MROH1 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	MROH1
Entrez Gene ID	106896313
Full Name	maestro heat like repeat family member 1
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014956146.1	XP_014811632.1	maestro heat-like repeat-containing protein family member 1 isoform X1
XM_014956148.1	XP_014811634.1	maestro heat-like repeat-containing protein family member 1 isoform X3
XM_014956147.1	XP_014811633.1	maestro heat-like repeat-containing protein family member 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following MROH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MROH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa75385	XM_014956146.1 Latest version!	Calidris pugnax maestro heat-like repeat family member 1 (MROH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa75386	XM_014956147.1 Latest version!	Calidris pugnax maestro heat-like repeat family member 1 (MROH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa75387	XM_014956148.1 Latest version!	Calidris pugnax maestro heat-like repeat family member 1 (MROH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MROH1 ( XM_014956146.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811632.1 Calidris pugnax maestro heat-like repeat family member 1 (MROH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa75385
Clone ID Related Accession (Same CDS sequence)	XM_014956146.1
Accession Version	XM_014956146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	maestro heat-like repeat-containing protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091040.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGT CACGGATAAA ACGGCTTGCC ACGACGTTGA TGGATGCCAC CACGGATAAG 
GACCCGCTGG TCCAGGAGCA GATCTACAAT GCCCTGTGCT ACCTGGGGGA ATCCGAGCCG 
GAGGAGATCC TCAACTCCTG CGACGAGTAC CTCCGGCAGC ACGACAAGCT GGCCTACCCC 
CATCGGGTGA TCATCCTGAA GGCCATGGAG ACCGTGGTGA AGAACAACAT TGCCCTCTTG 
GACAAGAGCA CGGCGAAGGT CGTTATCTTC CTGGCGTCTA ATGAAATGAC CAAGTCAAAG 
GAGGTGATCC GGGACTGGCA ACAAGCCGCC AGCAGCGTCC TCGTGGCAGT GGGGCAGCGC 
TTCATCAACA AGGTGATGGA GGAGGTGCTC ACCAAGTTTC AGCCAGGGAT CCTGCCCCAC 
TACTTCGTCC TGCAGACGTT CGCCAACCTC TCGGTGTCCA ACGTGTTCGG GATGGTGCCT 
TTCCTCAACT CCATCCTGGG GACCATGCTG CCCATGCTGG GAATGGCCAA GCAGGACCAC 
ATGAAGGCTG TCTTCTGCTA CGCCCTGCAG CACTTCAGCG AGAGCATCCA GGAGTACCTG 
GCCAACTTGG ACAAGGCCCC GGACCCCACC GTCAGGAAGG ACACCTTCTC CAATGAGATC 
TTCAGCGCCT ACGAAGTGCT CTTCAACAGC TGGCTGCAGC ACCGGGAAGC CAAGCTGAGA 
CTGGCCGTGG TGGAGGCTCT GGGACCGATG AGTTACCTGA TGCCCAGCGA AAAGCTGGAG 
GAGCAGCTCC CGAAGCTGAT TCCAGGCATC CTCGCCCTCT ACAAGAAGCA CACGGAGGCC 
TTCTACATAT CCAAGAGCCT CTGCCAGATC CTGGAAGCTT CCGTGAACAT CGGCAGCCGC 
AGCCTGGACG TGCAGCTGGA CTCTCTGCTG GGCACCCTGC ACCCCCAGAT TTGTGCCCCT 
GCGGATCCGT CTGTCCCCCT GACTGTTAAA AATCACACCG AGGTTCTGCG ATGCTTCACC 
GTCCTGGCCT GCTCCTTCCC TGACCGCGTG CTGGCCTTCC TCCTCCCCAA GCTGGAGAGC 
AGCAACGAGA GGACACGGGT GGGGACGCTC CTCATCATGA GGCAGATCAT CAACAGCGCC 
CCTTCTCAAA TGGAGATTAA AAAGCCTTTT ATTCTCTCAT CTATGAAACT CCCTCTGCAA 
GACAGCAATA ATAAGGTGAA GCGGGCGGTG GTGCAGGTGA TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCCGG CGGCGAGGCC ATGATGGAGT TCCTGGTCCG ACAGTGCGCC 
CTGCCCTCCG ACGCGCAGCC CAAAAAGCAG CCGCTGGATG CCGATGACCT CACTAGTGAC 
AGCGTCCAGA GCATCAGTGT CAATACGCTC TTCCTCCTCA GCACGACCGT TGACAGGATG 
AACAATGTTC TCTGGCCGTA CCTCCTGGAG TTTGTGACTC CCATACAGTT CACCAACGCC 
CTCACCCCGC TCTGCAAGAG CCTCATGTAC TTGGCCACGA AGAAGCAAGA GGAGGGAGAA 
AACACCTCCC TCATCCGTTA CGACCTGAAC GCAAACCTTC CTTCGCCCTA CGCTCTGACG 
ACGAGGCTGC TGGTCGTGTC TTCCCAGCCC TACGCAGGGG ACTGCCGGGG GGTGGCTGCC 
CTGCGGCTGC TCAGCGTCTT GCATTACAGC GTCCATCCTG CCCTGGACCA GCTCTGGAGC 
AAGCAGGTCC CTCTCTTGGT GGAGCACATA GAAGAGAACA CGGAGAAGTC CCTGTCCCGG 
AAGGAGTGGG AAGAGAAGCT GCTGCTGTTC CTCCAGGAGA CGCTGGCGGC CGTTGCTGAC 
AACGCCTGGA TTTGTCACTT TGTCACCGAG ATGTGCCGGC AGCTGAGCAG CTACAACGGC 
CTCCCGCTGG AGAAGAATTT CCTGTATAAA TGCATTGGGA CGACGCTCGG GGCCTGCACC 
AGCAAAGACC TTGTGCAGAA GCAGCTCCAG GAGCTCTTGG AAACAGCCAG ATACCAAGAG 
GAGGCAGAAA GGGAGGGGCT TGCTTCCTGC TTTGGGATAT GTGCAATAAA TCACCTGGAG 
GAAACCCTGG CCAAGCTGGA AGATTTCGTT AGGTCTGATG TCTTCAAGAA ATCCGTGGGG 
CTGTTCAGTC TCTTCAAGGA CCGTAGTGAT AATGAAGTGG AGAAAATCAA GAGCAGCCTC 
ATCCTCTGCT ACGGCTACGT CGCTGTCTAC GCGCCCAAGG AGCTGGTGCT GGCCAGGATC 
GAAGCAGACA TCCTGAAGAA CATCTTCCAG TACTTCAACA CTAAGGTTCT GGGAATAAAG 
GTAGAAACCA AGGACCTGAC CCTGAAGCTG TGCCTCATCC GGAGCATCTG CATGATCAGC 
CAAGCCATCT GCAACGGCGT CAAGTGCGGA GACTTCATCT TCTCCCGCAA AGCCGAGCTC 
GTGGCCCAGA TGGTGGAGTT CATCAAAGCC GAGCCGCTGG AGGCCCTGCG GACGCCGGTG 
CGGCAGCGAG CCATGGTCGC CTGCACTTAC CTGGTCATCC TGGAGCCCCA CCTCAGCGAC 
CCCGACAGGA CGGAGCTGAT GGACACCTGC CTCGCCAGCG TGCTGGCCCT CCCGCCGCCG 
GAGCTGGCCA AGGAGAGGGA GGAGAACGGA GGGGATGCCC TGCACAAGGA GATGCTGTAC 
GGTGACACCA TGGGTGCCCT TAAAGACCTG CTGAAGAGCC TCCTGCAGAG GAACTTGACC 
CCCCACGGGC TGCAGGACAT GTTTGCGCAT TTAGGCCCCT GGATCAAGTC GAGCAAGGAG 
CACGAGCGGC AGCGGGCGGT GGAGGTCAGC GCCGCCTTGC TGGACTTCTA CCTCAGCAAG 
CTGAATGTCA GTACCGTCAT CCCCTTCTAC AACCTCGGCG TCCTCATTGC CCTCTTCTCC 
CCGCGCTGCT CGGACTCCCT GGCCAGCGTC CGCCAACACG CCGTCGACTG CGTCTACTGC 
CTGCTCTACA TCCAGCTCTG CTACGAAGGG TTCGCCCGGG ATCACCGAGA TGAGATGGTG 
GAGGGGCTGA AAGCTCTGAA GAAAGGCCTG GAGGAGCCCG ATTTCACCGT TCTCTTCCAC 
ACCTGCAACA ACATCGCCAT GGTGATCGGG AAGCGTGTCC CTCCGGACCA GCTGATGAGC 
CTGCTGCTGG CCATGTTCGA GGGGCTGGCG GACCCCGATA AGAACTGCTC CCGAGCAGCC 
ACCGTCATGA TCAACTCCCT CCTCAAGGAG CGCGGAGCCG TCTTGCAGGA GAAGGTGCCC 
GACATCATGA GCATCATCCA CAGCAAGCTG GAGGAGGTGG ACGAGGAGCA CATCCGGAAG 
GCGGCCCAGC AGACGGTCTA CATCCTGGCC GCCCAGCACA AGAGCGTGGT GGTCTCCAGC 
CTGCTGGGCA GCCCCCTGCC CTTCGACAGC CACACCTGCG CCATGTGGAG GTCCCTGGCC 
ACCGAGCCCA CCCTCACCTC GCAGATCCTG GAGCAGCTCC TGGAGAAGGT CAACAGGGAC 
GTCCCCTACA AGGAGAGCAA GTCCTTCCCT CTGCTGGGGA GCGGATCCGA GCGCGTGGCC 
ACCCCCCTGC CCCTGGCCGC CACATGTGCT CTGTATGAGA TCATGTCTGC TGCCGAGTCT 
GGCCCGGCCG TGCTGGGGCT CTACCCGCCC TTGTTTGTCA CCCTCCTGCT GCGCCTCAGC 
TGCACCGTGG GTGTCCAGCT CCCCAGGAAC CTGCAGAGCC GCGAGAGGAG GAGCAGCTCC 
AGCCTCGGCC CCGCTCCCCG CAGCCTCCAG CCCTGCAGCT GCGCCGTGGA GACGCTGAAG 
GTGATGCTGG TCCGGGGGGG CAGTGAGGAG GTGGCCAGGG CGGTGGGCAG CGCCGGCGGC 
TGGGAGCTGA TGGCCAGTCC AGAGAGGCAC CACGATGGCA TCGCCGTGCT GGCCAGGGCC 
ATGGCACGCC ACGCCGGCCC CCGGCTCCCC CTCATCGTGA AGAACCTCAT CCCCACCCTC 
AACAGCGTCT TCGACAGCCA GAGGGTCACC ACCACCGCCT TCTTCGCCGA GCTCCTCAAC 
AGTAACGTGG TGAATGACCT GATCCTGCTG GAGACCATGA TGGACAACAT GACGGGGAGG 
CAGAAGGACT CCTGCATGTT GGTGCGGATG CTGGCGCTGC GGGGACTCGG CAACATCGCC 
TCTGGCTCGC CCGAAAAGGT GAGGAAGCAC GGGGCCAAGC TCCTGGCCTC CATGGTCAAC 
GGCATGGATG ACAAAGATGA CCCCCAGAAC CTGGTGGCCC TGGAGGCCAT GTCCAGCCTC 
TCCAAGCTCC TGGATCACCT GGAGGAGAGG GACGTCCAGT CCATGCTCCT CCACATCGCC 
ATCAGGATCC GGCCCTTCTT CGACAGCGAG CAGCCCGACC TCCGCCAGTC CTCCATCGTC 
CTCTTCGGCA ACCTCACCAA GTTCAGCCAA GGCCACTGCG AGGTCTTCGT TGAGCAGATC 
CTCAACGGGC TGGTGACGCT GCTGCTGCAC CTCCAGGACC CAAAGCCAGA GGTGGTCAAA 
GCCTGCAAGT TCGCCCTGCG CATGTGTGGC CCCAACATGG GCTGCGAGGG GCTCTGCGAC 
ATGTTCCTCA ACCACCTGCA GGAGGAGAGG AGCCTGCACT ACGGGGAGTT CATGAACAAC 
GTCTGCAAAC ACTTGATGCA GTGCTACCCG GAGATGCTGA ACCGCCTCAT CTTAACCAAC 
CTCTTCTACT TCAAGAGCAA CTGGGTGGAC ATCCGAGCTG CTGCCCCCAT GTTCATCGGC 
TTCCTCGTGC TGCACGTGGA TGAAGAGCAG TGCCAGCAAG TGGACCTGGA CCAGCTCATT 
TCGGCTCTGA ACCTGCTCCT GAAGGACCCC GTCCCCGCCG TCCGCATGAA GGTGGCGGAG 
ACGTTGGGCC GCCTCGTCCG GATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014811632.1
CDS	357..5306
Translation	MTESRIKRLATTLMDATTDKDPLVQEQIYNALCYLGESEPEEILNSCDEYLRQHDKLAYPHRVIILKAMETVVKNNIALLDKSTAKVVIFLASNEMTKSKEVIRDWQQAASSVLVAVGQRFINKVMEEVLTKFQPGILPHYFVLQTFANLSVSNVFGMVPFLNSILGTMLPMLGMAKQDHMKAVFCYALQHFSESIQEYLANLDKAPDPTVRKDTFSNEIFSAYEVLFNSWLQHREAKLRLAVVEALGPMSYLMPSEKLEEQLPKLIPGILALYKKHTEAFYISKSLCQILEASVNIGSRSLDVQLDSLLGTLHPQICAPADPSVPLTVKNHTEVLRCFTVLACSFPDRVLAFLLPKLESSNERTRVGTLLIMRQIINSAPSQMEIKKPFILSSMKLPLQDSNNKVKRAVVQVISAMAHHGYLEQPGGEAMMEFLVRQCALPSDAQPKKQPLDADDLTSDSVQSISVNTLFLLSTTVDRMNNVLWPYLLEFVTPIQFTNALTPLCKSLMYLATKKQEEGENTSLIRYDLNANLPSPYALTTRLLVVSSQPYAGDCRGVAALRLLSVLHYSVHPALDQLWSKQVPLLVEHIEENTEKSLSRKEWEEKLLLFLQETLAAVADNAWICHFVTEMCRQLSSYNGLPLEKNFLYKCIGTTLGACTSKDLVQKQLQELLETARYQEEAEREGLASCFGICAINHLEETLAKLEDFVRSDVFKKSVGLFSLFKDRSDNEVEKIKSSLILCYGYVAVYAPKELVLARIEADILKNIFQYFNTKVLGIKVETKDLTLKLCLIRSICMISQAICNGVKCGDFIFSRKAELVAQMVEFIKAEPLEALRTPVRQRAMVACTYLVILEPHLSDPDRTELMDTCLASVLALPPPELAKEREENGGDALHKEMLYGDTMGALKDLLKSLLQRNLTPHGLQDMFAHLGPWIKSSKEHERQRAVEVSAALLDFYLSKLNVSTVIPFYNLGVLIALFSPRCSDSLASVRQHAVDCVYCLLYIQLCYEGFARDHRDEMVEGLKALKKGLEEPDFTVLFHTCNNIAMVIGKRVPPDQLMSLLLAMFEGLADPDKNCSRAATVMINSLLKERGAVLQEKVPDIMSIIHSKLEEVDEEHIRKAAQQTVYILAAQHKSVVVSSLLGSPLPFDSHTCAMWRSLATEPTLTSQILEQLLEKVNRDVPYKESKSFPLLGSGSERVATPLPLAATCALYEIMSAAESGPAVLGLYPPLFVTLLLRLSCTVGVQLPRNLQSRERRSSSSLGPAPRSLQPCSCAVETLKVMLVRGGSEEVARAVGSAGGWELMASPERHHDGIAVLARAMARHAGPRLPLIVKNLIPTLNSVFDSQRVTTTAFFAELLNSNVVNDLILLETMMDNMTGRQKDSCMLVRMLALRGLGNIASGSPEKVRKHGAKLLASMVNGMDDKDDPQNLVALEAMSSLSKLLDHLEERDVQSMLLHIAIRIRPFFDSEQPDLRQSSIVLFGNLTKFSQGHCEVFVEQILNGLVTLLLHLQDPKPEVVKACKFALRMCGPNMGCEGLCDMFLNHLQEERSLHYGEFMNNVCKHLMQCYPEMLNRLILTNLFYFKSNWVDIRAAAPMFIGFLVLHVDEEQCQQVDLDQLISALNLLLKDPVPAVRMKVAETLGRLVRIL

Target ORF information:

RefSeq Version	XM_014956146.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax maestro heat-like repeat family member 1 (MROH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956146.1

ORF Insert Sequence:

ATGACGGAGT CACGGATAAA ACGGCTTGCC ACGACGTTGA TGGATGCCAC CACGGATAAG 
GACCCGCTGG TCCAGGAGCA GATCTACAAT GCCCTGTGCT ACCTGGGGGA ATCCGAGCCG 
GAGGAGATCC TCAACTCCTG CGACGAGTAC CTCCGGCAGC ACGACAAGCT GGCCTACCCC 
CATCGGGTGA TCATCCTGAA GGCCATGGAG ACCGTGGTGA AGAACAACAT TGCCCTCTTG 
GACAAGAGCA CGGCGAAGGT CGTTATCTTC CTGGCGTCTA ATGAAATGAC CAAGTCAAAG 
GAGGTGATCC GGGACTGGCA ACAAGCCGCC AGCAGCGTCC TCGTGGCAGT GGGGCAGCGC 
TTCATCAACA AGGTGATGGA GGAGGTGCTC ACCAAGTTTC AGCCAGGGAT CCTGCCCCAC 
TACTTCGTCC TGCAGACGTT CGCCAACCTC TCGGTGTCCA ACGTGTTCGG GATGGTGCCT 
TTCCTCAACT CCATCCTGGG GACCATGCTG CCCATGCTGG GAATGGCCAA GCAGGACCAC 
ATGAAGGCTG TCTTCTGCTA CGCCCTGCAG CACTTCAGCG AGAGCATCCA GGAGTACCTG 
GCCAACTTGG ACAAGGCCCC GGACCCCACC GTCAGGAAGG ACACCTTCTC CAATGAGATC 
TTCAGCGCCT ACGAAGTGCT CTTCAACAGC TGGCTGCAGC ACCGGGAAGC CAAGCTGAGA 
CTGGCCGTGG TGGAGGCTCT GGGACCGATG AGTTACCTGA TGCCCAGCGA AAAGCTGGAG 
GAGCAGCTCC CGAAGCTGAT TCCAGGCATC CTCGCCCTCT ACAAGAAGCA CACGGAGGCC 
TTCTACATAT CCAAGAGCCT CTGCCAGATC CTGGAAGCTT CCGTGAACAT CGGCAGCCGC 
AGCCTGGACG TGCAGCTGGA CTCTCTGCTG GGCACCCTGC ACCCCCAGAT TTGTGCCCCT 
GCGGATCCGT CTGTCCCCCT GACTGTTAAA AATCACACCG AGGTTCTGCG ATGCTTCACC 
GTCCTGGCCT GCTCCTTCCC TGACCGCGTG CTGGCCTTCC TCCTCCCCAA GCTGGAGAGC 
AGCAACGAGA GGACACGGGT GGGGACGCTC CTCATCATGA GGCAGATCAT CAACAGCGCC 
CCTTCTCAAA TGGAGATTAA AAAGCCTTTT ATTCTCTCAT CTATGAAACT CCCTCTGCAA 
GACAGCAATA ATAAGGTGAA GCGGGCGGTG GTGCAGGTGA TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCCGG CGGCGAGGCC ATGATGGAGT TCCTGGTCCG ACAGTGCGCC 
CTGCCCTCCG ACGCGCAGCC CAAAAAGCAG CCGCTGGATG CCGATGACCT CACTAGTGAC 
AGCGTCCAGA GCATCAGTGT CAATACGCTC TTCCTCCTCA GCACGACCGT TGACAGGATG 
AACAATGTTC TCTGGCCGTA CCTCCTGGAG TTTGTGACTC CCATACAGTT CACCAACGCC 
CTCACCCCGC TCTGCAAGAG CCTCATGTAC TTGGCCACGA AGAAGCAAGA GGAGGGAGAA 
AACACCTCCC TCATCCGTTA CGACCTGAAC GCAAACCTTC CTTCGCCCTA CGCTCTGACG 
ACGAGGCTGC TGGTCGTGTC TTCCCAGCCC TACGCAGGGG ACTGCCGGGG GGTGGCTGCC 
CTGCGGCTGC TCAGCGTCTT GCATTACAGC GTCCATCCTG CCCTGGACCA GCTCTGGAGC 
AAGCAGGTCC CTCTCTTGGT GGAGCACATA GAAGAGAACA CGGAGAAGTC CCTGTCCCGG 
AAGGAGTGGG AAGAGAAGCT GCTGCTGTTC CTCCAGGAGA CGCTGGCGGC CGTTGCTGAC 
AACGCCTGGA TTTGTCACTT TGTCACCGAG ATGTGCCGGC AGCTGAGCAG CTACAACGGC 
CTCCCGCTGG AGAAGAATTT CCTGTATAAA TGCATTGGGA CGACGCTCGG GGCCTGCACC 
AGCAAAGACC TTGTGCAGAA GCAGCTCCAG GAGCTCTTGG AAACAGCCAG ATACCAAGAG 
GAGGCAGAAA GGGAGGGGCT TGCTTCCTGC TTTGGGATAT GTGCAATAAA TCACCTGGAG 
GAAACCCTGG CCAAGCTGGA AGATTTCGTT AGGTCTGATG TCTTCAAGAA ATCCGTGGGG 
CTGTTCAGTC TCTTCAAGGA CCGTAGTGAT AATGAAGTGG AGAAAATCAA GAGCAGCCTC 
ATCCTCTGCT ACGGCTACGT CGCTGTCTAC GCGCCCAAGG AGCTGGTGCT GGCCAGGATC 
GAAGCAGACA TCCTGAAGAA CATCTTCCAG TACTTCAACA CTAAGGTTCT GGGAATAAAG 
GTAGAAACCA AGGACCTGAC CCTGAAGCTG TGCCTCATCC GGAGCATCTG CATGATCAGC 
CAAGCCATCT GCAACGGCGT CAAGTGCGGA GACTTCATCT TCTCCCGCAA AGCCGAGCTC 
GTGGCCCAGA TGGTGGAGTT CATCAAAGCC GAGCCGCTGG AGGCCCTGCG GACGCCGGTG 
CGGCAGCGAG CCATGGTCGC CTGCACTTAC CTGGTCATCC TGGAGCCCCA CCTCAGCGAC 
CCCGACAGGA CGGAGCTGAT GGACACCTGC CTCGCCAGCG TGCTGGCCCT CCCGCCGCCG 
GAGCTGGCCA AGGAGAGGGA GGAGAACGGA GGGGATGCCC TGCACAAGGA GATGCTGTAC 
GGTGACACCA TGGGTGCCCT TAAAGACCTG CTGAAGAGCC TCCTGCAGAG GAACTTGACC 
CCCCACGGGC TGCAGGACAT GTTTGCGCAT TTAGGCCCCT GGATCAAGTC GAGCAAGGAG 
CACGAGCGGC AGCGGGCGGT GGAGGTCAGC GCCGCCTTGC TGGACTTCTA CCTCAGCAAG 
CTGAATGTCA GTACCGTCAT CCCCTTCTAC AACCTCGGCG TCCTCATTGC CCTCTTCTCC 
CCGCGCTGCT CGGACTCCCT GGCCAGCGTC CGCCAACACG CCGTCGACTG CGTCTACTGC 
CTGCTCTACA TCCAGCTCTG CTACGAAGGG TTCGCCCGGG ATCACCGAGA TGAGATGGTG 
GAGGGGCTGA AAGCTCTGAA GAAAGGCCTG GAGGAGCCCG ATTTCACCGT TCTCTTCCAC 
ACCTGCAACA ACATCGCCAT GGTGATCGGG AAGCGTGTCC CTCCGGACCA GCTGATGAGC 
CTGCTGCTGG CCATGTTCGA GGGGCTGGCG GACCCCGATA AGAACTGCTC CCGAGCAGCC 
ACCGTCATGA TCAACTCCCT CCTCAAGGAG CGCGGAGCCG TCTTGCAGGA GAAGGTGCCC 
GACATCATGA GCATCATCCA CAGCAAGCTG GAGGAGGTGG ACGAGGAGCA CATCCGGAAG 
GCGGCCCAGC AGACGGTCTA CATCCTGGCC GCCCAGCACA AGAGCGTGGT GGTCTCCAGC 
CTGCTGGGCA GCCCCCTGCC CTTCGACAGC CACACCTGCG CCATGTGGAG GTCCCTGGCC 
ACCGAGCCCA CCCTCACCTC GCAGATCCTG GAGCAGCTCC TGGAGAAGGT CAACAGGGAC 
GTCCCCTACA AGGAGAGCAA GTCCTTCCCT CTGCTGGGGA GCGGATCCGA GCGCGTGGCC 
ACCCCCCTGC CCCTGGCCGC CACATGTGCT CTGTATGAGA TCATGTCTGC TGCCGAGTCT 
GGCCCGGCCG TGCTGGGGCT CTACCCGCCC TTGTTTGTCA CCCTCCTGCT GCGCCTCAGC 
TGCACCGTGG GTGTCCAGCT CCCCAGGAAC CTGCAGAGCC GCGAGAGGAG GAGCAGCTCC 
AGCCTCGGCC CCGCTCCCCG CAGCCTCCAG CCCTGCAGCT GCGCCGTGGA GACGCTGAAG 
GTGATGCTGG TCCGGGGGGG CAGTGAGGAG GTGGCCAGGG CGGTGGGCAG CGCCGGCGGC 
TGGGAGCTGA TGGCCAGTCC AGAGAGGCAC CACGATGGCA TCGCCGTGCT GGCCAGGGCC 
ATGGCACGCC ACGCCGGCCC CCGGCTCCCC CTCATCGTGA AGAACCTCAT CCCCACCCTC 
AACAGCGTCT TCGACAGCCA GAGGGTCACC ACCACCGCCT TCTTCGCCGA GCTCCTCAAC 
AGTAACGTGG TGAATGACCT GATCCTGCTG GAGACCATGA TGGACAACAT GACGGGGAGG 
CAGAAGGACT CCTGCATGTT GGTGCGGATG CTGGCGCTGC GGGGACTCGG CAACATCGCC 
TCTGGCTCGC CCGAAAAGGT GAGGAAGCAC GGGGCCAAGC TCCTGGCCTC CATGGTCAAC 
GGCATGGATG ACAAAGATGA CCCCCAGAAC CTGGTGGCCC TGGAGGCCAT GTCCAGCCTC 
TCCAAGCTCC TGGATCACCT GGAGGAGAGG GACGTCCAGT CCATGCTCCT CCACATCGCC 
ATCAGGATCC GGCCCTTCTT CGACAGCGAG CAGCCCGACC TCCGCCAGTC CTCCATCGTC 
CTCTTCGGCA ACCTCACCAA GTTCAGCCAA GGCCACTGCG AGGTCTTCGT TGAGCAGATC 
CTCAACGGGC TGGTGACGCT GCTGCTGCAC CTCCAGGACC CAAAGCCAGA GGTGGTCAAA 
GCCTGCAAGT TCGCCCTGCG CATGTGTGGC CCCAACATGG GCTGCGAGGG GCTCTGCGAC 
ATGTTCCTCA ACCACCTGCA GGAGGAGAGG AGCCTGCACT ACGGGGAGTT CATGAACAAC 
GTCTGCAAAC ACTTGATGCA GTGCTACCCG GAGATGCTGA ACCGCCTCAT CTTAACCAAC 
CTCTTCTACT TCAAGAGCAA CTGGGTGGAC ATCCGAGCTG CTGCCCCCAT GTTCATCGGC 
TTCCTCGTGC TGCACGTGGA TGAAGAGCAG TGCCAGCAAG TGGACCTGGA CCAGCTCATT 
TCGGCTCTGA ACCTGCTCCT GAAGGACCCC GTCCCCGCCG TCCGCATGAA GGTGGCGGAG 
ACGTTGGGCC GCCTCGTCCG GATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_014956147.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811633.1 Calidris pugnax maestro heat-like repeat family member 1 (MROH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa75386
Clone ID Related Accession (Same CDS sequence)	XM_014956147.1
Accession Version	XM_014956147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	maestro heat-like repeat-containing protein family member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091040.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGT CACGGATAAA ACGGCTTGCC ACGACGTTGA TGGATGCCAC CACGGATAAG 
GACCCGCTGG TCCAGGAGCA GATCTACAAT GCCCTGTGCT ACCTGGGGGA ATCCGAGCCG 
GAGGAGATCC TCAACTCCTG CGACGAGTAC CTCCGGCAGC ACGACAAGCT GGCCTACCCC 
CATCGGGTGA TCATCCTGAA GGCCATGGAG ACCGTGGTGA AGAACAACAT TGCCCTCTTG 
GACAAGAGCA CGGCGAAGGT CGTTATCTTC CTGGCGTCTA ATGAAATGAC CAAGTCAAAG 
GAGGTGATCC GGGACTGGCA ACAAGCCGCC AGCAGCGTCC TCGTGGCAGT GGGGCAGCGC 
TTCATCAACA AGGTGATGGA GGAGGTGCTC ACCAAGTTTC AGCCAGGGAT CCTGCCCCAC 
TACTTCGTCC TGCAGACGTT CGCCAACCTC TCGGTGTCCA ACGTGTTCGG GATGGTGCCT 
TTCCTCAACT CCATCCTGGG GACCATGCTG CCCATGCTGG GAATGGCCAA GCAGGACCAC 
ATGAAGGCTG TCTTCTGCTA CGCCCTGCAG CACTTCAGCG AGAGCATCCA GGAGTACCTG 
GCCAACTTGG ACAAGGCCCC GGACCCCACC GTCAGGAAGG ACACCTTCTC CAATGAGATC 
TTCAGCGCCT ACGAAGTGCT CTTCAACAGC TGGCTGCAGC ACCGGGAAGC CAAGCTGAGA 
CTGGCCGTGG TGGAGGCTCT GGGACCGATG AGTTACCTGA TGCCCAGCGA AAAGCTGGAG 
GAGCAGCTCC CGAAGCTGAT TCCAGGCATC CTCGCCCTCT ACAAGAAGCA CACGGAGGCC 
TTCTACATAT CCAAGAGCCT CTGCCAGATC CTGGAAGCTT CCGTGAACAT CGGCAGCCGC 
AGCCTGGACG TGCAGCTGGA CTCTCTGCTG GGCACCCTGC ACCCCCAGAT TTGTGCCCCT 
GCGGATCCGT CTGTCCCCCT GACTGTTAAA AATCACACCG AGGTTCTGCG ATGCTTCACC 
GTCCTGGCCT GCTCCTTCCC TGACCGCGTG CTGGCCTTCC TCCTCCCCAA GCTGGAGAGC 
AGCAACGAGA GGACACGGGT GGGGACGCTC CTCATCATGA GGCAGATCAT CAACAGCGCC 
CCTTCTCAAA TGGAGATTAA AAAGCCTTTT ATTCTCTCAT CTATGAAACT CCCTCTGCAA 
GACAGCAATA ATAAGGTGAA GCGGGCGGTG GTGCAGGTGA TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCCGG CGGCGAGGCC ATGATGGAGT TCCTGGTCCG ACAGTGCGCC 
CTGCCCTCCG ACGCGCCCAA AAAGCAGCCG CTGGATGCCG ATGACCTCAC TAGTGACAGC 
GTCCAGAGCA TCAGTGTCAA TACGCTCTTC CTCCTCAGCA CGACCGTTGA CAGGATGAAC 
AATGTTCTCT GGCCGTACCT CCTGGAGTTT GTGACTCCCA TACAGTTCAC CAACGCCCTC 
ACCCCGCTCT GCAAGAGCCT CATGTACTTG GCCACGAAGA AGCAAGAGGA GGGAGAAAAC 
ACCTCCCTCA TCCGTTACGA CCTGAACGCA AACCTTCCTT CGCCCTACGC TCTGACGACG 
AGGCTGCTGG TCGTGTCTTC CCAGCCCTAC GCAGGGGACT GCCGGGGGGT GGCTGCCCTG 
CGGCTGCTCA GCGTCTTGCA TTACAGCGTC CATCCTGCCC TGGACCAGCT CTGGAGCAAG 
CAGGTCCCTC TCTTGGTGGA GCACATAGAA GAGAACACGG AGAAGTCCCT GTCCCGGAAG 
GAGTGGGAAG AGAAGCTGCT GCTGTTCCTC CAGGAGACGC TGGCGGCCGT TGCTGACAAC 
GCCTGGATTT GTCACTTTGT CACCGAGATG TGCCGGCAGC TGAGCAGCTA CAACGGCCTC 
CCGCTGGAGA AGAATTTCCT GTATAAATGC ATTGGGACGA CGCTCGGGGC CTGCACCAGC 
AAAGACCTTG TGCAGAAGCA GCTCCAGGAG CTCTTGGAAA CAGCCAGATA CCAAGAGGAG 
GCAGAAAGGG AGGGGCTTGC TTCCTGCTTT GGGATATGTG CAATAAATCA CCTGGAGGAA 
ACCCTGGCCA AGCTGGAAGA TTTCGTTAGG TCTGATGTCT TCAAGAAATC CGTGGGGCTG 
TTCAGTCTCT TCAAGGACCG TAGTGATAAT GAAGTGGAGA AAATCAAGAG CAGCCTCATC 
CTCTGCTACG GCTACGTCGC TGTCTACGCG CCCAAGGAGC TGGTGCTGGC CAGGATCGAA 
GCAGACATCC TGAAGAACAT CTTCCAGTAC TTCAACACTA AGGTTCTGGG AATAAAGGTA 
GAAACCAAGG ACCTGACCCT GAAGCTGTGC CTCATCCGGA GCATCTGCAT GATCAGCCAA 
GCCATCTGCA ACGGCGTCAA GTGCGGAGAC TTCATCTTCT CCCGCAAAGC CGAGCTCGTG 
GCCCAGATGG TGGAGTTCAT CAAAGCCGAG CCGCTGGAGG CCCTGCGGAC GCCGGTGCGG 
CAGCGAGCCA TGGTCGCCTG CACTTACCTG GTCATCCTGG AGCCCCACCT CAGCGACCCC 
GACAGGACGG AGCTGATGGA CACCTGCCTC GCCAGCGTGC TGGCCCTCCC GCCGCCGGAG 
CTGGCCAAGG AGAGGGAGGA GAACGGAGGG GATGCCCTGC ACAAGGAGAT GCTGTACGGT 
GACACCATGG GTGCCCTTAA AGACCTGCTG AAGAGCCTCC TGCAGAGGAA CTTGACCCCC 
CACGGGCTGC AGGACATGTT TGCGCATTTA GGCCCCTGGA TCAAGTCGAG CAAGGAGCAC 
GAGCGGCAGC GGGCGGTGGA GGTCAGCGCC GCCTTGCTGG ACTTCTACCT CAGCAAGCTG 
AATGTCAGTA CCGTCATCCC CTTCTACAAC CTCGGCGTCC TCATTGCCCT CTTCTCCCCG 
CGCTGCTCGG ACTCCCTGGC CAGCGTCCGC CAACACGCCG TCGACTGCGT CTACTGCCTG 
CTCTACATCC AGCTCTGCTA CGAAGGGTTC GCCCGGGATC ACCGAGATGA GATGGTGGAG 
GGGCTGAAAG CTCTGAAGAA AGGCCTGGAG GAGCCCGATT TCACCGTTCT CTTCCACACC 
TGCAACAACA TCGCCATGGT GATCGGGAAG CGTGTCCCTC CGGACCAGCT GATGAGCCTG 
CTGCTGGCCA TGTTCGAGGG GCTGGCGGAC CCCGATAAGA ACTGCTCCCG AGCAGCCACC 
GTCATGATCA ACTCCCTCCT CAAGGAGCGC GGAGCCGTCT TGCAGGAGAA GGTGCCCGAC 
ATCATGAGCA TCATCCACAG CAAGCTGGAG GAGGTGGACG AGGAGCACAT CCGGAAGGCG 
GCCCAGCAGA CGGTCTACAT CCTGGCCGCC CAGCACAAGA GCGTGGTGGT CTCCAGCCTG 
CTGGGCAGCC CCCTGCCCTT CGACAGCCAC ACCTGCGCCA TGTGGAGGTC CCTGGCCACC 
GAGCCCACCC TCACCTCGCA GATCCTGGAG CAGCTCCTGG AGAAGGTCAA CAGGGACGTC 
CCCTACAAGG AGAGCAAGTC CTTCCCTCTG CTGGGGAGCG GATCCGAGCG CGTGGCCACC 
CCCCTGCCCC TGGCCGCCAC ATGTGCTCTG TATGAGATCA TGTCTGCTGC CGAGTCTGGC 
CCGGCCGTGC TGGGGCTCTA CCCGCCCTTG TTTGTCACCC TCCTGCTGCG CCTCAGCTGC 
ACCGTGGGTG TCCAGCTCCC CAGGAACCTG CAGAGCCGCG AGAGGAGGAG CAGCTCCAGC 
CTCGGCCCCG CTCCCCGCAG CCTCCAGCCC TGCAGCTGCG CCGTGGAGAC GCTGAAGGTG 
ATGCTGGTCC GGGGGGGCAG TGAGGAGGTG GCCAGGGCGG TGGGCAGCGC CGGCGGCTGG 
GAGCTGATGG CCAGTCCAGA GAGGCACCAC GATGGCATCG CCGTGCTGGC CAGGGCCATG 
GCACGCCACG CCGGCCCCCG GCTCCCCCTC ATCGTGAAGA ACCTCATCCC CACCCTCAAC 
AGCGTCTTCG ACAGCCAGAG GGTCACCACC ACCGCCTTCT TCGCCGAGCT CCTCAACAGT 
AACGTGGTGA ATGACCTGAT CCTGCTGGAG ACCATGATGG ACAACATGAC GGGGAGGCAG 
AAGGACTCCT GCATGTTGGT GCGGATGCTG GCGCTGCGGG GACTCGGCAA CATCGCCTCT 
GGCTCGCCCG AAAAGGTGAG GAAGCACGGG GCCAAGCTCC TGGCCTCCAT GGTCAACGGC 
ATGGATGACA AAGATGACCC CCAGAACCTG GTGGCCCTGG AGGCCATGTC CAGCCTCTCC 
AAGCTCCTGG ATCACCTGGA GGAGAGGGAC GTCCAGTCCA TGCTCCTCCA CATCGCCATC 
AGGATCCGGC CCTTCTTCGA CAGCGAGCAG CCCGACCTCC GCCAGTCCTC CATCGTCCTC 
TTCGGCAACC TCACCAAGTT CAGCCAAGGC CACTGCGAGG TCTTCGTTGA GCAGATCCTC 
AACGGGCTGG TGACGCTGCT GCTGCACCTC CAGGACCCAA AGCCAGAGGT GGTCAAAGCC 
TGCAAGTTCG CCCTGCGCAT GTGTGGCCCC AACATGGGCT GCGAGGGGCT CTGCGACATG 
TTCCTCAACC ACCTGCAGGA GGAGAGGAGC CTGCACTACG GGGAGTTCAT GAACAACGTC 
TGCAAACACT TGATGCAGTG CTACCCGGAG ATGCTGAACC GCCTCATCTT AACCAACCTC 
TTCTACTTCA AGAGCAACTG GGTGGACATC CGAGCTGCTG CCCCCATGTT CATCGGCTTC 
CTCGTGCTGC ACGTGGATGA AGAGCAGTGC CAGCAAGTGG ACCTGGACCA GCTCATTTCG 
GCTCTGAACC TGCTCCTGAA GGACCCCGTC CCCGCCGTCC GCATGAAGGT GGCGGAGACG 
TTGGGCCGCC TCGTCCGGAT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014811633.1
CDS	357..5303
Translation	MTESRIKRLATTLMDATTDKDPLVQEQIYNALCYLGESEPEEILNSCDEYLRQHDKLAYPHRVIILKAMETVVKNNIALLDKSTAKVVIFLASNEMTKSKEVIRDWQQAASSVLVAVGQRFINKVMEEVLTKFQPGILPHYFVLQTFANLSVSNVFGMVPFLNSILGTMLPMLGMAKQDHMKAVFCYALQHFSESIQEYLANLDKAPDPTVRKDTFSNEIFSAYEVLFNSWLQHREAKLRLAVVEALGPMSYLMPSEKLEEQLPKLIPGILALYKKHTEAFYISKSLCQILEASVNIGSRSLDVQLDSLLGTLHPQICAPADPSVPLTVKNHTEVLRCFTVLACSFPDRVLAFLLPKLESSNERTRVGTLLIMRQIINSAPSQMEIKKPFILSSMKLPLQDSNNKVKRAVVQVISAMAHHGYLEQPGGEAMMEFLVRQCALPSDAPKKQPLDADDLTSDSVQSISVNTLFLLSTTVDRMNNVLWPYLLEFVTPIQFTNALTPLCKSLMYLATKKQEEGENTSLIRYDLNANLPSPYALTTRLLVVSSQPYAGDCRGVAALRLLSVLHYSVHPALDQLWSKQVPLLVEHIEENTEKSLSRKEWEEKLLLFLQETLAAVADNAWICHFVTEMCRQLSSYNGLPLEKNFLYKCIGTTLGACTSKDLVQKQLQELLETARYQEEAEREGLASCFGICAINHLEETLAKLEDFVRSDVFKKSVGLFSLFKDRSDNEVEKIKSSLILCYGYVAVYAPKELVLARIEADILKNIFQYFNTKVLGIKVETKDLTLKLCLIRSICMISQAICNGVKCGDFIFSRKAELVAQMVEFIKAEPLEALRTPVRQRAMVACTYLVILEPHLSDPDRTELMDTCLASVLALPPPELAKEREENGGDALHKEMLYGDTMGALKDLLKSLLQRNLTPHGLQDMFAHLGPWIKSSKEHERQRAVEVSAALLDFYLSKLNVSTVIPFYNLGVLIALFSPRCSDSLASVRQHAVDCVYCLLYIQLCYEGFARDHRDEMVEGLKALKKGLEEPDFTVLFHTCNNIAMVIGKRVPPDQLMSLLLAMFEGLADPDKNCSRAATVMINSLLKERGAVLQEKVPDIMSIIHSKLEEVDEEHIRKAAQQTVYILAAQHKSVVVSSLLGSPLPFDSHTCAMWRSLATEPTLTSQILEQLLEKVNRDVPYKESKSFPLLGSGSERVATPLPLAATCALYEIMSAAESGPAVLGLYPPLFVTLLLRLSCTVGVQLPRNLQSRERRSSSSLGPAPRSLQPCSCAVETLKVMLVRGGSEEVARAVGSAGGWELMASPERHHDGIAVLARAMARHAGPRLPLIVKNLIPTLNSVFDSQRVTTTAFFAELLNSNVVNDLILLETMMDNMTGRQKDSCMLVRMLALRGLGNIASGSPEKVRKHGAKLLASMVNGMDDKDDPQNLVALEAMSSLSKLLDHLEERDVQSMLLHIAIRIRPFFDSEQPDLRQSSIVLFGNLTKFSQGHCEVFVEQILNGLVTLLLHLQDPKPEVVKACKFALRMCGPNMGCEGLCDMFLNHLQEERSLHYGEFMNNVCKHLMQCYPEMLNRLILTNLFYFKSNWVDIRAAAPMFIGFLVLHVDEEQCQQVDLDQLISALNLLLKDPVPAVRMKVAETLGRLVRIL

Target ORF information:

RefSeq Version	XM_014956147.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax maestro heat-like repeat family member 1 (MROH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956147.1

ORF Insert Sequence:

ATGACGGAGT CACGGATAAA ACGGCTTGCC ACGACGTTGA TGGATGCCAC CACGGATAAG 
GACCCGCTGG TCCAGGAGCA GATCTACAAT GCCCTGTGCT ACCTGGGGGA ATCCGAGCCG 
GAGGAGATCC TCAACTCCTG CGACGAGTAC CTCCGGCAGC ACGACAAGCT GGCCTACCCC 
CATCGGGTGA TCATCCTGAA GGCCATGGAG ACCGTGGTGA AGAACAACAT TGCCCTCTTG 
GACAAGAGCA CGGCGAAGGT CGTTATCTTC CTGGCGTCTA ATGAAATGAC CAAGTCAAAG 
GAGGTGATCC GGGACTGGCA ACAAGCCGCC AGCAGCGTCC TCGTGGCAGT GGGGCAGCGC 
TTCATCAACA AGGTGATGGA GGAGGTGCTC ACCAAGTTTC AGCCAGGGAT CCTGCCCCAC 
TACTTCGTCC TGCAGACGTT CGCCAACCTC TCGGTGTCCA ACGTGTTCGG GATGGTGCCT 
TTCCTCAACT CCATCCTGGG GACCATGCTG CCCATGCTGG GAATGGCCAA GCAGGACCAC 
ATGAAGGCTG TCTTCTGCTA CGCCCTGCAG CACTTCAGCG AGAGCATCCA GGAGTACCTG 
GCCAACTTGG ACAAGGCCCC GGACCCCACC GTCAGGAAGG ACACCTTCTC CAATGAGATC 
TTCAGCGCCT ACGAAGTGCT CTTCAACAGC TGGCTGCAGC ACCGGGAAGC CAAGCTGAGA 
CTGGCCGTGG TGGAGGCTCT GGGACCGATG AGTTACCTGA TGCCCAGCGA AAAGCTGGAG 
GAGCAGCTCC CGAAGCTGAT TCCAGGCATC CTCGCCCTCT ACAAGAAGCA CACGGAGGCC 
TTCTACATAT CCAAGAGCCT CTGCCAGATC CTGGAAGCTT CCGTGAACAT CGGCAGCCGC 
AGCCTGGACG TGCAGCTGGA CTCTCTGCTG GGCACCCTGC ACCCCCAGAT TTGTGCCCCT 
GCGGATCCGT CTGTCCCCCT GACTGTTAAA AATCACACCG AGGTTCTGCG ATGCTTCACC 
GTCCTGGCCT GCTCCTTCCC TGACCGCGTG CTGGCCTTCC TCCTCCCCAA GCTGGAGAGC 
AGCAACGAGA GGACACGGGT GGGGACGCTC CTCATCATGA GGCAGATCAT CAACAGCGCC 
CCTTCTCAAA TGGAGATTAA AAAGCCTTTT ATTCTCTCAT CTATGAAACT CCCTCTGCAA 
GACAGCAATA ATAAGGTGAA GCGGGCGGTG GTGCAGGTGA TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCCGG CGGCGAGGCC ATGATGGAGT TCCTGGTCCG ACAGTGCGCC 
CTGCCCTCCG ACGCGCCCAA AAAGCAGCCG CTGGATGCCG ATGACCTCAC TAGTGACAGC 
GTCCAGAGCA TCAGTGTCAA TACGCTCTTC CTCCTCAGCA CGACCGTTGA CAGGATGAAC 
AATGTTCTCT GGCCGTACCT CCTGGAGTTT GTGACTCCCA TACAGTTCAC CAACGCCCTC 
ACCCCGCTCT GCAAGAGCCT CATGTACTTG GCCACGAAGA AGCAAGAGGA GGGAGAAAAC 
ACCTCCCTCA TCCGTTACGA CCTGAACGCA AACCTTCCTT CGCCCTACGC TCTGACGACG 
AGGCTGCTGG TCGTGTCTTC CCAGCCCTAC GCAGGGGACT GCCGGGGGGT GGCTGCCCTG 
CGGCTGCTCA GCGTCTTGCA TTACAGCGTC CATCCTGCCC TGGACCAGCT CTGGAGCAAG 
CAGGTCCCTC TCTTGGTGGA GCACATAGAA GAGAACACGG AGAAGTCCCT GTCCCGGAAG 
GAGTGGGAAG AGAAGCTGCT GCTGTTCCTC CAGGAGACGC TGGCGGCCGT TGCTGACAAC 
GCCTGGATTT GTCACTTTGT CACCGAGATG TGCCGGCAGC TGAGCAGCTA CAACGGCCTC 
CCGCTGGAGA AGAATTTCCT GTATAAATGC ATTGGGACGA CGCTCGGGGC CTGCACCAGC 
AAAGACCTTG TGCAGAAGCA GCTCCAGGAG CTCTTGGAAA CAGCCAGATA CCAAGAGGAG 
GCAGAAAGGG AGGGGCTTGC TTCCTGCTTT GGGATATGTG CAATAAATCA CCTGGAGGAA 
ACCCTGGCCA AGCTGGAAGA TTTCGTTAGG TCTGATGTCT TCAAGAAATC CGTGGGGCTG 
TTCAGTCTCT TCAAGGACCG TAGTGATAAT GAAGTGGAGA AAATCAAGAG CAGCCTCATC 
CTCTGCTACG GCTACGTCGC TGTCTACGCG CCCAAGGAGC TGGTGCTGGC CAGGATCGAA 
GCAGACATCC TGAAGAACAT CTTCCAGTAC TTCAACACTA AGGTTCTGGG AATAAAGGTA 
GAAACCAAGG ACCTGACCCT GAAGCTGTGC CTCATCCGGA GCATCTGCAT GATCAGCCAA 
GCCATCTGCA ACGGCGTCAA GTGCGGAGAC TTCATCTTCT CCCGCAAAGC CGAGCTCGTG 
GCCCAGATGG TGGAGTTCAT CAAAGCCGAG CCGCTGGAGG CCCTGCGGAC GCCGGTGCGG 
CAGCGAGCCA TGGTCGCCTG CACTTACCTG GTCATCCTGG AGCCCCACCT CAGCGACCCC 
GACAGGACGG AGCTGATGGA CACCTGCCTC GCCAGCGTGC TGGCCCTCCC GCCGCCGGAG 
CTGGCCAAGG AGAGGGAGGA GAACGGAGGG GATGCCCTGC ACAAGGAGAT GCTGTACGGT 
GACACCATGG GTGCCCTTAA AGACCTGCTG AAGAGCCTCC TGCAGAGGAA CTTGACCCCC 
CACGGGCTGC AGGACATGTT TGCGCATTTA GGCCCCTGGA TCAAGTCGAG CAAGGAGCAC 
GAGCGGCAGC GGGCGGTGGA GGTCAGCGCC GCCTTGCTGG ACTTCTACCT CAGCAAGCTG 
AATGTCAGTA CCGTCATCCC CTTCTACAAC CTCGGCGTCC TCATTGCCCT CTTCTCCCCG 
CGCTGCTCGG ACTCCCTGGC CAGCGTCCGC CAACACGCCG TCGACTGCGT CTACTGCCTG 
CTCTACATCC AGCTCTGCTA CGAAGGGTTC GCCCGGGATC ACCGAGATGA GATGGTGGAG 
GGGCTGAAAG CTCTGAAGAA AGGCCTGGAG GAGCCCGATT TCACCGTTCT CTTCCACACC 
TGCAACAACA TCGCCATGGT GATCGGGAAG CGTGTCCCTC CGGACCAGCT GATGAGCCTG 
CTGCTGGCCA TGTTCGAGGG GCTGGCGGAC CCCGATAAGA ACTGCTCCCG AGCAGCCACC 
GTCATGATCA ACTCCCTCCT CAAGGAGCGC GGAGCCGTCT TGCAGGAGAA GGTGCCCGAC 
ATCATGAGCA TCATCCACAG CAAGCTGGAG GAGGTGGACG AGGAGCACAT CCGGAAGGCG 
GCCCAGCAGA CGGTCTACAT CCTGGCCGCC CAGCACAAGA GCGTGGTGGT CTCCAGCCTG 
CTGGGCAGCC CCCTGCCCTT CGACAGCCAC ACCTGCGCCA TGTGGAGGTC CCTGGCCACC 
GAGCCCACCC TCACCTCGCA GATCCTGGAG CAGCTCCTGG AGAAGGTCAA CAGGGACGTC 
CCCTACAAGG AGAGCAAGTC CTTCCCTCTG CTGGGGAGCG GATCCGAGCG CGTGGCCACC 
CCCCTGCCCC TGGCCGCCAC ATGTGCTCTG TATGAGATCA TGTCTGCTGC CGAGTCTGGC 
CCGGCCGTGC TGGGGCTCTA CCCGCCCTTG TTTGTCACCC TCCTGCTGCG CCTCAGCTGC 
ACCGTGGGTG TCCAGCTCCC CAGGAACCTG CAGAGCCGCG AGAGGAGGAG CAGCTCCAGC 
CTCGGCCCCG CTCCCCGCAG CCTCCAGCCC TGCAGCTGCG CCGTGGAGAC GCTGAAGGTG 
ATGCTGGTCC GGGGGGGCAG TGAGGAGGTG GCCAGGGCGG TGGGCAGCGC CGGCGGCTGG 
GAGCTGATGG CCAGTCCAGA GAGGCACCAC GATGGCATCG CCGTGCTGGC CAGGGCCATG 
GCACGCCACG CCGGCCCCCG GCTCCCCCTC ATCGTGAAGA ACCTCATCCC CACCCTCAAC 
AGCGTCTTCG ACAGCCAGAG GGTCACCACC ACCGCCTTCT TCGCCGAGCT CCTCAACAGT 
AACGTGGTGA ATGACCTGAT CCTGCTGGAG ACCATGATGG ACAACATGAC GGGGAGGCAG 
AAGGACTCCT GCATGTTGGT GCGGATGCTG GCGCTGCGGG GACTCGGCAA CATCGCCTCT 
GGCTCGCCCG AAAAGGTGAG GAAGCACGGG GCCAAGCTCC TGGCCTCCAT GGTCAACGGC 
ATGGATGACA AAGATGACCC CCAGAACCTG GTGGCCCTGG AGGCCATGTC CAGCCTCTCC 
AAGCTCCTGG ATCACCTGGA GGAGAGGGAC GTCCAGTCCA TGCTCCTCCA CATCGCCATC 
AGGATCCGGC CCTTCTTCGA CAGCGAGCAG CCCGACCTCC GCCAGTCCTC CATCGTCCTC 
TTCGGCAACC TCACCAAGTT CAGCCAAGGC CACTGCGAGG TCTTCGTTGA GCAGATCCTC 
AACGGGCTGG TGACGCTGCT GCTGCACCTC CAGGACCCAA AGCCAGAGGT GGTCAAAGCC 
TGCAAGTTCG CCCTGCGCAT GTGTGGCCCC AACATGGGCT GCGAGGGGCT CTGCGACATG 
TTCCTCAACC ACCTGCAGGA GGAGAGGAGC CTGCACTACG GGGAGTTCAT GAACAACGTC 
TGCAAACACT TGATGCAGTG CTACCCGGAG ATGCTGAACC GCCTCATCTT AACCAACCTC 
TTCTACTTCA AGAGCAACTG GGTGGACATC CGAGCTGCTG CCCCCATGTT CATCGGCTTC 
CTCGTGCTGC ACGTGGATGA AGAGCAGTGC CAGCAAGTGG ACCTGGACCA GCTCATTTCG 
GCTCTGAACC TGCTCCTGAA GGACCCCGTC CCCGCCGTCC GCATGAAGGT GGCGGAGACG 
TTGGGCCGCC TCGTCCGGAT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_014956148.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811634.1 Calidris pugnax maestro heat-like repeat family member 1 (MROH1), transcript variant X3, mRNA.

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CloneID	OCa75387
Clone ID Related Accession (Same CDS sequence)	XM_014956148.1
Accession Version	XM_014956148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	maestro heat-like repeat-containing protein family member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091040.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGT CACGGATAAA ACGGCTTGCC ACGACGTTGA TGGATGCCAC CACGGATAAG 
GACCCGCTGG TCCAGGAGCA GATCTACAAT GCCCTGTGCT ACCTGGGGGA ATCCGAGCCG 
GAGGAGATCC TCAACTCCTG CGACGAGTAC CTCCGGCAGC ACGACAAGCT GGCCTACCCC 
CATCGGGTGA TCATCCTGAA GGCCATGGAG ACCGTGGTGA AGAACAACAT TGCCCTCTTG 
GACAAGAGCA CGGCGAAGGT CGTTATCTTC CTGGCGTCTA ATGAAATGAC CAAGTCAAAG 
GAGGTGATCC GGGACTGGCA ACAAGCCGCC AGCAGCGTCC TCGTGGCAGT GGGGCAGCGC 
TTCATCAACA AGGTGATGGA GGAGGTGCTC ACCAAGTTTC AGCCAGGGAT CCTGCCCCAC 
TACTTCGTCC TGCAGACGTT CGCCAACCTC TCGGTGTCCA ACGTGTTCGG GATGGTGCCT 
TTCCTCAACT CCATCCTGGG GACCATGCTG CCCATGCTGG GAATGGCCAA GCAGGACCAC 
ATGAAGGCTG TCTTCTGCTA CGCCCTGCAG CACTTCAGCG AGAGCATCCA GGAGTACCTG 
GCCAACTTGG ACAAGGCCCC GGACCCCACC GTCAGGAAGG ACACCTTCTC CAATGAGATC 
TTCAGCGCCT ACGAAGTGCT CTTCAACAGC TGGCTGCAGC ACCGGGAAGC CAAGCTGAGA 
CTGGCCGTGG TGGAGGCTCT GGGACCGATG AGTTACCTGA TGCCCAGCGA AAAGCTGGAG 
GAGCAGCTCC CGAAGCTGAT TCCAGGCATC CTCGCCCTCT ACAAGAAGCA CACGGAGGCC 
TTCTACATAT CCAAGAGCCT CTGCCAGATC CTGGAAGCTT CCGTGAACAT CGGCAGCCGC 
AGCCTGGACG TGCAGCTGGA CTCTCTGCTG GGCACCCTGC ACCCCCAGAT TTGTGCCCCT 
GCGGATCCGT CTGTCCCCCT GACTGTTAAA AATCACACCG AGGTTCTGCG ATGCTTCACC 
GTCCTGGCCT GCTCCTTCCC TGACCGCGTG CTGGCCTTCC TCCTCCCCAA GCTGGAGAGC 
AGCAACGAGA GGACACGGGT GGGGACGCTC CTCATCATGA GGCAGATCAT CAACAGCGCC 
CCTTCTCAAA TGGAGATTAA AAAGCCTTTT ATTCTCTCAT CTATGAAACT CCCTCTGCAA 
GACAGCAATA ATAAGGTGAA GCGGGCGGTG GTGCAGGTGA TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCCGG CGGCGAGGCC ATGATGGAGT TCCTGGTCCG ACAGTGCGCC 
CTGCCCTCCG ACGCGCAGCC CAAAAAGCAG CCGCTGGATG CCGATGACCT CACTAGTGAC 
AGCGTCCAGA GCATCAGTGT CAATACGCTC TTCCTCCTCA GCACGACCGT TGACAGGATG 
AACAATGTTC TCTGGCCGTA CCTCCTGGAG TTTGTGACTC CCATACAGTT CACCAACGCC 
CTCACCCCGC TCTGCAAGAG CCTCATGTAC TTGGCCACGA AGAAGCAAGA GGAGGGAGAA 
AACACCTCCC TCATCCGTTA CGACCTGAAC GCAAACCTTC CTTCGCCCTA CGCTCTGACG 
ACGAGGCTGC TGGTCGTGTC TTCCCAGCCC TACGCAGGGG ACTGCCGGGG GGTGGCTGCC 
CTGCGGCTGC TCAGCGTCTT GCATTACAGC GTCCATCCTG CCCTGGACCA GCTCTGGAGC 
AAGCAGGTCC CTCTCTTGGT GGAGCACATA GAAGAGAACA CGGAGAAGTC CCTGTCCCGG 
AAGGAGTGGG AAGAGAAGCT GCTGCTGTTC CTCCAGGAGA CGCTGGCGGC CGTTGCTGAC 
AACGCCTGGA TTTGTCACTT TGTCACCGAG ATGTGCCGGC AGCTGAGCAG CTACAACGGC 
CTCCCGCTGG AGAAGAATTT CCTGTATAAA TGCATTGGGA CGACGCTCGG GGCCTGCACC 
AGCAAAGACC TTGTGCAGAA GCAGCTCCAG GAGCTCTTGG AAACAGCCAG ATACCAAGAG 
GAGGCAGAAA GGGAGGGGCT TGCTTCCTGC TTTGGGATAT GTGCAATAAA TCACCTGGAG 
GAAACCCTGG CCAAGCTGGA AGATTTCGTT AGGTCTGATG TCTTCAAGAA ATCCGTGGGG 
CTGTTCAGTC TCTTCAAGGA CCGTAGTGAT AATGAAGTGG AGAAAATCAA GAGCAGCCTC 
ATCCTCTGCT ACGGCTACGT CGCTGTCTAC GCGCCCAAGG AGCTGGTGCT GGCCAGGATC 
GAAGCAGACA TCCTGAAGAA CATCTTCCAG TACTTCAACA CTAAGGACCT GACCCTGAAG 
CTGTGCCTCA TCCGGAGCAT CTGCATGATC AGCCAAGCCA TCTGCAACGG CGTCAAGTGC 
GGAGACTTCA TCTTCTCCCG CAAAGCCGAG CTCGTGGCCC AGATGGTGGA GTTCATCAAA 
GCCGAGCCGC TGGAGGCCCT GCGGACGCCG GTGCGGCAGC GAGCCATGGT CGCCTGCACT 
TACCTGGTCA TCCTGGAGCC CCACCTCAGC GACCCCGACA GGACGGAGCT GATGGACACC 
TGCCTCGCCA GCGTGCTGGC CCTCCCGCCG CCGGAGCTGG CCAAGGAGAG GGAGGAGAAC 
GGAGGGGATG CCCTGCACAA GGAGATGCTG TACGGTGACA CCATGGGTGC CCTTAAAGAC 
CTGCTGAAGA GCCTCCTGCA GAGGAACTTG ACCCCCCACG GGCTGCAGGA CATGTTTGCG 
CATTTAGGCC CCTGGATCAA GTCGAGCAAG GAGCACGAGC GGCAGCGGGC GGTGGAGGTC 
AGCGCCGCCT TGCTGGACTT CTACCTCAGC AAGCTGAATG TCAGTACCGT CATCCCCTTC 
TACAACCTCG GCGTCCTCAT TGCCCTCTTC TCCCCGCGCT GCTCGGACTC CCTGGCCAGC 
GTCCGCCAAC ACGCCGTCGA CTGCGTCTAC TGCCTGCTCT ACATCCAGCT CTGCTACGAA 
GGGTTCGCCC GGGATCACCG AGATGAGATG GTGGAGGGGC TGAAAGCTCT GAAGAAAGGC 
CTGGAGGAGC CCGATTTCAC CGTTCTCTTC CACACCTGCA ACAACATCGC CATGGTGATC 
GGGAAGCGTG TCCCTCCGGA CCAGCTGATG AGCCTGCTGC TGGCCATGTT CGAGGGGCTG 
GCGGACCCCG ATAAGAACTG CTCCCGAGCA GCCACCGTCA TGATCAACTC CCTCCTCAAG 
GAGCGCGGAG CCGTCTTGCA GGAGAAGGTG CCCGACATCA TGAGCATCAT CCACAGCAAG 
CTGGAGGAGG TGGACGAGGA GCACATCCGG AAGGCGGCCC AGCAGACGGT CTACATCCTG 
GCCGCCCAGC ACAAGAGCGT GGTGGTCTCC AGCCTGCTGG GCAGCCCCCT GCCCTTCGAC 
AGCCACACCT GCGCCATGTG GAGGTCCCTG GCCACCGAGC CCACCCTCAC CTCGCAGATC 
CTGGAGCAGC TCCTGGAGAA GGTCAACAGG GACGTCCCCT ACAAGGAGAG CAAGTCCTTC 
CCTCTGCTGG GGAGCGGATC CGAGCGCGTG GCCACCCCCC TGCCCCTGGC CGCCACATGT 
GCTCTGTATG AGATCATGTC TGCTGCCGAG TCTGGCCCGG CCGTGCTGGG GCTCTACCCG 
CCCTTGTTTG TCACCCTCCT GCTGCGCCTC AGCTGCACCG TGGGTGTCCA GCTCCCCAGG 
AACCTGCAGA GCCGCGAGAG GAGGAGCAGC TCCAGCCTCG GCCCCGCTCC CCGCAGCCTC 
CAGCCCTGCA GCTGCGCCGT GGAGACGCTG AAGGTGATGC TGGTCCGGGG GGGCAGTGAG 
GAGGTGGCCA GGGCGGTGGG CAGCGCCGGC GGCTGGGAGC TGATGGCCAG TCCAGAGAGG 
CACCACGATG GCATCGCCGT GCTGGCCAGG GCCATGGCAC GCCACGCCGG CCCCCGGCTC 
CCCCTCATCG TGAAGAACCT CATCCCCACC CTCAACAGCG TCTTCGACAG CCAGAGGGTC 
ACCACCACCG CCTTCTTCGC CGAGCTCCTC AACAGTAACG TGGTGAATGA CCTGATCCTG 
CTGGAGACCA TGATGGACAA CATGACGGGG AGGCAGAAGG ACTCCTGCAT GTTGGTGCGG 
ATGCTGGCGC TGCGGGGACT CGGCAACATC GCCTCTGGCT CGCCCGAAAA GGTGAGGAAG 
CACGGGGCCA AGCTCCTGGC CTCCATGGTC AACGGCATGG ATGACAAAGA TGACCCCCAG 
AACCTGGTGG CCCTGGAGGC CATGTCCAGC CTCTCCAAGC TCCTGGATCA CCTGGAGGAG 
AGGGACGTCC AGTCCATGCT CCTCCACATC GCCATCAGGA TCCGGCCCTT CTTCGACAGC 
GAGCAGCCCG ACCTCCGCCA GTCCTCCATC GTCCTCTTCG GCAACCTCAC CAAGTTCAGC 
CAAGGCCACT GCGAGGTCTT CGTTGAGCAG ATCCTCAACG GGCTGGTGAC GCTGCTGCTG 
CACCTCCAGG ACCCAAAGCC AGAGGTGGTC AAAGCCTGCA AGTTCGCCCT GCGCATGTGT 
GGCCCCAACA TGGGCTGCGA GGGGCTCTGC GACATGTTCC TCAACCACCT GCAGGAGGAG 
AGGAGCCTGC ACTACGGGGA GTTCATGAAC AACGTCTGCA AACACTTGAT GCAGTGCTAC 
CCGGAGATGC TGAACCGCCT CATCTTAACC AACCTCTTCT ACTTCAAGAG CAACTGGGTG 
GACATCCGAG CTGCTGCCCC CATGTTCATC GGCTTCCTCG TGCTGCACGT GGATGAAGAG 
CAGTGCCAGC AAGTGGACCT GGACCAGCTC ATTTCGGCTC TGAACCTGCT CCTGAAGGAC 
CCCGTCCCCG CCGTCCGCAT GAAGGTGGCG GAGACGTTGG GCCGCCTCGT CCGGATCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014811634.1
CDS	357..5279
Translation	MTESRIKRLATTLMDATTDKDPLVQEQIYNALCYLGESEPEEILNSCDEYLRQHDKLAYPHRVIILKAMETVVKNNIALLDKSTAKVVIFLASNEMTKSKEVIRDWQQAASSVLVAVGQRFINKVMEEVLTKFQPGILPHYFVLQTFANLSVSNVFGMVPFLNSILGTMLPMLGMAKQDHMKAVFCYALQHFSESIQEYLANLDKAPDPTVRKDTFSNEIFSAYEVLFNSWLQHREAKLRLAVVEALGPMSYLMPSEKLEEQLPKLIPGILALYKKHTEAFYISKSLCQILEASVNIGSRSLDVQLDSLLGTLHPQICAPADPSVPLTVKNHTEVLRCFTVLACSFPDRVLAFLLPKLESSNERTRVGTLLIMRQIINSAPSQMEIKKPFILSSMKLPLQDSNNKVKRAVVQVISAMAHHGYLEQPGGEAMMEFLVRQCALPSDAQPKKQPLDADDLTSDSVQSISVNTLFLLSTTVDRMNNVLWPYLLEFVTPIQFTNALTPLCKSLMYLATKKQEEGENTSLIRYDLNANLPSPYALTTRLLVVSSQPYAGDCRGVAALRLLSVLHYSVHPALDQLWSKQVPLLVEHIEENTEKSLSRKEWEEKLLLFLQETLAAVADNAWICHFVTEMCRQLSSYNGLPLEKNFLYKCIGTTLGACTSKDLVQKQLQELLETARYQEEAEREGLASCFGICAINHLEETLAKLEDFVRSDVFKKSVGLFSLFKDRSDNEVEKIKSSLILCYGYVAVYAPKELVLARIEADILKNIFQYFNTKDLTLKLCLIRSICMISQAICNGVKCGDFIFSRKAELVAQMVEFIKAEPLEALRTPVRQRAMVACTYLVILEPHLSDPDRTELMDTCLASVLALPPPELAKEREENGGDALHKEMLYGDTMGALKDLLKSLLQRNLTPHGLQDMFAHLGPWIKSSKEHERQRAVEVSAALLDFYLSKLNVSTVIPFYNLGVLIALFSPRCSDSLASVRQHAVDCVYCLLYIQLCYEGFARDHRDEMVEGLKALKKGLEEPDFTVLFHTCNNIAMVIGKRVPPDQLMSLLLAMFEGLADPDKNCSRAATVMINSLLKERGAVLQEKVPDIMSIIHSKLEEVDEEHIRKAAQQTVYILAAQHKSVVVSSLLGSPLPFDSHTCAMWRSLATEPTLTSQILEQLLEKVNRDVPYKESKSFPLLGSGSERVATPLPLAATCALYEIMSAAESGPAVLGLYPPLFVTLLLRLSCTVGVQLPRNLQSRERRSSSSLGPAPRSLQPCSCAVETLKVMLVRGGSEEVARAVGSAGGWELMASPERHHDGIAVLARAMARHAGPRLPLIVKNLIPTLNSVFDSQRVTTTAFFAELLNSNVVNDLILLETMMDNMTGRQKDSCMLVRMLALRGLGNIASGSPEKVRKHGAKLLASMVNGMDDKDDPQNLVALEAMSSLSKLLDHLEERDVQSMLLHIAIRIRPFFDSEQPDLRQSSIVLFGNLTKFSQGHCEVFVEQILNGLVTLLLHLQDPKPEVVKACKFALRMCGPNMGCEGLCDMFLNHLQEERSLHYGEFMNNVCKHLMQCYPEMLNRLILTNLFYFKSNWVDIRAAAPMFIGFLVLHVDEEQCQQVDLDQLISALNLLLKDPVPAVRMKVAETLGRLVRIL

Target ORF information:

RefSeq Version	XM_014956148.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax maestro heat-like repeat family member 1 (MROH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956148.1

ORF Insert Sequence:

ATGACGGAGT CACGGATAAA ACGGCTTGCC ACGACGTTGA TGGATGCCAC CACGGATAAG 
GACCCGCTGG TCCAGGAGCA GATCTACAAT GCCCTGTGCT ACCTGGGGGA ATCCGAGCCG 
GAGGAGATCC TCAACTCCTG CGACGAGTAC CTCCGGCAGC ACGACAAGCT GGCCTACCCC 
CATCGGGTGA TCATCCTGAA GGCCATGGAG ACCGTGGTGA AGAACAACAT TGCCCTCTTG 
GACAAGAGCA CGGCGAAGGT CGTTATCTTC CTGGCGTCTA ATGAAATGAC CAAGTCAAAG 
GAGGTGATCC GGGACTGGCA ACAAGCCGCC AGCAGCGTCC TCGTGGCAGT GGGGCAGCGC 
TTCATCAACA AGGTGATGGA GGAGGTGCTC ACCAAGTTTC AGCCAGGGAT CCTGCCCCAC 
TACTTCGTCC TGCAGACGTT CGCCAACCTC TCGGTGTCCA ACGTGTTCGG GATGGTGCCT 
TTCCTCAACT CCATCCTGGG GACCATGCTG CCCATGCTGG GAATGGCCAA GCAGGACCAC 
ATGAAGGCTG TCTTCTGCTA CGCCCTGCAG CACTTCAGCG AGAGCATCCA GGAGTACCTG 
GCCAACTTGG ACAAGGCCCC GGACCCCACC GTCAGGAAGG ACACCTTCTC CAATGAGATC 
TTCAGCGCCT ACGAAGTGCT CTTCAACAGC TGGCTGCAGC ACCGGGAAGC CAAGCTGAGA 
CTGGCCGTGG TGGAGGCTCT GGGACCGATG AGTTACCTGA TGCCCAGCGA AAAGCTGGAG 
GAGCAGCTCC CGAAGCTGAT TCCAGGCATC CTCGCCCTCT ACAAGAAGCA CACGGAGGCC 
TTCTACATAT CCAAGAGCCT CTGCCAGATC CTGGAAGCTT CCGTGAACAT CGGCAGCCGC 
AGCCTGGACG TGCAGCTGGA CTCTCTGCTG GGCACCCTGC ACCCCCAGAT TTGTGCCCCT 
GCGGATCCGT CTGTCCCCCT GACTGTTAAA AATCACACCG AGGTTCTGCG ATGCTTCACC 
GTCCTGGCCT GCTCCTTCCC TGACCGCGTG CTGGCCTTCC TCCTCCCCAA GCTGGAGAGC 
AGCAACGAGA GGACACGGGT GGGGACGCTC CTCATCATGA GGCAGATCAT CAACAGCGCC 
CCTTCTCAAA TGGAGATTAA AAAGCCTTTT ATTCTCTCAT CTATGAAACT CCCTCTGCAA 
GACAGCAATA ATAAGGTGAA GCGGGCGGTG GTGCAGGTGA TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCCGG CGGCGAGGCC ATGATGGAGT TCCTGGTCCG ACAGTGCGCC 
CTGCCCTCCG ACGCGCAGCC CAAAAAGCAG CCGCTGGATG CCGATGACCT CACTAGTGAC 
AGCGTCCAGA GCATCAGTGT CAATACGCTC TTCCTCCTCA GCACGACCGT TGACAGGATG 
AACAATGTTC TCTGGCCGTA CCTCCTGGAG TTTGTGACTC CCATACAGTT CACCAACGCC 
CTCACCCCGC TCTGCAAGAG CCTCATGTAC TTGGCCACGA AGAAGCAAGA GGAGGGAGAA 
AACACCTCCC TCATCCGTTA CGACCTGAAC GCAAACCTTC CTTCGCCCTA CGCTCTGACG 
ACGAGGCTGC TGGTCGTGTC TTCCCAGCCC TACGCAGGGG ACTGCCGGGG GGTGGCTGCC 
CTGCGGCTGC TCAGCGTCTT GCATTACAGC GTCCATCCTG CCCTGGACCA GCTCTGGAGC 
AAGCAGGTCC CTCTCTTGGT GGAGCACATA GAAGAGAACA CGGAGAAGTC CCTGTCCCGG 
AAGGAGTGGG AAGAGAAGCT GCTGCTGTTC CTCCAGGAGA CGCTGGCGGC CGTTGCTGAC 
AACGCCTGGA TTTGTCACTT TGTCACCGAG ATGTGCCGGC AGCTGAGCAG CTACAACGGC 
CTCCCGCTGG AGAAGAATTT CCTGTATAAA TGCATTGGGA CGACGCTCGG GGCCTGCACC 
AGCAAAGACC TTGTGCAGAA GCAGCTCCAG GAGCTCTTGG AAACAGCCAG ATACCAAGAG 
GAGGCAGAAA GGGAGGGGCT TGCTTCCTGC TTTGGGATAT GTGCAATAAA TCACCTGGAG 
GAAACCCTGG CCAAGCTGGA AGATTTCGTT AGGTCTGATG TCTTCAAGAA ATCCGTGGGG 
CTGTTCAGTC TCTTCAAGGA CCGTAGTGAT AATGAAGTGG AGAAAATCAA GAGCAGCCTC 
ATCCTCTGCT ACGGCTACGT CGCTGTCTAC GCGCCCAAGG AGCTGGTGCT GGCCAGGATC 
GAAGCAGACA TCCTGAAGAA CATCTTCCAG TACTTCAACA CTAAGGACCT GACCCTGAAG 
CTGTGCCTCA TCCGGAGCAT CTGCATGATC AGCCAAGCCA TCTGCAACGG CGTCAAGTGC 
GGAGACTTCA TCTTCTCCCG CAAAGCCGAG CTCGTGGCCC AGATGGTGGA GTTCATCAAA 
GCCGAGCCGC TGGAGGCCCT GCGGACGCCG GTGCGGCAGC GAGCCATGGT CGCCTGCACT 
TACCTGGTCA TCCTGGAGCC CCACCTCAGC GACCCCGACA GGACGGAGCT GATGGACACC 
TGCCTCGCCA GCGTGCTGGC CCTCCCGCCG CCGGAGCTGG CCAAGGAGAG GGAGGAGAAC 
GGAGGGGATG CCCTGCACAA GGAGATGCTG TACGGTGACA CCATGGGTGC CCTTAAAGAC 
CTGCTGAAGA GCCTCCTGCA GAGGAACTTG ACCCCCCACG GGCTGCAGGA CATGTTTGCG 
CATTTAGGCC CCTGGATCAA GTCGAGCAAG GAGCACGAGC GGCAGCGGGC GGTGGAGGTC 
AGCGCCGCCT TGCTGGACTT CTACCTCAGC AAGCTGAATG TCAGTACCGT CATCCCCTTC 
TACAACCTCG GCGTCCTCAT TGCCCTCTTC TCCCCGCGCT GCTCGGACTC CCTGGCCAGC 
GTCCGCCAAC ACGCCGTCGA CTGCGTCTAC TGCCTGCTCT ACATCCAGCT CTGCTACGAA 
GGGTTCGCCC GGGATCACCG AGATGAGATG GTGGAGGGGC TGAAAGCTCT GAAGAAAGGC 
CTGGAGGAGC CCGATTTCAC CGTTCTCTTC CACACCTGCA ACAACATCGC CATGGTGATC 
GGGAAGCGTG TCCCTCCGGA CCAGCTGATG AGCCTGCTGC TGGCCATGTT CGAGGGGCTG 
GCGGACCCCG ATAAGAACTG CTCCCGAGCA GCCACCGTCA TGATCAACTC CCTCCTCAAG 
GAGCGCGGAG CCGTCTTGCA GGAGAAGGTG CCCGACATCA TGAGCATCAT CCACAGCAAG 
CTGGAGGAGG TGGACGAGGA GCACATCCGG AAGGCGGCCC AGCAGACGGT CTACATCCTG 
GCCGCCCAGC ACAAGAGCGT GGTGGTCTCC AGCCTGCTGG GCAGCCCCCT GCCCTTCGAC 
AGCCACACCT GCGCCATGTG GAGGTCCCTG GCCACCGAGC CCACCCTCAC CTCGCAGATC 
CTGGAGCAGC TCCTGGAGAA GGTCAACAGG GACGTCCCCT ACAAGGAGAG CAAGTCCTTC 
CCTCTGCTGG GGAGCGGATC CGAGCGCGTG GCCACCCCCC TGCCCCTGGC CGCCACATGT 
GCTCTGTATG AGATCATGTC TGCTGCCGAG TCTGGCCCGG CCGTGCTGGG GCTCTACCCG 
CCCTTGTTTG TCACCCTCCT GCTGCGCCTC AGCTGCACCG TGGGTGTCCA GCTCCCCAGG 
AACCTGCAGA GCCGCGAGAG GAGGAGCAGC TCCAGCCTCG GCCCCGCTCC CCGCAGCCTC 
CAGCCCTGCA GCTGCGCCGT GGAGACGCTG AAGGTGATGC TGGTCCGGGG GGGCAGTGAG 
GAGGTGGCCA GGGCGGTGGG CAGCGCCGGC GGCTGGGAGC TGATGGCCAG TCCAGAGAGG 
CACCACGATG GCATCGCCGT GCTGGCCAGG GCCATGGCAC GCCACGCCGG CCCCCGGCTC 
CCCCTCATCG TGAAGAACCT CATCCCCACC CTCAACAGCG TCTTCGACAG CCAGAGGGTC 
ACCACCACCG CCTTCTTCGC CGAGCTCCTC AACAGTAACG TGGTGAATGA CCTGATCCTG 
CTGGAGACCA TGATGGACAA CATGACGGGG AGGCAGAAGG ACTCCTGCAT GTTGGTGCGG 
ATGCTGGCGC TGCGGGGACT CGGCAACATC GCCTCTGGCT CGCCCGAAAA GGTGAGGAAG 
CACGGGGCCA AGCTCCTGGC CTCCATGGTC AACGGCATGG ATGACAAAGA TGACCCCCAG 
AACCTGGTGG CCCTGGAGGC CATGTCCAGC CTCTCCAAGC TCCTGGATCA CCTGGAGGAG 
AGGGACGTCC AGTCCATGCT CCTCCACATC GCCATCAGGA TCCGGCCCTT CTTCGACAGC 
GAGCAGCCCG ACCTCCGCCA GTCCTCCATC GTCCTCTTCG GCAACCTCAC CAAGTTCAGC 
CAAGGCCACT GCGAGGTCTT CGTTGAGCAG ATCCTCAACG GGCTGGTGAC GCTGCTGCTG 
CACCTCCAGG ACCCAAAGCC AGAGGTGGTC AAAGCCTGCA AGTTCGCCCT GCGCATGTGT 
GGCCCCAACA TGGGCTGCGA GGGGCTCTGC GACATGTTCC TCAACCACCT GCAGGAGGAG 
AGGAGCCTGC ACTACGGGGA GTTCATGAAC AACGTCTGCA AACACTTGAT GCAGTGCTAC 
CCGGAGATGC TGAACCGCCT CATCTTAACC AACCTCTTCT ACTTCAAGAG CAACTGGGTG 
GACATCCGAG CTGCTGCCCC CATGTTCATC GGCTTCCTCG TGCTGCACGT GGATGAAGAG 
CAGTGCCAGC AAGTGGACCT GGACCAGCTC ATTTCGGCTC TGAACCTGCT CCTGAAGGAC 
CCCGTCCCCG CCGTCCGCAT GAAGGTGGCG GAGACGTTGG GCCGCCTCGT CCGGATCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MROH1 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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