AFDN gene cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset)

mRNA	Protein	Name
XM_017971545.1	XP_017827034.1	afadin isoform X1
XM_002747188.3	XP_002747234.3	afadin isoform X6
XM_017971552.1	XP_017827041.1	afadin isoform X9
XM_017971555.1	XP_017827044.1	afadin isoform X12
XM_017971546.1	XP_017827035.1	afadin isoform X2
XM_017971548.1	XP_017827037.1	afadin isoform X4
XM_017971553.1	XP_017827042.1	afadin isoform X10
XM_017971556.1	XP_017827045.1	afadin isoform X13
XM_017971549.1	XP_017827038.1	afadin isoform X5
XM_017971554.1	XP_017827043.1	afadin isoform X11
XM_017971557.1	XP_017827046.1	afadin isoform X14
XM_017971547.1	XP_017827036.1	afadin isoform X3
XM_017971550.1	XP_017827039.1	afadin isoform X7
XM_017971551.1	XP_017827040.1	afadin isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCb30577	XM_002747188.3 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53670	XM_017971545.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53671	XM_017971546.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53672	XM_017971547.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53673	XM_017971548.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53674	XM_017971549.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53675	XM_017971550.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53676	XM_017971551.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53677	XM_017971552.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53678	XM_017971553.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53679	XM_017971554.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCb53680	XM_017971555.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCb53681	XM_017971556.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCb53682	XM_017971557.1 Latest version!	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

AFDN ( XM_002747188.3 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_002747234.3 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCb30577
Clone ID Related Accession (Same CDS sequence)	XM_002747188.3
Accession Version	XM_002747188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 2, 2014 this sequence version replaced XM_002747188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGAATCACAT TGGATGCCCA AGATGTTCTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGACGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATTCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACTGACCC AGATTCAGGG CTGTGCTCAC ATTACTGGGG CGCGATCATC 
CGTCAGCAGC TGGGCCATAT TGAAGCATGG GCTGAGAAGC AGGGGCTGGA GCTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT TCAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGGCTGTGGC TGAAAACACT GCTGATGAGC TGGCCCGCAG CGATGGAAGG 
GAGGTGCAGT TGGAGGAGGA TCCTGACCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCCCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCGGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTCAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCC 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGGCTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTCC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAGC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGATAGAC TGATGAAAAA CAGAGCTGAT CACCGCTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AGGTGCATAT GCCTCTGGAA CAGCAGCGAA GATAACATCT 
GTCTCTACTG GGAACCTCTG CACCGAGGAG CAGAGTCCTC CGCCGAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTCACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTACG AAGAAAAGCC ACATACGCAC 
ACAGACAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAACTTACCA GCTTGAGCGG CATCGAATAG AGGTGGCTCT AGAACGCAAG 
TCCGATAGTG ATATGTGGGT AAATCAGAGC TCCTCACTGG ACTCCAGTAC ATCTAGCCAA 
GAGCACCTGA ACCATTCCTC TAAGCCGGTC ACCCCTGCTT CCACGCTGAC CAAAAGCGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCC 
CTCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCCG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATCT GCCTCTCCCA CCACCTCCTT CTGCCAACCA GATAGGACTG 
CCGTCTGCGC AGGTGGCTGC TGCAGAACGG AAAAAGAGGG AAGAGCATCA GCGCTGGTAT 
GAGAAGGAAA AGGCCCGCCT GGAAGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCCTA AACCCTGCTC CGTTTTCTCC CCTCACCACA 
CAACAGATGA AGCCCGAAAA GCCTACCACC CTCCAGAGGC CCCAAGAAAC CGTCATCCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACC ATCGAGCGCA GGGACCTGCA GTACATCACA 
GTTAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CTGACCCGTG GAAGCGCGAC 
GCTAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCAGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAATT CCAGAAGCGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAC 
GACGAGGAGG AGGAGGATGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGACAGCAGC AGTTAGAAGA GATGCGTAAA 
CGCGAAGCAG AAGACCGAGC AAGACAAGAG GAAGAGCGCC ATCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA TGACGAGGCG GCACGCAGGT TGTTGGAGCC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCAGGAC TACGAGCCCC AGTCCCCGTC CCCCACGCCC 
AGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCTGACTCGC TGTTCACTGC CAAGTTTGTT GCGTACAATG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GACCGAACTC TTACCCAGGA TCTACGGGGG CAGCTGTTGG AACCCATGAG 
GCGTATCGGG ATGCAAGAGA GAAGCGCTCT AAAAGCCAAG ATGCAGATGT CCCTGGAAGT 
TCTGGGGCCC CTGAAAACTT GACATTCAAA GAACGCCAGC GTCTTTTTTC ACAAGGTCAA 
GATGTATCCA ATAAAGTGAA AGCTTCTCGT AAATTAACAG AACTGGAGAA TGAACTGAAC 
ACAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002747234.3
CDS	112..5580
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPQDYEPQSPSPTPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEDCSLAGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_002747188.3
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002747188.3

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGAATCACAT TGGATGCCCA AGATGTTCTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGACGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATTCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACTGACCC AGATTCAGGG CTGTGCTCAC ATTACTGGGG CGCGATCATC 
CGTCAGCAGC TGGGCCATAT TGAAGCATGG GCTGAGAAGC AGGGGCTGGA GCTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT TCAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGGCTGTGGC TGAAAACACT GCTGATGAGC TGGCCCGCAG CGATGGAAGG 
GAGGTGCAGT TGGAGGAGGA TCCTGACCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCCCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCGGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTCAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCC 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGGCTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTCC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAGC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGATAGAC TGATGAAAAA CAGAGCTGAT CACCGCTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AGGTGCATAT GCCTCTGGAA CAGCAGCGAA GATAACATCT 
GTCTCTACTG GGAACCTCTG CACCGAGGAG CAGAGTCCTC CGCCGAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTCACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTACG AAGAAAAGCC ACATACGCAC 
ACAGACAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAACTTACCA GCTTGAGCGG CATCGAATAG AGGTGGCTCT AGAACGCAAG 
TCCGATAGTG ATATGTGGGT AAATCAGAGC TCCTCACTGG ACTCCAGTAC ATCTAGCCAA 
GAGCACCTGA ACCATTCCTC TAAGCCGGTC ACCCCTGCTT CCACGCTGAC CAAAAGCGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCGT CTCCCAGCCC 
CTCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCCG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATCT GCCTCTCCCA CCACCTCCTT CTGCCAACCA GATAGGACTG 
CCGTCTGCGC AGGTGGCTGC TGCAGAACGG AAAAAGAGGG AAGAGCATCA GCGCTGGTAT 
GAGAAGGAAA AGGCCCGCCT GGAAGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCCTA AACCCTGCTC CGTTTTCTCC CCTCACCACA 
CAACAGATGA AGCCCGAAAA GCCTACCACC CTCCAGAGGC CCCAAGAAAC CGTCATCCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACC ATCGAGCGCA GGGACCTGCA GTACATCACA 
GTTAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CTGACCCGTG GAAGCGCGAC 
GCTAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCAGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAATT CCAGAAGCGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAC 
GACGAGGAGG AGGAGGATGA TGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGACAGCAGC AGTTAGAAGA GATGCGTAAA 
CGCGAAGCAG AAGACCGAGC AAGACAAGAG GAAGAGCGCC ATCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA TGACGAGGCG GCACGCAGGT TGTTGGAGCC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCAGGAC TACGAGCCCC AGTCCCCGTC CCCCACGCCC 
AGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCTGACTCGC TGTTCACTGC CAAGTTTGTT GCGTACAATG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GACCGAACTC TTACCCAGGA TCTACGGGGG CAGCTGTTGG AACCCATGAG 
GCGTATCGGG ATGCAAGAGA GAAGCGCTCT AAAAGCCAAG ATGCAGATGT CCCTGGAAGT 
TCTGGGGCCC CTGAAAACTT GACATTCAAA GAACGCCAGC GTCTTTTTTC ACAAGGTCAA 
GATGTATCCA ATAAAGTGAA AGCTTCTCGT AAATTAACAG AACTGGAGAA TGAACTGAAC 
ACAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971545.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827034.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

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CloneID	OCb53670
Clone ID Related Accession (Same CDS sequence)	XM_017971545.1
Accession Version	XM_017971545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTCTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGACGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATTCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACTGACCCAG ATTCAGGGCT GTGCTCACAT 
TACTGGGGCG CGATCATCCG TCAGCAGCTG GGCCATATTG AAGCATGGGC TGAGAAGCAG 
GGGCTGGAGC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTTC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG GCTGTGGCTG AAAACACTGC TGATGAGCTG 
GCCCGCAGCG ATGGAAGGGA GGTGCAGTTG GAGGAGGATC CTGACCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCCCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCGGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT CAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCCGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGGCTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTCCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAGCTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGATAGACTG ATGAAAAACA GAGCTGATCA CCGCTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAG GTGCATATGC CTCTGGAACA 
GCAGCGAAGA TAACATCTGT CTCTACTGGG AACCTCTGCA CCGAGGAGCA GAGTCCTCCG 
CCGAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTCACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTACGAA 
GAAAAGCCAC ATACGCACAC AGACAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA ACTTACCAGC TTGAGCGGCA TCGAATAGAG 
GTGGCTCTAG AACGCAAGTC CGATAGTGAT ATGTGGGTAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACAT CTAGCCAAGA GCACCTGAAC CATTCCTCTA AGCCGGTCAC CCCTGCTTCC 
ACGCTGACCA AAAGCGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCGTCT CCCAGCCCCT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCCGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATCTGC CTCTCCCACC ACCTCCTTCT 
GCCAACCAGA TAGGACTGCC GTCTGCGCAG GTGGCTGCTG CAGAACGGAA AAAGAGGGAA 
GAGCATCAGC GCTGGTATGA GAAGGAAAAG GCCCGCCTGG AAGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCCTAAA CCCTGCTCCG 
TTTTCTCCCC TCACCACACA ACAGATGAAG CCCGAAAAGC CTACCACCCT CCAGAGGCCC 
CAAGAAACCG TCATCCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACAGT TAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCT 
GACCCGTGGA AGCGCGACGC TAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CAGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAATTCC AGAAGCGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGACGA CGAGGAGGAG GAGGATGATG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGACAG 
CAGCAGTTAG AAGAGATGCG TAAACGCGAA GCAGAAGACC GAGCAAGACA AGAGGAAGAG 
CGCCATCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA 
GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCATGACGA GGCGGCACGC 
AGGTTGTTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCA GGACTACGAG 
CCCCAGTCCC CGTCCCCCAC GCCCAGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC 
TACCTCAAAA CACAGGTCCT CTCCCCTGAC TCGCTGTTCA CTGCCAAGTT TGTTGCGTAC 
AATGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCGA ACTCTTACCC AGGATCTACG 
GGGGCAGCTG TTGGAACCCA TGAGGCGTAT CGGGATGCAA GAGAGAAGCG CTCTAAAAGC 
CAAGATGCAG ATGTCCCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAAGAACGC 
CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA 
ACAGAACTGG AGAATGAACT GAACACAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827034.1
CDS	112..5604
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPQDYEPQSPSPTPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEDCSLAGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971545.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971545.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTCTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGACGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATTCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACTGACCCAG ATTCAGGGCT GTGCTCACAT 
TACTGGGGCG CGATCATCCG TCAGCAGCTG GGCCATATTG AAGCATGGGC TGAGAAGCAG 
GGGCTGGAGC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTTC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG GCTGTGGCTG AAAACACTGC TGATGAGCTG 
GCCCGCAGCG ATGGAAGGGA GGTGCAGTTG GAGGAGGATC CTGACCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCCCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCGGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT CAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCCGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGGCTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTCCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAGCTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGATAGACTG ATGAAAAACA GAGCTGATCA CCGCTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAG GTGCATATGC CTCTGGAACA 
GCAGCGAAGA TAACATCTGT CTCTACTGGG AACCTCTGCA CCGAGGAGCA GAGTCCTCCG 
CCGAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTCACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTACGAA 
GAAAAGCCAC ATACGCACAC AGACAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA ACTTACCAGC TTGAGCGGCA TCGAATAGAG 
GTGGCTCTAG AACGCAAGTC CGATAGTGAT ATGTGGGTAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACAT CTAGCCAAGA GCACCTGAAC CATTCCTCTA AGCCGGTCAC CCCTGCTTCC 
ACGCTGACCA AAAGCGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCGTCT CCCAGCCCCT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCCGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATCTGC CTCTCCCACC ACCTCCTTCT 
GCCAACCAGA TAGGACTGCC GTCTGCGCAG GTGGCTGCTG CAGAACGGAA AAAGAGGGAA 
GAGCATCAGC GCTGGTATGA GAAGGAAAAG GCCCGCCTGG AAGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCCTAAA CCCTGCTCCG 
TTTTCTCCCC TCACCACACA ACAGATGAAG CCCGAAAAGC CTACCACCCT CCAGAGGCCC 
CAAGAAACCG TCATCCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACAGT TAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCT 
GACCCGTGGA AGCGCGACGC TAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CAGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAATTCC AGAAGCGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGACGA CGAGGAGGAG GAGGATGATG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGTTGCAGG ACGAGGAGCG GAGGCGACAG 
CAGCAGTTAG AAGAGATGCG TAAACGCGAA GCAGAAGACC GAGCAAGACA AGAGGAAGAG 
CGCCATCGGC AGGAGGAGGA GCGAACAAAA CGAGACGCTG AAGAAAAGAG GCGACAGGAA 
GAAGGGTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCATGACGA GGCGGCACGC 
AGGTTGTTGG AGCCCGAGGC GCCCGGTCTG TGCCGCCCTC CGCTTCCCCA GGACTACGAG 
CCCCAGTCCC CGTCCCCCAC GCCCAGCGCC CCTCCTCCCC CGCCTCAGCG AAACGCCTCC 
TACCTCAAAA CACAGGTCCT CTCCCCTGAC TCGCTGTTCA CTGCCAAGTT TGTTGCGTAC 
AATGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCGA ACTCTTACCC AGGATCTACG 
GGGGCAGCTG TTGGAACCCA TGAGGCGTAT CGGGATGCAA GAGAGAAGCG CTCTAAAAGC 
CAAGATGCAG ATGTCCCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAAGAACGC 
CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA 
ACAGAACTGG AGAATGAACT GAACACAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971546.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827035.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

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CloneID	OCb53671
Clone ID Related Accession (Same CDS sequence)	XM_017971546.1
Accession Version	XM_017971546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTCT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATTCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACTGACC CAGATTCAGG GCTGTGCTCA 
CATTACTGGG GCGCGATCAT CCGTCAGCAG CTGGGCCATA TTGAAGCATG GGCTGAGAAG 
CAGGGGCTGG AGCTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TTCAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGGCTGTGG CTGAAAACAC TGCTGATGAG 
CTGGCCCGCA GCGATGGAAG GGAGGTGCAG TTGGAGGAGG ATCCTGACCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCCCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCGGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC CGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGGCT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTC CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAG CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGATAGA CTGATGAAAA ACAGAGCTGA TCACCGCTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAGGTGCATA TGCCTCTGGA 
ACAGCAGCGA AGATAACATC TGTCTCTACT GGGAACCTCT GCACCGAGGA GCAGAGTCCT 
CCGCCGAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTCACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAC 
GAAGAAAAGC CACATACGCA CACAGACAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAACTTACC AGCTTGAGCG GCATCGAATA 
GAGGTGGCTC TAGAACGCAA GTCCGATAGT GATATGTGGG TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CATCTAGCCA AGAGCACCTG AACCATTCCT CTAAGCCGGT CACCCCTGCT 
TCCACGCTGA CCAAAAGCGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC CCTCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCC 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATC TGCCTCTCCC ACCACCTCCT 
TCTGCCAACC AGATAGGACT GCCGTCTGCG CAGGTGGCTG CTGCAGAACG GAAAAAGAGG 
GAAGAGCATC AGCGCTGGTA TGAGAAGGAA AAGGCCCGCC TGGAAGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCCT AAACCCTGCT 
CCGTTTTCTC CCCTCACCAC ACAACAGATG AAGCCCGAAA AGCCTACCAC CCTCCAGAGG 
CCCCAAGAAA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC AGTTAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCTGACCCGT GGAAGCGCGA CGCTAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCAGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAAT TCCAGAAGCG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA CGACGAGGAG GAGGAGGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGACAGCAG 
CAGTTAGAAG AGATGCGTAA ACGCGAAGCA GAAGACCGAG CAAGACAAGA GGAAGAGCGC 
CATCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ATGACGAGGC GGCACGCAGG 
TTGTTGGAGC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCAGGA CTACGAGCCC 
CAGTCCCCGT CCCCCACGCC CAGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCTGACTCG CTGTTCACTG CCAAGTTTGT TGCGTACAAT 
GAGGAGGAGG AGGAGGACTG CAGCCTAGCA GGACCGAACT CTTACCCAGG ATCTACGGGG 
GCAGCTGTTG GAACCCATGA GGCGTATCGG GATGCAAGAG AGAAGCGCTC TAAAAGCCAA 
GATGCAGATG TCCCTGGAAG TTCTGGGGCC CCTGAAAACT TGACATTCAA AGAACGCCAG 
CGTCTTTTTT CACAAGGTCA AGATGTATCC AATAAAGTGA AAGCTTCTCG TAAATTAACA 
GAACTGGAGA ATGAACTGAA CACAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827035.1
CDS	112..5601
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPQDYEPQSPSPTPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEDCSLAGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971546.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971546.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTCT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATTCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACTGACC CAGATTCAGG GCTGTGCTCA 
CATTACTGGG GCGCGATCAT CCGTCAGCAG CTGGGCCATA TTGAAGCATG GGCTGAGAAG 
CAGGGGCTGG AGCTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TTCAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGGCTGTGG CTGAAAACAC TGCTGATGAG 
CTGGCCCGCA GCGATGGAAG GGAGGTGCAG TTGGAGGAGG ATCCTGACCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCCCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCGGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC CGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGGCT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTC CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAG CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGATAGA CTGATGAAAA ACAGAGCTGA TCACCGCTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAGGTGCATA TGCCTCTGGA 
ACAGCAGCGA AGATAACATC TGTCTCTACT GGGAACCTCT GCACCGAGGA GCAGAGTCCT 
CCGCCGAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTCACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAC 
GAAGAAAAGC CACATACGCA CACAGACAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAACTTACC AGCTTGAGCG GCATCGAATA 
GAGGTGGCTC TAGAACGCAA GTCCGATAGT GATATGTGGG TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CATCTAGCCA AGAGCACCTG AACCATTCCT CTAAGCCGGT CACCCCTGCT 
TCCACGCTGA CCAAAAGCGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC CCTCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCC 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATC TGCCTCTCCC ACCACCTCCT 
TCTGCCAACC AGATAGGACT GCCGTCTGCG CAGGTGGCTG CTGCAGAACG GAAAAAGAGG 
GAAGAGCATC AGCGCTGGTA TGAGAAGGAA AAGGCCCGCC TGGAAGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCCT AAACCCTGCT 
CCGTTTTCTC CCCTCACCAC ACAACAGATG AAGCCCGAAA AGCCTACCAC CCTCCAGAGG 
CCCCAAGAAA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC AGTTAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCTGACCCGT GGAAGCGCGA CGCTAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCAGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAAT TCCAGAAGCG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA CGACGAGGAG GAGGAGGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGACAGCAG 
CAGTTAGAAG AGATGCGTAA ACGCGAAGCA GAAGACCGAG CAAGACAAGA GGAAGAGCGC 
CATCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ATGACGAGGC GGCACGCAGG 
TTGTTGGAGC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCAGGA CTACGAGCCC 
CAGTCCCCGT CCCCCACGCC CAGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCTGACTCG CTGTTCACTG CCAAGTTTGT TGCGTACAAT 
GAGGAGGAGG AGGAGGACTG CAGCCTAGCA GGACCGAACT CTTACCCAGG ATCTACGGGG 
GCAGCTGTTG GAACCCATGA GGCGTATCGG GATGCAAGAG AGAAGCGCTC TAAAAGCCAA 
GATGCAGATG TCCCTGGAAG TTCTGGGGCC CCTGAAAACT TGACATTCAA AGAACGCCAG 
CGTCTTTTTT CACAAGGTCA AGATGTATCC AATAAAGTGA AAGCTTCTCG TAAATTAACA 
GAACTGGAGA ATGAACTGAA CACAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971547.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827036.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

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CloneID	OCb53672
Clone ID Related Accession (Same CDS sequence)	XM_017971547.1
Accession Version	XM_017971547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTCTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGACGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATTCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACTGACCCAG ATTCAGGGCT GTGCTCACAT 
TACTGGGGCG CGATCATCCG TCAGCAGCTG GGCCATATTG AAGCATGGGC TGAGAAGCAG 
GGGCTGGAGC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTTC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG GCTGTGGCTG AAAACACTGC TGATGAGCTG 
GCCCGCAGCG ATGGAAGGGA GGTGCAGTTG GAGGAGGATC CTGACCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCCCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCGGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT CAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCCGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGGCTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTCCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAGCTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGATAGACTG ATGAAAAACA GAGCTGATCA CCGCTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAG GTGCATATGC CTCTGGAACA 
GCAGCGAAGA TAACATCTGT CTCTACTGGG AACCTCTGCA CCGAGGAGCA GAGTCCTCCG 
CCGAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTCACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTACGAA 
GAAAAGCCAC ATACGCACAC AGACAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA ACTTACCAGC TTGAGCGGCA TCGAATAGAG 
GTGGCTCTAG AACGCAAGTC CGATAGTGAT ATGTGGGTAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACAT CTAGCCAAGA GCACCTGAAC CATTCCTCTA AGCCGGTCAC CCCTGCTTCC 
ACGCTGACCA AAAGCGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCGTCT CCCAGCCCCT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCCGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATCTGC CTCTCCCACC ACCTCCTTCT 
GCCAACCAGA TAGGACTGCC GTCTGCGCAG GTGGCTGCTG CAGAACGGAA AAAGAGGGAA 
GAGCATCAGC GCTGGTATGA GAAGGAAAAG GCCCGCCTGG AAGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCCTAAA CCCTGCTCCG 
TTTTCTCCCC TCACCACACA ACAGATGAAG CCCGAAAAGC CTACCACCCT CCAGAGGCCC 
CAAGAAACCG TCATCCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACAGT TAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCT 
GACCCGTGGA AGCGCGACGC TAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CAGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAATTCC AGAAGCGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGACGA CGAGGAGGAG GAGGATGATG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGGACGAGG AGCGGAGGCG ACAGCAGCAG 
TTAGAAGAGA TGCGTAAACG CGAAGCAGAA GACCGAGCAA GACAAGAGGA AGAGCGCCAT 
CGGCAGGAGG AGGAGCGAAC AAAACGAGAC GCTGAAGAAA AGAGGCGACA GGAAGAAGGG 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCATG ACGAGGCGGC ACGCAGGTTG 
TTGGAGCCCG AGGCGCCCGG TCTGTGCCGC CCTCCGCTTC CCCAGGACTA CGAGCCCCAG 
TCCCCGTCCC CCACGCCCAG CGCCCCTCCT CCCCCGCCTC AGCGAAACGC CTCCTACCTC 
AAAACACAGG TCCTCTCCCC TGACTCGCTG TTCACTGCCA AGTTTGTTGC GTACAATGAG 
GAGGAGGAGG AGGACTGCAG CCTAGCAGGA CCGAACTCTT ACCCAGGATC TACGGGGGCA 
GCTGTTGGAA CCCATGAGGC GTATCGGGAT GCAAGAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATGTCC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAAGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827036.1
CDS	112..5598
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPQDYEPQSPSPTPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEDCSLAGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971547.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971547.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTCTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGACGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATTCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACTGACCCAG ATTCAGGGCT GTGCTCACAT 
TACTGGGGCG CGATCATCCG TCAGCAGCTG GGCCATATTG AAGCATGGGC TGAGAAGCAG 
GGGCTGGAGC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTTC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG GCTGTGGCTG AAAACACTGC TGATGAGCTG 
GCCCGCAGCG ATGGAAGGGA GGTGCAGTTG GAGGAGGATC CTGACCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCCCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCGGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT CAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCCGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGGCTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTCCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAGCTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGATAGACTG ATGAAAAACA GAGCTGATCA CCGCTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAG GTGCATATGC CTCTGGAACA 
GCAGCGAAGA TAACATCTGT CTCTACTGGG AACCTCTGCA CCGAGGAGCA GAGTCCTCCG 
CCGAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTCACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTACGAA 
GAAAAGCCAC ATACGCACAC AGACAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA ACTTACCAGC TTGAGCGGCA TCGAATAGAG 
GTGGCTCTAG AACGCAAGTC CGATAGTGAT ATGTGGGTAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACAT CTAGCCAAGA GCACCTGAAC CATTCCTCTA AGCCGGTCAC CCCTGCTTCC 
ACGCTGACCA AAAGCGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCGTCT CCCAGCCCCT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCCGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATCTGC CTCTCCCACC ACCTCCTTCT 
GCCAACCAGA TAGGACTGCC GTCTGCGCAG GTGGCTGCTG CAGAACGGAA AAAGAGGGAA 
GAGCATCAGC GCTGGTATGA GAAGGAAAAG GCCCGCCTGG AAGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCCTAAA CCCTGCTCCG 
TTTTCTCCCC TCACCACACA ACAGATGAAG CCCGAAAAGC CTACCACCCT CCAGAGGCCC 
CAAGAAACCG TCATCCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACAGT TAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCT 
GACCCGTGGA AGCGCGACGC TAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CAGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAATTCC AGAAGCGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGACGA CGAGGAGGAG GAGGATGATG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGGACGAGG AGCGGAGGCG ACAGCAGCAG 
TTAGAAGAGA TGCGTAAACG CGAAGCAGAA GACCGAGCAA GACAAGAGGA AGAGCGCCAT 
CGGCAGGAGG AGGAGCGAAC AAAACGAGAC GCTGAAGAAA AGAGGCGACA GGAAGAAGGG 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCATG ACGAGGCGGC ACGCAGGTTG 
TTGGAGCCCG AGGCGCCCGG TCTGTGCCGC CCTCCGCTTC CCCAGGACTA CGAGCCCCAG 
TCCCCGTCCC CCACGCCCAG CGCCCCTCCT CCCCCGCCTC AGCGAAACGC CTCCTACCTC 
AAAACACAGG TCCTCTCCCC TGACTCGCTG TTCACTGCCA AGTTTGTTGC GTACAATGAG 
GAGGAGGAGG AGGACTGCAG CCTAGCAGGA CCGAACTCTT ACCCAGGATC TACGGGGGCA 
GCTGTTGGAA CCCATGAGGC GTATCGGGAT GCAAGAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATGTCC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAAGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971548.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827037.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

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CloneID	OCb53673
Clone ID Related Accession (Same CDS sequence)	XM_017971548.1
Accession Version	XM_017971548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGAGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCGTTGCG ACAGGCATCT 
GATAAAGATG ATAGGCCTTT CCAAGGGGAG GACGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCTGA AACCAGCTTC ACACGAACAA TTTCTAATCC TGAGGTGGTC 
ATGAAACGGC GGAGGCAACA GAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCAG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCAGAAGCT 
TTAGAGAAGT ATGGCCTGGA AAAAGAAAAC CCGAAGGATT ACTGCATAGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CAAAGAAACA CTTGGAAGGG 
AAGCCACCCA AAGGAAAGGA GAAAGCCGAC GGGTCTGGCT ATGGCTCCAC CCTTCCCCCT 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTATGAAGA TGGTTCTGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTT 
CAATTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTATTT 
GGCCCAGGAA TTCAGCCTCA TCACTGTGAC CTCACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATAGACGC AGAAACCTAT GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGTTCT TGCAAAAAGA TCTGTGGATG GAGGCCTCAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTAGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCA 
TCCGCCTCCA GCACAGCCGA GCGGGGGATG GTGAAGCCAA TGATCAGAGT AGAGCAGCAA 
CCAGATTATC GCAGGCAAGA AAGCAGAACC CAGGATGCTT CTGGGCCTGA CCTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TCTTATCTGC GATTATAAAC 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCAACAGA GCGCACGCAC 
AAAGTCATTG CAGTTGTCAA CAAGATGGTG AGCATGATGG AAGGCGTCAT CCAGGAAGTA 
GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT 
GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGAAT CACATTGGAT 
GCCCAAGATG TTCTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT 
CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG 
CAAAGACCAA AAATAGATGA CGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA 
CGCTGCAGAG TCAATGCCGC CCTGACCATT CAGCTCTTCT CTCAGCTCTT CCACTTCATC 
AATATGTGGC TGTTCAATAG ATTGGTGACT GACCCAGATT CAGGGCTGTG CTCACATTAC 
TGGGGCGCGA TCATCCGTCA GCAGCTGGGC CATATTGAAG CATGGGCTGA GAAGCAGGGG 
CTGGAGCTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC 
ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT 
TCACTTCAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC 
CCAACGGATC TTATAGAAAA TGTAGTGGCT GTGGCTGAAA ACACTGCTGA TGAGCTGGCC 
CGCAGCGATG GAAGGGAGGT GCAGTTGGAG GAGGATCCTG ACCTGCAGCT GCCTTTTCTT 
TTGCCGGAAG ACGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA 
TTTTTAGACC CCCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA 
GGTACTTGGA CAATATATTT TGAAGGTGCG GATTATGAAA GTCACCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG 
CAGAATGGAA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA 
ATCTATGTGA AGTCCGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GGCTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTCCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAGCTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA TAGACTGATG AAAAACAGAG CTGATCACCG CTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAGGTG CATATGCCTC TGGAACAGCA 
GCGAAGATAA CATCTGTCTC TACTGGGAAC CTCTGCACCG AGGAGCAGAG TCCTCCGCCG 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAAGAA 
AAGCCACATA CGCACACAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAACT TACCAGCTTG AGCGGCATCG AATAGAGGTG 
GCTCTAGAAC GCAAGTCCGA TAGTGATATG TGGGTAAATC AGAGCTCCTC ACTGGACTCC 
AGTACATCTA GCCAAGAGCA CCTGAACCAT TCCTCTAAGC CGGTCACCCC TGCTTCCACG 
CTGACCAAAA GCGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCGTCTCCC AGCCCCTCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCCGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATCTGCCTC TCCCACCACC TCCTTCTGCC 
AACCAGATAG GACTGCCGTC TGCGCAGGTG GCTGCTGCAG AACGGAAAAA GAGGGAAGAG 
CATCAGCGCT GGTATGAGAA GGAAAAGGCC CGCCTGGAAG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCCTAAACCC TGCTCCGTTT 
TCTCCCCTCA CCACACAACA GATGAAGCCC GAAAAGCCTA CCACCCTCCA GAGGCCCCAA 
GAAACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACAGTTAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCTGAC 
CCGTGGAAGC GCGACGCTAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCAG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAATTCCAGA AGCGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGACGACGA GGAGGAGGAG GATGATGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG GACGAGGAGC GGAGGCGACA GCAGCAGTTA 
GAAGAGATGC GTAAACGCGA AGCAGAAGAC CGAGCAAGAC AAGAGGAAGA GCGCCATCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCATGACG AGGCGGCACG CAGGTTGTTG 
GAGCCCGAGG CGCCCGGTCT GTGCCGCCCT CCGCTTCCCC AGGACTACGA GCCCCAGTCC 
CCGTCCCCCA CGCCCAGCGC CCCTCCTCCC CCGCCTCAGC GAAACGCCTC CTACCTCAAA 
ACACAGGTCC TCTCCCCTGA CTCGCTGTTC ACTGCCAAGT TTGTTGCGTA CAATGAGGAG 
GAGGAGGAGG ACTGCAGCCT AGCAGGACCG AACTCTTACC CAGGATCTAC GGGGGCAGCT 
GTTGGAACCC ATGAGGCGTA TCGGGATGCA AGAGAGAAGC GCTCTAAAAG CCAAGATGCA 
GATGTCCCTG GAAGTTCTGG GGCCCCTGAA AACTTGACAT TCAAAGAACG CCAGCGTCTT 
TTTTCACAAG GTCAAGATGT ATCCAATAAA GTGAAAGCTT CTCGTAAATT AACAGAACTG 
GAGAATGAAC TGAACACAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827037.1
CDS	112..5595
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPQDYEPQSPSPTPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEDCSLAGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971548.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971548.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGAGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCGTTGCG ACAGGCATCT 
GATAAAGATG ATAGGCCTTT CCAAGGGGAG GACGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCTGA AACCAGCTTC ACACGAACAA TTTCTAATCC TGAGGTGGTC 
ATGAAACGGC GGAGGCAACA GAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCAG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCAGAAGCT 
TTAGAGAAGT ATGGCCTGGA AAAAGAAAAC CCGAAGGATT ACTGCATAGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CAAAGAAACA CTTGGAAGGG 
AAGCCACCCA AAGGAAAGGA GAAAGCCGAC GGGTCTGGCT ATGGCTCCAC CCTTCCCCCT 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTATGAAGA TGGTTCTGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTT 
CAATTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTATTT 
GGCCCAGGAA TTCAGCCTCA TCACTGTGAC CTCACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATAGACGC AGAAACCTAT GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGTTCT TGCAAAAAGA TCTGTGGATG GAGGCCTCAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTAGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCA 
TCCGCCTCCA GCACAGCCGA GCGGGGGATG GTGAAGCCAA TGATCAGAGT AGAGCAGCAA 
CCAGATTATC GCAGGCAAGA AAGCAGAACC CAGGATGCTT CTGGGCCTGA CCTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TCTTATCTGC GATTATAAAC 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCAACAGA GCGCACGCAC 
AAAGTCATTG CAGTTGTCAA CAAGATGGTG AGCATGATGG AAGGCGTCAT CCAGGAAGTA 
GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT 
GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGAAT CACATTGGAT 
GCCCAAGATG TTCTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT 
CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG 
CAAAGACCAA AAATAGATGA CGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA 
CGCTGCAGAG TCAATGCCGC CCTGACCATT CAGCTCTTCT CTCAGCTCTT CCACTTCATC 
AATATGTGGC TGTTCAATAG ATTGGTGACT GACCCAGATT CAGGGCTGTG CTCACATTAC 
TGGGGCGCGA TCATCCGTCA GCAGCTGGGC CATATTGAAG CATGGGCTGA GAAGCAGGGG 
CTGGAGCTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC 
ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT 
TCACTTCAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC 
CCAACGGATC TTATAGAAAA TGTAGTGGCT GTGGCTGAAA ACACTGCTGA TGAGCTGGCC 
CGCAGCGATG GAAGGGAGGT GCAGTTGGAG GAGGATCCTG ACCTGCAGCT GCCTTTTCTT 
TTGCCGGAAG ACGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA 
TTTTTAGACC CCCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA 
GGTACTTGGA CAATATATTT TGAAGGTGCG GATTATGAAA GTCACCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG 
CAGAATGGAA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA 
ATCTATGTGA AGTCCGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GGCTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTCCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAGCTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA TAGACTGATG AAAAACAGAG CTGATCACCG CTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAGGTG CATATGCCTC TGGAACAGCA 
GCGAAGATAA CATCTGTCTC TACTGGGAAC CTCTGCACCG AGGAGCAGAG TCCTCCGCCG 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAAGAA 
AAGCCACATA CGCACACAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAACT TACCAGCTTG AGCGGCATCG AATAGAGGTG 
GCTCTAGAAC GCAAGTCCGA TAGTGATATG TGGGTAAATC AGAGCTCCTC ACTGGACTCC 
AGTACATCTA GCCAAGAGCA CCTGAACCAT TCCTCTAAGC CGGTCACCCC TGCTTCCACG 
CTGACCAAAA GCGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCGTCTCCC AGCCCCTCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCCGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATCTGCCTC TCCCACCACC TCCTTCTGCC 
AACCAGATAG GACTGCCGTC TGCGCAGGTG GCTGCTGCAG AACGGAAAAA GAGGGAAGAG 
CATCAGCGCT GGTATGAGAA GGAAAAGGCC CGCCTGGAAG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCCTAAACCC TGCTCCGTTT 
TCTCCCCTCA CCACACAACA GATGAAGCCC GAAAAGCCTA CCACCCTCCA GAGGCCCCAA 
GAAACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACAGTTAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCTGAC 
CCGTGGAAGC GCGACGCTAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCAG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAATTCCAGA AGCGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGACGACGA GGAGGAGGAG GATGATGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG GACGAGGAGC GGAGGCGACA GCAGCAGTTA 
GAAGAGATGC GTAAACGCGA AGCAGAAGAC CGAGCAAGAC AAGAGGAAGA GCGCCATCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCATGACG AGGCGGCACG CAGGTTGTTG 
GAGCCCGAGG CGCCCGGTCT GTGCCGCCCT CCGCTTCCCC AGGACTACGA GCCCCAGTCC 
CCGTCCCCCA CGCCCAGCGC CCCTCCTCCC CCGCCTCAGC GAAACGCCTC CTACCTCAAA 
ACACAGGTCC TCTCCCCTGA CTCGCTGTTC ACTGCCAAGT TTGTTGCGTA CAATGAGGAG 
GAGGAGGAGG ACTGCAGCCT AGCAGGACCG AACTCTTACC CAGGATCTAC GGGGGCAGCT 
GTTGGAACCC ATGAGGCGTA TCGGGATGCA AGAGAGAAGC GCTCTAAAAG CCAAGATGCA 
GATGTCCCTG GAAGTTCTGG GGCCCCTGAA AACTTGACAT TCAAAGAACG CCAGCGTCTT 
TTTTCACAAG GTCAAGATGT ATCCAATAAA GTGAAAGCTT CTCGTAAATT AACAGAACTG 
GAGAATGAAC TGAACACAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971549.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827038.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

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CloneID	OCb53674
Clone ID Related Accession (Same CDS sequence)	XM_017971549.1
Accession Version	XM_017971549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTCTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GACGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TTCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CTGACCCAGA TTCAGGGCTG TGCTCACATT ACTGGGGCGC GATCATCCGT 
CAGCAGCTGG GCCATATTGA AGCATGGGCT GAGAAGCAGG GGCTGGAGCT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTTCA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGG CTGTGGCTGA AAACACTGCT GATGAGCTGG CCCGCAGCGA TGGAAGGGAG 
GTGCAGTTGG AGGAGGATCC TGACCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCCCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CGGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTC 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCCGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGGCTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTCCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAGCTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GATAGACTGA TGAAAAACAG AGCTGATCAC CGCTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAGG TGCATATGCC TCTGGAACAG CAGCGAAGAT AACATCTGTC 
TCTACTGGGA ACCTCTGCAC CGAGGAGCAG AGTCCTCCGC CGAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTCACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTACGAAG AAAAGCCACA TACGCACACA 
GACAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAA CTTACCAGCT TGAGCGGCAT CGAATAGAGG TGGCTCTAGA ACGCAAGTCC 
GATAGTGATA TGTGGGTAAA TCAGAGCTCC TCACTGGACT CCAGTACATC TAGCCAAGAG 
CACCTGAACC ATTCCTCTAA GCCGGTCACC CCTGCTTCCA CGCTGACCAA AAGCGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCCCTC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCCGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATCTGCC TCTCCCACCA CCTCCTTCTG CCAACCAGAT AGGACTGCCG 
TCTGCGCAGG TGGCTGCTGC AGAACGGAAA AAGAGGGAAG AGCATCAGCG CTGGTATGAG 
AAGGAAAAGG CCCGCCTGGA AGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCCTAAAC CCTGCTCCGT TTTCTCCCCT CACCACACAA 
CAGATGAAGC CCGAAAAGCC TACCACCCTC CAGAGGCCCC AAGAAACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACAGTT 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCTG ACCCGTGGAA GCGCGACGCT 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC AGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAATTCCA GAAGCGACTC CAGGAGTCGA AGCAGAAGGA CGAAGACGAC 
GAGGAGGAGG AGGATGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGACAGC AGCAGTTAGA AGAGATGCGT 
AAACGCGAAG CAGAAGACCG AGCAAGACAA GAGGAAGAGC GCCATCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCATGACGAG GCGGCACGCA GGTTGTTGGA GCCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCAG GACTACGAGC CCCAGTCCCC GTCCCCCACG 
CCCAGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCTGACT CGCTGTTCAC TGCCAAGTTT GTTGCGTACA ATGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGACCGAA CTCTTACCCA GGATCTACGG GGGCAGCTGT TGGAACCCAT 
GAGGCGTATC GGGATGCAAG AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TGTCCCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAAGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827038.1
CDS	112..5583
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPQDYEPQSPSPTPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEDCSLAGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971549.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971549.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTCTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GACGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TTCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CTGACCCAGA TTCAGGGCTG TGCTCACATT ACTGGGGCGC GATCATCCGT 
CAGCAGCTGG GCCATATTGA AGCATGGGCT GAGAAGCAGG GGCTGGAGCT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTTCA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGG CTGTGGCTGA AAACACTGCT GATGAGCTGG CCCGCAGCGA TGGAAGGGAG 
GTGCAGTTGG AGGAGGATCC TGACCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCCCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CGGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTC 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCCGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGGCTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTCCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAGCTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GATAGACTGA TGAAAAACAG AGCTGATCAC CGCTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAGG TGCATATGCC TCTGGAACAG CAGCGAAGAT AACATCTGTC 
TCTACTGGGA ACCTCTGCAC CGAGGAGCAG AGTCCTCCGC CGAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTCACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTACGAAG AAAAGCCACA TACGCACACA 
GACAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAA CTTACCAGCT TGAGCGGCAT CGAATAGAGG TGGCTCTAGA ACGCAAGTCC 
GATAGTGATA TGTGGGTAAA TCAGAGCTCC TCACTGGACT CCAGTACATC TAGCCAAGAG 
CACCTGAACC ATTCCTCTAA GCCGGTCACC CCTGCTTCCA CGCTGACCAA AAGCGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCCCTC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCCGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATCTGCC TCTCCCACCA CCTCCTTCTG CCAACCAGAT AGGACTGCCG 
TCTGCGCAGG TGGCTGCTGC AGAACGGAAA AAGAGGGAAG AGCATCAGCG CTGGTATGAG 
AAGGAAAAGG CCCGCCTGGA AGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCCTAAAC CCTGCTCCGT TTTCTCCCCT CACCACACAA 
CAGATGAAGC CCGAAAAGCC TACCACCCTC CAGAGGCCCC AAGAAACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACAGTT 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCTG ACCCGTGGAA GCGCGACGCT 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC AGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAATTCCA GAAGCGACTC CAGGAGTCGA AGCAGAAGGA CGAAGACGAC 
GAGGAGGAGG AGGATGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGACAGC AGCAGTTAGA AGAGATGCGT 
AAACGCGAAG CAGAAGACCG AGCAAGACAA GAGGAAGAGC GCCATCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCATGACGAG GCGGCACGCA GGTTGTTGGA GCCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCAG GACTACGAGC CCCAGTCCCC GTCCCCCACG 
CCCAGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCTGACT CGCTGTTCAC TGCCAAGTTT GTTGCGTACA ATGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGACCGAA CTCTTACCCA GGATCTACGG GGGCAGCTGT TGGAACCCAT 
GAGGCGTATC GGGATGCAAG AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TGTCCCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAAGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971550.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827039.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

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CloneID	OCb53675
Clone ID Related Accession (Same CDS sequence)	XM_017971550.1
Accession Version	XM_017971550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCCAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAATTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTATT TGGCCCAGGA ATTCAGCCTC ATCACTGTGA CCTCACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATAGACG CAGAAACCTA TGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGTTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTCA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC ATCCGCCTCC AGCACAGCCG AGCGGGGGAT GGTGAAGCCA 
ATGATCAGAG TAGAGCAGCA ACCAGATTAT CGCAGGCAAG AAAGCAGAAC CCAGGATGCT 
TCTGGGCCTG ACCTGATACT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTCTTATCTG CGATTATAAA CTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCAACAG AGCGCACGCA CAAAGTCATT GCAGTTGTCA ACAAGATGGT GAGCATGATG 
GAAGGCGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGAA TCACATTGGA TGCCCAAGAT GTTCTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAAAGACCA AAAATAGATG ACGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT TCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC TGACCCAGAT 
TCAGGGCTGT GCTCACATTA CTGGGGCGCG ATCATCCGTC AGCAGCTGGG CCATATTGAA 
GCATGGGCTG AGAAGCAGGG GCTGGAGCTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCACTTCAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGGC TGTGGCTGAA 
AACACTGCTG ATGAGCTGGC CCGCAGCGAT GGAAGGGAGG TGCAGTTGGA GGAGGATCCT 
GACCTGCAGC TGCCTTTTCT TTTGCCGGAA GACGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCCCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC GGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTCA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCCGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGGCTCTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTCCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAGCTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ATAGACTGAT GAAAAACAGA 
GCTGATCACC GCTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAGGT 
GCATATGCCT CTGGAACAGC AGCGAAGATA ACATCTGTCT CTACTGGGAA CCTCTGCACC 
GAGGAGCAGA GTCCTCCGCC GAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TCACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTACGAAGA AAAGCCACAT ACGCACACAG ACAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAAC TTACCAGCTT 
GAGCGGCATC GAATAGAGGT GGCTCTAGAA CGCAAGTCCG ATAGTGATAT GTGGGTAAAT 
CAGAGCTCCT CACTGGACTC CAGTACATCT AGCCAAGAGC ACCTGAACCA TTCCTCTAAG 
CCGGTCACCC CTGCTTCCAC GCTGACCAAA AGCGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCCCTCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCCGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATCTGCCT 
CTCCCACCAC CTCCTTCTGC CAACCAGATA GGACTGCCGT CTGCGCAGGT GGCTGCTGCA 
GAACGGAAAA AGAGGGAAGA GCATCAGCGC TGGTATGAGA AGGAAAAGGC CCGCCTGGAA 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCCTAAACC CTGCTCCGTT TTCTCCCCTC ACCACACAAC AGATGAAGCC CGAAAAGCCT 
ACCACCCTCC AGAGGCCCCA AGAAACCGTC ATCCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACCATCG AGCGCAGGGA CCTGCAGTAC ATCACAGTTA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCTGA CCCGTGGAAG CGCGACGCTA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCA 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAATTCCAG 
AAGCGACTCC AGGAGTCGAA GCAGAAGGAC GAAGACGACG AGGAGGAGGA GGATGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GGACGAGGAG 
CGGAGGCGAC AGCAGCAGTT AGAAGAGATG CGTAAACGCG AAGCAGAAGA CCGAGCAAGA 
CAAGAGGAAG AGCGCCATCG GCAGGAGGAG GAGCGAACAA AACGAGACGC TGAAGAAAAG 
AGGCGACAGG AAGAAGGGTA TTACAGCCGC CTGGAAGCCG AGAGGCGCAG ACAGCATGAC 
GAGGCGGCAC GCAGGTTGTT GGAGCCCGAG GCGCCCGGTC TGTGCCGCCC TCCGCTTCCC 
CAGGACTACG AGCCCCAGTC CCCGTCCCCC ACGCCCAGCG CCCCTCCTCC CCCGCCTCAG 
CGAAACGCCT CCTACCTCAA AACACAGGTC CTCTCCCCTG ACTCGCTGTT CACTGCCAAG 
TTTGTTGCGT ACAATGAGGA GGAGGAGGAG GACTGCAGCC TAGCAGGACC GAACTCTTAC 
CCAGGATCTA CGGGGGCAGC TGTTGGAACC CATGAGGCGT ATCGGGATGC AAGAGAGAAG 
CGCTCTAAAA GCCAAGATGC AGATGTCCCT GGAAGTTCTG GGGCCCCTGA AAACTTGACA 
TTCAAAGAAC GCCAGCGTCT TTTTTCACAA GGTCAAGATG TATCCAATAA AGTGAAAGCT 
TCTCGTAAAT TAACAGAACT GGAGAATGAA CTGAACACAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827039.1
CDS	112..5556
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPQDYEPQSPSPTPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEDCSLAGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971550.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971550.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCCAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAATTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTATT TGGCCCAGGA ATTCAGCCTC ATCACTGTGA CCTCACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATAGACG CAGAAACCTA TGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGTTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTCA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC ATCCGCCTCC AGCACAGCCG AGCGGGGGAT GGTGAAGCCA 
ATGATCAGAG TAGAGCAGCA ACCAGATTAT CGCAGGCAAG AAAGCAGAAC CCAGGATGCT 
TCTGGGCCTG ACCTGATACT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTCTTATCTG CGATTATAAA CTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCAACAG AGCGCACGCA CAAAGTCATT GCAGTTGTCA ACAAGATGGT GAGCATGATG 
GAAGGCGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGAA TCACATTGGA TGCCCAAGAT GTTCTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAAAGACCA AAAATAGATG ACGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT TCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC TGACCCAGAT 
TCAGGGCTGT GCTCACATTA CTGGGGCGCG ATCATCCGTC AGCAGCTGGG CCATATTGAA 
GCATGGGCTG AGAAGCAGGG GCTGGAGCTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCACTTCAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGGC TGTGGCTGAA 
AACACTGCTG ATGAGCTGGC CCGCAGCGAT GGAAGGGAGG TGCAGTTGGA GGAGGATCCT 
GACCTGCAGC TGCCTTTTCT TTTGCCGGAA GACGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCCCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC GGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTCA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCCGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGGCTCTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTCCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAGCTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ATAGACTGAT GAAAAACAGA 
GCTGATCACC GCTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAGGT 
GCATATGCCT CTGGAACAGC AGCGAAGATA ACATCTGTCT CTACTGGGAA CCTCTGCACC 
GAGGAGCAGA GTCCTCCGCC GAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TCACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTACGAAGA AAAGCCACAT ACGCACACAG ACAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAAC TTACCAGCTT 
GAGCGGCATC GAATAGAGGT GGCTCTAGAA CGCAAGTCCG ATAGTGATAT GTGGGTAAAT 
CAGAGCTCCT CACTGGACTC CAGTACATCT AGCCAAGAGC ACCTGAACCA TTCCTCTAAG 
CCGGTCACCC CTGCTTCCAC GCTGACCAAA AGCGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCGTCTCC CAGCCCCTCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCCGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATCTGCCT 
CTCCCACCAC CTCCTTCTGC CAACCAGATA GGACTGCCGT CTGCGCAGGT GGCTGCTGCA 
GAACGGAAAA AGAGGGAAGA GCATCAGCGC TGGTATGAGA AGGAAAAGGC CCGCCTGGAA 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCCTAAACC CTGCTCCGTT TTCTCCCCTC ACCACACAAC AGATGAAGCC CGAAAAGCCT 
ACCACCCTCC AGAGGCCCCA AGAAACCGTC ATCCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACCATCG AGCGCAGGGA CCTGCAGTAC ATCACAGTTA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCTGA CCCGTGGAAG CGCGACGCTA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCA 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAATTCCAG 
AAGCGACTCC AGGAGTCGAA GCAGAAGGAC GAAGACGACG AGGAGGAGGA GGATGATGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GGACGAGGAG 
CGGAGGCGAC AGCAGCAGTT AGAAGAGATG CGTAAACGCG AAGCAGAAGA CCGAGCAAGA 
CAAGAGGAAG AGCGCCATCG GCAGGAGGAG GAGCGAACAA AACGAGACGC TGAAGAAAAG 
AGGCGACAGG AAGAAGGGTA TTACAGCCGC CTGGAAGCCG AGAGGCGCAG ACAGCATGAC 
GAGGCGGCAC GCAGGTTGTT GGAGCCCGAG GCGCCCGGTC TGTGCCGCCC TCCGCTTCCC 
CAGGACTACG AGCCCCAGTC CCCGTCCCCC ACGCCCAGCG CCCCTCCTCC CCCGCCTCAG 
CGAAACGCCT CCTACCTCAA AACACAGGTC CTCTCCCCTG ACTCGCTGTT CACTGCCAAG 
TTTGTTGCGT ACAATGAGGA GGAGGAGGAG GACTGCAGCC TAGCAGGACC GAACTCTTAC 
CCAGGATCTA CGGGGGCAGC TGTTGGAACC CATGAGGCGT ATCGGGATGC AAGAGAGAAG 
CGCTCTAAAA GCCAAGATGC AGATGTCCCT GGAAGTTCTG GGGCCCCTGA AAACTTGACA 
TTCAAAGAAC GCCAGCGTCT TTTTTCACAA GGTCAAGATG TATCCAATAA AGTGAAAGCT 
TCTCGTAAAT TAACAGAACT GGAGAATGAA CTGAACACAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971551.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827040.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

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CloneID	OCb53676
Clone ID Related Accession (Same CDS sequence)	XM_017971551.1
Accession Version	XM_017971551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGAGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCGTTGCG ACAGGCATCT 
GATAAAGATG ATAGGCCTTT CCAAGGGGAG GACGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCTGA AACCAGCTTC ACACGAACAA TTTCTAATCC TGAGGTGGTC 
ATGAAACGGC GGAGGCAACA GAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCAG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCAGAAGCT 
TTAGAGAAGT ATGGCCTGGA AAAAGAAAAC CCGAAGGATT ACTGCATAGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CAAAGAAACA CTTGGAAGGG 
AAGCCACCCA AAGGAAAGGA GAAAGCCGAC GGGTCTGGCT ATGGCTCCAC CCTTCCCCCT 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTTCAATT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TATTTGGCCC AGGAATTCAG CCTCATCACT GTGACCTCAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATA GACGCAGAAA CCTATGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GTTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTCATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCATCCGC CTCCAGCACA GCCGAGCGGG GGATGGTGAA GCCAATGATC 
AGAGTAGAGC AGCAACCAGA TTATCGCAGG CAAGAAAGCA GAACCCAGGA TGCTTCTGGG 
CCTGACCTGA TACTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTCTTA 
TCTGCGATTA TAAACTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCA 
ACAGAGCGCA CGCACAAAGT CATTGCAGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GAATCACATT GGATGCCCAA 
GATGTTCTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGACGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATTCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACTGACCCA GATTCAGGGC TGTGCTCACA TTACTGGGGC 
GCGATCATCC GTCAGCAGCT GGGCCATATT GAAGCATGGG CTGAGAAGCA GGGGCTGGAG 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTT 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GGCTGTGGCT GAAAACACTG CTGATGAGCT GGCCCGCAGC 
GATGGAAGGG AGGTGCAGTT GGAGGAGGAT CCTGACCTGC AGCTGCCTTT TCTTTTGCCG 
GAAGACGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCCCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCGGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TCAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCCG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGGCTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTCCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAGCT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGATAGACT GATGAAAAAC AGAGCTGATC ACCGCTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA GGTGCATATG CCTCTGGAAC AGCAGCGAAG 
ATAACATCTG TCTCTACTGG GAACCTCTGC ACCGAGGAGC AGAGTCCTCC GCCGAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTCACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTACGA AGAAAAGCCA 
CATACGCACA CAGACAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AACTTACCAG CTTGAGCGGC ATCGAATAGA GGTGGCTCTA 
GAACGCAAGT CCGATAGTGA TATGTGGGTA AATCAGAGCT CCTCACTGGA CTCCAGTACA 
TCTAGCCAAG AGCACCTGAA CCATTCCTCT AAGCCGGTCA CCCCTGCTTC CACGCTGACC 
AAAAGCGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC 
TCCCAGCCCC TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCCGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATCTG CCTCTCCCAC CACCTCCTTC TGCCAACCAG 
ATAGGACTGC CGTCTGCGCA GGTGGCTGCT GCAGAACGGA AAAAGAGGGA AGAGCATCAG 
CGCTGGTATG AGAAGGAAAA GGCCCGCCTG GAAGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCCTAA ACCCTGCTCC GTTTTCTCCC 
CTCACCACAC AACAGATGAA GCCCGAAAAG CCTACCACCC TCCAGAGGCC CCAAGAAACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACAG TTAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC TGACCCGTGG 
AAGCGCGACG CTAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCAGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAATTC CAGAAGCGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGACG ACGAGGAGGA GGAGGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGGACGAG GAGCGGAGGC GACAGCAGCA GTTAGAAGAG 
ATGCGTAAAC GCGAAGCAGA AGACCGAGCA AGACAAGAGG AAGAGCGCCA TCGGCAGGAG 
GAGGAGCGAA CAAAACGAGA CGCTGAAGAA AAGAGGCGAC AGGAAGAAGG GTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAT GACGAGGCGG CACGCAGGTT GTTGGAGCCC 
GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT CCCCAGGACT ACGAGCCCCA GTCCCCGTCC 
CCCACGCCCA GCGCCCCTCC TCCCCCGCCT CAGCGAAACG CCTCCTACCT CAAAACACAG 
GTCCTCTCCC CTGACTCGCT GTTCACTGCC AAGTTTGTTG CGTACAATGA GGAGGAGGAG 
GAGGACTGCA GCCTAGCAGG ACCGAACTCT TACCCAGGAT CTACGGGGGC AGCTGTTGGA 
ACCCATGAGG CGTATCGGGA TGCAAGAGAG AAGCGCTCTA AAAGCCAAGA TGCAGATGTC 
CCTGGAAGTT CTGGGGCCCC TGAAAACTTG ACATTCAAAG AACGCCAGCG TCTTTTTTCA 
CAAGGTCAAG ATGTATCCAA TAAAGTGAAA GCTTCTCGTA AATTAACAGA ACTGGAGAAT 
GAACTGAACA CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827040.1
CDS	112..5529
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPQDYEPQSPSPTPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEDCSLAGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971551.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971551.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGAGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCGTTGCG ACAGGCATCT 
GATAAAGATG ATAGGCCTTT CCAAGGGGAG GACGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCTGA AACCAGCTTC ACACGAACAA TTTCTAATCC TGAGGTGGTC 
ATGAAACGGC GGAGGCAACA GAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCAG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCAGAAGCT 
TTAGAGAAGT ATGGCCTGGA AAAAGAAAAC CCGAAGGATT ACTGCATAGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CAAAGAAACA CTTGGAAGGG 
AAGCCACCCA AAGGAAAGGA GAAAGCCGAC GGGTCTGGCT ATGGCTCCAC CCTTCCCCCT 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTTCAATT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TATTTGGCCC AGGAATTCAG CCTCATCACT GTGACCTCAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATA GACGCAGAAA CCTATGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GTTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTCATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCATCCGC CTCCAGCACA GCCGAGCGGG GGATGGTGAA GCCAATGATC 
AGAGTAGAGC AGCAACCAGA TTATCGCAGG CAAGAAAGCA GAACCCAGGA TGCTTCTGGG 
CCTGACCTGA TACTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTCTTA 
TCTGCGATTA TAAACTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCA 
ACAGAGCGCA CGCACAAAGT CATTGCAGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GAATCACATT GGATGCCCAA 
GATGTTCTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGACGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATTCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACTGACCCA GATTCAGGGC TGTGCTCACA TTACTGGGGC 
GCGATCATCC GTCAGCAGCT GGGCCATATT GAAGCATGGG CTGAGAAGCA GGGGCTGGAG 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTT 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GGCTGTGGCT GAAAACACTG CTGATGAGCT GGCCCGCAGC 
GATGGAAGGG AGGTGCAGTT GGAGGAGGAT CCTGACCTGC AGCTGCCTTT TCTTTTGCCG 
GAAGACGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCCCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCGGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TCAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCCG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGGCTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTCCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAGCT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGATAGACT GATGAAAAAC AGAGCTGATC ACCGCTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA GGTGCATATG CCTCTGGAAC AGCAGCGAAG 
ATAACATCTG TCTCTACTGG GAACCTCTGC ACCGAGGAGC AGAGTCCTCC GCCGAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTCACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTACGA AGAAAAGCCA 
CATACGCACA CAGACAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AACTTACCAG CTTGAGCGGC ATCGAATAGA GGTGGCTCTA 
GAACGCAAGT CCGATAGTGA TATGTGGGTA AATCAGAGCT CCTCACTGGA CTCCAGTACA 
TCTAGCCAAG AGCACCTGAA CCATTCCTCT AAGCCGGTCA CCCCTGCTTC CACGCTGACC 
AAAAGCGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC 
TCCCAGCCCC TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCCGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATCTG CCTCTCCCAC CACCTCCTTC TGCCAACCAG 
ATAGGACTGC CGTCTGCGCA GGTGGCTGCT GCAGAACGGA AAAAGAGGGA AGAGCATCAG 
CGCTGGTATG AGAAGGAAAA GGCCCGCCTG GAAGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCCTAA ACCCTGCTCC GTTTTCTCCC 
CTCACCACAC AACAGATGAA GCCCGAAAAG CCTACCACCC TCCAGAGGCC CCAAGAAACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACAG TTAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC TGACCCGTGG 
AAGCGCGACG CTAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCAGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAATTC CAGAAGCGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGACG ACGAGGAGGA GGAGGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGGACGAG GAGCGGAGGC GACAGCAGCA GTTAGAAGAG 
ATGCGTAAAC GCGAAGCAGA AGACCGAGCA AGACAAGAGG AAGAGCGCCA TCGGCAGGAG 
GAGGAGCGAA CAAAACGAGA CGCTGAAGAA AAGAGGCGAC AGGAAGAAGG GTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAT GACGAGGCGG CACGCAGGTT GTTGGAGCCC 
GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT CCCCAGGACT ACGAGCCCCA GTCCCCGTCC 
CCCACGCCCA GCGCCCCTCC TCCCCCGCCT CAGCGAAACG CCTCCTACCT CAAAACACAG 
GTCCTCTCCC CTGACTCGCT GTTCACTGCC AAGTTTGTTG CGTACAATGA GGAGGAGGAG 
GAGGACTGCA GCCTAGCAGG ACCGAACTCT TACCCAGGAT CTACGGGGGC AGCTGTTGGA 
ACCCATGAGG CGTATCGGGA TGCAAGAGAG AAGCGCTCTA AAAGCCAAGA TGCAGATGTC 
CCTGGAAGTT CTGGGGCCCC TGAAAACTTG ACATTCAAAG AACGCCAGCG TCTTTTTTCA 
CAAGGTCAAG ATGTATCCAA TAAAGTGAAA GCTTCTCGTA AATTAACAGA ACTGGAGAAT 
GAACTGAACA CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971552.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827041.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

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CloneID	OCb53677
Clone ID Related Accession (Same CDS sequence)	XM_017971552.1
Accession Version	XM_017971552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTCT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATTCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACTGACC CAGATTCAGG GCTGTGCTCA 
CATTACTGGG GCGCGATCAT CCGTCAGCAG CTGGGCCATA TTGAAGCATG GGCTGAGAAG 
CAGGGGCTGG AGCTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TTCAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGGCTGTGG CTGAAAACAC TGCTGATGAG 
CTGGCCCGCA GCGATGGAAG GGAGGTGCAG TTGGAGGAGG ATCCTGACCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCCCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCGGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC CGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGGCT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTC CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAG CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGATAGA CTGATGAAAA ACAGAGCTGA TCACCGCTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAGGTGCATA TGCCTCTGGA 
ACAGCAGCGA AGATAACATC TGTCTCTACT GGGAACCTCT GCACCGAGGA GCAGAGTCCT 
CCGCCGAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTCACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAC 
GAAGAAAAGC CACATACGCA CACAGACAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAACTTACC AGCTTGAGCG GCATCGAATA 
GAGGTGGCTC TAGAACGCAA GTCCGATAGT GATATGTGGG TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CATCTAGCCA AGAGCACCTG AACCATTCCT CTAAGCCGGT CACCCCTGCT 
TCCACGCTGA CCAAAAGCGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC CCTCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCC 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATC TGCCTCTCCC ACCACCTCCT 
TCTGCCAACC AGATAGGACT GCCGTCTGCG CAGGTGGCTG CTGCAGAACG GAAAAAGAGG 
GAAGAGCATC AGCGCTGGTA TGAGAAGGAA AAGGCCCGCC TGGAAGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCCT AAACCCTGCT 
CCGTTTTCTC CCCTCACCAC ACAACAGATG AAGCCCGAAA AGCCTACCAC CCTCCAGAGG 
CCCCAAGAAA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC AGTTAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCTGACCCGT GGAAGCGCGA CGCTAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCAGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAAT TCCAGAAGCG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA CGACGAGGAG GAGGAGGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGACAGC AGCAGTTAGA AGAGATGCGT 
AAACGCGAAG CAGAAGACCG AGCAAGACAA GAGGAAGAGC GCCATCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCATGACGAG GCGGCACGCA GGTTGTTGGA GCCCGAGGCG 
CCCGGACCGA ACTCTTACCC AGGATCTACG GGGGCAGCTG TTGGAACCCA TGAGGCGTAT 
CGGGATGCAA GAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATGTCCCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAAGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827041.1
CDS	112..5454
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971552.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971552.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTCT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATTCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACTGACC CAGATTCAGG GCTGTGCTCA 
CATTACTGGG GCGCGATCAT CCGTCAGCAG CTGGGCCATA TTGAAGCATG GGCTGAGAAG 
CAGGGGCTGG AGCTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TTCAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGGCTGTGG CTGAAAACAC TGCTGATGAG 
CTGGCCCGCA GCGATGGAAG GGAGGTGCAG TTGGAGGAGG ATCCTGACCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCCCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCGGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC CGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGGCT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTC CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAG CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGATAGA CTGATGAAAA ACAGAGCTGA TCACCGCTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAGGTGCATA TGCCTCTGGA 
ACAGCAGCGA AGATAACATC TGTCTCTACT GGGAACCTCT GCACCGAGGA GCAGAGTCCT 
CCGCCGAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTCACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAC 
GAAGAAAAGC CACATACGCA CACAGACAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAACTTACC AGCTTGAGCG GCATCGAATA 
GAGGTGGCTC TAGAACGCAA GTCCGATAGT GATATGTGGG TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CATCTAGCCA AGAGCACCTG AACCATTCCT CTAAGCCGGT CACCCCTGCT 
TCCACGCTGA CCAAAAGCGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC CCTCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCC 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATC TGCCTCTCCC ACCACCTCCT 
TCTGCCAACC AGATAGGACT GCCGTCTGCG CAGGTGGCTG CTGCAGAACG GAAAAAGAGG 
GAAGAGCATC AGCGCTGGTA TGAGAAGGAA AAGGCCCGCC TGGAAGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCCT AAACCCTGCT 
CCGTTTTCTC CCCTCACCAC ACAACAGATG AAGCCCGAAA AGCCTACCAC CCTCCAGAGG 
CCCCAAGAAA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC AGTTAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCTGACCCGT GGAAGCGCGA CGCTAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCAGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAAT TCCAGAAGCG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA CGACGAGGAG GAGGAGGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGACAGC AGCAGTTAGA AGAGATGCGT 
AAACGCGAAG CAGAAGACCG AGCAAGACAA GAGGAAGAGC GCCATCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCATGACGAG GCGGCACGCA GGTTGTTGGA GCCCGAGGCG 
CCCGGACCGA ACTCTTACCC AGGATCTACG GGGGCAGCTG TTGGAACCCA TGAGGCGTAT 
CGGGATGCAA GAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATGTCCCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAAGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971553.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827042.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

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CloneID	OCb53678
Clone ID Related Accession (Same CDS sequence)	XM_017971553.1
Accession Version	XM_017971553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTCT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATTCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACTGACC CAGATTCAGG GCTGTGCTCA 
CATTACTGGG GCGCGATCAT CCGTCAGCAG CTGGGCCATA TTGAAGCATG GGCTGAGAAG 
CAGGGGCTGG AGCTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TTCAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGGCTGTGG CTGAAAACAC TGCTGATGAG 
CTGGCCCGCA GCGATGGAAG GGAGGTGCAG TTGGAGGAGG ATCCTGACCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCCCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCGGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC CGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGGCT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTC CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAG CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGATAGA CTGATGAAAA ACAGAGCTGA TCACCGCTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAGGTGCATA TGCCTCTGGA 
ACAGCAGCGA AGATAACATC TGTCTCTACT GGGAACCTCT GCACCGAGGA GCAGAGTCCT 
CCGCCGAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTCACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAC 
GAAGAAAAGC CACATACGCA CACAGACAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAACTTACC AGCTTGAGCG GCATCGAATA 
GAGGTGGCTC TAGAACGCAA GTCCGATAGT GATATGTGGG TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CATCTAGCCA AGAGCACCTG AACCATTCCT CTAAGCCGGT CACCCCTGCT 
TCCACGCTGA CCAAAAGCGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC CCTCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCC 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATC TGCCTCTCCC ACCACCTCCT 
TCTGCCAACC AGATAGGACT GCCGTCTGCG CAGGTGGCTG CTGCAGAACG GAAAAAGAGG 
GAAGAGCATC AGCGCTGGTA TGAGAAGGAA AAGGCCCGCC TGGAAGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCCT AAACCCTGCT 
CCGTTTTCTC CCCTCACCAC ACAACAGATG AAGCCCGAAA AGCCTACCAC CCTCCAGAGG 
CCCCAAGAAA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC AGTTAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCTGACCCGT GGAAGCGCGA CGCTAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCAGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAAT TCCAGAAGCG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA CGACGAGGAG GAGGAGGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGA 
CAGCAGCAGT TAGAAGAGAT GCGTAAACGC GAAGCAGAAG ACCGAGCAAG ACAAGAGGAA 
GAGCGCCATC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCATGA CGAGGCGGCA 
CGCAGGTTGT TGGAGCCCGA GGCGCCCGGA CCGAACTCTT ACCCAGGATC TACGGGGGCA 
GCTGTTGGAA CCCATGAGGC GTATCGGGAT GCAAGAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATGTCC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAAGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827042.1
CDS	112..5418
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971553.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971553.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTCT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATTCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACTGACC CAGATTCAGG GCTGTGCTCA 
CATTACTGGG GCGCGATCAT CCGTCAGCAG CTGGGCCATA TTGAAGCATG GGCTGAGAAG 
CAGGGGCTGG AGCTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TTCAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGGCTGTGG CTGAAAACAC TGCTGATGAG 
CTGGCCCGCA GCGATGGAAG GGAGGTGCAG TTGGAGGAGG ATCCTGACCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCCCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCGGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC CGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGGCT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTC CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAG CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGATAGA CTGATGAAAA ACAGAGCTGA TCACCGCTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAGGTGCATA TGCCTCTGGA 
ACAGCAGCGA AGATAACATC TGTCTCTACT GGGAACCTCT GCACCGAGGA GCAGAGTCCT 
CCGCCGAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTCACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAC 
GAAGAAAAGC CACATACGCA CACAGACAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAACTTACC AGCTTGAGCG GCATCGAATA 
GAGGTGGCTC TAGAACGCAA GTCCGATAGT GATATGTGGG TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CATCTAGCCA AGAGCACCTG AACCATTCCT CTAAGCCGGT CACCCCTGCT 
TCCACGCTGA CCAAAAGCGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC CCTCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCC 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATC TGCCTCTCCC ACCACCTCCT 
TCTGCCAACC AGATAGGACT GCCGTCTGCG CAGGTGGCTG CTGCAGAACG GAAAAAGAGG 
GAAGAGCATC AGCGCTGGTA TGAGAAGGAA AAGGCCCGCC TGGAAGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCCT AAACCCTGCT 
CCGTTTTCTC CCCTCACCAC ACAACAGATG AAGCCCGAAA AGCCTACCAC CCTCCAGAGG 
CCCCAAGAAA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC AGTTAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCTGACCCGT GGAAGCGCGA CGCTAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCAGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAAT TCCAGAAGCG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA CGACGAGGAG GAGGAGGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGA 
CAGCAGCAGT TAGAAGAGAT GCGTAAACGC GAAGCAGAAG ACCGAGCAAG ACAAGAGGAA 
GAGCGCCATC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCATGA CGAGGCGGCA 
CGCAGGTTGT TGGAGCCCGA GGCGCCCGGA CCGAACTCTT ACCCAGGATC TACGGGGGCA 
GCTGTTGGAA CCCATGAGGC GTATCGGGAT GCAAGAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATGTCC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAAGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971554.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827043.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

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CloneID	OCb53679
Clone ID Related Accession (Same CDS sequence)	XM_017971554.1
Accession Version	XM_017971554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTCT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATTCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACTGACC CAGATTCAGG GCTGTGCTCA 
CATTACTGGG GCGCGATCAT CCGTCAGCAG CTGGGCCATA TTGAAGCATG GGCTGAGAAG 
CAGGGGCTGG AGCTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TTCAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGGCTGTGG CTGAAAACAC TGCTGATGAG 
CTGGCCCGCA GCGATGGAAG GGAGGTGCAG TTGGAGGAGG ATCCTGACCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCCCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCGGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC CGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGGCT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTC CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAG CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGATAGA CTGATGAAAA ACAGAGCTGA TCACCGCTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAGGTGCATA TGCCTCTGGA 
ACAGCAGCGA AGATAACATC TGTCTCTACT GGGAACCTCT GCACCGAGGA GCAGAGTCCT 
CCGCCGAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTCACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAC 
GAAGAAAAGC CACATACGCA CACAGACAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAACTTACC AGCTTGAGCG GCATCGAATA 
GAGGTGGCTC TAGAACGCAA GTCCGATAGT GATATGTGGG TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CATCTAGCCA AGAGCACCTG AACCATTCCT CTAAGCCGGT CACCCCTGCT 
TCCACGCTGA CCAAAAGCGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC CCTCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCC 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATC TGCCTCTCCC ACCACCTCCT 
TCTGCCAACC AGATAGGACT GCCGTCTGCG CAGGTGGCTG CTGCAGAACG GAAAAAGAGG 
GAAGAGCATC AGCGCTGGTA TGAGAAGGAA AAGGCCCGCC TGGAAGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCCT AAACCCTGCT 
CCGTTTTCTC CCCTCACCAC ACAACAGATG AAGCCCGAAA AGCCTACCAC CCTCCAGAGG 
CCCCAAGAAA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC AGTTAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCTGACCCGT GGAAGCGCGA CGCTAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCAGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAAT TCCAGAAGCG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA CGACGAGGAG GAGGAGGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGACAGC AGCAGTTAGA AGAGATGCGT 
AAACGCGAAG CAGAAGACCG AGCAAGACAA GAGGAAGAGC GCCATCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGGAC CGAACTCTTA CCCAGGATCT ACGGGGGCAG 
CTGTTGGAAC CCATGAGGCG TATCGGGATG CAAGAGAGAA GCGCTCTAAA AGCCAAGATG 
CAGATGTCCC TGGAAGTTCT GGGGCCCCTG AAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827043.1
CDS	112..5250
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKDRTLTQDLRGQLLEPMRRIGMQERSALKAKMQMSLEVLGPLKT

Target ORF information:

RefSeq Version	XM_017971554.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971554.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAATTAAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTGCTTAAG AATGAGAATG ATGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGAG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC GTTGCGACAG 
GCATCTGATA AAGATGATAG GCCTTTCCAA GGGGAGGACG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCTGAAACC AGCTTCACAC GAACAATTTC TAATCCTGAG 
GTGGTCATGA AACGGCGGAG GCAACAGAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCAGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCA 
GAAGCTTTAG AGAAGTATGG CCTGGAAAAA GAAAACCCGA AGGATTACTG CATAGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAAG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACAAA GAAACACTTG 
GAAGGGAAGC CACCCAAAGG AAAGGAGAAA GCCGACGGGT CTGGCTATGG CTCCACCCTT 
CCCCCTGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA TGAAGATGGT TCTGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAAT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTATTTGGCC CAGGAATTCA GCCTCATCAC TGTGACCTCA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT AGACGCAGAA ACCTATGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGTTCTTGCA AAAAGATCTG TGGATGGAGG CCTCATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT AGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCATCCG CCTCCAGCAC AGCCGAGCGG GGGATGGTGA AGCCAATGAT CAGAGTAGAG 
CAGCAACCAG ATTATCGCAG GCAAGAAAGC AGAACCCAGG ATGCTTCTGG GCCTGACCTG 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTCTT ATCTGCGATT 
ATAAACTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC AACAGAGCGC 
ACGCACAAAG TCATTGCAGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTCT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGACGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATTCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACTGACC CAGATTCAGG GCTGTGCTCA 
CATTACTGGG GCGCGATCAT CCGTCAGCAG CTGGGCCATA TTGAAGCATG GGCTGAGAAG 
CAGGGGCTGG AGCTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TTCAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGGCTGTGG CTGAAAACAC TGCTGATGAG 
CTGGCCCGCA GCGATGGAAG GGAGGTGCAG TTGGAGGAGG ATCCTGACCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCCCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCGGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTCAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC CGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGGCT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTC CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAG CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGATAGA CTGATGAAAA ACAGAGCTGA TCACCGCTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAGGTGCATA TGCCTCTGGA 
ACAGCAGCGA AGATAACATC TGTCTCTACT GGGAACCTCT GCACCGAGGA GCAGAGTCCT 
CCGCCGAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTCACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAC 
GAAGAAAAGC CACATACGCA CACAGACAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAACTTACC AGCTTGAGCG GCATCGAATA 
GAGGTGGCTC TAGAACGCAA GTCCGATAGT GATATGTGGG TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CATCTAGCCA AGAGCACCTG AACCATTCCT CTAAGCCGGT CACCCCTGCT 
TCCACGCTGA CCAAAAGCGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCG TCTCCCAGCC CCTCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCC 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATC TGCCTCTCCC ACCACCTCCT 
TCTGCCAACC AGATAGGACT GCCGTCTGCG CAGGTGGCTG CTGCAGAACG GAAAAAGAGG 
GAAGAGCATC AGCGCTGGTA TGAGAAGGAA AAGGCCCGCC TGGAAGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCCT AAACCCTGCT 
CCGTTTTCTC CCCTCACCAC ACAACAGATG AAGCCCGAAA AGCCTACCAC CCTCCAGAGG 
CCCCAAGAAA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC AGTTAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCTGACCCGT GGAAGCGCGA CGCTAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCAGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAAT TCCAGAAGCG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA CGACGAGGAG GAGGAGGATG ATGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGACAGC AGCAGTTAGA AGAGATGCGT 
AAACGCGAAG CAGAAGACCG AGCAAGACAA GAGGAAGAGC GCCATCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGGAC CGAACTCTTA CCCAGGATCT ACGGGGGCAG 
CTGTTGGAAC CCATGAGGCG TATCGGGATG CAAGAGAGAA GCGCTCTAAA AGCCAAGATG 
CAGATGTCCC TGGAAGTTCT GGGGCCCCTG AAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971555.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827044.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

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CloneID	OCb53680
Clone ID Related Accession (Same CDS sequence)	XM_017971555.1
Accession Version	XM_017971555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTCTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GACGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TTCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CTGACCCAGA TTCAGGGCTG TGCTCACATT ACTGGGGCGC GATCATCCGT 
CAGCAGCTGG GCCATATTGA AGCATGGGCT GAGAAGCAGG GGCTGGAGCT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTTCA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGG CTGTGGCTGA AAACACTGCT GATGAGCTGG CCCGCAGCGA TGGAAGGGAG 
GTGCAGTTGG AGGAGGATCC TGACCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCCCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CGGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTC 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCCGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGGCTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTCCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAGCTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GATAGACTGA TGAAAAACAG AGCTGATCAC CGCTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAGG TGCATATGCC TCTGGAACAG CAGCGAAGAT AACATCTGTC 
TCTACTGGGA ACCTCTGCAC CGAGGAGCAG AGTCCTCCGC CGAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTCACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTACGAAG AAAAGCCACA TACGCACACA 
GACAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAA CTTACCAGCT TGAGCGGCAT CGAATAGAGG TGGCTCTAGA ACGCAAGTCC 
GATAGTGATA TGTGGGTAAA TCAGAGCTCC TCACTGGACT CCAGTACATC TAGCCAAGAG 
CACCTGAACC ATTCCTCTAA GCCGGTCACC CCTGCTTCCA CGCTGACCAA AAGCGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCCCTC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCCGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATCTGCC TCTCCCACCA CCTCCTTCTG CCAACCAGAT AGGACTGCCG 
TCTGCGCAGG TGGCTGCTGC AGAACGGAAA AAGAGGGAAG AGCATCAGCG CTGGTATGAG 
AAGGAAAAGG CCCGCCTGGA AGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCCTAAAC CCTGCTCCGT TTTCTCCCCT CACCACACAA 
CAGATGAAGC CCGAAAAGCC TACCACCCTC CAGAGGCCCC AAGAAACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACAGTT 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCTG ACCCGTGGAA GCGCGACGCT 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC AGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAATTCCA GAAGCGACTC CAGGAGTCGA AGCAGAAGGA CGAAGACGAC 
GAGGAGGAGG AGGATGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGACGAG 
GAGCGGAGGC GACAGCAGCA GTTAGAAGAG ATGCGTAAAC GCGAAGCAGA AGACCGAGCA 
AGACAAGAGG AAGAGCGCCA TCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA 
AAGGTTATGG TCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827044.1
CDS	112..5109
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKVMVL

Target ORF information:

RefSeq Version	XM_017971555.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971555.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTCTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GACGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TTCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CTGACCCAGA TTCAGGGCTG TGCTCACATT ACTGGGGCGC GATCATCCGT 
CAGCAGCTGG GCCATATTGA AGCATGGGCT GAGAAGCAGG GGCTGGAGCT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTTCA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGG CTGTGGCTGA AAACACTGCT GATGAGCTGG CCCGCAGCGA TGGAAGGGAG 
GTGCAGTTGG AGGAGGATCC TGACCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCCCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CGGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTC 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCCGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGGCTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTCCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAGCTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GATAGACTGA TGAAAAACAG AGCTGATCAC CGCTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAGG TGCATATGCC TCTGGAACAG CAGCGAAGAT AACATCTGTC 
TCTACTGGGA ACCTCTGCAC CGAGGAGCAG AGTCCTCCGC CGAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTCACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTACGAAG AAAAGCCACA TACGCACACA 
GACAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAA CTTACCAGCT TGAGCGGCAT CGAATAGAGG TGGCTCTAGA ACGCAAGTCC 
GATAGTGATA TGTGGGTAAA TCAGAGCTCC TCACTGGACT CCAGTACATC TAGCCAAGAG 
CACCTGAACC ATTCCTCTAA GCCGGTCACC CCTGCTTCCA CGCTGACCAA AAGCGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCCCTC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCCGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATCTGCC TCTCCCACCA CCTCCTTCTG CCAACCAGAT AGGACTGCCG 
TCTGCGCAGG TGGCTGCTGC AGAACGGAAA AAGAGGGAAG AGCATCAGCG CTGGTATGAG 
AAGGAAAAGG CCCGCCTGGA AGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCCTAAAC CCTGCTCCGT TTTCTCCCCT CACCACACAA 
CAGATGAAGC CCGAAAAGCC TACCACCCTC CAGAGGCCCC AAGAAACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACAGTT 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCTG ACCCGTGGAA GCGCGACGCT 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC AGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAATTCCA GAAGCGACTC CAGGAGTCGA AGCAGAAGGA CGAAGACGAC 
GAGGAGGAGG AGGATGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGACGAG 
GAGCGGAGGC GACAGCAGCA GTTAGAAGAG ATGCGTAAAC GCGAAGCAGA AGACCGAGCA 
AGACAAGAGG AAGAGCGCCA TCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA 
AAGGTTATGG TCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971556.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827045.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

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CloneID	OCb53681
Clone ID Related Accession (Same CDS sequence)	XM_017971556.1
Accession Version	XM_017971556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTCTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GACGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TTCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CTGACCCAGA TTCAGGGCTG TGCTCACATT ACTGGGGCGC GATCATCCGT 
CAGCAGCTGG GCCATATTGA AGCATGGGCT GAGAAGCAGG GGCTGGAGCT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTTCA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGG CTGTGGCTGA AAACACTGCT GATGAGCTGG CCCGCAGCGA TGGAAGGGAG 
GTGCAGTTGG AGGAGGATCC TGACCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCCCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CGGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTC 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCCGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGGCTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTCCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAGCTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GATAGACTGA TGAAAAACAG AGCTGATCAC CGCTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAGG TGCATATGCC TCTGGAACAG CAGCGAAGAT AACATCTGTC 
TCTACTGGGA ACCTCTGCAC CGAGGAGCAG AGTCCTCCGC CGAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTCACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTACGAAG AAAAGCCACA TACGCACACA 
GACAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAA CTTACCAGCT TGAGCGGCAT CGAATAGAGG TGGCTCTAGA ACGCAAGTCC 
GATAGTGATA TGTGGGTAAA TCAGAGCTCC TCACTGGACT CCAGTACATC TAGCCAAGAG 
CACCTGAACC ATTCCTCTAA GCCGGTCACC CCTGCTTCCA CGCTGACCAA AAGCGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCCCTC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCCGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATCTGCC TCTCCCACCA CCTCCTTCTG CCAACCAGAT AGGACTGCCG 
TCTGCGCAGG TGGCTGCTGC AGAACGGAAA AAGAGGGAAG AGCATCAGCG CTGGTATGAG 
AAGGAAAAGG CCCGCCTGGA AGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCCTAAAC CCTGCTCCGT TTTCTCCCCT CACCACACAA 
CAGATGAAGC CCGAAAAGCC TACCACCCTC CAGAGGCCCC AAGAAACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACAGTT 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCTG ACCCGTGGAA GCGCGACGCT 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC AGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAATTCCA GAAGCGACTC CAGGAGTCGA AGCAGAAGGA CGAAGACGAC 
GAGGAGGAGG AGGATGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGA CAGCAGCAGT TAGAAGAGAT GCGTAAACGC 
GAAGCAGAAG ACCGAGCAAG ACAAGAGGAA GAGCGCCATC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GGTTATGGTC CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827045.1
CDS	112..5067
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKVMVL

Target ORF information:

RefSeq Version	XM_017971556.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971556.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGAGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCGTT GCGACAGGCA 
TCTGATAAAG ATGATAGGCC TTTCCAAGGG GAGGACGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC TGAAACCAGC TTCACACGAA CAATTTCTAA TCCTGAGGTG 
GTCATGAAAC GGCGGAGGCA ACAGAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CAGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCAGAA 
GCTTTAGAGA AGTATGGCCT GGAAAAAGAA AACCCGAAGG ATTACTGCAT AGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAAGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACAAAGAA ACACTTGGAA 
GGGAAGCCAC CCAAAGGAAA GGAGAAAGCC GACGGGTCTG GCTATGGCTC CACCCTTCCC 
CCTGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTATGA AGATGGTTCT GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAATTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTA 
TTTGGCCCAG GAATTCAGCC TCATCACTGT GACCTCACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATAGA CGCAGAAACC TATGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGT TCTTGCAAAA AGATCTGTGG ATGGAGGCCT CATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCATCCGCCT CCAGCACAGC CGAGCGGGGG ATGGTGAAGC CAATGATCAG AGTAGAGCAG 
CAACCAGATT ATCGCAGGCA AGAAAGCAGA ACCCAGGATG CTTCTGGGCC TGACCTGATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTCTTATC TGCGATTATA 
AACTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCAAC AGAGCGCACG 
CACAAAGTCA TTGCAGTTGT CAACAAGATG GTGAGCATGA TGGAAGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTCTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GACGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TTCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CTGACCCAGA TTCAGGGCTG TGCTCACATT ACTGGGGCGC GATCATCCGT 
CAGCAGCTGG GCCATATTGA AGCATGGGCT GAGAAGCAGG GGCTGGAGCT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTTCA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGG CTGTGGCTGA AAACACTGCT GATGAGCTGG CCCGCAGCGA TGGAAGGGAG 
GTGCAGTTGG AGGAGGATCC TGACCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCCCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CGGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTC 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCCGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGGCTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTCCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAGCTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GATAGACTGA TGAAAAACAG AGCTGATCAC CGCTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAGG TGCATATGCC TCTGGAACAG CAGCGAAGAT AACATCTGTC 
TCTACTGGGA ACCTCTGCAC CGAGGAGCAG AGTCCTCCGC CGAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTCACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTACGAAG AAAAGCCACA TACGCACACA 
GACAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAA CTTACCAGCT TGAGCGGCAT CGAATAGAGG TGGCTCTAGA ACGCAAGTCC 
GATAGTGATA TGTGGGTAAA TCAGAGCTCC TCACTGGACT CCAGTACATC TAGCCAAGAG 
CACCTGAACC ATTCCTCTAA GCCGGTCACC CCTGCTTCCA CGCTGACCAA AAGCGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCGTCTC CCAGCCCCTC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCCGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATCTGCC TCTCCCACCA CCTCCTTCTG CCAACCAGAT AGGACTGCCG 
TCTGCGCAGG TGGCTGCTGC AGAACGGAAA AAGAGGGAAG AGCATCAGCG CTGGTATGAG 
AAGGAAAAGG CCCGCCTGGA AGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCCTAAAC CCTGCTCCGT TTTCTCCCCT CACCACACAA 
CAGATGAAGC CCGAAAAGCC TACCACCCTC CAGAGGCCCC AAGAAACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACAGTT 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCTG ACCCGTGGAA GCGCGACGCT 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC AGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAATTCCA GAAGCGACTC CAGGAGTCGA AGCAGAAGGA CGAAGACGAC 
GAGGAGGAGG AGGATGATGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGA CAGCAGCAGT TAGAAGAGAT GCGTAAACGC 
GAAGCAGAAG ACCGAGCAAG ACAAGAGGAA GAGCGCCATC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GGTTATGGTC CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_017971557.1 ) cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset) -> XP_017827046.1 Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

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CloneID	OCb53682
Clone ID Related Accession (Same CDS sequence)	XM_017971557.1
Accession Version	XM_017971557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013899.1) and transcript sequence (GAMP01013808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callithrix jacchus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 216 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGAGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCGTTGCG ACAGGCATCT 
GATAAAGATG ATAGGCCTTT CCAAGGGGAG GACGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCTGA AACCAGCTTC ACACGAACAA TTTCTAATCC TGAGGTGGTC 
ATGAAACGGC GGAGGCAACA GAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCAG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCAGAAGCT 
TTAGAGAAGT ATGGCCTGGA AAAAGAAAAC CCGAAGGATT ACTGCATAGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CAAAGAAACA CTTGGAAGGG 
AAGCCACCCA AAGGAAAGGA GAAAGCCGAC GGGTCTGGCT ATGGCTCCAC CCTTCCCCCT 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTTCAATT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TATTTGGCCC AGGAATTCAG CCTCATCACT GTGACCTCAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATA GACGCAGAAA CCTATGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GTTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTCATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCATCCGC CTCCAGCACA GCCGAGCGGG GGATGGTGAA GCCAATGATC 
AGAGTAGAGC AGCAACCAGA TTATCGCAGG CAAGAAAGCA GAACCCAGGA TGCTTCTGGG 
CCTGACCTGA TACTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTCTTA 
TCTGCGATTA TAAACTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCA 
ACAGAGCGCA CGCACAAAGT CATTGCAGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GAATCACATT GGATGCCCAA 
GATGTTCTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGACGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATTCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACTGACCCA GATTCAGGGC TGTGCTCACA TTACTGGGGC 
GCGATCATCC GTCAGCAGCT GGGCCATATT GAAGCATGGG CTGAGAAGCA GGGGCTGGAG 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTT 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GGCTGTGGCT GAAAACACTG CTGATGAGCT GGCCCGCAGC 
GATGGAAGGG AGGTGCAGTT GGAGGAGGAT CCTGACCTGC AGCTGCCTTT TCTTTTGCCG 
GAAGACGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCCCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCGGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TCAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCCG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGGCTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTCCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAGCT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGATAGACT GATGAAAAAC AGAGCTGATC ACCGCTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA GGTGCATATG CCTCTGGAAC AGCAGCGAAG 
ATAACATCTG TCTCTACTGG GAACCTCTGC ACCGAGGAGC AGAGTCCTCC GCCGAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTCACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTACGA AGAAAAGCCA 
CATACGCACA CAGACAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AACTTACCAG CTTGAGCGGC ATCGAATAGA GGTGGCTCTA 
GAACGCAAGT CCGATAGTGA TATGTGGGTA AATCAGAGCT CCTCACTGGA CTCCAGTACA 
TCTAGCCAAG AGCACCTGAA CCATTCCTCT AAGCCGGTCA CCCCTGCTTC CACGCTGACC 
AAAAGCGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC 
TCCCAGCCCC TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCCGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATCTG CCTCTCCCAC CACCTCCTTC TGCCAACCAG 
ATAGGACTGC CGTCTGCGCA GGTGGCTGCT GCAGAACGGA AAAAGAGGGA AGAGCATCAG 
CGCTGGTATG AGAAGGAAAA GGCCCGCCTG GAAGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCCTAA ACCCTGCTCC GTTTTCTCCC 
CTCACCACAC AACAGATGAA GCCCGAAAAG CCTACCACCC TCCAGAGGCC CCAAGAAACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACAG TTAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC TGACCCGTGG 
AAGCGCGACG CTAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCAGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAATTC CAGAAGCGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGACG ACGAGGAGGA GGAGGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGACA GCAGCAGTTA 
GAAGAGATGC GTAAACGCGA AGCAGAAGAC CGAGCAAGAC AAGAGGAAGA GCGCCATCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCATGACG AGGCGGCACG CAGGTTGTTG 
GAGCCCGAGG CGCCCGGACC GAACTCTTAC CCAGGATCTA CGGGGGCAGC TGTTGGAACC 
CATGAGGCGT ATCGGGATGC AAGAGAGAAG CGCTCTAAAA GCCAAGATGC AGATGTCCCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAAGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017827046.1
CDS	112..5346
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKPPKGKEKADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSIDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHVLAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGAYASGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHTHTDSNHSSIAIQRVTRSQEELREDKTYQLERHRIEVALERKSDSDMWVNQSSSLDSSTSSQEHLNHSSKPVTPASTLTKSGPGRWKTPAAIPATPVAVSQPLRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTTQQMKPEKPTTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERHRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGTHEAYRDAREKRSKSQDADVPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_017971557.1
Organism	Callithrix jacchus(white-tufted-ear marmoset)
Definition	Callithrix jacchus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017971557.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAG GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CACTCGCAGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC CTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AATTAAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT GCTTAAGAAT GAGAATGATG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGAGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCGTTGCG ACAGGCATCT 
GATAAAGATG ATAGGCCTTT CCAAGGGGAG GACGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCTGA AACCAGCTTC ACACGAACAA TTTCTAATCC TGAGGTGGTC 
ATGAAACGGC GGAGGCAACA GAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCAG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCAGAAGCT 
TTAGAGAAGT ATGGCCTGGA AAAAGAAAAC CCGAAGGATT ACTGCATAGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAAGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CAAAGAAACA CTTGGAAGGG 
AAGCCACCCA AAGGAAAGGA GAAAGCCGAC GGGTCTGGCT ATGGCTCCAC CCTTCCCCCT 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTTCAATT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TATTTGGCCC AGGAATTCAG CCTCATCACT GTGACCTCAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATA GACGCAGAAA CCTATGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GTTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTCATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCATCCGC CTCCAGCACA GCCGAGCGGG GGATGGTGAA GCCAATGATC 
AGAGTAGAGC AGCAACCAGA TTATCGCAGG CAAGAAAGCA GAACCCAGGA TGCTTCTGGG 
CCTGACCTGA TACTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTCTTA 
TCTGCGATTA TAAACTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCA 
ACAGAGCGCA CGCACAAAGT CATTGCAGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GAATCACATT GGATGCCCAA 
GATGTTCTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGACGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATTCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACTGACCCA GATTCAGGGC TGTGCTCACA TTACTGGGGC 
GCGATCATCC GTCAGCAGCT GGGCCATATT GAAGCATGGG CTGAGAAGCA GGGGCTGGAG 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTT 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GGCTGTGGCT GAAAACACTG CTGATGAGCT GGCCCGCAGC 
GATGGAAGGG AGGTGCAGTT GGAGGAGGAT CCTGACCTGC AGCTGCCTTT TCTTTTGCCG 
GAAGACGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCCCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCGGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TCAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCCG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGGCTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTCCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAGCT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGATAGACT GATGAAAAAC AGAGCTGATC ACCGCTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA GGTGCATATG CCTCTGGAAC AGCAGCGAAG 
ATAACATCTG TCTCTACTGG GAACCTCTGC ACCGAGGAGC AGAGTCCTCC GCCGAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTCACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTACGA AGAAAAGCCA 
CATACGCACA CAGACAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AACTTACCAG CTTGAGCGGC ATCGAATAGA GGTGGCTCTA 
GAACGCAAGT CCGATAGTGA TATGTGGGTA AATCAGAGCT CCTCACTGGA CTCCAGTACA 
TCTAGCCAAG AGCACCTGAA CCATTCCTCT AAGCCGGTCA CCCCTGCTTC CACGCTGACC 
AAAAGCGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCGTC 
TCCCAGCCCC TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCCGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATCTG CCTCTCCCAC CACCTCCTTC TGCCAACCAG 
ATAGGACTGC CGTCTGCGCA GGTGGCTGCT GCAGAACGGA AAAAGAGGGA AGAGCATCAG 
CGCTGGTATG AGAAGGAAAA GGCCCGCCTG GAAGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCCTAA ACCCTGCTCC GTTTTCTCCC 
CTCACCACAC AACAGATGAA GCCCGAAAAG CCTACCACCC TCCAGAGGCC CCAAGAAACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACAG TTAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC TGACCCGTGG 
AAGCGCGACG CTAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCAGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAATTC CAGAAGCGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGACG ACGAGGAGGA GGAGGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGACA GCAGCAGTTA 
GAAGAGATGC GTAAACGCGA AGCAGAAGAC CGAGCAAGAC AAGAGGAAGA GCGCCATCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCATGACG AGGCGGCACG CAGGTTGTTG 
GAGCCCGAGG CGCCCGGACC GAACTCTTAC CCAGGATCTA CGGGGGCAGC TGTTGGAACC 
CATGAGGCGT ATCGGGATGC AAGAGAGAAG CGCTCTAAAA GCCAAGATGC AGATGTCCCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAAGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AFDN
Entrez Gene ID	100398334
Full Name	afadin, adherens junction formation factor
Gene Type	protein-coding
Organism	Callithrix jacchus(white-tufted-ear marmoset)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AFDN cDNA ORF clone, Callithrix jacchus(white-tufted-ear marmoset)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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