SEMA3D cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol SEMA3D

Entrez Gene ID 106901273

Full Name semaphorin 3D

Gene Type protein-coding

Organism Calidris pugnax(ruff)

mRNA	Protein	Name
XM_014963794.1	XP_014819280.1	semaphorin-3D
XM_014963796.1	XP_014819282.1	semaphorin-3D
XM_014963798.1	XP_014819284.1	semaphorin-3D
XM_014963793.1	XP_014819279.1	semaphorin-3D
XM_014963795.1	XP_014819281.1	semaphorin-3D
XM_014963792.1	XP_014819278.1	semaphorin-3D

Pathways from BioSystems
Gene Ontology

The following SEMA3D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA3D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa80738	XM_014963792.1 Latest version!	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa80738	XM_014963795.1 Latest version!	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa80738	XM_014963798.1 Latest version!	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa80738	XM_014963793.1 Latest version!	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa80738	XM_014963794.1 Latest version!	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa80738	XM_014963796.1 Latest version!	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA3D ( XM_014963794.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014819280.1 Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCa80738
Clone ID Related Accession (Same CDS sequence)	XM_014963794.1 , XM_014963796.1 , XM_014963798.1 , XM_014963793.1 , XM_014963795.1 , XM_014963792.1
Accession Version	XM_014963794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	semaphorin-3D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094506.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014819280.1
CDS	207..2492
Translation	MRASQVPHACSLLSLAMLFLPVTGTSKQNIPRLKLSYKDLLLSNSCIPFLGSTEGLDFRTLLLDEERGRLLIGAKDHIFLLNLVDLNKNVKKIYWPAAKEKVELCKLAGKDAHTECANFIRVLQPYNRTHVYVCGTGAFHPLCGYIELGTHKEETVFRLDTQNLESGRLKCPFDPQLPFASVMADEYLYAGTASDFLGKDTALTRSLGPSHDHHYIRTDISEHYWLTGAKFVGTFPIPDTYNPDDDKIYFFFREISQDSGTSDKTILSRVGRVCKNDMGGQRSLINKWTTFLKARLVCSIPGPEGADTHFDELQDIFLLSTRDERNPLVYGVFTTTSSVFKGSAVCVYSMADIRAVFNGPYAHKESVDHRWVQYEGRIPYPRPGTCPSKTYDPLIKSTRDFPDEVISFIKRHPLMYKSVYPVTGGPVFTRINVDYRLTQIVVDHVMAEDGQYDVIFLGTDIGTVLKVVSITKEKWTKEEVVLEELQIFKHPSFISTMEISQKQQQLYIGSRDGLVQLSLHRCHTYGKACADCCLARDPYCAWDGNSCSRYAPTSKRRARRQDVKYGDPVAQCWDVEDSISHETADEKVIFGIEFNSTFLECIPKSQQASIRWYIQRSGEEHREELKADERIIKTEHGLLIRSLQRKDAGAYFCKAQEHTFVHTIVKLNLNVIENGQMESTQKTEDEEGRVRDLLTESRLRYKDYIQLVSSPSFSLDEYCEQMWHREKRRQRNKGGAKWKHVQEMKKKRNRRHHEPARPPST

Target ORF information:

RefSeq Version	XM_014963794.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014963794.1

ORF Insert Sequence:

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3D ( XM_014963796.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014819282.1 Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCa80738
Clone ID Related Accession (Same CDS sequence)	XM_014963794.1 , XM_014963796.1 , XM_014963798.1 , XM_014963793.1 , XM_014963795.1 , XM_014963792.1
Accession Version	XM_014963796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	semaphorin-3D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094506.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014819282.1
CDS	186..2471
Translation	MRASQVPHACSLLSLAMLFLPVTGTSKQNIPRLKLSYKDLLLSNSCIPFLGSTEGLDFRTLLLDEERGRLLIGAKDHIFLLNLVDLNKNVKKIYWPAAKEKVELCKLAGKDAHTECANFIRVLQPYNRTHVYVCGTGAFHPLCGYIELGTHKEETVFRLDTQNLESGRLKCPFDPQLPFASVMADEYLYAGTASDFLGKDTALTRSLGPSHDHHYIRTDISEHYWLTGAKFVGTFPIPDTYNPDDDKIYFFFREISQDSGTSDKTILSRVGRVCKNDMGGQRSLINKWTTFLKARLVCSIPGPEGADTHFDELQDIFLLSTRDERNPLVYGVFTTTSSVFKGSAVCVYSMADIRAVFNGPYAHKESVDHRWVQYEGRIPYPRPGTCPSKTYDPLIKSTRDFPDEVISFIKRHPLMYKSVYPVTGGPVFTRINVDYRLTQIVVDHVMAEDGQYDVIFLGTDIGTVLKVVSITKEKWTKEEVVLEELQIFKHPSFISTMEISQKQQQLYIGSRDGLVQLSLHRCHTYGKACADCCLARDPYCAWDGNSCSRYAPTSKRRARRQDVKYGDPVAQCWDVEDSISHETADEKVIFGIEFNSTFLECIPKSQQASIRWYIQRSGEEHREELKADERIIKTEHGLLIRSLQRKDAGAYFCKAQEHTFVHTIVKLNLNVIENGQMESTQKTEDEEGRVRDLLTESRLRYKDYIQLVSSPSFSLDEYCEQMWHREKRRQRNKGGAKWKHVQEMKKKRNRRHHEPARPPST

Target ORF information:

RefSeq Version	XM_014963796.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014963796.1

ORF Insert Sequence:

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3D ( XM_014963798.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014819284.1 Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCa80738
Clone ID Related Accession (Same CDS sequence)	XM_014963794.1 , XM_014963796.1 , XM_014963798.1 , XM_014963793.1 , XM_014963795.1 , XM_014963792.1
Accession Version	XM_014963798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	semaphorin-3D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094506.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014819284.1
CDS	98..2383
Translation	MRASQVPHACSLLSLAMLFLPVTGTSKQNIPRLKLSYKDLLLSNSCIPFLGSTEGLDFRTLLLDEERGRLLIGAKDHIFLLNLVDLNKNVKKIYWPAAKEKVELCKLAGKDAHTECANFIRVLQPYNRTHVYVCGTGAFHPLCGYIELGTHKEETVFRLDTQNLESGRLKCPFDPQLPFASVMADEYLYAGTASDFLGKDTALTRSLGPSHDHHYIRTDISEHYWLTGAKFVGTFPIPDTYNPDDDKIYFFFREISQDSGTSDKTILSRVGRVCKNDMGGQRSLINKWTTFLKARLVCSIPGPEGADTHFDELQDIFLLSTRDERNPLVYGVFTTTSSVFKGSAVCVYSMADIRAVFNGPYAHKESVDHRWVQYEGRIPYPRPGTCPSKTYDPLIKSTRDFPDEVISFIKRHPLMYKSVYPVTGGPVFTRINVDYRLTQIVVDHVMAEDGQYDVIFLGTDIGTVLKVVSITKEKWTKEEVVLEELQIFKHPSFISTMEISQKQQQLYIGSRDGLVQLSLHRCHTYGKACADCCLARDPYCAWDGNSCSRYAPTSKRRARRQDVKYGDPVAQCWDVEDSISHETADEKVIFGIEFNSTFLECIPKSQQASIRWYIQRSGEEHREELKADERIIKTEHGLLIRSLQRKDAGAYFCKAQEHTFVHTIVKLNLNVIENGQMESTQKTEDEEGRVRDLLTESRLRYKDYIQLVSSPSFSLDEYCEQMWHREKRRQRNKGGAKWKHVQEMKKKRNRRHHEPARPPST

Target ORF information:

RefSeq Version	XM_014963798.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014963798.1

ORF Insert Sequence:

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3D ( XM_014963793.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014819279.1 Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa80738
Clone ID Related Accession (Same CDS sequence)	XM_014963794.1 , XM_014963796.1 , XM_014963798.1 , XM_014963793.1 , XM_014963795.1 , XM_014963792.1
Accession Version	XM_014963793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	semaphorin-3D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094506.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014819279.1
CDS	220..2505
Translation	MRASQVPHACSLLSLAMLFLPVTGTSKQNIPRLKLSYKDLLLSNSCIPFLGSTEGLDFRTLLLDEERGRLLIGAKDHIFLLNLVDLNKNVKKIYWPAAKEKVELCKLAGKDAHTECANFIRVLQPYNRTHVYVCGTGAFHPLCGYIELGTHKEETVFRLDTQNLESGRLKCPFDPQLPFASVMADEYLYAGTASDFLGKDTALTRSLGPSHDHHYIRTDISEHYWLTGAKFVGTFPIPDTYNPDDDKIYFFFREISQDSGTSDKTILSRVGRVCKNDMGGQRSLINKWTTFLKARLVCSIPGPEGADTHFDELQDIFLLSTRDERNPLVYGVFTTTSSVFKGSAVCVYSMADIRAVFNGPYAHKESVDHRWVQYEGRIPYPRPGTCPSKTYDPLIKSTRDFPDEVISFIKRHPLMYKSVYPVTGGPVFTRINVDYRLTQIVVDHVMAEDGQYDVIFLGTDIGTVLKVVSITKEKWTKEEVVLEELQIFKHPSFISTMEISQKQQQLYIGSRDGLVQLSLHRCHTYGKACADCCLARDPYCAWDGNSCSRYAPTSKRRARRQDVKYGDPVAQCWDVEDSISHETADEKVIFGIEFNSTFLECIPKSQQASIRWYIQRSGEEHREELKADERIIKTEHGLLIRSLQRKDAGAYFCKAQEHTFVHTIVKLNLNVIENGQMESTQKTEDEEGRVRDLLTESRLRYKDYIQLVSSPSFSLDEYCEQMWHREKRRQRNKGGAKWKHVQEMKKKRNRRHHEPARPPST

Target ORF information:

RefSeq Version	XM_014963793.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014963793.1

ORF Insert Sequence:

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3D ( XM_014963795.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014819281.1 Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa80738
Clone ID Related Accession (Same CDS sequence)	XM_014963794.1 , XM_014963796.1 , XM_014963798.1 , XM_014963793.1 , XM_014963795.1 , XM_014963792.1
Accession Version	XM_014963795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	semaphorin-3D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094506.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014819281.1
CDS	165..2450
Translation	MRASQVPHACSLLSLAMLFLPVTGTSKQNIPRLKLSYKDLLLSNSCIPFLGSTEGLDFRTLLLDEERGRLLIGAKDHIFLLNLVDLNKNVKKIYWPAAKEKVELCKLAGKDAHTECANFIRVLQPYNRTHVYVCGTGAFHPLCGYIELGTHKEETVFRLDTQNLESGRLKCPFDPQLPFASVMADEYLYAGTASDFLGKDTALTRSLGPSHDHHYIRTDISEHYWLTGAKFVGTFPIPDTYNPDDDKIYFFFREISQDSGTSDKTILSRVGRVCKNDMGGQRSLINKWTTFLKARLVCSIPGPEGADTHFDELQDIFLLSTRDERNPLVYGVFTTTSSVFKGSAVCVYSMADIRAVFNGPYAHKESVDHRWVQYEGRIPYPRPGTCPSKTYDPLIKSTRDFPDEVISFIKRHPLMYKSVYPVTGGPVFTRINVDYRLTQIVVDHVMAEDGQYDVIFLGTDIGTVLKVVSITKEKWTKEEVVLEELQIFKHPSFISTMEISQKQQQLYIGSRDGLVQLSLHRCHTYGKACADCCLARDPYCAWDGNSCSRYAPTSKRRARRQDVKYGDPVAQCWDVEDSISHETADEKVIFGIEFNSTFLECIPKSQQASIRWYIQRSGEEHREELKADERIIKTEHGLLIRSLQRKDAGAYFCKAQEHTFVHTIVKLNLNVIENGQMESTQKTEDEEGRVRDLLTESRLRYKDYIQLVSSPSFSLDEYCEQMWHREKRRQRNKGGAKWKHVQEMKKKRNRRHHEPARPPST

Target ORF information:

RefSeq Version	XM_014963795.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014963795.1

ORF Insert Sequence:

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3D ( XM_014963792.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014819278.1 Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X1, mRNA.

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CloneID	OCa80738
Clone ID Related Accession (Same CDS sequence)	XM_014963794.1 , XM_014963796.1 , XM_014963798.1 , XM_014963793.1 , XM_014963795.1 , XM_014963792.1
Accession Version	XM_014963792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	semaphorin-3D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094506.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014819278.1
CDS	503..2788
Translation	MRASQVPHACSLLSLAMLFLPVTGTSKQNIPRLKLSYKDLLLSNSCIPFLGSTEGLDFRTLLLDEERGRLLIGAKDHIFLLNLVDLNKNVKKIYWPAAKEKVELCKLAGKDAHTECANFIRVLQPYNRTHVYVCGTGAFHPLCGYIELGTHKEETVFRLDTQNLESGRLKCPFDPQLPFASVMADEYLYAGTASDFLGKDTALTRSLGPSHDHHYIRTDISEHYWLTGAKFVGTFPIPDTYNPDDDKIYFFFREISQDSGTSDKTILSRVGRVCKNDMGGQRSLINKWTTFLKARLVCSIPGPEGADTHFDELQDIFLLSTRDERNPLVYGVFTTTSSVFKGSAVCVYSMADIRAVFNGPYAHKESVDHRWVQYEGRIPYPRPGTCPSKTYDPLIKSTRDFPDEVISFIKRHPLMYKSVYPVTGGPVFTRINVDYRLTQIVVDHVMAEDGQYDVIFLGTDIGTVLKVVSITKEKWTKEEVVLEELQIFKHPSFISTMEISQKQQQLYIGSRDGLVQLSLHRCHTYGKACADCCLARDPYCAWDGNSCSRYAPTSKRRARRQDVKYGDPVAQCWDVEDSISHETADEKVIFGIEFNSTFLECIPKSQQASIRWYIQRSGEEHREELKADERIIKTEHGLLIRSLQRKDAGAYFCKAQEHTFVHTIVKLNLNVIENGQMESTQKTEDEEGRVRDLLTESRLRYKDYIQLVSSPSFSLDEYCEQMWHREKRRQRNKGGAKWKHVQEMKKKRNRRHHEPARPPST

Target ORF information:

RefSeq Version	XM_014963792.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014963792.1

ORF Insert Sequence:

ATGAGAGCTT CCCAAGTACC ACATGCCTGC AGCCTGTTAT CTTTAGCGAT GCTGTTCCTT 
CCAGTCACTG GAACGTCAAA GCAAAACATC CCAAGACTTA AGCTTTCCTA TAAAGACTTG 
CTGCTTTCAA ACAGCTGCAT CCCATTTCTG GGCTCAACGG AGGGGCTTGA TTTTCGAACC 
CTGTTGCTAG ATGAGGAGAG GGGCCGGCTG CTGATTGGGG CTAAAGACCA CATCTTCCTG 
CTCAACTTGG TTGATTTAAA CAAAAATGTA AAAAAGATTT ACTGGCCTGC AGCAAAAGAA 
AAGGTAGAAT TATGCAAATT AGCTGGGAAA GATGCCCATA CAGAATGTGC CAATTTTATC 
AGGGTTCTTC AGCCCTACAA CCGAACCCAT GTTTATGTCT GCGGCACAGG AGCATTTCAC 
CCTCTCTGTG GATACATTGA ACTTGGAACT CACAAAGAGG AAACAGTATT CCGGCTGGAT 
ACTCAGAACT TGGAGTCTGG CAGGTTGAAA TGCCCTTTTG ATCCCCAGCT GCCGTTTGCT 
TCAGTGATGG CAGATGAGTA TCTTTATGCT GGAACAGCTT CTGATTTCCT TGGCAAAGAC 
ACTGCTCTAA CACGTTCTCT GGGCCCTTCT CACGACCACC ATTACATCAG AACTGACATT 
TCGGAACATT ACTGGCTTAC TGGAGCAAAA TTCGTTGGAA CTTTTCCCAT CCCAGACACC 
TATAACCCAG ATGATGACAA AATCTACTTC TTTTTCCGAG AAATATCACA AGATAGTGGC 
ACATCTGACA AGACAATCCT TTCCCGAGTT GGAAGAGTTT GTAAGAATGA TATGGGAGGT 
CAGAGGAGCC TAATAAACAA GTGGACTACT TTTCTGAAAG CCAGACTTGT GTGTTCAATA 
CCTGGTCCTG AAGGAGCAGA CACACATTTT GATGAGCTGC AAGATATATT CTTACTGTCT 
ACAAGAGACG AGAGAAACCC ATTGGTATAT GGAGTCTTCA CTACAACAAG CTCTGTCTTC 
AAGGGCTCTG CAGTGTGTGT GTACAGCATG GCAGATATCA GAGCTGTTTT CAATGGCCCT 
TATGCTCACA AAGAGAGTGT GGATCATCGG TGGGTACAAT ATGAAGGTCG CATCCCGTAT 
CCCAGACCTG GTACATGTCC AAGCAAAACC TATGATCCAC TCATAAAGTC TACCAGAGAC 
TTTCCCGATG AAGTCATTAG CTTTATTAAA CGCCACCCAC TGATGTACAA ATCCGTTTAC 
CCAGTGACGG GAGGACCCGT ATTCACTCGA ATCAATGTGG ACTATCGTCT GACTCAGATT 
GTGGTGGATC ACGTCATGGC AGAGGATGGG CAATATGATG TTATTTTCCT AGGAACAGAC 
ATAGGTACAG TCTTGAAAGT TGTGAGTATC ACAAAGGAGA AGTGGACTAA GGAGGAGGTG 
GTCCTGGAAG AGCTGCAGAT ATTCAAGCAT CCTTCATTTA TCTCGACCAT GGAGATTTCT 
CAGAAGCAGC AACAATTGTA CATTGGTTCC AGGGATGGAT TGGTTCAGCT CTCTCTGCAC 
AGATGTCACA CGTATGGGAA GGCTTGTGCA GATTGTTGCC TTGCCAGAGA CCCATACTGT 
GCCTGGGATG GAAACTCCTG TTCCAGATAT GCCCCCACTT CTAAAAGGCG AGCCAGGAGG 
CAAGATGTCA AATATGGAGA CCCAGTTGCT CAGTGCTGGG ATGTGGAAGA CAGCATTAGT 
CATGAAACTG CTGATGAAAA GGTGATTTTT GGCATTGAAT TTAATTCAAC GTTTCTGGAA 
TGTATTCCTA AGTCCCAGCA AGCCTCTATT AGGTGGTATA TTCAGCGCTC TGGAGAAGAA 
CATCGAGAAG AGCTGAAGGC TGATGAAAGG ATCATCAAAA CAGAGCATGG GCTGCTGATC 
CGCAGCTTGC AAAGAAAGGA TGCAGGAGCT TACTTCTGCA AAGCCCAGGA GCACACCTTT 
GTCCATACCA TTGTGAAGCT GAATTTGAAT GTCATTGAGA ACGGGCAAAT GGAAAGCACT 
CAGAAAACTG AGGATGAGGA GGGCCGGGTG AGGGATTTGC TGACAGAGTC CCGGCTGAGG 
TACAAGGACT ACATTCAGCT CGTCAGCAGC CCCAGCTTTA GCCTGGATGA GTACTGTGAA 
CAGATGTGGC ACCGGGAGAA ACGGCGGCAG CGGAACAAAG GTGGTGCCAA ATGGAAGCAT 
GTGCAGGAGA TGAAAAAAAA GCGAAACCGA AGGCACCATG AGCCAGCCAG GCCACCTTCT 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SEMA3D cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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