USP54 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP54
Entrez Gene ID	106896362
Full Name	ubiquitin specific peptidase 54
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014956314.1	XP_014811800.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_014956315.1	XP_014811801.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
XM_014956317.1	XP_014811803.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
XM_014956316.1	XP_014811802.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
XM_014956313.1	XP_014811799.1	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1

Pathways from BioSystems
Gene Ontology

The following USP54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa75521	XM_014956314.1 Latest version!	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa75521	XM_014956313.1 Latest version!	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa75521	XM_014956315.1 Latest version!	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa75522	XM_014956316.1 Latest version!	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa75523	XM_014956317.1 Latest version!	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP54 ( XM_014956314.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811800.1 Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.

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CloneID	OCa75521
Clone ID Related Accession (Same CDS sequence)	XM_014956314.1 , XM_014956315.1 , XM_014956313.1
Accession Version	XM_014956314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGTG GGTCGAGGGA ATGTACAGGG CATGTTTACT 
CCACGAAATA CAACCTCAAT AGCACCCAGT AAAGGTCTCA GCAACGAGCC GGGGCAGAAC 
AGTTGCTTCC TGAATAGTGC GCTGCAGGTT CTCTGGCACC TGGACATTTT TCGCCGCAGT 
TTCCGGCAAA TCACAACGCA CAAATGTATG GGTGATTCTT GCATCTTCTG TGCTCTTAAG 
AGTATCTTCA ACCAGTTCCA GTGTAGCAGC GAGAAGGTGC TGCCTTCTGA TGCCCTGCGC 
ACGGCTCTTG CCAAGACGTT TCAGGATGAG CAACGCTTCC AGCTGGGCAT CATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CCTGTTAATG CGAATTCACT TCCACATTGC AGATGAGACA 
AAGGAGGATA TTTGCACTGC ACCACACTGT GTTTCCCATC AGAAGTTTGC TATGACCTTG 
TTTGAGCAGT GTGTTTGTAC CAGCTGTGGT GCCACCTCTG ACCCTTTACC TTTCATCCAG 
ATGGTACATT ATATTTCCAC AACCTCACTC TGCAATCAAG CTATTTGTAT GCTGGAGAGA 
CGAGAGAAAC CCACTCCAGA TATGTTTGGA GAGCTACTAC AGAATGCCAG CACTATGGGA 
GACCTGCGAA ACTGTCCGAG TAACTGCGGG GAAAAGATCC GTATTCGCCG TGTGCTGATG 
AACTCTCCCC AGATCATCAC CATTGGCTTG GTTTGGGACT CGGACCACTC TGATCTGGCT 
GAGGATGTGA TTCACAGTCT GGGCACTTGC CTTAAACTGG GAGATCTCTT CTTCAGAGTA 
ACTGATGACA GAGCAAAGCA CTCAGAGCTG TATTTGGTGG GGATGATCTG TTACTATGGA 
AAACACTATT CTACCTTCTT CTTCCAAACT AAAATCCGCA AGTGGATGTA CTTTGATGAT 
GCTCATGTCA AAGAGATCGG TCCAAAGTGG AAAGATGTGG TGACGAAGTG CATTAAGGGT 
CACTACCAGC CTTTGCTGCT GCTGTACGCA GATCCCAGAG GAACTCCAGT GTCAACACAA 
GACTTGCCCC CTCAAGTGGA TTTACAGCAA TACAGCAGGA CTTGCTATGA TAGCGAAGAC 
TCGGGGAGAG AGCCTTCCAT CTCGAGTGAC ACCAGGACAG ACTCCTCTAC AGACAGCTAC 
CCTTATAAAC AGGCACACTA TGAGTCTGTG GTCAGTCACT TCTCCTCCGA CTCACAGGGG 
ACTGTCATCT ACAATGTGGA GAACGATGCA GCTTCACAAA GCAGCAGGGA CACAGGGCAC 
CTGACTGACA GCGAGTGCAA TCAGAGGCTG GTTTCCAAAA AGAGCTCACT GGCAGATCGC 
AAGAGGAGCT CTAGCCGGTC TCGTCGAAAA GGAGATGAGG CTCAGTCGTC AGGATACCAC 
AGTGAAGGAG AAACCTTGAA GGAGAAACAA GCCCCCAGAA CTGCCCCCAA ACCACCCAGC 
AGCACCAGCA GGCTGAGGGA ATTTAAAGAG ACAGTGAGCA ATATGATCCA CAGCAGACCA 
CAGCAGATTT CTCAAACTGT CCAGAATTCC CCTCGGGGAG GGAGCAATGT GGATCAGGCA 
GAAACACGAC CCTCAAAAAG CCTCTCTGCT CACTCTCGAG ACTGGGAAGT GGAGAGCACC 
AGTAGCGAGT CCAAGTCCAG TTCCTCCAGC AGGTACCGGC CAACCTGGAG ACCCAAAAGG 
GAATCTCTGA ATATAGACAG CATCTTCAGT AAGGACAAGA GGAAACACTG TGGCTACACT 
CAGCTTAGCC CCTTCACTGA AGAAGCAGGT AAAGAGCTTA ATGAGAACGA GGGGAAGGAA 
CACGCTGGTC AAGAAACGAG ATCAAGCCAG TCAAATGTCA GATACAAGCG TGGAGTGTTA 
GGTCGGGGCA CCCAGCATCA CGTGGTGGAT CAACATCCTC ACCTCATCCA GAGGATGGAG 
TCTGGCTACG AAAGCAGCGA ACGCAACAGC AACAGCCCCG TTAGCCTGGA CATGCCCTTG 
TCTGAAAGCT CAAGCACTCA CAGGGACGTC CATATGAAGC GAACGGGTGG ATTGGTTCCT 
GCCTGGCGTA ACATCCCCAA GTCTCACAGT AGCAGCATCT TGGAAGTGGA GTCTAATTCA 
TCAGCTGGGA GCTGGGCAAA CAGTGCACAC ACCCTTGGAA ACAGTGGGGA GATGTTTGCC 
TCTTCAAAGA GCGAGCTGGA TGAATTGCAA GAGGAGGTGG CAAGGAGAGC ACATGAGCAA 
GAGCTACGCA GAAAAAGAGA AAAGGAAATG GAGGCAGCAA TGGGCTTTAA CCCCCGTCCC 
AGCAGGTTCA TGGATCTCGA TGAACTGCAG AATCAGGGGA GGAGTGATGG CTTTGAGAAG 
TCGATGCAGG AGGCAGACTC AATCTTTGAA GAGTCGCTGA ATCAGGAGCA GAAGGGGGAT 
TGTGCTGCAG CTTTGGCTCT CTGTAACGAA GCTATATCTA AACTAAGACT TGCCATGCAT 
GATGCCAGCG CTAGCACTCA CAGCAGAGCC CTAGTCGATA AGAAGCTGCA AATCAGTATC 
CGAAAAGCCC GGAGCCTCCA GGATCGCATG CAGCACCAAC AACCATCGCA GCCGCTGCCG 
CCGCCAACCT GCCACCCACC CCAGGGTGGC ACTGGGGCCC AGTCTACAAG TGAACAGACT 
GGCCCGCTCC AAGTACTGCT GAGCCAGGAG GTACCACTGG AACCCAACAA AGAAGTGGAA 
TTTGGGTCCA GTTCTTTCTT CCACCCACCT GCTTCCTGCC ATGAATTGCA CTCATCATTA 
TATCCAGAGT CTTCCTCCTT GCGCCCTTCC GAAAGCAATC CATCCAACAG GATCTCTCCA 
AACTTCCAGT CTGCTTCTGC TGATTTTCAT GACCAAGTTC CCTCTTTTCC CTATAGGCAA 
GGGTTGGCAG AGAGGTCTGC GAGAACTGAG AACGATGCCC ACCGTAGTGT GGAATTTGCT 
GACCCCGCAG CCACAGGGCC CAAAGACTTT AGGGAATGCT GTAGTAGCAG CAAGTTAGAA 
GAGGTTCATA ACGTAACGCC TGTTCTCACA CCTCAGTACA AACCCAAGGC TAACACAATA 
AACTCGGGGT CTTTGCCTGC ACTGTTTCCT TGTCCACATC CACCACAAGC AGCATCTCCC 
GAAAAGAATG GCCAGCACAG TCCTTGTTCC AAACTTCCAA GCTCTCCAGT GCGGCCCAGC 
ATTGATCATT TAGGGGCCTA TCATGCAGTG ACCCCTGCAA CTTCATCCCC TACACAACAG 
TGCTACTTGG ATCCTTTGCA GAAAACCAAT AAGTACTCCA GAGCAAACAG CCACGAGGTT 
TTATTACCCT CACAGGATGA ATATGGCACA GCAGAAGGTT GCAAATCTGA GGCTTTTAGT 
CCAAAGGGAC ACGTTCGCTC CTTGGCAGAG CAATTTCAGA AAATGCATGC AGTCTCCCAG 
AGGAAAGGTA CTCATGAAAA AGGCTGGATT GCTGCAGTGT GTCAGGATGA GAAGTCTCCA 
AAGTTAACAC AAAAATCTTT CATCAACCCT TCATCCTCTG GTTACAGACA GCTAATCCAT 
CAAAACCAAG AGACCAAAAC CCAGTCTTCT GAGAAATCAC ACTCAACTGT GGATATTAGG 
GACGGGGTAG TTTCTTTGGA GGGTTTTAGT GCCCAACATG TTGCTTCTAA GAGTATTTGT 
TCTCACAGTG AAGAGCTGTG TAGAAGAGGT GGACAGACTA TAGCTGACCC TGCACCCTAC 
CTGGAAAGGC ACTGTCATGA TGGAGGAATG AAGCAGGTGG ATGATGGAGC TACTGGTAGC 
GTGGTTGCCT CTATGCCTGT GGACCGTTGG GTGGATAATG TTACTAGGTA TTACAGTTCT 
CAGAAGGCTA ATAAGAATAC TGAATGGCTT CCTGTACCTG GGAGGAGTCC ACCCCTTTCT 
GATCAGAGAG CGGTTGGAGA TGACTTGAGC AGGATCCCGG TACATCTGCA CCCACAGTGG 
AATCAAGACA CAGAACAGGA GCTCTCAGAA CTGGAATCCC TCTATCAGAC TAGTCTGCAG 
GCATCTCAGG CCACTAGGCC TTTCTTGGGA AGACAGGACA GTGCTAGGTA TCCATTTGAC 
CAATCAGTCT CTGCGAACCT GAACGTGAGG AAGCTGCATG TTACAGCTGG CATGGGGCTC 
TCCAAAACCC CCACGGCAGA GATCGAGCGC AGTCTGTGTG GATCCGCTGT CTCTTCTGTC 
TCCAAGGTCA CGTACACCAG AGACCATAGC AGGGAACCAG GAGAGGAGGA AATGTACAGT 
GCAGAGAATT TCCGTCGCAT CGCTCGCAGT CTCAGTGGAA CTGTCATCTC AAACAGAGAA 
GAGACCTTGG TCTCTTCTCA CAGTTTTGAA ACACCAAATA TGAGGAAAAT GCCAGTGGAC 
ACCAGCCACC GTTCTTCCAG CTCCACTTCC CTTCCTTTCC CCCATGATCC TCCTGTGTTT 
CCCTTTGATC CCCAGCACAA CCCAACCCAG GTGCAGCACC TACCCCATGA TGTACTGGTG 
CCCAGCATGG TGGGCAAGGC ACGTAAACCC TCAGGAGATC AGAAGAACAT CATCCAGAAA 
CTTTTGGTTG TGCCTGACAG GGGTGAAGCT TTCCAAGGTG GAGACTACCC AGGTGTGGCA 
CTGAGCTACG GGAGTCTCCC TTGTGCACCC AAAGGCACCC TCTCCCAGGG GCAAAAGCCC 
CTCGTTAATC CATATTTAGA AGGTGGAGCA CCAAGTGTCT CTGGCCACCG GCCCAACGCG 
AGCCACGCTG CCAGGCAAGC GGCCACCTTG CCGGAGAAAC GAGCCACGCT CTGGGGGGGA 
CACGTTGGCC AACCTGGCAA GAGTTTACAG GAGGGCTTCC TCCCGCAGCC TTCGCAGCAT 
CCAGTGCATG AATCCTACCC CGTGGGTTGC AGGCTGCCGG CCGGCACAGA CTACAGCACT 
TTAAGAACAC CACGCGAGCC TTCTTGGGAT CCCGGTGCCT CTCAAGGTTG TCCCGTGCCC 
CCTTCTTGCC TGAAGGCCCC GGGTCCAAGG AGAGTTGACA TGCCCCCAGA AGAAGACTGG 
CGGCAGAACA GCTACACCCC TCAGCCTGGC AGAAGGCGGA TGCTGCCCAC GCGTGGGATG 
GAGGGGGCTT TTTCTCCTGC CTCTGAGGCA CACAGAAATA TGCTGGCGAC TGGCACCATG 
CAGAATAACG GGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014811800.1
CDS	773..6010
Translation	MSWKRNYFSVGRGNVQGMFTPRNTTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQITTHKCMGDSCIFCALKSIFNQFQCSSEKVLPSDALRTALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAPHCVSHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPTPDMFGELLQNASTMGDLRNCPSNCGEKIRIRRVLMNSPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVSTQDLPPQVDLQQYSRTCYDSEDSGREPSISSDTRTDSSTDSYPYKQAHYESVVSHFSSDSQGTVIYNVENDAASQSSRDTGHLTDSECNQRLVSKKSSLADRKRSSSRSRRKGDEAQSSGYHSEGETLKEKQAPRTAPKPPSSTSRLREFKETVSNMIHSRPQQISQTVQNSPRGGSNVDQAETRPSKSLSAHSRDWEVESTSSESKSSSSSRYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFTEEAGKELNENEGKEHAGQETRSSQSNVRYKRGVLGRGTQHHVVDQHPHLIQRMESGYESSERNSNSPVSLDMPLSESSSTHRDVHMKRTGGLVPAWRNIPKSHSSSILEVESNSSAGSWANSAHTLGNSGEMFASSKSELDELQEEVARRAHEQELRRKREKEMEAAMGFNPRPSRFMDLDELQNQGRSDGFEKSMQEADSIFEESLNQEQKGDCAAALALCNEAISKLRLAMHDASASTHSRALVDKKLQISIRKARSLQDRMQHQQPSQPLPPPTCHPPQGGTGAQSTSEQTGPLQVLLSQEVPLEPNKEVEFGSSSFFHPPASCHELHSSLYPESSSLRPSESNPSNRISPNFQSASADFHDQVPSFPYRQGLAERSARTENDAHRSVEFADPAATGPKDFRECCSSSKLEEVHNVTPVLTPQYKPKANTINSGSLPALFPCPHPPQAASPEKNGQHSPCSKLPSSPVRPSIDHLGAYHAVTPATSSPTQQCYLDPLQKTNKYSRANSHEVLLPSQDEYGTAEGCKSEAFSPKGHVRSLAEQFQKMHAVSQRKGTHEKGWIAAVCQDEKSPKLTQKSFINPSSSGYRQLIHQNQETKTQSSEKSHSTVDIRDGVVSLEGFSAQHVASKSICSHSEELCRRGGQTIADPAPYLERHCHDGGMKQVDDGATGSVVASMPVDRWVDNVTRYYSSQKANKNTEWLPVPGRSPPLSDQRAVGDDLSRIPVHLHPQWNQDTEQELSELESLYQTSLQASQATRPFLGRQDSARYPFDQSVSANLNVRKLHVTAGMGLSKTPTAEIERSLCGSAVSSVSKVTYTRDHSREPGEEEMYSAENFRRIARSLSGTVISNREETLVSSHSFETPNMRKMPVDTSHRSSSSTSLPFPHDPPVFPFDPQHNPTQVQHLPHDVLVPSMVGKARKPSGDQKNIIQKLLVVPDRGEAFQGGDYPGVALSYGSLPCAPKGTLSQGQKPLVNPYLEGGAPSVSGHRPNASHAARQAATLPEKRATLWGGHVGQPGKSLQEGFLPQPSQHPVHESYPVGCRLPAGTDYSTLRTPREPSWDPGASQGCPVPPSCLKAPGPRRVDMPPEEDWRQNSYTPQPGRRRMLPTRGMEGAFSPASEAHRNMLATGTMQNNGW

Target ORF information:

RefSeq Version	XM_014956314.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956314.1

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGTG GGTCGAGGGA ATGTACAGGG CATGTTTACT 
CCACGAAATA CAACCTCAAT AGCACCCAGT AAAGGTCTCA GCAACGAGCC GGGGCAGAAC 
AGTTGCTTCC TGAATAGTGC GCTGCAGGTT CTCTGGCACC TGGACATTTT TCGCCGCAGT 
TTCCGGCAAA TCACAACGCA CAAATGTATG GGTGATTCTT GCATCTTCTG TGCTCTTAAG 
AGTATCTTCA ACCAGTTCCA GTGTAGCAGC GAGAAGGTGC TGCCTTCTGA TGCCCTGCGC 
ACGGCTCTTG CCAAGACGTT TCAGGATGAG CAACGCTTCC AGCTGGGCAT CATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CCTGTTAATG CGAATTCACT TCCACATTGC AGATGAGACA 
AAGGAGGATA TTTGCACTGC ACCACACTGT GTTTCCCATC AGAAGTTTGC TATGACCTTG 
TTTGAGCAGT GTGTTTGTAC CAGCTGTGGT GCCACCTCTG ACCCTTTACC TTTCATCCAG 
ATGGTACATT ATATTTCCAC AACCTCACTC TGCAATCAAG CTATTTGTAT GCTGGAGAGA 
CGAGAGAAAC CCACTCCAGA TATGTTTGGA GAGCTACTAC AGAATGCCAG CACTATGGGA 
GACCTGCGAA ACTGTCCGAG TAACTGCGGG GAAAAGATCC GTATTCGCCG TGTGCTGATG 
AACTCTCCCC AGATCATCAC CATTGGCTTG GTTTGGGACT CGGACCACTC TGATCTGGCT 
GAGGATGTGA TTCACAGTCT GGGCACTTGC CTTAAACTGG GAGATCTCTT CTTCAGAGTA 
ACTGATGACA GAGCAAAGCA CTCAGAGCTG TATTTGGTGG GGATGATCTG TTACTATGGA 
AAACACTATT CTACCTTCTT CTTCCAAACT AAAATCCGCA AGTGGATGTA CTTTGATGAT 
GCTCATGTCA AAGAGATCGG TCCAAAGTGG AAAGATGTGG TGACGAAGTG CATTAAGGGT 
CACTACCAGC CTTTGCTGCT GCTGTACGCA GATCCCAGAG GAACTCCAGT GTCAACACAA 
GACTTGCCCC CTCAAGTGGA TTTACAGCAA TACAGCAGGA CTTGCTATGA TAGCGAAGAC 
TCGGGGAGAG AGCCTTCCAT CTCGAGTGAC ACCAGGACAG ACTCCTCTAC AGACAGCTAC 
CCTTATAAAC AGGCACACTA TGAGTCTGTG GTCAGTCACT TCTCCTCCGA CTCACAGGGG 
ACTGTCATCT ACAATGTGGA GAACGATGCA GCTTCACAAA GCAGCAGGGA CACAGGGCAC 
CTGACTGACA GCGAGTGCAA TCAGAGGCTG GTTTCCAAAA AGAGCTCACT GGCAGATCGC 
AAGAGGAGCT CTAGCCGGTC TCGTCGAAAA GGAGATGAGG CTCAGTCGTC AGGATACCAC 
AGTGAAGGAG AAACCTTGAA GGAGAAACAA GCCCCCAGAA CTGCCCCCAA ACCACCCAGC 
AGCACCAGCA GGCTGAGGGA ATTTAAAGAG ACAGTGAGCA ATATGATCCA CAGCAGACCA 
CAGCAGATTT CTCAAACTGT CCAGAATTCC CCTCGGGGAG GGAGCAATGT GGATCAGGCA 
GAAACACGAC CCTCAAAAAG CCTCTCTGCT CACTCTCGAG ACTGGGAAGT GGAGAGCACC 
AGTAGCGAGT CCAAGTCCAG TTCCTCCAGC AGGTACCGGC CAACCTGGAG ACCCAAAAGG 
GAATCTCTGA ATATAGACAG CATCTTCAGT AAGGACAAGA GGAAACACTG TGGCTACACT 
CAGCTTAGCC CCTTCACTGA AGAAGCAGGT AAAGAGCTTA ATGAGAACGA GGGGAAGGAA 
CACGCTGGTC AAGAAACGAG ATCAAGCCAG TCAAATGTCA GATACAAGCG TGGAGTGTTA 
GGTCGGGGCA CCCAGCATCA CGTGGTGGAT CAACATCCTC ACCTCATCCA GAGGATGGAG 
TCTGGCTACG AAAGCAGCGA ACGCAACAGC AACAGCCCCG TTAGCCTGGA CATGCCCTTG 
TCTGAAAGCT CAAGCACTCA CAGGGACGTC CATATGAAGC GAACGGGTGG ATTGGTTCCT 
GCCTGGCGTA ACATCCCCAA GTCTCACAGT AGCAGCATCT TGGAAGTGGA GTCTAATTCA 
TCAGCTGGGA GCTGGGCAAA CAGTGCACAC ACCCTTGGAA ACAGTGGGGA GATGTTTGCC 
TCTTCAAAGA GCGAGCTGGA TGAATTGCAA GAGGAGGTGG CAAGGAGAGC ACATGAGCAA 
GAGCTACGCA GAAAAAGAGA AAAGGAAATG GAGGCAGCAA TGGGCTTTAA CCCCCGTCCC 
AGCAGGTTCA TGGATCTCGA TGAACTGCAG AATCAGGGGA GGAGTGATGG CTTTGAGAAG 
TCGATGCAGG AGGCAGACTC AATCTTTGAA GAGTCGCTGA ATCAGGAGCA GAAGGGGGAT 
TGTGCTGCAG CTTTGGCTCT CTGTAACGAA GCTATATCTA AACTAAGACT TGCCATGCAT 
GATGCCAGCG CTAGCACTCA CAGCAGAGCC CTAGTCGATA AGAAGCTGCA AATCAGTATC 
CGAAAAGCCC GGAGCCTCCA GGATCGCATG CAGCACCAAC AACCATCGCA GCCGCTGCCG 
CCGCCAACCT GCCACCCACC CCAGGGTGGC ACTGGGGCCC AGTCTACAAG TGAACAGACT 
GGCCCGCTCC AAGTACTGCT GAGCCAGGAG GTACCACTGG AACCCAACAA AGAAGTGGAA 
TTTGGGTCCA GTTCTTTCTT CCACCCACCT GCTTCCTGCC ATGAATTGCA CTCATCATTA 
TATCCAGAGT CTTCCTCCTT GCGCCCTTCC GAAAGCAATC CATCCAACAG GATCTCTCCA 
AACTTCCAGT CTGCTTCTGC TGATTTTCAT GACCAAGTTC CCTCTTTTCC CTATAGGCAA 
GGGTTGGCAG AGAGGTCTGC GAGAACTGAG AACGATGCCC ACCGTAGTGT GGAATTTGCT 
GACCCCGCAG CCACAGGGCC CAAAGACTTT AGGGAATGCT GTAGTAGCAG CAAGTTAGAA 
GAGGTTCATA ACGTAACGCC TGTTCTCACA CCTCAGTACA AACCCAAGGC TAACACAATA 
AACTCGGGGT CTTTGCCTGC ACTGTTTCCT TGTCCACATC CACCACAAGC AGCATCTCCC 
GAAAAGAATG GCCAGCACAG TCCTTGTTCC AAACTTCCAA GCTCTCCAGT GCGGCCCAGC 
ATTGATCATT TAGGGGCCTA TCATGCAGTG ACCCCTGCAA CTTCATCCCC TACACAACAG 
TGCTACTTGG ATCCTTTGCA GAAAACCAAT AAGTACTCCA GAGCAAACAG CCACGAGGTT 
TTATTACCCT CACAGGATGA ATATGGCACA GCAGAAGGTT GCAAATCTGA GGCTTTTAGT 
CCAAAGGGAC ACGTTCGCTC CTTGGCAGAG CAATTTCAGA AAATGCATGC AGTCTCCCAG 
AGGAAAGGTA CTCATGAAAA AGGCTGGATT GCTGCAGTGT GTCAGGATGA GAAGTCTCCA 
AAGTTAACAC AAAAATCTTT CATCAACCCT TCATCCTCTG GTTACAGACA GCTAATCCAT 
CAAAACCAAG AGACCAAAAC CCAGTCTTCT GAGAAATCAC ACTCAACTGT GGATATTAGG 
GACGGGGTAG TTTCTTTGGA GGGTTTTAGT GCCCAACATG TTGCTTCTAA GAGTATTTGT 
TCTCACAGTG AAGAGCTGTG TAGAAGAGGT GGACAGACTA TAGCTGACCC TGCACCCTAC 
CTGGAAAGGC ACTGTCATGA TGGAGGAATG AAGCAGGTGG ATGATGGAGC TACTGGTAGC 
GTGGTTGCCT CTATGCCTGT GGACCGTTGG GTGGATAATG TTACTAGGTA TTACAGTTCT 
CAGAAGGCTA ATAAGAATAC TGAATGGCTT CCTGTACCTG GGAGGAGTCC ACCCCTTTCT 
GATCAGAGAG CGGTTGGAGA TGACTTGAGC AGGATCCCGG TACATCTGCA CCCACAGTGG 
AATCAAGACA CAGAACAGGA GCTCTCAGAA CTGGAATCCC TCTATCAGAC TAGTCTGCAG 
GCATCTCAGG CCACTAGGCC TTTCTTGGGA AGACAGGACA GTGCTAGGTA TCCATTTGAC 
CAATCAGTCT CTGCGAACCT GAACGTGAGG AAGCTGCATG TTACAGCTGG CATGGGGCTC 
TCCAAAACCC CCACGGCAGA GATCGAGCGC AGTCTGTGTG GATCCGCTGT CTCTTCTGTC 
TCCAAGGTCA CGTACACCAG AGACCATAGC AGGGAACCAG GAGAGGAGGA AATGTACAGT 
GCAGAGAATT TCCGTCGCAT CGCTCGCAGT CTCAGTGGAA CTGTCATCTC AAACAGAGAA 
GAGACCTTGG TCTCTTCTCA CAGTTTTGAA ACACCAAATA TGAGGAAAAT GCCAGTGGAC 
ACCAGCCACC GTTCTTCCAG CTCCACTTCC CTTCCTTTCC CCCATGATCC TCCTGTGTTT 
CCCTTTGATC CCCAGCACAA CCCAACCCAG GTGCAGCACC TACCCCATGA TGTACTGGTG 
CCCAGCATGG TGGGCAAGGC ACGTAAACCC TCAGGAGATC AGAAGAACAT CATCCAGAAA 
CTTTTGGTTG TGCCTGACAG GGGTGAAGCT TTCCAAGGTG GAGACTACCC AGGTGTGGCA 
CTGAGCTACG GGAGTCTCCC TTGTGCACCC AAAGGCACCC TCTCCCAGGG GCAAAAGCCC 
CTCGTTAATC CATATTTAGA AGGTGGAGCA CCAAGTGTCT CTGGCCACCG GCCCAACGCG 
AGCCACGCTG CCAGGCAAGC GGCCACCTTG CCGGAGAAAC GAGCCACGCT CTGGGGGGGA 
CACGTTGGCC AACCTGGCAA GAGTTTACAG GAGGGCTTCC TCCCGCAGCC TTCGCAGCAT 
CCAGTGCATG AATCCTACCC CGTGGGTTGC AGGCTGCCGG CCGGCACAGA CTACAGCACT 
TTAAGAACAC CACGCGAGCC TTCTTGGGAT CCCGGTGCCT CTCAAGGTTG TCCCGTGCCC 
CCTTCTTGCC TGAAGGCCCC GGGTCCAAGG AGAGTTGACA TGCCCCCAGA AGAAGACTGG 
CGGCAGAACA GCTACACCCC TCAGCCTGGC AGAAGGCGGA TGCTGCCCAC GCGTGGGATG 
GAGGGGGCTT TTTCTCCTGC CTCTGAGGCA CACAGAAATA TGCTGGCGAC TGGCACCATG 
CAGAATAACG GGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_014956315.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811801.1 Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.

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CloneID	OCa75521
Clone ID Related Accession (Same CDS sequence)	XM_014956314.1 , XM_014956315.1 , XM_014956313.1
Accession Version	XM_014956315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGTG GGTCGAGGGA ATGTACAGGG CATGTTTACT 
CCACGAAATA CAACCTCAAT AGCACCCAGT AAAGGTCTCA GCAACGAGCC GGGGCAGAAC 
AGTTGCTTCC TGAATAGTGC GCTGCAGGTT CTCTGGCACC TGGACATTTT TCGCCGCAGT 
TTCCGGCAAA TCACAACGCA CAAATGTATG GGTGATTCTT GCATCTTCTG TGCTCTTAAG 
AGTATCTTCA ACCAGTTCCA GTGTAGCAGC GAGAAGGTGC TGCCTTCTGA TGCCCTGCGC 
ACGGCTCTTG CCAAGACGTT TCAGGATGAG CAACGCTTCC AGCTGGGCAT CATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CCTGTTAATG CGAATTCACT TCCACATTGC AGATGAGACA 
AAGGAGGATA TTTGCACTGC ACCACACTGT GTTTCCCATC AGAAGTTTGC TATGACCTTG 
TTTGAGCAGT GTGTTTGTAC CAGCTGTGGT GCCACCTCTG ACCCTTTACC TTTCATCCAG 
ATGGTACATT ATATTTCCAC AACCTCACTC TGCAATCAAG CTATTTGTAT GCTGGAGAGA 
CGAGAGAAAC CCACTCCAGA TATGTTTGGA GAGCTACTAC AGAATGCCAG CACTATGGGA 
GACCTGCGAA ACTGTCCGAG TAACTGCGGG GAAAAGATCC GTATTCGCCG TGTGCTGATG 
AACTCTCCCC AGATCATCAC CATTGGCTTG GTTTGGGACT CGGACCACTC TGATCTGGCT 
GAGGATGTGA TTCACAGTCT GGGCACTTGC CTTAAACTGG GAGATCTCTT CTTCAGAGTA 
ACTGATGACA GAGCAAAGCA CTCAGAGCTG TATTTGGTGG GGATGATCTG TTACTATGGA 
AAACACTATT CTACCTTCTT CTTCCAAACT AAAATCCGCA AGTGGATGTA CTTTGATGAT 
GCTCATGTCA AAGAGATCGG TCCAAAGTGG AAAGATGTGG TGACGAAGTG CATTAAGGGT 
CACTACCAGC CTTTGCTGCT GCTGTACGCA GATCCCAGAG GAACTCCAGT GTCAACACAA 
GACTTGCCCC CTCAAGTGGA TTTACAGCAA TACAGCAGGA CTTGCTATGA TAGCGAAGAC 
TCGGGGAGAG AGCCTTCCAT CTCGAGTGAC ACCAGGACAG ACTCCTCTAC AGACAGCTAC 
CCTTATAAAC AGGCACACTA TGAGTCTGTG GTCAGTCACT TCTCCTCCGA CTCACAGGGG 
ACTGTCATCT ACAATGTGGA GAACGATGCA GCTTCACAAA GCAGCAGGGA CACAGGGCAC 
CTGACTGACA GCGAGTGCAA TCAGAGGCTG GTTTCCAAAA AGAGCTCACT GGCAGATCGC 
AAGAGGAGCT CTAGCCGGTC TCGTCGAAAA GGAGATGAGG CTCAGTCGTC AGGATACCAC 
AGTGAAGGAG AAACCTTGAA GGAGAAACAA GCCCCCAGAA CTGCCCCCAA ACCACCCAGC 
AGCACCAGCA GGCTGAGGGA ATTTAAAGAG ACAGTGAGCA ATATGATCCA CAGCAGACCA 
CAGCAGATTT CTCAAACTGT CCAGAATTCC CCTCGGGGAG GGAGCAATGT GGATCAGGCA 
GAAACACGAC CCTCAAAAAG CCTCTCTGCT CACTCTCGAG ACTGGGAAGT GGAGAGCACC 
AGTAGCGAGT CCAAGTCCAG TTCCTCCAGC AGGTACCGGC CAACCTGGAG ACCCAAAAGG 
GAATCTCTGA ATATAGACAG CATCTTCAGT AAGGACAAGA GGAAACACTG TGGCTACACT 
CAGCTTAGCC CCTTCACTGA AGAAGCAGGT AAAGAGCTTA ATGAGAACGA GGGGAAGGAA 
CACGCTGGTC AAGAAACGAG ATCAAGCCAG TCAAATGTCA GATACAAGCG TGGAGTGTTA 
GGTCGGGGCA CCCAGCATCA CGTGGTGGAT CAACATCCTC ACCTCATCCA GAGGATGGAG 
TCTGGCTACG AAAGCAGCGA ACGCAACAGC AACAGCCCCG TTAGCCTGGA CATGCCCTTG 
TCTGAAAGCT CAAGCACTCA CAGGGACGTC CATATGAAGC GAACGGGTGG ATTGGTTCCT 
GCCTGGCGTA ACATCCCCAA GTCTCACAGT AGCAGCATCT TGGAAGTGGA GTCTAATTCA 
TCAGCTGGGA GCTGGGCAAA CAGTGCACAC ACCCTTGGAA ACAGTGGGGA GATGTTTGCC 
TCTTCAAAGA GCGAGCTGGA TGAATTGCAA GAGGAGGTGG CAAGGAGAGC ACATGAGCAA 
GAGCTACGCA GAAAAAGAGA AAAGGAAATG GAGGCAGCAA TGGGCTTTAA CCCCCGTCCC 
AGCAGGTTCA TGGATCTCGA TGAACTGCAG AATCAGGGGA GGAGTGATGG CTTTGAGAAG 
TCGATGCAGG AGGCAGACTC AATCTTTGAA GAGTCGCTGA ATCAGGAGCA GAAGGGGGAT 
TGTGCTGCAG CTTTGGCTCT CTGTAACGAA GCTATATCTA AACTAAGACT TGCCATGCAT 
GATGCCAGCG CTAGCACTCA CAGCAGAGCC CTAGTCGATA AGAAGCTGCA AATCAGTATC 
CGAAAAGCCC GGAGCCTCCA GGATCGCATG CAGCACCAAC AACCATCGCA GCCGCTGCCG 
CCGCCAACCT GCCACCCACC CCAGGGTGGC ACTGGGGCCC AGTCTACAAG TGAACAGACT 
GGCCCGCTCC AAGTACTGCT GAGCCAGGAG GTACCACTGG AACCCAACAA AGAAGTGGAA 
TTTGGGTCCA GTTCTTTCTT CCACCCACCT GCTTCCTGCC ATGAATTGCA CTCATCATTA 
TATCCAGAGT CTTCCTCCTT GCGCCCTTCC GAAAGCAATC CATCCAACAG GATCTCTCCA 
AACTTCCAGT CTGCTTCTGC TGATTTTCAT GACCAAGTTC CCTCTTTTCC CTATAGGCAA 
GGGTTGGCAG AGAGGTCTGC GAGAACTGAG AACGATGCCC ACCGTAGTGT GGAATTTGCT 
GACCCCGCAG CCACAGGGCC CAAAGACTTT AGGGAATGCT GTAGTAGCAG CAAGTTAGAA 
GAGGTTCATA ACGTAACGCC TGTTCTCACA CCTCAGTACA AACCCAAGGC TAACACAATA 
AACTCGGGGT CTTTGCCTGC ACTGTTTCCT TGTCCACATC CACCACAAGC AGCATCTCCC 
GAAAAGAATG GCCAGCACAG TCCTTGTTCC AAACTTCCAA GCTCTCCAGT GCGGCCCAGC 
ATTGATCATT TAGGGGCCTA TCATGCAGTG ACCCCTGCAA CTTCATCCCC TACACAACAG 
TGCTACTTGG ATCCTTTGCA GAAAACCAAT AAGTACTCCA GAGCAAACAG CCACGAGGTT 
TTATTACCCT CACAGGATGA ATATGGCACA GCAGAAGGTT GCAAATCTGA GGCTTTTAGT 
CCAAAGGGAC ACGTTCGCTC CTTGGCAGAG CAATTTCAGA AAATGCATGC AGTCTCCCAG 
AGGAAAGGTA CTCATGAAAA AGGCTGGATT GCTGCAGTGT GTCAGGATGA GAAGTCTCCA 
AAGTTAACAC AAAAATCTTT CATCAACCCT TCATCCTCTG GTTACAGACA GCTAATCCAT 
CAAAACCAAG AGACCAAAAC CCAGTCTTCT GAGAAATCAC ACTCAACTGT GGATATTAGG 
GACGGGGTAG TTTCTTTGGA GGGTTTTAGT GCCCAACATG TTGCTTCTAA GAGTATTTGT 
TCTCACAGTG AAGAGCTGTG TAGAAGAGGT GGACAGACTA TAGCTGACCC TGCACCCTAC 
CTGGAAAGGC ACTGTCATGA TGGAGGAATG AAGCAGGTGG ATGATGGAGC TACTGGTAGC 
GTGGTTGCCT CTATGCCTGT GGACCGTTGG GTGGATAATG TTACTAGGTA TTACAGTTCT 
CAGAAGGCTA ATAAGAATAC TGAATGGCTT CCTGTACCTG GGAGGAGTCC ACCCCTTTCT 
GATCAGAGAG CGGTTGGAGA TGACTTGAGC AGGATCCCGG TACATCTGCA CCCACAGTGG 
AATCAAGACA CAGAACAGGA GCTCTCAGAA CTGGAATCCC TCTATCAGAC TAGTCTGCAG 
GCATCTCAGG CCACTAGGCC TTTCTTGGGA AGACAGGACA GTGCTAGGTA TCCATTTGAC 
CAATCAGTCT CTGCGAACCT GAACGTGAGG AAGCTGCATG TTACAGCTGG CATGGGGCTC 
TCCAAAACCC CCACGGCAGA GATCGAGCGC AGTCTGTGTG GATCCGCTGT CTCTTCTGTC 
TCCAAGGTCA CGTACACCAG AGACCATAGC AGGGAACCAG GAGAGGAGGA AATGTACAGT 
GCAGAGAATT TCCGTCGCAT CGCTCGCAGT CTCAGTGGAA CTGTCATCTC AAACAGAGAA 
GAGACCTTGG TCTCTTCTCA CAGTTTTGAA ACACCAAATA TGAGGAAAAT GCCAGTGGAC 
ACCAGCCACC GTTCTTCCAG CTCCACTTCC CTTCCTTTCC CCCATGATCC TCCTGTGTTT 
CCCTTTGATC CCCAGCACAA CCCAACCCAG GTGCAGCACC TACCCCATGA TGTACTGGTG 
CCCAGCATGG TGGGCAAGGC ACGTAAACCC TCAGGAGATC AGAAGAACAT CATCCAGAAA 
CTTTTGGTTG TGCCTGACAG GGGTGAAGCT TTCCAAGGTG GAGACTACCC AGGTGTGGCA 
CTGAGCTACG GGAGTCTCCC TTGTGCACCC AAAGGCACCC TCTCCCAGGG GCAAAAGCCC 
CTCGTTAATC CATATTTAGA AGGTGGAGCA CCAAGTGTCT CTGGCCACCG GCCCAACGCG 
AGCCACGCTG CCAGGCAAGC GGCCACCTTG CCGGAGAAAC GAGCCACGCT CTGGGGGGGA 
CACGTTGGCC AACCTGGCAA GAGTTTACAG GAGGGCTTCC TCCCGCAGCC TTCGCAGCAT 
CCAGTGCATG AATCCTACCC CGTGGGTTGC AGGCTGCCGG CCGGCACAGA CTACAGCACT 
TTAAGAACAC CACGCGAGCC TTCTTGGGAT CCCGGTGCCT CTCAAGGTTG TCCCGTGCCC 
CCTTCTTGCC TGAAGGCCCC GGGTCCAAGG AGAGTTGACA TGCCCCCAGA AGAAGACTGG 
CGGCAGAACA GCTACACCCC TCAGCCTGGC AGAAGGCGGA TGCTGCCCAC GCGTGGGATG 
GAGGGGGCTT TTTCTCCTGC CTCTGAGGCA CACAGAAATA TGCTGGCGAC TGGCACCATG 
CAGAATAACG GGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014811801.1
CDS	781..6018
Translation	MSWKRNYFSVGRGNVQGMFTPRNTTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQITTHKCMGDSCIFCALKSIFNQFQCSSEKVLPSDALRTALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAPHCVSHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPTPDMFGELLQNASTMGDLRNCPSNCGEKIRIRRVLMNSPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVSTQDLPPQVDLQQYSRTCYDSEDSGREPSISSDTRTDSSTDSYPYKQAHYESVVSHFSSDSQGTVIYNVENDAASQSSRDTGHLTDSECNQRLVSKKSSLADRKRSSSRSRRKGDEAQSSGYHSEGETLKEKQAPRTAPKPPSSTSRLREFKETVSNMIHSRPQQISQTVQNSPRGGSNVDQAETRPSKSLSAHSRDWEVESTSSESKSSSSSRYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFTEEAGKELNENEGKEHAGQETRSSQSNVRYKRGVLGRGTQHHVVDQHPHLIQRMESGYESSERNSNSPVSLDMPLSESSSTHRDVHMKRTGGLVPAWRNIPKSHSSSILEVESNSSAGSWANSAHTLGNSGEMFASSKSELDELQEEVARRAHEQELRRKREKEMEAAMGFNPRPSRFMDLDELQNQGRSDGFEKSMQEADSIFEESLNQEQKGDCAAALALCNEAISKLRLAMHDASASTHSRALVDKKLQISIRKARSLQDRMQHQQPSQPLPPPTCHPPQGGTGAQSTSEQTGPLQVLLSQEVPLEPNKEVEFGSSSFFHPPASCHELHSSLYPESSSLRPSESNPSNRISPNFQSASADFHDQVPSFPYRQGLAERSARTENDAHRSVEFADPAATGPKDFRECCSSSKLEEVHNVTPVLTPQYKPKANTINSGSLPALFPCPHPPQAASPEKNGQHSPCSKLPSSPVRPSIDHLGAYHAVTPATSSPTQQCYLDPLQKTNKYSRANSHEVLLPSQDEYGTAEGCKSEAFSPKGHVRSLAEQFQKMHAVSQRKGTHEKGWIAAVCQDEKSPKLTQKSFINPSSSGYRQLIHQNQETKTQSSEKSHSTVDIRDGVVSLEGFSAQHVASKSICSHSEELCRRGGQTIADPAPYLERHCHDGGMKQVDDGATGSVVASMPVDRWVDNVTRYYSSQKANKNTEWLPVPGRSPPLSDQRAVGDDLSRIPVHLHPQWNQDTEQELSELESLYQTSLQASQATRPFLGRQDSARYPFDQSVSANLNVRKLHVTAGMGLSKTPTAEIERSLCGSAVSSVSKVTYTRDHSREPGEEEMYSAENFRRIARSLSGTVISNREETLVSSHSFETPNMRKMPVDTSHRSSSSTSLPFPHDPPVFPFDPQHNPTQVQHLPHDVLVPSMVGKARKPSGDQKNIIQKLLVVPDRGEAFQGGDYPGVALSYGSLPCAPKGTLSQGQKPLVNPYLEGGAPSVSGHRPNASHAARQAATLPEKRATLWGGHVGQPGKSLQEGFLPQPSQHPVHESYPVGCRLPAGTDYSTLRTPREPSWDPGASQGCPVPPSCLKAPGPRRVDMPPEEDWRQNSYTPQPGRRRMLPTRGMEGAFSPASEAHRNMLATGTMQNNGW

Target ORF information:

RefSeq Version	XM_014956315.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956315.1

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGTG GGTCGAGGGA ATGTACAGGG CATGTTTACT 
CCACGAAATA CAACCTCAAT AGCACCCAGT AAAGGTCTCA GCAACGAGCC GGGGCAGAAC 
AGTTGCTTCC TGAATAGTGC GCTGCAGGTT CTCTGGCACC TGGACATTTT TCGCCGCAGT 
TTCCGGCAAA TCACAACGCA CAAATGTATG GGTGATTCTT GCATCTTCTG TGCTCTTAAG 
AGTATCTTCA ACCAGTTCCA GTGTAGCAGC GAGAAGGTGC TGCCTTCTGA TGCCCTGCGC 
ACGGCTCTTG CCAAGACGTT TCAGGATGAG CAACGCTTCC AGCTGGGCAT CATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CCTGTTAATG CGAATTCACT TCCACATTGC AGATGAGACA 
AAGGAGGATA TTTGCACTGC ACCACACTGT GTTTCCCATC AGAAGTTTGC TATGACCTTG 
TTTGAGCAGT GTGTTTGTAC CAGCTGTGGT GCCACCTCTG ACCCTTTACC TTTCATCCAG 
ATGGTACATT ATATTTCCAC AACCTCACTC TGCAATCAAG CTATTTGTAT GCTGGAGAGA 
CGAGAGAAAC CCACTCCAGA TATGTTTGGA GAGCTACTAC AGAATGCCAG CACTATGGGA 
GACCTGCGAA ACTGTCCGAG TAACTGCGGG GAAAAGATCC GTATTCGCCG TGTGCTGATG 
AACTCTCCCC AGATCATCAC CATTGGCTTG GTTTGGGACT CGGACCACTC TGATCTGGCT 
GAGGATGTGA TTCACAGTCT GGGCACTTGC CTTAAACTGG GAGATCTCTT CTTCAGAGTA 
ACTGATGACA GAGCAAAGCA CTCAGAGCTG TATTTGGTGG GGATGATCTG TTACTATGGA 
AAACACTATT CTACCTTCTT CTTCCAAACT AAAATCCGCA AGTGGATGTA CTTTGATGAT 
GCTCATGTCA AAGAGATCGG TCCAAAGTGG AAAGATGTGG TGACGAAGTG CATTAAGGGT 
CACTACCAGC CTTTGCTGCT GCTGTACGCA GATCCCAGAG GAACTCCAGT GTCAACACAA 
GACTTGCCCC CTCAAGTGGA TTTACAGCAA TACAGCAGGA CTTGCTATGA TAGCGAAGAC 
TCGGGGAGAG AGCCTTCCAT CTCGAGTGAC ACCAGGACAG ACTCCTCTAC AGACAGCTAC 
CCTTATAAAC AGGCACACTA TGAGTCTGTG GTCAGTCACT TCTCCTCCGA CTCACAGGGG 
ACTGTCATCT ACAATGTGGA GAACGATGCA GCTTCACAAA GCAGCAGGGA CACAGGGCAC 
CTGACTGACA GCGAGTGCAA TCAGAGGCTG GTTTCCAAAA AGAGCTCACT GGCAGATCGC 
AAGAGGAGCT CTAGCCGGTC TCGTCGAAAA GGAGATGAGG CTCAGTCGTC AGGATACCAC 
AGTGAAGGAG AAACCTTGAA GGAGAAACAA GCCCCCAGAA CTGCCCCCAA ACCACCCAGC 
AGCACCAGCA GGCTGAGGGA ATTTAAAGAG ACAGTGAGCA ATATGATCCA CAGCAGACCA 
CAGCAGATTT CTCAAACTGT CCAGAATTCC CCTCGGGGAG GGAGCAATGT GGATCAGGCA 
GAAACACGAC CCTCAAAAAG CCTCTCTGCT CACTCTCGAG ACTGGGAAGT GGAGAGCACC 
AGTAGCGAGT CCAAGTCCAG TTCCTCCAGC AGGTACCGGC CAACCTGGAG ACCCAAAAGG 
GAATCTCTGA ATATAGACAG CATCTTCAGT AAGGACAAGA GGAAACACTG TGGCTACACT 
CAGCTTAGCC CCTTCACTGA AGAAGCAGGT AAAGAGCTTA ATGAGAACGA GGGGAAGGAA 
CACGCTGGTC AAGAAACGAG ATCAAGCCAG TCAAATGTCA GATACAAGCG TGGAGTGTTA 
GGTCGGGGCA CCCAGCATCA CGTGGTGGAT CAACATCCTC ACCTCATCCA GAGGATGGAG 
TCTGGCTACG AAAGCAGCGA ACGCAACAGC AACAGCCCCG TTAGCCTGGA CATGCCCTTG 
TCTGAAAGCT CAAGCACTCA CAGGGACGTC CATATGAAGC GAACGGGTGG ATTGGTTCCT 
GCCTGGCGTA ACATCCCCAA GTCTCACAGT AGCAGCATCT TGGAAGTGGA GTCTAATTCA 
TCAGCTGGGA GCTGGGCAAA CAGTGCACAC ACCCTTGGAA ACAGTGGGGA GATGTTTGCC 
TCTTCAAAGA GCGAGCTGGA TGAATTGCAA GAGGAGGTGG CAAGGAGAGC ACATGAGCAA 
GAGCTACGCA GAAAAAGAGA AAAGGAAATG GAGGCAGCAA TGGGCTTTAA CCCCCGTCCC 
AGCAGGTTCA TGGATCTCGA TGAACTGCAG AATCAGGGGA GGAGTGATGG CTTTGAGAAG 
TCGATGCAGG AGGCAGACTC AATCTTTGAA GAGTCGCTGA ATCAGGAGCA GAAGGGGGAT 
TGTGCTGCAG CTTTGGCTCT CTGTAACGAA GCTATATCTA AACTAAGACT TGCCATGCAT 
GATGCCAGCG CTAGCACTCA CAGCAGAGCC CTAGTCGATA AGAAGCTGCA AATCAGTATC 
CGAAAAGCCC GGAGCCTCCA GGATCGCATG CAGCACCAAC AACCATCGCA GCCGCTGCCG 
CCGCCAACCT GCCACCCACC CCAGGGTGGC ACTGGGGCCC AGTCTACAAG TGAACAGACT 
GGCCCGCTCC AAGTACTGCT GAGCCAGGAG GTACCACTGG AACCCAACAA AGAAGTGGAA 
TTTGGGTCCA GTTCTTTCTT CCACCCACCT GCTTCCTGCC ATGAATTGCA CTCATCATTA 
TATCCAGAGT CTTCCTCCTT GCGCCCTTCC GAAAGCAATC CATCCAACAG GATCTCTCCA 
AACTTCCAGT CTGCTTCTGC TGATTTTCAT GACCAAGTTC CCTCTTTTCC CTATAGGCAA 
GGGTTGGCAG AGAGGTCTGC GAGAACTGAG AACGATGCCC ACCGTAGTGT GGAATTTGCT 
GACCCCGCAG CCACAGGGCC CAAAGACTTT AGGGAATGCT GTAGTAGCAG CAAGTTAGAA 
GAGGTTCATA ACGTAACGCC TGTTCTCACA CCTCAGTACA AACCCAAGGC TAACACAATA 
AACTCGGGGT CTTTGCCTGC ACTGTTTCCT TGTCCACATC CACCACAAGC AGCATCTCCC 
GAAAAGAATG GCCAGCACAG TCCTTGTTCC AAACTTCCAA GCTCTCCAGT GCGGCCCAGC 
ATTGATCATT TAGGGGCCTA TCATGCAGTG ACCCCTGCAA CTTCATCCCC TACACAACAG 
TGCTACTTGG ATCCTTTGCA GAAAACCAAT AAGTACTCCA GAGCAAACAG CCACGAGGTT 
TTATTACCCT CACAGGATGA ATATGGCACA GCAGAAGGTT GCAAATCTGA GGCTTTTAGT 
CCAAAGGGAC ACGTTCGCTC CTTGGCAGAG CAATTTCAGA AAATGCATGC AGTCTCCCAG 
AGGAAAGGTA CTCATGAAAA AGGCTGGATT GCTGCAGTGT GTCAGGATGA GAAGTCTCCA 
AAGTTAACAC AAAAATCTTT CATCAACCCT TCATCCTCTG GTTACAGACA GCTAATCCAT 
CAAAACCAAG AGACCAAAAC CCAGTCTTCT GAGAAATCAC ACTCAACTGT GGATATTAGG 
GACGGGGTAG TTTCTTTGGA GGGTTTTAGT GCCCAACATG TTGCTTCTAA GAGTATTTGT 
TCTCACAGTG AAGAGCTGTG TAGAAGAGGT GGACAGACTA TAGCTGACCC TGCACCCTAC 
CTGGAAAGGC ACTGTCATGA TGGAGGAATG AAGCAGGTGG ATGATGGAGC TACTGGTAGC 
GTGGTTGCCT CTATGCCTGT GGACCGTTGG GTGGATAATG TTACTAGGTA TTACAGTTCT 
CAGAAGGCTA ATAAGAATAC TGAATGGCTT CCTGTACCTG GGAGGAGTCC ACCCCTTTCT 
GATCAGAGAG CGGTTGGAGA TGACTTGAGC AGGATCCCGG TACATCTGCA CCCACAGTGG 
AATCAAGACA CAGAACAGGA GCTCTCAGAA CTGGAATCCC TCTATCAGAC TAGTCTGCAG 
GCATCTCAGG CCACTAGGCC TTTCTTGGGA AGACAGGACA GTGCTAGGTA TCCATTTGAC 
CAATCAGTCT CTGCGAACCT GAACGTGAGG AAGCTGCATG TTACAGCTGG CATGGGGCTC 
TCCAAAACCC CCACGGCAGA GATCGAGCGC AGTCTGTGTG GATCCGCTGT CTCTTCTGTC 
TCCAAGGTCA CGTACACCAG AGACCATAGC AGGGAACCAG GAGAGGAGGA AATGTACAGT 
GCAGAGAATT TCCGTCGCAT CGCTCGCAGT CTCAGTGGAA CTGTCATCTC AAACAGAGAA 
GAGACCTTGG TCTCTTCTCA CAGTTTTGAA ACACCAAATA TGAGGAAAAT GCCAGTGGAC 
ACCAGCCACC GTTCTTCCAG CTCCACTTCC CTTCCTTTCC CCCATGATCC TCCTGTGTTT 
CCCTTTGATC CCCAGCACAA CCCAACCCAG GTGCAGCACC TACCCCATGA TGTACTGGTG 
CCCAGCATGG TGGGCAAGGC ACGTAAACCC TCAGGAGATC AGAAGAACAT CATCCAGAAA 
CTTTTGGTTG TGCCTGACAG GGGTGAAGCT TTCCAAGGTG GAGACTACCC AGGTGTGGCA 
CTGAGCTACG GGAGTCTCCC TTGTGCACCC AAAGGCACCC TCTCCCAGGG GCAAAAGCCC 
CTCGTTAATC CATATTTAGA AGGTGGAGCA CCAAGTGTCT CTGGCCACCG GCCCAACGCG 
AGCCACGCTG CCAGGCAAGC GGCCACCTTG CCGGAGAAAC GAGCCACGCT CTGGGGGGGA 
CACGTTGGCC AACCTGGCAA GAGTTTACAG GAGGGCTTCC TCCCGCAGCC TTCGCAGCAT 
CCAGTGCATG AATCCTACCC CGTGGGTTGC AGGCTGCCGG CCGGCACAGA CTACAGCACT 
TTAAGAACAC CACGCGAGCC TTCTTGGGAT CCCGGTGCCT CTCAAGGTTG TCCCGTGCCC 
CCTTCTTGCC TGAAGGCCCC GGGTCCAAGG AGAGTTGACA TGCCCCCAGA AGAAGACTGG 
CGGCAGAACA GCTACACCCC TCAGCCTGGC AGAAGGCGGA TGCTGCCCAC GCGTGGGATG 
GAGGGGGCTT TTTCTCCTGC CTCTGAGGCA CACAGAAATA TGCTGGCGAC TGGCACCATG 
CAGAATAACG GGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_014956313.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811799.1 Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.

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CloneID	OCa75521
Clone ID Related Accession (Same CDS sequence)	XM_014956314.1 , XM_014956315.1 , XM_014956313.1
Accession Version	XM_014956313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGTG GGTCGAGGGA ATGTACAGGG CATGTTTACT 
CCACGAAATA CAACCTCAAT AGCACCCAGT AAAGGTCTCA GCAACGAGCC GGGGCAGAAC 
AGTTGCTTCC TGAATAGTGC GCTGCAGGTT CTCTGGCACC TGGACATTTT TCGCCGCAGT 
TTCCGGCAAA TCACAACGCA CAAATGTATG GGTGATTCTT GCATCTTCTG TGCTCTTAAG 
AGTATCTTCA ACCAGTTCCA GTGTAGCAGC GAGAAGGTGC TGCCTTCTGA TGCCCTGCGC 
ACGGCTCTTG CCAAGACGTT TCAGGATGAG CAACGCTTCC AGCTGGGCAT CATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CCTGTTAATG CGAATTCACT TCCACATTGC AGATGAGACA 
AAGGAGGATA TTTGCACTGC ACCACACTGT GTTTCCCATC AGAAGTTTGC TATGACCTTG 
TTTGAGCAGT GTGTTTGTAC CAGCTGTGGT GCCACCTCTG ACCCTTTACC TTTCATCCAG 
ATGGTACATT ATATTTCCAC AACCTCACTC TGCAATCAAG CTATTTGTAT GCTGGAGAGA 
CGAGAGAAAC CCACTCCAGA TATGTTTGGA GAGCTACTAC AGAATGCCAG CACTATGGGA 
GACCTGCGAA ACTGTCCGAG TAACTGCGGG GAAAAGATCC GTATTCGCCG TGTGCTGATG 
AACTCTCCCC AGATCATCAC CATTGGCTTG GTTTGGGACT CGGACCACTC TGATCTGGCT 
GAGGATGTGA TTCACAGTCT GGGCACTTGC CTTAAACTGG GAGATCTCTT CTTCAGAGTA 
ACTGATGACA GAGCAAAGCA CTCAGAGCTG TATTTGGTGG GGATGATCTG TTACTATGGA 
AAACACTATT CTACCTTCTT CTTCCAAACT AAAATCCGCA AGTGGATGTA CTTTGATGAT 
GCTCATGTCA AAGAGATCGG TCCAAAGTGG AAAGATGTGG TGACGAAGTG CATTAAGGGT 
CACTACCAGC CTTTGCTGCT GCTGTACGCA GATCCCAGAG GAACTCCAGT GTCAACACAA 
GACTTGCCCC CTCAAGTGGA TTTACAGCAA TACAGCAGGA CTTGCTATGA TAGCGAAGAC 
TCGGGGAGAG AGCCTTCCAT CTCGAGTGAC ACCAGGACAG ACTCCTCTAC AGACAGCTAC 
CCTTATAAAC AGGCACACTA TGAGTCTGTG GTCAGTCACT TCTCCTCCGA CTCACAGGGG 
ACTGTCATCT ACAATGTGGA GAACGATGCA GCTTCACAAA GCAGCAGGGA CACAGGGCAC 
CTGACTGACA GCGAGTGCAA TCAGAGGCTG GTTTCCAAAA AGAGCTCACT GGCAGATCGC 
AAGAGGAGCT CTAGCCGGTC TCGTCGAAAA GGAGATGAGG CTCAGTCGTC AGGATACCAC 
AGTGAAGGAG AAACCTTGAA GGAGAAACAA GCCCCCAGAA CTGCCCCCAA ACCACCCAGC 
AGCACCAGCA GGCTGAGGGA ATTTAAAGAG ACAGTGAGCA ATATGATCCA CAGCAGACCA 
CAGCAGATTT CTCAAACTGT CCAGAATTCC CCTCGGGGAG GGAGCAATGT GGATCAGGCA 
GAAACACGAC CCTCAAAAAG CCTCTCTGCT CACTCTCGAG ACTGGGAAGT GGAGAGCACC 
AGTAGCGAGT CCAAGTCCAG TTCCTCCAGC AGGTACCGGC CAACCTGGAG ACCCAAAAGG 
GAATCTCTGA ATATAGACAG CATCTTCAGT AAGGACAAGA GGAAACACTG TGGCTACACT 
CAGCTTAGCC CCTTCACTGA AGAAGCAGGT AAAGAGCTTA ATGAGAACGA GGGGAAGGAA 
CACGCTGGTC AAGAAACGAG ATCAAGCCAG TCAAATGTCA GATACAAGCG TGGAGTGTTA 
GGTCGGGGCA CCCAGCATCA CGTGGTGGAT CAACATCCTC ACCTCATCCA GAGGATGGAG 
TCTGGCTACG AAAGCAGCGA ACGCAACAGC AACAGCCCCG TTAGCCTGGA CATGCCCTTG 
TCTGAAAGCT CAAGCACTCA CAGGGACGTC CATATGAAGC GAACGGGTGG ATTGGTTCCT 
GCCTGGCGTA ACATCCCCAA GTCTCACAGT AGCAGCATCT TGGAAGTGGA GTCTAATTCA 
TCAGCTGGGA GCTGGGCAAA CAGTGCACAC ACCCTTGGAA ACAGTGGGGA GATGTTTGCC 
TCTTCAAAGA GCGAGCTGGA TGAATTGCAA GAGGAGGTGG CAAGGAGAGC ACATGAGCAA 
GAGCTACGCA GAAAAAGAGA AAAGGAAATG GAGGCAGCAA TGGGCTTTAA CCCCCGTCCC 
AGCAGGTTCA TGGATCTCGA TGAACTGCAG AATCAGGGGA GGAGTGATGG CTTTGAGAAG 
TCGATGCAGG AGGCAGACTC AATCTTTGAA GAGTCGCTGA ATCAGGAGCA GAAGGGGGAT 
TGTGCTGCAG CTTTGGCTCT CTGTAACGAA GCTATATCTA AACTAAGACT TGCCATGCAT 
GATGCCAGCG CTAGCACTCA CAGCAGAGCC CTAGTCGATA AGAAGCTGCA AATCAGTATC 
CGAAAAGCCC GGAGCCTCCA GGATCGCATG CAGCACCAAC AACCATCGCA GCCGCTGCCG 
CCGCCAACCT GCCACCCACC CCAGGGTGGC ACTGGGGCCC AGTCTACAAG TGAACAGACT 
GGCCCGCTCC AAGTACTGCT GAGCCAGGAG GTACCACTGG AACCCAACAA AGAAGTGGAA 
TTTGGGTCCA GTTCTTTCTT CCACCCACCT GCTTCCTGCC ATGAATTGCA CTCATCATTA 
TATCCAGAGT CTTCCTCCTT GCGCCCTTCC GAAAGCAATC CATCCAACAG GATCTCTCCA 
AACTTCCAGT CTGCTTCTGC TGATTTTCAT GACCAAGTTC CCTCTTTTCC CTATAGGCAA 
GGGTTGGCAG AGAGGTCTGC GAGAACTGAG AACGATGCCC ACCGTAGTGT GGAATTTGCT 
GACCCCGCAG CCACAGGGCC CAAAGACTTT AGGGAATGCT GTAGTAGCAG CAAGTTAGAA 
GAGGTTCATA ACGTAACGCC TGTTCTCACA CCTCAGTACA AACCCAAGGC TAACACAATA 
AACTCGGGGT CTTTGCCTGC ACTGTTTCCT TGTCCACATC CACCACAAGC AGCATCTCCC 
GAAAAGAATG GCCAGCACAG TCCTTGTTCC AAACTTCCAA GCTCTCCAGT GCGGCCCAGC 
ATTGATCATT TAGGGGCCTA TCATGCAGTG ACCCCTGCAA CTTCATCCCC TACACAACAG 
TGCTACTTGG ATCCTTTGCA GAAAACCAAT AAGTACTCCA GAGCAAACAG CCACGAGGTT 
TTATTACCCT CACAGGATGA ATATGGCACA GCAGAAGGTT GCAAATCTGA GGCTTTTAGT 
CCAAAGGGAC ACGTTCGCTC CTTGGCAGAG CAATTTCAGA AAATGCATGC AGTCTCCCAG 
AGGAAAGGTA CTCATGAAAA AGGCTGGATT GCTGCAGTGT GTCAGGATGA GAAGTCTCCA 
AAGTTAACAC AAAAATCTTT CATCAACCCT TCATCCTCTG GTTACAGACA GCTAATCCAT 
CAAAACCAAG AGACCAAAAC CCAGTCTTCT GAGAAATCAC ACTCAACTGT GGATATTAGG 
GACGGGGTAG TTTCTTTGGA GGGTTTTAGT GCCCAACATG TTGCTTCTAA GAGTATTTGT 
TCTCACAGTG AAGAGCTGTG TAGAAGAGGT GGACAGACTA TAGCTGACCC TGCACCCTAC 
CTGGAAAGGC ACTGTCATGA TGGAGGAATG AAGCAGGTGG ATGATGGAGC TACTGGTAGC 
GTGGTTGCCT CTATGCCTGT GGACCGTTGG GTGGATAATG TTACTAGGTA TTACAGTTCT 
CAGAAGGCTA ATAAGAATAC TGAATGGCTT CCTGTACCTG GGAGGAGTCC ACCCCTTTCT 
GATCAGAGAG CGGTTGGAGA TGACTTGAGC AGGATCCCGG TACATCTGCA CCCACAGTGG 
AATCAAGACA CAGAACAGGA GCTCTCAGAA CTGGAATCCC TCTATCAGAC TAGTCTGCAG 
GCATCTCAGG CCACTAGGCC TTTCTTGGGA AGACAGGACA GTGCTAGGTA TCCATTTGAC 
CAATCAGTCT CTGCGAACCT GAACGTGAGG AAGCTGCATG TTACAGCTGG CATGGGGCTC 
TCCAAAACCC CCACGGCAGA GATCGAGCGC AGTCTGTGTG GATCCGCTGT CTCTTCTGTC 
TCCAAGGTCA CGTACACCAG AGACCATAGC AGGGAACCAG GAGAGGAGGA AATGTACAGT 
GCAGAGAATT TCCGTCGCAT CGCTCGCAGT CTCAGTGGAA CTGTCATCTC AAACAGAGAA 
GAGACCTTGG TCTCTTCTCA CAGTTTTGAA ACACCAAATA TGAGGAAAAT GCCAGTGGAC 
ACCAGCCACC GTTCTTCCAG CTCCACTTCC CTTCCTTTCC CCCATGATCC TCCTGTGTTT 
CCCTTTGATC CCCAGCACAA CCCAACCCAG GTGCAGCACC TACCCCATGA TGTACTGGTG 
CCCAGCATGG TGGGCAAGGC ACGTAAACCC TCAGGAGATC AGAAGAACAT CATCCAGAAA 
CTTTTGGTTG TGCCTGACAG GGGTGAAGCT TTCCAAGGTG GAGACTACCC AGGTGTGGCA 
CTGAGCTACG GGAGTCTCCC TTGTGCACCC AAAGGCACCC TCTCCCAGGG GCAAAAGCCC 
CTCGTTAATC CATATTTAGA AGGTGGAGCA CCAAGTGTCT CTGGCCACCG GCCCAACGCG 
AGCCACGCTG CCAGGCAAGC GGCCACCTTG CCGGAGAAAC GAGCCACGCT CTGGGGGGGA 
CACGTTGGCC AACCTGGCAA GAGTTTACAG GAGGGCTTCC TCCCGCAGCC TTCGCAGCAT 
CCAGTGCATG AATCCTACCC CGTGGGTTGC AGGCTGCCGG CCGGCACAGA CTACAGCACT 
TTAAGAACAC CACGCGAGCC TTCTTGGGAT CCCGGTGCCT CTCAAGGTTG TCCCGTGCCC 
CCTTCTTGCC TGAAGGCCCC GGGTCCAAGG AGAGTTGACA TGCCCCCAGA AGAAGACTGG 
CGGCAGAACA GCTACACCCC TCAGCCTGGC AGAAGGCGGA TGCTGCCCAC GCGTGGGATG 
GAGGGGGCTT TTTCTCCTGC CTCTGAGGCA CACAGAAATA TGCTGGCGAC TGGCACCATG 
CAGAATAACG GGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014811799.1
CDS	845..6082
Translation	MSWKRNYFSVGRGNVQGMFTPRNTTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQITTHKCMGDSCIFCALKSIFNQFQCSSEKVLPSDALRTALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAPHCVSHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPTPDMFGELLQNASTMGDLRNCPSNCGEKIRIRRVLMNSPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVSTQDLPPQVDLQQYSRTCYDSEDSGREPSISSDTRTDSSTDSYPYKQAHYESVVSHFSSDSQGTVIYNVENDAASQSSRDTGHLTDSECNQRLVSKKSSLADRKRSSSRSRRKGDEAQSSGYHSEGETLKEKQAPRTAPKPPSSTSRLREFKETVSNMIHSRPQQISQTVQNSPRGGSNVDQAETRPSKSLSAHSRDWEVESTSSESKSSSSSRYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFTEEAGKELNENEGKEHAGQETRSSQSNVRYKRGVLGRGTQHHVVDQHPHLIQRMESGYESSERNSNSPVSLDMPLSESSSTHRDVHMKRTGGLVPAWRNIPKSHSSSILEVESNSSAGSWANSAHTLGNSGEMFASSKSELDELQEEVARRAHEQELRRKREKEMEAAMGFNPRPSRFMDLDELQNQGRSDGFEKSMQEADSIFEESLNQEQKGDCAAALALCNEAISKLRLAMHDASASTHSRALVDKKLQISIRKARSLQDRMQHQQPSQPLPPPTCHPPQGGTGAQSTSEQTGPLQVLLSQEVPLEPNKEVEFGSSSFFHPPASCHELHSSLYPESSSLRPSESNPSNRISPNFQSASADFHDQVPSFPYRQGLAERSARTENDAHRSVEFADPAATGPKDFRECCSSSKLEEVHNVTPVLTPQYKPKANTINSGSLPALFPCPHPPQAASPEKNGQHSPCSKLPSSPVRPSIDHLGAYHAVTPATSSPTQQCYLDPLQKTNKYSRANSHEVLLPSQDEYGTAEGCKSEAFSPKGHVRSLAEQFQKMHAVSQRKGTHEKGWIAAVCQDEKSPKLTQKSFINPSSSGYRQLIHQNQETKTQSSEKSHSTVDIRDGVVSLEGFSAQHVASKSICSHSEELCRRGGQTIADPAPYLERHCHDGGMKQVDDGATGSVVASMPVDRWVDNVTRYYSSQKANKNTEWLPVPGRSPPLSDQRAVGDDLSRIPVHLHPQWNQDTEQELSELESLYQTSLQASQATRPFLGRQDSARYPFDQSVSANLNVRKLHVTAGMGLSKTPTAEIERSLCGSAVSSVSKVTYTRDHSREPGEEEMYSAENFRRIARSLSGTVISNREETLVSSHSFETPNMRKMPVDTSHRSSSSTSLPFPHDPPVFPFDPQHNPTQVQHLPHDVLVPSMVGKARKPSGDQKNIIQKLLVVPDRGEAFQGGDYPGVALSYGSLPCAPKGTLSQGQKPLVNPYLEGGAPSVSGHRPNASHAARQAATLPEKRATLWGGHVGQPGKSLQEGFLPQPSQHPVHESYPVGCRLPAGTDYSTLRTPREPSWDPGASQGCPVPPSCLKAPGPRRVDMPPEEDWRQNSYTPQPGRRRMLPTRGMEGAFSPASEAHRNMLATGTMQNNGW

Target ORF information:

RefSeq Version	XM_014956313.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956313.1

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGTG GGTCGAGGGA ATGTACAGGG CATGTTTACT 
CCACGAAATA CAACCTCAAT AGCACCCAGT AAAGGTCTCA GCAACGAGCC GGGGCAGAAC 
AGTTGCTTCC TGAATAGTGC GCTGCAGGTT CTCTGGCACC TGGACATTTT TCGCCGCAGT 
TTCCGGCAAA TCACAACGCA CAAATGTATG GGTGATTCTT GCATCTTCTG TGCTCTTAAG 
AGTATCTTCA ACCAGTTCCA GTGTAGCAGC GAGAAGGTGC TGCCTTCTGA TGCCCTGCGC 
ACGGCTCTTG CCAAGACGTT TCAGGATGAG CAACGCTTCC AGCTGGGCAT CATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CCTGTTAATG CGAATTCACT TCCACATTGC AGATGAGACA 
AAGGAGGATA TTTGCACTGC ACCACACTGT GTTTCCCATC AGAAGTTTGC TATGACCTTG 
TTTGAGCAGT GTGTTTGTAC CAGCTGTGGT GCCACCTCTG ACCCTTTACC TTTCATCCAG 
ATGGTACATT ATATTTCCAC AACCTCACTC TGCAATCAAG CTATTTGTAT GCTGGAGAGA 
CGAGAGAAAC CCACTCCAGA TATGTTTGGA GAGCTACTAC AGAATGCCAG CACTATGGGA 
GACCTGCGAA ACTGTCCGAG TAACTGCGGG GAAAAGATCC GTATTCGCCG TGTGCTGATG 
AACTCTCCCC AGATCATCAC CATTGGCTTG GTTTGGGACT CGGACCACTC TGATCTGGCT 
GAGGATGTGA TTCACAGTCT GGGCACTTGC CTTAAACTGG GAGATCTCTT CTTCAGAGTA 
ACTGATGACA GAGCAAAGCA CTCAGAGCTG TATTTGGTGG GGATGATCTG TTACTATGGA 
AAACACTATT CTACCTTCTT CTTCCAAACT AAAATCCGCA AGTGGATGTA CTTTGATGAT 
GCTCATGTCA AAGAGATCGG TCCAAAGTGG AAAGATGTGG TGACGAAGTG CATTAAGGGT 
CACTACCAGC CTTTGCTGCT GCTGTACGCA GATCCCAGAG GAACTCCAGT GTCAACACAA 
GACTTGCCCC CTCAAGTGGA TTTACAGCAA TACAGCAGGA CTTGCTATGA TAGCGAAGAC 
TCGGGGAGAG AGCCTTCCAT CTCGAGTGAC ACCAGGACAG ACTCCTCTAC AGACAGCTAC 
CCTTATAAAC AGGCACACTA TGAGTCTGTG GTCAGTCACT TCTCCTCCGA CTCACAGGGG 
ACTGTCATCT ACAATGTGGA GAACGATGCA GCTTCACAAA GCAGCAGGGA CACAGGGCAC 
CTGACTGACA GCGAGTGCAA TCAGAGGCTG GTTTCCAAAA AGAGCTCACT GGCAGATCGC 
AAGAGGAGCT CTAGCCGGTC TCGTCGAAAA GGAGATGAGG CTCAGTCGTC AGGATACCAC 
AGTGAAGGAG AAACCTTGAA GGAGAAACAA GCCCCCAGAA CTGCCCCCAA ACCACCCAGC 
AGCACCAGCA GGCTGAGGGA ATTTAAAGAG ACAGTGAGCA ATATGATCCA CAGCAGACCA 
CAGCAGATTT CTCAAACTGT CCAGAATTCC CCTCGGGGAG GGAGCAATGT GGATCAGGCA 
GAAACACGAC CCTCAAAAAG CCTCTCTGCT CACTCTCGAG ACTGGGAAGT GGAGAGCACC 
AGTAGCGAGT CCAAGTCCAG TTCCTCCAGC AGGTACCGGC CAACCTGGAG ACCCAAAAGG 
GAATCTCTGA ATATAGACAG CATCTTCAGT AAGGACAAGA GGAAACACTG TGGCTACACT 
CAGCTTAGCC CCTTCACTGA AGAAGCAGGT AAAGAGCTTA ATGAGAACGA GGGGAAGGAA 
CACGCTGGTC AAGAAACGAG ATCAAGCCAG TCAAATGTCA GATACAAGCG TGGAGTGTTA 
GGTCGGGGCA CCCAGCATCA CGTGGTGGAT CAACATCCTC ACCTCATCCA GAGGATGGAG 
TCTGGCTACG AAAGCAGCGA ACGCAACAGC AACAGCCCCG TTAGCCTGGA CATGCCCTTG 
TCTGAAAGCT CAAGCACTCA CAGGGACGTC CATATGAAGC GAACGGGTGG ATTGGTTCCT 
GCCTGGCGTA ACATCCCCAA GTCTCACAGT AGCAGCATCT TGGAAGTGGA GTCTAATTCA 
TCAGCTGGGA GCTGGGCAAA CAGTGCACAC ACCCTTGGAA ACAGTGGGGA GATGTTTGCC 
TCTTCAAAGA GCGAGCTGGA TGAATTGCAA GAGGAGGTGG CAAGGAGAGC ACATGAGCAA 
GAGCTACGCA GAAAAAGAGA AAAGGAAATG GAGGCAGCAA TGGGCTTTAA CCCCCGTCCC 
AGCAGGTTCA TGGATCTCGA TGAACTGCAG AATCAGGGGA GGAGTGATGG CTTTGAGAAG 
TCGATGCAGG AGGCAGACTC AATCTTTGAA GAGTCGCTGA ATCAGGAGCA GAAGGGGGAT 
TGTGCTGCAG CTTTGGCTCT CTGTAACGAA GCTATATCTA AACTAAGACT TGCCATGCAT 
GATGCCAGCG CTAGCACTCA CAGCAGAGCC CTAGTCGATA AGAAGCTGCA AATCAGTATC 
CGAAAAGCCC GGAGCCTCCA GGATCGCATG CAGCACCAAC AACCATCGCA GCCGCTGCCG 
CCGCCAACCT GCCACCCACC CCAGGGTGGC ACTGGGGCCC AGTCTACAAG TGAACAGACT 
GGCCCGCTCC AAGTACTGCT GAGCCAGGAG GTACCACTGG AACCCAACAA AGAAGTGGAA 
TTTGGGTCCA GTTCTTTCTT CCACCCACCT GCTTCCTGCC ATGAATTGCA CTCATCATTA 
TATCCAGAGT CTTCCTCCTT GCGCCCTTCC GAAAGCAATC CATCCAACAG GATCTCTCCA 
AACTTCCAGT CTGCTTCTGC TGATTTTCAT GACCAAGTTC CCTCTTTTCC CTATAGGCAA 
GGGTTGGCAG AGAGGTCTGC GAGAACTGAG AACGATGCCC ACCGTAGTGT GGAATTTGCT 
GACCCCGCAG CCACAGGGCC CAAAGACTTT AGGGAATGCT GTAGTAGCAG CAAGTTAGAA 
GAGGTTCATA ACGTAACGCC TGTTCTCACA CCTCAGTACA AACCCAAGGC TAACACAATA 
AACTCGGGGT CTTTGCCTGC ACTGTTTCCT TGTCCACATC CACCACAAGC AGCATCTCCC 
GAAAAGAATG GCCAGCACAG TCCTTGTTCC AAACTTCCAA GCTCTCCAGT GCGGCCCAGC 
ATTGATCATT TAGGGGCCTA TCATGCAGTG ACCCCTGCAA CTTCATCCCC TACACAACAG 
TGCTACTTGG ATCCTTTGCA GAAAACCAAT AAGTACTCCA GAGCAAACAG CCACGAGGTT 
TTATTACCCT CACAGGATGA ATATGGCACA GCAGAAGGTT GCAAATCTGA GGCTTTTAGT 
CCAAAGGGAC ACGTTCGCTC CTTGGCAGAG CAATTTCAGA AAATGCATGC AGTCTCCCAG 
AGGAAAGGTA CTCATGAAAA AGGCTGGATT GCTGCAGTGT GTCAGGATGA GAAGTCTCCA 
AAGTTAACAC AAAAATCTTT CATCAACCCT TCATCCTCTG GTTACAGACA GCTAATCCAT 
CAAAACCAAG AGACCAAAAC CCAGTCTTCT GAGAAATCAC ACTCAACTGT GGATATTAGG 
GACGGGGTAG TTTCTTTGGA GGGTTTTAGT GCCCAACATG TTGCTTCTAA GAGTATTTGT 
TCTCACAGTG AAGAGCTGTG TAGAAGAGGT GGACAGACTA TAGCTGACCC TGCACCCTAC 
CTGGAAAGGC ACTGTCATGA TGGAGGAATG AAGCAGGTGG ATGATGGAGC TACTGGTAGC 
GTGGTTGCCT CTATGCCTGT GGACCGTTGG GTGGATAATG TTACTAGGTA TTACAGTTCT 
CAGAAGGCTA ATAAGAATAC TGAATGGCTT CCTGTACCTG GGAGGAGTCC ACCCCTTTCT 
GATCAGAGAG CGGTTGGAGA TGACTTGAGC AGGATCCCGG TACATCTGCA CCCACAGTGG 
AATCAAGACA CAGAACAGGA GCTCTCAGAA CTGGAATCCC TCTATCAGAC TAGTCTGCAG 
GCATCTCAGG CCACTAGGCC TTTCTTGGGA AGACAGGACA GTGCTAGGTA TCCATTTGAC 
CAATCAGTCT CTGCGAACCT GAACGTGAGG AAGCTGCATG TTACAGCTGG CATGGGGCTC 
TCCAAAACCC CCACGGCAGA GATCGAGCGC AGTCTGTGTG GATCCGCTGT CTCTTCTGTC 
TCCAAGGTCA CGTACACCAG AGACCATAGC AGGGAACCAG GAGAGGAGGA AATGTACAGT 
GCAGAGAATT TCCGTCGCAT CGCTCGCAGT CTCAGTGGAA CTGTCATCTC AAACAGAGAA 
GAGACCTTGG TCTCTTCTCA CAGTTTTGAA ACACCAAATA TGAGGAAAAT GCCAGTGGAC 
ACCAGCCACC GTTCTTCCAG CTCCACTTCC CTTCCTTTCC CCCATGATCC TCCTGTGTTT 
CCCTTTGATC CCCAGCACAA CCCAACCCAG GTGCAGCACC TACCCCATGA TGTACTGGTG 
CCCAGCATGG TGGGCAAGGC ACGTAAACCC TCAGGAGATC AGAAGAACAT CATCCAGAAA 
CTTTTGGTTG TGCCTGACAG GGGTGAAGCT TTCCAAGGTG GAGACTACCC AGGTGTGGCA 
CTGAGCTACG GGAGTCTCCC TTGTGCACCC AAAGGCACCC TCTCCCAGGG GCAAAAGCCC 
CTCGTTAATC CATATTTAGA AGGTGGAGCA CCAAGTGTCT CTGGCCACCG GCCCAACGCG 
AGCCACGCTG CCAGGCAAGC GGCCACCTTG CCGGAGAAAC GAGCCACGCT CTGGGGGGGA 
CACGTTGGCC AACCTGGCAA GAGTTTACAG GAGGGCTTCC TCCCGCAGCC TTCGCAGCAT 
CCAGTGCATG AATCCTACCC CGTGGGTTGC AGGCTGCCGG CCGGCACAGA CTACAGCACT 
TTAAGAACAC CACGCGAGCC TTCTTGGGAT CCCGGTGCCT CTCAAGGTTG TCCCGTGCCC 
CCTTCTTGCC TGAAGGCCCC GGGTCCAAGG AGAGTTGACA TGCCCCCAGA AGAAGACTGG 
CGGCAGAACA GCTACACCCC TCAGCCTGGC AGAAGGCGGA TGCTGCCCAC GCGTGGGATG 
GAGGGGGCTT TTTCTCCTGC CTCTGAGGCA CACAGAAATA TGCTGGCGAC TGGCACCATG 
CAGAATAACG GGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_014956316.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811802.1 Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.

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CloneID	OCa75522
Clone ID Related Accession (Same CDS sequence)	XM_014956316.1
Accession Version	XM_014956316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGA AAAGAAATTA CTTTTCTGTG GGTCGAGGGA ATGTACAGGG CATGTTTACT 
CCACGAAATA CAACCTCAAT AGCACCCAGT AAAGGTCTCA GCAACGAGCC GGGGCAGAAC 
AGTTGCTTCC TGAATAGTGC GCTGCAGGTT CTCTGGCACC TGGACATTTT TCGCCGCAGT 
TTCCGGCAAA TCACAACGCA CAAATGTATG GGTGATTCTT GCATCTTCTG TGCTCTTAAG 
AGTATCTTCA ACCAGTTCCA GTGTAGCAGC GAGAAGGTGC TGCCTTCTGA TGCCCTGCGC 
ACGGCTCTTG CCAAGACGTT TCAGGATGAG CAACGCTTCC AGCTGGGCAT CATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CCTGTTAATG CGAATTCACT TCCACATTGC AGATGAGACA 
AAGGAGGATA TTTGCACTGC ACCACACTGT GTTTCCCATC AGAAGTTTGC TATGACCTTG 
TTTGAGCAGT GTGTTTGTAC CAGCTGTGGT GCCACCTCTG ACCCTTTACC TTTCATCCAG 
ATGGTACATT ATATTTCCAC AACCTCACTC TGCAATCAAG CTATTTGTAT GCTGGAGAGA 
CGAGAGAAAC CCACTCCAGA TATGTTTGGA GAGCTACTAC AGAATGCCAG CACTATGGGA 
GACCTGCGAA ACTGTCCGAG TAACTGCGGG GAAAAGATCC GTATTCGCCG TGTGCTGATG 
AACTCTCCCC AGATCATCAC CATTGGCTTG GTTTGGGACT CGGACCACTC TGATCTGGCT 
GAGGATGTGA TTCACAGTCT GGGCACTTGC CTTAAACTGG GAGATCTCTT CTTCAGAGTA 
ACTGATGACA GAGCAAAGCA CTCAGAGCTG TATTTGGTGG GGATGATCTG TTACTATGGA 
AAACACTATT CTACCTTCTT CTTCCAAACT AAAATCCGCA AGTGGATGTA CTTTGATGAT 
GCTCATGTCA AAGAGATCGG TCCAAAGTGG AAAGATGTGG TGACGAAGTG CATTAAGGGT 
CACTACCAGC CTTTGCTGCT GCTGTACGCA GATCCCAGAG GAACTCCAGT GTCAACACAA 
GACTTGCCCC CTCAAGTGGA TTTACAGCAA TACAGCAGGA CTTGCTATGA TAGCGAAGAC 
TCGGGGAGAG AGCCTTCCAT CTCGAGTGAC ACCAGGACAG ACTCCTCTAC AGACAGCTAC 
CCTTATAAAC AGGCACACTA TGAGTCTGTG GTCAGTCACT TCTCCTCCGA CTCACAGGGG 
ACTGTCATCT ACAATGTGGA GAACGATGCA GCTTCACAAA GCAGCAGGGA CACAGGGCAC 
CTGACTGACA GCGAGTGCAA TCAGAGGCTG GTTTCCAAAA AGAGCTCACT GGCAGATCGC 
AAGAGGAGCT CTAGCCGGTC TCGTCGAAAA GGAGATGAGG CTCAGTCGTC AGGATACCAC 
AGTGAAGGAG AAACCTTGAA GGAGAAACAA GCCCCCAGAA CTGCCCCCAA ACCACCCAGC 
AGCACCAGCA GGCTGAGGGA ATTTAAAGAG ACAGTGAGCA ATATGATCCA CAGCAGACCA 
CAGCAGATTT CTCAAACTGT CCAGAATTCC CCTCGGGGAG GGAGCAATGT GGATCAGGCA 
GAAACACGAC CCTCAAAAAG CCTCTCTGCT CACTCTCGAG ACTGGGAAGT GGAGAGCACC 
AGTAGCGAGT CCAAGTCCAG TTCCTCCAGC AGGTACCGGC CAACCTGGAG ACCCAAAAGG 
GAATCTCTGA ATATAGACAG CATCTTCAGT AAGGACAAGA GGAAACACTG TGGCTACACT 
CAGCTTAGCC CCTTCACTGA AGAAGCAGGT AAAGAGCTTA ATGAGAACGA GGGGAAGGAA 
CACGCTGGTC AAGAAACGAG ATCAAGCCAG TCAAATGTCA GATACAAGCG TGGAGTGTTA 
GGTCGGGGCA CCCAGCATCA CGTGGTGGAT CAACATCCTC ACCTCATCCA GAGGATGGAG 
TCTGGCTACG AAAGCAGCGA ACGCAACAGC AACAGCCCCG TTAGCCTGGA CATGCCCTTG 
TCTGAAAGCT CAAGCACTCA CAGGGACGTC CATATGAAGC GAACGGGTGG ATTGGTTCCT 
GCCTGGCGTA ACATCCCCAA GTCTCACAGT AGCAGCATCT TGGAAGTGGA GTCTAATTCA 
TCAGCTGGGA GCTGGGCAAA CAGTGCACAC ACCCTTGGAA ACAGTGGGGA GATGTTTGCC 
TCTTCAAAGA GCGAGCTGGA TGAATTGCAA GAGGAGGTGG CAAGGAGAGC ACATGAGCAA 
GAGCTACGCA GAAAAAGAGA AAAGGAAATG GAGGCAGCAA TGGGCTTTAA CCCCCGTCCC 
AGCAGGTTCA TGGATCTCGA TGAACTGCAG AATCAGGCTA AACTAAGACT TGCCATGCAT 
GATGCCAGCG CTAGCACTCA CAGCAGAGCC CTAGTCGATA AGAAGCTGCA AATCAGTATC 
CGAAAAGCCC GGAGCCTCCA GGATCGCATG CAGCACCAAC AACCATCGCA GCCGCTGCCG 
CCGCCAACCT GCCACCCACC CCAGGGTGGC ACTGGGGCCC AGTCTACAAG TGAACAGACT 
GGCCCGCTCC AAGTACTGCT GAGCCAGGAG GTACCACTGG AACCCAACAA AGAAGTGGAA 
TTTGGGTCCA GTTCTTTCTT CCACCCACCT GCTTCCTGCC ATGAATTGCA CTCATCATTA 
TATCCAGAGT CTTCCTCCTT GCGCCCTTCC GAAAGCAATC CATCCAACAG GATCTCTCCA 
AACTTCCAGT CTGCTTCTGC TGATTTTCAT GACCAAGTTC CCTCTTTTCC CTATAGGCAA 
GGGTTGGCAG AGAGGTCTGC GAGAACTGAG AACGATGCCC ACCGTAGTGT GGAATTTGCT 
GACCCCGCAG CCACAGGGCC CAAAGACTTT AGGGAATGCT GTAGTAGCAG CAAGTTAGAA 
GAGGTTCATA ACGTAACGCC TGTTCTCACA CCTCAGTACA AACCCAAGGC TAACACAATA 
AACTCGGGGT CTTTGCCTGC ACTGTTTCCT TGTCCACATC CACCACAAGC AGCATCTCCC 
GAAAAGAATG GCCAGCACAG TCCTTGTTCC AAACTTCCAA GCTCTCCAGT GCGGCCCAGC 
ATTGATCATT TAGGGGCCTA TCATGCAGTG ACCCCTGCAA CTTCATCCCC TACACAACAG 
TGCTACTTGG ATCCTTTGCA GAAAACCAAT AAGTACTCCA GAGCAAACAG CCACGAGGTT 
TTATTACCCT CACAGGATGA ATATGGCACA GCAGAAGGTT GCAAATCTGA GGCTTTTAGT 
CCAAAGGGAC ACGTTCGCTC CTTGGCAGAG CAATTTCAGA AAATGCATGC AGTCTCCCAG 
AGGAAAGGTA CTCATGAAAA AGGCTGGATT GCTGCAGTGT GTCAGGATGA GAAGTCTCCA 
AAGTTAACAC AAAAATCTTT CATCAACCCT TCATCCTCTG GTTACAGACA GCTAATCCAT 
CAAAACCAAG AGACCAAAAC CCAGTCTTCT GAGAAATCAC ACTCAACTGT GGATATTAGG 
GACGGGGTAG TTTCTTTGGA GGGTTTTAGT GCCCAACATG TTGCTTCTAA GAGTATTTGT 
TCTCACAGTG AAGAGCTGTG TAGAAGAGGT GGACAGACTA TAGCTGACCC TGCACCCTAC 
CTGGAAAGGC ACTGTCATGA TGGAGGAATG AAGCAGGTGG ATGATGGAGC TACTGGTAGC 
GTGGTTGCCT CTATGCCTGT GGACCGTTGG GTGGATAATG TTACTAGGTA TTACAGTTCT 
CAGAAGGCTA ATAAGAATAC TGAATGGCTT CCTGTACCTG GGAGGAGTCC ACCCCTTTCT 
GATCAGAGAG CGGTTGGAGA TGACTTGAGC AGGATCCCGG TACATCTGCA CCCACAGTGG 
AATCAAGACA CAGAACAGGA GCTCTCAGAA CTGGAATCCC TCTATCAGAC TAGTCTGCAG 
GCATCTCAGG CCACTAGGCC TTTCTTGGGA AGACAGGACA GTGCTAGGTA TCCATTTGAC 
CAATCAGTCT CTGCGAACCT GAACGTGAGG AAGCTGCATG TTACAGCTGG CATGGGGCTC 
TCCAAAACCC CCACGGCAGA GATCGAGCGC AGTCTGTGTG GATCCGCTGT CTCTTCTGTC 
TCCAAGGTCA CGTACACCAG AGACCATAGC AGGGAACCAG GAGAGGAGGA AATGTACAGT 
GCAGAGAATT TCCGTCGCAT CGCTCGCAGT CTCAGTGGAA CTGTCATCTC AAACAGAGAA 
GAGACCTTGG TCTCTTCTCA CAGTTTTGAA ACACCAAATA TGAGGAAAAT GCCAGTGGAC 
ACCAGCCACC GTTCTTCCAG CTCCACTTCC CTTCCTTTCC CCCATGATCC TCCTGTGTTT 
CCCTTTGATC CCCAGCACAA CCCAACCCAG GTGCAGCACC TACCCCATGA TGTACTGGTG 
CCCAGCATGG TGGGCAAGGC ACGTAAACCC TCAGGAGATC AGAAGAACAT CATCCAGAAA 
CTTTTGGTTG TGCCTGACAG GGGTGAAGCT TTCCAAGGTG GAGACTACCC AGGTGTGGCA 
CTGAGCTACG GGAGTCTCCC TTGTGCACCC AAAGGCACCC TCTCCCAGGG GCAAAAGCCC 
CTCGTTAATC CATATTTAGA AGGTGGAGCA CCAAGTGTCT CTGGCCACCG GCCCAACGCG 
AGCCACGCTG CCAGGCAAGC GGCCACCTTG CCGGAGAAAC GAGCCACGCT CTGGGGGGGA 
CACGTTGGCC AACCTGGCAA GAGTTTACAG GAGGGCTTCC TCCCGCAGCC TTCGCAGCAT 
CCAGTGCATG AATCCTACCC CGTGGGTTGC AGGCTGCCGG CCGGCACAGA CTACAGCACT 
TTAAGAACAC CACGCGAGCC TTCTTGGGAT CCCGGTGCCT CTCAAGGTTG TCCCGTGCCC 
CCTTCTTGCC TGAAGGCCCC GGGTCCAAGG AGAGTTGACA TGCCCCCAGA AGAAGACTGG 
CGGCAGAACA GCTACACCCC TCAGCCTGGC AGAAGGCGGA TGCTGCCCAC GCGTGGGATG 
GAGGGGGCTT TTTCTCCTGC CTCTGAGGCA CACAGAAATA TGCTGGCGAC TGGCACCATG 
CAGAATAACG GGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014811802.1
CDS	845..5962
Translation	MSWKRNYFSVGRGNVQGMFTPRNTTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQITTHKCMGDSCIFCALKSIFNQFQCSSEKVLPSDALRTALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAPHCVSHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPTPDMFGELLQNASTMGDLRNCPSNCGEKIRIRRVLMNSPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVSTQDLPPQVDLQQYSRTCYDSEDSGREPSISSDTRTDSSTDSYPYKQAHYESVVSHFSSDSQGTVIYNVENDAASQSSRDTGHLTDSECNQRLVSKKSSLADRKRSSSRSRRKGDEAQSSGYHSEGETLKEKQAPRTAPKPPSSTSRLREFKETVSNMIHSRPQQISQTVQNSPRGGSNVDQAETRPSKSLSAHSRDWEVESTSSESKSSSSSRYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFTEEAGKELNENEGKEHAGQETRSSQSNVRYKRGVLGRGTQHHVVDQHPHLIQRMESGYESSERNSNSPVSLDMPLSESSSTHRDVHMKRTGGLVPAWRNIPKSHSSSILEVESNSSAGSWANSAHTLGNSGEMFASSKSELDELQEEVARRAHEQELRRKREKEMEAAMGFNPRPSRFMDLDELQNQAKLRLAMHDASASTHSRALVDKKLQISIRKARSLQDRMQHQQPSQPLPPPTCHPPQGGTGAQSTSEQTGPLQVLLSQEVPLEPNKEVEFGSSSFFHPPASCHELHSSLYPESSSLRPSESNPSNRISPNFQSASADFHDQVPSFPYRQGLAERSARTENDAHRSVEFADPAATGPKDFRECCSSSKLEEVHNVTPVLTPQYKPKANTINSGSLPALFPCPHPPQAASPEKNGQHSPCSKLPSSPVRPSIDHLGAYHAVTPATSSPTQQCYLDPLQKTNKYSRANSHEVLLPSQDEYGTAEGCKSEAFSPKGHVRSLAEQFQKMHAVSQRKGTHEKGWIAAVCQDEKSPKLTQKSFINPSSSGYRQLIHQNQETKTQSSEKSHSTVDIRDGVVSLEGFSAQHVASKSICSHSEELCRRGGQTIADPAPYLERHCHDGGMKQVDDGATGSVVASMPVDRWVDNVTRYYSSQKANKNTEWLPVPGRSPPLSDQRAVGDDLSRIPVHLHPQWNQDTEQELSELESLYQTSLQASQATRPFLGRQDSARYPFDQSVSANLNVRKLHVTAGMGLSKTPTAEIERSLCGSAVSSVSKVTYTRDHSREPGEEEMYSAENFRRIARSLSGTVISNREETLVSSHSFETPNMRKMPVDTSHRSSSSTSLPFPHDPPVFPFDPQHNPTQVQHLPHDVLVPSMVGKARKPSGDQKNIIQKLLVVPDRGEAFQGGDYPGVALSYGSLPCAPKGTLSQGQKPLVNPYLEGGAPSVSGHRPNASHAARQAATLPEKRATLWGGHVGQPGKSLQEGFLPQPSQHPVHESYPVGCRLPAGTDYSTLRTPREPSWDPGASQGCPVPPSCLKAPGPRRVDMPPEEDWRQNSYTPQPGRRRMLPTRGMEGAFSPASEAHRNMLATGTMQNNGW

Target ORF information:

RefSeq Version	XM_014956316.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956316.1

ORF Insert Sequence:

ATGTCTTGGA AAAGAAATTA CTTTTCTGTG GGTCGAGGGA ATGTACAGGG CATGTTTACT 
CCACGAAATA CAACCTCAAT AGCACCCAGT AAAGGTCTCA GCAACGAGCC GGGGCAGAAC 
AGTTGCTTCC TGAATAGTGC GCTGCAGGTT CTCTGGCACC TGGACATTTT TCGCCGCAGT 
TTCCGGCAAA TCACAACGCA CAAATGTATG GGTGATTCTT GCATCTTCTG TGCTCTTAAG 
AGTATCTTCA ACCAGTTCCA GTGTAGCAGC GAGAAGGTGC TGCCTTCTGA TGCCCTGCGC 
ACGGCTCTTG CCAAGACGTT TCAGGATGAG CAACGCTTCC AGCTGGGCAT CATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CCTGTTAATG CGAATTCACT TCCACATTGC AGATGAGACA 
AAGGAGGATA TTTGCACTGC ACCACACTGT GTTTCCCATC AGAAGTTTGC TATGACCTTG 
TTTGAGCAGT GTGTTTGTAC CAGCTGTGGT GCCACCTCTG ACCCTTTACC TTTCATCCAG 
ATGGTACATT ATATTTCCAC AACCTCACTC TGCAATCAAG CTATTTGTAT GCTGGAGAGA 
CGAGAGAAAC CCACTCCAGA TATGTTTGGA GAGCTACTAC AGAATGCCAG CACTATGGGA 
GACCTGCGAA ACTGTCCGAG TAACTGCGGG GAAAAGATCC GTATTCGCCG TGTGCTGATG 
AACTCTCCCC AGATCATCAC CATTGGCTTG GTTTGGGACT CGGACCACTC TGATCTGGCT 
GAGGATGTGA TTCACAGTCT GGGCACTTGC CTTAAACTGG GAGATCTCTT CTTCAGAGTA 
ACTGATGACA GAGCAAAGCA CTCAGAGCTG TATTTGGTGG GGATGATCTG TTACTATGGA 
AAACACTATT CTACCTTCTT CTTCCAAACT AAAATCCGCA AGTGGATGTA CTTTGATGAT 
GCTCATGTCA AAGAGATCGG TCCAAAGTGG AAAGATGTGG TGACGAAGTG CATTAAGGGT 
CACTACCAGC CTTTGCTGCT GCTGTACGCA GATCCCAGAG GAACTCCAGT GTCAACACAA 
GACTTGCCCC CTCAAGTGGA TTTACAGCAA TACAGCAGGA CTTGCTATGA TAGCGAAGAC 
TCGGGGAGAG AGCCTTCCAT CTCGAGTGAC ACCAGGACAG ACTCCTCTAC AGACAGCTAC 
CCTTATAAAC AGGCACACTA TGAGTCTGTG GTCAGTCACT TCTCCTCCGA CTCACAGGGG 
ACTGTCATCT ACAATGTGGA GAACGATGCA GCTTCACAAA GCAGCAGGGA CACAGGGCAC 
CTGACTGACA GCGAGTGCAA TCAGAGGCTG GTTTCCAAAA AGAGCTCACT GGCAGATCGC 
AAGAGGAGCT CTAGCCGGTC TCGTCGAAAA GGAGATGAGG CTCAGTCGTC AGGATACCAC 
AGTGAAGGAG AAACCTTGAA GGAGAAACAA GCCCCCAGAA CTGCCCCCAA ACCACCCAGC 
AGCACCAGCA GGCTGAGGGA ATTTAAAGAG ACAGTGAGCA ATATGATCCA CAGCAGACCA 
CAGCAGATTT CTCAAACTGT CCAGAATTCC CCTCGGGGAG GGAGCAATGT GGATCAGGCA 
GAAACACGAC CCTCAAAAAG CCTCTCTGCT CACTCTCGAG ACTGGGAAGT GGAGAGCACC 
AGTAGCGAGT CCAAGTCCAG TTCCTCCAGC AGGTACCGGC CAACCTGGAG ACCCAAAAGG 
GAATCTCTGA ATATAGACAG CATCTTCAGT AAGGACAAGA GGAAACACTG TGGCTACACT 
CAGCTTAGCC CCTTCACTGA AGAAGCAGGT AAAGAGCTTA ATGAGAACGA GGGGAAGGAA 
CACGCTGGTC AAGAAACGAG ATCAAGCCAG TCAAATGTCA GATACAAGCG TGGAGTGTTA 
GGTCGGGGCA CCCAGCATCA CGTGGTGGAT CAACATCCTC ACCTCATCCA GAGGATGGAG 
TCTGGCTACG AAAGCAGCGA ACGCAACAGC AACAGCCCCG TTAGCCTGGA CATGCCCTTG 
TCTGAAAGCT CAAGCACTCA CAGGGACGTC CATATGAAGC GAACGGGTGG ATTGGTTCCT 
GCCTGGCGTA ACATCCCCAA GTCTCACAGT AGCAGCATCT TGGAAGTGGA GTCTAATTCA 
TCAGCTGGGA GCTGGGCAAA CAGTGCACAC ACCCTTGGAA ACAGTGGGGA GATGTTTGCC 
TCTTCAAAGA GCGAGCTGGA TGAATTGCAA GAGGAGGTGG CAAGGAGAGC ACATGAGCAA 
GAGCTACGCA GAAAAAGAGA AAAGGAAATG GAGGCAGCAA TGGGCTTTAA CCCCCGTCCC 
AGCAGGTTCA TGGATCTCGA TGAACTGCAG AATCAGGCTA AACTAAGACT TGCCATGCAT 
GATGCCAGCG CTAGCACTCA CAGCAGAGCC CTAGTCGATA AGAAGCTGCA AATCAGTATC 
CGAAAAGCCC GGAGCCTCCA GGATCGCATG CAGCACCAAC AACCATCGCA GCCGCTGCCG 
CCGCCAACCT GCCACCCACC CCAGGGTGGC ACTGGGGCCC AGTCTACAAG TGAACAGACT 
GGCCCGCTCC AAGTACTGCT GAGCCAGGAG GTACCACTGG AACCCAACAA AGAAGTGGAA 
TTTGGGTCCA GTTCTTTCTT CCACCCACCT GCTTCCTGCC ATGAATTGCA CTCATCATTA 
TATCCAGAGT CTTCCTCCTT GCGCCCTTCC GAAAGCAATC CATCCAACAG GATCTCTCCA 
AACTTCCAGT CTGCTTCTGC TGATTTTCAT GACCAAGTTC CCTCTTTTCC CTATAGGCAA 
GGGTTGGCAG AGAGGTCTGC GAGAACTGAG AACGATGCCC ACCGTAGTGT GGAATTTGCT 
GACCCCGCAG CCACAGGGCC CAAAGACTTT AGGGAATGCT GTAGTAGCAG CAAGTTAGAA 
GAGGTTCATA ACGTAACGCC TGTTCTCACA CCTCAGTACA AACCCAAGGC TAACACAATA 
AACTCGGGGT CTTTGCCTGC ACTGTTTCCT TGTCCACATC CACCACAAGC AGCATCTCCC 
GAAAAGAATG GCCAGCACAG TCCTTGTTCC AAACTTCCAA GCTCTCCAGT GCGGCCCAGC 
ATTGATCATT TAGGGGCCTA TCATGCAGTG ACCCCTGCAA CTTCATCCCC TACACAACAG 
TGCTACTTGG ATCCTTTGCA GAAAACCAAT AAGTACTCCA GAGCAAACAG CCACGAGGTT 
TTATTACCCT CACAGGATGA ATATGGCACA GCAGAAGGTT GCAAATCTGA GGCTTTTAGT 
CCAAAGGGAC ACGTTCGCTC CTTGGCAGAG CAATTTCAGA AAATGCATGC AGTCTCCCAG 
AGGAAAGGTA CTCATGAAAA AGGCTGGATT GCTGCAGTGT GTCAGGATGA GAAGTCTCCA 
AAGTTAACAC AAAAATCTTT CATCAACCCT TCATCCTCTG GTTACAGACA GCTAATCCAT 
CAAAACCAAG AGACCAAAAC CCAGTCTTCT GAGAAATCAC ACTCAACTGT GGATATTAGG 
GACGGGGTAG TTTCTTTGGA GGGTTTTAGT GCCCAACATG TTGCTTCTAA GAGTATTTGT 
TCTCACAGTG AAGAGCTGTG TAGAAGAGGT GGACAGACTA TAGCTGACCC TGCACCCTAC 
CTGGAAAGGC ACTGTCATGA TGGAGGAATG AAGCAGGTGG ATGATGGAGC TACTGGTAGC 
GTGGTTGCCT CTATGCCTGT GGACCGTTGG GTGGATAATG TTACTAGGTA TTACAGTTCT 
CAGAAGGCTA ATAAGAATAC TGAATGGCTT CCTGTACCTG GGAGGAGTCC ACCCCTTTCT 
GATCAGAGAG CGGTTGGAGA TGACTTGAGC AGGATCCCGG TACATCTGCA CCCACAGTGG 
AATCAAGACA CAGAACAGGA GCTCTCAGAA CTGGAATCCC TCTATCAGAC TAGTCTGCAG 
GCATCTCAGG CCACTAGGCC TTTCTTGGGA AGACAGGACA GTGCTAGGTA TCCATTTGAC 
CAATCAGTCT CTGCGAACCT GAACGTGAGG AAGCTGCATG TTACAGCTGG CATGGGGCTC 
TCCAAAACCC CCACGGCAGA GATCGAGCGC AGTCTGTGTG GATCCGCTGT CTCTTCTGTC 
TCCAAGGTCA CGTACACCAG AGACCATAGC AGGGAACCAG GAGAGGAGGA AATGTACAGT 
GCAGAGAATT TCCGTCGCAT CGCTCGCAGT CTCAGTGGAA CTGTCATCTC AAACAGAGAA 
GAGACCTTGG TCTCTTCTCA CAGTTTTGAA ACACCAAATA TGAGGAAAAT GCCAGTGGAC 
ACCAGCCACC GTTCTTCCAG CTCCACTTCC CTTCCTTTCC CCCATGATCC TCCTGTGTTT 
CCCTTTGATC CCCAGCACAA CCCAACCCAG GTGCAGCACC TACCCCATGA TGTACTGGTG 
CCCAGCATGG TGGGCAAGGC ACGTAAACCC TCAGGAGATC AGAAGAACAT CATCCAGAAA 
CTTTTGGTTG TGCCTGACAG GGGTGAAGCT TTCCAAGGTG GAGACTACCC AGGTGTGGCA 
CTGAGCTACG GGAGTCTCCC TTGTGCACCC AAAGGCACCC TCTCCCAGGG GCAAAAGCCC 
CTCGTTAATC CATATTTAGA AGGTGGAGCA CCAAGTGTCT CTGGCCACCG GCCCAACGCG 
AGCCACGCTG CCAGGCAAGC GGCCACCTTG CCGGAGAAAC GAGCCACGCT CTGGGGGGGA 
CACGTTGGCC AACCTGGCAA GAGTTTACAG GAGGGCTTCC TCCCGCAGCC TTCGCAGCAT 
CCAGTGCATG AATCCTACCC CGTGGGTTGC AGGCTGCCGG CCGGCACAGA CTACAGCACT 
TTAAGAACAC CACGCGAGCC TTCTTGGGAT CCCGGTGCCT CTCAAGGTTG TCCCGTGCCC 
CCTTCTTGCC TGAAGGCCCC GGGTCCAAGG AGAGTTGACA TGCCCCCAGA AGAAGACTGG 
CGGCAGAACA GCTACACCCC TCAGCCTGGC AGAAGGCGGA TGCTGCCCAC GCGTGGGATG 
GAGGGGGCTT TTTCTCCTGC CTCTGAGGCA CACAGAAATA TGCTGGCGAC TGGCACCATG 
CAGAATAACG GGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP54 ( XM_014956317.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811803.1 Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.

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CloneID	OCa75523
Clone ID Related Accession (Same CDS sequence)	XM_014956317.1
Accession Version	XM_014956317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	inactive ubiquitin carboxyl-terminal hydrolase 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTATG AGACAAAGGA GGATATTTGC ACTGCACCAC ACTGTGTTTC CCATCAGAAG 
TTTGCTATGA CCTTGTTTGA GCAGTGTGTT TGTACCAGCT GTGGTGCCAC CTCTGACCCT 
TTACCTTTCA TCCAGATGGT ACATTATATT TCCACAACCT CACTCTGCAA TCAAGCTATT 
TGTATGCTGG AGAGACGAGA GAAACCCACT CCAGATATGT TTGGAGAGCT ACTACAGAAT 
GCCAGCACTA TGGGAGACCT GCGAAACTGT CCGAGTAACT GCGGGGAAAA GATCCGTATT 
CGCCGTGTGC TGATGAACTC TCCCCAGATC ATCACCATTG GCTTGGTTTG GGACTCGGAC 
CACTCTGATC TGGCTGAGGA TGTGATTCAC AGTCTGGGCA CTTGCCTTAA ACTGGGAGAT 
CTCTTCTTCA GAGTAACTGA TGACAGAGCA AAGCACTCAG AGCTGTATTT GGTGGGGATG 
ATCTGTTACT ATGGAAAACA CTATTCTACC TTCTTCTTCC AAACTAAAAT CCGCAAGTGG 
ATGTACTTTG ATGATGCTCA TGTCAAAGAG ATCGGTCCAA AGTGGAAAGA TGTGGTGACG 
AAGTGCATTA AGGGTCACTA CCAGCCTTTG CTGCTGCTGT ACGCAGATCC CAGAGGAACT 
CCAGTGTCAA CACAAGACTT GCCCCCTCAA GTGGATTTAC AGCAATACAG CAGGACTTGC 
TATGATAGCG AAGACTCGGG GAGAGAGCCT TCCATCTCGA GTGACACCAG GACAGACTCC 
TCTACAGACA GCTACCCTTA TAAACAGGCA CACTATGAGT CTGTGGTCAG TCACTTCTCC 
TCCGACTCAC AGGGGACTGT CATCTACAAT GTGGAGAACG ATGCAGCTTC ACAAAGCAGC 
AGGGACACAG GGCACCTGAC TGACAGCGAG TGCAATCAGA GGCTGGTTTC CAAAAAGAGC 
TCACTGGCAG ATCGCAAGAG GAGCTCTAGC CGGTCTCGTC GAAAAGGAGA TGAGGCTCAG 
TCGTCAGGAT ACCACAGTGA AGGAGAAACC TTGAAGGAGA AACAAGCCCC CAGAACTGCC 
CCCAAACCAC CCAGCAGCAC CAGCAGGCTG AGGGAATTTA AAGAGACAGT GAGCAATATG 
ATCCACAGCA GACCACAGCA GATTTCTCAA ACTGTCCAGA ATTCCCCTCG GGGAGGGAGC 
AATGTGGATC AGGCAGAAAC ACGACCCTCA AAAAGCCTCT CTGCTCACTC TCGAGACTGG 
GAAGTGGAGA GCACCAGTAG CGAGTCCAAG TCCAGTTCCT CCAGCAGGTA CCGGCCAACC 
TGGAGACCCA AAAGGGAATC TCTGAATATA GACAGCATCT TCAGTAAGGA CAAGAGGAAA 
CACTGTGGCT ACACTCAGCT TAGCCCCTTC ACTGAAGAAG CAGGTAAAGA GCTTAATGAG 
AACGAGGGGA AGGAACACGC TGGTCAAGAA ACGAGATCAA GCCAGTCAAA TGTCAGATAC 
AAGCGTGGAG TGTTAGGTCG GGGCACCCAG CATCACGTGG TGGATCAACA TCCTCACCTC 
ATCCAGAGGA TGGAGTCTGG CTACGAAAGC AGCGAACGCA ACAGCAACAG CCCCGTTAGC 
CTGGACATGC CCTTGTCTGA AAGCTCAAGC ACTCACAGGG ACGTCCATAT GAAGCGAACG 
GGTGGATTGG TTCCTGCCTG GCGTAACATC CCCAAGTCTC ACAGTAGCAG CATCTTGGAA 
GTGGAGTCTA ATTCATCAGC TGGGAGCTGG GCAAACAGTG CACACACCCT TGGAAACAGT 
GGGGAGATGT TTGCCTCTTC AAAGAGCGAG CTGGATGAAT TGCAAGAGGA GGTGGCAAGG 
AGAGCACATG AGCAAGAGCT ACGCAGAAAA AGAGAAAAGG AAATGGAGGC AGCAATGGGC 
TTTAACCCCC GTCCCAGCAG GTTCATGGAT CTCGATGAAC TGCAGAATCA GGGGAGGAGT 
GATGGCTTTG AGAAGTCGAT GCAGGAGGCA GACTCAATCT TTGAAGAGTC GCTGAATCAG 
GAGCAGAAGG GGGATTGTGC TGCAGCTTTG GCTCTCTGTA ACGAAGCTAT ATCTAAACTA 
AGACTTGCCA TGCATGATGC CAGCGCTAGC ACTCACAGCA GAGCCCTAGT CGATAAGAAG 
CTGCAAATCA GTATCCGAAA AGCCCGGAGC CTCCAGGATC GCATGCAGCA CCAACAACCA 
TCGCAGCCGC TGCCGCCGCC AACCTGCCAC CCACCCCAGG GTGGCACTGG GGCCCAGTCT 
ACAAGTGAAC AGACTGGCCC GCTCCAAGTA CTGCTGAGCC AGGAGGTACC ACTGGAACCC 
AACAAAGAAG TGGAATTTGG GTCCAGTTCT TTCTTCCACC CACCTGCTTC CTGCCATGAA 
TTGCACTCAT CATTATATCC AGAGTCTTCC TCCTTGCGCC CTTCCGAAAG CAATCCATCC 
AACAGGATCT CTCCAAACTT CCAGTCTGCT TCTGCTGATT TTCATGACCA AGTTCCCTCT 
TTTCCCTATA GGCAAGGGTT GGCAGAGAGG TCTGCGAGAA CTGAGAACGA TGCCCACCGT 
AGTGTGGAAT TTGCTGACCC CGCAGCCACA GGGCCCAAAG ACTTTAGGGA ATGCTGTAGT 
AGCAGCAAGT TAGAAGAGGT TCATAACGTA ACGCCTGTTC TCACACCTCA GTACAAACCC 
AAGGCTAACA CAATAAACTC GGGGTCTTTG CCTGCACTGT TTCCTTGTCC ACATCCACCA 
CAAGCAGCAT CTCCCGAAAA GAATGGCCAG CACAGTCCTT GTTCCAAACT TCCAAGCTCT 
CCAGTGCGGC CCAGCATTGA TCATTTAGGG GCCTATCATG CAGTGACCCC TGCAACTTCA 
TCCCCTACAC AACAGTGCTA CTTGGATCCT TTGCAGAAAA CCAATAAGTA CTCCAGAGCA 
AACAGCCACG AGGTTTTATT ACCCTCACAG GATGAATATG GCACAGCAGA AGGTTGCAAA 
TCTGAGGCTT TTAGTCCAAA GGGACACGTT CGCTCCTTGG CAGAGCAATT TCAGAAAATG 
CATGCAGTCT CCCAGAGGAA AGGTACTCAT GAAAAAGGCT GGATTGCTGC AGTGTGTCAG 
GATGAGAAGT CTCCAAAGTT AACACAAAAA TCTTTCATCA ACCCTTCATC CTCTGGTTAC 
AGACAGCTAA TCCATCAAAA CCAAGAGACC AAAACCCAGT CTTCTGAGAA ATCACACTCA 
ACTGTGGATA TTAGGGACGG GGTAGTTTCT TTGGAGGGTT TTAGTGCCCA ACATGTTGCT 
TCTAAGAGTA TTTGTTCTCA CAGTGAAGAG CTGTGTAGAA GAGGTGGACA GACTATAGCT 
GACCCTGCAC CCTACCTGGA AAGGCACTGT CATGATGGAG GAATGAAGCA GGTGGATGAT 
GGAGCTACTG GTAGCGTGGT TGCCTCTATG CCTGTGGACC GTTGGGTGGA TAATGTTACT 
AGGTATTACA GTTCTCAGAA GGCTAATAAG AATACTGAAT GGCTTCCTGT ACCTGGGAGG 
AGTCCACCCC TTTCTGATCA GAGAGCGGTT GGAGATGACT TGAGCAGGAT CCCGGTACAT 
CTGCACCCAC AGTGGAATCA AGACACAGAA CAGGAGCTCT CAGAACTGGA ATCCCTCTAT 
CAGACTAGTC TGCAGGCATC TCAGGCCACT AGGCCTTTCT TGGGAAGACA GGACAGTGCT 
AGGTATCCAT TTGACCAATC AGTCTCTGCG AACCTGAACG TGAGGAAGCT GCATGTTACA 
GCTGGCATGG GGCTCTCCAA AACCCCCACG GCAGAGATCG AGCGCAGTCT GTGTGGATCC 
GCTGTCTCTT CTGTCTCCAA GGTCACGTAC ACCAGAGACC ATAGCAGGGA ACCAGGAGAG 
GAGGAAATGT ACAGTGCAGA GAATTTCCGT CGCATCGCTC GCAGTCTCAG TGGAACTGTC 
ATCTCAAACA GAGAAGAGAC CTTGGTCTCT TCTCACAGTT TTGAAACACC AAATATGAGG 
AAAATGCCAG TGGACACCAG CCACCGTTCT TCCAGCTCCA CTTCCCTTCC TTTCCCCCAT 
GATCCTCCTG TGTTTCCCTT TGATCCCCAG CACAACCCAA CCCAGGTGCA GCACCTACCC 
CATGATGTAC TGGTGCCCAG CATGGTGGGC AAGGCACGTA AACCCTCAGG AGATCAGAAG 
AACATCATCC AGAAACTTTT GGTTGTGCCT GACAGGGGTG AAGCTTTCCA AGGTGGAGAC 
TACCCAGGTG TGGCACTGAG CTACGGGAGT CTCCCTTGTG CACCCAAAGG CACCCTCTCC 
CAGGGGCAAA AGCCCCTCGT TAATCCATAT TTAGAAGGTG GAGCACCAAG TGTCTCTGGC 
CACCGGCCCA ACGCGAGCCA CGCTGCCAGG CAAGCGGCCA CCTTGCCGGA GAAACGAGCC 
ACGCTCTGGG GGGGACACGT TGGCCAACCT GGCAAGAGTT TACAGGAGGG CTTCCTCCCG 
CAGCCTTCGC AGCATCCAGT GCATGAATCC TACCCCGTGG GTTGCAGGCT GCCGGCCGGC 
ACAGACTACA GCACTTTAAG AACACCACGC GAGCCTTCTT GGGATCCCGG TGCCTCTCAA 
GGTTGTCCCG TGCCCCCTTC TTGCCTGAAG GCCCCGGGTC CAAGGAGAGT TGACATGCCC 
CCAGAAGAAG ACTGGCGGCA GAACAGCTAC ACCCCTCAGC CTGGCAGAAG GCGGATGCTG 
CCCACGCGTG GGATGGAGGG GGCTTTTTCT CCTGCCTCTG AGGCACACAG AAATATGCTG 
GCGACTGGCA CCATGCAGAA TAACGGGTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014811803.1
CDS	77..4909
Translation	MFYETKEDICTAPHCVSHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPTPDMFGELLQNASTMGDLRNCPSNCGEKIRIRRVLMNSPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKHSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPRGTPVSTQDLPPQVDLQQYSRTCYDSEDSGREPSISSDTRTDSSTDSYPYKQAHYESVVSHFSSDSQGTVIYNVENDAASQSSRDTGHLTDSECNQRLVSKKSSLADRKRSSSRSRRKGDEAQSSGYHSEGETLKEKQAPRTAPKPPSSTSRLREFKETVSNMIHSRPQQISQTVQNSPRGGSNVDQAETRPSKSLSAHSRDWEVESTSSESKSSSSSRYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFTEEAGKELNENEGKEHAGQETRSSQSNVRYKRGVLGRGTQHHVVDQHPHLIQRMESGYESSERNSNSPVSLDMPLSESSSTHRDVHMKRTGGLVPAWRNIPKSHSSSILEVESNSSAGSWANSAHTLGNSGEMFASSKSELDELQEEVARRAHEQELRRKREKEMEAAMGFNPRPSRFMDLDELQNQGRSDGFEKSMQEADSIFEESLNQEQKGDCAAALALCNEAISKLRLAMHDASASTHSRALVDKKLQISIRKARSLQDRMQHQQPSQPLPPPTCHPPQGGTGAQSTSEQTGPLQVLLSQEVPLEPNKEVEFGSSSFFHPPASCHELHSSLYPESSSLRPSESNPSNRISPNFQSASADFHDQVPSFPYRQGLAERSARTENDAHRSVEFADPAATGPKDFRECCSSSKLEEVHNVTPVLTPQYKPKANTINSGSLPALFPCPHPPQAASPEKNGQHSPCSKLPSSPVRPSIDHLGAYHAVTPATSSPTQQCYLDPLQKTNKYSRANSHEVLLPSQDEYGTAEGCKSEAFSPKGHVRSLAEQFQKMHAVSQRKGTHEKGWIAAVCQDEKSPKLTQKSFINPSSSGYRQLIHQNQETKTQSSEKSHSTVDIRDGVVSLEGFSAQHVASKSICSHSEELCRRGGQTIADPAPYLERHCHDGGMKQVDDGATGSVVASMPVDRWVDNVTRYYSSQKANKNTEWLPVPGRSPPLSDQRAVGDDLSRIPVHLHPQWNQDTEQELSELESLYQTSLQASQATRPFLGRQDSARYPFDQSVSANLNVRKLHVTAGMGLSKTPTAEIERSLCGSAVSSVSKVTYTRDHSREPGEEEMYSAENFRRIARSLSGTVISNREETLVSSHSFETPNMRKMPVDTSHRSSSSTSLPFPHDPPVFPFDPQHNPTQVQHLPHDVLVPSMVGKARKPSGDQKNIIQKLLVVPDRGEAFQGGDYPGVALSYGSLPCAPKGTLSQGQKPLVNPYLEGGAPSVSGHRPNASHAARQAATLPEKRATLWGGHVGQPGKSLQEGFLPQPSQHPVHESYPVGCRLPAGTDYSTLRTPREPSWDPGASQGCPVPPSCLKAPGPRRVDMPPEEDWRQNSYTPQPGRRRMLPTRGMEGAFSPASEAHRNMLATGTMQNNGW

Target ORF information:

RefSeq Version	XM_014956317.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax ubiquitin specific peptidase 54 (USP54), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956317.1

ORF Insert Sequence:

ATGTTTTATG AGACAAAGGA GGATATTTGC ACTGCACCAC ACTGTGTTTC CCATCAGAAG 
TTTGCTATGA CCTTGTTTGA GCAGTGTGTT TGTACCAGCT GTGGTGCCAC CTCTGACCCT 
TTACCTTTCA TCCAGATGGT ACATTATATT TCCACAACCT CACTCTGCAA TCAAGCTATT 
TGTATGCTGG AGAGACGAGA GAAACCCACT CCAGATATGT TTGGAGAGCT ACTACAGAAT 
GCCAGCACTA TGGGAGACCT GCGAAACTGT CCGAGTAACT GCGGGGAAAA GATCCGTATT 
CGCCGTGTGC TGATGAACTC TCCCCAGATC ATCACCATTG GCTTGGTTTG GGACTCGGAC 
CACTCTGATC TGGCTGAGGA TGTGATTCAC AGTCTGGGCA CTTGCCTTAA ACTGGGAGAT 
CTCTTCTTCA GAGTAACTGA TGACAGAGCA AAGCACTCAG AGCTGTATTT GGTGGGGATG 
ATCTGTTACT ATGGAAAACA CTATTCTACC TTCTTCTTCC AAACTAAAAT CCGCAAGTGG 
ATGTACTTTG ATGATGCTCA TGTCAAAGAG ATCGGTCCAA AGTGGAAAGA TGTGGTGACG 
AAGTGCATTA AGGGTCACTA CCAGCCTTTG CTGCTGCTGT ACGCAGATCC CAGAGGAACT 
CCAGTGTCAA CACAAGACTT GCCCCCTCAA GTGGATTTAC AGCAATACAG CAGGACTTGC 
TATGATAGCG AAGACTCGGG GAGAGAGCCT TCCATCTCGA GTGACACCAG GACAGACTCC 
TCTACAGACA GCTACCCTTA TAAACAGGCA CACTATGAGT CTGTGGTCAG TCACTTCTCC 
TCCGACTCAC AGGGGACTGT CATCTACAAT GTGGAGAACG ATGCAGCTTC ACAAAGCAGC 
AGGGACACAG GGCACCTGAC TGACAGCGAG TGCAATCAGA GGCTGGTTTC CAAAAAGAGC 
TCACTGGCAG ATCGCAAGAG GAGCTCTAGC CGGTCTCGTC GAAAAGGAGA TGAGGCTCAG 
TCGTCAGGAT ACCACAGTGA AGGAGAAACC TTGAAGGAGA AACAAGCCCC CAGAACTGCC 
CCCAAACCAC CCAGCAGCAC CAGCAGGCTG AGGGAATTTA AAGAGACAGT GAGCAATATG 
ATCCACAGCA GACCACAGCA GATTTCTCAA ACTGTCCAGA ATTCCCCTCG GGGAGGGAGC 
AATGTGGATC AGGCAGAAAC ACGACCCTCA AAAAGCCTCT CTGCTCACTC TCGAGACTGG 
GAAGTGGAGA GCACCAGTAG CGAGTCCAAG TCCAGTTCCT CCAGCAGGTA CCGGCCAACC 
TGGAGACCCA AAAGGGAATC TCTGAATATA GACAGCATCT TCAGTAAGGA CAAGAGGAAA 
CACTGTGGCT ACACTCAGCT TAGCCCCTTC ACTGAAGAAG CAGGTAAAGA GCTTAATGAG 
AACGAGGGGA AGGAACACGC TGGTCAAGAA ACGAGATCAA GCCAGTCAAA TGTCAGATAC 
AAGCGTGGAG TGTTAGGTCG GGGCACCCAG CATCACGTGG TGGATCAACA TCCTCACCTC 
ATCCAGAGGA TGGAGTCTGG CTACGAAAGC AGCGAACGCA ACAGCAACAG CCCCGTTAGC 
CTGGACATGC CCTTGTCTGA AAGCTCAAGC ACTCACAGGG ACGTCCATAT GAAGCGAACG 
GGTGGATTGG TTCCTGCCTG GCGTAACATC CCCAAGTCTC ACAGTAGCAG CATCTTGGAA 
GTGGAGTCTA ATTCATCAGC TGGGAGCTGG GCAAACAGTG CACACACCCT TGGAAACAGT 
GGGGAGATGT TTGCCTCTTC AAAGAGCGAG CTGGATGAAT TGCAAGAGGA GGTGGCAAGG 
AGAGCACATG AGCAAGAGCT ACGCAGAAAA AGAGAAAAGG AAATGGAGGC AGCAATGGGC 
TTTAACCCCC GTCCCAGCAG GTTCATGGAT CTCGATGAAC TGCAGAATCA GGGGAGGAGT 
GATGGCTTTG AGAAGTCGAT GCAGGAGGCA GACTCAATCT TTGAAGAGTC GCTGAATCAG 
GAGCAGAAGG GGGATTGTGC TGCAGCTTTG GCTCTCTGTA ACGAAGCTAT ATCTAAACTA 
AGACTTGCCA TGCATGATGC CAGCGCTAGC ACTCACAGCA GAGCCCTAGT CGATAAGAAG 
CTGCAAATCA GTATCCGAAA AGCCCGGAGC CTCCAGGATC GCATGCAGCA CCAACAACCA 
TCGCAGCCGC TGCCGCCGCC AACCTGCCAC CCACCCCAGG GTGGCACTGG GGCCCAGTCT 
ACAAGTGAAC AGACTGGCCC GCTCCAAGTA CTGCTGAGCC AGGAGGTACC ACTGGAACCC 
AACAAAGAAG TGGAATTTGG GTCCAGTTCT TTCTTCCACC CACCTGCTTC CTGCCATGAA 
TTGCACTCAT CATTATATCC AGAGTCTTCC TCCTTGCGCC CTTCCGAAAG CAATCCATCC 
AACAGGATCT CTCCAAACTT CCAGTCTGCT TCTGCTGATT TTCATGACCA AGTTCCCTCT 
TTTCCCTATA GGCAAGGGTT GGCAGAGAGG TCTGCGAGAA CTGAGAACGA TGCCCACCGT 
AGTGTGGAAT TTGCTGACCC CGCAGCCACA GGGCCCAAAG ACTTTAGGGA ATGCTGTAGT 
AGCAGCAAGT TAGAAGAGGT TCATAACGTA ACGCCTGTTC TCACACCTCA GTACAAACCC 
AAGGCTAACA CAATAAACTC GGGGTCTTTG CCTGCACTGT TTCCTTGTCC ACATCCACCA 
CAAGCAGCAT CTCCCGAAAA GAATGGCCAG CACAGTCCTT GTTCCAAACT TCCAAGCTCT 
CCAGTGCGGC CCAGCATTGA TCATTTAGGG GCCTATCATG CAGTGACCCC TGCAACTTCA 
TCCCCTACAC AACAGTGCTA CTTGGATCCT TTGCAGAAAA CCAATAAGTA CTCCAGAGCA 
AACAGCCACG AGGTTTTATT ACCCTCACAG GATGAATATG GCACAGCAGA AGGTTGCAAA 
TCTGAGGCTT TTAGTCCAAA GGGACACGTT CGCTCCTTGG CAGAGCAATT TCAGAAAATG 
CATGCAGTCT CCCAGAGGAA AGGTACTCAT GAAAAAGGCT GGATTGCTGC AGTGTGTCAG 
GATGAGAAGT CTCCAAAGTT AACACAAAAA TCTTTCATCA ACCCTTCATC CTCTGGTTAC 
AGACAGCTAA TCCATCAAAA CCAAGAGACC AAAACCCAGT CTTCTGAGAA ATCACACTCA 
ACTGTGGATA TTAGGGACGG GGTAGTTTCT TTGGAGGGTT TTAGTGCCCA ACATGTTGCT 
TCTAAGAGTA TTTGTTCTCA CAGTGAAGAG CTGTGTAGAA GAGGTGGACA GACTATAGCT 
GACCCTGCAC CCTACCTGGA AAGGCACTGT CATGATGGAG GAATGAAGCA GGTGGATGAT 
GGAGCTACTG GTAGCGTGGT TGCCTCTATG CCTGTGGACC GTTGGGTGGA TAATGTTACT 
AGGTATTACA GTTCTCAGAA GGCTAATAAG AATACTGAAT GGCTTCCTGT ACCTGGGAGG 
AGTCCACCCC TTTCTGATCA GAGAGCGGTT GGAGATGACT TGAGCAGGAT CCCGGTACAT 
CTGCACCCAC AGTGGAATCA AGACACAGAA CAGGAGCTCT CAGAACTGGA ATCCCTCTAT 
CAGACTAGTC TGCAGGCATC TCAGGCCACT AGGCCTTTCT TGGGAAGACA GGACAGTGCT 
AGGTATCCAT TTGACCAATC AGTCTCTGCG AACCTGAACG TGAGGAAGCT GCATGTTACA 
GCTGGCATGG GGCTCTCCAA AACCCCCACG GCAGAGATCG AGCGCAGTCT GTGTGGATCC 
GCTGTCTCTT CTGTCTCCAA GGTCACGTAC ACCAGAGACC ATAGCAGGGA ACCAGGAGAG 
GAGGAAATGT ACAGTGCAGA GAATTTCCGT CGCATCGCTC GCAGTCTCAG TGGAACTGTC 
ATCTCAAACA GAGAAGAGAC CTTGGTCTCT TCTCACAGTT TTGAAACACC AAATATGAGG 
AAAATGCCAG TGGACACCAG CCACCGTTCT TCCAGCTCCA CTTCCCTTCC TTTCCCCCAT 
GATCCTCCTG TGTTTCCCTT TGATCCCCAG CACAACCCAA CCCAGGTGCA GCACCTACCC 
CATGATGTAC TGGTGCCCAG CATGGTGGGC AAGGCACGTA AACCCTCAGG AGATCAGAAG 
AACATCATCC AGAAACTTTT GGTTGTGCCT GACAGGGGTG AAGCTTTCCA AGGTGGAGAC 
TACCCAGGTG TGGCACTGAG CTACGGGAGT CTCCCTTGTG CACCCAAAGG CACCCTCTCC 
CAGGGGCAAA AGCCCCTCGT TAATCCATAT TTAGAAGGTG GAGCACCAAG TGTCTCTGGC 
CACCGGCCCA ACGCGAGCCA CGCTGCCAGG CAAGCGGCCA CCTTGCCGGA GAAACGAGCC 
ACGCTCTGGG GGGGACACGT TGGCCAACCT GGCAAGAGTT TACAGGAGGG CTTCCTCCCG 
CAGCCTTCGC AGCATCCAGT GCATGAATCC TACCCCGTGG GTTGCAGGCT GCCGGCCGGC 
ACAGACTACA GCACTTTAAG AACACCACGC GAGCCTTCTT GGGATCCCGG TGCCTCTCAA 
GGTTGTCCCG TGCCCCCTTC TTGCCTGAAG GCCCCGGGTC CAAGGAGAGT TGACATGCCC 
CCAGAAGAAG ACTGGCGGCA GAACAGCTAC ACCCCTCAGC CTGGCAGAAG GCGGATGCTG 
CCCACGCGTG GGATGGAGGG GGCTTTTTCT CCTGCCTCTG AGGCACACAG AAATATGCTG 
GCGACTGGCA CCATGCAGAA TAACGGGTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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USP54 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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