LOC106896755 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106896755
Entrez Gene ID	106896755
Full Name	myosin heavy chain, skeletal muscle, adult-like
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014956971.1	XP_014812457.1	myosin heavy chain, skeletal muscle, adult-like

Pathways from BioSystems
Gene Ontology

The following LOC106896755 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106896755 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa76009	XM_014956971.1 Latest version!	Calidris pugnax myosin heavy chain, skeletal muscle, adult-like (LOC106896755), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106896755 ( XM_014956971.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014812457.1 Calidris pugnax myosin heavy chain, skeletal muscle, adult-like (LOC106896755), mRNA.

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CloneID	OCa76009
Clone ID Related Accession (Same CDS sequence)	XM_014956971.1
Accession Version	XM_014956971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	myosin heavy chain, skeletal muscle, adult-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCTTCAG ATGCTGAGAT GGCCATCTTT GGGGAGGCAG CTGTGTATCT CCGAAAGTCA 
GAAAAGGAGA GAATCGAGGC CCAGAACAAG CCTTTTGATG CCAAGTCATC TGTCTTTGTG 
GCACATCCTA AGGAATCTTT TGTGAAAGGG ACAATCCAGA GCAAAGAATC AGGGAAAGTC 
ACTGTCAAGA CAGAAGGTGG AGAGACCCTG ACCGTGAAGG AAGATCAAAT CTTCTCCATG 
AACCCTCCCA AGTATGATAA AATCGAGGAC ATGGCCATGA TGACCCACCT CCACGAACCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGT 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC GGAGGTGGTG 
TTGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCTCCAC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTCCATGCT GACTGATCGC GAGAACCAGT CGATCCTGAT CACTGGAGAA 
TCCGGTGCCG GGAAGACTGT GAACACAAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GCCAGTGGGG AGAAGAAGAA AGAGGAGAAG CAGCCAACAG GCAAAATGAG GGGAACGCTT 
GAGGATCAAA TCATCAGCGC CAACCCATTG CTGGAGGCCT TTGGAAATGC CAAGACTGTG 
AGGAACGACA ACTCCTCACG CTTTGGCAAA TTCATCCGGA TTCACTTTGG AGCCACAGGC 
AAACTGGCTT CTGCTGACAT TGAAACATAC CTGCTGGAGA AGTCCAGAGT CACCTTCCAA 
CTTAAGGCGG AAAGAAGCTA CCACATATTC TATCAGATCA TGTCCAACAA GAAGCCAGAG 
CTAATTGACA TGCTCCTCAT TACCACCAAC CCATATGACT ACCAATTTGT GAGTCAAGGT 
GAGATCACTG TTGCCAGCAT TAATGACCAG GAGGAGCTGA TGGCTACAGA TAGTGCCATT 
GACATCCTGG GCTTCACTGC TGATGAGAAA GCAGCCATTT ACAAGCTGAC GGGGGCTGTC 
ATGCACTATG GGAACTTGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCAGAT 
GGCACAGAAG TTGCTGACAA GGCTGCCTAC CTGATGGGTC TGAATTCAGC TGACCTGCTT 
AAGGCCCTCT GCTACCCTCG AGTCAAGGTG GGGAATGAAT ATGTGACCAA GGGTCAAACT 
GTGCAGCAGG TGCACAATTC AGTAGGTGCC CTGGCAAAGG CTGTCTATGA CAGAATGTTC 
TTGTGGATGG TTGTTCGCAT CAATCAACAG CTGGATACAA AGCAGCCCAG ACAGTACTTC 
ATTGGTGTCC TGGACATTGC TGGCTTTGAG ATCTTTGATT ACAACAGCCT GGAGCAGCTG 
TGCATCAACT TCACCAATGA GAAACTGCAA CAGTTCTTCA ACCACCACAT GTTCGTGCTG 
GAGCAGGAGG AGTACAAGAG GGAAGGAATC GAGTGGACGT TCATTGATTT TGGGATGGAC 
CTGGCTGCCT GCATTGAGCT CATTGAGAAG CCCATGGGCA TCTTCTCCAT CCTGGAAGAG 
GAGTGCATGT TCCCCAAGGC AACTGACACC TCTTTCAAGA ACAAGCTCTA TGATCAGCAT 
CTGGGCAAGT CCAGCAACTT CCAGAAGCCC AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TGCACTATGC TGGCACGGTG GACTACAACA TCACCGGCTG GCTGGAGAAG 
AACAAGGACC CCCTGAATGA AACTGTCATT GGGTTGTACC AGAAATCATC TGTGAAGACA 
CTGGCCTCAC TCTTTGCTTC TTACGGCGGA GAAGCAGAAG GTGGTGGTGG CAAGAAGGGT 
GGGAAGAAGA AGGGTTCTTC TTTCCAGACT GTCTCAGCTC TTTTCCGGGA GAACTTAAAC 
AAGCTGATGA CCAATCTGCG CAGCACTCAC CCACATTTTG TGAGATGCAT CATCCCCAAT 
GAAACAAAAA CACCTGGTGT CATGGAGCAT GAACTGGTGC TACACCAGCT TCGGTGCAAC 
GGTGTGTTGG AAGGGATCAG AATTTGCAGA AAAGGATTCC CCAGCAGAGT TGTCTATGCT 
GACTTTAAAC AGAGGTACAG GGTATTGAAT GCAAGTGCTG TCCCAGAGGG ACAGTTCATG 
GATAGCAAGA AAGCTTCTGA GAAGCTCCTT GGATCCATTG CTGTTGATCA CACACAATAT 
AAATTTGGTC ACACCAAGGT GTTCTTCAAA GCTGGACTGA TAGGTCTTCT GGAGGAGATG 
AGGGATGAGA AACTGGCACA GGTCATGACT CGCACACAAG CCAGATGCAG AGGCTTTCTG 
ATGAGAGTGG AGTACCAGAG AATGGTGGAG AGAAGGGAGT CCATCTTCTG TATCCAGTAC 
AACATTCGGG CATTCATGAA TGTCAAGCAC TGGCCTTGGA TGAAGCTGTG CTTCAAGATC 
AAGCCCTTGC TGAAGAGTGC AGAATCTGAG AAGGAGATGG CCAACATGAA ACAAGAGTTT 
GAGAAAACAA AGGAAGAGCT TGCAAAGTCT ACGACAGATA GGAAGGGGCT GGAGGAGAAA 
ATGGTGAAAC TGGTGCAGGA AAAAAATGAC CTGCAGCTCC AAGTGCAAGC TGAAGCGGAC 
GGCTTGGCTG ATGCTGAGGA AAGGTGTGAC CAGCTGATAA AAAACAAAAT CCAGCTGGAA 
GCAAAAGTTA AGGAGCTATG TGAAAGAGCA GAAGATGAAG AAGAGATGAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAATT GGAGGATGAA TGTTCAGAAC TCAAAAAAGA TATCGACGAT 
CTTGAGTTAA CACTGGCCAA GGTAGAGAAG GAAAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC AACCCTGGAT GAGACCATCG CTAAGCTGAC AAAAGAGAAG 
AAAGCCCTCC AAGAGGCCCA TCAGCAGACA CTGGATGACC TGCAGGCAGA AGAGGACAAA 
GTCAATACGC TGACCAAAGC TAAGACCAAG CTGGAGCAGC AAGTGGACGA TCTGGAAGGG 
TCCCTGGAGC AGGAGAAGAA GCTGCGCATG GACCTTGAGA GAGCTAAGAG GAAACTCGAA 
GGAGACCTGA AGCTGGCCCA TGACAGCATA ATGGATTTGG AAAATGATAA GCAGCAGCTG 
GATGAGAAAC TGAAGAAGAA AGACTTTGAA ATCAGCCAGA TCCAGAGCAA GATCGAGGAT 
GAGCAAGCCC TGGGCATGCA GCTACAGAAG AAGATCAAGG AGCTGCAGGC GCGTATTGAG 
GAACTGGAGG AGGAAATTGA GGCAGAGCGA ACCTCTCGGG CAAAAGCAGA GAAGCATCGT 
GCCGACCTCT CGAGGGAGCT GGAGGAGATC AGCGAGCGCC TGGAAGAAGC AGGAGGGGCC 
ACTGCAGCTC AGATGGAGAT GAACAAGAAG CGTGAGGCAG AATTTCAGAA GATGCGTCGC 
GACCTCGAAG AGGCCACGCT GCAGCACGAA GCCACGGCTG CCGCCCTGCG GAAGAAGCAC 
GCGGACAGCA CGGCCGAGCT CGGGGAGCAG ATCGACAACC TGCAGCGAGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GCTGAAGATG GAGATCGATG ACTTGGCCAG TAACATGGAG 
TCTGTCTCCA AAGCCAAGGC AAGTCTGGAA AAGATGTGTC GCACACTGGA AGATCAACTA 
AGTGAAATTA AGACAAAGGA AGAAGAGCAT CAGCGCATGA TCAACGACCT CAGTGCTCAA 
GCCAAGAACG CCCTGGCCCA CGCCTTGCAG TCTGCTCGCC ACGACTGTGA CTTGCTCCGG 
GAACAATATG AGGAGGAGCA GGAGGCCAAG GGGGAGCTGC AGCGTGCCCT GTCCAAGGCC 
AACAGCGAAG TGGCCCAGTG GAGAACCAAA TACGAGACGG ACGCTATTCA GCGCACGGAG 
GAGCTGGAGG AGGCCAAGAA GAAGCTGGCA CAGCGCCTGC AGGACGCAGA GGAACACGTT 
GAAGCTGTCA ATGCCAAATG TGCCTCCCTG GAAAAGACAA AGCAGAGGCT GCAGAATGAA 
GTGGAGGACC TGATGATTGA CGTGGAGCGA TCAAATGCTG CCTGCGCAGC TCTGGATAAG 
AAGCAGAAGA ACTTTGACAA GATCCTGGCA GAGTGGAAGC AGAAGTATGA GGAAACGCAG 
GCTGAGCTGG AAGCCTCCCA GAAGGAGTCT CGCTCTCTCA GCACGGAGCT CTTTAAGATG 
AAGAATGCCT ATGAGGAGTC CTTGGACCAC CTGGAAACGC TGAAGCGTGA GAACAAGAAC 
TTGCAGCAGG AGATTTCCGA CCTCACGGAG CAGATTGCTG AGGGAGGAAA GGCGATTCAT 
GAGCTGGAGA AAGTCAAGAA GCAGATTGAG CAGGAGAAAT CTGAAATCCA GGCTGCTCTG 
GAGGAAGCTG AGGCCTCCCT AGAACATGAA GAGGGGAAGA TCCTGCACCT CCAGCTTGAG 
CTCAACCAGG TGAAGTCGGA GATCGACAGG AAGATAGCAG AGAAAGATGA GGAGATCGAC 
CAGATGAAGA GAAACCACCT CAGAATCGTG GAGTCCTTGC AGAGCACCCT GGACGCTGAG 
ATCAGGAGCA GGAATGAAGC CCTGCGGCTG AAGAAGAAGA TGGAGGGAGA CCTGAATGAA 
ATGGAGATCC AGCTGGGCCA TGCCAACCGC GTGGCTGCAG AGGCACAAAA GAACCTGAGG 
AACACACAGG GAGTGCTCAA GGATACCCAG ATACACTTGG ATGATGCTCT CAGGACACAG 
GAGGACCTGA AGGAGCAGGT GGCCATGGTG GAGCGCAGAG CAAACCTGCT GCAGGCTGAA 
ATTGAGGAGC TGCGGGCAGC CCTGGAGCAG ACTGAGCGCT CAAGGAAAGT GGCTGAGCAG 
GAGCTTCTAG ATGCCACTGA ACGTGTGCAG CTCCTCCACA GCCAGAACAC CAGTTTGATC 
AACACCAAGA AAAAGCTGGA AACAGACATC ATGCAAATTC AGGGTGAAAT GGAGGATACA 
ATCCAGGAAG CGCGCAATGC TGAAGAGAAA GCCAAGAAGG CCATCACAGA TGCAGCCATG 
ATGGCAGAAG AGCTGAAGAA GGAGCAGGAC ACCAGCGCCC ACCTGGAAAG GATGAAGAAG 
AACCTGGACC AGACGGTGAA GGACCTGCAG CACCGTCTGG ATGAGGCTGA GCAGTTGGCT 
CTGAAGGGAG GCAAGAAGCA AATCCAGAAG CTGGAGGCCA GAGTGCGGGA GCTGGAAGGG 
GAGGTGGATG CTGAGCAGAA GCGCAGCGCT GAAGCCGTGA AGGGTGTGCG CAAGTACGAG 
AGGAGGGTGA AGGAGCTGAC CTACCAGTCT GAGGAAGACC GGAAGAATAT TCTGAGGCTC 
CAGGATCTGG TGGACAAGCT GCAAACGAAG GTGAAATCCT ACAAGAGACA AGCTGAGGAG 
GCTGAGGATC TCTCCAATGT CAATCTCATC AAGTTCCGCA AGATCCATCA TGACCTGGAG 
GAAGCTGAGG AACGGGCTGA CATTGCAGAG TCGCAGGTCA ACAAGCTCCG AGTGAAGAGT 
CGGGAGATTT ATAGGAAAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014812457.1
CDS	1..5664
Translation	MSSDAEMAIFGEAAVYLRKSEKERIEAQNKPFDAKSSVFVAHPKESFVKGTIQSKESGKVTVKTEGGETLTVKEDQIFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQSMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGEKKKEEKQPTGKMRGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIDMLLITTNPYDYQFVSQGEITVASINDQEELMATDSAIDILGFTADEKAAIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLMGLNSADLLKALCYPRVKVGNEYVTKGQTVQQVHNSVGALAKAVYDRMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSSNFQKPKPAKGKAEAHFSLVHYAGTVDYNITGWLEKNKDPLNETVIGLYQKSSVKTLASLFASYGGEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGVMEHELVLHQLRCNGVLEGIRICRKGFPSRVVYADFKQRYRVLNASAVPEGQFMDSKKASEKLLGSIAVDHTQYKFGHTKVFFKAGLIGLLEEMRDEKLAQVMTRTQARCRGFLMRVEYQRMVERRESIFCIQYNIRAFMNVKHWPWMKLCFKIKPLLKSAESEKEMANMKQEFEKTKEELAKSTTDRKGLEEKMVKLVQEKNDLQLQVQAEADGLADAEERCDQLIKNKIQLEAKVKELCERAEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMATLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAHDSIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISERLEEAGGATAAQMEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAKASLEKMCRTLEDQLSEIKTKEEEHQRMINDLSAQAKNALAHALQSARHDCDLLREQYEEEQEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERSNAACAALDKKQKNFDKILAEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSEIQAALEEAEASLEHEEGKILHLQLELNQVKSEIDRKIAEKDEEIDQMKRNHLRIVESLQSTLDAEIRSRNEALRLKKKMEGDLNEMEIQLGHANRVAAEAQKNLRNTQGVLKDTQIHLDDALRTQEDLKEQVAMVERRANLLQAEIEELRAALEQTERSRKVAEQELLDATERVQLLHSQNTSLINTKKKLETDIMQIQGEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNILRLQDLVDKLQTKVKSYKRQAEEAEDLSNVNLIKFRKIHHDLEEAEERADIAESQVNKLRVKSREIYRKN

Target ORF information:

RefSeq Version	XM_014956971.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax myosin heavy chain, skeletal muscle, adult-like (LOC106896755), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956971.1

ORF Insert Sequence:

ATGTCTTCAG ATGCTGAGAT GGCCATCTTT GGGGAGGCAG CTGTGTATCT CCGAAAGTCA 
GAAAAGGAGA GAATCGAGGC CCAGAACAAG CCTTTTGATG CCAAGTCATC TGTCTTTGTG 
GCACATCCTA AGGAATCTTT TGTGAAAGGG ACAATCCAGA GCAAAGAATC AGGGAAAGTC 
ACTGTCAAGA CAGAAGGTGG AGAGACCCTG ACCGTGAAGG AAGATCAAAT CTTCTCCATG 
AACCCTCCCA AGTATGATAA AATCGAGGAC ATGGCCATGA TGACCCACCT CCACGAACCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGT 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC GGAGGTGGTG 
TTGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCTCCAC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTCCATGCT GACTGATCGC GAGAACCAGT CGATCCTGAT CACTGGAGAA 
TCCGGTGCCG GGAAGACTGT GAACACAAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GCCAGTGGGG AGAAGAAGAA AGAGGAGAAG CAGCCAACAG GCAAAATGAG GGGAACGCTT 
GAGGATCAAA TCATCAGCGC CAACCCATTG CTGGAGGCCT TTGGAAATGC CAAGACTGTG 
AGGAACGACA ACTCCTCACG CTTTGGCAAA TTCATCCGGA TTCACTTTGG AGCCACAGGC 
AAACTGGCTT CTGCTGACAT TGAAACATAC CTGCTGGAGA AGTCCAGAGT CACCTTCCAA 
CTTAAGGCGG AAAGAAGCTA CCACATATTC TATCAGATCA TGTCCAACAA GAAGCCAGAG 
CTAATTGACA TGCTCCTCAT TACCACCAAC CCATATGACT ACCAATTTGT GAGTCAAGGT 
GAGATCACTG TTGCCAGCAT TAATGACCAG GAGGAGCTGA TGGCTACAGA TAGTGCCATT 
GACATCCTGG GCTTCACTGC TGATGAGAAA GCAGCCATTT ACAAGCTGAC GGGGGCTGTC 
ATGCACTATG GGAACTTGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCAGAT 
GGCACAGAAG TTGCTGACAA GGCTGCCTAC CTGATGGGTC TGAATTCAGC TGACCTGCTT 
AAGGCCCTCT GCTACCCTCG AGTCAAGGTG GGGAATGAAT ATGTGACCAA GGGTCAAACT 
GTGCAGCAGG TGCACAATTC AGTAGGTGCC CTGGCAAAGG CTGTCTATGA CAGAATGTTC 
TTGTGGATGG TTGTTCGCAT CAATCAACAG CTGGATACAA AGCAGCCCAG ACAGTACTTC 
ATTGGTGTCC TGGACATTGC TGGCTTTGAG ATCTTTGATT ACAACAGCCT GGAGCAGCTG 
TGCATCAACT TCACCAATGA GAAACTGCAA CAGTTCTTCA ACCACCACAT GTTCGTGCTG 
GAGCAGGAGG AGTACAAGAG GGAAGGAATC GAGTGGACGT TCATTGATTT TGGGATGGAC 
CTGGCTGCCT GCATTGAGCT CATTGAGAAG CCCATGGGCA TCTTCTCCAT CCTGGAAGAG 
GAGTGCATGT TCCCCAAGGC AACTGACACC TCTTTCAAGA ACAAGCTCTA TGATCAGCAT 
CTGGGCAAGT CCAGCAACTT CCAGAAGCCC AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TGCACTATGC TGGCACGGTG GACTACAACA TCACCGGCTG GCTGGAGAAG 
AACAAGGACC CCCTGAATGA AACTGTCATT GGGTTGTACC AGAAATCATC TGTGAAGACA 
CTGGCCTCAC TCTTTGCTTC TTACGGCGGA GAAGCAGAAG GTGGTGGTGG CAAGAAGGGT 
GGGAAGAAGA AGGGTTCTTC TTTCCAGACT GTCTCAGCTC TTTTCCGGGA GAACTTAAAC 
AAGCTGATGA CCAATCTGCG CAGCACTCAC CCACATTTTG TGAGATGCAT CATCCCCAAT 
GAAACAAAAA CACCTGGTGT CATGGAGCAT GAACTGGTGC TACACCAGCT TCGGTGCAAC 
GGTGTGTTGG AAGGGATCAG AATTTGCAGA AAAGGATTCC CCAGCAGAGT TGTCTATGCT 
GACTTTAAAC AGAGGTACAG GGTATTGAAT GCAAGTGCTG TCCCAGAGGG ACAGTTCATG 
GATAGCAAGA AAGCTTCTGA GAAGCTCCTT GGATCCATTG CTGTTGATCA CACACAATAT 
AAATTTGGTC ACACCAAGGT GTTCTTCAAA GCTGGACTGA TAGGTCTTCT GGAGGAGATG 
AGGGATGAGA AACTGGCACA GGTCATGACT CGCACACAAG CCAGATGCAG AGGCTTTCTG 
ATGAGAGTGG AGTACCAGAG AATGGTGGAG AGAAGGGAGT CCATCTTCTG TATCCAGTAC 
AACATTCGGG CATTCATGAA TGTCAAGCAC TGGCCTTGGA TGAAGCTGTG CTTCAAGATC 
AAGCCCTTGC TGAAGAGTGC AGAATCTGAG AAGGAGATGG CCAACATGAA ACAAGAGTTT 
GAGAAAACAA AGGAAGAGCT TGCAAAGTCT ACGACAGATA GGAAGGGGCT GGAGGAGAAA 
ATGGTGAAAC TGGTGCAGGA AAAAAATGAC CTGCAGCTCC AAGTGCAAGC TGAAGCGGAC 
GGCTTGGCTG ATGCTGAGGA AAGGTGTGAC CAGCTGATAA AAAACAAAAT CCAGCTGGAA 
GCAAAAGTTA AGGAGCTATG TGAAAGAGCA GAAGATGAAG AAGAGATGAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAATT GGAGGATGAA TGTTCAGAAC TCAAAAAAGA TATCGACGAT 
CTTGAGTTAA CACTGGCCAA GGTAGAGAAG GAAAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC AACCCTGGAT GAGACCATCG CTAAGCTGAC AAAAGAGAAG 
AAAGCCCTCC AAGAGGCCCA TCAGCAGACA CTGGATGACC TGCAGGCAGA AGAGGACAAA 
GTCAATACGC TGACCAAAGC TAAGACCAAG CTGGAGCAGC AAGTGGACGA TCTGGAAGGG 
TCCCTGGAGC AGGAGAAGAA GCTGCGCATG GACCTTGAGA GAGCTAAGAG GAAACTCGAA 
GGAGACCTGA AGCTGGCCCA TGACAGCATA ATGGATTTGG AAAATGATAA GCAGCAGCTG 
GATGAGAAAC TGAAGAAGAA AGACTTTGAA ATCAGCCAGA TCCAGAGCAA GATCGAGGAT 
GAGCAAGCCC TGGGCATGCA GCTACAGAAG AAGATCAAGG AGCTGCAGGC GCGTATTGAG 
GAACTGGAGG AGGAAATTGA GGCAGAGCGA ACCTCTCGGG CAAAAGCAGA GAAGCATCGT 
GCCGACCTCT CGAGGGAGCT GGAGGAGATC AGCGAGCGCC TGGAAGAAGC AGGAGGGGCC 
ACTGCAGCTC AGATGGAGAT GAACAAGAAG CGTGAGGCAG AATTTCAGAA GATGCGTCGC 
GACCTCGAAG AGGCCACGCT GCAGCACGAA GCCACGGCTG CCGCCCTGCG GAAGAAGCAC 
GCGGACAGCA CGGCCGAGCT CGGGGAGCAG ATCGACAACC TGCAGCGAGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GCTGAAGATG GAGATCGATG ACTTGGCCAG TAACATGGAG 
TCTGTCTCCA AAGCCAAGGC AAGTCTGGAA AAGATGTGTC GCACACTGGA AGATCAACTA 
AGTGAAATTA AGACAAAGGA AGAAGAGCAT CAGCGCATGA TCAACGACCT CAGTGCTCAA 
GCCAAGAACG CCCTGGCCCA CGCCTTGCAG TCTGCTCGCC ACGACTGTGA CTTGCTCCGG 
GAACAATATG AGGAGGAGCA GGAGGCCAAG GGGGAGCTGC AGCGTGCCCT GTCCAAGGCC 
AACAGCGAAG TGGCCCAGTG GAGAACCAAA TACGAGACGG ACGCTATTCA GCGCACGGAG 
GAGCTGGAGG AGGCCAAGAA GAAGCTGGCA CAGCGCCTGC AGGACGCAGA GGAACACGTT 
GAAGCTGTCA ATGCCAAATG TGCCTCCCTG GAAAAGACAA AGCAGAGGCT GCAGAATGAA 
GTGGAGGACC TGATGATTGA CGTGGAGCGA TCAAATGCTG CCTGCGCAGC TCTGGATAAG 
AAGCAGAAGA ACTTTGACAA GATCCTGGCA GAGTGGAAGC AGAAGTATGA GGAAACGCAG 
GCTGAGCTGG AAGCCTCCCA GAAGGAGTCT CGCTCTCTCA GCACGGAGCT CTTTAAGATG 
AAGAATGCCT ATGAGGAGTC CTTGGACCAC CTGGAAACGC TGAAGCGTGA GAACAAGAAC 
TTGCAGCAGG AGATTTCCGA CCTCACGGAG CAGATTGCTG AGGGAGGAAA GGCGATTCAT 
GAGCTGGAGA AAGTCAAGAA GCAGATTGAG CAGGAGAAAT CTGAAATCCA GGCTGCTCTG 
GAGGAAGCTG AGGCCTCCCT AGAACATGAA GAGGGGAAGA TCCTGCACCT CCAGCTTGAG 
CTCAACCAGG TGAAGTCGGA GATCGACAGG AAGATAGCAG AGAAAGATGA GGAGATCGAC 
CAGATGAAGA GAAACCACCT CAGAATCGTG GAGTCCTTGC AGAGCACCCT GGACGCTGAG 
ATCAGGAGCA GGAATGAAGC CCTGCGGCTG AAGAAGAAGA TGGAGGGAGA CCTGAATGAA 
ATGGAGATCC AGCTGGGCCA TGCCAACCGC GTGGCTGCAG AGGCACAAAA GAACCTGAGG 
AACACACAGG GAGTGCTCAA GGATACCCAG ATACACTTGG ATGATGCTCT CAGGACACAG 
GAGGACCTGA AGGAGCAGGT GGCCATGGTG GAGCGCAGAG CAAACCTGCT GCAGGCTGAA 
ATTGAGGAGC TGCGGGCAGC CCTGGAGCAG ACTGAGCGCT CAAGGAAAGT GGCTGAGCAG 
GAGCTTCTAG ATGCCACTGA ACGTGTGCAG CTCCTCCACA GCCAGAACAC CAGTTTGATC 
AACACCAAGA AAAAGCTGGA AACAGACATC ATGCAAATTC AGGGTGAAAT GGAGGATACA 
ATCCAGGAAG CGCGCAATGC TGAAGAGAAA GCCAAGAAGG CCATCACAGA TGCAGCCATG 
ATGGCAGAAG AGCTGAAGAA GGAGCAGGAC ACCAGCGCCC ACCTGGAAAG GATGAAGAAG 
AACCTGGACC AGACGGTGAA GGACCTGCAG CACCGTCTGG ATGAGGCTGA GCAGTTGGCT 
CTGAAGGGAG GCAAGAAGCA AATCCAGAAG CTGGAGGCCA GAGTGCGGGA GCTGGAAGGG 
GAGGTGGATG CTGAGCAGAA GCGCAGCGCT GAAGCCGTGA AGGGTGTGCG CAAGTACGAG 
AGGAGGGTGA AGGAGCTGAC CTACCAGTCT GAGGAAGACC GGAAGAATAT TCTGAGGCTC 
CAGGATCTGG TGGACAAGCT GCAAACGAAG GTGAAATCCT ACAAGAGACA AGCTGAGGAG 
GCTGAGGATC TCTCCAATGT CAATCTCATC AAGTTCCGCA AGATCCATCA TGACCTGGAG 
GAAGCTGAGG AACGGGCTGA CATTGCAGAG TCGCAGGTCA ACAAGCTCCG AGTGAAGAGT 
CGGGAGATTT ATAGGAAAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106896755 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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