ORF » Species Summary » Calidris pugnax » MAP4K4 cDNA ORF clone

MAP4K4 cDNA ORF clone, Calidris pugnax(ruff)

The following MAP4K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa76578 XM_014957746.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76579 XM_014957747.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76580 XM_014957748.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76581 XM_014957749.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76582 XM_014957751.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76583 XM_014957752.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76584 XM_014957753.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76585 XM_014957754.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76586 XM_014957755.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76587 XM_014957756.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCa76588 XM_014957757.1
Latest version!		 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MAP4K4 ( XM_014957746.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813232.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCa76578
    Clone ID Related Accession (Same CDS sequence) XM_014957746.1
    Accession Version XM_014957746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGGAGAAG TGGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA GTACAGTTGA CAAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTAA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG 
    ACAACTGTGA CTTCTGTGGT AGGCTTTTCT AGTGAAGCAA TGAGATCAGA GGCAATAAGG 
    CAAGACCCGA CGCGGAAAGG ATCAGTTGTC AATGTGAATC CCACGAATAC GCGGCCACAG 
    AGCGATACCC CAGAGATTCG CAAATACAAG AAGAGATTCA ATTCTGAGAT ACTCTGTGCT 
    GCCTTATGGG GAGTGAACTT GTTAGTGGGC ACTGAAAGTG GTCTGATGCT GCTAGACAGA 
    AGTGGTCAGG GGAAGGTTTA TCCGCTCATC AATCGAAGAC GATTCCAGCA AATGGATGTA 
    CTTGAAGGGC TGAATGTCTT GGTGACAATT TCTGGTAAGA AGAACAAGTT GCGAGTTTAC 
    TATTTGTCCT GGTTAAGAAA TAAGATCCTT CACAATGACC CAGAAGTAGA AAAGAAACAG 
    GGCTGGACAA CGGTGGGAGA CTTGGAAGGC TGTGTGCACT ATAAAGTTGT AAAATATGAA 
    AGAATCAAGT TCTTGGTAAT TGCATTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCA 
    AAGCCATACC ATAAATTTAT GGCCTTTAAG TCGTTTGGAG AACTGGTACA TAAGCCATTG 
    CTCGTGGATC TAACCGTAGA AGAGGGTCAG AGACTAAAAG TCATCTATGG CTCCTGCGCT 
    GGCTTCCACG CCGTCGATGT GGATTCAGGA TCGGTCTATG ATATTTATTT ACCAACACAT 
    ATCCAGAGCA GTATCCAACC TCACGCAATC ATCATTCTCC CAAACACGGA TGGGATGGAG 
    CTACTGGTCT GCTACGAGGA TGAAGGAGTT TACGTCAACA CGTATGGAAG GATCACCAAG 
    GATGTGGTTC TGCAGTGGGG AGAAATGCCA ACTTCAGTTG CATACATCCG ATCCAACCAG 
    ATTATGGGCT GGGGAGAAAA AGCTATTGAG ATACGATCGG TGGAAACTGG ACACTTGGAC 
    GGGGTGTTCA TGCACAAAAG GGCACAAAGA CTCAAGTTCT TATGCGAACG CAATGACAAG 
    GTTTTCTTTG CCTCCGTGCG GTCAGGTGGA AGCAGTCAAG TCTATTTCAT GACCCTTGGC 
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813232.1
    CDS123..4106
    Translation

    Target ORF information:

    RefSeq Version XM_014957746.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGGAGAAG TGGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA GTACAGTTGA CAAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTAA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG 
    ACAACTGTGA CTTCTGTGGT AGGCTTTTCT AGTGAAGCAA TGAGATCAGA GGCAATAAGG 
    CAAGACCCGA CGCGGAAAGG ATCAGTTGTC AATGTGAATC CCACGAATAC GCGGCCACAG 
    AGCGATACCC CAGAGATTCG CAAATACAAG AAGAGATTCA ATTCTGAGAT ACTCTGTGCT 
    GCCTTATGGG GAGTGAACTT GTTAGTGGGC ACTGAAAGTG GTCTGATGCT GCTAGACAGA 
    AGTGGTCAGG GGAAGGTTTA TCCGCTCATC AATCGAAGAC GATTCCAGCA AATGGATGTA 
    CTTGAAGGGC TGAATGTCTT GGTGACAATT TCTGGTAAGA AGAACAAGTT GCGAGTTTAC 
    TATTTGTCCT GGTTAAGAAA TAAGATCCTT CACAATGACC CAGAAGTAGA AAAGAAACAG 
    GGCTGGACAA CGGTGGGAGA CTTGGAAGGC TGTGTGCACT ATAAAGTTGT AAAATATGAA 
    AGAATCAAGT TCTTGGTAAT TGCATTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCA 
    AAGCCATACC ATAAATTTAT GGCCTTTAAG TCGTTTGGAG AACTGGTACA TAAGCCATTG 
    CTCGTGGATC TAACCGTAGA AGAGGGTCAG AGACTAAAAG TCATCTATGG CTCCTGCGCT 
    GGCTTCCACG CCGTCGATGT GGATTCAGGA TCGGTCTATG ATATTTATTT ACCAACACAT 
    ATCCAGAGCA GTATCCAACC TCACGCAATC ATCATTCTCC CAAACACGGA TGGGATGGAG 
    CTACTGGTCT GCTACGAGGA TGAAGGAGTT TACGTCAACA CGTATGGAAG GATCACCAAG 
    GATGTGGTTC TGCAGTGGGG AGAAATGCCA ACTTCAGTTG CATACATCCG ATCCAACCAG 
    ATTATGGGCT GGGGAGAAAA AGCTATTGAG ATACGATCGG TGGAAACTGG ACACTTGGAC 
    GGGGTGTTCA TGCACAAAAG GGCACAAAGA CTCAAGTTCT TATGCGAACG CAATGACAAG 
    GTTTTCTTTG CCTCCGTGCG GTCAGGTGGA AGCAGTCAAG TCTATTTCAT GACCCTTGGC 
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957747.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813233.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCa76579
    Clone ID Related Accession (Same CDS sequence) XM_014957747.1
    Accession Version XM_014957747.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGGAGAAG TGGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA GTACAGTTGA CAAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTAA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG 
    ACAACTGTGA CTTCTGTGGG CTTTTCTAGT GAAGCAATGA GATCAGAGGC AATAAGGCAA 
    GACCCGACGC GGAAAGGATC AGTTGTCAAT GTGAATCCCA CGAATACGCG GCCACAGAGC 
    GATACCCCAG AGATTCGCAA ATACAAGAAG AGATTCAATT CTGAGATACT CTGTGCTGCC 
    TTATGGGGAG TGAACTTGTT AGTGGGCACT GAAAGTGGTC TGATGCTGCT AGACAGAAGT 
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGACGAT TCCAGCAAAT GGATGTACTT 
    GAAGGGCTGA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAT 
    TTGTCCTGGT TAAGAAATAA GATCCTTCAC AATGACCCAG AAGTAGAAAA GAAACAGGGC 
    TGGACAACGG TGGGAGACTT GGAAGGCTGT GTGCACTATA AAGTTGTAAA ATATGAAAGA 
    ATCAAGTTCT TGGTAATTGC ATTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCAAAG 
    CCATACCATA AATTTATGGC CTTTAAGTCG TTTGGAGAAC TGGTACATAA GCCATTGCTC 
    GTGGATCTAA CCGTAGAAGA GGGTCAGAGA CTAAAAGTCA TCTATGGCTC CTGCGCTGGC 
    TTCCACGCCG TCGATGTGGA TTCAGGATCG GTCTATGATA TTTATTTACC AACACATATC 
    CAGAGCAGTA TCCAACCTCA CGCAATCATC ATTCTCCCAA ACACGGATGG GATGGAGCTA 
    CTGGTCTGCT ACGAGGATGA AGGAGTTTAC GTCAACACGT ATGGAAGGAT CACCAAGGAT 
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ACATCCGATC CAACCAGATT 
    ATGGGCTGGG GAGAAAAAGC TATTGAGATA CGATCGGTGG AAACTGGACA CTTGGACGGG 
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GCGAACGCAA TGACAAGGTT 
    TTCTTTGCCT CCGTGCGGTC AGGTGGAAGC AGTCAAGTCT ATTTCATGAC CCTTGGCAGG 
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813233.1
    CDS123..4103
    Translation

    Target ORF information:

    RefSeq Version XM_014957747.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGGAGAAG TGGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA GTACAGTTGA CAAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTAA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG 
    ACAACTGTGA CTTCTGTGGG CTTTTCTAGT GAAGCAATGA GATCAGAGGC AATAAGGCAA 
    GACCCGACGC GGAAAGGATC AGTTGTCAAT GTGAATCCCA CGAATACGCG GCCACAGAGC 
    GATACCCCAG AGATTCGCAA ATACAAGAAG AGATTCAATT CTGAGATACT CTGTGCTGCC 
    TTATGGGGAG TGAACTTGTT AGTGGGCACT GAAAGTGGTC TGATGCTGCT AGACAGAAGT 
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGACGAT TCCAGCAAAT GGATGTACTT 
    GAAGGGCTGA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAT 
    TTGTCCTGGT TAAGAAATAA GATCCTTCAC AATGACCCAG AAGTAGAAAA GAAACAGGGC 
    TGGACAACGG TGGGAGACTT GGAAGGCTGT GTGCACTATA AAGTTGTAAA ATATGAAAGA 
    ATCAAGTTCT TGGTAATTGC ATTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCAAAG 
    CCATACCATA AATTTATGGC CTTTAAGTCG TTTGGAGAAC TGGTACATAA GCCATTGCTC 
    GTGGATCTAA CCGTAGAAGA GGGTCAGAGA CTAAAAGTCA TCTATGGCTC CTGCGCTGGC 
    TTCCACGCCG TCGATGTGGA TTCAGGATCG GTCTATGATA TTTATTTACC AACACATATC 
    CAGAGCAGTA TCCAACCTCA CGCAATCATC ATTCTCCCAA ACACGGATGG GATGGAGCTA 
    CTGGTCTGCT ACGAGGATGA AGGAGTTTAC GTCAACACGT ATGGAAGGAT CACCAAGGAT 
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ACATCCGATC CAACCAGATT 
    ATGGGCTGGG GAGAAAAAGC TATTGAGATA CGATCGGTGG AAACTGGACA CTTGGACGGG 
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GCGAACGCAA TGACAAGGTT 
    TTCTTTGCCT CCGTGCGGTC AGGTGGAAGC AGTCAAGTCT ATTTCATGAC CCTTGGCAGG 
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957748.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813234.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OCa76580
    Clone ID Related Accession (Same CDS sequence) XM_014957748.1
    Accession Version XM_014957748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGTGGTC CCATCTGGCA TCTCTCAAGA ACAATGTTTC CCCTGTCTCG 
    CGATCCCATT CCTTCAGTGA CCCTTCTCCC AAATTTGCTC ATCACCATCT TCGTTCCCAG 
    GACCCATATC CACCTTCACG CAGTGAAGTG CTAAATCAGA GTTCTGACTC TAAGTCGGAG 
    GTACCCGACC CCACCCAAAA GGCTTGGGCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
    GTACCTGTGA GAACAACGTC CCGATCGCCA GTCCTGTCCC GCCGTGATTC TCCCCTGCAG 
    GGCAGTGGGC AGCAAAATAA CCAAGCAGGT CAAAGAAACT CCACGAGCAA TATAGAGCCT 
    CGTCTGCTGT GGGAGAGGGT GGAGAAGCTC GTACCAAGGC CAGGCAGTGG CAGTTCTTCG 
    GGTTCAAGCA ACTCCGGCTC ACAGCCTGGC TCCCACCCCG GCTCTCAGAG TGGGTCTGGA 
    GAGCGGTTCC GGATGAGATC ATCATCCAAA TCAGAAGGTT CGCCATCCCA GCGTCATGAA 
    AGTGCACCTA AAAAGCCTGA AGAGAAAAAG GAAGTCTTCA GACCTATCAA GCCTGCTGGA 
    GAAGTGGATT TAACTGCATT GGCAAAGGAA TTGCGAGCGG TAGAGGATGT GCGACCTCCA 
    CATAAAGTAA CGGATTATTC GTCCTCAAGT GAGGAGTCAG GGACGACAGA TGAGGAAGAT 
    GATGATATGG AACAGGAAGG AGCAGACGAA TCTACTTCTG GACCAGATGA TGTTCGAGCA 
    GTGTCAACAT TGAACTTGAG CAATGGTGAA ACAGAGTCAG TGAAAACCAT GATTGTCCAC 
    GATGATGTGG AGAGTGAACC TGCCTTGACT CCTTCTAAGG AGGGCACCTT AATTGTCCGA 
    CAGAGTACAG TTGACAAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCCGGTCGC 
    ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCC 
    TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG 
    CTAACTACTA ATGAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC 
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGGACAACT 
    GTGACTTCTG TGGTAGGCTT TTCTAGTGAA GCAATGAGAT CAGAGGCAAT AAGGCAAGAC 
    CCGACGCGGA AAGGATCAGT TGTCAATGTG AATCCCACGA ATACGCGGCC ACAGAGCGAT 
    ACCCCAGAGA TTCGCAAATA CAAGAAGAGA TTCAATTCTG AGATACTCTG TGCTGCCTTA 
    TGGGGAGTGA ACTTGTTAGT GGGCACTGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT 
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGACGATTCC AGCAAATGGA TGTACTTGAA 
    GGGCTGAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTATTTG 
    TCCTGGTTAA GAAATAAGAT CCTTCACAAT GACCCAGAAG TAGAAAAGAA ACAGGGCTGG 
    ACAACGGTGG GAGACTTGGA AGGCTGTGTG CACTATAAAG TTGTAAAATA TGAAAGAATC 
    AAGTTCTTGG TAATTGCATT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC ACCAAAGCCA 
    TACCATAAAT TTATGGCCTT TAAGTCGTTT GGAGAACTGG TACATAAGCC ATTGCTCGTG 
    GATCTAACCG TAGAAGAGGG TCAGAGACTA AAAGTCATCT ATGGCTCCTG CGCTGGCTTC 
    CACGCCGTCG ATGTGGATTC AGGATCGGTC TATGATATTT ATTTACCAAC ACATATCCAG 
    AGCAGTATCC AACCTCACGC AATCATCATT CTCCCAAACA CGGATGGGAT GGAGCTACTG 
    GTCTGCTACG AGGATGAAGG AGTTTACGTC AACACGTATG GAAGGATCAC CAAGGATGTG 
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATACA TCCGATCCAA CCAGATTATG 
    GGCTGGGGAG AAAAAGCTAT TGAGATACGA TCGGTGGAAA CTGGACACTT GGACGGGGTG 
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGCG AACGCAATGA CAAGGTTTTC 
    TTTGCCTCCG TGCGGTCAGG TGGAAGCAGT CAAGTCTATT TCATGACCCT TGGCAGGACT 
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813234.1
    CDS123..4100
    Translation

    Target ORF information:

    RefSeq Version XM_014957748.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGTGGTC CCATCTGGCA TCTCTCAAGA ACAATGTTTC CCCTGTCTCG 
    CGATCCCATT CCTTCAGTGA CCCTTCTCCC AAATTTGCTC ATCACCATCT TCGTTCCCAG 
    GACCCATATC CACCTTCACG CAGTGAAGTG CTAAATCAGA GTTCTGACTC TAAGTCGGAG 
    GTACCCGACC CCACCCAAAA GGCTTGGGCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
    GTACCTGTGA GAACAACGTC CCGATCGCCA GTCCTGTCCC GCCGTGATTC TCCCCTGCAG 
    GGCAGTGGGC AGCAAAATAA CCAAGCAGGT CAAAGAAACT CCACGAGCAA TATAGAGCCT 
    CGTCTGCTGT GGGAGAGGGT GGAGAAGCTC GTACCAAGGC CAGGCAGTGG CAGTTCTTCG 
    GGTTCAAGCA ACTCCGGCTC ACAGCCTGGC TCCCACCCCG GCTCTCAGAG TGGGTCTGGA 
    GAGCGGTTCC GGATGAGATC ATCATCCAAA TCAGAAGGTT CGCCATCCCA GCGTCATGAA 
    AGTGCACCTA AAAAGCCTGA AGAGAAAAAG GAAGTCTTCA GACCTATCAA GCCTGCTGGA 
    GAAGTGGATT TAACTGCATT GGCAAAGGAA TTGCGAGCGG TAGAGGATGT GCGACCTCCA 
    CATAAAGTAA CGGATTATTC GTCCTCAAGT GAGGAGTCAG GGACGACAGA TGAGGAAGAT 
    GATGATATGG AACAGGAAGG AGCAGACGAA TCTACTTCTG GACCAGATGA TGTTCGAGCA 
    GTGTCAACAT TGAACTTGAG CAATGGTGAA ACAGAGTCAG TGAAAACCAT GATTGTCCAC 
    GATGATGTGG AGAGTGAACC TGCCTTGACT CCTTCTAAGG AGGGCACCTT AATTGTCCGA 
    CAGAGTACAG TTGACAAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCCGGTCGC 
    ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCC 
    TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG 
    CTAACTACTA ATGAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC 
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGGACAACT 
    GTGACTTCTG TGGTAGGCTT TTCTAGTGAA GCAATGAGAT CAGAGGCAAT AAGGCAAGAC 
    CCGACGCGGA AAGGATCAGT TGTCAATGTG AATCCCACGA ATACGCGGCC ACAGAGCGAT 
    ACCCCAGAGA TTCGCAAATA CAAGAAGAGA TTCAATTCTG AGATACTCTG TGCTGCCTTA 
    TGGGGAGTGA ACTTGTTAGT GGGCACTGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT 
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGACGATTCC AGCAAATGGA TGTACTTGAA 
    GGGCTGAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTATTTG 
    TCCTGGTTAA GAAATAAGAT CCTTCACAAT GACCCAGAAG TAGAAAAGAA ACAGGGCTGG 
    ACAACGGTGG GAGACTTGGA AGGCTGTGTG CACTATAAAG TTGTAAAATA TGAAAGAATC 
    AAGTTCTTGG TAATTGCATT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC ACCAAAGCCA 
    TACCATAAAT TTATGGCCTT TAAGTCGTTT GGAGAACTGG TACATAAGCC ATTGCTCGTG 
    GATCTAACCG TAGAAGAGGG TCAGAGACTA AAAGTCATCT ATGGCTCCTG CGCTGGCTTC 
    CACGCCGTCG ATGTGGATTC AGGATCGGTC TATGATATTT ATTTACCAAC ACATATCCAG 
    AGCAGTATCC AACCTCACGC AATCATCATT CTCCCAAACA CGGATGGGAT GGAGCTACTG 
    GTCTGCTACG AGGATGAAGG AGTTTACGTC AACACGTATG GAAGGATCAC CAAGGATGTG 
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATACA TCCGATCCAA CCAGATTATG 
    GGCTGGGGAG AAAAAGCTAT TGAGATACGA TCGGTGGAAA CTGGACACTT GGACGGGGTG 
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGCG AACGCAATGA CAAGGTTTTC 
    TTTGCCTCCG TGCGGTCAGG TGGAAGCAGT CAAGTCTATT TCATGACCCT TGGCAGGACT 
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957749.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813235.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OCa76581
    Clone ID Related Accession (Same CDS sequence) XM_014957749.1
    Accession Version XM_014957749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGATTTAA CTGCATTGGC AAAGGAATTG CGAGCGGTAG AGGATGTGCG ACCTCCACAT 
    AAAGTAACGG ATTATTCGTC CTCAAGTGAG GAGTCAGGGA CGACAGATGA GGAAGATGAT 
    GATATGGAAC AGGAAGGAGC AGACGAATCT ACTTCTGGAC CAGATGATGT TCGAGCAGTG 
    TCAACATTGA ACTTGAGCAA TGGTGAAACA GAGTCAGTGA AAACCATGAT TGTCCACGAT 
    GATGTGGAGA GTGAACCTGC CTTGACTCCT TCTAAGGAGG GCACCTTAAT TGTCCGACAG 
    AGTACAGTTG ACAAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT 
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTA 
    ACTACTAATG AGACTCAGTC CGCTAGTAAC ACACTCCAGA AACACAAATC TTCCTCCTCC 
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACTGTG 
    ACTTCTGTGG TAGGCTTTTC TAGTGAAGCA ATGAGATCAG AGGCAATAAG GCAAGACCCG 
    ACGCGGAAAG GATCAGTTGT CAATGTGAAT CCCACGAATA CGCGGCCACA GAGCGATACC 
    CCAGAGATTC GCAAATACAA GAAGAGATTC AATTCTGAGA TACTCTGTGC TGCCTTATGG 
    GGAGTGAACT TGTTAGTGGG CACTGAAAGT GGTCTGATGC TGCTAGACAG AAGTGGTCAG 
    GGGAAGGTTT ATCCGCTCAT CAATCGAAGA CGATTCCAGC AAATGGATGT ACTTGAAGGG 
    CTGAATGTCT TGGTGACAAT TTCTGGTAAG AAGAACAAGT TGCGAGTTTA CTATTTGTCC 
    TGGTTAAGAA ATAAGATCCT TCACAATGAC CCAGAAGTAG AAAAGAAACA GGGCTGGACA 
    ACGGTGGGAG ACTTGGAAGG CTGTGTGCAC TATAAAGTTG TAAAATATGA AAGAATCAAG 
    TTCTTGGTAA TTGCATTGAA GAGTTCTGTG GAAGTCTATG CATGGGCACC AAAGCCATAC 
    CATAAATTTA TGGCCTTTAA GTCGTTTGGA GAACTGGTAC ATAAGCCATT GCTCGTGGAT 
    CTAACCGTAG AAGAGGGTCA GAGACTAAAA GTCATCTATG GCTCCTGCGC TGGCTTCCAC 
    GCCGTCGATG TGGATTCAGG ATCGGTCTAT GATATTTATT TACCAACACA TATCCAGAGC 
    AGTATCCAAC CTCACGCAAT CATCATTCTC CCAAACACGG ATGGGATGGA GCTACTGGTC 
    TGCTACGAGG ATGAAGGAGT TTACGTCAAC ACGTATGGAA GGATCACCAA GGATGTGGTT 
    CTGCAGTGGG GAGAAATGCC AACTTCAGTT GCATACATCC GATCCAACCA GATTATGGGC 
    TGGGGAGAAA AAGCTATTGA GATACGATCG GTGGAAACTG GACACTTGGA CGGGGTGTTC 
    ATGCACAAAA GGGCACAAAG ACTCAAGTTC TTATGCGAAC GCAATGACAA GGTTTTCTTT 
    GCCTCCGTGC GGTCAGGTGG AAGCAGTCAA GTCTATTTCA TGACCCTTGG CAGGACTTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813235.1
    CDS123..4097
    Translation

    Target ORF information:

    RefSeq Version XM_014957749.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGATTTAA CTGCATTGGC AAAGGAATTG CGAGCGGTAG AGGATGTGCG ACCTCCACAT 
    AAAGTAACGG ATTATTCGTC CTCAAGTGAG GAGTCAGGGA CGACAGATGA GGAAGATGAT 
    GATATGGAAC AGGAAGGAGC AGACGAATCT ACTTCTGGAC CAGATGATGT TCGAGCAGTG 
    TCAACATTGA ACTTGAGCAA TGGTGAAACA GAGTCAGTGA AAACCATGAT TGTCCACGAT 
    GATGTGGAGA GTGAACCTGC CTTGACTCCT TCTAAGGAGG GCACCTTAAT TGTCCGACAG 
    AGTACAGTTG ACAAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT 
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTA 
    ACTACTAATG AGACTCAGTC CGCTAGTAAC ACACTCCAGA AACACAAATC TTCCTCCTCC 
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACTGTG 
    ACTTCTGTGG TAGGCTTTTC TAGTGAAGCA ATGAGATCAG AGGCAATAAG GCAAGACCCG 
    ACGCGGAAAG GATCAGTTGT CAATGTGAAT CCCACGAATA CGCGGCCACA GAGCGATACC 
    CCAGAGATTC GCAAATACAA GAAGAGATTC AATTCTGAGA TACTCTGTGC TGCCTTATGG 
    GGAGTGAACT TGTTAGTGGG CACTGAAAGT GGTCTGATGC TGCTAGACAG AAGTGGTCAG 
    GGGAAGGTTT ATCCGCTCAT CAATCGAAGA CGATTCCAGC AAATGGATGT ACTTGAAGGG 
    CTGAATGTCT TGGTGACAAT TTCTGGTAAG AAGAACAAGT TGCGAGTTTA CTATTTGTCC 
    TGGTTAAGAA ATAAGATCCT TCACAATGAC CCAGAAGTAG AAAAGAAACA GGGCTGGACA 
    ACGGTGGGAG ACTTGGAAGG CTGTGTGCAC TATAAAGTTG TAAAATATGA AAGAATCAAG 
    TTCTTGGTAA TTGCATTGAA GAGTTCTGTG GAAGTCTATG CATGGGCACC AAAGCCATAC 
    CATAAATTTA TGGCCTTTAA GTCGTTTGGA GAACTGGTAC ATAAGCCATT GCTCGTGGAT 
    CTAACCGTAG AAGAGGGTCA GAGACTAAAA GTCATCTATG GCTCCTGCGC TGGCTTCCAC 
    GCCGTCGATG TGGATTCAGG ATCGGTCTAT GATATTTATT TACCAACACA TATCCAGAGC 
    AGTATCCAAC CTCACGCAAT CATCATTCTC CCAAACACGG ATGGGATGGA GCTACTGGTC 
    TGCTACGAGG ATGAAGGAGT TTACGTCAAC ACGTATGGAA GGATCACCAA GGATGTGGTT 
    CTGCAGTGGG GAGAAATGCC AACTTCAGTT GCATACATCC GATCCAACCA GATTATGGGC 
    TGGGGAGAAA AAGCTATTGA GATACGATCG GTGGAAACTG GACACTTGGA CGGGGTGTTC 
    ATGCACAAAA GGGCACAAAG ACTCAAGTTC TTATGCGAAC GCAATGACAA GGTTTTCTTT 
    GCCTCCGTGC GGTCAGGTGG AAGCAGTCAA GTCTATTTCA TGACCCTTGG CAGGACTTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957751.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813237.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OCa76582
    Clone ID Related Accession (Same CDS sequence) XM_014957751.1
    Accession Version XM_014957751.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAATACAGAT GGCGAGAGAT GGAGGAACAC 
    CGACAGGCGG AGCGACTCCA GCGCCAGTTG CAGCAGGAGC AGGCCTACCT CCTGTCGCTG 
    CAGCACGATC ACAGGCGGCA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA GCAAGAAAGA 
    AATAAACAAA GCTATCATAC CCCTGAGCCA AAATCCCACT ATGAGCCTGC TGAAAGAGCG 
    CGTGAGGTGG AGGAAAGATT TAGAAAAACT AATCAGGGCT CCCCCGAAGC ACAGTCTAAA 
    CAGATGGGCA AAGTGTTGGA GCCACCAGTG CCTTCAAGAT CAGAGTCCTT TTCCAATGGA 
    AACTCAGAAC CTGCTCAGCC TGCCCTGCAG AGGCCGATGG AGCCCCAGGT ACAGTGGTCC 
    CATCTGGCAT CTCTCAAGAA CAATGTTTCC CCTGTCTCGC GATCCCATTC CTTCAGTGAC 
    CCTTCTCCCA AATTTGCTCA TCACCATCTT CGTTCCCAGG ACCCATATCC ACCTTCACGC 
    AGTGAAGTGC TAAATCAGAG TTCTGACTCT AAGTCGGAGG TACCCGACCC CACCCAAAAG 
    GCTTGGGCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TACCTGTGAG AACAACGTCC 
    CGATCGCCAG TCCTGTCCCG CCGTGATTCT CCCCTGCAGG GCAGTGGGCA GCAAAATAAC 
    CAAGCAGGTC AAAGAAACTC CACGAGCAAT ATAGAGCCTC GTCTGCTGTG GGAGAGGGTG 
    GAGAAGCTCG TACCAAGGCC AGGCAGTGGC AGTTCTTCGG GTTCAAGCAA CTCCGGCTCA 
    CAGCCTGGCT CCCACCCCGG CTCTCAGAGT GGGTCTGGAG AGCGGTTCCG GATGAGATCA 
    TCATCCAAAT CAGAAGGTTC GCCATCCCAG CGTCATGAAA GTGCACCTAA AAAGCCTGAA 
    GAGAAAAAGG AAGTCTTCAG ACCTATCAAG CCTGCTGGAG AAGTGGATTT AACTGCATTG 
    GCAAAGGAAT TGCGAGCGGT AGAGGATGTG CGACCTCCAC ATAAAGTAAC GGATTATTCG 
    TCCTCAAGTG AGGAGTCAGG GACGACAGAT GAGGAAGATG ATGATATGGA ACAGGAAGGA 
    GCAGACGAAT CTACTTCTGG ACCAGATGAT GTTCGAGCAG TGTCAACATT GAACTTGAGC 
    AATGGTGAAA CAGAGTCAGT GAAAACCATG ATTGTCCACG ATGATGTGGA GAGTGAACCT 
    GCCTTGACTC CTTCTAAGGA GGGCACCTTA ATTGTCCGAC AGAGTACAGT TGACAAAAAG 
    CGTGCCAGCC ATCATGAGAG CAATGGCTTT GCCGGTCGCA TTCACCTCTT GCCAGATCTC 
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CCTCCCCATC CTCCAGCCAG 
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TAACTACTAA TGAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGT GGGACAACTG TGACTTCTGT GGTAGGCTTT 
    TCTAGTGAAG CAATGAGATC AGAGGCAATA AGGCAAGACC CGACGCGGAA AGGATCAGTT 
    GTCAATGTGA ATCCCACGAA TACGCGGCCA CAGAGCGATA CCCCAGAGAT TCGCAAATAC 
    AAGAAGAGAT TCAATTCTGA GATACTCTGT GCTGCCTTAT GGGGAGTGAA CTTGTTAGTG 
    GGCACTGAAA GTGGTCTGAT GCTGCTAGAC AGAAGTGGTC AGGGGAAGGT TTATCCGCTC 
    ATCAATCGAA GACGATTCCA GCAAATGGAT GTACTTGAAG GGCTGAATGT CTTGGTGACA 
    ATTTCTGGTA AGAAGAACAA GTTGCGAGTT TACTATTTGT CCTGGTTAAG AAATAAGATC 
    CTTCACAATG ACCCAGAAGT AGAAAAGAAA CAGGGCTGGA CAACGGTGGG AGACTTGGAA 
    GGCTGTGTGC ACTATAAAGT TGTAAAATAT GAAAGAATCA AGTTCTTGGT AATTGCATTG 
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCAAAGCCAT ACCATAAATT TATGGCCTTT 
    AAGTCGTTTG GAGAACTGGT ACATAAGCCA TTGCTCGTGG ATCTAACCGT AGAAGAGGGT 
    CAGAGACTAA AAGTCATCTA TGGCTCCTGC GCTGGCTTCC ACGCCGTCGA TGTGGATTCA 
    GGATCGGTCT ATGATATTTA TTTACCAACA CATATCCAGA GCAGTATCCA ACCTCACGCA 
    ATCATCATTC TCCCAAACAC GGATGGGATG GAGCTACTGG TCTGCTACGA GGATGAAGGA 
    GTTTACGTCA ACACGTATGG AAGGATCACC AAGGATGTGG TTCTGCAGTG GGGAGAAATG 
    CCAACTTCAG TTGCATACAT CCGATCCAAC CAGATTATGG GCTGGGGAGA AAAAGCTATT 
    GAGATACGAT CGGTGGAAAC TGGACACTTG GACGGGGTGT TCATGCACAA AAGGGCACAA 
    AGACTCAAGT TCTTATGCGA ACGCAATGAC AAGGTTTTCT TTGCCTCCGT GCGGTCAGGT 
    GGAAGCAGTC AAGTCTATTT CATGACCCTT GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813237.1
    CDS123..4019
    Translation

    Target ORF information:

    RefSeq Version XM_014957751.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAATACAGAT GGCGAGAGAT GGAGGAACAC 
    CGACAGGCGG AGCGACTCCA GCGCCAGTTG CAGCAGGAGC AGGCCTACCT CCTGTCGCTG 
    CAGCACGATC ACAGGCGGCA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA GCAAGAAAGA 
    AATAAACAAA GCTATCATAC CCCTGAGCCA AAATCCCACT ATGAGCCTGC TGAAAGAGCG 
    CGTGAGGTGG AGGAAAGATT TAGAAAAACT AATCAGGGCT CCCCCGAAGC ACAGTCTAAA 
    CAGATGGGCA AAGTGTTGGA GCCACCAGTG CCTTCAAGAT CAGAGTCCTT TTCCAATGGA 
    AACTCAGAAC CTGCTCAGCC TGCCCTGCAG AGGCCGATGG AGCCCCAGGT ACAGTGGTCC 
    CATCTGGCAT CTCTCAAGAA CAATGTTTCC CCTGTCTCGC GATCCCATTC CTTCAGTGAC 
    CCTTCTCCCA AATTTGCTCA TCACCATCTT CGTTCCCAGG ACCCATATCC ACCTTCACGC 
    AGTGAAGTGC TAAATCAGAG TTCTGACTCT AAGTCGGAGG TACCCGACCC CACCCAAAAG 
    GCTTGGGCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TACCTGTGAG AACAACGTCC 
    CGATCGCCAG TCCTGTCCCG CCGTGATTCT CCCCTGCAGG GCAGTGGGCA GCAAAATAAC 
    CAAGCAGGTC AAAGAAACTC CACGAGCAAT ATAGAGCCTC GTCTGCTGTG GGAGAGGGTG 
    GAGAAGCTCG TACCAAGGCC AGGCAGTGGC AGTTCTTCGG GTTCAAGCAA CTCCGGCTCA 
    CAGCCTGGCT CCCACCCCGG CTCTCAGAGT GGGTCTGGAG AGCGGTTCCG GATGAGATCA 
    TCATCCAAAT CAGAAGGTTC GCCATCCCAG CGTCATGAAA GTGCACCTAA AAAGCCTGAA 
    GAGAAAAAGG AAGTCTTCAG ACCTATCAAG CCTGCTGGAG AAGTGGATTT AACTGCATTG 
    GCAAAGGAAT TGCGAGCGGT AGAGGATGTG CGACCTCCAC ATAAAGTAAC GGATTATTCG 
    TCCTCAAGTG AGGAGTCAGG GACGACAGAT GAGGAAGATG ATGATATGGA ACAGGAAGGA 
    GCAGACGAAT CTACTTCTGG ACCAGATGAT GTTCGAGCAG TGTCAACATT GAACTTGAGC 
    AATGGTGAAA CAGAGTCAGT GAAAACCATG ATTGTCCACG ATGATGTGGA GAGTGAACCT 
    GCCTTGACTC CTTCTAAGGA GGGCACCTTA ATTGTCCGAC AGAGTACAGT TGACAAAAAG 
    CGTGCCAGCC ATCATGAGAG CAATGGCTTT GCCGGTCGCA TTCACCTCTT GCCAGATCTC 
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CCTCCCCATC CTCCAGCCAG 
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TAACTACTAA TGAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGT GGGACAACTG TGACTTCTGT GGTAGGCTTT 
    TCTAGTGAAG CAATGAGATC AGAGGCAATA AGGCAAGACC CGACGCGGAA AGGATCAGTT 
    GTCAATGTGA ATCCCACGAA TACGCGGCCA CAGAGCGATA CCCCAGAGAT TCGCAAATAC 
    AAGAAGAGAT TCAATTCTGA GATACTCTGT GCTGCCTTAT GGGGAGTGAA CTTGTTAGTG 
    GGCACTGAAA GTGGTCTGAT GCTGCTAGAC AGAAGTGGTC AGGGGAAGGT TTATCCGCTC 
    ATCAATCGAA GACGATTCCA GCAAATGGAT GTACTTGAAG GGCTGAATGT CTTGGTGACA 
    ATTTCTGGTA AGAAGAACAA GTTGCGAGTT TACTATTTGT CCTGGTTAAG AAATAAGATC 
    CTTCACAATG ACCCAGAAGT AGAAAAGAAA CAGGGCTGGA CAACGGTGGG AGACTTGGAA 
    GGCTGTGTGC ACTATAAAGT TGTAAAATAT GAAAGAATCA AGTTCTTGGT AATTGCATTG 
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCAAAGCCAT ACCATAAATT TATGGCCTTT 
    AAGTCGTTTG GAGAACTGGT ACATAAGCCA TTGCTCGTGG ATCTAACCGT AGAAGAGGGT 
    CAGAGACTAA AAGTCATCTA TGGCTCCTGC GCTGGCTTCC ACGCCGTCGA TGTGGATTCA 
    GGATCGGTCT ATGATATTTA TTTACCAACA CATATCCAGA GCAGTATCCA ACCTCACGCA 
    ATCATCATTC TCCCAAACAC GGATGGGATG GAGCTACTGG TCTGCTACGA GGATGAAGGA 
    GTTTACGTCA ACACGTATGG AAGGATCACC AAGGATGTGG TTCTGCAGTG GGGAGAAATG 
    CCAACTTCAG TTGCATACAT CCGATCCAAC CAGATTATGG GCTGGGGAGA AAAAGCTATT 
    GAGATACGAT CGGTGGAAAC TGGACACTTG GACGGGGTGT TCATGCACAA AAGGGCACAA 
    AGACTCAAGT TCTTATGCGA ACGCAATGAC AAGGTTTTCT TTGCCTCCGT GCGGTCAGGT 
    GGAAGCAGTC AAGTCTATTT CATGACCCTT GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957752.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813238.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OCa76583
    Clone ID Related Accession (Same CDS sequence) XM_014957752.1
    Accession Version XM_014957752.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGGAGAAG TGGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACTGTGACT 
    TCTGTGGTAG GCTTTTCTAG TGAAGCAATG AGATCAGAGG CAATAAGGCA AGACCCGACG 
    CGGAAAGGAT CAGTTGTCAA TGTGAATCCC ACGAATACGC GGCCACAGAG CGATACCCCA 
    GAGATTCGCA AATACAAGAA GAGATTCAAT TCTGAGATAC TCTGTGCTGC CTTATGGGGA 
    GTGAACTTGT TAGTGGGCAC TGAAAGTGGT CTGATGCTGC TAGACAGAAG TGGTCAGGGG 
    AAGGTTTATC CGCTCATCAA TCGAAGACGA TTCCAGCAAA TGGATGTACT TGAAGGGCTG 
    AATGTCTTGG TGACAATTTC TGGTAAGAAG AACAAGTTGC GAGTTTACTA TTTGTCCTGG 
    TTAAGAAATA AGATCCTTCA CAATGACCCA GAAGTAGAAA AGAAACAGGG CTGGACAACG 
    GTGGGAGACT TGGAAGGCTG TGTGCACTAT AAAGTTGTAA AATATGAAAG AATCAAGTTC 
    TTGGTAATTG CATTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCACCAAA GCCATACCAT 
    AAATTTATGG CCTTTAAGTC GTTTGGAGAA CTGGTACATA AGCCATTGCT CGTGGATCTA 
    ACCGTAGAAG AGGGTCAGAG ACTAAAAGTC ATCTATGGCT CCTGCGCTGG CTTCCACGCC 
    GTCGATGTGG ATTCAGGATC GGTCTATGAT ATTTATTTAC CAACACATAT CCAGAGCAGT 
    ATCCAACCTC ACGCAATCAT CATTCTCCCA AACACGGATG GGATGGAGCT ACTGGTCTGC 
    TACGAGGATG AAGGAGTTTA CGTCAACACG TATGGAAGGA TCACCAAGGA TGTGGTTCTG 
    CAGTGGGGAG AAATGCCAAC TTCAGTTGCA TACATCCGAT CCAACCAGAT TATGGGCTGG 
    GGAGAAAAAG CTATTGAGAT ACGATCGGTG GAAACTGGAC ACTTGGACGG GGTGTTCATG 
    CACAAAAGGG CACAAAGACT CAAGTTCTTA TGCGAACGCA ATGACAAGGT TTTCTTTGCC 
    TCCGTGCGGT CAGGTGGAAG CAGTCAAGTC TATTTCATGA CCCTTGGCAG GACTTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813238.1
    CDS123..3914
    Translation

    Target ORF information:

    RefSeq Version XM_014957752.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGGAGAAG TGGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACTGTGACT 
    TCTGTGGTAG GCTTTTCTAG TGAAGCAATG AGATCAGAGG CAATAAGGCA AGACCCGACG 
    CGGAAAGGAT CAGTTGTCAA TGTGAATCCC ACGAATACGC GGCCACAGAG CGATACCCCA 
    GAGATTCGCA AATACAAGAA GAGATTCAAT TCTGAGATAC TCTGTGCTGC CTTATGGGGA 
    GTGAACTTGT TAGTGGGCAC TGAAAGTGGT CTGATGCTGC TAGACAGAAG TGGTCAGGGG 
    AAGGTTTATC CGCTCATCAA TCGAAGACGA TTCCAGCAAA TGGATGTACT TGAAGGGCTG 
    AATGTCTTGG TGACAATTTC TGGTAAGAAG AACAAGTTGC GAGTTTACTA TTTGTCCTGG 
    TTAAGAAATA AGATCCTTCA CAATGACCCA GAAGTAGAAA AGAAACAGGG CTGGACAACG 
    GTGGGAGACT TGGAAGGCTG TGTGCACTAT AAAGTTGTAA AATATGAAAG AATCAAGTTC 
    TTGGTAATTG CATTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCACCAAA GCCATACCAT 
    AAATTTATGG CCTTTAAGTC GTTTGGAGAA CTGGTACATA AGCCATTGCT CGTGGATCTA 
    ACCGTAGAAG AGGGTCAGAG ACTAAAAGTC ATCTATGGCT CCTGCGCTGG CTTCCACGCC 
    GTCGATGTGG ATTCAGGATC GGTCTATGAT ATTTATTTAC CAACACATAT CCAGAGCAGT 
    ATCCAACCTC ACGCAATCAT CATTCTCCCA AACACGGATG GGATGGAGCT ACTGGTCTGC 
    TACGAGGATG AAGGAGTTTA CGTCAACACG TATGGAAGGA TCACCAAGGA TGTGGTTCTG 
    CAGTGGGGAG AAATGCCAAC TTCAGTTGCA TACATCCGAT CCAACCAGAT TATGGGCTGG 
    GGAGAAAAAG CTATTGAGAT ACGATCGGTG GAAACTGGAC ACTTGGACGG GGTGTTCATG 
    CACAAAAGGG CACAAAGACT CAAGTTCTTA TGCGAACGCA ATGACAAGGT TTTCTTTGCC 
    TCCGTGCGGT CAGGTGGAAG CAGTCAAGTC TATTTCATGA CCCTTGGCAG GACTTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957753.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813239.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OCa76584
    Clone ID Related Accession (Same CDS sequence) XM_014957753.1
    Accession Version XM_014957753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGGAGAAG TGGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACTGTGACT 
    TCTGTGGGCT TTTCTAGTGA AGCAATGAGA TCAGAGGCAA TAAGGCAAGA CCCGACGCGG 
    AAAGGATCAG TTGTCAATGT GAATCCCACG AATACGCGGC CACAGAGCGA TACCCCAGAG 
    ATTCGCAAAT ACAAGAAGAG ATTCAATTCT GAGATACTCT GTGCTGCCTT ATGGGGAGTG 
    AACTTGTTAG TGGGCACTGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG 
    GTTTATCCGC TCATCAATCG AAGACGATTC CAGCAAATGG ATGTACTTGA AGGGCTGAAT 
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTATTT GTCCTGGTTA 
    AGAAATAAGA TCCTTCACAA TGACCCAGAA GTAGAAAAGA AACAGGGCTG GACAACGGTG 
    GGAGACTTGG AAGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAAAGAAT CAAGTTCTTG 
    GTAATTGCAT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCAAAGCC ATACCATAAA 
    TTTATGGCCT TTAAGTCGTT TGGAGAACTG GTACATAAGC CATTGCTCGT GGATCTAACC 
    GTAGAAGAGG GTCAGAGACT AAAAGTCATC TATGGCTCCT GCGCTGGCTT CCACGCCGTC 
    GATGTGGATT CAGGATCGGT CTATGATATT TATTTACCAA CACATATCCA GAGCAGTATC 
    CAACCTCACG CAATCATCAT TCTCCCAAAC ACGGATGGGA TGGAGCTACT GGTCTGCTAC 
    GAGGATGAAG GAGTTTACGT CAACACGTAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG 
    TGGGGAGAAA TGCCAACTTC AGTTGCATAC ATCCGATCCA ACCAGATTAT GGGCTGGGGA 
    GAAAAAGCTA TTGAGATACG ATCGGTGGAA ACTGGACACT TGGACGGGGT GTTCATGCAC 
    AAAAGGGCAC AAAGACTCAA GTTCTTATGC GAACGCAATG ACAAGGTTTT CTTTGCCTCC 
    GTGCGGTCAG GTGGAAGCAG TCAAGTCTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813239.1
    CDS123..3911
    Translation

    Target ORF information:

    RefSeq Version XM_014957753.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACA GTGGTCCCAT CTGGCATCTC TCAAGAACAA TGTTTCCCCT 
    GTCTCGCGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TTGCTCATCA CCATCTTCGT 
    TCCCAGGACC CATATCCACC TTCACGCAGT GAAGTGCTAA ATCAGAGTTC TGACTCTAAG 
    TCGGAGGTAC CCGACCCCAC CCAAAAGGCT TGGGCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTAC CTGTGAGAAC AACGTCCCGA TCGCCAGTCC TGTCCCGCCG TGATTCTCCC 
    CTGCAGGGCA GTGGGCAGCA AAATAACCAA GCAGGTCAAA GAAACTCCAC GAGCAATATA 
    GAGCCTCGTC TGCTGTGGGA GAGGGTGGAG AAGCTCGTAC CAAGGCCAGG CAGTGGCAGT 
    TCTTCGGGTT CAAGCAACTC CGGCTCACAG CCTGGCTCCC ACCCCGGCTC TCAGAGTGGG 
    TCTGGAGAGC GGTTCCGGAT GAGATCATCA TCCAAATCAG AAGGTTCGCC ATCCCAGCGT 
    CATGAAAGTG CACCTAAAAA GCCTGAAGAG AAAAAGGAAG TCTTCAGACC TATCAAGCCT 
    GCTGGAGAAG TGGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACTGTGACT 
    TCTGTGGGCT TTTCTAGTGA AGCAATGAGA TCAGAGGCAA TAAGGCAAGA CCCGACGCGG 
    AAAGGATCAG TTGTCAATGT GAATCCCACG AATACGCGGC CACAGAGCGA TACCCCAGAG 
    ATTCGCAAAT ACAAGAAGAG ATTCAATTCT GAGATACTCT GTGCTGCCTT ATGGGGAGTG 
    AACTTGTTAG TGGGCACTGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG 
    GTTTATCCGC TCATCAATCG AAGACGATTC CAGCAAATGG ATGTACTTGA AGGGCTGAAT 
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTATTT GTCCTGGTTA 
    AGAAATAAGA TCCTTCACAA TGACCCAGAA GTAGAAAAGA AACAGGGCTG GACAACGGTG 
    GGAGACTTGG AAGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAAAGAAT CAAGTTCTTG 
    GTAATTGCAT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCAAAGCC ATACCATAAA 
    TTTATGGCCT TTAAGTCGTT TGGAGAACTG GTACATAAGC CATTGCTCGT GGATCTAACC 
    GTAGAAGAGG GTCAGAGACT AAAAGTCATC TATGGCTCCT GCGCTGGCTT CCACGCCGTC 
    GATGTGGATT CAGGATCGGT CTATGATATT TATTTACCAA CACATATCCA GAGCAGTATC 
    CAACCTCACG CAATCATCAT TCTCCCAAAC ACGGATGGGA TGGAGCTACT GGTCTGCTAC 
    GAGGATGAAG GAGTTTACGT CAACACGTAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG 
    TGGGGAGAAA TGCCAACTTC AGTTGCATAC ATCCGATCCA ACCAGATTAT GGGCTGGGGA 
    GAAAAAGCTA TTGAGATACG ATCGGTGGAA ACTGGACACT TGGACGGGGT GTTCATGCAC 
    AAAAGGGCAC AAAGACTCAA GTTCTTATGC GAACGCAATG ACAAGGTTTT CTTTGCCTCC 
    GTGCGGTCAG GTGGAAGCAG TCAAGTCTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957754.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813240.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OCa76585
    Clone ID Related Accession (Same CDS sequence) XM_014957754.1
    Accession Version XM_014957754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACC TGTGAGAACA ACGTCCCGAT CGCCAGTCCT GTCCCGCCGT 
    GATTCTCCCC TGCAGGGCAG TGGGCAGCAA AATAACCAAG CAGGTCAAAG AAACTCCACG 
    AGCAATATAG AGCCTCGTCT GCTGTGGGAG AGGGTGGAGA AGCTCGTACC AAGGCCAGGC 
    AGTGGCAGTT CTTCGGGTTC AAGCAACTCC GGCTCACAGC CTGGCTCCCA CCCCGGCTCT 
    CAGAGTGGGT CTGGAGAGCG GTTCCGGATG AGATCATCAT CCAAATCAGA AGGTTCGCCA 
    TCCCAGCGTC ATGAAAGTGC ACCTAAAAAG CCTGAAGAGA AAAAGGAAGT CTTCAGACCT 
    ATCAAGCCTG CTGGAGAAGT GGATTTAACT GCATTGGCAA AGGAATTGCG AGCGGTAGAG 
    GATGTGCGAC CTCCACATAA AGTAACGGAT TATTCGTCCT CAAGTGAGGA GTCAGGGACG 
    ACAGATGAGG AAGATGATGA TATGGAACAG GAAGGAGCAG ACGAATCTAC TTCTGGACCA 
    GATGATGTTC GAGCAGTGTC AACATTGAAC TTGAGCAATG GTGAAACAGA GTCAGTGAAA 
    ACCATGATTG TCCACGATGA TGTGGAGAGT GAACCTGCCT TGACTCCTTC TAAGGAGGGC 
    ACCTTAATTG TCCGACAGAG TACAGTTGAC AAAAAGCGTG CCAGCCATCA TGAGAGCAAT 
    GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
    ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
    ACACCCCAGG ACAAGCTAAC TACTAATGAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGTGGGA CAACTGTGAC TTCTGTGGTA GGCTTTTCTA GTGAAGCAAT GAGATCAGAG 
    GCAATAAGGC AAGACCCGAC GCGGAAAGGA TCAGTTGTCA ATGTGAATCC CACGAATACG 
    CGGCCACAGA GCGATACCCC AGAGATTCGC AAATACAAGA AGAGATTCAA TTCTGAGATA 
    CTCTGTGCTG CCTTATGGGG AGTGAACTTG TTAGTGGGCA CTGAAAGTGG TCTGATGCTG 
    CTAGACAGAA GTGGTCAGGG GAAGGTTTAT CCGCTCATCA ATCGAAGACG ATTCCAGCAA 
    ATGGATGTAC TTGAAGGGCT GAATGTCTTG GTGACAATTT CTGGTAAGAA GAACAAGTTG 
    CGAGTTTACT ATTTGTCCTG GTTAAGAAAT AAGATCCTTC ACAATGACCC AGAAGTAGAA 
    AAGAAACAGG GCTGGACAAC GGTGGGAGAC TTGGAAGGCT GTGTGCACTA TAAAGTTGTA 
    AAATATGAAA GAATCAAGTT CTTGGTAATT GCATTGAAGA GTTCTGTGGA AGTCTATGCA 
    TGGGCACCAA AGCCATACCA TAAATTTATG GCCTTTAAGT CGTTTGGAGA ACTGGTACAT 
    AAGCCATTGC TCGTGGATCT AACCGTAGAA GAGGGTCAGA GACTAAAAGT CATCTATGGC 
    TCCTGCGCTG GCTTCCACGC CGTCGATGTG GATTCAGGAT CGGTCTATGA TATTTATTTA 
    CCAACACATA TCCAGAGCAG TATCCAACCT CACGCAATCA TCATTCTCCC AAACACGGAT 
    GGGATGGAGC TACTGGTCTG CTACGAGGAT GAAGGAGTTT ACGTCAACAC GTATGGAAGG 
    ATCACCAAGG ATGTGGTTCT GCAGTGGGGA GAAATGCCAA CTTCAGTTGC ATACATCCGA 
    TCCAACCAGA TTATGGGCTG GGGAGAAAAA GCTATTGAGA TACGATCGGT GGAAACTGGA 
    CACTTGGACG GGGTGTTCAT GCACAAAAGG GCACAAAGAC TCAAGTTCTT ATGCGAACGC 
    AATGACAAGG TTTTCTTTGC CTCCGTGCGG TCAGGTGGAA GCAGTCAAGT CTATTTCATG 
    ACCCTTGGCA GGACTTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813240.1
    CDS123..3875
    Translation

    Target ORF information:

    RefSeq Version XM_014957754.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACC TGTGAGAACA ACGTCCCGAT CGCCAGTCCT GTCCCGCCGT 
    GATTCTCCCC TGCAGGGCAG TGGGCAGCAA AATAACCAAG CAGGTCAAAG AAACTCCACG 
    AGCAATATAG AGCCTCGTCT GCTGTGGGAG AGGGTGGAGA AGCTCGTACC AAGGCCAGGC 
    AGTGGCAGTT CTTCGGGTTC AAGCAACTCC GGCTCACAGC CTGGCTCCCA CCCCGGCTCT 
    CAGAGTGGGT CTGGAGAGCG GTTCCGGATG AGATCATCAT CCAAATCAGA AGGTTCGCCA 
    TCCCAGCGTC ATGAAAGTGC ACCTAAAAAG CCTGAAGAGA AAAAGGAAGT CTTCAGACCT 
    ATCAAGCCTG CTGGAGAAGT GGATTTAACT GCATTGGCAA AGGAATTGCG AGCGGTAGAG 
    GATGTGCGAC CTCCACATAA AGTAACGGAT TATTCGTCCT CAAGTGAGGA GTCAGGGACG 
    ACAGATGAGG AAGATGATGA TATGGAACAG GAAGGAGCAG ACGAATCTAC TTCTGGACCA 
    GATGATGTTC GAGCAGTGTC AACATTGAAC TTGAGCAATG GTGAAACAGA GTCAGTGAAA 
    ACCATGATTG TCCACGATGA TGTGGAGAGT GAACCTGCCT TGACTCCTTC TAAGGAGGGC 
    ACCTTAATTG TCCGACAGAG TACAGTTGAC AAAAAGCGTG CCAGCCATCA TGAGAGCAAT 
    GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
    ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
    ACACCCCAGG ACAAGCTAAC TACTAATGAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGTGGGA CAACTGTGAC TTCTGTGGTA GGCTTTTCTA GTGAAGCAAT GAGATCAGAG 
    GCAATAAGGC AAGACCCGAC GCGGAAAGGA TCAGTTGTCA ATGTGAATCC CACGAATACG 
    CGGCCACAGA GCGATACCCC AGAGATTCGC AAATACAAGA AGAGATTCAA TTCTGAGATA 
    CTCTGTGCTG CCTTATGGGG AGTGAACTTG TTAGTGGGCA CTGAAAGTGG TCTGATGCTG 
    CTAGACAGAA GTGGTCAGGG GAAGGTTTAT CCGCTCATCA ATCGAAGACG ATTCCAGCAA 
    ATGGATGTAC TTGAAGGGCT GAATGTCTTG GTGACAATTT CTGGTAAGAA GAACAAGTTG 
    CGAGTTTACT ATTTGTCCTG GTTAAGAAAT AAGATCCTTC ACAATGACCC AGAAGTAGAA 
    AAGAAACAGG GCTGGACAAC GGTGGGAGAC TTGGAAGGCT GTGTGCACTA TAAAGTTGTA 
    AAATATGAAA GAATCAAGTT CTTGGTAATT GCATTGAAGA GTTCTGTGGA AGTCTATGCA 
    TGGGCACCAA AGCCATACCA TAAATTTATG GCCTTTAAGT CGTTTGGAGA ACTGGTACAT 
    AAGCCATTGC TCGTGGATCT AACCGTAGAA GAGGGTCAGA GACTAAAAGT CATCTATGGC 
    TCCTGCGCTG GCTTCCACGC CGTCGATGTG GATTCAGGAT CGGTCTATGA TATTTATTTA 
    CCAACACATA TCCAGAGCAG TATCCAACCT CACGCAATCA TCATTCTCCC AAACACGGAT 
    GGGATGGAGC TACTGGTCTG CTACGAGGAT GAAGGAGTTT ACGTCAACAC GTATGGAAGG 
    ATCACCAAGG ATGTGGTTCT GCAGTGGGGA GAAATGCCAA CTTCAGTTGC ATACATCCGA 
    TCCAACCAGA TTATGGGCTG GGGAGAAAAA GCTATTGAGA TACGATCGGT GGAAACTGGA 
    CACTTGGACG GGGTGTTCAT GCACAAAAGG GCACAAAGAC TCAAGTTCTT ATGCGAACGC 
    AATGACAAGG TTTTCTTTGC CTCCGTGCGG TCAGGTGGAA GCAGTCAAGT CTATTTCATG 
    ACCCTTGGCA GGACTTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957755.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813241.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OCa76586
    Clone ID Related Accession (Same CDS sequence) XM_014957755.1
    Accession Version XM_014957755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACC TGTGAGAACA ACGTCCCGAT CGCCAGTCCT GTCCCGCCGT 
    GATTCTCCCC TGCAGGGCAG TGGGCAGCAA AATAACCAAG CAGGTCAAAG AAACTCCACG 
    AGCAATATAG AGCCTCGTCT GCTGTGGGAG AGGGTGGAGA AGCTCGTACC AAGGCCAGGC 
    AGTGGCAGTT CTTCGGGTTC AAGCAACTCC GGCTCACAGC CTGGCTCCCA CCCCGGCTCT 
    CAGAGTGGGT CTGGAGAGCG GTTCCGGATG AGATCATCAT CCAAATCAGA AGGTTCGCCA 
    TCCCAGCGTC ATGAAAGTGC ACCTAAAAAG CCTGAAGAGA AAAAGGAAGT CTTCAGACCT 
    ATCAAGCCTG CTGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA GTACAGTTGA CAAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTAA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG 
    ACAACTGTGA CTTCTGTGGT AGGCTTTTCT AGTGAAGCAA TGAGATCAGA GGCAATAAGG 
    CAAGACCCGA CGCGGAAAGG ATCAGTTGTC AATGTGAATC CCACGAATAC GCGGCCACAG 
    AGCGATACCC CAGAGATTCG CAAATACAAG AAGAGATTCA ATTCTGAGAT ACTCTGTGCT 
    GCCTTATGGG GAGTGAACTT GTTAGTGGGC ACTGAAAGTG GTCTGATGCT GCTAGACAGA 
    AGTGGTCAGG GGAAGGTTTA TCCGCTCATC AATCGAAGAC GATTCCAGCA AATGGATGTA 
    CTTGAAGGGC TGAATGTCTT GGTGACAATT TCTGGTAAGA AGAACAAGTT GCGAGTTTAC 
    TATTTGTCCT GGTTAAGAAA TAAGATCCTT CACAATGACC CAGAAGTAGA AAAGAAACAG 
    GGCTGGACAA CGGTGGGAGA CTTGGAAGGC TGTGTGCACT ATAAAGTTGT AAAATATGAA 
    AGAATCAAGT TCTTGGTAAT TGCATTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCA 
    AAGCCATACC ATAAATTTAT GGCCTTTAAG TCGTTTGGAG AACTGGTACA TAAGCCATTG 
    CTCGTGGATC TAACCGTAGA AGAGGGTCAG AGACTAAAAG TCATCTATGG CTCCTGCGCT 
    GGCTTCCACG CCGTCGATGT GGATTCAGGA TCGGTCTATG ATATTTATTT ACCAACACAT 
    ATCCAGAGCA GTATCCAACC TCACGCAATC ATCATTCTCC CAAACACGGA TGGGATGGAG 
    CTACTGGTCT GCTACGAGGA TGAAGGAGTT TACGTCAACA CGTATGGAAG GATCACCAAG 
    GATGTGGTTC TGCAGTGGGG AGAAATGCCA ACTTCAGTTG CATACATCCG ATCCAACCAG 
    ATTATGGGCT GGGGAGAAAA AGCTATTGAG ATACGATCGG TGGAAACTGG ACACTTGGAC 
    GGGGTGTTCA TGCACAAAAG GGCACAAAGA CTCAAGTTCT TATGCGAACG CAATGACAAG 
    GTTTTCTTTG CCTCCGTGCG GTCAGGTGGA AGCAGTCAAG TCTATTTCAT GACCCTTGGC 
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813241.1
    CDS123..3866
    Translation

    Target ORF information:

    RefSeq Version XM_014957755.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACC TGTGAGAACA ACGTCCCGAT CGCCAGTCCT GTCCCGCCGT 
    GATTCTCCCC TGCAGGGCAG TGGGCAGCAA AATAACCAAG CAGGTCAAAG AAACTCCACG 
    AGCAATATAG AGCCTCGTCT GCTGTGGGAG AGGGTGGAGA AGCTCGTACC AAGGCCAGGC 
    AGTGGCAGTT CTTCGGGTTC AAGCAACTCC GGCTCACAGC CTGGCTCCCA CCCCGGCTCT 
    CAGAGTGGGT CTGGAGAGCG GTTCCGGATG AGATCATCAT CCAAATCAGA AGGTTCGCCA 
    TCCCAGCGTC ATGAAAGTGC ACCTAAAAAG CCTGAAGAGA AAAAGGAAGT CTTCAGACCT 
    ATCAAGCCTG CTGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA GTACAGTTGA CAAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTAA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG 
    ACAACTGTGA CTTCTGTGGT AGGCTTTTCT AGTGAAGCAA TGAGATCAGA GGCAATAAGG 
    CAAGACCCGA CGCGGAAAGG ATCAGTTGTC AATGTGAATC CCACGAATAC GCGGCCACAG 
    AGCGATACCC CAGAGATTCG CAAATACAAG AAGAGATTCA ATTCTGAGAT ACTCTGTGCT 
    GCCTTATGGG GAGTGAACTT GTTAGTGGGC ACTGAAAGTG GTCTGATGCT GCTAGACAGA 
    AGTGGTCAGG GGAAGGTTTA TCCGCTCATC AATCGAAGAC GATTCCAGCA AATGGATGTA 
    CTTGAAGGGC TGAATGTCTT GGTGACAATT TCTGGTAAGA AGAACAAGTT GCGAGTTTAC 
    TATTTGTCCT GGTTAAGAAA TAAGATCCTT CACAATGACC CAGAAGTAGA AAAGAAACAG 
    GGCTGGACAA CGGTGGGAGA CTTGGAAGGC TGTGTGCACT ATAAAGTTGT AAAATATGAA 
    AGAATCAAGT TCTTGGTAAT TGCATTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCA 
    AAGCCATACC ATAAATTTAT GGCCTTTAAG TCGTTTGGAG AACTGGTACA TAAGCCATTG 
    CTCGTGGATC TAACCGTAGA AGAGGGTCAG AGACTAAAAG TCATCTATGG CTCCTGCGCT 
    GGCTTCCACG CCGTCGATGT GGATTCAGGA TCGGTCTATG ATATTTATTT ACCAACACAT 
    ATCCAGAGCA GTATCCAACC TCACGCAATC ATCATTCTCC CAAACACGGA TGGGATGGAG 
    CTACTGGTCT GCTACGAGGA TGAAGGAGTT TACGTCAACA CGTATGGAAG GATCACCAAG 
    GATGTGGTTC TGCAGTGGGG AGAAATGCCA ACTTCAGTTG CATACATCCG ATCCAACCAG 
    ATTATGGGCT GGGGAGAAAA AGCTATTGAG ATACGATCGG TGGAAACTGG ACACTTGGAC 
    GGGGTGTTCA TGCACAAAAG GGCACAAAGA CTCAAGTTCT TATGCGAACG CAATGACAAG 
    GTTTTCTTTG CCTCCGTGCG GTCAGGTGGA AGCAGTCAAG TCTATTTCAT GACCCTTGGC 
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957756.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813242.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID OCa76587
    Clone ID Related Accession (Same CDS sequence) XM_014957756.1
    Accession Version XM_014957756.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACC TGTGAGAACA ACGTCCCGAT CGCCAGTCCT GTCCCGCCGT 
    GATTCTCCCC TGCAGGGCAG TGGGCAGCAA AATAACCAAG CAGGTCAAAG AAACTCCACG 
    AGCAATATAG AGCCTCGTCT GCTGTGGGAG AGGGTGGAGA AGCTCGTACC AAGGCCAGGC 
    AGTGGCAGTT CTTCGGGTTC AAGCAACTCC GGCTCACAGC CTGGCTCCCA CCCCGGCTCT 
    CAGAGTGGGT CTGGAGAGCG GTTCCGGATG AGATCATCAT CCAAATCAGA AGGTTCGCCA 
    TCCCAGCGTC ATGAAAGTGC ACCTAAAAAG CCTGAAGAGA AAAAGGAAGT CTTCAGACCT 
    ATCAAGCCTG CTGGAGAAGT GGATTTAACT GCATTGGCAA AGGAATTGCG AGCGGTAGAG 
    GATGTGCGAC CTCCACATAA AGTAACGGAT TATTCGTCCT CAAGTGAGGA GTCAGGGACG 
    ACAGATGAGG AAGATGATGA TATGGAACAG GAAGGAGCAG ACGAATCTAC TTCTGGACCA 
    GATGATGTTC GAGCAGTGTC AACATTGAAC TTGAGCAATG GTGAAACAGA GTCAGTGAAA 
    ACCATGATTG TCCACGATGA TGTGGAGAGT GAACCTGCCT TGACTCCTTC TAAGGAGGGC 
    ACCTTAATTG TCCGACAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC 
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA 
    ACTGTGACTT CTGTGGTAGG CTTTTCTAGT GAAGCAATGA GATCAGAGGC AATAAGGCAA 
    GACCCGACGC GGAAAGGATC AGTTGTCAAT GTGAATCCCA CGAATACGCG GCCACAGAGC 
    GATACCCCAG AGATTCGCAA ATACAAGAAG AGATTCAATT CTGAGATACT CTGTGCTGCC 
    TTATGGGGAG TGAACTTGTT AGTGGGCACT GAAAGTGGTC TGATGCTGCT AGACAGAAGT 
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGACGAT TCCAGCAAAT GGATGTACTT 
    GAAGGGCTGA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAT 
    TTGTCCTGGT TAAGAAATAA GATCCTTCAC AATGACCCAG AAGTAGAAAA GAAACAGGGC 
    TGGACAACGG TGGGAGACTT GGAAGGCTGT GTGCACTATA AAGTTGTAAA ATATGAAAGA 
    ATCAAGTTCT TGGTAATTGC ATTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCAAAG 
    CCATACCATA AATTTATGGC CTTTAAGTCG TTTGGAGAAC TGGTACATAA GCCATTGCTC 
    GTGGATCTAA CCGTAGAAGA GGGTCAGAGA CTAAAAGTCA TCTATGGCTC CTGCGCTGGC 
    TTCCACGCCG TCGATGTGGA TTCAGGATCG GTCTATGATA TTTATTTACC AACACATATC 
    CAGAGCAGTA TCCAACCTCA CGCAATCATC ATTCTCCCAA ACACGGATGG GATGGAGCTA 
    CTGGTCTGCT ACGAGGATGA AGGAGTTTAC GTCAACACGT ATGGAAGGAT CACCAAGGAT 
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ACATCCGATC CAACCAGATT 
    ATGGGCTGGG GAGAAAAAGC TATTGAGATA CGATCGGTGG AAACTGGACA CTTGGACGGG 
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GCGAACGCAA TGACAAGGTT 
    TTCTTTGCCT CCGTGCGGTC AGGTGGAAGC AGTCAAGTCT ATTTCATGAC CCTTGGCAGG 
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813242.1
    CDS123..3683
    Translation

    Target ORF information:

    RefSeq Version XM_014957756.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACC TGTGAGAACA ACGTCCCGAT CGCCAGTCCT GTCCCGCCGT 
    GATTCTCCCC TGCAGGGCAG TGGGCAGCAA AATAACCAAG CAGGTCAAAG AAACTCCACG 
    AGCAATATAG AGCCTCGTCT GCTGTGGGAG AGGGTGGAGA AGCTCGTACC AAGGCCAGGC 
    AGTGGCAGTT CTTCGGGTTC AAGCAACTCC GGCTCACAGC CTGGCTCCCA CCCCGGCTCT 
    CAGAGTGGGT CTGGAGAGCG GTTCCGGATG AGATCATCAT CCAAATCAGA AGGTTCGCCA 
    TCCCAGCGTC ATGAAAGTGC ACCTAAAAAG CCTGAAGAGA AAAAGGAAGT CTTCAGACCT 
    ATCAAGCCTG CTGGAGAAGT GGATTTAACT GCATTGGCAA AGGAATTGCG AGCGGTAGAG 
    GATGTGCGAC CTCCACATAA AGTAACGGAT TATTCGTCCT CAAGTGAGGA GTCAGGGACG 
    ACAGATGAGG AAGATGATGA TATGGAACAG GAAGGAGCAG ACGAATCTAC TTCTGGACCA 
    GATGATGTTC GAGCAGTGTC AACATTGAAC TTGAGCAATG GTGAAACAGA GTCAGTGAAA 
    ACCATGATTG TCCACGATGA TGTGGAGAGT GAACCTGCCT TGACTCCTTC TAAGGAGGGC 
    ACCTTAATTG TCCGACAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC 
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA 
    ACTGTGACTT CTGTGGTAGG CTTTTCTAGT GAAGCAATGA GATCAGAGGC AATAAGGCAA 
    GACCCGACGC GGAAAGGATC AGTTGTCAAT GTGAATCCCA CGAATACGCG GCCACAGAGC 
    GATACCCCAG AGATTCGCAA ATACAAGAAG AGATTCAATT CTGAGATACT CTGTGCTGCC 
    TTATGGGGAG TGAACTTGTT AGTGGGCACT GAAAGTGGTC TGATGCTGCT AGACAGAAGT 
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGACGAT TCCAGCAAAT GGATGTACTT 
    GAAGGGCTGA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAT 
    TTGTCCTGGT TAAGAAATAA GATCCTTCAC AATGACCCAG AAGTAGAAAA GAAACAGGGC 
    TGGACAACGG TGGGAGACTT GGAAGGCTGT GTGCACTATA AAGTTGTAAA ATATGAAAGA 
    ATCAAGTTCT TGGTAATTGC ATTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCAAAG 
    CCATACCATA AATTTATGGC CTTTAAGTCG TTTGGAGAAC TGGTACATAA GCCATTGCTC 
    GTGGATCTAA CCGTAGAAGA GGGTCAGAGA CTAAAAGTCA TCTATGGCTC CTGCGCTGGC 
    TTCCACGCCG TCGATGTGGA TTCAGGATCG GTCTATGATA TTTATTTACC AACACATATC 
    CAGAGCAGTA TCCAACCTCA CGCAATCATC ATTCTCCCAA ACACGGATGG GATGGAGCTA 
    CTGGTCTGCT ACGAGGATGA AGGAGTTTAC GTCAACACGT ATGGAAGGAT CACCAAGGAT 
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ACATCCGATC CAACCAGATT 
    ATGGGCTGGG GAGAAAAAGC TATTGAGATA CGATCGGTGG AAACTGGACA CTTGGACGGG 
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GCGAACGCAA TGACAAGGTT 
    TTCTTTGCCT CCGTGCGGTC AGGTGGAAGC AGTCAAGTCT ATTTCATGAC CCTTGGCAGG 
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_014957757.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813243.1 Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID OCa76588
    Clone ID Related Accession (Same CDS sequence) XM_014957757.1
    Accession Version XM_014957757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACC TGTGAGAACA ACGTCCCGAT CGCCAGTCCT GTCCCGCCGT 
    GATTCTCCCC TGCAGGGCAG TGGGCAGCAA AATAACCAAG CAGGTCAAAG AAACTCCACG 
    AGCAATATAG AGCCTCGTCT GCTGTGGGAG AGGGTGGAGA AGCTCGTACC AAGGCCAGGC 
    AGTGGCAGTT CTTCGGGTTC AAGCAACTCC GGCTCACAGC CTGGCTCCCA CCCCGGCTCT 
    CAGAGTGGGT CTGGAGAGCG GTTCCGGATG AGATCATCAT CCAAATCAGA AGGTTCGCCA 
    TCCCAGCGTC ATGAAAGTGC ACCTAAAAAG CCTGAAGAGA AAAAGGAAGT CTTCAGACCT 
    ATCAAGCCTG CTGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACTGTGACT 
    TCTGTGGTAG GCTTTTCTAG TGAAGCAATG AGATCAGAGG CAATAAGGCA AGACCCGACG 
    CGGAAAGGAT CAGTTGTCAA TGTGAATCCC ACGAATACGC GGCCACAGAG CGATACCCCA 
    GAGATTCGCA AATACAAGAA GAGATTCAAT TCTGAGATAC TCTGTGCTGC CTTATGGGGA 
    GTGAACTTGT TAGTGGGCAC TGAAAGTGGT CTGATGCTGC TAGACAGAAG TGGTCAGGGG 
    AAGGTTTATC CGCTCATCAA TCGAAGACGA TTCCAGCAAA TGGATGTACT TGAAGGGCTG 
    AATGTCTTGG TGACAATTTC TGGTAAGAAG AACAAGTTGC GAGTTTACTA TTTGTCCTGG 
    TTAAGAAATA AGATCCTTCA CAATGACCCA GAAGTAGAAA AGAAACAGGG CTGGACAACG 
    GTGGGAGACT TGGAAGGCTG TGTGCACTAT AAAGTTGTAA AATATGAAAG AATCAAGTTC 
    TTGGTAATTG CATTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCACCAAA GCCATACCAT 
    AAATTTATGG CCTTTAAGTC GTTTGGAGAA CTGGTACATA AGCCATTGCT CGTGGATCTA 
    ACCGTAGAAG AGGGTCAGAG ACTAAAAGTC ATCTATGGCT CCTGCGCTGG CTTCCACGCC 
    GTCGATGTGG ATTCAGGATC GGTCTATGAT ATTTATTTAC CAACACATAT CCAGAGCAGT 
    ATCCAACCTC ACGCAATCAT CATTCTCCCA AACACGGATG GGATGGAGCT ACTGGTCTGC 
    TACGAGGATG AAGGAGTTTA CGTCAACACG TATGGAAGGA TCACCAAGGA TGTGGTTCTG 
    CAGTGGGGAG AAATGCCAAC TTCAGTTGCA TACATCCGAT CCAACCAGAT TATGGGCTGG 
    GGAGAAAAAG CTATTGAGAT ACGATCGGTG GAAACTGGAC ACTTGGACGG GGTGTTCATG 
    CACAAAAGGG CACAAAGACT CAAGTTCTTA TGCGAACGCA ATGACAAGGT TTTCTTTGCC 
    TCCGTGCGGT CAGGTGGAAG CAGTCAAGTC TATTTCATGA CCCTTGGCAG GACTTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813243.1
    CDS123..3674
    Translation

    Target ORF information:

    RefSeq Version XM_014957757.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014957757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGTTA CCGAGGACGA AGAAGAAGAA ATCAAACTGG AGATTAATAT GCTAAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACTTAT TATGGTGCTT TCATTAAGAA AAGTCCTCCA 
    GGACATGACG ACCAACTGTG GCTTGTTATG GAGTTTTGTG GAGCAGGCTC TATCACAGAC 
    CTTGTGAAGA ACACAAAAGG GAATACTCTG AAAGAAGACT GGATAGCATA TATCTCCAGA 
    GAGATTCTCC GGGGGTTGGC ACATCTTCAT GCCCATCATG TGATTCACAG GGATATCAAA 
    GGGCAAAATG TGTTGTTAAC AGAGAACGCA GAAGTGAAAC TCGTTGATTT CGGTGTGAGC 
    GCTCAGCTGG ACAGGACAGT TGGCAGGAGA AACACTTTTA TTGGAACTCC GTACTGGATG 
    GCTCCAGAAG TTATTGCCTG TGATGAAAAT CCAGATGCTA CGTATGATTA CAGAAGTGAC 
    CTTTGGTCAT GCGGCATTAC AGCCATAGAG ATGGCAGAAG GAGCTCCCCC GCTTTGTGAC 
    ATGCATCCCA TGAGGGCACT CTTTCTGATC CCCAGGAACC CACCCCCACG GTTAAAATCC 
    AAGAAATGGT CAAAAAAGTT TTTCAGCTTT ATAGAAGGTT GTCTAGTGAA GAATTACATG 
    CAGCGACCTT CTACAGAGCA ACTCTTGAAA CATCCCTTTA TACGTGACCA ACCAAATGAA 
    AGGCAAGTCC GAATCCAGCT TAAGGACCAT ATAGACAGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAGA CAGAGTATGA ATATAGTGGA AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA 
    CAAGAAGGAG AGCCAAGTTC CATTGTCAAT GTGCCTGGAG AATCAACGCT CCGACGTGAT 
    TTCCTGAGGC TGCAGCAGGA AAACAAGGAA CGGTCCGAGG CCCTTCGGAG GCAGCAGCTG 
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTACAAGA GGCAGCTGCT GGCAGAGAGG 
    CAGAAGCGAA TCGAGCAGCA GAAGGAGCAA AGGAGACGGC TAGAAGAGCA ACAAAGAAGA 
    GAACGGGAAG CTCGAAGGCA ACAAGAGCGT GAGCAAAGGC GGAGAGAACA GGAAGAAAAG 
    AGACGTCTGG AAGAAATGGA GAGGAGGCGT AAGGAAGAGG AAGAGAGAAG AAGAGCAGAG 
    GAGGAAAAGA GGAGAGTAGA AAGGGAGCAG GAGTATATCA GGCGACAGCT AGAAGAGGAG 
    CAGCGGCACT TGGAAATTCT ACAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAA 
    TACAGATGGC GAGAGATGGA GGAACACCGA CAGGCGGAGC GACTCCAGCG CCAGTTGCAG 
    CAGGAGCAGG CCTACCTCCT GTCGCTGCAG CACGATCACA GGCGGCAGCA GCAGCAGCAG 
    CAGCAACAGC AGCAGCAGCA AGAAAGAAAT AAACAAAGCT ATCATACCCC TGAGCCAAAA 
    TCCCACTATG AGCCTGCTGA AAGAGCGCGT GAGGTGGAGG AAAGATTTAG AAAAACTAAT 
    CAGGGCTCCC CCGAAGCACA GTCTAAACAG ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT 
    TCAAGATCAG AGTCCTTTTC CAATGGAAAC TCAGAACCTG CTCAGCCTGC CCTGCAGAGG 
    CCGATGGAGC CCCAGGTACC TGTGAGAACA ACGTCCCGAT CGCCAGTCCT GTCCCGCCGT 
    GATTCTCCCC TGCAGGGCAG TGGGCAGCAA AATAACCAAG CAGGTCAAAG AAACTCCACG 
    AGCAATATAG AGCCTCGTCT GCTGTGGGAG AGGGTGGAGA AGCTCGTACC AAGGCCAGGC 
    AGTGGCAGTT CTTCGGGTTC AAGCAACTCC GGCTCACAGC CTGGCTCCCA CCCCGGCTCT 
    CAGAGTGGGT CTGGAGAGCG GTTCCGGATG AGATCATCAT CCAAATCAGA AGGTTCGCCA 
    TCCCAGCGTC ATGAAAGTGC ACCTAAAAAG CCTGAAGAGA AAAAGGAAGT CTTCAGACCT 
    ATCAAGCCTG CTGATTTAAC TGCATTGGCA AAGGAATTGC GAGCGGTAGA GGATGTGCGA 
    CCTCCACATA AAGTAACGGA TTATTCGTCC TCAAGTGAGG AGTCAGGGAC GACAGATGAG 
    GAAGATGATG ATATGGAACA GGAAGGAGCA GACGAATCTA CTTCTGGACC AGATGATGTT 
    CGAGCAGTGT CAACATTGAA CTTGAGCAAT GGTGAAACAG AGTCAGTGAA AACCATGATT 
    GTCCACGATG ATGTGGAGAG TGAACCTGCC TTGACTCCTT CTAAGGAGGG CACCTTAATT 
    GTCCGACAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACTGTGACT 
    TCTGTGGTAG GCTTTTCTAG TGAAGCAATG AGATCAGAGG CAATAAGGCA AGACCCGACG 
    CGGAAAGGAT CAGTTGTCAA TGTGAATCCC ACGAATACGC GGCCACAGAG CGATACCCCA 
    GAGATTCGCA AATACAAGAA GAGATTCAAT TCTGAGATAC TCTGTGCTGC CTTATGGGGA 
    GTGAACTTGT TAGTGGGCAC TGAAAGTGGT CTGATGCTGC TAGACAGAAG TGGTCAGGGG 
    AAGGTTTATC CGCTCATCAA TCGAAGACGA TTCCAGCAAA TGGATGTACT TGAAGGGCTG 
    AATGTCTTGG TGACAATTTC TGGTAAGAAG AACAAGTTGC GAGTTTACTA TTTGTCCTGG 
    TTAAGAAATA AGATCCTTCA CAATGACCCA GAAGTAGAAA AGAAACAGGG CTGGACAACG 
    GTGGGAGACT TGGAAGGCTG TGTGCACTAT AAAGTTGTAA AATATGAAAG AATCAAGTTC 
    TTGGTAATTG CATTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCACCAAA GCCATACCAT 
    AAATTTATGG CCTTTAAGTC GTTTGGAGAA CTGGTACATA AGCCATTGCT CGTGGATCTA 
    ACCGTAGAAG AGGGTCAGAG ACTAAAAGTC ATCTATGGCT CCTGCGCTGG CTTCCACGCC 
    GTCGATGTGG ATTCAGGATC GGTCTATGAT ATTTATTTAC CAACACATAT CCAGAGCAGT 
    ATCCAACCTC ACGCAATCAT CATTCTCCCA AACACGGATG GGATGGAGCT ACTGGTCTGC 
    TACGAGGATG AAGGAGTTTA CGTCAACACG TATGGAAGGA TCACCAAGGA TGTGGTTCTG 
    CAGTGGGGAG AAATGCCAAC TTCAGTTGCA TACATCCGAT CCAACCAGAT TATGGGCTGG 
    GGAGAAAAAG CTATTGAGAT ACGATCGGTG GAAACTGGAC ACTTGGACGG GGTGTTCATG 
    CACAAAAGGG CACAAAGACT CAAGTTCTTA TGCGAACGCA ATGACAAGGT TTTCTTTGCC 
    TCCGTGCGGT CAGGTGGAAG CAGTCAAGTC TATTTCATGA CCCTTGGCAG GACTTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

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