MED12 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED12
Entrez Gene ID	106900853
Full Name	mediator complex subunit 12
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014963064.1	XP_014818550.1	mediator of RNA polymerase II transcription subunit 12

Pathways from BioSystems
Gene Ontology

The following MED12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa80210	XM_014963064.1 Latest version!	Calidris pugnax mediator complex subunit 12 (MED12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED12 ( XM_014963064.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014818550.1 Calidris pugnax mediator complex subunit 12 (MED12), mRNA.

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CloneID	OCa80210
Clone ID Related Accession (Same CDS sequence)	XM_014963064.1
Accession Version	XM_014963064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	mediator of RNA polymerase II transcription subunit 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094505.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGCTT TCGGCGATGA GCTGACCGCT CTGAACGTCA AGCAAGGCTT CAATAACCAG 
CCGGCGGTCT CCGGGGACGA GCACGGCAGT GCCAAAAATG TCAACTTCAA CCCAGCGAAG 
ATCAGTTCAA ATTTTAGCAG CATCATCGCA GAAAAGCTGC GCTGCAATAC GCTGCCTGAC 
ACGGGAAGAC GGAAACCCCA GGTGAACCAG AAGGATAACT TCTGGCTGGT GACAGCGCGT 
TCTCAGAGTG CCATAAACAA CTGGTTCACA GATCTGGCTG GGACTAAGCC CCTCACTCAT 
CTGGCTAAGA AGGTGCCCAT TTTTAGCAAA AAGGAAGAGG TCTTTGGTTA TTTGGCGAAA 
TACACCGTTC CAGTAATGAG AGCGGCCTGG CTCATCAAAA TGACTTGCGC CTATTACGCT 
GCCATCACAG AAACCAAGGT GAAAAAGCGT CACGTCATCG ATCCCTTCAT CGAATGGACG 
CAGATCATCA CCAAGTACTT GTCAGAGCAG CTGCAGAAAA TTGCAGAGTT CTACAGGCAG 
CTCCCAGGGC CAGGCTGTGG CTCACCCTCC GGGCCGATGC CGCAGGAGGT TGAACAAGCT 
TTGAAGCAGT GGGACTACAA CGAGAAACTG GCTATGTTCA TGTTCCAGGA TGGCATGCTG 
GACCGGCATG AGTTCCTGAC GTGGGTCCTC GAGTGCTTTG AGAAGATACG GTCAGGAGAA 
GATGAATTTC TGAAAATGCT CCTACCCCTG CTGCTGCGGT ACTCTGGAGA GTTTGTGCAG 
TCTGCCTACC TGTCCAGACG TCTGGCCTAC TTCTGCACCC GCAGGCTGGC CATGCAGCTG 
GACGGTGCTG GGGGGCACCC GCCCCACATC CTCTCTGCCC AGACGGGGAA CCCTCTCCCC 
TCGACTCCCA CCCCTCAGCC AGCTGCAGCA AATCCTCCTC CCAGCCCTTT CAGCGACTTA 
CTGCTGTGCC CCCAGCACCG GCCAGTGGTG TACGGGCTCA GCTGCATCCT CCAGAGTATA 
ATTTTGTGTT GCCCAAGTGC CCTTGTGTGG CATTACTCCC TGACTGACAG CAGGATAAAG 
ACCGGCTCCC CGTTGGACCA CCTGCCTATA GCCCCATCCA ACTTGCCCAT GCCCGGAGGG 
AATTCGGCTT TTACACAGCA GGTTCGAGCA AAGCTGCGTG AAATTGAGCA GCAGATAAAG 
GAGCGTGGCC AGGCCGTGGA GGTTCGCTGG TCATTTGACA AGTGCCAGGA AACCACAGCA 
GGTTTCACTA TTGGCCGTGT CTTGCACACT TTAGAAGTTC TGGACAGTCA CAGCTTTGAA 
AGATCTGACT TCAGCAACTC TTTGGATTCC TTGTATAACA GGATATTTGG GCTGGGTCCA 
ACCAAAGACA GTCACGAGAT CTCCCCAGAT GACGATGCGG TGGTGGCCTT GCTGTGCGAG 
TGGGCTGTCA GCTATAAACG CTCCGGACGC CACAGGGCCA TGGTTGTGGC CAAACTCCTG 
GAGAAGCGTC AAGCAGAGAT AGAGGCTGAG AGATGTGGGG ACTCTGAAGT CGTGGATGAG 
AAGGGCTCCA TCTCTTCAGG CTCTCTCTCG GCAGCCAGCG CTCCTGTCTT TCAGGATGTC 
CTTATGCAGT TCCTTGACAC CCAAGCTCCT ATGCTAACTG ATCCTGGGAA GGAAAATGAG 
AAGGTGGAGT TCTTTAACCT GGTGCTGCTG TTCTGTGAGC TAATCCGACA CGATGTCTTC 
TCTCACAACA TCTACATGTG CACCCTCATC TCCCGAGGTG ACCTTGCCAT GGATTCTCAT 
GGGCCTCGCC CGCCCTCCCC GTTCGATGAC CCTGCTGAGG AGCACGACAG GAAGGAGACG 
GAGGGAAGCA GTGGCATCAA GCTGGAGGAC ACAGGTCTTT CTGAGCCCAT GGACATCGAC 
CACAACTCCA GCATGCTCTT TGATGACATG GAGAAGACAG ACTTCTCGAT GTTTTCTCCT 
CCAATGCATT GTGAATCTAA AGCCAGCCCT TCCCCTGAGA AACCAGATCC TGAAAAGGAA 
GCAAAGCCAC TGCTGAAGGA TAAGTCTGTG GAAGGAATGC TAGCATCCCT GTATGACCAG 
CCTCGGCACA TCCAGTATGC AACACACTTC CCTATTCCTC AGGAGGAATC GTGCAGTCAC 
GAGTGTAACC AACGGTTGGT AGTTCTTTTT GGGGTTGGAA AACAACGGGA CGATGCTCGG 
CATACCATCA AGAAAATAAC CAAAGACATT CTAAAAGTCT TAAACAGAAA GAGCACTGCA 
GAGACGGGTG GAGAAGAAGG CCAGAAGAGG AAAAAGAGCA AGCCAGAAGC ATTCCCAACA 
GCTGAAGATA TCTTTGCAAA GTTCCAGCAC CTTTCTCACT TTGATCAGCA CCAAGTCACA 
TCTCAGGTAT CTCGCAATGT CTTGGAACAG ATCACCAGCT TTGCCTTGGG AATGTCCTAC 
CACCTGCCTC TGGTACAGCA CGTGCAGTTC ATATTTGACT TGATGGAGTA TTCACTCAAT 
ATCAGTGGTC TTATCGACTT TGCCATCCAG TTGCTGAATG AACTGAGCGT GGTGGAGGCA 
GAACTGCTGT TGAAATCCTC CGACCTTGTT GGCAGCTACA CCACCAGCTT GTGCCTGTGC 
ATCGTGGCTG TGCTGCGACA CTACCACTCC TGCCTTATAC TGAACCAGGA CCAGATGGCT 
CAAGTCTTTG AAGGCCTGTG TGGGGTGGTG AAACACGGCA TGAACCGCTC AGATGGCTCC 
TCAGCAGAGC GCTGTATCCT GGCCTATCTC TACGACCTCT ATACCTCCTG CAGTCACCTC 
AAAAGTAAAT TTGGGGAGCT TTTTAGTGAC TTCTGCTCCA AGGTGAAGAA TACAATCTAC 
TGCAATGTTG AGCCATCTGA CTCCAACATG CTATGGGAAC CAGAGTTCAT GATTGACACC 
ATTGAGAATC CATCTGCACA TAACTTTACC TACACCAACC TGGGCAAGAG CCTCAATGAA 
AATCCAGCCA ACCGCTACAG TTTCGTCTGT AATGCACTTA TGCACATCTC CCCAGATGAT 
GATGCGGTGG TGGCCTTGCT GTGCGAGTGG GCTGTCAGCT ATAAACGCTC CGGACGCCAC 
AGGGCCATGG TTGTGGCCAA ACTCCTGGAG AAGCGTCAAG CAGAGATAGA GGCTGAGAGA 
TGTGGGGACT CTGAAGTCGT GGATGAGAAG GGCTCCATCT CTTCAGGCTC TCTCTCGGCA 
GCCAGCGCTC CTGTCTTTCA GGATGTCCTT ATGCAGTTCC TTGACACCCA AGCTCCTATG 
CTAACTGATC CTGGGAAGGA AAATGAGAAG GTGGAGTTCT TTAACCTGGT GCTGCTGTTC 
TGTGAGCTAA TCCGACACGA TGTCTTCTCT CACAACATCT ACATGTGCAC ACTCATCTCC 
CGAGGTGACC TTGCCATGGA TGCTCATGGG CCTCGCCCGC CCTCCCCGTT CGATGACCCT 
GCTGAGGAGC ACGACAGGAA GGAGACGGAG GGAAGCAGTG GCATCAAGCT GGAGGACACA 
GGTCTTTCTG AGCCCATGGA CATCGACCAC AACTCCAGCA TGCTCTTTGA TGACATGGAG 
AAGACAGACT TCTCGATGTT TTCTCCTCCA ATGCATTGTG AATCTAAAGC CAGCCCTTCC 
CCTGAGAAAC CAGATCCTGA AAAGGAAGCA AAGCCACTGC TGAAGGATAA GTCTGTGGAA 
GGAATGCTAG CATCCCTGTA TGACCAGCCT CGGCACATCC AGTATGCAAC ACACTTCCCT 
ATTCCTCAGG AGGAATCGTG CAGTCACGAG TGTAACCAAC GGTTGGTAGT TCTTTTTGGG 
GTTGGAAAAC AACGGGACGA TGCTCGGCAT ACCATCAAGA AAATAACCAA AGACATTCTA 
AAAGTCTTAA ACAGAAAGAG CACTGCAGAG ACGGGTGGAG AAGAAGGCCA GAAGAGGAAA 
AAGAGCAAGC CAGAAGCATT CCCAACAGCT GAAGATATCT TTGCAAAGTT CCAGCACCTT 
TCTCACTTTG ATCAGCACCA AGTCACATCT CAGGTATCTC GCAATGTCTT GGAACAGATC 
ACCAGCTTTG CCTTGGGAAT GTCCTACCAC CTGCCTCTGG TACAGCACGT GCAGTTCATA 
TTTGACTTGA TGGAGTATTC ACTCAATATC AGTGGTCTTA TCGACTTTGC CATCCAGTTG 
CTGAATGAAC TGAGCGTGGT GGAGGCAGAA CTGCTGTTGA AATCCTCCGA CCTTGTTGGC 
AGCTACACCA CCAGCTTGTG CCTGTGCATC GTGGCTGTGC TGCGACACTA CCACTCCTGC 
CTTATACTGA ACCAGGACCA GATGGCTCAA GTCTTTGAAG GCCTGTGTGG GGTGGTGAAA 
CACGGCATGA ACCGCTCAGA TGGCTCCTCA GCAGAGCGCT GTATCCTGGC CTATCTCTAC 
GACCTCTATA CCTCCTGCAG TCACCTCAAA AGTAAATTTG GGGAGCTTTT TAGTGACTTC 
TGCTCCAAGG TGAAGAATAC AATCTACTGC AATGTTGAGC CATCTGACTC CAACATGCTA 
TGGGAACCAG AGTTCATGAT TGACACCATT GAGAATCCAT CTGCACATAA CTTTACCTAC 
ACCAACCTGG GCAAGAGCCT CAATGAGAAT CCAGCCAACC GCTACAGTTT CGTCTGTAAT 
GCACTTATGC ACGTGTGTGT GGGACACCAC GATCCAGACA GGGTGAACGA CATCGCTATC 
CTGTGTGCTG AGCTAACGGG CTACTGCAAG TCTCTGAGCG CCGAGTGGCT GGGGGTGCTC 
AAAGCTTTGT GCTGTTCCTC CAATAACGGG ACCTGTGGCT TCAATGATCT CCTCTGCAAT 
GTTGATGTCA GTGACTTATC TTTCCATGAT TCCCTGGCCA CCTTTGTTGC CATCCTCATT 
GCCCGGCAGT GCTTGCTGCT TGAGGACCTG ATTCGCTGTG CTGCTATTCC CTCACTCCTC 
AATGCTGCCT GCAGTGAACA GGACTCAGAA CCAGGAGCAC GTCTGACCTG CCGGATTTTA 
CTCCATCTGT TTAAGACTCC GCAGCTGAAC CCATGCCAGC AGGATGGCAA CAAACCCACG 
GTGGGGATCC GCTCTTCCTG TGACCGTCAC TTACTGGCAG CGTCCCAGAA CCGCATCGTG 
GACGGAGCGG TCTTTGCAGT GCTGAAGGCT GTCTTCGTTC TGGGAGATGC AGAACTGAAA 
GGCTCTGGCT TTTCCCACCC TGGAGGTGTG GATGACCTGA TGGATGATGA GCTGGGCACC 
AGGAAGGCTG GTGGTCGGGT GGTAACTGTA GAGACAGCCA GCTTGGATAT TTATGCCAAG 
TATGTGCTGA GGAGTATATG TCAACAGGAG TGGGTAGGTG AGCGATGTCT GAAGTCCCTC 
TGTGAAGACA GCAACGACTT GCAGGATCCT GTCCTGAGCA GCACGCAGGC CCAGAGGCTG 
ATGCAGCTGA TCTGTTACCC ACACCGGCTG CTGGACAATG AGGAAGGAGA AAATCCTCAG 
CGGCAGAGGA TTAAACGCAT CTTACAGAAT CTGGACCAGT GGACCATGAG GCAATCCTCG 
CTGGAGCTGC AGCTCATGAT TAAGCAGACA GCCAGCAATG AGATGAATTC CTTGTTAGAA 
AATATAGCCA AGGCCACTAT TGAGGTGTTC CAGCAGTCAG CAGAGACCAG CTCTGCTAGC 
TCTGCTGGCA ATGGAGTCAA CAATATCAGT AGCTCAGCAA GTGCTGCACC TGCCAGCAAC 
AAATCCAAAC CCATCCTCAG CTCCCTGGAG AGATCAGGAG TGTGGCTGGT GGCCCCTCTG 
ATTGCCAAGC TTCCAACATC AGTGCAGGGC CATGTGCTGA AAGCTGCTGG AGAAGAACTG 
GAGAAAGGGC AACATCTGGG GTCATCGTCC CGCAAAGAGC GGGACCGCCA GAAGCAGAAG 
AGCATGTCCC TCCTGAGCCA GCAGCCATTT CTGTCGCTGG TGCTAACATG CTTGAAAGGA 
CAGGATGAGC AGCGGGAAGG CCTCCTCACC TCTCTGTACA GTCAGGTCCA ACAGATTGTT 
ACGAATTGGC GGGAAGATCA GTACCAGGAT GACTGCAAAG CCAAGCAGCT GATGCATGAG 
GCCCTGAAAC TACGGCTGAA TTTGGTGGGG GGAATGTTTG ACACAGTTCA ACGCAGTACA 
CAGCAGACAA CTGAGTGGGC TGTCCTTCTC CTGGACATCA TCAGCAGTGG CACCGTGGAC 
ATGCAGTCAA ACAATGAGCT CTTCACCACC GTGTTGGACA TGCTGAGCGT TCTCATCAAC 
GGCACCCTGG CTGCTGATAT GTCCAGCATT TCTCAGGGCA GCATGGAGGA GAACAAGCGG 
GCCTACATGA ATCTTGTCAA GAAACTCAGG AAAGAGCTGG GGGACCGGCA ATCTGACAGC 
CTGGAGAAGG TGCGACAGCT ACTGCCACTT CCCAAGCAGA CCCGAGATGT CATCACCTGT 
GAACCTCAGG GATCCCTTAT TGACACCAAA GGCAATAAAA TAGCTGGATT TGACTCCATC 
TTCAAGAAGG AGGGTTTGCA AGTCTCTACA AAGCAGAAGA TTTCCCCTTG GGATCTGTTT 
GAGGGCTTAA AGCACTCGGC CCCGCTCTCC TGGGGGTGGT TTGGGACGGT CCGGGTGGAT 
CGCAAGGTGT CTAGGTTTGA GGAGCAGCAG AGGCTTCTTC TGTACCACAC GCACTTGAAA 
CCCAAGCCCC GCAGTTACTA CCTGGAGCCG TTGCCTCTGC CCCCTGAAGA GGAAGAGCCT 
CCTACGCCTG TGGCTTTAGA GCCAGAGAAG AAAGCTGCAG AGCCAGCGAA GGCTGATAAA 
ACAAGCTCCA ATCCTGCCAC TTCTACTGAA GAACGCAAGA AGAAACAGAG CAAAACCAAG 
AAACGCAACC AATCTGCCAG CAAAACTGAG GATTTCGTGT TGGGCCCTAG CCGAGGGGTT 
TCCTATGGGG TTGGTATGCC TACAGATCTC TTGCATCACC AGTCAGGAAG CACTATGTCG 
AGGTTGGCAT ACGGGCAATC ACCAGTGGGT CTCTATGCCC AGAATCAGCC TCTTCCAGCA 
GGTGGCCCTC GCCTGGACAC ATCCTACAGA CCCGTGCGCA TGCCGTTGGG GAAACTTGTT 
CAGAGCCGTC CTCCCTACAG CGGTGTGCTG CCCCCAGGGA TGGGCAGCAT GATGGGCATC 
GACCCCTCCT ACAAGCCAGC AGTGTACAGA CAGCAGCCCC CGGTGTCCCA GGGACAGATC 
CTGAGGCAGC AGCTCCAAGC AAAGTTGCAG GGCCAAGGCA TAATGGGACA GCAGCCAGTG 
CGCCAGATGG CTCCAACCCC GTCCTACGGA GCACTGCAGC CCTCACAGGG TTACACACCT 
TACGTCTCCC ACATAGGCCT TCAGCAGCAC CCCTCCCAGT CAGGCACGAT GGTACCTCCT 
ACCTATTCTG GCCAGCCCTA CCAGAATTCC CACCCCAGCT CTAATCCTGC CCTGGTGGAT 
CCTGTTAGAC AGATGCAGCA GAGACCAAGC GGCTACGTAC ACCAGCAGGC CCCTGGCTAC 
GGGCACACTT TGGGCAACAC ACAGAGATTT CCTCACCAGT CAATACAGCA GACCCCCATG 
ATGAGTGGGA TGAACCATTT GGGTCCACAA GGAGTTCCCT CAGGAATTCG ACCCAGCCAG 
ATATTGCCTG ACCAGCAGCA GCAGCAGTAC CTGAGGCAGC AGCAGCAACA GCAGCAGCAG 
ATGCTGAGGC AGCAGCAACA ACAACAACAA CAGCAGCAAC AGCAGCAGCA ACAACAGCAA 
CAGCAGCAGC AGCAGCCCCA GCCGCCGCAG CCCCAGCCGC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCGGGGC TGGGGATGCA GGCTCTCCCC 
CCCCAGCAGC CCATCTTCCA GCGTCAGGGC CTTCAGCAGA CACAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CCCAGACCCA GCAGAACAAC 
AACCCCTTCG GACGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014818550.1
CDS	1..8061
Translation	MAAFGDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINNWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFIEWTQIITKYLSEQLQKIAEFYRQLPGPGCGSPSGPMPQEVEQALKQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRSGEDEFLKMLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLAMQLDGAGGHPPHILSAQTGNPLPSTPTPQPAAANPPPSPFSDLLLCPQHRPVVYGLSCILQSIILCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPGGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQETTAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLYNRIFGLGPTKDSHEISPDDDAVVALLCEWAVSYKRSGRHRAMVVAKLLEKRQAEIEAERCGDSEVVDEKGSISSGSLSAASAPVFQDVLMQFLDTQAPMLTDPGKENEKVEFFNLVLLFCELIRHDVFSHNIYMCTLISRGDLAMDSHGPRPPSPFDDPAEEHDRKETEGSSGIKLEDTGLSEPMDIDHNSSMLFDDMEKTDFSMFSPPMHCESKASPSPEKPDPEKEAKPLLKDKSVEGMLASLYDQPRHIQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHTIKKITKDILKVLNRKSTAETGGEEGQKRKKSKPEAFPTAEDIFAKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPEFMIDTIENPSAHNFTYTNLGKSLNENPANRYSFVCNALMHISPDDDAVVALLCEWAVSYKRSGRHRAMVVAKLLEKRQAEIEAERCGDSEVVDEKGSISSGSLSAASAPVFQDVLMQFLDTQAPMLTDPGKENEKVEFFNLVLLFCELIRHDVFSHNIYMCTLISRGDLAMDAHGPRPPSPFDDPAEEHDRKETEGSSGIKLEDTGLSEPMDIDHNSSMLFDDMEKTDFSMFSPPMHCESKASPSPEKPDPEKEAKPLLKDKSVEGMLASLYDQPRHIQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHTIKKITKDILKVLNRKSTAETGGEEGQKRKKSKPEAFPTAEDIFAKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLNISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHSCLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSDSNMLWEPEFMIDTIENPSAHNFTYTNLGKSLNENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQQDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFSHPGGVDDLMDDELGTRKAGGRVVTVETASLDIYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSTQAQRLMQLICYPHRLLDNEEGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTASNEMNSLLENIAKATIEVFQQSAETSSASSAGNGVNNISSSASAAPASNKSKPILSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKHSAPLSWGWFGTVRVDRKVSRFEEQQRLLLYHTHLKPKPRSYYLEPLPLPPEEEEPPTPVALEPEKKAAEPAKADKTSSNPATSTEERKKKQSKTKKRNQSASKTEDFVLGPSRGVSYGVGMPTDLLHHQSGSTMSRLAYGQSPVGLYAQNQPLPAGGPRLDTSYRPVRMPLGKLVQSRPPYSGVLPPGMGSMMGIDPSYKPAVYRQQPPVSQGQILRQQLQAKLQGQGIMGQQPVRQMAPTPSYGALQPSQGYTPYVSHIGLQQHPSQSGTMVPPTYSGQPYQNSHPSSNPALVDPVRQMQQRPSGYVHQQAPGYGHTLGNTQRFPHQSIQQTPMMSGMNHLGPQGVPSGIRPSQILPDQQQQQYLRQQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQPQPPQPQPQQQPQVSTVPQPQAQGQPPGLGMQALPPQQPIFQRQGLQQTQQQQQTAALVRQLQQQLSNTQTQQNNNPFGRY

Target ORF information:

RefSeq Version	XM_014963064.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax mediator complex subunit 12 (MED12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014963064.1

ORF Insert Sequence:

ATGGCGGCTT TCGGCGATGA GCTGACCGCT CTGAACGTCA AGCAAGGCTT CAATAACCAG 
CCGGCGGTCT CCGGGGACGA GCACGGCAGT GCCAAAAATG TCAACTTCAA CCCAGCGAAG 
ATCAGTTCAA ATTTTAGCAG CATCATCGCA GAAAAGCTGC GCTGCAATAC GCTGCCTGAC 
ACGGGAAGAC GGAAACCCCA GGTGAACCAG AAGGATAACT TCTGGCTGGT GACAGCGCGT 
TCTCAGAGTG CCATAAACAA CTGGTTCACA GATCTGGCTG GGACTAAGCC CCTCACTCAT 
CTGGCTAAGA AGGTGCCCAT TTTTAGCAAA AAGGAAGAGG TCTTTGGTTA TTTGGCGAAA 
TACACCGTTC CAGTAATGAG AGCGGCCTGG CTCATCAAAA TGACTTGCGC CTATTACGCT 
GCCATCACAG AAACCAAGGT GAAAAAGCGT CACGTCATCG ATCCCTTCAT CGAATGGACG 
CAGATCATCA CCAAGTACTT GTCAGAGCAG CTGCAGAAAA TTGCAGAGTT CTACAGGCAG 
CTCCCAGGGC CAGGCTGTGG CTCACCCTCC GGGCCGATGC CGCAGGAGGT TGAACAAGCT 
TTGAAGCAGT GGGACTACAA CGAGAAACTG GCTATGTTCA TGTTCCAGGA TGGCATGCTG 
GACCGGCATG AGTTCCTGAC GTGGGTCCTC GAGTGCTTTG AGAAGATACG GTCAGGAGAA 
GATGAATTTC TGAAAATGCT CCTACCCCTG CTGCTGCGGT ACTCTGGAGA GTTTGTGCAG 
TCTGCCTACC TGTCCAGACG TCTGGCCTAC TTCTGCACCC GCAGGCTGGC CATGCAGCTG 
GACGGTGCTG GGGGGCACCC GCCCCACATC CTCTCTGCCC AGACGGGGAA CCCTCTCCCC 
TCGACTCCCA CCCCTCAGCC AGCTGCAGCA AATCCTCCTC CCAGCCCTTT CAGCGACTTA 
CTGCTGTGCC CCCAGCACCG GCCAGTGGTG TACGGGCTCA GCTGCATCCT CCAGAGTATA 
ATTTTGTGTT GCCCAAGTGC CCTTGTGTGG CATTACTCCC TGACTGACAG CAGGATAAAG 
ACCGGCTCCC CGTTGGACCA CCTGCCTATA GCCCCATCCA ACTTGCCCAT GCCCGGAGGG 
AATTCGGCTT TTACACAGCA GGTTCGAGCA AAGCTGCGTG AAATTGAGCA GCAGATAAAG 
GAGCGTGGCC AGGCCGTGGA GGTTCGCTGG TCATTTGACA AGTGCCAGGA AACCACAGCA 
GGTTTCACTA TTGGCCGTGT CTTGCACACT TTAGAAGTTC TGGACAGTCA CAGCTTTGAA 
AGATCTGACT TCAGCAACTC TTTGGATTCC TTGTATAACA GGATATTTGG GCTGGGTCCA 
ACCAAAGACA GTCACGAGAT CTCCCCAGAT GACGATGCGG TGGTGGCCTT GCTGTGCGAG 
TGGGCTGTCA GCTATAAACG CTCCGGACGC CACAGGGCCA TGGTTGTGGC CAAACTCCTG 
GAGAAGCGTC AAGCAGAGAT AGAGGCTGAG AGATGTGGGG ACTCTGAAGT CGTGGATGAG 
AAGGGCTCCA TCTCTTCAGG CTCTCTCTCG GCAGCCAGCG CTCCTGTCTT TCAGGATGTC 
CTTATGCAGT TCCTTGACAC CCAAGCTCCT ATGCTAACTG ATCCTGGGAA GGAAAATGAG 
AAGGTGGAGT TCTTTAACCT GGTGCTGCTG TTCTGTGAGC TAATCCGACA CGATGTCTTC 
TCTCACAACA TCTACATGTG CACCCTCATC TCCCGAGGTG ACCTTGCCAT GGATTCTCAT 
GGGCCTCGCC CGCCCTCCCC GTTCGATGAC CCTGCTGAGG AGCACGACAG GAAGGAGACG 
GAGGGAAGCA GTGGCATCAA GCTGGAGGAC ACAGGTCTTT CTGAGCCCAT GGACATCGAC 
CACAACTCCA GCATGCTCTT TGATGACATG GAGAAGACAG ACTTCTCGAT GTTTTCTCCT 
CCAATGCATT GTGAATCTAA AGCCAGCCCT TCCCCTGAGA AACCAGATCC TGAAAAGGAA 
GCAAAGCCAC TGCTGAAGGA TAAGTCTGTG GAAGGAATGC TAGCATCCCT GTATGACCAG 
CCTCGGCACA TCCAGTATGC AACACACTTC CCTATTCCTC AGGAGGAATC GTGCAGTCAC 
GAGTGTAACC AACGGTTGGT AGTTCTTTTT GGGGTTGGAA AACAACGGGA CGATGCTCGG 
CATACCATCA AGAAAATAAC CAAAGACATT CTAAAAGTCT TAAACAGAAA GAGCACTGCA 
GAGACGGGTG GAGAAGAAGG CCAGAAGAGG AAAAAGAGCA AGCCAGAAGC ATTCCCAACA 
GCTGAAGATA TCTTTGCAAA GTTCCAGCAC CTTTCTCACT TTGATCAGCA CCAAGTCACA 
TCTCAGGTAT CTCGCAATGT CTTGGAACAG ATCACCAGCT TTGCCTTGGG AATGTCCTAC 
CACCTGCCTC TGGTACAGCA CGTGCAGTTC ATATTTGACT TGATGGAGTA TTCACTCAAT 
ATCAGTGGTC TTATCGACTT TGCCATCCAG TTGCTGAATG AACTGAGCGT GGTGGAGGCA 
GAACTGCTGT TGAAATCCTC CGACCTTGTT GGCAGCTACA CCACCAGCTT GTGCCTGTGC 
ATCGTGGCTG TGCTGCGACA CTACCACTCC TGCCTTATAC TGAACCAGGA CCAGATGGCT 
CAAGTCTTTG AAGGCCTGTG TGGGGTGGTG AAACACGGCA TGAACCGCTC AGATGGCTCC 
TCAGCAGAGC GCTGTATCCT GGCCTATCTC TACGACCTCT ATACCTCCTG CAGTCACCTC 
AAAAGTAAAT TTGGGGAGCT TTTTAGTGAC TTCTGCTCCA AGGTGAAGAA TACAATCTAC 
TGCAATGTTG AGCCATCTGA CTCCAACATG CTATGGGAAC CAGAGTTCAT GATTGACACC 
ATTGAGAATC CATCTGCACA TAACTTTACC TACACCAACC TGGGCAAGAG CCTCAATGAA 
AATCCAGCCA ACCGCTACAG TTTCGTCTGT AATGCACTTA TGCACATCTC CCCAGATGAT 
GATGCGGTGG TGGCCTTGCT GTGCGAGTGG GCTGTCAGCT ATAAACGCTC CGGACGCCAC 
AGGGCCATGG TTGTGGCCAA ACTCCTGGAG AAGCGTCAAG CAGAGATAGA GGCTGAGAGA 
TGTGGGGACT CTGAAGTCGT GGATGAGAAG GGCTCCATCT CTTCAGGCTC TCTCTCGGCA 
GCCAGCGCTC CTGTCTTTCA GGATGTCCTT ATGCAGTTCC TTGACACCCA AGCTCCTATG 
CTAACTGATC CTGGGAAGGA AAATGAGAAG GTGGAGTTCT TTAACCTGGT GCTGCTGTTC 
TGTGAGCTAA TCCGACACGA TGTCTTCTCT CACAACATCT ACATGTGCAC ACTCATCTCC 
CGAGGTGACC TTGCCATGGA TGCTCATGGG CCTCGCCCGC CCTCCCCGTT CGATGACCCT 
GCTGAGGAGC ACGACAGGAA GGAGACGGAG GGAAGCAGTG GCATCAAGCT GGAGGACACA 
GGTCTTTCTG AGCCCATGGA CATCGACCAC AACTCCAGCA TGCTCTTTGA TGACATGGAG 
AAGACAGACT TCTCGATGTT TTCTCCTCCA ATGCATTGTG AATCTAAAGC CAGCCCTTCC 
CCTGAGAAAC CAGATCCTGA AAAGGAAGCA AAGCCACTGC TGAAGGATAA GTCTGTGGAA 
GGAATGCTAG CATCCCTGTA TGACCAGCCT CGGCACATCC AGTATGCAAC ACACTTCCCT 
ATTCCTCAGG AGGAATCGTG CAGTCACGAG TGTAACCAAC GGTTGGTAGT TCTTTTTGGG 
GTTGGAAAAC AACGGGACGA TGCTCGGCAT ACCATCAAGA AAATAACCAA AGACATTCTA 
AAAGTCTTAA ACAGAAAGAG CACTGCAGAG ACGGGTGGAG AAGAAGGCCA GAAGAGGAAA 
AAGAGCAAGC CAGAAGCATT CCCAACAGCT GAAGATATCT TTGCAAAGTT CCAGCACCTT 
TCTCACTTTG ATCAGCACCA AGTCACATCT CAGGTATCTC GCAATGTCTT GGAACAGATC 
ACCAGCTTTG CCTTGGGAAT GTCCTACCAC CTGCCTCTGG TACAGCACGT GCAGTTCATA 
TTTGACTTGA TGGAGTATTC ACTCAATATC AGTGGTCTTA TCGACTTTGC CATCCAGTTG 
CTGAATGAAC TGAGCGTGGT GGAGGCAGAA CTGCTGTTGA AATCCTCCGA CCTTGTTGGC 
AGCTACACCA CCAGCTTGTG CCTGTGCATC GTGGCTGTGC TGCGACACTA CCACTCCTGC 
CTTATACTGA ACCAGGACCA GATGGCTCAA GTCTTTGAAG GCCTGTGTGG GGTGGTGAAA 
CACGGCATGA ACCGCTCAGA TGGCTCCTCA GCAGAGCGCT GTATCCTGGC CTATCTCTAC 
GACCTCTATA CCTCCTGCAG TCACCTCAAA AGTAAATTTG GGGAGCTTTT TAGTGACTTC 
TGCTCCAAGG TGAAGAATAC AATCTACTGC AATGTTGAGC CATCTGACTC CAACATGCTA 
TGGGAACCAG AGTTCATGAT TGACACCATT GAGAATCCAT CTGCACATAA CTTTACCTAC 
ACCAACCTGG GCAAGAGCCT CAATGAGAAT CCAGCCAACC GCTACAGTTT CGTCTGTAAT 
GCACTTATGC ACGTGTGTGT GGGACACCAC GATCCAGACA GGGTGAACGA CATCGCTATC 
CTGTGTGCTG AGCTAACGGG CTACTGCAAG TCTCTGAGCG CCGAGTGGCT GGGGGTGCTC 
AAAGCTTTGT GCTGTTCCTC CAATAACGGG ACCTGTGGCT TCAATGATCT CCTCTGCAAT 
GTTGATGTCA GTGACTTATC TTTCCATGAT TCCCTGGCCA CCTTTGTTGC CATCCTCATT 
GCCCGGCAGT GCTTGCTGCT TGAGGACCTG ATTCGCTGTG CTGCTATTCC CTCACTCCTC 
AATGCTGCCT GCAGTGAACA GGACTCAGAA CCAGGAGCAC GTCTGACCTG CCGGATTTTA 
CTCCATCTGT TTAAGACTCC GCAGCTGAAC CCATGCCAGC AGGATGGCAA CAAACCCACG 
GTGGGGATCC GCTCTTCCTG TGACCGTCAC TTACTGGCAG CGTCCCAGAA CCGCATCGTG 
GACGGAGCGG TCTTTGCAGT GCTGAAGGCT GTCTTCGTTC TGGGAGATGC AGAACTGAAA 
GGCTCTGGCT TTTCCCACCC TGGAGGTGTG GATGACCTGA TGGATGATGA GCTGGGCACC 
AGGAAGGCTG GTGGTCGGGT GGTAACTGTA GAGACAGCCA GCTTGGATAT TTATGCCAAG 
TATGTGCTGA GGAGTATATG TCAACAGGAG TGGGTAGGTG AGCGATGTCT GAAGTCCCTC 
TGTGAAGACA GCAACGACTT GCAGGATCCT GTCCTGAGCA GCACGCAGGC CCAGAGGCTG 
ATGCAGCTGA TCTGTTACCC ACACCGGCTG CTGGACAATG AGGAAGGAGA AAATCCTCAG 
CGGCAGAGGA TTAAACGCAT CTTACAGAAT CTGGACCAGT GGACCATGAG GCAATCCTCG 
CTGGAGCTGC AGCTCATGAT TAAGCAGACA GCCAGCAATG AGATGAATTC CTTGTTAGAA 
AATATAGCCA AGGCCACTAT TGAGGTGTTC CAGCAGTCAG CAGAGACCAG CTCTGCTAGC 
TCTGCTGGCA ATGGAGTCAA CAATATCAGT AGCTCAGCAA GTGCTGCACC TGCCAGCAAC 
AAATCCAAAC CCATCCTCAG CTCCCTGGAG AGATCAGGAG TGTGGCTGGT GGCCCCTCTG 
ATTGCCAAGC TTCCAACATC AGTGCAGGGC CATGTGCTGA AAGCTGCTGG AGAAGAACTG 
GAGAAAGGGC AACATCTGGG GTCATCGTCC CGCAAAGAGC GGGACCGCCA GAAGCAGAAG 
AGCATGTCCC TCCTGAGCCA GCAGCCATTT CTGTCGCTGG TGCTAACATG CTTGAAAGGA 
CAGGATGAGC AGCGGGAAGG CCTCCTCACC TCTCTGTACA GTCAGGTCCA ACAGATTGTT 
ACGAATTGGC GGGAAGATCA GTACCAGGAT GACTGCAAAG CCAAGCAGCT GATGCATGAG 
GCCCTGAAAC TACGGCTGAA TTTGGTGGGG GGAATGTTTG ACACAGTTCA ACGCAGTACA 
CAGCAGACAA CTGAGTGGGC TGTCCTTCTC CTGGACATCA TCAGCAGTGG CACCGTGGAC 
ATGCAGTCAA ACAATGAGCT CTTCACCACC GTGTTGGACA TGCTGAGCGT TCTCATCAAC 
GGCACCCTGG CTGCTGATAT GTCCAGCATT TCTCAGGGCA GCATGGAGGA GAACAAGCGG 
GCCTACATGA ATCTTGTCAA GAAACTCAGG AAAGAGCTGG GGGACCGGCA ATCTGACAGC 
CTGGAGAAGG TGCGACAGCT ACTGCCACTT CCCAAGCAGA CCCGAGATGT CATCACCTGT 
GAACCTCAGG GATCCCTTAT TGACACCAAA GGCAATAAAA TAGCTGGATT TGACTCCATC 
TTCAAGAAGG AGGGTTTGCA AGTCTCTACA AAGCAGAAGA TTTCCCCTTG GGATCTGTTT 
GAGGGCTTAA AGCACTCGGC CCCGCTCTCC TGGGGGTGGT TTGGGACGGT CCGGGTGGAT 
CGCAAGGTGT CTAGGTTTGA GGAGCAGCAG AGGCTTCTTC TGTACCACAC GCACTTGAAA 
CCCAAGCCCC GCAGTTACTA CCTGGAGCCG TTGCCTCTGC CCCCTGAAGA GGAAGAGCCT 
CCTACGCCTG TGGCTTTAGA GCCAGAGAAG AAAGCTGCAG AGCCAGCGAA GGCTGATAAA 
ACAAGCTCCA ATCCTGCCAC TTCTACTGAA GAACGCAAGA AGAAACAGAG CAAAACCAAG 
AAACGCAACC AATCTGCCAG CAAAACTGAG GATTTCGTGT TGGGCCCTAG CCGAGGGGTT 
TCCTATGGGG TTGGTATGCC TACAGATCTC TTGCATCACC AGTCAGGAAG CACTATGTCG 
AGGTTGGCAT ACGGGCAATC ACCAGTGGGT CTCTATGCCC AGAATCAGCC TCTTCCAGCA 
GGTGGCCCTC GCCTGGACAC ATCCTACAGA CCCGTGCGCA TGCCGTTGGG GAAACTTGTT 
CAGAGCCGTC CTCCCTACAG CGGTGTGCTG CCCCCAGGGA TGGGCAGCAT GATGGGCATC 
GACCCCTCCT ACAAGCCAGC AGTGTACAGA CAGCAGCCCC CGGTGTCCCA GGGACAGATC 
CTGAGGCAGC AGCTCCAAGC AAAGTTGCAG GGCCAAGGCA TAATGGGACA GCAGCCAGTG 
CGCCAGATGG CTCCAACCCC GTCCTACGGA GCACTGCAGC CCTCACAGGG TTACACACCT 
TACGTCTCCC ACATAGGCCT TCAGCAGCAC CCCTCCCAGT CAGGCACGAT GGTACCTCCT 
ACCTATTCTG GCCAGCCCTA CCAGAATTCC CACCCCAGCT CTAATCCTGC CCTGGTGGAT 
CCTGTTAGAC AGATGCAGCA GAGACCAAGC GGCTACGTAC ACCAGCAGGC CCCTGGCTAC 
GGGCACACTT TGGGCAACAC ACAGAGATTT CCTCACCAGT CAATACAGCA GACCCCCATG 
ATGAGTGGGA TGAACCATTT GGGTCCACAA GGAGTTCCCT CAGGAATTCG ACCCAGCCAG 
ATATTGCCTG ACCAGCAGCA GCAGCAGTAC CTGAGGCAGC AGCAGCAACA GCAGCAGCAG 
ATGCTGAGGC AGCAGCAACA ACAACAACAA CAGCAGCAAC AGCAGCAGCA ACAACAGCAA 
CAGCAGCAGC AGCAGCCCCA GCCGCCGCAG CCCCAGCCGC AGCAGCAGCC CCAGGTCTCC 
ACAGTGCCTC AGCCACAGGC GCAGGGCCAG CCCCCGGGGC TGGGGATGCA GGCTCTCCCC 
CCCCAGCAGC CCATCTTCCA GCGTCAGGGC CTTCAGCAGA CACAGCAGCA GCAGCAAACA 
GCTGCTCTGG TTCGACAGCT TCAACAGCAG CTTTCCAATA CCCAGACCCA GCAGAACAAC 
AACCCCTTCG GACGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MED12 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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