TOP1MT cDNA ORF clone, Calidris pugnax(ruff)

The following TOP1MT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP1MT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa82056 XM_014965634.1
Latest version!
Calidris pugnax topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCa82057 XM_014965636.1
Latest version!
Calidris pugnax topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCa82057 XM_014965635.1
Latest version!
Calidris pugnax topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCa82057 XM_014965637.1
Latest version!
Calidris pugnax topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa82056
    Clone ID Related Accession (Same CDS sequence) XM_014965634.1
    Accession Version XM_014965634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product DNA topoisomerase I, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTACCGGC GTCTTGCCTC CTCGTTTCTC AGCCGTGGGG TCCCTCACCC CCAGCTCACC 
    CCCCTTTGCC TCCGGTCACG GTCGGGGGGC TGCAGCAGGT GGGAAGAGGA GAAGAAAGAA
    GATGGGGTGA AGTGGACGCA GCTGGAGCAC CGAGGACCCT ACTTTGCCCC CCTCTACGAA
    CCGCTGCCTG ATGATGTACA GTTCTACTAC GATGGCAAAC CCCTGAAGCT GAGCCTGGCC
    ACGGAGGAAA TTGCCACCTT CTATGCCAAA ATGCTCGATC ATGAATACAC GACCAAGGAG
    ATCTTCCAGA ACAACTTCTT CAATGACTGG AGGAAGGAGA TGACCTCGGA GGAGCAGGAG
    ATAATCAAGG ACCTGGACAA GTGTGACTTC AGGGAGATCC ACAAGTACTT TGTGGATAAA
    AGCGAGGCAC GGAAAGCACT CCCCAAAGAG GAGAAGCAGA AACTGAAGGA GGAGGCAGAT
    AAGATCCAGG AGGAGTATGG GTACTGCATC CTTGATGGGC ACCGGGAGAA GATAGGCAAC
    TTCAAAACAG AACCACCGGG GCTTTTCCGT GGCCGTGGTG ACCACCCCAA AATGGGCATG
    CTGAAGAAGA GGGTCATGCC AGAAGATGTT ATCATCAACT GCAGCAAGGA CTCCAAAATC
    CCAGAGCCTC CAGAAGGGCA CAAGTGGAAG GAGGTGCGCT TCGACAACAC AGTTACCTGG
    CTGGCCTCGT GGACAGAAAA CATCCAGAAC TGTTTGAAGT ACATCATGCT GAACCCCAGC
    TCCAAGCTGA AGGGGGAGAA AGACTGGCAG AAGTATGAGG TAGCCCGGCG CTTAAAGGGT
    GTCGTTCACA AAATCCGTGC TCAGTATCGA GCCGACTGGA AGTCCAAGGA GATGAAGAAG
    CGGCAAAGAG CAGTGGCTCT CTACTTCATT GATAAGCTGG CCCTGCGAGC TGGCAATGAG
    AAGGAGGAAG GGGAGACTGC TGACACAGTT GGCTGCTGCT CCCTGCGCGT GGAGCACATC
    AAACTGCACC CCAAGCTGGA TGGGCAGGAG CACGTGGTGG AGTTTGACTT CCTGGGGAAA
    GACTCCATCC GTTACTACAA CAAAGTGTCA GTGGAGAAGC TGGTGTTCAA GAACCTGCAG
    CTGTTCATGA AGAACAAGGA CCCAGGAGAT GACCTCTTTG ACAGGCTTAA TACATCCATT
    GTGAACAAAT ACCTCCAGAG CCTGATGGAT GGTTTGAGCG CCAAAGTGTT CAGGACTTAC
    AATGCCTCCA TCACCCTGCA GGAGCAGCTC AAGGCACTCA CTAATGCTGA GGACAATGTT
    GCGGGAAAGC TCCTCTCCTA CAACAGAGCT AATCGAGCCG TGGCCATCCT GTGCAACCAT
    CAGAGGTCTA CACCAAAGAC CTTTGAGAAG TCAATGCAAA ACCTCCAGGC CAAGATTGAT
    GCCAAGAAGA AACAGATTGA GGAAGCCCAG CAAGAGCTGA AAAAAGCTGA AGAGGAGTTT
    GAAAACACAA AAGATGCCAA AGCAGAAGCC AACGTGGAGA AGAAGAAGAA GCTGTTGAAG
    CGGCTGGAAG AGCAGCTGGC CAAGTTGAAC ATCCAGGCCA CAGATAAGGA GGAGAACAAG
    CAGATTGCTC TGGGCACCTC CAAGCTGAAT TACCTGGACC CCAGGATTAG CATAGCTTGG
    TGCAAGAAGT TCGGGGTGCC CATCGAGAAG ATCTACAACA AGACACAGAG GGAGAAGTTT
    GCCTGGGCGA TCGACATGGC CGACGAGGAC TTTGAATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014821120.1
    CDS162..1943
    Translation

    Target ORF information:

    RefSeq Version XM_014965634.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014965634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTACCGGC GTCTTGCCTC CTCGTTTCTC AGCCGTGGGG TCCCTCACCC CCAGCTCACC 
    CCCCTTTGCC TCCGGTCACG GTCGGGGGGC TGCAGCAGGT GGGAAGAGGA GAAGAAAGAA
    GATGGGGTGA AGTGGACGCA GCTGGAGCAC CGAGGACCCT ACTTTGCCCC CCTCTACGAA
    CCGCTGCCTG ATGATGTACA GTTCTACTAC GATGGCAAAC CCCTGAAGCT GAGCCTGGCC
    ACGGAGGAAA TTGCCACCTT CTATGCCAAA ATGCTCGATC ATGAATACAC GACCAAGGAG
    ATCTTCCAGA ACAACTTCTT CAATGACTGG AGGAAGGAGA TGACCTCGGA GGAGCAGGAG
    ATAATCAAGG ACCTGGACAA GTGTGACTTC AGGGAGATCC ACAAGTACTT TGTGGATAAA
    AGCGAGGCAC GGAAAGCACT CCCCAAAGAG GAGAAGCAGA AACTGAAGGA GGAGGCAGAT
    AAGATCCAGG AGGAGTATGG GTACTGCATC CTTGATGGGC ACCGGGAGAA GATAGGCAAC
    TTCAAAACAG AACCACCGGG GCTTTTCCGT GGCCGTGGTG ACCACCCCAA AATGGGCATG
    CTGAAGAAGA GGGTCATGCC AGAAGATGTT ATCATCAACT GCAGCAAGGA CTCCAAAATC
    CCAGAGCCTC CAGAAGGGCA CAAGTGGAAG GAGGTGCGCT TCGACAACAC AGTTACCTGG
    CTGGCCTCGT GGACAGAAAA CATCCAGAAC TGTTTGAAGT ACATCATGCT GAACCCCAGC
    TCCAAGCTGA AGGGGGAGAA AGACTGGCAG AAGTATGAGG TAGCCCGGCG CTTAAAGGGT
    GTCGTTCACA AAATCCGTGC TCAGTATCGA GCCGACTGGA AGTCCAAGGA GATGAAGAAG
    CGGCAAAGAG CAGTGGCTCT CTACTTCATT GATAAGCTGG CCCTGCGAGC TGGCAATGAG
    AAGGAGGAAG GGGAGACTGC TGACACAGTT GGCTGCTGCT CCCTGCGCGT GGAGCACATC
    AAACTGCACC CCAAGCTGGA TGGGCAGGAG CACGTGGTGG AGTTTGACTT CCTGGGGAAA
    GACTCCATCC GTTACTACAA CAAAGTGTCA GTGGAGAAGC TGGTGTTCAA GAACCTGCAG
    CTGTTCATGA AGAACAAGGA CCCAGGAGAT GACCTCTTTG ACAGGCTTAA TACATCCATT
    GTGAACAAAT ACCTCCAGAG CCTGATGGAT GGTTTGAGCG CCAAAGTGTT CAGGACTTAC
    AATGCCTCCA TCACCCTGCA GGAGCAGCTC AAGGCACTCA CTAATGCTGA GGACAATGTT
    GCGGGAAAGC TCCTCTCCTA CAACAGAGCT AATCGAGCCG TGGCCATCCT GTGCAACCAT
    CAGAGGTCTA CACCAAAGAC CTTTGAGAAG TCAATGCAAA ACCTCCAGGC CAAGATTGAT
    GCCAAGAAGA AACAGATTGA GGAAGCCCAG CAAGAGCTGA AAAAAGCTGA AGAGGAGTTT
    GAAAACACAA AAGATGCCAA AGCAGAAGCC AACGTGGAGA AGAAGAAGAA GCTGTTGAAG
    CGGCTGGAAG AGCAGCTGGC CAAGTTGAAC ATCCAGGCCA CAGATAAGGA GGAGAACAAG
    CAGATTGCTC TGGGCACCTC CAAGCTGAAT TACCTGGACC CCAGGATTAG CATAGCTTGG
    TGCAAGAAGT TCGGGGTGCC CATCGAGAAG ATCTACAACA AGACACAGAG GGAGAAGTTT
    GCCTGGGCGA TCGACATGGC CGACGAGGAC TTTGAATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa82057
    Clone ID Related Accession (Same CDS sequence) XM_014965636.1 , XM_014965635.1 , XM_014965637.1
    Accession Version XM_014965636.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product DNA topoisomerase I, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTCGATC ATGAATACAC GACCAAGGAG ATCTTCCAGA ACAACTTCTT CAATGACTGG 
    AGGAAGGAGA TGACCTCGGA GGAGCAGGAG ATAATCAAGG ACCTGGACAA GTGTGACTTC
    AGGGAGATCC ACAAGTACTT TGTGGATAAA AGCGAGGCAC GGAAAGCACT CCCCAAAGAG
    GAGAAGCAGA AACTGAAGGA GGAGGCAGAT AAGATCCAGG AGGAGTATGG GTACTGCATC
    CTTGATGGGC ACCGGGAGAA GATAGGCAAC TTCAAAACAG AACCACCGGG GCTTTTCCGT
    GGCCGTGGTG ACCACCCCAA AATGGGCATG CTGAAGAAGA GGGTCATGCC AGAAGATGTT
    ATCATCAACT GCAGCAAGGA CTCCAAAATC CCAGAGCCTC CAGAAGGGCA CAAGTGGAAG
    GAGGTGCGCT TCGACAACAC AGTTACCTGG CTGGCCTCGT GGACAGAAAA CATCCAGAAC
    TGTTTGAAGT ACATCATGCT GAACCCCAGC TCCAAGCTGA AGGGGGAGAA AGACTGGCAG
    AAGTATGAGG TAGCCCGGCG CTTAAAGGGT GTCGTTCACA AAATCCGTGC TCAGTATCGA
    GCCGACTGGA AGTCCAAGGA GATGAAGAAG CGGCAAAGAG CAGTGGCTCT CTACTTCATT
    GATAAGCTGG CCCTGCGAGC TGGCAATGAG AAGGAGGAAG GGGAGACTGC TGACACAGTT
    GGCTGCTGCT CCCTGCGCGT GGAGCACATC AAACTGCACC CCAAGCTGGA TGGGCAGGAG
    CACGTGGTGG AGTTTGACTT CCTGGGGAAA GACTCCATCC GTTACTACAA CAAAGTGTCA
    GTGGAGAAGC TGGTGTTCAA GAACCTGCAG CTGTTCATGA AGAACAAGGA CCCAGGAGAT
    GACCTCTTTG ACAGGCTTAA TACATCCATT GTGAACAAAT ACCTCCAGAG CCTGATGGAT
    GGTTTGAGCG CCAAAGTGTT CAGGACTTAC AATGCCTCCA TCACCCTGCA GGAGCAGCTC
    AAGGCACTCA CTAATGCTGA GGACAATGTT GCGGGAAAGC TCCTCTCCTA CAACAGAGCT
    AATCGAGCCG TGGCCATCCT GTGCAACCAT CAGAGGTCTA CACCAAAGAC CTTTGAGAAG
    TCAATGCAAA ACCTCCAGGC CAAGATTGAT GCCAAGAAGA AACAGATTGA GGAAGCCCAG
    CAAGAGCTGA AAAAAGCTGA AGAGGAGTTT GAAAACACAA AAGATGCCAA AGCAGAAGCC
    AACGTGGAGA AGAAGAAGAA GCTGTTGAAG CGGCTGGAAG AGCAGCTGGC CAAGTTGAAC
    ATCCAGGCCA CAGATAAGGA GGAGAACAAG CAGATTGCTC TGGGCACCTC CAAGCTGAAT
    TACCTGGACC CCAGGATTAG CATAGCTTGG TGCAAGAAGT TCGGGGTGCC CATCGAGAAG
    ATCTACAACA AGACACAGAG GGAGAAGTTT GCCTGGGCGA TCGACATGGC CGACGAGGAC
    TTTGAATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014821122.1
    CDS351..1862
    Translation

    Target ORF information:

    RefSeq Version XM_014965636.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014965636.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTCGATC ATGAATACAC GACCAAGGAG ATCTTCCAGA ACAACTTCTT CAATGACTGG 
    AGGAAGGAGA TGACCTCGGA GGAGCAGGAG ATAATCAAGG ACCTGGACAA GTGTGACTTC
    AGGGAGATCC ACAAGTACTT TGTGGATAAA AGCGAGGCAC GGAAAGCACT CCCCAAAGAG
    GAGAAGCAGA AACTGAAGGA GGAGGCAGAT AAGATCCAGG AGGAGTATGG GTACTGCATC
    CTTGATGGGC ACCGGGAGAA GATAGGCAAC TTCAAAACAG AACCACCGGG GCTTTTCCGT
    GGCCGTGGTG ACCACCCCAA AATGGGCATG CTGAAGAAGA GGGTCATGCC AGAAGATGTT
    ATCATCAACT GCAGCAAGGA CTCCAAAATC CCAGAGCCTC CAGAAGGGCA CAAGTGGAAG
    GAGGTGCGCT TCGACAACAC AGTTACCTGG CTGGCCTCGT GGACAGAAAA CATCCAGAAC
    TGTTTGAAGT ACATCATGCT GAACCCCAGC TCCAAGCTGA AGGGGGAGAA AGACTGGCAG
    AAGTATGAGG TAGCCCGGCG CTTAAAGGGT GTCGTTCACA AAATCCGTGC TCAGTATCGA
    GCCGACTGGA AGTCCAAGGA GATGAAGAAG CGGCAAAGAG CAGTGGCTCT CTACTTCATT
    GATAAGCTGG CCCTGCGAGC TGGCAATGAG AAGGAGGAAG GGGAGACTGC TGACACAGTT
    GGCTGCTGCT CCCTGCGCGT GGAGCACATC AAACTGCACC CCAAGCTGGA TGGGCAGGAG
    CACGTGGTGG AGTTTGACTT CCTGGGGAAA GACTCCATCC GTTACTACAA CAAAGTGTCA
    GTGGAGAAGC TGGTGTTCAA GAACCTGCAG CTGTTCATGA AGAACAAGGA CCCAGGAGAT
    GACCTCTTTG ACAGGCTTAA TACATCCATT GTGAACAAAT ACCTCCAGAG CCTGATGGAT
    GGTTTGAGCG CCAAAGTGTT CAGGACTTAC AATGCCTCCA TCACCCTGCA GGAGCAGCTC
    AAGGCACTCA CTAATGCTGA GGACAATGTT GCGGGAAAGC TCCTCTCCTA CAACAGAGCT
    AATCGAGCCG TGGCCATCCT GTGCAACCAT CAGAGGTCTA CACCAAAGAC CTTTGAGAAG
    TCAATGCAAA ACCTCCAGGC CAAGATTGAT GCCAAGAAGA AACAGATTGA GGAAGCCCAG
    CAAGAGCTGA AAAAAGCTGA AGAGGAGTTT GAAAACACAA AAGATGCCAA AGCAGAAGCC
    AACGTGGAGA AGAAGAAGAA GCTGTTGAAG CGGCTGGAAG AGCAGCTGGC CAAGTTGAAC
    ATCCAGGCCA CAGATAAGGA GGAGAACAAG CAGATTGCTC TGGGCACCTC CAAGCTGAAT
    TACCTGGACC CCAGGATTAG CATAGCTTGG TGCAAGAAGT TCGGGGTGCC CATCGAGAAG
    ATCTACAACA AGACACAGAG GGAGAAGTTT GCCTGGGCGA TCGACATGGC CGACGAGGAC
    TTTGAATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa82057
    Clone ID Related Accession (Same CDS sequence) XM_014965636.1 , XM_014965635.1 , XM_014965637.1
    Accession Version XM_014965635.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product DNA topoisomerase I, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTCGATC ATGAATACAC GACCAAGGAG ATCTTCCAGA ACAACTTCTT CAATGACTGG 
    AGGAAGGAGA TGACCTCGGA GGAGCAGGAG ATAATCAAGG ACCTGGACAA GTGTGACTTC
    AGGGAGATCC ACAAGTACTT TGTGGATAAA AGCGAGGCAC GGAAAGCACT CCCCAAAGAG
    GAGAAGCAGA AACTGAAGGA GGAGGCAGAT AAGATCCAGG AGGAGTATGG GTACTGCATC
    CTTGATGGGC ACCGGGAGAA GATAGGCAAC TTCAAAACAG AACCACCGGG GCTTTTCCGT
    GGCCGTGGTG ACCACCCCAA AATGGGCATG CTGAAGAAGA GGGTCATGCC AGAAGATGTT
    ATCATCAACT GCAGCAAGGA CTCCAAAATC CCAGAGCCTC CAGAAGGGCA CAAGTGGAAG
    GAGGTGCGCT TCGACAACAC AGTTACCTGG CTGGCCTCGT GGACAGAAAA CATCCAGAAC
    TGTTTGAAGT ACATCATGCT GAACCCCAGC TCCAAGCTGA AGGGGGAGAA AGACTGGCAG
    AAGTATGAGG TAGCCCGGCG CTTAAAGGGT GTCGTTCACA AAATCCGTGC TCAGTATCGA
    GCCGACTGGA AGTCCAAGGA GATGAAGAAG CGGCAAAGAG CAGTGGCTCT CTACTTCATT
    GATAAGCTGG CCCTGCGAGC TGGCAATGAG AAGGAGGAAG GGGAGACTGC TGACACAGTT
    GGCTGCTGCT CCCTGCGCGT GGAGCACATC AAACTGCACC CCAAGCTGGA TGGGCAGGAG
    CACGTGGTGG AGTTTGACTT CCTGGGGAAA GACTCCATCC GTTACTACAA CAAAGTGTCA
    GTGGAGAAGC TGGTGTTCAA GAACCTGCAG CTGTTCATGA AGAACAAGGA CCCAGGAGAT
    GACCTCTTTG ACAGGCTTAA TACATCCATT GTGAACAAAT ACCTCCAGAG CCTGATGGAT
    GGTTTGAGCG CCAAAGTGTT CAGGACTTAC AATGCCTCCA TCACCCTGCA GGAGCAGCTC
    AAGGCACTCA CTAATGCTGA GGACAATGTT GCGGGAAAGC TCCTCTCCTA CAACAGAGCT
    AATCGAGCCG TGGCCATCCT GTGCAACCAT CAGAGGTCTA CACCAAAGAC CTTTGAGAAG
    TCAATGCAAA ACCTCCAGGC CAAGATTGAT GCCAAGAAGA AACAGATTGA GGAAGCCCAG
    CAAGAGCTGA AAAAAGCTGA AGAGGAGTTT GAAAACACAA AAGATGCCAA AGCAGAAGCC
    AACGTGGAGA AGAAGAAGAA GCTGTTGAAG CGGCTGGAAG AGCAGCTGGC CAAGTTGAAC
    ATCCAGGCCA CAGATAAGGA GGAGAACAAG CAGATTGCTC TGGGCACCTC CAAGCTGAAT
    TACCTGGACC CCAGGATTAG CATAGCTTGG TGCAAGAAGT TCGGGGTGCC CATCGAGAAG
    ATCTACAACA AGACACAGAG GGAGAAGTTT GCCTGGGCGA TCGACATGGC CGACGAGGAC
    TTTGAATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014821121.1
    CDS293..1804
    Translation

    Target ORF information:

    RefSeq Version XM_014965635.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014965635.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTCGATC ATGAATACAC GACCAAGGAG ATCTTCCAGA ACAACTTCTT CAATGACTGG 
    AGGAAGGAGA TGACCTCGGA GGAGCAGGAG ATAATCAAGG ACCTGGACAA GTGTGACTTC
    AGGGAGATCC ACAAGTACTT TGTGGATAAA AGCGAGGCAC GGAAAGCACT CCCCAAAGAG
    GAGAAGCAGA AACTGAAGGA GGAGGCAGAT AAGATCCAGG AGGAGTATGG GTACTGCATC
    CTTGATGGGC ACCGGGAGAA GATAGGCAAC TTCAAAACAG AACCACCGGG GCTTTTCCGT
    GGCCGTGGTG ACCACCCCAA AATGGGCATG CTGAAGAAGA GGGTCATGCC AGAAGATGTT
    ATCATCAACT GCAGCAAGGA CTCCAAAATC CCAGAGCCTC CAGAAGGGCA CAAGTGGAAG
    GAGGTGCGCT TCGACAACAC AGTTACCTGG CTGGCCTCGT GGACAGAAAA CATCCAGAAC
    TGTTTGAAGT ACATCATGCT GAACCCCAGC TCCAAGCTGA AGGGGGAGAA AGACTGGCAG
    AAGTATGAGG TAGCCCGGCG CTTAAAGGGT GTCGTTCACA AAATCCGTGC TCAGTATCGA
    GCCGACTGGA AGTCCAAGGA GATGAAGAAG CGGCAAAGAG CAGTGGCTCT CTACTTCATT
    GATAAGCTGG CCCTGCGAGC TGGCAATGAG AAGGAGGAAG GGGAGACTGC TGACACAGTT
    GGCTGCTGCT CCCTGCGCGT GGAGCACATC AAACTGCACC CCAAGCTGGA TGGGCAGGAG
    CACGTGGTGG AGTTTGACTT CCTGGGGAAA GACTCCATCC GTTACTACAA CAAAGTGTCA
    GTGGAGAAGC TGGTGTTCAA GAACCTGCAG CTGTTCATGA AGAACAAGGA CCCAGGAGAT
    GACCTCTTTG ACAGGCTTAA TACATCCATT GTGAACAAAT ACCTCCAGAG CCTGATGGAT
    GGTTTGAGCG CCAAAGTGTT CAGGACTTAC AATGCCTCCA TCACCCTGCA GGAGCAGCTC
    AAGGCACTCA CTAATGCTGA GGACAATGTT GCGGGAAAGC TCCTCTCCTA CAACAGAGCT
    AATCGAGCCG TGGCCATCCT GTGCAACCAT CAGAGGTCTA CACCAAAGAC CTTTGAGAAG
    TCAATGCAAA ACCTCCAGGC CAAGATTGAT GCCAAGAAGA AACAGATTGA GGAAGCCCAG
    CAAGAGCTGA AAAAAGCTGA AGAGGAGTTT GAAAACACAA AAGATGCCAA AGCAGAAGCC
    AACGTGGAGA AGAAGAAGAA GCTGTTGAAG CGGCTGGAAG AGCAGCTGGC CAAGTTGAAC
    ATCCAGGCCA CAGATAAGGA GGAGAACAAG CAGATTGCTC TGGGCACCTC CAAGCTGAAT
    TACCTGGACC CCAGGATTAG CATAGCTTGG TGCAAGAAGT TCGGGGTGCC CATCGAGAAG
    ATCTACAACA AGACACAGAG GGAGAAGTTT GCCTGGGCGA TCGACATGGC CGACGAGGAC
    TTTGAATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa82057
    Clone ID Related Accession (Same CDS sequence) XM_014965636.1 , XM_014965635.1 , XM_014965637.1
    Accession Version XM_014965637.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product DNA topoisomerase I, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTCGATC ATGAATACAC GACCAAGGAG ATCTTCCAGA ACAACTTCTT CAATGACTGG 
    AGGAAGGAGA TGACCTCGGA GGAGCAGGAG ATAATCAAGG ACCTGGACAA GTGTGACTTC
    AGGGAGATCC ACAAGTACTT TGTGGATAAA AGCGAGGCAC GGAAAGCACT CCCCAAAGAG
    GAGAAGCAGA AACTGAAGGA GGAGGCAGAT AAGATCCAGG AGGAGTATGG GTACTGCATC
    CTTGATGGGC ACCGGGAGAA GATAGGCAAC TTCAAAACAG AACCACCGGG GCTTTTCCGT
    GGCCGTGGTG ACCACCCCAA AATGGGCATG CTGAAGAAGA GGGTCATGCC AGAAGATGTT
    ATCATCAACT GCAGCAAGGA CTCCAAAATC CCAGAGCCTC CAGAAGGGCA CAAGTGGAAG
    GAGGTGCGCT TCGACAACAC AGTTACCTGG CTGGCCTCGT GGACAGAAAA CATCCAGAAC
    TGTTTGAAGT ACATCATGCT GAACCCCAGC TCCAAGCTGA AGGGGGAGAA AGACTGGCAG
    AAGTATGAGG TAGCCCGGCG CTTAAAGGGT GTCGTTCACA AAATCCGTGC TCAGTATCGA
    GCCGACTGGA AGTCCAAGGA GATGAAGAAG CGGCAAAGAG CAGTGGCTCT CTACTTCATT
    GATAAGCTGG CCCTGCGAGC TGGCAATGAG AAGGAGGAAG GGGAGACTGC TGACACAGTT
    GGCTGCTGCT CCCTGCGCGT GGAGCACATC AAACTGCACC CCAAGCTGGA TGGGCAGGAG
    CACGTGGTGG AGTTTGACTT CCTGGGGAAA GACTCCATCC GTTACTACAA CAAAGTGTCA
    GTGGAGAAGC TGGTGTTCAA GAACCTGCAG CTGTTCATGA AGAACAAGGA CCCAGGAGAT
    GACCTCTTTG ACAGGCTTAA TACATCCATT GTGAACAAAT ACCTCCAGAG CCTGATGGAT
    GGTTTGAGCG CCAAAGTGTT CAGGACTTAC AATGCCTCCA TCACCCTGCA GGAGCAGCTC
    AAGGCACTCA CTAATGCTGA GGACAATGTT GCGGGAAAGC TCCTCTCCTA CAACAGAGCT
    AATCGAGCCG TGGCCATCCT GTGCAACCAT CAGAGGTCTA CACCAAAGAC CTTTGAGAAG
    TCAATGCAAA ACCTCCAGGC CAAGATTGAT GCCAAGAAGA AACAGATTGA GGAAGCCCAG
    CAAGAGCTGA AAAAAGCTGA AGAGGAGTTT GAAAACACAA AAGATGCCAA AGCAGAAGCC
    AACGTGGAGA AGAAGAAGAA GCTGTTGAAG CGGCTGGAAG AGCAGCTGGC CAAGTTGAAC
    ATCCAGGCCA CAGATAAGGA GGAGAACAAG CAGATTGCTC TGGGCACCTC CAAGCTGAAT
    TACCTGGACC CCAGGATTAG CATAGCTTGG TGCAAGAAGT TCGGGGTGCC CATCGAGAAG
    ATCTACAACA AGACACAGAG GGAGAAGTTT GCCTGGGCGA TCGACATGGC CGACGAGGAC
    TTTGAATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014821123.1
    CDS192..1703
    Translation

    Target ORF information:

    RefSeq Version XM_014965637.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014965637.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTCGATC ATGAATACAC GACCAAGGAG ATCTTCCAGA ACAACTTCTT CAATGACTGG 
    AGGAAGGAGA TGACCTCGGA GGAGCAGGAG ATAATCAAGG ACCTGGACAA GTGTGACTTC
    AGGGAGATCC ACAAGTACTT TGTGGATAAA AGCGAGGCAC GGAAAGCACT CCCCAAAGAG
    GAGAAGCAGA AACTGAAGGA GGAGGCAGAT AAGATCCAGG AGGAGTATGG GTACTGCATC
    CTTGATGGGC ACCGGGAGAA GATAGGCAAC TTCAAAACAG AACCACCGGG GCTTTTCCGT
    GGCCGTGGTG ACCACCCCAA AATGGGCATG CTGAAGAAGA GGGTCATGCC AGAAGATGTT
    ATCATCAACT GCAGCAAGGA CTCCAAAATC CCAGAGCCTC CAGAAGGGCA CAAGTGGAAG
    GAGGTGCGCT TCGACAACAC AGTTACCTGG CTGGCCTCGT GGACAGAAAA CATCCAGAAC
    TGTTTGAAGT ACATCATGCT GAACCCCAGC TCCAAGCTGA AGGGGGAGAA AGACTGGCAG
    AAGTATGAGG TAGCCCGGCG CTTAAAGGGT GTCGTTCACA AAATCCGTGC TCAGTATCGA
    GCCGACTGGA AGTCCAAGGA GATGAAGAAG CGGCAAAGAG CAGTGGCTCT CTACTTCATT
    GATAAGCTGG CCCTGCGAGC TGGCAATGAG AAGGAGGAAG GGGAGACTGC TGACACAGTT
    GGCTGCTGCT CCCTGCGCGT GGAGCACATC AAACTGCACC CCAAGCTGGA TGGGCAGGAG
    CACGTGGTGG AGTTTGACTT CCTGGGGAAA GACTCCATCC GTTACTACAA CAAAGTGTCA
    GTGGAGAAGC TGGTGTTCAA GAACCTGCAG CTGTTCATGA AGAACAAGGA CCCAGGAGAT
    GACCTCTTTG ACAGGCTTAA TACATCCATT GTGAACAAAT ACCTCCAGAG CCTGATGGAT
    GGTTTGAGCG CCAAAGTGTT CAGGACTTAC AATGCCTCCA TCACCCTGCA GGAGCAGCTC
    AAGGCACTCA CTAATGCTGA GGACAATGTT GCGGGAAAGC TCCTCTCCTA CAACAGAGCT
    AATCGAGCCG TGGCCATCCT GTGCAACCAT CAGAGGTCTA CACCAAAGAC CTTTGAGAAG
    TCAATGCAAA ACCTCCAGGC CAAGATTGAT GCCAAGAAGA AACAGATTGA GGAAGCCCAG
    CAAGAGCTGA AAAAAGCTGA AGAGGAGTTT GAAAACACAA AAGATGCCAA AGCAGAAGCC
    AACGTGGAGA AGAAGAAGAA GCTGTTGAAG CGGCTGGAAG AGCAGCTGGC CAAGTTGAAC
    ATCCAGGCCA CAGATAAGGA GGAGAACAAG CAGATTGCTC TGGGCACCTC CAAGCTGAAT
    TACCTGGACC CCAGGATTAG CATAGCTTGG TGCAAGAAGT TCGGGGTGCC CATCGAGAAG
    ATCTACAACA AGACACAGAG GGAGAAGTTT GCCTGGGCGA TCGACATGGC CGACGAGGAC
    TTTGAATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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