COL22A1 cDNA ORF clone, Canis lupus familiaris(dog)

  • Gene

  • Clones

  • gRNAs

The following COL22A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL22A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe16056 XM_539177.6
Latest version!
Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe16056 XM_005627990.3
Latest version!
Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe16056 XM_014118854.2
Latest version!
Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe292864 XM_022426740.1
Latest version!
Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe292865 XM_022426741.1
Latest version!
Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe292866 XM_022426742.1
Latest version!
Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe292867 XM_022426743.1
Latest version!
Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OCe16056 XM_005627990.2 Canis lupus familiaris collagen, type XXII, alpha 1 (COL22A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe16056 XM_014118854.1 Canis lupus familiaris collagen, type XXII, alpha 1 (COL22A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe16056 XM_539177.5 Canis lupus familiaris collagen, type XXII, alpha 1 (COL22A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe16056
    Clone ID Related Accession (Same CDS sequence) XM_539177.5 , XM_005627990.3 , XM_014118854.2 , XM_005627990.2 , XM_014118854.1 , XM_539177.6
    Accession Version XM_539177.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-1(XXII) chain
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_539177.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_539177.3
    CDS495..5333
    Translation

    Target ORF information:

    RefSeq Version XM_539177.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type XXII, alpha 1 (COL22A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_539177.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16056
    Clone ID Related Accession (Same CDS sequence) XM_539177.5 , XM_005627990.3 , XM_014118854.2 , XM_005627990.2 , XM_014118854.1 , XM_539177.6
    Accession Version XM_005627990.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-1(XXII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627990.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005628047.1
    CDS623..5461
    Translation

    Target ORF information:

    RefSeq Version XM_005627990.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627990.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16056
    Clone ID Related Accession (Same CDS sequence) XM_539177.5 , XM_005627990.3 , XM_014118854.2 , XM_005627990.2 , XM_014118854.1 , XM_539177.6
    Accession Version XM_014118854.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-1(XXII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974329.1
    CDS619..5457
    Translation

    Target ORF information:

    RefSeq Version XM_014118854.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118854.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16056
    Clone ID Related Accession (Same CDS sequence) XM_539177.5 , XM_005627990.3 , XM_014118854.2 , XM_005627990.2 , XM_014118854.1 , XM_539177.6
    Accession Version XM_005627990.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-1(XXII) chain
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005628047.1
    CDS595..5433
    Translation

    Target ORF information:

    RefSeq Version XM_005627990.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type XXII, alpha 1 (COL22A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627990.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16056
    Clone ID Related Accession (Same CDS sequence) XM_539177.5 , XM_005627990.3 , XM_014118854.2 , XM_005627990.2 , XM_014118854.1 , XM_539177.6
    Accession Version XM_014118854.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-1(XXII) chain
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974329.1
    CDS591..5429
    Translation

    Target ORF information:

    RefSeq Version XM_014118854.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type XXII, alpha 1 (COL22A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe16056
    Clone ID Related Accession (Same CDS sequence) XM_539177.5 , XM_005627990.3 , XM_014118854.2 , XM_005627990.2 , XM_014118854.1 , XM_539177.6
    Accession Version XM_539177.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-1(XXII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_539177.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_539177.3
    CDS523..5361
    Translation

    Target ORF information:

    RefSeq Version XM_539177.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_539177.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGTCAACCCG GGGAACCTGG CTATGCTAAA
    GACGGGCTCC CCGGGGCTCC CGGCCCTCAA GGGGAGACCG GACCAGCGGG ACACCCTGGC
    CCCCCGGGAC CGCCCGGGCC CCCTGGCCAG TGCGACCCCT CCCAGTGTGC CTACTTTGCC
    AGCCTCGCTG CACGGCCCAG TAACGTGAAG GGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292864
    Clone ID Related Accession (Same CDS sequence) XM_022426740.1
    Accession Version XM_022426740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-1(XXII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGTGAAGCT GGTCCTGCAG GCCCCCCTGG ATTACCTGGC
    ACTACATCCC TGTTCACACC GCACCCAAGA ATGCCAGGAG AGCAAGGGCC CAAAGGAGAG
    AAGGGTGATC CTGGCGTGCC TGGGGAACCG GGATTGCAGG GCCATCCCGG AGAACTGGGG
    CCTCGGGGAC CCACTGGAGC GCCGGGTGCC AAAGGACAGG AAGGCCCACG CGGCGCACCC
    GGATCAACTG GAAACCCCGG CGCTCCTGGA ACTGTAGGCC CCCCTGGTGC CAGCGGCCCC
    CCGGGAAGTG TGGGCCCCCC TGGCATCAGA GGCCCCCCTG GGAAAGATGG AGAGCGTGGA
    GAGAAGGGGG CGACAGGTGA AGAAGGGAGT CCAGGGCCAG CTGGTCCCAG GGGTGATCCC
    GGGGCTCCTG GGCTCCCGGG GACCCCCGGA CAGGGAAAAG ACGGAGAGCC GGGGCTCCGT
    GGGCCACCTG GATCACCTGG GCCTCTAGGA ATCAAGGGGG ACCGAGGTGC ACCTGGGATC
    CCTGGCTCTC CTGGCAACCG TGGCGACCCA GGCATCGGAG TAGCTGGCCC TCCTGGCCCT
    TCTGGACCAC CAGGAGACAA AGGGCCCCCG GGATCCCGAG GCTTACCCGG ATTCCCCGGC
    CCCCAGGGAC CAGCTGGCCA GGATGGTGTA CCAGGAAATC CCGGAGAAAG AGGGCCTCCT
    GGCAAGCCTG GTCCCTCCTC ACTGCTGTCT CCAGGGGACA TAAATCTCTT GGTTAAGGAT
    GTGTGCAATG ACTGCCCTCC AGGACCCCCG GGCCTCCCTG GCCTCCCAGG TTTTAAAGGG
    GACAAAGGTC TCCCAGGAAA GCCAGGGAGA GAAGGCACAG AAGGCAAAAA AGGAGATGCT
    GGGCCCAGGG GCCTCCCAGG GCCTCCAGGA ATAGCTGGAC CACAGGGAAG TAAAGGGGAG
    CGTGGTGCAG ATGGTGAAAT TGGACAGAAA GGTGACCAGG GCCATCCTGG AGTTCCCGGT
    TTCATGGGTC CCCCGGGGAA CCCTGGGCCA CGGGGAGCAG ATGGAATTGC CGGAGCTGCT
    GGCCCTCCAG GGATCCAAGG GCCGCAGGGG AAAGAAGGTC CTCCTGGTCC CCAAGGCCCA
    TTTGGATTAC CAGGAATCCC AGGAGAAGAA GGCAAAGAGG GCAGAGATGG AAAACCAGGT
    CCCCCCGGCG AGCCGGGCAA AATGGGAGAG CCAGGTCTGC CAGGACTGGA GGGAGCCCGA
    GGCCCGCCTG GCTTCAAGGG ACACATGGGT GACTCCGGCG CACCTGGTCC CCGGGGAGAG
    CCTGGTGCCA TGGGGCCCCC TGGCCGAGAA GGGTCCCCGG GAAAAGACGG CGACACTGGA
    CCCACAGGGC CCCAGGGCCC CCAAGGACTG AGGGGCCCAC CGGGTAAGAA CGGATCGCCG
    GGATCTCCGG GAGACCCTGG TCCTGCAGGA AGTCCTGGCC AGAAGGGAAA CAAAGGAGAC
    CATGGCAGCC CGGGACTCCC TGGCTTTCTG GGCCCTCGGG GGCCTCAGGG CGAGCCCGGA
    GACAAGGGAG CCCCGGGCAA GGAGGGCATC CCTGGGAAGC CCGGAGAGCC AGGACCCAAA
    GGAGACAGGG GAGATCCCGG GAACAGAGGT GACAAAGGAC CTCCCGGTGG GAAGGGCCAG
    CCGGGCGACC CTGGAATCCC TGGTCACAAA GGCCTCACGG GCCTGATGGG CCCCCAAGGA
    CCGCCCGGGG AGAATGGAGT GGCCGGGCCC CCAGGGCCTC CGGGCCAGCC AGGATTTCCA
    GGACTCAGGG GGGAGTCCCC CTCCATGGAC ACGCTGCGAC GGCTCATCCA AGAGGAGCTT
    GAGAAACAGC TGGAAACCAG ACTCGCCTAC CTCCTGGCCC AGAGGCCGCC GGTGCACATG
    AAGTCATCCC AAGGCCGACC CGGGCCCCCG GGGCCCCCGG GAAAAGATGG GCTTCCAGGC
    CGAGCAGGCC CCGTGGGGGA GCCAGGACGG CCCGGGCAGG GGGGTCTGGA AGGACCCTCT
    GGACCCATGG GTCCCAAAGG TGAGCGAGGA GCCAAAGGAG ACCCAGGTGC GCCTGGAGTG
    GGCCTCCGGG GCGAGATGGG CCCGCCCGGC GTCCCAGGTC AACCCGGGGA ACCTGGCTAT
    GCTAAAGACG GGCTCCCCGG GGCTCCCGGC CCTCAAGGGG AGACCGGACC AGCGGGACAC
    CCTGGCCCCC CGGGACCGCC CGGGCCCCCT GGCCAGTGCG ACCCCTCCCA GTGTGCCTAC
    TTTGCCAGCC TCGCTGCACG GCCCAGTAAC GTGAAGGGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282448.1
    CDS623..5407
    Translation

    Target ORF information:

    RefSeq Version XM_022426740.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGTGAAGCT GGTCCTGCAG GCCCCCCTGG ATTACCTGGC
    ACTACATCCC TGTTCACACC GCACCCAAGA ATGCCAGGAG AGCAAGGGCC CAAAGGAGAG
    AAGGGTGATC CTGGCGTGCC TGGGGAACCG GGATTGCAGG GCCATCCCGG AGAACTGGGG
    CCTCGGGGAC CCACTGGAGC GCCGGGTGCC AAAGGACAGG AAGGCCCACG CGGCGCACCC
    GGATCAACTG GAAACCCCGG CGCTCCTGGA ACTGTAGGCC CCCCTGGTGC CAGCGGCCCC
    CCGGGAAGTG TGGGCCCCCC TGGCATCAGA GGCCCCCCTG GGAAAGATGG AGAGCGTGGA
    GAGAAGGGGG CGACAGGTGA AGAAGGGAGT CCAGGGCCAG CTGGTCCCAG GGGTGATCCC
    GGGGCTCCTG GGCTCCCGGG GACCCCCGGA CAGGGAAAAG ACGGAGAGCC GGGGCTCCGT
    GGGCCACCTG GATCACCTGG GCCTCTAGGA ATCAAGGGGG ACCGAGGTGC ACCTGGGATC
    CCTGGCTCTC CTGGCAACCG TGGCGACCCA GGCATCGGAG TAGCTGGCCC TCCTGGCCCT
    TCTGGACCAC CAGGAGACAA AGGGCCCCCG GGATCCCGAG GCTTACCCGG ATTCCCCGGC
    CCCCAGGGAC CAGCTGGCCA GGATGGTGTA CCAGGAAATC CCGGAGAAAG AGGGCCTCCT
    GGCAAGCCTG GTCCCTCCTC ACTGCTGTCT CCAGGGGACA TAAATCTCTT GGTTAAGGAT
    GTGTGCAATG ACTGCCCTCC AGGACCCCCG GGCCTCCCTG GCCTCCCAGG TTTTAAAGGG
    GACAAAGGTC TCCCAGGAAA GCCAGGGAGA GAAGGCACAG AAGGCAAAAA AGGAGATGCT
    GGGCCCAGGG GCCTCCCAGG GCCTCCAGGA ATAGCTGGAC CACAGGGAAG TAAAGGGGAG
    CGTGGTGCAG ATGGTGAAAT TGGACAGAAA GGTGACCAGG GCCATCCTGG AGTTCCCGGT
    TTCATGGGTC CCCCGGGGAA CCCTGGGCCA CGGGGAGCAG ATGGAATTGC CGGAGCTGCT
    GGCCCTCCAG GGATCCAAGG GCCGCAGGGG AAAGAAGGTC CTCCTGGTCC CCAAGGCCCA
    TTTGGATTAC CAGGAATCCC AGGAGAAGAA GGCAAAGAGG GCAGAGATGG AAAACCAGGT
    CCCCCCGGCG AGCCGGGCAA AATGGGAGAG CCAGGTCTGC CAGGACTGGA GGGAGCCCGA
    GGCCCGCCTG GCTTCAAGGG ACACATGGGT GACTCCGGCG CACCTGGTCC CCGGGGAGAG
    CCTGGTGCCA TGGGGCCCCC TGGCCGAGAA GGGTCCCCGG GAAAAGACGG CGACACTGGA
    CCCACAGGGC CCCAGGGCCC CCAAGGACTG AGGGGCCCAC CGGGTAAGAA CGGATCGCCG
    GGATCTCCGG GAGACCCTGG TCCTGCAGGA AGTCCTGGCC AGAAGGGAAA CAAAGGAGAC
    CATGGCAGCC CGGGACTCCC TGGCTTTCTG GGCCCTCGGG GGCCTCAGGG CGAGCCCGGA
    GACAAGGGAG CCCCGGGCAA GGAGGGCATC CCTGGGAAGC CCGGAGAGCC AGGACCCAAA
    GGAGACAGGG GAGATCCCGG GAACAGAGGT GACAAAGGAC CTCCCGGTGG GAAGGGCCAG
    CCGGGCGACC CTGGAATCCC TGGTCACAAA GGCCTCACGG GCCTGATGGG CCCCCAAGGA
    CCGCCCGGGG AGAATGGAGT GGCCGGGCCC CCAGGGCCTC CGGGCCAGCC AGGATTTCCA
    GGACTCAGGG GGGAGTCCCC CTCCATGGAC ACGCTGCGAC GGCTCATCCA AGAGGAGCTT
    GAGAAACAGC TGGAAACCAG ACTCGCCTAC CTCCTGGCCC AGAGGCCGCC GGTGCACATG
    AAGTCATCCC AAGGCCGACC CGGGCCCCCG GGGCCCCCGG GAAAAGATGG GCTTCCAGGC
    CGAGCAGGCC CCGTGGGGGA GCCAGGACGG CCCGGGCAGG GGGGTCTGGA AGGACCCTCT
    GGACCCATGG GTCCCAAAGG TGAGCGAGGA GCCAAAGGAG ACCCAGGTGC GCCTGGAGTG
    GGCCTCCGGG GCGAGATGGG CCCGCCCGGC GTCCCAGGTC AACCCGGGGA ACCTGGCTAT
    GCTAAAGACG GGCTCCCCGG GGCTCCCGGC CCTCAAGGGG AGACCGGACC AGCGGGACAC
    CCTGGCCCCC CGGGACCGCC CGGGCCCCCT GGCCAGTGCG ACCCCTCCCA GTGTGCCTAC
    TTTGCCAGCC TCGCTGCACG GCCCAGTAAC GTGAAGGGCC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292865
    Clone ID Related Accession (Same CDS sequence) XM_022426741.1
    Accession Version XM_022426741.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-1(XXII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG TGAAGCTGGT CCTGCAGGCC CCCCTGGATT ACCTGGCACT
    ACATCCCTGT TCACACCGCA CCCAAGAATG CCAGGAGAGC AAGGGCCCAA AGGAGAGAAG
    GGTGATCCTG GCGTGCCTGG GGAACCGGGA TTGCAGGGCC ATCCCGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGAGCGCC GGGTGCCAAA GGACAGGAAG GCCCACGCGG CGCACCCGGA
    TCAACTGGAA ACCCCGGCGC TCCTGGAACT GTAGGCCCCC CTGGTGCCAG CGGCCCCCCG
    GGAAGTGTGG GCCCCCCTGG CATCAGAGGC CCCCCTGGGA AAGATGGAGA GCGTGGAGAG
    AAGGGGGCGA CAGGTGAAGA AGGGAGTCCA GGGCCAGCTG GTCCCAGGGG TGATCCCGGG
    GCTCCTGGGC TCCCGGGGAC CCCCGGACAG GGAAAAGACG GAGAGCCGGG GCTCCGTGGG
    CCACCTGGAT CACCTGGGCC TCTAGGAATC AAGGGGGACC GAGGTGCACC TGGGATCCCT
    GGCTCTCCTG GCAACCGTGG CGACCCAGGC ATCGGAGTAG CTGGCCCTCC TGGCCCTTCT
    GGACCACCAG GAGACAAAGG GCCCCCGGGA TCCCGAGGCT TACCCGGATT CCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTGTACCA GGAAATCCCG GAGAAAGAGG GCCTCCTGGC
    AAGCCTGGTC CCTCCTCACT GCTGTCTCCA GGGGACATAA ATCTCTTGGT TAAGGATGTG
    TGCAATGACT GCCCTCCAGG ACCCCCGGGC CTCCCTGGCC TCCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GCAAAAAAGG AGATGCTGGG
    CCCAGGGGCC TCCCAGGGCC TCCAGGAATA GCTGGACCAC AGGGAAGTAA AGGGGAGCGT
    GGTGCAGATG GTGAAATTGG ACAGAAAGGT GACCAGGGCC ATCCTGGAGT TCCCGGTTTC
    ATGGGTCCCC CGGGGAACCC TGGGCCACGG GGAGCAGATG GAATTGCCGG AGCTGCTGGC
    CCTCCAGGGA TCCAAGGGCC GCAGGGGAAA GAAGGTCCTC CTGGTCCCCA AGGCCCATTT
    GGATTACCAG GAATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGTCCC
    CCCGGCGAGC CGGGCAAAAT GGGAGAGCCA GGTCTGCCAG GACTGGAGGG AGCCCGAGGC
    CCGCCTGGCT TCAAGGGACA CATGGGTGAC TCCGGCGCAC CTGGTCCCCG GGGAGAGCCT
    GGTGCCATGG GGCCCCCTGG CCGAGAAGGG TCCCCGGGAA AAGACGGCGA CACTGGACCC
    ACAGGGCCCC AGGGCCCCCA AGGACTGAGG GGCCCACCGG GTAAGAACGG ATCGCCGGGA
    TCTCCGGGAG ACCCTGGTCC TGCAGGAAGT CCTGGCCAGA AGGGAAACAA AGGAGACCAT
    GGCAGCCCGG GACTCCCTGG CTTTCTGGGC CCTCGGGGGC CTCAGGGCGA GCCCGGAGAC
    AAGGGAGCCC CGGGCAAGGA GGGCATCCCT GGGAAGCCCG GAGAGCCAGG ACCCAAAGGA
    GACAGGGGAG ATCCCGGGAA CAGAGGTGAC AAAGGACCTC CCGGTGGGAA GGGCCAGCCG
    GGCGACCCTG GAATCCCTGG TCACAAAGGC CTCACGGGCC TGATGGGCCC CCAAGGACCG
    CCCGGGGAGA ATGGAGTGGC CGGGCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGA
    CTCAGGGGGG AGTCCCCCTC CATGGACACG CTGCGACGGC TCATCCAAGA GGAGCTTGAG
    AAACAGCTGG AAACCAGACT CGCCTACCTC CTGGCCCAGA GGCCGCCGGT GCACATGAAG
    TCATCCCAAG GCCGACCCGG GCCCCCGGGG CCCCCGGGAA AAGATGGGCT TCCAGGCCGA
    GCAGGCCCCG TGGGGGAGCC AGGACGGCCC GGGCAGGGGG GTCTGGAAGG ACCCTCTGGA
    CCCATGGGTC CCAAAGGTGA GCGAGGAGCC AAAGGAGACC CAGGTGCGCC TGGAGTGGGC
    CTCCGGGGCG AGATGGGCCC GCCCGGCGTC CCAGGTCAAC CCGGGGAACC TGGCTATGCT
    AAAGACGGGC TCCCCGGGGC TCCCGGCCCT CAAGGGGAGA CCGGACCAGC GGGACACCCT
    GGCCCCCCGG GACCGCCCGG GCCCCCTGGC CAGTGCGACC CCTCCCAGTG TGCCTACTTT
    GCCAGCCTCG CTGCACGGCC CAGTAACGTG AAGGGCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282449.1
    CDS623..5344
    Translation

    Target ORF information:

    RefSeq Version XM_022426741.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426741.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG TGAAGCTGGT CCTGCAGGCC CCCCTGGATT ACCTGGCACT
    ACATCCCTGT TCACACCGCA CCCAAGAATG CCAGGAGAGC AAGGGCCCAA AGGAGAGAAG
    GGTGATCCTG GCGTGCCTGG GGAACCGGGA TTGCAGGGCC ATCCCGGAGA ACTGGGGCCT
    CGGGGACCCA CTGGAGCGCC GGGTGCCAAA GGACAGGAAG GCCCACGCGG CGCACCCGGA
    TCAACTGGAA ACCCCGGCGC TCCTGGAACT GTAGGCCCCC CTGGTGCCAG CGGCCCCCCG
    GGAAGTGTGG GCCCCCCTGG CATCAGAGGC CCCCCTGGGA AAGATGGAGA GCGTGGAGAG
    AAGGGGGCGA CAGGTGAAGA AGGGAGTCCA GGGCCAGCTG GTCCCAGGGG TGATCCCGGG
    GCTCCTGGGC TCCCGGGGAC CCCCGGACAG GGAAAAGACG GAGAGCCGGG GCTCCGTGGG
    CCACCTGGAT CACCTGGGCC TCTAGGAATC AAGGGGGACC GAGGTGCACC TGGGATCCCT
    GGCTCTCCTG GCAACCGTGG CGACCCAGGC ATCGGAGTAG CTGGCCCTCC TGGCCCTTCT
    GGACCACCAG GAGACAAAGG GCCCCCGGGA TCCCGAGGCT TACCCGGATT CCCCGGCCCC
    CAGGGACCAG CTGGCCAGGA TGGTGTACCA GGAAATCCCG GAGAAAGAGG GCCTCCTGGC
    AAGCCTGGTC CCTCCTCACT GCTGTCTCCA GGGGACATAA ATCTCTTGGT TAAGGATGTG
    TGCAATGACT GCCCTCCAGG ACCCCCGGGC CTCCCTGGCC TCCCAGGTTT TAAAGGGGAC
    AAAGGTCTCC CAGGAAAGCC AGGGAGAGAA GGCACAGAAG GCAAAAAAGG AGATGCTGGG
    CCCAGGGGCC TCCCAGGGCC TCCAGGAATA GCTGGACCAC AGGGAAGTAA AGGGGAGCGT
    GGTGCAGATG GTGAAATTGG ACAGAAAGGT GACCAGGGCC ATCCTGGAGT TCCCGGTTTC
    ATGGGTCCCC CGGGGAACCC TGGGCCACGG GGAGCAGATG GAATTGCCGG AGCTGCTGGC
    CCTCCAGGGA TCCAAGGGCC GCAGGGGAAA GAAGGTCCTC CTGGTCCCCA AGGCCCATTT
    GGATTACCAG GAATCCCAGG AGAAGAAGGC AAAGAGGGCA GAGATGGAAA ACCAGGTCCC
    CCCGGCGAGC CGGGCAAAAT GGGAGAGCCA GGTCTGCCAG GACTGGAGGG AGCCCGAGGC
    CCGCCTGGCT TCAAGGGACA CATGGGTGAC TCCGGCGCAC CTGGTCCCCG GGGAGAGCCT
    GGTGCCATGG GGCCCCCTGG CCGAGAAGGG TCCCCGGGAA AAGACGGCGA CACTGGACCC
    ACAGGGCCCC AGGGCCCCCA AGGACTGAGG GGCCCACCGG GTAAGAACGG ATCGCCGGGA
    TCTCCGGGAG ACCCTGGTCC TGCAGGAAGT CCTGGCCAGA AGGGAAACAA AGGAGACCAT
    GGCAGCCCGG GACTCCCTGG CTTTCTGGGC CCTCGGGGGC CTCAGGGCGA GCCCGGAGAC
    AAGGGAGCCC CGGGCAAGGA GGGCATCCCT GGGAAGCCCG GAGAGCCAGG ACCCAAAGGA
    GACAGGGGAG ATCCCGGGAA CAGAGGTGAC AAAGGACCTC CCGGTGGGAA GGGCCAGCCG
    GGCGACCCTG GAATCCCTGG TCACAAAGGC CTCACGGGCC TGATGGGCCC CCAAGGACCG
    CCCGGGGAGA ATGGAGTGGC CGGGCCCCCA GGGCCTCCGG GCCAGCCAGG ATTTCCAGGA
    CTCAGGGGGG AGTCCCCCTC CATGGACACG CTGCGACGGC TCATCCAAGA GGAGCTTGAG
    AAACAGCTGG AAACCAGACT CGCCTACCTC CTGGCCCAGA GGCCGCCGGT GCACATGAAG
    TCATCCCAAG GCCGACCCGG GCCCCCGGGG CCCCCGGGAA AAGATGGGCT TCCAGGCCGA
    GCAGGCCCCG TGGGGGAGCC AGGACGGCCC GGGCAGGGGG GTCTGGAAGG ACCCTCTGGA
    CCCATGGGTC CCAAAGGTGA GCGAGGAGCC AAAGGAGACC CAGGTGCGCC TGGAGTGGGC
    CTCCGGGGCG AGATGGGCCC GCCCGGCGTC CCAGGTCAAC CCGGGGAACC TGGCTATGCT
    AAAGACGGGC TCCCCGGGGC TCCCGGCCCT CAAGGGGAGA CCGGACCAGC GGGACACCCT
    GGCCCCCCGG GACCGCCCGG GCCCCCTGGC CAGTGCGACC CCTCCCAGTG TGCCTACTTT
    GCCAGCCTCG CTGCACGGCC CAGTAACGTG AAGGGCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292866
    Clone ID Related Accession (Same CDS sequence) XM_022426742.1
    Accession Version XM_022426742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4659bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-1(XXII) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282450.1
    CDS622..5280
    Translation

    Target ORF information:

    RefSeq Version XM_022426742.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCATGTTC CCCAGCGTGG GAGAGCCGTG GCCAGCCTGC GAGGGAACGC CGTGGCCTGC 
    CTCCTCTGGA TGTTGCTGCT GTGGCGTGGG GACGGCTGTC AGGCCCAGAG GGCAGGTTGC
    AAGACGGTCC ACTATGACCT GGTCTTCCTC TTGGACACCT CCTCCAGCGT GGGCAAGGAG
    GACTTCGAGA AGGTCCGGCA GTGGGTGGCC AACCTGGTGG ACACCTTCGA GGTGGGCCCC
    GACCGCACCC GCGTGGGGGT CGTGCGCTAC AGCGACCGGC CCACCACGGC CTTCGAACTG
    GGCCTGTTCG GCTCCCGGGA GGCTGTCAAG GCGGCGGCGC GGCACCTGGC CTACCACGGC
    GGCAACACCA ACACGGGCGA CGCCCTGCGC TTCATCACGC GCCACAGCTT CTCCCGGCAG
    GCCGGTGGCC GCCCCGGGGA CCGCGCCTTC AAGCAGGTGG CCATCCTCCT GACCGACGGC
    CGCAGCCAGG ACCTGGTGCT GGACGCCGCG GCCACCGCGC ACCGCGCGGG CATCCGCATC
    TTCGCCGTGG GCGTCGGGGC GGCGCTCAGG GAGGAGCTGG AGGAGATCGC CTCGGAGCCC
    AAGTCTGCCC ACGTCTTCCA CGTGTCCGAC TTCGACGCCA TCGACAAGAT CCGTGGCAAG
    CTGAGGCGCC GCCTGTGTGA AAACGTGCTG TGCCCCAGCG TCCGTGTGGA AGGAGACCGC
    TTTAAGCACA TCAACGGTGG AAGCAAGGAA ATCACAGGTT TTGACCTGAT GGATTCATTC
    AGGGTGAAGG AGATCCTGGG GAGGAGAGAC AACGGGGTGC AGAGCTCCTA TGTCCGGATG
    GGATCCTTCC CTGTGGTACA GAGGACAGAG GATGTGTTCC CTCAAGGCTT GCCTGATGAA
    TACGCCTTTG TCACCACCTT CCGGTTCAGG AAAACATCTC GGAAAGAAGA TTGGTACATC
    TGGCAGGTCA TCGACCAGTA TGGGATCCCG CAGGTCTCCA TCCGCCTGGA CGGCGAGAAC
    AAGGCAGTCG AGTATGACGC CGTGGGTGCC ATGAAGGACG CCGTCAGGGT GGTCTTCCGA
    GGCCCCCGGG TGGGTGACCT CTTTGACCGG GACTGGCACA AGGTGGCCCT GAGCATCCAG
    GCCCAGAACG TCTCCCTGTA CGTCGACTGT GCGCTGGTGC AGACGCTGCC CATCGAGGAG
    AGGGAGAACA TCGACATCCA GGGGAAGACC GTGATCGGCA AGCGCCTCTA CGACAGCGTG
    CCCATCGATT TCGACCTGCA GCGGATTGTG ATTTACTGTG ACTCCCGACA TGCAGAATTG
    GAGACTTGCT GCGATATCCC CCTGGGCCCG TGCCAAGTGA CCGTGCTGAC GGAGCCTTCA
    CCGCCGCCCC CGCGGCCTCC CACGCCCGGC AGTGAACAGA TCGGGTTTTT GAAGACCATC
    AACTGCTCCT GCCCGGCTGG AGAAAAGGGT GAAAAGGGCT CTGATGGCCC CGTGGGACTC
    CCTGGCCCAA AGGGAGACAT GGGAGCCACT GGGTTGCTCG GAGCTCCTGG ACCCAAGGGA
    GAAAAAGGCG ACATGGGCAG AGGACCTTTT TTCCCAGGAG AGAAGGGTGA AAAGGGTTCC
    CTGGGCCCGC CTGGTCCCCC TGGGAGAGAC GGCAGCAAAG GCATGCGGGG GGAGCCAGGA
    GAGCCAGGAG AGCCAGGGCT GCCCGGCGAG GTGGGCATGC GGGGATTCCA AGGACCACCT
    GGACTCCCCG GGCCTCCTGG ACACGTTGGA GCTCCTGGTC TCCAAGGAGA ACGGGGTGAA
    AAGGGAGCCC GAGGAGAAAA GGGGGAGCGA GGCCTGGATG GATTCCCCGG GAAGCGTGGA
    GAGACAGGAG AGCAGGGAAG ACCTGGCCCT CCTGGTGTGG CAGGACTCCA GGGACAGAAG
    GGTGACGTGG GGCCTGCAGG TCCTCCTGGT GTCCCGGGCT CTGTGGTGCA GAGAGAGGGT
    CTGAAGGGCG AGCAGGGAGC TCCAGGACCA CGGGGTCACC AGGGCCCACC TGGGCCACCA
    GGAGCTCCGG GCCTGATGGG TCCTGAAGGC AAGGATGGAC CCCCTGGTTT GCAAGGTCTC
    CGGGGGAAGA AAGGTGAAGT GGGCCCGCCG GGAACCCCTG GACTGCTGGG GCCACAGGGC
    CCACCAGGAC CGCCTGGCAT CCCAGGCCCC CCCGGACCCG GAGGACCCCC GGGTTTACCT
    GGAGAGATCG GCTTCCCGGG AAAACCCGGG CACCCCGGGC CGACGGGCAC ACCTGGAAAG
    GATGGGCAGG ATGGAGCACC AGGCCTGCCA GGACCCAAGG GCCAGCCAGG AGACAGAGGA
    GAAGACGGTC TGCCTGGAAA ACCCGGCCTT AGGGGTGAAG TTGGGGAGCA AGGCCTGGCA
    GGCCGACCTG GAGAGAAGGG AGCAGCGGGG CTCCCAGGGG CTCCCGGATT CCCAGGCGTG
    AGGGGAGAGA AAGGAGAGCA GGGAGAGAAA GGTGGGCTGG GCCTCCCGGG AGTGAAAGGT
    GACCCAGGTG AAAAGGGTGA AGCTGGTCCT GCAGGCCCCC CTGGATTACC TGGCACTACA
    TCCCTGTTCA CACCGCACCC AAGAATGCCA GGAGAGCAAG GGCCCAAAGG AGAGAAGGGT
    GATCCTGGCG TGCCTGGGGA ACCGGGATTG CAGGGCCATC CCGGAGAACT GGGGCCTCGG
    GGACCCACTG GAGCGCCGGG TGCCAAAGGA CAGGAAGGCC CACGCGGCGC ACCCGGATCA
    ACTGGAAACC CCGGCGCTCC TGGAACTGTA GGCCCCCCTG GTGCCAGCGG CCCCCCGGGA
    AGTGTGGGCC CCCCTGGCAT CAGAGGCCCC CCTGGGAAAG ATGGAGAGCG TGGAGAGAAG
    GGGGCGACAG GTGAAGAAGG GAGTCCAGGG CCAGCTGGTC CCAGGGGTGA TCCCGGGGCT
    CCTGGGCTCC CGGGGACCCC CGGACAGGGA AAAGACGGAG AGCCGGGGCT CCGTGGGCCA
    CCTGGATCAC CTGGGCCTCT AGGAATCAAG GGGGACCGAG GTGCACCTGG GATCCCTGGC
    TCTCCTGGCA ACCGTGGCGA CCCAGGCATC GGAGTAGCTG GCCCTCCTGG CCCTTCTGGA
    CCACCAGGAG ACAAAGGGCC CCCGGGATCC CGAGGCTTAC CCGGATTCCC CGGCCCCCAG
    GGACCAGCTG GCCAGGATGG TGTACCAGGA AATCCCGGAG AAAGAGGGCC TCCTGGCAAG
    CCTGGTCCCT CCTCACTGCT GTCTCCAGGG GACATAAATC TCTTGGTTAA GGATGTGTGC
    AATGACTGCC CTCCAGGACC CCCGGGCCTC CCTGGCCTCC CAGGTTTTAA AGGGGACAAA
    GGTCTCCCAG GAAAGCCAGG GAGAGAAGGC ACAGAAGGCA AAAAAGGAGA TGCTGGGCCC
    AGGGGCCTCC CAGGGCCTCC AGGAATAGCT GGACCACAGG GAAGTAAAGG GGAGCGTGGT
    GCAGATGGTG AAATTGGACA GAAAGGTGAC CAGGGCCATC CTGGAGTTCC CGGTTTCATG
    GGTCCCCCGG GGAACCCTGG GCCACGGGGA GCAGATGGAA TTGCCGGAGC TGCTGGCCCT
    CCAGGGATCC AAGGGCCGCA GGGGAAAGAA GGTCCTCCTG GTCCCCAAGG CCCATTTGGA
    TTACCAGGAA TCCCAGGAGA AGAAGGCAAA GAGGGCAGAG ATGGAAAACC AGGTCCCCCC
    GGCGAGCCGG GCAAAATGGG AGAGCCAGGT CTGCCAGGAC TGGAGGGAGC CCGAGGCCCG
    CCTGGCTTCA AGGGACACAT GGGTGACTCC GGCGCACCTG GTCCCCGGGG AGAGCCTGGT
    GCCATGGGGC CCCCTGGCCG AGAAGGGTCC CCGGGAAAAG ACGGCGACAC TGGACCCACA
    GGGCCCCAGG GCCCCCAAGG ACTGAGGGGC CCACCGGGTA AGAACGGATC GCCGGGATCT
    CCGGGAGACC CTGGTCCTGC AGGAAGTCCT GGCCAGAAGG GAAACAAAGG AGACCATGGC
    AGCCCGGGAC TCCCTGGCTT TCTGGGCCCT CGGGGGCCTC AGGGCGAGCC CGGAGACAAG
    GGAGCCCCGG GCAAGGAGGG CATCCCTGGG AAGCCCGGAG AGCCAGGACC CAAAGGAGAC
    AGGGGAGATC CCGGGAACAG AGGTGACAAA GGACCTCCCG GTGGGAAGGG CCAGCCGGGC
    GACCCTGGAA TCCCTGGTCA CAAAGGCCTC ACGGGCCTGA TGGGCCCCCA AGGACCGCCC
    GGGGAGAATG GAGTGGCCGG GCCCCCAGGG CCTCCGGGCC AGCCAGGATT TCCAGGACTC
    AGGGGGGAGT CCCCCTCCAT GGACACGCTG CGACGGCTCA TCCAAGAGGA GCTTGAGAAA
    CAGCTGGAAA CCAGACTCGC CTACCTCCTG GCCCAGAGGC CGCCGGTGCA CATGAAGTCA
    TCCCAAGGCC GACCCGGGCC CCCGGGGCCC CCGGGAAAAG ATGGGCTTCC AGGCCGAGCA
    GGCCCCGTGG GGGAGCCAGG ACGGCCCGGG CAGGGGGGTC TGGAAGGACC CTCTGGACCC
    ATGGGTCCCA AAGGTGAGCG AGGAGCCAAA GGAGACCCAG GTGCGCCTGG AGTGGGCCTC
    CGGGGCGAGA TGGGCCCGCC CGGCGTCCCA GGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292867
    Clone ID Related Accession (Same CDS sequence) XM_022426743.1
    Accession Version XM_022426743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-1(XXII) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTGGG GCCCAGGGGA TGTGTTCCCT CAAGGCTTGC CTGATGAATA CGCCTTTGTC 
    ACCACCTTCC GGTTCAGGAA AACATCTCGG AAAGAAGATT GGTACATCTG GCAGGTCATC
    GACCAGTATG GGATCCCGCA GGTCTCCATC CGCCTGGACG GCGAGAACAA GGCAGTCGAG
    TATGACGCCG TGGGTGCCAT GAAGGACGCC GTCAGGGTGG TCTTCCGAGG CCCCCGGGTG
    GGTGACCTCT TTGACCGGGA CTGGCACAAG GTGGCCCTGA GCATCCAGGC CCAGAACGTC
    TCCCTGTACG TCGACTGTGC GCTGGTGCAG ACGCTGCCCA TCGAGGAGAG GGAGAACATC
    GACATCCAGG GGAAGACCGT GATCGGCAAG CGCCTCTACG ACAGCGTGCC CATCGATTTC
    GACCTGCAGC GGATTGTGAT TTACTGTGAC TCCCGACATG CAGAATTGGA GACTTGCTGC
    GATATCCCCC TGGGCCCGTG CCAAGTGACC GTGCTGACGG AGCCTTCACC GCCGCCCCCG
    CGGCCTCCCA CGCCCGGCAG TGAACAGATC GGGTTTTTGA AGACCATCAA CTGCTCCTGC
    CCGGCTGGAG AAAAGGGTGA AAAGGGCTCT GATGGCCCCG TGGGACTCCC TGGCCCAAAG
    GGAGACATGG GAGCCACTGG GTTGCTCGGA GCTCCTGGAC CCAAGGGAGA AAAAGGCGAC
    ATGGGCAGAG GACCTTTTTT CCCAGGAGAG AAGGGTGAAA AGGGTTCCCT GGGCCCGCCT
    GGTCCCCCTG GGAGAGACGG CAGCAAAGGC ATGCGGGGGG AGCCAGGAGA GCCAGGAGAG
    CCAGGGCTGC CCGGCGAGGT GGGCATGCGG GGATTCCAAG GACCACCTGG ACTCCCCGGG
    CCTCCTGGAC ACGTTGGAGC TCCTGGTCTC CAAGGAGAAC GGGGTGAAAA GGGAGCCCGA
    GGAGAAAAGG GGGAGCGAGG CCTGGATGGA TTCCCCGGGA AGCGTGGAGA GACAGGAGAG
    CAGGGAAGAC CTGGCCCTCC TGGTGTGGCA GGACTCCAGG GACAGAAGGG TGACGTGGGG
    CCTGCAGGTC CTCCTGGTGT CCCGGGCTCT GTGGTGCAGA GAGAGGGTCT GAAGGGCGAG
    CAGGGAGCTC CAGGACCACG GGGTCACCAG GGCCCACCTG GGCCACCAGG AGCTCCGGGC
    CTGATGGGTC CTGAAGGCAA GGATGGACCC CCTGGTTTGC AAGGTCTCCG GGGGAAGAAA
    GGTGAAGTGG GCCCGCCGGG AACCCCTGGA CTGCTGGGGC CACAGGGCCC ACCAGGACCG
    CCTGGCATCC CAGGCCCCCC CGGACCCGGA GGACCCCCGG GTTTACCTGG AGAGATCGGC
    TTCCCGGGAA AACCCGGGCA CCCCGGGCCG ACGGGCACAC CTGGAAAGGA TGGGCAGGAT
    GGAGCACCAG GCCTGCCAGG ACCCAAGGGC CAGCCAGGAG ACAGAGGAGA AGACGGTCTG
    CCTGGAAAAC CCGGCCTTAG GGGTGAAGTT GGGGAGCAAG GCCTGGCAGG CCGACCTGGA
    GAGAAGGGAG CAGCGGGGCT CCCAGGGGCT CCCGGATTCC CAGGCGTGAG GGGAGAGAAA
    GGAGAGCAGG GAGAGAAAGG TGGGCTGGGC CTCCCGGGAG TGAAAGGTGA CCCAGGTGAA
    AAGGGTGAAG CTGGTCCTGC AGGCCCCCCT GGATTACCTG GCACTACATC CCTGTTCACA
    CCGCACCCAA GAATGCCAGG AGAGCAAGGG CCCAAAGGAG AGAAGGGTGA TCCTGGCGTG
    CCTGGGGAAC CGGGATTGCA GGGCCATCCC GGAGAACTGG GGCCTCGGGG ACCCACTGGA
    GCGCCGGGTG CCAAAGGACA GGAAGGCCCA CGCGGCGCAC CCGGATCAAC TGGAAACCCC
    GGCGCTCCTG GAACTGTAGG CCCCCCTGGT GCCAGCGGCC CCCCGGGAAG TGTGGGCCCC
    CCTGGCATCA GAGGCCCCCC TGGGAAAGAT GGAGAGCGTG GAGAGAAGGG GGCGACAGGT
    GAAGAAGGGA GTCCAGGGCC AGCTGGTCCC AGGGGTGATC CCGGGGCTCC TGGGCTCCCG
    GGGACCCCCG GACAGGGAAA AGACGGAGAG CCGGGGCTCC GTGGGCCACC TGGATCACCT
    GGGCCTCTAG GAATCAAGGG GGACCGAGGT GCACCTGGGA TCCCTGGCTC TCCTGGCAAC
    CGTGGCGACC CAGGCATCGG AGTAGCTGGC CCTCCTGGCC CTTCTGGACC ACCAGGAGAC
    AAAGGGCCCC CGGGATCCCG AGGCTTACCC GGATTCCCCG GCCCCCAGGG ACCAGCTGGC
    CAGGATGGTG TACCAGGAAA TCCCGGAGAA AGAGGGCCTC CTGGCAAGCC TGGTCCCTCC
    TCACTGCTGT CTCCAGGGGA CATAAATCTC TTGGTTAAGG ATGTGTGCAA TGACTGCCCT
    CCAGGACCCC CGGGCCTCCC TGGCCTCCCA GGTTTTAAAG GGGACAAAGG TCTCCCAGGA
    AAGCCAGGGA GAGAAGGCAC AGAAGGCAAA AAAGGAGATG CTGGGCCCAG GGGCCTCCCA
    GGGCCTCCAG GAATAGCTGG ACCACAGGGA AGTAAAGGGG AGCGTGGTGC AGATGGTGAA
    ATTGGACAGA AAGGTGACCA GGGCCATCCT GGAGTTCCCG GTTTCATGGG TCCCCCGGGG
    AACCCTGGGC CACGGGGAGC AGATGGAATT GCCGGAGCTG CTGGCCCTCC AGGGATCCAA
    GGGCCGCAGG GGAAAGAAGG TCCTCCTGGT CCCCAAGGCC CATTTGGATT ACCAGGAATC
    CCAGGAGAAG AAGGCAAAGA GGGCAGAGAT GGAAAACCAG GTCCCCCCGG CGAGCCGGGC
    AAAATGGGAG AGCCAGGTCT GCCAGGACTG GAGGGAGCCC GAGGCCCGCC TGGCTTCAAG
    GGACACATGG GTGACTCCGG CGCACCTGGT CCCCGGGGAG AGCCTGGTGC CATGGGGCCC
    CCTGGCCGAG AAGGGTCCCC GGGAAAAGAC GGCGACACTG GACCCACAGG GCCCCAGGGC
    CCCCAAGGAC TGAGGGGCCC ACCGGGTAAG AACGGATCGC CGGGATCTCC GGGAGACCCT
    GGTCCTGCAG GAAGTCCTGG CCAGAAGGGA AACAAAGGAG ACCATGGCAG CCCGGGACTC
    CCTGGCTTTC TGGGCCCTCG GGGGCCTCAG GGCGAGCCCG GAGACAAGGG AGCCCCGGGC
    AAGGAGGGCA TCCCTGGGAA GCCCGGAGAG CCAGGACCCA AAGGAGACAG GGGAGATCCC
    GGGAACAGAG GTGACAAAGG ACCTCCCGGT GGGAAGGGCC AGCCGGGCGA CCCTGGAATC
    CCTGGTCACA AAGGCCTCAC GGGCCTGATG GGCCCCCAAG GACCGCCCGG GGAGAATGGA
    GTGGCCGGGC CCCCAGGGCC TCCGGGCCAG CCAGGATTTC CAGGACTCAG GGGGGAGTCC
    CCCTCCATGG ACACGCTGCG ACGGCTCATC CAAGAGGAGC TTGAGAAACA GCTGGAAACC
    AGACTCGCCT ACCTCCTGGC CCAGAGGCCG CCGGTGCACA TGAAGTCATC CCAAGGCCGA
    CCCGGGCCCC CGGGGCCCCC GGGAAAAGAT GGGCTTCCAG GCCGAGCAGG CCCCGTGGGG
    GAGCCAGGAC GGCCCGGGCA GGGGGGTCTG GAAGGACCCT CTGGACCCAT GGGTCCCAAA
    GGTGAGCGAG GAGCCAAAGG AGACCCAGGT GCGCCTGGAG TGGGCCTCCG GGGCGAGATG
    GGCCCGCCCG GCGTCCCAGG TCAACCCGGG GAACCTGGCT ATGCTAAAGA CGGGCTCCCC
    GGGGCTCCCG GCCCTCAAGG GGAGACCGGA CCAGCGGGAC ACCCTGGCCC CCCGGGACCG
    CCCGGGCCCC CTGGCCAGTG CGACCCCTCC CAGTGTGCCT ACTTTGCCAG CCTCGCTGCA
    CGGCCCAGTA ACGTGAAGGG CCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282451.1
    CDS96..4082
    Translation

    Target ORF information:

    RefSeq Version XM_022426743.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type XXII alpha 1 chain (COL22A1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTGGG GCCCAGGGGA TGTGTTCCCT CAAGGCTTGC CTGATGAATA CGCCTTTGTC 
    ACCACCTTCC GGTTCAGGAA AACATCTCGG AAAGAAGATT GGTACATCTG GCAGGTCATC
    GACCAGTATG GGATCCCGCA GGTCTCCATC CGCCTGGACG GCGAGAACAA GGCAGTCGAG
    TATGACGCCG TGGGTGCCAT GAAGGACGCC GTCAGGGTGG TCTTCCGAGG CCCCCGGGTG
    GGTGACCTCT TTGACCGGGA CTGGCACAAG GTGGCCCTGA GCATCCAGGC CCAGAACGTC
    TCCCTGTACG TCGACTGTGC GCTGGTGCAG ACGCTGCCCA TCGAGGAGAG GGAGAACATC
    GACATCCAGG GGAAGACCGT GATCGGCAAG CGCCTCTACG ACAGCGTGCC CATCGATTTC
    GACCTGCAGC GGATTGTGAT TTACTGTGAC TCCCGACATG CAGAATTGGA GACTTGCTGC
    GATATCCCCC TGGGCCCGTG CCAAGTGACC GTGCTGACGG AGCCTTCACC GCCGCCCCCG
    CGGCCTCCCA CGCCCGGCAG TGAACAGATC GGGTTTTTGA AGACCATCAA CTGCTCCTGC
    CCGGCTGGAG AAAAGGGTGA AAAGGGCTCT GATGGCCCCG TGGGACTCCC TGGCCCAAAG
    GGAGACATGG GAGCCACTGG GTTGCTCGGA GCTCCTGGAC CCAAGGGAGA AAAAGGCGAC
    ATGGGCAGAG GACCTTTTTT CCCAGGAGAG AAGGGTGAAA AGGGTTCCCT GGGCCCGCCT
    GGTCCCCCTG GGAGAGACGG CAGCAAAGGC ATGCGGGGGG AGCCAGGAGA GCCAGGAGAG
    CCAGGGCTGC CCGGCGAGGT GGGCATGCGG GGATTCCAAG GACCACCTGG ACTCCCCGGG
    CCTCCTGGAC ACGTTGGAGC TCCTGGTCTC CAAGGAGAAC GGGGTGAAAA GGGAGCCCGA
    GGAGAAAAGG GGGAGCGAGG CCTGGATGGA TTCCCCGGGA AGCGTGGAGA GACAGGAGAG
    CAGGGAAGAC CTGGCCCTCC TGGTGTGGCA GGACTCCAGG GACAGAAGGG TGACGTGGGG
    CCTGCAGGTC CTCCTGGTGT CCCGGGCTCT GTGGTGCAGA GAGAGGGTCT GAAGGGCGAG
    CAGGGAGCTC CAGGACCACG GGGTCACCAG GGCCCACCTG GGCCACCAGG AGCTCCGGGC
    CTGATGGGTC CTGAAGGCAA GGATGGACCC CCTGGTTTGC AAGGTCTCCG GGGGAAGAAA
    GGTGAAGTGG GCCCGCCGGG AACCCCTGGA CTGCTGGGGC CACAGGGCCC ACCAGGACCG
    CCTGGCATCC CAGGCCCCCC CGGACCCGGA GGACCCCCGG GTTTACCTGG AGAGATCGGC
    TTCCCGGGAA AACCCGGGCA CCCCGGGCCG ACGGGCACAC CTGGAAAGGA TGGGCAGGAT
    GGAGCACCAG GCCTGCCAGG ACCCAAGGGC CAGCCAGGAG ACAGAGGAGA AGACGGTCTG
    CCTGGAAAAC CCGGCCTTAG GGGTGAAGTT GGGGAGCAAG GCCTGGCAGG CCGACCTGGA
    GAGAAGGGAG CAGCGGGGCT CCCAGGGGCT CCCGGATTCC CAGGCGTGAG GGGAGAGAAA
    GGAGAGCAGG GAGAGAAAGG TGGGCTGGGC CTCCCGGGAG TGAAAGGTGA CCCAGGTGAA
    AAGGGTGAAG CTGGTCCTGC AGGCCCCCCT GGATTACCTG GCACTACATC CCTGTTCACA
    CCGCACCCAA GAATGCCAGG AGAGCAAGGG CCCAAAGGAG AGAAGGGTGA TCCTGGCGTG
    CCTGGGGAAC CGGGATTGCA GGGCCATCCC GGAGAACTGG GGCCTCGGGG ACCCACTGGA
    GCGCCGGGTG CCAAAGGACA GGAAGGCCCA CGCGGCGCAC CCGGATCAAC TGGAAACCCC
    GGCGCTCCTG GAACTGTAGG CCCCCCTGGT GCCAGCGGCC CCCCGGGAAG TGTGGGCCCC
    CCTGGCATCA GAGGCCCCCC TGGGAAAGAT GGAGAGCGTG GAGAGAAGGG GGCGACAGGT
    GAAGAAGGGA GTCCAGGGCC AGCTGGTCCC AGGGGTGATC CCGGGGCTCC TGGGCTCCCG
    GGGACCCCCG GACAGGGAAA AGACGGAGAG CCGGGGCTCC GTGGGCCACC TGGATCACCT
    GGGCCTCTAG GAATCAAGGG GGACCGAGGT GCACCTGGGA TCCCTGGCTC TCCTGGCAAC
    CGTGGCGACC CAGGCATCGG AGTAGCTGGC CCTCCTGGCC CTTCTGGACC ACCAGGAGAC
    AAAGGGCCCC CGGGATCCCG AGGCTTACCC GGATTCCCCG GCCCCCAGGG ACCAGCTGGC
    CAGGATGGTG TACCAGGAAA TCCCGGAGAA AGAGGGCCTC CTGGCAAGCC TGGTCCCTCC
    TCACTGCTGT CTCCAGGGGA CATAAATCTC TTGGTTAAGG ATGTGTGCAA TGACTGCCCT
    CCAGGACCCC CGGGCCTCCC TGGCCTCCCA GGTTTTAAAG GGGACAAAGG TCTCCCAGGA
    AAGCCAGGGA GAGAAGGCAC AGAAGGCAAA AAAGGAGATG CTGGGCCCAG GGGCCTCCCA
    GGGCCTCCAG GAATAGCTGG ACCACAGGGA AGTAAAGGGG AGCGTGGTGC AGATGGTGAA
    ATTGGACAGA AAGGTGACCA GGGCCATCCT GGAGTTCCCG GTTTCATGGG TCCCCCGGGG
    AACCCTGGGC CACGGGGAGC AGATGGAATT GCCGGAGCTG CTGGCCCTCC AGGGATCCAA
    GGGCCGCAGG GGAAAGAAGG TCCTCCTGGT CCCCAAGGCC CATTTGGATT ACCAGGAATC
    CCAGGAGAAG AAGGCAAAGA GGGCAGAGAT GGAAAACCAG GTCCCCCCGG CGAGCCGGGC
    AAAATGGGAG AGCCAGGTCT GCCAGGACTG GAGGGAGCCC GAGGCCCGCC TGGCTTCAAG
    GGACACATGG GTGACTCCGG CGCACCTGGT CCCCGGGGAG AGCCTGGTGC CATGGGGCCC
    CCTGGCCGAG AAGGGTCCCC GGGAAAAGAC GGCGACACTG GACCCACAGG GCCCCAGGGC
    CCCCAAGGAC TGAGGGGCCC ACCGGGTAAG AACGGATCGC CGGGATCTCC GGGAGACCCT
    GGTCCTGCAG GAAGTCCTGG CCAGAAGGGA AACAAAGGAG ACCATGGCAG CCCGGGACTC
    CCTGGCTTTC TGGGCCCTCG GGGGCCTCAG GGCGAGCCCG GAGACAAGGG AGCCCCGGGC
    AAGGAGGGCA TCCCTGGGAA GCCCGGAGAG CCAGGACCCA AAGGAGACAG GGGAGATCCC
    GGGAACAGAG GTGACAAAGG ACCTCCCGGT GGGAAGGGCC AGCCGGGCGA CCCTGGAATC
    CCTGGTCACA AAGGCCTCAC GGGCCTGATG GGCCCCCAAG GACCGCCCGG GGAGAATGGA
    GTGGCCGGGC CCCCAGGGCC TCCGGGCCAG CCAGGATTTC CAGGACTCAG GGGGGAGTCC
    CCCTCCATGG ACACGCTGCG ACGGCTCATC CAAGAGGAGC TTGAGAAACA GCTGGAAACC
    AGACTCGCCT ACCTCCTGGC CCAGAGGCCG CCGGTGCACA TGAAGTCATC CCAAGGCCGA
    CCCGGGCCCC CGGGGCCCCC GGGAAAAGAT GGGCTTCCAG GCCGAGCAGG CCCCGTGGGG
    GAGCCAGGAC GGCCCGGGCA GGGGGGTCTG GAAGGACCCT CTGGACCCAT GGGTCCCAAA
    GGTGAGCGAG GAGCCAAAGG AGACCCAGGT GCGCCTGGAG TGGGCCTCCG GGGCGAGATG
    GGCCCGCCCG GCGTCCCAGG TCAACCCGGG GAACCTGGCT ATGCTAAAGA CGGGCTCCCC
    GGGGCTCCCG GCCCTCAAGG GGAGACCGGA CCAGCGGGAC ACCCTGGCCC CCCGGGACCG
    CCCGGGCCCC CTGGCCAGTG CGACCCCTCC CAGTGTGCCT ACTTTGCCAG CCTCGCTGCA
    CGGCCCAGTA ACGTGAAGGG CCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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