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MELK cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol MELK
Entrez Gene ID 481608
Full Name maternal embryonic leucine zipper kinase
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_022425606.1 XP_022281314.1 maternal embryonic leucine zipper kinase isoform X1
XM_022425607.1 XP_022281315.1 maternal embryonic leucine zipper kinase isoform X2
XM_022425609.1 XP_022281317.1 maternal embryonic leucine zipper kinase isoform X3
XM_014117862.2 XP_013973337.1 maternal embryonic leucine zipper kinase isoform X1
XM_014117862.1 XP_013973337.1 maternal embryonic leucine zipper kinase
XM_022425608.1 XP_022281316.1 maternal embryonic leucine zipper kinase isoform X2
XM_022425610.1 XP_022281318.1 maternal embryonic leucine zipper kinase isoform X4

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) MELK XP_538730.2
Gallus gallus (chicken) MELK NP_001026680.1
Xenopus tropicalis (tropical clawed frog) melk NP_001016390.1
Rattus norvegicus (Norway rat) Melk NP_001102132.1
Pan troglodytes (chimpanzee) MELK XP_001169038.1
Mus musculus (house mouse) Melk NP_034920.2
Danio rerio (zebrafish) melk NP_996771.2
Arabidopsis thaliana (thale cress) CIPK7 NP_188940.1
Homo sapiens (human) MELK NP_055606.1
MELK XP_001115076.2
Bos taurus (cattle) MELK NP_001104730.1
Caenorhabditis elegans (roundworm) pig-1 NP_001023420.2
Arabidopsis thaliana (thale cress) CIPK4 NP_193194.1

Gene Ontology

The following MELK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MELK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe14637 XM_022425606.1
Latest version!		 Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OCe14637 XM_014117862.2
Latest version!		 Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OCe292177 XM_022425608.1
Latest version!		 Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
OCe292177 XM_022425607.1
Latest version!		 Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
OCe292178 XM_022425609.1
Latest version!		 Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
OCe292179 XM_022425610.1
Latest version!		 Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OCe14637 XM_014117862.1 Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe14637
Clone ID Related Accession (Same CDS sequence) XM_022425606.1 , XM_014117862.2 , XM_014117862.1
Accession Version XM_022425606.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product maternal embryonic leucine zipper kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAC ATCCTTACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGA TCAAAACGGA GATTGAGGCT 
TTGAAGAACC TGCGACATCA GCATATATGT CAACTCTACC ACGTGCTAGA GACAGCCAAC 
AAAATATTCA TGGTTCTTGA GTACTGCCCT GGAGGAGAGC TCTTTGACTA CATAATTTCC 
CAGGATCGCC TGTCCGAGGA GGAGACTCGA GTTGTCTTTC GTCAGATACT TTCTGCTGTT 
GCTTACGTAC ACAGCCAGGG CTATGCTCAT AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATA 
CAAGGCAAAT CCTATCTGGG ATCAGAGGCA GATGTTTGGA GTATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GATGATAATG TTATGGCTTT GTACAAGAAG 
ATTATGAGAG GGAAATATGA GGTTCCTAAG TGGCTCTCTT CTAGTAGCAT TCTGCTTCTT 
CAACAAATGC TACAGGTGGA CCCAAAGAAA CGGATTTCTA TGAAAAATCT TTTGAGCCAT 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AGAGCAAGAA TTCTATTATT 
CACCTCGATG AGGATTGCAT AACAGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACA 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CTGCTACCTA TCTTCTGCTT 
CTAGCCAAGA AGGCTCGGGG AAAACCAGTT CGCTTAAGGC TTCCTTCTTT TTCCTGTGGA 
CTAGCCAGTG CTACCCCATT CACAAACATC AAGTCAAAGA ATCTGAGTCT AGAAGATATG 
ACAACAAGTG ATGAAAACTG TGTGGCTGGA TTGATAGACT ATGACTGGTG TGGAGACAGT 
TCATCAACAG GTGTGGCCAC TCCACAAACA CCACAGTTTG CCAAGCATTG GACAGAATCA 
AATGGTCTGG AATCTAAATC ATTAACTCCA GTATTATGCA GAGCATCTGC TGATAAATTA 
AAGAACAAAG AGAATGTATA TATGCCTAAG TCTGCTGTAA AGGATGATGA CTGCTTTGTA 
TTTCCTGAGC CAAAGACTCC AGTTAATAAG AGCCAGTATA AGAGAGAAAT ACTCTGTACA 
CCAAATCATT ACACTACACC CTCAAAAGCT AGAAACCAGT GCCTGAAAGA AACCCCAATT 
AAAATGCCAA TAAATTCAGC AGGGACAGAG AAGTTAATGA CAGGTGTCAT TAGCCCTGAG 
AGGAGATGTC GCTCAGTGGA ACTAGATCTC AACCAAGCAC ATGTAGAGGA CACTCCAAAA 
AGAAAAGGAA CCAAAGTGTT TGGGAGCCTT GAAAGGGGGT TGGATAAGGT TATCACTGTG 
CTTACCAGGA GCAAAAAGAA GGGCTCTGCC AAAGATGGGC CAAGAAGACT AAAGCTTCAC 
TATAATGTGA CTACAACCAG ATTAGTGAAT CCAGATCAAC TCTTGAATGA AATAATGTCT 
ATTCTTCCAA AGAAGCACGT TGACTTTGTA CAAAAGGGCT ATACACTGAA GTGTCAAACA 
CAGTCAGATT TTGGTAAAGT GACAATGCAA TTTGAACTAG AAGTGTGCCA GCTTCAAAAA 
CCCGATGTGG TGGGTATCAG GCGGCAGCGG ATTAAGGGTG ATGCCTGGGT TTACAAGAGA 
TTAGTGGAAG ACATCCTGTC TAGCTGCAAG GTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022281314.1
CDS187..2142
Translation

Target ORF information:

RefSeq Version XM_022425606.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022425606.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAC ATCCTTACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGA TCAAAACGGA GATTGAGGCT 
TTGAAGAACC TGCGACATCA GCATATATGT CAACTCTACC ACGTGCTAGA GACAGCCAAC 
AAAATATTCA TGGTTCTTGA GTACTGCCCT GGAGGAGAGC TCTTTGACTA CATAATTTCC 
CAGGATCGCC TGTCCGAGGA GGAGACTCGA GTTGTCTTTC GTCAGATACT TTCTGCTGTT 
GCTTACGTAC ACAGCCAGGG CTATGCTCAT AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATA 
CAAGGCAAAT CCTATCTGGG ATCAGAGGCA GATGTTTGGA GTATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GATGATAATG TTATGGCTTT GTACAAGAAG 
ATTATGAGAG GGAAATATGA GGTTCCTAAG TGGCTCTCTT CTAGTAGCAT TCTGCTTCTT 
CAACAAATGC TACAGGTGGA CCCAAAGAAA CGGATTTCTA TGAAAAATCT TTTGAGCCAT 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AGAGCAAGAA TTCTATTATT 
CACCTCGATG AGGATTGCAT AACAGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACA 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CTGCTACCTA TCTTCTGCTT 
CTAGCCAAGA AGGCTCGGGG AAAACCAGTT CGCTTAAGGC TTCCTTCTTT TTCCTGTGGA 
CTAGCCAGTG CTACCCCATT CACAAACATC AAGTCAAAGA ATCTGAGTCT AGAAGATATG 
ACAACAAGTG ATGAAAACTG TGTGGCTGGA TTGATAGACT ATGACTGGTG TGGAGACAGT 
TCATCAACAG GTGTGGCCAC TCCACAAACA CCACAGTTTG CCAAGCATTG GACAGAATCA 
AATGGTCTGG AATCTAAATC ATTAACTCCA GTATTATGCA GAGCATCTGC TGATAAATTA 
AAGAACAAAG AGAATGTATA TATGCCTAAG TCTGCTGTAA AGGATGATGA CTGCTTTGTA 
TTTCCTGAGC CAAAGACTCC AGTTAATAAG AGCCAGTATA AGAGAGAAAT ACTCTGTACA 
CCAAATCATT ACACTACACC CTCAAAAGCT AGAAACCAGT GCCTGAAAGA AACCCCAATT 
AAAATGCCAA TAAATTCAGC AGGGACAGAG AAGTTAATGA CAGGTGTCAT TAGCCCTGAG 
AGGAGATGTC GCTCAGTGGA ACTAGATCTC AACCAAGCAC ATGTAGAGGA CACTCCAAAA 
AGAAAAGGAA CCAAAGTGTT TGGGAGCCTT GAAAGGGGGT TGGATAAGGT TATCACTGTG 
CTTACCAGGA GCAAAAAGAA GGGCTCTGCC AAAGATGGGC CAAGAAGACT AAAGCTTCAC 
TATAATGTGA CTACAACCAG ATTAGTGAAT CCAGATCAAC TCTTGAATGA AATAATGTCT 
ATTCTTCCAA AGAAGCACGT TGACTTTGTA CAAAAGGGCT ATACACTGAA GTGTCAAACA 
CAGTCAGATT TTGGTAAAGT GACAATGCAA TTTGAACTAG AAGTGTGCCA GCTTCAAAAA 
CCCGATGTGG TGGGTATCAG GCGGCAGCGG ATTAAGGGTG ATGCCTGGGT TTACAAGAGA 
TTAGTGGAAG ACATCCTGTC TAGCTGCAAG GTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe14637
Clone ID Related Accession (Same CDS sequence) XM_022425606.1 , XM_014117862.2 , XM_014117862.1
Accession Version XM_014117862.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product maternal embryonic leucine zipper kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAC ATCCTTACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGA TCAAAACGGA GATTGAGGCT 
TTGAAGAACC TGCGACATCA GCATATATGT CAACTCTACC ACGTGCTAGA GACAGCCAAC 
AAAATATTCA TGGTTCTTGA GTACTGCCCT GGAGGAGAGC TCTTTGACTA CATAATTTCC 
CAGGATCGCC TGTCCGAGGA GGAGACTCGA GTTGTCTTTC GTCAGATACT TTCTGCTGTT 
GCTTACGTAC ACAGCCAGGG CTATGCTCAT AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATA 
CAAGGCAAAT CCTATCTGGG ATCAGAGGCA GATGTTTGGA GTATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GATGATAATG TTATGGCTTT GTACAAGAAG 
ATTATGAGAG GGAAATATGA GGTTCCTAAG TGGCTCTCTT CTAGTAGCAT TCTGCTTCTT 
CAACAAATGC TACAGGTGGA CCCAAAGAAA CGGATTTCTA TGAAAAATCT TTTGAGCCAT 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AGAGCAAGAA TTCTATTATT 
CACCTCGATG AGGATTGCAT AACAGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACA 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CTGCTACCTA TCTTCTGCTT 
CTAGCCAAGA AGGCTCGGGG AAAACCAGTT CGCTTAAGGC TTCCTTCTTT TTCCTGTGGA 
CTAGCCAGTG CTACCCCATT CACAAACATC AAGTCAAAGA ATCTGAGTCT AGAAGATATG 
ACAACAAGTG ATGAAAACTG TGTGGCTGGA TTGATAGACT ATGACTGGTG TGGAGACAGT 
TCATCAACAG GTGTGGCCAC TCCACAAACA CCACAGTTTG CCAAGCATTG GACAGAATCA 
AATGGTCTGG AATCTAAATC ATTAACTCCA GTATTATGCA GAGCATCTGC TGATAAATTA 
AAGAACAAAG AGAATGTATA TATGCCTAAG TCTGCTGTAA AGGATGATGA CTGCTTTGTA 
TTTCCTGAGC CAAAGACTCC AGTTAATAAG AGCCAGTATA AGAGAGAAAT ACTCTGTACA 
CCAAATCATT ACACTACACC CTCAAAAGCT AGAAACCAGT GCCTGAAAGA AACCCCAATT 
AAAATGCCAA TAAATTCAGC AGGGACAGAG AAGTTAATGA CAGGTGTCAT TAGCCCTGAG 
AGGAGATGTC GCTCAGTGGA ACTAGATCTC AACCAAGCAC ATGTAGAGGA CACTCCAAAA 
AGAAAAGGAA CCAAAGTGTT TGGGAGCCTT GAAAGGGGGT TGGATAAGGT TATCACTGTG 
CTTACCAGGA GCAAAAAGAA GGGCTCTGCC AAAGATGGGC CAAGAAGACT AAAGCTTCAC 
TATAATGTGA CTACAACCAG ATTAGTGAAT CCAGATCAAC TCTTGAATGA AATAATGTCT 
ATTCTTCCAA AGAAGCACGT TGACTTTGTA CAAAAGGGCT ATACACTGAA GTGTCAAACA 
CAGTCAGATT TTGGTAAAGT GACAATGCAA TTTGAACTAG AAGTGTGCCA GCTTCAAAAA 
CCCGATGTGG TGGGTATCAG GCGGCAGCGG ATTAAGGGTG ATGCCTGGGT TTACAAGAGA 
TTAGTGGAAG ACATCCTGTC TAGCTGCAAG GTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013973337.1
CDS320..2275
Translation

Target ORF information:

RefSeq Version XM_014117862.2
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014117862.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAC ATCCTTACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGA TCAAAACGGA GATTGAGGCT 
TTGAAGAACC TGCGACATCA GCATATATGT CAACTCTACC ACGTGCTAGA GACAGCCAAC 
AAAATATTCA TGGTTCTTGA GTACTGCCCT GGAGGAGAGC TCTTTGACTA CATAATTTCC 
CAGGATCGCC TGTCCGAGGA GGAGACTCGA GTTGTCTTTC GTCAGATACT TTCTGCTGTT 
GCTTACGTAC ACAGCCAGGG CTATGCTCAT AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATA 
CAAGGCAAAT CCTATCTGGG ATCAGAGGCA GATGTTTGGA GTATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GATGATAATG TTATGGCTTT GTACAAGAAG 
ATTATGAGAG GGAAATATGA GGTTCCTAAG TGGCTCTCTT CTAGTAGCAT TCTGCTTCTT 
CAACAAATGC TACAGGTGGA CCCAAAGAAA CGGATTTCTA TGAAAAATCT TTTGAGCCAT 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AGAGCAAGAA TTCTATTATT 
CACCTCGATG AGGATTGCAT AACAGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACA 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CTGCTACCTA TCTTCTGCTT 
CTAGCCAAGA AGGCTCGGGG AAAACCAGTT CGCTTAAGGC TTCCTTCTTT TTCCTGTGGA 
CTAGCCAGTG CTACCCCATT CACAAACATC AAGTCAAAGA ATCTGAGTCT AGAAGATATG 
ACAACAAGTG ATGAAAACTG TGTGGCTGGA TTGATAGACT ATGACTGGTG TGGAGACAGT 
TCATCAACAG GTGTGGCCAC TCCACAAACA CCACAGTTTG CCAAGCATTG GACAGAATCA 
AATGGTCTGG AATCTAAATC ATTAACTCCA GTATTATGCA GAGCATCTGC TGATAAATTA 
AAGAACAAAG AGAATGTATA TATGCCTAAG TCTGCTGTAA AGGATGATGA CTGCTTTGTA 
TTTCCTGAGC CAAAGACTCC AGTTAATAAG AGCCAGTATA AGAGAGAAAT ACTCTGTACA 
CCAAATCATT ACACTACACC CTCAAAAGCT AGAAACCAGT GCCTGAAAGA AACCCCAATT 
AAAATGCCAA TAAATTCAGC AGGGACAGAG AAGTTAATGA CAGGTGTCAT TAGCCCTGAG 
AGGAGATGTC GCTCAGTGGA ACTAGATCTC AACCAAGCAC ATGTAGAGGA CACTCCAAAA 
AGAAAAGGAA CCAAAGTGTT TGGGAGCCTT GAAAGGGGGT TGGATAAGGT TATCACTGTG 
CTTACCAGGA GCAAAAAGAA GGGCTCTGCC AAAGATGGGC CAAGAAGACT AAAGCTTCAC 
TATAATGTGA CTACAACCAG ATTAGTGAAT CCAGATCAAC TCTTGAATGA AATAATGTCT 
ATTCTTCCAA AGAAGCACGT TGACTTTGTA CAAAAGGGCT ATACACTGAA GTGTCAAACA 
CAGTCAGATT TTGGTAAAGT GACAATGCAA TTTGAACTAG AAGTGTGCCA GCTTCAAAAA 
CCCGATGTGG TGGGTATCAG GCGGCAGCGG ATTAAGGGTG ATGCCTGGGT TTACAAGAGA 
TTAGTGGAAG ACATCCTGTC TAGCTGCAAG GTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe14637
Clone ID Related Accession (Same CDS sequence) XM_022425606.1 , XM_014117862.2 , XM_014117862.1
Accession Version XM_014117862.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product maternal embryonic leucine zipper kinase
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAC ATCCTTACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGA TCAAAACGGA GATTGAGGCT 
TTGAAGAACC TGCGACATCA GCATATATGT CAACTCTACC ACGTGCTAGA GACAGCCAAC 
AAAATATTCA TGGTTCTTGA GTACTGCCCT GGAGGAGAGC TCTTTGACTA CATAATTTCC 
CAGGATCGCC TGTCCGAGGA GGAGACTCGA GTTGTCTTTC GTCAGATACT TTCTGCTGTT 
GCTTACGTAC ACAGCCAGGG CTATGCTCAT AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATA 
CAAGGCAAAT CCTATCTGGG ATCAGAGGCA GATGTTTGGA GTATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GATGATAATG TTATGGCTTT GTACAAGAAG 
ATTATGAGAG GGAAATATGA GGTTCCTAAG TGGCTCTCTT CTAGTAGCAT TCTGCTTCTT 
CAACAAATGC TACAGGTGGA CCCAAAGAAA CGGATTTCTA TGAAAAATCT TTTGAGCCAT 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AGAGCAAGAA TTCTATTATT 
CACCTCGATG AGGATTGCAT AACAGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACA 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CTGCTACCTA TCTTCTGCTT 
CTAGCCAAGA AGGCTCGGGG AAAACCAGTT CGCTTAAGGC TTCCTTCTTT TTCCTGTGGA 
CTAGCCAGTG CTACCCCATT CACAAACATC AAGTCAAAGA ATCTGAGTCT AGAAGATATG 
ACAACAAGTG ATGAAAACTG TGTGGCTGGA TTGATAGACT ATGACTGGTG TGGAGACAGT 
TCATCAACAG GTGTGGCCAC TCCACAAACA CCACAGTTTG CCAAGCATTG GACAGAATCA 
AATGGTCTGG AATCTAAATC ATTAACTCCA GTATTATGCA GAGCATCTGC TGATAAATTA 
AAGAACAAAG AGAATGTATA TATGCCTAAG TCTGCTGTAA AGGATGATGA CTGCTTTGTA 
TTTCCTGAGC CAAAGACTCC AGTTAATAAG AGCCAGTATA AGAGAGAAAT ACTCTGTACA 
CCAAATCATT ACACTACACC CTCAAAAGCT AGAAACCAGT GCCTGAAAGA AACCCCAATT 
AAAATGCCAA TAAATTCAGC AGGGACAGAG AAGTTAATGA CAGGTGTCAT TAGCCCTGAG 
AGGAGATGTC GCTCAGTGGA ACTAGATCTC AACCAAGCAC ATGTAGAGGA CACTCCAAAA 
AGAAAAGGAA CCAAAGTGTT TGGGAGCCTT GAAAGGGGGT TGGATAAGGT TATCACTGTG 
CTTACCAGGA GCAAAAAGAA GGGCTCTGCC AAAGATGGGC CAAGAAGACT AAAGCTTCAC 
TATAATGTGA CTACAACCAG ATTAGTGAAT CCAGATCAAC TCTTGAATGA AATAATGTCT 
ATTCTTCCAA AGAAGCACGT TGACTTTGTA CAAAAGGGCT ATACACTGAA GTGTCAAACA 
CAGTCAGATT TTGGTAAAGT GACAATGCAA TTTGAACTAG AAGTGTGCCA GCTTCAAAAA 
CCCGATGTGG TGGGTATCAG GCGGCAGCGG ATTAAGGGTG ATGCCTGGGT TTACAAGAGA 
TTAGTGGAAG ACATCCTGTC TAGCTGCAAG GTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013973337.1
CDS352..2307
Translation

Target ORF information:

RefSeq Version XM_014117862.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014117862.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAC ATCCTTACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGA TCAAAACGGA GATTGAGGCT 
TTGAAGAACC TGCGACATCA GCATATATGT CAACTCTACC ACGTGCTAGA GACAGCCAAC 
AAAATATTCA TGGTTCTTGA GTACTGCCCT GGAGGAGAGC TCTTTGACTA CATAATTTCC 
CAGGATCGCC TGTCCGAGGA GGAGACTCGA GTTGTCTTTC GTCAGATACT TTCTGCTGTT 
GCTTACGTAC ACAGCCAGGG CTATGCTCAT AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATA 
CAAGGCAAAT CCTATCTGGG ATCAGAGGCA GATGTTTGGA GTATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GATGATAATG TTATGGCTTT GTACAAGAAG 
ATTATGAGAG GGAAATATGA GGTTCCTAAG TGGCTCTCTT CTAGTAGCAT TCTGCTTCTT 
CAACAAATGC TACAGGTGGA CCCAAAGAAA CGGATTTCTA TGAAAAATCT TTTGAGCCAT 
CCCTGGATCA TGCACGATTA CAACTGTCCT GTTGAGTGGC AGAGCAAGAA TTCTATTATT 
CACCTCGATG AGGATTGCAT AACAGAACTT TCTGTACATC ACAGAAATAA CAGGCAAACA 
ATGGAGGATT TAATTTCACT GTGGCAGTAT GATCACCTCA CTGCTACCTA TCTTCTGCTT 
CTAGCCAAGA AGGCTCGGGG AAAACCAGTT CGCTTAAGGC TTCCTTCTTT TTCCTGTGGA 
CTAGCCAGTG CTACCCCATT CACAAACATC AAGTCAAAGA ATCTGAGTCT AGAAGATATG 
ACAACAAGTG ATGAAAACTG TGTGGCTGGA TTGATAGACT ATGACTGGTG TGGAGACAGT 
TCATCAACAG GTGTGGCCAC TCCACAAACA CCACAGTTTG CCAAGCATTG GACAGAATCA 
AATGGTCTGG AATCTAAATC ATTAACTCCA GTATTATGCA GAGCATCTGC TGATAAATTA 
AAGAACAAAG AGAATGTATA TATGCCTAAG TCTGCTGTAA AGGATGATGA CTGCTTTGTA 
TTTCCTGAGC CAAAGACTCC AGTTAATAAG AGCCAGTATA AGAGAGAAAT ACTCTGTACA 
CCAAATCATT ACACTACACC CTCAAAAGCT AGAAACCAGT GCCTGAAAGA AACCCCAATT 
AAAATGCCAA TAAATTCAGC AGGGACAGAG AAGTTAATGA CAGGTGTCAT TAGCCCTGAG 
AGGAGATGTC GCTCAGTGGA ACTAGATCTC AACCAAGCAC ATGTAGAGGA CACTCCAAAA 
AGAAAAGGAA CCAAAGTGTT TGGGAGCCTT GAAAGGGGGT TGGATAAGGT TATCACTGTG 
CTTACCAGGA GCAAAAAGAA GGGCTCTGCC AAAGATGGGC CAAGAAGACT AAAGCTTCAC 
TATAATGTGA CTACAACCAG ATTAGTGAAT CCAGATCAAC TCTTGAATGA AATAATGTCT 
ATTCTTCCAA AGAAGCACGT TGACTTTGTA CAAAAGGGCT ATACACTGAA GTGTCAAACA 
CAGTCAGATT TTGGTAAAGT GACAATGCAA TTTGAACTAG AAGTGTGCCA GCTTCAAAAA 
CCCGATGTGG TGGGTATCAG GCGGCAGCGG ATTAAGGGTG ATGCCTGGGT TTACAAGAGA 
TTAGTGGAAG ACATCCTGTC TAGCTGCAAG GTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe292177
Clone ID Related Accession (Same CDS sequence) XM_022425607.1 , XM_022425608.1
Accession Version XM_022425607.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product maternal embryonic leucine zipper kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGTAGCTA TAAAAATCAT GGATAAAAAT GCACTAGGGA GTGATTTGCC TCGGATCAAA 
ACGGAGATTG AGGCTTTGAA GAACCTGCGA CATCAGCATA TATGTCAACT CTACCACGTG 
CTAGAGACAG CCAACAAAAT ATTCATGGTT CTTGAGTACT GCCCTGGAGG AGAGCTCTTT 
GACTACATAA TTTCCCAGGA TCGCCTGTCC GAGGAGGAGA CTCGAGTTGT CTTTCGTCAG 
ATACTTTCTG CTGTTGCTTA CGTACACAGC CAGGGCTATG CTCATAGGGA CCTCAAACCA 
GAAAATTTGT TGTTTGATGA ATATCATAAA TTAAAGCTGA TTGACTTTGG TCTCTGTGCA 
AAACCCAAGG GTAACAAGGA CTACCATCTG CAGACGTGCT GTGGGAGTCT TGCTTATGCA 
GCACCTGAAT TAATACAAGG CAAATCCTAT CTGGGATCAG AGGCAGATGT TTGGAGTATG 
GGCATACTCT TATATGTACT TATGTGTGGC TTTCTACCAT TTGATGATGA TAATGTTATG 
GCTTTGTACA AGAAGATTAT GAGAGGGAAA TATGAGGTTC CTAAGTGGCT CTCTTCTAGT 
AGCATTCTGC TTCTTCAACA AATGCTACAG GTGGACCCAA AGAAACGGAT TTCTATGAAA 
AATCTTTTGA GCCATCCCTG GATCATGCAC GATTACAACT GTCCTGTTGA GTGGCAGAGC 
AAGAATTCTA TTATTCACCT CGATGAGGAT TGCATAACAG AACTTTCTGT ACATCACAGA 
AATAACAGGC AAACAATGGA GGATTTAATT TCACTGTGGC AGTATGATCA CCTCACTGCT 
ACCTATCTTC TGCTTCTAGC CAAGAAGGCT CGGGGAAAAC CAGTTCGCTT AAGGCTTCCT 
TCTTTTTCCT GTGGACTAGC CAGTGCTACC CCATTCACAA ACATCAAGTC AAAGAATCTG 
AGTCTAGAAG ATATGACAAC AAGTGATGAA AACTGTGTGG CTGGATTGAT AGACTATGAC 
TGGTGTGGAG ACAGTTCATC AACAGGTGTG GCCACTCCAC AAACACCACA GTTTGCCAAG 
CATTGGACAG AATCAAATGG TCTGGAATCT AAATCATTAA CTCCAGTATT ATGCAGAGCA 
TCTGCTGATA AATTAAAGAA CAAAGAGAAT GTATATATGC CTAAGTCTGC TGTAAAGGAT 
GATGACTGCT TTGTATTTCC TGAGCCAAAG ACTCCAGTTA ATAAGAGCCA GTATAAGAGA 
GAAATACTCT GTACACCAAA TCATTACACT ACACCCTCAA AAGCTAGAAA CCAGTGCCTG 
AAAGAAACCC CAATTAAAAT GCCAATAAAT TCAGCAGGGA CAGAGAAGTT AATGACAGGT 
GTCATTAGCC CTGAGAGGAG ATGTCGCTCA GTGGAACTAG ATCTCAACCA AGCACATGTA 
GAGGACACTC CAAAAAGAAA AGGAACCAAA GTGTTTGGGA GCCTTGAAAG GGGGTTGGAT 
AAGGTTATCA CTGTGCTTAC CAGGAGCAAA AAGAAGGGCT CTGCCAAAGA TGGGCCAAGA 
AGACTAAAGC TTCACTATAA TGTGACTACA ACCAGATTAG TGAATCCAGA TCAACTCTTG 
AATGAAATAA TGTCTATTCT TCCAAAGAAG CACGTTGACT TTGTACAAAA GGGCTATACA 
CTGAAGTGTC AAACACAGTC AGATTTTGGT AAAGTGACAA TGCAATTTGA ACTAGAAGTG 
TGCCAGCTTC AAAAACCCGA TGTGGTGGGT ATCAGGCGGC AGCGGATTAA GGGTGATGCC 
TGGGTTTACA AGAGATTAGT GGAAGACATC CTGTCTAGCT GCAAGGTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022281315.1
CDS326..2176
Translation

Target ORF information:

RefSeq Version XM_022425607.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022425607.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGTAGCTA TAAAAATCAT GGATAAAAAT GCACTAGGGA GTGATTTGCC TCGGATCAAA 
ACGGAGATTG AGGCTTTGAA GAACCTGCGA CATCAGCATA TATGTCAACT CTACCACGTG 
CTAGAGACAG CCAACAAAAT ATTCATGGTT CTTGAGTACT GCCCTGGAGG AGAGCTCTTT 
GACTACATAA TTTCCCAGGA TCGCCTGTCC GAGGAGGAGA CTCGAGTTGT CTTTCGTCAG 
ATACTTTCTG CTGTTGCTTA CGTACACAGC CAGGGCTATG CTCATAGGGA CCTCAAACCA 
GAAAATTTGT TGTTTGATGA ATATCATAAA TTAAAGCTGA TTGACTTTGG TCTCTGTGCA 
AAACCCAAGG GTAACAAGGA CTACCATCTG CAGACGTGCT GTGGGAGTCT TGCTTATGCA 
GCACCTGAAT TAATACAAGG CAAATCCTAT CTGGGATCAG AGGCAGATGT TTGGAGTATG 
GGCATACTCT TATATGTACT TATGTGTGGC TTTCTACCAT TTGATGATGA TAATGTTATG 
GCTTTGTACA AGAAGATTAT GAGAGGGAAA TATGAGGTTC CTAAGTGGCT CTCTTCTAGT 
AGCATTCTGC TTCTTCAACA AATGCTACAG GTGGACCCAA AGAAACGGAT TTCTATGAAA 
AATCTTTTGA GCCATCCCTG GATCATGCAC GATTACAACT GTCCTGTTGA GTGGCAGAGC 
AAGAATTCTA TTATTCACCT CGATGAGGAT TGCATAACAG AACTTTCTGT ACATCACAGA 
AATAACAGGC AAACAATGGA GGATTTAATT TCACTGTGGC AGTATGATCA CCTCACTGCT 
ACCTATCTTC TGCTTCTAGC CAAGAAGGCT CGGGGAAAAC CAGTTCGCTT AAGGCTTCCT 
TCTTTTTCCT GTGGACTAGC CAGTGCTACC CCATTCACAA ACATCAAGTC AAAGAATCTG 
AGTCTAGAAG ATATGACAAC AAGTGATGAA AACTGTGTGG CTGGATTGAT AGACTATGAC 
TGGTGTGGAG ACAGTTCATC AACAGGTGTG GCCACTCCAC AAACACCACA GTTTGCCAAG 
CATTGGACAG AATCAAATGG TCTGGAATCT AAATCATTAA CTCCAGTATT ATGCAGAGCA 
TCTGCTGATA AATTAAAGAA CAAAGAGAAT GTATATATGC CTAAGTCTGC TGTAAAGGAT 
GATGACTGCT TTGTATTTCC TGAGCCAAAG ACTCCAGTTA ATAAGAGCCA GTATAAGAGA 
GAAATACTCT GTACACCAAA TCATTACACT ACACCCTCAA AAGCTAGAAA CCAGTGCCTG 
AAAGAAACCC CAATTAAAAT GCCAATAAAT TCAGCAGGGA CAGAGAAGTT AATGACAGGT 
GTCATTAGCC CTGAGAGGAG ATGTCGCTCA GTGGAACTAG ATCTCAACCA AGCACATGTA 
GAGGACACTC CAAAAAGAAA AGGAACCAAA GTGTTTGGGA GCCTTGAAAG GGGGTTGGAT 
AAGGTTATCA CTGTGCTTAC CAGGAGCAAA AAGAAGGGCT CTGCCAAAGA TGGGCCAAGA 
AGACTAAAGC TTCACTATAA TGTGACTACA ACCAGATTAG TGAATCCAGA TCAACTCTTG 
AATGAAATAA TGTCTATTCT TCCAAAGAAG CACGTTGACT TTGTACAAAA GGGCTATACA 
CTGAAGTGTC AAACACAGTC AGATTTTGGT AAAGTGACAA TGCAATTTGA ACTAGAAGTG 
TGCCAGCTTC AAAAACCCGA TGTGGTGGGT ATCAGGCGGC AGCGGATTAA GGGTGATGCC 
TGGGTTTACA AGAGATTAGT GGAAGACATC CTGTCTAGCT GCAAGGTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe292177
Clone ID Related Accession (Same CDS sequence) XM_022425607.1 , XM_022425608.1
Accession Version XM_022425608.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product maternal embryonic leucine zipper kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGTAGCTA TAAAAATCAT GGATAAAAAT GCACTAGGGA GTGATTTGCC TCGGATCAAA 
ACGGAGATTG AGGCTTTGAA GAACCTGCGA CATCAGCATA TATGTCAACT CTACCACGTG 
CTAGAGACAG CCAACAAAAT ATTCATGGTT CTTGAGTACT GCCCTGGAGG AGAGCTCTTT 
GACTACATAA TTTCCCAGGA TCGCCTGTCC GAGGAGGAGA CTCGAGTTGT CTTTCGTCAG 
ATACTTTCTG CTGTTGCTTA CGTACACAGC CAGGGCTATG CTCATAGGGA CCTCAAACCA 
GAAAATTTGT TGTTTGATGA ATATCATAAA TTAAAGCTGA TTGACTTTGG TCTCTGTGCA 
AAACCCAAGG GTAACAAGGA CTACCATCTG CAGACGTGCT GTGGGAGTCT TGCTTATGCA 
GCACCTGAAT TAATACAAGG CAAATCCTAT CTGGGATCAG AGGCAGATGT TTGGAGTATG 
GGCATACTCT TATATGTACT TATGTGTGGC TTTCTACCAT TTGATGATGA TAATGTTATG 
GCTTTGTACA AGAAGATTAT GAGAGGGAAA TATGAGGTTC CTAAGTGGCT CTCTTCTAGT 
AGCATTCTGC TTCTTCAACA AATGCTACAG GTGGACCCAA AGAAACGGAT TTCTATGAAA 
AATCTTTTGA GCCATCCCTG GATCATGCAC GATTACAACT GTCCTGTTGA GTGGCAGAGC 
AAGAATTCTA TTATTCACCT CGATGAGGAT TGCATAACAG AACTTTCTGT ACATCACAGA 
AATAACAGGC AAACAATGGA GGATTTAATT TCACTGTGGC AGTATGATCA CCTCACTGCT 
ACCTATCTTC TGCTTCTAGC CAAGAAGGCT CGGGGAAAAC CAGTTCGCTT AAGGCTTCCT 
TCTTTTTCCT GTGGACTAGC CAGTGCTACC CCATTCACAA ACATCAAGTC AAAGAATCTG 
AGTCTAGAAG ATATGACAAC AAGTGATGAA AACTGTGTGG CTGGATTGAT AGACTATGAC 
TGGTGTGGAG ACAGTTCATC AACAGGTGTG GCCACTCCAC AAACACCACA GTTTGCCAAG 
CATTGGACAG AATCAAATGG TCTGGAATCT AAATCATTAA CTCCAGTATT ATGCAGAGCA 
TCTGCTGATA AATTAAAGAA CAAAGAGAAT GTATATATGC CTAAGTCTGC TGTAAAGGAT 
GATGACTGCT TTGTATTTCC TGAGCCAAAG ACTCCAGTTA ATAAGAGCCA GTATAAGAGA 
GAAATACTCT GTACACCAAA TCATTACACT ACACCCTCAA AAGCTAGAAA CCAGTGCCTG 
AAAGAAACCC CAATTAAAAT GCCAATAAAT TCAGCAGGGA CAGAGAAGTT AATGACAGGT 
GTCATTAGCC CTGAGAGGAG ATGTCGCTCA GTGGAACTAG ATCTCAACCA AGCACATGTA 
GAGGACACTC CAAAAAGAAA AGGAACCAAA GTGTTTGGGA GCCTTGAAAG GGGGTTGGAT 
AAGGTTATCA CTGTGCTTAC CAGGAGCAAA AAGAAGGGCT CTGCCAAAGA TGGGCCAAGA 
AGACTAAAGC TTCACTATAA TGTGACTACA ACCAGATTAG TGAATCCAGA TCAACTCTTG 
AATGAAATAA TGTCTATTCT TCCAAAGAAG CACGTTGACT TTGTACAAAA GGGCTATACA 
CTGAAGTGTC AAACACAGTC AGATTTTGGT AAAGTGACAA TGCAATTTGA ACTAGAAGTG 
TGCCAGCTTC AAAAACCCGA TGTGGTGGGT ATCAGGCGGC AGCGGATTAA GGGTGATGCC 
TGGGTTTACA AGAGATTAGT GGAAGACATC CTGTCTAGCT GCAAGGTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022281316.1
CDS195..2045
Translation

Target ORF information:

RefSeq Version XM_022425608.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022425608.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGTAGCTA TAAAAATCAT GGATAAAAAT GCACTAGGGA GTGATTTGCC TCGGATCAAA 
ACGGAGATTG AGGCTTTGAA GAACCTGCGA CATCAGCATA TATGTCAACT CTACCACGTG 
CTAGAGACAG CCAACAAAAT ATTCATGGTT CTTGAGTACT GCCCTGGAGG AGAGCTCTTT 
GACTACATAA TTTCCCAGGA TCGCCTGTCC GAGGAGGAGA CTCGAGTTGT CTTTCGTCAG 
ATACTTTCTG CTGTTGCTTA CGTACACAGC CAGGGCTATG CTCATAGGGA CCTCAAACCA 
GAAAATTTGT TGTTTGATGA ATATCATAAA TTAAAGCTGA TTGACTTTGG TCTCTGTGCA 
AAACCCAAGG GTAACAAGGA CTACCATCTG CAGACGTGCT GTGGGAGTCT TGCTTATGCA 
GCACCTGAAT TAATACAAGG CAAATCCTAT CTGGGATCAG AGGCAGATGT TTGGAGTATG 
GGCATACTCT TATATGTACT TATGTGTGGC TTTCTACCAT TTGATGATGA TAATGTTATG 
GCTTTGTACA AGAAGATTAT GAGAGGGAAA TATGAGGTTC CTAAGTGGCT CTCTTCTAGT 
AGCATTCTGC TTCTTCAACA AATGCTACAG GTGGACCCAA AGAAACGGAT TTCTATGAAA 
AATCTTTTGA GCCATCCCTG GATCATGCAC GATTACAACT GTCCTGTTGA GTGGCAGAGC 
AAGAATTCTA TTATTCACCT CGATGAGGAT TGCATAACAG AACTTTCTGT ACATCACAGA 
AATAACAGGC AAACAATGGA GGATTTAATT TCACTGTGGC AGTATGATCA CCTCACTGCT 
ACCTATCTTC TGCTTCTAGC CAAGAAGGCT CGGGGAAAAC CAGTTCGCTT AAGGCTTCCT 
TCTTTTTCCT GTGGACTAGC CAGTGCTACC CCATTCACAA ACATCAAGTC AAAGAATCTG 
AGTCTAGAAG ATATGACAAC AAGTGATGAA AACTGTGTGG CTGGATTGAT AGACTATGAC 
TGGTGTGGAG ACAGTTCATC AACAGGTGTG GCCACTCCAC AAACACCACA GTTTGCCAAG 
CATTGGACAG AATCAAATGG TCTGGAATCT AAATCATTAA CTCCAGTATT ATGCAGAGCA 
TCTGCTGATA AATTAAAGAA CAAAGAGAAT GTATATATGC CTAAGTCTGC TGTAAAGGAT 
GATGACTGCT TTGTATTTCC TGAGCCAAAG ACTCCAGTTA ATAAGAGCCA GTATAAGAGA 
GAAATACTCT GTACACCAAA TCATTACACT ACACCCTCAA AAGCTAGAAA CCAGTGCCTG 
AAAGAAACCC CAATTAAAAT GCCAATAAAT TCAGCAGGGA CAGAGAAGTT AATGACAGGT 
GTCATTAGCC CTGAGAGGAG ATGTCGCTCA GTGGAACTAG ATCTCAACCA AGCACATGTA 
GAGGACACTC CAAAAAGAAA AGGAACCAAA GTGTTTGGGA GCCTTGAAAG GGGGTTGGAT 
AAGGTTATCA CTGTGCTTAC CAGGAGCAAA AAGAAGGGCT CTGCCAAAGA TGGGCCAAGA 
AGACTAAAGC TTCACTATAA TGTGACTACA ACCAGATTAG TGAATCCAGA TCAACTCTTG 
AATGAAATAA TGTCTATTCT TCCAAAGAAG CACGTTGACT TTGTACAAAA GGGCTATACA 
CTGAAGTGTC AAACACAGTC AGATTTTGGT AAAGTGACAA TGCAATTTGA ACTAGAAGTG 
TGCCAGCTTC AAAAACCCGA TGTGGTGGGT ATCAGGCGGC AGCGGATTAA GGGTGATGCC 
TGGGTTTACA AGAGATTAGT GGAAGACATC CTGTCTAGCT GCAAGGTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe292178
Clone ID Related Accession (Same CDS sequence) XM_022425609.1
Accession Version XM_022425609.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product maternal embryonic leucine zipper kinase isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAC ATCCTTACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGA TCAAAACGGA GATTGAGGCT 
TTGAAGAACC TGCGACATCA GCATATATGT CAACTCTACC ACGTGCTAGA GACAGCCAAC 
AAAATATTCA TGGTTCTTGA GTACTGCCCT GGAGGAGAGC TCTTTGACTA CATAATTTCC 
CAGGATCGCC TGTCCGAGGA GGAGACTCGA GTTGTCTTTC GTCAGATACT TTCTGCTGTT 
GCTTACGTAC ACAGCCAGGG CTATGCTCAT AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATA 
CAAGGCAAAT CCTATCTGGG ATCAGAGGCA GATGTTTGGA GTATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GATGATAATG TTATGGCTTT GTACAAGAAG 
ATTATGATTA TTCACCTCGA TGAGGATTGC ATAACAGAAC TTTCTGTACA TCACAGAAAT 
AACAGGCAAA CAATGGAGGA TTTAATTTCA CTGTGGCAGT ATGATCACCT CACTGCTACC 
TATCTTCTGC TTCTAGCCAA GAAGGCTCGG GGAAAACCAG TTCGCTTAAG GCTTCCTTCT 
TTTTCCTGTG GACTAGCCAG TGCTACCCCA TTCACAAACA TCAAGTCAAA GAATCTGAGT 
CTAGAAGATA TGACAACAAG TGATGAAAAC TGTGTGGCTG GATTGATAGA CTATGACTGG 
TGTGGAGACA GTTCATCAAC AGGTGTGGCC ACTCCACAAA CACCACAGTT TGCCAAGCAT 
TGGACAGAAT CAAATGGTCT GGAATCTAAA TCATTAACTC CAGTATTATG CAGAGCATCT 
GCTGATAAAT TAAAGAACAA AGAGAATGTA TATATGCCTA AGTCTGCTGT AAAGGATGAT 
GACTGCTTTG TATTTCCTGA GCCAAAGACT CCAGTTAATA AGAGCCAGTA TAAGAGAGAA 
ATACTCTGTA CACCAAATCA TTACACTACA CCCTCAAAAG CTAGAAACCA GTGCCTGAAA 
GAAACCCCAA TTAAAATGCC AATAAATTCA GCAGGGACAG AGAAGTTAAT GACAGGTGTC 
ATTAGCCCTG AGAGGAGATG TCGCTCAGTG GAACTAGATC TCAACCAAGC ACATGTAGAG 
GACACTCCAA AAAGAAAAGG AACCAAAGTG TTTGGGAGCC TTGAAAGGGG GTTGGATAAG 
GTTATCACTG TGCTTACCAG GAGCAAAAAG AAGGGCTCTG CCAAAGATGG GCCAAGAAGA 
CTAAAGCTTC ACTATAATGT GACTACAACC AGATTAGTGA ATCCAGATCA ACTCTTGAAT 
GAAATAATGT CTATTCTTCC AAAGAAGCAC GTTGACTTTG TACAAAAGGG CTATACACTG 
AAGTGTCAAA CACAGTCAGA TTTTGGTAAA GTGACAATGC AATTTGAACT AGAAGTGTGC 
CAGCTTCAAA AACCCGATGT GGTGGGTATC AGGCGGCAGC GGATTAAGGG TGATGCCTGG 
GTTTACAAGA GATTAGTGGA AGACATCCTG TCTAGCTGCA AGGTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022281317.1
CDS188..1975
Translation

Target ORF information:

RefSeq Version XM_022425609.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022425609.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAAGATT ATGATGAACT TCTCAAATAC TATGAATTAT ATGAAACTAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT TGCCTGCCAC ATCCTTACTG GAGAAATGGT AGCTATAAAA 
ATCATGGATA AAAATGCACT AGGGAGTGAT TTGCCTCGGA TCAAAACGGA GATTGAGGCT 
TTGAAGAACC TGCGACATCA GCATATATGT CAACTCTACC ACGTGCTAGA GACAGCCAAC 
AAAATATTCA TGGTTCTTGA GTACTGCCCT GGAGGAGAGC TCTTTGACTA CATAATTTCC 
CAGGATCGCC TGTCCGAGGA GGAGACTCGA GTTGTCTTTC GTCAGATACT TTCTGCTGTT 
GCTTACGTAC ACAGCCAGGG CTATGCTCAT AGGGACCTCA AACCAGAAAA TTTGTTGTTT 
GATGAATATC ATAAATTAAA GCTGATTGAC TTTGGTCTCT GTGCAAAACC CAAGGGTAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGTCTTGCTT ATGCAGCACC TGAATTAATA 
CAAGGCAAAT CCTATCTGGG ATCAGAGGCA GATGTTTGGA GTATGGGCAT ACTCTTATAT 
GTACTTATGT GTGGCTTTCT ACCATTTGAT GATGATAATG TTATGGCTTT GTACAAGAAG 
ATTATGATTA TTCACCTCGA TGAGGATTGC ATAACAGAAC TTTCTGTACA TCACAGAAAT 
AACAGGCAAA CAATGGAGGA TTTAATTTCA CTGTGGCAGT ATGATCACCT CACTGCTACC 
TATCTTCTGC TTCTAGCCAA GAAGGCTCGG GGAAAACCAG TTCGCTTAAG GCTTCCTTCT 
TTTTCCTGTG GACTAGCCAG TGCTACCCCA TTCACAAACA TCAAGTCAAA GAATCTGAGT 
CTAGAAGATA TGACAACAAG TGATGAAAAC TGTGTGGCTG GATTGATAGA CTATGACTGG 
TGTGGAGACA GTTCATCAAC AGGTGTGGCC ACTCCACAAA CACCACAGTT TGCCAAGCAT 
TGGACAGAAT CAAATGGTCT GGAATCTAAA TCATTAACTC CAGTATTATG CAGAGCATCT 
GCTGATAAAT TAAAGAACAA AGAGAATGTA TATATGCCTA AGTCTGCTGT AAAGGATGAT 
GACTGCTTTG TATTTCCTGA GCCAAAGACT CCAGTTAATA AGAGCCAGTA TAAGAGAGAA 
ATACTCTGTA CACCAAATCA TTACACTACA CCCTCAAAAG CTAGAAACCA GTGCCTGAAA 
GAAACCCCAA TTAAAATGCC AATAAATTCA GCAGGGACAG AGAAGTTAAT GACAGGTGTC 
ATTAGCCCTG AGAGGAGATG TCGCTCAGTG GAACTAGATC TCAACCAAGC ACATGTAGAG 
GACACTCCAA AAAGAAAAGG AACCAAAGTG TTTGGGAGCC TTGAAAGGGG GTTGGATAAG 
GTTATCACTG TGCTTACCAG GAGCAAAAAG AAGGGCTCTG CCAAAGATGG GCCAAGAAGA 
CTAAAGCTTC ACTATAATGT GACTACAACC AGATTAGTGA ATCCAGATCA ACTCTTGAAT 
GAAATAATGT CTATTCTTCC AAAGAAGCAC GTTGACTTTG TACAAAAGGG CTATACACTG 
AAGTGTCAAA CACAGTCAGA TTTTGGTAAA GTGACAATGC AATTTGAACT AGAAGTGTGC 
CAGCTTCAAA AACCCGATGT GGTGGGTATC AGGCGGCAGC GGATTAAGGG TGATGCCTGG 
GTTTACAAGA GATTAGTGGA AGACATCCTG TCTAGCTGCA AGGTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe292179
Clone ID Related Accession (Same CDS sequence) XM_022425610.1
Accession Version XM_022425610.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1707bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product maternal embryonic leucine zipper kinase isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGTTCTTG AGTACTGCCC TGGAGGAGAG CTCTTTGACT ACATAATTTC CCAGGATCGC 
CTGTCCGAGG AGGAGACTCG AGTTGTCTTT CGTCAGATAC TTTCTGCTGT TGCTTACGTA 
CACAGCCAGG GCTATGCTCA TAGGGACCTC AAACCAGAAA ATTTGTTGTT TGATGAATAT 
CATAAATTAA AGCTGATTGA CTTTGGTCTC TGTGCAAAAC CCAAGGGTAA CAAGGACTAC 
CATCTGCAGA CGTGCTGTGG GAGTCTTGCT TATGCAGCAC CTGAATTAAT ACAAGGCAAA 
TCCTATCTGG GATCAGAGGC AGATGTTTGG AGTATGGGCA TACTCTTATA TGTACTTATG 
TGTGGCTTTC TACCATTTGA TGATGATAAT GTTATGGCTT TGTACAAGAA GATTATGAGA 
GGGAAATATG AGGTTCCTAA GTGGCTCTCT TCTAGTAGCA TTCTGCTTCT TCAACAAATG 
CTACAGGTGG ACCCAAAGAA ACGGATTTCT ATGAAAAATC TTTTGAGCCA TCCCTGGATC 
ATGCACGATT ACAACTGTCC TGTTGAGTGG CAGAGCAAGA ATTCTATTAT TCACCTCGAT 
GAGGATTGCA TAACAGAACT TTCTGTACAT CACAGAAATA ACAGGCAAAC AATGGAGGAT 
TTAATTTCAC TGTGGCAGTA TGATCACCTC ACTGCTACCT ATCTTCTGCT TCTAGCCAAG 
AAGGCTCGGG GAAAACCAGT TCGCTTAAGG CTTCCTTCTT TTTCCTGTGG ACTAGCCAGT 
GCTACCCCAT TCACAAACAT CAAGTCAAAG AATCTGAGTC TAGAAGATAT GACAACAAGT 
GATGAAAACT GTGTGGCTGG ATTGATAGAC TATGACTGGT GTGGAGACAG TTCATCAACA 
GGTGTGGCCA CTCCACAAAC ACCACAGTTT GCCAAGCATT GGACAGAATC AAATGGTCTG 
GAATCTAAAT CATTAACTCC AGTATTATGC AGAGCATCTG CTGATAAATT AAAGAACAAA 
GAGAATGTAT ATATGCCTAA GTCTGCTGTA AAGGATGATG ACTGCTTTGT ATTTCCTGAG 
CCAAAGACTC CAGTTAATAA GAGCCAGTAT AAGAGAGAAA TACTCTGTAC ACCAAATCAT 
TACACTACAC CCTCAAAAGC TAGAAACCAG TGCCTGAAAG AAACCCCAAT TAAAATGCCA 
ATAAATTCAG CAGGGACAGA GAAGTTAATG ACAGGTGTCA TTAGCCCTGA GAGGAGATGT 
CGCTCAGTGG AACTAGATCT CAACCAAGCA CATGTAGAGG ACACTCCAAA AAGAAAAGGA 
ACCAAAGTGT TTGGGAGCCT TGAAAGGGGG TTGGATAAGG TTATCACTGT GCTTACCAGG 
AGCAAAAAGA AGGGCTCTGC CAAAGATGGG CCAAGAAGAC TAAAGCTTCA CTATAATGTG 
ACTACAACCA GATTAGTGAA TCCAGATCAA CTCTTGAATG AAATAATGTC TATTCTTCCA 
AAGAAGCACG TTGACTTTGT ACAAAAGGGC TATACACTGA AGTGTCAAAC ACAGTCAGAT 
TTTGGTAAAG TGACAATGCA ATTTGAACTA GAAGTGTGCC AGCTTCAAAA ACCCGATGTG 
GTGGGTATCA GGCGGCAGCG GATTAAGGGT GATGCCTGGG TTTACAAGAG ATTAGTGGAA 
GACATCCTGT CTAGCTGCAA GGTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022281318.1
CDS253..1959
Translation

Target ORF information:

RefSeq Version XM_022425610.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris maternal embryonic leucine zipper kinase (MELK), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022425610.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGTTCTTG AGTACTGCCC TGGAGGAGAG CTCTTTGACT ACATAATTTC CCAGGATCGC 
CTGTCCGAGG AGGAGACTCG AGTTGTCTTT CGTCAGATAC TTTCTGCTGT TGCTTACGTA 
CACAGCCAGG GCTATGCTCA TAGGGACCTC AAACCAGAAA ATTTGTTGTT TGATGAATAT 
CATAAATTAA AGCTGATTGA CTTTGGTCTC TGTGCAAAAC CCAAGGGTAA CAAGGACTAC 
CATCTGCAGA CGTGCTGTGG GAGTCTTGCT TATGCAGCAC CTGAATTAAT ACAAGGCAAA 
TCCTATCTGG GATCAGAGGC AGATGTTTGG AGTATGGGCA TACTCTTATA TGTACTTATG 
TGTGGCTTTC TACCATTTGA TGATGATAAT GTTATGGCTT TGTACAAGAA GATTATGAGA 
GGGAAATATG AGGTTCCTAA GTGGCTCTCT TCTAGTAGCA TTCTGCTTCT TCAACAAATG 
CTACAGGTGG ACCCAAAGAA ACGGATTTCT ATGAAAAATC TTTTGAGCCA TCCCTGGATC 
ATGCACGATT ACAACTGTCC TGTTGAGTGG CAGAGCAAGA ATTCTATTAT TCACCTCGAT 
GAGGATTGCA TAACAGAACT TTCTGTACAT CACAGAAATA ACAGGCAAAC AATGGAGGAT 
TTAATTTCAC TGTGGCAGTA TGATCACCTC ACTGCTACCT ATCTTCTGCT TCTAGCCAAG 
AAGGCTCGGG GAAAACCAGT TCGCTTAAGG CTTCCTTCTT TTTCCTGTGG ACTAGCCAGT 
GCTACCCCAT TCACAAACAT CAAGTCAAAG AATCTGAGTC TAGAAGATAT GACAACAAGT 
GATGAAAACT GTGTGGCTGG ATTGATAGAC TATGACTGGT GTGGAGACAG TTCATCAACA 
GGTGTGGCCA CTCCACAAAC ACCACAGTTT GCCAAGCATT GGACAGAATC AAATGGTCTG 
GAATCTAAAT CATTAACTCC AGTATTATGC AGAGCATCTG CTGATAAATT AAAGAACAAA 
GAGAATGTAT ATATGCCTAA GTCTGCTGTA AAGGATGATG ACTGCTTTGT ATTTCCTGAG 
CCAAAGACTC CAGTTAATAA GAGCCAGTAT AAGAGAGAAA TACTCTGTAC ACCAAATCAT 
TACACTACAC CCTCAAAAGC TAGAAACCAG TGCCTGAAAG AAACCCCAAT TAAAATGCCA 
ATAAATTCAG CAGGGACAGA GAAGTTAATG ACAGGTGTCA TTAGCCCTGA GAGGAGATGT 
CGCTCAGTGG AACTAGATCT CAACCAAGCA CATGTAGAGG ACACTCCAAA AAGAAAAGGA 
ACCAAAGTGT TTGGGAGCCT TGAAAGGGGG TTGGATAAGG TTATCACTGT GCTTACCAGG 
AGCAAAAAGA AGGGCTCTGC CAAAGATGGG CCAAGAAGAC TAAAGCTTCA CTATAATGTG 
ACTACAACCA GATTAGTGAA TCCAGATCAA CTCTTGAATG AAATAATGTC TATTCTTCCA 
AAGAAGCACG TTGACTTTGT ACAAAAGGGC TATACACTGA AGTGTCAAAC ACAGTCAGAT 
TTTGGTAAAG TGACAATGCA ATTTGAACTA GAAGTGTGCC AGCTTCAAAA ACCCGATGTG 
GTGGGTATCA GGCGGCAGCG GATTAAGGGT GATGCCTGGG TTTACAAGAG ATTAGTGGAA 
GACATCCTGT CTAGCTGCAA GGTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed