AGRN gene cDNA ORF clone, Canis lupus familiaris(Dog)

mRNA	Protein	Name
XM_005620360.3	XP_005620417.1	agrin isoform X2
XM_005620363.2	XP_005620420.1	agrin isoform X5
XM_005620364.1	XP_005620421.1	agrin isoform X6
XM_005620359.2	XP_005620416.1	agrin isoform X1
XM_005620360.2	XP_005620417.1	agrin isoform X2
XM_005620363.1	XP_005620420.1	agrin isoform X5
XM_005620361.3	XP_005620418.1	agrin isoform X3
XM_536713.6	XP_536713.3	agrin isoform X7
XM_005620366.2	XP_005620423.1	agrin isoform X9
XM_005620364.2	XP_005620421.1	agrin isoform X6
XM_005620366.3	XP_005620423.1	agrin isoform X9
XM_005620362.2	XP_005620419.1	agrin isoform X4
XM_005620365.2	XP_005620422.1	agrin isoform X8
XM_005620359.3	XP_005620416.1	agrin isoform X1
XM_005620362.3	XP_005620419.1	agrin isoform X4
XM_005620365.3	XP_005620422.1	agrin isoform X8
XM_005620361.2	XP_005620418.1	agrin isoform X3
XM_536713.5	XP_536713.3	agrin isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe06700	XM_005620365.3 Latest version!	Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe06701	XM_005620366.3 Latest version!	Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe06693	XM_005620359.3 Latest version!	Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06694	XM_005620360.3 Latest version!	Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06695	XM_005620361.3 Latest version!	Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06696	XM_005620362.3 Latest version!	Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06697	XM_005620363.2 Latest version!	Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06698	XM_005620364.2 Latest version!	Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06699	XM_536713.6 Latest version!	Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe06694	XM_005620360.2	Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06697	XM_005620363.1	Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06698	XM_005620364.1	Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06701	XM_005620366.2	Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe06693	XM_005620359.2	Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06696	XM_005620362.2	Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06700	XM_005620365.2	Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe06695	XM_005620361.2	Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe06699	XM_536713.5	Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

AGRN ( XM_005620365.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620422.1 Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe06700
Clone ID Related Accession (Same CDS sequence)	XM_005620365.2 , XM_005620365.3
Accession Version	XM_005620365.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agrin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC 
TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC 
CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC 
AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG 
GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG 
CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT 
GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC 
CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT 
GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG 
CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC 
AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT 
CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT 
AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT 
GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG 
TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG 
GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT 
GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG 
ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG 
TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC 
ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA 
GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA 
CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC 
CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG 
CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT 
GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT 
GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA 
GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC 
TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC 
CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG 
TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT 
CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC 
TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA 
TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC 
CTTGGCCCGT GCCAGGACTC TGTTCTCCTT GCAGAGACTG TTGCTCCTGG GGCCCGCCCA 
ACATCTGTAC CCAGGGCCAC CTCAGGGCTC AGCACGAGCA AGGCGCTGCC ACCCGCCCCC 
AGCACCCTCC CCCTGGCTCC CAGCAGCACC CCTCATAGCC GGCCCACGCC TCGACCCTCA 
CCAGGATCTC GGACCACTGC CAGCATTCCC AGGACTGTCA CGAGGCCTGT GCTGACCATG 
CCCCCCACAG CCCCCGCCAC GGCAGCCAAC CTCGCCACAT CTGCTTTTGG CGAGTCTGGC 
AGTGCTGACG GGAGCGGCGA TGAGGGGCTG AGTGGTGACC TGGAGGCCAG TGGGACTGGC 
TCAGGGGGAC TCGAGCCCCC TGAAGGTGGC AGTGCTGTGA CCCCTGAGCC ACCCATGGAG 
AGGGCTTCTT GCTACAACTC GCCTTTGGGC TGCTGCTCGG ATGGCAAGAC GCCCTCGCTG 
GACGCAGAGG GTTCCAACTG TCCTGCCACC AAGGCGTTCC AGGGTGTGTT GGAGCTTGAG 
GGCGTGGAGG GGCAAGAGCT GTTCTACACG CCGGAGATGG CTGACCCCAA GTCAGAGCTT 
TTTGGGGAGA CTGCTAGGAG CATCGAGAGC ACCCTGGATG ACCTCTTCCG GAACTCAGAG 
GTTAAGAAGG ACTTCCGAAG CGTCCGGCTG AGGGACTTGG GGCCTGGCAG CTCAGTCCGA 
GCCATTGTGG ATGTGCACTT TGACCCTGCC ACAACCTTTA GGGCGCCTGA TGTGGGCCAA 
GCCCTGCTCC GGCAGATACA GGCATCCCGG CGCCGGTCCC TGGGCGTACG GCGGCCACTG 
CAGGAGCACG TGCGCTTTAT GGACTTTGAC TGGTTTCCTG CGTTCTTCAC GGGAGCCACC 
ACAGGAGCAA CACCCGCCGC AGCCACGGCC AGAGCCACCA CGGTGGCCCA CCTGCCACCT 
GCTGGGACCC AGTGGGCCCT CTACCCCAGT CACACAAGCC GGCCTGTGGG CAGGACCACA 
GCTCCACCCA CCACACGCCA GCCTCCCACC ACTGCTCCCC GTCGCGTGCC CGGACGCCGG 
CCCTTGCCCC CAGGCACCCA GCAGCCCCCG AAGCCCTGCG ACTCCCAGCC CTGCCTCCAT 
GGGGGAACCT GCCAGGACCA GGACTCGGGT GGAGGCTTCA CCTGCAGCTG CCCAGTGGGC 
AGGAGGGGTG CCGTCTGTGA GAAGGCCCTG TACCTCTCGG TGCCAGCTTT CAATGGCCGC 
TCCTTCCTGG CCTTCCCCAC CCTCCGTGCC TACCACACGC TGCGCCTAGC ACTTGAGTTC 
CGGGCACTAG AGCCACAGGG CCTGCTGCTC TATAATGGCA ATGCCCAGGG CAAGGACTTC 
CTGGCATTGA CGCTGCTGGG GGGCCGTGTG CAGCTCAGGT TTGACACAGG TTCCGGGCCT 
GCAGTACTGA CCAGCTCAGT GCCTATAGAG CCTGGCCGCT GGCATCGCTT GGAGCTGTCT 
CGGCACTGGC GCCGGGGAAC ACTTTCAGTG GATGGTGAGC CCCTGGTGCT GGGCCACAGC 
CCCAGCGGTA CAGATGGTCT CAACCTGGAC ACAGACCTTT TTGTGGGCGG CATCCCTGAG 
GATCAGGCTT CTGTGGTACT CGAAAGGACG TCCATCAGCG TTGGCCTCAA GGGCTGCATC 
CGCCTGCTGG ATGTCAATAA TCAGCGTTTA GAGCTCAGCA GCTGGCCAGG GGCTGCAGCC 
CGAAGCTCCG GGGTGGGCGA GTGTGGAGAC CACCCCTGCC TGCCCAACCC CTGCCTCGAC 
GGGGCTCCGT GCCAGGCCCT GGAGGCTGGC ATGTTCCACT GTCAGTGCCC ACCTGGCCGC 
TTTGGTCCAA CCTGTAGTGG TGACAAGAAC CCCTGTGAGC CGAACCCCTG CCATGGGGCA 
GCCCCCTGCC GTGTGCTGCC TGATGGGGAG GCCAAGTGTG AATGCCCCCG AGGACGAGGG 
GGTGCCATCT GCCAGACAGT CTCGGAGCGG GATTCTTCCC AGCCCTTCCT GGCTGACTTC 
CATGGCTTCT CCTACCTGGA GCTGAAAGGG TTGCATACCT TTGAGCGGGA CCTGGGGGAG 
AAGCTGGCAC TGGAGGTGGT GTTCCTGGCC CGTGGGCCCA GTGGTTTGCT CCTCTACAAT 
GGGCAGAAAA CAGACGGCAA GGGGGACTTT GTGTCACTGG CGTTGCATGA CCGCTTCTTG 
GAATTCCGCT ATGACCTGGG CAAGGGAGCA GCGGTCATCA GGAGCAAGGA GCCGGTGGCC 
CTGGACACCT GGACCAGGGT CTCACTAGAG CGCAATGGCC GCAAGGGTGC CATGCGTGTC 
AGTGATGGGC CTCGCGTGCT GGGAGAGTCC CCGAAATCCC GCAAGGTGCC ACACACTGTC 
CTCAACCTGA AGGAGCCACT CTATGTTGGA GGGGCCCCTG ACTTCAGCAA ACTGGCCCGG 
GCAGCTGCGG TATTCTCTGG CTTCGATGGC GCCATCCAAC TGGTCTCCTT GAAAGGCCAC 
CAGCTGCTGT CCCAGGAGCA CGTGGTGCGG TCAGTGGATG TCTCATCTTT TGCCCACCAC 
CCTTGTACTC AGGCCTCGGG CCACCCATGC CTCAATGGGG CCTCCTGCCT CCCTCGAGAG 
GCCTCCTATG AGTGCCTGTG CCCCGGGGGC TTCTCAGGGC TTCACTGTGA GAAGGGGCTG 
ATTGAGAAGT CAGCAGGGGA TGTGGACGCC CTGGCCTTTG ACGGACAGAC CTACGTCGAG 
TACCTCAATG CCGTGACTGA GAGCGAACTG ACCAATGAGA TCCCAGCTCC TGATGCTCCG 
GATTCCGGGG CCCTTCCCAG TGAGAAGGCC CTGCAGAGCA ACCACTTTGA GCTGAGCCTG 
CGCACGGAAG CCACTCAGGG GCTGGTGCTG TGGAGCGGCA AGGCCACGGA GCGGGCCGAC 
TATGTCGCAC TGGCCATCGT GGATGGGCGC CTGCAGCTGA CCTATGACCT GGGCTCCCAG 
CCCGTCGTGC TGCGCTCCAC CGTGCCGGTC AACACCAACC GCTGGCTACG AGTCAGGGCA 
CATAGGGAAC AGAGGGAAGG TTCCCTTCAG GTGGGCAATG AGGCTCCCGT GACTGGCTCT 
TCCCCGCTGG GTGCCACACA GCTAGACACA GACGGAACTC TATGGCTGGG TGGCCTGGAG 
AAGCTGCCCA TAGGCCAGGC CCTGCCCAAG GCCTATGGCA CGGGCTTTGT GGGCTGCCTG 
CGGGATGTGG TGGTGGGGCA GCGCCCACTG CACCTGCTGG AGGATGCTGT CACCAAGCCA 
GAGCTCCGAC CCTGTGCCGC CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620422.1
CDS	747..6653
Translation	MPPPPLERDPRQQRGVSLLVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620365.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620365.2

ORF Insert Sequence:

ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC 
TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC 
CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC 
AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG 
GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG 
CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT 
GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC 
CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT 
GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG 
CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC 
AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT 
CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT 
AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT 
GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG 
TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG 
GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT 
GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG 
ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG 
TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC 
ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA 
GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA 
CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC 
CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG 
CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT 
GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT 
GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA 
GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC 
TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC 
CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG 
TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT 
CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC 
TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA 
TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC 
CTTGGCCCGT GCCAGGACTC TGTTCTCCTT GCAGAGACTG TTGCTCCTGG GGCCCGCCCA 
ACATCTGTAC CCAGGGCCAC CTCAGGGCTC AGCACGAGCA AGGCGCTGCC ACCCGCCCCC 
AGCACCCTCC CCCTGGCTCC CAGCAGCACC CCTCATAGCC GGCCCACGCC TCGACCCTCA 
CCAGGATCTC GGACCACTGC CAGCATTCCC AGGACTGTCA CGAGGCCTGT GCTGACCATG 
CCCCCCACAG CCCCCGCCAC GGCAGCCAAC CTCGCCACAT CTGCTTTTGG CGAGTCTGGC 
AGTGCTGACG GGAGCGGCGA TGAGGGGCTG AGTGGTGACC TGGAGGCCAG TGGGACTGGC 
TCAGGGGGAC TCGAGCCCCC TGAAGGTGGC AGTGCTGTGA CCCCTGAGCC ACCCATGGAG 
AGGGCTTCTT GCTACAACTC GCCTTTGGGC TGCTGCTCGG ATGGCAAGAC GCCCTCGCTG 
GACGCAGAGG GTTCCAACTG TCCTGCCACC AAGGCGTTCC AGGGTGTGTT GGAGCTTGAG 
GGCGTGGAGG GGCAAGAGCT GTTCTACACG CCGGAGATGG CTGACCCCAA GTCAGAGCTT 
TTTGGGGAGA CTGCTAGGAG CATCGAGAGC ACCCTGGATG ACCTCTTCCG GAACTCAGAG 
GTTAAGAAGG ACTTCCGAAG CGTCCGGCTG AGGGACTTGG GGCCTGGCAG CTCAGTCCGA 
GCCATTGTGG ATGTGCACTT TGACCCTGCC ACAACCTTTA GGGCGCCTGA TGTGGGCCAA 
GCCCTGCTCC GGCAGATACA GGCATCCCGG CGCCGGTCCC TGGGCGTACG GCGGCCACTG 
CAGGAGCACG TGCGCTTTAT GGACTTTGAC TGGTTTCCTG CGTTCTTCAC GGGAGCCACC 
ACAGGAGCAA CACCCGCCGC AGCCACGGCC AGAGCCACCA CGGTGGCCCA CCTGCCACCT 
GCTGGGACCC AGTGGGCCCT CTACCCCAGT CACACAAGCC GGCCTGTGGG CAGGACCACA 
GCTCCACCCA CCACACGCCA GCCTCCCACC ACTGCTCCCC GTCGCGTGCC CGGACGCCGG 
CCCTTGCCCC CAGGCACCCA GCAGCCCCCG AAGCCCTGCG ACTCCCAGCC CTGCCTCCAT 
GGGGGAACCT GCCAGGACCA GGACTCGGGT GGAGGCTTCA CCTGCAGCTG CCCAGTGGGC 
AGGAGGGGTG CCGTCTGTGA GAAGGCCCTG TACCTCTCGG TGCCAGCTTT CAATGGCCGC 
TCCTTCCTGG CCTTCCCCAC CCTCCGTGCC TACCACACGC TGCGCCTAGC ACTTGAGTTC 
CGGGCACTAG AGCCACAGGG CCTGCTGCTC TATAATGGCA ATGCCCAGGG CAAGGACTTC 
CTGGCATTGA CGCTGCTGGG GGGCCGTGTG CAGCTCAGGT TTGACACAGG TTCCGGGCCT 
GCAGTACTGA CCAGCTCAGT GCCTATAGAG CCTGGCCGCT GGCATCGCTT GGAGCTGTCT 
CGGCACTGGC GCCGGGGAAC ACTTTCAGTG GATGGTGAGC CCCTGGTGCT GGGCCACAGC 
CCCAGCGGTA CAGATGGTCT CAACCTGGAC ACAGACCTTT TTGTGGGCGG CATCCCTGAG 
GATCAGGCTT CTGTGGTACT CGAAAGGACG TCCATCAGCG TTGGCCTCAA GGGCTGCATC 
CGCCTGCTGG ATGTCAATAA TCAGCGTTTA GAGCTCAGCA GCTGGCCAGG GGCTGCAGCC 
CGAAGCTCCG GGGTGGGCGA GTGTGGAGAC CACCCCTGCC TGCCCAACCC CTGCCTCGAC 
GGGGCTCCGT GCCAGGCCCT GGAGGCTGGC ATGTTCCACT GTCAGTGCCC ACCTGGCCGC 
TTTGGTCCAA CCTGTAGTGG TGACAAGAAC CCCTGTGAGC CGAACCCCTG CCATGGGGCA 
GCCCCCTGCC GTGTGCTGCC TGATGGGGAG GCCAAGTGTG AATGCCCCCG AGGACGAGGG 
GGTGCCATCT GCCAGACAGT CTCGGAGCGG GATTCTTCCC AGCCCTTCCT GGCTGACTTC 
CATGGCTTCT CCTACCTGGA GCTGAAAGGG TTGCATACCT TTGAGCGGGA CCTGGGGGAG 
AAGCTGGCAC TGGAGGTGGT GTTCCTGGCC CGTGGGCCCA GTGGTTTGCT CCTCTACAAT 
GGGCAGAAAA CAGACGGCAA GGGGGACTTT GTGTCACTGG CGTTGCATGA CCGCTTCTTG 
GAATTCCGCT ATGACCTGGG CAAGGGAGCA GCGGTCATCA GGAGCAAGGA GCCGGTGGCC 
CTGGACACCT GGACCAGGGT CTCACTAGAG CGCAATGGCC GCAAGGGTGC CATGCGTGTC 
AGTGATGGGC CTCGCGTGCT GGGAGAGTCC CCGAAATCCC GCAAGGTGCC ACACACTGTC 
CTCAACCTGA AGGAGCCACT CTATGTTGGA GGGGCCCCTG ACTTCAGCAA ACTGGCCCGG 
GCAGCTGCGG TATTCTCTGG CTTCGATGGC GCCATCCAAC TGGTCTCCTT GAAAGGCCAC 
CAGCTGCTGT CCCAGGAGCA CGTGGTGCGG TCAGTGGATG TCTCATCTTT TGCCCACCAC 
CCTTGTACTC AGGCCTCGGG CCACCCATGC CTCAATGGGG CCTCCTGCCT CCCTCGAGAG 
GCCTCCTATG AGTGCCTGTG CCCCGGGGGC TTCTCAGGGC TTCACTGTGA GAAGGGGCTG 
ATTGAGAAGT CAGCAGGGGA TGTGGACGCC CTGGCCTTTG ACGGACAGAC CTACGTCGAG 
TACCTCAATG CCGTGACTGA GAGCGAACTG ACCAATGAGA TCCCAGCTCC TGATGCTCCG 
GATTCCGGGG CCCTTCCCAG TGAGAAGGCC CTGCAGAGCA ACCACTTTGA GCTGAGCCTG 
CGCACGGAAG CCACTCAGGG GCTGGTGCTG TGGAGCGGCA AGGCCACGGA GCGGGCCGAC 
TATGTCGCAC TGGCCATCGT GGATGGGCGC CTGCAGCTGA CCTATGACCT GGGCTCCCAG 
CCCGTCGTGC TGCGCTCCAC CGTGCCGGTC AACACCAACC GCTGGCTACG AGTCAGGGCA 
CATAGGGAAC AGAGGGAAGG TTCCCTTCAG GTGGGCAATG AGGCTCCCGT GACTGGCTCT 
TCCCCGCTGG GTGCCACACA GCTAGACACA GACGGAACTC TATGGCTGGG TGGCCTGGAG 
AAGCTGCCCA TAGGCCAGGC CCTGCCCAAG GCCTATGGCA CGGGCTTTGT GGGCTGCCTG 
CGGGATGTGG TGGTGGGGCA GCGCCCACTG CACCTGCTGG AGGATGCTGT CACCAAGCCA 
GAGCTCCGAC CCTGTGCCGC CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620365.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620422.1 Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA.

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CloneID	OCe06700
Clone ID Related Accession (Same CDS sequence)	XM_005620365.2 , XM_005620365.3
Accession Version	XM_005620365.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agrin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC 
TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC 
CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC 
AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG 
GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG 
CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT 
GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC 
CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT 
GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG 
CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC 
AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT 
CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT 
AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT 
GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG 
TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG 
GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT 
GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG 
ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG 
TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC 
ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA 
GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA 
CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC 
CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG 
CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT 
GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT 
GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA 
GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC 
TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC 
CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG 
TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT 
CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC 
TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA 
TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC 
CTTGGCCCGT GCCAGGACTC TGTTCTCCTT GCAGAGACTG TTGCTCCTGG GGCCCGCCCA 
ACATCTGTAC CCAGGGCCAC CTCAGGGCTC AGCACGAGCA AGGCGCTGCC ACCCGCCCCC 
AGCACCCTCC CCCTGGCTCC CAGCAGCACC CCTCATAGCC GGCCCACGCC TCGACCCTCA 
CCAGGATCTC GGACCACTGC CAGCATTCCC AGGACTGTCA CGAGGCCTGT GCTGACCATG 
CCCCCCACAG CCCCCGCCAC GGCAGCCAAC CTCGCCACAT CTGCTTTTGG CGAGTCTGGC 
AGTGCTGACG GGAGCGGCGA TGAGGGGCTG AGTGGTGACC TGGAGGCCAG TGGGACTGGC 
TCAGGGGGAC TCGAGCCCCC TGAAGGTGGC AGTGCTGTGA CCCCTGAGCC ACCCATGGAG 
AGGGCTTCTT GCTACAACTC GCCTTTGGGC TGCTGCTCGG ATGGCAAGAC GCCCTCGCTG 
GACGCAGAGG GTTCCAACTG TCCTGCCACC AAGGCGTTCC AGGGTGTGTT GGAGCTTGAG 
GGCGTGGAGG GGCAAGAGCT GTTCTACACG CCGGAGATGG CTGACCCCAA GTCAGAGCTT 
TTTGGGGAGA CTGCTAGGAG CATCGAGAGC ACCCTGGATG ACCTCTTCCG GAACTCAGAG 
GTTAAGAAGG ACTTCCGAAG CGTCCGGCTG AGGGACTTGG GGCCTGGCAG CTCAGTCCGA 
GCCATTGTGG ATGTGCACTT TGACCCTGCC ACAACCTTTA GGGCGCCTGA TGTGGGCCAA 
GCCCTGCTCC GGCAGATACA GGCATCCCGG CGCCGGTCCC TGGGCGTACG GCGGCCACTG 
CAGGAGCACG TGCGCTTTAT GGACTTTGAC TGGTTTCCTG CGTTCTTCAC GGGAGCCACC 
ACAGGAGCAA CACCCGCCGC AGCCACGGCC AGAGCCACCA CGGTGGCCCA CCTGCCACCT 
GCTGGGACCC AGTGGGCCCT CTACCCCAGT CACACAAGCC GGCCTGTGGG CAGGACCACA 
GCTCCACCCA CCACACGCCA GCCTCCCACC ACTGCTCCCC GTCGCGTGCC CGGACGCCGG 
CCCTTGCCCC CAGGCACCCA GCAGCCCCCG AAGCCCTGCG ACTCCCAGCC CTGCCTCCAT 
GGGGGAACCT GCCAGGACCA GGACTCGGGT GGAGGCTTCA CCTGCAGCTG CCCAGTGGGC 
AGGAGGGGTG CCGTCTGTGA GAAGGCCCTG TACCTCTCGG TGCCAGCTTT CAATGGCCGC 
TCCTTCCTGG CCTTCCCCAC CCTCCGTGCC TACCACACGC TGCGCCTAGC ACTTGAGTTC 
CGGGCACTAG AGCCACAGGG CCTGCTGCTC TATAATGGCA ATGCCCAGGG CAAGGACTTC 
CTGGCATTGA CGCTGCTGGG GGGCCGTGTG CAGCTCAGGT TTGACACAGG TTCCGGGCCT 
GCAGTACTGA CCAGCTCAGT GCCTATAGAG CCTGGCCGCT GGCATCGCTT GGAGCTGTCT 
CGGCACTGGC GCCGGGGAAC ACTTTCAGTG GATGGTGAGC CCCTGGTGCT GGGCCACAGC 
CCCAGCGGTA CAGATGGTCT CAACCTGGAC ACAGACCTTT TTGTGGGCGG CATCCCTGAG 
GATCAGGCTT CTGTGGTACT CGAAAGGACG TCCATCAGCG TTGGCCTCAA GGGCTGCATC 
CGCCTGCTGG ATGTCAATAA TCAGCGTTTA GAGCTCAGCA GCTGGCCAGG GGCTGCAGCC 
CGAAGCTCCG GGGTGGGCGA GTGTGGAGAC CACCCCTGCC TGCCCAACCC CTGCCTCGAC 
GGGGCTCCGT GCCAGGCCCT GGAGGCTGGC ATGTTCCACT GTCAGTGCCC ACCTGGCCGC 
TTTGGTCCAA CCTGTAGTGG TGACAAGAAC CCCTGTGAGC CGAACCCCTG CCATGGGGCA 
GCCCCCTGCC GTGTGCTGCC TGATGGGGAG GCCAAGTGTG AATGCCCCCG AGGACGAGGG 
GGTGCCATCT GCCAGACAGT CTCGGAGCGG GATTCTTCCC AGCCCTTCCT GGCTGACTTC 
CATGGCTTCT CCTACCTGGA GCTGAAAGGG TTGCATACCT TTGAGCGGGA CCTGGGGGAG 
AAGCTGGCAC TGGAGGTGGT GTTCCTGGCC CGTGGGCCCA GTGGTTTGCT CCTCTACAAT 
GGGCAGAAAA CAGACGGCAA GGGGGACTTT GTGTCACTGG CGTTGCATGA CCGCTTCTTG 
GAATTCCGCT ATGACCTGGG CAAGGGAGCA GCGGTCATCA GGAGCAAGGA GCCGGTGGCC 
CTGGACACCT GGACCAGGGT CTCACTAGAG CGCAATGGCC GCAAGGGTGC CATGCGTGTC 
AGTGATGGGC CTCGCGTGCT GGGAGAGTCC CCGAAATCCC GCAAGGTGCC ACACACTGTC 
CTCAACCTGA AGGAGCCACT CTATGTTGGA GGGGCCCCTG ACTTCAGCAA ACTGGCCCGG 
GCAGCTGCGG TATTCTCTGG CTTCGATGGC GCCATCCAAC TGGTCTCCTT GAAAGGCCAC 
CAGCTGCTGT CCCAGGAGCA CGTGGTGCGG TCAGTGGATG TCTCATCTTT TGCCCACCAC 
CCTTGTACTC AGGCCTCGGG CCACCCATGC CTCAATGGGG CCTCCTGCCT CCCTCGAGAG 
GCCTCCTATG AGTGCCTGTG CCCCGGGGGC TTCTCAGGGC TTCACTGTGA GAAGGGGCTG 
ATTGAGAAGT CAGCAGGGGA TGTGGACGCC CTGGCCTTTG ACGGACAGAC CTACGTCGAG 
TACCTCAATG CCGTGACTGA GAGCGAACTG ACCAATGAGA TCCCAGCTCC TGATGCTCCG 
GATTCCGGGG CCCTTCCCAG TGAGAAGGCC CTGCAGAGCA ACCACTTTGA GCTGAGCCTG 
CGCACGGAAG CCACTCAGGG GCTGGTGCTG TGGAGCGGCA AGGCCACGGA GCGGGCCGAC 
TATGTCGCAC TGGCCATCGT GGATGGGCGC CTGCAGCTGA CCTATGACCT GGGCTCCCAG 
CCCGTCGTGC TGCGCTCCAC CGTGCCGGTC AACACCAACC GCTGGCTACG AGTCAGGGCA 
CATAGGGAAC AGAGGGAAGG TTCCCTTCAG GTGGGCAATG AGGCTCCCGT GACTGGCTCT 
TCCCCGCTGG GTGCCACACA GCTAGACACA GACGGAACTC TATGGCTGGG TGGCCTGGAG 
AAGCTGCCCA TAGGCCAGGC CCTGCCCAAG GCCTATGGCA CGGGCTTTGT GGGCTGCCTG 
CGGGATGTGG TGGTGGGGCA GCGCCCACTG CACCTGCTGG AGGATGCTGT CACCAAGCCA 
GAGCTCCGAC CCTGTGCCGC CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620422.1
CDS	255..6161
Translation	MPPPPLERDPRQQRGVSLLVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620365.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620365.3

ORF Insert Sequence:

ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC 
TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC 
CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC 
AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG 
GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG 
CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT 
GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC 
CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT 
GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG 
CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC 
AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT 
CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT 
AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT 
GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG 
TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG 
GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT 
GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG 
ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG 
TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC 
ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA 
GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA 
CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC 
CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG 
CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT 
GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT 
GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA 
GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC 
TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC 
CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG 
TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT 
CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC 
TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA 
TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC 
CTTGGCCCGT GCCAGGACTC TGTTCTCCTT GCAGAGACTG TTGCTCCTGG GGCCCGCCCA 
ACATCTGTAC CCAGGGCCAC CTCAGGGCTC AGCACGAGCA AGGCGCTGCC ACCCGCCCCC 
AGCACCCTCC CCCTGGCTCC CAGCAGCACC CCTCATAGCC GGCCCACGCC TCGACCCTCA 
CCAGGATCTC GGACCACTGC CAGCATTCCC AGGACTGTCA CGAGGCCTGT GCTGACCATG 
CCCCCCACAG CCCCCGCCAC GGCAGCCAAC CTCGCCACAT CTGCTTTTGG CGAGTCTGGC 
AGTGCTGACG GGAGCGGCGA TGAGGGGCTG AGTGGTGACC TGGAGGCCAG TGGGACTGGC 
TCAGGGGGAC TCGAGCCCCC TGAAGGTGGC AGTGCTGTGA CCCCTGAGCC ACCCATGGAG 
AGGGCTTCTT GCTACAACTC GCCTTTGGGC TGCTGCTCGG ATGGCAAGAC GCCCTCGCTG 
GACGCAGAGG GTTCCAACTG TCCTGCCACC AAGGCGTTCC AGGGTGTGTT GGAGCTTGAG 
GGCGTGGAGG GGCAAGAGCT GTTCTACACG CCGGAGATGG CTGACCCCAA GTCAGAGCTT 
TTTGGGGAGA CTGCTAGGAG CATCGAGAGC ACCCTGGATG ACCTCTTCCG GAACTCAGAG 
GTTAAGAAGG ACTTCCGAAG CGTCCGGCTG AGGGACTTGG GGCCTGGCAG CTCAGTCCGA 
GCCATTGTGG ATGTGCACTT TGACCCTGCC ACAACCTTTA GGGCGCCTGA TGTGGGCCAA 
GCCCTGCTCC GGCAGATACA GGCATCCCGG CGCCGGTCCC TGGGCGTACG GCGGCCACTG 
CAGGAGCACG TGCGCTTTAT GGACTTTGAC TGGTTTCCTG CGTTCTTCAC GGGAGCCACC 
ACAGGAGCAA CACCCGCCGC AGCCACGGCC AGAGCCACCA CGGTGGCCCA CCTGCCACCT 
GCTGGGACCC AGTGGGCCCT CTACCCCAGT CACACAAGCC GGCCTGTGGG CAGGACCACA 
GCTCCACCCA CCACACGCCA GCCTCCCACC ACTGCTCCCC GTCGCGTGCC CGGACGCCGG 
CCCTTGCCCC CAGGCACCCA GCAGCCCCCG AAGCCCTGCG ACTCCCAGCC CTGCCTCCAT 
GGGGGAACCT GCCAGGACCA GGACTCGGGT GGAGGCTTCA CCTGCAGCTG CCCAGTGGGC 
AGGAGGGGTG CCGTCTGTGA GAAGGCCCTG TACCTCTCGG TGCCAGCTTT CAATGGCCGC 
TCCTTCCTGG CCTTCCCCAC CCTCCGTGCC TACCACACGC TGCGCCTAGC ACTTGAGTTC 
CGGGCACTAG AGCCACAGGG CCTGCTGCTC TATAATGGCA ATGCCCAGGG CAAGGACTTC 
CTGGCATTGA CGCTGCTGGG GGGCCGTGTG CAGCTCAGGT TTGACACAGG TTCCGGGCCT 
GCAGTACTGA CCAGCTCAGT GCCTATAGAG CCTGGCCGCT GGCATCGCTT GGAGCTGTCT 
CGGCACTGGC GCCGGGGAAC ACTTTCAGTG GATGGTGAGC CCCTGGTGCT GGGCCACAGC 
CCCAGCGGTA CAGATGGTCT CAACCTGGAC ACAGACCTTT TTGTGGGCGG CATCCCTGAG 
GATCAGGCTT CTGTGGTACT CGAAAGGACG TCCATCAGCG TTGGCCTCAA GGGCTGCATC 
CGCCTGCTGG ATGTCAATAA TCAGCGTTTA GAGCTCAGCA GCTGGCCAGG GGCTGCAGCC 
CGAAGCTCCG GGGTGGGCGA GTGTGGAGAC CACCCCTGCC TGCCCAACCC CTGCCTCGAC 
GGGGCTCCGT GCCAGGCCCT GGAGGCTGGC ATGTTCCACT GTCAGTGCCC ACCTGGCCGC 
TTTGGTCCAA CCTGTAGTGG TGACAAGAAC CCCTGTGAGC CGAACCCCTG CCATGGGGCA 
GCCCCCTGCC GTGTGCTGCC TGATGGGGAG GCCAAGTGTG AATGCCCCCG AGGACGAGGG 
GGTGCCATCT GCCAGACAGT CTCGGAGCGG GATTCTTCCC AGCCCTTCCT GGCTGACTTC 
CATGGCTTCT CCTACCTGGA GCTGAAAGGG TTGCATACCT TTGAGCGGGA CCTGGGGGAG 
AAGCTGGCAC TGGAGGTGGT GTTCCTGGCC CGTGGGCCCA GTGGTTTGCT CCTCTACAAT 
GGGCAGAAAA CAGACGGCAA GGGGGACTTT GTGTCACTGG CGTTGCATGA CCGCTTCTTG 
GAATTCCGCT ATGACCTGGG CAAGGGAGCA GCGGTCATCA GGAGCAAGGA GCCGGTGGCC 
CTGGACACCT GGACCAGGGT CTCACTAGAG CGCAATGGCC GCAAGGGTGC CATGCGTGTC 
AGTGATGGGC CTCGCGTGCT GGGAGAGTCC CCGAAATCCC GCAAGGTGCC ACACACTGTC 
CTCAACCTGA AGGAGCCACT CTATGTTGGA GGGGCCCCTG ACTTCAGCAA ACTGGCCCGG 
GCAGCTGCGG TATTCTCTGG CTTCGATGGC GCCATCCAAC TGGTCTCCTT GAAAGGCCAC 
CAGCTGCTGT CCCAGGAGCA CGTGGTGCGG TCAGTGGATG TCTCATCTTT TGCCCACCAC 
CCTTGTACTC AGGCCTCGGG CCACCCATGC CTCAATGGGG CCTCCTGCCT CCCTCGAGAG 
GCCTCCTATG AGTGCCTGTG CCCCGGGGGC TTCTCAGGGC TTCACTGTGA GAAGGGGCTG 
ATTGAGAAGT CAGCAGGGGA TGTGGACGCC CTGGCCTTTG ACGGACAGAC CTACGTCGAG 
TACCTCAATG CCGTGACTGA GAGCGAACTG ACCAATGAGA TCCCAGCTCC TGATGCTCCG 
GATTCCGGGG CCCTTCCCAG TGAGAAGGCC CTGCAGAGCA ACCACTTTGA GCTGAGCCTG 
CGCACGGAAG CCACTCAGGG GCTGGTGCTG TGGAGCGGCA AGGCCACGGA GCGGGCCGAC 
TATGTCGCAC TGGCCATCGT GGATGGGCGC CTGCAGCTGA CCTATGACCT GGGCTCCCAG 
CCCGTCGTGC TGCGCTCCAC CGTGCCGGTC AACACCAACC GCTGGCTACG AGTCAGGGCA 
CATAGGGAAC AGAGGGAAGG TTCCCTTCAG GTGGGCAATG AGGCTCCCGT GACTGGCTCT 
TCCCCGCTGG GTGCCACACA GCTAGACACA GACGGAACTC TATGGCTGGG TGGCCTGGAG 
AAGCTGCCCA TAGGCCAGGC CCTGCCCAAG GCCTATGGCA CGGGCTTTGT GGGCTGCCTG 
CGGGATGTGG TGGTGGGGCA GCGCCCACTG CACCTGCTGG AGGATGCTGT CACCAAGCCA 
GAGCTCCGAC CCTGTGCCGC CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620366.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620423.1 Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA.

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CloneID	OCe06701
Clone ID Related Accession (Same CDS sequence)	XM_005620366.2 , XM_005620366.3
Accession Version	XM_005620366.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agrin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC 
TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC 
CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC 
AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG 
GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG 
CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT 
GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC 
CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT 
GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG 
CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC 
AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT 
CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT 
AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT 
GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG 
TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG 
GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT 
GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG 
ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG 
TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC 
ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA 
GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA 
CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC 
CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG 
CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT 
GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT 
GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA 
GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC 
TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC 
CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG 
TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT 
CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC 
TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA 
TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC 
CTTGGCCCGT GCCAGGAGAC TGTTGCTCCT GGGGCCCGCC CAACATCTGT ACCCAGGGCC 
ACCTCAGGGC TCAGCACGAG CAAGGCGCTG CCACCCGCCC CCAGCACCCT CCCCCTGGCT 
CCCAGCAGCA CCCCTCATAG CCGGCCCACG CCTCGACCCT CACCAGGATC TCGGACCACT 
GCCAGCATTC CCAGGACTGT CACGAGGCCT GTGCTGACCA TGCCCCCCAC AGCCCCCGCC 
ACGGCAGCCA ACCTCGCCAC ATCTGCTTTT GGCGAGTCTG GCAGTGCTGA CGGGAGCGGC 
GATGAGGGGC TGAGTGGTGA CCTGGAGGCC AGTGGGACTG GCTCAGGGGG ACTCGAGCCC 
CCTGAAGGTG GCAGTGCTGT GACCCCTGAG CCACCCATGG AGAGGGCTTC TTGCTACAAC 
TCGCCTTTGG GCTGCTGCTC GGATGGCAAG ACGCCCTCGC TGGACGCAGA GGGTTCCAAC 
TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG TTGGAGCTTG AGGGCGTGGA GGGGCAAGAG 
CTGTTCTACA CGCCGGAGAT GGCTGACCCC AAGTCAGAGC TTTTTGGGGA GACTGCTAGG 
AGCATCGAGA GCACCCTGGA TGACCTCTTC CGGAACTCAG AGGTTAAGAA GGACTTCCGA 
AGCGTCCGGC TGAGGGACTT GGGGCCTGGC AGCTCAGTCC GAGCCATTGT GGATGTGCAC 
TTTGACCCTG CCACAACCTT TAGGGCGCCT GATGTGGGCC AAGCCCTGCT CCGGCAGATA 
CAGGCATCCC GGCGCCGGTC CCTGGGCGTA CGGCGGCCAC TGCAGGAGCA CGTGCGCTTT 
ATGGACTTTG ACTGGTTTCC TGCGTTCTTC ACGGGAGCCA CCACAGGAGC AACACCCGCC 
GCAGCCACGG CCAGAGCCAC CACGGTGGCC CACCTGCCAC CTGCTGGGAC CCAGTGGGCC 
CTCTACCCCA GTCACACAAG CCGGCCTGTG GGCAGGACCA CAGCTCCACC CACCACACGC 
CAGCCTCCCA CCACTGCTCC CCGTCGCGTG CCCGGACGCC GGCCCTTGCC CCCAGGCACC 
CAGCAGCCCC CGAAGCCCTG CGACTCCCAG CCCTGCCTCC ATGGGGGAAC CTGCCAGGAC 
CAGGACTCGG GTGGAGGCTT CACCTGCAGC TGCCCAGTGG GCAGGAGGGG TGCCGTCTGT 
GAGAAGGCCC TGTACCTCTC GGTGCCAGCT TTCAATGGCC GCTCCTTCCT GGCCTTCCCC 
ACCCTCCGTG CCTACCACAC GCTGCGCCTA GCACTTGAGT TCCGGGCACT AGAGCCACAG 
GGCCTGCTGC TCTATAATGG CAATGCCCAG GGCAAGGACT TCCTGGCATT GACGCTGCTG 
GGGGGCCGTG TGCAGCTCAG GTTTGACACA GGTTCCGGGC CTGCAGTACT GACCAGCTCA 
GTGCCTATAG AGCCTGGCCG CTGGCATCGC TTGGAGCTGT CTCGGCACTG GCGCCGGGGA 
ACACTTTCAG TGGATGGTGA GCCCCTGGTG CTGGGCCACA GCCCCAGCGG TACAGATGGT 
CTCAACCTGG ACACAGACCT TTTTGTGGGC GGCATCCCTG AGGATCAGGC TTCTGTGGTA 
CTCGAAAGGA CGTCCATCAG CGTTGGCCTC AAGGGCTGCA TCCGCCTGCT GGATGTCAAT 
AATCAGCGTT TAGAGCTCAG CAGCTGGCCA GGGGCTGCAG CCCGAAGCTC CGGGGTGGGC 
GAGTGTGGAG ACCACCCCTG CCTGCCCAAC CCCTGCCTCG ACGGGGCTCC GTGCCAGGCC 
CTGGAGGCTG GCATGTTCCA CTGTCAGTGC CCACCTGGCC GCTTTGGTCC AACCTGTAGT 
GGTGACAAGA ACCCCTGTGA GCCGAACCCC TGCCATGGGG CAGCCCCCTG CCGTGTGCTG 
CCTGATGGGG AGGCCAAGTG TGAATGCCCC CGAGGACGAG GGGGTGCCAT CTGCCAGACA 
GTCTCGGAGC GGGATTCTTC CCAGCCCTTC CTGGCTGACT TCCATGGCTT CTCCTACCTG 
GAGCTGAAAG GGTTGCATAC CTTTGAGCGG GACCTGGGGG AGAAGCTGGC ACTGGAGGTG 
GTGTTCCTGG CCCGTGGGCC CAGTGGTTTG CTCCTCTACA ATGGGCAGAA AACAGACGGC 
AAGGGGGACT TTGTGTCACT GGCGTTGCAT GACCGCTTCT TGGAATTCCG CTATGACCTG 
GGCAAGGGAG CAGCGGTCAT CAGGAGCAAG GAGCCGGTGG CCCTGGACAC CTGGACCAGG 
GTCTCACTAG AGCGCAATGG CCGCAAGGGT GCCATGCGTG TCAGTGATGG GCCTCGCGTG 
CTGGGAGAGT CCCCGAAATC CCGCAAGGTG CCACACACTG TCCTCAACCT GAAGGAGCCA 
CTCTATGTTG GAGGGGCCCC TGACTTCAGC AAACTGGCCC GGGCAGCTGC GGTATTCTCT 
GGCTTCGATG GCGCCATCCA ACTGGTCTCC TTGAAAGGCC ACCAGCTGCT GTCCCAGGAG 
CACGTGGTGC GGTCAGTGGA TGTCTCATCT TTTGCCCACC ACCCTTGTAC TCAGGCCTCG 
GGCCACCCAT GCCTCAATGG GGCCTCCTGC CTCCCTCGAG AGGCCTCCTA TGAGTGCCTG 
TGCCCCGGGG GCTTCTCAGG GCTTCACTGT GAGAAGGGGC TGATTGAGAA GTCAGCAGGG 
GATGTGGACG CCCTGGCCTT TGACGGACAG ACCTACGTCG AGTACCTCAA TGCCGTGACT 
GAGAGCGAAC TGACCAATGA GATCCCAGCT CCTGATGCTC CGGATTCCGG GGCCCTTCCC 
AGTGAGAAGG CCCTGCAGAG CAACCACTTT GAGCTGAGCC TGCGCACGGA AGCCACTCAG 
GGGCTGGTGC TGTGGAGCGG CAAGGCCACG GAGCGGGCCG ACTATGTCGC ACTGGCCATC 
GTGGATGGGC GCCTGCAGCT GACCTATGAC CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC 
ACCGTGCCGG TCAACACCAA CCGCTGGCTA CGAGTCAGGG CACATAGGGA ACAGAGGGAA 
GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC GTGACTGGCT CTTCCCCGCT GGGTGCCACA 
CAGCTAGACA CAGACGGAAC TCTATGGCTG GGTGGCCTGG AGAAGCTGCC CATAGGCCAG 
GCCCTGCCCA AGGCCTATGG CACGGGCTTT GTGGGCTGCC TGCGGGATGT GGTGGTGGGG 
CAGCGCCCAC TGCACCTGCT GGAGGATGCT GTCACCAAGC CAGAGCTCCG ACCCTGTGCC 
GCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620423.1
CDS	747..6635
Translation	MPPPPLERDPRQQRGVSLLVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620366.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620366.2

ORF Insert Sequence:

ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC 
TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC 
CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC 
AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG 
GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG 
CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT 
GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC 
CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT 
GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG 
CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC 
AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT 
CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT 
AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT 
GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG 
TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG 
GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT 
GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG 
ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG 
TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC 
ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA 
GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA 
CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC 
CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG 
CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT 
GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT 
GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA 
GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC 
TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC 
CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG 
TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT 
CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC 
TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA 
TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC 
CTTGGCCCGT GCCAGGAGAC TGTTGCTCCT GGGGCCCGCC CAACATCTGT ACCCAGGGCC 
ACCTCAGGGC TCAGCACGAG CAAGGCGCTG CCACCCGCCC CCAGCACCCT CCCCCTGGCT 
CCCAGCAGCA CCCCTCATAG CCGGCCCACG CCTCGACCCT CACCAGGATC TCGGACCACT 
GCCAGCATTC CCAGGACTGT CACGAGGCCT GTGCTGACCA TGCCCCCCAC AGCCCCCGCC 
ACGGCAGCCA ACCTCGCCAC ATCTGCTTTT GGCGAGTCTG GCAGTGCTGA CGGGAGCGGC 
GATGAGGGGC TGAGTGGTGA CCTGGAGGCC AGTGGGACTG GCTCAGGGGG ACTCGAGCCC 
CCTGAAGGTG GCAGTGCTGT GACCCCTGAG CCACCCATGG AGAGGGCTTC TTGCTACAAC 
TCGCCTTTGG GCTGCTGCTC GGATGGCAAG ACGCCCTCGC TGGACGCAGA GGGTTCCAAC 
TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG TTGGAGCTTG AGGGCGTGGA GGGGCAAGAG 
CTGTTCTACA CGCCGGAGAT GGCTGACCCC AAGTCAGAGC TTTTTGGGGA GACTGCTAGG 
AGCATCGAGA GCACCCTGGA TGACCTCTTC CGGAACTCAG AGGTTAAGAA GGACTTCCGA 
AGCGTCCGGC TGAGGGACTT GGGGCCTGGC AGCTCAGTCC GAGCCATTGT GGATGTGCAC 
TTTGACCCTG CCACAACCTT TAGGGCGCCT GATGTGGGCC AAGCCCTGCT CCGGCAGATA 
CAGGCATCCC GGCGCCGGTC CCTGGGCGTA CGGCGGCCAC TGCAGGAGCA CGTGCGCTTT 
ATGGACTTTG ACTGGTTTCC TGCGTTCTTC ACGGGAGCCA CCACAGGAGC AACACCCGCC 
GCAGCCACGG CCAGAGCCAC CACGGTGGCC CACCTGCCAC CTGCTGGGAC CCAGTGGGCC 
CTCTACCCCA GTCACACAAG CCGGCCTGTG GGCAGGACCA CAGCTCCACC CACCACACGC 
CAGCCTCCCA CCACTGCTCC CCGTCGCGTG CCCGGACGCC GGCCCTTGCC CCCAGGCACC 
CAGCAGCCCC CGAAGCCCTG CGACTCCCAG CCCTGCCTCC ATGGGGGAAC CTGCCAGGAC 
CAGGACTCGG GTGGAGGCTT CACCTGCAGC TGCCCAGTGG GCAGGAGGGG TGCCGTCTGT 
GAGAAGGCCC TGTACCTCTC GGTGCCAGCT TTCAATGGCC GCTCCTTCCT GGCCTTCCCC 
ACCCTCCGTG CCTACCACAC GCTGCGCCTA GCACTTGAGT TCCGGGCACT AGAGCCACAG 
GGCCTGCTGC TCTATAATGG CAATGCCCAG GGCAAGGACT TCCTGGCATT GACGCTGCTG 
GGGGGCCGTG TGCAGCTCAG GTTTGACACA GGTTCCGGGC CTGCAGTACT GACCAGCTCA 
GTGCCTATAG AGCCTGGCCG CTGGCATCGC TTGGAGCTGT CTCGGCACTG GCGCCGGGGA 
ACACTTTCAG TGGATGGTGA GCCCCTGGTG CTGGGCCACA GCCCCAGCGG TACAGATGGT 
CTCAACCTGG ACACAGACCT TTTTGTGGGC GGCATCCCTG AGGATCAGGC TTCTGTGGTA 
CTCGAAAGGA CGTCCATCAG CGTTGGCCTC AAGGGCTGCA TCCGCCTGCT GGATGTCAAT 
AATCAGCGTT TAGAGCTCAG CAGCTGGCCA GGGGCTGCAG CCCGAAGCTC CGGGGTGGGC 
GAGTGTGGAG ACCACCCCTG CCTGCCCAAC CCCTGCCTCG ACGGGGCTCC GTGCCAGGCC 
CTGGAGGCTG GCATGTTCCA CTGTCAGTGC CCACCTGGCC GCTTTGGTCC AACCTGTAGT 
GGTGACAAGA ACCCCTGTGA GCCGAACCCC TGCCATGGGG CAGCCCCCTG CCGTGTGCTG 
CCTGATGGGG AGGCCAAGTG TGAATGCCCC CGAGGACGAG GGGGTGCCAT CTGCCAGACA 
GTCTCGGAGC GGGATTCTTC CCAGCCCTTC CTGGCTGACT TCCATGGCTT CTCCTACCTG 
GAGCTGAAAG GGTTGCATAC CTTTGAGCGG GACCTGGGGG AGAAGCTGGC ACTGGAGGTG 
GTGTTCCTGG CCCGTGGGCC CAGTGGTTTG CTCCTCTACA ATGGGCAGAA AACAGACGGC 
AAGGGGGACT TTGTGTCACT GGCGTTGCAT GACCGCTTCT TGGAATTCCG CTATGACCTG 
GGCAAGGGAG CAGCGGTCAT CAGGAGCAAG GAGCCGGTGG CCCTGGACAC CTGGACCAGG 
GTCTCACTAG AGCGCAATGG CCGCAAGGGT GCCATGCGTG TCAGTGATGG GCCTCGCGTG 
CTGGGAGAGT CCCCGAAATC CCGCAAGGTG CCACACACTG TCCTCAACCT GAAGGAGCCA 
CTCTATGTTG GAGGGGCCCC TGACTTCAGC AAACTGGCCC GGGCAGCTGC GGTATTCTCT 
GGCTTCGATG GCGCCATCCA ACTGGTCTCC TTGAAAGGCC ACCAGCTGCT GTCCCAGGAG 
CACGTGGTGC GGTCAGTGGA TGTCTCATCT TTTGCCCACC ACCCTTGTAC TCAGGCCTCG 
GGCCACCCAT GCCTCAATGG GGCCTCCTGC CTCCCTCGAG AGGCCTCCTA TGAGTGCCTG 
TGCCCCGGGG GCTTCTCAGG GCTTCACTGT GAGAAGGGGC TGATTGAGAA GTCAGCAGGG 
GATGTGGACG CCCTGGCCTT TGACGGACAG ACCTACGTCG AGTACCTCAA TGCCGTGACT 
GAGAGCGAAC TGACCAATGA GATCCCAGCT CCTGATGCTC CGGATTCCGG GGCCCTTCCC 
AGTGAGAAGG CCCTGCAGAG CAACCACTTT GAGCTGAGCC TGCGCACGGA AGCCACTCAG 
GGGCTGGTGC TGTGGAGCGG CAAGGCCACG GAGCGGGCCG ACTATGTCGC ACTGGCCATC 
GTGGATGGGC GCCTGCAGCT GACCTATGAC CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC 
ACCGTGCCGG TCAACACCAA CCGCTGGCTA CGAGTCAGGG CACATAGGGA ACAGAGGGAA 
GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC GTGACTGGCT CTTCCCCGCT GGGTGCCACA 
CAGCTAGACA CAGACGGAAC TCTATGGCTG GGTGGCCTGG AGAAGCTGCC CATAGGCCAG 
GCCCTGCCCA AGGCCTATGG CACGGGCTTT GTGGGCTGCC TGCGGGATGT GGTGGTGGGG 
CAGCGCCCAC TGCACCTGCT GGAGGATGCT GTCACCAAGC CAGAGCTCCG ACCCTGTGCC 
GCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620366.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620423.1 Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA.

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CloneID	OCe06701
Clone ID Related Accession (Same CDS sequence)	XM_005620366.2 , XM_005620366.3
Accession Version	XM_005620366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agrin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620366.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC 
TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC 
CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC 
AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG 
GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG 
CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT 
GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC 
CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT 
GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG 
CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC 
AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT 
CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT 
AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT 
GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG 
TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG 
GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT 
GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG 
ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG 
TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC 
ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA 
GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA 
CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC 
CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG 
CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT 
GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT 
GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA 
GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC 
TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC 
CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG 
TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT 
CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC 
TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA 
TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC 
CTTGGCCCGT GCCAGGAGAC TGTTGCTCCT GGGGCCCGCC CAACATCTGT ACCCAGGGCC 
ACCTCAGGGC TCAGCACGAG CAAGGCGCTG CCACCCGCCC CCAGCACCCT CCCCCTGGCT 
CCCAGCAGCA CCCCTCATAG CCGGCCCACG CCTCGACCCT CACCAGGATC TCGGACCACT 
GCCAGCATTC CCAGGACTGT CACGAGGCCT GTGCTGACCA TGCCCCCCAC AGCCCCCGCC 
ACGGCAGCCA ACCTCGCCAC ATCTGCTTTT GGCGAGTCTG GCAGTGCTGA CGGGAGCGGC 
GATGAGGGGC TGAGTGGTGA CCTGGAGGCC AGTGGGACTG GCTCAGGGGG ACTCGAGCCC 
CCTGAAGGTG GCAGTGCTGT GACCCCTGAG CCACCCATGG AGAGGGCTTC TTGCTACAAC 
TCGCCTTTGG GCTGCTGCTC GGATGGCAAG ACGCCCTCGC TGGACGCAGA GGGTTCCAAC 
TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG TTGGAGCTTG AGGGCGTGGA GGGGCAAGAG 
CTGTTCTACA CGCCGGAGAT GGCTGACCCC AAGTCAGAGC TTTTTGGGGA GACTGCTAGG 
AGCATCGAGA GCACCCTGGA TGACCTCTTC CGGAACTCAG AGGTTAAGAA GGACTTCCGA 
AGCGTCCGGC TGAGGGACTT GGGGCCTGGC AGCTCAGTCC GAGCCATTGT GGATGTGCAC 
TTTGACCCTG CCACAACCTT TAGGGCGCCT GATGTGGGCC AAGCCCTGCT CCGGCAGATA 
CAGGCATCCC GGCGCCGGTC CCTGGGCGTA CGGCGGCCAC TGCAGGAGCA CGTGCGCTTT 
ATGGACTTTG ACTGGTTTCC TGCGTTCTTC ACGGGAGCCA CCACAGGAGC AACACCCGCC 
GCAGCCACGG CCAGAGCCAC CACGGTGGCC CACCTGCCAC CTGCTGGGAC CCAGTGGGCC 
CTCTACCCCA GTCACACAAG CCGGCCTGTG GGCAGGACCA CAGCTCCACC CACCACACGC 
CAGCCTCCCA CCACTGCTCC CCGTCGCGTG CCCGGACGCC GGCCCTTGCC CCCAGGCACC 
CAGCAGCCCC CGAAGCCCTG CGACTCCCAG CCCTGCCTCC ATGGGGGAAC CTGCCAGGAC 
CAGGACTCGG GTGGAGGCTT CACCTGCAGC TGCCCAGTGG GCAGGAGGGG TGCCGTCTGT 
GAGAAGGCCC TGTACCTCTC GGTGCCAGCT TTCAATGGCC GCTCCTTCCT GGCCTTCCCC 
ACCCTCCGTG CCTACCACAC GCTGCGCCTA GCACTTGAGT TCCGGGCACT AGAGCCACAG 
GGCCTGCTGC TCTATAATGG CAATGCCCAG GGCAAGGACT TCCTGGCATT GACGCTGCTG 
GGGGGCCGTG TGCAGCTCAG GTTTGACACA GGTTCCGGGC CTGCAGTACT GACCAGCTCA 
GTGCCTATAG AGCCTGGCCG CTGGCATCGC TTGGAGCTGT CTCGGCACTG GCGCCGGGGA 
ACACTTTCAG TGGATGGTGA GCCCCTGGTG CTGGGCCACA GCCCCAGCGG TACAGATGGT 
CTCAACCTGG ACACAGACCT TTTTGTGGGC GGCATCCCTG AGGATCAGGC TTCTGTGGTA 
CTCGAAAGGA CGTCCATCAG CGTTGGCCTC AAGGGCTGCA TCCGCCTGCT GGATGTCAAT 
AATCAGCGTT TAGAGCTCAG CAGCTGGCCA GGGGCTGCAG CCCGAAGCTC CGGGGTGGGC 
GAGTGTGGAG ACCACCCCTG CCTGCCCAAC CCCTGCCTCG ACGGGGCTCC GTGCCAGGCC 
CTGGAGGCTG GCATGTTCCA CTGTCAGTGC CCACCTGGCC GCTTTGGTCC AACCTGTAGT 
GGTGACAAGA ACCCCTGTGA GCCGAACCCC TGCCATGGGG CAGCCCCCTG CCGTGTGCTG 
CCTGATGGGG AGGCCAAGTG TGAATGCCCC CGAGGACGAG GGGGTGCCAT CTGCCAGACA 
GTCTCGGAGC GGGATTCTTC CCAGCCCTTC CTGGCTGACT TCCATGGCTT CTCCTACCTG 
GAGCTGAAAG GGTTGCATAC CTTTGAGCGG GACCTGGGGG AGAAGCTGGC ACTGGAGGTG 
GTGTTCCTGG CCCGTGGGCC CAGTGGTTTG CTCCTCTACA ATGGGCAGAA AACAGACGGC 
AAGGGGGACT TTGTGTCACT GGCGTTGCAT GACCGCTTCT TGGAATTCCG CTATGACCTG 
GGCAAGGGAG CAGCGGTCAT CAGGAGCAAG GAGCCGGTGG CCCTGGACAC CTGGACCAGG 
GTCTCACTAG AGCGCAATGG CCGCAAGGGT GCCATGCGTG TCAGTGATGG GCCTCGCGTG 
CTGGGAGAGT CCCCGAAATC CCGCAAGGTG CCACACACTG TCCTCAACCT GAAGGAGCCA 
CTCTATGTTG GAGGGGCCCC TGACTTCAGC AAACTGGCCC GGGCAGCTGC GGTATTCTCT 
GGCTTCGATG GCGCCATCCA ACTGGTCTCC TTGAAAGGCC ACCAGCTGCT GTCCCAGGAG 
CACGTGGTGC GGTCAGTGGA TGTCTCATCT TTTGCCCACC ACCCTTGTAC TCAGGCCTCG 
GGCCACCCAT GCCTCAATGG GGCCTCCTGC CTCCCTCGAG AGGCCTCCTA TGAGTGCCTG 
TGCCCCGGGG GCTTCTCAGG GCTTCACTGT GAGAAGGGGC TGATTGAGAA GTCAGCAGGG 
GATGTGGACG CCCTGGCCTT TGACGGACAG ACCTACGTCG AGTACCTCAA TGCCGTGACT 
GAGAGCGAAC TGACCAATGA GATCCCAGCT CCTGATGCTC CGGATTCCGG GGCCCTTCCC 
AGTGAGAAGG CCCTGCAGAG CAACCACTTT GAGCTGAGCC TGCGCACGGA AGCCACTCAG 
GGGCTGGTGC TGTGGAGCGG CAAGGCCACG GAGCGGGCCG ACTATGTCGC ACTGGCCATC 
GTGGATGGGC GCCTGCAGCT GACCTATGAC CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC 
ACCGTGCCGG TCAACACCAA CCGCTGGCTA CGAGTCAGGG CACATAGGGA ACAGAGGGAA 
GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC GTGACTGGCT CTTCCCCGCT GGGTGCCACA 
CAGCTAGACA CAGACGGAAC TCTATGGCTG GGTGGCCTGG AGAAGCTGCC CATAGGCCAG 
GCCCTGCCCA AGGCCTATGG CACGGGCTTT GTGGGCTGCC TGCGGGATGT GGTGGTGGGG 
CAGCGCCCAC TGCACCTGCT GGAGGATGCT GTCACCAAGC CAGAGCTCCG ACCCTGTGCC 
GCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620423.1
CDS	254..6142
Translation	MPPPPLERDPRQQRGVSLLVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620366.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620366.3

ORF Insert Sequence:

ATGCCTCCGC CACCTTTGGA GCGGGACCCC CGGCAGCAGC GTGGGGTCTC CCTGCTGGTG 
CGGTATTTTA TGATTCCCTG CAACGTCTGC CTGATTCTGC TGGCCACGTC CACGCTGGGC 
TTCGCGGTGC TGCTTTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCGCCC 
CCAACGCCTC CAGATGCATG CCGAGGAATG CTGTGTGGCT TCGGTGCTGT GTGTGAGCCC 
AGTGCAGGGG GTTCAGGCCG AGCCTCCTGC GTGTGCAAGA AGAGTGCCTG TCCCGCCGTG 
GTGGCGCCCG TGTGTGGCTC TGACGCCTCC ACCTACAGCA ATGAGTGTGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTACTCAGTC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC ATGCAGCTTC GGCAGCACCT GTGTGCGCTC AGCAGATGGG 
CAGACAGCCA CATGCCTGTG CCCTGCCACC TGCCGGGGGC CCCCGGAGGG CCCCGTGTGT 
GGGAGCGACG GTGCTGACTA CCCCAGTGAG TGCCAGCTCC TGCGCCTCGC GTGCACTCGC 
CAGGAGAACA TCGTCAAGAA GTTTGACGGC CCTTGTGACC CCTGCCAGGG CACCCACCAT 
GACCTGAGCC GTGTCTGCCG CGTTCACCCA CGCACGCGGC ATCCTGAGAT GCTCCTCCGG 
CCAGAGAGTT GCCCTTCTCA GCCAGCACCT GTGTGCGGGG ATGATGGAGT CACCTATGAC 
AACGACTGCA TAATGGGCCG CACGGGAGCC ACCCGTGGCC TTGTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCA AGACCAGTGT CCAGATGTGT GCAGGTTCAA TGCTGTGTGT 
CTGTCCCGCC GAGGCCGCCC CCGCTGCTCC TGTGACCGTG TCATCTGTGA TGGGGCTTAT 
AGACCCGTGT GTGCTCACGA CGGGCACACG TATGATAATG ACTGCTGGCG TCAGCAAGCT 
GAGTGTCGGC AGCAGCGCAC CATTCCCGCC AAGCACCAGG GCCCGTGTGA CCAGCCCCTG 
TCCCCGTGCC TTGGGGTGCG GTGTCCTTTT GGGGCAACAT GTGCCGTGAA GAATGGGGAG 
GCTGAGTGCG TGTGCCAGCA GGTGTGTTCG GGCATCTATG ATCCTGTGTG TGGCAGTGAT 
GGTGTCACAT ATGGCAGCAC ATGTGAACTG GAAGCCACGG CCTGCGCCCT TGGACGGGAG 
ATCCGGGTGG CTCGCAGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCTCTG 
TGTGAGGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAGT GCGTGGCCTC GGCTCAACCT 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCACGT CCACGCCTGC 
ACACACCAGA TCAGCCTGCA TGTGGTCTCA GCTGGACACT GCCAGACCTG CGGAGACACA 
GTGTGTGCTT TCGGGGCAGT GTGCTTGGCT GGGCAATGTG TGTGTCCCCG ATGTGAGCGA 
CCCCCACCCG GGCCCGTGTG TGGCAGCGAT GGTGTCACCT ACGACAGTGC CTGTCATCTC 
CGAGAAGCTG CCTGCCAGCA ACAGACACAG ATTGAGGAGG CCCGGGCGGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCTGG AGGCTCTGGC TCCGGAGAGG ATGGCGAGTG TGAGCAGGAG 
CTGTGCCAGC AGCATGGCGG TGTCTGGGAT GAGGACTCGG AAGATGGGCC TTGCGTCTGT 
GACTTCAGCT GCCATAGCGT CCTGAGGAGC CCGGTCTGTG GCTCAGATGG GCTCACCTAT 
GGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAATCGC GGCGAGAACT GTACGTCACA 
GCACAGGGAG CCTGCCGTGG CCCCACCTTG GCCCCATTGC TACCTGCAGT TCCCCCACAC 
TGCACCCAGA CCCCCTATGG CTGCTGCCAG GACAACGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCTC CTGCCAATGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAACTA CGGGTCAGTG CTCTTGCCGC CCAGGCGTCG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACTG ATGGCCGGAG CGGCTGCACC 
CCCTGCAGCT GTGACCCCCG GGGCGCTGTG CGGGATGACT GCGAGCAGAT GACCGGGCTG 
TGTTCATGTA AGCCTGGGGT GGCTGGACCT AAGTGCGGGC AGTGTCCAGA TGGCCACGCT 
CTGGGCCCTG GCGGCTGTGA AACAGACTCC TTGGCGCCTG AGACGTGTGC TGAGATGCAC 
TGTGAGTTTG GGGCGTCCTG CGTGGAGGAG GCTGGCTCTG CACGCTGTGT GTGCCCAACA 
TCTACCTGTC CAGGGGCCAG TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACCTACGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGACATCTC CATCCAGAGC 
CTTGGCCCGT GCCAGGAGAC TGTTGCTCCT GGGGCCCGCC CAACATCTGT ACCCAGGGCC 
ACCTCAGGGC TCAGCACGAG CAAGGCGCTG CCACCCGCCC CCAGCACCCT CCCCCTGGCT 
CCCAGCAGCA CCCCTCATAG CCGGCCCACG CCTCGACCCT CACCAGGATC TCGGACCACT 
GCCAGCATTC CCAGGACTGT CACGAGGCCT GTGCTGACCA TGCCCCCCAC AGCCCCCGCC 
ACGGCAGCCA ACCTCGCCAC ATCTGCTTTT GGCGAGTCTG GCAGTGCTGA CGGGAGCGGC 
GATGAGGGGC TGAGTGGTGA CCTGGAGGCC AGTGGGACTG GCTCAGGGGG ACTCGAGCCC 
CCTGAAGGTG GCAGTGCTGT GACCCCTGAG CCACCCATGG AGAGGGCTTC TTGCTACAAC 
TCGCCTTTGG GCTGCTGCTC GGATGGCAAG ACGCCCTCGC TGGACGCAGA GGGTTCCAAC 
TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG TTGGAGCTTG AGGGCGTGGA GGGGCAAGAG 
CTGTTCTACA CGCCGGAGAT GGCTGACCCC AAGTCAGAGC TTTTTGGGGA GACTGCTAGG 
AGCATCGAGA GCACCCTGGA TGACCTCTTC CGGAACTCAG AGGTTAAGAA GGACTTCCGA 
AGCGTCCGGC TGAGGGACTT GGGGCCTGGC AGCTCAGTCC GAGCCATTGT GGATGTGCAC 
TTTGACCCTG CCACAACCTT TAGGGCGCCT GATGTGGGCC AAGCCCTGCT CCGGCAGATA 
CAGGCATCCC GGCGCCGGTC CCTGGGCGTA CGGCGGCCAC TGCAGGAGCA CGTGCGCTTT 
ATGGACTTTG ACTGGTTTCC TGCGTTCTTC ACGGGAGCCA CCACAGGAGC AACACCCGCC 
GCAGCCACGG CCAGAGCCAC CACGGTGGCC CACCTGCCAC CTGCTGGGAC CCAGTGGGCC 
CTCTACCCCA GTCACACAAG CCGGCCTGTG GGCAGGACCA CAGCTCCACC CACCACACGC 
CAGCCTCCCA CCACTGCTCC CCGTCGCGTG CCCGGACGCC GGCCCTTGCC CCCAGGCACC 
CAGCAGCCCC CGAAGCCCTG CGACTCCCAG CCCTGCCTCC ATGGGGGAAC CTGCCAGGAC 
CAGGACTCGG GTGGAGGCTT CACCTGCAGC TGCCCAGTGG GCAGGAGGGG TGCCGTCTGT 
GAGAAGGCCC TGTACCTCTC GGTGCCAGCT TTCAATGGCC GCTCCTTCCT GGCCTTCCCC 
ACCCTCCGTG CCTACCACAC GCTGCGCCTA GCACTTGAGT TCCGGGCACT AGAGCCACAG 
GGCCTGCTGC TCTATAATGG CAATGCCCAG GGCAAGGACT TCCTGGCATT GACGCTGCTG 
GGGGGCCGTG TGCAGCTCAG GTTTGACACA GGTTCCGGGC CTGCAGTACT GACCAGCTCA 
GTGCCTATAG AGCCTGGCCG CTGGCATCGC TTGGAGCTGT CTCGGCACTG GCGCCGGGGA 
ACACTTTCAG TGGATGGTGA GCCCCTGGTG CTGGGCCACA GCCCCAGCGG TACAGATGGT 
CTCAACCTGG ACACAGACCT TTTTGTGGGC GGCATCCCTG AGGATCAGGC TTCTGTGGTA 
CTCGAAAGGA CGTCCATCAG CGTTGGCCTC AAGGGCTGCA TCCGCCTGCT GGATGTCAAT 
AATCAGCGTT TAGAGCTCAG CAGCTGGCCA GGGGCTGCAG CCCGAAGCTC CGGGGTGGGC 
GAGTGTGGAG ACCACCCCTG CCTGCCCAAC CCCTGCCTCG ACGGGGCTCC GTGCCAGGCC 
CTGGAGGCTG GCATGTTCCA CTGTCAGTGC CCACCTGGCC GCTTTGGTCC AACCTGTAGT 
GGTGACAAGA ACCCCTGTGA GCCGAACCCC TGCCATGGGG CAGCCCCCTG CCGTGTGCTG 
CCTGATGGGG AGGCCAAGTG TGAATGCCCC CGAGGACGAG GGGGTGCCAT CTGCCAGACA 
GTCTCGGAGC GGGATTCTTC CCAGCCCTTC CTGGCTGACT TCCATGGCTT CTCCTACCTG 
GAGCTGAAAG GGTTGCATAC CTTTGAGCGG GACCTGGGGG AGAAGCTGGC ACTGGAGGTG 
GTGTTCCTGG CCCGTGGGCC CAGTGGTTTG CTCCTCTACA ATGGGCAGAA AACAGACGGC 
AAGGGGGACT TTGTGTCACT GGCGTTGCAT GACCGCTTCT TGGAATTCCG CTATGACCTG 
GGCAAGGGAG CAGCGGTCAT CAGGAGCAAG GAGCCGGTGG CCCTGGACAC CTGGACCAGG 
GTCTCACTAG AGCGCAATGG CCGCAAGGGT GCCATGCGTG TCAGTGATGG GCCTCGCGTG 
CTGGGAGAGT CCCCGAAATC CCGCAAGGTG CCACACACTG TCCTCAACCT GAAGGAGCCA 
CTCTATGTTG GAGGGGCCCC TGACTTCAGC AAACTGGCCC GGGCAGCTGC GGTATTCTCT 
GGCTTCGATG GCGCCATCCA ACTGGTCTCC TTGAAAGGCC ACCAGCTGCT GTCCCAGGAG 
CACGTGGTGC GGTCAGTGGA TGTCTCATCT TTTGCCCACC ACCCTTGTAC TCAGGCCTCG 
GGCCACCCAT GCCTCAATGG GGCCTCCTGC CTCCCTCGAG AGGCCTCCTA TGAGTGCCTG 
TGCCCCGGGG GCTTCTCAGG GCTTCACTGT GAGAAGGGGC TGATTGAGAA GTCAGCAGGG 
GATGTGGACG CCCTGGCCTT TGACGGACAG ACCTACGTCG AGTACCTCAA TGCCGTGACT 
GAGAGCGAAC TGACCAATGA GATCCCAGCT CCTGATGCTC CGGATTCCGG GGCCCTTCCC 
AGTGAGAAGG CCCTGCAGAG CAACCACTTT GAGCTGAGCC TGCGCACGGA AGCCACTCAG 
GGGCTGGTGC TGTGGAGCGG CAAGGCCACG GAGCGGGCCG ACTATGTCGC ACTGGCCATC 
GTGGATGGGC GCCTGCAGCT GACCTATGAC CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC 
ACCGTGCCGG TCAACACCAA CCGCTGGCTA CGAGTCAGGG CACATAGGGA ACAGAGGGAA 
GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC GTGACTGGCT CTTCCCCGCT GGGTGCCACA 
CAGCTAGACA CAGACGGAAC TCTATGGCTG GGTGGCCTGG AGAAGCTGCC CATAGGCCAG 
GCCCTGCCCA AGGCCTATGG CACGGGCTTT GTGGGCTGCC TGCGGGATGT GGTGGTGGGG 
CAGCGCCCAC TGCACCTGCT GGAGGATGCT GTCACCAAGC CAGAGCTCCG ACCCTGTGCC 
GCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620359.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620416.1 Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA.

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CloneID	OCe06693
Clone ID Related Accession (Same CDS sequence)	XM_005620359.2 , XM_005620359.3
Accession Version	XM_005620359.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agrin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG 
ATCCCAGCTC CTGATGCTCC GGATTCCGGG GCCCTTCCCA GTGAGAAGGC CCTGCAGAGC 
AACCACTTTG AGCTGAGCCT GCGCACGGAA GCCACTCAGG GGCTGGTGCT GTGGAGCGGC 
AAGGCCACGG AGCGGGCCGA CTATGTCGCA CTGGCCATCG TGGATGGGCG CCTGCAGCTG 
ACCTATGACC TGGGCTCCCA GCCCGTCGTG CTGCGCTCCA CCGTGCCGGT CAACACCAAC 
CGCTGGCTAC GAGTCAGGGC ACATAGGGAA CAGAGGGAAG GTTCCCTTCA GGTGGGCAAT 
GAGGCTCCCG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AGCTAGACAC AGACGGAACT 
CTATGGCTGG GTGGCCTGGA GAAGCTGCCC ATAGGCCAGG CCCTGCCCAA GGCCTATGGC 
ACGGGCTTTG TGGGCTGCCT GCGGGATGTG GTGGTGGGGC AGCGCCCACT GCACCTGCTG 
GAGGATGCTG TCACCAAGCC AGAGCTCCGA CCCTGTGCCG CCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620416.1
CDS	133..6360
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620359.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620359.2

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG 
ATCCCAGCTC CTGATGCTCC GGATTCCGGG GCCCTTCCCA GTGAGAAGGC CCTGCAGAGC 
AACCACTTTG AGCTGAGCCT GCGCACGGAA GCCACTCAGG GGCTGGTGCT GTGGAGCGGC 
AAGGCCACGG AGCGGGCCGA CTATGTCGCA CTGGCCATCG TGGATGGGCG CCTGCAGCTG 
ACCTATGACC TGGGCTCCCA GCCCGTCGTG CTGCGCTCCA CCGTGCCGGT CAACACCAAC 
CGCTGGCTAC GAGTCAGGGC ACATAGGGAA CAGAGGGAAG GTTCCCTTCA GGTGGGCAAT 
GAGGCTCCCG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AGCTAGACAC AGACGGAACT 
CTATGGCTGG GTGGCCTGGA GAAGCTGCCC ATAGGCCAGG CCCTGCCCAA GGCCTATGGC 
ACGGGCTTTG TGGGCTGCCT GCGGGATGTG GTGGTGGGGC AGCGCCCACT GCACCTGCTG 
GAGGATGCTG TCACCAAGCC AGAGCTCCGA CCCTGTGCCG CCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620359.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620416.1 Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA.

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CloneID	OCe06693
Clone ID Related Accession (Same CDS sequence)	XM_005620359.2 , XM_005620359.3
Accession Version	XM_005620359.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG 
ATCCCAGCTC CTGATGCTCC GGATTCCGGG GCCCTTCCCA GTGAGAAGGC CCTGCAGAGC 
AACCACTTTG AGCTGAGCCT GCGCACGGAA GCCACTCAGG GGCTGGTGCT GTGGAGCGGC 
AAGGCCACGG AGCGGGCCGA CTATGTCGCA CTGGCCATCG TGGATGGGCG CCTGCAGCTG 
ACCTATGACC TGGGCTCCCA GCCCGTCGTG CTGCGCTCCA CCGTGCCGGT CAACACCAAC 
CGCTGGCTAC GAGTCAGGGC ACATAGGGAA CAGAGGGAAG GTTCCCTTCA GGTGGGCAAT 
GAGGCTCCCG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AGCTAGACAC AGACGGAACT 
CTATGGCTGG GTGGCCTGGA GAAGCTGCCC ATAGGCCAGG CCCTGCCCAA GGCCTATGGC 
ACGGGCTTTG TGGGCTGCCT GCGGGATGTG GTGGTGGGGC AGCGCCCACT GCACCTGCTG 
GAGGATGCTG TCACCAAGCC AGAGCTCCGA CCCTGTGCCG CCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620416.1
CDS	81..6308
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620359.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620359.3

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG 
ATCCCAGCTC CTGATGCTCC GGATTCCGGG GCCCTTCCCA GTGAGAAGGC CCTGCAGAGC 
AACCACTTTG AGCTGAGCCT GCGCACGGAA GCCACTCAGG GGCTGGTGCT GTGGAGCGGC 
AAGGCCACGG AGCGGGCCGA CTATGTCGCA CTGGCCATCG TGGATGGGCG CCTGCAGCTG 
ACCTATGACC TGGGCTCCCA GCCCGTCGTG CTGCGCTCCA CCGTGCCGGT CAACACCAAC 
CGCTGGCTAC GAGTCAGGGC ACATAGGGAA CAGAGGGAAG GTTCCCTTCA GGTGGGCAAT 
GAGGCTCCCG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AGCTAGACAC AGACGGAACT 
CTATGGCTGG GTGGCCTGGA GAAGCTGCCC ATAGGCCAGG CCCTGCCCAA GGCCTATGGC 
ACGGGCTTTG TGGGCTGCCT GCGGGATGTG GTGGTGGGGC AGCGCCCACT GCACCTGCTG 
GAGGATGCTG TCACCAAGCC AGAGCTCCGA CCCTGTGCCG CCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620360.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620417.1 Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA.

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CloneID	OCe06694
Clone ID Related Accession (Same CDS sequence)	XM_005620360.3 , XM_005620360.2
Accession Version	XM_005620360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA 
CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA 
CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG 
AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT 
GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC 
CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG 
AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC 
TACGTCGAGT ACCTCAATGC CGTGACTGAG AGCGAACTGA CCAATGAGAT CCCAGCTCCT 
GATGCTCCGG ATTCCGGGGC CCTTCCCAGT GAGAAGGCCC TGCAGAGCAA CCACTTTGAG 
CTGAGCCTGC GCACGGAAGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACGGAG 
CGGGCCGACT ATGTCGCACT GGCCATCGTG GATGGGCGCC TGCAGCTGAC CTATGACCTG 
GGCTCCCAGC CCGTCGTGCT GCGCTCCACC GTGCCGGTCA ACACCAACCG CTGGCTACGA 
GTCAGGGCAC ATAGGGAACA GAGGGAAGGT TCCCTTCAGG TGGGCAATGA GGCTCCCGTG 
ACTGGCTCTT CCCCGCTGGG TGCCACACAG CTAGACACAG ACGGAACTCT ATGGCTGGGT 
GGCCTGGAGA AGCTGCCCAT AGGCCAGGCC CTGCCCAAGG CCTATGGCAC GGGCTTTGTG 
GGCTGCCTGC GGGATGTGGT GGTGGGGCAG CGCCCACTGC ACCTGCTGGA GGATGCTGTC 
ACCAAGCCAG AGCTCCGACC CTGTGCCGCC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620417.1
CDS	81..6296
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620360.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620360.3

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA 
CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA 
CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG 
AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT 
GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC 
CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG 
AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC 
TACGTCGAGT ACCTCAATGC CGTGACTGAG AGCGAACTGA CCAATGAGAT CCCAGCTCCT 
GATGCTCCGG ATTCCGGGGC CCTTCCCAGT GAGAAGGCCC TGCAGAGCAA CCACTTTGAG 
CTGAGCCTGC GCACGGAAGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACGGAG 
CGGGCCGACT ATGTCGCACT GGCCATCGTG GATGGGCGCC TGCAGCTGAC CTATGACCTG 
GGCTCCCAGC CCGTCGTGCT GCGCTCCACC GTGCCGGTCA ACACCAACCG CTGGCTACGA 
GTCAGGGCAC ATAGGGAACA GAGGGAAGGT TCCCTTCAGG TGGGCAATGA GGCTCCCGTG 
ACTGGCTCTT CCCCGCTGGG TGCCACACAG CTAGACACAG ACGGAACTCT ATGGCTGGGT 
GGCCTGGAGA AGCTGCCCAT AGGCCAGGCC CTGCCCAAGG CCTATGGCAC GGGCTTTGTG 
GGCTGCCTGC GGGATGTGGT GGTGGGGCAG CGCCCACTGC ACCTGCTGGA GGATGCTGTC 
ACCAAGCCAG AGCTCCGACC CTGTGCCGCC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620360.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620417.1 Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA.

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CloneID	OCe06694
Clone ID Related Accession (Same CDS sequence)	XM_005620360.3 , XM_005620360.2
Accession Version	XM_005620360.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agrin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA 
CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA 
CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG 
AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT 
GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC 
CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG 
AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC 
TACGTCGAGT ACCTCAATGC CGTGACTGAG AGCGAACTGA CCAATGAGAT CCCAGCTCCT 
GATGCTCCGG ATTCCGGGGC CCTTCCCAGT GAGAAGGCCC TGCAGAGCAA CCACTTTGAG 
CTGAGCCTGC GCACGGAAGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACGGAG 
CGGGCCGACT ATGTCGCACT GGCCATCGTG GATGGGCGCC TGCAGCTGAC CTATGACCTG 
GGCTCCCAGC CCGTCGTGCT GCGCTCCACC GTGCCGGTCA ACACCAACCG CTGGCTACGA 
GTCAGGGCAC ATAGGGAACA GAGGGAAGGT TCCCTTCAGG TGGGCAATGA GGCTCCCGTG 
ACTGGCTCTT CCCCGCTGGG TGCCACACAG CTAGACACAG ACGGAACTCT ATGGCTGGGT 
GGCCTGGAGA AGCTGCCCAT AGGCCAGGCC CTGCCCAAGG CCTATGGCAC GGGCTTTGTG 
GGCTGCCTGC GGGATGTGGT GGTGGGGCAG CGCCCACTGC ACCTGCTGGA GGATGCTGTC 
ACCAAGCCAG AGCTCCGACC CTGTGCCGCC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620417.1
CDS	133..6348
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620360.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620360.2

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA 
CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA 
CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG 
AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT 
GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC 
CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG 
AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC 
TACGTCGAGT ACCTCAATGC CGTGACTGAG AGCGAACTGA CCAATGAGAT CCCAGCTCCT 
GATGCTCCGG ATTCCGGGGC CCTTCCCAGT GAGAAGGCCC TGCAGAGCAA CCACTTTGAG 
CTGAGCCTGC GCACGGAAGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACGGAG 
CGGGCCGACT ATGTCGCACT GGCCATCGTG GATGGGCGCC TGCAGCTGAC CTATGACCTG 
GGCTCCCAGC CCGTCGTGCT GCGCTCCACC GTGCCGGTCA ACACCAACCG CTGGCTACGA 
GTCAGGGCAC ATAGGGAACA GAGGGAAGGT TCCCTTCAGG TGGGCAATGA GGCTCCCGTG 
ACTGGCTCTT CCCCGCTGGG TGCCACACAG CTAGACACAG ACGGAACTCT ATGGCTGGGT 
GGCCTGGAGA AGCTGCCCAT AGGCCAGGCC CTGCCCAAGG CCTATGGCAC GGGCTTTGTG 
GGCTGCCTGC GGGATGTGGT GGTGGGGCAG CGCCCACTGC ACCTGCTGGA GGATGCTGTC 
ACCAAGCCAG AGCTCCGACC CTGTGCCGCC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620361.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620418.1 Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA.

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CloneID	OCe06695
Clone ID Related Accession (Same CDS sequence)	XM_005620361.3 , XM_005620361.2
Accession Version	XM_005620361.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC 
CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC 
CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC 
TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC 
ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT 
GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT 
GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG 
GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG 
CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT 
GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG 
CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA 
GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC 
CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC 
CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA 
CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC 
ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA 
CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC 
ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC 
CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC 
CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG 
GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC 
CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG 
TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC 
TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG 
CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG 
TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC 
AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT 
GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC 
ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA 
GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC 
GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC 
CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG 
GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA 
GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC 
TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG 
GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC 
AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC 
TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG 
GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT 
GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGAAAT CCCGCAAGGT GCCACACACT 
GTCCTCAACC TGAAGGAGCC ACTCTATGTT GGAGGGGCCC CTGACTTCAG CAAACTGGCC 
CGGGCAGCTG CGGTATTCTC TGGCTTCGAT GGCGCCATCC AACTGGTCTC CTTGAAAGGC 
CACCAGCTGC TGTCCCAGGA GCACGTGGTG CGGTCAGTGG ATGTCTCATC TTTTGCCCAC 
CACCCTTGTA CTCAGGCCTC GGGCCACCCA TGCCTCAATG GGGCCTCCTG CCTCCCTCGA 
GAGGCCTCCT ATGAGTGCCT GTGCCCCGGG GGCTTCTCAG GGCTTCACTG TGAGAAGGGG 
CTGATTGAGA AGTCAGCAGG GGATGTGGAC GCCCTGGCCT TTGACGGACA GACCTACGTC 
GAGTACCTCA ATGCCGTGAC TGAGAGCGAA CTGACCAATG AGATCCCAGC TCCTGATGCT 
CCGGATTCCG GGGCCCTTCC CAGTGAGAAG GCCCTGCAGA GCAACCACTT TGAGCTGAGC 
CTGCGCACGG AAGCCACTCA GGGGCTGGTG CTGTGGAGCG GCAAGGCCAC GGAGCGGGCC 
GACTATGTCG CACTGGCCAT CGTGGATGGG CGCCTGCAGC TGACCTATGA CCTGGGCTCC 
CAGCCCGTCG TGCTGCGCTC CACCGTGCCG GTCAACACCA ACCGCTGGCT ACGAGTCAGG 
GCACATAGGG AACAGAGGGA AGGTTCCCTT CAGGTGGGCA ATGAGGCTCC CGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAGCTAGAC ACAGACGGAA CTCTATGGCT GGGTGGCCTG 
GAGAAGCTGC CCATAGGCCA GGCCCTGCCC AAGGCCTATG GCACGGGCTT TGTGGGCTGC 
CTGCGGGATG TGGTGGTGGG GCAGCGCCCA CTGCACCTGC TGGAGGATGC TGTCACCAAG 
CCAGAGCTCC GACCCTGTGC CGCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620418.1
CDS	1..6210
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620361.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620361.3

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC 
CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC 
CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC 
TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC 
ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT 
GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT 
GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG 
GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG 
CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT 
GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG 
CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA 
GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC 
CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC 
CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA 
CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC 
ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA 
CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC 
ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC 
CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC 
CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG 
GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC 
CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG 
TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC 
TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG 
CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG 
TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC 
AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT 
GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC 
ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA 
GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC 
GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC 
CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG 
GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA 
GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC 
TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG 
GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC 
AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC 
TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG 
GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT 
GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGAAAT CCCGCAAGGT GCCACACACT 
GTCCTCAACC TGAAGGAGCC ACTCTATGTT GGAGGGGCCC CTGACTTCAG CAAACTGGCC 
CGGGCAGCTG CGGTATTCTC TGGCTTCGAT GGCGCCATCC AACTGGTCTC CTTGAAAGGC 
CACCAGCTGC TGTCCCAGGA GCACGTGGTG CGGTCAGTGG ATGTCTCATC TTTTGCCCAC 
CACCCTTGTA CTCAGGCCTC GGGCCACCCA TGCCTCAATG GGGCCTCCTG CCTCCCTCGA 
GAGGCCTCCT ATGAGTGCCT GTGCCCCGGG GGCTTCTCAG GGCTTCACTG TGAGAAGGGG 
CTGATTGAGA AGTCAGCAGG GGATGTGGAC GCCCTGGCCT TTGACGGACA GACCTACGTC 
GAGTACCTCA ATGCCGTGAC TGAGAGCGAA CTGACCAATG AGATCCCAGC TCCTGATGCT 
CCGGATTCCG GGGCCCTTCC CAGTGAGAAG GCCCTGCAGA GCAACCACTT TGAGCTGAGC 
CTGCGCACGG AAGCCACTCA GGGGCTGGTG CTGTGGAGCG GCAAGGCCAC GGAGCGGGCC 
GACTATGTCG CACTGGCCAT CGTGGATGGG CGCCTGCAGC TGACCTATGA CCTGGGCTCC 
CAGCCCGTCG TGCTGCGCTC CACCGTGCCG GTCAACACCA ACCGCTGGCT ACGAGTCAGG 
GCACATAGGG AACAGAGGGA AGGTTCCCTT CAGGTGGGCA ATGAGGCTCC CGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAGCTAGAC ACAGACGGAA CTCTATGGCT GGGTGGCCTG 
GAGAAGCTGC CCATAGGCCA GGCCCTGCCC AAGGCCTATG GCACGGGCTT TGTGGGCTGC 
CTGCGGGATG TGGTGGTGGG GCAGCGCCCA CTGCACCTGC TGGAGGATGC TGTCACCAAG 
CCAGAGCTCC GACCCTGTGC CGCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620361.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620418.1 Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA.

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CloneID	OCe06695
Clone ID Related Accession (Same CDS sequence)	XM_005620361.3 , XM_005620361.2
Accession Version	XM_005620361.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agrin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC 
CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC 
CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC 
TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC 
ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT 
GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT 
GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG 
GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG 
CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT 
GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG 
CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA 
GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC 
CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC 
CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA 
CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC 
ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA 
CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC 
ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC 
CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC 
CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG 
GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC 
CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG 
TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC 
TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG 
CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG 
TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC 
AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT 
GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC 
ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA 
GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC 
GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC 
CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG 
GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA 
GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC 
TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG 
GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC 
AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC 
TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG 
GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT 
GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGAAAT CCCGCAAGGT GCCACACACT 
GTCCTCAACC TGAAGGAGCC ACTCTATGTT GGAGGGGCCC CTGACTTCAG CAAACTGGCC 
CGGGCAGCTG CGGTATTCTC TGGCTTCGAT GGCGCCATCC AACTGGTCTC CTTGAAAGGC 
CACCAGCTGC TGTCCCAGGA GCACGTGGTG CGGTCAGTGG ATGTCTCATC TTTTGCCCAC 
CACCCTTGTA CTCAGGCCTC GGGCCACCCA TGCCTCAATG GGGCCTCCTG CCTCCCTCGA 
GAGGCCTCCT ATGAGTGCCT GTGCCCCGGG GGCTTCTCAG GGCTTCACTG TGAGAAGGGG 
CTGATTGAGA AGTCAGCAGG GGATGTGGAC GCCCTGGCCT TTGACGGACA GACCTACGTC 
GAGTACCTCA ATGCCGTGAC TGAGAGCGAA CTGACCAATG AGATCCCAGC TCCTGATGCT 
CCGGATTCCG GGGCCCTTCC CAGTGAGAAG GCCCTGCAGA GCAACCACTT TGAGCTGAGC 
CTGCGCACGG AAGCCACTCA GGGGCTGGTG CTGTGGAGCG GCAAGGCCAC GGAGCGGGCC 
GACTATGTCG CACTGGCCAT CGTGGATGGG CGCCTGCAGC TGACCTATGA CCTGGGCTCC 
CAGCCCGTCG TGCTGCGCTC CACCGTGCCG GTCAACACCA ACCGCTGGCT ACGAGTCAGG 
GCACATAGGG AACAGAGGGA AGGTTCCCTT CAGGTGGGCA ATGAGGCTCC CGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAGCTAGAC ACAGACGGAA CTCTATGGCT GGGTGGCCTG 
GAGAAGCTGC CCATAGGCCA GGCCCTGCCC AAGGCCTATG GCACGGGCTT TGTGGGCTGC 
CTGCGGGATG TGGTGGTGGG GCAGCGCCCA CTGCACCTGC TGGAGGATGC TGTCACCAAG 
CCAGAGCTCC GACCCTGTGC CGCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620418.1
CDS	133..6342
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAPDAPDSGALPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620361.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620361.2

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC 
CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC 
CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC 
TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC 
ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT 
GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT 
GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG 
GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG 
CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT 
GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG 
CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA 
GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC 
CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC 
CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA 
CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC 
ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA 
CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC 
ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC 
CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC 
CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG 
GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC 
CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG 
TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC 
TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG 
CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG 
TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC 
AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT 
GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC 
ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA 
GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC 
GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC 
CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG 
GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA 
GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC 
TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG 
GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC 
AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC 
TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG 
GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT 
GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGAAAT CCCGCAAGGT GCCACACACT 
GTCCTCAACC TGAAGGAGCC ACTCTATGTT GGAGGGGCCC CTGACTTCAG CAAACTGGCC 
CGGGCAGCTG CGGTATTCTC TGGCTTCGAT GGCGCCATCC AACTGGTCTC CTTGAAAGGC 
CACCAGCTGC TGTCCCAGGA GCACGTGGTG CGGTCAGTGG ATGTCTCATC TTTTGCCCAC 
CACCCTTGTA CTCAGGCCTC GGGCCACCCA TGCCTCAATG GGGCCTCCTG CCTCCCTCGA 
GAGGCCTCCT ATGAGTGCCT GTGCCCCGGG GGCTTCTCAG GGCTTCACTG TGAGAAGGGG 
CTGATTGAGA AGTCAGCAGG GGATGTGGAC GCCCTGGCCT TTGACGGACA GACCTACGTC 
GAGTACCTCA ATGCCGTGAC TGAGAGCGAA CTGACCAATG AGATCCCAGC TCCTGATGCT 
CCGGATTCCG GGGCCCTTCC CAGTGAGAAG GCCCTGCAGA GCAACCACTT TGAGCTGAGC 
CTGCGCACGG AAGCCACTCA GGGGCTGGTG CTGTGGAGCG GCAAGGCCAC GGAGCGGGCC 
GACTATGTCG CACTGGCCAT CGTGGATGGG CGCCTGCAGC TGACCTATGA CCTGGGCTCC 
CAGCCCGTCG TGCTGCGCTC CACCGTGCCG GTCAACACCA ACCGCTGGCT ACGAGTCAGG 
GCACATAGGG AACAGAGGGA AGGTTCCCTT CAGGTGGGCA ATGAGGCTCC CGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAGCTAGAC ACAGACGGAA CTCTATGGCT GGGTGGCCTG 
GAGAAGCTGC CCATAGGCCA GGCCCTGCCC AAGGCCTATG GCACGGGCTT TGTGGGCTGC 
CTGCGGGATG TGGTGGTGGG GCAGCGCCCA CTGCACCTGC TGGAGGATGC TGTCACCAAG 
CCAGAGCTCC GACCCTGTGC CGCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620362.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620419.1 Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA.

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CloneID	OCe06696
Clone ID Related Accession (Same CDS sequence)	XM_005620362.2 , XM_005620362.3
Accession Version	XM_005620362.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agrin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG 
ATCCCAGCTG AGAAGGCCCT GCAGAGCAAC CACTTTGAGC TGAGCCTGCG CACGGAAGCC 
ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACGGAGC GGGCCGACTA TGTCGCACTG 
GCCATCGTGG ATGGGCGCCT GCAGCTGACC TATGACCTGG GCTCCCAGCC CGTCGTGCTG 
CGCTCCACCG TGCCGGTCAA CACCAACCGC TGGCTACGAG TCAGGGCACA TAGGGAACAG 
AGGGAAGGTT CCCTTCAGGT GGGCAATGAG GCTCCCGTGA CTGGCTCTTC CCCGCTGGGT 
GCCACACAGC TAGACACAGA CGGAACTCTA TGGCTGGGTG GCCTGGAGAA GCTGCCCATA 
GGCCAGGCCC TGCCCAAGGC CTATGGCACG GGCTTTGTGG GCTGCCTGCG GGATGTGGTG 
GTGGGGCAGC GCCCACTGCA CCTGCTGGAG GATGCTGTCA CCAAGCCAGA GCTCCGACCC 
TGTGCCGCCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620419.1
CDS	133..6327
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620362.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620362.2

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG 
ATCCCAGCTG AGAAGGCCCT GCAGAGCAAC CACTTTGAGC TGAGCCTGCG CACGGAAGCC 
ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACGGAGC GGGCCGACTA TGTCGCACTG 
GCCATCGTGG ATGGGCGCCT GCAGCTGACC TATGACCTGG GCTCCCAGCC CGTCGTGCTG 
CGCTCCACCG TGCCGGTCAA CACCAACCGC TGGCTACGAG TCAGGGCACA TAGGGAACAG 
AGGGAAGGTT CCCTTCAGGT GGGCAATGAG GCTCCCGTGA CTGGCTCTTC CCCGCTGGGT 
GCCACACAGC TAGACACAGA CGGAACTCTA TGGCTGGGTG GCCTGGAGAA GCTGCCCATA 
GGCCAGGCCC TGCCCAAGGC CTATGGCACG GGCTTTGTGG GCTGCCTGCG GGATGTGGTG 
GTGGGGCAGC GCCCACTGCA CCTGCTGGAG GATGCTGTCA CCAAGCCAGA GCTCCGACCC 
TGTGCCGCCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620362.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620419.1 Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA.

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CloneID	OCe06696
Clone ID Related Accession (Same CDS sequence)	XM_005620362.2 , XM_005620362.3
Accession Version	XM_005620362.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG 
ATCCCAGCTG AGAAGGCCCT GCAGAGCAAC CACTTTGAGC TGAGCCTGCG CACGGAAGCC 
ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACGGAGC GGGCCGACTA TGTCGCACTG 
GCCATCGTGG ATGGGCGCCT GCAGCTGACC TATGACCTGG GCTCCCAGCC CGTCGTGCTG 
CGCTCCACCG TGCCGGTCAA CACCAACCGC TGGCTACGAG TCAGGGCACA TAGGGAACAG 
AGGGAAGGTT CCCTTCAGGT GGGCAATGAG GCTCCCGTGA CTGGCTCTTC CCCGCTGGGT 
GCCACACAGC TAGACACAGA CGGAACTCTA TGGCTGGGTG GCCTGGAGAA GCTGCCCATA 
GGCCAGGCCC TGCCCAAGGC CTATGGCACG GGCTTTGTGG GCTGCCTGCG GGATGTGGTG 
GTGGGGCAGC GCCCACTGCA CCTGCTGGAG GATGCTGTCA CCAAGCCAGA GCTCCGACCC 
TGTGCCGCCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620419.1
CDS	81..6275
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESELTNEIPAEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620362.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620362.3

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGCGAACT GACCAATGAG 
ATCCCAGCTG AGAAGGCCCT GCAGAGCAAC CACTTTGAGC TGAGCCTGCG CACGGAAGCC 
ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACGGAGC GGGCCGACTA TGTCGCACTG 
GCCATCGTGG ATGGGCGCCT GCAGCTGACC TATGACCTGG GCTCCCAGCC CGTCGTGCTG 
CGCTCCACCG TGCCGGTCAA CACCAACCGC TGGCTACGAG TCAGGGCACA TAGGGAACAG 
AGGGAAGGTT CCCTTCAGGT GGGCAATGAG GCTCCCGTGA CTGGCTCTTC CCCGCTGGGT 
GCCACACAGC TAGACACAGA CGGAACTCTA TGGCTGGGTG GCCTGGAGAA GCTGCCCATA 
GGCCAGGCCC TGCCCAAGGC CTATGGCACG GGCTTTGTGG GCTGCCTGCG GGATGTGGTG 
GTGGGGCAGC GCCCACTGCA CCTGCTGGAG GATGCTGTCA CCAAGCCAGA GCTCCGACCC 
TGTGCCGCCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620363.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620420.1 Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA.

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CloneID	OCe06697
Clone ID Related Accession (Same CDS sequence)	XM_005620363.2 , XM_005620363.1
Accession Version	XM_005620363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agrin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGTGAGAA GGCCCTGCAG 
AGCAACCACT TTGAGCTGAG CCTGCGCACG GAAGCCACTC AGGGGCTGGT GCTGTGGAGC 
GGCAAGGCCA CGGAGCGGGC CGACTATGTC GCACTGGCCA TCGTGGATGG GCGCCTGCAG 
CTGACCTATG ACCTGGGCTC CCAGCCCGTC GTGCTGCGCT CCACCGTGCC GGTCAACACC 
AACCGCTGGC TACGAGTCAG GGCACATAGG GAACAGAGGG AAGGTTCCCT TCAGGTGGGC 
AATGAGGCTC CCGTGACTGG CTCTTCCCCG CTGGGTGCCA CACAGCTAGA CACAGACGGA 
ACTCTATGGC TGGGTGGCCT GGAGAAGCTG CCCATAGGCC AGGCCCTGCC CAAGGCCTAT 
GGCACGGGCT TTGTGGGCTG CCTGCGGGAT GTGGTGGTGG GGCAGCGCCC ACTGCACCTG 
CTGGAGGATG CTGTCACCAA GCCAGAGCTC CGACCCTGTG CCGCCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620420.1
CDS	80..6250
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620363.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620363.2

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGTGAGAA GGCCCTGCAG 
AGCAACCACT TTGAGCTGAG CCTGCGCACG GAAGCCACTC AGGGGCTGGT GCTGTGGAGC 
GGCAAGGCCA CGGAGCGGGC CGACTATGTC GCACTGGCCA TCGTGGATGG GCGCCTGCAG 
CTGACCTATG ACCTGGGCTC CCAGCCCGTC GTGCTGCGCT CCACCGTGCC GGTCAACACC 
AACCGCTGGC TACGAGTCAG GGCACATAGG GAACAGAGGG AAGGTTCCCT TCAGGTGGGC 
AATGAGGCTC CCGTGACTGG CTCTTCCCCG CTGGGTGCCA CACAGCTAGA CACAGACGGA 
ACTCTATGGC TGGGTGGCCT GGAGAAGCTG CCCATAGGCC AGGCCCTGCC CAAGGCCTAT 
GGCACGGGCT TTGTGGGCTG CCTGCGGGAT GTGGTGGTGG GGCAGCGCCC ACTGCACCTG 
CTGGAGGATG CTGTCACCAA GCCAGAGCTC CGACCCTGTG CCGCCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620363.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620420.1 Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA.

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CloneID	OCe06697
Clone ID Related Accession (Same CDS sequence)	XM_005620363.2 , XM_005620363.1
Accession Version	XM_005620363.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agrin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGTGAGAA GGCCCTGCAG 
AGCAACCACT TTGAGCTGAG CCTGCGCACG GAAGCCACTC AGGGGCTGGT GCTGTGGAGC 
GGCAAGGCCA CGGAGCGGGC CGACTATGTC GCACTGGCCA TCGTGGATGG GCGCCTGCAG 
CTGACCTATG ACCTGGGCTC CCAGCCCGTC GTGCTGCGCT CCACCGTGCC GGTCAACACC 
AACCGCTGGC TACGAGTCAG GGCACATAGG GAACAGAGGG AAGGTTCCCT TCAGGTGGGC 
AATGAGGCTC CCGTGACTGG CTCTTCCCCG CTGGGTGCCA CACAGCTAGA CACAGACGGA 
ACTCTATGGC TGGGTGGCCT GGAGAAGCTG CCCATAGGCC AGGCCCTGCC CAAGGCCTAT 
GGCACGGGCT TTGTGGGCTG CCTGCGGGAT GTGGTGGTGG GGCAGCGCCC ACTGCACCTG 
CTGGAGGATG CTGTCACCAA GCCAGAGCTC CGACCCTGTG CCGCCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620420.1
CDS	132..6302
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620363.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620363.1

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGAAATCC 
CGCAAGGTGC CACACACTGT CCTCAACCTG AAGGAGCCAC TCTATGTTGG AGGGGCCCCT 
GACTTCAGCA AACTGGCCCG GGCAGCTGCG GTATTCTCTG GCTTCGATGG CGCCATCCAA 
CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT 
GTCTCATCTT TTGCCCACCA CCCTTGTACT CAGGCCTCGG GCCACCCATG CCTCAATGGG 
GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG 
CTTCACTGTG AGAAGGGGCT GATTGAGAAG TCAGCAGGGG ATGTGGACGC CCTGGCCTTT 
GACGGACAGA CCTACGTCGA GTACCTCAAT GCCGTGACTG AGAGTGAGAA GGCCCTGCAG 
AGCAACCACT TTGAGCTGAG CCTGCGCACG GAAGCCACTC AGGGGCTGGT GCTGTGGAGC 
GGCAAGGCCA CGGAGCGGGC CGACTATGTC GCACTGGCCA TCGTGGATGG GCGCCTGCAG 
CTGACCTATG ACCTGGGCTC CCAGCCCGTC GTGCTGCGCT CCACCGTGCC GGTCAACACC 
AACCGCTGGC TACGAGTCAG GGCACATAGG GAACAGAGGG AAGGTTCCCT TCAGGTGGGC 
AATGAGGCTC CCGTGACTGG CTCTTCCCCG CTGGGTGCCA CACAGCTAGA CACAGACGGA 
ACTCTATGGC TGGGTGGCCT GGAGAAGCTG CCCATAGGCC AGGCCCTGCC CAAGGCCTAT 
GGCACGGGCT TTGTGGGCTG CCTGCGGGAT GTGGTGGTGG GGCAGCGCCC ACTGCACCTG 
CTGGAGGATG CTGTCACCAA GCCAGAGCTC CGACCCTGTG CCGCCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620364.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620421.1 Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA.

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CloneID	OCe06698
Clone ID Related Accession (Same CDS sequence)	XM_005620364.1 , XM_005620364.2
Accession Version	XM_005620364.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agrin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA 
CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA 
CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG 
AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT 
GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC 
CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG 
AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC 
TACGTCGAGT ACCTCAATGC CGTGACTGAG AGTGAGAAGG CCCTGCAGAG CAACCACTTT 
GAGCTGAGCC TGCGCACGGA AGCCACTCAG GGGCTGGTGC TGTGGAGCGG CAAGGCCACG 
GAGCGGGCCG ACTATGTCGC ACTGGCCATC GTGGATGGGC GCCTGCAGCT GACCTATGAC 
CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC ACCGTGCCGG TCAACACCAA CCGCTGGCTA 
CGAGTCAGGG CACATAGGGA ACAGAGGGAA GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC 
GTGACTGGCT CTTCCCCGCT GGGTGCCACA CAGCTAGACA CAGACGGAAC TCTATGGCTG 
GGTGGCCTGG AGAAGCTGCC CATAGGCCAG GCCCTGCCCA AGGCCTATGG CACGGGCTTT 
GTGGGCTGCC TGCGGGATGT GGTGGTGGGG CAGCGCCCAC TGCACCTGCT GGAGGATGCT 
GTCACCAAGC CAGAGCTCCG ACCCTGTGCC GCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620421.1
CDS	132..6290
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620364.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620364.1

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA 
CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA 
CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG 
AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT 
GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC 
CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG 
AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC 
TACGTCGAGT ACCTCAATGC CGTGACTGAG AGTGAGAAGG CCCTGCAGAG CAACCACTTT 
GAGCTGAGCC TGCGCACGGA AGCCACTCAG GGGCTGGTGC TGTGGAGCGG CAAGGCCACG 
GAGCGGGCCG ACTATGTCGC ACTGGCCATC GTGGATGGGC GCCTGCAGCT GACCTATGAC 
CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC ACCGTGCCGG TCAACACCAA CCGCTGGCTA 
CGAGTCAGGG CACATAGGGA ACAGAGGGAA GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC 
GTGACTGGCT CTTCCCCGCT GGGTGCCACA CAGCTAGACA CAGACGGAAC TCTATGGCTG 
GGTGGCCTGG AGAAGCTGCC CATAGGCCAG GCCCTGCCCA AGGCCTATGG CACGGGCTTT 
GTGGGCTGCC TGCGGGATGT GGTGGTGGGG CAGCGCCCAC TGCACCTGCT GGAGGATGCT 
GTCACCAAGC CAGAGCTCCG ACCCTGTGCC GCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005620364.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620421.1 Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA.

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CloneID	OCe06698
Clone ID Related Accession (Same CDS sequence)	XM_005620364.1 , XM_005620364.2
Accession Version	XM_005620364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agrin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA 
CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA 
CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG 
AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT 
GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC 
CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG 
AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC 
TACGTCGAGT ACCTCAATGC CGTGACTGAG AGTGAGAAGG CCCTGCAGAG CAACCACTTT 
GAGCTGAGCC TGCGCACGGA AGCCACTCAG GGGCTGGTGC TGTGGAGCGG CAAGGCCACG 
GAGCGGGCCG ACTATGTCGC ACTGGCCATC GTGGATGGGC GCCTGCAGCT GACCTATGAC 
CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC ACCGTGCCGG TCAACACCAA CCGCTGGCTA 
CGAGTCAGGG CACATAGGGA ACAGAGGGAA GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC 
GTGACTGGCT CTTCCCCGCT GGGTGCCACA CAGCTAGACA CAGACGGAAC TCTATGGCTG 
GGTGGCCTGG AGAAGCTGCC CATAGGCCAG GCCCTGCCCA AGGCCTATGG CACGGGCTTT 
GTGGGCTGCC TGCGGGATGT GGTGGTGGGG CAGCGCCCAC TGCACCTGCT GGAGGATGCT 
GTCACCAAGC CAGAGCTCCG ACCCTGTGCC GCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620421.1
CDS	80..6238
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQDSVLLAETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_005620364.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620364.2

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGACT CTGTTCTCCT TGCAGAGACT 
GTTGCTCCTG GGGCCCGCCC AACATCTGTA CCCAGGGCCA CCTCAGGGCT CAGCACGAGC 
AAGGCGCTGC CACCCGCCCC CAGCACCCTC CCCCTGGCTC CCAGCAGCAC CCCTCATAGC 
CGGCCCACGC CTCGACCCTC ACCAGGATCT CGGACCACTG CCAGCATTCC CAGGACTGTC 
ACGAGGCCTG TGCTGACCAT GCCCCCCACA GCCCCCGCCA CGGCAGCCAA CCTCGCCACA 
TCTGCTTTTG GCGAGTCTGG CAGTGCTGAC GGGAGCGGCG ATGAGGGGCT GAGTGGTGAC 
CTGGAGGCCA GTGGGACTGG CTCAGGGGGA CTCGAGCCCC CTGAAGGTGG CAGTGCTGTG 
ACCCCTGAGC CACCCATGGA GAGGGCTTCT TGCTACAACT CGCCTTTGGG CTGCTGCTCG 
GATGGCAAGA CGCCCTCGCT GGACGCAGAG GGTTCCAACT GTCCTGCCAC CAAGGCGTTC 
CAGGGTGTGT TGGAGCTTGA GGGCGTGGAG GGGCAAGAGC TGTTCTACAC GCCGGAGATG 
GCTGACCCCA AGTCAGAGCT TTTTGGGGAG ACTGCTAGGA GCATCGAGAG CACCCTGGAT 
GACCTCTTCC GGAACTCAGA GGTTAAGAAG GACTTCCGAA GCGTCCGGCT GAGGGACTTG 
GGGCCTGGCA GCTCAGTCCG AGCCATTGTG GATGTGCACT TTGACCCTGC CACAACCTTT 
AGGGCGCCTG ATGTGGGCCA AGCCCTGCTC CGGCAGATAC AGGCATCCCG GCGCCGGTCC 
CTGGGCGTAC GGCGGCCACT GCAGGAGCAC GTGCGCTTTA TGGACTTTGA CTGGTTTCCT 
GCGTTCTTCA CGGGAGCCAC CACAGGAGCA ACACCCGCCG CAGCCACGGC CAGAGCCACC 
ACGGTGGCCC ACCTGCCACC TGCTGGGACC CAGTGGGCCC TCTACCCCAG TCACACAAGC 
CGGCCTGTGG GCAGGACCAC AGCTCCACCC ACCACACGCC AGCCTCCCAC CACTGCTCCC 
CGTCGCGTGC CCGGACGCCG GCCCTTGCCC CCAGGCACCC AGCAGCCCCC GAAGCCCTGC 
GACTCCCAGC CCTGCCTCCA TGGGGGAACC TGCCAGGACC AGGACTCGGG TGGAGGCTTC 
ACCTGCAGCT GCCCAGTGGG CAGGAGGGGT GCCGTCTGTG AGAAGGCCCT GTACCTCTCG 
GTGCCAGCTT TCAATGGCCG CTCCTTCCTG GCCTTCCCCA CCCTCCGTGC CTACCACACG 
CTGCGCCTAG CACTTGAGTT CCGGGCACTA GAGCCACAGG GCCTGCTGCT CTATAATGGC 
AATGCCCAGG GCAAGGACTT CCTGGCATTG ACGCTGCTGG GGGGCCGTGT GCAGCTCAGG 
TTTGACACAG GTTCCGGGCC TGCAGTACTG ACCAGCTCAG TGCCTATAGA GCCTGGCCGC 
TGGCATCGCT TGGAGCTGTC TCGGCACTGG CGCCGGGGAA CACTTTCAGT GGATGGTGAG 
CCCCTGGTGC TGGGCCACAG CCCCAGCGGT ACAGATGGTC TCAACCTGGA CACAGACCTT 
TTTGTGGGCG GCATCCCTGA GGATCAGGCT TCTGTGGTAC TCGAAAGGAC GTCCATCAGC 
GTTGGCCTCA AGGGCTGCAT CCGCCTGCTG GATGTCAATA ATCAGCGTTT AGAGCTCAGC 
AGCTGGCCAG GGGCTGCAGC CCGAAGCTCC GGGGTGGGCG AGTGTGGAGA CCACCCCTGC 
CTGCCCAACC CCTGCCTCGA CGGGGCTCCG TGCCAGGCCC TGGAGGCTGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCCA ACCTGTAGTG GTGACAAGAA CCCCTGTGAG 
CCGAACCCCT GCCATGGGGC AGCCCCCTGC CGTGTGCTGC CTGATGGGGA GGCCAAGTGT 
GAATGCCCCC GAGGACGAGG GGGTGCCATC TGCCAGACAG TCTCGGAGCG GGATTCTTCC 
CAGCCCTTCC TGGCTGACTT CCATGGCTTC TCCTACCTGG AGCTGAAAGG GTTGCATACC 
TTTGAGCGGG ACCTGGGGGA GAAGCTGGCA CTGGAGGTGG TGTTCCTGGC CCGTGGGCCC 
AGTGGTTTGC TCCTCTACAA TGGGCAGAAA ACAGACGGCA AGGGGGACTT TGTGTCACTG 
GCGTTGCATG ACCGCTTCTT GGAATTCCGC TATGACCTGG GCAAGGGAGC AGCGGTCATC 
AGGAGCAAGG AGCCGGTGGC CCTGGACACC TGGACCAGGG TCTCACTAGA GCGCAATGGC 
CGCAAGGGTG CCATGCGTGT CAGTGATGGG CCTCGCGTGC TGGGAGAGTC CCCGGTGCCA 
CACACTGTCC TCAACCTGAA GGAGCCACTC TATGTTGGAG GGGCCCCTGA CTTCAGCAAA 
CTGGCCCGGG CAGCTGCGGT ATTCTCTGGC TTCGATGGCG CCATCCAACT GGTCTCCTTG 
AAAGGCCACC AGCTGCTGTC CCAGGAGCAC GTGGTGCGGT CAGTGGATGT CTCATCTTTT 
GCCCACCACC CTTGTACTCA GGCCTCGGGC CACCCATGCC TCAATGGGGC CTCCTGCCTC 
CCTCGAGAGG CCTCCTATGA GTGCCTGTGC CCCGGGGGCT TCTCAGGGCT TCACTGTGAG 
AAGGGGCTGA TTGAGAAGTC AGCAGGGGAT GTGGACGCCC TGGCCTTTGA CGGACAGACC 
TACGTCGAGT ACCTCAATGC CGTGACTGAG AGTGAGAAGG CCCTGCAGAG CAACCACTTT 
GAGCTGAGCC TGCGCACGGA AGCCACTCAG GGGCTGGTGC TGTGGAGCGG CAAGGCCACG 
GAGCGGGCCG ACTATGTCGC ACTGGCCATC GTGGATGGGC GCCTGCAGCT GACCTATGAC 
CTGGGCTCCC AGCCCGTCGT GCTGCGCTCC ACCGTGCCGG TCAACACCAA CCGCTGGCTA 
CGAGTCAGGG CACATAGGGA ACAGAGGGAA GGTTCCCTTC AGGTGGGCAA TGAGGCTCCC 
GTGACTGGCT CTTCCCCGCT GGGTGCCACA CAGCTAGACA CAGACGGAAC TCTATGGCTG 
GGTGGCCTGG AGAAGCTGCC CATAGGCCAG GCCCTGCCCA AGGCCTATGG CACGGGCTTT 
GTGGGCTGCC TGCGGGATGT GGTGGTGGGG CAGCGCCCAC TGCACCTGCT GGAGGATGCT 
GTCACCAAGC CAGAGCTCCG ACCCTGTGCC GCCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_536713.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_536713.3 Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA.

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CloneID	OCe06699
Clone ID Related Accession (Same CDS sequence)	XM_536713.6 , XM_536713.5
Accession Version	XM_536713.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agrin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_536713.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC 
CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC 
CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC 
TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC 
ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT 
GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT 
GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG 
GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG 
CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT 
GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG 
CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA 
GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC 
CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC 
CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA 
CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC 
ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA 
CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC 
ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC 
CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC 
CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG 
GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC 
CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG 
TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC 
TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG 
CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG 
TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC 
AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT 
GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC 
ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA 
GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC 
GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC 
CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG 
GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA 
GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC 
TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG 
GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC 
AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC 
TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG 
GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT 
GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGGTGC CACACACTGT CCTCAACCTG 
AAGGAGCCAC TCTATGTTGG AGGGGCCCCT GACTTCAGCA AACTGGCCCG GGCAGCTGCG 
GTATTCTCTG GCTTCGATGG CGCCATCCAA CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG 
TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT GTCTCATCTT TTGCCCACCA CCCTTGTACT 
CAGGCCTCGG GCCACCCATG CCTCAATGGG GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT 
GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG CTTCACTGTG AGAAGGGGCT GATTGAGAAG 
TCAGCAGGGG ATGTGGACGC CCTGGCCTTT GACGGACAGA CCTACGTCGA GTACCTCAAT 
GCCGTGACTG AGAGTGAGAA GGCCCTGCAG AGCAACCACT TTGAGCTGAG CCTGCGCACG 
GAAGCCACTC AGGGGCTGGT GCTGTGGAGC GGCAAGGCCA CGGAGCGGGC CGACTATGTC 
GCACTGGCCA TCGTGGATGG GCGCCTGCAG CTGACCTATG ACCTGGGCTC CCAGCCCGTC 
GTGCTGCGCT CCACCGTGCC GGTCAACACC AACCGCTGGC TACGAGTCAG GGCACATAGG 
GAACAGAGGG AAGGTTCCCT TCAGGTGGGC AATGAGGCTC CCGTGACTGG CTCTTCCCCG 
CTGGGTGCCA CACAGCTAGA CACAGACGGA ACTCTATGGC TGGGTGGCCT GGAGAAGCTG 
CCCATAGGCC AGGCCCTGCC CAAGGCCTAT GGCACGGGCT TTGTGGGCTG CCTGCGGGAT 
GTGGTGGTGG GGCAGCGCCC ACTGCACCTG CTGGAGGATG CTGTCACCAA GCCAGAGCTC 
CGACCCTGTG CCGCCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536713.3
CDS	1..6141
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_536713.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536713.6

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC 
CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC 
CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC 
TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC 
ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT 
GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT 
GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG 
GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG 
CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT 
GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG 
CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA 
GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC 
CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC 
CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA 
CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC 
ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA 
CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC 
ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC 
CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC 
CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG 
GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC 
CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG 
TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC 
TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG 
CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG 
TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC 
AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT 
GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC 
ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA 
GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC 
GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC 
CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG 
GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA 
GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC 
TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG 
GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC 
AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC 
TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG 
GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT 
GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGGTGC CACACACTGT CCTCAACCTG 
AAGGAGCCAC TCTATGTTGG AGGGGCCCCT GACTTCAGCA AACTGGCCCG GGCAGCTGCG 
GTATTCTCTG GCTTCGATGG CGCCATCCAA CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG 
TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT GTCTCATCTT TTGCCCACCA CCCTTGTACT 
CAGGCCTCGG GCCACCCATG CCTCAATGGG GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT 
GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG CTTCACTGTG AGAAGGGGCT GATTGAGAAG 
TCAGCAGGGG ATGTGGACGC CCTGGCCTTT GACGGACAGA CCTACGTCGA GTACCTCAAT 
GCCGTGACTG AGAGTGAGAA GGCCCTGCAG AGCAACCACT TTGAGCTGAG CCTGCGCACG 
GAAGCCACTC AGGGGCTGGT GCTGTGGAGC GGCAAGGCCA CGGAGCGGGC CGACTATGTC 
GCACTGGCCA TCGTGGATGG GCGCCTGCAG CTGACCTATG ACCTGGGCTC CCAGCCCGTC 
GTGCTGCGCT CCACCGTGCC GGTCAACACC AACCGCTGGC TACGAGTCAG GGCACATAGG 
GAACAGAGGG AAGGTTCCCT TCAGGTGGGC AATGAGGCTC CCGTGACTGG CTCTTCCCCG 
CTGGGTGCCA CACAGCTAGA CACAGACGGA ACTCTATGGC TGGGTGGCCT GGAGAAGCTG 
CCCATAGGCC AGGCCCTGCC CAAGGCCTAT GGCACGGGCT TTGTGGGCTG CCTGCGGGAT 
GTGGTGGTGG GGCAGCGCCC ACTGCACCTG CTGGAGGATG CTGTCACCAA GCCAGAGCTC 
CGACCCTGTG CCGCCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_536713.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_536713.3 Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA.

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CloneID	OCe06699
Clone ID Related Accession (Same CDS sequence)	XM_536713.6 , XM_536713.5
Accession Version	XM_536713.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agrin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_536713.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC 
CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC 
CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC 
TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC 
ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT 
GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT 
GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG 
GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG 
CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT 
GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG 
CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA 
GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC 
CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC 
CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA 
CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC 
ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA 
CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC 
ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC 
CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC 
CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG 
GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC 
CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG 
TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC 
TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG 
CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG 
TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC 
AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT 
GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC 
ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA 
GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC 
GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC 
CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG 
GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA 
GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC 
TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG 
GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC 
AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC 
TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG 
GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT 
GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGGTGC CACACACTGT CCTCAACCTG 
AAGGAGCCAC TCTATGTTGG AGGGGCCCCT GACTTCAGCA AACTGGCCCG GGCAGCTGCG 
GTATTCTCTG GCTTCGATGG CGCCATCCAA CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG 
TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT GTCTCATCTT TTGCCCACCA CCCTTGTACT 
CAGGCCTCGG GCCACCCATG CCTCAATGGG GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT 
GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG CTTCACTGTG AGAAGGGGCT GATTGAGAAG 
TCAGCAGGGG ATGTGGACGC CCTGGCCTTT GACGGACAGA CCTACGTCGA GTACCTCAAT 
GCCGTGACTG AGAGTGAGAA GGCCCTGCAG AGCAACCACT TTGAGCTGAG CCTGCGCACG 
GAAGCCACTC AGGGGCTGGT GCTGTGGAGC GGCAAGGCCA CGGAGCGGGC CGACTATGTC 
GCACTGGCCA TCGTGGATGG GCGCCTGCAG CTGACCTATG ACCTGGGCTC CCAGCCCGTC 
GTGCTGCGCT CCACCGTGCC GGTCAACACC AACCGCTGGC TACGAGTCAG GGCACATAGG 
GAACAGAGGG AAGGTTCCCT TCAGGTGGGC AATGAGGCTC CCGTGACTGG CTCTTCCCCG 
CTGGGTGCCA CACAGCTAGA CACAGACGGA ACTCTATGGC TGGGTGGCCT GGAGAAGCTG 
CCCATAGGCC AGGCCCTGCC CAAGGCCTAT GGCACGGGCT TTGTGGGCTG CCTGCGGGAT 
GTGGTGGTGG GGCAGCGCCC ACTGCACCTG CTGGAGGATG CTGTCACCAA GCCAGAGCTC 
CGACCCTGTG CCGCCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536713.3
CDS	132..6272
Translation	MASLRRSGPAPLQLLLLLLVVAARALTSADGTCPERALEKGEEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDMVTQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLACTRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPESCPSQPAPVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVVSAGHCQTCGDTVCAFGAVCLAGQCVCPRCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSYSGTCDPTTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHALGPGGCETDSLAPETCAEMHCEFGASCVEEAGSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQETVAPGARPTSVPRATSGLSTSKALPPAPSTLPLAPSSTPHSRPTPRPSPGSRTTASIPRTVTRPVLTMPPTAPATAANLATSAFGESGSADGSGDEGLSGDLEASGTGSGGLEPPEGGSAVTPEPPMERASCYNSPLGCCSDGKTPSLDAEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSEVKKDFRSVRLRDLGPGSSVRAIVDVHFDPATTFRAPDVGQALLRQIQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATTGATPAAATARATTVAHLPPAGTQWALYPSHTSRPVGRTTAPPTTRQPPTTAPRRVPGRRPLPPGTQQPPKPCDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLSVPAFNGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNAQGKDFLALTLLGGRVQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGIPEDQASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPGAAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNPCEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQTVSERDSSQPFLADFHGFSYLELKGLHTFERDLGEKLALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDRFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVFSGFDGAIQLVSLKGHQLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGRLQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLEKLPIGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDAVTKPELRPCAAP

Target ORF information:

RefSeq Version	XM_536713.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agrin (AGRN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536713.5

ORF Insert Sequence:

ATGGCCAGCC TGCGGCGGTC CGGCCCGGCC CCGCTGCAGC TGCTCCTGCT GCTCCTGGTG 
GTGGCTGCGC GCGCTCTAAC CAGCGCCGAC GGGACGTGCC CGGAACGCGC GCTGGAGAAG 
GGGGAGGAGG AGGCGAACGT GGTGCTCACC GGCACTGTGG AGGAGATTCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC GTGCAAGGTT CGAGTCTGGC GGTACTTGAA GGGCAAAGAC 
ATGGTGACCC AAGAGAGTCT GCTTGATGGT GGCAACAAGG TGGTGATTGG TGGCTTTGGA 
GATCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGGGATA CCAGAATTTT CTTTGTGAAC 
CCTGCACCGC CATACCTGTG GCCAGCCCAT AAGAATGAGC TGATGCTCAA TTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA TCTGGAGGAA GTAGAGCACT GTGTAGAAGA CAAACCTGGG 
ACCCACTTCA CACCAGCGCC CCCAACGCCT CCAGATGCAT GCCGAGGAAT GCTGTGTGGC 
TTCGGTGCTG TGTGTGAGCC CAGTGCAGGG GGTTCAGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGTGCCT GTCCCGCCGT GGTGGCGCCC GTGTGTGGCT CTGACGCCTC CACCTACAGC 
AATGAGTGTG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGC GCCGCATCCG CCTACTCAGT 
CGCGGACCCT GTGGTTCCCG GGACCCCTGC TCCAATGTGA CATGCAGCTT CGGCAGCACC 
TGTGTGCGCT CAGCAGATGG GCAGACAGCC ACATGCCTGT GCCCTGCCAC CTGCCGGGGG 
CCCCCGGAGG GCCCCGTGTG TGGGAGCGAC GGTGCTGACT ACCCCAGTGA GTGCCAGCTC 
CTGCGCCTCG CGTGCACTCG CCAGGAGAAC ATCGTCAAGA AGTTTGACGG CCCTTGTGAC 
CCCTGCCAGG GCACCCACCA TGACCTGAGC CGTGTCTGCC GCGTTCACCC ACGCACGCGG 
CATCCTGAGA TGCTCCTCCG GCCAGAGAGT TGCCCTTCTC AGCCAGCACC TGTGTGCGGG 
GATGATGGAG TCACCTATGA CAACGACTGC ATAATGGGCC GCACGGGAGC CACCCGTGGC 
CTTGTCCTCC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG TCCAGATGTG 
TGCAGGTTCA ATGCTGTGTG TCTGTCCCGC CGAGGCCGCC CCCGCTGCTC CTGTGACCGT 
GTCATCTGTG ATGGGGCTTA TAGACCCGTG TGTGCTCACG ACGGGCACAC GTATGATAAT 
GACTGCTGGC GTCAGCAAGC TGAGTGTCGG CAGCAGCGCA CCATTCCCGC CAAGCACCAG 
GGCCCGTGTG ACCAGCCCCT GTCCCCGTGC CTTGGGGTGC GGTGTCCTTT TGGGGCAACA 
TGTGCCGTGA AGAATGGGGA GGCTGAGTGC GTGTGCCAGC AGGTGTGTTC GGGCATCTAT 
GATCCTGTGT GTGGCAGTGA TGGTGTCACA TATGGCAGCA CATGTGAACT GGAAGCCACG 
GCCTGCGCCC TTGGACGGGA GATCCGGGTG GCTCGCAGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCTCT GTGTGAGGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAG 
TGCGTGGCCT CGGCTCAACC TGTGTGCGGT TCTGATGGGC ACACGTATGC CAGCGAGTGT 
GAGCTGCACG TCCACGCCTG CACACACCAG ATCAGCCTGC ATGTGGTCTC AGCTGGACAC 
TGCCAGACCT GCGGAGACAC AGTGTGTGCT TTCGGGGCAG TGTGCTTGGC TGGGCAATGT 
GTGTGTCCCC GATGTGAGCG ACCCCCACCC GGGCCCGTGT GTGGCAGCGA TGGTGTCACC 
TACGACAGTG CCTGTCATCT CCGAGAAGCT GCCTGCCAGC AACAGACACA GATTGAGGAG 
GCCCGGGCGG GGCCCTGTGA GCAGGCTGAG TGTGGCTCTG GAGGCTCTGG CTCCGGAGAG 
GATGGCGAGT GTGAGCAGGA GCTGTGCCAG CAGCATGGCG GTGTCTGGGA TGAGGACTCG 
GAAGATGGGC CTTGCGTCTG TGACTTCAGC TGCCATAGCG TCCTGAGGAG CCCGGTCTGT 
GGCTCAGATG GGCTCACCTA TGGCACCGAG TGTGAGCTGA AGAAGGCCAG GTGTGAATCG 
CGGCGAGAAC TGTACGTCAC AGCACAGGGA GCCTGCCGTG GCCCCACCTT GGCCCCATTG 
CTACCTGCAG TTCCCCCACA CTGCACCCAG ACCCCCTATG GCTGCTGCCA GGACAACGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CCTGCCAATG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAACT ACGGGTCAGT GCTCTTGCCG CCCAGGCGTC 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACT 
GATGGCCGGA GCGGCTGCAC CCCCTGCAGC TGTGACCCCC GGGGCGCTGT GCGGGATGAC 
TGCGAGCAGA TGACCGGGCT GTGTTCATGT AAGCCTGGGG TGGCTGGACC TAAGTGCGGG 
CAGTGTCCAG ATGGCCACGC TCTGGGCCCT GGCGGCTGTG AAACAGACTC CTTGGCGCCT 
GAGACGTGTG CTGAGATGCA CTGTGAGTTT GGGGCGTCCT GCGTGGAGGA GGCTGGCTCT 
GCACGCTGTG TGTGCCCAAC ATCTACCTGT CCAGGGGCCA GTGCTACCAA GGTCTGTGGG 
TCAGATGGAG TCACCTACGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGACATCT CCATCCAGAG CCTTGGCCCG TGCCAGGAGA CTGTTGCTCC TGGGGCCCGC 
CCAACATCTG TACCCAGGGC CACCTCAGGG CTCAGCACGA GCAAGGCGCT GCCACCCGCC 
CCCAGCACCC TCCCCCTGGC TCCCAGCAGC ACCCCTCATA GCCGGCCCAC GCCTCGACCC 
TCACCAGGAT CTCGGACCAC TGCCAGCATT CCCAGGACTG TCACGAGGCC TGTGCTGACC 
ATGCCCCCCA CAGCCCCCGC CACGGCAGCC AACCTCGCCA CATCTGCTTT TGGCGAGTCT 
GGCAGTGCTG ACGGGAGCGG CGATGAGGGG CTGAGTGGTG ACCTGGAGGC CAGTGGGACT 
GGCTCAGGGG GACTCGAGCC CCCTGAAGGT GGCAGTGCTG TGACCCCTGA GCCACCCATG 
GAGAGGGCTT CTTGCTACAA CTCGCCTTTG GGCTGCTGCT CGGATGGCAA GACGCCCTCG 
CTGGACGCAG AGGGTTCCAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GTTGGAGCTT 
GAGGGCGTGG AGGGGCAAGA GCTGTTCTAC ACGCCGGAGA TGGCTGACCC CAAGTCAGAG 
CTTTTTGGGG AGACTGCTAG GAGCATCGAG AGCACCCTGG ATGACCTCTT CCGGAACTCA 
GAGGTTAAGA AGGACTTCCG AAGCGTCCGG CTGAGGGACT TGGGGCCTGG CAGCTCAGTC 
CGAGCCATTG TGGATGTGCA CTTTGACCCT GCCACAACCT TTAGGGCGCC TGATGTGGGC 
CAAGCCCTGC TCCGGCAGAT ACAGGCATCC CGGCGCCGGT CCCTGGGCGT ACGGCGGCCA 
CTGCAGGAGC ACGTGCGCTT TATGGACTTT GACTGGTTTC CTGCGTTCTT CACGGGAGCC 
ACCACAGGAG CAACACCCGC CGCAGCCACG GCCAGAGCCA CCACGGTGGC CCACCTGCCA 
CCTGCTGGGA CCCAGTGGGC CCTCTACCCC AGTCACACAA GCCGGCCTGT GGGCAGGACC 
ACAGCTCCAC CCACCACACG CCAGCCTCCC ACCACTGCTC CCCGTCGCGT GCCCGGACGC 
CGGCCCTTGC CCCCAGGCAC CCAGCAGCCC CCGAAGCCCT GCGACTCCCA GCCCTGCCTC 
CATGGGGGAA CCTGCCAGGA CCAGGACTCG GGTGGAGGCT TCACCTGCAG CTGCCCAGTG 
GGCAGGAGGG GTGCCGTCTG TGAGAAGGCC CTGTACCTCT CGGTGCCAGC TTTCAATGGC 
CGCTCCTTCC TGGCCTTCCC CACCCTCCGT GCCTACCACA CGCTGCGCCT AGCACTTGAG 
TTCCGGGCAC TAGAGCCACA GGGCCTGCTG CTCTATAATG GCAATGCCCA GGGCAAGGAC 
TTCCTGGCAT TGACGCTGCT GGGGGGCCGT GTGCAGCTCA GGTTTGACAC AGGTTCCGGG 
CCTGCAGTAC TGACCAGCTC AGTGCCTATA GAGCCTGGCC GCTGGCATCG CTTGGAGCTG 
TCTCGGCACT GGCGCCGGGG AACACTTTCA GTGGATGGTG AGCCCCTGGT GCTGGGCCAC 
AGCCCCAGCG GTACAGATGG TCTCAACCTG GACACAGACC TTTTTGTGGG CGGCATCCCT 
GAGGATCAGG CTTCTGTGGT ACTCGAAAGG ACGTCCATCA GCGTTGGCCT CAAGGGCTGC 
ATCCGCCTGC TGGATGTCAA TAATCAGCGT TTAGAGCTCA GCAGCTGGCC AGGGGCTGCA 
GCCCGAAGCT CCGGGGTGGG CGAGTGTGGA GACCACCCCT GCCTGCCCAA CCCCTGCCTC 
GACGGGGCTC CGTGCCAGGC CCTGGAGGCT GGCATGTTCC ACTGTCAGTG CCCACCTGGC 
CGCTTTGGTC CAACCTGTAG TGGTGACAAG AACCCCTGTG AGCCGAACCC CTGCCATGGG 
GCAGCCCCCT GCCGTGTGCT GCCTGATGGG GAGGCCAAGT GTGAATGCCC CCGAGGACGA 
GGGGGTGCCA TCTGCCAGAC AGTCTCGGAG CGGGATTCTT CCCAGCCCTT CCTGGCTGAC 
TTCCATGGCT TCTCCTACCT GGAGCTGAAA GGGTTGCATA CCTTTGAGCG GGACCTGGGG 
GAGAAGCTGG CACTGGAGGT GGTGTTCCTG GCCCGTGGGC CCAGTGGTTT GCTCCTCTAC 
AATGGGCAGA AAACAGACGG CAAGGGGGAC TTTGTGTCAC TGGCGTTGCA TGACCGCTTC 
TTGGAATTCC GCTATGACCT GGGCAAGGGA GCAGCGGTCA TCAGGAGCAA GGAGCCGGTG 
GCCCTGGACA CCTGGACCAG GGTCTCACTA GAGCGCAATG GCCGCAAGGG TGCCATGCGT 
GTCAGTGATG GGCCTCGCGT GCTGGGAGAG TCCCCGGTGC CACACACTGT CCTCAACCTG 
AAGGAGCCAC TCTATGTTGG AGGGGCCCCT GACTTCAGCA AACTGGCCCG GGCAGCTGCG 
GTATTCTCTG GCTTCGATGG CGCCATCCAA CTGGTCTCCT TGAAAGGCCA CCAGCTGCTG 
TCCCAGGAGC ACGTGGTGCG GTCAGTGGAT GTCTCATCTT TTGCCCACCA CCCTTGTACT 
CAGGCCTCGG GCCACCCATG CCTCAATGGG GCCTCCTGCC TCCCTCGAGA GGCCTCCTAT 
GAGTGCCTGT GCCCCGGGGG CTTCTCAGGG CTTCACTGTG AGAAGGGGCT GATTGAGAAG 
TCAGCAGGGG ATGTGGACGC CCTGGCCTTT GACGGACAGA CCTACGTCGA GTACCTCAAT 
GCCGTGACTG AGAGTGAGAA GGCCCTGCAG AGCAACCACT TTGAGCTGAG CCTGCGCACG 
GAAGCCACTC AGGGGCTGGT GCTGTGGAGC GGCAAGGCCA CGGAGCGGGC CGACTATGTC 
GCACTGGCCA TCGTGGATGG GCGCCTGCAG CTGACCTATG ACCTGGGCTC CCAGCCCGTC 
GTGCTGCGCT CCACCGTGCC GGTCAACACC AACCGCTGGC TACGAGTCAG GGCACATAGG 
GAACAGAGGG AAGGTTCCCT TCAGGTGGGC AATGAGGCTC CCGTGACTGG CTCTTCCCCG 
CTGGGTGCCA CACAGCTAGA CACAGACGGA ACTCTATGGC TGGGTGGCCT GGAGAAGCTG 
CCCATAGGCC AGGCCCTGCC CAAGGCCTAT GGCACGGGCT TTGTGGGCTG CCTGCGGGAT 
GTGGTGGTGG GGCAGCGCCC ACTGCACCTG CTGGAGGATG CTGTCACCAA GCCAGAGCTC 
CGACCCTGTG CCGCCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AGRN
Entrez Gene ID	479574
Full Name	agrin
General protein information	Preferred Names agrin Names agrin
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

Canis lupus familiaris (dog)	AGRN	XP_005620416.1
Homo sapiens (human)	AGRN	NP_940978.2
Rattus norvegicus (Norway rat)	Agrn	NP_786930.1
Caenorhabditis elegans (roundworm)	agr-1	NP_001022152.3
Xenopus tropicalis (tropical clawed frog)	agrn	XP_002936430.2
	AGRN	XP_001088755.2
Mus musculus (house mouse)	Agrn	NP_067617.3
Gallus gallus (chicken)	AGRN	NP_990858.1
Pan troglodytes (chimpanzee)	LOC100609326	XP_003319002.1
Bos taurus (cattle)	AGRN	XP_005217262.1
Danio rerio (zebrafish)	hm:gc12	NP_001170923.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AGRN cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

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