLKSDALQLCNKISSAIDRVDHMFTAEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPCDARSHCSVAVSRPAPVGSDSAQPKAASSANDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPAYPKGDSSGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014950999.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014950999.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTTTGTACCT TGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAAGTACCT CGGTGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACGCCTGA AGGCTTCAGC ACGCGGAGTG TCCCTTGCGA TGCCCGGAGC 
CATTGCAGCG TTGCTGTCAG TCGCCCCGCT CCCGTCGGTA GCGACTCAGC TCAGCCCAAA 
GCTGCCAGCA GTGCAAATGA CACCAGGGGT TCCAGTGTCC CTGAAAATGA TCGACTGGCT 
TCAATAGCAG CTGAGTTGCA GTTCAGATCC CTTAGTCGCC ACTCCAGTCC CACTGAAGAG 
CGAGAAGAAC CAGCGTATCC AAAAGGAGAT TCAAGTGGCT CGTCCCGGCG GAGTTGGGAG 
CTCAGAACTT TAATCAACCA GACCAAAGAC ACTGCTTCCA AGCAAGCACC CATTGAAGCT 
GTTCAAAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAC 
ATCATTGGCC AAGTTTGTGA CACACCGAAA TCTTATGATA ATGTCATGCA TGTAGGTTTG 
AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA GTACGGGAAG 
GTCTATACCT GCATCAGTGT TGACACTGGA GAGCTGATGG CTATGAAGGA AATAAGATTT 
CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCCGATG AACTGAAGAT CTTTGAAGGC 
ATCAAACACC CTAATCTTGT TCGTTATTTT GGAGTGGAGC TCCATAGGGA AGAAATGTAC 
ATCTTCATGG AGTACTGTGA TGAGGGCACT CTGGAGGAGG TATCAAAACT GGGCCTTCAG 
GAGCATGTCA TTAGGCTCTA CTCAAAGCAA ATCACGGTTG CTATCAATGT ACTACATGAA 
CATGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCTGGACTG 
ATTAAACTAG GAGACTTTGG CTGCTCAGTG AAACTGAAGA ACAACACCCA GACAATGCCT 
GGAGAAGTGA ACAGTACTTT GGGGACTGCA GCGTACATGG CTCCAGAAGT AATTACCAGA 
GCTAAAGGAG AAGGGCACGG ACGCGCAGCG GACATCTGGA GCCTGGGCTG TGTTGTTATA 
GAGATGGTCA CGGGCAAGAG GCCTTGGCAC GAATACGAGC ACAACTTCCA GATCATGTAT 
AAAGTGGGGA TGGGTCATAA ACCTCCAATT CCTGACAAAG TGAGCCCAGA AGGGAAGGAC 
TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACTGCCAG CCAGCTCCTC 
GATCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014951001.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014806487.1 Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa71661
Clone ID Related Accession (Same CDS sequence)	XM_014951001.1
Accession Version	XM_014951001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090922.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG CTATTCATCG AAACAGTCCC 
CGTCCTGTAA AAGTACCTCG GTGCCATAGT GATCCACCAA ATCCTCATCT TATCATCCCC 
ACGCCTGAAG GCTTCAGCAC GCGGAGTGTC CCTTGCGATG CCCGGAGCCA TTGCAGCGTT 
GCTGTCAGTC GCCCCGCTCC CGTCGGTAGC GACTCAGCTC AGCCCAAAGC TGCCAGCAGT 
GCAAATGACA CCAGGGGTTC CAGTGTCCCT GAAAATGATC GACTGGCTTC AATAGCAGCT 
GAGTTGCAGT TCAGATCCCT TAGTCGCCAC TCCAGTCCCA CTGAAGAGCG AGAAGAACCA 
GCGTATCCAA AAGGAGATTC AAGTGGCTCG TCCCGGCGGA GTTGGGAGCT CAGAACTTTA 
ATCAACCAGA CCAAAGACAC TGCTTCCAAG CAAGCACCCA TTGAAGCTGT TCAAAAGTCT 
GTTCAGTTGT TTGAAGAAAG GAGATACAGA GAGATGAGGA GGAAGAACAT CATTGGCCAA 
GTTTGTGACA CACCGAAATC TTATGATAAT GTCATGCATG TAGGTTTGAG AAAAGTGACT 
TTCAAGTGGC AGAGAGGAAA CAAAATTGGT GAAGGCCAGT ACGGGAAGGT CTATACCTGC 
ATCAGTGTTG ACACTGGAGA GCTGATGGCT ATGAAGGAAA TAAGATTTCA ACCTAATGAT 
CACAAAACCA TCAAAGAAAC AGCCGATGAA CTGAAGATCT TTGAAGGCAT CAAACACCCT 
AATCTTGTTC GTTATTTTGG AGTGGAGCTC CATAGGGAAG AAATGTACAT CTTCATGGAG 
TACTGTGATG AGGGCACTCT GGAGGAGGTA TCAAAACTGG GCCTTCAGGA GCATGTCATT 
AGGCTCTACT CAAAGCAAAT CACGGTTGCT ATCAATGTAC TACATGAACA TGGTATTGTT 
CATCGAGACA TTAAAGGAGC CAACATCTTT CTTACCTCAT CTGGACTGAT TAAACTAGGA 
GACTTTGGCT GCTCAGTGAA ACTGAAGAAC AACACCCAGA CAATGCCTGG AGAAGTGAAC 
AGTACTTTGG GGACTGCAGC GTACATGGCT CCAGAAGTAA TTACCAGAGC TAAAGGAGAA 
GGGCACGGAC GCGCAGCGGA CATCTGGAGC CTGGGCTGTG TTGTTATAGA GATGGTCACG 
GGCAAGAGGC CTTGGCACGA ATACGAGCAC AACTTCCAGA TCATGTATAA AGTGGGGATG 
GGTCATAAAC CTCCAATTCC TGACAAAGTG AGCCCAGAAG GGAAGGACTT CCTTTGCCAC 
TGCCTGGAAA GTGACCCAAA GATGAGGTGG ACTGCCAGCC AGCTCCTCGA TCATCCTTTT 
GTCAAGGTTT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014806487.1
CDS	840..5546
Translation	MRDVAVLVSAAAMEEPPPPGRKECKSEDATCKISESDPEELSDEASTDTFYGTSPPSTPRQMKRMSTKHQKNNIGRPASRSTLKEKMSVPTQSVHKDNGKTVENVEEHSYKQGKKIRDTLRTTERDHKKNVQCSFMLDSVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTSEGVCKATHGTSALGYSSYHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKDNDPEDDEVDRETEVKESSGTCTEESDGDEQISEENVEELRQAIKNNFSNKPNFDFQDHFSRRLDRLESEDDSASWAIPECSAEASCSQHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRSDHQLEFSEFPDPMLCSDYVQLLNSPPCCEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDLLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKTHELDDSWSSWEGQPVKVVPQVETVDTLRTMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHQEQTSSQPIIAKALQQLKSDALQLCNKISSAIDRVDHMFTAEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPCDARSHCSVAVSRPAPVGSDSAQPKAASSANDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPAYPKGDSSGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014951001.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014951001.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG CTATTCATCG AAACAGTCCC 
CGTCCTGTAA AAGTACCTCG GTGCCATAGT GATCCACCAA ATCCTCATCT TATCATCCCC 
ACGCCTGAAG GCTTCAGCAC GCGGAGTGTC CCTTGCGATG CCCGGAGCCA TTGCAGCGTT 
GCTGTCAGTC GCCCCGCTCC CGTCGGTAGC GACTCAGCTC AGCCCAAAGC TGCCAGCAGT 
GCAAATGACA CCAGGGGTTC CAGTGTCCCT GAAAATGATC GACTGGCTTC AATAGCAGCT 
GAGTTGCAGT TCAGATCCCT TAGTCGCCAC TCCAGTCCCA CTGAAGAGCG AGAAGAACCA 
GCGTATCCAA AAGGAGATTC AAGTGGCTCG TCCCGGCGGA GTTGGGAGCT CAGAACTTTA 
ATCAACCAGA CCAAAGACAC TGCTTCCAAG CAAGCACCCA TTGAAGCTGT TCAAAAGTCT 
GTTCAGTTGT TTGAAGAAAG GAGATACAGA GAGATGAGGA GGAAGAACAT CATTGGCCAA 
GTTTGTGACA CACCGAAATC TTATGATAAT GTCATGCATG TAGGTTTGAG AAAAGTGACT 
TTCAAGTGGC AGAGAGGAAA CAAAATTGGT GAAGGCCAGT ACGGGAAGGT CTATACCTGC 
ATCAGTGTTG ACACTGGAGA GCTGATGGCT ATGAAGGAAA TAAGATTTCA ACCTAATGAT 
CACAAAACCA TCAAAGAAAC AGCCGATGAA CTGAAGATCT TTGAAGGCAT CAAACACCCT 
AATCTTGTTC GTTATTTTGG AGTGGAGCTC CATAGGGAAG AAATGTACAT CTTCATGGAG 
TACTGTGATG AGGGCACTCT GGAGGAGGTA TCAAAACTGG GCCTTCAGGA GCATGTCATT 
AGGCTCTACT CAAAGCAAAT CACGGTTGCT ATCAATGTAC TACATGAACA TGGTATTGTT 
CATCGAGACA TTAAAGGAGC CAACATCTTT CTTACCTCAT CTGGACTGAT TAAACTAGGA 
GACTTTGGCT GCTCAGTGAA ACTGAAGAAC AACACCCAGA CAATGCCTGG AGAAGTGAAC 
AGTACTTTGG GGACTGCAGC GTACATGGCT CCAGAAGTAA TTACCAGAGC TAAAGGAGAA 
GGGCACGGAC GCGCAGCGGA CATCTGGAGC CTGGGCTGTG TTGTTATAGA GATGGTCACG 
GGCAAGAGGC CTTGGCACGA ATACGAGCAC AACTTCCAGA TCATGTATAA AGTGGGGATG 
GGTCATAAAC CTCCAATTCC TGACAAAGTG AGCCCAGAAG GGAAGGACTT CCTTTGCCAC 
TGCCTGGAAA GTGACCCAAA GATGAGGTGG ACTGCCAGCC AGCTCCTCGA TCATCCTTTT 
GTCAAGGTTT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014951002.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014806488.1 Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

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CloneID	OCa71662
Clone ID Related Accession (Same CDS sequence)	XM_014951002.1
Accession Version	XM_014951002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090922.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTTTGTACCT TGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAAGTACCT CGGTGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACGCCTGA AGGCTTCAGG GGTTCCAGTG TCCCTGAAAA TGATCGACTG 
GCTTCAATAG CAGCTGAGTT GCAGTTCAGA TCCCTTAGTC GCCACTCCAG TCCCACTGAA 
GAGCGAGAAG AACCAGCGTA TCCAAAAGGA GATTCAAGTG GCTCGTCCCG GCGGAGTTGG 
GAGCTCAGAA CTTTAATCAA CCAGACCAAA GACACTGCTT CCAAGCAAGC ACCCATTGAA 
GCTGTTCAAA AGTCTGTTCA GTTGTTTGAA GAAAGGAGAT ACAGAGAGAT GAGGAGGAAG 
AACATCATTG GCCAAGTTTG TGACACACCG AAATCTTATG ATAATGTCAT GCATGTAGGT 
TTGAGAAAAG TGACTTTCAA GTGGCAGAGA GGAAACAAAA TTGGTGAAGG CCAGTACGGG 
AAGGTCTATA CCTGCATCAG TGTTGACACT GGAGAGCTGA TGGCTATGAA GGAAATAAGA 
TTTCAACCTA ATGATCACAA AACCATCAAA GAAACAGCCG ATGAACTGAA GATCTTTGAA 
GGCATCAAAC ACCCTAATCT TGTTCGTTAT TTTGGAGTGG AGCTCCATAG GGAAGAAATG 
TACATCTTCA TGGAGTACTG TGATGAGGGC ACTCTGGAGG AGGTATCAAA ACTGGGCCTT 
CAGGAGCATG TCATTAGGCT CTACTCAAAG CAAATCACGG TTGCTATCAA TGTACTACAT 
GAACATGGTA TTGTTCATCG AGACATTAAA GGAGCCAACA TCTTTCTTAC CTCATCTGGA 
CTGATTAAAC TAGGAGACTT TGGCTGCTCA GTGAAACTGA AGAACAACAC CCAGACAATG 
CCTGGAGAAG TGAACAGTAC TTTGGGGACT GCAGCGTACA TGGCTCCAGA AGTAATTACC 
AGAGCTAAAG GAGAAGGGCA CGGACGCGCA GCGGACATCT GGAGCCTGGG CTGTGTTGTT 
ATAGAGATGG TCACGGGCAA GAGGCCTTGG CACGAATACG AGCACAACTT CCAGATCATG 
TATAAAGTGG GGATGGGTCA TAAACCTCCA ATTCCTGACA AAGTGAGCCC AGAAGGGAAG 
GACTTCCTTT GCCACTGCCT GGAAAGTGAC CCAAAGATGA GGTGGACTGC CAGCCAGCTC 
CTCGATCATC CTTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014806488.1
CDS	840..5441
Translation	MRDVAVLVSAAAMEEPPPPGRKECKSEDATCKISESDPEELSDEASTDTFYGTSPPSTPRQMKRMSTKHQKNNIGRPASRSTLKEKMSVPTQSVHKDNGKTVENVEEHSYKQGKKIRDTLRTTERDHKKNVQCSFMLDSVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTSEGVCKATHGTSALGYSSYHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKDNDPEDDEVDRETEVKESSGTCTEESDGDEQISEENVEELRQAIKNNFSNKPNFDFQDHFSRRLDRLESEDDSASWAIPECSAEASCSQHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRSDHQLEFSEFPDPMLCSDYVQLLNSPPCCEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDLLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKTHELDDSWSSWEGQPVKVVPQVETVDTLRTMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHQEQTSSQPIIAKALQQLKSDALQLCNKISSAIDRVDHMFTAEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPAYPKGDSSGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014951002.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014951002.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTTTGTACCT TGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAAGTACCT CGGTGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACGCCTGA AGGCTTCAGG GGTTCCAGTG TCCCTGAAAA TGATCGACTG 
GCTTCAATAG CAGCTGAGTT GCAGTTCAGA TCCCTTAGTC GCCACTCCAG TCCCACTGAA 
GAGCGAGAAG AACCAGCGTA TCCAAAAGGA GATTCAAGTG GCTCGTCCCG GCGGAGTTGG 
GAGCTCAGAA CTTTAATCAA CCAGACCAAA GACACTGCTT CCAAGCAAGC ACCCATTGAA 
GCTGTTCAAA AGTCTGTTCA GTTGTTTGAA GAAAGGAGAT ACAGAGAGAT GAGGAGGAAG 
AACATCATTG GCCAAGTTTG TGACACACCG AAATCTTATG ATAATGTCAT GCATGTAGGT 
TTGAGAAAAG TGACTTTCAA GTGGCAGAGA GGAAACAAAA TTGGTGAAGG CCAGTACGGG 
AAGGTCTATA CCTGCATCAG TGTTGACACT GGAGAGCTGA TGGCTATGAA GGAAATAAGA 
TTTCAACCTA ATGATCACAA AACCATCAAA GAAACAGCCG ATGAACTGAA GATCTTTGAA 
GGCATCAAAC ACCCTAATCT TGTTCGTTAT TTTGGAGTGG AGCTCCATAG GGAAGAAATG 
TACATCTTCA TGGAGTACTG TGATGAGGGC ACTCTGGAGG AGGTATCAAA ACTGGGCCTT 
CAGGAGCATG TCATTAGGCT CTACTCAAAG CAAATCACGG TTGCTATCAA TGTACTACAT 
GAACATGGTA TTGTTCATCG AGACATTAAA GGAGCCAACA TCTTTCTTAC CTCATCTGGA 
CTGATTAAAC TAGGAGACTT TGGCTGCTCA GTGAAACTGA AGAACAACAC CCAGACAATG 
CCTGGAGAAG TGAACAGTAC TTTGGGGACT GCAGCGTACA TGGCTCCAGA AGTAATTACC 
AGAGCTAAAG GAGAAGGGCA CGGACGCGCA GCGGACATCT GGAGCCTGGG CTGTGTTGTT 
ATAGAGATGG TCACGGGCAA GAGGCCTTGG CACGAATACG AGCACAACTT CCAGATCATG 
TATAAAGTGG GGATGGGTCA TAAACCTCCA ATTCCTGACA AAGTGAGCCC AGAAGGGAAG 
GACTTCCTTT GCCACTGCCT GGAAAGTGAC CCAAAGATGA GGTGGACTGC CAGCCAGCTC 
CTCGATCATC CTTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014951003.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014806489.1 Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

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CloneID	OCa71663
Clone ID Related Accession (Same CDS sequence)	XM_014951003.1
Accession Version	XM_014951003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090922.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG GAGGTCTGTT ATAGAGACGA GCCGAGCACT CAAAGAACTG 
TTTCATGAAG CTAGAGAAAG GGCCTCCAAA GCTCTTGGTT TTGCTAAAAT GCTGAGGAAG 
GATCTTGAAA TAGCAGCAGA GTTTGCATTG TCAGCCCCTG TGCGAGATCT TCTGAATGCT 
CTGAAAACAA AAGAGTATGT GAAGGTACAA ATCCCTGGAC TCGAGAATTT ACAAGTATTT 
GTACCAAATA GTATTGCAGG TGAGAAATCT GTGATTTTGC AGCTCCTCAA CGCAGCTGCT 
GGAAAGGATT GCTCAAAGGA TTCGGATGAA GTTGCGTATG AAGCCTACTT ACTGATGACC 
AAACACAGCG ATAAAACCCA TGAACTGGAT GACAGCTGGA GTTCGTGGGA GGGCCAGCCA 
GTCAAAGTAG TGCCACAAGT AGAAACTGTT GATACACTGA GAACCATGCA GGTTGATAAC 
CTTCTCCTTG TAGTCATTCA ATCTGCCCAC CTTGTGAGTC AGAGGAAAGC TTTCCAGCAG 
TCTATCGAAG GGCTAATTTC TCTGCACCAG GAACAGACAT CCAGCCAACC GATCATCGCC 
AAAGCTCTAC AACAGCTAAA GAGTGATGCA TTACAGCTAT GCAATAAGAT AAGCAGTGCC 
ATTGACAGAG TTGATCACAT GTTCACGGCT GAGTTTGATG CTGAAGTGGA TGAATCTGAA 
TCTGCCACTT TGCAGCAATA CTACCGGGAA GCCATGATTC AGGGTTATAA TTTTGGTTTT 
GAGTACCATA AAGAAGTTGT TCGTCTGATG TCTGGAGAAT TCAGGCAGAA AATTGGAGAC 
AAGTATATCA GCTTTGCCAG AAAATGGATG AATTATGTGC TAACAAAATG TGAGAGCGGC 
CGAGGCACTC GACCCAGATG GGCAACTCAA GGTTTTGATT TTCTTCAAGC CATTGAGCCG 
GCATTTATTT CAGCTCTCCC GGAAGATGAC TTCTTGAGTT TACAAGCTCT GATGAATGAG 
TGCATCGGCC ATGTTATTGG AAAACCTCAC AGTCCTGTTA CAGGTTTGTA CCTTGCTATT 
CATCGAAACA GTCCCCGTCC TGTAAAAGTA CCTCGGTGCC ATAGTGATCC ACCAAATCCT 
CATCTTATCA TCCCCACGCC TGAAGGCTTC AGCACGCGGA GTGTCCCTTG CGATGCCCGG 
AGCCATTGCA GCGTTGCTGT CAGTCGCCCC GCTCCCGTCG GTAGCGACTC AGCTCAGCCC 
AAAGCTGCCA GCAGTGCAAA TGACACCAGG GGTTCCAGTG TCCCTGAAAA TGATCGACTG 
GCTTCAATAG CAGCTGAGTT GCAGTTCAGA TCCCTTAGTC GCCACTCCAG TCCCACTGAA 
GAGCGAGAAG AACCAGCGTA TCCAAAAGGA GATTCAAGTG GCTCGTCCCG GCGGAGTTGG 
GAGCTCAGAA CTTTAATCAA CCAGACCAAA GACACTGCTT CCAAGCAAGC ACCCATTGAA 
GCTGTTCAAA AGTCTGTTCA GTTGTTTGAA GAAAGGAGAT ACAGAGAGAT GAGGAGGAAG 
AACATCATTG GCCAAGTTTG TGACACACCG AAATCTTATG ATAATGTCAT GCATGTAGGT 
TTGAGAAAAG TGACTTTCAA GTGGCAGAGA GGAAACAAAA TTGGTGAAGG CCAGTACGGG 
AAGGTCTATA CCTGCATCAG TGTTGACACT GGAGAGCTGA TGGCTATGAA GGAAATAAGA 
TTTCAACCTA ATGATCACAA AACCATCAAA GAAACAGCCG ATGAACTGAA GATCTTTGAA 
GGCATCAAAC ACCCTAATCT TGTTCGTTAT TTTGGAGTGG AGCTCCATAG GGAAGAAATG 
TACATCTTCA TGGAGTACTG TGATGAGGGC ACTCTGGAGG AGGTATCAAA ACTGGGCCTT 
CAGGAGCATG TCATTAGGCT CTACTCAAAG CAAATCACGG TTGCTATCAA TGTACTACAT 
GAACATGGTA TTGTTCATCG AGACATTAAA GGAGCCAACA TCTTTCTTAC CTCATCTGGA 
CTGATTAAAC TAGGAGACTT TGGCTGCTCA GTGAAACTGA AGAACAACAC CCAGACAATG 
CCTGGAGAAG TGAACAGTAC TTTGGGGACT GCAGCGTACA TGGCTCCAGA AGTAATTACC 
AGAGCTAAAG GAGAAGGGCA CGGACGCGCA GCGGACATCT GGAGCCTGGG CTGTGTTGTT 
ATAGAGATGG TCACGGGCAA GAGGCCTTGG CACGAATACG AGCACAACTT CCAGATCATG 
TATAAAGTGG GGATGGGTCA TAAACCTCCA ATTCCTGACA AAGTGAGCCC AGAAGGGAAG 
GACTTCCTTT GCCACTGCCT GGAAAGTGAC CCAAAGATGA GGTGGACTGC CAGCCAGCTC 
CTCGATCATC CTTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014806489.1
CDS	841..5442
Translation	MRDVAVLVSAAAMEEPPPPGRKECKSEDATCKISESDPEELSDEASTDTFYGTSPPSTPRQMKRMSTKHQKNNIGRPASRSTLKEKMSVPTQSVHKDNGKTVENVEEHSYKQGKKIRDTLRTTERDHKKNVQCSFMLDSVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTSEGVCKATHGTSALGYSSYHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKDNDPEDDEVDRETEVKESSGTCTEESDGDEQISEENVEELRQAIKNNFSNKPNFDFQDHFSRRLDRLESEDDSASWAIPECSAEASCSQHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRSDHQLEFSEFPDPMLCSDYVQLLNSPPCCEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFWRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDLLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKTHELDDSWSSWEGQPVKVVPQVETVDTLRTMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHQEQTSSQPIIAKALQQLKSDALQLCNKISSAIDRVDHMFTAEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPCDARSHCSVAVSRPAPVGSDSAQPKAASSANDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPAYPKGDSSGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014951003.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014951003.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG GAGGTCTGTT ATAGAGACGA GCCGAGCACT CAAAGAACTG 
TTTCATGAAG CTAGAGAAAG GGCCTCCAAA GCTCTTGGTT TTGCTAAAAT GCTGAGGAAG 
GATCTTGAAA TAGCAGCAGA GTTTGCATTG TCAGCCCCTG TGCGAGATCT TCTGAATGCT 
CTGAAAACAA AAGAGTATGT GAAGGTACAA ATCCCTGGAC TCGAGAATTT ACAAGTATTT 
GTACCAAATA GTATTGCAGG TGAGAAATCT GTGATTTTGC AGCTCCTCAA CGCAGCTGCT 
GGAAAGGATT GCTCAAAGGA TTCGGATGAA GTTGCGTATG AAGCCTACTT ACTGATGACC 
AAACACAGCG ATAAAACCCA TGAACTGGAT GACAGCTGGA GTTCGTGGGA GGGCCAGCCA 
GTCAAAGTAG TGCCACAAGT AGAAACTGTT GATACACTGA GAACCATGCA GGTTGATAAC 
CTTCTCCTTG TAGTCATTCA ATCTGCCCAC CTTGTGAGTC AGAGGAAAGC TTTCCAGCAG 
TCTATCGAAG GGCTAATTTC TCTGCACCAG GAACAGACAT CCAGCCAACC GATCATCGCC 
AAAGCTCTAC AACAGCTAAA GAGTGATGCA TTACAGCTAT GCAATAAGAT AAGCAGTGCC 
ATTGACAGAG TTGATCACAT GTTCACGGCT GAGTTTGATG CTGAAGTGGA TGAATCTGAA 
TCTGCCACTT TGCAGCAATA CTACCGGGAA GCCATGATTC AGGGTTATAA TTTTGGTTTT 
GAGTACCATA AAGAAGTTGT TCGTCTGATG TCTGGAGAAT TCAGGCAGAA AATTGGAGAC 
AAGTATATCA GCTTTGCCAG AAAATGGATG AATTATGTGC TAACAAAATG TGAGAGCGGC 
CGAGGCACTC GACCCAGATG GGCAACTCAA GGTTTTGATT TTCTTCAAGC CATTGAGCCG 
GCATTTATTT CAGCTCTCCC GGAAGATGAC TTCTTGAGTT TACAAGCTCT GATGAATGAG 
TGCATCGGCC ATGTTATTGG AAAACCTCAC AGTCCTGTTA CAGGTTTGTA CCTTGCTATT 
CATCGAAACA GTCCCCGTCC TGTAAAAGTA CCTCGGTGCC ATAGTGATCC ACCAAATCCT 
CATCTTATCA TCCCCACGCC TGAAGGCTTC AGCACGCGGA GTGTCCCTTG CGATGCCCGG 
AGCCATTGCA GCGTTGCTGT CAGTCGCCCC GCTCCCGTCG GTAGCGACTC AGCTCAGCCC 
AAAGCTGCCA GCAGTGCAAA TGACACCAGG GGTTCCAGTG TCCCTGAAAA TGATCGACTG 
GCTTCAATAG CAGCTGAGTT GCAGTTCAGA TCCCTTAGTC GCCACTCCAG TCCCACTGAA 
GAGCGAGAAG AACCAGCGTA TCCAAAAGGA GATTCAAGTG GCTCGTCCCG GCGGAGTTGG 
GAGCTCAGAA CTTTAATCAA CCAGACCAAA GACACTGCTT CCAAGCAAGC ACCCATTGAA 
GCTGTTCAAA AGTCTGTTCA GTTGTTTGAA GAAAGGAGAT ACAGAGAGAT GAGGAGGAAG 
AACATCATTG GCCAAGTTTG TGACACACCG AAATCTTATG ATAATGTCAT GCATGTAGGT 
TTGAGAAAAG TGACTTTCAA GTGGCAGAGA GGAAACAAAA TTGGTGAAGG CCAGTACGGG 
AAGGTCTATA CCTGCATCAG TGTTGACACT GGAGAGCTGA TGGCTATGAA GGAAATAAGA 
TTTCAACCTA ATGATCACAA AACCATCAAA GAAACAGCCG ATGAACTGAA GATCTTTGAA 
GGCATCAAAC ACCCTAATCT TGTTCGTTAT TTTGGAGTGG AGCTCCATAG GGAAGAAATG 
TACATCTTCA TGGAGTACTG TGATGAGGGC ACTCTGGAGG AGGTATCAAA ACTGGGCCTT 
CAGGAGCATG TCATTAGGCT CTACTCAAAG CAAATCACGG TTGCTATCAA TGTACTACAT 
GAACATGGTA TTGTTCATCG AGACATTAAA GGAGCCAACA TCTTTCTTAC CTCATCTGGA 
CTGATTAAAC TAGGAGACTT TGGCTGCTCA GTGAAACTGA AGAACAACAC CCAGACAATG 
CCTGGAGAAG TGAACAGTAC TTTGGGGACT GCAGCGTACA TGGCTCCAGA AGTAATTACC 
AGAGCTAAAG GAGAAGGGCA CGGACGCGCA GCGGACATCT GGAGCCTGGG CTGTGTTGTT 
ATAGAGATGG TCACGGGCAA GAGGCCTTGG CACGAATACG AGCACAACTT CCAGATCATG 
TATAAAGTGG GGATGGGTCA TAAACCTCCA ATTCCTGACA AAGTGAGCCC AGAAGGGAAG 
GACTTCCTTT GCCACTGCCT GGAAAGTGAC CCAAAGATGA GGTGGACTGC CAGCCAGCTC 
CTCGATCATC CTTTTGTCAA GGTTTGCACA GATGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014951004.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014806490.1 Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.

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CloneID	OCa71664
Clone ID Related Accession (Same CDS sequence)	XM_014951004.1
Accession Version	XM_014951004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090922.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG CTATTCATCG AAACAGTCCC 
CGTCCTGTAA AAGTACCTCG GTGCCATAGT GATCCACCAA ATCCTCATCT TATCATCCCC 
ACGCCTGAAG GCTTCAGGGG TTCCAGTGTC CCTGAAAATG ATCGACTGGC TTCAATAGCA 
GCTGAGTTGC AGTTCAGATC CCTTAGTCGC CACTCCAGTC CCACTGAAGA GCGAGAAGAA 
CCAGCGTATC CAAAAGGAGA TTCAAGTGGC TCGTCCCGGC GGAGTTGGGA GCTCAGAACT 
TTAATCAACC AGACCAAAGA CACTGCTTCC AAGCAAGCAC CCATTGAAGC TGTTCAAAAG 
TCTGTTCAGT TGTTTGAAGA AAGGAGATAC AGAGAGATGA GGAGGAAGAA CATCATTGGC 
CAAGTTTGTG ACACACCGAA ATCTTATGAT AATGTCATGC ATGTAGGTTT GAGAAAAGTG 
ACTTTCAAGT GGCAGAGAGG AAACAAAATT GGTGAAGGCC AGTACGGGAA GGTCTATACC 
TGCATCAGTG TTGACACTGG AGAGCTGATG GCTATGAAGG AAATAAGATT TCAACCTAAT 
GATCACAAAA CCATCAAAGA AACAGCCGAT GAACTGAAGA TCTTTGAAGG CATCAAACAC 
CCTAATCTTG TTCGTTATTT TGGAGTGGAG CTCCATAGGG AAGAAATGTA CATCTTCATG 
GAGTACTGTG ATGAGGGCAC TCTGGAGGAG GTATCAAAAC TGGGCCTTCA GGAGCATGTC 
ATTAGGCTCT ACTCAAAGCA AATCACGGTT GCTATCAATG TACTACATGA ACATGGTATT 
GTTCATCGAG ACATTAAAGG AGCCAACATC TTTCTTACCT CATCTGGACT GATTAAACTA 
GGAGACTTTG GCTGCTCAGT GAAACTGAAG AACAACACCC AGACAATGCC TGGAGAAGTG 
AACAGTACTT TGGGGACTGC AGCGTACATG GCTCCAGAAG TAATTACCAG AGCTAAAGGA 
GAAGGGCACG GACGCGCAGC GGACATCTGG AGCCTGGGCT GTGTTGTTAT AGAGATGGTC 
ACGGGCAAGA GGCCTTGGCA CGAATACGAG CACAACTTCC AGATCATGTA TAAAGTGGGG 
ATGGGTCATA AACCTCCAAT TCCTGACAAA GTGAGCCCAG AAGGGAAGGA CTTCCTTTGC 
CACTGCCTGG AAAGTGACCC AAAGATGAGG TGGACTGCCA GCCAGCTCCT CGATCATCCT 
TTTGTCAAGG TTTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014806490.1
CDS	840..5429
Translation	MRDVAVLVSAAAMEEPPPPGRKECKSEDATCKISESDPEELSDEASTDTFYGTSPPSTPRQMKRMSTKHQKNNIGRPASRSTLKEKMSVPTQSVHKDNGKTVENVEEHSYKQGKKIRDTLRTTERDHKKNVQCSFMLDSVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTSEGVCKATHGTSALGYSSYHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKDNDPEDDEVDRETEVKESSGTCTEESDGDEQISEENVEELRQAIKNNFSNKPNFDFQDHFSRRLDRLESEDDSASWAIPECSAEASCSQHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRSDHQLEFSEFPDPMLCSDYVQLLNSPPCCEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDLLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKTHELDDSWSSWEGQPVKVVPQVETVDTLRTMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHQEQTSSQPIIAKALQQLKSDALQLCNKISSAIDRVDHMFTAEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPAYPKGDSSGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014951004.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014951004.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG CTATTCATCG AAACAGTCCC 
CGTCCTGTAA AAGTACCTCG GTGCCATAGT GATCCACCAA ATCCTCATCT TATCATCCCC 
ACGCCTGAAG GCTTCAGGGG TTCCAGTGTC CCTGAAAATG ATCGACTGGC TTCAATAGCA 
GCTGAGTTGC AGTTCAGATC CCTTAGTCGC CACTCCAGTC CCACTGAAGA GCGAGAAGAA 
CCAGCGTATC CAAAAGGAGA TTCAAGTGGC TCGTCCCGGC GGAGTTGGGA GCTCAGAACT 
TTAATCAACC AGACCAAAGA CACTGCTTCC AAGCAAGCAC CCATTGAAGC TGTTCAAAAG 
TCTGTTCAGT TGTTTGAAGA AAGGAGATAC AGAGAGATGA GGAGGAAGAA CATCATTGGC 
CAAGTTTGTG ACACACCGAA ATCTTATGAT AATGTCATGC ATGTAGGTTT GAGAAAAGTG 
ACTTTCAAGT GGCAGAGAGG AAACAAAATT GGTGAAGGCC AGTACGGGAA GGTCTATACC 
TGCATCAGTG TTGACACTGG AGAGCTGATG GCTATGAAGG AAATAAGATT TCAACCTAAT 
GATCACAAAA CCATCAAAGA AACAGCCGAT GAACTGAAGA TCTTTGAAGG CATCAAACAC 
CCTAATCTTG TTCGTTATTT TGGAGTGGAG CTCCATAGGG AAGAAATGTA CATCTTCATG 
GAGTACTGTG ATGAGGGCAC TCTGGAGGAG GTATCAAAAC TGGGCCTTCA GGAGCATGTC 
ATTAGGCTCT ACTCAAAGCA AATCACGGTT GCTATCAATG TACTACATGA ACATGGTATT 
GTTCATCGAG ACATTAAAGG AGCCAACATC TTTCTTACCT CATCTGGACT GATTAAACTA 
GGAGACTTTG GCTGCTCAGT GAAACTGAAG AACAACACCC AGACAATGCC TGGAGAAGTG 
AACAGTACTT TGGGGACTGC AGCGTACATG GCTCCAGAAG TAATTACCAG AGCTAAAGGA 
GAAGGGCACG GACGCGCAGC GGACATCTGG AGCCTGGGCT GTGTTGTTAT AGAGATGGTC 
ACGGGCAAGA GGCCTTGGCA CGAATACGAG CACAACTTCC AGATCATGTA TAAAGTGGGG 
ATGGGTCATA AACCTCCAAT TCCTGACAAA GTGAGCCCAG AAGGGAAGGA CTTCCTTTGC 
CACTGCCTGG AAAGTGACCC AAAGATGAGG TGGACTGCCA GCCAGCTCCT CGATCATCCT 
TTTGTCAAGG TTTGCACAGA TGAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014951005.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014806491.1 Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCa71665
Clone ID Related Accession (Same CDS sequence)	XM_014951005.1
Accession Version	XM_014951005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090922.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGTA TGTCAACAAA GCATCAGAAA AATAACATTG GGAGACCTGC TAGCCGCTCC 
ACTTTGAAAG AAAAAATGAG TGTGCCAACT CAGTCTGTGC ATAAAGACAA CGGGAAAACC 
GTTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGAAAAA AGATCCGAGA CACCCTAAGG 
ACAACAGAAC GAGATCACAA GAAAAATGTG CAGTGTTCAT TTATGTTAGA CTCAGTTGGT 
GGGTCTCTGC CAAAAAAACC AATCCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT 
GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATACCCAG AACTGCTCGC 
CAGACTTCTA GGACTGATTG CCCAGCAGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT 
TTGTTAAAAC TTACCTCAGT CTCAAAGAAA AAGGACAGGG AGCAAAGAGG GCAGGAAAAC 
ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTGGAGCT ACAAGCTTGG 
CATGCTGGCC GGAGCATTAA TGACCAGGAC CTCTATCTCT ATGCAGCCCG TCAAGCTATC 
CCTGATATCA TCAATGAAAT CCTCACCTTC AAAGTGAACT ATGGAAGCTT CTCCTGTGTA 
AAAAATGGAG CCAGTCTCAA TGGTACTTCA GAAGGAGTTT GCAAAGCAAC ACATGGAACT 
AGTGCTTTGG GTTACTCAAG TTACCATGAA CACCTCCAAC GCCAGCGGGT CTCATTTGAG 
CAAGTGAAGC GGATAATGGA GCTGCTAGAG TATATAGAAG CACTTTATCC ATCTCTGCAG 
GCTCTTCAAA AGGACTATGA AAAGTATGCT GCAAAGGACT TCCAAGACAG AGTGCAGGCA 
CTCTGTCTAT GGTTAAATAT TACAAAAGAC TTAAATCAAA AACTAAGGAT TATGGGAACT 
GTATTGGGCA TCAAAAATCT TTCTGACATT GGCTGGCCAG TGTTTGAAAT TCCTTCTCCT 
CGACCATCTA AGGACAATGA TCCAGAAGAT GATGAAGTTG ATAGGGAAAC AGAAGTAAAG 
GAATCCTCAG GAACATGCAC AGAGGAGAGC GATGGTGATG AACAAATCTC TGAGGAGAAT 
GTTGAGGAAC TTAGACAAGC CATCAAGAAC AATTTTAGTA ATAAGCCAAA TTTTGACTTT 
CAGGACCATT TTTCAAGAAG GCTTGATAGG CTTGAATCTG AGGATGACTC TGCCTCTTGG 
GCTATTCCAG AATGCAGTGC TGAGGCAAGC TGCAGCCAAC ACTGTTTGAC CTCTATTTAC 
AGGCCGTTTG TAGATAAAGC ACTGAAGCAA ATGGGGTTAA GGAAACTAAT TTTAAGACTG 
CACAAGCTAA TGCATGGTTC ATTGCAAAGG GCCCGCATGG CGTTGGTAAG GAGTGACCAC 
CAGCTGGAGT TTTCCGAATT TCCAGATCCG ATGTTGTGTT CAGATTATGT GCAGTTACTA 
AATTCCCCAC CTTGTTGTGA GCAAAAATGC AGCACTGTAT CATGGGAGGA GCTGAAATCC 
ATGGATTTAC CATCTTTTGA ACCTGCATTC TTGGTCCTCT GCCGAGTTCT GCTCAATGTT 
ATCCATGAAT GTCTAAAGCT AAGGTTGGAA CAAAGACCTG CTGGGGAGCC TTCTCTTTTG 
AGTATTAAAC AGCTAGTGAG AGAGTGTAAA GAGGTTCTGA AGGGTGGCCT GTTAATGAAG 
CAATATTACC AGTTTATGTT GCGGGAGGTG TTAGATGACT TACAAAAGAT TGATTGCAAC 
ATCGATTCTT TTGAAGAGGA CCTGCATAAA ATGTTAATGG TTTACTTTGA TTATATGCGA 
AGCTGGATCC AAATGCTACA GCAGTTACCT CAAGCGTCTC ATAGTTTAAA AAATCTGTTG 
GAGGAAGAAT GGAATTTCAC CAAAGAAATA ACTCCTTACA TAAGAGGAGG TGAAGCTCAA 
GCTGGAAAAC TATTCTGTGA TATTGCAGGA ATGCTGCTGA AATCTACAGG AATATTTTTA 
GATTCTGGAC TACAAGACAG TTGTGATGAA TTTTGGGCCA GTGCCGATGA CAGTACTGCA 
TCAGATGAAA TCAGGAGGTC TGTTATAGAG ACGAGCCGAG CACTCAAAGA ACTGTTTCAT 
GAAGCTAGAG AAAGGGCCTC CAAAGCTCTT GGTTTTGCTA AAATGCTGAG GAAGGATCTT 
GAAATAGCAG CAGAGTTTGC ATTGTCAGCC CCTGTGCGAG ATCTTCTGAA TGCTCTGAAA 
ACAAAAGAGT ATGTGAAGGT ACAAATCCCT GGACTCGAGA ATTTACAAGT ATTTGTACCA 
AATAGTATTG CAGGTGAGAA ATCTGTGATT TTGCAGCTCC TCAACGCAGC TGCTGGAAAG 
GATTGCTCAA AGGATTCGGA TGAAGTTGCG TATGAAGCCT ACTTACTGAT GACCAAACAC 
AGCGATAAAA CCCATGAACT GGATGACAGC TGGAGTTCGT GGGAGGGCCA GCCAGTCAAA 
GTAGTGCCAC AAGTAGAAAC TGTTGATACA CTGAGAACCA TGCAGGTTGA TAACCTTCTC 
CTTGTAGTCA TTCAATCTGC CCACCTTGTG AGTCAGAGGA AAGCTTTCCA GCAGTCTATC 
GAAGGGCTAA TTTCTCTGCA CCAGGAACAG ACATCCAGCC AACCGATCAT CGCCAAAGCT 
CTACAACAGC TAAAGAGTGA TGCATTACAG CTATGCAATA AGATAAGCAG TGCCATTGAC 
AGAGTTGATC ACATGTTCAC GGCTGAGTTT GATGCTGAAG TGGATGAATC TGAATCTGCC 
ACTTTGCAGC AATACTACCG GGAAGCCATG ATTCAGGGTT ATAATTTTGG TTTTGAGTAC 
CATAAAGAAG TTGTTCGTCT GATGTCTGGA GAATTCAGGC AGAAAATTGG AGACAAGTAT 
ATCAGCTTTG CCAGAAAATG GATGAATTAT GTGCTAACAA AATGTGAGAG CGGCCGAGGC 
ACTCGACCCA GATGGGCAAC TCAAGGTTTT GATTTTCTTC AAGCCATTGA GCCGGCATTT 
ATTTCAGCTC TCCCGGAAGA TGACTTCTTG AGTTTACAAG CTCTGATGAA TGAGTGCATC 
GGCCATGTTA TTGGAAAACC TCACAGTCCT GTTACAGGTT TGTACCTTGC TATTCATCGA 
AACAGTCCCC GTCCTGTAAA AGTACCTCGG TGCCATAGTG ATCCACCAAA TCCTCATCTT 
ATCATCCCCA CGCCTGAAGG CTTCAGCACG CGGAGTGTCC CTTGCGATGC CCGGAGCCAT 
TGCAGCGTTG CTGTCAGTCG CCCCGCTCCC GTCGGTAGCG ACTCAGCTCA GCCCAAAGCT 
GCCAGCAGTG CAAATGACAC CAGGGGTTCC AGTGTCCCTG AAAATGATCG ACTGGCTTCA 
ATAGCAGCTG AGTTGCAGTT CAGATCCCTT AGTCGCCACT CCAGTCCCAC TGAAGAGCGA 
GAAGAACCAG CGTATCCAAA AGGAGATTCA AGTGGCTCGT CCCGGCGGAG TTGGGAGCTC 
AGAACTTTAA TCAACCAGAC CAAAGACACT GCTTCCAAGC AAGCACCCAT TGAAGCTGTT 
CAAAAGTCTG TTCAGTTGTT TGAAGAAAGG AGATACAGAG AGATGAGGAG GAAGAACATC 
ATTGGCCAAG TTTGTGACAC ACCGAAATCT TATGATAATG TCATGCATGT AGGTTTGAGA 
AAAGTGACTT TCAAGTGGCA GAGAGGAAAC AAAATTGGTG AAGGCCAGTA CGGGAAGGTC 
TATACCTGCA TCAGTGTTGA CACTGGAGAG CTGATGGCTA TGAAGGAAAT AAGATTTCAA 
CCTAATGATC ACAAAACCAT CAAAGAAACA GCCGATGAAC TGAAGATCTT TGAAGGCATC 
AAACACCCTA ATCTTGTTCG TTATTTTGGA GTGGAGCTCC ATAGGGAAGA AATGTACATC 
TTCATGGAGT ACTGTGATGA GGGCACTCTG GAGGAGGTAT CAAAACTGGG CCTTCAGGAG 
CATGTCATTA GGCTCTACTC AAAGCAAATC ACGGTTGCTA TCAATGTACT ACATGAACAT 
GGTATTGTTC ATCGAGACAT TAAAGGAGCC AACATCTTTC TTACCTCATC TGGACTGATT 
AAACTAGGAG ACTTTGGCTG CTCAGTGAAA CTGAAGAACA ACACCCAGAC AATGCCTGGA 
GAAGTGAACA GTACTTTGGG GACTGCAGCG TACATGGCTC CAGAAGTAAT TACCAGAGCT 
AAAGGAGAAG GGCACGGACG CGCAGCGGAC ATCTGGAGCC TGGGCTGTGT TGTTATAGAG 
ATGGTCACGG GCAAGAGGCC TTGGCACGAA TACGAGCACA ACTTCCAGAT CATGTATAAA 
GTGGGGATGG GTCATAAACC TCCAATTCCT GACAAAGTGA GCCCAGAAGG GAAGGACTTC 
CTTTGCCACT GCCTGGAAAG TGACCCAAAG ATGAGGTGGA CTGCCAGCCA GCTCCTCGAT 
CATCCTTTTG TCAAGGTTTG CACAGATGAA GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014806491.1
CDS	160..4695
Translation	MKRMSTKHQKNNIGRPASRSTLKEKMSVPTQSVHKDNGKTVENVEEHSYKQGKKIRDTLRTTERDHKKNVQCSFMLDSVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTSEGVCKATHGTSALGYSSYHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKDNDPEDDEVDRETEVKESSGTCTEESDGDEQISEENVEELRQAIKNNFSNKPNFDFQDHFSRRLDRLESEDDSASWAIPECSAEASCSQHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRSDHQLEFSEFPDPMLCSDYVQLLNSPPCCEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDLLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKTHELDDSWSSWEGQPVKVVPQVETVDTLRTMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHQEQTSSQPIIAKALQQLKSDALQLCNKISSAIDRVDHMFTAEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPCDARSHCSVAVSRPAPVGSDSAQPKAASSANDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPAYPKGDSSGSSRRSWELRTLINQTKDTASKQAPIEAVQKSVQLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014951005.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014951005.1

ORF Insert Sequence:

ATGAAGCGTA TGTCAACAAA GCATCAGAAA AATAACATTG GGAGACCTGC TAGCCGCTCC 
ACTTTGAAAG AAAAAATGAG TGTGCCAACT CAGTCTGTGC ATAAAGACAA CGGGAAAACC 
GTTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGAAAAA AGATCCGAGA CACCCTAAGG 
ACAACAGAAC GAGATCACAA GAAAAATGTG CAGTGTTCAT TTATGTTAGA CTCAGTTGGT 
GGGTCTCTGC CAAAAAAACC AATCCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT 
GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATACCCAG AACTGCTCGC 
CAGACTTCTA GGACTGATTG CCCAGCAGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT 
TTGTTAAAAC TTACCTCAGT CTCAAAGAAA AAGGACAGGG AGCAAAGAGG GCAGGAAAAC 
ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTGGAGCT ACAAGCTTGG 
CATGCTGGCC GGAGCATTAA TGACCAGGAC CTCTATCTCT ATGCAGCCCG TCAAGCTATC 
CCTGATATCA TCAATGAAAT CCTCACCTTC AAAGTGAACT ATGGAAGCTT CTCCTGTGTA 
AAAAATGGAG CCAGTCTCAA TGGTACTTCA GAAGGAGTTT GCAAAGCAAC ACATGGAACT 
AGTGCTTTGG GTTACTCAAG TTACCATGAA CACCTCCAAC GCCAGCGGGT CTCATTTGAG 
CAAGTGAAGC GGATAATGGA GCTGCTAGAG TATATAGAAG CACTTTATCC ATCTCTGCAG 
GCTCTTCAAA AGGACTATGA AAAGTATGCT GCAAAGGACT TCCAAGACAG AGTGCAGGCA 
CTCTGTCTAT GGTTAAATAT TACAAAAGAC TTAAATCAAA AACTAAGGAT TATGGGAACT 
GTATTGGGCA TCAAAAATCT TTCTGACATT GGCTGGCCAG TGTTTGAAAT TCCTTCTCCT 
CGACCATCTA AGGACAATGA TCCAGAAGAT GATGAAGTTG ATAGGGAAAC AGAAGTAAAG 
GAATCCTCAG GAACATGCAC AGAGGAGAGC GATGGTGATG AACAAATCTC TGAGGAGAAT 
GTTGAGGAAC TTAGACAAGC CATCAAGAAC AATTTTAGTA ATAAGCCAAA TTTTGACTTT 
CAGGACCATT TTTCAAGAAG GCTTGATAGG CTTGAATCTG AGGATGACTC TGCCTCTTGG 
GCTATTCCAG AATGCAGTGC TGAGGCAAGC TGCAGCCAAC ACTGTTTGAC CTCTATTTAC 
AGGCCGTTTG TAGATAAAGC ACTGAAGCAA ATGGGGTTAA GGAAACTAAT TTTAAGACTG 
CACAAGCTAA TGCATGGTTC ATTGCAAAGG GCCCGCATGG CGTTGGTAAG GAGTGACCAC 
CAGCTGGAGT TTTCCGAATT TCCAGATCCG ATGTTGTGTT CAGATTATGT GCAGTTACTA 
AATTCCCCAC CTTGTTGTGA GCAAAAATGC AGCACTGTAT CATGGGAGGA GCTGAAATCC 
ATGGATTTAC CATCTTTTGA ACCTGCATTC TTGGTCCTCT GCCGAGTTCT GCTCAATGTT 
ATCCATGAAT GTCTAAAGCT AAGGTTGGAA CAAAGACCTG CTGGGGAGCC TTCTCTTTTG 
AGTATTAAAC AGCTAGTGAG AGAGTGTAAA GAGGTTCTGA AGGGTGGCCT GTTAATGAAG 
CAATATTACC AGTTTATGTT GCGGGAGGTG TTAGATGACT TACAAAAGAT TGATTGCAAC 
ATCGATTCTT TTGAAGAGGA CCTGCATAAA ATGTTAATGG TTTACTTTGA TTATATGCGA 
AGCTGGATCC AAATGCTACA GCAGTTACCT CAAGCGTCTC ATAGTTTAAA AAATCTGTTG 
GAGGAAGAAT GGAATTTCAC CAAAGAAATA ACTCCTTACA TAAGAGGAGG TGAAGCTCAA 
GCTGGAAAAC TATTCTGTGA TATTGCAGGA ATGCTGCTGA AATCTACAGG AATATTTTTA 
GATTCTGGAC TACAAGACAG TTGTGATGAA TTTTGGGCCA GTGCCGATGA CAGTACTGCA 
TCAGATGAAA TCAGGAGGTC TGTTATAGAG ACGAGCCGAG CACTCAAAGA ACTGTTTCAT 
GAAGCTAGAG AAAGGGCCTC CAAAGCTCTT GGTTTTGCTA AAATGCTGAG GAAGGATCTT 
GAAATAGCAG CAGAGTTTGC ATTGTCAGCC CCTGTGCGAG ATCTTCTGAA TGCTCTGAAA 
ACAAAAGAGT ATGTGAAGGT ACAAATCCCT GGACTCGAGA ATTTACAAGT ATTTGTACCA 
AATAGTATTG CAGGTGAGAA ATCTGTGATT TTGCAGCTCC TCAACGCAGC TGCTGGAAAG 
GATTGCTCAA AGGATTCGGA TGAAGTTGCG TATGAAGCCT ACTTACTGAT GACCAAACAC 
AGCGATAAAA CCCATGAACT GGATGACAGC TGGAGTTCGT GGGAGGGCCA GCCAGTCAAA 
GTAGTGCCAC AAGTAGAAAC TGTTGATACA CTGAGAACCA TGCAGGTTGA TAACCTTCTC 
CTTGTAGTCA TTCAATCTGC CCACCTTGTG AGTCAGAGGA AAGCTTTCCA GCAGTCTATC 
GAAGGGCTAA TTTCTCTGCA CCAGGAACAG ACATCCAGCC AACCGATCAT CGCCAAAGCT 
CTACAACAGC TAAAGAGTGA TGCATTACAG CTATGCAATA AGATAAGCAG TGCCATTGAC 
AGAGTTGATC ACATGTTCAC GGCTGAGTTT GATGCTGAAG TGGATGAATC TGAATCTGCC 
ACTTTGCAGC AATACTACCG GGAAGCCATG ATTCAGGGTT ATAATTTTGG TTTTGAGTAC 
CATAAAGAAG TTGTTCGTCT GATGTCTGGA GAATTCAGGC AGAAAATTGG AGACAAGTAT 
ATCAGCTTTG CCAGAAAATG GATGAATTAT GTGCTAACAA AATGTGAGAG CGGCCGAGGC 
ACTCGACCCA GATGGGCAAC TCAAGGTTTT GATTTTCTTC AAGCCATTGA GCCGGCATTT 
ATTTCAGCTC TCCCGGAAGA TGACTTCTTG AGTTTACAAG CTCTGATGAA TGAGTGCATC 
GGCCATGTTA TTGGAAAACC TCACAGTCCT GTTACAGGTT TGTACCTTGC TATTCATCGA 
AACAGTCCCC GTCCTGTAAA AGTACCTCGG TGCCATAGTG ATCCACCAAA TCCTCATCTT 
ATCATCCCCA CGCCTGAAGG CTTCAGCACG CGGAGTGTCC CTTGCGATGC CCGGAGCCAT 
TGCAGCGTTG CTGTCAGTCG CCCCGCTCCC GTCGGTAGCG ACTCAGCTCA GCCCAAAGCT 
GCCAGCAGTG CAAATGACAC CAGGGGTTCC AGTGTCCCTG AAAATGATCG ACTGGCTTCA 
ATAGCAGCTG AGTTGCAGTT CAGATCCCTT AGTCGCCACT CCAGTCCCAC TGAAGAGCGA 
GAAGAACCAG CGTATCCAAA AGGAGATTCA AGTGGCTCGT CCCGGCGGAG TTGGGAGCTC 
AGAACTTTAA TCAACCAGAC CAAAGACACT GCTTCCAAGC AAGCACCCAT TGAAGCTGTT 
CAAAAGTCTG TTCAGTTGTT TGAAGAAAGG AGATACAGAG AGATGAGGAG GAAGAACATC 
ATTGGCCAAG TTTGTGACAC ACCGAAATCT TATGATAATG TCATGCATGT AGGTTTGAGA 
AAAGTGACTT TCAAGTGGCA GAGAGGAAAC AAAATTGGTG AAGGCCAGTA CGGGAAGGTC 
TATACCTGCA TCAGTGTTGA CACTGGAGAG CTGATGGCTA TGAAGGAAAT AAGATTTCAA 
CCTAATGATC ACAAAACCAT CAAAGAAACA GCCGATGAAC TGAAGATCTT TGAAGGCATC 
AAACACCCTA ATCTTGTTCG TTATTTTGGA GTGGAGCTCC ATAGGGAAGA AATGTACATC 
TTCATGGAGT ACTGTGATGA GGGCACTCTG GAGGAGGTAT CAAAACTGGG CCTTCAGGAG 
CATGTCATTA GGCTCTACTC AAAGCAAATC ACGGTTGCTA TCAATGTACT ACATGAACAT 
GGTATTGTTC ATCGAGACAT TAAAGGAGCC AACATCTTTC TTACCTCATC TGGACTGATT 
AAACTAGGAG ACTTTGGCTG CTCAGTGAAA CTGAAGAACA ACACCCAGAC AATGCCTGGA 
GAAGTGAACA GTACTTTGGG GACTGCAGCG TACATGGCTC CAGAAGTAAT TACCAGAGCT 
AAAGGAGAAG GGCACGGACG CGCAGCGGAC ATCTGGAGCC TGGGCTGTGT TGTTATAGAG 
ATGGTCACGG GCAAGAGGCC TTGGCACGAA TACGAGCACA ACTTCCAGAT CATGTATAAA 
GTGGGGATGG GTCATAAACC TCCAATTCCT GACAAAGTGA GCCCAGAAGG GAAGGACTTC 
CTTTGCCACT GCCTGGAAAG TGACCCAAAG ATGAGGTGGA CTGCCAGCCA GCTCCTCGAT 
CATCCTTTTG TCAAGGTTTG CACAGATGAA GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_014951006.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014806492.1 Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCa71666
Clone ID Related Accession (Same CDS sequence)	XM_014951006.1
Accession Version	XM_014951006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090922.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTTTGTACCT TGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAAGTACCT CGGTGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACGCCTGA AGGCTTCAGC ACGCGGAGTG TCCCTTGCGA TGCCCGGAGC 
CATTGCAGCG TTGCTGTCAG TCGCCCCGCT CCCGTCGGTA GCGACTCAGC TCAGCCCAAA 
GCTGCCAGCA GTGCAAATGA CACCAGGGGT TCCAGTGTCC CTGAAAATGA TCGACTGGCT 
TCAATAGCAG CTGAGTTGCA GTTCAGATCC CTTAGTCGCC ACTCCAGTCC CACTGAAGAG 
CGAGAAGAAC CAGCGTATCC AAAAGGAGAT TCAAGTGGCT CGTCCCGGCG GAGTTGGGAG 
CTCAGAACTT TAATCAACCA GACCAAAGGT GAAGGCCAGT ACGGGAAGGT CTATACCTGC 
ATCAGTGTTG ACACTGGAGA GCTGATGGCT ATGAAGGAAA TAAGATTTCA ACCTAATGAT 
CACAAAACCA TCAAAGAAAC AGCCGATGAA CTGAAGATCT TTGAAGGCAT CAAACACCCT 
AATCTTGTTC GTTATTTTGG AGTGGAGCTC CATAGGGAAG AAATGTACAT CTTCATGGAG 
TACTGTGATG AGGGCACTCT GGAGGAGGTA TCAAAACTGG GCCTTCAGGA GCATGTCATT 
AGGCTCTACT CAAAGCAAAT CACGGTTGCT ATCAATGTAC TACATGAACA TGGTATTGTT 
CATCGAGACA TTAAAGGAGC CAACATCTTT CTTACCTCAT CTGGACTGAT TAAACTAGGA 
GACTTTGGCT GCTCAGTGAA ACTGAAGAAC AACACCCAGA CAATGCCTGG AGAAGTGAAC 
AGTACTTTGG GGACTGCAGC GTACATGGCT CCAGAAGTAA TTACCAGAGC TAAAGGAGAA 
GGGCACGGAC GCGCAGCGGA CATCTGGAGC CTGGGCTGTG TTGTTATAGA GATGGTCACG 
GGCAAGAGGC CTTGGCACGA ATACGAGCAC AACTTCCAGA TCATGTATAA AGTGGGGATG 
GGTCATAAAC CTCCAATTCC TGACAAAGTG AGCCCAGAAG GGAAGGACTT CCTTTGCCAC 
TGCCTGGAAA GTGACCCAAA GATGAGGTGG ACTGCCAGCC AGCTCCTCGA TCATCCTTTT 
GTCAAGGTTT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014806492.1
CDS	842..5368
Translation	MRDVAVLVSAAAMEEPPPPGRKECKSEDATCKISESDPEELSDEASTDTFYGTSPPSTPRQMKRMSTKHQKNNIGRPASRSTLKEKMSVPTQSVHKDNGKTVENVEEHSYKQGKKIRDTLRTTERDHKKNVQCSFMLDSVGGSLPKKPIPDVDLNKPYLSLGCSNAKLPVSVPMPIPRTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSSVWLNRSNELIWLELQAWHAGRSINDQDLYLYAARQAIPDIINEILTFKVNYGSFSCVKNGASLNGTSEGVCKATHGTSALGYSSYHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKDNDPEDDEVDRETEVKESSGTCTEESDGDEQISEENVEELRQAIKNNFSNKPNFDFQDHFSRRLDRLESEDDSASWAIPECSAEASCSQHCLTSIYRPFVDKALKQMGLRKLILRLHKLMHGSLQRARMALVRSDHQLEFSEFPDPMLCSDYVQLLNSPPCCEQKCSTVSWEELKSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLREVLDDLQKIDCNIDSFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITPYIRGGEAQAGKLFCDIAGMLLKSTGIFLDSGLQDSCDEFWASADDSTASDEIRRSVIETSRALKELFHEARERASKALGFAKMLRKDLEIAAEFALSAPVRDLLNALKTKEYVKVQIPGLENLQVFVPNSIAGEKSVILQLLNAAAGKDCSKDSDEVAYEAYLLMTKHSDKTHELDDSWSSWEGQPVKVVPQVETVDTLRTMQVDNLLLVVIQSAHLVSQRKAFQQSIEGLISLHQEQTSSQPIIAKALQQLKSDALQLCNKISSAIDRVDHMFTAEFDAEVDESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPCDARSHCSVAVSRPAPVGSDSAQPKAASSANDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEEREEPAYPKGDSSGSSRRSWELRTLINQTKGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLGLQEHVIRLYSKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNTQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPDKVSPEGKDFLCHCLESDPKMRWTASQLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_014951006.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014951006.1

ORF Insert Sequence:

ATGAGAGACG TCGCCGTGCT GGTCTCTGCC GCCGCCATGG AGGAGCCGCC GCCGCCGGGG 
AGGAAAGAGT GCAAATCAGA AGATGCTACT TGCAAGATCT CTGAATCTGA CCCTGAGGAA 
TTATCAGATG AAGCAAGTAC TGACACTTTC TATGGAACTT CTCCTCCTAG TACACCCCGC 
CAGATGAAGC GTATGTCAAC AAAGCATCAG AAAAATAACA TTGGGAGACC TGCTAGCCGC 
TCCACTTTGA AAGAAAAAAT GAGTGTGCCA ACTCAGTCTG TGCATAAAGA CAACGGGAAA 
ACCGTTGAGA ACGTGGAGGA GCACAGCTAT AAGCAAGGAA AAAAGATCCG AGACACCCTA 
AGGACAACAG AACGAGATCA CAAGAAAAAT GTGCAGTGTT CATTTATGTT AGACTCAGTT 
GGTGGGTCTC TGCCAAAAAA ACCAATCCCA GATGTTGATC TCAATAAGCC TTACCTCAGC 
CTTGGCTGTA GCAATGCTAA GCTTCCAGTC TCTGTGCCCA TGCCAATACC CAGAACTGCT 
CGCCAGACTT CTAGGACTGA TTGCCCAGCA GACCGCTTAA AATTCTTTGA AACCCTGCGG 
CTTTTGTTAA AACTTACCTC AGTCTCAAAG AAAAAGGACA GGGAGCAAAG AGGGCAGGAA 
AACACCTCTT CTGTCTGGCT GAACCGATCC AATGAACTGA TCTGGCTGGA GCTACAAGCT 
TGGCATGCTG GCCGGAGCAT TAATGACCAG GACCTCTATC TCTATGCAGC CCGTCAAGCT 
ATCCCTGATA TCATCAATGA AATCCTCACC TTCAAAGTGA ACTATGGAAG CTTCTCCTGT 
GTAAAAAATG GAGCCAGTCT CAATGGTACT TCAGAAGGAG TTTGCAAAGC AACACATGGA 
ACTAGTGCTT TGGGTTACTC AAGTTACCAT GAACACCTCC AACGCCAGCG GGTCTCATTT 
GAGCAAGTGA AGCGGATAAT GGAGCTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG 
CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAGG ACTTCCAAGA CAGAGTGCAG 
GCACTCTGTC TATGGTTAAA TATTACAAAA GACTTAAATC AAAAACTAAG GATTATGGGA 
ACTGTATTGG GCATCAAAAA TCTTTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT 
CCTCGACCAT CTAAGGACAA TGATCCAGAA GATGATGAAG TTGATAGGGA AACAGAAGTA 
AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGTG ATGAACAAAT CTCTGAGGAG 
AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA GTAATAAGCC AAATTTTGAC 
TTTCAGGACC ATTTTTCAAG AAGGCTTGAT AGGCTTGAAT CTGAGGATGA CTCTGCCTCT 
TGGGCTATTC CAGAATGCAG TGCTGAGGCA AGCTGCAGCC AACACTGTTT GACCTCTATT 
TACAGGCCGT TTGTAGATAA AGCACTGAAG CAAATGGGGT TAAGGAAACT AATTTTAAGA 
CTGCACAAGC TAATGCATGG TTCATTGCAA AGGGCCCGCA TGGCGTTGGT AAGGAGTGAC 
CACCAGCTGG AGTTTTCCGA ATTTCCAGAT CCGATGTTGT GTTCAGATTA TGTGCAGTTA 
CTAAATTCCC CACCTTGTTG TGAGCAAAAA TGCAGCACTG TATCATGGGA GGAGCTGAAA 
TCCATGGATT TACCATCTTT TGAACCTGCA TTCTTGGTCC TCTGCCGAGT TCTGCTCAAT 
GTTATCCATG AATGTCTAAA GCTAAGGTTG GAACAAAGAC CTGCTGGGGA GCCTTCTCTT 
TTGAGTATTA AACAGCTAGT GAGAGAGTGT AAAGAGGTTC TGAAGGGTGG CCTGTTAATG 
AAGCAATATT ACCAGTTTAT GTTGCGGGAG GTGTTAGATG ACTTACAAAA GATTGATTGC 
AACATCGATT CTTTTGAAGA GGACCTGCAT AAAATGTTAA TGGTTTACTT TGATTATATG 
CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCGT CTCATAGTTT AAAAAATCTG 
TTGGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCTT ACATAAGAGG AGGTGAAGCT 
CAAGCTGGAA AACTATTCTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT 
TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT 
GCATCAGATG AAATCAGGAG GTCTGTTATA GAGACGAGCC GAGCACTCAA AGAACTGTTT 
CATGAAGCTA GAGAAAGGGC CTCCAAAGCT CTTGGTTTTG CTAAAATGCT GAGGAAGGAT 
CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTGC GAGATCTTCT GAATGCTCTG 
AAAACAAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTA 
CCAAATAGTA TTGCAGGTGA GAAATCTGTG ATTTTGCAGC TCCTCAACGC AGCTGCTGGA 
AAGGATTGCT CAAAGGATTC GGATGAAGTT GCGTATGAAG CCTACTTACT GATGACCAAA 
CACAGCGATA AAACCCATGA ACTGGATGAC AGCTGGAGTT CGTGGGAGGG CCAGCCAGTC 
AAAGTAGTGC CACAAGTAGA AACTGTTGAT ACACTGAGAA CCATGCAGGT TGATAACCTT 
CTCCTTGTAG TCATTCAATC TGCCCACCTT GTGAGTCAGA GGAAAGCTTT CCAGCAGTCT 
ATCGAAGGGC TAATTTCTCT GCACCAGGAA CAGACATCCA GCCAACCGAT CATCGCCAAA 
GCTCTACAAC AGCTAAAGAG TGATGCATTA CAGCTATGCA ATAAGATAAG CAGTGCCATT 
GACAGAGTTG ATCACATGTT CACGGCTGAG TTTGATGCTG AAGTGGATGA ATCTGAATCT 
GCCACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG GTTATAATTT TGGTTTTGAG 
TACCATAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAG 
TATATCAGCT TTGCCAGAAA ATGGATGAAT TATGTGCTAA CAAAATGTGA GAGCGGCCGA 
GGCACTCGAC CCAGATGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAGCCGGCA 
TTTATTTCAG CTCTCCCGGA AGATGACTTC TTGAGTTTAC AAGCTCTGAT GAATGAGTGC 
ATCGGCCATG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTTTGTACCT TGCTATTCAT 
CGAAACAGTC CCCGTCCTGT AAAAGTACCT CGGTGCCATA GTGATCCACC AAATCCTCAT 
CTTATCATCC CCACGCCTGA AGGCTTCAGC ACGCGGAGTG TCCCTTGCGA TGCCCGGAGC 
CATTGCAGCG TTGCTGTCAG TCGCCCCGCT CCCGTCGGTA GCGACTCAGC TCAGCCCAAA 
GCTGCCAGCA GTGCAAATGA CACCAGGGGT TCCAGTGTCC CTGAAAATGA TCGACTGGCT 
TCAATAGCAG CTGAGTTGCA GTTCAGATCC CTTAGTCGCC ACTCCAGTCC CACTGAAGAG 
CGAGAAGAAC CAGCGTATCC AAAAGGAGAT TCAAGTGGCT CGTCCCGGCG GAGTTGGGAG 
CTCAGAACTT TAATCAACCA GACCAAAGGT GAAGGCCAGT ACGGGAAGGT CTATACCTGC 
ATCAGTGTTG ACACTGGAGA GCTGATGGCT ATGAAGGAAA TAAGATTTCA ACCTAATGAT 
CACAAAACCA TCAAAGAAAC AGCCGATGAA CTGAAGATCT TTGAAGGCAT CAAACACCCT 
AATCTTGTTC GTTATTTTGG AGTGGAGCTC CATAGGGAAG AAATGTACAT CTTCATGGAG 
TACTGTGATG AGGGCACTCT GGAGGAGGTA TCAAAACTGG GCCTTCAGGA GCATGTCATT 
AGGCTCTACT CAAAGCAAAT CACGGTTGCT ATCAATGTAC TACATGAACA TGGTATTGTT 
CATCGAGACA TTAAAGGAGC CAACATCTTT CTTACCTCAT CTGGACTGAT TAAACTAGGA 
GACTTTGGCT GCTCAGTGAA ACTGAAGAAC AACACCCAGA CAATGCCTGG AGAAGTGAAC 
AGTACTTTGG GGACTGCAGC GTACATGGCT CCAGAAGTAA TTACCAGAGC TAAAGGAGAA 
GGGCACGGAC GCGCAGCGGA CATCTGGAGC CTGGGCTGTG TTGTTATAGA GATGGTCACG 
GGCAAGAGGC CTTGGCACGA ATACGAGCAC AACTTCCAGA TCATGTATAA AGTGGGGATG 
GGTCATAAAC CTCCAATTCC TGACAAAGTG AGCCCAGAAG GGAAGGACTT CCTTTGCCAC 
TGCCTGGAAA GTGACCCAAA GATGAGGTGG ACTGCCAGCC AGCTCCTCGA TCATCCTTTT 
GTCAAGGTTT GCACAGATGA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MAP3K4 cDNA ORF clone, Calidris pugnax(ruff)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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