MED1 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED1
Entrez Gene ID	106897351
Full Name	mediator complex subunit 1
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014957940.1	XP_014813426.1	mediator of RNA polymerase II transcription subunit 1 isoform X2
XM_014957939.1	XP_014813425.1	mediator of RNA polymerase II transcription subunit 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following MED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa76716	XM_014957939.1 Latest version!	Calidris pugnax mediator complex subunit 1 (MED1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa76717	XM_014957940.1 Latest version!	Calidris pugnax mediator complex subunit 1 (MED1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED1 ( XM_014957939.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813425.1 Calidris pugnax mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa76716
Clone ID Related Accession (Same CDS sequence)	XM_014957939.1
Accession Version	XM_014957939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTG GGGGGCACCA ACATCTGGTG AGCTGTTTGG AGACTTTGCA GAAGGCATTA 
AAAGTCTCGT CCCTGCCTGC CATGACAGAC CGCTTGGAGT CCATAGCCAG ACAGAATGGC 
CTTGGATCTC ACCTTAGTGC GAATGGCACT GAATGTTACA TCACTTCAGA CATGTTCTAT 
GTGGAAGTCC AGTTAGATCC TACAGGGCTG CTGTGTGATG TCAAGGTGGC TCACCATGGA 
GAGAACCCCG TGAGCTGTCC AGAATTGGTG CAACATCTGA GAGAGAAAAA TTTTGATGAG 
TTTTCTAAGC ATCTAAGGGG GCTTGTGAAC CTGTATAAGT TGCCGGGAGA CAACAAACTT 
AAAACTAAAA TGTACTTAGC TCTGCAGTCC TTGGAGCTGG ATCTCTCAAA AATGGCAGGG 
ATGTATTGGC AGGCCACCAA CGCAAATCCC CTCGACAAGA TTCTTCATGG CAGTGTTGGC 
TATCTCACCC CCAGGAGCGG AGGTCTCCTG ATGAATCTCA AGTATTATGT CTCACCCTAT 
GATTTGTTTG AGGACGGCAC CGGAGCCCCT GTTATTTTGC ATGAGAACAA TGTTCCTCGG 
TCTTTGGGGA TGAACGTGTC GGTAACAGTT GAGGGAACCA TGAGCATGTA CAAACTTCCA 
ATTGCACCAC TGATTATGGG CTCTCACCCA GTCGACAGCA AAGGAACTCC GTCCTTCTCC 
TCCATCACCA GTGCCAACAG CGTGGATTTA CCGGCTTGTT TCTTCCTGAA ATTCCCACGT 
CCCATTCCCG TGTCTCGTGC TTTCATTCAG AAGCTCCAGG GCTGCACAGG TATTCCCTTG 
TTCGACACCC CACCGACGTT TGTGCCCTTG TATGAGTTGA TCACCCAGTT TGAATTATCC 
AAGGAGGCTG ATCCTCTACC CTTAAACCAC AACATGCGCT TCTATGCAGC TCTTCCAGGA 
CAGCAGCACT GTTACTTTCT GAACAAAGAT GCTCCTCTCC CAGATGGAAG AAGCCTTCAA 
GGAACTCTGA TCAGTAAAAT CGCCTTCCAG CACCCGGGGC GGGTTCCTCT CATCCTCAAT 
CTGATCCGAC ATCAGGTGGC CTACAACACC CTGATTGGCA GCTGTGTCAA GAGAACAGTT 
CTGAAGGAAG ATTCTCCTGG GATCCTGCAG TTTGAAGTTT GTCCTCTCTC TGACTCCTGT 
TTTAGTGTGT CCTTCCAGCA TCCCGTGAAT GACTCCCTGG TGTGTGTGGT AATGGATGTG 
CAAGACTCCA CTCACGTGAA CTGTAAGCTC TACAAAGGGC TGTCCGATGC TCTTATCTGC 
ACAGATGATT TCATTGCCAA AGTTGTTCAA AGGTGTATGT CCATCCCCGT CACCATGAGA 
GCAATTCGTA GAAAAGCAGA AACCATTCAA GCCGACACAC CAGCCTTGTC CCTCATTGCA 
GAGACAGTAG AAGACATGGT GAAGAAAAAC CTCCCCCCAG CCAGCAGCCC AGGGTACGGC 
ATGACCACAG GCAGCAACCC AATGAGTGGT ACCACTACCC CAACCAACAC TTTTCCTGGG 
GGGCCCATCA CTACTTTGTT TAACATGAGC ATAAGCATGA AAGAGAGGCA TGACTCGGTG 
GGCCATGGGG AGGACTTCAG CAAAGTATCT CAGAACCCTA TTCTCACTAG TTTGTTGCAG 
ATCACAGGGA ATGTGGGCTC GACCATTGGC TCAAGTCCAA CCCCTCCCCA CCACACCCCA 
CCACCAGTAT CCTCACCAGC AAGCAACACC AAGAACCACC CCATGCTCAT GAACCTTCTT 
AAAGAGAATC CCCCTCAGGA TTTCTCCACT CTGTACGGGA GCAGCCCTTT GGAAAGGCAG 
AACTCTTCCT CGGGCTCCCC TAGAATGGAA ATGGGCCCTG GGGGGAATAA ACAAAAGAAA 
AAAAAATCCC GCATGCCGGC CGACAAACCC AAACATCAGA CTGAGGATGA TTTCCAGAGG 
GAGCTCTTTT CGATGGATGT TGACTCCCAG AATCCCATTT TTGATGTCAA CATGACGGCA 
GACACCCTGG ACACCCCTCA CATTACTCCA GCTCCCAGCC AGTGCAGCAC TCCTCCCACC 
ACCTACCCCC AGCCCATACC TCACTCCCAG CCCAGTATTC AGAGGATGGT TCGACTCTCC 
AGCTCGGACA GCATCGGGGC CGACGTTACC GATATCCTTT CGGATATAGC AGAGGAGGCT 
TCCAAACTCC CCACCACCAA CGAGGACTGT CCACCCATTG GGACTCCGGT GAGAGACTCT 
TCTAGTTCAG GCCATTCCCA AAGTGCCCTC TTCGACCCGG ATGTTTTTCA GACCAACAAC 
AGCGAGAACC CCTACACGGA CCCCGCAGAC CTGATCGCGG ACGCTGCCGT GAGCCCCAAC 
AGTGATTCTT CCAACCATTT TTTTCCTGAC GGAGTAGATT TCAATCCCGA CTTGCTCAAC 
AGTCAGAGTC AGAGTGGCTT TGGGGAGGAG TATTTTGATG AGAGTAGTCA GAGTGGAGAC 
ACTGACGATT TCAAGGGCTA CGCGTCCCAG GCTCTGTCTA CTTTGGGGGT GCAGGTCTTG 
GGGGCTGATG GGGGGGAAAA TAAATTTAAG GGGAGCAATC AGTCCGATAC GGTAGATTTT 
AGTATTATTG CAGCCGCAAG CAAAGCCCTG GGGTCCTCTG ACATCATGGA GCATCACAGT 
GGAGGTCAGA GCCCTTTGCT GAATACAGGG GATTTAGGAA AAGAGAAGTC CCAGAAACGG 
GTGAAGGAAG GGAATGGCTC TGGTGGGAAC ATGGCAGGTC CCGGGATGGA TGGGAAGCCG 
GGGAAGCGCA GCCGGACGCC GTCCAGTGAT GGTAAAAGTA AAGAGAAACT TCCAAAGAGG 
AAAAAGCAGG AGACAGATGG GAAATCACCA TCTCACAGTT CATCAAACAG GCCTTTCACG 
CCACCAGCCA GCACAGGTGG GTCCAAATCT CCAGGGAGTT CGGGCAGATC TCAGACTCCT 
CCCGGTGTAG CTACTCCTCC TATTCCAAAA ATAACCATCC AGATCCCCAA AGGAACAGTG 
ACTGTTGGCA AACCGTCTTC GCACGGCCAG TATACCAGTA GTGGCTCCGT CACCTCCTCC 
AGCAGCAAAA GCCATCACAG CCATTCTTCT TCCTCCTCCT CTTCTTCCTC CTCTTCGACC 
TCAGGCAAAA TGAAAAGCAG CAAATCGGAA GGGTCTTCTG GCTCAAAGAT GAGCAGCAGC 
CTCTACTCAA GCCAAGGGGG CTCGGGTTCG GGTCAGTCCA AAAGCTCGGC GCAGTCGGTG 
GGAAAGCCTG GATCCTCCCC CATCACCAAA CACGGCCTCA GCAGCGGCTC CGGAGGTACC 
AAGATGAAAC CTCAAGGAAA ACCATCGTCG CTTATGAACC CTTCCATGAG TAAACCAAAC 
ATCTCTCCAT CTCATTCCAG ACCTTCAGGG GGTTCTGACA AGCTTGCTTC TCCCATGAAA 
CCTGTCCCAG GTACTCCCCC ATCATCTAAA GCCAAGTCAC CCATCAGTTC AGGTTCTGGA 
GGCTCCCATA TGTCTGGGAC CGGCTCAAGC TCAAGTATGA AATCGTCTTC AGGAATGGGA 
TCCTCTGGGT CTATGTCACA GAAACCGCCT CCTTCATCCA ATTCGTCCAC GGCGTCTTCA 
TCTTCCTTTT CATCTAGTGG GTCTTCCATG TCTTCATCCC AAAACCAGCA TGGAAGTTCC 
AAAGGCAAGT CTCCGAGCAG AAACAAGAAG CCATCTCTGA CTGCGGTCAT CGACAAACTG 
AAACACGGGG TTGTCACTAG CGGGCCTGGT GGAGATGACC CCATGGATGG ACAGATGGGG 
CCAAGTTCCA ATTCCTCAAG CCATACGATG TCCTCCAAAC ACAACATGTC TGGAGGGGAG 
TTCCAGGGCA AACGGGAGAA GAGCGACAAA GAGAAATCTA AAGTCTCTGT TTCTGGAGGA 
TCTGTTGACT CTTCCAAGAA GACTTCAGAT TCCAAAAACG TTGGAAGCAC TGGAGTGGCC 
AAAATCATCA TCAGCAAACA CGATGGTGGT TCCCCCAGCA TCAAAGCCAA AGTAACCTTG 
CAGAAACCCG GGGAAGGAGG TGGGGACAGC CTGCGGCCTC AGATGGCTTC TTCCAAAAGC 
TACGGGTCCC CCCTCATCAG CGGATCCACC CCGAAACACG AGCGCTGCTC TCCCAGCCAC 
AGCAAATCCC CGGCCTACAC TCCCCAAAAC ATAGACAGCG AGAGCGAGTC GGGCTCTTCC 
ATAGCAGAGA AATCCTACCA GAACAGCCCC AGCTCTGACG ACGGCATCAG GCCTCTGCCG 
GAATATAGCT CAGAAAAACA CAAAAAGCAC AAAAAGGAGA AGAAAAAAGT AAAAGACAAA 
GACCGGGATA GAGACAGGGA GCGAGATAAA GACAGAGACA AGAAGAAATC TCACGGCATG 
AAGCCGGAGA GCTGGTCCAA GTCCCCCATT TCTGCTGAGC AGTCTCTCTC CATGGCCAGC 
AGCACCATCC TCTCGGCCGA GCGACCGGCC CGGGCCAGCC CTGAGTTTCT CATCGGGGAA 
GAAGATGACG ATCTCATGGA TGTTGCTCTA ATTGGCAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014813425.1
CDS	11..4612
Translation	MNSGGHQHLVSCLETLQKALKVSSLPAMTDRLESIARQNGLGSHLSANGTECYITSDMFYVEVQLDPTGLLCDVKVAHHGENPVSCPELVQHLREKNFDEFSKHLRGLVNLYKLPGDNKLKTKMYLALQSLELDLSKMAGMYWQATNANPLDKILHGSVGYLTPRSGGLLMNLKYYVSPYDLFEDGTGAPVILHENNVPRSLGMNVSVTVEGTMSMYKLPIAPLIMGSHPVDSKGTPSFSSITSANSVDLPACFFLKFPRPIPVSRAFIQKLQGCTGIPLFDTPPTFVPLYELITQFELSKEADPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLISKIAFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTVLKEDSPGILQFEVCPLSDSCFSVSFQHPVNDSLVCVVMDVQDSTHVNCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGPITTLFNMSISMKERHDSVGHGEDFSKVSQNPILTSLLQITGNVGSTIGSSPTPPHHTPPPVSSPASNTKNHPMLMNLLKENPPQDFSTLYGSSPLERQNSSSGSPRMEMGPGGNKQKKKKSRMPADKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPIPHSQPSIQRMVRLSSSDSIGADVTDILSDIAEEASKLPTTNEDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNSENPYTDPADLIADAAVSPNSDSSNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDTDDFKGYASQALSTLGVQVLGADGGENKFKGSNQSDTVDFSIIAAASKALGSSDIMEHHSGGQSPLLNTGDLGKEKSQKRVKEGNGSGGNMAGPGMDGKPGKRSRTPSSDGKSKEKLPKRKKQETDGKSPSHSSSNRPFTPPASTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVTVGKPSSHGQYTSSGSVTSSSSKSHHSHSSSSSSSSSSSTSGKMKSSKSEGSSGSKMSSSLYSSQGGSGSGQSKSSAQSVGKPGSSPITKHGLSSGSGGTKMKPQGKPSSLMNPSMSKPNISPSHSRPSGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTGSSSSMKSSSGMGSSGSMSQKPPPSSNSSTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGDDPMDGQMGPSSNSSSHTMSSKHNMSGGEFQGKREKSDKEKSKVSVSGGSVDSSKKTSDSKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGEGGGDSLRPQMASSKSYGSPLISGSTPKHERCSPSHSKSPAYTPQNIDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSSEKHKKHKKEKKKVKDKDRDRDRERDKDRDKKKSHGMKPESWSKSPISAEQSLSMASSTILSAERPARASPEFLIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_014957939.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mediator complex subunit 1 (MED1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014957939.1

ORF Insert Sequence:

ATGAACTCTG GGGGGCACCA ACATCTGGTG AGCTGTTTGG AGACTTTGCA GAAGGCATTA 
AAAGTCTCGT CCCTGCCTGC CATGACAGAC CGCTTGGAGT CCATAGCCAG ACAGAATGGC 
CTTGGATCTC ACCTTAGTGC GAATGGCACT GAATGTTACA TCACTTCAGA CATGTTCTAT 
GTGGAAGTCC AGTTAGATCC TACAGGGCTG CTGTGTGATG TCAAGGTGGC TCACCATGGA 
GAGAACCCCG TGAGCTGTCC AGAATTGGTG CAACATCTGA GAGAGAAAAA TTTTGATGAG 
TTTTCTAAGC ATCTAAGGGG GCTTGTGAAC CTGTATAAGT TGCCGGGAGA CAACAAACTT 
AAAACTAAAA TGTACTTAGC TCTGCAGTCC TTGGAGCTGG ATCTCTCAAA AATGGCAGGG 
ATGTATTGGC AGGCCACCAA CGCAAATCCC CTCGACAAGA TTCTTCATGG CAGTGTTGGC 
TATCTCACCC CCAGGAGCGG AGGTCTCCTG ATGAATCTCA AGTATTATGT CTCACCCTAT 
GATTTGTTTG AGGACGGCAC CGGAGCCCCT GTTATTTTGC ATGAGAACAA TGTTCCTCGG 
TCTTTGGGGA TGAACGTGTC GGTAACAGTT GAGGGAACCA TGAGCATGTA CAAACTTCCA 
ATTGCACCAC TGATTATGGG CTCTCACCCA GTCGACAGCA AAGGAACTCC GTCCTTCTCC 
TCCATCACCA GTGCCAACAG CGTGGATTTA CCGGCTTGTT TCTTCCTGAA ATTCCCACGT 
CCCATTCCCG TGTCTCGTGC TTTCATTCAG AAGCTCCAGG GCTGCACAGG TATTCCCTTG 
TTCGACACCC CACCGACGTT TGTGCCCTTG TATGAGTTGA TCACCCAGTT TGAATTATCC 
AAGGAGGCTG ATCCTCTACC CTTAAACCAC AACATGCGCT TCTATGCAGC TCTTCCAGGA 
CAGCAGCACT GTTACTTTCT GAACAAAGAT GCTCCTCTCC CAGATGGAAG AAGCCTTCAA 
GGAACTCTGA TCAGTAAAAT CGCCTTCCAG CACCCGGGGC GGGTTCCTCT CATCCTCAAT 
CTGATCCGAC ATCAGGTGGC CTACAACACC CTGATTGGCA GCTGTGTCAA GAGAACAGTT 
CTGAAGGAAG ATTCTCCTGG GATCCTGCAG TTTGAAGTTT GTCCTCTCTC TGACTCCTGT 
TTTAGTGTGT CCTTCCAGCA TCCCGTGAAT GACTCCCTGG TGTGTGTGGT AATGGATGTG 
CAAGACTCCA CTCACGTGAA CTGTAAGCTC TACAAAGGGC TGTCCGATGC TCTTATCTGC 
ACAGATGATT TCATTGCCAA AGTTGTTCAA AGGTGTATGT CCATCCCCGT CACCATGAGA 
GCAATTCGTA GAAAAGCAGA AACCATTCAA GCCGACACAC CAGCCTTGTC CCTCATTGCA 
GAGACAGTAG AAGACATGGT GAAGAAAAAC CTCCCCCCAG CCAGCAGCCC AGGGTACGGC 
ATGACCACAG GCAGCAACCC AATGAGTGGT ACCACTACCC CAACCAACAC TTTTCCTGGG 
GGGCCCATCA CTACTTTGTT TAACATGAGC ATAAGCATGA AAGAGAGGCA TGACTCGGTG 
GGCCATGGGG AGGACTTCAG CAAAGTATCT CAGAACCCTA TTCTCACTAG TTTGTTGCAG 
ATCACAGGGA ATGTGGGCTC GACCATTGGC TCAAGTCCAA CCCCTCCCCA CCACACCCCA 
CCACCAGTAT CCTCACCAGC AAGCAACACC AAGAACCACC CCATGCTCAT GAACCTTCTT 
AAAGAGAATC CCCCTCAGGA TTTCTCCACT CTGTACGGGA GCAGCCCTTT GGAAAGGCAG 
AACTCTTCCT CGGGCTCCCC TAGAATGGAA ATGGGCCCTG GGGGGAATAA ACAAAAGAAA 
AAAAAATCCC GCATGCCGGC CGACAAACCC AAACATCAGA CTGAGGATGA TTTCCAGAGG 
GAGCTCTTTT CGATGGATGT TGACTCCCAG AATCCCATTT TTGATGTCAA CATGACGGCA 
GACACCCTGG ACACCCCTCA CATTACTCCA GCTCCCAGCC AGTGCAGCAC TCCTCCCACC 
ACCTACCCCC AGCCCATACC TCACTCCCAG CCCAGTATTC AGAGGATGGT TCGACTCTCC 
AGCTCGGACA GCATCGGGGC CGACGTTACC GATATCCTTT CGGATATAGC AGAGGAGGCT 
TCCAAACTCC CCACCACCAA CGAGGACTGT CCACCCATTG GGACTCCGGT GAGAGACTCT 
TCTAGTTCAG GCCATTCCCA AAGTGCCCTC TTCGACCCGG ATGTTTTTCA GACCAACAAC 
AGCGAGAACC CCTACACGGA CCCCGCAGAC CTGATCGCGG ACGCTGCCGT GAGCCCCAAC 
AGTGATTCTT CCAACCATTT TTTTCCTGAC GGAGTAGATT TCAATCCCGA CTTGCTCAAC 
AGTCAGAGTC AGAGTGGCTT TGGGGAGGAG TATTTTGATG AGAGTAGTCA GAGTGGAGAC 
ACTGACGATT TCAAGGGCTA CGCGTCCCAG GCTCTGTCTA CTTTGGGGGT GCAGGTCTTG 
GGGGCTGATG GGGGGGAAAA TAAATTTAAG GGGAGCAATC AGTCCGATAC GGTAGATTTT 
AGTATTATTG CAGCCGCAAG CAAAGCCCTG GGGTCCTCTG ACATCATGGA GCATCACAGT 
GGAGGTCAGA GCCCTTTGCT GAATACAGGG GATTTAGGAA AAGAGAAGTC CCAGAAACGG 
GTGAAGGAAG GGAATGGCTC TGGTGGGAAC ATGGCAGGTC CCGGGATGGA TGGGAAGCCG 
GGGAAGCGCA GCCGGACGCC GTCCAGTGAT GGTAAAAGTA AAGAGAAACT TCCAAAGAGG 
AAAAAGCAGG AGACAGATGG GAAATCACCA TCTCACAGTT CATCAAACAG GCCTTTCACG 
CCACCAGCCA GCACAGGTGG GTCCAAATCT CCAGGGAGTT CGGGCAGATC TCAGACTCCT 
CCCGGTGTAG CTACTCCTCC TATTCCAAAA ATAACCATCC AGATCCCCAA AGGAACAGTG 
ACTGTTGGCA AACCGTCTTC GCACGGCCAG TATACCAGTA GTGGCTCCGT CACCTCCTCC 
AGCAGCAAAA GCCATCACAG CCATTCTTCT TCCTCCTCCT CTTCTTCCTC CTCTTCGACC 
TCAGGCAAAA TGAAAAGCAG CAAATCGGAA GGGTCTTCTG GCTCAAAGAT GAGCAGCAGC 
CTCTACTCAA GCCAAGGGGG CTCGGGTTCG GGTCAGTCCA AAAGCTCGGC GCAGTCGGTG 
GGAAAGCCTG GATCCTCCCC CATCACCAAA CACGGCCTCA GCAGCGGCTC CGGAGGTACC 
AAGATGAAAC CTCAAGGAAA ACCATCGTCG CTTATGAACC CTTCCATGAG TAAACCAAAC 
ATCTCTCCAT CTCATTCCAG ACCTTCAGGG GGTTCTGACA AGCTTGCTTC TCCCATGAAA 
CCTGTCCCAG GTACTCCCCC ATCATCTAAA GCCAAGTCAC CCATCAGTTC AGGTTCTGGA 
GGCTCCCATA TGTCTGGGAC CGGCTCAAGC TCAAGTATGA AATCGTCTTC AGGAATGGGA 
TCCTCTGGGT CTATGTCACA GAAACCGCCT CCTTCATCCA ATTCGTCCAC GGCGTCTTCA 
TCTTCCTTTT CATCTAGTGG GTCTTCCATG TCTTCATCCC AAAACCAGCA TGGAAGTTCC 
AAAGGCAAGT CTCCGAGCAG AAACAAGAAG CCATCTCTGA CTGCGGTCAT CGACAAACTG 
AAACACGGGG TTGTCACTAG CGGGCCTGGT GGAGATGACC CCATGGATGG ACAGATGGGG 
CCAAGTTCCA ATTCCTCAAG CCATACGATG TCCTCCAAAC ACAACATGTC TGGAGGGGAG 
TTCCAGGGCA AACGGGAGAA GAGCGACAAA GAGAAATCTA AAGTCTCTGT TTCTGGAGGA 
TCTGTTGACT CTTCCAAGAA GACTTCAGAT TCCAAAAACG TTGGAAGCAC TGGAGTGGCC 
AAAATCATCA TCAGCAAACA CGATGGTGGT TCCCCCAGCA TCAAAGCCAA AGTAACCTTG 
CAGAAACCCG GGGAAGGAGG TGGGGACAGC CTGCGGCCTC AGATGGCTTC TTCCAAAAGC 
TACGGGTCCC CCCTCATCAG CGGATCCACC CCGAAACACG AGCGCTGCTC TCCCAGCCAC 
AGCAAATCCC CGGCCTACAC TCCCCAAAAC ATAGACAGCG AGAGCGAGTC GGGCTCTTCC 
ATAGCAGAGA AATCCTACCA GAACAGCCCC AGCTCTGACG ACGGCATCAG GCCTCTGCCG 
GAATATAGCT CAGAAAAACA CAAAAAGCAC AAAAAGGAGA AGAAAAAAGT AAAAGACAAA 
GACCGGGATA GAGACAGGGA GCGAGATAAA GACAGAGACA AGAAGAAATC TCACGGCATG 
AAGCCGGAGA GCTGGTCCAA GTCCCCCATT TCTGCTGAGC AGTCTCTCTC CATGGCCAGC 
AGCACCATCC TCTCGGCCGA GCGACCGGCC CGGGCCAGCC CTGAGTTTCT CATCGGGGAA 
GAAGATGACG ATCTCATGGA TGTTGCTCTA ATTGGCAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( XM_014957940.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014813426.1 Calidris pugnax mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa76717
Clone ID Related Accession (Same CDS sequence)	XM_014957940.1
Accession Version	XM_014957940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mediator of RNA polymerase II transcription subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGG CGCCGGGCGG TGCCGAGGAA ACGGAGAAGC TGAATAAGAT GAGTACCCTC 
TTAGAAAGAC TTCATGCAAA GTACAACCAA AACAGGCCTT GGACAGAAAC CATGAAGCTG 
GTCCGTCAAG TCATGGAAAA GCGAATTGTG ATGAACTCTG GGGGGCACCA ACATCTGGTG 
AGCTGTTTGG AGACTTTGCA GAAGGCATTA AAAGTCTCGT CTCTGCCTGC CATGACAGAC 
CGCTTGGAGT CCATAGCCAG ACAGAATGGC CTTGGATCTC ACCTTAGCGC GAATGGCACT 
GAATGTTACA TCACTTCAGA CATGTTCTAT GTGGAAGTCC AGTTAGATCC TACAGGGCTG 
CTGTGTGATG TCAAGGTGGC TCACCATGGA GAGAACCCCG TGAGCTGTCC AGAATTGGTG 
CAACATCTGA GAGAGAAAAA TTTTGATGAA TTTTCTAAGC ATCTAAGGGG GCTTGTGAAC 
CTGTATAAGT TGCCGGGAGA CAACAAACTT AAAACTAAAA TGTACTTAGC TCTGCAGTCC 
TTGGAGCTGG ATCTCTCAAA AATGGCAGGG ATGTATTGGC AAGCCACCAA CGCAAATCCC 
CTCGACAAGA TTCTTCATGG CAGTGTTGGC TATCTCACCC CCAGGAGCGG AGGTCTCCTG 
ATGAATCTCA AGTATTATGT CTCACCCTAT GATTTGTTTG AGGACGGCAC CGGAGCCCCT 
GTTATTTTGC ATGAGAACAA TGTTCCTCGG TCTTTGGGGA TGAACGTGTC GGTAACAGTT 
GAGGGAACCA TGAGCATGTA CAAACTTCCA ATTGCACCAC TGATTATGGG CTCTCACCCA 
GTTGACAGCA AAGGAACTCC GTCCTTCTCC TCCATCACCA GTGCCAACAG CGTGGATTTA 
CCGGCTTGTT TCTTCCTGAA ATTCCCACGT CCCATTCCCG TGTCTCGTGC TTTCATTCAG 
AAGCTCCAGG GCTGCACAGG TATTCCCTTG TTCGACACCC CACCGACGTT TGTGCCCTTG 
TATGAGTTGA TCACCCAGTT TGAATTATCC AAGGAGGCTG ATCCTCTACC CTTAAACCAC 
AACATGCGCT TCTATGCAGC TCTTCCAGGA CAGCAGCACT GTTACTTTCT GAACAAAGAT 
GCTCCTCTCC CAGATGGAAG AAGCCTTCAA GGAACTCTGA TCAGTAAAAT CGCCTTCCAG 
CACCCGGGGC GGGTTCCTCT CATCCTCAAT CTGATCCGAC ATCAGGTGGC CTACAACACC 
CTGATTGGCA GCTGTGTCAA GAGAACAGTT CTGAAGGAAG ATTCTCCTGG GATCCTGCAG 
TTTGAAGTTT GTCCTCTCTC TGACTCCTGT TTTAGTGTGT CCTTCCAGCA TCCCGTGAAT 
GACTCCCTGG TGTGTGTGGT AATGGATGTG CAAGACTCCA CTCACGTGAA CTGTAAGCTC 
TACAAAGGGC TGTCCGATGC TCTTATCTGC ACAGATGATT TCATTGCCAA AGTTGTTCAA 
AGGTGTATGT CCATCCCCGT CACCATGAGA GCAATTCGTA GAAAAGCAGA AACCATTCAA 
GCCGACACAC CAGCCTTGTC CCTCATTGCA GAGACAGTAG AAGACATGGT GAAGAAAAAC 
CTCCCCCCAG CCAGCAGCCC AGGGTACGGC ATGACCACAG GCAGCAACCC AATGAGTGGT 
ACCACTACCC CAACCAACAC TTTTCCTGGG GGGCCCATCA CTACTTTGTT TAACATGAGC 
ATAAGCATGA AAGAGAGGCA TGACTCGGTG GGCCATGGGG AGGACTTCAG CAAAGTATCT 
CAGAACCCTA TTCTCACTAG TTTGTTGCAG ATCACAGGGA ATGTGGGCTC GACCATTGGC 
TCAAGTCCAA CCCCTCCCCA CCACACCCCA CCACCAGTAT CCTCACCAGC AAGCAACACC 
AAGAACCACC CCATGCTCAT GAACCTTCTT AAAGAGAATC CCCCTCAGGA TTTCTCCACT 
CTGTACGGGA GCAGCCCTTT GGAAAGGCAG AACTCTTCCT CGGGCTCCCC TAGAATGGAA 
ATGGGCCCTG GGGGGAATAA ACAAAAGAAA AAAAAATCCC GCATGCCGGC CGACAAACCC 
AAACATCAGA CTGAGGATGA TTTCCAGAGG GAGCTCTTTT CGATGGATGT TGACTCCCAG 
AATCCCATTT TTGATGTCAA CATGACGGCA GACACCCTGG ACACCCCTCA CATTACTCCA 
GCTCCCAGCC AGTGCAGCAC TCCTCCCACC ACCTACCCCC AGCCCATACC TCACTCCCAG 
CCCAGTATTC AGAGGATGGT TCGACTCTCC AGCTCGGACA GCATCGGGGC CGACGTTACC 
GATATCCTTT CGGATATAGC AGAGGAGGCT TCCAAACTCC CCACCACCAA CGAGGACTGT 
CCACCCATTG GGACTCCGGT GAGAGACTCT TCTAGTTCAG GCCATTCCCA AAGTGCCCTC 
TTCGACCCGG ATGTTTTTCA GACCAACAAC AGCGAGAACC CCTACACGGA CCCCGCAGAC 
CTGATCGCGG ACGCTGCCGT GAGCCCCAAC AGTGATTCTT CCAACCATTT TTTTCCTGAC 
GGAGTAGATT TCAATCCCGA CTTGCTCAAC AGTCAGAGTC AGAGTGGCTT TGGGGAGGAG 
TATTTTGATG AGAGTAGTCA GAGTGGAGAC ACTGACGATT TCAAGGGCTA CGCGTCCCAG 
GCTCTGTCTA CTTTGGGGGT GCAGGTCTTG GGGGCTGATG GGGGGGAAAA TAAATTTAAG 
GGGAGCAATC AGTCCGATAC GGTAGATTTT AGTATTATTG CAGCCGCAAG CAAAGCCCTG 
GGGTCCTCTG ACATCATGGA GCATCACAGT GGAGGTCAGA GCCCTTTGCT GAATACAGGG 
GATTTAGGAA AAGAGAAGTC CCAGAAACGG GTGAAGGAAG GGAATGGCTC TGGTGGGAAC 
ATGGCAGGTC CCGGGATGGA TGGGAAGCCG GGGAAGCGCA GCCGGACGCC GTCCAGTGAT 
GGTAAAAGTA AAGAGAAACT TCCAAAGAGG AAAAAGCAGG AGACAGATGG GAAATCACCA 
TCTCACAGTT CATCAAACAG GCCTTTCACG CCACCAGCCA GCACAGGTGG GTCCAAATCT 
CCAGGGAGTT CGGGCAGATC TCAGACTCCT CCCGGTGTAG CTACTCCTCC TATTCCAAAA 
ATAACCATCC AGATCCCCAA AGGAACAGTG ACTGTTGGCA AACCGTCTTC GCACGGCCAG 
TATACCAGTA GTGGCTCCGT CACCTCCTCC AGCAGCAAAA GCCATCACAG CCATTCTTCT 
TCCTCCTCCT CTTCTTCCTC CTCTTCGACC TCAGGCAAAA TGAAAAGCAG CAAATCGGAA 
GGGTCTTCTG GCTCAAAGAT GAGCAGCAGC CTCTACTCAA GCCAAGGGGG CTCGGGTTCG 
GGTCAGTCCA AAAGCTCGGC GCAGTCGGTG GGAAAGCCTG GATCCTCCCC CATCACCAAA 
CACGGCCTCA GCAGCGGCTC CGGAGGTACC AAGATGAAAC CTCAAGGAAA ACCATCGTCG 
CTTATGAACC CTTCCATGAG TAAACCAAAC ATCTCTCCAT CTCATTCCAG ACCTTCAGGG 
GGTTCTGACA AGCTTGCTTC TCCCATGAAA CCTGTCCCAG GTACTCCCCC ATCATCTAAA 
GCCAAGTCAC CCATCAGTTC AGGTTCTGGA GGCTCCCATA TGTCTGGGAC CGGCTCAAGC 
TCAAGTATGA AATCGTCTTC AGGAATGGGA TCCTCTGGGT CTATGTCACA GAAACCGCCT 
CCTTCATCCA ATTCGTCCAC GGCGTCTTCA TCTTCCTTTT CATCTAGTGG GTCTTCCATG 
TCTTCATCCC AAAACCAGCA TGGAAGTTCC AAAGGCAAGT CTCCGAGCAG AAACAAGAAG 
CCATCTCTGA CTGCGGTCAT CGACAAACTG AAACACGGGG TTGTCACTAG CGGGCCTGGT 
GGAGATGACC CCATGGATGG ACAGATGGGG CCAAGTTCCA ATTCCTCAAG CCATACGATG 
TCCTCCAAAC ACAACATGTC TGGAGGGGAG TTCCAGGGCA AACGGGAGAA GAGCGACAAA 
GAGAAATCTA AAGTCTCTGT TTCTGGAGGA TCTGTTGACT CTTCCAAGAA GACTTCAGAT 
TCCAAAAACG TTGGAAGCAC TGGAGTGGCC AAAATCATCA TCAGCAAACA CGATGGTGGT 
TCCCCCAGCA TCAAAGCCAA AGTAACCTTG CAGAAACCCG GGGAAGGAGG TGGGGACAGC 
CTGCGGCCTC AGATGGCTTC TTCCAAAAGC TACGGGTCCC CCCTCATCAG CGGATCCACC 
CCGAAACACG AGCGCTGCTC TCCCAGCCAC AGCAAATCCC CGGCCTACAC TCCCCAAAAC 
ATAGACAGCG AGAGCGAGTC GGGCTCTTCC ATAGCAGAGA AATCCTACCA GAACAGCCCC 
AGCTCTGACG ACGGCATCAG GCCTCTGCCG GAATATAGCT CAGAAAAACA CAAAAAGCAC 
AAAAAGGAGA AGAAAAAAGT AAAAGACAAA GACCGGGATA GAGACAGGGA GCGAGATAAA 
GACAGAGACA AGAAGAAATC TCACGGCATG AAGCCGGAGA GCTGGTCCAA GTCCCCCATT 
TCTGCTGAGC AGTCTCTCTC CATGGCCAGC AGCACCATCC TCTCGGCCGA GCGACCGGCC 
CGGGCCAGCC CTGAGTTTCT CATCGGGGAA GAAGATGACG ATCTCATGGA TGTTGCTCTA 
ATTGGCAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014813426.1
CDS	1..4752
Translation	MKAAPGGAEETEKLNKMSTLLERLHAKYNQNRPWTETMKLVRQVMEKRIVMNSGGHQHLVSCLETLQKALKVSSLPAMTDRLESIARQNGLGSHLSANGTECYITSDMFYVEVQLDPTGLLCDVKVAHHGENPVSCPELVQHLREKNFDEFSKHLRGLVNLYKLPGDNKLKTKMYLALQSLELDLSKMAGMYWQATNANPLDKILHGSVGYLTPRSGGLLMNLKYYVSPYDLFEDGTGAPVILHENNVPRSLGMNVSVTVEGTMSMYKLPIAPLIMGSHPVDSKGTPSFSSITSANSVDLPACFFLKFPRPIPVSRAFIQKLQGCTGIPLFDTPPTFVPLYELITQFELSKEADPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLISKIAFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTVLKEDSPGILQFEVCPLSDSCFSVSFQHPVNDSLVCVVMDVQDSTHVNCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGPITTLFNMSISMKERHDSVGHGEDFSKVSQNPILTSLLQITGNVGSTIGSSPTPPHHTPPPVSSPASNTKNHPMLMNLLKENPPQDFSTLYGSSPLERQNSSSGSPRMEMGPGGNKQKKKKSRMPADKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPIPHSQPSIQRMVRLSSSDSIGADVTDILSDIAEEASKLPTTNEDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNSENPYTDPADLIADAAVSPNSDSSNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDTDDFKGYASQALSTLGVQVLGADGGENKFKGSNQSDTVDFSIIAAASKALGSSDIMEHHSGGQSPLLNTGDLGKEKSQKRVKEGNGSGGNMAGPGMDGKPGKRSRTPSSDGKSKEKLPKRKKQETDGKSPSHSSSNRPFTPPASTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVTVGKPSSHGQYTSSGSVTSSSSKSHHSHSSSSSSSSSSSTSGKMKSSKSEGSSGSKMSSSLYSSQGGSGSGQSKSSAQSVGKPGSSPITKHGLSSGSGGTKMKPQGKPSSLMNPSMSKPNISPSHSRPSGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTGSSSSMKSSSGMGSSGSMSQKPPPSSNSSTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGDDPMDGQMGPSSNSSSHTMSSKHNMSGGEFQGKREKSDKEKSKVSVSGGSVDSSKKTSDSKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGEGGGDSLRPQMASSKSYGSPLISGSTPKHERCSPSHSKSPAYTPQNIDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSSEKHKKHKKEKKKVKDKDRDRDRERDKDRDKKKSHGMKPESWSKSPISAEQSLSMASSTILSAERPARASPEFLIGEEDDDLMDVALIGN

Target ORF information:

RefSeq Version	XM_014957940.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mediator complex subunit 1 (MED1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014957940.1

ORF Insert Sequence:

ATGAAGGCGG CGCCGGGCGG TGCCGAGGAA ACGGAGAAGC TGAATAAGAT GAGTACCCTC 
TTAGAAAGAC TTCATGCAAA GTACAACCAA AACAGGCCTT GGACAGAAAC CATGAAGCTG 
GTCCGTCAAG TCATGGAAAA GCGAATTGTG ATGAACTCTG GGGGGCACCA ACATCTGGTG 
AGCTGTTTGG AGACTTTGCA GAAGGCATTA AAAGTCTCGT CTCTGCCTGC CATGACAGAC 
CGCTTGGAGT CCATAGCCAG ACAGAATGGC CTTGGATCTC ACCTTAGCGC GAATGGCACT 
GAATGTTACA TCACTTCAGA CATGTTCTAT GTGGAAGTCC AGTTAGATCC TACAGGGCTG 
CTGTGTGATG TCAAGGTGGC TCACCATGGA GAGAACCCCG TGAGCTGTCC AGAATTGGTG 
CAACATCTGA GAGAGAAAAA TTTTGATGAA TTTTCTAAGC ATCTAAGGGG GCTTGTGAAC 
CTGTATAAGT TGCCGGGAGA CAACAAACTT AAAACTAAAA TGTACTTAGC TCTGCAGTCC 
TTGGAGCTGG ATCTCTCAAA AATGGCAGGG ATGTATTGGC AAGCCACCAA CGCAAATCCC 
CTCGACAAGA TTCTTCATGG CAGTGTTGGC TATCTCACCC CCAGGAGCGG AGGTCTCCTG 
ATGAATCTCA AGTATTATGT CTCACCCTAT GATTTGTTTG AGGACGGCAC CGGAGCCCCT 
GTTATTTTGC ATGAGAACAA TGTTCCTCGG TCTTTGGGGA TGAACGTGTC GGTAACAGTT 
GAGGGAACCA TGAGCATGTA CAAACTTCCA ATTGCACCAC TGATTATGGG CTCTCACCCA 
GTTGACAGCA AAGGAACTCC GTCCTTCTCC TCCATCACCA GTGCCAACAG CGTGGATTTA 
CCGGCTTGTT TCTTCCTGAA ATTCCCACGT CCCATTCCCG TGTCTCGTGC TTTCATTCAG 
AAGCTCCAGG GCTGCACAGG TATTCCCTTG TTCGACACCC CACCGACGTT TGTGCCCTTG 
TATGAGTTGA TCACCCAGTT TGAATTATCC AAGGAGGCTG ATCCTCTACC CTTAAACCAC 
AACATGCGCT TCTATGCAGC TCTTCCAGGA CAGCAGCACT GTTACTTTCT GAACAAAGAT 
GCTCCTCTCC CAGATGGAAG AAGCCTTCAA GGAACTCTGA TCAGTAAAAT CGCCTTCCAG 
CACCCGGGGC GGGTTCCTCT CATCCTCAAT CTGATCCGAC ATCAGGTGGC CTACAACACC 
CTGATTGGCA GCTGTGTCAA GAGAACAGTT CTGAAGGAAG ATTCTCCTGG GATCCTGCAG 
TTTGAAGTTT GTCCTCTCTC TGACTCCTGT TTTAGTGTGT CCTTCCAGCA TCCCGTGAAT 
GACTCCCTGG TGTGTGTGGT AATGGATGTG CAAGACTCCA CTCACGTGAA CTGTAAGCTC 
TACAAAGGGC TGTCCGATGC TCTTATCTGC ACAGATGATT TCATTGCCAA AGTTGTTCAA 
AGGTGTATGT CCATCCCCGT CACCATGAGA GCAATTCGTA GAAAAGCAGA AACCATTCAA 
GCCGACACAC CAGCCTTGTC CCTCATTGCA GAGACAGTAG AAGACATGGT GAAGAAAAAC 
CTCCCCCCAG CCAGCAGCCC AGGGTACGGC ATGACCACAG GCAGCAACCC AATGAGTGGT 
ACCACTACCC CAACCAACAC TTTTCCTGGG GGGCCCATCA CTACTTTGTT TAACATGAGC 
ATAAGCATGA AAGAGAGGCA TGACTCGGTG GGCCATGGGG AGGACTTCAG CAAAGTATCT 
CAGAACCCTA TTCTCACTAG TTTGTTGCAG ATCACAGGGA ATGTGGGCTC GACCATTGGC 
TCAAGTCCAA CCCCTCCCCA CCACACCCCA CCACCAGTAT CCTCACCAGC AAGCAACACC 
AAGAACCACC CCATGCTCAT GAACCTTCTT AAAGAGAATC CCCCTCAGGA TTTCTCCACT 
CTGTACGGGA GCAGCCCTTT GGAAAGGCAG AACTCTTCCT CGGGCTCCCC TAGAATGGAA 
ATGGGCCCTG GGGGGAATAA ACAAAAGAAA AAAAAATCCC GCATGCCGGC CGACAAACCC 
AAACATCAGA CTGAGGATGA TTTCCAGAGG GAGCTCTTTT CGATGGATGT TGACTCCCAG 
AATCCCATTT TTGATGTCAA CATGACGGCA GACACCCTGG ACACCCCTCA CATTACTCCA 
GCTCCCAGCC AGTGCAGCAC TCCTCCCACC ACCTACCCCC AGCCCATACC TCACTCCCAG 
CCCAGTATTC AGAGGATGGT TCGACTCTCC AGCTCGGACA GCATCGGGGC CGACGTTACC 
GATATCCTTT CGGATATAGC AGAGGAGGCT TCCAAACTCC CCACCACCAA CGAGGACTGT 
CCACCCATTG GGACTCCGGT GAGAGACTCT TCTAGTTCAG GCCATTCCCA AAGTGCCCTC 
TTCGACCCGG ATGTTTTTCA GACCAACAAC AGCGAGAACC CCTACACGGA CCCCGCAGAC 
CTGATCGCGG ACGCTGCCGT GAGCCCCAAC AGTGATTCTT CCAACCATTT TTTTCCTGAC 
GGAGTAGATT TCAATCCCGA CTTGCTCAAC AGTCAGAGTC AGAGTGGCTT TGGGGAGGAG 
TATTTTGATG AGAGTAGTCA GAGTGGAGAC ACTGACGATT TCAAGGGCTA CGCGTCCCAG 
GCTCTGTCTA CTTTGGGGGT GCAGGTCTTG GGGGCTGATG GGGGGGAAAA TAAATTTAAG 
GGGAGCAATC AGTCCGATAC GGTAGATTTT AGTATTATTG CAGCCGCAAG CAAAGCCCTG 
GGGTCCTCTG ACATCATGGA GCATCACAGT GGAGGTCAGA GCCCTTTGCT GAATACAGGG 
GATTTAGGAA AAGAGAAGTC CCAGAAACGG GTGAAGGAAG GGAATGGCTC TGGTGGGAAC 
ATGGCAGGTC CCGGGATGGA TGGGAAGCCG GGGAAGCGCA GCCGGACGCC GTCCAGTGAT 
GGTAAAAGTA AAGAGAAACT TCCAAAGAGG AAAAAGCAGG AGACAGATGG GAAATCACCA 
TCTCACAGTT CATCAAACAG GCCTTTCACG CCACCAGCCA GCACAGGTGG GTCCAAATCT 
CCAGGGAGTT CGGGCAGATC TCAGACTCCT CCCGGTGTAG CTACTCCTCC TATTCCAAAA 
ATAACCATCC AGATCCCCAA AGGAACAGTG ACTGTTGGCA AACCGTCTTC GCACGGCCAG 
TATACCAGTA GTGGCTCCGT CACCTCCTCC AGCAGCAAAA GCCATCACAG CCATTCTTCT 
TCCTCCTCCT CTTCTTCCTC CTCTTCGACC TCAGGCAAAA TGAAAAGCAG CAAATCGGAA 
GGGTCTTCTG GCTCAAAGAT GAGCAGCAGC CTCTACTCAA GCCAAGGGGG CTCGGGTTCG 
GGTCAGTCCA AAAGCTCGGC GCAGTCGGTG GGAAAGCCTG GATCCTCCCC CATCACCAAA 
CACGGCCTCA GCAGCGGCTC CGGAGGTACC AAGATGAAAC CTCAAGGAAA ACCATCGTCG 
CTTATGAACC CTTCCATGAG TAAACCAAAC ATCTCTCCAT CTCATTCCAG ACCTTCAGGG 
GGTTCTGACA AGCTTGCTTC TCCCATGAAA CCTGTCCCAG GTACTCCCCC ATCATCTAAA 
GCCAAGTCAC CCATCAGTTC AGGTTCTGGA GGCTCCCATA TGTCTGGGAC CGGCTCAAGC 
TCAAGTATGA AATCGTCTTC AGGAATGGGA TCCTCTGGGT CTATGTCACA GAAACCGCCT 
CCTTCATCCA ATTCGTCCAC GGCGTCTTCA TCTTCCTTTT CATCTAGTGG GTCTTCCATG 
TCTTCATCCC AAAACCAGCA TGGAAGTTCC AAAGGCAAGT CTCCGAGCAG AAACAAGAAG 
CCATCTCTGA CTGCGGTCAT CGACAAACTG AAACACGGGG TTGTCACTAG CGGGCCTGGT 
GGAGATGACC CCATGGATGG ACAGATGGGG CCAAGTTCCA ATTCCTCAAG CCATACGATG 
TCCTCCAAAC ACAACATGTC TGGAGGGGAG TTCCAGGGCA AACGGGAGAA GAGCGACAAA 
GAGAAATCTA AAGTCTCTGT TTCTGGAGGA TCTGTTGACT CTTCCAAGAA GACTTCAGAT 
TCCAAAAACG TTGGAAGCAC TGGAGTGGCC AAAATCATCA TCAGCAAACA CGATGGTGGT 
TCCCCCAGCA TCAAAGCCAA AGTAACCTTG CAGAAACCCG GGGAAGGAGG TGGGGACAGC 
CTGCGGCCTC AGATGGCTTC TTCCAAAAGC TACGGGTCCC CCCTCATCAG CGGATCCACC 
CCGAAACACG AGCGCTGCTC TCCCAGCCAC AGCAAATCCC CGGCCTACAC TCCCCAAAAC 
ATAGACAGCG AGAGCGAGTC GGGCTCTTCC ATAGCAGAGA AATCCTACCA GAACAGCCCC 
AGCTCTGACG ACGGCATCAG GCCTCTGCCG GAATATAGCT CAGAAAAACA CAAAAAGCAC 
AAAAAGGAGA AGAAAAAAGT AAAAGACAAA GACCGGGATA GAGACAGGGA GCGAGATAAA 
GACAGAGACA AGAAGAAATC TCACGGCATG AAGCCGGAGA GCTGGTCCAA GTCCCCCATT 
TCTGCTGAGC AGTCTCTCTC CATGGCCAGC AGCACCATCC TCTCGGCCGA GCGACCGGCC 
CGGGCCAGCC CTGAGTTTCT CATCGGGGAA GAAGATGACG ATCTCATGGA TGTTGCTCTA 
ATTGGCAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MED1 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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