SEMA6A cDNA ORF clone, Calidris pugnax(ruff)

The following SEMA6A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA6A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa76673 XM_014957880.1
Latest version!
Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCa76671 XM_014957876.1
Latest version!
Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa76671 XM_014957877.1
Latest version!
Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa76671 XM_014957878.1
Latest version!
Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa76672 XM_014957879.1
Latest version!
Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa76673
    Clone ID Related Accession (Same CDS sequence) XM_014957880.1
    Accession Version XM_014957880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product semaphorin-6A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091091.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGG AGTGATTCGT
    GAGAGTTACC TGAAGGGTCA TGATCAGCTG GTGCCGGTCA CCCTGTTGGC CATTGCTGTT
    ATTCTTGCTT TTGTGATGGG GGCCATCTTT TCGGGAATCA TAGTGTACTG CATCTGTGAC
    CATCGCCGCA GAGATGTGGC TGTTTCTCAG AGGAAGGAGA AGGAGCTGAC CCACTCCCGC
    CGTGGCTCCA TGAGCAGCGT TACCAAGCTC AGTGGCCTCT TTGGGGACGC TCAGTCCAAG
    GAGCCAAAGC CTGAAGCTAT CCTCACGCCG CTGATGCACA ATGGCAAACT GGCTACCCCA
    GGCAGCACTG CCAAGATGCT AATCAAAGCT GACCAGCACC ATCTGGACCT GGCCGCTCTG
    CCAACCCCTG AGTCCACCCC AACCCTGCAA CAGAAGAGAA AGCCCAGCCG TGGCAGCAGA
    GAATGGGAGA GAAACCAGAA TCTCATCAAT GCCTGCACAA AAGATATCCC CCCGATGGCC
    TCGCCCGTTA TCCCGACTGA CCTGCCACTC AGGGCTTCTC CTAGCCACAT TCCCAGTGTT
    GTGGTCCTGC CCATAGCCCA GCAAGGCTAC CAGCATGAGT ACGTTGACCA GCCAAAAATG
    AGCGATGGGG CTCAGATGTC TGTGGAGGAC CAGAACGCCA CCCTTGAGTA CAAAACTATC
    AAGGACCATC TCAGTAGCAA AAGCCCCAGC CACGGGGTTA ACCTGGTGGA AAATCTCGAC
    AGCATGCCAC CAAAAGTACC CCAGCGGGAA GCTTCCCTCG GGCCCCCGAG CGCCTCGCTG
    TCGCAGAGCG GCCTGAGCAA GCGGCTGGAG ATGCACTCTT CTGCTTACAA CGTGGACTAC
    AAGAGAAGCT ACCCTACAAA CTCACTCACA AGAAGTCACC AGACGTCGAC CCTCAAGAGA
    AACAACACCA ACTCCTCTAA CTCGTCCCAC CTCTCCCGCA ATCAGAGCTT CGGCCGGGGC
    GACAACCCGC CACCGGCCCC GCAGCGAGTA GACTCAATAC AAGTCCACGC TGCTCAAGCA
    CAGGCTGTGA CTGTATCTCG GCAGCCAAGT CTCAGCGCCT ACAACTCGCT GACGAGGTCA
    GGGTTGAAAC GCACACCCTC GCTAAAGCCA GACGTACCTC CCAAACCTTC CTTTGCGCCG
    CTCTCCACGT CCATGAAGCC CAACGACGCA TGTACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813366.1
    CDS312..3230
    Translation

    Target ORF information:

    RefSeq Version XM_014957880.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014957880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGG AGTGATTCGT
    GAGAGTTACC TGAAGGGTCA TGATCAGCTG GTGCCGGTCA CCCTGTTGGC CATTGCTGTT
    ATTCTTGCTT TTGTGATGGG GGCCATCTTT TCGGGAATCA TAGTGTACTG CATCTGTGAC
    CATCGCCGCA GAGATGTGGC TGTTTCTCAG AGGAAGGAGA AGGAGCTGAC CCACTCCCGC
    CGTGGCTCCA TGAGCAGCGT TACCAAGCTC AGTGGCCTCT TTGGGGACGC TCAGTCCAAG
    GAGCCAAAGC CTGAAGCTAT CCTCACGCCG CTGATGCACA ATGGCAAACT GGCTACCCCA
    GGCAGCACTG CCAAGATGCT AATCAAAGCT GACCAGCACC ATCTGGACCT GGCCGCTCTG
    CCAACCCCTG AGTCCACCCC AACCCTGCAA CAGAAGAGAA AGCCCAGCCG TGGCAGCAGA
    GAATGGGAGA GAAACCAGAA TCTCATCAAT GCCTGCACAA AAGATATCCC CCCGATGGCC
    TCGCCCGTTA TCCCGACTGA CCTGCCACTC AGGGCTTCTC CTAGCCACAT TCCCAGTGTT
    GTGGTCCTGC CCATAGCCCA GCAAGGCTAC CAGCATGAGT ACGTTGACCA GCCAAAAATG
    AGCGATGGGG CTCAGATGTC TGTGGAGGAC CAGAACGCCA CCCTTGAGTA CAAAACTATC
    AAGGACCATC TCAGTAGCAA AAGCCCCAGC CACGGGGTTA ACCTGGTGGA AAATCTCGAC
    AGCATGCCAC CAAAAGTACC CCAGCGGGAA GCTTCCCTCG GGCCCCCGAG CGCCTCGCTG
    TCGCAGAGCG GCCTGAGCAA GCGGCTGGAG ATGCACTCTT CTGCTTACAA CGTGGACTAC
    AAGAGAAGCT ACCCTACAAA CTCACTCACA AGAAGTCACC AGACGTCGAC CCTCAAGAGA
    AACAACACCA ACTCCTCTAA CTCGTCCCAC CTCTCCCGCA ATCAGAGCTT CGGCCGGGGC
    GACAACCCGC CACCGGCCCC GCAGCGAGTA GACTCAATAC AAGTCCACGC TGCTCAAGCA
    CAGGCTGTGA CTGTATCTCG GCAGCCAAGT CTCAGCGCCT ACAACTCGCT GACGAGGTCA
    GGGTTGAAAC GCACACCCTC GCTAAAGCCA GACGTACCTC CCAAACCTTC CTTTGCGCCG
    CTCTCCACGT CCATGAAGCC CAACGACGCA TGTACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa76671
    Clone ID Related Accession (Same CDS sequence) XM_014957876.1 , XM_014957878.1 , XM_014957877.1
    Accession Version XM_014957876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product semaphorin-6A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091091.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGA CATTTCAACT
    GCTTCACCTG ATCATGAAAT GTCTTACGAC ACAGTGTATG GACACTCCAG TTCCCTAGTC
    CCAAGCACCA CCACTTCCGA CTCTCGCGCT CGACAGGGTT ATGATGCTAG GGGACGCATG
    CTGGATTGGA AGGATCCGCT GGGTTCATCT GAAAATACAG ATCCATATGT GGCAGTTTCA
    TCCCATAATC ATCAAGACAA GAAGGGAGTG ATTCGTGAGA GTTACCTGAA GGGTCATGAT
    CAGCTGGTGC CGGTCACCCT GTTGGCCATT GCTGTTATTC TTGCTTTTGT GATGGGGGCC
    ATCTTTTCGG GAATCATAGT GTACTGCATC TGTGACCATC GCCGCAGAGA TGTGGCTGTT
    TCTCAGAGGA AGGAGAAGGA GCTGACCCAC TCCCGCCGTG GCTCCATGAG CAGCGTTACC
    AAGCTCAGTG GCCTCTTTGG GGACGCTCAG TCCAAGGAGC CAAAGCCTGA AGCTATCCTC
    ACGCCGCTGA TGCACAATGG CAAACTGGCT ACCCCAGGCA GCACTGCCAA GATGCTAATC
    AAAGCTGACC AGCACCATCT GGACCTGGCC GCTCTGCCAA CCCCTGAGTC CACCCCAACC
    CTGCAACAGA AGAGAAAGCC CAGCCGTGGC AGCAGAGAAT GGGAGAGAAA CCAGAATCTC
    ATCAATGCCT GCACAAAAGA TATCCCCCCG ATGGCCTCGC CCGTTATCCC GACTGACCTG
    CCACTCAGGG CTTCTCCTAG CCACATTCCC AGTGTTGTGG TCCTGCCCAT AGCCCAGCAA
    GGCTACCAGC ATGAGTACGT TGACCAGCCA AAAATGAGCG ATGGGGCTCA GATGTCTGTG
    GAGGACCAGA ACGCCACCCT TGAGTACAAA ACTATCAAGG ACCATCTCAG TAGCAAAAGC
    CCCAGCCACG GGGTTAACCT GGTGGAAAAT CTCGACAGCA TGCCACCAAA AGTACCCCAG
    CGGGAAGCTT CCCTCGGGCC CCCGAGCGCC TCGCTGTCGC AGAGCGGCCT GAGCAAGCGG
    CTGGAGATGC ACTCTTCTGC TTACAACGTG GACTACAAGA GAAGCTACCC TACAAACTCA
    CTCACAAGAA GTCACCAGAC GTCGACCCTC AAGAGAAACA ACACCAACTC CTCTAACTCG
    TCCCACCTCT CCCGCAATCA GAGCTTCGGC CGGGGCGACA ACCCGCCACC GGCCCCGCAG
    CGAGTAGACT CAATACAAGT CCACGCTGCT CAAGCACAGG CTGTGACTGT ATCTCGGCAG
    CCAAGTCTCA GCGCCTACAA CTCGCTGACG AGGTCAGGGT TGAAACGCAC ACCCTCGCTA
    AAGCCAGACG TACCTCCCAA ACCTTCCTTT GCGCCGCTCT CCACGTCCAT GAAGCCCAAC
    GACGCATGTA CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813362.1
    CDS312..3446
    Translation

    Target ORF information:

    RefSeq Version XM_014957876.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014957876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGA CATTTCAACT
    GCTTCACCTG ATCATGAAAT GTCTTACGAC ACAGTGTATG GACACTCCAG TTCCCTAGTC
    CCAAGCACCA CCACTTCCGA CTCTCGCGCT CGACAGGGTT ATGATGCTAG GGGACGCATG
    CTGGATTGGA AGGATCCGCT GGGTTCATCT GAAAATACAG ATCCATATGT GGCAGTTTCA
    TCCCATAATC ATCAAGACAA GAAGGGAGTG ATTCGTGAGA GTTACCTGAA GGGTCATGAT
    CAGCTGGTGC CGGTCACCCT GTTGGCCATT GCTGTTATTC TTGCTTTTGT GATGGGGGCC
    ATCTTTTCGG GAATCATAGT GTACTGCATC TGTGACCATC GCCGCAGAGA TGTGGCTGTT
    TCTCAGAGGA AGGAGAAGGA GCTGACCCAC TCCCGCCGTG GCTCCATGAG CAGCGTTACC
    AAGCTCAGTG GCCTCTTTGG GGACGCTCAG TCCAAGGAGC CAAAGCCTGA AGCTATCCTC
    ACGCCGCTGA TGCACAATGG CAAACTGGCT ACCCCAGGCA GCACTGCCAA GATGCTAATC
    AAAGCTGACC AGCACCATCT GGACCTGGCC GCTCTGCCAA CCCCTGAGTC CACCCCAACC
    CTGCAACAGA AGAGAAAGCC CAGCCGTGGC AGCAGAGAAT GGGAGAGAAA CCAGAATCTC
    ATCAATGCCT GCACAAAAGA TATCCCCCCG ATGGCCTCGC CCGTTATCCC GACTGACCTG
    CCACTCAGGG CTTCTCCTAG CCACATTCCC AGTGTTGTGG TCCTGCCCAT AGCCCAGCAA
    GGCTACCAGC ATGAGTACGT TGACCAGCCA AAAATGAGCG ATGGGGCTCA GATGTCTGTG
    GAGGACCAGA ACGCCACCCT TGAGTACAAA ACTATCAAGG ACCATCTCAG TAGCAAAAGC
    CCCAGCCACG GGGTTAACCT GGTGGAAAAT CTCGACAGCA TGCCACCAAA AGTACCCCAG
    CGGGAAGCTT CCCTCGGGCC CCCGAGCGCC TCGCTGTCGC AGAGCGGCCT GAGCAAGCGG
    CTGGAGATGC ACTCTTCTGC TTACAACGTG GACTACAAGA GAAGCTACCC TACAAACTCA
    CTCACAAGAA GTCACCAGAC GTCGACCCTC AAGAGAAACA ACACCAACTC CTCTAACTCG
    TCCCACCTCT CCCGCAATCA GAGCTTCGGC CGGGGCGACA ACCCGCCACC GGCCCCGCAG
    CGAGTAGACT CAATACAAGT CCACGCTGCT CAAGCACAGG CTGTGACTGT ATCTCGGCAG
    CCAAGTCTCA GCGCCTACAA CTCGCTGACG AGGTCAGGGT TGAAACGCAC ACCCTCGCTA
    AAGCCAGACG TACCTCCCAA ACCTTCCTTT GCGCCGCTCT CCACGTCCAT GAAGCCCAAC
    GACGCATGTA CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa76671
    Clone ID Related Accession (Same CDS sequence) XM_014957876.1 , XM_014957878.1 , XM_014957877.1
    Accession Version XM_014957878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product semaphorin-6A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091091.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGA CATTTCAACT
    GCTTCACCTG ATCATGAAAT GTCTTACGAC ACAGTGTATG GACACTCCAG TTCCCTAGTC
    CCAAGCACCA CCACTTCCGA CTCTCGCGCT CGACAGGGTT ATGATGCTAG GGGACGCATG
    CTGGATTGGA AGGATCCGCT GGGTTCATCT GAAAATACAG ATCCATATGT GGCAGTTTCA
    TCCCATAATC ATCAAGACAA GAAGGGAGTG ATTCGTGAGA GTTACCTGAA GGGTCATGAT
    CAGCTGGTGC CGGTCACCCT GTTGGCCATT GCTGTTATTC TTGCTTTTGT GATGGGGGCC
    ATCTTTTCGG GAATCATAGT GTACTGCATC TGTGACCATC GCCGCAGAGA TGTGGCTGTT
    TCTCAGAGGA AGGAGAAGGA GCTGACCCAC TCCCGCCGTG GCTCCATGAG CAGCGTTACC
    AAGCTCAGTG GCCTCTTTGG GGACGCTCAG TCCAAGGAGC CAAAGCCTGA AGCTATCCTC
    ACGCCGCTGA TGCACAATGG CAAACTGGCT ACCCCAGGCA GCACTGCCAA GATGCTAATC
    AAAGCTGACC AGCACCATCT GGACCTGGCC GCTCTGCCAA CCCCTGAGTC CACCCCAACC
    CTGCAACAGA AGAGAAAGCC CAGCCGTGGC AGCAGAGAAT GGGAGAGAAA CCAGAATCTC
    ATCAATGCCT GCACAAAAGA TATCCCCCCG ATGGCCTCGC CCGTTATCCC GACTGACCTG
    CCACTCAGGG CTTCTCCTAG CCACATTCCC AGTGTTGTGG TCCTGCCCAT AGCCCAGCAA
    GGCTACCAGC ATGAGTACGT TGACCAGCCA AAAATGAGCG ATGGGGCTCA GATGTCTGTG
    GAGGACCAGA ACGCCACCCT TGAGTACAAA ACTATCAAGG ACCATCTCAG TAGCAAAAGC
    CCCAGCCACG GGGTTAACCT GGTGGAAAAT CTCGACAGCA TGCCACCAAA AGTACCCCAG
    CGGGAAGCTT CCCTCGGGCC CCCGAGCGCC TCGCTGTCGC AGAGCGGCCT GAGCAAGCGG
    CTGGAGATGC ACTCTTCTGC TTACAACGTG GACTACAAGA GAAGCTACCC TACAAACTCA
    CTCACAAGAA GTCACCAGAC GTCGACCCTC AAGAGAAACA ACACCAACTC CTCTAACTCG
    TCCCACCTCT CCCGCAATCA GAGCTTCGGC CGGGGCGACA ACCCGCCACC GGCCCCGCAG
    CGAGTAGACT CAATACAAGT CCACGCTGCT CAAGCACAGG CTGTGACTGT ATCTCGGCAG
    CCAAGTCTCA GCGCCTACAA CTCGCTGACG AGGTCAGGGT TGAAACGCAC ACCCTCGCTA
    AAGCCAGACG TACCTCCCAA ACCTTCCTTT GCGCCGCTCT CCACGTCCAT GAAGCCCAAC
    GACGCATGTA CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813364.1
    CDS71..3205
    Translation

    Target ORF information:

    RefSeq Version XM_014957878.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014957878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGA CATTTCAACT
    GCTTCACCTG ATCATGAAAT GTCTTACGAC ACAGTGTATG GACACTCCAG TTCCCTAGTC
    CCAAGCACCA CCACTTCCGA CTCTCGCGCT CGACAGGGTT ATGATGCTAG GGGACGCATG
    CTGGATTGGA AGGATCCGCT GGGTTCATCT GAAAATACAG ATCCATATGT GGCAGTTTCA
    TCCCATAATC ATCAAGACAA GAAGGGAGTG ATTCGTGAGA GTTACCTGAA GGGTCATGAT
    CAGCTGGTGC CGGTCACCCT GTTGGCCATT GCTGTTATTC TTGCTTTTGT GATGGGGGCC
    ATCTTTTCGG GAATCATAGT GTACTGCATC TGTGACCATC GCCGCAGAGA TGTGGCTGTT
    TCTCAGAGGA AGGAGAAGGA GCTGACCCAC TCCCGCCGTG GCTCCATGAG CAGCGTTACC
    AAGCTCAGTG GCCTCTTTGG GGACGCTCAG TCCAAGGAGC CAAAGCCTGA AGCTATCCTC
    ACGCCGCTGA TGCACAATGG CAAACTGGCT ACCCCAGGCA GCACTGCCAA GATGCTAATC
    AAAGCTGACC AGCACCATCT GGACCTGGCC GCTCTGCCAA CCCCTGAGTC CACCCCAACC
    CTGCAACAGA AGAGAAAGCC CAGCCGTGGC AGCAGAGAAT GGGAGAGAAA CCAGAATCTC
    ATCAATGCCT GCACAAAAGA TATCCCCCCG ATGGCCTCGC CCGTTATCCC GACTGACCTG
    CCACTCAGGG CTTCTCCTAG CCACATTCCC AGTGTTGTGG TCCTGCCCAT AGCCCAGCAA
    GGCTACCAGC ATGAGTACGT TGACCAGCCA AAAATGAGCG ATGGGGCTCA GATGTCTGTG
    GAGGACCAGA ACGCCACCCT TGAGTACAAA ACTATCAAGG ACCATCTCAG TAGCAAAAGC
    CCCAGCCACG GGGTTAACCT GGTGGAAAAT CTCGACAGCA TGCCACCAAA AGTACCCCAG
    CGGGAAGCTT CCCTCGGGCC CCCGAGCGCC TCGCTGTCGC AGAGCGGCCT GAGCAAGCGG
    CTGGAGATGC ACTCTTCTGC TTACAACGTG GACTACAAGA GAAGCTACCC TACAAACTCA
    CTCACAAGAA GTCACCAGAC GTCGACCCTC AAGAGAAACA ACACCAACTC CTCTAACTCG
    TCCCACCTCT CCCGCAATCA GAGCTTCGGC CGGGGCGACA ACCCGCCACC GGCCCCGCAG
    CGAGTAGACT CAATACAAGT CCACGCTGCT CAAGCACAGG CTGTGACTGT ATCTCGGCAG
    CCAAGTCTCA GCGCCTACAA CTCGCTGACG AGGTCAGGGT TGAAACGCAC ACCCTCGCTA
    AAGCCAGACG TACCTCCCAA ACCTTCCTTT GCGCCGCTCT CCACGTCCAT GAAGCCCAAC
    GACGCATGTA CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa76671
    Clone ID Related Accession (Same CDS sequence) XM_014957876.1 , XM_014957878.1 , XM_014957877.1
    Accession Version XM_014957877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product semaphorin-6A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091091.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGA CATTTCAACT
    GCTTCACCTG ATCATGAAAT GTCTTACGAC ACAGTGTATG GACACTCCAG TTCCCTAGTC
    CCAAGCACCA CCACTTCCGA CTCTCGCGCT CGACAGGGTT ATGATGCTAG GGGACGCATG
    CTGGATTGGA AGGATCCGCT GGGTTCATCT GAAAATACAG ATCCATATGT GGCAGTTTCA
    TCCCATAATC ATCAAGACAA GAAGGGAGTG ATTCGTGAGA GTTACCTGAA GGGTCATGAT
    CAGCTGGTGC CGGTCACCCT GTTGGCCATT GCTGTTATTC TTGCTTTTGT GATGGGGGCC
    ATCTTTTCGG GAATCATAGT GTACTGCATC TGTGACCATC GCCGCAGAGA TGTGGCTGTT
    TCTCAGAGGA AGGAGAAGGA GCTGACCCAC TCCCGCCGTG GCTCCATGAG CAGCGTTACC
    AAGCTCAGTG GCCTCTTTGG GGACGCTCAG TCCAAGGAGC CAAAGCCTGA AGCTATCCTC
    ACGCCGCTGA TGCACAATGG CAAACTGGCT ACCCCAGGCA GCACTGCCAA GATGCTAATC
    AAAGCTGACC AGCACCATCT GGACCTGGCC GCTCTGCCAA CCCCTGAGTC CACCCCAACC
    CTGCAACAGA AGAGAAAGCC CAGCCGTGGC AGCAGAGAAT GGGAGAGAAA CCAGAATCTC
    ATCAATGCCT GCACAAAAGA TATCCCCCCG ATGGCCTCGC CCGTTATCCC GACTGACCTG
    CCACTCAGGG CTTCTCCTAG CCACATTCCC AGTGTTGTGG TCCTGCCCAT AGCCCAGCAA
    GGCTACCAGC ATGAGTACGT TGACCAGCCA AAAATGAGCG ATGGGGCTCA GATGTCTGTG
    GAGGACCAGA ACGCCACCCT TGAGTACAAA ACTATCAAGG ACCATCTCAG TAGCAAAAGC
    CCCAGCCACG GGGTTAACCT GGTGGAAAAT CTCGACAGCA TGCCACCAAA AGTACCCCAG
    CGGGAAGCTT CCCTCGGGCC CCCGAGCGCC TCGCTGTCGC AGAGCGGCCT GAGCAAGCGG
    CTGGAGATGC ACTCTTCTGC TTACAACGTG GACTACAAGA GAAGCTACCC TACAAACTCA
    CTCACAAGAA GTCACCAGAC GTCGACCCTC AAGAGAAACA ACACCAACTC CTCTAACTCG
    TCCCACCTCT CCCGCAATCA GAGCTTCGGC CGGGGCGACA ACCCGCCACC GGCCCCGCAG
    CGAGTAGACT CAATACAAGT CCACGCTGCT CAAGCACAGG CTGTGACTGT ATCTCGGCAG
    CCAAGTCTCA GCGCCTACAA CTCGCTGACG AGGTCAGGGT TGAAACGCAC ACCCTCGCTA
    AAGCCAGACG TACCTCCCAA ACCTTCCTTT GCGCCGCTCT CCACGTCCAT GAAGCCCAAC
    GACGCATGTA CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813363.1
    CDS118..3252
    Translation

    Target ORF information:

    RefSeq Version XM_014957877.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014957877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGA CATTTCAACT
    GCTTCACCTG ATCATGAAAT GTCTTACGAC ACAGTGTATG GACACTCCAG TTCCCTAGTC
    CCAAGCACCA CCACTTCCGA CTCTCGCGCT CGACAGGGTT ATGATGCTAG GGGACGCATG
    CTGGATTGGA AGGATCCGCT GGGTTCATCT GAAAATACAG ATCCATATGT GGCAGTTTCA
    TCCCATAATC ATCAAGACAA GAAGGGAGTG ATTCGTGAGA GTTACCTGAA GGGTCATGAT
    CAGCTGGTGC CGGTCACCCT GTTGGCCATT GCTGTTATTC TTGCTTTTGT GATGGGGGCC
    ATCTTTTCGG GAATCATAGT GTACTGCATC TGTGACCATC GCCGCAGAGA TGTGGCTGTT
    TCTCAGAGGA AGGAGAAGGA GCTGACCCAC TCCCGCCGTG GCTCCATGAG CAGCGTTACC
    AAGCTCAGTG GCCTCTTTGG GGACGCTCAG TCCAAGGAGC CAAAGCCTGA AGCTATCCTC
    ACGCCGCTGA TGCACAATGG CAAACTGGCT ACCCCAGGCA GCACTGCCAA GATGCTAATC
    AAAGCTGACC AGCACCATCT GGACCTGGCC GCTCTGCCAA CCCCTGAGTC CACCCCAACC
    CTGCAACAGA AGAGAAAGCC CAGCCGTGGC AGCAGAGAAT GGGAGAGAAA CCAGAATCTC
    ATCAATGCCT GCACAAAAGA TATCCCCCCG ATGGCCTCGC CCGTTATCCC GACTGACCTG
    CCACTCAGGG CTTCTCCTAG CCACATTCCC AGTGTTGTGG TCCTGCCCAT AGCCCAGCAA
    GGCTACCAGC ATGAGTACGT TGACCAGCCA AAAATGAGCG ATGGGGCTCA GATGTCTGTG
    GAGGACCAGA ACGCCACCCT TGAGTACAAA ACTATCAAGG ACCATCTCAG TAGCAAAAGC
    CCCAGCCACG GGGTTAACCT GGTGGAAAAT CTCGACAGCA TGCCACCAAA AGTACCCCAG
    CGGGAAGCTT CCCTCGGGCC CCCGAGCGCC TCGCTGTCGC AGAGCGGCCT GAGCAAGCGG
    CTGGAGATGC ACTCTTCTGC TTACAACGTG GACTACAAGA GAAGCTACCC TACAAACTCA
    CTCACAAGAA GTCACCAGAC GTCGACCCTC AAGAGAAACA ACACCAACTC CTCTAACTCG
    TCCCACCTCT CCCGCAATCA GAGCTTCGGC CGGGGCGACA ACCCGCCACC GGCCCCGCAG
    CGAGTAGACT CAATACAAGT CCACGCTGCT CAAGCACAGG CTGTGACTGT ATCTCGGCAG
    CCAAGTCTCA GCGCCTACAA CTCGCTGACG AGGTCAGGGT TGAAACGCAC ACCCTCGCTA
    AAGCCAGACG TACCTCCCAA ACCTTCCTTT GCGCCGCTCT CCACGTCCAT GAAGCCCAAC
    GACGCATGTA CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa76672
    Clone ID Related Accession (Same CDS sequence) XM_014957879.1
    Accession Version XM_014957879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3084bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product semaphorin-6A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091091.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGG ACACTCCAGT
    TCCCTAGTCC CAAGCACCAC CACTTCCGAC TCTCGCGCTC GACAGGGTTA TGATGCTAGG
    GGACGCATGC TGGATTGGAA GGATCCGCTG GGTTCATCTG AAAATACAGA TCCATATGTG
    GCAGTTTCAT CCCATAATCA TCAAGACAAG AAGGGAGTGA TTCGTGAGAG TTACCTGAAG
    GGTCATGATC AGCTGGTGCC GGTCACCCTG TTGGCCATTG CTGTTATTCT TGCTTTTGTG
    ATGGGGGCCA TCTTTTCGGG AATCATAGTG TACTGCATCT GTGACCATCG CCGCAGAGAT
    GTGGCTGTTT CTCAGAGGAA GGAGAAGGAG CTGACCCACT CCCGCCGTGG CTCCATGAGC
    AGCGTTACCA AGCTCAGTGG CCTCTTTGGG GACGCTCAGT CCAAGGAGCC AAAGCCTGAA
    GCTATCCTCA CGCCGCTGAT GCACAATGGC AAACTGGCTA CCCCAGGCAG CACTGCCAAG
    ATGCTAATCA AAGCTGACCA GCACCATCTG GACCTGGCCG CTCTGCCAAC CCCTGAGTCC
    ACCCCAACCC TGCAACAGAA GAGAAAGCCC AGCCGTGGCA GCAGAGAATG GGAGAGAAAC
    CAGAATCTCA TCAATGCCTG CACAAAAGAT ATCCCCCCGA TGGCCTCGCC CGTTATCCCG
    ACTGACCTGC CACTCAGGGC TTCTCCTAGC CACATTCCCA GTGTTGTGGT CCTGCCCATA
    GCCCAGCAAG GCTACCAGCA TGAGTACGTT GACCAGCCAA AAATGAGCGA TGGGGCTCAG
    ATGTCTGTGG AGGACCAGAA CGCCACCCTT GAGTACAAAA CTATCAAGGA CCATCTCAGT
    AGCAAAAGCC CCAGCCACGG GGTTAACCTG GTGGAAAATC TCGACAGCAT GCCACCAAAA
    GTACCCCAGC GGGAAGCTTC CCTCGGGCCC CCGAGCGCCT CGCTGTCGCA GAGCGGCCTG
    AGCAAGCGGC TGGAGATGCA CTCTTCTGCT TACAACGTGG ACTACAAGAG AAGCTACCCT
    ACAAACTCAC TCACAAGAAG TCACCAGACG TCGACCCTCA AGAGAAACAA CACCAACTCC
    TCTAACTCGT CCCACCTCTC CCGCAATCAG AGCTTCGGCC GGGGCGACAA CCCGCCACCG
    GCCCCGCAGC GAGTAGACTC AATACAAGTC CACGCTGCTC AAGCACAGGC TGTGACTGTA
    TCTCGGCAGC CAAGTCTCAG CGCCTACAAC TCGCTGACGA GGTCAGGGTT GAAACGCACA
    CCCTCGCTAA AGCCAGACGT ACCTCCCAAA CCTTCCTTTG CGCCGCTCTC CACGTCCATG
    AAGCCCAACG ACGCATGTAC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014813365.1
    CDS312..3395
    Translation

    Target ORF information:

    RefSeq Version XM_014957879.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014957879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTCAG AAGCCTTGCT GCTATATTTC ACACTGCTAC ACTTTGCTGG GGCTGGTTTC 
    CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAGACA GTATCCGGTG
    TTCGTGGGTC ACAAACCAGG GCGGAACACC ACGCAGAGGC ACCGGCTGGA CATCCAGCTT
    GTCATGGTCA TGAACAGGAC CCTCTACGTT GCTGCTAGGG ACCATATTTA TACTGTTGAT
    ATGGACGCAT CACATACAGA AGAAATTTAT TTTAGCAAAA AATTGACGTG GAAATCTAGA
    CAAGCTGACG TAGACACATG CAGAATGAAG GGAAAACATA AGGATGAATG TCATAATTTT
    ATTAAGGTTC TCCTAAAAAG AAATGAGGAT ACATTGTTTA TCTGTGGAAC CAATGCATTC
    AACCCATCTT GCAGGAACTA CAAGATGGAT ACCCTGGAGT TCCTGGGAGA TGAATTCAGT
    GGAATGGCCA GATGCCCATA TGATGCAAAG CATGCCAACG TTGCGTTGTT CGCAGAGGGA
    AAACTGTATT CTGCCACAGT GACCGACTTC CTTGCAATAG ATGCAGTCAT ATACCGGAGC
    CTTGGAGACA GCCCAACTCT GCGGACAGTT AAACATGACT CAAAGTGGTT GAAAGAACCA
    TACTTTGTCC AGGCAGTGGA CTATGGCAAT TACATTTACT TCTTCTTCCG GGAAATAGCA
    GTGGAGTACA ACAGCATGGG AAAGGTAGTT TTCCCAAGGG TGGCTCAGGT TTGCAAAAAT
    GACATGGGAG GATCTCAGAG AGTGCTGGAA AAACAGTGGA CTTCCTTTCT CAAGGCTCGT
    TTGAACTGCT CAGTTCCTGG CGATTCACAC TTCTACTTTA ACATACTGCA GGCAGTTACA
    GATGTTATCC ATTTCAACGG CCGGGATGTT GTTTTAGCAA CCTTCTCCAC TCCATATAAT
    AGCATCCCTG GCTCTGCAGT CTGTGCCTAT GACATGCTTG ACATCGCCAA TGTATTTACT
    GGGAGATTCA AAGAACAAAA GTCTCCAGAC TCCACATGGA CACCTGTTCC TGATGAACGA
    GTGCCCAAGC CCAGGCCTGG TTGCTGTGCT GGCTCTACAT CATTAGAAAA ATATGCAACC
    TCCAACGAGT TCCCAGATGA TACTCTGAAC TTCATCAAAA CACATCCACT GATGGACGAG
    GCGGTACCGT CCATTGTCAA TCGACCCTGG TTCCTGAGGA CAATGGTCAG GTACCGCCTG
    ACCAAGATTG CTGTGGACTC TGCTGCTGGG CCATATCAGA ACTACACCGT GGTTTTCTTG
    GGATCAGAGA AGGGAATAAT CCTGAAATTC TTGGCACGGA CAGGAAACAG TGGTTTCCTA
    AATGACAGCC TTTTCCTGGA GGAGATGAAC GTTTACAATC CTGAAAAGTG CAGCTACGAT
    GGCGTGGAGG ACAAGCGCAT TGTGGGCATG CAGCTCGACA AGCCCAGCAG CGCCCTGTAT
    GTCGCCTTCT CCACCTGTGT CATCAAGGTT CCCCTGGGAC GTTGTGAGCG TCATGGCAAA
    TGCAAAAAGG CCTGCATAGC GTCCAGGGAC CCCTACTGCG GATGGATGAA GGAGAGCGGG
    GCATGCATGC AGCTGGTCCT TGGCTCCAAA CTGGCATTTG AACAGGACAT AGAACATGGC
    AATACAGATG GCCTGGGTGA CTGCCAAAAT TCCTTCGTAG CCCTGAATGG ACACTCCAGT
    TCCCTAGTCC CAAGCACCAC CACTTCCGAC TCTCGCGCTC GACAGGGTTA TGATGCTAGG
    GGACGCATGC TGGATTGGAA GGATCCGCTG GGTTCATCTG AAAATACAGA TCCATATGTG
    GCAGTTTCAT CCCATAATCA TCAAGACAAG AAGGGAGTGA TTCGTGAGAG TTACCTGAAG
    GGTCATGATC AGCTGGTGCC GGTCACCCTG TTGGCCATTG CTGTTATTCT TGCTTTTGTG
    ATGGGGGCCA TCTTTTCGGG AATCATAGTG TACTGCATCT GTGACCATCG CCGCAGAGAT
    GTGGCTGTTT CTCAGAGGAA GGAGAAGGAG CTGACCCACT CCCGCCGTGG CTCCATGAGC
    AGCGTTACCA AGCTCAGTGG CCTCTTTGGG GACGCTCAGT CCAAGGAGCC AAAGCCTGAA
    GCTATCCTCA CGCCGCTGAT GCACAATGGC AAACTGGCTA CCCCAGGCAG CACTGCCAAG
    ATGCTAATCA AAGCTGACCA GCACCATCTG GACCTGGCCG CTCTGCCAAC CCCTGAGTCC
    ACCCCAACCC TGCAACAGAA GAGAAAGCCC AGCCGTGGCA GCAGAGAATG GGAGAGAAAC
    CAGAATCTCA TCAATGCCTG CACAAAAGAT ATCCCCCCGA TGGCCTCGCC CGTTATCCCG
    ACTGACCTGC CACTCAGGGC TTCTCCTAGC CACATTCCCA GTGTTGTGGT CCTGCCCATA
    GCCCAGCAAG GCTACCAGCA TGAGTACGTT GACCAGCCAA AAATGAGCGA TGGGGCTCAG
    ATGTCTGTGG AGGACCAGAA CGCCACCCTT GAGTACAAAA CTATCAAGGA CCATCTCAGT
    AGCAAAAGCC CCAGCCACGG GGTTAACCTG GTGGAAAATC TCGACAGCAT GCCACCAAAA
    GTACCCCAGC GGGAAGCTTC CCTCGGGCCC CCGAGCGCCT CGCTGTCGCA GAGCGGCCTG
    AGCAAGCGGC TGGAGATGCA CTCTTCTGCT TACAACGTGG ACTACAAGAG AAGCTACCCT
    ACAAACTCAC TCACAAGAAG TCACCAGACG TCGACCCTCA AGAGAAACAA CACCAACTCC
    TCTAACTCGT CCCACCTCTC CCGCAATCAG AGCTTCGGCC GGGGCGACAA CCCGCCACCG
    GCCCCGCAGC GAGTAGACTC AATACAAGTC CACGCTGCTC AAGCACAGGC TGTGACTGTA
    TCTCGGCAGC CAAGTCTCAG CGCCTACAAC TCGCTGACGA GGTCAGGGTT GAAACGCACA
    CCCTCGCTAA AGCCAGACGT ACCTCCCAAA CCTTCCTTTG CGCCGCTCTC CACGTCCATG
    AAGCCCAACG ACGCATGTAC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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