BLM cDNA ORF clone, Calidris pugnax(ruff)

The following BLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa72896 XM_014952774.1
Latest version!
Calidris pugnax Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCa72897 XM_014952775.1
Latest version!
Calidris pugnax Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCa72898 XM_014952776.1
Latest version!
Calidris pugnax Bloom syndrome, RecQ helicase-like (BLM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa72896
    Clone ID Related Accession (Same CDS sequence) XM_014952774.1
    Accession Version XM_014952774.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product Bloom syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090969.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCCC TGCCGCAGAA CAACCTGCGG GAGCAGCTGC GGCTCCACTC GGCCCGCGGA 
    GCCCTCAGCG CCCTCCGCAA CGCGCCCCCG CCGCGCCAGC GCTCGGCGGG CTTCACCTTC
    AAGAAGACCT CTCCGGCCGG CGGCCCGCCC CGGGGCCTCC CCGCCGCCTC CGCCTTGAGG
    GAGAAGGATG TTAACACCTC CCTGGCTGCG CCGGCCTCCC CCCTCCCTGC CTCCAAGGAC
    AAGAAAACGC AAATCCAGGC GTTTTTTCCC GTGGTGTCCG GTGGGTGCAG CCTCCAGCCC
    AGCCCGGTGC TCGCCAGGCC TCAGGAGGCG TCAAGAGGCC CTGTGGCCTC TGGGCAGAGG
    AGCGTGAGTG AGGGGCTGCG AGGGACCCCC GCGCCCCGTC CCGTGGCTGT CCCCGCCGTC
    GGGGATGAGT GGGACGACAT TGATGACTTT GATTTGTCGG GAATCGAGAA GAAGTACTGC
    AGGCCGCCCG TCCTGTCCCC CAAGGGGCAG CGGGCCACCT GCAAGGCTTC CCAGAGGCCC
    GAGCCCCGCC GGGATGAGCT GCCCGGCTCT TCCCCCGGTG CTGTGGGCCA GGACACGGGG
    CCTGGCAGCC GTGGCACCCC GGGGCGTGGG GAGACCCCTC CAGAGGCCGA GCAACAACCC
    TCCTCGCAGC AGTCTGTGAT CTGCCTTGAG GATTCGGCTC CCTGCGGCGA TGAGAAGGCC
    GTGGGTGAGG ACCTTTGGGA GGATTTATCC GCTGACGTGA TTTTGGGTGA TGACAGGGAA
    GAGGCTCACC CAGGGTCTGG CGCTGGCGGC GGGGCCGGGC AGCGGGCGGG TGGCCAGGGG
    CAGAGCAGCC CCCTGGAACT GGACGCCGTC GACATCGAGC CCTTCCCTGA GCTCGAGGAG
    GACGATTACT TGGATGTCGT TCCACCATCC CCTGAGGAAG AGCTGCCTTC CTTCTTGCCT
    TCTGGAAAAA GCACTAGTAG TGTTTTCAAA GAGTCTCCTG CCAGTGGGAG GTCCACAAGC
    AGCTCTCAGT CCACGCCTGG GCCGGGTGCC ACGGCGCAGC CTGTTGCTGA CAGTGACCCT
    GGTGCAGAGG GTGCAGATAA GGACCTGCCT TTGGAGCAGC AGCTCTTCGG CGTCATGGCC
    ACCATCTGTG AGCTGGTGGA CGCCATCCCG CTCCGGGAGC TGCAGGGCCT CTCCTGCGCC
    AAGGCCCTGC TCCAGCAGCG AGACCTCAGG AGCAGATTGC TGGCTGGTTC TGTCACTTTG
    AACAAAAGCG CCATGACCAA GGGTTTTCCT GGTTCCTGGA AGGGGTGTGT GGAGCAGAGC
    TCTCCTGCCC CTCCTGCTCC GGGCTCTGGC CCAACCTGGA GCTTCAGTTT GGGCAGGAAC
    TCGCCCCAGT CCCCAAACCT CCCCTCTGCC CTTTCCGGGA CTGTTCCTTC AAGCCATTTT
    TCTACCAAGA AAAATCAAAC CTTGGACACC CCTTGTGCTT CAAAGCCAAG TACCGAGGAG
    GTTACCTGCC CAGGAACTGC AGCACCATCC TCCTCCCGGG GACACGGCGG GGACAGAGCT
    TCCCTCAGCC CTCACCCTGA GACCTCCTTC CACAGCAGCT CCTGCGGGGA GCCGGGGCTG
    AGGAACGGGG AGAGCTGTCT CCTGCTGGAG AGGCCTCTCA CCAGCACCGC TCTGCGAGAC
    CAGGGCAGGG CTCCCACCAG CAGCTCTGAG CAGGACGCTC TGGGGCTGAA CCACACGGAC
    TTTGACCTCG ATCACTTTGA TATCGACGAT TTTGATGAGG ACTGGGAGAA TTCGGTGAAT
    GTTTCGGTAC CCGAGACGCC GTCGACGCCG CTGTATCAGC CCGTCAGGGA GGGGCCTCCT
    GCCAGATCGC TCTCCTCAAA GATCCTGTCC AGAGCCAAAG TCTCTGCTGC TGCATCAAAC
    CCTCCTGCTC CAAAATCCAG CTTCTTGATG GCAACAAAGA ACCACTCGGA CCCGCCGGCC
    ACTAACCCTG CGCTGGACCG CTTCAGGGGC ACGAAGTTCT CCCATTCTGA AGGGATGATG
    AATATATTTC ACAAGAAGTT TGGTTTGCAC TATTTTCGGA CAAACCAGCT GGAGGCCATC
    AACGCTGCTC TGCTGGGTGA AGACTGTTTC ATCCTGATGC CCACGGGGGG TGGGAAAAGC
    TTGTGCTACC AGTTACCGGC CTGTGTCTCT GCTGGAGTCA CCATCGTCAT CTCCCCACTG
    AGGTCCCTGA TCATAGACCA AGTGCAGAAA CTGAAGACTT TAGATATTGC TGCAACATAT
    CTGACTGGCG ATAGAACAGA TGCTGATGCT TCAAAAATAT ACATGCAACT GTCAAAAAAA
    GATCCTGTAA TAAAGCTTCT GTATGTTACC CCTGAGAAGG TTTGTGCCAG CAACCGCCTG
    ATGTCAGCCC TGGAAAATCT CTACGACAGG AAACTCCTGG AGCGTTTTGT CATCGACGAG
    GCCCACTGCG TCAGCCAGTG GGGTCACGAC TTCCGACAAG ACTACAAGCG GCTGAACATG
    CTGCGCAGGA GGTTCCGCTC CGTGCCCATG ATGGCTCTCA CGGCCACCGC CAACCCCAGG
    GTCCAGAAGG ACATTCAGAA CCAGCTGGAG ATGCTCAAAC CACAAGTGTT CACCATGAGC
    TTCAACAGGC ACAACTTGAA ATATGACGTG TTGCCCAAGA AGCCAAAGAA GGTGGCAATG
    GATTGCTTGG AATGGATTAA GAAGTATCAC CCACATGACT CTGGAATCAT TTATTGCCTT
    TCCCGACATG AATGTGACAC GACAGCAGCT ATCCTGCAGA AGGAAGGCCT TGCTGCACTG
    GCCTATCACG CTGGTCTCAC CGACTCCAAC AGGGATCTGG TACAGAAGAA GTGGATTAAC
    CAAGAAGGGT GTCAGGTGAT TTGTGCCACG ATTGCCTTTG GAATGGGAAT CGACAAGCCC
    GACGTGCGCT ACGTTATCCA CGCCTCCCTG CCCAAGTCTG TGGAAGGGTA CTACCAGGAG
    TCGGGCAGAG CGGGGCGGGA CGGGGAGATG TCTCACTGCC TGCTCTTCTA CAGCTACAGC
    GACGTCACCC GGCTCCGCAG GCTGATACTC ATGGAGAAGG ATGGGAACAG CCACACGAGG
    CAGACCCACT TTAACAACCT GTACAGTATG GTTCACTACT GTGAGAATTT GGTCGACTGC
    CGGAGAATCC AGCTTTTGGC CTACTTTGGG GAGACTAATT TCAATCCCAC CTTCTGTAAA
    GATCACCCAG AGGTTACTTG CGACAACTGC AGTAGAAAGA AGGATTACAA ATCACGAGAC
    GTAACGGATG ACGTGAAGAG CATTGTGAGA TTTGTACAAG AGCACTGTGG ACAAACGGGA
    CGAACAAATG CAAAGAGAAA TGCAGGTTTT GGAAGATACA CGCTGAATAT GATGGTAGAC
    ATCTTCTTAG GTGCCAAGAG CGCGAAGGTT CAGTCTGGGA TATTTGGGAA GGGAGCTGCC
    TACTCGCGGC ACAACGCCGA AAGGCTCTTC AGAAAACTGG TCTTGGACAA GATTCTAGAT
    GAAGACTTGT ACATCACTGC TAACGACCAG GCTGTGGCAT ATGTCATCCT GGGACAGAAG
    GCTCAGGCGG TGCTCAACGG GTCCCTGCAG GTGGAGTTTC ATGAAACGGA AAGCGCCAGC
    GCCGTCAGAA AGCAAAGGGC TTCTGTGGCA AAGGTGTCCC AGCGGGAAGA GATGGTTAAA
    AAGTGCCTTG GGGAACTCAC GGACACCTGC AAAACGCTTG GGAAAGTCTT TGACGTGCAT
    TACTTCAATA TTTTCAGTAC CTCAACACTA AAGAAAATAG CAGAGACCTT GTCATCTGAT
    GTGGAAGTTT TACTGCAGAT TGATGGTGTC ACAGAAGATA AACTGGAAAA ATACGGAGCA
    GAAATAATTA AAGTGATGGA TAAGTACTCG GAGTGGAGCG TACCAGAAGA CACTGTTGGC
    CAAAGCGTGG ACACGGCCGC GGGAAGCACG GGCACACCCG AGAGCGAGGG AGAAGCCGAA
    GACGTCGTTA CCACCTCAAG CTATTTCTGC AATAACGCAA ACCAAAGGAG GAAAAGGAAA
    AGGCCACCAA ACTTCAGAGA CTCGAAGAGG AAAAAGACAA GCGCTGGCGG TAGCCAGCAG
    TTCCATCCCA AAGGGGCCCA CAGCAGGTAC CGGCGGCCCC GACGCCAGCC CGGGCCCAGG
    GCTCCCGGCC CCTCGCTGCC CGTCACCGCC TCCCAGGGAG CCGCCGCCAA GCTGGGGATT
    ATGGCACCTC CCAAACCCAA AAACAGGCAG TTCCTTCAGC CTTCGTATTC AGTACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014808260.1
    CDS58..4377
    Translation

    Target ORF information:

    RefSeq Version XM_014952774.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014952774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCCC TGCCGCAGAA CAACCTGCGG GAGCAGCTGC GGCTCCACTC GGCCCGCGGA 
    GCCCTCAGCG CCCTCCGCAA CGCGCCCCCG CCGCGCCAGC GCTCGGCGGG CTTCACCTTC
    AAGAAGACCT CTCCGGCCGG CGGCCCGCCC CGGGGCCTCC CCGCCGCCTC CGCCTTGAGG
    GAGAAGGATG TTAACACCTC CCTGGCTGCG CCGGCCTCCC CCCTCCCTGC CTCCAAGGAC
    AAGAAAACGC AAATCCAGGC GTTTTTTCCC GTGGTGTCCG GTGGGTGCAG CCTCCAGCCC
    AGCCCGGTGC TCGCCAGGCC TCAGGAGGCG TCAAGAGGCC CTGTGGCCTC TGGGCAGAGG
    AGCGTGAGTG AGGGGCTGCG AGGGACCCCC GCGCCCCGTC CCGTGGCTGT CCCCGCCGTC
    GGGGATGAGT GGGACGACAT TGATGACTTT GATTTGTCGG GAATCGAGAA GAAGTACTGC
    AGGCCGCCCG TCCTGTCCCC CAAGGGGCAG CGGGCCACCT GCAAGGCTTC CCAGAGGCCC
    GAGCCCCGCC GGGATGAGCT GCCCGGCTCT TCCCCCGGTG CTGTGGGCCA GGACACGGGG
    CCTGGCAGCC GTGGCACCCC GGGGCGTGGG GAGACCCCTC CAGAGGCCGA GCAACAACCC
    TCCTCGCAGC AGTCTGTGAT CTGCCTTGAG GATTCGGCTC CCTGCGGCGA TGAGAAGGCC
    GTGGGTGAGG ACCTTTGGGA GGATTTATCC GCTGACGTGA TTTTGGGTGA TGACAGGGAA
    GAGGCTCACC CAGGGTCTGG CGCTGGCGGC GGGGCCGGGC AGCGGGCGGG TGGCCAGGGG
    CAGAGCAGCC CCCTGGAACT GGACGCCGTC GACATCGAGC CCTTCCCTGA GCTCGAGGAG
    GACGATTACT TGGATGTCGT TCCACCATCC CCTGAGGAAG AGCTGCCTTC CTTCTTGCCT
    TCTGGAAAAA GCACTAGTAG TGTTTTCAAA GAGTCTCCTG CCAGTGGGAG GTCCACAAGC
    AGCTCTCAGT CCACGCCTGG GCCGGGTGCC ACGGCGCAGC CTGTTGCTGA CAGTGACCCT
    GGTGCAGAGG GTGCAGATAA GGACCTGCCT TTGGAGCAGC AGCTCTTCGG CGTCATGGCC
    ACCATCTGTG AGCTGGTGGA CGCCATCCCG CTCCGGGAGC TGCAGGGCCT CTCCTGCGCC
    AAGGCCCTGC TCCAGCAGCG AGACCTCAGG AGCAGATTGC TGGCTGGTTC TGTCACTTTG
    AACAAAAGCG CCATGACCAA GGGTTTTCCT GGTTCCTGGA AGGGGTGTGT GGAGCAGAGC
    TCTCCTGCCC CTCCTGCTCC GGGCTCTGGC CCAACCTGGA GCTTCAGTTT GGGCAGGAAC
    TCGCCCCAGT CCCCAAACCT CCCCTCTGCC CTTTCCGGGA CTGTTCCTTC AAGCCATTTT
    TCTACCAAGA AAAATCAAAC CTTGGACACC CCTTGTGCTT CAAAGCCAAG TACCGAGGAG
    GTTACCTGCC CAGGAACTGC AGCACCATCC TCCTCCCGGG GACACGGCGG GGACAGAGCT
    TCCCTCAGCC CTCACCCTGA GACCTCCTTC CACAGCAGCT CCTGCGGGGA GCCGGGGCTG
    AGGAACGGGG AGAGCTGTCT CCTGCTGGAG AGGCCTCTCA CCAGCACCGC TCTGCGAGAC
    CAGGGCAGGG CTCCCACCAG CAGCTCTGAG CAGGACGCTC TGGGGCTGAA CCACACGGAC
    TTTGACCTCG ATCACTTTGA TATCGACGAT TTTGATGAGG ACTGGGAGAA TTCGGTGAAT
    GTTTCGGTAC CCGAGACGCC GTCGACGCCG CTGTATCAGC CCGTCAGGGA GGGGCCTCCT
    GCCAGATCGC TCTCCTCAAA GATCCTGTCC AGAGCCAAAG TCTCTGCTGC TGCATCAAAC
    CCTCCTGCTC CAAAATCCAG CTTCTTGATG GCAACAAAGA ACCACTCGGA CCCGCCGGCC
    ACTAACCCTG CGCTGGACCG CTTCAGGGGC ACGAAGTTCT CCCATTCTGA AGGGATGATG
    AATATATTTC ACAAGAAGTT TGGTTTGCAC TATTTTCGGA CAAACCAGCT GGAGGCCATC
    AACGCTGCTC TGCTGGGTGA AGACTGTTTC ATCCTGATGC CCACGGGGGG TGGGAAAAGC
    TTGTGCTACC AGTTACCGGC CTGTGTCTCT GCTGGAGTCA CCATCGTCAT CTCCCCACTG
    AGGTCCCTGA TCATAGACCA AGTGCAGAAA CTGAAGACTT TAGATATTGC TGCAACATAT
    CTGACTGGCG ATAGAACAGA TGCTGATGCT TCAAAAATAT ACATGCAACT GTCAAAAAAA
    GATCCTGTAA TAAAGCTTCT GTATGTTACC CCTGAGAAGG TTTGTGCCAG CAACCGCCTG
    ATGTCAGCCC TGGAAAATCT CTACGACAGG AAACTCCTGG AGCGTTTTGT CATCGACGAG
    GCCCACTGCG TCAGCCAGTG GGGTCACGAC TTCCGACAAG ACTACAAGCG GCTGAACATG
    CTGCGCAGGA GGTTCCGCTC CGTGCCCATG ATGGCTCTCA CGGCCACCGC CAACCCCAGG
    GTCCAGAAGG ACATTCAGAA CCAGCTGGAG ATGCTCAAAC CACAAGTGTT CACCATGAGC
    TTCAACAGGC ACAACTTGAA ATATGACGTG TTGCCCAAGA AGCCAAAGAA GGTGGCAATG
    GATTGCTTGG AATGGATTAA GAAGTATCAC CCACATGACT CTGGAATCAT TTATTGCCTT
    TCCCGACATG AATGTGACAC GACAGCAGCT ATCCTGCAGA AGGAAGGCCT TGCTGCACTG
    GCCTATCACG CTGGTCTCAC CGACTCCAAC AGGGATCTGG TACAGAAGAA GTGGATTAAC
    CAAGAAGGGT GTCAGGTGAT TTGTGCCACG ATTGCCTTTG GAATGGGAAT CGACAAGCCC
    GACGTGCGCT ACGTTATCCA CGCCTCCCTG CCCAAGTCTG TGGAAGGGTA CTACCAGGAG
    TCGGGCAGAG CGGGGCGGGA CGGGGAGATG TCTCACTGCC TGCTCTTCTA CAGCTACAGC
    GACGTCACCC GGCTCCGCAG GCTGATACTC ATGGAGAAGG ATGGGAACAG CCACACGAGG
    CAGACCCACT TTAACAACCT GTACAGTATG GTTCACTACT GTGAGAATTT GGTCGACTGC
    CGGAGAATCC AGCTTTTGGC CTACTTTGGG GAGACTAATT TCAATCCCAC CTTCTGTAAA
    GATCACCCAG AGGTTACTTG CGACAACTGC AGTAGAAAGA AGGATTACAA ATCACGAGAC
    GTAACGGATG ACGTGAAGAG CATTGTGAGA TTTGTACAAG AGCACTGTGG ACAAACGGGA
    CGAACAAATG CAAAGAGAAA TGCAGGTTTT GGAAGATACA CGCTGAATAT GATGGTAGAC
    ATCTTCTTAG GTGCCAAGAG CGCGAAGGTT CAGTCTGGGA TATTTGGGAA GGGAGCTGCC
    TACTCGCGGC ACAACGCCGA AAGGCTCTTC AGAAAACTGG TCTTGGACAA GATTCTAGAT
    GAAGACTTGT ACATCACTGC TAACGACCAG GCTGTGGCAT ATGTCATCCT GGGACAGAAG
    GCTCAGGCGG TGCTCAACGG GTCCCTGCAG GTGGAGTTTC ATGAAACGGA AAGCGCCAGC
    GCCGTCAGAA AGCAAAGGGC TTCTGTGGCA AAGGTGTCCC AGCGGGAAGA GATGGTTAAA
    AAGTGCCTTG GGGAACTCAC GGACACCTGC AAAACGCTTG GGAAAGTCTT TGACGTGCAT
    TACTTCAATA TTTTCAGTAC CTCAACACTA AAGAAAATAG CAGAGACCTT GTCATCTGAT
    GTGGAAGTTT TACTGCAGAT TGATGGTGTC ACAGAAGATA AACTGGAAAA ATACGGAGCA
    GAAATAATTA AAGTGATGGA TAAGTACTCG GAGTGGAGCG TACCAGAAGA CACTGTTGGC
    CAAAGCGTGG ACACGGCCGC GGGAAGCACG GGCACACCCG AGAGCGAGGG AGAAGCCGAA
    GACGTCGTTA CCACCTCAAG CTATTTCTGC AATAACGCAA ACCAAAGGAG GAAAAGGAAA
    AGGCCACCAA ACTTCAGAGA CTCGAAGAGG AAAAAGACAA GCGCTGGCGG TAGCCAGCAG
    TTCCATCCCA AAGGGGCCCA CAGCAGGTAC CGGCGGCCCC GACGCCAGCC CGGGCCCAGG
    GCTCCCGGCC CCTCGCTGCC CGTCACCGCC TCCCAGGGAG CCGCCGCCAA GCTGGGGATT
    ATGGCACCTC CCAAACCCAA AAACAGGCAG TTCCTTCAGC CTTCGTATTC AGTACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa72897
    Clone ID Related Accession (Same CDS sequence) XM_014952775.1
    Accession Version XM_014952775.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product Bloom syndrome protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090969.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCCC TGCCGCAGAA CAACCTGCGG GAGCAGCTGC GGCTCCACTC GGCCCGCGGA 
    GCCCTCAGCG CCCTCCGCAA CGCGCCCCCG CCGCGCCAGC GCTCGGCGGG CTTCACCTTC
    AAGAAGACCT CTCCGGCCGG CGGCCCGCCC CGGGGCCTCC CCGCCGCCTC CGCCTTGAGG
    GAGAAGGATG TTAACACCTC CCTGGCTGCG CCGGCCTCCC CCCTCCCTGC CTCCAAGGAC
    AAGAAAACGC AAATCCAGGC GTTTTTTCCC GTGGTGTCCG GTGGGTGCAG CCTCCAGCCC
    AGCCCGGTGC TCGCCAGGCC TCAGGAGGCG TCAAGAGGCC CTGTGGCCTC TGGGCAGAGG
    AGCGTGAGTG AGGGGCTGCG AGGGACCCCC GCGCCCCGTC CCGTGGCTGT CCCCGCCGTC
    GGGGATGAGT GGGACGACAT TGATGACTTT GATTTGTCGG GAATCGAGAA GAAGTACTGC
    AGGCCGCCCG TCCTGTCCCC CAAGGGGCAG CGGGCCACCT GCAAGGCTTC CCAGAGGCCC
    GAGCCCCGCC GGGATGAGCT GCCCGGCTCT TCCCCCGGTG CTGTGGGCCA GGACACGGGG
    CCTGGCAGCC GTGGCACCCC GGGGCGTGGG GAGACCCCTC CAGAGGCCGA GCAACAACCC
    TCCTCGCAGC AGTCTGTGAT CTGCCTTGAG GATTCGGCTC CCTGCGGCGA TGAGAAGGCC
    GTGGGTGAGG ACCTTTGGGA GGATTTATCC GCTGACGTGA TTTTGGGTGA TGACAGGGAA
    GAGGCTCACC CAGGGTCTGG CGCTGGCGGC GGGGCCGGGC AGCGGGCGGG TGGCCAGGGG
    CAGAGCAGCC CCCTGGAACT GGACGCCGTC GACATCGAGC CCTTCCCTGA GCTCGAGGAG
    GACGATTACT TGGATGTCGT TCCACCATCC CCTGAGGAAG AGCTGCCTTC CTTCTTGCCT
    TCTGGAAAAA GCACTAGTGT TTTCAAAGAG TCTCCTGCCA GTGGGAGGTC CACAAGCAGC
    TCTCAGTCCA CGCCTGGGCC GGGTGCCACG GCGCAGCCTG TTGCTGACAG TGACCCTGGT
    GCAGAGGGTG CAGATAAGGA CCTGCCTTTG GAGCAGCAGC TCTTCGGCGT CATGGCCACC
    ATCTGTGAGC TGGTGGACGC CATCCCGCTC CGGGAGCTGC AGGGCCTCTC CTGCGCCAAG
    GCCCTGCTCC AGCAGCGAGA CCTCAGGAGC AGATTGCTGG CTGGTTCTGT CACTTTGAAC
    AAAAGCGCCA TGACCAAGGG TTTTCCTGGT TCCTGGAAGG GGTGTGTGGA GCAGAGCTCT
    CCTGCCCCTC CTGCTCCGGG CTCTGGCCCA ACCTGGAGCT TCAGTTTGGG CAGGAACTCG
    CCCCAGTCCC CAAACCTCCC CTCTGCCCTT TCCGGGACTG TTCCTTCAAG CCATTTTTCT
    ACCAAGAAAA ATCAAACCTT GGACACCCCT TGTGCTTCAA AGCCAAGTAC CGAGGAGGTT
    ACCTGCCCAG GAACTGCAGC ACCATCCTCC TCCCGGGGAC ACGGCGGGGA CAGAGCTTCC
    CTCAGCCCTC ACCCTGAGAC CTCCTTCCAC AGCAGCTCCT GCGGGGAGCC GGGGCTGAGG
    AACGGGGAGA GCTGTCTCCT GCTGGAGAGG CCTCTCACCA GCACCGCTCT GCGAGACCAG
    GGCAGGGCTC CCACCAGCAG CTCTGAGCAG GACGCTCTGG GGCTGAACCA CACGGACTTT
    GACCTCGATC ACTTTGATAT CGACGATTTT GATGAGGACT GGGAGAATTC GGTGAATGTT
    TCGGTACCCG AGACGCCGTC GACGCCGCTG TATCAGCCCG TCAGGGAGGG GCCTCCTGCC
    AGATCGCTCT CCTCAAAGAT CCTGTCCAGA GCCAAAGTCT CTGCTGCTGC ATCAAACCCT
    CCTGCTCCAA AATCCAGCTT CTTGATGGCA ACAAAGAACC ACTCGGACCC GCCGGCCACT
    AACCCTGCGC TGGACCGCTT CAGGGGCACG AAGTTCTCCC ATTCTGAAGG GATGATGAAT
    ATATTTCACA AGAAGTTTGG TTTGCACTAT TTTCGGACAA ACCAGCTGGA GGCCATCAAC
    GCTGCTCTGC TGGGTGAAGA CTGTTTCATC CTGATGCCCA CGGGGGGTGG GAAAAGCTTG
    TGCTACCAGT TACCGGCCTG TGTCTCTGCT GGAGTCACCA TCGTCATCTC CCCACTGAGG
    TCCCTGATCA TAGACCAAGT GCAGAAACTG AAGACTTTAG ATATTGCTGC AACATATCTG
    ACTGGCGATA GAACAGATGC TGATGCTTCA AAAATATACA TGCAACTGTC AAAAAAAGAT
    CCTGTAATAA AGCTTCTGTA TGTTACCCCT GAGAAGGTTT GTGCCAGCAA CCGCCTGATG
    TCAGCCCTGG AAAATCTCTA CGACAGGAAA CTCCTGGAGC GTTTTGTCAT CGACGAGGCC
    CACTGCGTCA GCCAGTGGGG TCACGACTTC CGACAAGACT ACAAGCGGCT GAACATGCTG
    CGCAGGAGGT TCCGCTCCGT GCCCATGATG GCTCTCACGG CCACCGCCAA CCCCAGGGTC
    CAGAAGGACA TTCAGAACCA GCTGGAGATG CTCAAACCAC AAGTGTTCAC CATGAGCTTC
    AACAGGCACA ACTTGAAATA TGACGTGTTG CCCAAGAAGC CAAAGAAGGT GGCAATGGAT
    TGCTTGGAAT GGATTAAGAA GTATCACCCA CATGACTCTG GAATCATTTA TTGCCTTTCC
    CGACATGAAT GTGACACGAC AGCAGCTATC CTGCAGAAGG AAGGCCTTGC TGCACTGGCC
    TATCACGCTG GTCTCACCGA CTCCAACAGG GATCTGGTAC AGAAGAAGTG GATTAACCAA
    GAAGGGTGTC AGGTGATTTG TGCCACGATT GCCTTTGGAA TGGGAATCGA CAAGCCCGAC
    GTGCGCTACG TTATCCACGC CTCCCTGCCC AAGTCTGTGG AAGGGTACTA CCAGGAGTCG
    GGCAGAGCGG GGCGGGACGG GGAGATGTCT CACTGCCTGC TCTTCTACAG CTACAGCGAC
    GTCACCCGGC TCCGCAGGCT GATACTCATG GAGAAGGATG GGAACAGCCA CACGAGGCAG
    ACCCACTTTA ACAACCTGTA CAGTATGGTT CACTACTGTG AGAATTTGGT CGACTGCCGG
    AGAATCCAGC TTTTGGCCTA CTTTGGGGAG ACTAATTTCA ATCCCACCTT CTGTAAAGAT
    CACCCAGAGG TTACTTGCGA CAACTGCAGT AGAAAGAAGG ATTACAAATC ACGAGACGTA
    ACGGATGACG TGAAGAGCAT TGTGAGATTT GTACAAGAGC ACTGTGGACA AACGGGACGA
    ACAAATGCAA AGAGAAATGC AGGTTTTGGA AGATACACGC TGAATATGAT GGTAGACATC
    TTCTTAGGTG CCAAGAGCGC GAAGGTTCAG TCTGGGATAT TTGGGAAGGG AGCTGCCTAC
    TCGCGGCACA ACGCCGAAAG GCTCTTCAGA AAACTGGTCT TGGACAAGAT TCTAGATGAA
    GACTTGTACA TCACTGCTAA CGACCAGGCT GTGGCATATG TCATCCTGGG ACAGAAGGCT
    CAGGCGGTGC TCAACGGGTC CCTGCAGGTG GAGTTTCATG AAACGGAAAG CGCCAGCGCC
    GTCAGAAAGC AAAGGGCTTC TGTGGCAAAG GTGTCCCAGC GGGAAGAGAT GGTTAAAAAG
    TGCCTTGGGG AACTCACGGA CACCTGCAAA ACGCTTGGGA AAGTCTTTGA CGTGCATTAC
    TTCAATATTT TCAGTACCTC AACACTAAAG AAAATAGCAG AGACCTTGTC ATCTGATGTG
    GAAGTTTTAC TGCAGATTGA TGGTGTCACA GAAGATAAAC TGGAAAAATA CGGAGCAGAA
    ATAATTAAAG TGATGGATAA GTACTCGGAG TGGAGCGTAC CAGAAGACAC TGTTGGCCAA
    AGCGTGGACA CGGCCGCGGG AAGCACGGGC ACACCCGAGA GCGAGGGAGA AGCCGAAGAC
    GTCGTTACCA CCTCAAGCTA TTTCTGCAAT AACGCAAACC AAAGGAGGAA AAGGAAAAGG
    CCACCAAACT TCAGAGACTC GAAGAGGAAA AAGACAAGCG CTGGCGGTAG CCAGCAGTTC
    CATCCCAAAG GGGCCCACAG CAGGTACCGG CGGCCCCGAC GCCAGCCCGG GCCCAGGGCT
    CCCGGCCCCT CGCTGCCCGT CACCGCCTCC CAGGGAGCCG CCGCCAAGCT GGGGATTATG
    GCACCTCCCA AACCCAAAAA CAGGCAGTTC CTTCAGCCTT CGTATTCAGT ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014808261.1
    CDS58..4374
    Translation

    Target ORF information:

    RefSeq Version XM_014952775.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014952775.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCCC TGCCGCAGAA CAACCTGCGG GAGCAGCTGC GGCTCCACTC GGCCCGCGGA 
    GCCCTCAGCG CCCTCCGCAA CGCGCCCCCG CCGCGCCAGC GCTCGGCGGG CTTCACCTTC
    AAGAAGACCT CTCCGGCCGG CGGCCCGCCC CGGGGCCTCC CCGCCGCCTC CGCCTTGAGG
    GAGAAGGATG TTAACACCTC CCTGGCTGCG CCGGCCTCCC CCCTCCCTGC CTCCAAGGAC
    AAGAAAACGC AAATCCAGGC GTTTTTTCCC GTGGTGTCCG GTGGGTGCAG CCTCCAGCCC
    AGCCCGGTGC TCGCCAGGCC TCAGGAGGCG TCAAGAGGCC CTGTGGCCTC TGGGCAGAGG
    AGCGTGAGTG AGGGGCTGCG AGGGACCCCC GCGCCCCGTC CCGTGGCTGT CCCCGCCGTC
    GGGGATGAGT GGGACGACAT TGATGACTTT GATTTGTCGG GAATCGAGAA GAAGTACTGC
    AGGCCGCCCG TCCTGTCCCC CAAGGGGCAG CGGGCCACCT GCAAGGCTTC CCAGAGGCCC
    GAGCCCCGCC GGGATGAGCT GCCCGGCTCT TCCCCCGGTG CTGTGGGCCA GGACACGGGG
    CCTGGCAGCC GTGGCACCCC GGGGCGTGGG GAGACCCCTC CAGAGGCCGA GCAACAACCC
    TCCTCGCAGC AGTCTGTGAT CTGCCTTGAG GATTCGGCTC CCTGCGGCGA TGAGAAGGCC
    GTGGGTGAGG ACCTTTGGGA GGATTTATCC GCTGACGTGA TTTTGGGTGA TGACAGGGAA
    GAGGCTCACC CAGGGTCTGG CGCTGGCGGC GGGGCCGGGC AGCGGGCGGG TGGCCAGGGG
    CAGAGCAGCC CCCTGGAACT GGACGCCGTC GACATCGAGC CCTTCCCTGA GCTCGAGGAG
    GACGATTACT TGGATGTCGT TCCACCATCC CCTGAGGAAG AGCTGCCTTC CTTCTTGCCT
    TCTGGAAAAA GCACTAGTGT TTTCAAAGAG TCTCCTGCCA GTGGGAGGTC CACAAGCAGC
    TCTCAGTCCA CGCCTGGGCC GGGTGCCACG GCGCAGCCTG TTGCTGACAG TGACCCTGGT
    GCAGAGGGTG CAGATAAGGA CCTGCCTTTG GAGCAGCAGC TCTTCGGCGT CATGGCCACC
    ATCTGTGAGC TGGTGGACGC CATCCCGCTC CGGGAGCTGC AGGGCCTCTC CTGCGCCAAG
    GCCCTGCTCC AGCAGCGAGA CCTCAGGAGC AGATTGCTGG CTGGTTCTGT CACTTTGAAC
    AAAAGCGCCA TGACCAAGGG TTTTCCTGGT TCCTGGAAGG GGTGTGTGGA GCAGAGCTCT
    CCTGCCCCTC CTGCTCCGGG CTCTGGCCCA ACCTGGAGCT TCAGTTTGGG CAGGAACTCG
    CCCCAGTCCC CAAACCTCCC CTCTGCCCTT TCCGGGACTG TTCCTTCAAG CCATTTTTCT
    ACCAAGAAAA ATCAAACCTT GGACACCCCT TGTGCTTCAA AGCCAAGTAC CGAGGAGGTT
    ACCTGCCCAG GAACTGCAGC ACCATCCTCC TCCCGGGGAC ACGGCGGGGA CAGAGCTTCC
    CTCAGCCCTC ACCCTGAGAC CTCCTTCCAC AGCAGCTCCT GCGGGGAGCC GGGGCTGAGG
    AACGGGGAGA GCTGTCTCCT GCTGGAGAGG CCTCTCACCA GCACCGCTCT GCGAGACCAG
    GGCAGGGCTC CCACCAGCAG CTCTGAGCAG GACGCTCTGG GGCTGAACCA CACGGACTTT
    GACCTCGATC ACTTTGATAT CGACGATTTT GATGAGGACT GGGAGAATTC GGTGAATGTT
    TCGGTACCCG AGACGCCGTC GACGCCGCTG TATCAGCCCG TCAGGGAGGG GCCTCCTGCC
    AGATCGCTCT CCTCAAAGAT CCTGTCCAGA GCCAAAGTCT CTGCTGCTGC ATCAAACCCT
    CCTGCTCCAA AATCCAGCTT CTTGATGGCA ACAAAGAACC ACTCGGACCC GCCGGCCACT
    AACCCTGCGC TGGACCGCTT CAGGGGCACG AAGTTCTCCC ATTCTGAAGG GATGATGAAT
    ATATTTCACA AGAAGTTTGG TTTGCACTAT TTTCGGACAA ACCAGCTGGA GGCCATCAAC
    GCTGCTCTGC TGGGTGAAGA CTGTTTCATC CTGATGCCCA CGGGGGGTGG GAAAAGCTTG
    TGCTACCAGT TACCGGCCTG TGTCTCTGCT GGAGTCACCA TCGTCATCTC CCCACTGAGG
    TCCCTGATCA TAGACCAAGT GCAGAAACTG AAGACTTTAG ATATTGCTGC AACATATCTG
    ACTGGCGATA GAACAGATGC TGATGCTTCA AAAATATACA TGCAACTGTC AAAAAAAGAT
    CCTGTAATAA AGCTTCTGTA TGTTACCCCT GAGAAGGTTT GTGCCAGCAA CCGCCTGATG
    TCAGCCCTGG AAAATCTCTA CGACAGGAAA CTCCTGGAGC GTTTTGTCAT CGACGAGGCC
    CACTGCGTCA GCCAGTGGGG TCACGACTTC CGACAAGACT ACAAGCGGCT GAACATGCTG
    CGCAGGAGGT TCCGCTCCGT GCCCATGATG GCTCTCACGG CCACCGCCAA CCCCAGGGTC
    CAGAAGGACA TTCAGAACCA GCTGGAGATG CTCAAACCAC AAGTGTTCAC CATGAGCTTC
    AACAGGCACA ACTTGAAATA TGACGTGTTG CCCAAGAAGC CAAAGAAGGT GGCAATGGAT
    TGCTTGGAAT GGATTAAGAA GTATCACCCA CATGACTCTG GAATCATTTA TTGCCTTTCC
    CGACATGAAT GTGACACGAC AGCAGCTATC CTGCAGAAGG AAGGCCTTGC TGCACTGGCC
    TATCACGCTG GTCTCACCGA CTCCAACAGG GATCTGGTAC AGAAGAAGTG GATTAACCAA
    GAAGGGTGTC AGGTGATTTG TGCCACGATT GCCTTTGGAA TGGGAATCGA CAAGCCCGAC
    GTGCGCTACG TTATCCACGC CTCCCTGCCC AAGTCTGTGG AAGGGTACTA CCAGGAGTCG
    GGCAGAGCGG GGCGGGACGG GGAGATGTCT CACTGCCTGC TCTTCTACAG CTACAGCGAC
    GTCACCCGGC TCCGCAGGCT GATACTCATG GAGAAGGATG GGAACAGCCA CACGAGGCAG
    ACCCACTTTA ACAACCTGTA CAGTATGGTT CACTACTGTG AGAATTTGGT CGACTGCCGG
    AGAATCCAGC TTTTGGCCTA CTTTGGGGAG ACTAATTTCA ATCCCACCTT CTGTAAAGAT
    CACCCAGAGG TTACTTGCGA CAACTGCAGT AGAAAGAAGG ATTACAAATC ACGAGACGTA
    ACGGATGACG TGAAGAGCAT TGTGAGATTT GTACAAGAGC ACTGTGGACA AACGGGACGA
    ACAAATGCAA AGAGAAATGC AGGTTTTGGA AGATACACGC TGAATATGAT GGTAGACATC
    TTCTTAGGTG CCAAGAGCGC GAAGGTTCAG TCTGGGATAT TTGGGAAGGG AGCTGCCTAC
    TCGCGGCACA ACGCCGAAAG GCTCTTCAGA AAACTGGTCT TGGACAAGAT TCTAGATGAA
    GACTTGTACA TCACTGCTAA CGACCAGGCT GTGGCATATG TCATCCTGGG ACAGAAGGCT
    CAGGCGGTGC TCAACGGGTC CCTGCAGGTG GAGTTTCATG AAACGGAAAG CGCCAGCGCC
    GTCAGAAAGC AAAGGGCTTC TGTGGCAAAG GTGTCCCAGC GGGAAGAGAT GGTTAAAAAG
    TGCCTTGGGG AACTCACGGA CACCTGCAAA ACGCTTGGGA AAGTCTTTGA CGTGCATTAC
    TTCAATATTT TCAGTACCTC AACACTAAAG AAAATAGCAG AGACCTTGTC ATCTGATGTG
    GAAGTTTTAC TGCAGATTGA TGGTGTCACA GAAGATAAAC TGGAAAAATA CGGAGCAGAA
    ATAATTAAAG TGATGGATAA GTACTCGGAG TGGAGCGTAC CAGAAGACAC TGTTGGCCAA
    AGCGTGGACA CGGCCGCGGG AAGCACGGGC ACACCCGAGA GCGAGGGAGA AGCCGAAGAC
    GTCGTTACCA CCTCAAGCTA TTTCTGCAAT AACGCAAACC AAAGGAGGAA AAGGAAAAGG
    CCACCAAACT TCAGAGACTC GAAGAGGAAA AAGACAAGCG CTGGCGGTAG CCAGCAGTTC
    CATCCCAAAG GGGCCCACAG CAGGTACCGG CGGCCCCGAC GCCAGCCCGG GCCCAGGGCT
    CCCGGCCCCT CGCTGCCCGT CACCGCCTCC CAGGGAGCCG CCGCCAAGCT GGGGATTATG
    GCACCTCCCA AACCCAAAAA CAGGCAGTTC CTTCAGCCTT CGTATTCAGT ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa72898
    Clone ID Related Accession (Same CDS sequence) XM_014952776.1
    Accession Version XM_014952776.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product Bloom syndrome protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090969.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGGCCC TGCCGCAGAA CAACCTGCGG GAGCAGCTGC GGCTCCACTC GGCCCGCGGA 
    GCCCTCAGCG CCCTCCGCAA CGCGCCCCCG CCGCGCCAGC GCTCGGCGGG CTTCACCTTC
    AAGAAGACCT CTCCGGCCGG CGGCCCGCCC CGGGGCCTCC CCGCCGCCTC CGCCTTGAGG
    GAGAAGGATG TTAACACCTC CCTGGCTGCG CCGGCCTCCC CCCTCCCTGC CTCCAAGGAC
    AAGAAAACGC AAATCCAGGC GTTTTTTCCC GTGGTGTCCG GTGGGTGCAG CCTCCAGCCC
    AGCCCGGTGC TCGCCAGGCC TCAGGAGGCG TCAAGAGGCC CTGTGGCCTC TGGGCAGAGG
    AGCGTGAGTG AGGGGCTGCG AGGGACCCCC GCGCCCCGTC CCGTGGCTGT CCCCGCCGTC
    GGGGATGAGT GGGACGACAT TGATGACTTT GATTTGTCGG GAATCGAGAA GAAGTACTGC
    AGGCCGCCCG TCCTGTCCCC CAAGGGGCAG CGGGCCACCT GCAAGGCTTC CCAGAGGCCC
    GAGCCCCGCC GGGATGAGCT GCCCGGCTCT TCCCCCGGTG CTGTGGGCCA GGACACGGGG
    CCTGGCAGCC GTGGCACCCC GGGGCGTGGG GAGACCCCTC CAGAGGCCGA GCAACAACCC
    TCCTCGCAGC AGTCTGTGAT CTGCCTTGAG GATTCGGCTC CCTGCGGCGA TGAGAAGGCC
    GTGGGGTCTG GCGCTGGCGG CGGGGCCGGG CAGCGGGCGG GTGGCCAGGG GCAGAGCAGC
    CCCCTGGAAC TGGACGCCGT CGACATCGAG CCCTTCCCTG AGCTCGAGGA GGACGATTAC
    TTGGATGTCG TTCCACCATC CCCTGAGGAA GAGCTGCCTT CCTTCTTGCC TTCTGGAAAA
    AGCACTAGTA GTGTTTTCAA AGAGTCTCCT GCCAGTGGGA GGTCCACAAG CAGCTCTCAG
    TCCACGCCTG GGCCGGGTGC CACGGCGCAG CCTGTTGCTG ACAGTGACCC TGGTGCAGAG
    GGTGCAGATA AGGACCTGCC TTTGGAGCAG CAGCTCTTCG GCGTCATGGC CACCATCTGT
    GAGCTGGTGG ACGCCATCCC GCTCCGGGAG CTGCAGGGCC TCTCCTGCGC CAAGGCCCTG
    CTCCAGCAGC GAGACCTCAG GAGCAGATTG CTGGCTGGTT CTGTCACTTT GAACAAAAGC
    GCCATGACCA AGGGTTTTCC TGGTTCCTGG AAGGGGTGTG TGGAGCAGAG CTCTCCTGCC
    CCTCCTGCTC CGGGCTCTGG CCCAACCTGG AGCTTCAGTT TGGGCAGGAA CTCGCCCCAG
    TCCCCAAACC TCCCCTCTGC CCTTTCCGGG ACTGTTCCTT CAAGCCATTT TTCTACCAAG
    AAAAATCAAA CCTTGGACAC CCCTTGTGCT TCAAAGCCAA GTACCGAGGA GGTTACCTGC
    CCAGGAACTG CAGCACCATC CTCCTCCCGG GGACACGGCG GGGACAGAGC TTCCCTCAGC
    CCTCACCCTG AGACCTCCTT CCACAGCAGC TCCTGCGGGG AGCCGGGGCT GAGGAACGGG
    GAGAGCTGTC TCCTGCTGGA GAGGCCTCTC ACCAGCACCG CTCTGCGAGA CCAGGGCAGG
    GCTCCCACCA GCAGCTCTGA GCAGGACGCT CTGGGGCTGA ACCACACGGA CTTTGACCTC
    GATCACTTTG ATATCGACGA TTTTGATGAG GACTGGGAGA ATTCGGTGAA TGTTTCGGTA
    CCCGAGACGC CGTCGACGCC GCTGTATCAG CCCGTCAGGG AGGGGCCTCC TGCCAGATCG
    CTCTCCTCAA AGATCCTGTC CAGAGCCAAA GTCTCTGCTG CTGCATCAAA CCCTCCTGCT
    CCAAAATCCA GCTTCTTGAT GGCAACAAAG AACCACTCGG ACCCGCCGGC CACTAACCCT
    GCGCTGGACC GCTTCAGGGG CACGAAGTTC TCCCATTCTG AAGGGATGAT GAATATATTT
    CACAAGAAGT TTGGTTTGCA CTATTTTCGG ACAAACCAGC TGGAGGCCAT CAACGCTGCT
    CTGCTGGGTG AAGACTGTTT CATCCTGATG CCCACGGGGG GTGGGAAAAG CTTGTGCTAC
    CAGTTACCGG CCTGTGTCTC TGCTGGAGTC ACCATCGTCA TCTCCCCACT GAGGTCCCTG
    ATCATAGACC AAGTGCAGAA ACTGAAGACT TTAGATATTG CTGCAACATA TCTGACTGGC
    GATAGAACAG ATGCTGATGC TTCAAAAATA TACATGCAAC TGTCAAAAAA AGATCCTGTA
    ATAAAGCTTC TGTATGTTAC CCCTGAGAAG GTTTGTGCCA GCAACCGCCT GATGTCAGCC
    CTGGAAAATC TCTACGACAG GAAACTCCTG GAGCGTTTTG TCATCGACGA GGCCCACTGC
    GTCAGCCAGT GGGGTCACGA CTTCCGACAA GACTACAAGC GGCTGAACAT GCTGCGCAGG
    AGGTTCCGCT CCGTGCCCAT GATGGCTCTC ACGGCCACCG CCAACCCCAG GGTCCAGAAG
    GACATTCAGA ACCAGCTGGA GATGCTCAAA CCACAAGTGT TCACCATGAG CTTCAACAGG
    CACAACTTGA AATATGACGT GTTGCCCAAG AAGCCAAAGA AGGTGGCAAT GGATTGCTTG
    GAATGGATTA AGAAGTATCA CCCACATGAC TCTGGAATCA TTTATTGCCT TTCCCGACAT
    GAATGTGACA CGACAGCAGC TATCCTGCAG AAGGAAGGCC TTGCTGCACT GGCCTATCAC
    GCTGGTCTCA CCGACTCCAA CAGGGATCTG GTACAGAAGA AGTGGATTAA CCAAGAAGGG
    TGTCAGGTGA TTTGTGCCAC GATTGCCTTT GGAATGGGAA TCGACAAGCC CGACGTGCGC
    TACGTTATCC ACGCCTCCCT GCCCAAGTCT GTGGAAGGGT ACTACCAGGA GTCGGGCAGA
    GCGGGGCGGG ACGGGGAGAT GTCTCACTGC CTGCTCTTCT ACAGCTACAG CGACGTCACC
    CGGCTCCGCA GGCTGATACT CATGGAGAAG GATGGGAACA GCCACACGAG GCAGACCCAC
    TTTAACAACC TGTACAGTAT GGTTCACTAC TGTGAGAATT TGGTCGACTG CCGGAGAATC
    CAGCTTTTGG CCTACTTTGG GGAGACTAAT TTCAATCCCA CCTTCTGTAA AGATCACCCA
    GAGGTTACTT GCGACAACTG CAGTAGAAAG AAGGATTACA AATCACGAGA CGTAACGGAT
    GACGTGAAGA GCATTGTGAG ATTTGTACAA GAGCACTGTG GACAAACGGG ACGAACAAAT
    GCAAAGAGAA ATGCAGGTTT TGGAAGATAC ACGCTGAATA TGATGGTAGA CATCTTCTTA
    GGTGCCAAGA GCGCGAAGGT TCAGTCTGGG ATATTTGGGA AGGGAGCTGC CTACTCGCGG
    CACAACGCCG AAAGGCTCTT CAGAAAACTG GTCTTGGACA AGATTCTAGA TGAAGACTTG
    TACATCACTG CTAACGACCA GGCTGTGGCA TATGTCATCC TGGGACAGAA GGCTCAGGCG
    GTGCTCAACG GGTCCCTGCA GGTGGAGTTT CATGAAACGG AAAGCGCCAG CGCCGTCAGA
    AAGCAAAGGG CTTCTGTGGC AAAGGTGTCC CAGCGGGAAG AGATGGTTAA AAAGTGCCTT
    GGGGAACTCA CGGACACCTG CAAAACGCTT GGGAAAGTCT TTGACGTGCA TTACTTCAAT
    ATTTTCAGTA CCTCAACACT AAAGAAAATA GCAGAGACCT TGTCATCTGA TGTGGAAGTT
    TTACTGCAGA TTGATGGTGT CACAGAAGAT AAACTGGAAA AATACGGAGC AGAAATAATT
    AAAGTGATGG ATAAGTACTC GGAGTGGAGC GTACCAGAAG ACACTGTTGG CCAAAGCGTG
    GACACGGCCG CGGGAAGCAC GGGCACACCC GAGAGCGAGG GAGAAGCCGA AGACGTCGTT
    ACCACCTCAA GCTATTTCTG CAATAACGCA AACCAAAGGA GGAAAAGGAA AAGGCCACCA
    AACTTCAGAG ACTCGAAGAG GAAAAAGACA AGCGCTGGCG GTAGCCAGCA GTTCCATCCC
    AAAGGGGCCC ACAGCAGGTA CCGGCGGCCC CGACGCCAGC CCGGGCCCAG GGCTCCCGGC
    CCCTCGCTGC CCGTCACCGC CTCCCAGGGA GCCGCCGCCA AGCTGGGGAT TATGGCACCT
    CCCAAACCCA AAAACAGGCA GTTCCTTCAG CCTTCGTATT CAGTACTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014808262.1
    CDS58..4308
    Translation

    Target ORF information:

    RefSeq Version XM_014952776.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax Bloom syndrome, RecQ helicase-like (BLM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014952776.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGGCCC TGCCGCAGAA CAACCTGCGG GAGCAGCTGC GGCTCCACTC GGCCCGCGGA 
    GCCCTCAGCG CCCTCCGCAA CGCGCCCCCG CCGCGCCAGC GCTCGGCGGG CTTCACCTTC
    AAGAAGACCT CTCCGGCCGG CGGCCCGCCC CGGGGCCTCC CCGCCGCCTC CGCCTTGAGG
    GAGAAGGATG TTAACACCTC CCTGGCTGCG CCGGCCTCCC CCCTCCCTGC CTCCAAGGAC
    AAGAAAACGC AAATCCAGGC GTTTTTTCCC GTGGTGTCCG GTGGGTGCAG CCTCCAGCCC
    AGCCCGGTGC TCGCCAGGCC TCAGGAGGCG TCAAGAGGCC CTGTGGCCTC TGGGCAGAGG
    AGCGTGAGTG AGGGGCTGCG AGGGACCCCC GCGCCCCGTC CCGTGGCTGT CCCCGCCGTC
    GGGGATGAGT GGGACGACAT TGATGACTTT GATTTGTCGG GAATCGAGAA GAAGTACTGC
    AGGCCGCCCG TCCTGTCCCC CAAGGGGCAG CGGGCCACCT GCAAGGCTTC CCAGAGGCCC
    GAGCCCCGCC GGGATGAGCT GCCCGGCTCT TCCCCCGGTG CTGTGGGCCA GGACACGGGG
    CCTGGCAGCC GTGGCACCCC GGGGCGTGGG GAGACCCCTC CAGAGGCCGA GCAACAACCC
    TCCTCGCAGC AGTCTGTGAT CTGCCTTGAG GATTCGGCTC CCTGCGGCGA TGAGAAGGCC
    GTGGGGTCTG GCGCTGGCGG CGGGGCCGGG CAGCGGGCGG GTGGCCAGGG GCAGAGCAGC
    CCCCTGGAAC TGGACGCCGT CGACATCGAG CCCTTCCCTG AGCTCGAGGA GGACGATTAC
    TTGGATGTCG TTCCACCATC CCCTGAGGAA GAGCTGCCTT CCTTCTTGCC TTCTGGAAAA
    AGCACTAGTA GTGTTTTCAA AGAGTCTCCT GCCAGTGGGA GGTCCACAAG CAGCTCTCAG
    TCCACGCCTG GGCCGGGTGC CACGGCGCAG CCTGTTGCTG ACAGTGACCC TGGTGCAGAG
    GGTGCAGATA AGGACCTGCC TTTGGAGCAG CAGCTCTTCG GCGTCATGGC CACCATCTGT
    GAGCTGGTGG ACGCCATCCC GCTCCGGGAG CTGCAGGGCC TCTCCTGCGC CAAGGCCCTG
    CTCCAGCAGC GAGACCTCAG GAGCAGATTG CTGGCTGGTT CTGTCACTTT GAACAAAAGC
    GCCATGACCA AGGGTTTTCC TGGTTCCTGG AAGGGGTGTG TGGAGCAGAG CTCTCCTGCC
    CCTCCTGCTC CGGGCTCTGG CCCAACCTGG AGCTTCAGTT TGGGCAGGAA CTCGCCCCAG
    TCCCCAAACC TCCCCTCTGC CCTTTCCGGG ACTGTTCCTT CAAGCCATTT TTCTACCAAG
    AAAAATCAAA CCTTGGACAC CCCTTGTGCT TCAAAGCCAA GTACCGAGGA GGTTACCTGC
    CCAGGAACTG CAGCACCATC CTCCTCCCGG GGACACGGCG GGGACAGAGC TTCCCTCAGC
    CCTCACCCTG AGACCTCCTT CCACAGCAGC TCCTGCGGGG AGCCGGGGCT GAGGAACGGG
    GAGAGCTGTC TCCTGCTGGA GAGGCCTCTC ACCAGCACCG CTCTGCGAGA CCAGGGCAGG
    GCTCCCACCA GCAGCTCTGA GCAGGACGCT CTGGGGCTGA ACCACACGGA CTTTGACCTC
    GATCACTTTG ATATCGACGA TTTTGATGAG GACTGGGAGA ATTCGGTGAA TGTTTCGGTA
    CCCGAGACGC CGTCGACGCC GCTGTATCAG CCCGTCAGGG AGGGGCCTCC TGCCAGATCG
    CTCTCCTCAA AGATCCTGTC CAGAGCCAAA GTCTCTGCTG CTGCATCAAA CCCTCCTGCT
    CCAAAATCCA GCTTCTTGAT GGCAACAAAG AACCACTCGG ACCCGCCGGC CACTAACCCT
    GCGCTGGACC GCTTCAGGGG CACGAAGTTC TCCCATTCTG AAGGGATGAT GAATATATTT
    CACAAGAAGT TTGGTTTGCA CTATTTTCGG ACAAACCAGC TGGAGGCCAT CAACGCTGCT
    CTGCTGGGTG AAGACTGTTT CATCCTGATG CCCACGGGGG GTGGGAAAAG CTTGTGCTAC
    CAGTTACCGG CCTGTGTCTC TGCTGGAGTC ACCATCGTCA TCTCCCCACT GAGGTCCCTG
    ATCATAGACC AAGTGCAGAA ACTGAAGACT TTAGATATTG CTGCAACATA TCTGACTGGC
    GATAGAACAG ATGCTGATGC TTCAAAAATA TACATGCAAC TGTCAAAAAA AGATCCTGTA
    ATAAAGCTTC TGTATGTTAC CCCTGAGAAG GTTTGTGCCA GCAACCGCCT GATGTCAGCC
    CTGGAAAATC TCTACGACAG GAAACTCCTG GAGCGTTTTG TCATCGACGA GGCCCACTGC
    GTCAGCCAGT GGGGTCACGA CTTCCGACAA GACTACAAGC GGCTGAACAT GCTGCGCAGG
    AGGTTCCGCT CCGTGCCCAT GATGGCTCTC ACGGCCACCG CCAACCCCAG GGTCCAGAAG
    GACATTCAGA ACCAGCTGGA GATGCTCAAA CCACAAGTGT TCACCATGAG CTTCAACAGG
    CACAACTTGA AATATGACGT GTTGCCCAAG AAGCCAAAGA AGGTGGCAAT GGATTGCTTG
    GAATGGATTA AGAAGTATCA CCCACATGAC TCTGGAATCA TTTATTGCCT TTCCCGACAT
    GAATGTGACA CGACAGCAGC TATCCTGCAG AAGGAAGGCC TTGCTGCACT GGCCTATCAC
    GCTGGTCTCA CCGACTCCAA CAGGGATCTG GTACAGAAGA AGTGGATTAA CCAAGAAGGG
    TGTCAGGTGA TTTGTGCCAC GATTGCCTTT GGAATGGGAA TCGACAAGCC CGACGTGCGC
    TACGTTATCC ACGCCTCCCT GCCCAAGTCT GTGGAAGGGT ACTACCAGGA GTCGGGCAGA
    GCGGGGCGGG ACGGGGAGAT GTCTCACTGC CTGCTCTTCT ACAGCTACAG CGACGTCACC
    CGGCTCCGCA GGCTGATACT CATGGAGAAG GATGGGAACA GCCACACGAG GCAGACCCAC
    TTTAACAACC TGTACAGTAT GGTTCACTAC TGTGAGAATT TGGTCGACTG CCGGAGAATC
    CAGCTTTTGG CCTACTTTGG GGAGACTAAT TTCAATCCCA CCTTCTGTAA AGATCACCCA
    GAGGTTACTT GCGACAACTG CAGTAGAAAG AAGGATTACA AATCACGAGA CGTAACGGAT
    GACGTGAAGA GCATTGTGAG ATTTGTACAA GAGCACTGTG GACAAACGGG ACGAACAAAT
    GCAAAGAGAA ATGCAGGTTT TGGAAGATAC ACGCTGAATA TGATGGTAGA CATCTTCTTA
    GGTGCCAAGA GCGCGAAGGT TCAGTCTGGG ATATTTGGGA AGGGAGCTGC CTACTCGCGG
    CACAACGCCG AAAGGCTCTT CAGAAAACTG GTCTTGGACA AGATTCTAGA TGAAGACTTG
    TACATCACTG CTAACGACCA GGCTGTGGCA TATGTCATCC TGGGACAGAA GGCTCAGGCG
    GTGCTCAACG GGTCCCTGCA GGTGGAGTTT CATGAAACGG AAAGCGCCAG CGCCGTCAGA
    AAGCAAAGGG CTTCTGTGGC AAAGGTGTCC CAGCGGGAAG AGATGGTTAA AAAGTGCCTT
    GGGGAACTCA CGGACACCTG CAAAACGCTT GGGAAAGTCT TTGACGTGCA TTACTTCAAT
    ATTTTCAGTA CCTCAACACT AAAGAAAATA GCAGAGACCT TGTCATCTGA TGTGGAAGTT
    TTACTGCAGA TTGATGGTGT CACAGAAGAT AAACTGGAAA AATACGGAGC AGAAATAATT
    AAAGTGATGG ATAAGTACTC GGAGTGGAGC GTACCAGAAG ACACTGTTGG CCAAAGCGTG
    GACACGGCCG CGGGAAGCAC GGGCACACCC GAGAGCGAGG GAGAAGCCGA AGACGTCGTT
    ACCACCTCAA GCTATTTCTG CAATAACGCA AACCAAAGGA GGAAAAGGAA AAGGCCACCA
    AACTTCAGAG ACTCGAAGAG GAAAAAGACA AGCGCTGGCG GTAGCCAGCA GTTCCATCCC
    AAAGGGGCCC ACAGCAGGTA CCGGCGGCCC CGACGCCAGC CCGGGCCCAG GGCTCCCGGC
    CCCTCGCTGC CCGTCACCGC CTCCCAGGGA GCCGCCGCCA AGCTGGGGAT TATGGCACCT
    CCCAAACCCA AAAACAGGCA GTTCCTTCAG CCTTCGTATT CAGTACTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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